LOCUS       DSEB01000001            6066 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000867, whole genome shotgun sequence.
ACCESSION   DSEB01000001 DSEB01000000
VERSION     DSEB01000001.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000867"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..388
                     /locus_tag="ENO07_00005"
     CDS             <1..388
                     /locus_tag="ENO07_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDS00376.1"
                     /translation="TFTLLIGSIAGISLLVGGIGILAIMLIAIRERTNEIGLRMAVGA
                     SRKDIMTQFIMEASILGVSGGLVGIVLGILGSILVSAGTSWAISISSESVLLAFGCSL
                     LAGLFFGVYPAHRASMLDPIDALRSE"
     gene            complement(458..1825)
                     /locus_tag="ENO07_00010"
     CDS             complement(458..1825)
                     /locus_tag="ENO07_00010"
                     /inference="COORDINATES: protein motif:HMM:PF10011.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HDS00377.1"
                     /translation="MKTKLLNVWESIRTSFYFIPGLMVAIATGLAIIMVYFDRQFEPD
                     SYGFLSFFYSGGVDGARSILATIAGSMITVAGVTFSITIVALTLTTSQFGPRLLRNFV
                     RHPGNQVVLGAFVGTFIYCLLVLGSIHDQEGYLFVPILSVNLSILLVIANVGILVYFI
                     HHVSASIQADNVVQAVYNELLERLLPLFPDEIADDTGSTGELDQDRQARINSYRKSRS
                     ISAPKDGYLQAVDINGLLRIAIATDSLLSLEYRPGEYITADSTLVTVSFDGQFSDESA
                     EDLANSFFVGPQRSPLQDAEYAIHQLVEIAVRALSPGINDPFTAISCIDRLGSAVSFL
                     AGRSFPSSHHCEDEGKLRLVTKPWTFTGVVNAAFDQIRQNSQSNVAVTTRLLETLTMI
                     AGVCRNAEQREALLRQGNMILRASEEFISEKDDKQDVQQRYQALGDALSRSADSGDPD
                     NCQAK"
     gene            complement(1857..2075)
                     /locus_tag="ENO07_00015"
     CDS             complement(1857..2075)
                     /locus_tag="ENO07_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00378.1"
                     /translation="MPDRTNELKARVEAKKKELEQNLTQLKAEAHGKKNDEIEKLENQ
                     LNELNELIKDGWDNISEAAVEKINSWLQ"
     gene            2529..2927
                     /locus_tag="ENO07_00020"
     CDS             2529..2927
                     /locus_tag="ENO07_00020"
                     /inference="COORDINATES: protein motif:HMM:PF01475.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDS00379.1"
                     /translation="MKDAISILRQCDIQPTPQRIAVVEYVLKCKTHPSADDVFTHARK
                     NCPTVSRATVYNTLNLLVEKGLLGMQTIREGAVIFDANVEKHHHFIDDDTGDIYDIPW
                     DQLEVKGKGKLKDFEIFEFQVIMHGRRKKK"
     gene            3058..5250
                     /gene="katG"
                     /locus_tag="ENO07_00025"
     CDS             3058..5250
                     /gene="katG"
                     /locus_tag="ENO07_00025"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007784954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HDS00380.1"
                     /translation="MDRVGKRPVTGMGGGKSNRDWWPNQLNLRILHQHSAKSNPMGAD
                     FNYAEEFKKLDFKALKKDLHALMTDSQEWWPADYGHYGGLFIRMAWHSAGTYRMGDGR
                     GGGGTGNQRFAPLNSWPDNVNLDKARRLLWPIKQKYGKNISWADLMILAGNCALESMG
                     LKTFGFAGGRADIWEPEEDVYWGSEEEWLATSDKPKSRYTGDRELETPLAAVQMGLIY
                     VNPEGPDGNPDPVASGKDVRETFARMAMNDEETVALVAGGHTFGKCHGAGDASLVGPE
                     PEAAPIEEMGLGWKSSFNSGKGADTITSGIEGAWKPNPTKWDMGYLKVLFKYDWELVK
                     SPAGAHQWLAKDVDEEDMVVDAHDPSKKHRPMMTTADLSLKFDPIYEPIARHYLENPE
                     KFADAFARAWFKLTHRDMGPISRYLGPEVPAEELLWQDPVPPVTHELIGDDEIAELKG
                     RILNSGLSIPRLVSTAWASASTFRGSDNRGGANGARIRLEPQRSWKVNQPDQLKKVLE
                     TLEKIQRDFNGSQMGGKQVSLADLIVLGGCAAVEKAAKDAGHDVKVPFTPGRTDATQE
                     QTEIESFAALEPTADGFRNYLADEHRLAAEHSLVDRAQLLTLTAPEMTVLVGGMRVLN
                     ANFGQSQHGVLTRRPESLTNDFFVNLLDMSTEWKATSKDEEVFDGCDRSTGEKKWTGT
                     RVDLIFGSNSQLRAIAEVYASDDSQEAFVRDFVAAWSKVMNLDRFDLD"
     gene            complement(5627..>6066)
                     /locus_tag="ENO07_00030"
     CDS             complement(5627..>6066)
                     /locus_tag="ENO07_00030"
                     /inference="COORDINATES: protein motif:HMM:PF13602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HDS00381.1"
                     /translation="VCSGRNVEMVRSIGADHVIDYTKEEFWRSEKKFDLIVDNAAFQS
                     IGKPLRVLKATGIYVGVGGSSSTLSFLQSLIVNPLIARMKGRKVVSFMANVKQADLVS
                     IKELLEAGKVVPVIDRKYALSETPEAIRYVEKGHARGKVIITI"
BASE COUNT         1520 a         1500 c         1592 g         1454 t
ORIGIN      
        1 cacctttact cttcttatcg gcagcatcgc cggaatttcc ctgctcgttg gcggaatcgg
       61 aattctggcc ataatgctca tcgccatcag agaaagaacc aacgaaatcg ggctcagaat
      121 ggccgtcggc gccagtcgca aagatatcat gacgcaattc ataatggaag catccattct
      181 gggagtgagc ggcggactcg tgggaattgt cctcggtatt ttgggctcta ttctggtgag
      241 cgcgggaaca tcatgggcaa taagcatttc gtcggaatcc gttttgcttg ccttcggctg
      301 ttcattgctg gcggggcttt tcttcggggt atatccggcg caccgtgcct cgatgctcga
      361 tccgattgat gcactgcgga gcgagtgacg accgcggccg gctgtaatat ggcgggagtt
      421 tcgcatgcgg tctgtttgga atcaaatcgg gaatctacta cttagcctga caattatctg
      481 gatcgccgga atccgcactt ctgctaagcg cgtcccccaa ggcctgataa cgttgctgta
      541 catcctgttt atcatccttt tcggatataa actcttcact cgctcgcaga atcatattcc
      601 cctgcctcaa gagggcttcc cgctgttctg catttctgca aacgcctgca atcatagtga
      661 gcgtctccag gagccgagtc gtcaccgcca catttgattg actgttctga cgaatctgat
      721 cgaaagcagc attgacgaca cctgtaaagg tccatggttt ggttaccaga cggagtttgc
      781 cctcatcttc gcagtgatgt gatgacggga atgatcggcc cgcaaggaag gatactgccg
      841 agccaagacg gtctatgcag gaaatcgctg taaatggatc gttgattccg ggtgaaagtg
      901 cccgcacggc gatttccacc agttgatgga tggcatactc tgcatcttgc agtggggatc
      961 gctgggggcc gacaaaaaac gagttggcaa gatcctctgc agattcgtcg ctgaattgac
     1021 cgtcgaagct cacagttaca agtgtgctat cggccgtgat gtattccccc ggacgatact
     1081 caagagagag aagtgaatcc gtggcgatgg ctatacgcag caggccgtta atgtccacag
     1141 cctgcagata gccgtctttc ggagcagaga ttgatcgcga tttgcggtac gagtttatcc
     1201 gggcctgacg gtcctgatcc aattcaccgg tcgatcctgt gtcatccgca atctcgtcag
     1261 gaaacaaggg caataagcgc tccagcagtt cattgtaaac agcctggaca acgttatcag
     1321 cttgtattga tgcggaaacg tgatggataa agtaaacgag aatacccaca ttggcgataa
     1381 ccagcaagat tgaaagattc accgacagga tcggaacgaa tagatatccc tcttgatcgt
     1441 gtattgatcc gagcaccagg aggcagtaaa taaaagtgcc cacaaaggcg ccgagtacaa
     1501 cctggttgcc agggtgtctg acaaagttgc gcagcagacg cgggccaaat tgagatgttg
     1561 tgagagtcag ggcaacaatt gttatcgaaa acgtaacacc ggcgaccgta atcatcgagc
     1621 cggcaatggt cgctaaaatc gaacgagcgc cgtccactcc cccggagtaa aagaaactaa
     1681 ggaatccata ggaatcaggc tcgaactgcc tgtcaaaata caccatgata atcgctaaac
     1741 ctgttgcgat agcgaccatc aagccgggaa taaaatagaa actggttctt atggattccc
     1801 agacattaag aagctttgtc ttcaaaggtt accaatatct gccatactgc tctagtctat
     1861 tgcagccatg aattaatttt ttcaactgcc gcctcggaaa tattatccca cccatcttta
     1921 atcagctcat tgagctcatt gagttggttt tccagtttct cgatttcatc attctttttt
     1981 ccatgagcct cagctttgag ctgggtcaga ttttgctcga gctctttctt cttcgcctcg
     2041 acgcgtgcct tgagttcatt tgttctatct ggcataattc tctccttaaa atttaattag
     2101 acaggccctt cttaaaaaat acgatttttg tggacagagg tcaactgttt tcggcaatgg
     2161 ctgccggcga tggtaaggca ccgcccagaa tctgaaaatt cggtacagtt cgaactgcct
     2221 ggccgaggat tcctgaaatt ttttgactaa tagaagtgat atataaagaa agacggccca
     2281 atagcaccct caccggactt cagtgaattt agttttcgca ttcaaacggg ccattccaat
     2341 tcaaacggaa accattggaa gacaatcaat tctgccggtg aggacaagtc tggtggattt
     2401 gaaagcaatg agaaatcgat ttctccctcc gctcttctca tatttcgggg ttatgcagca
     2461 gtattctttg catttatttt tgcaactttc accttgacat tgtctaaaat actaattatt
     2521 ctaattcgat gaaagatgct atctcgatcc tgcggcagtg tgatattcaa cccactcctc
     2581 agagaattgc ggttgttgaa tatgtattga aatgcaaaac acatccctct gcggatgatg
     2641 tctttaccca tgctcgaaaa aattgcccca cagtttctcg agccaccgta tacaatacat
     2701 tgaacctgct tgtcgaaaaa gggcttcttg ggatgcagac gattagggaa ggtgcggtta
     2761 tctttgatgc caatgtcgaa aagcatcatc atttcatcga tgatgatacc ggtgatatat
     2821 atgatatccc ctgggatcaa ctggaggtca agggaaaggg aaagcttaag gatttcgaga
     2881 tttttgagtt tcaagttata atgcacggaa gacggaaaaa gaaatgattc tttttttggc
     2941 gtgtaataat acattgtcta aatttagact atgttgcacg agcgacagac aatcttacct
     3001 atgaacacaa gtagagccaa ttacactatg taaggagcgt ttatgagtga ccaaaacatg
     3061 gatagggtgg gcaagcgccc ggtgacaggc atgggcggcg gcaaatccaa ccgggattgg
     3121 tggccgaatc aactgaatct gagaattctt catcagcact ctgccaaatc caatcccatg
     3181 ggtgcggatt tcaactacgc tgaggaattc aagaaactcg actttaaggc gttgaagaaa
     3241 gacctccatg ctttgatgac tgactcgcaa gagtggtggc cggctgatta tggtcactac
     3301 ggtgggcttt tcattcggat ggcgtggcat agcgctggaa cctatcgcat gggcgatggc
     3361 cgtgggggcg gcgggaccgg aaaccagcgt ttcgcacccc tcaacagctg gccagacaat
     3421 gtaaatctcg acaaagcacg tcgcctgctc tggccgatca agcagaaata cggcaagaat
     3481 atctcctggg cagatctgat gattcttgcc ggcaactgcg cacttgaatc gatgggattg
     3541 aagaccttcg gtttcgccgg cggtcgtgcg gatatctggg agccggaaga ggacgtttac
     3601 tggggttccg aagaggaatg gctggcgacg agcgacaagc ccaagagccg ttacacgggc
     3661 gaccgggaac tggaaacccc cctggcggcc gtgcaaatgg ggctgatcta tgtgaatcca
     3721 gagggcccgg acggcaaccc ggatccggtc gcttcgggca aggacgttcg ggaaacgttt
     3781 gcccgaatgg cgatgaacga cgaggagacc gttgcgcttg tagctggcgg gcacacgttt
     3841 gggaagtgtc acggcgcggg cgatgcatcg cttgtcggtc cggaacctga ggcggccccc
     3901 attgaagaga tgggcttggg ctggaagagc agctttaaca gcggcaaagg cgctgatacg
     3961 atcaccagcg gcatcgaggg cgcctggaag ccgaacccga ctaaatggga catgggctat
     4021 ctgaaggtgc tgttcaaata tgactgggag ctggttaaaa gtccggctgg tgcgcatcaa
     4081 tggctggcta aggacgtgga tgaagaggac atggttgttg acgcacacga cccgtcaaaa
     4141 aaacaccggc cgatgatgac cacggcggac ctctcgttga agttcgatcc catctacgag
     4201 ccgatcgcgc ggcattatct ggagaatcct gagaagttcg cggatgcatt tgcgcgggcc
     4261 tggttcaagc tgacacatcg tgacatgggg cccatctcgc gctacctggg accggaagtt
     4321 cctgccgaag agctgctctg gcaggacccg gtgcctcctg tcacccacga gctgattggc
     4381 gacgacgaga tcgctgaact caagggcagg atcctcaatt cgggattgtc cattccccgg
     4441 ttggtatcga ctgcatgggc atcggcctcg acattccgcg gcagtgacaa tcgtggcgga
     4501 gcgaacgggg cacgtattcg tctcgaaccg cagcgcagct ggaaggtgaa ccagcctgac
     4561 cagctcaaga aggtactgga aactttggaa aaaatccaga gggactttaa tggctcgcag
     4621 atgggaggga agcaggtttc acttgccgac ctcattgtcc tgggcggatg tgcagcggtc
     4681 gagaaggctg cgaaggatgc cgggcatgac gtgaaggttc cattcacacc gggacgtacc
     4741 gacgcaacgc aggagcaaac cgaaatcgag tcctttgccg cgctcgagcc gaccgcagac
     4801 gggttccgca actatctggc cgacgaacat cgactggcgg cagaacattc attggtggat
     4861 cgtgctcagt tgctgacatt gactgcgcct gagatgacgg ttcttgtggg tggcatgcgc
     4921 gtcctgaatg caaatttcgg tcagtcacag catggtgttc tgaccaggcg gccggagtcg
     4981 ctcacaaatg acttcttcgt caacctgctc gacatgagca cggaatggaa ggcaacgtcg
     5041 aaggacgaag aggtgttcga tggatgcgac cgttcgaccg gggagaagaa gtggaccggc
     5101 acacgtgtgg acctcatctt cggttcgaac tctcagctcc gagccatcgc ggaggtttac
     5161 gcgagtgacg actcgcagga ggcattcgta cgtgacttcg tagctgcatg gagcaaggtc
     5221 atgaatctcg atcgctttga tctcgactaa agcagcgaac agcttggata gtgcagataa
     5281 tgctaataac cgggaggtcg atcacatcga ccttccggga ttatgtcaat gatgtacgat
     5341 gcctgcctga accatttcaa catgaaccgc aagcgattcc ggacttgctg cctccaggta
     5401 ttgtacagtc gagagaaacc ctatcctgca tacagcaagt ccattcacat acttgcaaaa
     5461 gcgccttaga atacaacaaa gcgcttccga tttcgctgtc gccaatctta tgtgagcttc
     5521 gggcttccag tctcgttacg gaatgccatt attgaccgaa gtcgctggaa aacaatcaaa
     5581 tctggtcaat gaagtgcaaa aaaagcgtct ttgctgctat ccatcatcag atagtgatga
     5641 tcacttttcc tcgcgcgtgt cctttttcaa catatcggat tgcctcaggc gtttcactca
     5701 aagcatactt cctgtctata acgggcacga ctttgccagc ttccagaagc tcctttatgg
     5761 agaccaaatc tgcctgcttc acgttcgcca tgaatgaaac tacttttctg cccttcatcc
     5821 ttgcaatcag aggattgaca atcagcgatt gcagaaaact caaggttgat gaggagccgc
     5881 caactccgac atatatgccg gttgctttca gcacgcgcag aggctttcct atcgactgaa
     5941 atgcggcatt gtctacgatc agatcaaatt tcttctcgct tcgccagaat tcttcctttg
     6001 tgtaatcaat cacatggtcc gcaccgatag accgcaccat ctctacgttc cgcccactgc
     6061 agacac
//
LOCUS       DSEB01000002            2867 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004541, whole genome shotgun sequence.
ACCESSION   DSEB01000002 DSEB01000000
VERSION     DSEB01000002.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004541"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(21..416)
                     /locus_tag="ENO07_00035"
     CDS             complement(21..416)
                     /locus_tag="ENO07_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00382.1"
                     /translation="MGKCNNLIVHCMDYRLQDTIDDWIKERGLNGKIDRISIGGPCKD
                     IEFAMKYIKTCIEKHGVKSVYLTQHEDCAGYGGHDAFTSLDAERERLTDDMMDLKANI
                     KLLYPKVEVHTILMREDDEGWDMVDMGKD"
     gene            complement(563..1090)
                     /locus_tag="ENO07_00040"
     CDS             complement(563..1090)
                     /locus_tag="ENO07_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00383.1"
                     /translation="MMSHRIKIILFAAILLLTGCSGEIGLNSDQRLRGQWYAEFVGTY
                     FSMTVTPEGGENQYTLNHESRLGFSDGYFEVFIEPPVPPLFGNGESYSCFSCLYSISG
                     DTLIFSDQSTGDEIERYSFNVSGDTLSLSYLPLVIAEGDGETIAAVPLYGGLPWGRSR
                     MWHAGEFFRLPHTAE"
     gene            complement(1181..2083)
                     /locus_tag="ENO07_00045"
     CDS             complement(1181..2083)
                     /locus_tag="ENO07_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDS00384.1"
                     /translation="MMMNGRSPRVRIVQRVTAWGMVANLGLAGLKFAAGFLGNSKALV
                     ADAVHSITDTTTDIAILIGAPRWSAPADEDHPHGHGRIETLITAFIGLVLIAAAVGLG
                     YNAVSTLQEARDTGPGWIAFGAALASILVKELLYQWNARVGRRVKSSAMVANAWHHRS
                     DALSSLPVAVAVLGTKIQPSWTFLDHVGALVVSLFILQAAWKIFWPALKQLTDVGASK
                     EELEELYDMIKHIDGVRDVHALRTRNIGPGLQVDLHLQVDPELSVRDGHDLAGIAKHR
                     LLEHENVVDVLIHIEPFEKQDNDS"
     gene            complement(2230..2301)
                     /locus_tag="ENO07_00050"
     tRNA            complement(2230..2301)
                     /locus_tag="ENO07_00050"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(2266..2268),aa:Arg,seq:ccg)
     gene            complement(2360..2809)
                     /locus_tag="ENO07_00055"
     CDS             complement(2360..2809)
                     /locus_tag="ENO07_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HDS00385.1"
                     /translation="MRITSPAFVQGGTIPARYTCDGDDICPELFWKNVPGEAVSLAMI
                     VDDPDAPGGTWVHCVAYNISPADTGISTEEIAAGQEIVLGKNSWGKNDYGGPCPPSGT
                     HRYFFKIYVLDKKLDLKPGATKEEVMNAMEGHILARGELMGTYHRQG"
BASE COUNT          698 a          773 c          669 g          727 t
ORIGIN      
        1 ctgttacccg ttcgcaggta tcagtctttg cccatatcaa ccatatccca gccctcatcg
       61 tcctcacgca tcaaaatcgt atgcacctca actttcggat aaagcagctt aatattggcc
      121 ttcagatcca tcatatcgtc tgttaagcgc tctctttcgg catccaatga ggtaaaggcg
      181 tcatgcccgc catatccggc gcagtcctca tgctgggtca aataaacact tttcacgcca
      241 tgtttctcga tacaggtctt gatgtacttc attgcgaatt cgatatcctt gcagggtccc
      301 cctattgaga tccggtcgat ttttccgttc agcccccttt ccttgatcca gtcgtcaata
      361 gtatcttgta gacgataatc catgcagtgt acgatcaggt tgttgcactt tcccatattg
      421 tcctcctcca taccttggat gatgggatga tattataata taatccggca aagttcaagt
      481 attatctgca cacaatctga ctaattgtgc agattgcccc ggctgtagaa gggtagtctt
      541 tgaggattaa aaatggatct gtttattcag cggtgtgcgg cagtcgaaaa aattccccgg
      601 catgccacat gcgggaccgt ccccatggca aaccgccgta gagcggaaca gcagcgatcg
      661 tctcgccgtc accttcagca attacaagcg gcaagtagga taaactgagc gtatcccccg
      721 atacattgaa tgaatatctt tctatctcat cgccagtgga ttgatcggaa aatataagcg
      781 tatcaccgga tatcgaatag aggcaactga agcaggaata tgactccccg ttgccgaaaa
      841 gcggcggaac ggggggctcg ataaagacct caaaatatcc atctgaaaag cccaaccttg
      901 attcatgatt cagggtgtac tgattttcgc cgccttccgg agttacagtc atactgaagt
      961 atgtaccaac gaattcagca taccactgcc cccgaagccg ttgatctgaa ttcaggccaa
     1021 tctcaccgct gcatccggtc agcaacagga tggcagcaaa aagtatgatc ttgattctat
     1081 ggctcatcat aaataaaata ttaaacagca gcgatctgtc aataatgatc cacaatattt
     1141 gtgtattata atctgttcag tggaaaccgg aatattctta ttagctgtca ttatcctgtt
     1201 tttcaaaggg ttcgatatga atcaggacat cgacgacgtt ttcatgctca agcagcctgt
     1261 gctttgcgat tcccgccaga tcgtggccgt cccgaacaga caattccgga tcgacctgca
     1321 ggtgcagatc aacctgcagc ccggggccga tatttcttgt gcgcagggca tgaacatcgc
     1381 gcacaccgtc aatatgcttg atcatatcat ataactcttc cagctcctct ttggaggcgc
     1441 ccacatcggt aagttgcttt agagcgggcc agaatatttt ccaggccgcc tgtaaaatga
     1501 acagcgatac caccagcgcg ccgacatgat ccagaaatgt ccaggatggt tgaatttttg
     1561 tacccagaac cgcgactgct accggcaacg agcttaaggc atcgctcctg tgatgccagg
     1621 cattagcaac catggccgag gattttacac gccgccctac acgggcattc cactggtaaa
     1681 gcagctcctt caccagtatc gaagccagcg ccgctccgaa ggctatccat cccggtcctg
     1741 tgtcccgggc ctcttgcaga gtcgatacag cattgtaacc cagaccgacc gccgcagcta
     1801 tcaggacaag gccaataaat gcggtgatca aagtctctat gcggccatgg ccgtggggat
     1861 gatcctcgtc ggcgggtgcg ctccagcgag gggcgccgat caatattgcg atatcggtgg
     1921 tggtgtctgt gatgctgtgg acggcatcag ccaccagggc tttgctgtta cccagaaacc
     1981 cggcggcgaa tttcaaacct gccagcccga gatttgccac catgccccag gcggttacac
     2041 gctgcacaat gcgcacacgc ggcgaccttc cgttcatcat cattattcta cccttatttc
     2101 tatgcataaa taataaggcc tgttaaattg aacaactcag gccaatatag caattttttt
     2161 atatgatttt caatgattat atgaagattg gcagcggtat cctcagacct catcgatcgc
     2221 acaaaaaaat acccccgccc aggatcgaac tgggattgcc ggctccggag gccggtgcgt
     2281 tatccattac accacggggg caattacgcc attatactat tatttatggc tgtggttgtc
     2341 aatcaaaaac agaagggcgt catccctgcc gatgataggt tcccatcaat tcgccccggg
     2401 ccaggatgtg accttccatg gcattcatga cttcctcttt agtcgcaccc ggttttaaat
     2461 cgagcttttt gtcgaggacg tatattttga agaaatagcg atgcgttccg ctgggagggc
     2521 agggcccgcc atagtcgttt ttgccccaac tgttctttcc caatacaatc tcctggcccg
     2581 cagcaatttc ttcggtggaa atgcccgtgt cagcggggga gatgttgtag gccacacagt
     2641 gcacccaggt tccgcccgga gcatcgggat cgtcaacgat catggccagc gaaactgcct
     2701 cgccgggcac attcttccaa aacagctcgg ggcagatatc atctccgtca caggtgtagc
     2761 gggcaggaat tgtgcccccc tgtacgaagg ccgggctggt tattctgatt tcaggcatgg
     2821 agtcctctgt gacttcttgt gtctccgcca tggctgttgt ttccggc
//
LOCUS       DSEB01000003            4903 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001386, whole genome shotgun sequence.
ACCESSION   DSEB01000003 DSEB01000000
VERSION     DSEB01000003.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001386"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..364)
                     /locus_tag="ENO07_00060"
     CDS             complement(<1..364)
                     /locus_tag="ENO07_00060"
                     /inference="COORDINATES: protein motif:HMM:PF01180.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDS00386.1"
                     /translation="MSIANLSIEICGIRFRNPILTASGTFGYGEEFNQIIDLSKLGGI
                     VTKSITKHPRPGHPPPRTHETAAGMLNAIGLANVGVDKFIEEKIPFLKTLDTRIIVNV
                     AGFSIEEYTYTVERLNDTD"
     gene            complement(361..1209)
                     /locus_tag="ENO07_00065"
     CDS             complement(361..1209)
                     /locus_tag="ENO07_00065"
                     /inference="COORDINATES: protein motif:HMM:PF10418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDS00387.1"
                     /translation="MAVLQNKGGAAARDQGTTMIFNPGVRVVEKKQLLPDLFHLKFEG
                     ESLASRAVPANFVQIKVSKGLDPIFRRPMSIHYADGKTFEMVFRTVGRGTHILSQAEI
                     DQEFDCIGPLGNSFKLPEDGERAVMVAGGVGFPPLYFFSRFLVEKHNFPKENILFLLG
                     MKTVSEKPMADDLLKLDIPLEFSTDDGSFGFHGFVTDLFEAHYAKKLRHEKIRIYSCG
                     PNAMMRRLGQIALSFKVPCQLSLEGAMPCGVGTCLGCAVKMRGRNDYYRVCQEGPVFD
                     AEEVEL"
     gene            complement(1152..3290)
                     /locus_tag="ENO07_00070"
     CDS             complement(1152..3290)
                     /locus_tag="ENO07_00070"
                     /inference="COORDINATES: protein
                     motif:HMM:PF13174.4,HMM:PF13432.4,HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HDS00388.1"
                     /translation="MVGSKMRFFKITAIALAIMLLFSGCVYFNTFYLAKKNFNEAESK
                     REKQGSEEAVGGSSAQYQAAIEKSLTVITDHPDSKYVDDALFIIGKSYYHLGDFSKAE
                     RKFRELLAAYPESDYYDRALFYLGKCRLKQEEYILARQSFIQVDSVTKNRKWKAEAQY
                     MIGEIEFNERNFEQAVKYFSSYLEKFADASMAAQVQFKIGQAYDSLENYEASKDAYLN
                     VADYGPSDSLYFESQFEAGESYYAIDMIDSGYAIFEKLSKDEKYYSSAGMIRLKLAEA
                     LEMKGELEAAIEEYAKITEEFQRSDPAARAFYRMGEIYMLELSDLETAKAMYDSSITA
                     NRRSEIYQMALERSTNISKLSQYRQEASAEDLESAARSQFQLAELYVFELENPDTALH
                     EFQVVIDSFPESEYAPKALLARGYIFDKHLGMQDSSRAEYRKIIEKYPTSDQVEEAAK
                     ELNINLESIDTDFAGQRYKNAEKLIFNEGNVDSALVILQSIIDEFPNSRYAPKAAYAK
                     AWLTEKHVAPDEWDPNDSTSVPDSTIIIAYQNVADQYAGTPYGDSALVKLGKKVVPKP
                     KVPQQQQDTIPQDTLLAGDQDEIEDDRDMVIIGQDTLIANEIATLIDTLHVVDGDPIK
                     EGDFVYPPSAYYSRFEGYVGFMVRIDFLGKPTDWRILQPSGIKEIDEAAVEALRYTEF
                     DVGQIPPEYIEQWQFYRIKVELPPEIKGRQ"
     gene            complement(3262..4152)
                     /locus_tag="ENO07_00075"
     CDS             complement(3262..4152)
                     /locus_tag="ENO07_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDS00389.1"
                     /translation="MILTHRKNTKILLDRLKDCDLCPRECHVDRTMGETGICGQTDKI
                     KVSSANLHFGEEPPISGFNGSGTIFLTGCNLNCVYCQNYPISQMQNGREVSPLTLVDS
                     MLRLQELGAHNINFVTPTHFNAQIADAIVAARDRGLKIPIVYNSSGYDRVEVLRMMEG
                     LIEIYMPDMRYGDENAAQCYSSAADYPRINRAAVAEMHRQVGDLQTDENEVATRGLLI
                     RHLVLPNNLSRSEEIFKFISEKISPDTYISLMSQYFPAFRANEYPQISRRITKKEYAV
                     AKKLMEKYNLSNGWFQDEIL"
     gene            complement(4164..4898)
                     /locus_tag="ENO07_00080"
     CDS             complement(4164..4898)
                     /locus_tag="ENO07_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HDS00390.1"
                     /translation="MFSETLIELLKKDPKICVLTGAGVSAESGVPTFRGKEGIWKKFR
                     AEELATVDAFLSNPELVLAWYQHRRDIIDNIEPNPGHYALAKMEKHFSDFCLVTQNVD
                     ALHRKAGSEHVLEVHGNIMRNKCLTCGKPVNRLDFKEGDDIPRCECGGMIRPDVVWFG
                     EMLPADVFAEANKKSFQCELFLSIGTSAIVYPAAGLPIAAKQRGAYLVEINVQETELS
                     TYADEVLLGPSGEILPKLLMAAGIDI"
BASE COUNT         1158 a         1263 c         1162 g         1320 t
ORIGIN      
        1 cgtctgtgtc attcagccgt tccactgtat aagtatattc ctcaatagag aaaccggcca
       61 cattaacgat aatgcgcgta tccagagtct tcagaaaggg gattttttcc tcgatgaatt
      121 tatccacgcc cacattggca agaccgatgg cattaagcat acccgctgcg gtttcatgtg
      181 ttcgcggcgg gggatggccg ggtcggggat gtttggttat tgattttgta actatcccgc
      241 ccagtttgga aaggtcgatg atctggttga actcttcgcc gtagccgaat gtgcccgagg
      301 cggtcaaaat cgggtttctg aacctgattc cgcaaatttc gatcgaaaga ttggcaatac
      361 tcataattcc acctcttcgg catcaaaaac cggtccctcc tggcagacac ggtagtaatc
      421 attcctgccg cgcatcttaa cagcgcatcc caggcaggtg ccgaccccgc agggcatcgc
      481 gccctccagg gaaagctggc agggaacttt gaaactgagg gcaatctggc ccagccgtct
      541 catcatcgcg ttggggccgc aggaataaat cctgatcttc tcatgtctca gctttttagc
      601 ataatgggcc tcgaataaat ccgtgacaaa accgtgaaaa ccaaaactcc cgtcatcggt
      661 tgagaactct aagggaatat cgagtttgag cagatcatcg gccattggtt tttctgagac
      721 ggttttcatg ccgagcagaa agaggatatt ctctttcggg aagttatgct tttctaccag
      781 aaacctgctg aaaaaataga gtgggggaaa accgactccg cccgccacca taactgcccg
      841 ttccccatcc tccggcagtt taaagctgtt tccgagagga ccgatacagt cgaattcctg
      901 atctatttca gcttgtgaga ggatatgggt gcccctcccg actgtcctga aaaccatctc
      961 gaaggttttc ccgtcagcgt aatgaatgct catggggcgc cggaatattg gatccagacc
     1021 tttggaaacc ttaatctgca cgaaattggc gggaacagca cgagaagcga ggctttctcc
     1081 ctcgaacttc agatgaaaca ggtcaggaag aagctgtttt ttctctacca ccctcacacc
     1141 gggattaaag atcattgtcg tcccttgatc tcgggcggca gctccacctt tattctgtaa
     1201 aactgccatt gctcaatata ctccggcggt atttgtccaa catcgaattc tgtgtatctt
     1261 aatgcttcga cagccgcttc gtcaatctct tttatccccg agggctgcaa aatccgccaa
     1321 tcggtgggct tgcccagaaa gtcaatcctg accatgaagc cgacataacc ttcgaagcgc
     1381 gagtaatatg cagagggcgg ataaacaaaa tctccctctt ttatcggatc tccatcaacc
     1441 acatgcaggg tgtcaatcaa agtggcgatt tcattggcaa tgagggtgtc ctgcccaatt
     1501 atgaccatat cgcggtcatc ttcaatttca tcctgatccc ccgccaataa tgtgtcctgg
     1561 ggaattgtgt cctgctgctg ctgagggacc ttgggcttgg gaaccacctt tttgcccagt
     1621 ttgaccaatg cggaatcccc gtatggtgtg ccggcatatt gatcggccac attctgatag
     1681 gcgatgataa tagtcgaatc aggcacagaa gtcgagtcat tcggatccca ttcatccggt
     1741 gcaacatgct tctcggtcag ccaggctttg gcataagccg ctttgggagc atatctggag
     1801 ttggggaact catcgattat gctctgcagg atgaccaggg ccgaatcgac attgccctca
     1861 ttgaatataa gcttctctgc gtttttgtat ctctgcccgg caaaatcggt atcaatgctt
     1921 tccagattta tattcagctc cttggcggct tcctccacct ggtcactggt gggatatttt
     1981 tcaattattt ttctgtattc ggcacgggag ctgtcctgca tacccagatg cttgtcgaag
     2041 atatagcctc gcgccagaag tgccttaggg gcatattcac tttcagggaa gctgtcgatt
     2101 acgacctgga actcatgcag ggcggtatcc gggttctcga gctcgaagac atacagctcc
     2161 gccagttgaa actggcttcg ggcagcactt tccaggtctt ccgccgatgc ttcctggcgg
     2221 tactgagaga gcttgctgat attagtgctt ctttctaatg ccatctgata tatctcagaa
     2281 cggcggttgg cggttatgga ggaatcatac atcgcctttg cagtttccag gtcgctgagc
     2341 tcgagcatgt aaatctcgcc cattctgtaa aaggcgcggg ccgcaggatc agacctttga
     2401 aactcctctg tgattttggc atattcctcg atcgccgctt ccagctcgcc cttcatctcc
     2461 agcgcttccg ccagcttgag cctgatcatc ccggccgagc tgtagtattt ttcatctttg
     2521 gataattttt caaatatcgc gtagcccgaa tcaatcatgt cgatagcata ataagattct
     2581 cccgcttcaa actgcgattc aaaatacagg ctgtctgaag gaccataatc ggccacattc
     2641 aggtaggcgt cttttgatgc ttcatagttt tccagggagt catatgcctg gccgattttg
     2701 aattgcacct gggctgccat gctggcgtca gcaaattttt caagataact tgaaaagtat
     2761 ttcacggcct gttcgaaatt gcgctcattg aattcaatct cgccaatcat gtactgcgcc
     2821 tcggccttcc atttcctgtt ttttgtcacc gaatcgactt gtatgaatga ctggcgggcg
     2881 aggatatatt cttcctgttt aaggcggcat ttgcccaggt agaagagggc ccggtcataa
     2941 taatccgatt cgggataggc tgccagtaat tcacggaatt tgcgttccgc cttggagaaa
     3001 tcccccaggt gatagtagga tttgccgata atgaaaagtg catcatcgac atatttgctg
     3061 tccggatgat cggttattac cgtcagtgat ttctctattg ccgcctggta ctgtgcggac
     3121 gatccgccga ccgcctcctc ggagccctgc ttttcccgtt tactctcggc ctcattgaaa
     3181 ttcttcttgg ccagatagaa agtgttgaaa taaacacatc ccgagaacag gagcattatc
     3241 gctaatgcta ttgctgtaat cttaaagaat ctcatcttgg aaccaaccat tgcttagatt
     3301 atacttttcc attagctttt tggcaacagc atactccttt ttagttatac gtctcgaaat
     3361 ctgcggatat tcattagccc tgaaggccgg aaaatactgc gacatcagtg agatatacgt
     3421 atcgggcgat attttttctg aaataaactt gaatatctcc tccgagcggg agagattatt
     3481 gggaagcaca agatgcctga tcaggagccc ccgggtggcg acttcgtttt cgtcagtctg
     3541 caaatctcct acctgccggt gcatctcagc aacagccgcg cggttgatcc ggggataatc
     3601 agcggcggac gaatagcatt gagccgcatt ttcatccccg taccgcatat ccggcatgta
     3661 tatctctatc aggccctcca tcatacgcaa aacttcgacc ctgtcatatc ccgaagaatt
     3721 atagactatc ggtatcttca atcctcgatc tctggcagca acgatggcat ctgctatttg
     3781 cgcattgaaa tgggtggggg tcacgaaatt gatattatgc gctccaagtt cctgcagccg
     3841 gagcattgag tcgaccaggg tcagagggga tacctcacgc ccgttctgca tctggctgat
     3901 cggataattc tggcagtaga cgcaattcaa attacagccg gtcaggaaga tagtcccgct
     3961 gccgttaaag ccggatatgg gcggttcctc gccgaagtgc agattggccg aagaaacctt
     4021 aattttgtcg gtctgcccgc atatgcccgt ttcgcccatg gtcctgtcca catgacattc
     4081 acgaggacat agatcacagt ctttcaggcg atcgagcagt atttttgtat ttttccgatg
     4141 agtcaagatc atggttttca aaattatatg tcgattccgg cggccattag gagtttagga
     4201 aggatttctc ccgaaggtcc cagcagaacc tcatcagcat aagtggaaag ctccgtttcc
     4261 tggacattaa tttctaccag ataagctccc ctctgtttgg cggcaatcgg aaggcccgca
     4321 gcgggataga ctatggccga ggttccgatc gataaaaaca gctcacactg gaaagatttt
     4381 ttgttggcct cggcaaaaac atccgccggc agcatctctc caaaccagac tacatcggga
     4441 cgaatcatcc cgccgcactc acatctgggg atatcatcac cctccttaaa atccagcctg
     4501 tttacaggct taccgcaggt caggcatttg ttgcgcatta tgttgccatg cacctcgagc
     4561 acatgctcgg agccggcttt tctgtgaagg gcgtcgacat tctgggtaac caggcagaag
     4621 tcggagaaat gcttttccat tttcgccagt gcgtaatgcc ccgggttggg ttcaatatta
     4681 tcgattatat cccgcctgtg ctgataccat gccaggacaa gctccggatt tgagagaaaa
     4741 gcgtcaactg tggcaagttc ctcggccctg aactttttcc agattccctc tttgccgcga
     4801 aatgtgggca caccggattc agccgagacc cccgctccgg tgagtacaca gattttggga
     4861 tcttttttca gcagttcaat taaagtctct gaaaacatag cag
//
LOCUS       DSEB01000004            4911 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001381, whole genome shotgun sequence.
ACCESSION   DSEB01000004 DSEB01000000
VERSION     DSEB01000004.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4911)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001381"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1330
                     /locus_tag="ENO07_00085"
     CDS             <1..1330
                     /locus_tag="ENO07_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDS00391.1"
                     /translation="NITMVLGSDYMGEDKKGFLRNGMFAAGALDMLGLHAGTKVVVIR
                     DAKTNKLKKFGVFLFGLSATGKSTWSCHQLGLDWKKGERTLVCQDDICFLKNDGSAYG
                     SEQNYFVKTDVDKKLQESMYNALIHKSAMYENVMIDSEGNPQFLDENLCGNGRAVIMR
                     KQLKIRRGHWPFAMKNIWYPSLNLPPVDELDGIVFAFITRRNTIMTFSQRLNPTQAAL
                     AYLWGESSHSYASNPAKAGESVRTVGTDPFIVGSRAEKVNKFYKIVMDLADSYPDKVH
                     FFQYNTGGMGEIIEKDKNGNKKMIRKTERVPIDTMAAIQRGDLRGTNEYRPSFLGTEE
                     ILSAEGVDLSMYRPEKYYSREQIDNYVRDLVNGRRKFTEQVAEEGLMPEIIRAAEESF
                     SIAPDKAAKVSMASIRKREEPPPPKEKPAAKLTDWKPNPRLARDRGWRYG"
     gene            1539..1934
                     /locus_tag="ENO07_00090"
     CDS             1539..1934
                     /locus_tag="ENO07_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDS00392.1"
                     /translation="MMTKFEFTVEGTPISQQAKKRRILNQWKAQVRRTARKYWPHNQP
                     PAKDQLRITLTYFYKFEALDVDNMIKPIQDSLIGLVYHDDRQITDVYSRKRKLNTSFR
                     LLNVSPAIARGLASGREFLHILIEDAKMV"
     gene            1941..2600
                     /locus_tag="ENO07_00095"
     CDS             1941..2600
                     /locus_tag="ENO07_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00393.1"
                     /translation="MAQHKTDMERKRITAIAREYAEKGYDVNVSPNRDELPDFLQNYR
                     PDIIATSYNENVVVEVKSGRNLVKSPYLPKLAKAIEENEGWRFEMVITNPRGKKEPID
                     DDIDVLTREDIIKRLEEIKHLLEIGQIEAAILLGWSAIEGTLRLMAMRERIKIKRRTA
                     SYLAKKMYNLGLITPDEYNILNEALELRNAVIHGYKTEKADSAVVEDMLTIIRELLGS
                     I"
     gene            complement(2985..3902)
                     /locus_tag="ENO07_00100"
     CDS             complement(2985..3902)
                     /locus_tag="ENO07_00100"
                     /inference="COORDINATES: protein motif:HMM:PF00583.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDS00394.1"
                     /translation="MKLSIRKIKPDDTRLAERLVMRTMNSLRETHSMSPVPYKPGRKR
                     NTMMCHLLKTDPGGTIGAFSGNRLVGYASAMIRDGHWYLAHLFTDQRFQGKGIGRKLL
                     KRVYNFNKEAEIHTRSLATFAFNPFAVQLYARFGMYPIQLLPMMTWKYDQKKRVQKIK
                     TDYDLKAVPIDDYDQIEILNSFDLPNRGVCRPEDHKFWIDSELVHGFIYQYRGNPVGY
                     TWITRDTVMAPVTSSKPEYLIPCLAETINYMRGRKFEKLIVWVPGSNGSVLDFLLKNK
                     FKLEENELLMSDRPFARDDCYLPASLAFY"
     gene            4149..4664
                     /locus_tag="ENO07_00105"
     CDS             4149..4664
                     /locus_tag="ENO07_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDS00395.1"
                     /translation="MLNRNVLILNQNYEPLTVCSVKRAVVMMLLGKVEMIETMDGHML
                     HSVSAAIRVPSVVRLGFYVKVPRKRIFLSRKNIIKRDGHRCMYCGRSEGSMTVDHVVP
                     KRMGGRDTWENLVCACTKCNNRKGDRTPEQANMTLIKRPKKPNHLMFIQRFIGISDDS
                     WKPYLFMEDFH"
     gene            4665..>4911
                     /locus_tag="ENO07_00110"
     CDS             4665..>4911
                     /locus_tag="ENO07_00110"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:PF00579.23"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDS00396.1"
                     /translation="MAQQVILSGMQPTGSLHLGNYEGALKNWISLQDDYEMYCCIVDW
                     HSLTSLYDKTEVLKDQIFQMAVDYVAAGLDPEKCAIFV"
BASE COUNT         1306 a         1129 c         1277 g         1199 t
ORIGIN      
        1 caatatcacc atggtgcttg gatcggacta catgggcgag gacaagaagg gtttcctgcg
       61 caacggcatg tttgccgccg gggcgctgga catgctcggc ctgcatgccg gaaccaaggt
      121 cgtggtcatc agggatgcca aaaccaacaa gctgaaaaaa ttcggcgtct tcctgttcgg
      181 gctatcggca acgggcaaaa gcacctggtc atgccatcag ctcggcctcg actggaagaa
      241 gggcgaacgc actctggtct gccaggatga tatctgcttt cttaaaaatg acggttctgc
      301 ctacggttcc gagcagaact actttgtcaa gaccgatgtc gataagaagc tgcaggaatc
      361 gatgtataac gcgttgatcc ataagagcgc gatgtatgaa aatgtaatga tcgattctga
      421 gggcaatcct cagtttctgg atgagaacct gtgcggtaac ggccgtgctg tcattatgcg
      481 caagcagctg aaaataaggc gcggtcactg gcccttcgcc atgaagaata tctggtatcc
      541 gtcattgaat cttccgcctg ttgatgagct ggacggtatc gtatttgcat tcatcaccag
      601 gcgcaacacc atcatgactt tctcgcagcg tttaaatccg acccaggccg cactggcata
      661 tctctggggc gaatcgagcc acagctatgc ttccaatccg gccaaggcgg gagagtcggt
      721 gcgcacagtc gggactgatc cattcatagt cggatcgcgg gccgagaaag taaacaagtt
      781 ttataaaatc gtgatggatc tggccgacag ttatcccgat aaagtacatt tcttccagta
      841 taacaccggc ggtatggggg agataatcga gaaagacaaa aacggcaaca aaaagatgat
      901 ccgcaagacc gaacgtgttc cgatcgatac catggcggcg attcaacgcg gcgatctgcg
      961 cggtaccaat gagtatcgtc ccagctttct cggtactgag gaaattctct ccgccgaggg
     1021 tgtggacctg agcatgtatc ggcccgaaaa atattactcc cgggagcaga tcgacaatta
     1081 tgtgcgtgat cttgttaacg gtcgccgcaa gtttaccgag caggtggcag aggagggtct
     1141 gatgccggag ataatcaggg ccgccgagga atcattcagc atcgcccccg acaaggctgc
     1201 gaaagtctcg atggcgtcaa tccggaagag agaggaaccg cctccaccga aggagaagcc
     1261 tgccgcaaaa ttgaccgact ggaagccgaa tccaaggctg gcgcgtgacc gcggctggcg
     1321 ctacggttga ttttttggat ggcaatagga gcgagaattg catttgggat ataatagccg
     1381 cctgaaatac gaggcggttt tttgtgagac cggattgcta attgaatttt tttgaatggt
     1441 gatattttcc aggtcaaaag tcttcgactt tcgacattgt caaatcttta aaagatttga
     1501 cctgctaatt ttcaaagatt caaaatgcaa tagaataaat gatgacgaaa ttcgaattta
     1561 cagtcgaggg aacgccgatc tcacagcagg ccaagaaacg gcggatactg aatcaatgga
     1621 aagcgcaggt caggcggaca gcaagaaaat actggccgca taatcagccg cccgccaaag
     1681 accagctgcg catcaccctg acatatttct ataaattcga ggctctggat gttgacaaca
     1741 tgatcaaacc tattcaggac tccctcatcg ggctggttta tcatgatgac cggcagatta
     1801 ccgatgtcta cagccgcaag cgcaagctca atacaagttt ccgtcttctc aatgtctcgc
     1861 ctgcgatcgc gcgggggctg gccagcggac gggagttcct gcatatattg attgaagatg
     1921 cgaagatggt gtaaagaatt atggcacagc acaaaacaga tatggaacga aaaaggatta
     1981 ctgcgatcgc gcgcgagtat gccgagaagg gctatgatgt caatgtcagc cccaatcgtg
     2041 atgaactgcc tgatttcctt cagaattacc gccctgatat cattgccaca agttataacg
     2101 agaatgttgt ggttgaggtc aagagcggac gcaacctggt caagtcgccc tatcttccca
     2161 agctggccaa ggcgattgaa gaaaatgagg gctggcgttt tgagatggtg attaccaatc
     2221 ctcgcgggaa gaaggaacca attgacgatg atatcgatgt cctgaccaga gaagacatca
     2281 taaagcgcct tgaggaaata aagcatcttc tggagatagg ccagatcgag gcggccattc
     2341 tcctcggatg gtcggctatt gagggcacac tgcggctgat ggcaatgcgt gagcgaatca
     2401 agatcaagcg ccgcaccgca tcatacctgg caaaaaaaat gtacaacctg ggcctcatca
     2461 ctcctgacga atacaatatc ctgaatgaag ccctcgaact ccgcaacgca gtcatccacg
     2521 gctacaaaac cgagaaagct gattcggcgg tggttgagga tatgcttaca ataatcaggg
     2581 aattacttgg ttctatataa ggtgagcatg ttcagctagg tgattacgtg agaatgatac
     2641 tcaataagat agctaccgct tcatgctctt ttcgacatca ctcaacatct cgatcagatt
     2701 ggcggccaca aacacggcga taccgagcgc gactacgata atgataattg tcagaacgac
     2761 attcattgcc cctccttgcc ggatttatct taaactaaca tatttgtatg gacaggtcaa
     2821 gtttaaacaa tgaggggata gggaattgta aatgtggtta tataaatgaa gtccaggagt
     2881 gccccaagct taagcttggg aattgcagat gatttaggtg agatccgtcc caatctaaag
     2941 attgggtcac cctgacagat tttgatattt ctccggcatt tttcttaata aaacgccagc
     3001 gatgcgggga ggtagcagtc gtcccgggcg aaaggacggt cactcatgag gagttcattc
     3061 tcctccaact tgaatttgtt cttgagaaga aaatccagca cagaaccatt actaccgggt
     3121 acccagacaa tcagcttctc gaatttccgc cctctcatat agttgatggt ctcggccagg
     3181 catgggatga gatactccgg ctttgatgac gttaccggcg ccatgacagt gtctcttgta
     3241 atccaggtat atcccaccgg gttgccgcga tactgataaa tgaaaccgtg aaccagctcg
     3301 ctgtcgatcc agaacttgtg atcctcggga cggcatacac cgcggttggg aagatcgaag
     3361 ctgttaagga tttcaatctg gtcgtaatca tcaatcggta ccgcttttaa atcataatca
     3421 gttttgattt tctgtacccg ttttttctga tcgtatttcc aggtcatcat gggcagcagt
     3481 tgaatcggat acatgccgaa tcgggcatat agctgcacgg caaagggatt gaaggcgaat
     3541 gtcgccaggg aacgtgtgtg aatttcagct tctttgttga agttatagac gcgcttgagc
     3601 agttttctgc cgattccctt gccctgaaag cgttgatcgg tgaagagatg tgccaggtac
     3661 cagtgaccgt cacgaatcat ggcggaagca taacccacca gcctgttgcc ggaaaaagcg
     3721 ccgatagtac cgccggggtc ggttttcagc aagtggcaca tcatggtgtt gcgcttgcgt
     3781 ccgggcttat agggcactgg agacatgcta tgggtctcgc gcagactatt catcgtgcgc
     3841 atgaccagcc gttcggccag ccgtgtatca tcgggcttga tcttccttat gctgagtttc
     3901 atatctccct cctggcatat aagatgtctc tccttagacg attattatgg agagaggttt
     3961 caaatcgcag caaataatcc gattaatagt aaaaaggact tgatgggatg gcttgtcaac
     4021 aagaataaaa tgggcaaaaa gccccaaaat ataggcggtt ttgtacgttt catttataag
     4081 tttttattga ctattgcgag atggccggta aattatactt tcttgatacg tcataatggc
     4141 ggtagcctat gttaaacagg aatgttctta tcttaaatca gaattatgag cccctgactg
     4201 tctgttctgt taagagggcg gttgttatga tgcttttggg caaggttgaa atgatcgaga
     4261 ctatggacgg tcatatgctg cattcagtca gcgctgcgat ccgggtgcct tcggttgtaa
     4321 ggctgggctt ttatgtcaag gtcccccgca agcgcatatt tctttcgcgc aagaatatta
     4381 tcaagcgtga tggacacagg tgtatgtatt gcgggcgctc ggagggctcg atgactgtgg
     4441 accatgtcgt ccccaagcgc atggggggaa gagatacctg ggagaacctg gtgtgtgcct
     4501 gcaccaaatg caacaaccgc aagggcgacc gtacccctga acaggccaat atgaccctga
     4561 tcaaaaggcc caaaaagccc aatcatctca tgtttattca gaggtttatt ggcatttccg
     4621 acgacagctg gaagccctat ctgtttatgg aggattttca ttagatggca caacaggtga
     4681 ttctctccgg aatgcagccg accggttcgc tgcatctcgg aaattatgaa ggggcgttaa
     4741 aaaactggat ctcccttcag gatgattacg aaatgtattg ctgcattgta gactggcatt
     4801 cactgacatc cctgtacgat aaaactgagg tgctgaaaga ccagattttt cagatggcgg
     4861 tcgattatgt tgccgccggc ctggatccgg aaaaatgcgc tatcttcgtg c
//
LOCUS       DSEB01000005            2915 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004377, whole genome shotgun sequence.
ACCESSION   DSEB01000005 DSEB01000000
VERSION     DSEB01000005.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004377"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(1703..2536)
                     /locus_tag="ENO07_00115"
     CDS             complement(1703..2536)
                     /locus_tag="ENO07_00115"
                     /inference="COORDINATES: protein motif:HMM:PF12679.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00397.1"
                     /translation="MMTSDQGLKPVNESRYLRGFSNLFRKENRAWWKTRRWWVNALIW
                     TVALGGLVGMMVFMLPPVAVATGDPNVAAAGGPLAFGLEMGRTVFFELGTMALAIGVI
                     VLSQDLIIEEKQNGLVEWLLAKPVTRRSYLLAKLTASTISILILLVILPATAAYLLLS
                     LGGGSPFPLLPFLQGLGVVSVHSLFFLSLSVMLGTFFNSRPPLLGITLAILLGGNILS
                     GFLQPLLYITPWSLGKVASLTASGASLPIEMLAAPLISTVVWSLLFVLVGLARIEKIE
                     F"
BASE COUNT          787 a          675 c          753 g          700 t
ORIGIN      
        1 aaagaaacct atgccaatca ggatgcaatc cggttgtatg gcaaggcact aacttttttt
       61 ggagaggatg atgggcgacg gtttgatctc ctaaaagcaa gggccgagat attcaattta
      121 atcggcaacc gagaggccca gcgggttgag atcgaggcca tgctcgctct ggccgaaaaa
      181 caagtggata atgtcaaact ggtagatgcg ctccacgcac ttgttcagct gaacctggaa
      241 acggatttac ataaagcgct ggaacctgct caacgcgccc atgaaatttc ccaaaatctg
      301 ggtgatctac caaggcaagc cagatcttct catctgattg gaagagctta cgcagtgaat
      361 ctggatcata accgcgcaat cccgcatctg aaagaagcga ttcagatagc ccgcacggct
      421 ggattgaacc atgaactggt agattatttg aatcacttca gcaggtctca attcaatctt
      481 ggattgcgag atgactcaat gcactcagct caagaagcag ctagcttaag tgatcgggtc
      541 aatgatgagc gcatcaaatt gatcggaacc ataaatctgg gggccgcgta catggcagcg
      601 aagaaccgtg aagcaggatt gccggtgatc caaaacgcag tgggtctggc acgcgagatc
      661 ggtgatcttg aacgggaact ggaagcatta aatattttag gaattatcct tacaggattg
      721 gataggctgg aagaagccgc tgctgtttat ctggaaatga taaataattt tgatcttttc
      781 caattcttcc gtccttttaa tgcggttaat aatattgact atgtttataa ggcaatgggg
      841 gagtatgaaa aaattcaccg acttgttgtg gatctcatgg aaaaagccag ccagggtggg
      901 tactataact gggttgtccg actgacagat tttttcgtga gctcctgttt ccggtttggc
      961 cttaaccagg aagccttaaa aagattggaa tccgccctgc catacgtaga agagctggaa
     1021 aatcaagttt atctggtaac ttacctttat tatttggggt tattttctgc cctggtagga
     1081 gacttcggct gtgcttaccg atatttgcag tctgcccagg tgcagagtga gcgactgcac
     1141 gaatcagtcg tgacaataga tgtctggaac atcagcgcgt atgttgcctt gctggatggg
     1201 aatccctccg ccctgccggt ggggattgaa caggcaaaaa aagggatcgc catgttcgaa
     1261 aatattggtt atgatgtctt ctgggagtgt ttatttatcc tggccgggct gcatctggct
     1321 tctgaccata aagctgaagc ccacgcagcc ttggaaaagg ccctggcaat atgggaggat
     1381 gacctaatct tgatcgacct gatcacacct gaaatgctct actccttcgc atcaaaggta
     1441 ttccgcaata accaccaggc agagaaagct gaaaaatact tgcgcaaggc gtatgatcgc
     1501 atcatgctcg tggcggggaa gatccagagt gatgatctgc gccgaggtta cctggggaca
     1561 gtggatgact tccgggagat tatcaaggaa gccgaagcga ggggatggtg atcggtcagg
     1621 tcaggttgca gaataaaacg gagaggctta taaatttcaa gcctctccgc tccttcaaaa
     1681 attttcagcg aactgttgtt tcttagaatt ctatcttttc aatcctggcc agtccaacca
     1741 gaacaaataa gaggctccac accacagtgg aaatcagcgg ggcagccagc atttcgattg
     1801 gcagcgatgc gccgctggct gtcagggatg caaccttgcc cagcgaccac ggcgtgatat
     1861 ataggagggg ctggagaaag ccggacagga tgttaccgcc taacaaaata gccaacgtga
     1921 ttcccaacag gggagggcgg gagttgaaga atgttcccag catgacagaa agcgacaaga
     1981 aaaacaagga gtgtacggag acaaccccca acccttgaag aaaaggaaga agagggaagg
     2041 gtgatccgcc cccaagcgat aacagcaggt atgcagcggt ggcgggtaaa atgaccagca
     2101 ggataagaat agaaattgtg gaggcagtca attttgccag cagataagag cggcgagtca
     2161 ctggttttgc gagcagccat tctaccaggc cgttttgctt ctcctcgatg atcaggtctt
     2221 gtgagagcac aatcacgccg attgctaggg ccattgtccc caattcaaaa aagactgtgc
     2281 ggcccatttc aaggccaaac gccaatggcc cacctgctgc ggcaacattg gggtctcctg
     2341 tcgccacggc aaccggcgga agcatgaaca ccatcatccc gaccaggccg ccgagggcga
     2401 ctgtccagat cagggcgttg acccaccacc gccgggtctt ccaccaggct cggttttctt
     2461 tgcgaaacag attggaaaat ccccggagat acctggattc atttacaggc tttaatcctt
     2521 gatcagaagt catcatcttg tcctttcacg atatccataa aaacttcttc cagctcgtat
     2581 ttttttcggc tgaattcggt aacaatcaca tttgggtccg acaatatatg gcggagaaga
     2641 ttgttctctg cctcaccttc atcgctgacg ccaatttgcc agtaactaat atcattctga
     2701 tgtgtcacag atgtattcga cacccatggc agtttcgcta atcgctctgt caatccctcc
     2761 ggtgtgccct taaaggatgc ggaatagacc acgccatctt tgccattgag gatttgttcg
     2821 attgggccac tggcgacgat ttgacctttg ttcaggatcg caaccgtgtc gctgacgcgt
     2881 tgaacatcat ccagaatgtg tgtggagtaa aaaat
//
LOCUS       DSEB01000006            2221 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1008032, whole genome shotgun sequence.
ACCESSION   DSEB01000006 DSEB01000000
VERSION     DSEB01000006.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1008032"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..250)
                     /locus_tag="ENO07_00120"
     CDS             complement(<1..250)
                     /locus_tag="ENO07_00120"
                     /inference="COORDINATES: protein motif:HMM:PF00575.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDS00398.1"
                     /translation="MTENNLIEKNTEMSMGQLLKEYSYERPKRGDFIDAEVMQIDDNH
                     ILLDLGAKTDAIITPNELKKTDEKILDTLSEGDIVPVYV"
     gene            complement(247..765)
                     /locus_tag="ENO07_00125"
     CDS             complement(247..765)
                     /locus_tag="ENO07_00125"
                     /inference="COORDINATES: protein motif:HMM:PF02559.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00399.1"
                     /translation="MSTELTYEVGDWIVHNAYGVGQIKKIETKPIHGEKVDTFRVRTK
                     DSVYWLPIEEADSSRIRRVVNKRKLRRALRAVKSQPKEMAKNYKTRNAFIKEVFIDGS
                     FIKMAKLLRDLLALQKKKKWNMTETDAVERIKQRFSREYAVTYEVPIEEGRQKIGELI
                     IEVSNRESKENK"
     gene            complement(903..1274)
                     /locus_tag="ENO07_00130"
     CDS             complement(903..1274)
                     /locus_tag="ENO07_00130"
                     /inference="COORDINATES: protein motif:HMM:PF00072.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDS00400.1"
                     /translation="MVPNHNGNQDRPILILEDDKKLSRVMVRAIENLGFPVLSTSNPA
                     EATKLIEDEGPNLVIVGLENENAGRSIFKKIKQQDEVDLLLLRSDRLDTQTRREVQPT
                     EVVYKPFDTRYLCRRIEDLLS"
     gene            complement(1488..>2221)
                     /locus_tag="ENO07_00135"
     CDS             complement(1488..>2221)
                     /locus_tag="ENO07_00135"
                     /inference="COORDINATES: protein motif:HMM:PF00486.26"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDS00401.1"
                     /translation="SLKAGEPADRSPEELISVLVVDDEPDFLSLYRSILSGNEYDLDV
                     AADGRTAIKMSQVSSPDVILLDWVLPDISGISVCRQIRRWSDVPIIMITSRSAQEDVI
                     AALDAGADDYLVKPFMGDELIARIKAILRRGETLRDKRTADRFSKKGLVIDYNTREVW
                     VSGEKLSLTATEFDLLEFFSRNQRQILKYEQILDQIWQGGEGGTRHALSVHISRLRKK
                     IEPDPDNPKFLVTRWGVGYVFLPDD"
BASE COUNT          532 a          596 c          465 g          628 t
ORIGIN      
        1 acacatacac cgggacaata tccccttcag aaagggtatc taaaatcttc tcatccgttt
       61 tcttgagttc attcggggtg ataatcgcat ccgtttttgc acccaggtcc aacaatatgt
      121 ggttatcatc aatctgcatt acttccgcat cgatgaaatc gccccgctta gggcgttcat
      181 aactgtattc cttcagtaat tgccccatgc tcatctcagt gtttttttca atcaggttat
      241 tctcggtcat ttattctcct ttgactctcg gttggatact tcaattatta gttcgccaat
      301 tttctgacgc ccttcttcga tcggcacctc ataggtcacc gcatattctc gtgaaaatct
      361 ttgcttgatc cgttcaacgg catctgtttc ggtcatattc catttcttct ttttttgtaa
      421 cgccagcaga tcacgcagca gttttgccat ttttataaaa gagccgtcta taaagacctc
      481 tttgataaaa gcattacgcg tcttgtagtt ttttgccatt tcctttggct gagacttcac
      541 agctctcaat gcgcggcgca gcttgcgctt attgacgact cttcgaatcc gagaactgtc
      601 agcctcctct attggcagcc aatagacgct gtctttcgtc cggactctga aagtatctac
      661 cttctcgccg tgaattggct tggtctcaat tttcttaatc tgcccaacgc cataagcatt
      721 gtgcacgatc cagtcgccca cttcataggt tagttcagtt gacatttaca cctccaaatg
      781 gtatctggtt tacatttatc acccaaaacg atcgctaagg gctttacgcc gtaaagcagc
      841 cgaagctgtt ggtcaagctc caactgggat ttagatatta aaaaatcaga tattcccttt
      901 atttatgaaa gcaggtcttc aatccgccgg caaagatagc gtgtatcgaa tggcttataa
      961 accacttctg tcggttgaac ctcccggcga gtttgggtat ccagcctgtc tgaacgcagc
     1021 agcagcagat cgacctcatc ctgctgtttg atctttttaa agatcgacct gccagcattt
     1081 tcattttcca ggccgacgat gaccagattc ggaccttcat cctcgatcag cttcgtcgct
     1141 tcggctggat tgctcgttga taggaccgga aaacccaggt tttcgatcgc ccgaaccatt
     1201 accctggaca attttttatc atcttcaagg atcaagatcg gccggtcctg atttccattg
     1261 tgattgggaa ccattgcccc tgctctcttt cttgctattc agctactgcc atactataaa
     1321 cacaggttat tgagaatttc taaataaact ggcacaaata tctctaaaat gtctcgaagc
     1381 agccctgcag gcaaaaactc attcacagca tgaccaacaa ccgcgaatgc cataaaccga
     1441 atcgggggaa agaaactgtg ctatagaaac tgcgctgagg agaaaaccta atcgtccgga
     1501 aggaagacgt agcccacgcc ccagcgtgtc accagaaatt ttggattgtc cggatctggc
     1561 tcgatcttct tgcgcagtct gctgatgtgt accgaaaggg catgccgggt tcccccctct
     1621 ccaccctgcc aaatctgatc taggatctgc tcgtatttga ggatctgccg ctggtttcgt
     1681 gagaagaact ccagcaaatc gaattcggtg gcggtcaatg agagcttttc accagacacc
     1741 cacacttcgc gcgtgttgta atcgatcacc agcccttttt tcgagaaacg gtctgcggtt
     1801 cttttatccc tcaatgtttc tccccgccgc agaatggctt tgatccgggc gatcagttca
     1861 tcgcccataa atggtttgac caggtagtca tcagcccctg catccagggc agcgatgaca
     1921 tcttcctgcg cgctgcgtga tgtaatcata ataatcggca cgtctgacca acgccggatt
     1981 tgcctgcaaa ctgaaatgcc gctgatatct ggcaagaccc aatccaacaa gatcacatct
     2041 ggcgaggaaa cctggctcat tttgattgct gtgcggccat ctgcggctac atccagatcg
     2101 tattcattgc cagagaggat tgagcggtac aaagacagga aatcaggctc atcatccacc
     2161 accagcactg agatcagctc ctccggagac cggtctgccg gctcgccagc cttaagggat
     2221 g
//
LOCUS       DSEB01000007            2915 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004378, whole genome shotgun sequence.
ACCESSION   DSEB01000007 DSEB01000000
VERSION     DSEB01000007.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004378"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..686)
                     /gene="ftsA"
                     /locus_tag="ENO07_00140"
     CDS             complement(<1..686)
                     /gene="ftsA"
                     /locus_tag="ENO07_00140"
                     /inference="COORDINATES: protein motif:HMM:PF02491.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDS00402.1"
                     /translation="MGRETTFAVVDIGSTKIATLIASRNGQDELSVLGCGTVKVEDIF
                     RKGILSNIDRTARTLAMSVSAAEEMAGIKVQNLTVGFSGEHLRSFNSLGVVPVSKTEN
                     QITENEVEAVIEAAQAVRLPFEREILHVLPQEFFIDQGDGIKDPIGNTGVRLEVQVHV
                     VTASTAATQAIYKAVRMAGYEITNLVLNPIVVGSALLSAEEEEVGTLLIDIGGSATDI
                     AIYYKGAIRHT"
     gene            complement(706..1416)
                     /locus_tag="ENO07_00145"
     CDS             complement(706..1416)
                     /locus_tag="ENO07_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00403.1"
                     /translation="MKKGLYRIGMLAFILGGAVLLAGIYHYDLITVKTAVCKADQGDL
                     PLPVKSYVEGHVGTSILDYPSKAYVDTIFARYPQVSRASVSLNPFGKVVFDYDLKQPI
                     ALISLDLVYGLTARGELVPAQEGDMPILTGLNIGHVRLYEQLDESKIGYALKLAKLIN
                     SNITGIGNAVSSINLGHPSGLSVYVKGCRSEFILGRGDEIMKFKKLAEYLEFFCALGD
                     DIQAVDCRFDNQFILKKD"
     gene            complement(1413..2732)
                     /locus_tag="ENO07_00150"
     CDS             complement(1413..2732)
                     /locus_tag="ENO07_00150"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDS00404.1"
                     /translation="MSGMAEMLHNLGYHITGSDLAASEVTDHLQEIGITVNLQHKAEN
                     VGDANLVVYSSAVPLDNPELEYARQNKIIIVRRAEMLAELMRMKYSIGIAGTHGKTTT
                     TSLIGQIMTDAGMDPTVIVGGRVIQFGTNIKLGRSDYLVAEADEYDRSFLRMTPTDVV
                     LTTLEEDHLDCYEGIDDLKQAFTEFANKVPFFGTVHINMDDPNLVSLIPQINHPVVTY
                     GLASQADYQIDNVTYINNITEFDVRTKKRELGHVKIQLPGFFNAMNSLAAISICMEQE
                     IPFETIKESLGNFRGVNRRFEIIGQVDQYMVVDDYGHHPTEVEATLRAAKRGYNRPVI
                     AIFQPHLYSRTRDFYREFAKALLSADVMIVVKIYPAREKPIEGVTGQLIVDAAKSFGH
                     QNCHYIDDLTMLPEFVLDNIMDKAIIMTIGAGSIYRIAPEILEVLKK"
BASE COUNT          702 a          782 c          688 g          743 t
ORIGIN      
        1 gtgtgacgga tcgctccttt atagtatatg gctatgtcgg tcgcggagcc cccgatatcg
       61 atcagcagcg tcccgacctc ttcttcttcc gccgacaaaa gcgcggagcc gaccacaata
      121 ggatttagaa ccaggtttgt aatctcatac cctgccatcc gaaccgcctt gtaaattgcc
      181 tgtgtagcag cagttgacgc ggttactaca tgcacctgga cctcgagccg aactccggta
      241 tttccgatcg ggtccttgat accatcgccc tggtcgataa agaattcctg cgggaggaca
      301 tggagaatct cccgctcaaa gggcaagcgc actgcctgag cggcctcgat gaccgcctcc
      361 acctcgtttt ccgtgatctg gttttcagtt ttggacaccg gcaccactcc cagggagttg
      421 aaagaccgca aatgttcccc cgagaagccg accgtaagat tttgaacctt gatcccggcc
      481 atttcctccg ccgcagatac cgacatggcc agtgtcctgg ccgtccggtc aatattcgag
      541 aggataccct tgcggaaaat gtcttcaact ttcacggttc cgcacccaag cacagacagt
      601 tcatcctggc cgttcctgct ggcgatcaag gtagcgatct tggtggaacc gatgtcaact
      661 acagcaaatg ttgtttctct tcccatgaca tagccctcta tttcgttaat cttttttcaa
      721 aataaattga ttatcaaatc tgcaatccac cgcctggata tcatctccca gcgcacaaaa
      781 gaactcaagg tattcggcca gcttcttgaa cttcatgatc tcatccccgc gtcccagaat
      841 aaactcgctg cggcagcctt tcacataaac tgacagaccg gacggatgtc ccagatttat
      901 tgatgaaacg gcatttccaa tgcctgttat attcgaattt atgagtttgg ccagtttcaa
      961 tgcatagcca atcttgcttt catcaagctg ttcatataat ctaacatgcc caatattcag
     1021 gccggtaaga atcggcatat ccccctcttg agcaggtaca agttcgcccc ttgcagtgag
     1081 accgtagacg aggtcgagac ttataagagc gatcggctgt ttcaagtcgt agtcaaatac
     1141 caccttaccg aatggattca aactcacgga agcccgggaa acctgcggat accgggcaaa
     1201 gattgtatcc acataggctt tcgaaggata atcgaggatc gaagtgccca catgcccctc
     1261 gacatagctt ttaacaggaa gcggtaaatc tccctggtcg gctttgcaaa ctgcggtttt
     1321 gaccgtaatc aagtcgtaat gataaatgcc cgcgagaaga acagctcctc ccaggataaa
     1381 agccagcatg ccgattctgt ataaaccctt cttcactttt tcagcacctc gagtatttcc
     1441 ggagcaatgc gatagatcga gcccgctccg atggtcatta ttattgcctt gtccattatg
     1501 ttatccagta cgaattcggg cagcatggtg agatcatcaa tataatgaca gttctgatgg
     1561 ccgaaactct tggccgcatc gacaatgagc tgtccggtta caccctcgat aggtttctcg
     1621 cgagccggat agattttcac cacgatcatc acatccgcag acaggagcgc tttggcgaac
     1681 tcgcgataga aatcacgtgt gcggctgtat agatgcggct ggaagatggc tatcaccgga
     1741 cgattatagc cgcgtttggc agcgcgcagg gtggcttcaa cctcggtggg atgatggccg
     1801 taatcgtcaa ctaccatata ctgatcgacc tggccgataa tttcaaagcg tcggttgacc
     1861 ccgcggaagt tgcccaggga ttccttaatg gtctcgaatg gtatctcctg ttccatgcag
     1921 atcgagatag ccgccaggct gttcatggcg ttgaagaagc ccggcagctg aattttgaca
     1981 tgacccagct cacgcttctt ggttctgacg tcaaactcgg tgatattatt aatataagtt
     2041 acattatcaa tctgataatc cgcctgtgaa gccaggccat aggttaccac gggatgatta
     2101 atctgcggga tcagcgagac cagattggga tcatccatat tgatatggac agtcccgaag
     2161 aaaggcactt tattggcaaa ttctgtaaag gcctgtttga ggtcgtcgat cccctcataa
     2221 cagtcgagat gatcctcctc caaggtggta aggaccacat cggtgggggt catacgcagg
     2281 aatgaacggt cgtactcgtc ggcctcggcc accaggtaat cggaccgtcc cagcttgata
     2341 ttggtgccga actgtataac gcgtccaccc actataacgg tcggatccat accggcatcg
     2401 gtcataatct gaccgatcag cgaggtcgtg gtagtctttc catgcgtgcc ggcgattccg
     2461 atggaatatt tcatgcgcat gagttcggcc agcatctcgg cccggcgcac aattattatc
     2521 ttattctggc gggcgtattc gagctcggga ttatccaggg gcacagcaga tgagtaaacc
     2581 acgagattgg catcgccgac attttccgct ttgtgctgca ggtttactgt tataccgatt
     2641 tcctgcaggt ggtcggtgac ctcgcttgct gccaggtcac ttccggtgat atgatagccg
     2701 agattgtgca gcatctcggc catacccgac attccgatcc cgcctatgcc cacaaagtgc
     2761 agttttctta attttccgaa ttttaccact ttaaaacctc aataattttc tctgcgattt
     2821 ttttggatgc atccggatca gccagcgacc tggccgcctc cgacattttc ttcaatcgat
     2881 ccgggtctga aaaaagcccg gacacatatt catga
//
LOCUS       DSEB01000008            1686 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1014718, whole genome shotgun sequence.
ACCESSION   DSEB01000008 DSEB01000000
VERSION     DSEB01000008.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1014718"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            60..>1686
                     /locus_tag="ENO07_00155"
     CDS             60..>1686
                     /locus_tag="ENO07_00155"
                     /inference="COORDINATES: protein
                     motif:HMM:PF07228.10,HMM:PF16697.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HDS00405.1"
                     /translation="MLKLVGTDGNKYYSFDLKPGNYKVGRSKDADICIMHKTVSGNHA
                     RLSVEDSGRILINDVGSRNGTVVNGRKISEPTKLSDGDSIMFGLTEFRVRDSELPDKG
                     EVSSQRISFTGDVPENSIFVPIDEAMRPLPRKLSEMPELMPTIFDMAKLLGSDEPRGI
                     LLDKALKLICRLIPADRVIVIMVSEDQQDIDIAACHTSSVKGDDEITLSRTIIVDIIE
                     NKNSLLIKNPLTDPKFSTQNSIISADIKSVMAVPLFDEGRVLGILYADTSNPAHEYND
                     DYLRIMATFGNLLGSRLLNYELSIEREAKQVLSAELRQASKIQRNLLPSEPLEIDGYK
                     LYAYQEQSRQVGGDLYDYKILPDGSLVFVLGDVTGKGMGAALLMSNILAAFRVLYEKE
                     SFTPATAVKTISTQLLSYSKSNSFVTLFVGLLDPDSGIMRYVNAGHNPPLLLKQDGAL
                     EHMEPTGMMAGAFEFAEWEEKTVELAKGDSIFIFSDGLSEADRSGSGDQFGDERTEKA
                     ALESKDKDPRGLIEDMAEQLKQFMGDAPQSDDIT"
BASE COUNT          438 a          371 c          462 g          415 t
ORIGIN      
        1 ggtcatcatt cttcaatatt gaccatcttg gccatgagcc aaattgcaga cgggtgatta
       61 tgctcaaatt agtggggacc gacggaaata aatattatag cttcgacctg aagccgggaa
      121 actataaagt cggccggagc aaggatgccg atatatgcat catgcacaag acagtgtcgg
      181 gaaatcatgc ccggctttca gtcgaggatt cgggcagaat actgataaat gatgtcggca
      241 gccgcaatgg tacagtagta aatggaagga agatctctga accgaccaaa ctttcggatg
      301 gtgatagtat tatgttcggg ttgacggaat tcagggtcag ggatagtgag ctgccggaca
      361 agggcgaagt gagcagccag aggatttctt ttaccggaga tgtgcccgag aattcgatct
      421 ttgtgccgat cgacgaagcc atgaggccct taccccggaa gctgtccgaa atgcccgagc
      481 tgatgcctac tattttcgac atggccaagc ttctggggtc ggatgaaccc aggggtatcc
      541 ttctggataa agctcttaag ctgatctgca ggctcattcc ggcggaccgg gttatcgtga
      601 tcatggtctc ggaagaccag caggatatcg atatcgccgc ctgccacacg tcatcagtaa
      661 aaggggatga cgaaatcaca ttgtccagaa cgatcatcgt cgatatcatc gagaataaaa
      721 actcgctttt gatcaaaaat cctctgactg atcccaagtt ttcgacccag aattcaatta
      781 tttctgcgga tataaaatct gtgatggctg tgccgctctt tgatgaggga cgcgttctgg
      841 ggatacttta cgctgataca agcaatccgg cgcatgagta caatgacgat tatctgagaa
      901 taatggctac ctttggaaat ctgctgggct cacgtctttt aaactatgag ctctctattg
      961 aacgcgaggc caagcaggtt ttaagtgccg aattgcgcca ggcttcaaag attcagcgca
     1021 atctgctccc ctcagaaccg ctggaaatag acggctacaa actgtatgcc taccaggaac
     1081 agtcgcgcca ggtgggcggg gatctttatg attataaaat tcttcctgac gggtcattgg
     1141 ttttcgttct gggggatgtt accggaaaag gtatgggtgc tgcgctttta atgtcgaata
     1201 ttctggccgc cttccgtgta ttgtatgaaa aggagagttt tacgcctgca accgcggtga
     1261 agacgatttc gactcagcta ttgtcgtata gcaaatccaa cagctttgtc accctgtttg
     1321 tggggttgct tgatccggat agcgggatta tgcgctatgt caacgcgggc cataatccac
     1381 ctctattatt gaaacaggat ggcgccctgg agcatatgga accgaccggg atgatggcgg
     1441 gcgccttcga atttgccgag tgggaagaga agacagttga actggcaaaa ggtgactcga
     1501 tatttatatt ttctgacggt ttgagcgaag ctgaccggag cgggtcaggc gaccagttcg
     1561 gggatgagcg cacagagaag gccgccctgg aatcgaaaga caaggatccc cgcggtctta
     1621 ttgaggatat ggcggagcaa ttgaaacagt tcatgggtga tgctccgcaa tcggacgata
     1681 ttacca
//
LOCUS       DSEB01000009            2218 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1008049, whole genome shotgun sequence.
ACCESSION   DSEB01000009 DSEB01000000
VERSION     DSEB01000009.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1008049"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..233)
                     /locus_tag="ENO07_00160"
     CDS             complement(<1..233)
                     /locus_tag="ENO07_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDS00406.1"
                     /translation="MLEKIRDLAENYYRRMVELRRYFHARPETAFEEIETSKKIKAEL
                     SLLKIKSSSHAKTGVLGVLQGTKEKPVCALRSD"
     gene            complement(247..972)
                     /locus_tag="ENO07_00165"
     CDS             complement(247..972)
                     /locus_tag="ENO07_00165"
                     /inference="COORDINATES: protein motif:HMM:PF04493.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HDS00407.1"
                     /translation="MRFARMHQWTTDLKEAARIQEELVDLVEVRPPDREFETIAGADV
                     AYSKGDEAIFASVVVMKFPELVPLEKVRAQSYVSFPYIPGFLFFREGPVLAKALERLE
                     SVPDVIMFDANGIAHPKRIGMASHFGVMLDIPVIGCAKKKLIGNFRTLPDELGARAPL
                     EAENEEIVGYAVRTRAGVKPLFVSAGHKIDNETAADVVSATTRGYRLPEPLRIAHILV
                     NKMRRNYDSRQKPHRGQQGREYR"
     gene            complement(1177..1767)
                     /locus_tag="ENO07_00170"
     CDS             complement(1177..1767)
                     /locus_tag="ENO07_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDS00408.1"
                     /translation="MSEIKWMTDNSACPAEIEFRRIERSELQDEDTAYVQNSVFRSLK
                     KVINSSYTRYELFMKLCSKLDKFFAINRASLALYDEENQVMSITHIQINHEFRSGVHL
                     NIVAGKTLMKKVLDSGHAYVRDYAQDIEAIELEKKILMDKDSRSLAIIPLVHGDKKIG
                     TLNITSSSYQAFSILESHIFDYFFHKTSEKMSELPQ"
     gene            complement(1760..2128)
                     /locus_tag="ENO07_00175"
     CDS             complement(1760..2128)
                     /locus_tag="ENO07_00175"
                     /inference="COORDINATES: protein motif:HMM:PF00072.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDS00409.1"
                     /translation="MPDIKKKMRILMVDDNLSFLKSTRLALSKLGFECRISQTVEQAL
                     ELLHNEKFDLIICDYFMPESNGREALKKLGDSCVECKLILTSKYPLDIKFKKSDRFIF
                     VDKLGLPGWLSEKYSEVLYV"
BASE COUNT          547 a          562 c          491 g          618 t
ORIGIN      
        1 atatcagatc tcagagcgca tacaggtttc tcctttgttc cctggagaac gcccagaacg
       61 ccggttttag catgcgagct tgattttatt ttgagcaggc tcaattcagc tttgattttt
      121 ttggaagttt caatttcttc gaatgcggtt tccgggcggg catgaaaata gcgccgcagt
      181 tcgaccatac ggcggtaata attctcggcc aaatctctta tcttttcgag catattttga
      241 gccttctcaa cggtattccc tgccctgctg gcccctgtgc ggcttctgcc tgctgtcata
      301 attgcggcgc attttgttaa ccaggatatg agcgatcctc aacggctcag gcaacctgta
      361 gccccgggtc gtggcagaca caacatctgc agccgtttca ttatctattt tatggccggc
      421 ggaaacgaaa agcggtttca cgcccgccct tgtacgcaca gcatagccga caatctcctc
      481 gttttccgcc tccagaggcg cccgcgcccc cagttcatcc ggcaaagtcc tgaaatttcc
      541 gatgagtttc ttcttcgcac agccaatgac cgggatatcg agcatgaccc cgaaatgaga
      601 agccatgccg atccttttag ggtgagcaat gccgttggca tcaaacataa tgacgtcggg
      661 cacagattcc agccgctcca gagccttggc cagaaccggc ccctcccgga agaatagaaa
      721 tcctggaata tacggaaaac tgacatagga ctgcgcccgc accttttcca ggggcaccag
      781 ttcgggaaac ttcataacca caacactggc gaatatcgct tcgtctccct tggaataggc
      841 aacatcagca ccggcaatcg tctcaaattc cctgtccgga ggcctcacct ctaccaggtc
      901 aaccagttcc tcctgtatcc tggcagcttc tttgagatca gtggtccact gatgcattcg
      961 agcgaatctc ataacatccc ctttgaggcc gaaaatggcg accgcttatt ttaacattca
     1021 aattagataa cacctgaatc ttatggatgt tcaaagcttc cttttaatcc tgatacggcc
     1081 atattaaaca gattgaatgt ataattcaac aaaagatttt ggagtgtgtc gaatccgtca
     1141 aatattcaca tactcgtatg gatgctcgcc tgcatgttat tggggaagtt cagacatttt
     1201 ttctgaggtc ttgtgaaaga aatagtcgaa gatatgcgac tccagaatgc tgaaggcctg
     1261 atatgagctg gatgtgatgt tcagggtgcc gattttcttg tcgccatgga cgagcggtat
     1321 tatggccagg ctcctggaat ctttatccat caatattttc ttctccagtt caatcgcttc
     1381 gatatcctga gcataatccc ggacatacgc atggccgcta tccaaaacct ttttcatcag
     1441 ggtcttaccc gcaactatat tgaggtggac gccgctcctg aattcgtggt ttatctgaat
     1501 gtgggtgatg ctcatcacct ggttttcctc gtcatacagg gccagggagg ctctattgat
     1561 ggcaaagaac ttatcaagct tcgagcagag cttcataaag agctcatagc gggtataaga
     1621 gctgttaatg accttcttca ggctgcggaa aaccgaattc tggacgtaag cggtgtcctc
     1681 atcctgaagt tccgaccttt caatcctgcg gaactctatt tccgcagggc aggccgaatt
     1741 atcagtcatc catttaattt cagacataca gtacttccga atatttctcc gacagccatc
     1801 ccgggagacc gagcttgtcg acaaatatga aacggtcgga ttttttaaat tttatatcca
     1861 gcggatattt agaagtcaga atcagtttgc attcgacgca ggaatcgccg agctttttta
     1921 acgcctcccg gccattgctc tcgggcatga aatagtcgca aataatcaaa tcgaattttt
     1981 cattgtgaag cagttccagg gcttgttcga cggtttgaga aattcggcac tcaaagccga
     2041 gtttgcttaa ggccaggcgg gttgatttca ggaaactgag gttgtcgtca accatcagaa
     2101 ttctcatttt cttcttgata tccggcataa tacttccaaa aataatgatt tctctaatcc
     2161 aaaactgccg tttccggctt caggttatat tttttcacct tgcgccaaag tgtagtcc
//
LOCUS       DSEB01000010            2523 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1006044, whole genome shotgun sequence.
ACCESSION   DSEB01000010 DSEB01000000
VERSION     DSEB01000010.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1006044"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..240
                     /locus_tag="ENO07_00180"
     CDS             <1..240
                     /locus_tag="ENO07_00180"
                     /inference="COORDINATES: protein motif:HMM:PF01910.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HDS00410.1"
                     /translation="VIDIIDKSGLPYQTHALGTLVEGEWDELMALVKKCHMKLREDFS
                     RVYTRINIDDREGYKDRITGKIAELEEALGRQIKK"
     gene            280..582
                     /locus_tag="ENO07_00185"
     CDS             280..582
                     /locus_tag="ENO07_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015879858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HDS00411.1"
                     /translation="MVFSSAGSQIEANHIAETLINKKLAACVSILPGVTSHYCWKGKK
                     ESSYEVSIIIKSVKDKFEELKKEIIELHSFEVPEILMLPVVDGNEDYLSWLKEVMA"
     gene            607..1041
                     /locus_tag="ENO07_00190"
     CDS             607..1041
                     /locus_tag="ENO07_00190"
                     /inference="COORDINATES: protein motif:HMM:PF03479.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDS00412.1"
                     /translation="MLYKKVAGGYLLRLERGEEVMESLLKFIKKFKIRGGFMVGLGAC
                     EDLKLGYFDAVKGEYRDKEFPGEFEVTNLTGNIAYLDKEPIVHAHITLSDDKFKAIAG
                     HLWHGIVSGTMEIMIMTFGIKIRRAKDDETGLNLLKLGKVKV"
     gene            1038..1838
                     /locus_tag="ENO07_00195"
     CDS             1038..1838
                     /locus_tag="ENO07_00195"
                     /inference="COORDINATES: protein motif:HMM:PF01887.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00413.1"
                     /translation="MNGKLSTIALLTDFGSQDSYVAEMKGVILSINSNVNIIDISHNI
                     EPQNILQASLMLKRSYGFFPSRTIFICVVDPDVGSGRKIILIEAEEYSFVAPDNGLLW
                     LTANELSVNKIISLENYDYFLNPTPSTFEGRDKMAPAGSYLSLGIEPGQFGPIQSGIE
                     KLEIPKTQIEKNLLIGEIIYFDRFGNAITNISMDDLMENEPRNLPLVIFGPDEIGHIV
                     STFSDAANGSAVAYFGSSGYLEMAIFNGNFRLKYSARPGDRVVVDYAK"
     gene            1828..2331
                     /locus_tag="ENO07_00200"
     CDS             1828..2331
                     /locus_tag="ENO07_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDS00414.1"
                     /translation="MPSSDGRPTWDQYFMSIAKLASTRSTCLRRKVGAVIVKNKRILA
                     TGYNGAPRHMQHCLDIGCLREKLNIKSGERHELCRAIHAEQNAVIQSAVSGVSIEGAD
                     IYCTTFPCVLCSKIIINAGIRNIKVLEGYPDELSQQMLDEAGIKVEYLDSAELISIDK
                     EEVEELL"
     gene            2328..>2523
                     /gene="nrdR"
                     /locus_tag="ENO07_00205"
     CDS             2328..>2523
                     /gene="nrdR"
                     /locus_tag="ENO07_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDS00415.1"
                     /translation="MRCPFCGHQEDKVVDSRTAQDGRAVRRRRECLGCGRRFTTYEYV
                     ENLSLSVVKRNQARELYDRTK"
BASE COUNT          691 a          549 c          675 g          608 t
ORIGIN      
        1 gtcatagata taatagacaa atcgggcctg ccataccaga cccacgctct cggcacgctg
       61 gtcgaagggg aatgggatga gctgatggcc ctggtcaaaa aatgtcatat gaaactgcgc
      121 gaggatttca gccgtgttta cacgcgcata aacatagatg accgcgaagg ttataaggac
      181 cggattaccg gtaaaatcgc ggaattagaa gaggcattag gacggcaaat caagaaataa
      241 agcaggcgag gttttgcaat ggacaattct gattttgtaa tggtcttttc atctgccggt
      301 tcgcaaattg aagccaatca tatcgccgag acacttatca ataagaagct ggcggcatgt
      361 gtgtcgatac ttccgggagt cacatcccat tattgctgga aggggaaaaa ggaatcctca
      421 tatgaagtgt caattattat caaatccgtc aaggacaagt tcgaggaatt gaaaaaggag
      481 ataattgagc ttcacagttt cgaggttcct gaaattctga tgctgccggt tgtggacggc
      541 aacgaggatt atttgagctg gttaaaagag gttatggctt agcatggatt cccaaaagga
      601 ggattgatgt tatacaaaaa agtcgctggc ggttatcttc tgaggctcga gcgcggtgaa
      661 gaagtaatgg aatcgctttt aaagttcatc aagaagttca aaatcagggg cggattcatg
      721 gtcgggctgg gggcctgcga ggatttgaag ctgggctatt ttgatgcggt caagggcgag
      781 tatagggata aagaatttcc gggcgaattc gaagtcacca acctcaccgg caatatagct
      841 taccttgaca aggaaccgat cgtacatgcc catataaccc tttccgatga taagttcaaa
      901 gcgattgccg ggcatctctg gcacggtatt gtttcgggga cgatggaaat tatgattatg
      961 actttcggga taaagatccg gcgtgcaaag gatgatgaaa ccggtttaaa tctactgaaa
     1021 ttgggcaagg taaaagtatg aatggtaaat tgtcgaccat agcgctgcta accgatttcg
     1081 gcagccagga cagctatgtg gccgagatga aaggtgtgat tctttccata aattccaatg
     1141 tgaatattat tgatatcagc cataatatcg agccccagaa tattctgcag gcttcactga
     1201 tgcttaaacg ttcctatggc tttttccctt cccgcacgat cttcatttgt gtggttgacc
     1261 cggatgtcgg ctcgggcaga aaaataatct tgatcgaagc cgaggagtat agttttgtag
     1321 ctcccgataa cggtctgctg tggctgaccg ccaatgagtt gagcgttaac aagattatat
     1381 cgctggagaa ttacgattat ttcctgaatc ccacaccttc gacatttgag ggtcgggaca
     1441 aaatggctcc ggcgggatca tatctgtccc ttggaatcga gcccggacaa tttggtccga
     1501 ttcaatctgg aatagaaaaa ctggaaatac cgaaaacaca gattgaaaag aatctgctga
     1561 ttggcgaaat catatatttc gacaggttcg gcaacgccat cacaaatatc tccatggacg
     1621 accttatgga gaacgagccg aggaacctcc cgctggtgat atttggcccg gatgagatcg
     1681 ggcacattgt atcgactttt tccgatgctg ctaatggctc ggcggtggcg tatttcggca
     1741 gttccggtta tctggagatg gccatcttta acggcaattt caggctcaag tactcggccc
     1801 ggccggggga cagggtggtg gttgattatg ccaagtagtg acggccgtcc cacctgggat
     1861 cagtatttca tgtcgatcgc aaagctggcc tccacacgct ccacttgcct gcgcagaaag
     1921 gtgggcgcag taatagtgaa gaataaacgc attctcgcca ccggctataa cggcgccccc
     1981 cgtcatatgc agcactgtct cgacatcgga tgcctgcggg aaaagctgaa tataaaatcc
     2041 ggtgaacgtc acgaactctg ccgcgccatc catgccgagc agaatgcggt aatacagtcg
     2101 gcggtttcgg gagtgtcgat cgagggtgca gatatttact gcaccacatt cccctgcgtg
     2161 ctatgttcca agattatcat caatgcgggt atcaggaaca tcaaagtact ggagggttac
     2221 ccggacgagc tttcacagca gatgctggat gaggccggaa tcaaggtcga atatctcgac
     2281 agtgcagagc tgatttcaat agacaaagaa gaggtggaag aacttctatg agatgcccat
     2341 tctgcggaca tcaggaagac aaagtggttg actcacgtac tgcacaggat ggacgcgccg
     2401 tgaggagacg ccgtgaatgt ctgggctgcg gacgcaggtt cacgacctat gaatatgtcg
     2461 aaaacctttc gctctcggtg gttaaacgca accaggcccg cgagctgtat gaccgcacca
     2521 aac
//
LOCUS       DSEB01000011            2219 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1008044, whole genome shotgun sequence.
ACCESSION   DSEB01000011 DSEB01000000
VERSION     DSEB01000011.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1008044"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            978..>2219
                     /locus_tag="ENO07_00210"
     CDS             978..>2219
                     /locus_tag="ENO07_00210"
                     /inference="COORDINATES: protein motif:HMM:PF04055.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDS00416.1"
                     /translation="MAKIPILKESTEMMRHHWDGRPYPSLVYVETTNFCNARCKFCLY
                     ERMERPIKFLRPADFVEIAHKVKERGLKIGAMFCFGEPLADRDLFNKIEYGRQIGVMT
                     NYLGLNTNASLLRPEVFDKIFQACSNITLSFPNVGSEFERLTGLKWERCYKNAIEFIR
                     YRDKNKPGFKVEIGCNEVTGADEASVRKAFAGYRVEWAKDAEIKWGGKIITGVIDRSI
                     MYHSWTCDGYKGAMQIKPNGDCCFCAYDVIRGETNFANILKDDWETIEANFKKLWRRP
                     SSLCLRCDFWWNYYQVKAAGWRRGDHIDSSWQAAYRDGIKSFWENNHQKHEIRFLTGS
                     SISNVANFLGFADFLRPGMRILNIGLGTGRCSKELNARDIIVDGLDISRNAIENCLDY
                     IDRGFTDPRQLPSRQYDLAICN"
BASE COUNT          596 a          508 c          586 g          529 t
ORIGIN      
        1 ggtataatgg gcagacattt ccccgccttt ttcaagggta agaggttcct ggatatcggt
       61 tgctcaaaag gatacttttg tttcgcccac agaaaggact tcgaacagat aaccgggata
      121 gattcagact ggctggcgat cgatttatgc agcaaggtgg cccggtgcta ccccggcaat
      181 tatgacttca aaaatgtgag tttccgagat tttcatgact ctatcagctt cgacagaata
      241 tttataggca acggaccgca tcatctatac agggaaatcg ggagcaatgc ctggattaat
      301 aaattggccg ccctctcaag cgacctggtg ctgaccgagg gccccataaa catccagtgc
      361 cccgacctcc aggctcagcc ggacaaatat ctccggaatt atgattcatt catactccgc
      421 atgaaatctc attttgacct tttgggcata cattccacca ccagatatac tccggagaga
      481 tatcttattc tctggaagcg gagggaagct ggccgcctgg gtctccgaga aaacggattg
      541 atatttaaaa gatttaagca cgatgactat gtcgataaca accaggtcga catctctata
      601 gctgccagct cgcccgtgag caacggcctg gtcgcctttg gagaggacgg ctggtatgaa
      661 aaaagatgtt cagcaaaacc attcaaatat aaagaaaatc aggagcggct gttcaaaagg
      721 cactgcctcc atcaaatata tttgtccctg ctgggatata tagatatcga tcccgggacc
      781 atcaatttct ttcaaggctc agaattactc ttcgataaat ccggagttat gccaatagct
      841 gaaatcggga gcagacatat tggagcttat ctgaagctgc tgcattattc ctatgacatt
      901 ttgagcccga aatttgagga gataatcaat tcggctctca catccaagga cccgaacaga
      961 ataaaggaag cgtatgaatg gctaagatcc cgattctaaa agaaagcacg gagatgatga
     1021 ggcaccattg ggacggccgt ccttatccca gtcttgtcta tgtggagacg accaactttt
     1081 gcaatgcccg ttgcaagttc tgtctgtatg agcgcatgga gcgcccgata aaattcttgc
     1141 ggccggcaga ctttgttgaa attgcccata aggtcaagga gcgcggcctg aagatcgggg
     1201 ccatgttctg cttcggtgag cccctggccg atcgggatct gtttaacaag atcgagtatg
     1261 ggcgtcagat tggagtcatg acgaattatc tcggacttaa cacgaatgcg agcctgctgc
     1321 ggccggaggt cttcgataaa atatttcagg catgctcgaa tattacactc tccttcccca
     1381 atgtgggcag cgagtttgaa cggttgaccg gattgaagtg ggagcgctgc tataaaaatg
     1441 ccatcgaatt catacgatat cgtgataaaa ataagcctgg atttaaggtt gagatcggtt
     1501 gcaatgaggt gaccggcgct gatgaagcct cggtcaggaa agcttttgcc ggctatcgcg
     1561 tggaatgggc caaggatgcc gagatcaaat ggggcggcaa aatcatcacc ggagtaattg
     1621 accgctcgat tatgtatcac tcctggactt gcgacggcta caagggcgcc atgcagataa
     1681 aaccgaacgg ggattgctgc ttctgcgcct acgacgtgat ccggggcgag accaatttcg
     1741 ccaatatcct caaagatgac tgggagacga tcgaggccaa tttcaaaaag ctctggagaa
     1801 ggccctcgag cctttgtctg agatgcgatt tctggtggaa ttattatcaa gtgaaggccg
     1861 caggatggcg gcggggtgat catattgaca gcagctggca ggcggcctat agggatggca
     1921 taaagtcatt ctgggagaat aaccaccaga agcatgagat caggtttttg accggcagca
     1981 gcattagcaa tgtggcgaat ttcctgggct ttgcagattt cctgaggccg ggaatgcgga
     2041 ttctcaatat aggcctgggt accgggcggt gctcgaagga gctgaatgct cgggacataa
     2101 tagtcgacgg cctggatata tcccggaatg ccattgagaa ttgtctggat tacatagata
     2161 gaggcttcac ggacccccgc cagctaccga gcaggcagta tgacctggcc atatgtaac
//
LOCUS       DSEB01000012            2139 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1008730, whole genome shotgun sequence.
ACCESSION   DSEB01000012 DSEB01000000
VERSION     DSEB01000012.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1008730"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..996)
                     /locus_tag="ENO07_00215"
     CDS             complement(<1..996)
                     /locus_tag="ENO07_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006795903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDS00417.1"
                     /translation="MDLEILSRIQFGFTIGFHYLFPVLSIGLGLFLVIMEGAYLKTKN
                     QMYHSMTRFWVKIFALIFALGVATGIVMEFQFGTNWGTYSRFVGDVFGSALAAEGIFA
                     FFLESGFLAILVFGWDKVKPTTHFISTILVAFGAHFSAIWIVVANSWQQTPAGHHIVG
                     TGENARAEIVDFWQVVFNPSFLDRISHVYMGAWQAAAFFVLSVSAFYLLKNKHKDFAR
                     GSMKIGIVIALIASILQLVTGHSSANMVAEHQPAKLAAMEAHYPESAPAGLYLFGWVD
                     EGEEKVTGIEIPGLLSFLISWDPAEPVTGLNAFEPEDRPPVNLVFQSYHIMVAIGM"
     gene            complement(1207..2091)
                     /locus_tag="ENO07_00220"
     CDS             complement(1207..2091)
                     /locus_tag="ENO07_00220"
                     /inference="COORDINATES: protein motif:HMM:PF08327.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00418.1"
                     /translation="MFAKGTIGLKVVINAPDSVVFKHLTDPGLLCRWFCDEAEIEQRV
                     GGGFVFGGPHSFMSTFIEKAGIAVIEEYNPPALFKFSFSLNGSPTSMEFQLTAEQDRT
                     SLLLTHRDIPRESLMMDALIVSLSNLVHLVETGMAGHRLNYLTDITDEKIEREIKIES
                     SPEAVFKALIDKNSLSLWFTDTIREVDPQIGGVYDVGWRSSDGKQIGPIKITQLEDNR
                     RLGYSWFYDPDGGSQAVWELTPDENGTRVRLTHEGFKRGRYNKDYYQGWHAYMLALKG
                     FVEKGCRPFEVVEGNWEF"
BASE COUNT          573 a          576 c          496 g          494 t
ORIGIN      
        1 catgccaatc gccaccatga tatgataact ctgaaagaca agattgaccg ggggccgatc
       61 ttcgggctca aacgcattca atccggttac cggctctgcc gggtcccagg aaattaagaa
      121 actcaacagg cccgggattt caatgcccgt tactttttcc tcgccttcgt ccacccaccc
      181 gaacagatac agcccggcag gagcgctttc cgggtaatgg gcctccatag cggccagctt
      241 ggcaggctga tgttcggcca ccatattggc gctggaatgg ccggtcacaa gttgaagaat
      301 agacgctatg agtgcgatca cgatgccgat tttcatcgac cctctggcaa aatccttgtg
      361 cttgtttttc aacagataga aagcgctgac gctcagcaca aaaaaggctg cggcctgcca
      421 tgcacccatg tagacatggc tgatgcgatc cagaaaggat ggattgaaaa ccacctgcca
      481 gaaatccaca atctcggccc tagcattttc gcccgttccc actatatgat gcccggcggg
      541 cgtctgctgc cacgagttgg caaccacgat ccagatggct gagaaatgtg ctccgaaagc
      601 aaccaggata gtcgagatga aatgggtggt cggcttgacc ttgtcccatc cgaaaacgag
      661 tatggccaga aaaccggatt cgagaaaaaa cgcgaagatg ccctcggccg cgagcgcgct
      721 gccgaaaaca tcgccgacaa agcgggaata ggtgccccag tttgtgccaa attgaaattc
      781 catcacaatc ccggtagcca ccccgagagc gaagattaaa gcgaaaatct ttacccaaaa
      841 gcgggtcatg ctgtggtaca tctggttctt tgtcttgagg taggcacctt ccatgataac
      901 cagaaaaagg ccgagcccta tactcaaaac agggaaaaga taatggaagc caatcgtgaa
      961 gccgaattga atgcgcgata aaatttccag atccatcagc cgggcactcc cccagattgc
     1021 agcgctgatc tcatttccaa gcgcctgtta gtcccacacg taaattttaa taatgtttag
     1081 aaatttggtc aagccaaatt attctcactg cagtccaccg cagttcgatt attccatatt
     1141 agttgaatac gcagtgtgga aaccctctgg cgggaatatt taaaccagat tatatggttg
     1201 gcaattctaa aattcccagt tgccttcaac cacctcaaat ggccggcatc ctttctcgac
     1261 aaagccctta agagccagca tatatgcgtg ccaaccctga tagtagtcct tgttataacg
     1321 gccgcgcttg aaaccctcat gagtgagccg cacacgtgtg ccattctcat caggcgtcaa
     1381 ctcccataca gcctgactgc ctccatcggg atcataaaac cagctgtatc caagccgccg
     1441 attatcctcc agctgggtga tttttatcgg acctatctgc ttgccgtcac tgctgcgcca
     1501 gccgacatca tagacacccc cgatttgcgg atctacttcc cggatcgtat cggtaaacca
     1561 cagggagaga gaattcttgt cgatcagggc cttgaaaact gcctccgggg aactctcaat
     1621 cttaatttcg cgctcgatct tctcatcagt tatatcggtc aggtaattca agcggtggcc
     1681 ggccattccg gtctcgacca gatgtacgag gttggaaagc gagactatca gggcatccat
     1741 catcaaggat tcacgcggta tatctcgatg agtcaaaagc agggaggtcc tatcctgctc
     1801 tgcagtaagc tgaaattcca tactggtcgg actgccattc agactgaaag agaatttgaa
     1861 aagggcgggc ggattatatt cttctattac ggcaattcct gctttctcga tgaaagtgga
     1921 catgaaggaa tgcgggccgc caaagacaaa tccgcctccc acccgctgtt cgatttcagc
     1981 ctcgtcgcaa aaccaccggc acaaaagacc ggggtctgtc agatgcttga atacaacact
     2041 atcaggagcg ttgattacca ctttgaggcc aatagtgcct ttggcaaaca taccaccctc
     2101 cttgaaatta agttttattg atttactata atataacag
//
LOCUS       DSEB01000013            3272 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003400, whole genome shotgun sequence.
ACCESSION   DSEB01000013 DSEB01000000
VERSION     DSEB01000013.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003400"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..941
                     /locus_tag="ENO07_00225"
     CDS             <1..941
                     /locus_tag="ENO07_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDS00419.1"
                     /translation="VIILIGATTENPSFEVISPLLSRCTVFTLSMLAAGDIKILLKRA
                     LKDKERGLGDYPNQVPEEVLDYLAAISDGDARRGLSYLEMLAAHFGEAGAEISIDSVG
                     KILQKKTMLYDKSGEEHYNLISALHKSVRGSDVDASLYWLARMLESGEDPMYLARRLV
                     RMAVEDIGVADPNALTIALNAKEAYHFLGKPEGELALAECLVYLATAPKSNRIYTAFQ
                     KVQQAVRENPSEPVPLHIRNAPTGLMKDLGYGKGYKYAHDYEDAFIDQEYLPDSLKGK
                     KFYTPSDMGFEKKIEERMAWWEKKKKELSRKEQSSE"
     gene            complement(977..1816)
                     /locus_tag="ENO07_00230"
     CDS             complement(977..1816)
                     /locus_tag="ENO07_00230"
                     /inference="COORDINATES: protein motif:HMM:PF02014.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00420.1"
                     /translation="MWVDHVRKFAFVLPLLVTAFFIPQFINAFSAGPPDGKTGAPGEG
                     LCSDCHLNSSGGDGSLSLSGVPAEYTFDQTYTLTVTIEDPDQQRWGFEITASDPLNNA
                     AGVFTITDAVNTQLSDNAAPLKDYVKHTSTGTMLGVQDGPVSWNIDWTAPSSDVGQIT
                     FYLAGNGANGNFANTGDFIYSTSVSAPAPAQECQGICGDANSDESVNVSDAVWIINFV
                     FIGGPGPQPVFACGDANADSSVNVSDSVWIINFVFVGGDPPGTCAPGAWEGQGGDCCE
                     FAI"
     gene            2004..2372
                     /locus_tag="ENO07_00235"
     CDS             2004..2372
                     /locus_tag="ENO07_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00421.1"
                     /translation="MRNFAIISLPGAVIFLFMIAFNCGDKADPFRSVDDPGFKVTYTD
                     QIKVILDNNCVLCHDSEKQGAERNGAPLDVNLDTYENAVAVAERANIQIQAGTMPPTG
                     GLPTELRALFQQWIDQGLEE"
     gene            2373..3269
                     /locus_tag="ENO07_00240"
     CDS             2373..3269
                     /locus_tag="ENO07_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00422.1"
                     /translation="MNAVTRGIIFTLFFITLFMLDRDLHAQEEEPDEIESLETVEIED
                     VTSEWWQNERAYAVLDRAINVMTARTMRMNSFIIIIDHRFRQPVSEEPFHDLLGFDAG
                     GLKVGLGLRYGIYYNLDAGIYRLNGTGEAFDVYEFDAKYRPLRQEKHAFDLALRAGVT
                     WFSQQDLEDATGFFGQLLMNRLFAHRLNIGSGLLYHSESSNEVKSTEDDDFSVAIPAY
                     VEFRISGRFMVNVEAVHSIAGYGSSRPVFSTGIKILTNRHTFAIMVTNTRYISADGIV
                     ANSPNGLDDLALGFYITRELNL"
BASE COUNT          857 a          799 c          831 g          785 t
ORIGIN      
        1 gcgttattat cttgatcggg gcgactaccg agaatccctc cttcgaggta ataagcccac
       61 ttctctcgag gtgcactgtc ttcactctga gtatgctcgc agccggagat atcaagatac
      121 ttctcaagcg cgccctcaaa gataaggagc gcgggctggg tgattatccc aatcaggttc
      181 cggaggaggt gctcgactac ctggctgcaa tctctgacgg cgatgcgcgc cggggcctgt
      241 cttacctgga gatgcttgcc gcgcattttg gcgaagcggg cgcagaaatc agcatcgact
      301 ctgtcggcaa gattctgcag aagaagacca tgctttatga taagtccggc gaggagcact
      361 ataacctgat atccgccctg cataaatccg tacgcggctc cgacgttgat gcttccttat
      421 actggctggc aaggatgctc gagtcgggag aggatcccat gtatctggcc aggaggctgg
      481 tcaggatggc tgtcgaggat ataggtgtag cggatccaaa tgccctgacg attgccctga
      541 atgccaagga ggcataccat ttcctgggca agccggaggg tgagctggcc ctggctgaat
      601 gtctggtcta tcttgccacc gcccctaaat caaatagaat ctacaccgct ttccagaaag
      661 tgcagcaggc ggtcagagag aacccgtccg agccggtacc cctgcatatt cgtaatgcac
      721 ccacgggatt gatgaaagat ctgggctatg gtaagggcta taagtacgct cacgattatg
      781 aagatgcctt tatagatcag gagtatcttc cggacagcct gaaagggaaa aagttctaca
      841 cgccatccga catgggattc gagaaaaaaa tagaggaacg catggcctgg tgggagaaaa
      901 agaaaaaaga actctcccgc aaagaacaat ccagtgaata aattggcccg cttttagtaa
      961 ttcttttgga ataaccttaa atcgcaaatt cacagcaatc gccgccctgg ccttcccatg
     1021 ccccgggagc gcatgttccg gggggatctc cacccacaaa aacgaagttt attatccaga
     1081 cagagtcgga gacattgact gaggaatccg cattggcatc accacatgca aacacgggct
     1141 gagggccggg accgccgatg aaaacaaaat tgattatcca gactgcatcg gagacattca
     1201 cggactcatc actattagcg tcgccgcaga tcccctgaca ctcctgtgcc ggcgcaggag
     1261 cggataccga cgtagaatat atgaaatccc ctgtattggc aaaattacca ttggcgccgt
     1321 tgcccgccag ataaaacgtg atttggccca catcagaaga gggcgcggtc cagtctatat
     1381 tccagctgac cggaccatcc tgcacaccca gcattgttcc ggtggaggta tgcttgacat
     1441 aatcttttaa cggcgcggca ttgtccgata attgtgtatt cactgcatcg gtaatggtga
     1501 ataccccggc ggcattgttc aacggatcgg aagcggtgat ttcgaacccc catcgctgtt
     1561 gatcggggtc ctcaattgta accgtaagag tataggtctg gtcgaacgta tactcagccg
     1621 gcacgccgct cagggacaac gatccatccc ctccgctgct gttaagatgg caatcggagc
     1681 acaatccctc acccggcgcc ccggtcttgc cgtccggcgg tccggccgaa aaggcattga
     1741 tgaattgtgg tataaaaaat gcagtcacaa gcagcggcaa tacaaatgca aatttgcgta
     1801 catgatcaac ccacatatgg ctcccctcgt taaaatgttc tcaaaaatgc tatgaaactt
     1861 tttagaaata caatcagtta taatacaaaa cagaatttat cctgattttg tttctggaca
     1921 aaatgttcca attaatattt atgctcaagg agattgaaaa gggtatgggc ataaggataa
     1981 tcatcagtca atattaaggg cttatgcgga attttgctat tatcagtctg ccgggagcag
     2041 tcatcttctt gtttatgatc gctttcaact gcggtgacaa ggctgatccg ttcagatcgg
     2101 tagatgatcc cggtttcaag gtcacctata cagaccagat caaagtaatc cttgataata
     2161 actgcgtact ctgtcacgat tcagaaaagc agggagcgga acgcaatggg gcgcccctgg
     2221 atgtcaatct cgacacctat gaaaatgctg ttgccgttgc tgaaagagca aatatccaga
     2281 ttcaggccgg aaccatgccg ccaaccggcg gccttccgac cgagttacgt gctcttttcc
     2341 agcaatggat cgatcagggt ctggaggaat agatgaatgc cgtaaccaga gggattatct
     2401 tcacgttgtt ttttatcacc ctttttatgc ttgatcgcga cctgcatgcg caagaggagg
     2461 aacccgacga aattgaatca ctggaaacag ttgagatcga ggatgttacc tcggaatggt
     2521 ggcagaatga aagggcatac gctgttctgg accgagctat caatgtcatg acggccagaa
     2581 caatgcgcat gaattctttt ataataataa tcgatcaccg ttttcggcaa ccagtatcgg
     2641 aagaaccgtt tcatgatttg ctgggtttcg atgccggcgg tctgaaagtg gggctgggat
     2701 tacgatatgg tatctattac aatcttgatg cgggaattta tcgccttaac ggcaccggcg
     2761 aagcctttga tgtgtatgaa tttgatgcaa aataccggcc cttgcgacag gagaagcatg
     2821 ccttcgatct ggcgctgcgc gccggcgtaa cctggttctc acagcaggat cttgaggatg
     2881 ccaccggatt cttcggacag cttttgatga acaggctgtt cgcacatcgg ctgaatatcg
     2941 gcagcgggct gctatatcat tcggagtcct ctaatgaagt caagtcaaca gaggatgacg
     3001 atttttcagt ggcgattccc gcctatgttg agttccgcat cagcggccgt ttcatggtga
     3061 atgtcgaggc agtacacagc atcgcaggat atggctcatc acggcctgta ttctcaactg
     3121 gtatcaagat actgaccaat cggcacacat ttgccatcat ggtgaccaat acaagatata
     3181 tatcagccga tggcattgtt gcgaattcgc ctaatggcct ggatgatctt gcgctcggat
     3241 tttacatcac acgggaactc aatctataga ta
//
LOCUS       DSEB01000014            2014 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1009925, whole genome shotgun sequence.
ACCESSION   DSEB01000014 DSEB01000000
VERSION     DSEB01000014.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1009925"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1626
                     /locus_tag="ENO07_00245"
     CDS             <1..1626
                     /locus_tag="ENO07_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDS00423.1"
                     /translation="LWKWLKGSHNSRPGIILLGIIFFALITFTPAPDRLMSLLSWEKT
                     GKVEQDLNAGYASYRGMHEDESIAEYYSRYSGMGNTVVNEEGNKVKEPLTPSQAAFKA
                     KVMLGILIVAAMFWASGAVPIGITALAVGVFMYFFGILKPDDVAAAYAKDAVIFIFGV
                     LAISNAITKTGLDRRIGLLLLGPAKTLPRLLFIFLPLFGMACSFVSEHALIAFVMPLF
                     MMVYVSSIRAAGVKKDRALAVMFALSLCFAANCGGPGSPAAGGRNAIMLGILSDYGVA
                     PSFGEWVAYGLPFVPVMGLVIAAYFFFMFRRKLVLKNINVSALVKEASDKIGPMNRNE
                     YITAIVLVFLIILWITASDFLGMGGPVILCIVILNLFRILRWKDIAAIPWDVVALYAS
                     ASALGKGLAVSGAALYLADSFISILPEFFRSGEGLAMATSIFTGIATNFMSDGATVSA
                     IGPITIPMAKISGTHPWMVGFATAFASSFAHMMIIGTPNNAIAYTLAKDPATGEQLVR
                     LSDFLKHGAAILVLSFAVLWLWAFFGYWRFIGF"
     gene            1651..>2014
                     /locus_tag="ENO07_00250"
     CDS             1651..>2014
                     /locus_tag="ENO07_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDS00424.1"
                     /translation="MGDKIKLLICDDEVKFLDSIAERLELRGFDVTKATSGREAVEAA
                     RTGGFDLAILDLKMPGMNGIEVLEVLKKEHKYLEVIILTGHGSIDSAVESTKLGAFSY
                     LSKPYDLDDLLKSLRDAYE"
BASE COUNT          484 a          463 c          522 g          545 t
ORIGIN      
        1 ctgtggaaat ggctcaaagg ctcacataat tcaagaccgg gcattatact gctcggaata
       61 atcttttttg cattgataac attcactcct gctccagata gactgatgtc gctattatca
      121 tgggaaaaaa cgggcaaggt tgagcaggac cttaatgcag gatacgccag ctatcgcggc
      181 atgcatgaag acgaaagcat tgctgaatat tacagccgct attcggggat gggtaatact
      241 gttgtcaatg aggaaggcaa caaggtcaaa gaaccattaa ctcccagtca ggcggcattc
      301 aaggccaaag tcatgcttgg catacttatc gtggccgcca tgttctgggc ctcaggagcg
      361 gttcccattg gaattacagc cctcgcggtg ggcgtcttca tgtacttctt tgggatatta
      421 aagcctgatg acgtagcagc tgcctatgcc aaagacgcag ttatatttat attcggtgta
      481 ttggcaattt ctaatgccat taccaaaacc gggctggacc gaagaatcgg gcttctatta
      541 ttggggccgg ccaagacatt gccgcgcctt ctctttattt tcctgcccct tttcggcatg
      601 gcctgctcat ttgtttccga acatgcctta atcgcatttg taatgccgct cttcatgatg
      661 gtctatgtgt cttcaatcag ggcggcggga gtaaaaaaag accgggctct ggcggtcatg
      721 tttgctcttt ccctttgctt tgccgccaat tgcggaggac cgggctcgcc tgctgctggt
      781 ggaagaaatg ccataatgct cggcatcctt tccgattacg gggttgcgcc ctcattcgga
      841 gagtgggtcg cctatgggct tccattcgtt ccggttatgg gattggtaat cgccgcttat
      901 ttcttcttta tgttcaggcg aaagctggtt ttgaaaaaca ttaatgtttc cgcacttgtg
      961 aaagaggcct ccgataaaat cgggccgatg aatagaaatg aatatattac cgcaatagta
     1021 ctggtgtttc taatcatcct ctggataacg gccagtgatt tccttggtat gggcggaccg
     1081 gtgatattat gtatagtaat tctgaatctt ttcaggatct tgcgatggaa ggacatcgct
     1141 gctatcccat gggacgtggt tgccctttac gccagcgcat cagccctggg aaagggactt
     1201 gcggtctcag gggcggcact ctatcttgcg gacagcttta taagcatcct gcccgagttt
     1261 ttccgctcgg gcgagggcct ggcgatggca accagtatct tcacaggtat cgcaacaaac
     1321 ttcatgagtg acggggcgac ggtttctgca atcgggccaa taacaattcc gatggcaaaa
     1381 atttccggta ctcacccctg gatggtcgga tttgccacag cgtttgcatc atcattcgcc
     1441 cacatgatga taattggcac tcccaacaac gctattgcat acaccctggc caaggatccg
     1501 gctactggcg aacagcttgt caggctgagt gattttctta aacacggtgc ggccattctg
     1561 gtgctttcat ttgcagtgct ctggctctgg gcatttttcg gttactggcg ctttatcgga
     1621 ttttagaata taaaataagg aagggataaa atgggcgaca aaattaaact tctgatctgc
     1681 gatgatgagg ttaagtttct tgattcaatc gcagaacggt tagagctgcg cggatttgat
     1741 gtaaccaaag caaccagcgg gagagaggcc gtggaggcgg ccaggaccgg aggcttcgac
     1801 ctggcgattc tggatctgaa aatgcccgga atgaatggca tcgaggtact cgaggtattg
     1861 aagaaagagc acaagtatct cgaggtcata atccttaccg ggcatggttc gatcgattca
     1921 gcggtcgagt ccacaaaact gggcgcattc agctatctct caaaaccata tgatctggat
     1981 gatttattga aatctcttag ggatgcctat gaga
//
LOCUS       DSEB01000015            4860 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001410, whole genome shotgun sequence.
ACCESSION   DSEB01000015 DSEB01000000
VERSION     DSEB01000015.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001410"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            71..379
                     /locus_tag="ENO07_00255"
     CDS             71..379
                     /locus_tag="ENO07_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00425.1"
                     /translation="MDFTSTGSICEENSLAMHACMIATRPALIYWNDTTVRLIKAVKK
                     WREGGLEAYCTIDAGPHVAILGKISDLNKIVSRARNVNGVTTAQKSLPAGGASISECL
                     "
     gene            376..1284
                     /locus_tag="ENO07_00260"
     CDS             376..1284
                     /locus_tag="ENO07_00260"
                     /inference="COORDINATES: protein motif:HMM:PF00288.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00426.1"
                     /translation="MRELKVPGKVMLSGEYAVLHGGTAVMAPVPRYLIIRESNEINSS
                     SLPPVASEALNLDVKEIYDFEDQNGRPGLVLDDSQFYSTDSNGTKVKLGLGLSAAEAV
                     GVIAFRFERAGLSWQDNRDRIIKYAFEVHSRVQGGLGSGADIAACGFAHPIKFRKEND
                     RMHIEPIEGGLRKYKIPLKIAWTGKPSNTRDMVAAFEKWADAGDKELLQEFIASADEL
                     ADAWFQSPLKELFDKIDAFTSNLRQISEAANLSLFLPVHDELEAWARNNGGRAKPTGA
                     GGGDMILLVGDLPARHPEDTLIPLML"
     gene            complement(1296..3305)
                     /gene="tkt"
                     /locus_tag="ENO07_00265"
     CDS             complement(1296..3305)
                     /gene="tkt"
                     /locus_tag="ENO07_00265"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDS00427.1"
                     /translation="MDKIARASEIDQLAINTIRFLSIDAVQKANSGHPGAPMGMAPAI
                     YVLWDRFLKHNPKNPEWFNRDRFILSAGHASMLLYSMLYMTGYDISLDDIRNFRQWGS
                     RTPGHPEYNLSCGIEVTTGPLGQGFANGVGMAVAEKFLAAKFNRPGFDVIDHYIYAMV
                     SDGDLQEGISYEAASFAGHHALGNLIYLYDDNSIQIEGSTSLVFSDDVRKRFEAVNWH
                     VIGPINGNDLTEVEHALREGKEIKNKPTLIICKTNIAYGSPGKQDTASAHGEPLGVEE
                     VVATKKKLGWPLEPDFHVPEKARSHMLESIPRGEKVEQEWNEMFHEYSRKYPEEHAHM
                     IDFIEGRISEGWDEGLDELFDNLESDIPTRGASGKVLNSIAARVENLMGGSADLGPSN
                     KTLLNGESDFSRDNYSGRNLRFGVREHSMTAMANGMALHGGIIPYTGTFLIFSEYMRP
                     AIRLAAMMGLQVIFVFSHDSIGLGEDGPTHQPIEQLTSLRAIPNLTVIRPADPYETMY
                     AWKAAIMNRRGPTAIVTTRQKVPTLRDNHEAARGGYIVWQNSANPQGIIIATGSEVHI
                     SIAAAEKLKSEGIDVRVVSLPSCEIFDDQPQDYRDLVLPPDMNKRLAVEAGASLGWWK
                     YISLEGAVLGIDRFGESAPSKILFERFGFTADNIVVNMKKLLSRS"
     gene            complement(3607..4605)
                     /locus_tag="ENO07_00270"
     CDS             complement(3607..4605)
                     /locus_tag="ENO07_00270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDS00428.1"
                     /translation="MKAMILESPKPIDQKPLVEKELEKPQPQSGQILIRISVCGACHT
                     DLHTVEGELELPKLPIIPGHEIIGNVEKNGPGAERFKEGDRVGVAWLNWACGECVYCR
                     NNQENLCENARFTGLHADGGYAEYTVVDERFAYPIPDIFSDLEAAPLMCAGVIGYRAL
                     RLSEFKPESRLGLYGFGASAHIAIQIARHKNCRVFVFTRSENHRQLARELGAEWTGSA
                     QDMPPEQLDSSVTFAPAGWIVPYALKHLRPGGTLAINAIYMSPIPELEYPLIYKERTL
                     RSVAHVARSDAEEFLPLAAEIPVKTEVEGFELQDANEVLKKMKESRIRGSAALKIR"
BASE COUNT         1108 a         1238 c         1278 g         1236 t
ORIGIN      
        1 cccttatttc aatgcatggg ttactcgagc gagaaaagat tataaactga tgctcgatgc
       61 ggttgagaaa atggatttta catcaaccgg gagtatttgt gaggagaatt cgttggccat
      121 gcacgcctgt atgatagcca cccgtcccgc cctgatttac tggaacgata caaccgtcag
      181 gctgatcaaa gcggtgaaaa aatggcggga gggcggtctg gaggcatatt gtactattga
      241 tgccgggccg catgttgcca tattgggaaa gatttcggat ctgaataaaa tcgtttcccg
      301 cgcgaggaat gtaaatggcg tcacaaccgc acagaagtcg ttgccggccg ggggtgcatc
      361 aatatcggag tgtctgtgag ggaattgaag gtacccggca aagtcatgtt atccggagaa
      421 tatgccgtgc tgcacggggg cactgcggtt atggctcccg taccacgcta tctcataatt
      481 agagagtcaa atgaaataaa cagctcgagc ctgccgcctg ttgcgagtga agcgctaaat
      541 cttgatgtta aagaaatata tgacttcgag gaccagaacg ggcgtccggg attggttttg
      601 gatgacagcc agttttattc cacagactca aatggaacga aggtcaagct tgggctgggt
      661 ctgtcggcgg cagaagctgt gggtgtaata gcgttcagat ttgagagagc aggattatcg
      721 tggcaggata acagggacag gatcatcaag tatgcgtttg aagtgcatag tagggttcag
      781 gggggactgg gcagcggcgc cgatatagca gcctgcggct ttgcacatcc gatcaaattc
      841 agaaaagaaa atgataggat gcatatcgaa ccgatcgagg gtggtctcag gaaatacaaa
      901 attccgctta aaattgcctg gaccggcaag ccctcaaata cccgggatat ggtggcggca
      961 tttgaaaaat gggccgacgc cggagataag gaactgctcc aggagtttat tgcttctgct
     1021 gatgaattgg ctgatgcctg gtttcagtca ccgctgaagg aattatttga taaaatagat
     1081 gcatttactt ctaatctccg gcaaatttct gaagccgcta atctatccct ttttcttcct
     1141 gtccatgatg aactcgaggc ctgggccagg aataatggcg ggcgcgccaa gccgaccggt
     1201 gcgggaggcg gagacatgat attgctggta ggcgatcttc cggcgaggca cccggaggat
     1261 acacttatcc ctctaatgct ctgatttgat aattcctatg atcgagagag cagcttcttc
     1321 atattcacga ctatattgtc agccgtaaat ccgaatctct cgaatagaat ttttgatggc
     1381 gctgattccc cgaaacggtc tatacccagg acagcaccct ccaggctgat atatttccac
     1441 cagcccagcg aggcgcctgc ttccaccgcc aaacgcttgt tcatgtcagg tggaagaaca
     1501 aggtcacgat aatcctgcgg ctggtcatcg aatatttcgc atgagggcag ggagaccacg
     1561 cgtacatcga taccctctga cttcagcttc tcggcggctg caatgctgat atgaacctcg
     1621 gaacctgtgg ctatgatgat cccctgcggg ttagcgctgt tctgccagac aatatatccg
     1681 ccgcgtgcgg cctcgtgatt atcccgcagt gtgggtactt tctgccgggt cgtcacgatt
     1741 gcagttggtc cccggcggtt cattattgcg gctttccagg catacatcgt ttcgtatgga
     1801 tcagcgggcc ggattacggt cagatttggt atcgctctca ggctggtgag ctgctcgatg
     1861 ggctgatgtg tcgggccgtc ctctccgaga ccgatggagt catgcgaaaa gacaaaaatg
     1921 acctgaagtc ccatcatggc ggccaaccgg atggcaggtc tcatatattc agagaatatc
     1981 aggaatgtgc cggtgtatgg gatgattccg ccgtgaagcg ccatcccatt ggccatcgcc
     2041 gtcatggaat gctcgcgcac tccaaagcgc aggttcctgc cggaataatt gtcgcggctg
     2101 aaatcactct ctccattcag gagggtctta ttggagggtc ccaggtcggc cgaaccgccc
     2161 atcaaatttt ccaccctggc tgcgattgaa tttaagactt tgcccgacgc tccacgcgtg
     2221 gggatatcag attcgaggtt atcgaagagt tcatcgagac cctcgtccca tccttcagaa
     2281 attcggccct cgatgaaatc tatcatgtgc gcatgttcct cgggatactt ccggctgtat
     2341 tcatgaaaca tctcattcca ttcctgttcg accttttccc ctctcgggat cgactccagc
     2401 atatgactgc gggccttctc gggcacatga aaatccggtt ccaggggcca ccccagcttc
     2461 ttctttgtgg ccactacttc ttcaactccc aggggctctc catgggcgga ggctgtatcc
     2521 tgtttgccgg gcgagccata ggctatgtta gttttgcaga taatcagggt gggcttattc
     2581 ttgatctctt taccttccct gagggcatgt tcaacttcag tcagatcatt gccgttgatc
     2641 ggcccgatca catgccagtt gaccgcctcg aacctcttcc tgacatcatc ggaaaatacc
     2701 agcgaggtac tgccctcgat ctggatgctg ttgtcgtcat agagatagat taaattgccg
     2761 agggcgtgat gtccggcgaa gctggccgct tcataggaga tgccctcctg caggtcgccg
     2821 tcggagacca tggcatagat ataatgatcg atcacatcaa agccggggcg gttgaatttg
     2881 gccgccagaa acttctcggc gacagccatc cccacaccgt ttgcaaatcc ctgcccgagc
     2941 ggcccggtgg taacctcgat gccgcaggat agattgtatt cagggtgacc cggcgtacgg
     3001 cttccccatt ggcggaaatt cctgatgtca tccagggata tatcataacc ggtcatatat
     3061 agcatgctgt aaagcagcat ggaagcgtgc cctgccgaaa ggatgaagcg gtcgcgattg
     3121 aaccattcgg gatttttggg attatgcttc aggaacctgt cccagagaac gtagattgcc
     3181 ggggccatgc ccattggcgc tccggggtgt cccgaattag ctttctgcac agcatcgatg
     3241 gacaggaacc tgatggtatt aatggcgagt tgatcgattt cgctcgccct ggcgatctta
     3301 tccattttca tcagctctcc cgagccggtt tccaagtctt cggtctgacc tctgtaatcc
     3361 ataatcccct ctacgatggt ttttgggtgt aatgaggatg gtgaagatag ttctaagatt
     3421 tactgcgcca gttatgaagt acgacacgcg tttgaccatc ctgcggcttc taacttattc
     3481 ccttgtatgc cttctacacg cgccctcaga atggcagagc tgttctctgc agagaatcat
     3541 tattaactgt tttatggctt ttgtcaacat ttttagggca gataattgcc gttacgggtc
     3601 aggatttcag cggattttga gcgctgccga gccgcgaatc cggctctcct tcattttttt
     3661 caagacctcg ttcgcatcct gtaactcaaa gccttccacc tctgttttca ccgggatctc
     3721 ggctgccagc ggcaggaatt cctccgcatc tgagcgggca acatgcgcca cgctgcgcaa
     3781 cgttctttcc ttatatataa gcggatattc aagctcgggt atcgggctca tgtatatggc
     3841 atttatagcg agtgtgcctc ccggtcgaag gtgcttcagg gcatacggaa caatccatcc
     3901 tgccggggca aatgttaccg agctgtcgag ctgttccggg ggcatgtcct gtgcgctgcc
     3961 ggtccattcc gcgccgagtt cccgggcaag ctgcctgtga ttttcactgc gggtaaaaac
     4021 gaaaactctg caattcttgt gtcgggcaat ctgaatggcg atatgagcgc tggcgccgaa
     4081 gccataaagt cccagccgtg attccggttt gaattcgctc aggcgcagtg cacggtagcc
     4141 gattactccc gcacacataa gcggggcagc ctccaggtcg gagaaaatgt ccggtatcgg
     4201 ataggcgaag cgctcatcca caaccgtgta ctcggcatag cccccgtcag catgaaggcc
     4261 ggtaaacctg gcgttttcgc acagattttc ctggttgttc ctgcaataga cacattcccc
     4321 gcaggcccaa ttcagccagg ctaccccaac ccggtcgcct tctttgaaac gctctgcccc
     4381 gggcccgttc ttttcgacat ttccaattat ctcatgtccc ggaataatgg gcagcttcgg
     4441 caactccagc tcgccttcta ccgtgtgcag gtctgtatgg caggcgccgc aaaccgagat
     4501 gcgaatcagg atctggcccg attgcggctg gggcttttcc agctcttttt caaccagggg
     4561 cttttgatct atgggttttg gagattccag tatcatggct ttcatagttc atactccgtc
     4621 tcaatattta tttgaaggtc ttaaagatca tcgtatacag atatagaaac taagtataag
     4681 aggaattttg gagattacca ataataaaaa agccccatcc gaacaggatg gggctttgta
     4741 tctatctatc tgtaatgcga tttattcacc gcagtcgggg gtaggacctc cgacaaagac
     4801 gtagttgatg atataaacag catcactgac attaaccgta ttgtcgcagt tggcgtcgcc
//
LOCUS       DSEB01000016            1701 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1014468, whole genome shotgun sequence.
ACCESSION   DSEB01000016 DSEB01000000
VERSION     DSEB01000016.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1014468"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(159..1340)
                     /locus_tag="ENO07_00275"
     CDS             complement(159..1340)
                     /locus_tag="ENO07_00275"
                     /inference="COORDINATES: protein motif:HMM:PF13187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00429.1"
                     /translation="MQAFARKRDGRNIEVFIVKKLQEPDLDFIRYVKKAGGADLNKCY
                     QCATCSVVCELSPDHKPFPRKEMIWASWGQKERLIKDPDVWLCHQCNDCTVHCPRGAR
                     PGDVLAAVRSYVFSENSYPRFMGRALASPKALPILLLVPILVIGAIMLGFADFEAVDM
                     GLGAAVDYNNFIPHGWIEGLFITGNVLILAFAAVGLIRFWRGLKETFPDQEVPGFIPS
                     AILAVKEIITHENFNKCVTNKARAYAHFMVLFGFLGSMATAGLALLWTVVLGHHSPID
                     LPNLIKVVGSVSGLMLLIGLGIIFVRRFADRDRVGANGYSDWLFLYVLAIVGFTGLIL
                     QFLRMGGLAAAAYITYFIHLVFVFFLLWYAPYSKFAHMFYRTLAMIYARGINRRPKSL
                     R"
BASE COUNT          467 a          424 c          399 g          411 t
ORIGIN      
        1 actttgaaaa agtgcattat ttcacataat attgatagaa cttataatgc ccctgcgtga
       61 ccatattgga gggtgttggg gtttttatgt gttccggcag agcatatcgt tgaggaaaaa
      121 aagccggaga atcccccggc catttttttc aaggtaattt atctcagaga tttcggtctc
      181 ctgtttatgc ctctggcata gatcatcgcc agcgttctat agaacatatg cgcaaatttc
      241 gaatagggag cataccataa aaggaagaat acaaaaacaa gatggataaa ataggtgata
      301 taggctgctg ctgccaaccc gcccatcctc agaaactgca ggatcagtcc ggtaaaaccg
      361 actatcgcca ggacatacag gaagagccaa tcggagtagc catttgcgcc gactcgatcc
      421 ctgtcggcaa agcgcctgac aaaaataatt cccagcccaa tcagaagcat caggccgctg
      481 actgagccaa ccaccttgat caggttggga agatcgattg gggaatgatg ccccagcacc
      541 accgtccata ataatgccag accggctgtc gccattgaac caagaaatcc gaacaggacc
      601 atgaaatggg catatgctct ggccttattg gttacacatt tattgaagtt ttcatgcgtg
      661 attatttcct ttacggccag aattgcgctg ggtataaatc ccgggacctc ttgatcggga
      721 aatgtctcct tcaaccctct ccagaaacgg atcagcccta ccgcagcgaa tgccagtata
      781 agaacgttgc cggttataaa caatccctcg atccagccat gcggaatgaa attattataa
      841 tccactgccg cgcccaggcc catatcaacg gcttcaaaat ctgcaaaacc cagcataatt
      901 gcgccaatta cgagaattgg tacaaggaga agaatcggca aggcctttgg tgaagccagc
      961 gcccttccca taaacctggg atatgaattc tcgctgaata catatgatcg tacagccgca
     1021 agaacatccc cgggacgagc tccccgcggg caatggactg tacagtcatt acattgatga
     1081 cacagccaga catccgggtc cttgatcagg cgctctttct ggccccagga tgcccagatc
     1141 atctccttgc gcgggaacgg tttgtggtcc ggtgagagtt cacatacgac cgagcaggtc
     1201 gcacattgat agcatttatt gagatcagct ccgcccgctt tcttcacgta gcggataaaa
     1261 tccagatctg gttcctgtaa ctttttcacg atgaacacct ctatgtttct gccatctctt
     1321 ttgcgagcaa aagcctgcat gctcgcaagc agttatcata gattttacgg tattcaatgt
     1381 caaggatttt gttaattttt tcacgaactt tttattttca gaccgatacc gaagatttct
     1441 gccggggata acaaaaaaag agcggaagaa cgctcttccg ctcaggtgat tatcatatgg
     1501 gcggctgcct tagaattgac agcagtttcc gccgggccat gatccgggtg agcaattgtt
     1561 aggaggatca ccgccaacga aggcgtagtt gatgacccag accgcatcgg atacgttaac
     1621 ggatgcgtct gagttggcat caccgcaggc cgggatgggc tgcggaggat ctccgcctac
     1681 aaaggcgaag ttaatgatcc a
//
LOCUS       DSEB01000017            3055 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003962, whole genome shotgun sequence.
ACCESSION   DSEB01000017 DSEB01000000
VERSION     DSEB01000017.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003962"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            1441..2361
                     /locus_tag="ENO07_00280"
     CDS             1441..2361
                     /locus_tag="ENO07_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00430.1"
                     /translation="MRRLIYTSFIMLLLLCVSAYSADIAVESSLDRNKAYIGDRIGYS
                     LIIEADSAIRIDSLEIPEDLGEFEIKNWLLESERVRKGVRKMEYSGILTVFETGKVMI
                     PPLPIVYHPSDDVTDTIYTDSMDVFIMSLVLDDSAADIRDLKGVKSLGENRALIIIII
                     VLLLAIAAGLMWYFFRKKPEMEAQKTEPLKSPWDEALERLARLRENNLDPKDYYIELS
                     DTIRRYVERRYGFSATDMTTYEIKQEIPKLSIEDELNINLSDLLDNSDLVKFARFMPD
                     KSLIEEDFQRAWVFVNKTKPGTPVSEEVSS"
     gene            2358..>3055
                     /locus_tag="ENO07_00285"
     CDS             2358..>3055
                     /locus_tag="ENO07_00285"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00092.26,HMM:PF07584.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDS00431.1"
                     /translation="MSFELGSPSYLYLLILIPILLWFRYLRRKKVHAPVIYSDTGLVT
                     RVRYRGGLWPRYISPILKSLALIAIIFALARPRFARTFEEVKTQGIDIILTLDVSGSM
                     KAEDFKPQNRLYVAKEVIKEFVQERTMDRLGLVVFAAQAFTQCPLTLDHNVLINFLDN
                     VDFGMVEDGTAIGTAIANSCNRLKDSPSKSKVIVLLTDGVNNRGKIDPLTAADIAASL
                     GIKIYTIGIGKPGNA"
BASE COUNT          724 a          681 c          771 g          879 t
ORIGIN      
        1 attatggggc tggccatgat atttcttcgg attgtggatg gcgatgaggg tttttatcta
       61 tccgccgcta cgctgcttgc ccagggcggg gagccgtatc tcgacttctt ctttccgcag
      121 atgcccggac tgcccgtttt tctggcgcct ttcagcggac atggctggac cacactattt
      181 ttggcccggg cctttggatt tatctgccat ctgatcctgg cgcttttgat ctatagctat
      241 tccaagaaca ttattgacaa tcgcgccctt aatctgagcc ttttcttttt ggccgccctc
      301 tccggaccgc ttctgacatg gaacagcctg gccaagccgt atcccctaag caatcttttc
      361 ttcttctcat cattcgtgat tttgacactg accatcaagc agcgtaaaat gaattacctg
      421 atgatatttc tggcctttgc cttcctggcg ctggcaatca acatacgggc aatatttctg
      481 atcttcgcgc cgctctattt ctttctttta tatttctact tccgcagtat tcgggagatt
      541 cgctttcctt attatctttt gacggtattc ctgggactgg tgcttccatc tttatatgct
      601 atcaggctga tgtttatctc cccggctaat tttcttttca ataatctcgg atttcacctt
      661 ctcagggccg aggaccccgg atttgtaaat atcattttaa tcaaactttt tgtactggct
      721 aaagtactga ttcagccgca ctattttatt cctttagggc tggctttctg gtcacttgtg
      781 atcatcaatt caggcaaaca caaatatgat tacggtccct ggcaaaggat ggtctttcaa
      841 ctatctatgg tggtggcggc ctttatattt gtggtctata tgattcccca tccggtacat
      901 gtgcaatatt tcaatcagac attgccgttc ctgattattg cctctatacc tgcgatcaag
      961 ctggtacttg agaaatccgg ttctgtaaag ccggttctgg cggcggttta tctgctgggt
     1021 atcttgttat acccggcgct ctatatcctg gatattcggg acaaatattc tgactacaaa
     1081 atagcgaatg tcaagagggt gacacaggca atacggcaca attcgatgcc cgaggacccg
     1141 atactctctg aatgggtggc ctataatgtt ctttctgaga gagcgcaact ggccggagga
     1201 gaatttgtcg gccatgagta tgtgttctcc tcttcgattg tgccgcgcag taaatatcat
     1261 ttgctctcgg acgagaggat caatgctttc ttgcacaatt ccgtgccgga gcttgtagta
     1321 ataaagaact ctccaattga aacctgggag aaggaactgg ataaaaacta caagataatg
     1381 gtaaaaataa ataacgtgtt tatttatgat cggaataaag ctgggcaggg cgaataagag
     1441 atgagaaggc tgatttatac atcatttatt atgctgttat tgctgtgtgt ctccgcttat
     1501 tcagcggata ttgcagttga atcatctctg gatagaaata aggcctatat cggcgacagg
     1561 ataggctatt ccctgataat cgaggccgac agtgcaatca ggattgattc gctggaaatt
     1621 cctgaggatc tgggtgaatt tgagataaaa aactggctgc tggaaagcga gcgggtcagg
     1681 aagggcgtca ggaagatgga atattccggt attttgactg tctttgagac gggcaaagtc
     1741 atgattccgc ctctgccgat tgtatatcat ccctcggatg atgtcaccga tacgatttat
     1801 acagattcca tggatgtttt tatcatgagc ctggtgctgg acgattcggc tgcggatata
     1861 cgtgatctca agggagtcaa gagcctgggg gagaatcgtg ccctgattat tataattata
     1921 gtgcttttgc tggcaattgc ggcggggttg atgtggtatt tcttcaggaa aaagccggag
     1981 atggaggccc agaagaccga accgcttaag agtccctggg atgaggctct ggaacgattg
     2041 gcgcggttgc gggaaaacaa tctggatcca aaagattact atattgagct gtcggatacg
     2101 atcaggcgct atgtggaacg ccgttacggt ttttcggcta cggatatgac aacgtacgag
     2161 atcaaacagg aaattccgaa actcagcatt gaagatgagc tgaacattaa tttgtccgat
     2221 ctgctggata attccgacct ggtgaaattt gccaggttca tgccggataa gagccttata
     2281 gaggaggatt ttcagagggc ctgggttttt gtgaacaaaa ctaaacccgg tacaccggtt
     2341 tcagaggagg tgagctcatg agtttcgaat taggctcccc ctcgtatcta tacctcctga
     2401 ttctgatccc gattttgctc tggttcaggt atctgcgccg aaaaaaggtg catgctccgg
     2461 tcatatactc agacactggc cttgtcacgc gtgtccggta tcgaggggga ctatggcccc
     2521 gatatatctc tccgattctt aagagcctgg ccctgatcgc gataattttt gccctggcgc
     2581 gccctcgatt tgcgcgcaca ttcgaggagg tgaagacaca gggaattgat ataatattga
     2641 ctctggatgt gtcggggagc atgaaagccg aggatttcaa gccgcagaac aggctttatg
     2701 tggccaaaga agtgatcaag gagttcgtgc aggaaaggac gatggatcgc ctgggtctgg
     2761 tcgtgtttgc cgctcaggca tttacgcagt gcccgctgac attggatcat aatgtcctga
     2821 tcaattttct ggataatgtg gactttggca tggtggagga cggtactgcg atcggtaccg
     2881 ccattgcgaa cagttgtaac cgcctgaaag actccccctc gaaaagcaag gtgattgtgc
     2941 ttttaaccga cggagttaac aaccgcggca agatcgatcc gctgacggca gctgatatcg
     3001 cggcctccct gggcatcaag atttacacta tcgggatcgg caagccgggc aatgc
//
LOCUS       DSEB01000018            7238 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000597, whole genome shotgun sequence.
ACCESSION   DSEB01000018 DSEB01000000
VERSION     DSEB01000018.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7238)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7238)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7238
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000597"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..432
                     /locus_tag="ENO07_00290"
     CDS             <1..432
                     /locus_tag="ENO07_00290"
                     /inference="COORDINATES: protein motif:HMM:PF02954.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HDS00432.1"
                     /translation="VITVELPPLRARKEDIPLLVHHFINRFNNLKDRLITEVSPDVMA
                     VLMNYDYPGNIRELENIIEHAFVLSKGTVINLECLPDSIRPKDKSTEIVSHSLEEMEA
                     AYLKEALRKNNWNRTKTAAQLGIHKTTLWRKMKKLGVEIPE"
     gene            612..1022
                     /locus_tag="ENO07_00295"
     CDS             612..1022
                     /locus_tag="ENO07_00295"
                     /inference="COORDINATES: protein motif:HMM:PF02579.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dinitrogenase iron-molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HDS00433.1"
                     /translation="MRIAIPVWNGRVSPVMDTARRIMIVDQDSNNPGDRQMIDLSGRH
                     PHEIIKTLEMMNIDILICGAISAQLETQLRGINIKVYPWIRGNVELILGAYSRGALDN
                     EELQLPGCRGRGRGRGFKRRHGMQRNFGKFKDER"
     gene            1096..1341
                     /locus_tag="ENO07_00300"
     CDS             1096..1341
                     /locus_tag="ENO07_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00434.1"
                     /translation="MMFSNGSGGGGGRGQGGGKGGGSGDRQGRGQGQGRGRMGGRGQG
                     PGGQCVCPNCGEIASHKRGVPCYDIKCPKCGELMTRK"
     gene            1314..1757
                     /locus_tag="ENO07_00305"
     CDS             1314..1757
                     /locus_tag="ENO07_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDS00435.1"
                     /translation="MRGTDDTQVVKSKGVSLLKIAIPVERGMLSPHFGHCTEFAFIDA
                     DPVSKKIMRTEIVSAPPHQPGLLPQWLGETGCNIIIAGGMGNRAINLFNQRGIEVICG
                     APSQSPQALALEFLNGELQTCGNLCDEPGFKEAGGRNCKNHDKGL"
     gene            1975..3882
                     /locus_tag="ENO07_00310"
     CDS             1975..3882
                     /locus_tag="ENO07_00310"
                     /inference="COORDINATES: protein motif:HMM:PF00724.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDS00436.1"
                     /translation="METFSNLKIGSVQIPNRLIMAPLKTGLGSPSGEANEKHLAFYQR
                     RAEGGTGAITLEPLYVDLRGREHPLQLGLTADSYIPGLKAITDVIHMNGSVAIAHLNH
                     AGRAANPQASGSPPEAPSPVMCPARGNVPEEMSAERIKVVVCQFGEAARRAREAGFDI
                     VELQFGLGYLVAQFFSPHTNLRKDEYAAKGDGRYRFADQILDSVREKAGEDFPIMARI
                     SATEIIEDGLNIHDAIELTHFLEERRVNALHVVSGSACDSGPWYFQHMRLPLGKNLKW
                     AAEIKEKTSLPVIVAGRMGMPDLIRQVLEEEMVDAVALGRPLVAQPDFAIKMKEGRDD
                     EIIQCGACLQGCLARLKSGHEIGCLINPEAGREYQRIAQPERSKKVVVVGGGPAGMQA
                     ALTASSRGHKVVLFDRKKLGGQIHLAYVPPGKEMMEKPLMGLVNKVKKSPIELHIPRK
                     ATVDLIAEENPDVVMLASGAKPVLPAIPGLQNVLFAEDVLTGKSIPGKRVLIIGGGMV
                     GVELAEYLASRKHEVTIVEILDQLAGDMEPLTRKLTLKMIDAMGVKIFTRTNISWFEE
                     NKAYVMEKEGDVYLGEFDSVIAAMGMRSTIELEAELRERGFDVRVIGDARRPGQIFDA
                     VRDGYDAAISI"
     gene            4089..5771
                     /locus_tag="ENO07_00315"
     CDS             4089..5771
                     /locus_tag="ENO07_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS C-terminal target domain-containing
                     protein"
                     /protein_id="HDS00437.1"
                     /translation="MKRVILTLLVLAVLPAILFAQTSEAGKPIMDLYDLDINPGDSVH
                     LDSSHVWILKDGVFVDSGAVLSIDPGTIIKGEPGTGANSKYLCVARGGRIRAEGTENK
                     PIVFTGDVDNVDDPYDIAYGTNGLWGGVLILGRSFCNTPTGTQQIEGIPSEETRGEYG
                     GGANPDSLDNSGIFRYVSIRHGGTEIGAANEINGLTMGAVGAGTVIEYVEVYNNYDDG
                     FEWFGGTVNTKYLVSAFNGDDAYDYDEGFRGYHQFWFVIQDSASGNRCGEHDGGTGDE
                     QGTPYAEPYIFNVTYIGGGSNTANADNDHALKIRDNAGAHYANGIIGYFGGDVVDFED
                     LCGPDGTPHDTRTRLNAGDLTLENMFFWDFGDGSDPVVLFPPESYDCNGDEIEDSTSD
                     FAFVYMTDPANNNSWTVDPMLLNVSRNQVWHALDPRLDPASPANTPGQPEPSDPHFDD
                     VDYHGAFPPAGNKNSLEGLWISNWTYLWQRAHVNLLCGDANDDGSVNISDAVTIINFV
                     FIGGKSPYPYFVADSNCDCSVNVSDAVVVINYVFIGGDSPCQLPGCPPTCVF"
     gene            6341..>7238
                     /locus_tag="ENO07_00320"
     CDS             6341..>7238
                     /locus_tag="ENO07_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00438.1"
                     /translation="MLKKLSVILFLAIFVLSMTAAARDLDVKAGLTNNEAGREAKLFA
                     DGRDEAQVMSMVKPYGTQSIAQTFAPTRQFYSNEITIRDMWHNTAPGRNIFYGGYYNA
                     GCDYVYMNFPDRYFIGSSPYSAVRGAIFDWSVGGWDFTVGGGLQLFGIIPSYYPNQSA
                     NPKDGQGVIGFHSFPGAGTSGWSHAGFAGPCPSDMYTYVSDSMPGPPNTANIQTGYCG
                     DTDANQNNGPYIWPHIDVDTNSAGEMVIHAAANEAVCATAPTEAIETSSLLYYRKVGS
                     NPWEGPIFMDSAYLISQLVVADK"
BASE COUNT         1857 a         1658 c         1918 g         1805 t
ORIGIN      
        1 gtgataaccg tcgagcttcc ccctttgcgc gcacgaaaag aggatatccc gcttcttgtg
       61 caccatttca tcaatcgctt caacaatctc aaggataggc tcattactga ggtctcgcct
      121 gacgttatgg cggttttgat gaattatgat taccccggaa atatccgtga actggaaaat
      181 ataattgagc atgcttttgt actctccaaa ggtactgtca taaacctgga atgcctgccc
      241 gatagtatcc gtcccaaaga taaaagcaca gagattgtct cccattcgct ggaagaaatg
      301 gaggccgcct atctcaaaga ggcgcttcgt aagaacaact ggaatcgtac gaaaaccgcc
      361 gctcagctcg ggattcataa aacgaccctc tggcgcaaaa tgaaaaagct gggtgtggaa
      421 attccggaat agtgttgcgt aaatgcaata ataatattag aaaagactgc acatatgcac
      481 taatcttaca gcccagttgc aacgctctac tccttaaccg atcttatatt ttgttgtctt
      541 ataaagcatt atagaattgc atcagagtat ttattaaatt cggcatcgag actgctctat
      601 ataacatagc tatgagaatt gcaatacctg tatggaatgg ccgcgtttcc cctgttatgg
      661 ataccgcgcg cagaattatg atagttgacc aggattcaaa taatccaggt gacaggcaga
      721 tgatcgattt aagcggtcgg catccgcatg agatcatcaa gaccctggag atgatgaata
      781 ttgatatcct tatctgcggg gcgatctcag cgcaactgga aacccagtta cgcggcatta
      841 atatcaaggt ctatccctgg attcggggca atgtcgagct gatcctggga gcttattccc
      901 ggggagcgct ggacaatgag gagctccaac tgcccggctg ccggggcaga ggcagggggc
      961 ggggattcaa acgcagacat ggaatgcagc gaaatttcgg caaattcaaa gatgagaggt
     1021 gagagcaatc aatttagcag gacaaagtct aacttgataa ctgaaaatcg aagaagaaag
     1081 agagatggtg attttatgat gttttcaaat ggttcaggcg gcggcggagg ccgtggacaa
     1141 ggcgggggca aaggcggcgg atccggcgac aggcagggcc gggggcaggg tcagggccgg
     1201 ggccggatgg gcggtcgcgg acaggggccg ggaggccagt gtgtttgtcc gaattgcgga
     1261 gaaatcgcat cccacaaaag aggagttcca tgttatgaca taaaatgccc caaatgcggg
     1321 gaactgatga cacgcaagta gtgaaatcga aaggagtatc cttgttaaaa atcgcaatac
     1381 ctgttgaacg ggggatgttg agcccccatt ttgggcattg taccgagttc gcatttatcg
     1441 atgcggaccc ggtgagcaag aagattatga gaacagagat agtctctgca cctcctcatc
     1501 aacccgggct tctgcctcaa tggctcggtg aaaccggctg caatattatc atcgcgggag
     1561 gcatgggtaa tcgtgccata aatctcttca accagagagg tattgaggtg atctgcggag
     1621 cgccgtcaca gagtccgcag gctctcgcac tggaatttct taatggagaa ctccagacct
     1681 gcggaaatct gtgtgatgaa cccggtttca aagaggcggg aggccgtaac tgcaagaatc
     1741 atgacaaggg tttgtgaccc tcgctgagcg cgcggcaggt ttccttacag aagataaaat
     1801 gatctgccat tgacctgccg cgcatttctt ttattggatt tttgattttc aacgttcggg
     1861 atgattttcg agcggctttg caaatatttg attgaaatct ggtcaattaa agcttacttt
     1921 aggtttgata atcatcctct caaaaacact ctctgaatct tatgggagag acaagtggaa
     1981 acattttcaa atctgaaaat tggctcagtc cagattccca acagactgat tatggctccc
     2041 ctgaagaccg ggcttggaag tccctccggt gaggccaacg aaaagcatct ggccttctat
     2101 cagcgccggg cagagggcgg aacaggagcc ataacattag aaccccttta tgtcgacctg
     2161 cgtggacgcg aacatccgct ccagttgggc ctgacggcag acagctatat acccggcctc
     2221 aaagcgataa ctgacgttat tcatatgaat ggctcagtgg ctatcgccca tttaaaccat
     2281 gccggacggg cggccaatcc ccaggcttcg ggttcacctc ccgaggctcc ctcacccgta
     2341 atgtgtcctg cccggggaaa tgttccggag gagatgagcg cggagcgaat aaaagtggtc
     2401 gtatgccaat tcggcgaggc cgcacgcagg gcacgggaag ccggatttga tattgtagaa
     2461 ctccagtttg ggttgggcta tctggtcgcg caattcttct caccccacac gaatttgaga
     2521 aaagatgagt acgctgctaa gggagacggg agatatcgct ttgcagacca gattcttgat
     2581 tcagtcaggg agaaggcagg tgaagatttt ccaataatgg cgcggatatc ggcaacagaa
     2641 attattgagg acggcttgaa tatccatgat gccatcgagc tgacccattt tctggaagag
     2701 cgtagagtca atgctttgca tgtggtcagc ggctcggcct gtgattcggg accatggtat
     2761 ttccagcaca tgcgtctgcc cctgggcaaa aatctcaagt gggccgccga aatcaaagaa
     2821 aagaccagcc ttcctgttat tgttgcgggc aggatgggga tgcccgattt gattcggcag
     2881 gtcttggagg aggagatggt tgatgcggtt gccctggggc gtccgcttgt agcccagccg
     2941 gattttgcga tcaagatgaa ggaaggaagg gatgatgaga tcatccagtg cggggcatgt
     3001 ctgcagggct gtctggcgcg cttgaaatcg ggccatgaga tcggctgttt gattaatccc
     3061 gaagccggga gagaatacca gagaattgcc cagcctgaaa ggtccaaaaa agtcgtggtc
     3121 gtgggcggag gtccggcagg aatgcaggcg gccctgaccg cttcctcgag gggccacaag
     3181 gtggtacttt ttgaccgcaa gaaactcggc gggcaaattc acctggccta tgtgcctccc
     3241 ggtaaagaaa tgatggagaa gccgcttatg ggactggtca acaaggtcaa aaagagcccc
     3301 atcgaattgc atatcccgcg caaagccaca gtcgatctga ttgccgagga gaatcccgac
     3361 gtggtcatgc tggcttccgg tgccaaacct gttcttccgg ccattccagg actgcagaat
     3421 gtcctttttg cagaggatgt gttgacaggt aagtccattc ccggaaagag agtccttatt
     3481 atcgggggcg gtatggtggg ggtcgaattg gcggaatatc ttgcttccag aaagcatgag
     3541 gttactattg tcgaaatcct ggatcaactg gcaggtgata tggagcctct cacccgcaag
     3601 ctgaccttga agatgatcga tgcgatgggg gtcaaaatat ttaccaggac aaatataagc
     3661 tggtttgaag agaacaaagc ttacgtcatg gaaaaagagg gggatgtgta cctgggagag
     3721 ttcgattctg tgattgccgc tatgggtatg cgatccacaa ttgagctcga ggcagaattg
     3781 cgcgagcgcg gttttgatgt gcgcgtaatt ggtgatgccc gccgcccggg ccagattttt
     3841 gacgctgtcc gcgacggcta tgatgccgcg atcagcatct gaaagtttac actgattcca
     3901 cacattccgc aaaaaatctt ctaataatca tcagacattt caaacatttt cctgaagata
     3961 atctaattct cttatcattt cattctaaca cttcacaacc atcttcacag cgtcctgcgg
     4021 attacaggac aaaaaataaa agcaataagg gtaatgagcc tgacaaaaaa actttcagga
     4081 ggttttaagt gaagcgtgtc attctaactt tactcgttct tgcggtcttg cctgccattc
     4141 tttttgcgca gaccagcgaa gccggtaagc cgatcatgga tctctatgat cttgatatca
     4201 atcccggcga ttcagttcat cttgacagca gccatgtgtg gattctcaaa gacggcgttt
     4261 ttgtcgattc cggagctgtt ctcagtattg atcccggcac catcatcaaa ggagagccgg
     4321 gaaccggcgc taattccaag tatctgtgtg tcgcccgcgg gggcaggatc agggcggaag
     4381 gaaccgagaa caagccgatt gttttcaccg gtgatgtgga taatgtcgac gacccgtatg
     4441 atattgctta tggcaccaat ggcctctggg gaggtgtgtt gattcttggc aggtcttttt
     4501 gcaatacccc tacaggcact cagcagatcg agggtattcc cagcgaagag actcgtggag
     4561 aatatggagg tggagcaaat cctgatagtc tggataattc cggtatcttc agatacgtct
     4621 ccatccgtca cggcggaaca gagatcggcg ccgccaatga gattaacggt ctgactatgg
     4681 gtgctgtcgg tgcgggtact gtgattgaat atgtggaagt ttacaataac tatgatgatg
     4741 gttttgagtg gttcggcgga acagtcaaca ccaaatatct ggtctcagct tttaatggtg
     4801 acgatgccta tgactatgac gaaggcttcc gaggctatca tcagttctgg ttcgttattc
     4861 aggactccgc cagtggcaat cgttgcgggg agcatgacgg cggtaccggg gatgagcagg
     4921 gaacaccgta tgccgagcct tatatcttca atgtaaccta tatcggcggc ggttccaata
     4981 ctgcaaatgc ggacaatgac catgctctga agatacgtga caatgctggt gctcactatg
     5041 ccaacggtat tatcgggtac tttggaggag atgttgttga ttttgaagat ctttgcggac
     5101 ccgacggcac tccccacgat acccgtaccc gtctgaatgc cggcgatcta acgcttgaaa
     5161 acatgttctt ctgggatttc ggggacggtt ccgatccggt tgttctattt ccgccggaat
     5221 cctacgactg taacggtgat gagattgaag actccaccag tgatttcgct ttcgtctata
     5281 tgaccgaccc ggcaaacaac aattcctgga ctgtcgatcc gatgctcctc aatgtaagcc
     5341 gaaaccaggt ctggcatgct cttgatccgc gtctcgatcc agccagcccg gccaataccc
     5401 cgggccagcc ggagccaagc gatccccatt ttgacgatgt agattatcat ggtgcttttc
     5461 ctccggccgg aaacaaaaac agcctggagg gactgtggat ttccaactgg acctatcttt
     5521 ggcagagagc gcacgttaat ctcctttgcg gtgatgctaa tgatgacgga tcagttaaca
     5581 tatccgatgc agttacaata ataaacttcg tatttattgg tgggaaatct ccgtatccgt
     5641 actttgttgc ggattccaat tgtgattgtt ctgtcaatgt atcagacgcg gtcgttgtga
     5701 ttaattatgt ctttattggc ggcgattcac cctgccagct gccaggctgc ccgccgactt
     5761 gtgttttcta aatggagatc aaaaaatata gtttttctcg gcccgcagtt aatcgtgcgg
     5821 gtcttttttt ataatttgaa aaaaatgtga caaacaatac ccataatagc gctgcaaaat
     5881 atgagaaaaa tcccgggata tccagcttct caatagtact atcaaggaca aaatgaactt
     5941 gacaagaaaa cctgaaaatt gtattttatt tttagccaat tccgggtgga aatttcttca
     6001 acttggtatc acatcgagct cacacccagg aaaacggaag gctccccggc agttggacaa
     6061 gcaaaaataa gaagcaaatt caattacgaa taccgccgtt ttttaaggga atcgctgcat
     6121 ttacttgcgc gctccgcctt ttacttttgc gggacagcat catatgcagt aaagtggact
     6181 gacaggacca aaagtaaaat ataagtcggc acgaaaaaat atatttgaga ggaggtgatt
     6241 ggtcacaatt aatttggccg aagtctatat tgcagaggac tttggcattc ttttcagttt
     6301 aacttgtctt ttctacaaga attcacgaaa ggagcgtgag atgctgaaga agctttcagt
     6361 tattcttttc cttgcaattt ttgtccttag catgactgct gcggccaggg atctcgatgt
     6421 caaggctggg ctgaccaaca acgaggctgg tcgtgaggcc aagctgtttg ccgacggtcg
     6481 tgatgaggcg caggtcatga gtatggtcaa gccctatggc acacaatcca tagctcagac
     6541 ctttgcgccg acaaggcagt tttacagcaa tgagatcacg attcgagata tgtggcacaa
     6601 taccgcgccc ggccgtaata ttttctatgg tggttactat aatgccggat gcgactatgt
     6661 ttacatgaac ttcccggatc gctattttat cggttcgagc ccttacagcg cggtcagggg
     6721 agctatcttt gactggtcag tgggaggctg ggattttacg gtaggcggcg gccttcagct
     6781 ttttggtatt attcccagtt actatcctaa ccagagcgcc aatcccaagg acggccaggg
     6841 agttataggt tttcatagtt tccccggcgc gggtacatcg ggctggtccc atgcgggttt
     6901 cgcaggtccc tgcccgtccg atatgtacac ttatgtttcg gacagcatgc ctggccctcc
     6961 gaacactgcc aatatccaga ccggctattg cggtgatacg gatgcgaacc agaataatgg
     7021 cccgtatatc tggccccata ttgatgttga cacaaattca gccggtgaga tggtgattca
     7081 tgcagctgcc aatgaagcag tctgcgcaac cgcaccaacc gaagcgattg agacatcctc
     7141 attgctgtat taccgcaagg ttgggagcaa tccgtgggaa ggcccgatct ttatggattc
     7201 ggcctatttg atctcacagc tggttgtggc tgacaaaa
//
LOCUS       DSEB01000019            4777 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001460, whole genome shotgun sequence.
ACCESSION   DSEB01000019 DSEB01000000
VERSION     DSEB01000019.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001460"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(208..1056)
                     /locus_tag="ENO07_00325"
     CDS             complement(208..1056)
                     /locus_tag="ENO07_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HDS00439.1"
                     /translation="MKLDDKNQRWWDTAPAYILAYEDIELMNRDELRSVRLMLEYYKP
                     ELILEKNNVASIIVVFGGTRLLPRDQAEKNLQEAKARLEASPENKSFARQVRIAENLL
                     KKSHFYDEAREFASIVSRSAQSPVLREYVICTGGGPGVMEAANRGAHDVNAVSIGLNI
                     TLPLEQYPNPYITPEFCFRFHYFAIRKMHFMKRARALVIFPGGYGTLDEFFEAMTLIQ
                     TKVVKPIPVILFGRKFWDGIINFEALVEEGTIDPEDIEIFKYAETAEEAWELITDHYK
                     NNYKRP"
     gene            complement(1088..3658)
                     /locus_tag="ENO07_00330"
     CDS             complement(1088..3658)
                     /locus_tag="ENO07_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDS00440.1"
                     /translation="MVKALRSFRVTPKLPDELIRLREIANNIWWSWNDDAIRLFQRLD
                     RDLWDECHHNPVALLGEVSQDRMENLAKDDGYIAQLERVYQNLSGYLKEDTWFSRNHF
                     KESKPLIAYFSMEYGFTECLPIYSGGLGILAGDHIKSASELGLPLVGIGLLYQQGYFQ
                     QYLNKDGWQQEFYPNNDFYNLPIQLMQDDEGNALKISLDFPGRKVYAQIWEVKVGRIS
                     VFMLDSNISENNLHDRKITNQLYGGDKETRMQQELLLGIGGVRALEALGIKPIVYHMN
                     EGHSAFLALERIARGIEADNLDFHEAYLMVRQSNIFTTHTPVPAGIDEFLPDLIEKYF
                     SNYVPRLHLTFREFLELGRLEAPQQIAPFNMALLAIKCASFVNGVSELHGEVSRRMFQ
                     LAWKDLPAEEVPISHITNGAHTRSWISHEMATLYDRYLGPNWVNKPADQSVWKRVDQI
                     PDEELWQTHERRRERLIAFARRSLRKQLERRGALSSELDGALEALNPDVLTIGFARRF
                     AAYKRGTLILRDIERLKKILCNRARPMQIIFAGKAHPRDNEGKELIKQIVHVARDEQL
                     RRHIVFLENYDINLARYLVQGVDVWLNNPLRPMEASGTSGMKVVFNGGINISTLDGWW
                     VEGFSRETGWSIGMGEVYDNLEYQNEVEANALYDLLEKEVSRLFYDRGADGLPRKWIA
                     KMKDSMQGLCPRFNTNRMVKTYAEKFYLPAFRQSKILENRGMAELKDLSTWYRKIDRK
                     WNQIKVLELSNNVSTALAVGDEVEVQAVVRLGELKPVDVNVQLLIGRLDANGELVEPK
                     KYSMNSVEDLSSDTYKYSVRVSFKGSGNHGFTVRVLPDYRLLLNPFEPGLVTWANSNL
                     "
     gene            complement(3725..>4777)
                     /locus_tag="ENO07_00335"
     CDS             complement(3725..>4777)
                     /locus_tag="ENO07_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDS00441.1"
                     /translation="GQKLVLIGGSHYAGEIKKSVFTALNFILPQADVLSMHCSANIGK
                     KNDAALFFGLSGTGKTTLSTDPERRLIGDDEHGWSDDGVFNFEGGCYAKVIGLSEESE
                     PDIYETTRKFGTILENVALDFDTRRVDLDSDELTENTRASYPITHIESAVRDGTGGHP
                     ENIFFLTTDAFGIMPPIARLTPEQAMYHFISGYTSKIGGTEKGLGKEPKATFSACFGA
                     PFMVLHPYEYATLLAKKIAKHNAKIWLVNTGWTGGPYGVGERMKIKHSRAVLKAALNG
                     ELDSAEYHKDPVFGFEVPKECTGVPSEILDPRQTWDNPDDYDNKARELAERFKKNFEE
                     YKDEVPAEIEKEGPNI"
BASE COUNT         1157 a         1309 c         1071 g         1240 t
ORIGIN      
        1 actgaatctc accctcgaca attatcaaat caccaggtcc ggatcaggct cattctcatc
       61 acaggcggga accctggagc ggctcgaata tacaatttcg cagattggga atattgtcct
      121 caaaggcacc aatagcgggg acttcagcat aagaatgcgc accgattttc aaagcaggat
      181 aatctaccgg ctcgtcagat agttacttta cggtctctta taattattct tgtagtggtc
      241 ggttattaat tcccaggctt cctcggccgt ttcggcatat ttgaagattt caatatcctc
      301 ggggtctatc gtaccctctt caaccagggc ctcgaaattg attataccgt cccagaactt
      361 ccggccgaat aaaataaccg gtatcggctt gaccactttg gtctggatca atgtcatcgc
      421 ttcaaaaaac tcatccaggg taccgtatcc tccggggaag ataaccagtg cacgggcgcg
      481 tttcatgaaa tgcatcttgc ggatggcaaa ataatgaaat ctgaaacaga actcaggggt
      541 aatataggga tttgggtact gctccagcgg cagcgtaata ttgagtccga ttgaaacggc
      601 attgacatca tgcgcgccgc gattggccgc ctccattaca cccggccctc cgccggtaca
      661 aatgacatat tctctcaaaa caggcgattg ggcggaacgt gaaactatgg aggcgaactc
      721 gcgagcctca tcataaaaat gcgacttttt caggagattc tcggcaatcc ttacctgacg
      781 cgcaaatgat ttgttctccg ggctggcttc cagccgggct tttgcctcct ggagattttt
      841 ctcggcctgg tctctcggca gcagacgtgt tcctccgaaa acgacaatta ttgatgccac
      901 attgttcttt tcgaggatca gttccggttt gtaatattcg agcatcagtc ttaccgacct
      961 gagctcatcc ctgttcatca gctcgatatc ttcgtatgcc agtatgtagg cgggagcagt
     1021 gtcccaccag cgttgattct tgtcatccag tttcatctat tctccgcatc cagtaatgca
     1081 ttacaagtta gaggttacta ttggcccagg ttacaagacc cggctcgaat ggatttagca
     1141 gcagcctgta atccggcagg accctgacag taaaaccgtg gttgccgctg cccttgaagg
     1201 agaccctgac agaatattta tatgtatccg aggaaaggtc ctcgacagag ttcatggagt
     1261 acttcttggg ctctaccagc tccccgttgg cgtcgagcct gccgataagg agctgaacat
     1321 tcacatccac aggctttaat tcgcccagcc tgactacagc ctggacctcg acctcgtcac
     1381 ccacagcgag cgcagtgctg acattattgc ttagctccag caccttgatc tgattccact
     1441 tccggtcaat cttcctgtac cacgtcgaaa gatccttgag ctccgccata ccgcgattct
     1501 caaggatctt gctctgcctg aaagcgggta aatagaactt ctccgcatat gtttttacca
     1561 tacggttagt attgaagcgg gggcacaatc cctgcatgga gtccttcatc ttggcgatcc
     1621 atttacgggg cagaccatcg gcgccgcgat cataaaaaag cctggagact tccttctcca
     1681 gaagatcata aagagcattg gcctcaacct cattctggta ttccagattg tcatagacct
     1741 cccccatacc tatgctccat ccggtctcac gcgaaaaacc ttccacccac caaccgtcaa
     1801 gagtggagat attgatgcca ccgttaaata ccactttcat accgctcgtg cccgaggcct
     1861 ccatcggccg caggggatta ttgagccaca catccacacc ctgtaccaga tagcgggcta
     1921 aatttatatc ataattctcc aaaaagacaa tatgcctcct gagctgctcg tcacgggcga
     1981 catgcactat ctgctttatc aattccttgc cttcattatc cctcggatgg gccttgccgg
     2041 caaagattat ctgcatcggg cgggcacggt tacagagaat cttcttcagc ctttcaatat
     2101 ctctcagtat caaagtgccg cgtttgtagg ctgcaaacct gcgtgcaaag ccaatggtga
     2161 ggacgtcggg attaagagcc tccagagctc catccagctc ggaggacagg gcaccgcgcc
     2221 tttccagctg tttcctcagc gaccttcggg caaaggctat cagtctctcg cgccgccttt
     2281 catgggtctg ccagagttcc tcgtccggga tctggtcgac tcttttccag acagactgat
     2341 cggcaggttt attgacccag ttgggcccca aataacgatc gtacagcgtg gccatctcat
     2401 gcgatatcca cgaccgtgtg tgcgctccat tggtaatatg cgatatagga acctcctcgg
     2461 ccgggagatc cttccaggcc agctgaaaca tccggcgcga gacctcgccg tgcagctccg
     2521 agactccatt cacaaaagaa gcgcacttga tcgccagcag cgccatattg aagggcgcaa
     2581 tttgctgggg cgcctcgagc cggcctagct ccagaaattc cctgaaggtc aaatgcaaac
     2641 ggggcacata attggaaaaa tatttttcaa tcagatccgg aagaaattca tcaatgcccg
     2701 ccgggaccgg tgtgtgcgtg gtaaaaatat tcgactgcct gaccatcagg taagcttcat
     2761 ggaaatccag attatcggct tctatcccac gggcgattcg ctccagagcc aaaaaggcgc
     2821 tgtgaccttc attcatgtgg tagacgattg gctttatgcc gagagcctcc agtgctctca
     2881 cgcccccgat tcccagcagc agctcctgct gcatcctggt ttccttatct ccgccatata
     2941 actgattcgt gatcttgcga tcatgcagat tgttttcgga tatattgctg tccagcatga
     3001 agacagatat tctgcccact ttcacttccc atatctgggc atagaccttt ctgccgggaa
     3061 aatccaggct tattttcagc gcattaccct catcatcctg cataagttga atcggcaaat
     3121 tataaaaatc attattggga taaaactcct gctgccagcc atccttgttg agatactgct
     3181 gaaaatagcc ctgctgataa agcagtccga ttccgaccag gggcaaaccc agttcgctgg
     3241 ccgattttat atgatcgccc gccagtattc ccagaccccc cgaatatatc ggaagacact
     3301 ctgtaaatcc gtattccatt gaaaaatagg cgatcaacgg ttttgattct ttgaagtgat
     3361 ttctggaaaa ccaggtgtct tctttcaaat atccggacag attttgataa acacgttcaa
     3421 gctgggcaat atagccgtca tccttggcaa gattttccat cctgtcctgg ctgacctcac
     3481 ccagaagcgc tactgggtta tgatgacatt cgtcccataa atcccggtcc agtctttgaa
     3541 acaacctgat agcatcgtca ttccatgacc accagatatt gtttgctatt tctctgagcc
     3601 ttatcagttc atccggaagc tttggtgtaa ctctaaacga tcttaacgcc ttaaccatct
     3661 catctccatt tttactccta ttcaataaga acggcaaaac agctcaaaat ttgaacagaa
     3721 ttggctaaat atttggacct tctttttcga tctcggccgg cacctcgtcc ttgtattcct
     3781 caaaattctt cttgaaccgc tcagccagct ctctggcttt attgtcgtaa tcatcaggat
     3841 tatcccaggt ttgcctggga tctagaattt cggaggggac cccggtacat tctttcggca
     3901 cctcaaaacc gaaaaccggg tctttgtgat attctgccga atcgagctcg ccgttcaatg
     3961 ccgctttcag gacagcccgg ctgtgcttga tcttcatcct ctcgcccaca ccataaggcc
     4021 ctcctgtcca gcccgtattg acgagccaga ttttggcatt atgtttggcg atctttttcg
     4081 ccaggagggt tgcgtattca taaggatgca gaaccatgaa aggagccccg aagcaggctg
     4141 aaaatgtcgc tttgggttcc ttgcccaggc ccttttcagt ccccccgatc ttagaggtat
     4201 aaccggagat gaaatgatac attgcctgct caggcgtaag gcgcgcgatc ggcggcatga
     4261 tcccaaaagc atccgtggtt agaaagaata tattctctgg atgaccaccc gtaccgtcac
     4321 gcacggcgct ttcgatatgc gttatgggat atgaagcccg ggtattttca gtcaattcat
     4381 cgctgtccag atcgaccctt ctggtatcaa agtccagagc cacgttctcc agaatggtgc
     4441 cgaatttgcg agtggtctca taaatatccg gctctgattc ctcagacagc ccgataacct
     4501 tggcataaca gccgccttca aaattgaaga cgccatcatc gctccagcca tgctcatcat
     4561 ctccgatcaa acgcctttcc ggatcggtcg agagagttgt cttgccagtg cccgacaggc
     4621 caaagaacag ggctgcgtca ttttttttgc ctatatttgc ggagcaatgc attgagagca
     4681 catcggcctg gggaagaata aaattcaggg cggtgaagac tgatttcttg atctcaccgg
     4741 cataatgaga gccgccgatt aataccagct tctggcc
//
LOCUS       DSEB01000020            4768 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001463, whole genome shotgun sequence.
ACCESSION   DSEB01000020 DSEB01000000
VERSION     DSEB01000020.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4768)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001463"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            411..1355
                     /locus_tag="ENO07_00340"
     CDS             411..1355
                     /locus_tag="ENO07_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00442.1"
                     /translation="MFEGFSIKSSIYVFSIIIIIASLIAGCGSEDEPIQNMTEPLSSD
                     EFSTAYDSGQLDRIYYLTDEQAEKLSGFHGNYLDLINLQHLTVEQASSLAEFKGKGLD
                     LSRLDTISADLADELAKSKAYRLYLNGLKGISPAVAAELADFEGYELFLNGLTSLGAE
                     QAASLAGYDGKSLYLNGLKSVNDDEAQALSQSQAMYLTLDGLKSPGDRQVTVLSEFRG
                     QGLSMNGLEETTDAQAGHLASFGGTELFLNGLKHINAIQAEALADFGGDQLYLVGLES
                     LTEEQAKNLAGFEGELFVDNLDPQSMALYQKYRGPGTR"
     gene            1570..2511
                     /locus_tag="ENO07_00345"
     CDS             1570..2511
                     /locus_tag="ENO07_00345"
                     /inference="COORDINATES: protein motif:HMM:PF02665.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00443.1"
                     /translation="MSVIQILIYAAVLVFLVTNITRFIRMASTPIHLRWELYPVAHDV
                     KRFKYGGSFLEESDWYTKKLHKSMLGEIRVMLPEIFLLKGVWEYNRSLWLWSFTFHIG
                     LYLLTALIFVLILGGIIIGPNIFNVEAGYGSLSTIGQIIHSLSYAMLIAGCAIGTIGT
                     LGLIIKRSTDREMSSSSSFGSYFNLFFIAAIFISGLVFLFSGSAVDKLIKCYYSLITF
                     QPMPNLGAAFAVHAVISGLFILYLPFTHMTHFFMKYFTYHKVRWEDTPNIRGSKLEKK
                     IQEQLSYPVTWSAPHINADGKKNWADIATEEVKKDEK"
     gene            2501..4111
                     /locus_tag="ENO07_00350"
     CDS             2501..4111
                     /locus_tag="ENO07_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDS00444.1"
                     /translation="MKSRVKIEDISKPSKKLTHLDIDDLMPLPPPYDKIEEQPGWKAL
                     SEDALENLETSLDDTIAIGIPKPKTQEEEKELVKKFLSGLEKLLTRENNWTFLQPLLL
                     SLEHCAKCQTCTKACPIYEASGKQDIYRPTYRSEVWRRIVHKYLKPGGKFFGRLSHGD
                     IDLNWTTVARLMELSYRCTLCRRCAQTCPIGIDNGLITHELRKVFSQEMGIAPKELHE
                     KGSVLQLEVGSSTGMNAEVVKDNIEFIDEDMKDLTGIEVHTPWDKEGADVLLIHNAGE
                     ILAWPENPGAFAIVLEKAGISWTLSSDIAAYDGINYGLWYDDFQLGRVAIKHAEAAKK
                     LGVNRIVVAECGHAHKALTVIADRVLTGDFNIPRVSAMTLLEDIVFSGKIKFDPSKND
                     FPVTLHDPCNMVRLMGIVEPQRRILRYLCPQFREMEPHGVDNYCCGGGSGFAIMSGHN
                     FQDWRLAVSGRMKLKQVIEAFDDHPEPETKKYLCAPCSNCKGQLRDLFRYYGVQEKSG
                     IIYGGLVELIVNAMVDIKQPFINFDEMQ"
     gene            4138..4518
                     /locus_tag="ENO07_00355"
     CDS             4138..4518
                     /locus_tag="ENO07_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDS00445.1"
                     /translation="MSDGKLILVVDDEEDVVSYLTSLLEDNGYETISALDGAEGFKLA
                     REKKPALITLDITMPEESGVRMFRNLQQTEETSKIPVIIVTGVSSDFKQFIESRRQVH
                     PPEAYFEKPIDKDEFLKKVKELVS"
BASE COUNT         1265 a         1131 c         1185 g         1187 t
ORIGIN      
        1 aagcaggtag atcacgccgg catctttccc ggccggggtg atatcaaccg tcctcggccc
       61 aatgtgcctc atgtctgggg caaggcgatc gacttctgct ggttttatgt cagcgacggc
      121 agccgtcacg aggttgccgg cttcaggggc attcccatgg cgcaggccca ggcgggccat
      181 aactgcccgg actggggaat cctggaggat gttccgcgta cccccaagga gagattcctg
      241 cgcaatctcg ataccgaatt gaggcggcac tggacactgg ttttaagtcc ggatgatacc
      301 tcacgcggag tcggttcggg aaaccatacc acacatttcc atgttgatgt tcatgaatac
      361 cggagttatt gacggctctg gcaggtttca ataaaaagca gggaggcgaa atgtttgaag
      421 gcttttcaat taaatcgagc atctatgtct tttcaataat tataataata gcatctctca
      481 tcgccggctg tggttcagag gatgagccta tccaaaatat gactgagccg ttatcatcag
      541 atgaattcag tacagcctat gacagcggtc aactggacag aatttattac ctgaccgatg
      601 agcaggccga gaaactatct ggatttcatg gaaactacct tgatctgatc aacctccagc
      661 acctcacagt cgagcaggcc tccagcctgg cagaattcaa aggcaagggg ctggatcttt
      721 ccaggctgga taccatttct gcggacctgg ccgatgaact tgcaaaatcc aaagcatacc
      781 gtttatatct caatggttta aaaggtattt cgcctgccgt ggctgcagaa ctggcagatt
      841 tcgagggcta tgaattgttt ttgaacggtc ttacctcgct gggggcggag caggccgcat
      901 cactggcggg ctatgacggt aagtcacttt accttaatgg tttaaaaagt gtcaacgatg
      961 atgaggcgca ggccctgtcg cagtctcaag ccatgtacct cactctggat ggactaaaat
     1021 cccccggcga ccgtcaggtt acggtgctgt ctgaattcag gggacagggt ctgtccatga
     1081 atggccttga ggaaaccact gatgctcagg caggacattt ggcgtcgttt gggggaaccg
     1141 aactcttcct gaatggctta aagcacatta atgcaatcca ggccgaagca ttagctgatt
     1201 ttggcggtga tcagctctat ttggtgggac tcgaatccct cacagaggaa caggcaaaaa
     1261 atctggccgg attcgaaggc gagctttttg tcgataacct cgatccccaa tctatggccc
     1321 tgtatcaaaa atatagagga ccggggacca ggtaatctgc caagaacatc tcaaccggat
     1381 attgtgaaaa aaatatctaa atgcatccaa atcaacaaat attggtttga ctattcaggt
     1441 tattttctta tattcttcac ttaaagttta gcgaatccgt ttggattatt cgtatatttg
     1501 tagatggcag taatatatat actttagcac tctgataagg atttgagtca cagcttaagc
     1561 gagggtttca tgtcagtaat tcagattctg atttatgcag cagttttggt ttttcttgtg
     1621 acaaatatca caagattcat acgaatggca tccacaccaa tacatctccg ctgggaattg
     1681 tatccggtcg cccatgatgt caaacgcttc aaatacggtg ggtcatttct ggaagaatcc
     1741 gactggtaca ccaagaaatt acacaaatcg atgctgggag agatccgggt catgcttccg
     1801 gaaatatttc tcctgaaagg agtctgggag tataatcgca gcctctggct ctggtcgttc
     1861 acattccata tcgggctcta cctgcttact gcgctgatct tcgttttgat cctgggcggg
     1921 atcattattg gaccaaatat ttttaatgtc gaggcgggtt atggctcgct gtccacgatt
     1981 ggacagatca ttcacagtct cagctatgcc atgttaatcg ccggctgtgc gatcgggacg
     2041 ataggaactc tggggctgat tataaagcgc tccactgaca gggagatgtc ctccagttca
     2101 agtttcggat catattttaa tctatttttc atagccgcca tattcatctc tggcctcgtc
     2161 ttccttttct ctggcagcgc ggtggataaa ttgataaaat gctattacag cttgataacc
     2221 ttccagccga tgccgaatct gggcgctgct tttgcggtgc atgcagttat cagcgggctg
     2281 tttattctct atctaccctt cacccacatg acccactttt tcatgaaata tttcacttat
     2341 cacaaggttc gctgggaaga cactcccaac attcgcggca gcaagctcga aaagaaaata
     2401 caggaacagc tttcttaccc ggtgacatgg tcggctcccc atattaacgc cgacggtaag
     2461 aaaaattggg ccgacattgc taccgaggag gtcaagaaag atgaaaagta gagttaaaat
     2521 agaagacatc agcaagccgt caaagaaact cacccacctc gacattgacg atttaatgcc
     2581 cctgccccct ccctacgaca aaattgagga gcagcccgga tggaaggccc tttccgagga
     2641 tgcactcgaa aatctggaaa ccagtttgga cgatacgatt gcgataggta ttcccaagcc
     2701 aaaaacacaa gaagaagaaa aggaattggt aaagaaattc ctgagcggac tggagaagct
     2761 tctgaccagg gaaaacaact ggactttcct gcagcccctg cttctttctc tggagcattg
     2821 cgccaagtgc cagacctgca ccaaggcctg ccccatatat gaagccagcg gcaagcagga
     2881 tatctaccgg cccacctatc gttcggaggt ctggcgcagg attgtgcata aatatctcaa
     2941 gccgggcggg aaattcttcg gccgcctttc tcacggtgat atagatctga actggacaac
     3001 cgttgcccgc cttatggaac tttcctacag atgtacatta tgccgtcgct gtgcccaaac
     3061 ctgcccgatt ggaatcgaca acgggcttat cactcatgag ttgcgaaagg tattcagcca
     3121 ggagatgggc atcgccccca aggagcttca tgaaaagggt tcggttcttc agctggaagt
     3181 gggctcatct accggaatga acgccgaagt ggtcaaggat aatatcgaat ttatcgatga
     3241 agatatgaag gatctgaccg gaatcgaggt tcatacaccc tgggataaag aaggcgcaga
     3301 tgttctcctg atccacaatg ccggcgaaat cctggcctgg cccgaaaatc ccggggcctt
     3361 tgcgattgta ctggaaaaag ccggtatcag ctggaccctg tcatcggata tcgccgccta
     3421 tgacggcatc aattacgggc tctggtatga cgatttccag ctcggcaggg tggctataaa
     3481 gcatgccgag gcagccaaga agctgggtgt aaacagaata gtggtagctg aatgcgggca
     3541 tgctcataaa gctctgactg tgatcgccga cagggtcctg acaggtgatt tcaatatccc
     3601 ccgggtcagc gccatgaccc ttctggaaga tatcgtgttc tccggcaaga tcaagttcga
     3661 tccctccaag aatgattttc cggttactct tcatgacccc tgcaatatgg tcagattgat
     3721 gggcattgtt gaaccgcaga gaagaatctt gcgctacctc tgtccgcagt ttcgcgagat
     3781 ggagccgcat ggggtggaca attactgctg cggcggcgga tcggggttcg ccatcatgtc
     3841 cggccataac ttccaggact ggcgcctggc agtttcggga cgtatgaaac tgaaacaggt
     3901 gatcgaagcc tttgatgatc accccgaacc ggaaacaaaa aaatatctct gtgctccctg
     3961 ttcaaactgc aaggggcagc ttcgagatct gttccgatat tatggagttc aggaaaaatc
     4021 cggtataatc tacggcggcc tggtagaact gatcgtcaac gctatggttg acatcaagca
     4081 accgttcatt aactttgatg agatgcagta aaaaatatag cgggaggtta ataatatatg
     4141 tcagacggca aactgattct ggtagtggat gacgaggagg atgtggtatc ttatcttacc
     4201 tctcttctgg aggacaacgg atacgaaacc atctccgctc tcgacggcgc cgagggattc
     4261 aagctggcca gggagaaaaa gcccgccctg ataactttgg atattaccat gcccgaagaa
     4321 tcgggcgtca ggatgttcag aaatctgcag cagacagaag aaacctctaa aatcccggtg
     4381 ataattgtaa ccggcgtatc ctcggacttc aagcaattta tagaatcaag aagacaggtt
     4441 catccccccg aagcatattt tgaaaaaccg attgacaagg atgaatttct gaaaaaggtc
     4501 aaggagctgg tcagctaaca tgtagccacg gaaatttgaa tacaggcggg caaaattatt
     4561 attgtccgcc tttttattta taacagtaaa aagccgcccg gttttagccg gacggcttta
     4621 attgtatcat tcgaatgaga aagaattgcc ttttctcact ctgatgactt tttatttaga
     4681 acggacaaca gtctccgccg ggccatgatc caggtgagca attgttagga ggatcaccgc
     4741 cgacaaaggc gtagttgatg acccagac
//
LOCUS       DSEB01000021            8178 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000466, whole genome shotgun sequence.
ACCESSION   DSEB01000021 DSEB01000000
VERSION     DSEB01000021.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8178)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8178)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8178
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000466"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(247..320)
                     /locus_tag="ENO07_00360"
     tRNA            complement(247..320)
                     /locus_tag="ENO07_00360"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(284..286),aa:Arg,seq:tct)
     gene            complement(329..760)
                     /locus_tag="ENO07_00365"
     CDS             complement(329..760)
                     /locus_tag="ENO07_00365"
                     /inference="COORDINATES: protein motif:HMM:PF07681.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDS00446.1"
                     /translation="MKLRENDICFGFLILRLMVGIVFFMAGLFKLLDYMTIVGYFQSS
                     FAETWLPAFLVTIFAYVLPFAETILGILIFFGLLTRPALYLAGLLLVVLNFGLIVRGD
                     GGDAKSNIPYLIIIALDIILLNYNKYSLDHLLFGMSADFED"
     gene            complement(842..915)
                     /locus_tag="ENO07_00370"
     tRNA            complement(842..915)
                     /locus_tag="ENO07_00370"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(879..881),aa:Pro,seq:tgg)
     gene            1008..3443
                     /locus_tag="ENO07_00375"
     CDS             1008..3443
                     /locus_tag="ENO07_00375"
                     /inference="COORDINATES: protein motif:HMM:PF13432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDS00447.1"
                     /translation="MKLNRLLFVSAILIFLILAASLLKPGFLWGLKLPGYLDLYQSFL
                     IILSALSIYILISLAAGVPGMEQDTSDVKLKRIAILLFSAIFLVIIFYQFRSATMLLG
                     DGYLRANETNLGSKLHFTEPLDKFWQYIVYITLGDWFGLKSYQTYQIVSIFGGVLFFM
                     FGLWFADRYGKSRPEKILMAALIFSSAIMQIFFGYVESYSLSNPLFLFAIGATYVEVK
                     HSRSIIPGLLLYFTACLFHMSLLAYYPAFFILAIILWRAERSGNSLAEFIAAIAVPIL
                     SITMVIVLNNLQFGGSFDQNIFDYLIIPLLPNDTGYWLLSPTHLLDMLNQILLVAPAA
                     IIIFIAFNPFRRKFYTDHFSFFVALLSICGLLFLAFFDTAFGIARDWDLFSSIAIPLN
                     VLAGVMLINAHRASEDYSSFKKFSPVLPALIISFAFILLNSQEKPSVERYKDIINQSE
                     HGRHLNLENLGNYYSMEDDTVNYYDVLRKAGKYVEHPRYQHKIGMKLLEDGLTERAVE
                     HFYKALQIDPSYLPALNYLGMTYGLIGEKEPEAFTIAEKYFRLVQRYDPDYPHLHHNL
                     GGIYYQSRRYDQALSEYRMAIEADPDHLPSYEKLGMTFERTGQYDSSEYYYRYVIEHD
                     REGFDAYLHLGKMLYSSGKKETASQVLSSAEENFPDSEKQIEIAKTYAQINLYSDAVR
                     VLEELTQNPASPVDAYVTLANLYNLLNKQVQGISVLSRAAERNADPRSLIALAETFLQ
                     LGASDSAEVHLLNSISLDQTFYLGYRKLGTYYLLQGMQEKARQVLEMGLANVENPRDR
                     KSLSSLLDRINDQ"
     gene            3595..4674
                     /locus_tag="ENO07_00380"
     CDS             3595..4674
                     /locus_tag="ENO07_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00448.1"
                     /translation="MKSKLGFIAVSSVIVLSIIIIFAVSIERKDSGPNELQGRADSGR
                     VKDGNSNPGEVPEQVIPIEPEPAETLQISFAHVKDLTFWNDRIAAGTEGGVFTYKPED
                     SSYQFFYYANGLTEYDVNALLQIGDKLLVGTQGGLAQIDQAGRIEKIDFGFEPPVTAL
                     AESRGSILIGTAKDGLISYQDGSARQIFQVLDITSVVSSGDQIWVSTGGHGLFSFDGM
                     EWKKRFLVDDTTAFDYVADLDFKFQRLFAGTPYGLYVFDGGRWSLYNEEDGLMICDIR
                     HIDFKGWKIVVGTNDWGIYEIFEDWITPRDWTEGMEVTALACKDDYTVVGTPHEGIYI
                     AKGDQINHINPTPQTFTRPVVASIH"
     gene            complement(4825..5475)
                     /locus_tag="ENO07_00385"
     CDS             complement(4825..5475)
                     /locus_tag="ENO07_00385"
                     /inference="COORDINATES: protein motif:HMM:PF04893.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00449.1"
                     /translation="MDDALMAAYEVEEKSPGFPGMLKLLLFRPYTFFKKTVARDVFPY
                     LTFSAWLLGLAMALQLMDRTLLTPGGLVTGRISSFILLMLMSLVFMGLFYLIGGILFH
                     FYAYLADSRKDMKNSVAVTIYAWMPFVLAVFLTKLGLIIAFGSHYHSAPSENPILNIV
                     IYAVPGISILYGWFLAVFGAVVALGCKTLRAILMLFVVPILWFVFLYLLFQYLGFV"
     gene            5641..7767
                     /locus_tag="ENO07_00390"
     CDS             5641..7767
                     /locus_tag="ENO07_00390"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00144.22,HMM:PF11954.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDS00450.1"
                     /translation="MKRIYPALILVFLLISPCALQGQDDVDITGRWEGSIYLREMDLQ
                     IIIDFELDATGEYKGEVDIPQQGAEDLPVTNIRVDGRIVTSDLPNVPGDAKFAGVISG
                     DGDTLSGEFTQGGEVFKYLLIREDPEEAARRQALIQENLQKIRTFCDSMLAVWEPPGF
                     AVGVVYDGEVVFLEGFGYRNLEDSLPVTPRTLYAIGSTTKAFTTMAMGILADEDLLDW
                     NEKVINYLPDFRLKDEYATLHMTPIDLVTHISGMPRHDLLWYNSPFSREEIFNRMQYL
                     EFNEELRAAFQYNNLMFMTAGFLVGQLTRSTWEEFVEENILLPLEMLNTNFSVTVSWQ
                     SDDYALPYKYEGDSVVLTEFRNLDNVGPAGSINSSIEDMVNWLRLHLNKGEFNGRRLI
                     SEIELERMHTPHVIAGAMSPGQYKENSFAAYGLGWAVQYYRGNRRVWHNGGIDGFYTH
                     LNLYPDDDLGIITFVNLPGNRVIDVVSYYIADLFMGLEPVDWNTRLKGIDTGQDEEKE
                     DEEPEKEEPVKNTRPSHKLEEYAGQFENPGYGTFAVEYKDKELFASFNNMQSRLEHWH
                     YDVFRASDDVWEDTKINFLTNIKGDIDRISVPLEPAIDNIIFTKKPDAHLYDPNFLSM
                     LAGNYDIDGTICTVELRGENTLFVTVSGQPPYELEPYKGTEFNLKGLSGFSVEFELDD
                     EWEKVKGLRFIQPNGVFEAKRVESED"
     gene            7838..>8178
                     /locus_tag="ENO07_00395"
     CDS             7838..>8178
                     /locus_tag="ENO07_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008635738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDS00451.1"
                     /translation="MSDTDTKNRTSKLDNIKAEFPELNRVINSKPVVFLDSAATSQKP
                     MRVIDKLVEYYSNYNANVHRGVYSLSMESTEEYDTARELVREFINARSTNEIIYTRGT
                     TEAINLLARSWG"
BASE COUNT         2269 a         1954 c         1913 g         2042 t
ORIGIN      
        1 aaaccatctt cgctaatctt atctctgtaa ggggcatttt tcctctgaga catcagaatt
       61 acagaatagt tgggattcag gcgataattc atccccgctg aatagtctgg atattctcat
      121 tatcgcacat ttcccgatat gaaataatct ggtctttcat tgtgactaag ataataaatt
      181 cataagtcaa attcaaatta tgtatgtgag aatagggatg gctggacagt tctgtagatt
      241 aataaatacc cccggcagga ctcgaacctg ctaccctctg cttagaaggc agatgctctg
      301 tccggatgag ctacgggggc atcaaaatct aatcttcaaa atcggccgac atgccaaaga
      361 gcaagtggtc cagggagtat ttattataat ttaacaatat aatgtcaagt gcgattatta
      421 tcaaatacgg aatatttgac tttgcatcac caccatcgcc gcgaactatc agaccaaaat
      481 tcagaactac aagcaataga ccggcaagat acagggcggg acgggtcagg agcccgaaga
      541 aaatcaagat tccaaggatt gtctccgcaa atggcagaac ataggcgaaa atcgtcacca
      601 ggaaggccgg cagccatgtt tctgcgaagc tgctctgaaa atatccaaca atcgtcatgt
      661 aatccaggag tttgaacaac cccgccataa aaaagacaat tccgaccata agtctcagaa
      721 tcaagaaacc gaagcagatg tcgttttcgc gaagtttcat tcgaacctcc aaagatttag
      781 aaaatagtgt ttccgctatt taaagcccct tgcaatctga aatgttccct gtagaaagaa
      841 atcggggtga gaggatttga acctccgaca tcttgctccc aaagcaagcg cgctaccggg
      901 ctgcgccaca ccccgaatta gaaagaataa attcattttt caagcactgt caagcagtca
      961 tttcttttta gcttgaatta cggcggttcg cccatatatt ctcttgcatg aaactgaaca
     1021 ggctcttatt tgtttcggca attttgatat ttcttatatt ggcggcatcc ctcttgaagc
     1081 cgggattcct ctggggcctg aaactaccgg gctatctgga cctgtaccag tcattcctga
     1141 tcatattatc cgctctgtca atttatatat tgatctcgct ggcagccgga gtcccgggca
     1201 tggaacagga cacttccgat gtgaaattga aacgaattgc aatcctgctt ttttcagcca
     1261 tattcctggt cataatattc taccagttcc gttcggccac catgctcctc ggggatgggt
     1321 acctgcgtgc aaatgaaacc aatctgggat ccaaactgca cttcacagaa cccctggaca
     1381 aattttggca atatatcgtc tatatcacgc tgggtgactg gttcggcctc aaaagctatc
     1441 agacctacca gattgtatcg atcttcggag gtgtcctgtt cttcatgttc gggctctggt
     1501 ttgccgaccg ctatggtaaa tcgcggccgg agaaaatact gatggctgcg cttatcttct
     1561 catccgcgat tatgcagata ttttttggat atgtggaaag ctacagcctc tccaatccgc
     1621 tcttcctgtt cgcaatcgga gccacctatg tcgaagtaaa acattcccgc tccattatac
     1681 cgggcctgct gctatatttt accgcctgcc tcttccatat gtcgctgctg gcctattatc
     1741 cggccttttt tatactggcg ataatcttat ggcgtgctga gcgcagtgga aattcactgg
     1801 ctgaattcat cgctgcgatt gctgttccca ttctatcaat aaccatggta attgtactaa
     1861 ataatctgca atttggaggg agctttgatc aaaatatctt cgattacctg attatcccgc
     1921 tccttcccaa tgataccgga tactggctgc tgtccccgac gcatctgctc gacatgctga
     1981 atcagatact tttggtggca ccggcggcga taataatatt tatcgctttc aatcccttta
     2041 gaagaaaatt ttataccgat cacttcagct tttttgtggc cctgttatca atatgcggcc
     2101 tgctgtttct ggcgtttttc gacaccgctt tcggaatcgc ccgggactgg gatctgttca
     2161 gctcgattgc aattccctta aacgtcctgg caggagttat gttgatcaat gctcataggg
     2221 catctgaaga ttactcaagt ttcaagaaat tttcccccgt gctcccggca ttaattataa
     2281 gcttcgcatt cattctcctg aattctcagg agaaaccgtc agtggagaga tacaaagaca
     2341 taatcaatca gagcgagcat gggcgccatc tcaatctgga aaaccttggc aattattatt
     2401 ccatggagga tgataccgtc aactattatg acgtattaag aaaagcgggt aaatatgtag
     2461 aacatccccg ctatcaacat aagatcggaa tgaagctgct ggaggatggc ctgactgaac
     2521 gtgctgtgga acatttttac aaagctcttc agatagatcc atcctacttg cccgcgctga
     2581 attatctggg tatgacctat ggccttatcg gcgaaaaaga accggaagca ttcacgatag
     2641 ccgaaaaata tttccggctc gtgcaaagat atgatcctga ctacccgcat cttcaccaca
     2701 atctcggcgg tatctactac cagtccaggc gatatgatca agccctgtct gaatatcgca
     2761 tggcgatcga ggccgacccg gatcatttgc cctcatatga aaaactcggc atgacattcg
     2821 aaagaacggg acaatatgat tcctcggagt actattatcg ttacgtaatc gaacacgatc
     2881 gggagggctt tgacgcttat ctgcatctgg ggaaaatgct ctactccagc ggaaagaagg
     2941 agacagcatc gcaagtgcta tcaagtgcgg aagagaattt tcctgactcc gaaaagcaga
     3001 tcgagattgc taaaacctat gcccagatta atctttacag tgatgcagtc agagttctgg
     3061 aggaattgac ccaaaatccc gcatccccgg tcgatgccta tgttactctc gccaatctct
     3121 ataatctgct gaacaaacag gtccagggga tttccgtcct ctcacgagca gccgagagaa
     3181 atgccgaccc ccgctccttg atagcattgg cagaaacatt tcttcaactg ggggccagcg
     3241 attcggccga ggtacacctg ctcaattcca tctctcttga tcaaacattt tatctgggct
     3301 acaggaaact ggggacatat tatctgcttc agggaatgca ggagaaagcc cggcaggtgc
     3361 ttgaaatggg tctggcgaat gtcgaaaatc cccgggacag gaagtcctta agctctttgc
     3421 ttgatagaat aaatgatcag tagatgtgtc aatcctgaca cgccgcattt aatatccgca
     3481 tccctctttt taccctaata ctttatagta caatagatta caattccggc acggtttctg
     3541 ttttatatct gctgtgaaga tacaaaactg aaaccttcaa acaaggagtt cgagatgaaa
     3601 tcgaaattag gatttatcgc cgtctcatca gtcatagtcc tctcgataat cataattttt
     3661 gcagtgagta tcgagcggaa agattccggg ccaaatgaat tgcagggcag ggccgattcc
     3721 ggccgggtta aagatggaaa ttcaaacccg ggagaggtcc ccgagcaggt cattcccata
     3781 gagccggaac cggctgagac cctgcagata tcatttgcgc atgtcaagga cctgacattc
     3841 tggaacgata gaatcgctgc cggtaccgag ggcggtgtat tcacctacaa acctgaagat
     3901 tcttcttatc agtttttcta ctatgctaat ggtctgaccg agtatgacgt taacgccctt
     3961 ctccagatcg gtgacaaact cctggtcggc acccagggcg gtctggcgca aatcgatcag
     4021 gccggcagga tcgaaaaaat cgatttcggt tttgaaccgc cggttacagc actggcagaa
     4081 tcccggggca gcatcctgat cggaaccgcc aaggacggcc tgatttccta tcaggacggc
     4141 agcgcccggc agattttcca ggtgctcgac ataacctcgg ttgtttcttc aggagatcaa
     4201 atttgggtct ctacaggggg acacgggctt ttcagcttcg acggcatgga atggaaaaag
     4261 cgcttcctgg tggacgacac taccgctttc gattatgtgg ccgatctgga cttcaagttc
     4321 cagcgcctgt ttgccggtac gccctacggc ctgtatgtct ttgacggcgg cagatggagc
     4381 ctctacaatg aagaggacgg cctgatgatc tgtgatatca ggcatatcga cttcaagggc
     4441 tggaaaatcg tggtcggcac aaacgactgg ggcatctatg agatcttcga agactggatt
     4501 acgcccagag actggacaga gggtatggaa gtaacggctt tggcatgcaa ggacgactac
     4561 acggtcgtag gaactccgca tgaagggatc tatatcgcga aaggtgacca gatcaaccat
     4621 atcaatccta ccccacaaac ctttacgcga ccggttgtag cgtcaattca ctaacaacct
     4681 cctccaacgg aaaagccgct cttcccagag cggcttttct actctcgata agaaatggat
     4741 agggatttct tttcccgact ggttaaagcc caattagcaa gtcaagaatt attcaagaaa
     4801 aaagtatttt tatgataaat taatttacac aaatcctaaa tattggaaga gtaaataaag
     4861 aaacacgaac cacaatatcg gaaccacaaa gagcatcaag atcgcccgca gcgtcttgca
     4921 gccgagcgca acgaccgctc cgaaaaccgc caggaaccag ccgtacagga tactgattcc
     4981 aggaacggca tagatcacga tatttaatat cggattctcc gaaggagcgc tatggtagtg
     5041 acttccaaat gcaataatca gtcccagttt tgtcaggaat accgcaagca caaacggcat
     5101 ccaggcatag attgtgaccg caaccgagtt tttcatatcc tttctggaat ctgcgaggta
     5161 ggcatagaaa tggaataata tcccccctat caggtaaaac agtcccatga aaaccaggct
     5221 catcagcatc aacaggataa aagaggagat tcttcccgta accagcccac ccggggtaag
     5281 cagggttcta tccatcaact gcagggccat tgccagtccc aggagccagg ccgaaaaagt
     5341 caggtacgga aaaacatccc gggccacagt cttcttaaaa aaagtatagg gacggaaaag
     5401 gagcagtttt aacatgccgg gaaaaccggg gctcttctcc tcaacctcgt atgcagccat
     5461 cagagcatca tccatatttt atgcttatgg aaattattta caaatgttgc ataaaagttc
     5521 ataaaattaa ttttctccca cgggccaatt ttacagaaca ccatctgttt ttgcttgaat
     5581 tcaacttccg gatttgcgta atatataaaa atttcggtga aacttcaaag gagtgaaaaa
     5641 atgaaaagaa tatatcccgc attgattctc gtatttcttc tgattagtcc ctgcgcgctt
     5701 cagggccagg acgatgtgga tatcaccggg cgctgggagg ggagcatcta tctcagggag
     5761 atggatctgc agataataat tgattttgaa ctggatgcaa ctggtgaata caagggtgag
     5821 gtagatatcc ctcagcaggg agccgaggat ttgccggtaa ccaatatccg ggtggacggc
     5881 agaattgtaa cctccgatct ccccaatgtt ccgggagacg ccaaattcgc cggtgtaatt
     5941 tccggggatg gcgacactct aagcggtgaa ttcacccagg gcggagaggt attcaagtat
     6001 ctcctgatcc gtgaggatcc cgaggaggcc gcccgccggc aggcattgat acaggagaat
     6061 ctgcagaaaa taaggacttt ctgtgattct atgctggccg tatgggaacc gccaggattt
     6121 gcggtcggag tcgtttacga cggtgaggtt gtttttctgg aaggtttcgg ctacaggaat
     6181 ctggaggaca gcctgccagt aactccccgg acactatatg ccatcggctc aaccaccaaa
     6241 gcatttacca ccatggccat gggtatactg gccgatgagg atctcctcga ctggaatgaa
     6301 aaagtcatta attatctgcc cgacttcaga ttgaaggatg aatatgcaac tttgcatatg
     6361 accccgatag accttgtgac ccacatctcc ggcatgcccc ggcatgatct tctatggtac
     6421 aactcaccct tcagccggga agaaatcttc aatcgcatgc aatatctgga attcaatgag
     6481 gaattgcgcg cggcattcca gtacaacaac ctgatgttta tgacggccgg atttctggtc
     6541 ggccaactca cccgcagcac atgggaagag tttgttgagg agaatatcct tttaccactc
     6601 gaaatgctca atacaaattt ctcggttacc gtatcctggc agtcggatga ttatgctctg
     6661 ccctataaat acgagggcga ctcggtggtg ctgaccgaat tcagaaacct cgacaatgtc
     6721 ggcccggccg gctcgatcaa ttcatctatc gaggatatgg taaactggct caggcttcat
     6781 ctgaataagg gcgagttcaa tggaaggcgg cttatctctg agatcgaact ggaacgcatg
     6841 cataccccgc atgtgattgc gggtgcgatg tcacccggcc agtataaaga gaactcattt
     6901 gcggcgtatg gcctgggatg ggcggtgcag tactaccgcg gcaacaggcg ggtctggcat
     6961 aatggcggca ttgacggctt ttatacgcat ctgaatctct atcccgatga cgacctgggc
     7021 attatcacat tcgtcaatct ccccggcaac cgggtcatcg atgtggtcag ctactacatc
     7081 gccgacttat ttatggggct cgaacctgtg gactggaata cacgtctcaa ggggatcgac
     7141 accggccagg acgaagaaaa agaggatgag gaaccggaaa aggaagagcc tgtaaagaac
     7201 accaggccat cacataaact tgaggaatat gccggccagt tcgaaaaccc cggttatggc
     7261 acttttgcgg tagaatacaa ggataaagaa ctgtttgcca gtttcaataa catgcaatcc
     7321 cggctcgagc actggcatta tgatgttttc agggcaagtg atgatgtctg ggaagatacc
     7381 aaaataaact tcctgactaa tattaaaggc gatattgacc ggatttcagt accgctggag
     7441 cccgcaatcg acaatatcat attcaccaaa aaaccggatg cccatttata tgatccgaat
     7501 ttcctttcta tgctggcggg gaattatgac atcgacggca ctatctgcac ggtcgaatta
     7561 agaggcgaga ataccctttt tgttactgtg tcgggccagc cgccttatga gttggagcct
     7621 tacaagggca cggaattcaa cctgaaaggc ctgagcggat tcagcgtcga attcgagctt
     7681 gatgacgagt gggaaaaggt aaagggactg agattcattc agcccaacgg cgtttttgag
     7741 gcgaaaagag tagaaagcga agattaaggc ttcatttgtg gaacatgcgg cccagtgggc
     7801 atattatact gataagaaat cgaataggag atagaatatg tccgataccg atacaaaaaa
     7861 cagaacatca aaattagata acatcaaggc cgaatttccc gaattgaacc gggtaataaa
     7921 cagcaaaccg gttgtttttc tcgattccgc cgccacctca cagaaaccga tgcgggtcat
     7981 agataagctg gtagaatatt actccaatta taacgccaac gtgcaccggg gagtctattc
     8041 cctttcgatg gaatcgaccg aggaatacga cactgcccgc gagctggtgc gtgaattcat
     8101 caacgcacgt tcgaccaatg aaattatcta taccaggggc acgaccgagg caataaatct
     8161 gcttgctcgg tcgtgggg
//
LOCUS       DSEB01000022            1697 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1014549, whole genome shotgun sequence.
ACCESSION   DSEB01000022 DSEB01000000
VERSION     DSEB01000022.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1014549"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..358)
                     /locus_tag="ENO07_00400"
     CDS             complement(<1..358)
                     /locus_tag="ENO07_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00452.1"
                     /translation="MKFVRAKKLAAAALTFCILLIAAGPLMAQDQGEAKSLHVKHEIS
                     TTGLPLFGAIVVYNSQDIQGESLSRSTRSSKITMNDSVVSDVVVITITDMDAKKQIYV
                     NPKTNTYAEKEFQPSEF"
     gene            complement(546..1424)
                     /locus_tag="ENO07_00405"
     CDS             complement(546..1424)
                     /locus_tag="ENO07_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00453.1"
                     /translation="MRLSRLLIFIVLFVVLGCGGSDREDKNSGGFKADGSETVAAVIL
                     NYEISISDIPLVEPQTETIELAISTDALKYTSIAEIAVNGDSRKLERTSIIDFSEKSQ
                     TFLNREDSSFTVSFYDIAGEGDEGQREGDSQESGLIEISLSPTDEKRNIPPMHECRKV
                     LVRSQTRSQVSKKVSDSIPRLNGDLWVYRDDEILDIKTAYYDILSGLLTDPEFDGLGL
                     WRVLERLGIPREEIRGILGSLDGLAAEADLTVKMFSLGRKASVNISMRITGFEKKILP
                     AKYFDIPAEYNPAIGT"
     gene            complement(1449..1670)
                     /locus_tag="ENO07_00410"
     CDS             complement(1449..1670)
                     /locus_tag="ENO07_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00454.1"
                     /translation="MNLNQGWFETINQAIIGVPVEAEFKMTMPGKGETITYGMTMNLE
                     DYTTADIDESLLAVPAGYQEVSFSEFRMY"
BASE COUNT          452 a          434 c          348 g          463 t
ORIGIN      
        1 caaattcgga cggctgaaat tccttttccg cgtatgtatt agtcttggga ttgacataaa
       61 tctgcttttt agcatccata tcggtgattg taatcacaac cacgtcggag actaccgagt
      121 cattcatggt tatcttcgag gatcttgttg aacgcgaaag gctttctccc tgaatatcct
      181 gtgaattata gaccacaata gcgccgaaaa ggggcagacc ggtggtggaa atttcatgtt
      241 ttacatgcaa agatttggcc tctccctggt cctgcgccat cagcgggcct gcggcaatga
      301 gtaagatgca gaatgtcaat gctgctgcag ctaatttttt agctctaaca aacttcatgt
      361 gagaaactcc ctatgtttta atggacagtt acatatattg cttatcaatc tttaatctta
      421 aagttccggc aaaagtcagc tgaattttca taatccttta tacaatctac gtcgtgcgaa
      481 gattaaaatt cgattaaaat taattatgaa ggtttcatca gcataaatct gcataagatg
      541 gcctgctatg tcccgatagc gggattatat tcggccggga tatcgaaata tttggctggc
      601 aggatcttct tctcgaaacc ggtaattctc atgctgatat tcaccgaagc ttttctgccg
      661 agggaaaaca ttttgaccgt caaatccgcc tcggccgcca gaccgtccag agaacccaga
      721 attcccctga tttcctccct gggaattccc aggcgttcca gtacgcgcca caaccccaga
      781 ccgtcaaatt ccggatcagt cagcaaaccc gataaaatat cataataagc cgtcttaata
      841 tcgagaatct catcatccct gtagacccag agatccccgt ttaacctggg tattgaatcc
      901 gaaactttct ttgacacctg cgatctcgtt tgcgatctga caaggacctt ccgacattca
      961 tgcataggag gaatatttcg tttttcatcg gtcggggaaa gacttatctc aatcaatccg
     1021 gactcttggg aatctccttc gcgctggccc tcatcgccct ccccggcaat atcataaaaa
     1081 ctgaccgtaa agctggaatc ctcccgattc aaaaaggtct ggcttttctc agaaaagtca
     1141 attattgagg tacgttccag cttacgactg tccccattga ccgcaatctc ggcaatgctt
     1201 gtgtatttca gggcatcagt ggagattgcc agctcaatcg tctcggtttg aggttcaacc
     1261 agcggaatat cactgattga aatctcataa ttcagaatta ctgccgccac cgtttccgaa
     1321 ccgtcagcct tgaaacctcc gctattttta tcctcccggt cggaaccgcc gcagccaagg
     1381 accacgaaaa ggacaatgaa tattaacagc ctgctcaatc tcataacaaa ccgatcatct
     1441 tgccctgttt aatacatccg gaactcagag aagctgacct cctgatagcc ggcaggaaca
     1501 gccaagagcg actcatcaat atcggctgtt gtataatcct ccaaattcat tgtcattccg
     1561 taggttatgg tctcgccttt gccaggcatg gtcattttga attcggcctc gaccggaacg
     1621 ccgatgatgg cctgattaat ggtctcaaac cacccctggt tcaggttcac agaagccgca
     1681 atcccgaaga atgtggc
//
LOCUS       DSEB01000023            2023 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1009813, whole genome shotgun sequence.
ACCESSION   DSEB01000023 DSEB01000000
VERSION     DSEB01000023.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1009813"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1209)
                     /locus_tag="ENO07_00415"
     CDS             complement(<1..1209)
                     /locus_tag="ENO07_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013925339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDS00455.1"
                     /translation="MENYEKLGAFYLGREYDLEKKSLSEYPLLYDSKDLTTHAVCVGM
                     TGSGKTGLCIGLLEEAVIDGIPAVIIDPKGDLGNLMLAFSELKPEQFLPWVNADDART
                     KGVSVEDFAAAQAELWRKGLAAWGQDAERIRNYKNAAEFNIFTPGSTAGVPVSILKSF
                     AAPSKEILEDNDLFQEKVNTTVTSLLSLIGIDTDPLQSKDHILLSSILNQFWLQGRDL
                     SLEEFIRSIQEPPVERIGIFDLESFYPAGERFELAMRINNLIAAPGFSSWLEGVPLDI
                     GQILYTPEGRPRVSIFSIAHLNDKERMFFVSLLLTQMLGWVRTQPGTSSLRAILYIDE
                     IFGYMPPVANPPSKQPLLTLLKQARAFGLGVVLATQNPVDLDYKGLSNIGTWFIGRLQ
                     TERDKERMLDG"
     gene            complement(1336..1740)
                     /locus_tag="ENO07_00420"
     CDS             complement(1336..1740)
                     /locus_tag="ENO07_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015006685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HDS00456.1"
                     /translation="MAKLAEIEGIGKSLSSKFEEQGISTVESLLEKCCTTTGRKEIAE
                     KTGISEKRILRWVNMADLFRVKGIGEEYADLLEAAGVDTVPELAQRNAKNLAERMAAV
                     NQEKKLVRAVPPESRVSEWIEQAKVLPRKVEY"
BASE COUNT          497 a          592 c          431 g          503 t
ORIGIN      
        1 accatccagc atgcgttcct tgtcccgctc ggtctgcaaa cggcctatga accacgtccc
       61 gatgttggag aggcccttat agtccagatc cacgggattc tgggttgcca ggacaactcc
      121 cagaccaaat gcgcgcgcct gtttcaataa tgtcagcagg ggctgctttg agggcggatt
      181 tgctaccggc ggcatatatc cgaaaatctc atctatgtac aatatcgccc gcagactcga
      241 ggtccccggc tgagttctaa cccatcccag catctgggtc agaagcagtg agacaaagaa
      301 catccgctct ttgtcattca gatgagcgat ggaaaaaatc gaaacgcgcg gcctgccctc
      361 gggtgtatac aaaatctgcc cgatatccag cggaactccc tccagccagg atgagaatcc
      421 gggcgcggca atcaggttat taatcctcat ggccagttcg aagcgctccc cggccggata
      481 gaacgactcc agatcaaaga tcccgatacg ttccacaggc ggttcctgga tcgaccttat
      541 gaactcctcc agactcaggt cccgcccctg cagccagaac tgattcaata tgctggagag
      601 caggatatga tctttgctct gcagcggatc agtatcgata cctatcaggc ttaacaggct
      661 ggtgactgtc gtgttgactt tctcctgaaa cagatcattg tcttccagta tttccttcga
      721 gggcgccgca aaagatttca gaattgaaac cggaacaccg gcagtgctcc cgggcgtaaa
      781 gatattgaat tcagcggcat ttttataatt tctgatcctc tccgcatcct ggccccaagc
      841 ggccagaccc ttgcgccaca gctccgcttg cgccgcagca aaatcctcaa cagacacccc
      901 tttcgtgcgg gcatcatcag cattgaccca gggcagaaac tgctccggct tgagctcgga
      961 aaaagccagc atcagattgc ccaaatcccc cttcggatca attattaccg ccggaattcc
     1021 gtctattacg gcttcctcca gaagaccgat gcacaatccc gtcttgccgc tgccggtcat
     1081 gcccacgcag acggcatggg tagtcaagtc cttggaatca taaagtagcg gatactctga
     1141 aaggctcttc ttctccagat catattcacg acccagatag aaggccccca atttttcgta
     1201 attttccatc ccgatcaggc ccctggtata tacttacatc tgtaaaccat aaatgatgga
     1261 acccggcagg tccgggtccc acaaaaccgt ttattaataa actgatcaaa gatctaatag
     1321 cgtcagagtg tctgttcaat actcgacctt ccttggcagc accttggcct gttcgatcca
     1381 ttcactcacc cgcgactcag ggggaactgc cctgaccagc ttcttctctt gattcacagc
     1441 agccatcctc tccgcgagat tcttggcatt tctctgggcc agctcaggga cagtatccac
     1501 tccagccgct tccagcaaat cagcatactc ctcgccgatt ccctttacac gaaacagatc
     1561 ggccatgttg acccagcgca aaatacgctt ctcgctgatg ccggtctttt cagcaatttc
     1621 tttacgaccg gtggtcgtgc agcacttttc cagtagagat tctaccgtac ttattccctg
     1681 ttcttcaaac tttgaggaaa ggctcttacc tatgccctca atttcggcta atttcgccat
     1741 ttcgcactcc tttgtcaaaa tcaattaact actgagcagc actttcgata gctgcctata
     1801 ttcagcactg ggccagactc tccagtatct ttcccggcca gcaaatcaag gcaagtcagg
     1861 acaaatacat acgtagcaga accgattgac tgcctcacct tcagtccgaa agtctcacat
     1921 gacagtggtg atatttaact ggagatagcc gtactgaaaa accattattt tttcaaagtg
     1981 ctcacaattt aatttatttg ccactttaag tccagtgttt ttc
//
LOCUS       DSEB01000024            2231 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1007936, whole genome shotgun sequence.
ACCESSION   DSEB01000024 DSEB01000000
VERSION     DSEB01000024.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1007936"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..843)
                     /locus_tag="ENO07_00425"
     CDS             complement(<1..843)
                     /locus_tag="ENO07_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00457.1"
                     /translation="MRISKLIILFILTFVFTSPPCSAGELSFEVIPSSQHLTVGDTLT
                     LTMKVIVYGKPERYEVEPMSKLELPGFTTVSTSPRHRRGIEGGETFEERITTFRMVAE
                     EPGEYTIPSFEIPYHADSQSEDQYLSSQELSITVRPSPSGLARSKAPYIIGMFILVIL
                     ISVGGFYLWLQRTKSKQFEEEEDRNIAAKFKNWAEELNKLLASGKTDTFTQQAFNYVN
                     EFIEDNYRLGLKGKKFEKRMMIIHDAGVNLRLVELLKMTHDNLEEYKFGGMTHSTREL
                     SELLD"
     gene            complement(867..1742)
                     /locus_tag="ENO07_00430"
     CDS             complement(867..1742)
                     /locus_tag="ENO07_00430"
                     /inference="COORDINATES: protein motif:HMM:PF01040.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00458.1"
                     /translation="MISYLANVLRLMRWQNCLITMFVVVTAVFLLPFIPEVHEIIIAA
                     LAAAFITAYGNIHNDIADYRADKINHPERPLPSAELSPGTAILWLQMALAAGLILSLL
                     VNQRCFIVAAIASIALFFYSLFIKKIPLLANIWVGLIAALTFIYAGYISPLYTVWDFS
                     LINAGALIAFFFHLGRELIKDLQDMEGDLSSGLSTVALSWSPAAAKLLATMSFMAVGI
                     LAALLYLFLKPGLEFLVIFIIGVFLPLVILTVMMWRRSAQNAYRMISAWLKVLMPVGM
                     LTLLLAREKLPLDSF"
BASE COUNT          609 a          584 c          482 g          556 t
ORIGIN      
        1 atccagtaat tcactcaatt cccgtgtgct gtgtgtcata ccgccgaact tgtattcctc
       61 caggttgtca tgagtcattt tcagaagctc gaccagcctc agatttacac ccgcatcatg
      121 tattatcatc atgcgcttct cgaacttctt gcctttcagg ccaagacggt agttatcctc
      181 aatgaattca tttacataat tgaaggcttg ctgggtaaac gtatctgtct tgccggaggc
      241 cagcaatttg ttcaactcct ccgcccaatt cttgaatttg gccgcgatat tacgatcttc
      301 ctcttcctca aattgcttcg attttgttcg ttgcagccac aggtagaatc ccccaaccga
      361 gatcaggatt accagaatga acattccgat gatatatggg gcctttgatc tggcaagccc
      421 ggaaggtgag ggcctcacag ttattgagag ttcctgggat gagagatatt gatcttcgct
      481 ctggctgtcg gcgtgatacg gaatttcgaa agagggtatg gtatactctc ccggttcctc
      541 cgcaaccatc ctgaaagtgg ttattctctc ctcgaatgtc tctccccctt caattccgcg
      601 ccgatggcgg ggtgaggtcg aaactgttgt aaaaccgggc agttccagct tcgacatcgg
      661 ctccacttcg tagcgctcgg gcttaccgta gacaatcact ttcattgtaa gcgtgagggt
      721 gtcgcccacc gtcagatgct gactggaagg aataacttca aatgacagtt ctcccgcgga
      781 gcaaggcggt gatgtgaaaa caaatgtaag tataaataat ataatcagtt tgctaattct
      841 catatgcgtt ccaataaagc ctgccctcaa aaactgtcaa gcggtaattt ctctctggcc
      901 aaaagcaggg tcagcatacc cacaggcatc aagactttca gccaggcgga aatcattcta
      961 tacgcattct gagctgacct tctccacatc ataaccgtca aaattactaa cggcaaaaag
     1021 acgccgatta tgaatattac cagaaattcg agtccgggtt tcaaaaaaag gtagagtagc
     1081 gcagccagaa tcccaaccgc cataaatgac attgttgcaa gcagtttggc ggcagcgggg
     1141 gaccagctca aagccactgt ggataatccg ctgctcaggt cgccctccat gtcctgtaaa
     1201 tccttaatca gctcgcggcc caggtgaaaa aagaaagcta tcaacgctcc ggcattaatc
     1261 aggctgaaat cccagactgt atataatggg ctgatgtaac ccgcatatat aaatgtcagc
     1321 gcggcaatca atcccaccca gatatttgcc aggagcggaa tcttcttaat aaacaagctg
     1381 taaaaaaaga gcgcaatgga agcgatagca gccactataa aacatctctg attcaccagc
     1441 aaagacagta tcaggccggc tgccagggcc atctgcagcc acaatatagc cgtcccgggg
     1501 cttaattctg ccgaaggaag cggacgttcg ggatgattga tcttgtccgc tctataatcc
     1561 gcaatgtcgt tgtgaatatt gccgtatgcg gtgatgaaag cggccgccaa agcagcgatt
     1621 ataatttcat gaacctccgg tataaacggc agcagaaaaa ccgctgtcac aaccacaaac
     1681 atggttataa ggcagttttg ccacctcatc agtcttaata cattggccag atatgaaatc
     1741 aaaatctatt atccagtatt aaatacagtt ttccctcgct gaagctgtca ttcttactcc
     1801 agccgatatt taagtccagg ccgatctgcc cggaaaatag atgaaatccg aagcccgcgc
     1861 caaataaaaa ttcgtcctcg aacgccggag aatcctcttc agaataaata tccgtaagcc
     1921 gcgcatagcc cagatccgca aatatatagg ctttcagctg gcccggctga tatactccta
     1981 attccagatt agtccagccc gcccgcggag ttataaactg ttcctctgaa tatccgcgca
     2041 gcgtttccct gccgcccagc ttaatcatct ccgcgggcga gattaacctc tcgtcttcgg
     2101 aaaatcccgc gaaattccct tttgcatccg caaagaacag gttccaaatc cgtagaccgc
     2161 cccgcagcat caattctgct ttaaatggat tgaagctgtt atcatccggg acaatcccca
     2221 gagtacggta g
//
LOCUS       DSEB01000025            2078 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1009256, whole genome shotgun sequence.
ACCESSION   DSEB01000025 DSEB01000000
VERSION     DSEB01000025.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1009256"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            53..1063
                     /locus_tag="ENO07_00435"
     CDS             53..1063
                     /locus_tag="ENO07_00435"
                     /inference="COORDINATES: protein motif:HMM:PF07228.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00459.1"
                     /translation="MDIGDLEHFRTLLLEREHNLSELVNSKDHLLAKDADKVRELLVE
                     IKQSLDRIECGTYGACDCCDGQVEAFRLEIEPTSRICLDCLGDTEKAILEEELHVAGK
                     IYRSLLPEKLESIQGFKIATQSISAGTIGGDYYDFLPLPDGSGMRVVIADMVGKGLPA
                     GLLMSNLQGALRIFSEDIGKPADLIRRLNQWLCRNIPMSNFVTLACFCIESSDGEESQ
                     ITYANAGHWPPVLLRSDGKAEMLEPTGGILGVHKDFTFEQKDLSMSPGDLLFLYTDGV
                     TESRNDYGEMYEESRLLRFLQEHRDHSASSLLDKLLQEIWIFRGRSKFDDDLTVIALQ
                     KE"
BASE COUNT          566 a          485 c          511 g          516 t
ORIGIN      
        1 acctctaatt aaagctgtcg ttttaagaaa gagaaattga gaggtttttt ctatggatat
       61 cggagatctg gaacatttca ggaccctgct actcgagcgc gagcataacc tcagtgagct
      121 cgtaaactcg aaagatcatc tcctggccaa agatgccgac aaggtccgtg agctgctggt
      181 ggaaatcaag cagtctctcg atcgtattga atgcggcacc tatggcgcct gtgattgctg
      241 tgatggtcag gttgaggctt tcaggctcga gatcgaaccg acctccagaa tctgtctcga
      301 ctgcctggga gatacggaaa aggctattct ggaggaggag ctgcatgtag ccggaaaaat
      361 atatcgttca ctgctgccgg aaaaactgga atcgatccag gggttcaaaa ttgccaccca
      421 gtccatttct gcaggcacga tcggcggcga ttactatgat tttctgccct taccggacgg
      481 cagtgggatg cgggtcgtga tcgcagacat ggtcggaaaa ggccttccgg cgggattgct
      541 gatgtcaaat cttcagggcg cgctgagaat tttttcagag gatattggaa aaccggctga
      601 tctaatcagg cgtctgaatc aatggctgtg caggaatata cccatgagca acttcgtaac
      661 cctggcctgt ttctgcatag aatcctcgga tggagaggag tcgcagatca cctatgccaa
      721 tgcgggccat tggccgccgg ttcttctgcg atcggatgga aaagctgaaa tgctcgaacc
      781 caccggcggt attctcggag ttcataagga ctttacattc gagcaaaagg atctatcgat
      841 gtctcccggc gacctgctgt ttttgtatac tgatggtgtc actgaatcgc gaaacgatta
      901 cggcgagatg tacgaggagt cgcggctctt gagatttctt caggaacacc gcgaccattc
      961 agcaagcagc ctattggata agctcttgca ggaaatatgg atattccggg gaaggtcgaa
     1021 gtttgatgac gacctcacgg tcatcgctct gcagaaagaa taaaaaaaac ccgcccattt
     1081 ctgagcgggt cgagggatag agtatatatt tatttaatca acaatccgga ataccgtcac
     1141 catcggcgtc acatggatcg ttaccgccaa taaatacata gttgataatc cagactgcat
     1201 ctgaaatatt tacggcacca tcacagttta catttccgga ctcgagagga aatggcggat
     1261 ttccgccaat gaaaacgtaa ttggcgatat aaactgcatc gctgatattg accaaaccgt
     1321 cagaattggc gtccccgcat atcagctcgc caatgacacc gacttcggag gcaatatccc
     1381 gcatctccca gtcggagaca tcggcgctgt ctataatacc atccccggta tatgtaaaga
     1441 acaggccctg ataaactgcg ctgtccgttc cgctaaaacc gggatttaac aggagacggg
     1501 taactatccc gtcatagtta taagcgattc cttcagggcc gctgtaatcg ggtacaatct
     1561 cgccctcgaa gatgagatgt attgcactaa ggctgtccgg gaataccaga tgcacctcct
     1621 gagccagagt atcctgcaca aaaagtgcgg tgggttgaat gagctcattt ccatcatcac
     1681 ccagctttgg gtaaggactg atatcaccta taattacgcg atacatgaat atcaggtccc
     1741 ggacggttgg ataggtgctg tctttattgc agtctgtggc ccccagttgc atctgaaggt
     1801 ctatattgaa gatatccggt ccgtagatat aatagctttg aaacaataca taatcagcaa
     1861 tttcatatgg gatgccgtta agatttatat caccaatatc atcgatctca tcaatgcagg
     1921 cgacattaat tacgccattg taaaaatcaa ccagcttaat tttattaccg cccggaatat
     1981 tagcgcatga atccggcaaa ccgtggatcg tggggaaggt atcatcctgc tggatgaagt
     2041 caccaatata gggatcatag acactatccg atatatac
//
LOCUS       DSEB01000026           10367 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000282, whole genome shotgun sequence.
ACCESSION   DSEB01000026 DSEB01000000
VERSION     DSEB01000026.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10367)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10367)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10367
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000282"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..744
                     /gene="trpS"
                     /locus_tag="ENO07_00440"
     CDS             <1..744
                     /gene="trpS"
                     /locus_tag="ENO07_00440"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDS00460.1"
                     /translation="IFVQSQVKEHAELHLLLSMVVPTPWLERVPSYKEKSKSLGLDSY
                     GFLGYPLLQAADILLYRANFVPVGKDQLPHIELTREIARRFNGLYGEVFPEPQDKLTK
                     FAAVPGTDGNRMSKSYGNHICLSDTPEETANKISKMFTDPQKLRKGDPGRPEICPVFT
                     LHQIYSSKEEIEKIDRDCRSGALGCVADKKHLAENINKALAPIRARAAELRANPDTVW
                     DILRDGARKAGEKAGETMQMVREAMRLMQ"
     gene            810..1499
                     /locus_tag="ENO07_00445"
     CDS             810..1499
                     /locus_tag="ENO07_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002248207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00461.1"
                     /translation="MEKFEGPLDLLLYLIKKDEIDIYDIPVARITRQYLEYVEIMKML
                     NLDIAGEFILMAATLINIKSRMLLPKPEEDESEDPREALIMALLEYEKFKQASEVLRE
                     KESAEQQFLRRKDYSFLDLAPEETFTLEATIFDLMKAFKEALENLENDIYHTVRTEDV
                     NIEERVEVIERILSERGQATFQELYSDSPNRLVLIVTFLAILEMIKLNRLVLRQAIPF
                     GPIRIYLNKNE"
     gene            1492..2229
                     /gene="scpB"
                     /locus_tag="ENO07_00450"
     CDS             1492..2229
                     /gene="scpB"
                     /locus_tag="ENO07_00450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDS00462.1"
                     /translation="MNKDLTEELTEELTERMNLTEALLFASPEPLDHKKISRFLGRLS
                     KAQVNEIIQALNERYTEGGHSFRIREIADGYQLCLLPNMTMAVEKFLKRQRERRLTQA
                     SLETLAVIAYKQPVTKGEIEHIRGVAVDGPLNTLLERKLVTIAGRAEKVGHPLLYGTT
                     KKFLEYFGLKNLKELPRIEEFAEAYKVKKKSSQQELTLSEDEGGDMKEPKSRLKFVMK
                     APEEDEENKESLIEGEEAPMERNDEAE"
     gene            2183..2935
                     /locus_tag="ENO07_00455"
     CDS             2183..2935
                     /locus_tag="ENO07_00455"
                     /inference="COORDINATES: protein motif:HMM:PF01479.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDS00463.1"
                     /translation="MRVRRLPWNVMMRLNKFLSRCGVASRRQADEMILAGRVTVNGEK
                     ITKLGLVIDEINDVVRVDEETVRLPAEESYVVLNKPKGYISSLADRYNRPTVIDLIKG
                     VRDRVYPVGRLDLDTEGILLLTNDGELAYRLAHPKFDIKKEYVVTIKGLFPDHLTEKL
                     EQGVKLEDGYLARSKAKVLSANESNSVLQMELTEGKKREIKRIFAAMGYNVKSLKRIR
                     FADISVEGMRSGSWRYLNRKEVLKLRRKVGLH"
     gene            2965..3645
                     /locus_tag="ENO07_00460"
     CDS             2965..3645
                     /locus_tag="ENO07_00460"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDS00464.1"
                     /translation="MSSSTKIIAIDGPAGSGKSTTARMVAKILGFTFLDTGAMYRAVT
                     LFALEQKVPLLDAGALEQIASSIQIDFGENEDGQKVFADGRDITSDIRTAEVTDAVSE
                     VSAHAGVRRHMVAKQREMGDKYNIVAEGRDTTSVVFPHATLKIYLLASIEERARRRVI
                     DFQRMGKITTVQEQIKAIRARDEYDSRRQNSPLQQTRDSIAVDTTKLTIEDQVNKIIS
                     LFREKTRA"
     gene            3642..4259
                     /locus_tag="ENO07_00465"
     CDS             3642..4259
                     /locus_tag="ENO07_00465"
                     /inference="COORDINATES: protein motif:HMM:PF01553.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDS00465.1"
                     /translation="MKLFYRFSSAAARIYAKIFYGVRVYGQENLNFEGPALLASNHIS
                     LFDPPLLGGMIPFEIHFMAKAELFEKPLFGRLISALNAFPVKRGMIDRRALKISGEIL
                     ARGGRVLVFPEGTRQKSGILAKPRPGAAKLAIDYNVPVLPICLMNTNRLRHMIFSRNH
                     LKICYGELIDTGSFMPDASEKERIYGLSDKIMAEIANLQEFLKTA"
     gene            4491..6698
                     /locus_tag="ENO07_00470"
     CDS             4491..6698
                     /locus_tag="ENO07_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDS00466.1"
                     /translation="MAESAEKKKNTAKRTGGGKKASKKTSPKDKKQKSTTQKKRSKVE
                     ERASRLVTEVAGDVKTQETKRRQRVARKAEQKPARPVEEQIETVDSMKLTDLDDSDYD
                     SAEYEELMKMYEETLSDLREGEIINGKVLGVTRDDVIVDVGFKSEGIIAIEEFPEPVN
                     ISVGDEIEVYLDAFEDQNGQLVLSKRKADFLRVWDRIRESYESGELVPGRVSRRIKGG
                     LVVDLFGVDAFLPGSQVALRQVPNFDELVGREMELKIIKLNKNRRNIVVSRRVVLEQE
                     REELRSKLLAEIEAGQVREGVVKNITDFGVFIDLGGVDGLLHITDMSWGRISHPSEMV
                     SLGETINVKILDFDKETGRISLGLKQLSPYPWENIEEKYPVGKRVEGLVVSLTDYGAF
                     IELEKGIEGLIHISEMSWTQHIKHPSKIMQVGDKIEAVVLSVDKENEKISLGLKQLVE
                     DPWKTIDEKYPVGMKLKGRVRNLTTFGAFIELEEGIDGLIHISDMSWTKRIQHPSEIM
                     KKGDVVDVVVLKIDKENRRISLGHKQLFDDPWPNLSKNYAVGADVLGTVVRILDRGVI
                     VELPGGVEGFVPTNQLGKEDLAKPEEAFQIGDELPLQVIEFDQPGRRIVLSVDAYYKK
                     RERDELEQFLAKHPTKTVGMDEFAGEEVKEAEPEEAEKEAEPKPEEPEAGAEEEKKPA
                     ESPAPEAQAQDQVEKPAEQAPAAEEAQEQAEKPSEEPSESAEPEEGEEKPPSE"
     gene            6781..8064
                     /gene="mtaB"
                     /locus_tag="ENO07_00475"
     CDS             6781..8064
                     /gene="mtaB"
                     /locus_tag="ENO07_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HDS00467.1"
                     /translation="MRKVPKVSFYTVGCKLNQYETELIAQKMIDLGFERVDWAESADI
                     YIINSCSVTMKAAADSRRKLFAAKRMSPDAIVIMTGCYAQIQAQLLDKMPEVDLVVSN
                     DDKDKIISMISQIYPGINADLYENGSPLLRSLYKHSRALIKIQDGCNQKCSYCVIPLG
                     RGAERSRDYREIIEEIRLVSFNRYKEVILTGVHIGRYQFEGDNLTSLLSRILDETEIE
                     RIRLSSVEVNEFDDELIWLSARNKRICPHFHIPLQSGSDKILRSMNRPYKTDRYRAVT
                     ERLKSEVANSMVGADIIVGFPGEGEEDFGRTLEFVENSNIDYLHVFSYSDHPQARSFN
                     FDGKLASHIIKERNRILTGLGNRKWDKFLESQMKRDLPVLFEKRFARNSGMLTGLSDN
                     YIRVECDGGEDCFNTIQNVTPKEIIDGRIRGELRQ"
     gene            8750..9514
                     /locus_tag="ENO07_00480"
     CDS             8750..9514
                     /locus_tag="ENO07_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HDS00468.1"
                     /translation="MNAKEAIWEAYRKECAPEIREELLSQYLPLVKNVASRMAIGFPK
                     SVEVGDLVNTGVIGLIEAFRNFDPERGVKFETYAVPRIRGAILDELRSLDWVPRSTRA
                     RAREIERALVRLEGKLGRSPSDLELAGELNYELNDLYKALDDSNCTVLLSLDEMIYRE
                     DDNRQVPRVETVQAESKESVLNDLEKQELRAYLTIAISNLTEQEKLVIALYYYEELTL
                     KEIGEVMSISESRVSQIHTKAVLKLRGMVREKFALT"
     gene            9752..>10367
                     /locus_tag="ENO07_00485"
     CDS             9752..>10367
                     /locus_tag="ENO07_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00469.1"
                     /translation="MSSRPDNRTYDNGGQVKAVNPKPLSINFAAELPLSKINGSCEQN
                     SQTDPKALKSTGKTAANFRNSEAAESAVTAISKDNLKIMPAQAERADFFSEDNDTDMI
                     AEITGNIKLDQDTKAVSVETSDDRKDREQKSLKESITDDRAVKASGTEVSDRDVLTKN
                     GKSRIVLVRSSEDVKHQSGKPESADCNRQPARPWSLDVEIALCDL"
BASE COUNT         2922 a         2277 c         2796 g         2372 t
ORIGIN      
        1 atcttcgtgc agtcccaggt taaagaacat gccgaactgc atctactttt gtcaatggtg
       61 gtgccgactc cctggctgga aagggtgccg tcatataaag agaagtctaa aagcctgggt
      121 ttggactcct atggatttct gggatatcct ctattacagg cggcagatat tctactctat
      181 cgggctaatt ttgtgccggt cggcaaggac cagcttcccc atatcgaatt gactcgagaa
      241 attgcccgga ggtttaatgg cctttatggt gaggttttcc cggagcccca ggataagttg
      301 acaaaatttg cggcggtgcc gggtaccgat ggtaatcgaa tgagtaagtc ttatggcaat
      361 catatttgtc tgtcagatac acctgaggaa actgctaata agatttcgaa gatgtttact
      421 gatccccaga agctgagaaa gggtgacccg ggtcgtccgg agatctgccc ggtctttacc
      481 ctgcatcaaa tctattccag caaagaggaa atcgagaaga tagacaggga ttgccgttcc
      541 ggcgccctgg gatgtgtggc ggacaaaaaa cacctggccg aaaatataaa taaagccctg
      601 gcgccgattc gagcaagagc ggcagaactg cgggccaatc cggatacagt ctgggatatt
      661 ctcagggatg gcgcccgcaa ggcaggcgag aaagccggcg agactatgca gatggtgcgt
      721 gaagcgatga ggttgatgca gtgacggcag aagaactatt gaaaataaac ccgtattcgg
      781 gcgaattcga ggatacctac gaggtaaagc tggaaaaatt tgagggtccg ctggaccttc
      841 tgctctatct catcaagaaa gatgaaatag atatatacga catccctgtc gcccgcatca
      901 cccggcaata tctggaatat gtcgagatta tgaagatgct taatctcgat atcgccggtg
      961 agtttattct gatggccgcg accctgatca atattaaatc acgcatgctg ctgccgaaac
     1021 cggaagagga tgaatccgag gatccccgtg aagcgttgat catggccctc ctggaatacg
     1081 aaaaattcaa acaggcctcc gaagttttga gggagaagga atccgccgag cagcagttcc
     1141 tcaggcgtaa ggattattct tttctcgatc ttgccccgga ggagacattt accctggagg
     1201 ccacaatatt cgacctgatg aaggccttca aggaagctct tgaaaatctc gaaaatgata
     1261 tctaccatac ggtcaggaca gaagatgtta atatcgagga acgggtcgag gtgatcgaaa
     1321 ggatattgag cgagaggggc caggccacat ttcaggaact ttattccgac agccccaatc
     1381 gtttggttct gatagtaacc tttctggcca tcctggaaat gataaaactc aaccgtctgg
     1441 tcttaaggca ggccatacct tttggaccga taaggatcta cctgaataaa aatgaataaa
     1501 gatttgactg aagaattgac agaagaacta accgaacgca tgaacttgac cgaggccctg
     1561 ctgtttgcgt ccccggagcc gctggaccat aagaagatat cccgttttct gggaaggctc
     1621 agcaaagctc aggtgaatga gatcatacag gcgctgaatg aaagatatac cgagggcgga
     1681 cactcatttc ggatacgtga gatcgcagac ggatatcagc tctgccttct gcccaatatg
     1741 accatggcgg tggaaaaatt tctgaagcgc cagcgtgaac gcaggcttac ccaggcgagc
     1801 ctggagaccc tggcggttat cgcttacaaa cagcctgtta ccaagggtga gatagaacac
     1861 atccgcgggg ttgcggttga cggtcccttg aatacgcttt tggagaggaa gctggtcacg
     1921 attgccggcc gtgccgagaa agtcggccac ccgttattgt atggtaccac caagaaattt
     1981 cttgaatatt ttggattgaa aaatctgaaa gagctaccgc gcatagagga atttgccgaa
     2041 gcatataaag taaagaagaa atcctctcag caggaattga ctttgagcga ggatgagggg
     2101 ggcgatatga aagaacccaa atccagactg aaatttgtaa tgaaagcgcc ggaggaggat
     2161 gaggaaaata aggagtccct gattgagggt gaggaggctc ccatggaacg taatgatgag
     2221 gctgaataaa tttttatctc gctgcggcgt cgcctccaga aggcaggcgg atgagatgat
     2281 cctggcggga cgagttactg tcaatggcga aaagattacg aaactcggtc tggttattga
     2341 tgaaatcaat gatgtggtac gtgttgacga ggagaccgtc cgtctccccg ccgaagaatc
     2401 ctacgtagtg ctgaacaaac ccaagggcta catctcaagc ctggctgaca ggtacaaccg
     2461 tccaaccgta atcgatctca ttaaaggcgt cagagatcgc gtctatcccg tgggccgcct
     2521 cgatctcgat acagaaggta tactgctgct gaccaatgac ggcgaattgg cctaccgcct
     2581 ggcacatccc aagtttgaca tcaagaaaga atatgttgtc accatcaagg gcctgtttcc
     2641 ggaccatctt accgaaaagc ttgaacaagg tgtcaagctg gaggatggtt atctggccag
     2701 gtcgaaggcc aaggttcttt cggccaatga gtccaactca gtgctgcaga tggagcttac
     2761 tgagggtaag aaaagggaaa tcaagagaat ttttgctgcc atgggataca atgtaaaaag
     2821 tctgaagaga attcgttttg cagatatttc agttgaggga atgcgctcgg gaagctggcg
     2881 ctacctgaat cgtaaagagg tcttgaagtt aaggcgtaaa gtcgggttgc attaaatccc
     2941 ggaatacttt cactcttcaa aataatgtct tcttccacaa aaataatcgc tattgatggg
     3001 ccggccgggt caggtaaatc caccaccgca cggatggtgg cgaagatact gggctttacc
     3061 ttcctggata caggcgccat gtaccgggcg gtgactctgt ttgccctgga acaaaaagtg
     3121 ccgctcttgg atgcgggcgc gctcgagcaa attgcttcct ccatacagat tgattttggg
     3181 gaaaatgagg atggacagaa ggtctttgcc gatggccggg atatcacctc ggacattcga
     3241 acggccgagg ttaccgacgc tgtcagcgag gtttcggccc atgccggggt gaggcggcat
     3301 atggttgcca aacagagaga aatgggtgat aagtataata ttgttgcgga agggcgcgat
     3361 accacaagcg ttgtttttcc ccatgcaaca ttgaagatct acctgctggc ctcaatcgaa
     3421 gagagagcca ggcggagggt gattgacttt caacggatgg gaaagatcac cacagtgcag
     3481 gagcaaatca aggctatccg ggccagggat gaatatgatt ccaggcgcca gaattcaccc
     3541 ctccagcaga cccgggattc aatagctgtt gataccacca aactcaccat agaggatcag
     3601 gttaataaga taatcagtct ttttagagaa aagacccgcg catgaaactg ttctatagat
     3661 tttcttctgc agccgcccga atatacgcca aaatattcta cggcgtgagg gtctatgggc
     3721 aggaaaatct gaattttgag gggcctgccc ttttggcatc caatcacata tccttattcg
     3781 atccgccgct tttggggggc atgataccat ttgaaattca ctttatggcc aaggccgagc
     3841 tttttgagaa gccgcttttt ggaagactga tcagcgcact aaatgccttc ccggtgaaga
     3901 ggggtatgat tgaccgcagg gcattgaaga tttccggcga gatactggca cggggtggaa
     3961 gagttctggt atttccggag ggtacccggc agaaaagcgg catacttgca aaaccgcgtc
     4021 cgggggcggc caagctggcg atagattata atgtcccggt gctgccgatt tgcctgatga
     4081 ataccaatag attgagacat atgatctttt cccggaatca tttaaaaatc tgctacgggg
     4141 aacttatcga taccggcagc ttcatgcccg atgccagcga gaaagagcgt atttacggcc
     4201 tctctgataa gatcatggcc gaaattgcga atctgcagga atttttgaaa accgcatgat
     4261 agacgttcat atccaaatgt attcctcttt tattcgtaaa tcaggtgatt attcaatctt
     4321 atcttccgat tgtttcaggg cgggaataat cgggcattca ggacggttct ggaaaaccga
     4381 taatatcagg ttaatttcgc aaaatcaatt gacaaacctg aaattttgat tatatactcg
     4441 ctaacccttt caagggcaat gcttattttt aaaggaggaa ttaccgcaaa atggcagaaa
     4501 gtgctgaaaa aaagaaaaac actgctaaac gaacaggagg tggtaagaag gcttcaaaga
     4561 aaaccagtcc gaaggataaa aagcaaaagt caaccaccca gaaaaaacgc tcaaaggttg
     4621 aggagcgcgc atcacgtctg gtaaccgaag tagccgggga tgttaagacc caggaaacta
     4681 aacggcgaca gcgtgtagcc agaaaggctg aacagaagcc tgccaggcct gttgaggagc
     4741 aaatcgagac agtcgattcg atgaaactta ccgatctcga tgattccgat tacgattccg
     4801 ctgagtacga agaactcatg aagatgtatg aggagacgct ttcagatttg agagagggcg
     4861 agatcataaa cggtaaggta ttgggggtga cacgcgacga cgttatagtg gacgtcggct
     4921 tcaaatcgga gggtataatt gcaattgagg agtttccgga acctgtgaat atctcggtcg
     4981 gtgatgagat cgaggtatat ctggacgcat tcgaggatca gaacgggcaa ttggtgctct
     5041 ccaagcgcaa agccgatttc ttgagggttt gggatagaat ccgcgaatcc tatgaatccg
     5101 gcgaactggt tcccggccga gtcagccgca gaataaaggg tggtctggta gtggacctat
     5161 tcggggtgga tgccttcctt cccgggtcgc aggtcgcact ccggcaggtg cccaattttg
     5221 atgagctggt gggcagggag atggagctaa agatcatcaa actcaacaaa aaccgcagga
     5281 atatcgtggt ttcaaggcga gtggttctgg aacaggagcg cgaagagctc cgcagtaaac
     5341 tgctggcgga aatcgaggcc ggacaggtca gggaaggtgt ggtcaagaac atcaccgact
     5401 ttggcgtgtt catcgatctc ggtggagttg atggtctcct gcatattact gatatgtcat
     5461 ggggccgcat ttctcatccc tcggagatgg tctccctggg tgagactatc aatgttaaga
     5521 ttcttgattt cgataaggaa acgggccgta tatcgctggg cctgaaacag ctttcaccct
     5581 atccctggga aaatatcgaa gagaagtacc cggttggaaa acgggtggag ggcctggtcg
     5641 tttcactgac cgactacggc gcatttatcg aactggagaa aggcattgaa gggctcatac
     5701 atatatccga aatgtcctgg actcaacata tcaaacaccc ctcaaagata atgcaggtgg
     5761 gcgataagat tgaggcggta gttctttcag tggataaaga aaacgagaag atttcgcttg
     5821 gacttaagca gcttgtcgag gatccctgga agacaatcga cgagaaatat ccggtcggca
     5881 tgaagcttaa gggccgggtt agaaatctga cgaccttcgg cgcattcatc gagctcgaag
     5941 agggtatcga cggcctgatt catatttccg atatgtcctg gaccaagcgt atccagcatc
     6001 cctcggagat catgaagaag ggcgatgttg ttgatgttgt cgtgctcaaa attgataaag
     6061 aaaaccgcag aatctctctc ggccataagc agctattcga cgatccctgg ccgaatcttt
     6121 ccaagaatta cgccgttggg gctgatgtgc tcggcactgt tgtgcgcatt ctcgaccgcg
     6181 gtgtgatagt ggagctgccg ggtggtgtgg agggtttcgt tccgaccaac caactgggca
     6241 aggaagatct tgccaagccc gaggaggcct tccagatcgg cgatgaactg ccgctgcagg
     6301 taatcgagtt cgatcagccc ggacgcagga ttgtgctctc ggtcgacgct tattacaaaa
     6361 agcgtgagag agatgagctg gagcagttcc tggccaagca tcctaccaag actgtgggta
     6421 tggatgagtt cgcaggtgag gaagtaaaag aggccgaacc ggaagaggct gaaaaggaag
     6481 cggagccgaa acctgaagag ccggaagccg gggctgaaga ggagaagaag cccgcggaat
     6541 ctcccgcacc tgaagcgcag gcgcaggacc aagtggaaaa gcctgctgag caggctcccg
     6601 ctgctgagga ggctcaggag caagcggaga agccttcgga ggagccttcc gaatctgcgg
     6661 aacctgagga gggtgaggaa aaaccacctt cagaataaat tgttcctttt aaatcaaacg
     6721 ggcggtaata tccgcccgtt ttttttctga aaaaattgcg ttttaactta aattgaggtc
     6781 atgcggaaag tgccgaaagt atccttttat acagtcgggt gtaagctcaa ccagtatgaa
     6841 actgaattga tcgcgcagaa gatgatcgac ctcggtttcg aacgggtcga ctgggccgag
     6901 agtgcagata tttatataat caacagctgc agcgtgacca tgaaagcggc cgccgattcc
     6961 cgccgcaagt tgtttgccgc aaagagaatg tcccctgatg caatagtcat aatgaccggc
     7021 tgttatgccc agattcaggc gcagctcctg gataagatgc ccgaggtcga tctggttgtg
     7081 tcaaatgatg ataaagataa aatcatctca atgatatcgc agatctatcc aggtatcaat
     7141 gcggacctgt atgagaatgg cagccccctg ctgagatcac tgtacaaaca cagccgtgct
     7201 ttgataaaaa tacaggacgg ctgcaaccag aaatgcagct attgtgtaat ccccctggga
     7261 cgcggagcgg agagatcccg cgattacagg gaaatcattg aagaaatcag gctggtatca
     7321 ttcaatcggt ataaagaggt gatcttaacc ggggtgcata tcggccgcta tcagtttgaa
     7381 ggggacaatc taacttcatt gctgagtcga attcttgatg aaactgagat tgagcgcatt
     7441 cgtctttcct cggtagaggt gaatgaattt gatgatgaac tgatctggct ttccgcccgg
     7501 aacaagcgga tttgcccgca ttttcacata cctctgcaga gcggctcgga caaaattctg
     7561 cgctcaatga accggccata taagactgat cgttacagag ctgtaaccga aagattgaaa
     7621 tcagaagttg cgaactcgat ggtcggtgct gatatcattg tcggatttcc gggtgaaggg
     7681 gaggaggatt ttgggagaac cctggaattt gtagaaaata gtaatatcga ttacctgcat
     7741 gtcttcagct actctgacca tccccaggcc agaagtttca atttcgatgg aaagcttgcc
     7801 agtcatatta ttaaagagcg caaccgaatc ctcaccggcc ttggaaaccg caagtgggat
     7861 aaattcctgg aatcgcagat gaaacgggat ttgccggtat tattcgaaaa gcggtttgcc
     7921 aggaattcgg gaatgctcac cggattatcc gacaactata tccgggttga atgcgatggc
     7981 ggagaagact gctttaatac gattcagaat gtcacaccaa aagagataat cgatggacgt
     8041 atcaggggcg agttaaggca atgacccctt gagcgtcaat cccattttca aatccccctt
     8101 ctatttagcg cggaaaatat tatcgagctt ttaaaaaggc ggggctgaag aaattctttc
     8161 ccggctgata gaaaatattt ccgcaaaaaa ctcttaccgt ttttccagca gtaactataa
     8221 accattggca gacagccgaa taacctccac cgtcgaagac ggcatccgac ttgctaatct
     8281 atctctcagt taaacttata gcctgaaata tgaatatatt aaattttgca catttaagat
     8341 tagaagtgat cctcttgact ctcctgggag gttccgggct gtttctggtt gaacctcttt
     8401 caggtccgaa cttgatcccg gcgaatctga aagtaatgcc agtgtccaca ccggcgactg
     8461 cagcgtgtga gcgggatatt aaattcatct tcagccgcca taatatactg aacagcgaaa
     8521 gggacgattg tccattcttt gaacattcgc aaggagtggt gcaatatatt ttctgccagc
     8581 ccgtaaaaca tcaggttgag ggcaacgaca cctgcagggg taagataaat tctgattttg
     8641 tgttaagttc cccgtcatcg gttaccgata gaattacagg tattctgcat gcggggggac
     8701 ttgagaataa catcgtaaac aaatgtcaaa gctcaaggga agactcttta tgaatgctaa
     8761 agaagcgatc tgggaggcgt accgcaagga atgcgcgccc gaaatcagag aagagctgct
     8821 gtcgcagtat ttgccgctgg taaagaatgt ggccagccgg atggcgatcg ggtttccgaa
     8881 atcagtcgaa gtcggtgacc tggttaacac aggtgtgatc ggtttgatcg aggccttcag
     8941 gaacttcgac cccgaaagag gcgtcaagtt cgagacctat gctgttccca gaatccgagg
     9001 agccatcctc gatgagttac ggtcgctgga ttgggttcca cggtcgacac gtgccagggc
     9061 cagggagata gaacgggccc ttgtcaggtt agagggtaaa ttgggacgct ctcccagcga
     9121 cttggagctg gccggagaac tcaattacga gctcaacgat ctctacaagg ccctggatga
     9181 ttccaactgc accgtgctgt tgtcgctgga tgagatgatc taccgcgagg atgacaaccg
     9241 ccaggtaccc agggtggaaa cagtgcaggc cgagtccaag gaatcggtat tgaacgacct
     9301 cgagaaacag gaattgcggg catacctgac aatcgcaatt tcaaacctga cagaacagga
     9361 gaagcttgta atcgctcttt attactatga agaattaacc ttgaaggaaa tcggcgaagt
     9421 catgtcgatt tctgagtcgc gagtctcaca gatccacacc aaggcagtgc tcaaacttcg
     9481 agggatggtg agagaaaaat ttgcgctgac ttgagcacat ggaatgagtg cttgtccctg
     9541 caagcaagtc aacgcactcg ccggtaagag cctttgaggc gggaagataa cacaggtaaa
     9601 gaaatccgtt ccggtaagga agaaaaaagc gcctgtgagc attaataagg aacatatagg
     9661 aaatatcagg ataaacgacg ccggcgggcg gaagatccgc aggatgggat aaactgccgg
     9721 ctctcgcaat actatgggaa aggatgggaa gatgtccagc cgacccgaca atcggactta
     9781 tgataatggc gggcaggtaa aagctgtaaa cccaaagcca ttatctataa atttcgcggc
     9841 cgagctccct ctgagcaaaa tcaatggttc ctgtgagcaa aatagtcaga ccgatccaaa
     9901 agcattaaaa tccacaggca agacagccgc aaatttccgg aattcagaag ccgcagagtc
     9961 cgctgtcaca gcgatttcaa aagataatct aaagattatg ccggcgcagg cagagagggc
    10021 ggatttcttt tcagaagata atgacaccga tatgattgct gaaataaccg gaaatattaa
    10081 acttgatcag gatacaaagg ctgtatcggt ggaaacaagt gatgatcgca aagaccgtga
    10141 acagaagtca ttgaaagaat cgattactga tgacagagcg gttaaggcgt ctggcactga
    10201 agtgtctgat agagatgtgt tgacgaaaaa tggtaaatcc cggatcgtgc ttgtgcgctc
    10261 ctctgaggat gtcaagcatc aatctggaaa gccggaatca gcagattgca atagacaacc
    10321 agcccgtccc tggtccctcg atgttgaaat tgcactgtgc gatttac
//
LOCUS       DSEB01000027            4486 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001684, whole genome shotgun sequence.
ACCESSION   DSEB01000027 DSEB01000000
VERSION     DSEB01000027.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001684"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            325..528
                     /locus_tag="ENO07_00490"
     CDS             325..528
                     /locus_tag="ENO07_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00470.1"
                     /translation="MGKVIEIEYSELKNLGDYQHRRIGARAAVGEDEEPEDAMKHLRN
                     FVWKEFEKARCTFSYDEDAGQPI"
     gene            525..932
                     /locus_tag="ENO07_00495"
     CDS             525..932
                     /locus_tag="ENO07_00495"
                     /inference="COORDINATES: protein motif:HMM:PF05866.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDS00471.1"
                     /translation="MTELLTFKIEGWPRPQQRPRKFKSTWASNYHPGAAAWKEYCRRY
                     AQNTWKKAPLPAGIFLRVAFMFYFPRPRKPKWRFPCTPTDGDLDNLIKATADALNGLL
                     WKDDRFIVADGGSFKTYCLPGQEPGALIRVEEI"
     gene            complement(1254..1745)
                     /locus_tag="ENO07_00500"
     CDS             complement(1254..1745)
                     /locus_tag="ENO07_00500"
                     /inference="COORDINATES: protein motif:HMM:PF10097.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HDS00472.1"
                     /translation="MNMGKRKRKSKKTKRAQKQNNGQIQSYGPDSPNISPPLIGTAGF
                     RAESFSGPIPHPKILEQYDNIVPGSADRIIEMAETQAAHRQDLERRVVKSDIFNSRLG
                     LIFGFVIALCFICGSAYLLYNDKTTEGFLMGGVTLFSLVGVFVYGSQQRRKERETKAS
                     MEP"
     gene            complement(1684..1938)
                     /locus_tag="ENO07_00505"
     CDS             complement(1684..1938)
                     /locus_tag="ENO07_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015163968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00473.1"
                     /translation="MNDIRNSLTFRLFALPSFTEGMARIFDFNGFLQVYNVSRTPEDA
                     DFEAISNDWRVTGWDIKQAMDEYGQKEKEEQEDKESAKTK"
     gene            2335..2832
                     /locus_tag="ENO07_00510"
     CDS             2335..2832
                     /locus_tag="ENO07_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDS00474.1"
                     /translation="MPNGKWESHVKDRIDTIRAWKRSGLTDKEIAENLGIGYTTFRRY
                     MKDHRTLWSALKTGKSDADALVENALFKRAMGYRYEEVEESVTKNKEGEITGTRTKRI
                     KKQIAPDVTAIIFYLKNRKSQDWHDRHALEHSGPDGTPLNPPVIHAYMPPNGRELLPE
                     ESDVR"
     gene            2829..4253
                     /locus_tag="ENO07_00515"
     CDS             2829..4253
                     /locus_tag="ENO07_00515"
                     /inference="COORDINATES: protein motif:HMM:PF03237.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDS00475.1"
                     /translation="MKKCLILTAIFMILGAAMADQREVIRYSPQPGPQEEFLKTPADI
                     AIYGGAAGGGKTWALLYEALRWVEVSNYGAVIFRREYTQIRAEGGLWDESLSMYPHWR
                     GVAREGYLDWRFPARSKIRFAHMQHENDKHNWDGSQITLIGFDQAESFSWSQIAYMFS
                     RNRSTCRVSPYMRMTCNPDPDCWLREFLRWWIDDDTGFAIEKRSGKIRYMVNVSNEIH
                     WGDSPEELKKKFGSDCDPKSCTFIPSSVYDNKILLKRNPGYIANLQALQKVDRERLLR
                     GNWNIRETAGNFFQRPWFEIVNAAPALEEEIRYWDRAATETKPGQEKKASWTAGVRIG
                     RDSRGIFYVTDICRFQGSPMKVEETIKNVASQDGHKVKVGIEQDPGQAGKAEALGYAR
                     LLAGYSVVLNPVREAKGIRARPLSAQAEAGNIKLVRAGWNDAYLNELENFDGSEGCVS
                     DQTDASSGAFHLLTNIKQAGVWGR"
     gene            4266..>4486
                     /locus_tag="ENO07_00520"
     CDS             4266..>4486
                     /locus_tag="ENO07_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00476.1"
                     /translation="MQMEHVGFLQLLISFLSGLVGVGVGWGALRNQVKNNTRDIVEIY
                     SRLASIHGQNSGRSIFVPREECMERHEDMT"
BASE COUNT         1195 a         1050 c         1191 g         1050 t
ORIGIN      
        1 gacaatgtat gtcccctaac accgccctaa agatcgatcg gccaatggcg gccgctttct
       61 ggctcgagaa aaggtatttg cggttgtctt gttgcccccc cctcgcgagg gcgtggattg
      121 aaacgaaccc tgaatcagcc agagagggag agatctggcc agatcataat gcaaagtctg
      181 aaggaggtga tgccatggtc tgaaaaaggc atattttctg aatccatatt tgttttatta
      241 gcacgtcgga tgtgagtgca aaaacaacca aaaaacgtcc aagggcggtc ggtataaacg
      301 ccggctgccc gatgaaagga tgagatgggg aaggtaattg agatcgaata ttcggaactg
      361 aaaaatttag gagactacca acatcgaaga attggagctc gggcggccgt cggcgaagat
      421 gaggagccgg aggacgccat gaagcattta agaaactttg tctggaaaga atttgagaaa
      481 gcccggtgca cgttcagtta cgatgaagat gcgggtcagc cgatttgacg gaactcttga
      541 ctttcaaaat agaaggctgg ccccgaccgc agcagaggcc ccgcaaattc aagtcaacat
      601 gggcttcgaa ttatcacccc ggggccgcgg cctggaaaga atattgccgg cggtacgcac
      661 aaaatacctg gaaaaaggca ccgctaccgg caggaatatt tctacgcgtg gccttcatgt
      721 tttatttccc gaggccaaga aaaccgaagt ggcggtttcc atgcacaccg accgacggcg
      781 atctggataa cctgatcaag gccactgctg atgcattgaa tgggcttctg tggaaagacg
      841 accgttttat cgttgccgat ggaggatcgt tcaagaccta ttgtcttcct gggcaggaac
      901 cgggggcgtt gataagggta gaggaaatct gaaatgccaa gtagaatgaa gctgggtaag
      961 gcaaagttaa aaaaccgttt attggataat ccaaacaata agaggtatgt tcatggcagg
     1021 caagccgagc aaggaaattc aactcagaaa tgacaaaatt gtggccgaat ttgagggaaa
     1081 gatcagaaaa aatccggacc agccggtgag aaagattgcc cgagacctcg ccaagaaata
     1141 cgacttgagt gagaggcgaa tactgggtat tatttatgac tgctgatagg cccatccgaa
     1201 aagagaagcg ctcggcagat ttcacctggt tctttataaa tatttctctt atcttatggt
     1261 tccatggatg ccttggtttc tctctctttg cgccgttgct gagatccata aacaaatact
     1321 ccaacgagcg agaagagcgt cactcctccc ataagaaacc cctcggtagt cttatcattg
     1381 taaagtagat aggctgaacc acaaatgaag catagagcga taacaaatcc gaagatcagt
     1441 cccaaacggg aattgaaaat atcagattta actactctgc gctcaaggtc ttggcgatgg
     1501 gcagcttggg tctcagccat ttctattatt cgatctgcag agccgggaac tatgttatca
     1561 tattgttcca ggatctttgg atgtggtatt gggccgctga aactttccgc tctaaatccg
     1621 gcagtaccta taaggggggg agaaatattt ggggagtctg gcccatagct ctgaatttgg
     1681 ccattatttt gtttttgcgc tctctttgtc ttcttgctct tccttttcct tttgcccata
     1741 ttcatccatt gcctgcttta tatcccaccc tgtcactcgc caatcattcg aaatggcttc
     1801 gaaatctgca tcttcgggag tcctgcttac attataaacc tgcaagaatc cattaaaatc
     1861 gaaaattctt gccattccct ctgtgaaaga cggcaaagca aataatcgaa atgtcagact
     1921 gttccttata tcgttcatcg tccccaatcc cgtctgtagg tttatatcgg ctcttggctc
     1981 atgaatgtca agaacttttc cgttagttca tttagattcc ttccgattat ttaaacacat
     2041 ttttccttaa attgtttcgg ttgacagcaa gttgttatca tccatgcaat tacggatact
     2101 tggcgttgca ttcttgtttc aatatttgat tcttctgcga tgtcattaat gacaacaaat
     2161 gatgtcatca atggtaacgg gtcattgtaa ctctccaaac aatagagctt cttacctgcg
     2221 catatcatag gttcctcctt gtgaaaaggt tgccggtgtg tgggaaaaac ttaaaacccg
     2281 cacaccggat taagattcag aggaagatga tggcggcaag aggaagttcg agctatgcct
     2341 aacggaaaat gggaatctca cgtcaaggat cgtatcgaca ccatccgagc ctggaagcgc
     2401 agcggcttaa ccgacaagga aatcgccgag aacctcggca ttggctacac caccttccgg
     2461 cgctacatga aagatcacag gacactttgg tcagcattaa aaaccggcaa gtccgacgcc
     2521 gatgccttag tcgagaatgc cctttttaaa cgggctatgg gatacaggta tgaggaagtc
     2581 gaagaatctg tcaccaaaaa caaagaaggc gaaatcacag gcacccggac caagcgcatc
     2641 aagaagcaga tcgcgccgga tgtaacagcc atcattttct atctcaagaa ccgtaagtcc
     2701 caggattggc atgatcgcca tgcccttgag cattctggac cggacggaac ccctttaaat
     2761 ccccccgtga tacacgctta tatgcctccc aacggacgcg agctgctgcc tgaggagagc
     2821 gatgtacgat gaaaaaatgc ctgattttga ctgccatatt catgatcctc ggagccgcca
     2881 tggctgacca acgggaggtc atccgatatt ccccacagcc gggaccgcag gaagagtttt
     2941 tgaaaacacc tgcggacata gccatttatg gtggggctgc cggcggtggc aagacctggg
     3001 ctcttttgta tgaagcgctc cgctgggtcg aagtttcgaa ctatggtgcg gttatattca
     3061 ggcgggaata tactcagatc agggcagagg ggggcctgtg ggatgaatct ttgagcatgt
     3121 atccgcactg gcgcggggtg gccagagaag gttatctgga ctggcgcttc ccggccaggt
     3181 cgaagatccg ctttgcccac atgcagcatg aaaacgataa acataactgg gacggttcac
     3241 agataacgct gattggattt gaccaggctg agtccttcag ctggagccag atcgcttata
     3301 tgttcagccg aaaccgctcg acctgccgtg tcagccccta tatgcgcatg acctgcaatc
     3361 cggacccgga ctgctggctg cgggagtttc tgcgctggtg gatcgatgat gatacgggct
     3421 tcgccattga aaagcggtcg ggtaaaatcc gctacatggt caacgtgagc aatgagatcc
     3481 actggggtga cagtcccgag gagcttaaaa agaaattcgg gtcagattgt gatcccaaat
     3541 cctgcacctt tataccctct tcagtctacg acaacaaaat actgctcaaa cggaatcccg
     3601 gatatatagc caacctgcag gcccttcaga aggttgatcg cgaacgtctg ctccgtggca
     3661 actggaacat acgggagaca gccggcaatt ttttccagcg gccctggttt gaaattgtga
     3721 atgcggcccc cgccctggag gaggaaatac gctactggga cagagctgcg accgagacca
     3781 agccaggcca ggagaaaaag gcttcctgga ccgcgggcgt gaggattggc agggacagca
     3841 ggggaatttt ctacgtgacc gatatttgcc gcttccaggg gagccccatg aaagtcgagg
     3901 agactatcaa aaacgtagct tcccaggatg gccataaggt caaggtcggg atcgagcagg
     3961 atcctgggca ggccggtaag gcagaggctc tcggctacgc caggcttctg gccggctaca
     4021 gcgtggtttt aaatccggta cgcgaggcca agggcatccg ggcccggcca ttgtcggccc
     4081 aggccgaagc cggcaatatt aagctcgtga gagcgggctg gaatgatgcc tatctaaatg
     4141 aactcgagaa ctttgacggc tcagagggct gtgtgtcaga ccagaccgat gcatcgtcgg
     4201 gagcatttca cctattgaca aacattaagc aggctggcgt ctggggccgg tagagatggg
     4261 agggcgtgca gatggaacat gtgggatttt tgcaactttt gattagcttc ttatcgggat
     4321 tggtaggcgt cggtgtaggc tggggagccc tgcgaaatca agtcaagaac aatacccggg
     4381 atattgtcga gatctatagc cgactggcat cgatacatgg tcagaatagc gggcgttcga
     4441 tatttgtgcc cagggaggag tgcatggaaa ggcatgagga catgac
//
LOCUS       DSEB01000028            4485 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001687, whole genome shotgun sequence.
ACCESSION   DSEB01000028 DSEB01000000
VERSION     DSEB01000028.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001687"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1273
                     /locus_tag="ENO07_00525"
     CDS             <1..1273
                     /locus_tag="ENO07_00525"
                     /inference="COORDINATES: protein motif:HMM:PF00501.26"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HDS00477.1"
                     /translation="DYICAYLGLLLYGCTGILVDYRSRPDHLKYVYDNCEASLMVTPE
                     KRAGFEEIPGQLVFPSNLDEYPEIPENEIDTDWNPLALIMYTSGSTGIPKGVMLSHAN
                     LSHTISSIIEWAGIDENDRELTTLSLTHLFGLAHIHIYWTRGGTVYIEEKLNDIPDLL
                     NKIESERITSFPGTPGGFKLILDQFPEQFKEHCKNLKYIIVNSAPMDEVYIEKVLELL
                     PDTRFYMYYGLTEASRSTRICYNEHPDKLKSVGTPTPGSEVIVGTSGDRLIDQIGEIL
                     IKGPHVTSGYWGMDSSEFFDNGWFKTGDLGIMDADGFITWEGRLKEQINIDGLKLVPG
                     EVEMVLMTHERVLDCAVVGAPDELTGECVIGFAVVDAKPDKKLEIDLRKHCMRKLEVY
                     KIPRRIIFVDEIPKTDSGKVKRVLLKERLRR"
     gene            1270..2376
                     /locus_tag="ENO07_00530"
     CDS             1270..2376
                     /locus_tag="ENO07_00530"
                     /inference="COORDINATES: protein motif:HMM:PF13527.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDS00478.1"
                     /translation="MKGVKLRIRPATAGDYDAVANLLNRIFPPVDIEKRKRLWRWRHE
                     LNPARNSKIPPFLVAEKNERIAGVHGLVPMLFKVGNNTLTVVCSCDYAVQPDARSAGM
                     KLKLAAFSKEISPLHLSTSANRAANKITLALGGKELETGKLKLLNVLKYHRLIYSKLK
                     GKTGGGIARFLGTIMGGPLNLYSGLRGHTGKLEIDPAYKLEPVSAFDQKHDDFWNGIS
                     ADYDICVIRDSRYLNWRYIQYPFGKIQCRELLRDENVKGILAVHHSIDEDGLRFTAVL
                     ETLVSRNDNKSLNILLQQAVKAAVQNGSHYITSAPHDPQNLEYYRKIGFRIKTAEYSP
                     FTYKNNSPIGENFLADERRWYFSLGDGDICYYFE"
     gene            complement(2446..3537)
                     /locus_tag="ENO07_00535"
     CDS             complement(2446..3537)
                     /locus_tag="ENO07_00535"
                     /inference="COORDINATES: protein motif:HMM:PF01522.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00479.1"
                     /translation="MKAKVRRLVKFLISVMMYYSGILRLYSRVKPRNWFERNLKILLY
                     HRVLAEGDKKKAELQPGMCVLQSSFERQIRFIKRKFEIISVAELVAMLSRNEFPDKRI
                     ALVTFDDGWRDNYDYAFPVLQKYGSPATIFLATDYISCNNLPLIIEVNLLLSEADLWP
                     DLAIQIFETTVSESGQSGPDQSPGLPDNPKMRKDPFKFIKAVMGLDSGLQKIILDKMK
                     ARAALNKSDSNERWMLNKEEIKQMYASGIDFGSHGKSHQILTALDETELIRELEESKQ
                     KIENILNTEVISIAYPNGDYNSSVTRSARECGYKIGMAVSTDGGTADHPDIFAMKRIN
                     INEGSALGPRGRFSKAVFACLLEGLFQHF"
     gene            complement(3534..>4485)
                     /locus_tag="ENO07_00540"
     CDS             complement(3534..>4485)
                     /locus_tag="ENO07_00540"
                     /inference="COORDINATES: protein motif:HMM:PF01041.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00480.1"
                     /translation="PSAIVKAGLRIRLVDIDPRTLDYNYSQLEGVDSKDGLAVMACNL
                     FGILSDMHKLERIAGENQLYLIDDAAQSMGSRHGSKYSGTFGDAGIFSLGRGKNMTAY
                     EGGVLVTNNDRIAGKIDDSITELAKPSIKAEIKAFSNLLFYSLFLRPRLYWIPARIPF
                     LHLGETVFDPDFKLSQLSGIQKSVSALMFNELSGINSIRKKNALAIGQALIDNGSYMI
                     PGYTESNCPAYIRLPVICPNNSWRDRAIQKLVSRGINATIMYPCGIHQIPGIREHLGP
                     DCGHFPGAEMVADRLLTIPTHPYLKQTHIETIISTLAEMK"
BASE COUNT         1219 a         1057 c         1031 g         1178 t
ORIGIN      
        1 agattatatc tgtgcctatc tgggactttt actgtatggc tgcaccggca tcctggtgga
       61 ttacaggtcc cgtccggatc atctcaagta tgtctacgat aattgcgagg cctcactcat
      121 ggttactcct gagaaaagag ccggattcga agagataccc ggccagcttg tctttccttc
      181 aaatctggat gaatatcccg agattccgga aaatgaaatc gatactgact ggaatccttt
      241 agcgcttatc atgtacacca gcggctcgac tggaatcccc aagggggtca tgctctcaca
      301 tgccaatctc agccatacca tcagttccat aattgaatgg gctggaatcg atgagaatga
      361 ccgtgaatta accaccctct cattaacgca tctattcggg ctggcacata ttcatatcta
      421 ctggacgcgg ggcggcacgg tctatatcga agaaaaattg aatgatatcc ctgatctcct
      481 gaataagatc gaatcggaac ggataaccag cttcccgggt actcccggag ggtttaagct
      541 catactggat cagtttccgg agcagtttaa ggagcattgc aaaaatttga aatatatcat
      601 agtcaactcc gcgcctatgg atgaggtcta tatagagaag gttctggaac tgctcccaga
      661 tacccggttt tatatgtatt acggtctgac tgaggccagc cgcagcacca gaatttgcta
      721 caacgagcac ccggacaaac ttaaatcggt cggaacgcca accccgggat ccgaggttat
      781 agtcggcact tcgggggacc gactgatcga tcaaatcggg gaaatcctga ttaaggggcc
      841 gcatgttacc tcaggatact ggggcatgga ctcatccgaa ttctttgaca atggctggtt
      901 taagaccggc gacctgggaa ttatggatgc cgatggtttc atcacctggg agggcaggct
      961 gaaggagcag attaatatcg atggattgaa gctggtgccc ggtgaggttg aaatggtcct
     1021 gatgacacat gaacgggtcc tcgattgcgc ggttgttgga gctcctgatg aattgacggg
     1081 cgaatgtgtc ataggcttcg cggtcgtgga tgctaagccc gataagaagc tggagataga
     1141 cctgcgaaaa cactgcatga gaaaactgga ggtctataag atacccagaa ggattatatt
     1201 tgtggatgaa atccccaaga ccgattcagg caaagttaag cgggtgcttc tgaaggaaag
     1261 attgagaaga tgaagggcgt aaaacttcga atccggccgg cgactgcggg ggactatgat
     1321 gctgtagcca acctgctcaa tcgcatcttt ccaccggttg acatagaaaa gagaaaaagg
     1381 ctgtggcgct ggagacatga gctaaatccc gctcgcaata gcaagatacc gccgtttttg
     1441 gttgccgaga aaaatgaaag aatagcgggt gttcatgggc tcgttccgat gctctttaag
     1501 gtcgggaata acactctgac agtcgtctgc agttgcgatt atgccgtcca gccggatgcc
     1561 cgatccgcgg gaatgaaact gaaacttgcg gctttttcaa aggaaatatc tcccctgcat
     1621 ttatcaacct ctgccaatcg tgcggcaaac aaaatcacac tggccctggg cggaaaagaa
     1681 ctggaaacgg gaaaactgaa acttctgaac gtcctgaaat atcatagatt gatttattct
     1741 aaactgaaag gcaaaaccgg cggcgggatt gcaaggtttt tgggcacaat tatgggggga
     1801 cccttaaact tgtattccgg cctgaggggc cacacgggaa agctcgaaat agaccccgca
     1861 tataagctcg agcctgtttc ggcattcgat caaaaacatg atgatttctg gaacgggata
     1921 tccgccgatt atgacatatg cgttataaga gattcccgtt acctcaactg gcgctatatc
     1981 cagtatccat tcggaaaaat ccaatgccgg gaattgcttc gtgatgagaa tgtcaaaggt
     2041 attttggctg tgcatcacag tattgatgag gacggtcttc ggttcactgc cgtgctggaa
     2101 acactggtca gcaggaatga taataagagt ttgaacatcc tgcttcaaca ggctgtcaaa
     2161 gctgcagtac aaaatggatc acattatatt acctcagctc cccatgatcc tcaaaacctt
     2221 gagtattatc gtaagatcgg attcagaatc aaaaccgccg aatattcccc cttcacatac
     2281 aaaaataact cccctatcgg cgaaaacttc ctggccgatg aacgcaggtg gtatttctct
     2341 ctcggcgacg gcgatatctg ctattacttc gaataaataa atatctgatt attcaggata
     2401 ttgattcaag ctttcttcac actttgggga agatgaccgc aaatcttaga agtgttggaa
     2461 aagcccttcc agaaggcatg caaacacagc cttggagaac ctgccccgcg gccccagagc
     2521 tgagccctca tttatgttta ttcttttcat cgcaaatatg tccggatgat cggcagtccc
     2581 cccatcagta cttacggcca ttccaatttt gtaaccacac tcccttgctg accgcgttac
     2641 cgaagaatta tagtcaccat tgggataagc aatcgatata acctcagtat tcaaaatatt
     2701 ttcgattttc tgttttgatt cttccagctc tcttatcagt tctgtttcat cgagcgctgt
     2761 aagaatctgg tgcgacttgc cgtgtgagcc aaagtctatg ccggaagcat acatctgctt
     2821 tatctcttct ttattcagca tccacctctc attagaatcg gatttattca gcgcggccct
     2881 ggccttcatt ttatccaaaa ttattttttg caggccggaa tccagaccca taaccgcttt
     2941 aataaattta aatggatctt ttctcatctt cggattatcg ggaagccccg gactttggtc
     3001 gggtcctgat tgtccacttt cgctgaccgt agtctcaaaa atctgaatcg caaggtcggg
     3061 ccacaagtcc gcttcggaga gcagcagatt aacctcgatt ataaggggaa gattgttgca
     3121 ggatatgtaa tccgtggcaa gaaatatggt tgccggagaa ccgtattttt gcagtactgg
     3181 aaacgcataa tcatagttat ccctccagcc atcatcgaat gtgaccagtg caattctttt
     3241 gtccggaaat tcatttcggg aaagcatcgc gactaattcc gcaacagaga taatttcaaa
     3301 cttgcgcttt ataaagcgga tctggcgttc aaaagaactc tgcaagacgc acatgcccgg
     3361 ctgaagctct gccttcttct tatccccctc ggccagtacc ctgtgataca ataaaatctt
     3421 caggttgcgc tcaaaccaat tccttggttt aacacgggaa tacaaacgca gtattcccga
     3481 ataatacatc atcaccgata tcaggaactt aaccagtctt cttacttttg ctttcacttc
     3541 atttctgcca gagttgaaat tattgtttca atatgtgttt gctttaaata gggatgagtc
     3601 ggaatggtca agagccgatc cgcaaccatt tcagcgcccg gaaaatgtcc gcaatctggc
     3661 ccgagatgct ctcgtattcc cggaatctgg tgaatcccgc atggatacat tattgtagca
     3721 ttgattcccc gcgaaactaa cttctgaata gcccgatccc gccatgaatt attgggacaa
     3781 atgaccggaa gacgtatata tgcaggacaa ttactctccg tataacccgg aatcatatag
     3841 ctcccgttat cgataagagc ttgaccaatg gccagtgcat tttttttgcg aatgctatta
     3901 attcctgata gttcattaaa catcaatgcc gaaacgcttt tctgaatacc ggacaattgg
     3961 gacagcttaa aatcgggatc aaacacagtt tcaccgagat gcagaaaggg gattctggcg
     4021 ggaatccagt aaagccgcgg ccttaaaaaa aggctgtaaa acaagaggtt actgaatgct
     4081 ttaatttcag ctttaatgga tggtttcgcc agctcagtta tggaatcgtc tattttcccg
     4141 gcgattcggt cgttatttgt aaccagcaca ccgccctcgt aggcggtcat gttttttccc
     4201 ctacccaaac tgaaaattcc tgcatccccg aatgttccgg aatactttga accatgtctt
     4261 gaacccatag actgggcggc gtcatcgata agatataatt ggttctcgcc ggctattcgt
     4321 tccagtttat gcatatcact gagaatgccg aacaggttgc aggccatgac agcaagccca
     4381 tctttgctgt cgacaccttc aagctggctg taattataat ccagcgtacg ggggtcaata
     4441 tcaacaagtc tgatcctgag gcctgctttc acaatagcgg atggc
//
LOCUS       DSEB01000029           10301 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000285, whole genome shotgun sequence.
ACCESSION   DSEB01000029 DSEB01000000
VERSION     DSEB01000029.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10301)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10301)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10301
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000285"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(133..711)
                     /locus_tag="ENO07_00545"
     CDS             complement(133..711)
                     /locus_tag="ENO07_00545"
                     /inference="COORDINATES: protein motif:HMM:PF13420.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDS00481.1"
                     /translation="MRAIMPVMLPTLKGRKINLRRVHKSDAESIYKHAGDEEISRYTF
                     LPNPYRLDDAYRFIAVTHTALRAKKAFNFGIEYKEEKEIIGMISLEHVNFQHLFAETG
                     YWLGRKYWSQGIAREALQLVLKFGFEQLKLNRIYARVMHPNIASARVLEKTGFKKEGI
                     MRKSIWRGGTWMDLIWYAILKEEYKHIKADWK"
     gene            complement(830..1768)
                     /locus_tag="ENO07_00550"
     CDS             complement(830..1768)
                     /locus_tag="ENO07_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDS00482.1"
                     /translation="MVELKKTRVGFGHGLVDAGRKSEKVVVLVGDLRDSTNVNFFAEE
                     FPQRFIEVGIAEQNMANVAAGLSLVGKIPFFTTYGAFATCRCLDMLRVTICYSNLNVK
                     IGGAHGGISVGPDGATHQALEEISILRSLPNMKVIVPCDYYETRKATLASIDIKGPVY
                     IRFGRENVPVVTGEKTPFEFGKGALYRDGGDVAILACGVMLYEALAAADELQRDGIDA
                     RIINIHTVKPIDEDIIIKAASECGAIVTAEEHQVYGGFGSAVAEVIVKNSPVPMEFVG
                     IQDSFGRSGQPEELMQTYHLKAVDIIKAAMKVLGRK"
     gene            complement(1761..2624)
                     /locus_tag="ENO07_00555"
     CDS             complement(1761..2624)
                     /locus_tag="ENO07_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDS00483.1"
                     /translation="MNPFNFDIEQLNQKALDIRRDIITLLVESQSGHSGGPLSCADMA
                     AALYFNILNHNPGNPDDPVRDLNFFSIGHVSPLIYSVLAEAGYFPLRDLLTFRKLNSH
                     LEGHPNCLHTPGIEVSAGSLGQGLSIACGAAYGIKMDGSERSVFCFMGDGEQQEGSIW
                     EAVMFAAHYRLDNLCALIDYNHRQIDGETEKVMNIAPLAEKYWSFGWNVYEIDGHNMG
                     EILGALKACSEFTGKPSVIIGHTVMGKGVSFMEGLAEWHGKPPAPDQGEKALAELGTT
                     FREWSERLRNG"
     gene            complement(2740..3342)
                     /locus_tag="ENO07_00560"
     CDS             complement(2740..3342)
                     /locus_tag="ENO07_00560"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:PRK00081.3-2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDS00484.1"
                     /translation="MIKLGITGQIASGKSTVAREFAKFGGRVIYADEIGREVVEERPP
                     ILSKLIRAFGPQIVTPQGILKRRELGRIVFASAEKREILNKIVHPPLLQRLREELDKC
                     SLDPECKLAIVDAALLVDWGWHEKMDWTVCVVTAPDTQILRLKTKGLTDQEIRDRINS
                     QTNREELISAADFVIENDGSLEELKQKVEMIYDQIMLESK"
     gene            3995..6877
                     /locus_tag="ENO07_00565"
     CDS             3995..6877
                     /locus_tag="ENO07_00565"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00593.22,HMM:PF13715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDS00485.1"
                     /translation="MFKHKLILYLLTAAVALLLLSSTAFAANVGKVAGRVVDRETGEG
                     LPTVAVQLVGTNMGALTDVDGQYTILNVPVGEYDIKAELVGYQPIEKRGIFVSVDLTT
                     YVDFELTSRAIDVGEVQVVEAERPLVIRDQTSTLRLVSGDEIQNLPTKDYQDVIGFST
                     GVVTYQDNFGIRQRGGDETSNNPTLNIRGGRQNEVAYMVDGFDTKDPLTGLANLSINN
                     NSIEEISITTGGFNAEYGWIASGAVQVTTKEGIRNYSGSVEAITSNFTDDYGYNSYSV
                     DFTGPILPDNEKLSFFVSGERRVEDDRSPHALNDGDRLPNNDLRGWTWQGKLRYDVTP
                     NFIARIGILGSYDEFDRYDRSFHFNQEHMAKFEDRNNSFYLQFTHNVSKKTFWTAKAN
                     YFETYRFLGDGVFFDDPWAYASELNPNFNDTKLFWPGDDPETEEDEAHFFDDFTKRES
                     AYYGAEFDITSQINAENELKFGLEYQRHTLRWLRHATPRRLYQGPEGGLDDVDNYGYD
                     IVFQYDADSNIVGASIEDLDDGWNAAKHPYTFAAYLQDKFEWEGLVVNAGLRYDYLNV
                     NTDRLINDEIPLDPFERALDPDATDEEIQMAQELNPEDLEPAEAEQRLSPRLGIGFPV
                     TDKTVFHLYYGKFFQRPELQYLYTSYDYLEYMVREAPYYNPVGNPNLEPEETTAYEVG
                     VTHQLGDNARFDMTAYYKDIKNLTQVINQPSQPNAFATYRNQDYGTVKGLEFAMKMRR
                     TRNIAFDIAYSLQYANGTGSFATSQTNIAWTNSERPRRVSPLEFDQRHKITAIVDVRS
                     GRGEGPRLGDFYPLEKSGINAVLTVGSGLPYSPSEIFNEVTLAAVSPNPAAPINSRYG
                     PWTYRVDLKASRSFNIASLDLNFYVWVLNVFDTENAIAVYEGTGKPDDTGWLATEPGQ
                     VFIDTYSEADPNYGNMNGLEQYEFRQRDPRNYDIPRMIRLGMRLSF"
     gene            6901..9969
                     /locus_tag="ENO07_00570"
     CDS             6901..9969
                     /locus_tag="ENO07_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00486.1"
                     /translation="MRLIKTFIFLVCLAMLTSVAWGVSSQTGKVQSSNQALILDTDTY
                     LDVNNLLCFVYNDGNYAYDNANILGKTDGLYFPRGTKRTVIYDAGIWVGAKVEDDLRV
                     VVAEYGSEFVPGPMENGTFIPDNASFRVYKINRGDNAETNPDWANWPVDQGAPVDSLG
                     NPALLGDQMCWAVYNDADASSHNIDAGASLPLGIEIQQSTFAYGRSGALGQVIFMKFL
                     IINKGGNQLDSTFISLWADPDLGDAGDDLVGCDTILSMGYCYNEAGGDAIYGQTPPAV
                     GFDFFQGPIIETGNMDDSAKFQGQWLYGYVNLPMTSFNKYINGTDPHSPLETYNYQRG
                     LTIDGQDQVNPTTSEVTKFMHSGDPVTATGWLDVAADDRRYMMTTGPFTMLPGDTQEV
                     VAAIVVGQGGDRLSSITEMKTVDEQAQVVFDLNFDIPSPPPAPVVHARGYDGAIDLTW
                     TPADPEQNWPEAHFQDYIETLNEFYVFEGYNIYQGLSPSGPWTKVATYDYDGATSMHT
                     FIDSAGEYIYFNCEDEVCDSALRPWDFQLIYSDVVNNQAGGVEKVITQAGSESGLVNH
                     LFIERSYLDGGPIINNRPYYFAISPYSVNIQEVLSEDSIFSGVNFLGFNAASLEGTRV
                     PITVVPKSSGAVFVDTADHNQGPSQGMVVVEYLNYTNDEPEDFTVTFNEDGTWNLLRE
                     GATVLSNQDNQGGGYDYDVIDGLMVRVMGPDPGILSIVETAFAGDPVSPPDNLFWSLS
                     STGDYYVSSDVSGSSQAAKDRFNWRGLMEWESWEFRFTDEGSEYYNWNDDEKFPDRAP
                     FEIWHFSENETEPDRRDIFFILDDDGSGGWSYGDRIYVSETDYPSEPLPQNAGATGYV
                     WPDHFHLGRVIFNDFSGALTAPPSGTVVRFNSTIPNTQLDQFQFTTKPTGMQDGTVVE
                     LSLDDVKVVPNPYYNYSILEPDQFDRIIKFTNLPPTRCTIRIFNIAGDLVREMVKEDE
                     AAAEFVWDVKTNSGLYVASGIYVYYIEAEGIGDTFGKMIVFTEVEQLNTY"
     gene            10022..>10301
                     /locus_tag="ENO07_00575"
     CDS             10022..>10301
                     /locus_tag="ENO07_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00487.1"
                     /translation="MLLCSAALYAGGDERIGTSGAQELRIPVGSRMSALAGAGVAEAV
                     GAEALYWNPAGVAYYDGTEVMFTHLQYFADIDVNYFAATTAIEDFGSIG"
BASE COUNT         2446 a         2573 c         2641 g         2641 t
ORIGIN      
        1 ctcgcatccg gtgtatatct ttattacgtg attaccgacg gtacgacagt ttccggtaaa
       61 cttgcgatta taaaagaata gaggcagttc gtgggtattc cgtgtatgaa aaggccagcg
      121 gatggccttt ttttatttcc agtcggcctt tatgtgtttg tattcttcct ttaaaatggc
      181 ataccagatg aggtccatcc acgtcccccc gcgccagatt gacttgcgca taatcccctc
      241 ctttttgaat ccggtttttt ccagcaccct ggcggaggcg atattagggt gcattacccg
      301 ggcataaatc cgattcaatt tcagctgttc aaatccgaat tttaaaacta attgcagcgc
      361 ctccctggcg ataccctgac tccagtattt cctgcccagc cagtatcctg tctccgcaaa
      421 tagatgctgg aaattcacat gttccaggct gatcatgccg attatctctt tctcctcttt
      481 gtactcgatg ccaaaattaa aggccttttt ggcccgcagg gcagtgtgag tgacggcgat
      541 aaaccggtag gcatcatcga gccggtaagg attcggcagg aatgtatagc gtgaaatctc
      601 ctcatcaccg gcatgtttat aaatcgattc ggcatcggac ttatgcaccc ggcggagatt
      661 gatttttcgt ccttttaatg tcggaagcat tactggcata atggccctca tctgatactc
      721 taatcaatat tgcccggccg gttcttctat gtcaagcgct tagatccggg aagatccgct
      781 cgaccgatag aaagcgggca attacacctc cagtatatgc cgaaaatatc tacttgcgcc
      841 ccaaaacttt catggctgcc ttaattatgt ccacggcttt cagatgatat gtctgcatca
      901 attcctcggg ttggccggag cgcccaaagg agtcctggat gcccacaaat tccattggaa
      961 cagggctgtt tttaacaata acctcagcca cggccgagcc aaagccgcca tagacctggt
     1021 gctcctctgc cgtgacaatg gcgccgcatt cggaggcggc tttgatgatt atatcttcgt
     1081 cgatgggctt gaccgtgtga atgtttatga tacgggcatc gattccatcg cgctgtaact
     1141 catcagcggc cgccagggcc tcatagagca tgactccgca ggccagaatg gcgacatctc
     1201 cgccgtcacg gtacaaagcc ccttttccga attcaaaagg tgttttttcg ccggttacaa
     1261 cggggacatt ttcacggccg aaacggatat aaacgggtcc tttgatatct atcgaggcca
     1321 atgtcgcctt gcgggtttca tagtaatcac aaggcactat gactttcatg ttgggaaggg
     1381 aacgcaggat cgagatctcc tccagggcct gatgggtggc tccatccggg ccaaccgaga
     1441 tgccgccatg tgccccgcca attttgacat tcagattgga gtagcagata gtgaccctga
     1501 gcatatccag gcagcggcag gttgcaaagg cgccgtatgt ggtgaagaaa ggaatcttgc
     1561 ccacgagcga tagtcccgcg gcgacattgg ccatgttctg ttcagcaatt ccgacctcga
     1621 tgaatcgttg cgggaattct tccgcaaaga aattgacatt ggtggaatcg cgcaggtctc
     1681 ccaccagcac cacaaccttt tcacttttgc ggccggcatc caccagccca tgcccgaatc
     1741 ccacccgcgt ttttttcagc tcaaccattg cgaagcctct cagaccattc ccggaacgtc
     1801 gtgcccagct cagccagcgc tttttctccc tggtccggtg cgggaggctt gccgtgccac
     1861 tccgccaggc cctccatgaa tgaaactccc ttacccatta cggtatgacc gattatcact
     1921 gacggcttgc cggtgaattc agagcaggcc ttcaatgccc ccaggatttc tcccatattg
     1981 tgtccgtcga tctcatagac attccatccg aatgaccagt atttttcggc cagcggggcg
     2041 atattcataa ctttctctgt ctcgccgtca atctggcggt ggttgtaatc aatcagggca
     2101 cagagattgt cgagcctgta atgggcggcg aacatgaccg cctcccagat cgagccctcc
     2161 tgctgctcac cgtcgcccat gaagcagaag acgctgcgtt cactgccgtc cattttgatc
     2221 ccataggccg ccccacaggc gatcgacagg ccctgcccca aagagccggc ggaaacctcg
     2281 atcccggggg tatgcaggca gtttggatgc ccctccagat ggctattgag ctttctgaat
     2341 gtgagcagat ccctgagcgg aaaataaccg gcttcagcca gcacagaata aatcaggggg
     2401 ctgacatgcc cgatagagaa gaaattcaga tcgcgcacgg gatcatccgg attacccgga
     2461 ttgtgattga gaatgttaaa atacagggcc gccgccatat ccgcgcagga cagcggcccg
     2521 ccggaatggc cgctctggga ttcgaccaaa agggtaataa tgtcgcgcct gatatccagc
     2581 gccttctgat tcaactgctc aatatcaaaa ttgaaaggat tcaattgaca ccctgataat
     2641 aataacaagt gaataagtgc caaaaatagg ggagaagagg agcattgtca atttttttat
     2701 ctgcggtgtt tcaggcagac gggcgaactc gaccctctgt tatttggact ccagcattat
     2761 ctggtcgtaa atcatctcga ctttttgctt gagctcctcc aggctgccgt cattttcgat
     2821 aacaaagtcg gccgcggaga tcaattcctc gcgatttgtc tgggaattta tgcggtcgcg
     2881 tatttcctga tcggtaagtc ctttcgtttt taagcgcagt atttgcgtat ccggcgcagt
     2941 gaccacgcac acagtccagt ccattttttc atgccagccc cagtccacca gcagggcggc
     3001 atcgacaata gccagtttgc actcgggatc aagcgagcac ttatccaatt cctccctgag
     3061 tctctgcagc agaggaggat ggacgatttt gttcagtatt tccctttttt cggctgaggc
     3121 aaagacgatt ctgcccagtt cccttctttt cagaatccct tgtggggtaa cgatttgggg
     3181 gccaaaggcg cggatcagct tggaaaggat ggggggcctt tcttcgacaa cctcccggcc
     3241 tatctcatcg gcatatatta cccttccgcc gaatttagca aattcccttg cgaccgtgga
     3301 tttaccggag gcaatctgtc cggttatacc gagttttatc atacatattc ctatatgcta
     3361 caaatagtta aattgttgca tttatttttt tgtccgcctc tgaaggtgaa ttattttcat
     3421 aatcttccga tctaaattta agaaatatta aggcatttgg caaataaagc gaattatctg
     3481 tgtcgtatat tatttggcac tggggtaatt cttacagctt tatgtaccca atccataagg
     3541 cgaatcatta tttttttatc ttgactttat ttattaaatt tttgtaaact cccatagaag
     3601 atatggggga taagccctgg ggcagatcgt ttttcagggc tttttgggga aggctgtgta
     3661 gaaggtatga tggtcatgga tgacaaccat cctctaagga ttgggaagtt acaaccgcac
     3721 ggtggccgcg gatcgggggc cgataatacc tgttttaaaa atgcaagaga gcgttatgta
     3781 catggaggat ttcgagcaaa ggaggtgtgt ataggctttc gttacagatc ttacccaaaa
     3841 aatcctatct tttcaacctg ggcattttga tttagataaa aatgttcgat ggtgaagttt
     3901 gtccaatttg taacaaggag gtgtgaagta cctaaaattg ttaatgtata tcttataagt
     3961 tttttcccta acaacaaagt gaggaggttt caccttgttt aaacacaaac tgattttata
     4021 cctgctgact gcggcggtcg cattactgct tctgtcatcg acagccttcg cagccaatgt
     4081 aggtaaggtc gcgggcaggg tagtggaccg tgagaccggg gaaggcttac cgacagtagc
     4141 cgtccagctg gtaggaacca acatgggcgc cctgactgat gtcgacgggc agtatacgat
     4201 tctgaatgtg cccgtaggcg aatacgacat caaggctgag ctggtcggtt atcagccgat
     4261 tgagaagcgc ggcatctttg tctccgttga cctgaccacc tatgttgact tcgagctcac
     4321 ttccagggct atcgatgtcg gcgaggttca ggttgtcgag gccgagcgtc cgctggtcat
     4381 cagggaccag acttcgaccc tgcgcctggt ttcgggagat gagattcaga atcttccgac
     4441 caaggactac caggatgtta ttggtttctc caccggcgtg gttacatacc aggacaactt
     4501 cggtatacgg cagcgcggtg gagacgagac gtccaataac ccaaccctga atattcgtgg
     4561 tggccgacag aatgaggttg cctacatggt tgacggtttt gacacaaagg acccgttgac
     4621 cggtctggct aacctcagta ttaataacaa ctcgatcgaa gagatctcga ttacaaccgg
     4681 tggattcaat gccgagtacg gctggattgc ctcgggtgca gttcaggtga caaccaagga
     4741 aggcatcagg aattactccg gtagtgtcga ggcgattacc agtaatttta ccgatgacta
     4801 tggctataac agctattcgg ttgattttac cggtccgatt cttcccgata acgagaagct
     4861 ttcattcttc gtttccggcg aacgccgggt ggaggatgat cgttctcctc atgcgctgaa
     4921 cgatggcgat cgtctgccca ataacgatct gcgtggatgg acctggcagg gtaaattacg
     4981 ttatgatgtt acccccaatt tcatcgcgcg catcggaatt ctgggctcct acgatgagtt
     5041 cgatcgttat gatcgttcat tccactttaa ccaggagcac atggccaagt tcgaggatag
     5101 aaacaattca ttctatctgc agtttaccca taatgtcagc aagaagactt tctggacagc
     5161 caaggccaac tactttgaaa cctacaggtt ccttggcgac ggcgttttct ttgatgaccc
     5221 ctgggcctat gcttccgagc tcaaccctaa tttcaacgat accaagctct tctggccggg
     5281 cgacgatccc gaaaccgagg aggatgaggc tcacttcttt gatgatttca ccaagaggga
     5341 atcagcctac tacggcgctg aattcgatat tacatcgcag atcaatgccg aaaatgaatt
     5401 gaaattcggt ctggagtatc agcggcatac attgcgctgg ctgcgccatg ccacccccag
     5461 aaggctctat cagggtcccg aaggcggcct ggatgacgtg gataactatg gatatgatat
     5521 cgtgttccaa tatgatgcag attcgaatat agtcggcgca tccatcgagg atctggatga
     5581 cggctggaac gccgccaaac atccctatac ctttgctgcc tacctccagg ataaattcga
     5641 gtgggagggc ctggtggtca atgccggttt acgttatgat tatcttaatg tgaacactga
     5701 ccggcttatc aatgatgaga tcccgcttga tccattcgag agggctctgg atcctgatgc
     5761 caccgatgaa gagattcaga tggcgcagga gcttaaccct gaggatctgg aaccggccga
     5821 ggccgaacag agattgtcgc ctcgtctggg tatcggattc ccggtaacgg acaagacagt
     5881 cttccatcta tactatggca agttcttcca gcgccctgag ctccaatacc tgtacacgag
     5941 ctatgattat ctcgagtaca tggtgcgcga ggctccctat tacaatccgg ttggaaatcc
     6001 gaatcttgag cccgaagaaa ccacggccta cgaggtcggt gtaacccacc agctcggtga
     6061 taatgccagg tttgatatga cagcctatta taaggacatc aagaatctta cccaggttat
     6121 caaccagccc tcacagccga atgcttttgc tacgtatcgc aaccaggatt acggcacggt
     6181 caagggtctg gaatttgcaa tgaagatgcg gagaacccga aatatcgctt tcgatattgc
     6241 ctattctctg cagtacgcca acggtaccgg ctcatttgcc acttcccaga ccaatattgc
     6301 ctggacgaat tcagagcgtc cgcggagagt atctcccctg gagttcgatc agaggcataa
     6361 gattacggcc atcgtggatg ttcgcagcgg tcggggcgaa ggccccaggc tgggcgattt
     6421 ttatcctctg gaaaagtccg gaatcaatgc ggttctcaca gttggatcgg gcctgccata
     6481 ttcgccttca gagatcttta acgaggtcac actggcggcg gtatcgccta atccggccgc
     6541 tccgattaac tcccgctacg gcccctggac ctacagggtg gatctcaagg ccagcaggtc
     6601 cttcaatatt gccagccttg atctgaactt ctatgtctgg gtacttaatg tctttgatac
     6661 cgagaatgca attgcggtat acgagggcac cggtaagccg gatgacaccg gctggctggc
     6721 aaccgaaccg ggacaggtat ttattgatac ctatagcgaa gctgatccga attacggcaa
     6781 tatgaatggt ctggaacaat atgagttccg ccagagagac ccgagaaact atgatattcc
     6841 tcgtatgatc cgtctcggca tgagactcag cttttaaatg ggaagtttaa ggaggaaagg
     6901 atgcgattaa taaagacctt tatctttctc gtctgtctgg cgatgctgac ctcagtcgca
     6961 tggggtgtgt cgagtcagac cggaaaggtt cagtcgtcga accaggccct gattttagat
     7021 accgatacgt atctggatgt taacaacctg ctttgcttcg tctataatga cggaaactat
     7081 gcatacgata atgctaacat cctgggcaaa actgacggtc tctatttccc gcgcggaacc
     7141 aagagaaccg ttatttacga tgccggtatc tgggtggggg caaaggttga agacgacttg
     7201 agagttgttg tggcggaata cggtagtgaa tttgtgccgg gcccgatgga gaacggtact
     7261 ttcattcccg ataacgccag cttcagagta tataaaatca accgcggtga taacgccgaa
     7321 acgaatccgg actgggccaa ctggcccgtg gatcagggcg ccccggttga ttcattaggc
     7381 aatcccgcac ttctgggtga ccagatgtgc tgggccgttt ataacgatgc tgatgcatca
     7441 tcacacaata tcgacgctgg agcctctctg cccctgggca ttgaaattca gcagtcgacc
     7501 tttgcttatg gccgttccgg ggcattggga caggtcatat tcatgaagtt cctgataatc
     7561 aataagggtg ggaatcagct cgatagcacc tttatttctc tctgggccga ccccgacctt
     7621 ggagatgcgg gtgatgacct ggttggatgc gatacgattc tatccatggg ttattgctat
     7681 aacgaagccg gcggcgacgc catctatggc caaacgccac ctgcagttgg tttcgacttc
     7741 ttccagggcc cgatcatcga gaccggcaac atggacgatt cggctaagtt ccaggggcag
     7801 tggctttatg gctatgtgaa cctgcccatg acttcattca ataagtacat caatggaact
     7861 gacccgcata gtccgctcga aacctataac taccagcgcg ggcttacaat cgacggtcag
     7921 gatcaggtca atcccacgac ctcagaagtt accaagttca tgcattccgg tgatccggtc
     7981 accgctacag gctggctgga tgttgcggct gatgaccgcc gttacatgat gacaaccggc
     8041 ccgttcacta tgcttccggg agacacccag gaagttgtgg cggccatagt tgtgggccag
     8101 ggcggcgatc gtctttccag catcactgag atgaagactg tcgacgagca ggcccaggtt
     8161 gtatttgatc ttaacttcga tattccgtct cctccgccgg cccctgtggt tcatgcccgc
     8221 gggtatgacg gcgctatcga cctgacctgg accccggccg atcctgaaca gaattggccc
     8281 gaggcccatt tccaggatta tatcgagact ctgaatgaat tctatgtctt tgaaggatat
     8341 aatatttatc agggtctatc gccctcgggt ccgtggacca aggtggccac atatgactat
     8401 gatggtgcaa catccatgca taccttcatc gattccgcgg gtgagtacat ctatttcaac
     8461 tgcgaggatg aggtatgtga ttcggctctg cgtccctggg acttccagtt gatctattcc
     8521 gatgtggtta ataaccaggc cggaggcgtg gagaaggtca ttacccaggc cggaagcgaa
     8581 agcggtctgg tgaaccatct cttcatcgag agaagctatc tggatggtgg accgattatc
     8641 aacaatcgtc cgtactattt tgcaatttca ccttattcgg tcaatattca ggaagttctt
     8701 tctgaggatt ccatcttcag cggagtaaac ttcctgggct tcaatgccgc atccctggaa
     8761 ggaaccaggg tcccgatcac ggttgtgccc aagagttcgg gggctgtatt tgtcgataca
     8821 gcggaccata accagggtcc atcccagggt atggttgtgg tcgagtatct caactacaca
     8881 aatgatgagc cggaagattt cacggtgacg ttcaatgaag acggcacctg gaatctgctg
     8941 cgcgaaggag ctacggttct ttccaatcag gataatcaag ggggcggtta tgactatgat
     9001 gtcatagacg gtctcatggt tcgtgtgatg ggccccgatc ccggtattct ctctatagtt
     9061 gagacagctt ttgccggtga tccggtttcg ccgccggata atcttttctg gtcattgagt
     9121 tcaaccggtg actactatgt ttcatctgac gtgtccggca gcagccaggc ggccaaagat
     9181 cgcttcaact ggcgcggctt gatggaatgg gaatcctggg aattccgctt taccgatgaa
     9241 ggaagcgagt attacaactg gaatgatgac gagaaattcc ctgatagggc cccgtttgag
     9301 atctggcact tctcggaaaa tgagaccgag ccggatagaa gagacatctt cttcattctt
     9361 gatgatgacg gatctggcgg atggagctat ggagacagga tctatgtgtc ggagactgac
     9421 tacccgtccg aaccgcttcc ccagaatgct ggagcaacag ggtatgtctg gcccgatcat
     9481 ttccatctgg gacgtgtgat atttaatgat ttcagcggag ctcttactgc gccgccatct
     9541 ggtacggtcg tcaggttcaa ttctacgatt ccgaatactc agcttgacca attccagttt
     9601 accaccaagc cgaccggcat gcaggatgga accgttgttg agttgagtct ggacgatgta
     9661 aaggtcgttc cgaatccata ttacaactac tcgatcctgg agccggatca gtttgatcgt
     9721 attattaagt tcacaaatct gccgccgacc aggtgcacaa ttagaatctt taacatcgcc
     9781 ggcgaccttg tccgcgagat ggtcaaggaa gacgaggctg ccgcggaatt cgtctgggat
     9841 gttaagacta acagcggcct gtacgttgcc tccggtatct atgtgtatta catagaggct
     9901 gaagggatcg gcgacacatt cggtaagatg attgtgttca ccgaggtcga acagcttaac
     9961 acttattagg cggggaggaa ttataatgaa gataaaagta ctttgcttaa cgttagttgc
    10021 aatgcttttg tgctctgcgg ccctctacgc cggaggtgat gagcgtattg gtacctcggg
    10081 tgcacaagag ctgagaatcc ctgtcggcag tcgcatgtct gcgctggcgg gtgcgggagt
    10141 ggcggaggca gttggtgccg aagcgctcta ctggaacccg gcgggcgttg catactacga
    10201 cggtactgag gttatgttca cgcatcttca gtacttcgcc gatatcgatg tcaactattt
    10261 tgccgcaacc acggcaatcg aggacttcgg ttctataggt c
//
LOCUS       DSEB01000030            5177 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001208, whole genome shotgun sequence.
ACCESSION   DSEB01000030 DSEB01000000
VERSION     DSEB01000030.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5177)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001208"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            36..3659
                     /locus_tag="ENO07_00580"
     CDS             36..3659
                     /locus_tag="ENO07_00580"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00512.23,HMM:PF01590.24,HMM:PF02518.24,
                     HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HDS00488.1"
                     /translation="MERCRKGIEEGGFEQPPHFMKCIDENRIIHIKDLDVDPLWPKEE
                     GRAIKTKALVIAPIRIKDEPIGVIAGNLEHHEREIDDQDVARFGMFANMVGLALDNIR
                     VYRSLERKVEERTRSLRAAYSELDEKANLLSKSRDQLQAILDSSIPAMIMVDERNVVT
                     AANRRVSEFFGLNPDSLMDHPLDRFHEQIAGCFNDPDRHKDLIRELSRRSDVETKHML
                     DASVMTERTLELVRPHPRYVSVISVPVLDREDRELGRVWIYVDITDVRKAEEQVRLIV
                     DNSPTPTIISRLEDGKIIYVNDQLAELMGYSREEVMEKPTTDFYYRKEQREEVVQRLK
                     TDGRLRDFEMRFVRKDGAVLWITLNLVATQFRGEKVIIGGLSDITSRKKAEEELRKER
                     NFISAILNTAGALVVVLDPDGKILRFNKACERVSGYKPEEVIGKNFADIFILPEELAM
                     LAERFEIIKKSRGRVDGDNYWITKDGQKRMISWSNNVMLDENGDVEYVIATGIDITER
                     KAAEDKLRLYRELYLNSKDGIVIVTKEGLYQESNPAYLEQTGFDSADLKGMKVSELMG
                     IPDPEKFQKELLEKGGFRGEMILRTRDGRELPFDVSVFTIEDSKEEIVNLAGIGRDIS
                     EQKAAQAALSKRVWYEEGLAACSQALLTTANFDDALREALFHLLVASRSSRAYIFENF
                     ESPEDGLCMRQTHEVCDTGIEPQIENQELQHFPYSRGFQRWCEILGRGEAISGPVAQF
                     PKAERELLESQGIRSIIVFPITVEGRWYGFVGFDECRQEREDTEEDLRLLRTASEMIG
                     SFVESRKFEENLRVSEQRFRSLVENASDVIYSISPDGKFTYISPQFTESTGYDVAEYI
                     GKPSTLLLSDEQIEDSRSWFNAGMPDHDDHVEGYQFKMETKNGDLRWFTAHTAIIRDD
                     SGNVIEGIGIAHDITEIKKLLESLEKTNRELVNTQAQLVQTEKMASLGQLVAGVAHEI
                     NTPIGAVNSMHNSLIRAVERLSKILRDKYPDEIADGGDLQKYLHVIAEANRIIASGTE
                     RVTTIVRRLRSFARLDEAELKTCDIHEGLEDTLTLIHHEVKHHIEIVRDYGDLPEFAC
                     YPGRMNQVFLNILNNARQAIRDKGQITIRTYLKDNLAHIEISDNGVGISRQNLKKIFD
                     PGFTTKGVGVGTGLGLSICYQIISGHYGDIEVESEPGIGTTFTIKLPLNLDDILEKQR
                     NEN"
     gene            4179..5135
                     /locus_tag="ENO07_00585"
     CDS             4179..5135
                     /locus_tag="ENO07_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00489.1"
                     /translation="MHLPINVLEGDGPELKESYDVGSTYPVFILTDSEGEIIIRWTGY
                     STASQFVYKLNASLKDLRTIKERRAQFEANPTLQEALALGRFYAEIGEHIEAVAMFRR
                     AEKLSAQRKINLSIDIFKNMAEAVWKEMAPYDSIFPAAEYVLASGGAPGIIKMATLMS
                     RLSRKFDTTDSLRKYLDASIQAASESKDPQHAEDRYSLVAEHALQIAHDTAKAIEIKE
                     LSMGAGWESNPTKFYGYAKWCLERKVNLGAAEMYARRAVQVAGSPEMRARVLNTLAEI
                     YEVRGNYEGAIQAIEMAIENHPENPYYQTRLDEIEDAIAGGQ"
BASE COUNT         1409 a         1149 c         1417 g         1202 t
ORIGIN      
        1 gatctgtgtg tttcccgaaa gacccgcctg aagtcatgga gcgttgcagg aaaggcattg
       61 aagagggagg attcgagcaa ccgcctcatt tcatgaagtg catagatgaa aacaggatta
      121 tacacataaa agacctggat gtcgatccgc tctggcccaa agaagaaggc agggctatca
      181 agacaaaggc tcttgtcata gcgcctattc gtatcaagga cgagccgatc ggtgtgatcg
      241 caggcaacct ggaacatcat gagcgcgaaa tagacgacca ggatgtggcc agattcggga
      301 tgttcgccaa tatggtcggc ttggccctgg acaacatcag ggtctatcgt tccctcgaga
      361 ggaaagttga ggagaggaca agatcacttc gtgcagctta ctccgagctg gatgaaaagg
      421 ccaacctgct ttccaagagc cgggatcagt tgcaggctat actggattcc tcgattccag
      481 ctatgattat ggttgatgag cggaatgtgg ttacagcggc taaccggcgg gtcagtgaat
      541 tcttcggtct caatccggat tcgcttatgg atcatcccct cgatcgtttt catgaacaaa
      601 ttgccggctg cttcaatgat ccggatcgtc ataaagatct gatacgggag ctttcccggc
      661 gatccgatgt cgagacaaag catatgctcg acgcctcggt tatgaccgaa agaacattgg
      721 aattggtgag gcctcatcct cgttatgtgt ctgttatcag cgttccggtt cttgatcggg
      781 aggatcgtga actgggcagg gtctggattt atgttgacat tactgatgtc agaaaagccg
      841 aggagcaggt gcgcctgata gtcgacaatt ctcctacgcc gacgattatc agcagacttg
      901 aagacggcaa aataatatat gtgaatgatc aactggccga gctgatgggg tattccagag
      961 aagaggtgat ggagaagccg acaacagatt tctattacag aaaagagcag agggaggagg
     1021 ttgtgcagag gctgaaaacg gacggccgcc tgcgcgattt tgagatgcgc ttcgtgcgca
     1081 aggacggcgc ggtgctctgg attacattga atctggtggc tacgcagttc aggggagaga
     1141 aggttataat cgggggtctt tctgatataa cctcgagaaa aaaagccgag gaagaacttc
     1201 gcaaggaacg taatttcatt tcggctatac tgaataccgc cggagccctg gtggtagtgc
     1261 tcgatccgga tggcaaaatc ctgcgtttca acaaagcctg tgaacgggtc agcggatata
     1321 aaccggaaga agtgataggg aaaaatttcg ccgatatttt catactgccg gaggagctgg
     1381 caatgctggc ggagaggttt gagattataa agaagagccg cggccgtgtg gacggcgata
     1441 actactggat aaccaaggac ggacagaagc gcatgatctc atggtccaat aatgttatgc
     1501 tcgacgaaaa cggcgatgtt gaatatgtaa ttgcgaccgg gatcgatatt accgagcgca
     1561 aggctgccga ggataaatta agactttatc gtgagcttta tctcaattcc aaggatggaa
     1621 tcgttattgt taccaaggag ggcctgtacc aggaatccaa tcctgcttat ctggaacaaa
     1681 ccggatttga ttccgcggat ctcaaaggta tgaaggtgtc cgagctcatg ggcattcccg
     1741 atccggaaaa gtttcagaag gaattattag aaaaaggcgg attccgtgga gagatgattc
     1801 ttcgcaccag ggatggacga gagcttccct tcgatgtttc cgtatttacg attgaggatt
     1861 ccaaggaaga aatagtcaat ctggcaggaa tcggcaggga tatatcggaa cagaaggcgg
     1921 cccaggccgc cctttccaag cgtgtctggt atgaggaggg gctggccgca tgttcccagg
     1981 cgcttctgac aactgcaaac tttgatgatg ctcttcggga agcgctgttt caccttctgg
     2041 tggcatcacg ctccagccga gcctatatct tcgaaaattt tgagagcccg gaggatggtc
     2101 tctgtatgcg ccagacccat gaggtctgcg ataccggaat cgaaccgcag atagagaatc
     2161 aagagcttca acatttccct tacagccggg ggttccagag atggtgcgaa attctgggaa
     2221 gaggggaagc gatctcgggc ccggtggcgc aatttcccaa agccgagcgt gaactcctcg
     2281 agtctcaggg cattcgctcg attatagtgt tcccgattac tgtagaggga agatggtacg
     2341 ggtttgtggg atttgatgaa tgccgccagg aacgagagga taccgaggag gatctgaggc
     2401 tcctcaggac agcttccgag atgatcgggt cgtttgtgga aagcaggaag tttgaggaga
     2461 acctgcgcgt ttcagagcag agattccgca gcctggtcga aaacgccagt gacgttatct
     2521 attccatcag ccccgatggc aaatttacct atatttctcc ccagtttacc gaatccacgg
     2581 gctatgatgt tgccgaatac atcggcaagc cttccacgct ccttttgtca gatgagcaaa
     2641 tcgaggacag caggtcctgg ttcaacgccg gtatgcccga tcatgacgat catgttgagg
     2701 gttatcagtt taagatggaa acaaaaaatg gcgatctccg ctggttcacc gcccataccg
     2761 cgattatcag ggatgatagc gggaatgtaa tcgaggggat aggaattgct cacgatatta
     2821 ccgagataaa aaaacttctc gaaagcctgg aaaaaacgaa ccgggagctg gtgaatactc
     2881 aagcccagct ggtgcagacg gagaagatgg cttcactggg tcaacttgtg gcaggcgtag
     2941 cgcatgaaat taacacgcct atcggcgcgg tcaacagtat gcataactcc ctcataagag
     3001 cggtggagcg attgagtaag atactgagag acaaatatcc cgatgaaatt gcggatggcg
     3061 gagaccttca aaaatatctc catgtcatag ccgaggcgaa ccgcattatt gcctcgggaa
     3121 ctgaaagggt gaccacaata gtgcggcgct tacggagttt tgcacgcctg gatgaagctg
     3181 aactcaagac ctgcgatatt catgaggggc ttgaggatac cctgacactc attcatcacg
     3241 aggtaaaaca tcatatcgaa atcgtaaggg attacgggga cttgccggaa tttgcatgtt
     3301 atccgggcag gatgaaccag gtatttctca atatcctgaa taatgcccgc caggcgatca
     3361 gggacaaggg ccaaatcacc atcaggactt atctgaaaga caacctcgct catatcgaaa
     3421 tcagcgataa cggagtgggc atttccaggc agaatttgaa gaagatattc gatcccggct
     3481 ttaccaccaa aggtgtggga gtgggtaccg gcttgggact ttcaatttgt taccagatca
     3541 taagcggcca ctacggggat atcgaagtcg aaagtgagcc gggaatagga accacattta
     3601 ctatcaaact gccactcaat ctggatgata ttctggaaaa acagagaaat gaaaactgac
     3661 ccatttttct tattcctgtt gattcaggct aaaaatcggg tgtgtggcaa gttgtccccc
     3721 ctttggctgc tgagaggaaa acgcttacag caactgctgc cacatctttc gagtcgccca
     3781 ttcaaacgat tttttgtcat ttatcctgac agataaatat gatacaagat aattcttgtc
     3841 aagtattaaa tttaagatta taattgtttt aacagtgagg gaattaaatt gtataactat
     3901 ttgacattta agaataagtt tgtcaatttg ttacgaaatt gaatgaggaa aaccgaaaga
     3961 tgtttaaatt cctgaactcc ttgcttctaa ttctcacggg catgaccgcg gttatttatt
     4021 ctcaggatat cgaatcgttt tcggctgctc aggcattgtc acggcagtcc ggcaagccga
     4081 tcctgctgga gtttgtgcgc tccgactgag agtactgcca gaaggccgct cgtgaagcgg
     4141 aagaagaaga gatattcgct aatgccctga agcaggtagt gcacctgccg atcaatgttc
     4201 tcgaaggtga cggtccggag ctgaaggaaa gctacgatgt cggcagtaca tatccagttt
     4261 tcattctcac cgacagcgag ggcgagatta taatacgctg gacgggatat tccaccgcca
     4321 gccagttcgt ctacaaatta aatgcctcac tcaaagacct acggactatc aaggagcggc
     4381 gcgctcaatt tgaggctaat ccaacattgc aggaagcgct ggccctggga agattttatg
     4441 ccgaaatcgg tgaacatatt gaggcggttg cgatgtttcg ccgggctgaa aagctcagcg
     4501 cccagaggaa aatcaatctg tccatcgaca tcttcaagaa tatggccgaa gctgtctgga
     4561 aagagatggc gccgtacgat tcaatcttcc cggccgcaga atatgttctg gcttcgggcg
     4621 gcgccccggg gattattaag atggccacgc tgatgagccg tctatcccgc aaatttgata
     4681 ctactgatag cctcaggaaa tatctcgatg ccagcatcca ggccgcgtcc gagagcaaag
     4741 atcctcagca tgctgaggat cgatattcat tggtagcgga gcatgccctg caaattgccc
     4801 acgataccgc caaggcgatt gagatcaagg agctgagcat gggcgctgga tgggaaagca
     4861 atccgaccaa attttatggt tatgccaagt ggtgcctgga gagaaaagtc aaccttggcg
     4921 ccgctgagat gtatgcccgg cgtgcggttc aggtagcggg aagccccgag atgagagcca
     4981 gggtgctgaa cacattagcg gagatctatg aggttcgcgg aaactatgag ggggcgattc
     5041 aggccatcga gatggctatc gagaatcatc ctgaaaatcc atattatcaa accaggctgg
     5101 atgagatcga ggacgccata gcgggcggac agtaagcttt ccctaatcaa tgtcacttat
     5161 acattcagcc ggtaccc
//
LOCUS       DSEB01000031            7178 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000608, whole genome shotgun sequence.
ACCESSION   DSEB01000031 DSEB01000000
VERSION     DSEB01000031.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7178)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7178)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7178
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000608"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..222)
                     /locus_tag="ENO07_00590"
     CDS             complement(<1..222)
                     /locus_tag="ENO07_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDS00490.1"
                     /translation="MEWITDPQIWIALITLTALEIILGIDNIIFISILADKLPKSKQA
                     AARRVGLALAMIMRIGLLVSLKWMMKLTSP"
     gene            complement(1105..3369)
                     /locus_tag="ENO07_00595"
     CDS             complement(1105..3369)
                     /locus_tag="ENO07_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDS00491.1"
                     /translation="MNIEQLAQALKNDNEFMDCVTHWETLPAIDPQYADFPDKLNPRL
                     KEVLIKKGIRQLYTHQREAYDLASEGKNFVVVTPTASGKTLCYNLPVLNRMLSSPDTR
                     ALYLFPTKALSQDQVSELYETILALEADIKTFTFDGDTPQNARRKIREAGHIVVTNPD
                     MLHSGILPHHTRWIKLFENLKYVVIDELHHYRGVFGSHLANVIRRLKRLLRFYNTEVQ
                     FICSSATIANPVELAERILEEKVELIDKNGAPRAEKHFIFYNPPVVNQQLGIRRSALL
                     SAKNIAAKIIANDIQTIVFVPYRTTTEILLTYLRNSLGGHINSKQAVFGYRGGYLPNE
                     RRRIEKGLKSGEIRAVVSTNALELGIDIGQLEAAILTGYPGSIASTLQQAGRAGRKRG
                     LSLVVLVANSSALNQFIVNHAEYIFKQPFESGVVDPNNFVILTSHIKCAAFELPFEEK
                     EKFGVETTTEILRYLAQKGILRETGGKFHWTSDVYPANELSLRSAINDNFVILNSSNN
                     FKVIGEMDLFSTPEMLHPQAIYLHGAAQYQVEELDWEGRKAIVKEVDVDYFTDAESKT
                     DLKVLTVERRDDPSMSGWGEISLTTVTVLFKKVKFFTHENVGSGKVSLPEIEMSTTSF
                     WQEFPLDIYDQIKVDRERMGEVLRGMANCLGDIAPLYILCDRKDIGSISNVKAKFSEK
                     PTLYLYDRVPGGVGFSEKIFTMKKEIYKAARYLIKSCACEAGCPSCIGPPPDNNPIRK
                     NLAVTLFDYLIDLK"
     gene            complement(3438..3980)
                     /locus_tag="ENO07_00600"
     CDS             complement(3438..3980)
                     /locus_tag="ENO07_00600"
                     /inference="COORDINATES: protein motif:HMM:PF07998.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00492.1"
                     /translation="MRIRKSRLVIVPLGEVDYFLINKLATNLSSTLSISANIMQGVKI
                     PPESYNIMRSQYYATVILQKLEIMKSSHKELMLGIMEEDLYAGEEHSVTSEIDRLTGC
                     GVISLMRLRTDFYGLPENEKWVYPRLYKESFKIVARLMGMRACRNPHCILYYSEDMRD
                     IDDKRLKLCDICQREFFKLV"
     gene            complement(4021..5328)
                     /locus_tag="ENO07_00605"
     CDS             complement(4021..5328)
                     /locus_tag="ENO07_00605"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:PRK05820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HDS00493.1"
                     /translation="MLAPDLIAKKRDGEKLSQEEIKFLVENYTADKIPDYQLSAFLMA
                     AFLNGLDTDETFWLTEAMLHSETSIEFPEKLHPVFDKHSTGGVGDKISLTLSPLMASL
                     GYNIAMLSGRGLGHTGGTLDKLESIPGLNPFWSKTQIKNRLGKCGIAISGQTEDIAPA
                     DKKIYALRDLTGRVESIPLITASILSKKLALKTDGIVFDIKSGSGAFMKTAEEARKLA
                     RSLLSVCKKFRHPAVSLLTDMSEPLGHYIGNYMEIIETVNFLKTGEPEDIREVTFRLA
                     YEIARMNGSPENKREFSARLSKAISSADPLQKFIEFVKISGGDTKILSNPESYHRPKS
                     GGRLPAGKSGYIAAFNTELIGKASLVLGAGRLRIEDRIDPMAGIHITKKIGQEIKADE
                     TLFELYGSNRKAVSAAKTMLSESYKIRKEKIASPRKIITLIEN"
     gene            complement(5315..5989)
                     /gene="deoC"
                     /locus_tag="ENO07_00610"
     CDS             complement(5315..5989)
                     /gene="deoC"
                     /locus_tag="ENO07_00610"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDS00494.1"
                     /translation="MLKLESFIDHTLLKPETTSNDIRKLCKEAREYNFRTVCINPSYI
                     ELAVSELKNTDVAVGTVCGFPLGATRTEIKVYEAETAENSGASEIDMVANIGAIKEGA
                     FELVKKDIARVRRALVGSTVLKVILECALLDDEEIESAAKIVADCGADFVKTSTGFFG
                     GATLHHVSLLFNTVGKKTGIKAAGGIRDVKTVLAMIEAGASRIGTSSAVSIMEEYRNR
                     SNNAGS"
     gene            complement(5998..6387)
                     /gene="cdd"
                     /locus_tag="ENO07_00615"
     CDS             complement(5998..6387)
                     /gene="cdd"
                     /locus_tag="ENO07_00615"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDS00495.1"
                     /translation="MISLEDKALEKAKAARENSYSPYSGFRVGAAVISGDDRIFAGTN
                     IENRSYGLTICAERSAIFSAVSKGVRTLRHLVLITDSEKFITPCGACLQVLSEFASDM
                     QIESCNKKGEKQRFSLKELLPKSFKLE"
     gene            complement(6374..7114)
                     /locus_tag="ENO07_00620"
     CDS             complement(6374..7114)
                     /locus_tag="ENO07_00620"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:PRK05362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDS00496.1"
                     /translation="MSAEVYTSADSVFQIAAHTDIVPLEKLYEVCKIARRMLTGEWGV
                     SRVIARPFTGDSGSYVRTSDRKDFSIEPVDETMLDRLINAEHQVIGIGKIEDLFAGRG
                     LTESYHTKNNDYGVDLILKVLARKNNGLIFINLVDFDMLWGHRNDYEGFGKGLEDFDR
                     RLPEILNALRDDDLLMIAADHGCDPTIETSTDHSREYVPLLVYADRFIKRKSLGTRDT
                     FADVAQTVCQNFNLEPMKHGKGFLNDLS"
BASE COUNT         1683 a         1783 c         1824 g         1888 t
ORIGIN      
        1 gggggaggtc aatttcatca tccacttcaa tgacaccagc agacctatgc gcattatcat
       61 ggccagtgcc agccccaccc gtcgcgccgc ggcctgcttg ctcttcggga gtttgtcggc
      121 caggatggaa ataaaaataa tgttgtcgat gccgaggatg atctcaagcg ctgtcagggt
      181 gataagcgct atccagattt gagggtcggt aatccattcc atgagtatgt attttattta
      241 tttttggagg atgggcaata gatatttgag tgcattgttt tgtagcgcag gctcacaatc
      301 ccgagcacaa caataaaagc tcgggttggg gtgccccaat cttcagattg ggttggtggt
      361 ttaaaaaaat atcccaagct aaagggctta ttgataaacc ctgaatacct taatattcgt
      421 catcgcgagg agcgaagcga ttttgaatcc ggctcctgac ggacgtggtg atctccacga
      481 ttattcttga ggcaaccgcg gagattcccg cggtcgtcct cgccttcggc gggactccct
      541 cggaatgacg tgtatggttc gtatttctaa cttgctggct atttcaacac tccctgaagc
      601 ctggggcacc ctgagcgctc atgctgactt ggaacactga ctgttaccga tgtcgtgagt
      661 ctcccttttg aaatcaccat agcaggatga ccgcttaaat cagtcagggg cctgaccttc
      721 ggcccacatt tcatgcgggg taccaagtac cagaaggcgg ggcgcacgag acatacgccg
      781 cgcacgaagt gggggaaacc tattggtcgt gctgcttata ccttcatgtt gtgtgctggc
      841 agccgtcgtg caagaggatg tacagacgca tggatatctg ggtggctatc cgagccggcc
      901 agggaggtcg ttcgagaatg ggtgccgccg acaaacttgt tttgtcggcg atctcttgaa
      961 tcaagcatcc gccacaaacg agtttgtagc gggcacccaa tttcggactg catacacgcc
     1021 caaataaatt tgagcgggcc acccttgcgg cgcatggaga aatacgcctg tccggcattt
     1081 tgcggtccgc gcgcgaatcg tgaattattt caaatcgatc aggtaatcaa aaagggtgac
     1141 tgcgagattt tttcggatgg gattgttgtc ggggggcgga ccgatacatg agggacatcc
     1201 ggcctcacag gcacagctct ttataagata gcgcgccgct ttgtaaatct cttttttcat
     1261 ggtgaagatc ttctccgaaa atccgacacc gccggggaca cgatcataca aatacaaagt
     1321 gggcttctcc gagaacttag ctttgacatt ggagatcgaa ccgatgtctt ttctatcgca
     1381 gagaatatat aaaggcgcaa tatccccaag gcagttggcc atacccctca gcacctcgcc
     1441 catcctctcg cgatcgactt tgatctgatc ataaatatcc agcggaaact cctgccagaa
     1501 agatgtggtc gacatctcga tctcgggaag actgaccttg ccggagccga cattttcatg
     1561 cgtgaagaat ttgaccttct taaaaagcac agtaaccgtg gtaagtgaga tttcgcccca
     1621 gccggacatg ctgggatcat cccgacgttc cacagtcagc accttgaggt cggtcttgga
     1681 ttcagcgtcg gtgaagtaat caacatcgac ctctttgaca atcgccttgc gcccctccca
     1741 atccagctcc tccacctggt actgcgccgc gccatgaagg taaattgcct gcggatgcag
     1801 catctccggt gttgaaaaga ggtccatctc gccgatgacc ttaaaattat tggagctgtt
     1861 caggatgaca aagttgtcgt tgatagccga acgcaatgac agctcgttgg ccgggtaaac
     1921 atcggaggtc cagtggaact ttccgccggt ttcgcgcagg atgcccttct gggccaggta
     1981 gcgtaatatc tcggtcgtgg tttccactcc gaacttttct ttttcttcaa aaggcagttc
     2041 gaaggcggcg cattttatgt gagaagtaag aataacgaag ttattaggat cgaccacacc
     2101 tgattcgaag ggctgcttga agatatattc agcatgattg acgataaact ggttcaaggc
     2161 cgagctgttg gctaccagca ccaccagcga aagcccgcgc ttgcgtccag cacggccggc
     2221 ctgctggaga gtcgaggcga tcgagccggg atagccggtc agaatagccg cctcgagctg
     2281 gcctatatca attccaagtt cgagggcatt ggtcgatact accgcccgta tctcacccga
     2341 tttgaggccc ttttcgatac gtctgcgctc attgggaaga taccccccac gatagccgaa
     2401 gaccgcctgc ttggaattta tatggccgcc caggctgttt ctgagatagg tcaggagtat
     2461 ttccgtggtt gtgcgataag ggacgaagac aatggtctgg atatcattag cgattatttt
     2521 tgcggcgata tttttggccg acagaagcgc cgatctgcgg atgccaagct gctgattgac
     2581 cacgggagga ttatagaaaa taaaatgttt ctcggcgcga ggggcgccgt ttttgtcgat
     2641 cagttcgacc ttctcttcca gtatccgttc agccagttca accggattgg cgattgtagc
     2701 cgaggagcag ataaactgca cctcggtatt ataaaatctc agtaatctct tcaacctgcg
     2761 gatgacattt gccagatgcg agccgaatac tccacgatag tgatgcagtt cgtcgatgac
     2821 cacatacttg agattctcaa acagcttgat ccatctggta tgatgcggca ggattccaga
     2881 atgcagcata tcgggattgg ttaccacaat atgcccggcc tcgcggattt tcctgcgtgc
     2941 attttgaggg gtgtcgccgt caaaggtgaa ggtcttgata tcagcttcca gcgctaaaat
     3001 agtttcgtac aattcgctga cctgatcctg ggacagcgct ttggtgggga agaggtaaag
     3061 cgcgcgcgta tccggactgg acaacatcct gttcagcacc ggcagattat agcagagcgt
     3121 tttgccggag gctgtgggtg ttaccaccac aaagttcttg ccctcggaag caaggtcata
     3181 ggcttcacgc tgatgggtat aaagctggcg tatgcccttt tttatgagca cctctttcag
     3241 ccggggattc agcttatcgg gaaaatcggc atactgagga tcaatagccg ggagagtctc
     3301 ccagtgggtg acacaatcca tgaactcatt gtcattcttc agggcctgag cgagctgttc
     3361 gatattcatg agatttataa tagctgatgt ttccggattt tcaataaaaa catcaaaagc
     3421 aaggcaagaa aatgatttta gaccagttta aaaaattccc tctgacagat atcgcaaagt
     3481 ttgagtcttt tgtcgtcaat atcgcgcata tcctcggaat aataaagtat gcagtgagga
     3541 ttgcggcagg cacgcatgcc catcaagcgg gccacgattt taaagctctc cttgtagagg
     3601 cggggataga cccatttctc gttttcggga agaccgtaaa aatcggtgcg cagacgcatg
     3661 agtgaaatca ccccgcagcc cgtcaggcgg tctatttccg aggttaccga atgctcctcc
     3721 ccggcataga ggtcctcctc cataatgccc agcataagct ccttgtggct ggatttcatg
     3781 atttccagtt tctgcagtat aacggtggca taatactgcg agcgcatgat attatatgat
     3841 tccggcggga tttttacacc ctgcataatg ttggctgaga tggaaagagt tgatgacagg
     3901 ttggtggcca gcttgttgat caggaagtaa tcaacctcgc ccaatggcac aattacaagt
     3961 ctcgatttcc gtattctcat tactcgacaa aaaacctctc tttagcttcg ccctcacctc
     4021 tcaattttct atcaaggtaa tgatttttcg aggcgatgca atcttttcct tcctgatttt
     4081 atacgattca gaaagcattg tctttgctgc ggataccgcc tttctattcg aaccgtatag
     4141 ctcaaagaga gtttcatccg ctttgatttc ctgaccgatc tttttggtta tgtgaatgcc
     4201 tgccattgga tcgattctgt cctcgatccg taaccttcca gcgccaagca caagcgaggc
     4261 tttgccgata agttctgtat tgaatgcggc aatatagccg gatttgccgg caggcagtct
     4321 tccgccggat ttcggcctgt ggtagctttc aggattagac aggattttgg tatcgccgcc
     4381 ggagatctta acaaactcga tgaatttctg cagggggtct gcggagctga ttgccttgct
     4441 aagccgcgca gaaaactccc ttttgttttc cgggctcccg ttcatgcgag cgatttcgta
     4501 ggccagcctg aaggttacct cccttatatc ctcgggctcc ccggttttca ggaaattgac
     4561 cgtttcgata atctccatgt aattgccgat gtaatgcccc aacggctcgg acatatcggt
     4621 cagaagagat acagccggat gcctgaattt tttgcagaca cttaaaagag atcgggccag
     4681 cttcctggcc tcttctgcgg tcttcataaa tgctccggag ccggacttta tgtcaaaaac
     4741 tattccgtcg gttttcagcg ccagcttttt ggaaagtatg ctggcggtga tgagcgggat
     4801 cgattctact cttcctgtaa ggtcacgaag agcatagatt tttttatcgg ccggggcgat
     4861 atcttctgtc tggccggaaa ttgcgattcc gcatttcccc aggcggttct tgatttgtgt
     4921 tttgctccag aagggattca agccggggat cgattccagc ttatccagtg tgccgccggt
     4981 atgacccagg ccccgtccgg agagcatggc gatattatat ccaagagaag ccataagcgg
     5041 ggagagggtc aggctgatct tgtctcccac acccccggtg gagtgtttat cgaacaccgg
     5101 atgcagtttt tcgggaaatt cgatgctggt ttcggaatga agcattgctt cagtcagcca
     5161 gaaagtttcg tccgtgtcga gaccattcaa gaaagcggcc atcaggaacg ctgagagctg
     5221 gtaatcgggg attttatcgg cagtatagtt ctccacaaga aattttatct cttcctgcga
     5281 gagcttttcc ccgtcgcgtt tcttggcgat taaatcagga gccagcattg ttacttctat
     5341 ttcggtattc ttccattata ctcactgccg aggaggttcc gatgcgcgag gccccggctt
     5401 cgatcatggc caggacagtc ttgacatcgc gtatgcctcc ggctgccttt ataccggttt
     5461 ttttgccgac tgtattaaag agcaggctga catgatgaag ggtggccccg ccgaaaaacc
     5521 cggtggaggt cttgacaaaa tctgcgccgc agtcggcgac gattttagcg gcagattcga
     5581 tttcctcatc atcaagaagc gcacattcga ggatgacctt caatacggta cttcctacca
     5641 gggccctgcg gacccgggcg atatcttttt tcaccagctc gaaagcccct tctttgattg
     5701 ccccgatatt ggcgaccata tctatctcgg aagccccgga attttcggct gtctcggctt
     5761 cgtaaacctt tatttcggtc ctggtagccc ccagcggaaa tccgcagaca gtgcccacag
     5821 cgacgtcagt attcttgagc tcgcttaccg caagttcaat ataggacggg ttaatgcaga
     5881 cagtccggaa attgtattcg cgcgcctctt tgcacagctt cctgatgtca ttcgaggtcg
     5941 tttcgggttt taagagagtg tggtctatga atgattccag tttcagcata taaacttcta
     6001 ttctaattta aaggatttgg ggagcagttc ttttaatgaa aaacgctgtt tctcgccctt
     6061 cttgttacag gactcgattt gcatatctga ggcaaattcg gacagaacct gaagacatgc
     6121 cccgcaggga gtgatgaatt tttctgagtc ggtgatgagc accagatgtc tcaaggttct
     6181 gacgcctttt gagactgcgc tgaaaatcgc ggatcgctcg gcgcagattg tcaggccgta
     6241 ggaacggttt tcgatattgg tgccggcaaa aatgcggtcg tccccggaaa taactgcggc
     6301 ccccaccctg aagccggagt atggggaata agagttttcg cgagccgctt ttgctttttc
     6361 aagcgcctta tcttcaagag agatcattta aaaaaccctt tccatgtttc attggttcga
     6421 gattgaaatt ctggcagaca gtctgagcaa catcagcaaa ggtgtccctt gttcccagcg
     6481 atttccgttt gatgaacctg tcagcatata ccagcagggg gacatattcc cggctgtggt
     6541 cggtggaggt ctcgattgtc ggatcgcagc catgatcggc ggcaatcatt aacagatcat
     6601 catcccgtaa ggcattcagg atttcaggga ggcgtctgtc gaaatcttcc agtcccttgc
     6661 caaaaccttc ataatcattg cgatggcccc agagcatatc gaaatcgacc agatttatga
     6721 agatcaggcc attatttttc cgggcaagca ccttcaggat cagatcaacg ccataatcat
     6781 tatttttggt atgatagctt tcggtcaggc cgcgtccggc gaagagatcc tcgattttgc
     6841 cgatcccaat aacctgatgc tccgcattta tcagcctgtc gagcatggtt tcatccaccg
     6901 gctcgatcga gaaatccttg cggtcggaag tcctgacata actgccggag tcgcctgtaa
     6961 acggccgcgc tattactcgt gaaacacccc attcaccggt aagcatcctg cgggcgattt
     7021 tgcagacctc gtagagcttc tcaagcggaa caatatcggt atgtgccgca atttgaaaga
     7081 cactgtcggc ggaggtataa acctccgccg acagtgtctt tcaaattgcg gcacataccg
     7141 atattgttcc gcttgagaag ctctacgagg tctgcaaa
//
LOCUS       DSEB01000032            3740 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002495, whole genome shotgun sequence.
ACCESSION   DSEB01000032 DSEB01000000
VERSION     DSEB01000032.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002495"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            64..2412
                     /locus_tag="ENO07_00625"
     CDS             64..2412
                     /locus_tag="ENO07_00625"
                     /inference="COORDINATES: protein motif:HMM:PF06452.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00497.1"
                     /translation="MWKYWGHCWRGNLYMERNIFKTGRRAVSPGGARFLVVISIALMI
                     LLSLSALLTAESYKLYVTHPNYYEDGRGGISIVNYANKEVIAPLLTTPSADKAVFVEK
                     LNRLFVFSSERGVCEVFDPVIDRRITEFTTGGPVADVIFSLNGRRMYVANGSDSPDPK
                     NTVTIIDTETGDQIFEIVAGRNPTALELSADGSLLYIADGQSGVVNVVELTNFQVLRS
                     FYAGVSPSDLELSWDGRSLLVCSRSVSSANDAGAGIAVIDLGHENVAELVGTDGDIEK
                     LALTENRVVVVETSEGRSNLILYDYLEDNGRIMLSRAGRIISAEEILDFQLNRSGNEA
                     IASFANGTVKVYDLGTFSEKAKISELTNGFSGGLVLVPIDFARALARRDSLIASDPAS
                     DEARTAYFEKAYLYRSMCDKNSELKIYTELAEEFPRTETEVLSLLRLGDLCYSDMLYA
                     NSAEFYSRAFDAYTGVLEQNAGVADIDQSLLMTAVRRLGEFSAEYDKDYLKSIMSRLE
                     TLSVSSGELAELFFEIAYYLKKQGDTRLARRSLDEVERQMINIQDKNLYKRLRNKIDL
                     LESKGRVVLIADKVKNGPLIDGDMADWEDKHTLFVDRRTDVLVNSERWVDDRDLSVEL
                     RAAYDRESLYLLGVVTDDSVFSSDIEKQDKFILYFDMRENSGDFLQRKSEASQGLARL
                     EIIPAVSPEGRFDISHSPEIQPIFAGKLTEKGYVVELKIPFAYFKNIGPDNEKSFGFG
                     FEIMDADSDLTRDPVKVMGWVAPAESLNGSRDYRMLGILDFD"
     gene            2828..>3740
                     /locus_tag="ENO07_00630"
     CDS             2828..>3740
                     /locus_tag="ENO07_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDS00498.1"
                     /translation="MAILKIDSVTKVFDRKVAVKNFSLEVQEGVIYGLLGPNGAGKTT
                     TIRMVMNIIAPDEGRITVMGREMNEQAKDRIGFLPEERGLYQKMVVRDVLTYLGEIKG
                     MKKPQIAGAIEEWLARVKLSECIDDKVEELSKGMQQKLQFASTLIHDPQLIMLDEPFS
                     GLDPVNLELIKNIVLEQKRQGKTIIFSTHIMEQAEKLCDNICLINKGEKVLDGPLFEV
                     KSKFGKNTVVLEFDGDISDLEGLPGVEKIYDYNKYVELKLHDGTSPHKVLESLIDKVE
                     LNRYEFMEPSLHDIFVDIVGEKKDAREE"
BASE COUNT         1013 a          782 c         1003 g          942 t
ORIGIN      
        1 attttgccga tttagttata ttagataaag atttacattg tataagcccc gatcaaatct
       61 cgcatgtgga agtattgggg acactgttgg agggggaatt tgtatatgga aaggaatatt
      121 ttcaagactg gtagaagagc ggtgagtcct gggggagcca gattccttgt tgtcatatcc
      181 atagccctga tgatattact ctctttgagc gcgctgctga cggcggaaag ctacaagctt
      241 tatgtgactc atcccaatta ttacgaagat ggacggggag gaatttcaat agtaaattat
      301 gcgaataaag aggtcattgc tcctctcctg acgaccccat cagcagataa agccgtattt
      361 gtggaaaaat tgaaccggct gtttgtcttc tcgtccgagc ggggagtctg cgaagtattt
      421 gaccctgtca tcgacaggag aataacagaa ttcacgaccg ggggcccggt ggcggatgtt
      481 atcttctcgc tgaatggaag aaggatgtat gtggccaatg gcagcgacag ccccgacccg
      541 aagaatacgg ttaccatcat tgataccgag accggagacc agatttttga aatagtagcg
      601 gggcggaatc cgaccgccct ggagctttct gcggatggca gcctgctgta tattgcagat
      661 ggccagagcg gagtcgttaa tgttgtcgag ctgacgaatt tccaggtctt gagatcgttt
      721 tatgccgggg tctcgccctc cgatctggaa ctgtcctggg atggacgcag tcttctggtc
      781 tgctcgcgaa gcgtatcctc tgcaaacgat gccggcgcag gtattgcggt aatagatctg
      841 ggtcatgaaa acgtagcgga actcgtcgga accgacggag atatcgagaa actggcgctt
      901 accgaaaaca gggtggtggt tgtcgaaacc agcgaggggc gttcgaacct gatcctctat
      961 gattacctcg aagataacgg cagaataatg ctgtccaggg cgggcagaat tataagcgca
     1021 gaggaaattc tggattttca gttgaaccgg agcggtaatg aagccattgc atcattcgct
     1081 aacggcaccg tgaaggtata tgatctgggc acattttccg aaaaggccaa gatttcagag
     1141 ttgaccaatg gattttccgg cggtctggta ctggtgccga tagatttcgc aagagccctg
     1201 gcccggcgcg attccctgat agcatccgat cctgccagcg atgaagcgcg tacagcttat
     1261 tttgaaaagg cctatcttta ccgctcaatg tgtgataaaa actcggagtt aaagatatac
     1321 accgaactgg cggaagaatt tccgcggacc gaaacagagg ttctctctct cttgcgactt
     1381 ggcgatttat gttacagcga tatgctctat gccaacagcg cagaattcta ttcccgggca
     1441 ttcgatgcct ataccggagt gcttgaacag aatgcgggag ttgctgatat cgatcaaagc
     1501 ctgctgatga ctgctgtacg aaggctgggt gaattttcgg ccgaatatga taaggattat
     1561 ctcaagagca tcatgtccag gctggagacc ctttcggttt cctcgggcga actggcagaa
     1621 ctcttttttg aaattgccta ctatcttaag aagcagggtg atacgcgcct ggcacgacgc
     1681 agtctcgatg aagtggagcg ccagatgatc aatatccagg acaaaaacct ttataaaaga
     1741 ctgcgcaata aaattgacct cctggaaagt aagggacggg tagttctgat tgcggacaaa
     1801 gtcaaaaacg ggccgttgat tgatggagat atggcggatt gggaggacaa gcacacgctt
     1861 tttgtcgacc gccggacgga tgttctggtc aacagtgagc gatgggtgga cgaccgtgac
     1921 ctgtcagtgg aactgcgggc cgcttacgac cgggaaagtc tctatctgct gggagtggtg
     1981 actgatgatt cagttttttc ctcggatatt gaaaaacagg acaaattcat tttatacttt
     2041 gatatgaggg aaaattcagg ggacttcctc cagcgcaaga gcgaggccag tcagggcctg
     2101 gccagacttg agataatccc cgccgtctcc ccggagggaa gattcgacat cagccattca
     2161 cccgaaatcc agcctatttt cgcgggcaag ctgactgaaa aaggatatgt tgtggagttg
     2221 aagataccat tcgcctattt taaaaacata ggtcctgaca acgaaaaatc tttcggcttc
     2281 ggcttcgaga tcatggatgc tgacagtgac ctgacaaggg atccggtgaa ggtaatggga
     2341 tgggttgctc ctgctgaatc cctgaatggc agtcgtgatt accgcatgct gggcatactt
     2401 gatttcgatt agaatcgtct ctggccgttc gaagagatcg tcccgattca gataagtata
     2461 gtccccctgc cgacccggtc acagttttgg cccgagagtc ggtaactgcc tgcagccgaa
     2521 cgggatagtt ttagattaaa ttccgatgag ataagaatcc tattgcagga attcactcct
     2581 ttcgcttact gtttcttgat acctgctccc aaaaattctc tctcgcatcc agatattatt
     2641 cccgccagat tgaaacaatc ggctctaaat caagtattgt gaaaagaaat cccgcaggaa
     2701 aaataagaag cccgtgaatg gaattcgtat ttatttattg aattaaatca aatgatgaga
     2761 ttattattat tatactagtc aattatcgtc gaggaaaaga atattgaaga tagatcggga
     2821 gcggcaaatg gctatattga aaattgattc tgtgacgaaa gtctttgacc ggaaagtagc
     2881 cgtaaagaat ttctccctgg aggtgcagga aggagttatc tatggtcttc tggggccgaa
     2941 cggcgccggc aaaactacca ccattcgtat ggtgatgaat atcattgcgc ccgatgaagg
     3001 caggataact gttatgggca gggaaatgaa tgagcaggcc aaagacagaa ttggatttct
     3061 tccggaagag cgaggcctgt atcagaaaat ggttgtgcgt gatgtgctca cctacctggg
     3121 tgaaattaaa gggatgaaaa aaccgcagat cgccggagcc atcgaagaat ggcttgcgcg
     3181 tgtaaagctt tcggaatgca tcgacgacaa ggtcgaggag ctttccaagg gtatgcagca
     3241 gaagcttcaa ttcgcttcca cacttattca tgatccacaa ctgattatgc tggatgagcc
     3301 tttttccggc ctggatcctg tgaatctgga actgatcaag aatattgtcc tggagcaaaa
     3361 acgccagggc aagacgatta tattctcaac tcacattatg gagcaggcgg agaaattgtg
     3421 tgacaatatc tgtcttatca ataagggtga gaaggtcctg gatggccccc tgttcgaagt
     3481 caagagcaaa ttcggtaaga atacggtcgt gcttgaattt gacggcgata tctccgatct
     3541 ggaaggttta ccgggagtgg agaaaatata tgattataat aaatatgttg aattgaagct
     3601 gcatgacggc acctctcctc acaaggtgct cgaatcgtta attgataagg tcgagttaaa
     3661 ccgctacgag tttatggagc cctctttgca tgatatcttt gtcgatatag tgggggagaa
     3721 gaaggatgca agggaggagg
//
LOCUS       DSEB01000033            7199 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000605, whole genome shotgun sequence.
ACCESSION   DSEB01000033 DSEB01000000
VERSION     DSEB01000033.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7199)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7199)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7199
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000605"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1273
                     /locus_tag="ENO07_00635"
     CDS             <1..1273
                     /locus_tag="ENO07_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDS00499.1"
                     /translation="VDNEFKSWRNTDFAHRSKLMNKAAEILKKNKDDYARTMAMEMGK
                     PVAEARAEVEKCAWVCEYYAENAEKFLADMEIETDASRSFVAFQPLGTVLAIMPWNFP
                     FWQVFRFAAPALMAGNTGVLKHASNVPGSALKIEEVFQEAGFPENAFRTLLIGSSKVE
                     AVIKDERVKAVTLTGSEPAGMKVAAVSGSVLKKTVLELGGSDPYIVLEDADMPACVQT
                     AVKARMINNGQSCIAAKRFIVVKSKLKEFEEAQTNIMKNLKVGDPLDEDTQAGPMARQ
                     DLLDELHEQVMQSVEKGAKVLTGGEPMDSEGAFYPPTILTNCKRGMKVVDEETFGPVS
                     AIIPVKDAEEAITVANDSDLGLGGAVWSQDVEKAIEVARRVETGNMFVNGMTKSDPRL
                     PFGGVKKSGYGRELSVFGIREFVNIKTVWVG"
     gene            1590..2069
                     /locus_tag="ENO07_00640"
     CDS             1590..2069
                     /locus_tag="ENO07_00640"
                     /inference="COORDINATES: protein motif:HMM:PF01797.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00500.1"
                     /translation="MSLRYPEQRNFSCFFVTTTFSRWRKFGNIKAVYEKLADSLVFCC
                     NKYQANISGYVFMPSHLHLLLFIEGSDLGNFMRDFKKYIAQKAFRDLDIVSKDIWNEG
                     YDRIGVVSRKVFITKLEYIHNNPVKSGLVRSHEEWRWSSAVDYYTKNAGVIKVYKDW"
     gene            complement(2260..3387)
                     /locus_tag="ENO07_00645"
     CDS             complement(2260..3387)
                     /locus_tag="ENO07_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00501.1"
                     /translation="MRKNFKTILTALVALSFLILAFIIGSCCDDCPVGPVQPKPYNGW
                     LYAIDMQNRWVYKIDVENDSLVDSVYYETTRRSVPGTIDVSTDGRFLAVGYYDLVNGV
                     RFTRIYDAQTLDTLTDLADYNIPVFIPDQDIMIGIGQRLRIYSLPDFTIVHEEATSLS
                     AYPIVNIKNDLVFMNGAVNPEHVESTFIYSYDYVNREIEREWYFRDKHDSIVFVTAFD
                     INKDGTKIYCLNYSNLDGTMVTCYDLETEEIIFSYPIFSWTGSVRLTPDEEYLYVTEP
                     GDLAYYGNPGTVYILDANDGSYIEGISLFGYAPNPFHPLYATPIVFTPQGHKAYIGSG
                     KVVLESGTISVIDTESRDVIENIWPDLGHFISDLKIGPKIK"
     gene            complement(3403..4575)
                     /locus_tag="ENO07_00650"
     CDS             complement(3403..4575)
                     /locus_tag="ENO07_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00502.1"
                     /translation="MRKNFKTILTTLVALSLLILTFSIYSCCEDCPVGPVQPKPYNGW
                     VYATDILNDYLFKIDTETDSVVDSLKCEKGENYMPGRVVCSDDGRYIAISFGNELIEQ
                     YLLRIYDAQTLTLISEVEEPINPLFFLENGSLLLTRVGKEFVYYSVPGLGVTDRDSMI
                     YSHPNLFRLDMLGIDESQNLAYIIARFDREGAPLYSDSVCLYAYDYVNKEIDQEWVIN
                     IRDGNGDFYIVNEAALDVGRSHIYAICGSYADAATFVVGYDYRDDQILFENRIDGAAG
                     DIELTPDEHEIYVVDPGQPGDIWTAGTLYVFDAHTGAYTHGISLYGFVPNRPDIPMYA
                     NVIEFTPTGDKAYIGSGLHDKWNGNVCSINTSTYAVENLAWPDFNHIILRLATGPK"
     gene            4948..5160
                     /locus_tag="ENO07_00655"
     CDS             4948..5160
                     /locus_tag="ENO07_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00503.1"
                     /translation="MESSNLLVVCALAFGAVFLLLLFLAIVMRVILAVFPQRAGASDA
                     AVYAAVTVAASQLYPGTIIKKIEEIK"
     gene            5157..7040
                     /locus_tag="ENO07_00660"
     CDS             5157..7040
                     /locus_tag="ENO07_00660"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00364.20,HMM:PF00682.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin attachment protein"
                     /protein_id="HDS00504.1"
                     /translation="MIFAEKPKKIRVMFTPFRDGLQSSFGGKVRLHDFLPAMEESAKA
                     GIKHFEFGGGARYQAPYFYLGEDPFIDMQKMREAVGPECDLQILTRSVSGVTLTTQSI
                     EGLRLQAKLMKKYGTTWDRNFDYMNDIRNLVNTGRPIVDAGMHHQCCIALMGLPFKSD
                     KVHTPQFYFDIGKKLLDSDVQIDSLCLKDASGTTDPKTIYETAIRLKKIMPPEMPLWL
                     HTHDTGSLAVACYMAGIAAGVDGIDLSCRPMASGTVQPDVRSMAHSLKGTGYSLDVDE
                     TKMPKIESMLSELMKDYDFNPTTTMADARVLGFPMPGGAIGPNVHMMAKAGIIDKYSQ
                     VLEEFPVVVEAGGAWTSVTPGSQQYWLQAFNNVLYGRWKKIDAGYGKAVLGYFGKTPL
                     PPDPKVVKIAEEQLELPPIDGDPLEAAPKNLKVAREALEERNLEVSDHNIFLVLAAMV
                     PGKKMELNEGIRLLTGRPKIDIPLKTKVDEDEQMAKKEEIRSRLAQQAGAVPVPTGPL
                     TTQCTVEENGKRRVFRITMEPATSAAGGGTAAATAAEPSEPAPVESNGTPIYSSFAGS
                     VEVVDIMVKVGDQVTKGQVVAAVEAMKAKHDIKSPIAGKVTSISAEIGDELDSSKPIM
                     TIS"
     gene            7051..>7199
                     /locus_tag="ENO07_00665"
     CDS             7051..>7199
                     /locus_tag="ENO07_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaconyl-CoA decarboxylase subunit beta"
                     /protein_id="HDS00505.1"
                     /translation="MDTIIELLRQSGFAHLSWGNWVMYAVAGILIYLAIAKQYEPLLL
                     IPIGFG"
BASE COUNT         1936 a         1687 c         1789 g         1787 t
ORIGIN      
        1 agttgataat gaattcaaga gctggcgcaa cactgatttt gctcacagaa gcaaactcat
       61 gaacaaagcg gccgagattt tgaagaagaa taaagatgac tatgcccgca cgatggctat
      121 ggagatgggt aagcctgttg ccgaagccag ggcggaggtt gaaaaatgcg cctgggtctg
      181 cgagtactat gctgaaaatg ccgaaaaatt tctggccgat atggaaatcg agaccgacgc
      241 cagcaggagt tttgtcgcct ttcagccgtt gggtacggtg ctggcgatta tgccctggaa
      301 tttccccttc tggcaggtct tcaggtttgc agccccggct ttgatggccg gcaataccgg
      361 tgtattgaag catgcttcca atgtccccgg gtcagccttg aaaatcgaag aggtttttca
      421 ggaggcggga tttcctgaga acgcctttag aactttgctt ataggctcaa gcaaagttga
      481 agccgtgata aaagacgaac gggttaaagc ggtaacgctg acaggctcgg aaccagcggg
      541 catgaaggta gctgccgttt cgggaagtgt tttgaagaaa acggtgctgg agctgggagg
      601 atcggatcct tatatcgtgc ttgaggatgc ggatatgccc gcatgcgtgc aaactgcggt
      661 caaagcccgc atgatcaata acggccagag ctgtattgcc gccaagcgtt ttattgtggt
      721 caaatccaag ctcaaggaat ttgaggaagc ccagaccaat atcatgaaga atctgaaggt
      781 gggcgatcct ctggacgagg acactcaggc cggtccgatg gcccgccagg atctgctgga
      841 tgagctccat gagcaggtca tgcagtcggt tgagaaaggc gccaaggttt tgaccggcgg
      901 ggaaccgatg gatagtgagg gcgcttttta tccccccacc atcctgacaa actgtaagag
      961 gggaatgaaa gttgtggatg aggagacatt cggcccggta tcggcgataa ttccggtcaa
     1021 ggatgccgag gaggcgatta cggttgccaa cgattccgat ctgggcctgg gcggcgcggt
     1081 atggtcgcag gatgtcgaga aagccattga agttgcgcgc agggtggaga ccggcaatat
     1141 gtttgtaaat ggcatgacca aatccgaccc gcgtctcccc ttcggcggtg tcaagaaatc
     1201 cggctatggc cgtgaacttt ctgtctttgg aatcagggaa ttcgtgaata taaagaccgt
     1261 ctgggtgggg taatcaattt agaactttgt ttgcgaaagc ctctgctatc cggcaggggc
     1321 tttttgattc atggcgcagg ggcacatccg gccaaagccg gacaagaccc cgcgcgacgg
     1381 aagtatttat gggtgcccca tctgaagcgt agcgacaggt gggacgtcac gagaacccgc
     1441 gtgcaatatt tgtatcatcc atcaacctgc tatgcccgaa tattatggct gaatgcgata
     1501 tggagtagtg ccgggtcttg atccgccttg gcggattgtg acccggcact ttttatgtat
     1561 caaaattaac ttccgatcat ctataaaatg tgagtcttcg atatcccgaa cagaggaatt
     1621 tcagttgttt cttcgttacc accactttca gcaggtggag aaagttcggt aatatcaaag
     1681 ccgtttatga gaaactggct gattcgctgg ttttctgctg caataagtat caggcaaata
     1741 tatcaggtta tgttttcatg ccctcgcatc tgcatttact cctgtttatt gagggaagtg
     1801 atttgggaaa tttcatgcgc gacttcaaaa agtatattgc ccaaaaagct tttcgtgatc
     1861 tggatatagt atcaaaggat atctggaacg aaggatatga tcgaattggg gtagtctcca
     1921 ggaaagtatt tatcactaag cttgagtata tacataacaa tcccgtcaaa tcgggtttgg
     1981 taagaagcca tgaggagtgg cgatggtcaa gtgcggtgga ttattatact aagaatgcgg
     2041 gtgtaattaa agtctataaa gactggtgat tcatggcgca gggtcacatc cggccagggc
     2101 cggacaagac cccgcgcgac ggataaatta tttccaaagt atactgatat ccctcggaat
     2161 cctcataagt atagcttttg gcaattatag ccgggcaaaa aattgcaaat ccaattagga
     2221 aatgtattac aaggcaatac tttttcataa tctatccttt catttgattt taggccctat
     2281 tttcaaatct gaaatgaaat gcccgagatc gggccagata ttttcgataa catctctgga
     2341 ttcagtatcg attacagata ttgtgccgga ttccaagact actttgccgg atccaatata
     2401 ggccttatgt ccctgtggcg tgaaaacaat gggcgtggca tagagaggat gaaatggatt
     2461 gggagcatac ccaaatagcg atattccttc aatataactc ccatcattcg catccagaat
     2521 atacacagtg ccgggatttc cataatacgc taaatccccc ggttctgtaa catatagata
     2581 ttcctcatcc ggtgttagcc ggacacttcc tgtccaggaa aatataggat aagaaaagat
     2641 aatctcttct gtctcaagat cataacaggt taccatggtc ccatcgagat ttgaataatt
     2701 gagacagtat attttggttc catccttgtt tatatcaaat gcagttacaa atactatgga
     2761 atcatgcttg tcgcggaaat accattctcg ttctatttcg cgatttacat aatcatatga
     2821 ataaatgaaa gtggattcga catgctcagg attgacggca ccattcataa acacaaggtc
     2881 gtttttaata tttacgatcg gataagcgga aaggctggta gcttcttcat gaacgatcgt
     2941 gaaatctgga agggaataga tccttaatct ttggcctatt ccgatcatga tatcttgatc
     3001 cggtatgaac acgggaatat tatagtcagc cagatcggtg agagtatcga gcgtctgcgc
     3061 atcataaatt cgagtgaagc gtacaccatt aaccagatca taatagccga ccgcaagaaa
     3121 gcgaccatca gtagatacat ctattgtccc agggacagat ctcctagttg tctcataata
     3181 cacgctatca accagtgaat cgttttccac atcaatttta tacacccatc tgttttgcat
     3241 atcaatcgca tacagccagc cattgtacgg tttaggctgg accgggccga ccgggcagtc
     3301 atcacagcag gaaccgatta taaacgccaa gatcagaaaa ctcaaggcca ccaaagctgt
     3361 cagtattgtt ttgaagttct ttcgcaaaat acctcctttc tattattttg gcccggtggc
     3421 aagacgcagt atgatgtgat taaagtcggg ccaggctaag ttctcgaccg cataggtcga
     3481 ggtgttaatt gaacatacat tgccattcca tttgtcatgc agccctgagc cgatataagc
     3541 cttatctccg gtcggcgtga actcgatcac attggcatac atgggtatat cgggacgatt
     3601 cggaacaaaa ccatacagag aaatcccatg agtatatgcc ccggtatgag catcaaaaac
     3661 atacaacgta cccgctgtcc agatatctcc aggctgtccc ggatcgacaa cgtagatctc
     3721 gtgctcgtct ggtgtaagtt ctatgtctcc tgctgcgcca tctatgcggt tttcaaaaag
     3781 aatctgatca tccctgtagt catatcctac gacaaatgtt gccgcgtctg cataggaacc
     3841 gcagatggca taaatatgtg atctccccac atcaagagcc gcctcgttga caatgtaaaa
     3901 atccccgtta ccatcgcgta tattgataac ccattcctga tcaatctctt tattcacata
     3961 gtcataagca tatagacaga cagaatcgct gtataaaggg gcgccttctc gatcaaaccg
     4021 ggcaattata taggccagat tctgcgactc gtctatcccc agcatgtcca gccgaaagag
     4081 attcggatgg gaatagatca tgctgtcgcg gtctgttacg cccaggccgg gtacactgta
     4141 gtacacgaat tccttgccga ccctggtcaa gagaagactg ccgttttcaa ggaaaaagag
     4201 cgggtttatc ggttcctcca cctctgatat caatgtcaat gtctgagcat cataaattct
     4261 cagcagatac tgctcaatga gttcattccc gaagctgata gcaatgtatc tgccgtcatc
     4321 cgaacagacc actcgtcccg gcatataatt ctcacccttt tcacacttca ggctgtccac
     4381 cacggagtct gtctcggtgt ctatcttgaa aaggtaatca ttcaggatat ccgtagcata
     4441 cacccagcca ttgtacggtt taggttgtac cgggccgacc gggcagtcct cgcagcagga
     4501 ataaatggaa aatgtcagga ttaataaact cagggccacc aaagttgtca gtattgtctt
     4561 gaaatttttc cgcaaaatac ctcctttcaa aacaagacat aagtgtcaat ttagatgata
     4621 aaatgctcac gtccatgtat tgtgcttcat acttcctcct tttccgcagg ttaaggccta
     4681 acctgctgga tctgaagtta tcttataaaa acttagatgc gccaatgggt gctatttcaa
     4741 cttcaacaat aatctactca tattaccatg acgctgtcaa gccaaaatcg ttcagactct
     4801 ctatgatatt gcaatttcgc taaaaaagtt gacattctta ccaaaaagca tatattttta
     4861 tcttctgcat gaggcgagaa acacaaacat accaacttag cgggctgaca atacctgccc
     4921 ggcgagaatt accaagagga tttacatatg gaatcatcta atctgctggt tgtatgtgcc
     4981 cttgcattcg gcgcagtatt cctgctgcta ttatttttgg ccattgtaat gcgagtgatc
     5041 ctggcagtct ttccgcagag agcgggagcc tcagatgccg cggtctatgc ggctgtcaca
     5101 gtagcagcat cacaattata tccgggaaca atcattaaga agatcgagga aataaaatga
     5161 tttttgcaga gaaacccaaa aaaatcaggg taatgttcac gcccttccgc gacggccttc
     5221 agagctcatt cggcggaaaa gtaagactcc atgatttcct tcccgccatg gaagaatcag
     5281 caaaagccgg aatcaagcat tttgaattcg gcggcggcgc aagataccag gcgccctatt
     5341 tttacctggg tgaggatccc tttatcgata tgcagaagat gcgcgaggcg gtcggtccgg
     5401 aatgcgatct gcaaatcctg accagatcgg tctcgggtgt tacgctcacc acccaatcga
     5461 tagaaggttt gaggctgcag gccaagctga tgaagaagta tggaacaacc tgggaccgca
     5521 acttcgatta catgaacgac atcaggaatc tggttaatac cggaagaccc attgtcgatg
     5581 caggcatgca tcatcagtgc tgtatcgccc tgatgggcct gccttttaaa tcggacaaag
     5641 tacatacacc gcagttctat ttcgatatcg gcaagaaact gctggatagc gatgttcaga
     5701 tcgacagtct ctgtctgaag gacgccagcg gtaccaccga tcccaagaca atttatgaga
     5761 cggctattcg attgaagaag attatgccgc cggagatgcc gctctggctg catacgcacg
     5821 ataccggcag tctggctgtg gcctgctaca tggccggtat tgcggccggt gttgacggta
     5881 tcgatctgtc gtgtcggccc atggccagcg gcactgtcca gccggatgtg cgctcgatgg
     5941 cgcattctct caagggaacc ggctactctc tggatgttga tgagaccaag atgcccaaaa
     6001 tcgagagtat gctcagtgaa ctgatgaaag attatgattt caatcctacc acaaccatgg
     6061 ccgacgctcg tgtgctgggc ttccccatgc ccggcggagc tatcgggccc aatgtgcaca
     6121 tgatggctaa ggcgggaatt atcgacaaat actcgcaggt cctggaggaa tttccggttg
     6181 tagttgaggc cggaggagca tggacctcgg tcactccggg cagccagcaa tactggctgc
     6241 aggcttttaa taacgttctt tatgggcgct ggaaaaagat tgatgcggga tacggcaaag
     6301 cggttcttgg atattttgga aagaccccgc ttcctcctga tccgaaggtg gtcaaaatag
     6361 ccgaggaaca acttgagctt cctcctatcg acggcgatcc gctcgaagcc gcccccaaaa
     6421 atctcaaggt ggccagggag gcgctggagg agcgaaatct cgaagtcagc gatcataaca
     6481 tattcctggt gctggcagct atggtgccgg gcaagaagat ggagctgaac gagggcattc
     6541 ggctcctaac cggcaggccc aagattgaca ttcccctcaa gaccaaggta gatgaagacg
     6601 aacagatggc caaaaaggaa gaaattcgca gccgtctggc ccagcaggct ggcgccgtcc
     6661 ccgttcccac gggtcccctg accacgcagt gcactgtgga agagaacggc aaacgcaggg
     6721 tattccggat caccatggaa cccgccactt ctgccgcagg tggtggaact gccgctgcca
     6781 cggccgctga accttctgag ccggcacctg tggaatcaaa tggcacaccg atatactcat
     6841 cctttgcagg ctcggtagag gttgtcgata tcatggtgaa ggtcggcgat caggtgacaa
     6901 aaggccaggt ggttgcggcg gtcgaggcga tgaaagccaa acatgacata aaatcgccta
     6961 tagctggaaa agtaaccagt atcagtgctg agattggaga cgagctggat tcatccaaac
     7021 cgatcatgac aatttcataa aggcggcgcg atggacacta tcatagaact gctgcgtcaa
     7081 tcgggattcg cccacctgtc ctggggtaac tgggtcatgt acgcggtggc cgggattttg
     7141 atctacctgg ctatcgctaa acaatacgaa ccattgctcc tgatccccat cggcttcgg
//
LOCUS       DSEB01000034            7216 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000602, whole genome shotgun sequence.
ACCESSION   DSEB01000034 DSEB01000000
VERSION     DSEB01000034.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7216)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7216)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7216
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000602"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..820)
                     /locus_tag="ENO07_00670"
     CDS             complement(<1..820)
                     /locus_tag="ENO07_00670"
                     /inference="COORDINATES: protein motif:HMM:PF01257.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010872722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HDS00506.1"
                     /translation="MECEMKIDSPDKLNAWKEECLQKDAKLKKKVMVCFGPGCLAAGC
                     REIYAKFEEILAERKLGDITLESFKKTGCHGLCEKGPLVVIEPEGIFYNRVRTSQVKD
                     IVEKTLAKGEIIERLLYKNPKTKERVLKYHDIDFYKKQTRLALRNLGHIDPDNIEDYV
                     MRDGYQSVAKVLTGMTPEEVIDEVKLSGIRGRGGGGFPAGRKWATCRGVESDVRYVIC
                     NGDEGDPGAFMDRSIMEGDPHSVLEGMIVASYAVGARDAYIYVREEYPLAVQHLY"
     gene            complement(798..1271)
                     /gene="nuoE"
                     /locus_tag="ENO07_00675"
     CDS             complement(798..1271)
                     /gene="nuoE"
                     /locus_tag="ENO07_00675"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HDS00507.1"
                     /translation="MKPEIDVAELKEIISHSPQKPSSLIAVLQDIQRKFHYLPPEAIK
                     ETSSALGVPLSQVYSVATFYSAFSLEPRGKHTCRICMGTACHIKGAPMALEQAEDQLG
                     IKAGQTTPDLRFTLEVVNCVGACAMAPVVIIDEKYYGNVRVDKMKRYLDGVRDED"
     gene            complement(1268..1831)
                     /locus_tag="ENO07_00680"
     CDS             complement(1268..1831)
                     /locus_tag="ENO07_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     gamma"
                     /protein_id="HDS00508.1"
                     /translation="MSQYEVTFAGFGGQGIMTAGQLLAYTGMNAGKQVAWIPSYGPEM
                     RGGTAYCTVVVSDERIGSPIINRPQGICVFNRPSLDKFGPRVKPGGLLIINSSLVDAT
                     SDRDDIQVLEIPANDIAQKAGSGKATNMVVLGAFVGATGIVEFDKLKETLTEKMGRKK
                     DLLQINYKALDEGYELGRKAKEEKVKG"
     gene            complement(1828..2604)
                     /locus_tag="ENO07_00685"
     CDS             complement(1828..2604)
                     /locus_tag="ENO07_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDS00509.1"
                     /translation="MPEAKLKIFERPKSFTDVVTHYCPGCTHGIIHRLIAETIDEYGI
                     RGKTVGVAPVGCSVLIYNYFRTDFLEAPHGRAPALATGIKRVSPELIVFTYQGDGDLA
                     SIGMGEIVHAANRGEKITVVFVNNAIYGMTGGQMAPTTMPGQVTTTSPMGRDVSQAGY
                     PIRMSELLSTLRTPAYIERVSVHNPHNVIRAKKAIKKAFKNQMEGKRFTFVEVLSTCP
                     TNWGLTPDEAQNWLEDNMIAFYPLGVFKDTYYDNDEEGQK"
     gene            complement(2618..3688)
                     /gene="vorB"
                     /locus_tag="ENO07_00690"
     CDS             complement(2618..3688)
                     /gene="vorB"
                     /locus_tag="ENO07_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDS00510.1"
                     /translation="MAKILMKGNEAIGEAAVRAGGLHYFAYPITPQSEVGEYLVKRLP
                     EVGGVFVQAESEVAVGNMLFGAAASGKRVFTTSSSPGISLMQEAISYIAGAHLPAVFI
                     NIVRGGPGLGGILPAQSDYFQATKGGGHGDYRLLVLAPSTVQEAVDLVMLAFDLAHKY
                     LNPVMVLGDGMIGQMMEAVEFPESIKETDDPMTMDWPATGCEGRDPRIIKSLYLDPQA
                     LEKNSFALADKYKKMKEEEVRYELYKVKDDCQVLLISYGTMARICQTVIDEMEEEGVS
                     VGLLRPITLFPFPENEIREQAWKENIRAVLSVEMSTGQMVEDVDKAVQGRKPVEFYGR
                     TGGIVPSPEEVKDYILKILGKK"
     gene            complement(3707..3940)
                     /locus_tag="ENO07_00695"
     CDS             complement(3707..3940)
                     /locus_tag="ENO07_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDS00511.1"
                     /translation="MPGITIDKNYCKGCELCVKACPQQIISMSKEITKRGYFYAMVHD
                     PSRCIGCRLCAITCPDAAIEVHSHGTRYVLFEY"
     gene            complement(3970..4701)
                     /locus_tag="ENO07_00700"
     CDS             complement(3970..4701)
                     /locus_tag="ENO07_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiA"
                     /protein_id="HDS00512.1"
                     /translation="MSKALEMAEHKLSSLEKRILIFAGGYGSGKTEVAVNYAAYLARL
                     GHLPLTIVDLDIVNPYFRSREAAKPMIALGVRVVMPAGEHCSADLPIILPEVKGAIEN
                     QDERVILDVGGDDVGARVMSSMADAFHKDDYEMLFVLNANRPFTSDVKGCMKMKAEIE
                     ASCRLQFTGIVSNTHLIDETVKETVLDGMKLAREFSQESGLPLKFVSAKVKVFENIQP
                     EEIDCAILPLERFMLKPWELESKSR"
     gene            complement(5232..5315)
                     /locus_tag="ENO07_00705"
     tRNA            complement(5232..5315)
                     /locus_tag="ENO07_00705"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(5279..5281),aa:Leu,seq:cag)
     gene            complement(5382..6383)
                     /locus_tag="ENO07_00710"
     CDS             complement(5382..6383)
                     /locus_tag="ENO07_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00513.1"
                     /translation="MKAFITTILIIALILLMLDCSEKVNQPTDLPEPGSINPDTTYVM
                     ITPAWTEADGITFDRPTDVMIGYDRFVYVADRNNDRVVKLTLAGEFVESYQIPNPTQI
                     DQDRALDLLAVNDSSVVLRRSFREGGDFQVVYTAPDVPRPPPIDDTTYAVLFGIAASP
                     FPDKTYSISNFYLNEIYRFDSGDEYVDVQVPSGNAAGRVAAPVAVNSFNIRGQYYIGY
                     TSGRSSFAVQLIEASTGQPVIPFSDSADIYLTTVNGHKDIAVDDLGNIFVTMAAASEV
                     WKFSRDGQLLLRFGQDAPPEDRLDNPRGIDAYQQYIYVADTDNNRVVRFETSTSPQQ"
     gene            complement(6383..>7216)
                     /locus_tag="ENO07_00715"
     CDS             complement(6383..>7216)
                     /locus_tag="ENO07_00715"
                     /inference="COORDINATES: protein motif:HMM:PF00593.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDS00514.1"
                     /translation="VTTIEAGVKYLPSPGRALSISAYFKDISDYIAATQIQPDPRFPD
                     ESYLVYFNLDFAKSRGVELSYTHDFDQYIRGFTNIAVSRAEGERSLPADILRGLEARA
                     EGELFTEVPFDWDVPWQFSIGANIGIPEDKNPELFGMKLPSNWNLYIKYAARAGKRYT
                     PYAEVTDSFGVIQYVPSGETNSELGPYQSWLDLSFRKYFDFGDYRLSLFFEAENLLDH
                     KNVAIINPLTGEEYKQGDTIPGGDNFFEVPPEGYDLPIWNNPTQYLAPRKLRLGMGVS
                     F"
BASE COUNT         1723 a         2014 c         1655 g         1824 t
ORIGIN      
        1 ggtagaggtg ctgcactgcc agggggtatt cctcgcgtac atagatatag gcatcgcgcg
       61 cgcccacagc ataagaggcg actatcattc cctccagcac cgaatgcgga tcgccttcca
      121 tgatactgcg gtccatgaat gccccgggat cgccctcgtc accattgcag atcacatagc
      181 gtacatcaga ttcaacgccg cggcaggtcg cccatttgcg tccggcagga aatccgccgc
      241 cgccgcgtcc gcgtattccc gagagcttga cctcgtcaat tacctcttcg ggcgtcatcc
      301 cggtcagcac tttggccacc gactgataac cgtcccgcat gacataatct tcgatattgt
      361 ccgggtctat atgcccgagg ttgcgaagtg ccagacgcgt ctgttttttg tagaaatcta
      421 tatcatgata tttgagcact ctctcttttg tcttgggatt cttgtacagg agcctttcta
      481 ttatttcacc ctttgccagg gttttctcta cgatatcctt aacctgactg gtcctgaccc
      541 tgttatagaa gataccctcc ggctctatta ccaccagcgg ccctttttcg cacaacccgt
      601 gacacccggt tttcttgaat gactcaaggg tgatgtcgcc gagcttcctc tcggccagaa
      661 tctcctcgaa ttttgcatat atttccctgc atccggcggc cagacatccc gggccgaaac
      721 agaccatcac cttcttcttg agtttggcgt ccttctgcag gcattcttct ttccatgcat
      781 taagcttatc cggtgaatca atcttcatct cgcactccat ccagatatct cttcatcttg
      841 tccacgcgca cattgccata atacttctca tcaataatca cgaccggggc catcgcacat
      901 gctcccacgc agttgacaac ctccagcgtg aagcgcaaat ccggagtggt ctgcccggcc
      961 ttgatcccga gctgatcctc tgcctgctcc agcgccatgg gtgcgccctt gatatggcag
     1021 gctgtaccca tacagatacg gcaggtatgc ttgccgcgcg gctcaaggct gaaggcgctg
     1081 taaaatgtcg ccacgctgta cacctgtgaa agcggaactc ccagtgcgct tgatgtctcc
     1141 tttatcgctt caggcggaag ataatgaaac ttcctctgga tatcctgaag caccgcgatc
     1201 agtgagctgg gtttctgcgg actgtgactg attatttctt tgagctcagc aacgtctatt
     1261 tccggtttca tcctttaact ttctcctctt tggccttccg gccaagctca tatccctcat
     1321 ccagagcttt atagttaatc tgcaaaagat cttttttgcg ccccatcttc tcggtcaatg
     1381 tttccttcag cttgtcgaat tcgacaattc cggttgcgcc cacaaatgct cccaggacga
     1441 ccatgttggt cgcctttccg ctgcccgctt tctgggctat gtcattggcc gggatttcca
     1501 gaacctggat atcatccctg tccgaggtag catcaacaag cgagctgtta ataataagca
     1561 aacctcccgg cttgaccctg ggaccgaact tatccaatga tggacgattg aaaacacata
     1621 ttccctgcgg gcggttgata atcggagagc cgattctttc atccgagacc accacagtgc
     1681 aataagccgt accgccgcgc atctccggac cgtatgacgg tatccaggcc acctgtttgc
     1741 ccgcattcat accggtataa gcgagcagct ggccggcggt cataatcccc tgtcctccga
     1801 atccggcaaa agtcacctca tactgactca cttctgcccc tcctcatcat tatcatagta
     1861 agtgtctttg aacactccca gcgggtagaa agcgatcata ttgtcctcca gccagttctg
     1921 cgcttcatca ggcgtcaaac cccagttggt cggacaggta gaaagtacct caacaaatgt
     1981 aaacctcttc ccttccatct ggtttttgaa tgccttcttg atcgccttct tggccctgat
     2041 gacattatgc ggattatgca ccgaaacacg ctcgatatac gcgggagtcc tcaacgttga
     2101 aaggagctcg ctcatcctga tgggatagcc cgcctggctc acatcacgac ccataggcga
     2161 ggttgtggta acctgcccgg gcatggttgt cggcgccatc tgcccgccgg tcatgccgta
     2221 gatagcgtta ttaacaaaca ctacggtaat cttttcaccg cggttggcgg catgaacgat
     2281 ttcacccatt ccgatcgagg ccaggtcgcc gtcaccctga tatgtaaata caatcagctc
     2341 cggactgacg cgcttaatgc cggtcgcaag cgccggggcg cggccatggg gcgcttccag
     2401 gaaatcggtc ctgaaataat tataaatcaa aaccgagcag ccgaccgggg cgaccccgac
     2461 agtcttgcca cggatgccgt attcatcgat tgtctcagca atcagccggt gaataatacc
     2521 atgagtacat cccgggcaat agtgcgtgac cacatcggtg aatgatttcg gtctttcaaa
     2581 aattttcaat tttgcctctg gcatagtcac ctcagcttta tttctttccg agaattttca
     2641 aaatataatc tttaacttct tcgggcgagg gcacgatacc cccggtgcgt ccatagaatt
     2701 caacgggctt cctgccctgt accgctttgt cgacatcctc aaccatctgg ccagtgctca
     2761 tctcaaccga aagaactgca cgaatatttt ccttccaggc ctgctcacga atctcattct
     2821 ccgggaacgg gaagagagtt atgggacgaa gcaatccgac cgatacgccc tcctcttcca
     2881 tctcatcaat tacagtctgg caaatacgag ccatggtgcc gtaggagatc agcaaaacct
     2941 ggcaatcatc tttcaccttg taaagctcgt aacggacttc ttcctctttc atcttcttat
     3001 acttatccgc cagagcaaag ctgttttttt ccagcgcctg cggatccaag taaagcgact
     3061 tgataattct cggatcgcgg ccctcacatc cggtagcggg ccaatccata gtcatcggat
     3121 catctgtctc cttgatactc tcgggaaact ccaccgcctc catcatctgc ccgatcatac
     3181 catcacccag gaccatcacg ggattaagat atttatgtgc caggtcaaaa gccagcatta
     3241 ccagatcaac cgcttcctga accgttgacg gcgccagcac cagaaggcgg taatcgccat
     3301 gtcccccgcc tttggtcgcc tggaagtagt ctgactgcgc cggaagaatt ccaccgagcc
     3361 cgggaccgcc gcgcacgata tttataaata ccgccggaag atgcgctccg gctatatacg
     3421 atatcgcctc ctgcatcagt gagatgccgg ggctggagga ggtcgtgaat acacgtttgc
     3481 ccgaggcggc cgcaccgaag agcatgttgc ccacggcaac ctccgattcg gcctgaacga
     3541 aaacaccgcc cacctccggc agtcttttta cgagatattc accaacctcg ctctgaggtg
     3601 taatcggata ggcgaagtaa tgcaatcctc cagcacgcac agccgcctca ccgattgcct
     3661 catttccttt catcaaaatt tttgccataa cattcgcacc ttcttcttag tattcgaata
     3721 agacatacct ggtgccatgc gagtgcacct caattgccgc gtcgggacag gttatcgcac
     3781 acagacggca gccgatgcat ctcgacggat cgtgaaccat ggcgtagaaa tatccccgct
     3841 tggtgatctc ctttgacatc gagatgatct gctgcgggca ggccttaaca caaagctcac
     3901 agcctttgca gtaattttta tcaattgtaa ttccgggcat attataaatt ccttctggat
     3961 cgactgccct tatctcgatt tagattccag ttcccacggc ttcagcatga agcgttccag
     4021 cggaagaatc gcgcagtcga tctcctcggg ttgtatattt tcaaaaacct taactttggc
     4081 gctcacaaat tttagaggca gccctgactc ctggctgaat tcgcgcgcca gcttcatacc
     4141 gtccagtaca gtttctttta cggtttcatc aattagatga gtattcgaga ctattccggt
     4201 gaactgaaga cggcaggatg cttcgatctc cgccttcatc ttcatacagc ctttaacgtc
     4261 cgatgtgaaa ggcctgttgg catttaatac gaaaagcatc tcgtagtcat ccttatgaaa
     4321 tgcatccgcc atcgaactca taaccctcgc gccgacatca tcaccgccca cgtcaagaat
     4381 cacccgctca tcctgatttt ctattgcgcc cttgacctcc ggcaggatga tcggcaaatc
     4441 ggcggagcaa tgttctccgg caggcataac cacacgtacc cccaaagcga tcattggctt
     4501 ggccgcctcg cgggagcgga aatatgggtt gacgatgtcg agatcgacaa tagtcagcgg
     4561 aagatgaccc aatctggcaa gataagcggc ataatttacg gcgacttcag tcttgccgct
     4621 gccatatccc ccggcgaata tcaggatgcg tttttcgagg ctggagagct tgtgttcggc
     4681 catctcgagt gctttcgaca aagttaccaa tttcacattc aagatatata cgatacatta
     4741 taaactatta ttagtcaaga aatttaccaa atatttgtga tttccttgag acaaatttca
     4801 catactatct ctgacttttg gagcttcccg aaatgaacct ggaaaaacat ggcaattttt
     4861 atcctgaaag gcttgatttg caataggttt tgctgatttt tcatcaattt ttgcttccga
     4921 atatggcgca gccgaaatcc ctttctgcct tctgaaatcg tcaggaaatc gtgaaagggt
     4981 acaatatcag ccttgagcca cttctcgaat ctatgtcatc tatagattgt tccggtgcag
     5041 ttattaccat cgacttgata ctttttctga tatgatgagg cgcgtgggga tctatatcgg
     5101 gcacgattgc ctttatattt tccgcacgga gcccattccc agcgaattag ccgcaagaaa
     5161 cggggcatgg ttttttgatg atagccggca gttaattcga tgtgatttgt atggttatga
     5221 tcaaacaaaa atgccgaagg cgggatttga acccgcacgc ccttgcgggc actgcgccct
     5281 gaacgcagcg cgtctgccag ttccaccact tcggcatcaa atcgtcaata tgttttacgg
     5341 gcaaccaata tatgcgatcc gaaacttttg gcaagaattt tttactgctg gggcgaagtt
     5401 gaggtctcaa agcggacaac ccggttatta tcggtgtcgg caacataaat atactgctgg
     5461 taagcgtcaa ttccgcgcgg attatccagc cgatcctcag gcggagcatc ctggccgaat
     5521 ctcaaaagca actgaccatc acgggagaac ttccaaacct cggaagcggc agccatggtc
     5581 acaaatatat tgcccagatc atcaactgca atatccttat ggccgttgac agtggtaaga
     5641 tagatatccg ccgaatctga aaacggtatc accggctgac cggtggaggc ctctatcaac
     5701 tgcacagcaa aactggagcg ccccgaggta tagccgatat aatactgccc cctgatattg
     5761 aagctgttga ccgccaccgg tgccgccacc cgtccggcag cattgcccga gggcacctgc
     5821 acgtcaacat actcatcacc agaatcgaag cggtatatct catttaagta gaaatttgaa
     5881 atgctgtatg ttttatccgg gaagggcgag gccgcgattc cgaaaagcac cgcatatgtc
     5941 gtatcgtcaa tcggtggggg acgaggaacg tctggtgcgg tatatacaac ctgaaaatca
     6001 ccaccctcac gaaagctcct tctaagcacc acgctggaat cgttaaccgc cagaagatcg
     6061 agggcacgat cctggtcaat ctgggtcgga ttgggaatct gataggattc cacgaactcg
     6121 ccggccaggg tcagcttgac cacacggtca ttgttgcggt cggccacata cacaaagcgg
     6181 tcataaccga tcatgacatc cgtgggccgg tcgaaggtga tgccatccgc ctcggtccag
     6241 gccggggtaa tcatgacata ggtagtgtca gggttaatgg atccgggctc aggcaggtct
     6301 gtcggctggt tgactttctc gctgcaatcg agcatgagca atataagggc tataatcaat
     6361 attgtcgtaa tgaatgcttt catcagaagc ttacccccat tcccagcctg agctttcggg
     6421 gcgcaaggta ttgtgtcgga ttattccaga tcggaagatc atagccctcc ggaggaacct
     6481 caaagaaatt atcgccgcca ggtatggtgt ctccctgttt atattcttca ccggtaagcg
     6541 gatttatgat ggccacattt ttgtgatcga gaagattttc ggcctcgaag aataatgaca
     6601 gccggtagtc cccaaaatcg aaatatttcc tgaacgagag atcgagccag ctctgatatg
     6661 gtcccagctc ggaattggtt tcgcccgagg gaacatactg aattactcca aatgaatcag
     6721 taacctcggc ataaggtgtg tacctcttcc ctgcccgggc agcatatttt atgtaaagat
     6781 tccagtttga cggcagtttc attccaaata gctcgggatt cttatcttcg ggaatgccga
     6841 tattggcgcc gatcgaaaac tgccagggaa catcccagtc aaacggcacc tctgtaaaaa
     6901 gctcgccctc cgccctggcc tcgagacctc gcagtatatc ggccggaagt gacctttcac
     6961 cttcagcgcg agacactgca atattggtaa aacccctgat atattgatcg aaatcatgag
     7021 tataacttag ctcgactcct ctcgacttgg caaaatccag attgaaataa accagatagg
     7081 actcatcggg aaaacgcgga tcgggctgga tctgcgtggc ggcgatataa tcgcttatgt
     7141 ccttgaaata cgctgatatc gacagggccc gccccggcga gggcaggtat ttcacaccgg
     7201 cttcaatcgt cgttac
//
LOCUS       DSEB01000035            4028 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002142, whole genome shotgun sequence.
ACCESSION   DSEB01000035 DSEB01000000
VERSION     DSEB01000035.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002142"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            92..1180
                     /locus_tag="ENO07_00720"
     CDS             92..1180
                     /locus_tag="ENO07_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006424079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HDS00515.1"
                     /translation="MKNVLDGNAIVYCEGAYNTTNGKTAHGLVRFTKRYKVIAVVDSR
                     YAGRDAGEVLDGVKNNIPLFATIEDAMDHARNNAASIAYFVIGLAPDGGRLSSIARED
                     VKKAIRFGLNVDCGLHDFLSEDPELAQLAMDKRVTLRDLRKPPPRNQLHFFSGKIEEV
                     RCLKVAILGTDSAIGKRTTAWLLVHALQKAGYKAELVGTGQTAWMQGAKFSTIFDTLV
                     NDFVSGEIEHAVWSAWKEVNPDVIVIEGQGSLMNPAYPGGYEILAAGRPNIVILQHAP
                     ARREYDGFPGYVIHPLFKQIGAIEYISDKPVVAITINHEDLKPEDIPGICEKIGREHK
                     RPAFDVLLNGADDLARLVAGYLKQSAGK"
     gene            1200..3578
                     /locus_tag="ENO07_00725"
     CDS             1200..3578
                     /locus_tag="ENO07_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HDS00516.1"
                     /translation="MKDKIEKPIPKQSDPDDPLSVLGVFETLEIGPIKLEKNKVTCPY
                     TLRWGNQSDSTELIYSYEEDVFNPGDDSHLNLASMITAQVALNYGLFCSKLIFHGNFD
                     PVDQRFLRDMAENTAREIYVKKFLEPNPFLTDGFAGLPVIKQKNYLRAGLEFPVTGKS
                     PKSKWQLWASDKHKHCILSSGGKDSLLSFGLLDEIGRENHPVFVNESGRHWFTALNAY
                     RHFKSNIPNTARVWVNSDRVFAWMLRHMPFIRQDFSNVRSDEYPIRLWTVAVFLFGAL
                     PLMRKRGIGRLIIGDEHDTTNRPSHKGIIHYDGLYDQSRYFDDSMSRYYMQKGWAISQ
                     FSLLRQMSEILIETVLVKRYPRLQEHQVSCHAAHKEGERIYPCGKCEKCRRIIGMLAA
                     IDADPKRCGYKQEQIKQSLVDFGKKQVHQEAPGKEHLNYLLQQKGIIRLPDNMQKSVK
                     EHPVIMKLRFTPERSPITGMPVDLRKPLYQILMEYASGAVRRAGRKWEDFDPLTDASL
                     NQPYPFEMLQEKEVKKSSRRKSARLKSYLWSELTWPEVEQRLKEVDIAILPVGAIEQH
                     GPHLTLDVDAFDANYLAHRVAEACSDPKPLILPLVSYGVSYHHEDFPGTISINNDTLS
                     RLVYEIGMSVAGNGIKKLVIINGHGGNSPALNYAAQMINRDAHIFACVDSGETSDVDI
                     YELVETPNDVHAGEFETSTSLATRPHLVRMDEAFKSVPTFSSRYLDFTSKRGVSWYAH
                     TEKISDTGVMGDPTKASAEKGKKMWEIMIAHMVAFVEDLKSMTLEEIYQKRY"
     gene            3585..>4028
                     /locus_tag="ENO07_00730"
     CDS             3585..>4028
                     /locus_tag="ENO07_00730"
                     /inference="COORDINATES: protein motif:HMM:PF02746.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HDS00517.1"
                     /translation="MKINTIHVWPHTFKLKEPYTIAYETIDSTTNVFLRLETSTGVAG
                     YGCAAPDFEVTGETADTVLSACRDIIEPNVKGRDPRRIAYIIEMLQESMRTHPSALAM
                     IDMALHDILGKLAGLPLYVVLGGYRKQIKTSVTIGILPVEETVRRA"
BASE COUNT         1055 a         1013 c         1072 g          888 t
ORIGIN      
        1 caactgctgg attaatcgcc ctgatagaac agcaccgcaa agagggactg ccgaatgatc
       61 gttacgtaat tgtcctgacc gggaagctgt catgaaaaat gtcttagatg gtaatgcgat
      121 cgtttattgc gagggggcct acaacaccac caacggcaaa accgctcacg gactggtacg
      181 cttcaccaaa cgctacaagg tgattgcggt ggtcgattcg cgctatgccg gtcgggatgc
      241 cggcgaagta ctggacggcg tgaaaaataa tattcctctc tttgccacaa tcgaggatgc
      301 catggatcat gcccgcaata atgcggcctc aatcgcatac ttcgtaatcg gtctggcgcc
      361 tgacggcgga cgtttaagct cgattgcccg tgaagatgtc aagaaggcca tccgcttcgg
      421 tttaaatgtc gattgcgggc tgcatgactt tctctccgag gatcctgagc ttgcccagct
      481 tgccatggat aagcgggtca ccttgaggga tttgcgcaaa cctccgccga gaaatcaact
      541 gcactttttc agcggtaaaa tcgaagaggt cagatgcctg aaagttgcaa ttctggggac
      601 cgattcagcc atcggcaaac gcaccaccgc ctggcttctg gttcacgccc tgcaaaaggc
      661 cgggtataag gcggagctgg ttggcaccgg gcagaccgcc tggatgcagg gcgccaaatt
      721 cagcacgatt tttgatacgc ttgtgaatga tttcgtatcg ggtgagatcg aacatgctgt
      781 ctggtcagcg tggaaagagg tgaacccgga tgtcatcgtc atcgagggcc agggcagcct
      841 gatgaatccg gcttacccgg gaggctatga aattctggcg gctggacgcc cgaatattgt
      901 catcctgcaa catgccccag ctcgcaggga atatgacggc tttccgggat atgtcataca
      961 tcctcttttc aagcagatcg gagcaatcga atatataagc gataaaccgg tggtggcgat
     1021 cacgataaat catgaggacc tgaagcccga agatatcccg ggcatttgtg agaaaatcgg
     1081 aagggagcat aagcgtccgg ctttcgatgt ccttttgaat ggcgctgacg atctggccag
     1141 actggttgcc ggatatctga agcaaagcgc gggtaaataa ttatatcggg gaaagacaga
     1201 tgaaagataa aattgaaaag ccaatcccta aacagtccga tccggacgat ccattatcgg
     1261 tcctgggagt gttcgagacc ctggagatcg gtccgattaa gctggaaaag aacaaggtga
     1321 cctgccctta tacgctcagg tgggggaatc aatcggattc aacagaatta atttacagct
     1381 atgaagagga tgtcttcaat cccggcgatg actcgcatct gaatttggcc agcatgatta
     1441 ccgcgcaggt ggccctgaac tacggcctgt tctgcagcaa gctgatcttt catggcaatt
     1501 ttgatccggt ggaccagaga ttcctgcggg atatggcaga aaatacagcg cgcgaaattt
     1561 acgtaaagaa atttctggag ccgaatccat tcttgacaga cgggtttgcc ggcctgcctg
     1621 tcatcaaaca aaaaaactat ctgcgggccg gccttgaatt tcccgtaacc ggcaagagcc
     1681 ccaaatcaaa atggcaatta tgggcgagcg acaaacataa acactgcatt ctctcgagcg
     1741 gcggcaaaga cagcctgtta agctttgggc tcctggatga aatcggccgg gagaatcatc
     1801 ctgtctttgt aaatgaatcc ggccgccact ggttcaccgc tctcaatgcc taccgccatt
     1861 tcaaatcgaa tatacccaat accgcgaggg tctgggtaaa ctctgaccgt gttttcgcct
     1921 ggatgctgcg gcacatgccc tttatccgtc aggatttcag caatgtgcgc tccgacgagt
     1981 acccgatccg cctctggacg gttgcggtct tccttttcgg cgccctgccc ctgatgcgta
     2041 agcgcggtat aggacggctg ataatcggag acgaacatga tacaaccaac aggccctctc
     2101 ataagggcat aatacattat gatggtctct atgatcagag ccgttatttt gatgacagta
     2161 tgtcccgcta ttatatgcag aaagggtggg caatcagcca gttctctttg ctgcgccaga
     2221 tgtccgagat cctgatcgaa acagtgctgg tcaaaagata cccgcgactt caggagcatc
     2281 aggtctcctg ccacgctgcc cacaaagagg gagaaagaat ttatccctgc ggcaaatgtg
     2341 aaaaatgccg acgcattatc ggcatgctcg cggccattga cgcggacccg aaaagatgcg
     2401 gatacaagca ggagcagata aagcagagcc tggttgactt tgggaagaag caggtccatc
     2461 aggaggctcc cggcaaggag catctcaact acctgctcca gcaaaaagga atcatcaggc
     2521 tgcccgataa tatgcagaag tcagtcaaag aacatccggt aataatgaag ctgcggttta
     2581 ctccggaacg gtcgcccata acaggtatgc cggtcgattt gcgcaagccc ctctatcaga
     2641 ttttgatgga atatgccagt ggcgcggtac ggcgggccgg acgtaaatgg gaagatttcg
     2701 atccattaac cgatgcctct ctcaatcagc cttacccctt tgagatgctc caggagaaag
     2761 aagtaaaaaa atcatcacgg cgcaaatcgg cgcgcctgaa atcatatctg tggtcggagt
     2821 tgacctggcc ggaggtagag cagagactga aagaagttga catcgctatt ttgccggtgg
     2881 gcgcgataga acaacacgga ccccatctca ctctcgatgt tgatgcattc gacgccaatt
     2941 atctggcgca ccgggtggcg gaagcctgca gcgatcccaa accgctcata ctgcctttag
     3001 tgtcgtatgg tgtctcatat caccatgaag attttcccgg gacgatcagc atcaacaatg
     3061 acacactttc ccggctggta tatgaaatcg gtatgagtgt cgccggcaac gggataaaaa
     3121 agctggttat aatcaacggg catggcggca acagcccggc attgaattat gcagcccaga
     3181 tgataaacag ggatgcgcat atatttgcct gtgtcgattc cggtgaaacc agcgatgtag
     3241 atatttatga gctggtggaa actcccaatg atgtccacgc cggcgaattt gaaaccagca
     3301 cgagcctggc gacccggccg cacctggtaa ggatggacga ggcctttaaa agcgtgccca
     3361 ctttctccag ccgctatctt gatttcactt ccaaacgcgg tgtttcatgg tatgcacaca
     3421 ctgaaaagat ttccgataca ggcgtcatgg gagaccccac caaggccagc gcggaaaaag
     3481 gcaagaagat gtgggagatt atgatcgccc atatggtcgc ctttgtcgag gacctgaaaa
     3541 gcatgacgct ggaagaaatc tatcagaaaa gatattgaaa atatatgaag atcaacacga
     3601 tacatgtctg gccgcacaca ttcaaactca aggaaccata tacgattgcc tatgagacca
     3661 tcgattcgac caccaatgtt ttcctgcggc tggaaaccag caccggtgtt gccggttatg
     3721 gttgcgcggc gccggatttc gaggtaaccg gcgaaaccgc agatactgtg ctgtcggcct
     3781 gccgcgatat tatcgagccg aatgttaaag gcagggatcc gcgcaggatc gcctacatca
     3841 tcgagatgct gcaggaatcc atgcgcacgc acccctccgc cctggccatg atcgatatgg
     3901 cgcttcatga catcctggga aagcttgccg gtcttcccct gtacgtggtt ctcgggggat
     3961 acagaaaaca gatcaagacc agtgttacaa tcgggattct gcctgttgaa gagactgtcc
     4021 ggagagca
//
LOCUS       DSEB01000036            3022 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004057, whole genome shotgun sequence.
ACCESSION   DSEB01000036 DSEB01000000
VERSION     DSEB01000036.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004057"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(94..489)
                     /locus_tag="ENO07_00735"
     CDS             complement(94..489)
                     /locus_tag="ENO07_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00518.1"
                     /translation="MNAPSHDIKDILGADSSLGLVFRTNLFVSEMPASPDECVAIYDT
                     GGSDPEENFVYKRPTIQVRIRGAKGKYRDAYALAKDIQDSLHVVANYTINSTRYIAIW
                     AQGDILFLGYDDNHRPLLTVNFRMHRTNA"
     gene            complement(756..1121)
                     /locus_tag="ENO07_00740"
     CDS             complement(756..1121)
                     /locus_tag="ENO07_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDS00519.1"
                     /translation="MAVKSRVTFTGLNRVLANLNREISGIRGRTRAGMQKAALIVQRR
                     SMELTPVKTGNLRASAYHTMIDLPKGPGAEIGYTASYAPFVHEIDRFHNIGQWKFLET
                     ALKDKQGEVLEAIRSKVKV"
     gene            complement(1112..1471)
                     /locus_tag="ENO07_00745"
     CDS             complement(1112..1471)
                     /locus_tag="ENO07_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00520.1"
                     /translation="MSRSKLITSLLKQTAVYWANPQPDGFGGRSYDVAAEIDCRWEER
                     QDIFVDSQGREVRSQAVVYVGQDLDMGGYLFLGELSDLTAEERADPLVCDSAFEIRSK
                     ESVPNLKTDIYVRKVWL"
     gene            complement(1468..1830)
                     /locus_tag="ENO07_00750"
     CDS             complement(1468..1830)
                     /locus_tag="ENO07_00750"
                     /inference="COORDINATES: protein motif:HMM:PF13262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HDS00521.1"
                     /translation="MAFRIQENEVLEIMDTALTQAQITPFLQAANLVVTDKLTGEYRT
                     DLLAEIERWLAAHLAAMLDPQTSREKLGDADATYEGKTGMGLDFTRYGQQVKVLEYKG
                     ILAELGQSKGPAEAKTIT"
     gene            complement(1845..2510)
                     /locus_tag="ENO07_00755"
     CDS             complement(1845..2510)
                     /locus_tag="ENO07_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00522.1"
                     /translation="MRKINGKMHELILITGAARSGISLIGDVLQLCGAHNAVSDRDEI
                     RETMVKPILRGLRADCSGQNPLPSISQCLKVSKIVALPWRRNIERTVAGKEGQIYYAS
                     PLSCLIWPIWTQAFPRARWIIVRREDQDIVSACIRTGYMNGYKNKYGWQEWVDWHKRR
                     FPEIVSANASTWTIWPHKLMTGDLSELKDLILALGLRWDIADVEDFLAPVLWKGGVFE
                     VKK"
     gene            complement(2482..>3022)
                     /locus_tag="ENO07_00760"
     CDS             complement(2482..>3022)
                     /locus_tag="ENO07_00760"
                     /inference="COORDINATES: protein motif:HMM:PF02709.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00523.1"
                     /translation="IKAGAQGEYNPSRLFNAGAARARGKYLVITNPECFHKSDILGGM
                     DKLLSRADYVVCACLSLADTAVRLKTFDDLKPQVHMWYQHTKHRDVRYHFCTALSREN
                     YRKIGGFDERYAEGIGYDDNSFLDRVTKAGLKILVSDELLVYHQKHEKLTEVIPRNVY
                     MQKLRRNRGLFEKDKRKNA"
BASE COUNT          648 a          887 c          681 g          806 t
ORIGIN      
        1 cagctatggc cgtgaaggtc tcggagctcg ctccgtctcc acgtttgaac tgagttccca
       61 cacctgctac agcgttactc atagtaacct ccttcatgca tttgttctat gcatcctgaa
      121 attgaccgtc aaaagtggtc tgtgattgtc atcatatccg aggaacagga tgtctccctg
      181 tgcccagatt gctatgtatc gagtcgaatt gattgtgtaa ttcgccacga catgcagact
      241 gtcctggatg tccttggcca gcgcatatgc atcccggtat tttcctttgg cgccgcggat
      301 tctgacctga atggtgggcc gcttgtaaac gaaattctct tccggatccg agcctccggt
      361 gtcatagatg gccacacatt catccggact ggccggcatc tccgaaacaa acaggttcgt
      421 cctgaatacc agtcccagac tggaatcagc acccagaatg tcttttatgt catggcttgg
      481 tgcgttcata tgctcacctc aaaacaaaaa ggccggatcc gggagtatgc ccggcatccg
      541 gccttctgtt ctccagaact tccgaccttt ggacttcaaa caactataat tcttatgcca
      601 atttgatcgg taccgatatc tcctttttgc atttatcgca tttggtcagc gctataccgt
      661 ttattattct gaccttgcgc actctaatta tggtcacact attggaggcg gtctcgatta
      721 tctttctgcc gcaatgctcg caacgataaa gcatatcaga ccttgacctt gcttcttatg
      781 gcttccagca cctcgccctg tttatccttc aatgcggttt ccaggaactt ccattggcca
      841 atattgtgga agcggtctat ctcatgaaca aaaggcgcat aggaagctgt atatccgatc
      901 tcggcgcccg ggcctttcgg cagatcgatc atcgtatggt atgccgaggc cctgaggttg
      961 ccggtcttga ccggagtcag ctccatggat ctccgctgaa caatcagggc tgccttttgc
     1021 atccctgccc gggtgcgtcc cctgatgccc gatatctccc ggtttagatt tgccaggacc
     1081 ctgttaaggc ccgtgaatgt tacccggctt tttacagcca taccttcctc acatatatgt
     1141 ctgtcttcaa attagggacc gactccttcg agcgaatctc aaaagctgaa tcacacacca
     1201 gaggatcggc ccgttcttct gccgtcaaat cggatagctc gcccaggaag aggtaaccgc
     1261 ccatatccaa atcctggccc acatagacaa ccgcctgcga tctgacctcc ctcccctgag
     1321 aatcgacaaa tatatcctgg cgctcctccc atctgcaatc gatctcggct gctacatcat
     1381 aggatcttcc gccgaatcca tcaggttgcg gattggccca ataaacggcc gtctgcttaa
     1441 gcagactggt tatcaatttg gatctgctca tgttatggtc ttcgcctccg ccgggccctt
     1501 gctttgacca agctctgcca ggatgccctt atactcgagc accttaacct gctggccata
     1561 gcgcgtaaaa tccagtccca taccggtctt gccctcatag gttgcatccg catctccgag
     1621 cttctccctt gaggtctgag gatccagcat ggccgcaagg tgagcggcaa gccagcgttc
     1681 gatctctgcc agaaggtctg ttctatattc gccggtcagt ttatcggtca ccaccaggtt
     1741 ggcagcctgc agaaatggag tgatctgtgc ctgggtcaaa gccgtatcca tgatctccag
     1801 gacctcattt tcttgaattc taaatgccat aatggcctcc gctgttattt cttgacctca
     1861 aatacgcccc ccttccacag taccggcgcc agaaagtcct ccacgtcggc aatatcccat
     1921 ctcagtccca gcgccagaat aagatctttt agttccgaca gatcaccggt catcagctta
     1981 tgcggccaga tcgtccatgt gctcgcgttc gcacttacga tctccgggaa acgtcttttg
     2041 tgccagtcga cccattcctg ccagccatat ttattcttat acccgttcat atatccggtc
     2101 cttatgcaag ccgagacgat atcctgatcc tcacgtcgta ctatgatcca gcgcgcgcga
     2161 ggaaatgcct gagtccagat cggccagata aggcagctga gcgggctggc ataatatatc
     2221 tggccctctt tacccgctac ggttcgctct atatttcttc tccagggcag ggcgacaatt
     2281 ttagatactt tcagacattg gctgattgaa ggcaatggat tctgcccgct acaatccgcc
     2341 cgcaggcccc tcaatatggg tttgaccatg gtttccctga tttcatcgcg gtcggagacg
     2401 gcattatgcg ctccgcagag ctgcaggaca tccccgatca gggagattcc ggagcgtgcc
     2461 gccccggtta tgagaatcag ttcatgcatt tttccgttta tctttctcaa acaggccccg
     2521 gtttcgcctc aatttctgca tatacacatt ccgcggaatg acttctgtca atttctcatg
     2581 cttctggtga tacaccaaca gctcatccga taccaggatc ttgagaccgg ctttggttac
     2641 cctatccagg aaggaattgt cgtcgtaccc gattccttcc gcgtagcgct catcaaagcc
     2701 gccgatcttc ctgtagttct cacggctcaa cgctgtacag aagtgatagc gcacatcgcg
     2761 atgtttggtg tgctgatacc acatgtgcac ctgcggtttc agatcgtcga aggtcttcag
     2821 ccgaacagct gtatcggcca ggctcaaaca ggcgcatacc acataatctg cccgactcaa
     2881 caatttgtcc attccgccca gtatatccga cttgtggaag cattccgggt tggtgatcac
     2941 cagatattta ccccgggccc tggcggctcc ggcattaaag agccgtgagg gattatattc
     3001 cccctgagct cctgccttga ta
//
LOCUS       DSEB01000037            5520 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001055, whole genome shotgun sequence.
ACCESSION   DSEB01000037 DSEB01000000
VERSION     DSEB01000037.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5520)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5520)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5520
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001055"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..941
                     /locus_tag="ENO07_00765"
     CDS             <1..941
                     /locus_tag="ENO07_00765"
                     /inference="COORDINATES: protein
                     motif:HMM:PF02687.19,HMM:PF12704.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDS00524.1"
                     /translation="YISPEYTEWNKTVIYKDKRLVAPIVGVWPEFGEMRNIFARMGGR
                     FINQNDLDDRRRVCFLGNELKEQIFGEEMDPVGQIIEISGVPFKVIGVLKEKQQDSSY
                     NSRDSRKIIIPSTTFVALYGQNYVDNVVWKAESQDVHQHVKQKYFEIMGKRHQFDPSD
                     RQALMTWDTTENTEFFTYFFWGFRVFLYLISIATLIVGGIGVANIMYVVVEERTREIG
                     IKMALGAKSGFVLRQFLFETMFLTAFGGLMGFLFSATIVKLFPRLNLEAYVGIPEISS
                     FVSLGTIAALLIIAVLAGYFPARRAATMDPVKALKI"
     gene            944..2206
                     /locus_tag="ENO07_00770"
     CDS             944..2206
                     /locus_tag="ENO07_00770"
                     /inference="COORDINATES: protein
                     motif:HMM:PF02687.19,HMM:PF12704.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDS00525.1"
                     /translation="MLNIKLVFKMFWREFKAQKKRMFLTILGIVWGTMSITMLLAFGD
                     GLRNQLDINQKGMGEGIMIVWGGQTSMPYKGLPRGRPIRFEKDVVEFLKERIPQIEMI
                     GGEYTRWGQSVANKSKMFNRMVNGVYPDYEIMRNMIPEMGGRFINQLDMDLKRRVVFL
                     GNELADDLFGANIPREEIVGQTIEINGMKFTVIGVLKKKMQMGMYSGPDWNRAVIPAT
                     TFHGIYGHRYFNNLVVKPRDLSKTEFVEKRIIEELSGKYKFDPADESTIGVWNFIENV
                     KTFNNVMLGMQIFFGLIGGLTLLVAGVGVANIMYVAVKERTTEIGVKMALGAKRFQVM
                     AQFMLEALLIAFIGGGLGILFSVVITRILGSIDINNEALLWLGKPNISTTIAIITASI
                     LGIIGLVSGFFPSRKAASVNPVESLRYE"
     gene            2218..2919
                     /locus_tag="ENO07_00775"
     CDS             2218..2919
                     /locus_tag="ENO07_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_798374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDS00526.1"
                     /translation="MNNTMIYMKDIIKFYNTGTVKVKALKGIDLDIRGGEFVSIVGPS
                     GSGKSTLMNIAGCLDVPTGGEYILDGERVENLEPNELAEIRNKKVGFVFQNFNLLPYA
                     TAFENVELPLVFANVPAKKRRERVAELLDRVGLGDRMDHRPNELSGGEMQRVAIARAL
                     ANRPRLILADEPTGNLDSKSGADIINLFEELWKAGSTVLVITHDQSIAKRTNRVVKLK
                     DGLIVSSLENGNGDH"
     gene            3033..4067
                     /locus_tag="ENO07_00780"
     CDS             3033..4067
                     /locus_tag="ENO07_00780"
                     /inference="COORDINATES: protein motif:HMM:PF13205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00527.1"
                     /translation="MNLKSIILVLFISGLGISCSKSPTGPDDPLDRFIAGVSLGSDTL
                     ITNLTEPIVISFHLNMDRQSVEEAFSSEPAFDYDVHWTTFPICNPNDSTCYPKFYMFY
                     IYPQEPFLPNTTYSCMIDSSAHDIIGDHLPRPYEFQFTTESSRLLRITPEYDGVDTLD
                     AFPRAIKLWFNNSIDLSTIQEPFTASPSFDYELYGISDKKNVFEYRITSPLRSQSEYQ
                     IVIDAEIFDIHENRVLTEIDGGFSTGKLAIVYFYPNPGVNLKDTYPIIQVRFNTVMDR
                     RTVQEAFSFNDGVIEIPGEFVWFTDKAMQYFPDSGLPEGETYTFSVSTGAADTYGATL
                     DEAFSYSFTS"
     gene            4191..4829
                     /locus_tag="ENO07_00785"
     CDS             4191..4829
                     /locus_tag="ENO07_00785"
                     /inference="COORDINATES: protein motif:HMM:PF13505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00528.1"
                     /translation="MKRLLFILFLLFVLSASGSAGIFGSLGGGLGATMPQGEFNEYTK
                     TGFSIMGYGSIGVLKMPYMQLRLGLQGVFFERDNRNVVFEGDPEDIFTETYTNDLLKG
                     TLGLEFSKRLAAIQPYGGGGVGIYYFESKTELKDPAGEVIASNKLDSKTKFGWNLNGG
                     IRIFMFPRVAFDFNIQYDIVQDLEQYSEEEVVSFNSEFLSLFAGVSIPLGLF"
     gene            complement(4826..5314)
                     /locus_tag="ENO07_00790"
     CDS             complement(4826..5314)
                     /locus_tag="ENO07_00790"
                     /inference="COORDINATES: protein motif:HMM:PF01927.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00529.1"
                     /translation="MSNSSLQKKFIADEMVGKLARLMRMLGFDTLYFRQIEDSDLIDL
                     SLKEKRTILTRDTKLVEKVMVKDFVLIESDDPDKQLSELMKKSGLTPDENDFLSRCLD
                     CNTVLEDIPKGKIENRVWPYTYETHELFKICPRCDKIYWEGNHVKAMREKLRKIGVYR
                     SD"
BASE COUNT         1409 a         1182 c         1394 g         1535 t
ORIGIN      
        1 aatatatttc gcccgaatat acggaatgga ataaaacggt tatatacaag gataaaagac
       61 tggttgcgcc gattgtcgga gtctggccgg aattcggcga gatgcggaat atttttgccc
      121 gcatgggggg acgttttatc aatcagaacg acctggatga ccgtagacgt gtctgtttcc
      181 tgggcaacga gttgaaggag cagatattcg gcgaggagat ggacccggtc gggcaaatta
      241 tagaaatttc cggtgtacca ttcaaggtaa ttggtgtact caaggaaaag cagcaggatt
      301 catcctacaa cagtcgtgac agcaggaaga taattatccc ctcaacaacc tttgtggccc
      361 tttatggaca gaattatgtc gataatgttg tctggaaagc ggagtcccag gatgtccatc
      421 agcatgtcaa acaaaaatat ttcgaaatta tgggcaagcg gcatcagttc gatccctccg
      481 accggcaggc tctgatgacc tgggatacca ctgaaaacac ggaattcttt acatattttt
      541 tctggggctt cagggtattt ctgtatttga tctcgattgc aacccttatt gtgggaggaa
      601 tcggggtcgc taatatcatg tatgttgttg tggaggaaag gacacgggag attggaatta
      661 agatggctct gggggcaaaa agcggttttg tgctgcggca atttttgttt gagaccatgt
      721 tcctgacagc ttttggcggc ctgatgggct tccttttttc tgcaactatc gttaaactgt
      781 tcccgcgtct aaatttggaa gcatatgtag gtattccgga aatatcgtcc tttgtgagcc
      841 tcggcacaat agccgcattg cttataatcg cggtcctggc gggatatttt cctgcccgca
      901 gggcggcgac catggatccg gtaaaagctt tgaagattta gatatgctga atattaaact
      961 tgtatttaag atgttctggc gcgaattcaa ggcgcagaag aaaaggatgt tcctgactat
     1021 cctcggtatt gtctggggaa ctatgtccat aactatgctc ctggccttcg gggacggttt
     1081 gcggaaccag ttagatatca accagaaagg catgggcgag ggaattatga tcgtctgggg
     1141 cggccaaacc agcatgccgt ataagggtct tccgcggggg cgtccgattc ggttcgaaaa
     1201 agatgtggta gaatttctga aagaacgtat cccccaaatt gaaatgatcg ggggtgaata
     1261 tacccgctgg ggacaatcgg tggctaacaa gagcaagatg ttcaaccgca tggtcaatgg
     1321 cgtttatccc gattacgaaa tcatgcgcaa catgattccg gaaatggggg gacgtttcat
     1381 aaaccagctt gatatggatt tgaaacggag agttgttttc ctcggaaatg aactggccga
     1441 tgatttgttt ggcgccaata taccccggga ggaaattgtg gggcagacaa ttgaaattaa
     1501 cggcatgaag ttcacggtga tcggcgttct gaagaagaag atgcagatgg ggatgtattc
     1561 cgggccggat tggaatcggg cggtgattcc ggccaccaca tttcacggca tctacggcca
     1621 tcgttatttt aataatctgg ttgtcaagcc gcgcgatcta agtaagacgg aatttgtcga
     1681 aaaacgcatc atcgaggagt tatcgggcaa gtacaagttc gatcctgctg atgaatccac
     1741 tatcggtgtc tggaatttta tcgagaatgt caagacgttc aataatgtca tgctggggat
     1801 gcagatattt ttcgggttga tcggcgggct cactcttctg gtcgcgggtg tcggcgtggc
     1861 aaatataatg tatgtcgcgg taaaggagcg cactactgaa atcggggtca agatggcttt
     1921 gggcgcaaag agattccagg tcatggcgca gttcatgctg gaagccctgc tgatagcatt
     1981 tattggagga gggttgggca ttttattctc ggtagttata accaggatac tggggtcaat
     2041 agatatcaat aatgaagccc tgctatggct ggggaaaccg aacatttcca ccacgattgc
     2101 gattataaca gcaagcattc tcggaataat cggcctggtt tccgggttct tcccatcccg
     2161 gaaggcggct tcggtcaatc ctgtggaatc attgcgatat gaatagaggt tcagattatg
     2221 aataacacta tgatctatat gaaagatata atcaagttct ataatactgg tacagtcaag
     2281 gtcaaggcct tgaaaggcat cgatttggat attaggggag gcgagttcgt ttccatcgtg
     2341 gggccctcgg gttccggaaa atccactctg atgaacattg ccggatgcct ggatgtcccc
     2401 accggcggtg agtatattct ggatggcgag agagttgaga acctggaacc aaatgagctg
     2461 gccgagatca ggaacaagaa ggtgggattc gtatttcaga atttcaatct tctgccatat
     2521 gccacggcct tcgagaatgt cgaactgccg cttgtattcg ccaatgttcc tgctaaaaaa
     2581 cggcgtgaga gagtggccga gctgctcgac cgtgtcggcc tgggcgaccg catggaccat
     2641 cgccccaacg agctttccgg gggcgagatg cagcgagtgg ccatcgctcg ggctctggcc
     2701 aacagacccc ggctgatcct ggccgatgag ccgacgggaa atctcgattc caaatccggg
     2761 gcagatatta taaatctgtt tgaagagcta tggaaggcgg gttcgaccgt tctagttatt
     2821 acccacgatc aaagcattgc taaaaggacg aatcgcgtgg tgaaactcaa ggacggcctg
     2881 atagtgtcct cgctggaaaa cggtaacggc gatcattaat ttcagtatta tccgcaccgg
     2941 gcggaatatc tcaactgtat tttgactcaa gcaaatttag aatctctctt gtataataaa
     3001 cgggagcgat tatatttcct cagggtgcaa ttatgaatct gaaatcgata atattagtcc
     3061 tgtttatttc aggtctgggg atttcctgtt cgaaatcgcc aacaggtccg gatgatcctt
     3121 tggatcgttt tatagcaggc gtttccctcg gttcggatac tcttattact aatttgaccg
     3181 aaccgattgt aattagcttt catctgaata tggacaggca gtcggtggag gaggcatttt
     3241 catccgagcc tgctttcgat tacgacgtac actggaccac ctttccgatc tgcaacccta
     3301 atgattccac ctgctatccg aaattctaca tgttctacat ttatccgcag gaaccgtttt
     3361 tgcctaatac aacatatagc tgtatgattg acagcagcgc acacgatatt atcggcgacc
     3421 atctgccacg accatatgaa ttccaattta cgacggaatc atcaaggctc ctgcgcatca
     3481 cacctgaata tgacggggtg gatacactgg atgcatttcc gcgggcaatt aaactgtggt
     3541 ttaacaattc gattgacctt tcgactattc aagagccgtt tacagccagc ccctcatttg
     3601 attatgaact ttatggaatc tccgacaaga agaatgtctt tgaatatcgg atcaccagtc
     3661 cattgaggag ccagagcgaa tatcagattg tgattgatgc agagattttc gatattcatg
     3721 agaatcgggt actaacagag attgacggcg gcttcagtac tgggaagctt gctattgtct
     3781 atttttatcc caatccgggt gtcaatctca aggatacgta cccgattatt caggtcaggt
     3841 tcaatactgt catggacagg cggaccgttc aggaggcttt cagttttaat gacggagtga
     3901 ttgaaatccc cggggaattt gtctggttta cggataaggc catgcaatat tttcccgatt
     3961 ctggcctccc cgagggagaa acttatacct tttcagtatc tacaggcgcc gccgatactt
     4021 atggagctac tctggacgag gcgttttcgt attcttttac gtcatagtgc cgatattttt
     4081 gcaaataata tgcaatcttg tgattattgt tgacaggaca cctgtgattt aataaaattt
     4141 ttggaatttg gagatcgtca gtttacttta ttgcctgtgt ggatgagaat atgaaaagat
     4201 tgctgtttat attatttctg ctttttgttc tgtcggcttc gggatcggcc gggatattcg
     4261 gctcacttgg cggcgggctg ggggccacca tgcctcaggg tgaattcaat gaatatacca
     4321 agaccggttt ttctattatg ggttacggca gcataggcgt gctgaaaatg ccctatatgc
     4381 agcttcgtct tggtttgcag ggggtcttct tcgagcgtga caaccgcaat gttgtctttg
     4441 agggcgatcc ggaggacatt tttaccgaga catataccaa tgatctcctt aagggcaccc
     4501 ttggtctgga attctccaaa aggctggcgg ccattcaacc gtatgggggc ggcggggtag
     4561 gtatctacta tttcgagagc aaaaccgagc tcaaggatcc cgccggcgaa gtcatcgcct
     4621 ccaataagct cgacagcaaa accaaattcg gctggaatct taacggcggc atcagaatat
     4681 ttatgttccc cagagtagct ttcgatttca atatccagta tgatatcgtg caggacctcg
     4741 agcagtattc ggaggaggaa gtggtctcgt ttaattcgga atttctctca ctttttgcag
     4801 gggtctccat tcctctgggg cttttctaat ccgatcgata gactccaatt tttctaagct
     4861 tctcacgcat cgccttaaca tgattcccct cccagtatat cttatcgcat cggggacaga
     4921 ttttgaagag ctcgtgcgtt tcatacgtgt acggccagac ccggttctct atcttgccct
     4981 tagggatatc ctccagaaca gtattacaat ccagacagcg gctcaggaaa tcattctcat
     5041 ccggggtcag tcccgatttc ttcatcaatt ccgacagttg tttatccgga tcatcactct
     5101 cgatcaaaac gaaatccttg accatcactt tttccaccag ctttgtatct cgggtcagaa
     5161 ttgtacgctt ctctttcagg gagagatcga tcaaatcgct gtcttcaatc tgcctgaaat
     5221 aaagggtgtc aaaaccgagc atcctcatca ggcgggccaa tttgccgacc atctcgtcag
     5281 caatgaactt cttttgtaaa ctactgtttg acatagtata agaccagcag tataattaca
     5341 ggatatattt cttgccggca tttgtaaagt cataatttga agggaactga agaccgtttc
     5401 gtatgtttgc tctttaaatg tatatgaaat cgaagttttt gaaaagatta gagcggctga
     5461 tcgcttattt caagcgccat aaaaagtatc tttattgggg cgttctggtt acagtactga
//
LOCUS       DSEB01000038            2614 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1005585, whole genome shotgun sequence.
ACCESSION   DSEB01000038 DSEB01000000
VERSION     DSEB01000038.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1005585"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..793)
                     /locus_tag="ENO07_00795"
     CDS             complement(<1..793)
                     /locus_tag="ENO07_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00530.1"
                     /translation="MRELFRIALLLGLAAILSCSSHVRDYGTAEADLPPVEETYDEQE
                     EYYFTSDEFEDSTDILEFEDPQAPESQITDEDIRYKFSLAEEYYAFGVAANLKKNWDE
                     AQYNFEKAIEILTSMDLTFDTTDAEMIILEHSYRRLMNEIASDYKLTLFNLNILSEEA
                     SPFAIDEKFALLDSLTGFARDTIVTTDAGIQEVTYDMPIVLNDRVKKWIIYFQTSARK
                     NMEIWLARSGMYIPLMEKILEEEGVPHDLVYLPIIESGFSSRAYSS"
     gene            complement(942..1871)
                     /gene="miaA"
                     /locus_tag="ENO07_00800"
     CDS             complement(942..1871)
                     /gene="miaA"
                     /locus_tag="ENO07_00800"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:PRK00091.2-2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDS00531.1"
                     /translation="MEGRDKKKVLGLLGPTGVGKTGLAIELCQRLNGEVVSCDSRQIY
                     KELDIGTAKPDEEEQRQVRHHLINIAEIGEVFSAYRYRQMALNAIEDIFERGKQPLVV
                     GGTGLYYRALTEGFFEVPENDQEFRQKLEEEAEEYGNERLWNRLNTVDPESAEKISVS
                     DRFRIIRALEIYELSGIKKSELSEKGEYPESCYKFITFGLNVPRRELYERINSRVDKM
                     LEEGWIEETERLISASKLNSRNFQKFMGYNYLYKYVKGQIDRKEGIAKIKQAHRNYAK
                     RQLTWFKRVSGITWISAKDQEKAEKIVELFTKQ"
     gene            complement(1861..2580)
                     /locus_tag="ENO07_00805"
     CDS             complement(1861..2580)
                     /locus_tag="ENO07_00805"
                     /inference="COORDINATES: protein motif:HMM:PF08676.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00532.1"
                     /translation="MHTGKSPASLENIRPTPERKTGDASESLASKPADERSTIVEKST
                     REESVFELTPYRVEQFAHMFIIAFSRDQITIIDQHAAHERILYERALKSFEHQKMSSQ
                     KLLMPVNIEMEPGMISGAEEYFELLNNLGFELERFGPRSLAIYAVPAVASGKNPETLV
                     RDLLSDLMEYGDESEDRFKLAAQRFACHAAVRGGDNLSEEMMRGLVRELFKSENPYIC
                     PHGRPTIIRYTVEGLRSQFGR"
BASE COUNT          629 a          688 c          530 g          767 t
ORIGIN      
        1 cacttgaata ggcgcgcgat gaaaatccgc tttcgataat tggaaggtag accagatcat
       61 gaggcacacc ttcctcttct aatatcttct ccatcaaggg aatatacatg cccgaacgtg
      121 ccagccagat ttccatattt tttcgggcag aggtctgaaa atatattatc cacttcttca
      181 cccggtcgtt caagacaatc ggcatatcgt aagtgacttc ctgaataccg gcgtcagtcg
      241 tcacgatcgt atccctggca aaaccggtca ggctgtccag gagtgcaaac ttctcgtcga
      301 tcgcaaaagg cgaggcctcc tccgacagga tattcaggtt aaacagtgtc agtttgtaat
      361 ccgaggcaat ttcattcatc aacctgcgat agctatgctc gagtatgatc atctcggcat
      421 cggtggtatc gaacgtcaga tccatggagg tcaaaatctc aattgccttc tcaaaattat
      481 actgggcttc atcccagttc tttttcaggt tggcggccac gccaaaagca taatactcct
      541 cggccagact gaatttatat cttatatctt catccgtaat ctggctttcc ggagcttggg
      601 gatcctcaaa ttctaaaatg tccgtcgagt cttcaaactc atccgatgtg aaatagtatt
      661 cctcctgctc atcatatgtc tcctctacgg gcggcaggtc agcctctgct gtgccatagt
      721 cacgcacatg ggaactgcat gatagtatgg ctgcgagtcc taatagtaag gcgattctaa
      781 acagctctct catcaaaata cccttccgat aagttctatc cttagcaacc tattgattga
      841 caacataatg aaaaaattgt taagtctctt tgtcaagata tttttaaagt tatccgtatc
      901 agctatgcta tttttataaa tcggcaaatt tgccggataa cttactgctt agtaaatagt
      961 tccactatct tttcagcttt ttcttgatct ttagccgaaa tccatgtaat cccgcttacc
     1021 cgtttgaacc aggtcagctg gcgtttggca tagtttcggt gagcttgttt tatctttgcg
     1081 ataccctcct tacggtctat ttgtccttta acatatttat ataaataatt ataccccata
     1141 aacttctgaa agtttcttga atttaatttg gaagccgaaa ttaatctctc ggtctcctcg
     1201 atccaacctt cttccagcat cttatccaca cggctattta tgcgttcata cagttccctt
     1261 cgaggcacgt tcagcccaaa tgtgataaat ttatagcagg attcgggata ttctcctttt
     1321 tcggacaatt ccgatttttt tatgcccgac agctcataaa tctccagcgc ccgaattatt
     1381 ctgaatctgt cggaaactga tattttttct gctgactccg gatcgaccgt attgagccga
     1441 ttccagagac gctcatttcc atattcctcg gcctcttctt ccagcttttg cctgaactcc
     1501 tgatcatttt cgggcacctc aaaaaaaccc tcagtcaggg cgcgataata aagtccagtg
     1561 cccccgacca caagaggctg ctttcctctc tcgaagatat cctcgattgc gttcaatgcc
     1621 atctgcctat atctatacgc tgaaaagact tcccctattt ctgctatatt aattaaatgg
     1681 tgtcggacct gcctttgttc ctcttcgtca ggcttggcgg tgccgatatc gagttcctta
     1741 tagatttgac gcgagtcaca ggatacgacc tctccattta acctttgaca caattcaatg
     1801 gccaggccgg ttttgccgac tccggtcggc cccagaagcc ccagcacttt tttcttgtct
     1861 ctaccttcca aactgcgatc tcaatccctc gactgtatag cggattatgg ttggacggcc
     1921 atggggacat atatatggat tttccgactt gaacagctcc cgcacaagcc cccgcatcat
     1981 ctcctcggaa agattatctc ctccacgcac cgcggcatgg caggcaaaac gctgcgccgc
     2041 aagcttgaag cggtcctcgg actcatcgcc atattccatc aaatcagaaa gcagatcccg
     2101 caccagtgtc tcgggattct tgccggaggc cacggcggga acagcatata tcgccagcga
     2161 ccgcggcccg aaccgttcta attcaaagcc gagattattc aaaagctcga aatactcctc
     2221 cgccccggag atcatgccgg gctccatttc gatattcacc ggcattagaa gcttctgcga
     2281 ggacatcttc tggtgttcga atgatttaag cgcgcgctca tatagaatcc gctcatgggc
     2341 ggcatgctga tctatgatgg taatctggtc gcgcgagaaa gcaataataa acatgtgggc
     2401 aaattgctcg actcgataag gtgtgagctc aaagacagat tcttcccttg tacttttttc
     2461 gactatcgta ctccgctcat cggctggttt cgatgcaagg ctctcgcttg catctcctgt
     2521 ctttctctcg ggtgtcggcc tgatattctc aaggctggcc ggggattttc cggtgtgcat
     2581 cttgaatgcc cgctggtttt cacttatata tgag
//
LOCUS       DSEB01000039            3922 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002268, whole genome shotgun sequence.
ACCESSION   DSEB01000039 DSEB01000000
VERSION     DSEB01000039.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002268"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            182..3310
                     /gene="secA"
                     /locus_tag="ENO07_00810"
     CDS             182..3310
                     /gene="secA"
                     /locus_tag="ENO07_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDS00533.1"
                     /translation="MLDTLIKKVFGTKHERDLKKINPIVDEINEIYPTLEDVPQEELI
                     KRTEEFKAEAKKVWDELEQQAEEFEWDTKERKEKFKEGIDEYLDTILPEAFAMVKEAC
                     RRLKGQTWEAGGMNFTWDMIPYDVQLFGGIVLHQGKIAEMATGEGKTLVATMPVYLNA
                     LVGRGVHIITVNDYLARRDAEWMGEVYKYLGLTVGVIQHGQDVDERRAQYNCDITYGT
                     NSEFGFDYLHDNMGWRVEDNRQRGHFYAIVDEVDSVLIDEARTPLIISGPVESTIDQA
                     FRDMQPLVERIAKKQTLMASQFISKAEKALEEENYDDAAYDLLTVRRAAPKNKRFMKI
                     MKEPGVQRMVSTMEMNIMRDKKNLNEIDERIYYAIDEKDHSVTLSEMGLNELHPSDKQ
                     LFEIPDIAEAIVQIDNDEKLTPEDKVKAKDRVYKQHIEKSQKLHSITQLLKAYSLFEK
                     DVEYVVQDGKVIIVDEFTGRLMPGRRFSDGLHQAIEAKEKVKIEGETQTVATITLQNY
                     FRMYNKLAGMTGTAETEAGEFWDIYKLDVVVIPTDKPVKRIDFPDQIYRTKKEKYKAI
                     MDEIEYFQEIGRPVLVGTVSVDVSETISRMLKRKGIKHEILNAKQHQREAEIVQFAGR
                     QRAVTIATNMAGRGTDIKLGENVLKHKNCALIETPEYGEVCPYLEKYKCDETPACGLH
                     IIGTERHEARRIDRQLRGRAGRQGDPGSSRFFLSLEDDLMRLFGVDRISRVLDMLGLK
                     EGEAIEHKRISKSIEKAQKSVEGQNYGVRKRLLDYDDVMNIQREVIYDRRNEYIRTDN
                     IQEEVREKISEVAENMVTTYAPPEEDPAAWDLSGLAMETHKMFLAPLDTKNIDVMQIE
                     HEDLVKKVEELALAVYKQKEEVLGSEVMRKIEKMAFLSTVTEKWKDHLYEMDQLRTGI
                     GLRSYAQKDPLIEYKREAFRMFTELLDQIDREAVRFCYWMRPADQSEVSRRERESRAA
                     AKARHDKKESLGFRAPEGGAVQAQGQPGEDDTPQQGKKQPFKREGKKIGRNDPCPCGS
                     GKKYKKCCGRDV"
     gene            3415..>3922
                     /locus_tag="ENO07_00815"
     CDS             3415..>3922
                     /locus_tag="ENO07_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00534.1"
                     /translation="MITLIRIIIIVIAAIFALSWLYPAYWNWAVMPLEIIYPVNIALL
                     AIAVILTIFTGLSEIIWKLISRVWEAFLPKIASMPGTLKLSLWIIIPAIFFCLIRSQS
                     LILGDGDLILGNIVEGKLISSTAFGYSQLVSLLAELFGIENLDQAANLMAALSIVCGL
                     VYTYFLYKT"
BASE COUNT         1076 a          936 c         1060 g          850 t
ORIGIN      
        1 gcttttgttc ctggttccga ctttttatgt cttcaccaaa cggaatctaa cccccaagga
       61 ctgattcaag ctcgtttttt tcttgcaaat tatcaaaaag cgtataatga tatggatcga
      121 acgtttgcgg gaatattttg tataactgtg ttacccctgt aaatgcaaag aggagataat
      181 attgttagat actctgatta aaaaagtatt cggaacaaaa cacgaaagag acctgaaaaa
      241 gataaatccg attgttgatg agataaacga aatctatcct acgctcgagg atgtccctca
      301 ggaagagctg ataaaaagaa ccgaggaatt caaggccgag gccaaaaagg tctgggatga
      361 gcttgaacag caggccgagg aattcgaatg ggacaccaag gagcgtaaag agaaattcaa
      421 agagggcatc gacgaatacc tcgatacaat ccttcccgag gcctttgcga tggtcaagga
      481 agcctgccga cgcctgaagg gccaaacctg ggaggccggc ggaatgaact tcacctggga
      541 tatgattccc tacgatgtcc agctctttgg cggtattgtg ctgcaccagg gaaaaatcgc
      601 cgagatggcc accggtgagg gtaagaccct tgtcgccacc atgccggttt atcttaatgc
      661 cctggtcggg cggggagtgc atattatcac tgtcaacgat taccttgccc ggcgtgacgc
      721 tgaatggatg ggcgaggtct ataaatacct ggggctgacc gtcggcgtga tccagcatgg
      781 ccaggatgtc gacgagagac gtgcgcagta taactgcgat ataacctatg gcaccaattc
      841 cgaattcgga ttcgattacc tgcatgacaa catgggctgg cgtgtggagg ataatcgcca
      901 gcggggacat ttttatgcta tagtggacga ggtcgacagc gttttgatcg atgaggcccg
      961 taccccgctg attatctccg gcccggttga atccacaatc gaccaggcct tccgcgatat
     1021 gcagccgctg gtcgagcgca tcgccaaaaa gcagacactg atggccagcc agttcatcag
     1081 caaagcggaa aaggcgctgg aagaggaaaa ttatgatgac gccgcctacg atctcctgac
     1141 cgtcaggcgg gccgcaccga agaataaacg ctttatgaaa ataatgaagg agcccggggt
     1201 acagcgcatg gtctcgacca tggaaatgaa catcatgcgc gacaagaaga atttgaatga
     1261 gatcgatgag cgtatttact acgccatcga cgaaaaggat cattcggtaa cgctttccga
     1321 aatgggctta aatgagcttc atccctccga taaacagctc ttcgaaattc cggacatcgc
     1381 cgaggctatc gtccagatcg acaatgacga gaaactgacc cccgaggata aggttaaggc
     1441 caaggacagg gtttataagc agcatatcga aaaatcccag aaactgcatt ctatcaccca
     1501 gcttctgaaa gcctacagcc tgtttgagaa ggatgtcgaa tatgttgtgc aggatggtaa
     1561 ggtcatcatc gtcgacgaat tcaccgggcg cttgatgccc ggcaggcgtt tctcagacgg
     1621 tttgcatcag gctatcgagg ccaaggaaaa agtcaaaatc gagggtgaaa cccagaccgt
     1681 ggccaccatt acattacaga actacttccg catgtacaat aaactcgcag gaatgacagg
     1741 taccgcggag accgaggcgg gcgagttctg ggatatttac aagctggatg ttgtcgttat
     1801 cccgaccgat aaaccggtaa aaaggatcga ctttcctgac cagatctacc gcaccaagaa
     1861 ggaaaaatat aaagcgatca tggacgaaat tgaatatttc caggaaatcg gccgtccggt
     1921 gctggttggc actgtcagcg ttgatgtctc cgagacaatt tcgcgcatgc tcaaacgcaa
     1981 ggggatcaag cacgaaatct tgaacgccaa acagcatcaa cgtgaagcgg aaattgtgca
     2041 gtttgccggg aggcagagag cggttacgat tgctaccaac atggccggtc gaggcacaga
     2101 tatcaagctg ggcgagaatg tgctcaagca taaaaactgc gccctgatcg agactcccga
     2161 atacggcgag gtctgcccct atctcgagaa atataaatgc gacgagaccc ccgcctgcgg
     2221 gctgcatatt atcggaacag aacggcatga ggcccgccgt atcgaccgtc agctcagggg
     2281 acgtgcggga cgtcagggcg acccgggatc atccagattc ttcttatccc tcgaggatga
     2341 cctgatgcgt ctcttcggcg ttgacagaat atcccgtgtg ctcgacatgc tgggcctgaa
     2401 agagggcgag gcgattgaac acaagcgcat atccaaatca attgagaagg cgcagaagtc
     2461 tgtcgagggc cagaactatg gggtacgtaa acgcctgctg gattatgatg atgtcatgaa
     2521 tatccagcgt gaggtcattt acgatcgccg gaatgaatat atccggaccg ataatatcca
     2581 ggaagaggtc agggaaaaaa tttctgaagt tgccgagaac atggtcacga cctatgctcc
     2641 gcccgaggag gatccggccg catgggatct ttccggactt gcaatggaga cccataagat
     2701 gttcctggct ccgcttgata ctaaaaatat tgatgtgatg cagatcgagc atgaggatct
     2761 ggtcaagaag gtagaggagc ttgcattagc tgtctataag cagaaagaag aggtgctggg
     2821 ctccgaggta atgcgcaaga tcgagaagat ggctttcctt tccactgtga cagagaaatg
     2881 gaaagatcat ctttatgaga tggatcagct ccggaccggc atcgggctgc gctcctatgc
     2941 ccagaaggac ccgttgatcg agtacaagcg tgaggccttc cggatgttca cagaactgct
     3001 ggatcagatc gaccgcgagg cggtgcgttt ctgctactgg atgcgtccgg ctgaccagag
     3061 cgaagtctcc cggcgcgaac gggaatcccg ggcggcggca aaagcgcgtc acgacaaaaa
     3121 agaatcgctc ggtttccgtg ccccggaggg cggagcggtg caggcccagg gtcagccggg
     3181 cgaggatgac actccgcaac agggaaagaa acagcccttc aagcgagaag gcaagaagat
     3241 cggccgcaat gatccctgcc cctgtggctc cggcaaaaaa tacaaaaaat gctgcggccg
     3301 cgatgtttag ttaagcaggc aacctggaat aaatacgagt gtggccggga atcccttccc
     3361 ggccaaaata agcaggtcaa atcttgaaaa gatttgacat tttttacgaa aaagatgatt
     3421 acactcatcc gcataataat catcgttatt gccgcaatct tcgcactttc atggctgtat
     3481 cccgcctatt ggaactgggc ggtgatgcct ctggagatca tatacccggt caacatcgcc
     3541 cttctggcaa ttgccgtaat cctgaccatc tttaccgggc tcagcgaaat tatctggaag
     3601 ctgatatcga gagtgtggga ggcttttctg ccgaagatag catccatgcc aggcacactg
     3661 aaattatctc tctggataat aattccagca atcttcttct gtctgatcag gagccagagc
     3721 ctgattctgg gtgacggcga tctcatactt ggtaatatcg tggagggcaa gctgatatca
     3781 tccactgcct ttgggtacag ccagcttgtc agccttctgg cagaattatt tgggattgaa
     3841 aatctggatc aggcggcaaa cctgatggcc gcgctgtcga ttgtttgcgg gttggtgtac
     3901 acatacttcc tgtacaaaac cc
//
LOCUS       DSEB01000040            1604 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1016497, whole genome shotgun sequence.
ACCESSION   DSEB01000040 DSEB01000000
VERSION     DSEB01000040.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1016497"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1585)
                     /locus_tag="ENO07_00820"
     CDS             complement(<1..1585)
                     /locus_tag="ENO07_00820"
                     /inference="COORDINATES: protein
                     motif:HMM:PF07228.10,HMM:PF17203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HDS00535.1"
                     /translation="MIRKIMDDVDYRARRNENRLILVKNFPKRTAARRALRGEGISIR
                     KKFAFWMTLILTGLVFIIYFYFENRTTTALRSNFRTQMEEVATTVASQAKDEIVNEAR
                     DAEFLLMVRNFRHQNKNIAYISITDKDGEVLAHSEDISLLHSKYQLPPGIISELTTVP
                     QQVNSGGRDILHYILPVTENGELLGHAHIGFSTANLKADIRSSRQGIFIISIISYVLA
                     LMAVTLLSNYFVKPIQKLTDGVKKIGSGDLEAKLPIDGADEFSEIARAFNEMTGKIKE
                     AQKNIVEQERLHKEMQVAQEIQHTLLPKHFPDIEGYDIATIYRAAKDVGGDYFDFVWV
                     DENTLGIVVADVSGKGVPGSLVMTMIRTAIRLEARGNRSAVDILSRVNDFVTEDVKKG
                     MFITIFLVVLDSINRKISFASAGHNPMVLYRHDEEKTYFLNPRGIPLGIRLPEELTFG
                     ESLEAESIKLKKDDILVVYTDGITEAMNHRREQFGMTRFIQFIRNYSFLTPEDFVEKL
                     NEEIIEFTEGAEQNDDITLV"
BASE COUNT          373 a          429 c          388 g          414 t
ORIGIN      
        1 caaccagcgt gatatcgtca ttctgctcgg cgccctctgt aaattctatt atttcttcat
       61 tcagtttttc aacaaaatcc tcgggcgtca ggaagctgta attccggata aattgtatga
      121 accgggtcat tccgaactgc tcgcgccgat ggttcattgc ctccgtgatg ccgtccgtat
      181 ataccaccag gatatcatcc ttcttgagct tgatcgattc ggcttccaga gattccccga
      241 atgtcagctc ctcgggcagg cggataccta atggtatgcc gcgaggattc aggaaataag
      301 ttttttcctc atcatgccgg tataatacca tcggattatg ccccgctgat gcgaacgata
      361 ttttgcgatt gatcgaatcc agcacgacca ggaagatggt aatgaacatg cccttcttga
      421 catcctcggt tacaaagtcg ttaacgcgtg acagaatatc caccgccgaa cgattgccgc
      481 gcgcttcaag ccggattgcc gtacggatca tggtcataac cagggacccg ggcacaccct
      541 tgccggaaac atccgcgacc actatcccca gagtgttctc gtcgacccag acaaagtcga
      601 aatagtcgcc gccgacgtct ttagctgcac ggtagattgt ggcgatatca tatccttcga
      661 tatcggggaa atgcttgggc agaagcgtgt gctggatttc ctgggccacc tgcatctcct
      721 tgtggagacg ttcctgctcg actatatttt tctgagcttc tttgatcttc ccagtcatct
      781 cattgaatgc acgcgcgatt tcagaaaact catccgcccc gtcgatcggc agcttggcct
      841 ccaggtcacc ggagcctatc ttcttgacgc catcagtcaa tttctgaatc ggtttcacga
      901 agtaattgga cagcagggta accgccatga gggccagcac ataacttatg atggaaatga
      961 tgaaaatgcc ctgccgggaa ctgcgtatat ccgctttcag gttggcggtc gagaaaccga
     1021 tgtgcgcgtg tcccaggagt tccccatttt cggtaacagg cagtatataa tgcaggatat
     1081 cccgtccgcc gctgttaacc tgctggggaa cagttgtaag ttcggaaatt ataccggggg
     1141 gcaattgata ttttgaatgc agcagtgaaa tatcctcgct gtgtgccagc acttcaccat
     1201 ctttgtcggt gatagagata taggcaatat tcttgttctg gtgccgaaag ttcctgacca
     1261 tcaaaagaaa ctccgcatca cgcgcctcgt tgacgatctc atccttggcc tgcgaggcca
     1321 ctgtggtggc gacctcctcc atctgggtcc gaaaattgct cctcaaggct gtggtagttc
     1381 ggttttcgaa atagaagtaa atgatgaaca ccagcccggt caggatcaat gtcatccaga
     1441 aagcgaactt cttgcggatc gagatgccct ctcctcttaa ggctcgacgc gcggctgtac
     1501 gcttggggaa atttttgacc aggatgagac gattctcatt gcgccgggcg cgataatcga
     1561 catcgtccat aatcttccgg atcaaatata tgcccagccc cccc
//
LOCUS       DSEB01000041            1795 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1012843, whole genome shotgun sequence.
ACCESSION   DSEB01000041 DSEB01000000
VERSION     DSEB01000041.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1012843"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..419
                     /locus_tag="ENO07_00825"
     CDS             <1..419
                     /locus_tag="ENO07_00825"
                     /inference="COORDINATES: protein motif:HMM:PF02518.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDS00536.1"
                     /translation="SILRQVRTDFARDNIEISFEPADKEYPVLDDGIGFRQAFLNLII
                     NARDTYKDGGQIRIELTSNGDEHRLQIVDRGGGIKKELKKRLFEPFSTTKAQGSGLGL
                     YVAKKLIEQNGGRLDLRNNNEGGATAEIILPAYKEQ"
     gene            419..1750
                     /locus_tag="ENO07_00830"
     CDS             419..1750
                     /locus_tag="ENO07_00830"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00072.22,HMM:PF02954.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDS00537.1"
                     /translation="MAKILIVDDEEKMTHLVSGELEDNGYRCDIANSGQAALEKFRSN
                     IYDIVITDLRMDPPDGLALLRKIKAENSQTEVIIMTAYATASTAVEAMKSGAYDYIIK
                     PFSLDELVLKVEKILDEKRRSDRLEQLEEDLKSRSYDTFIGESKPVKRLFELVEKVSR
                     TDANVLLLGKSGTGKELVANMIHSSSPRRERPFVAVNCAALTETLLESELFGHEKGAF
                     TGAIKRKPGRFELAEGGTLFLDEIGEMSGQLQAKLLRALEERKITRVGGTEEIDIDVR
                     VISATNRDLEADMEGGEFREDLYFRLNVFPIRIPSLSERVDDIPLLANYFARKSGYGH
                     GDLAPEVLDLLKEYNWRGNVRELKNIIERALILSDGDRIRPEHIAVGPAKQPERLHLR
                     AGSPEEGLDEMEKAAVIDALQKARGNKTRAAEILKITRRMLYTRLKKYGLD"
BASE COUNT          480 a          397 c          522 g          396 t
ORIGIN      
        1 agagtatcct gcggcaggtg cgtaccgatt ttgccagaga taatattgag ataagctttg
       61 aaccggcgga taaggaatat ccggtgcttg acgacggtat tggattcagg caagcatttt
      121 tgaatctcat tatcaatgcc agggacacat ataaagatgg cggtcagatt cggatcgaac
      181 tcacgtcaaa cggagacgag catagactgc agatagttga ccggggcgga ggcataaaga
      241 aagagctgaa aaaacgtctg tttgagccgt tttccacgac taaagcacag ggctccggcc
      301 tggggctgta tgtggccaaa aaacttatcg aacagaacgg gggccgtctt gatctacgga
      361 ataataatga gggcggagca accgctgaaa tcatcttacc ggcatataag gagcaataat
      421 ggcaaagatt ctcattgtag atgatgaaga gaagatgaca catctggttt ccggcgaact
      481 ggaagataat ggttacagat gtgatatagc caacagcggt caggctgcgc tggagaaatt
      541 cagatcgaat atatatgaca tagtgataac tgatcttcgc atggatccgc ccgatgggct
      601 ggccctgttg aggaaaatca aggctgaaaa ttcgcagact gaagttatta tcatgaccgc
      661 ttatgccacc gcatctacag cggtcgaggc gatgaagtcc ggcgcctatg actatatcat
      721 caagcccttc tctctcgatg agcttgtact caaagtggaa aagatcctgg atgaaaaacg
      781 ccgcagtgac cgcctggaac agcttgagga ggacctgaaa tccagaagct acgatacatt
      841 catcggtgaa agcaagccgg tcaaacgcct cttcgagctg gtggagaagg tcagccgaac
      901 tgacgccaat gtgctccttc tgggcaagtc gggcacaggt aaagagctgg tagcaaatat
      961 gatacacagc tcctccccgc gccgggagag gccctttgtg gcggtcaact gtgccgcttt
     1021 gaccgagacg cttctggaat cggaactttt cgggcacgaa aaaggcgctt ttacgggcgc
     1081 tattaagcgt aaaccgggac gtttcgaact ggccgaagga ggcacactgt tcctggatga
     1141 gatcggcgag atgtccgggc agcttcaggc caaactcctg agggctttgg aggagaggaa
     1201 aataacacgg gtgggcggca ctgaagagat cgacattgat gtgcgcgtta tttcggccac
     1261 aaatcgcgat ctggaggcgg atatggaggg gggcgagttt cgcgaggatt tgtatttccg
     1321 tctcaatgtc ttccccatca gaattccctc attgtccgag agggttgacg atatcccttt
     1381 gctggccaat tattttgcgc gaaaaagcgg ctatgggcat ggcgatctgg ccccggaggt
     1441 gctggatttg ctcaaggaat ataactggcg ggggaatgtg cgggaactga aaaatattat
     1501 tgagagggcc ctgattcttt ccgatggcga tagaatccgg cctgagcata tagctgttgg
     1561 ccccgcgaag cagccggaac ggttgcattt gagggctggc tcgcctgagg aaggtctgga
     1621 tgagatggag aaggctgcgg tcatcgatgc tctgcaaaag gcccgtggaa ataagaccag
     1681 ggcagcggag atcctcaaga tcacccgccg catgctctac accaggctca aaaaatatgg
     1741 acttgactga tggaaatgta ccgtttggtg agccattttg taccttaacg tacag
//
LOCUS       DSEB01000042            1573 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1017252, whole genome shotgun sequence.
ACCESSION   DSEB01000042 DSEB01000000
VERSION     DSEB01000042.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1017252"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(224..667)
                     /gene="tsaE"
                     /locus_tag="ENO07_00835"
     CDS             complement(224..667)
                     /gene="tsaE"
                     /locus_tag="ENO07_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDS00538.1"
                     /translation="MEPISFLSDSEQKTQELAEDFARELQKGSLVALTGQLGAGKTVF
                     ARGLCRGLGFEGIVSSPSYTLVNIYKGRFRINHVDLYRIENTDEIADLDPDELFYPEG
                     VTIIEWAEKIKQMLPARKWIIYLELAGKNKRRIEIFDSVWPDKIG"
     gene            complement(671..1573)
                     /locus_tag="ENO07_00840"
     CDS             complement(671..1573)
                     /locus_tag="ENO07_00840"
                     /inference="COORDINATES: protein motif:HMM:PF08665.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HDS00539.1"
                     /translation="MLPHLRRKQKVYFIVIDCMRYDQWLTIEPIIADYFQVEKDYYFS
                     ILPTATPFARNAIFSGEYPDKVAEILPEMWKDAARTESSLNANEKELLERQLKQKVID
                     LKGELRYAKVLEQREAENLARRISTYQNVGLMAMVFNFLDLMIHGRSESEILLEIAPD
                     ESAFRSLMRSWFMHSSLFTILKYLSTVDCKVVITTDHGSILANKETTVKGRKEASTNL
                     RYKYGDNLNCNPREVMLVKNPEDIRLPRFGVSTTYIFAKEDYYLVYPTQYHHYVSKYA
                     NTFQHGGISLEELILPVITLTPRA"
BASE COUNT          394 a          371 c          370 g          438 t
ORIGIN      
        1 gtattaatcg ccagaagatc cacatcagcg agttttctat cggtatctgc gaagagtcta
       61 tctatctgtg atatgatctt ttcagaatga tttcccatgc tgtctgatag caactgcttg
      121 agactctgat cgttttggaa aagcgcggtc ttgagaattc gctctgagct gtccaggcat
      181 aaaatcaaca tgggtctaaa attaatactt cagggattgg caatcaacct attttatccg
      241 gccagaccga atcaaatatc tcgatccgcc ttttattttt gccggctaat tccagatata
      301 taatccattt ccgggcggga agcatctgct taattttttc cgcccattcg attattgtaa
      361 ctccctcggg ataaaaaagt tcatccgggt cgaggtcggc gatttcatcg gtattttcga
      421 ttcggtacag atcaacatgg tttatcctga atctgccctt ataaatattg accagcgtat
      481 aggagggtga actgacgatt ccctcgaatc ccaacccccg gcacagcccc cgcgcaaaga
      541 ctgtcttgcc tgcgcccaac tggcccgtca gcgcaaccag agagcctttc tgcagctccc
      601 gggcgaaatc ctctgcaagc tcctgagttt tttgctccga atccgacaaa aatgaaatcg
      661 gctccataca tcaggccctg ggggtgaggg ttatgactgg cagaatcagt tcttccagag
      721 atatgccgcc atgctggaat gtgttggcat atttggagac atagtgatgg tactgggttg
      781 ggtataccag gtagtaatct tccttggcaa aaatgtaggt ggtggaaaca ccaaaacgag
      841 gcaggcggat atcctccgga tttttcacaa gcatgacctc gcgcggattg cagttgagat
      901 tatccccgta cttatagcgc agattggtgg aggcttcctt gcgtcccttg accgtggtct
      961 ctttatttgc aagaatcgag ccatggtcgg ttgtaattac gactttgcag tcgactgtgg
     1021 aaagatattt gagaatggta aagagcgacg aatgcataaa ccaggagcgc atcaacgacc
     1081 tgaaggccga ctcgtccggg gcaatctcga gcaggatttc cgattccgaa cggccgtgaa
     1141 tcatgaggtc cagaaaattg aagaccatag ccataaggcc cacattctgg tacgtggata
     1201 ttcgcctggc aaggttctca gcttctcttt gctccagaac ttttgcatat ctcaattctc
     1261 ccttgaggtc gataactttc tgcttgagtt gacgttccag gagctccttt tcgttagcat
     1321 tgagtgatga ttctgtcctg gccgcatctt tccacatctc cggcaatatt tcggcaactt
     1381 tatcgggata ttccccggaa aatatggcat tacgcgcaaa aggcgtagcg gtggggagga
     1441 ttgagaaata ataatctttc tcaacctgaa aatagtctgc aattatcggt tctatggtca
     1501 gccactggtc ataacgcatg cagtctatta cgataaaata tactttttgc tttctacgca
     1561 gatggggaag cac
//
LOCUS       DSEB01000043            2378 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1006900, whole genome shotgun sequence.
ACCESSION   DSEB01000043 DSEB01000000
VERSION     DSEB01000043.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1006900"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..819
                     /locus_tag="ENO07_00845"
     CDS             <1..819
                     /locus_tag="ENO07_00845"
                     /inference="COORDINATES: protein motif:HMM:PF00730.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HDS00540.1"
                     /translation="RYYRAIPYHGKICPVEVKSVGEIEKPRILARSLAGSPEKYEQNV
                     IAQNLKTIFRIGYSLHGFYEFCKTDHILKRLSERYYGLINLQTVDPFEILVWAITGQQ
                     MSLKFAFRLKRRLVEKYGKRFQVDGKTIYTFPKPEVLAGAKTSDLLKMQYSRNKADYI
                     KSLAASVTDRKIDLEKLRGFDDNTVRDILTGIRGIGGWSCEYAMLRSLGRDDACPSAD
                     AGLRRALAENYGIAQKSSEKDVDNFMKRFMPFRGMATYYLWFGLLEKSTKDGVP"
     gene            816..1331
                     /locus_tag="ENO07_00850"
     CDS             816..1331
                     /locus_tag="ENO07_00850"
                     /inference="COORDINATES: protein motif:HMM:PF01035.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDS00541.1"
                     /translation="MKKIYFTEFDSGKWKIYLAASDKGVVKISYGASSKFDFFAWLED
                     KFPGYMFEKNDSRTSAYASNIKKYINGKIKKLDIPIDLQANGFTKKVLTKLKKVPYGK
                     TVTYGELAAMAGNPRASRAAGTACSKNPIPLVIPCHRVIAAGGRIGKYGGGERLKRKL
                     LENEGVLGLKD"
     gene            1381..>2378
                     /locus_tag="ENO07_00855"
     CDS             1381..>2378
                     /locus_tag="ENO07_00855"
                     /inference="COORDINATES: protein motif:HMM:PF02073.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDS00542.1"
                     /translation="MDPRIEKLADVMVNYSLELKQGQWVKVQGINTAMPLIKAFFKKA
                     LDAGAHPYYHAMVDDIEEIFMKYGSDEQLTFISDIQKIETEKVDAMFAIMARDNSKYL
                     SNVDPDRQALAQGARKDLFTKFMERSANGDLKWVGTMYPTISAAQDADMSLSEYEDFV
                     YEAGHLNDDDPVVFWKSMSKTQDKQAEYLQGFREIHVKSKDTDLKLKVEGRKWVNCDG
                     KYNFPDGEIFTTPIEDSANGHIVYTYPACYGGREVANVRLEFKEGKLVDFSADKNQDY
                     LEKMVNMDEGARILGEFAIGTNYNIDKFTKNILFDEKIGGTIHLALGAAPPETGGT"
BASE COUNT          683 a          483 c          642 g          570 t
ORIGIN      
        1 cgctattacc gtgcaatacc gtatcatgga aaaatctgtc cggtcgaggt taagtcggta
       61 ggagagatcg aaaagcctcg gattttggca agatccctgg ctggttcgcc cgagaaatat
      121 gagcaaaatg tgattgcgca aaatctaaaa accatatttc gtatcggcta tagtttgcat
      181 ggtttctatg agttctgcaa aacggatcac attttaaaaa gactttcaga aaggtattat
      241 ggtctgataa atctccagac tgtcgatcca tttgaaattc ttgtctgggc cataaccggg
      301 caacagatga gtttgaaatt tgcctttaga ttaaagcgca ggctggtgga gaaatacgga
      361 aagagatttc aggttgacgg aaagacgatc tatacatttc caaaaccgga agtcctggcc
      421 ggggcaaaaa catcagatct tctaaagatg caatattctc gaaacaaggc tgattacatc
      481 aaatcccttg cggcttctgt tacggacaga aaaattgacc ttgaaaaatt gcgcggattt
      541 gatgataata cggttcgcga tattctaacc gggataaggg gaataggagg gtggagctgt
      601 gagtacgcca tgctgcgctc gcttggacgg gatgatgcct gcccctcggc tgatgccgga
      661 ttgagaaggg cgctggcgga aaattacggc attgcgcaaa aaagcagtga aaaagatgtt
      721 gataatttta tgaagagatt catgcccttc cggggtatgg caacctacta cctctggttc
      781 gggcttttgg aaaaatctac aaaggatgga gtaccatgaa aaaaatatat ttcacagaat
      841 ttgacagcgg caaatggaaa atttatctgg ctgccagcga taagggagtg gtgaagattt
      901 cctatggcgc aagttcgaag ttcgatttct tcgcctggct cgaggataaa ttccccggct
      961 atatgttcga aaaaaatgat tccaggacat cagcttacgc cagtaacata aagaagtaca
     1021 ttaatggtaa aatcaagaag ctcgatatcc ccatcgacct ccaggccaat ggttttacca
     1081 aaaaagtcct gacgaaattg aagaaagtac cctatggcaa gactgtaacc tatggcgaac
     1141 tggcggccat ggccggtaat ccgcgcgcca gccgtgcggc cggaacagcc tgctcaaaaa
     1201 atccgattcc gctggtgatc ccatgccatc gggtaatcgc cgccggagga cgaatcggca
     1261 agtacggcgg gggagagcgt ctgaagagaa agctccttga aaacgagggc gtgcttgggc
     1321 tgaaagacta atgagctaat gatttgggaa tgatcttatt cacatagaaa ggaaaatctg
     1381 atggatccca ggattgagaa acttgcggat gttatggtta attattcact ggagttgaaa
     1441 cagggccaat gggtgaaggt gcagggtatc aataccgcca tgccgctgat caaggccttt
     1501 tttaagaagg cgctcgatgc cggcgctcat ccgtattatc atgccatggt cgatgatatc
     1561 gaagagatat tcatgaaata tggttcggat gagcagctca catttatttc tgacatacag
     1621 aaaatcgaga ccgagaaggt cgatgcgatg tttgctatta tggcgcgcga caattccaaa
     1681 tatctgagca atgtcgatcc cgatcgccag gcgctggcgc agggagcgcg caaggatctg
     1741 tttacgaagt ttatggaaag gtcggctaat ggcgatctga aatgggtcgg cacgatgtat
     1801 ccgacaatct cggcggcgca ggatgccgat atgtcgcttt cggagtatga agatttcgtg
     1861 tatgaggccg ggcatctcaa tgatgatgat ccggtagtct tctggaaatc gatgtcgaag
     1921 acccaggata aacaggccga gtatcttcag ggattcaggg agattcatgt caagtctaag
     1981 gatactgatc tgaagctcaa ggtcgaggga cgcaaatggg tcaattgcga cggcaagtac
     2041 aatttcccgg acggcgagat cttcaccaca cccatagagg attcggcgaa tggtcatatt
     2101 gtctatacgt atccggcctg ctatggcgga agagaggtgg ccaatgttcg tctggaattc
     2161 aaagagggaa agctggtcga tttctcggcc gacaaaaacc aggattatct cgagaagatg
     2221 gtgaatatgg atgagggtgc gcggatactg ggtgagtttg cgatcggtac caattacaat
     2281 atcgataaat tcacaaagaa catattgttc gatgagaaga tcggcggtac gattcatctg
     2341 gctctgggtg cggctcctcc cgagaccggc ggtaccaa
//
LOCUS       DSEB01000044            1993 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1010181, whole genome shotgun sequence.
ACCESSION   DSEB01000044 DSEB01000000
VERSION     DSEB01000044.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1010181"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..350
                     /locus_tag="ENO07_00860"
     CDS             <1..350
                     /locus_tag="ENO07_00860"
                     /inference="COORDINATES: protein motif:HMM:PF12867.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HDS00543.1"
                     /translation="YYLSGQDDDFKPPEPFTLSELDPSGVMPDRVYTKDELLTYLKHC
                     HEKARKVIKEMTEQWIEQQYKFAKVTLNYGELLLYNMRHFQHHAAQLNMILRRETDSA
                     PGWVFTAKKPLGL"
BASE COUNT          490 a          538 c          467 g          498 t
ORIGIN      
        1 actattatct gtcgggccag gatgatgatt tcaaaccacc ggagccattc acattaagtg
       61 agcttgaccc ctctggtgta atgcccgatc gggtctacac caaagatgaa ctcctgacct
      121 atcttaaaca ttgccatgag aaggctcgga aggtaattaa ggagatgacc gagcaatgga
      181 tcgagcagca atataaattt gcgaaagtca ctcttaatta cggtgagctg cttctataca
      241 atatgcgtca tttccagcat catgccgcgc agcttaatat gatcctgaga cgggaaaccg
      301 attcagctcc cggctgggtc tttactgcga agaagccatt ggggttatga ccatatttag
      361 atagacttca gccccatcat atttttcctc caatatgtgt ctgagactac acgggctttt
      421 aacttaatga tttgatcgca ttttttttga ctttgacaaa gaaaaggaaa cctgaatttc
      481 tccggtacaa ctgccagaag agctcgatct caggcggtta tacataatct ctccctctac
      541 aaccgtgaac ttgtcagctc aacatcagcg cgggaccagg cttcctgaaa tctatcatat
      601 actttttgag cctcaaaatc cttcccctgt tctttaaggg cctgatacag tccgaacaat
      661 gaccagccgt tatcgggcca ttcttccaga tcacgacgat atacctgctc ggcctcctga
      721 gcatttccag cttcaagcaa gattgccccg agggtctgac ggaccggggc ataccaggcc
      781 ggaggttcat gataaccgag gctgtgctcc agcctgaccg cctcacgcaa gagcttaatc
      841 gcatctgcat aattactttc ggcggcccaa atttcggcat ttaaaacatt gatcgcaatc
      901 tgcatgacat ttccggtagt attaacgccc cagactccca tcgcctgaag tgtctcccga
      961 ttattcattc ctttgagggc atcgagttct gcctgggcac tctgcaaatc gttgctgcgg
     1021 acatatgcaa gcccgcgtgc ataatgccat atggcaaccg gaaaaagtaa ttcatcatca
     1081 ggcttcggct cgtccaggat catgtcccac atcccgaaac gtaccatggc caggtacggc
     1141 agagcgtaat gctcataaag tgaaccatag ccgggctggt acaaaaggtc ctcgatgata
     1201 tagtctttcg tgatgttggc cgcttcgagg catttctgac cccgcccctc cattgcggtg
     1261 gtgaaccaca ggaaatgtga gttgtgcggc atataagaga tacgatacat accatgcgcg
     1321 ccatgctgat tcaagtagcg ctgatcggcc tcgacagcct tttgatttgc ctctgagccg
     1381 tcagcatatc ttccaatgtg gatatagatg tgcgagggca tatgcagaag atgaccgata
     1441 cctggtacaa gcccgcgcaa cctgtcagcg ctggcaaccc cccacttggg atgttccatt
     1501 tcaaccaggt gaatgtaaaa atggtttaag cccgggtgct gcggattatt ctgcaggccg
     1561 gtctccaagg ttgaaagaac ttcacgggct atgctgttcg gttcaccatt atccagccag
     1621 taattccagg gcgtggtgac catcaaggcc tcggcaaaca gcgcctgagc gtcgggatca
     1681 tcaggatatt tgctcgccag ttccctcatc gcatcaacat aatcccggtc caattccgag
     1741 cggtcggcgg gaggattctc cgcatagcgg cttgccagag catcaatata gtccttttct
     1801 ttttcggaga tgtcccctga tagctcctgc gctctttgaa tagcatccca gacgacctgc
     1861 acacttgcct cggacatgcc ggcgttaata tttgggccga atgtcagggc ctcgccccaa
     1921 tatgccatcg cgcagtcagg atcaagacgt tgggcctccc gaaatgaacg cgccgcctca
     1981 gcgtgattga aac
//
LOCUS       DSEB01000045           14404 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000132, whole genome shotgun sequence.
ACCESSION   DSEB01000045 DSEB01000000
VERSION     DSEB01000045.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14404)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14404)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14404
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000132"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(132..2198)
                     /locus_tag="ENO07_00865"
     CDS             complement(132..2198)
                     /locus_tag="ENO07_00865"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00072.22,HMM:PF13185.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDS00544.1"
                     /translation="MTTKANNSTYMAEIEDMIVPGGAENQTISHIIYNQFKKNLESGY
                     FIMPDDSPYAEIFEDMDFSPPLQECTFVSRTEYHELIRRVGLHENAFFLPYEDFDLAL
                     KLKFMPGFPESALGADNLIPLNNYIRTYLGKIIKAYTVYLNVLNHLSQMMLTSVEIET
                     IFPVLVKELKKLVNFSHMSLVTYHPDFESFRLEAEYDDQNDACSFKKQFIKLSETSLA
                     GLLSKKKAFIASLKDIGQGVVAQRMFRMGYQSFLFSPIYDKGELLASLNLATRDVDAY
                     DDRHIKLLSKVCGVTGGALKSAVNHSHLQQLMNDYDNARKHFMQLEKSRNFIDITRGV
                     LHAFNNHLALIMGRAQILRQFSGDVIKLETARKGLDIILNASTQASNQIAELQKYARL
                     RPDDDPDLIQFKGLIEEIIELSMPRWKAIAHGVIEFRLNIDTQLVIKGYRKKIKEALI
                     NILMNAVEAEEEHGGEISISARAEGDMAVICIKDQGKGIEKDAIPRVFNPFYTTKPDG
                     TGLGLAVSYRIIKEHSGRLEIESSPGKGTAVTITLPLKIRKTESSFGNSDSRLIKNIV
                     VVDQNRIRRNLLSNLLQNLKLKVKSAKSLASVDGIIAEEKPDLVILDDELEEVNPVEY
                     FAMIKTRLPEIRTCLVSVSARPDEQKRLKEQGVDAILTKPFEQGQIRETIDRLCRTAP
                     SKQPSE"
     gene            complement(2195..4246)
                     /locus_tag="ENO07_00870"
     CDS             complement(2195..4246)
                     /locus_tag="ENO07_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDS00545.1"
                     /translation="MTKISWAIITILLLALLWLWKRRKAALLPDLSSGYSRIVSSLVL
                     LIGWSALNLLQNIPNIESILGTTVLILLDSFSTALLPGAILLLVITFLIDFALIRNHG
                     AFRKRSVSFAGLMGEIAGVSVNRGKLAFVEEASAVLAKILSADYYQITKIENDQRVNL
                     GACSIQHGLDVKNGHRFRMPDDGLNSSDREPLCRRHKQICLCLKSNLPEAYELIFLLP
                     KYQRILKKPEFQDILRSVLISKLENAAQNEELKERLDIESRQNRLYLENDRDSDIAAY
                     LQRIYPILSEKVKFDYISVAVLDSAIQNMYRYTYADVGGSLIERGICYPLKQTAALRA
                     AGEARMLVANTLESDFYRDDYHLYKSGFSSRLVYPVRNDSGRVLAVLTLAAVKPKTFN
                     HLDEPDLQFLNAPLCRMIEFNNVSSMMNTLKKQVVSSFNLTFGLENSPGSKGFYDRSA
                     QILAETLPATMCRVWGYDQSLNRLRSIGYHSLHRRTDQISDSRKDVELELMPRHKAAI
                     KMGKAIIVNQSNPESNMGKSEVEFMGLPGIKSAILAPMRLQNRISGIVSVGEVRNWER
                     RSLGQQELLYSQIVSTISALVHEIDSKALELNRVNAVLNEIDKRSLVYETYTDLPSRI
                     SSKLSGIMGAAELIRRKMPEDAREMIRYNDIIIKGAETIAKELNRFGELKKSIFRDKA
                     V"
     gene            complement(4367..5017)
                     /gene="rnhC"
                     /locus_tag="ENO07_00875"
     CDS             complement(4367..5017)
                     /gene="rnhC"
                     /locus_tag="ENO07_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDS00546.1"
                     /translation="MEIIEAPDEYIGTDESGKGDYFGPLVIAGVYIGPGNQAHLEKLN
                     ILESKRISDNRAISLSEEIKKLSHTSVVVIGPEKYNELHDKMKNLNKLLAWGHARVIE
                     NILDLHDCRAAISDQFGDESLIKNSLMKKGKNIKLQQTPRAERFLAVAAASVLARGAF
                     LNYLNRLSSQYGIKFLKGASNLVDQTGRQFAEKFGHDKLKLVAKMHFKNTKRIEAP"
     gene            complement(5018..5575)
                     /gene="efp"
                     /locus_tag="ENO07_00880"
     CDS             complement(5018..5575)
                     /gene="efp"
                     /locus_tag="ENO07_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDS00547.1"
                     /translation="MIDTSDFFKGMKIEIEGEPFIILDFQNARTAQRRANVTTKLKHL
                     VTGQVLEKTFSSGTKFEEPPFETRDMQYMYNDGESYHFMDTDTYDQIAIPVDAIGDDK
                     WYLLENHTYEILFYRGNAININLPTSVVLEVVEAEPAIKGDRVSNVMKGAKVETGLEV
                     KVPIFVKEGDKIKIDTRDGKYLERM"
     gene            5943..6362
                     /locus_tag="ENO07_00885"
     CDS             5943..6362
                     /locus_tag="ENO07_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HDS00548.1"
                     /translation="MERKIRVLLAKPGLDGHDRGIKVIASALRDAGMEVIYTGLRQTP
                     AMIVEAAIQEDVDCVGISILSGAHMTLFPAIKQKLEEKGAEDIILFGGGIIPGDDIKA
                     LEAKGIDKLFGPGTPTEDIIKFIENAIAGRDKKEEIL"
     gene            6385..7170
                     /locus_tag="ENO07_00890"
     CDS             6385..7170
                     /locus_tag="ENO07_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00549.1"
                     /translation="MKPLNSKLLIASVFTFIALMVIPSTVMAQEYGKREAEEIWLTEN
                     DVDTAYEIVKLLSVKNTGTPKECRRENLEDLSKIASKMDAHAVVYITHNTPAGEKDAL
                     ITNGVAVKYLSPKEIEKRNKKAKKPPKLEKESPLDPEPLLIEYKDVDFPYKILGILEI
                     HTGPAETNFSSQAMDFRLIDDASRYGADGLIWVDYQRVGSDVTAALGIMIKAYNDWET
                     VEKIKAKEVEMMQARQKAAEERAKEQEKAEEPEVPDEPSETAE"
     gene            7231..8382
                     /locus_tag="ENO07_00895"
     CDS             7231..8382
                     /locus_tag="ENO07_00895"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00441.22,HMM:PF02771.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDS00550.1"
                     /translation="MTYNVDPELQQPIRDMMREFCPKEIEPLGLKHDREPDAFAHDIF
                     KKLADKGLVGYVMPKEYGGQGRKKIEYATMIEELSYFDPPSSLLSAVSQLAVDPIMNF
                     GTDEQKKKYIPKAATGELIPAFALTEPNAGSDAANQSTEFEDAGDHYVINGEKIFIMH
                     GDVCDYAVTFGKLKGDEGDRPKVSAIIVPSDAEGFEGSTLKHKMGMKYATTGRIIYKN
                     VKVPKENFLGPAGKGFKMAMMTLDGARVGIAAQSTGIAQRALDESIKYAKERVAFGAP
                     IAKLQAIQWMIADMATKVEAARLLTYKAAQLQDKGESINLSASQAKLFASEAANFCVD
                     RCMQIHSGYGYIGEFSIAEKLYRDQRVCEIYEGTSEVQRLVIANNMLRK"
     gene            complement(8627..9082)
                     /locus_tag="ENO07_00900"
     CDS             complement(8627..9082)
                     /locus_tag="ENO07_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00551.1"
                     /translation="MKEYRIRKAWWISYNLAGIVLIAMGVWAISLFFRDMVDYHRLIA
                     GIVIIVFVGYIVIDRNLLKIRLTDNYVARIGIRAVCIKFPDATELFLKNGKAFVKSEK
                     SSITISDEITGREEIIHFLTKKAVEYEIPISGDDYMLDKYWKPLKGDGK"
     gene            complement(9203..10177)
                     /locus_tag="ENO07_00905"
     CDS             complement(9203..10177)
                     /locus_tag="ENO07_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00552.1"
                     /translation="MTKQTFAINILTIALFVFPMLLHAEKPFKIVYADVETAKEILIT
                     RDEFINAMSPIDRSIRMKTTETVSVDSFVNFLGQNALEWNDAEIEILKTAMDSILPVL
                     QEIKLDFPDTVYFAKTTGEEEGGGVYTRQNAIIFPGVSEEVQNADLIHLIYHELFHIL
                     TRNNPEYQDDLYQIIEFYPCNEIEFPDTLKNRRITNPDAPHNNYYIEVEVEGEEIPVV
                     PIVVSSVRMHQVTEETNLIQSLSFGLMALENHDGMYVPKMKDGLPVLYYYTNVPSFME
                     KVGHNTEYVLHPEEILAENFVLIFSGDHDIPDPEIPQKMREILFGERN"
     gene            complement(10451..11008)
                     /locus_tag="ENO07_00910"
     CDS             complement(10451..11008)
                     /locus_tag="ENO07_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00553.1"
                     /translation="MAFRDYIVVIIQIIAVIAPFIVAWYAYHLGAKKERRQRELKKWR
                     LLDVIISDLSLRRKHLERLNEKIKEKEYKPVGLRIIEKSILSEFYQLIVSSPGFRKIY
                     ESIEYVYSSLEQLNGYILQLAQVKAKGIQVTDNMIEFRQYSFHLEDTNNRFENLKSQL
                     ESLAQTHDLTKFEGIDVELEEKMQE"
     gene            complement(11111..12661)
                     /locus_tag="ENO07_00915"
     CDS             complement(11111..12661)
                     /locus_tag="ENO07_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HDS00554.1"
                     /translation="MSIDKKLEQLKKLQDDARLGGGEKRIEAQHQKGKLTARERLDLL
                     LDKGSFEEFDMFVRHRSSDFGLDKQKYFTDGVVTGCGTIDDRKVFVFSQDFTIFGGSL
                     SKAHAEKICKIMDMAMKVGAPVIGLNDSGGARIQEGVISLGGYADIFLRNTLASGVVP
                     QISVVLGPCAGGAVYSPAITDFVLMVKGTSYMFVTGPNVVKTVTHEEITHDELGGAMV
                     HASKSGVAHLVGNNEAETLLMTRKLMSFIPQNNLDEVPMIESNDPDDRMDEELNYIVP
                     DNPNKPYDIKDVITRIVDNGDFFEIMPEYAMNIVVGFARLGGRSIGIIANQPAYLAGV
                     LDINSSDKAGRFIRFCDAFNIPILTFEDVPGFLPGTDQEFNGIIRHGAKLLYAYCEAT
                     VPKITVITRKAYGGAYDVMNSKHVRGDMNYAWPTAEIAVMGPKGAVEIIFKKEIAASD
                     NPEAATDKKVEEFREKFANPYIAAERGYLDDVIEPRTTRPRLIRAFDMLSTKRDQNPP
                     KKHGNIPL"
     gene            complement(12691..13530)
                     /locus_tag="ENO07_00920"
     CDS             complement(12691..13530)
                     /locus_tag="ENO07_00920"
                     /inference="COORDINATES: protein motif:HMM:PF00753.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDS00555.1"
                     /translation="MKLGKFQIDVIVENTAYVDGGMMFGVVPKKLWSKDVQVNEQNLV
                     PMDLNVFVIRTGEMILLVDTGLGEMLSERQQKIYGTAPESRLESGLAELGIKPDEVDY
                     VIHTHLHLDHVGGAVKDDGDGNPVPRFSRAQHILQKLEWEAANDPDERSEAAYDKSKF
                     MPLYDAGLLKIIEGNVELIPGIKLVLTGGHTMGHQGVIVESEGERLFLFADIVPFQSH
                     IRPAYVASADLFPLETIKIKKELLPDIAQHGWYLGFDHEIDIKVCRIIDTGMKLVAEK
                     VEV"
     gene            complement(13539..>14404)
                     /locus_tag="ENO07_00925"
     CDS             complement(13539..>14404)
                     /locus_tag="ENO07_00925"
                     /inference="COORDINATES: protein
                     motif:HMM:PF03129.18,HMM:PF13393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDS00556.1"
                     /translation="RVLDKLDKQGREAVTAELGPGRVDKSGDEIPGLNLKPEQISRIE
                     HFLDIPREDFEGTFEKCMQILGEYEIAKRGLDELKEIHEYLMASSVGMEYCVFDTTVV
                     RGLGYYTGPIFETILTDLPEFGSVFAGGRYDNLVGRFSGQEAPGTGASIGVDRLLAAL
                     KKLDLIEAQKTSTDVLVATMDRERMSDYFKIASELREQGWNVEVFLGNNRRIPKQLQY
                     ADRLDIPVAVICGSNEFNNDEVTIKDLARGREMSEDVEDREEWLKAEGIQVTVPRGKM
                     ISTIRKMLESK"
BASE COUNT         3656 a         3654 c         3250 g         3844 t
ORIGIN      
        1 gtagtaaagc taccatttaa tatacatcaa attgaatttg atggcaagat ataactgcag
       61 cgggaattaa tgttttggtt taattgaggt gcgtattcag cagattgtca acggattagt
      121 ggatggtcat actattccga aggctgcttg ctcggcgcgg ttctgcaaag ccggtcgatg
      181 gtttccctga tctggccctg ttcgaagggc ttggttagta tggcgtcaac tccctgttct
      241 ttcaggcgct tctgctcatc aggtctggct gagacgctga caaggcaggt acgaatttcc
      301 ggcagcctgg tcttaatcat ggcaaaatac tccaccggat ttacctcctc aagttcatca
      361 tccaggataa ccaaatccgg cttttcctca gctattatgc cgtccacaga ggcgagcgat
      421 ttagcagatt ttactttcaa cttcaaattt tgcagcagat ttgaaagcag gttcctgcgg
      481 atgcggttct ggtccacaac cacaatgttc ttgattaatc tggaatccga attcccaaaa
      541 ctgctctcag tcttcctgat tttaagcgga agcgtgatgg taacagcggt tcccttaccg
      601 ggactgcttt ctatttccag cctaccggaa tgttccttga taattctata actcaccgcc
      661 agccccagcc ccgtcccatc gggtttggtc gtgtaaaatg gattgaatac cctcggtatg
      721 gcatcttttt ctattccctt gccctggtct ttgatacaga ttacggccat gtcgccttcc
      781 gcgcgcgcgc ttattgagat ttcgccccca tgctcctcct cggcctcgac cgcattcata
      841 agaatgttga tcagcgcttc cttgatcttt ttgcggtagc cctttatcac cagctgggta
      901 tcgatattga gcctgaattc tatgacccca tgggcaatcg ccttccagcg gggcatggag
      961 agctcgatta tttcctcgat cagtccctta aactgaatca ggtccggatc gtcatcaggc
     1021 ctgaggcggg catatttttg cagttcagct atctggttcg aggcctgggt ggaggcattg
     1081 agaataatat ccaaaccctt acgggcagtt tccagcttga tgacatcgcc gctgaactgc
     1141 ctcaaaatct gggcccggcc cattatcaga gccaggtgat tgttaaaggc atgcaggacg
     1201 ccccgggtta tatcgataaa attgcgcgat ttttcgagct gcatgaaatg tttcctggca
     1261 ttgtcatagt cattcatcaa ctgctgcagg tggctgtgat ttaccgctga cttcagtgca
     1321 cctccggtaa caccgcagac cttcgatagc agcttaatat gcctgtcatc gtaagcatcg
     1381 acatctcttg tagccagatt caggcttgcc agcagttcgc ccttatcata aatcggactg
     1441 aacaaaaaag actggtagcc catccggaac attctttgtg ccaccactcc ctggcctata
     1501 tctttcaacg atgcgataaa ggccttcttt tttgataata gtccggccag gctggtctcg
     1561 ctcaatttta tgaactgctt cttgaaactg caggcatcat tctgatcgtc gtattcagcc
     1621 tcgagccgaa aagattcgaa gtccgggtga taggttacga gtgacatgtg actgaaattg
     1681 accagcttct tgagttcctt caccaaaacc ggaaaaatcg tctctatctc cacgctcgtg
     1741 agcatcatct ggctcagatg gttcagcaca ttcaggtaga ccgtgtaggc cttaattatt
     1801 ttgcccaggt atgtgcggat ataattgttc aagggaataa gattatccgc tcccagagcc
     1861 gattccggaa aaccaggcat gaatttcagt ttcaaggcca gatcaaaatc ctcatagggc
     1921 aggaaaaacg cattttcatg caggcccacc ctgcggatga gctcatgata ttccgtccgg
     1981 cttacaaatg tgcactcttg caggggcgga ctaaaatcca tgtcttcaaa tatttcggca
     2041 tagggagaat catcgggcat gatgaagtag ccgctttcca gatttttctt gaactgatta
     2101 taaattatat gactgatagt ctggttttcc gctccgcccg gcacaatcat gtcctcaatt
     2161 tcagccatgt atgtggaatt gttcgccttg gtggtcatac tgctttgtcc cgaaagatgc
     2221 tttttttcag ttcgccgaac ctgtttaatt ccttcgctat cgtctcggcg cctttgatga
     2281 taatatcgtt ataacgtatc atctccctgg catcctccgg cattttgcgg cggatcaatt
     2341 cggccgcacc cattatcccg gagagcttcg atgatatcct ggagggaagg tctgtgtagg
     2401 tctcatagac cagtgatcgt ttgtctatct cattcaagac cgcattgacc ctgttcaatt
     2461 ccagggcttt gctgtcgatc tcatgcacca gggccgatat ggtcgatacg atctgcgaat
     2521 acagcagttc ctgctgaccc agcgagcgtc gctcccagtt gcgcacttcg cctaccgaga
     2581 cgatgccgct gatacggttc tgcagccgca tgggggccag aatagcagac ttgatccccg
     2641 ggaggcccat gaactcgact tctgatttac ccatattgct ttccgggttg gactgattca
     2701 caataattgc cttgcccatc ttgatggccg ctttgtgcct gggcatcagc tcgagctcaa
     2761 cgtcctttct cgaatcagat atctgatcgg tacgtcgatg caaggaatgg tagcctatcg
     2821 agcgcaggcg gttcaaagac tgatcgtagc cccagacccg gcacatggtt gccggaagag
     2881 tttccgccag aatctgggct gaacgatcat agaaaccttt tgatcctgga ctattttcaa
     2941 gaccgaaggt gagattgaaa ctggaaacca cctgtttctt gagggtattc atcatgctgc
     3001 tgacattatt gaattctatc atacggcaca gcggcgcatt aaggaattga agatcgggtt
     3061 catccagatg attgaatgtc tttggtttta cagccgccag cgtgagcacg gccagcactc
     3121 ggccggaatc attgcgtaca ggataaacca ggcgggatga gaacccgctc ttatacagat
     3181 gataatcatc acggtaaaaa tccgactcca gcgtattggc caccagcatt cgcgcttccc
     3241 cggccgccct gagtgcggcg gtctgtttca ggggataaca tattccccgc tcaatcaaac
     3301 ttccaccgac atcggcatag gtatagcggt acatgttctg gatcgctgaa tcgagcaccg
     3361 ccaccgaaat ataatcgaat ttcaccttct cggatagaat cggatagatg cgctggagat
     3421 aagcggcaat atcgctatcc cgatcgttct ccaggtaaag ccgattctgc cggctttcta
     3481 tgtcgagccg ctcttttagc tcttcatttt gtgccgcatt ctccagcttg cttatcagca
     3541 ccgatcgcaa aatgtcctga aattccggct tcttaagtat gcgctggtat ttcggcagga
     3601 ggaatataag ttcatatgcc tcgggcaaat tgctcttcag gcacagacaa atctgtttat
     3661 gcctgcggca gagcggttcc ctatcagatg aattaagccc gtcatcgggc attcgaaatc
     3721 tgtggccgtt ctttacatca aggccatgtt gaatgctgca ggcgccgaga ttgacgcgct
     3781 gatcattttc gatttttgtg atctggtaat aatctgcact tagaattttc gccagaactg
     3841 cggaggcctc ctcgacaaac gccagtttgc ccctgttcac tgaaaccccg gcgatttccc
     3901 ccatcaggcc ggcaaaggag acggatctct tcctgaaagc gccatggttg cgaattagcg
     3961 caaagtcgat gagaaatgta attaccagaa gcagtatagc tcccgggagc agggccgtag
     4021 agaatgaatc caataaaata agaacagtcg tccccaaaat actttcgata ttcggaatat
     4081 tctgcaggag attcaaggcc gaccatccga tcagcaggac cagactgctc acgattctgc
     4141 tgtaaccgga agataaatca ggcaacagag ctgccttacg acgtttccag agccacaaaa
     4201 gcgcaagcaa caaaattgtt attatggccc agcttatttt agtcatacac aatccttcag
     4261 tataaccccc gcttataaga aatatcggat tattatggtt tcagttgagg gataaaagca
     4321 attttgatgg caattaagcc cttattttgc ttttgattag aaggaatcag ggagcttcaa
     4381 tccgctttgt attcttgaag tgcattttgg cgaccagttt cagtttatca tgaccgaatt
     4441 tttcggcaaa ttgtctgccg gtctgatcga ccagattgga tgcccctttc aggaatttaa
     4501 taccgtattg agatgagagt ctattcagat aattcaaaaa tgccccccgg gcgaggaccg
     4561 aggccgccgc cacagcaaga aatctctcgg ctcttggtgt ctgctgaagt tttatattct
     4621 ttcccttttt cataagcgaa ttttttatca gcgactcatc cccgaactga tcagaaatag
     4681 cggcccggca gtcatgcaga tccagtatgt tctcgatcac cctggcatgc ccccaggcca
     4741 gcagtttatt gagattcttc attttatcat gcagctcgtt atatttttcc gggcctatca
     4801 ccacaactga tgtatgagat aatttcttga tttcctctga aagagaaatg gcacggttgt
     4861 cggagatgcg tttggactcg aggatattca gtttttcgag atgagcctga tttccgggac
     4921 cgatatacac tcccgcaatc acaagcggcc cgaaataatc ccccttgccg gattcatcgg
     4981 tgccgatata ctcgtcggga gcctcaatga tctccaatta catgcgctcc aggtacttgc
     5041 cgtcacgggt gtcgatcttg atcttgtccc cctccttgac aaaaatcggg accttgacct
     5101 ccaggccggt ttcgactttg gcgcctttca tgacgttgga cacacgatcc cccttgatcg
     5161 ccggctcggc ctcgacaacc tcaagcacta ccgaggtggg caggttgatg tttatcgcat
     5221 ttccgcggta gaacagaatc tcatacgtat gattttccaa cagataccat ttatcatccc
     5281 cgattgcatc aaccggaatc gctatctggt cgtaggtgtc ggtatccata aaatggtagc
     5341 tctcgccgtc attatacata tactgcatat cgcgtgtctc aaacggcggt tcctcgaatt
     5401 tggtgccgga tgaaaatgtt ttctcgagca cctggccggt aacgagatgt ttcaatttgg
     5461 tggtaacatt ggcgcgcctc tgggcggtgc gggcattctg aaagtccagt attataaagg
     5521 gttcaccctc aatctcaatt ttcataccct tgaaaaaatc cgatgtatcg atcataaaaa
     5581 tagagctcct ctcgaaaaat tcaggtgtat atatattatt ttcactccct tttggcaagc
     5641 gggaaatcgg gaatatttcc tcggcagaaa tcccacccct gacggggatg gggcaccctg
     5701 gttcgcgctg gcagaaatcc cgcaccttgc ggggatgcgg tacacaggac cctgcgttaa
     5761 cagctgcaaa ttcatgaccg ccgatgagac tcaatcaaaa aaatttcgga atacatcggc
     5821 gcctggcttg tataataata aacccctgtc ggcaaaagaa ctggagggga tttttgttgc
     5881 attttcatta aacttggcta tattcgttag ctaactgtaa gtgtacaaac aataaggaga
     5941 atatggaaag aaagatacga gttctgctgg caaaacccgg actggatggt catgatcgag
     6001 gtatcaaggt tatagcttcc gccctgcgcg acgccggaat ggaggttatc tataccggtc
     6061 tgcgccagac gcctgcgatg atcgtggagg cggccattca ggaggatgtc gactgcgtgg
     6121 ggatctcaat tctttccggc gcccacatga ccctcttccc cgccatcaaa cagaaactcg
     6181 aggaaaaggg agccgaggac attatcctct tcggcggagg cataataccc ggggacgata
     6241 tcaaggcgct ggaagcgaaa ggcatcgaca agctcttcgg gccgggcaca cccaccgagg
     6301 atatcatcaa atttattgaa aatgcaattg caggcaggga caaaaaagag gaaattttat
     6361 agctcaaaat tggagggttc aactatgaaa ccgctcaatt caaaattatt gattgcatca
     6421 gttttcacat ttattgcgtt gatggtaatt ccatctacag tgatggctca ggaatacggc
     6481 aagcgtgagg ctgaggaaat ctggctgacc gagaacgatg tcgatacagc ttatgaaatc
     6541 gtgaagcttc tgagcgtcaa aaataccggc actcctaaag aatgccgccg cgagaatctc
     6601 gaggatcttt ccaagatcgc ctccaaaatg gatgcgcacg cggttgtcta tatcactcat
     6661 aatactcccg ccggtgaaaa ggacgcgctg attaccaacg gtgtcgcggt aaaatatctc
     6721 tctcccaagg aaattgaaaa gagaaacaaa aaggccaaga aaccgcccaa gctcgaaaaa
     6781 gaaagcccgc tcgatcccga accattgctg attgaataca aggatgtgga cttcccctat
     6841 aagattctgg gcattctgga aatacatacc ggaccggccg aaaccaactt ctcctcgcag
     6901 gcgatggact tccgtcttat cgatgatgct tcccgttatg gcgccgacgg cctgatctgg
     6961 gtcgattatc agcgcgtggg ttctgatgtt actgccgccc tgggtatcat gatcaaggct
     7021 tataacgact gggaaacggt tgagaaaatc aaagccaaag aagtcgagat gatgcaagcg
     7081 cgccagaagg ctgctgagga aagagccaaa gaacaggaga aagccgagga gcctgaggta
     7141 cccgacgagc cttcggagac ggcggagtag tcttaaattg gaattgaata ttttggactt
     7201 gttatacata tgaggtcaag gaggaaatga atgacttata atgtcgatcc ggaactgcag
     7261 cagccgatcc gggatatgat gcgcgagttc tgccccaagg agattgagcc tctgggactg
     7321 aaacatgacc gtgaacccga tgcgttcgct catgatatat tcaagaagct ggccgacaaa
     7381 ggactggtgg gttatgtgat gcccaaggaa tatggcggtc agggacgcaa gaaaattgaa
     7441 tatgcaacca tgatcgagga actctcatac ttcgatccgc cttcatcact tctgtcggcc
     7501 gtctcgcaac ttgcggtcga tccgattatg aatttcggca ccgatgaaca aaagaagaaa
     7561 tatattccca aagccgcaac cggtgaattg atcccggctt tcgccctgac agagcccaat
     7621 gcgggttcgg acgccgccaa tcaaagtacg gaattcgagg atgccggcga tcactatgtc
     7681 attaatggcg agaagatttt catcatgcat ggggatgtct gcgattatgc ggtaacattc
     7741 ggaaagctca aaggagacga gggtgatcgt cccaaagtct cggccattat cgttccgtcc
     7801 gatgccgagg gttttgaggg ttcgaccttg aaacataaga tgggcatgaa atacgccacc
     7861 actggaagaa tcatttataa gaatgtcaag gtacccaagg aaaacttcct ggggcctgcg
     7921 ggtaaaggct tcaagatggc tatgatgacc cttgacggcg ctcgtgtcgg tatagcggcg
     7981 cagtccaccg gtatcgccca gcgcgcccta gatgagtcga tcaagtacgc caaggaacgt
     8041 gtggcttttg gcgcaccgat cgccaaactg caggcgattc aatggatgat tgcggatatg
     8101 gctaccaaag tcgaggccgc gcgtcttttg acctacaagg ccgcccagct ccaggataag
     8161 ggcgagtcga ttaacctgtc cgcctctcag gccaagttgt ttgcctcgga agctgccaac
     8221 ttctgcgttg atagatgcat gcagattcat tccggctacg gctacatcgg agaattctcg
     8281 attgccgaga agctctaccg cgaccagaga gtatgcgaga tctacgaagg cacctccgag
     8341 gtccagcgcc tggtcattgc caacaacatg ctcagaaaat agatttctat ttactgtcaa
     8401 atgaaataag aaagcggctc cgattttgga gccgtttttt ttgaatgtca ttccgaagga
     8461 gtactgacct tcggtccagg acgactgagg aaccttttgg cgactcaatg agattcttcg
     8521 ctgcgttcag aatgacagga cgaactaaac tgtcattccg aagaagtacc gaccttcggt
     8581 ccaggacggc cgaagaatct atgacaatat atcaaaagct cagctatcat tttccatccc
     8641 ccttgagcgg cttccagtat ttgtccagca tatagtcatc gcccgagatc ggtatctcat
     8701 actcgaccgc ttttttggtc aggaagtgga ttatctcctc acggcctgtg atttcatcgc
     8761 tgattgttat actggacttc tcggacttga caaaggcctt accgttcttg agaaataact
     8821 ctgttgcatc tggaaatttt atgcataccg ctcggatgcc gatacgggcg acataattat
     8881 cggtcaggcg gattttcaac aggttgcggt cgatcactat atatccaacg aaaacgataa
     8941 tcacaatccc tgcgatcaac ctgtgataat ccaccatatc tcgaaagaag agcgatatcg
     9001 cccatacacc catcgcaatt agaactattc ccgcaaggtt atagctgatc caccaggctt
     9061 tacgtatccg gtattctttc atatcctgat ccaaaccttc aaagcaatca tattccgggt
     9121 gccccaagct tcagcttggg aaggaacgac cctggcccaa tctaaagatt tggccaccct
     9181 taacctgata tatgtgcaag gctcaatttc tttcgccaaa caaaatttct ctcatctttt
     9241 gaggaatttc cggatcgggg atatcatgat cccccgaaaa tatcaataca aaattctctg
     9301 ccaatatttc ctccggatgg agaacatact ctgtgttatg acccactttt tccataaacg
     9361 acggtacatt ggtgtaataa tacagcacag gcaggccgtc cttcatcttc ggaacataca
     9421 tgccatcatg gttttctaag gccatcaatc cgaaactcaa actctgtatc aggtttgttt
     9481 cctcggtaac ttgatgcatc ctgaccgatg acaccacaat cggaacaacc ggaatttcct
     9541 cgccctctac ctcaacttcg atataataat tattgtgagg ggcatctgga tttgtgatgc
     9601 gcctgttctt gagagtatcg ggaaattcaa tttcattgca gggataaaat tctattatct
     9661 gataaagatc gtcctgatat tccggattgt tgcgtgtcag gatgtgaaag agttcatgat
     9721 aaatcaaatg tatcagatcc gcattttgca cttcctccga tacacccgga aatataattg
     9781 cattttggcg ggtatagaca cctccgccct cttcctcacc agtcgtttta gcaaaatata
     9841 cagtgtccgg aaaatcaagt ttgatctcct gtaacaccgg aagaattgag tccatcgctg
     9901 tcttcagaat ttctatctca gcatcattcc attcaagcgc attctgtccc agaaagttca
     9961 caaatgaatc gaccgaaaca gtctcagttg tcttcatcct gatacttcga tcgattggtg
    10021 acatggcatt gatgaactca tctcttgtaa taaggatctc ctttgctgtt tccacatccg
    10081 catagacaat tttgaaaggt ttctcggcat ggaggagcat tgggaagaca aatagggcta
    10141 ttgtcaatat attaatcgca aacgtctgtt tagtcattat agttacctcc cacattgaaa
    10201 gcataagatg gtatttatcg ggcacctaag caagtaatat tttgagggtg attggaaatg
    10261 tcattttaag ggagcgtagc gaccgaagaa tctttctcga ttcatagaga ttcttcgctt
    10321 cgctcagaat gacagggtgt tagaggtttt tttgcataaa gaactttgta agggtgctcc
    10381 acgcttcagc ttgggaggga cgctgacttc gaaaatccca atctaaagat tggctcaccc
    10441 aatcaacatg ctattcctgc attttttctt caagctctac atctattcct tcgaatttcg
    10501 tgagatcatg cgtctgagcc aatgattcta attggctttt caggttttca aatctgttat
    10561 ttgtgtcttc caaatgaaaa ctatattgac gaaattcaat catgttatca gtaacctgaa
    10621 taccttttgc tttgacctgt gctaactgaa gaatatatcc atttaattgt tctaaagaag
    10681 aataaacata ttctatgctt tcatagattt ttctgaaacc aggcgaagat acaatcaatt
    10741 gataaaattc agacaatatg ctcttctcaa ttattctcaa tccaacaggc ttatattcct
    10801 tttcttttat tttttcattc aacctttcca ggtgttttct tctcagggaa agatcagata
    10861 taataacatc taaaagcctc cattttttta attctctttg ccttctctct ttttttgcac
    10921 ctaggtgata tgcataccag gcaacaataa atggggctat tacagcaatt atctggatta
    10981 tgactactat ataatcccgg aatgccattt tgtgatgtgt ttattacggg attgctaatc
    11041 gagacatcaa ctgtatctct aacctctact ggtattttca ttttacatcc cctttcaaaa
    11101 catatctatt ctacaacggt atattcccat gcttcttggg cggattttgg tcgcgtttgg
    11161 tggagagcat gtcgaaggcg cgaatcaaac gaggacgggt agtcctgggt tcgatcacgt
    11221 catcgagata accgcgctcg gcggcgatat acggattggc aaatttctca cggaactcct
    11281 cgaccttctt gtcggtggcg gcctctgggt tatcggatgc ggcaatctcc tttttgaaaa
    11341 taatctcgac cgcccccttg ggtcccatga cagcaatctc ggcagtcggc caggcataat
    11401 tcatgtctcc gcgcacatgc ttggagttca taacatcata cgccccgccg tatgccttgc
    11461 gggtgatcac ggtgatcttg gggaccgtcg cctcacagta ggcatagaga agcttggcgc
    11521 catgacggat aatcccgttg aattcctgat cggtacccgg caaaaaaccg ggcacatcct
    11581 caaaagtcag gatgggtata ttgaaggcat cacagaaacg gatgaaacgt cccgccttat
    11641 ctgacgaatt tatatccagc acacctgcga gataagcagg ctgattggca ataattccga
    11701 tcgaccgtcc gcccaggcgg gcgaaaccga cgacaatgtt catggcatat tccggcataa
    11761 tctcaaaaaa gtcaccgttg tcaacaatgc gcgtgattac gtctttgata tcatacggct
    11821 tatttggatt gtcaggcacg atatagttca attcctcatc catacggtca tcaggatcat
    11881 tgctttcgat catcggcact tcatcaagat tattctgcgg gataaaggac atcaatttgc
    11941 gggtcatcag aagcgtctca gcctcattgt taccgactaa atgagccacc ccggatttag
    12001 acgcatgtac catcgcaccg cccagctcat catgagtgat ttcttcatgc gtcacggtct
    12061 taacgacatt tggacctgtg acaaacatat atgaggttcc cttgaccatc agaacaaagt
    12121 ctgtgatagc cggagaataa accgcgccgc ccgcgcacgg ccccagcaca accgaaattt
    12181 gaggcaccac tcctgaggcc agagtattgc gcaggaaaat atccgcataa ccgccaagag
    12241 aaatcacacc ttcctgtata cgagctcccc cggagtcatt caggccgatg acaggtgcgc
    12301 cgaccttcat ggccatatcc ataatcttgc agattttttc cgcgtgggct ttcgataatg
    12361 atcctccgaa gattgtaaag tcctgcgaga agacaaagac cttgcggtcg tcgatagtgc
    12421 cgcatccggt cacaacgcca tcggtaaaat atttttgctt atcgaggcca aagtcggatg
    12481 atcgatgccg cacaaacata tcgaattcct cgaatgaccc tttatccagc agaagatcca
    12541 ggcgttcccg tgcggtcagc ttacccttct ggtgctgggc ctctattcgc ttttctcctc
    12601 cgccaagcct ggcgtcatcc tgcaattttt tcaactgttc caatttttta tctatggaca
    12661 taatacaccc gtcctgatta agactatttt tcaaacttca actttttcag ccaccagctt
    12721 catcccggta tcgattattc tgcagacttt tatatcgatt tcgtgatcga agcccagata
    12781 ccatccatgc tgggcaatat ccggcaaaag ttcctttttg attttgattg tttccagagg
    12841 aaagaggtcg gccgaggcta cataggcggg ccggatatgc gactggaacg gcacaatatc
    12901 agcaaacaga aacagcctct ccccttccga ttcgactatg actccctgat gtcccatggt
    12961 atgcccgccc gtcaggacca gcttgattcc cggaatcagt tccacattgc cctcgattat
    13021 tttcagaagc ccagcgtcat acaggggcat aaacttggat ttatcatagg ccgcttcgga
    13081 gcgctcgtcg ggatcattgg ccgcctccca ttccagcttc tgcaagatat gctgcgccct
    13141 cgaaaaacgc ggtaccggat tgccgtcacc atcatccttg acagcgccgc cgacatggtc
    13201 gagatgcaga tgtgtatgga tcacataatc gacctcatcc ggctttatgc ccaattcggc
    13261 aagtccggac tcaagcctgg attcgggtgc ggtgccgtat attttctgct gccgctctga
    13321 aagcatctcc cccaggccgg tgtcaaccag caatatcatc tcgcctgtac gaatcacgaa
    13381 aacattaaga tccatcggga caaggttctg ttcatttact tgaacatcct tcgaccacaa
    13441 ttttttgggc acaaccccga acatcatgcc gccgtcaaca taagcagtat tctcgactat
    13501 gacatcaatc tgaaatttcc cgagtttcat ggcaggcact actttgattc cagcattttt
    13561 ctaattgtcg aaatcatctt ccccctcggc acggtaacct gaatgccctc ggccttcagc
    13621 cactcctcgc gatcctcgac atcctcagac atctcccggc cgcgggccag gtcttttatc
    13681 gttacctcat cgttattaaa ttcgtttgag ccgcatatca ccgccaccgg aatatccaga
    13741 cggtcggcgt attggagctg cttggggatc cggcgattat ttccaaggaa aacctcaaca
    13801 ttccagccct gctcacgaag ctcggaggct atcttgaaat agtcgctcat acgttccctg
    13861 tccatagtgg cgacaagcac atcagtcgag gtcttctgag cttcaatcaa atcgagcttc
    13921 ttcaaagctg ccagaagccg atccaccccg atcgaggcgc cggttccggg agcttcctgc
    13981 cccgaaaacc ttcccaccag attatcgtaa cgcccccccg cgaacactga cccgaattcc
    14041 ggcaggtctg tcagtatagt ctcgaaaatc ggcccggtat agtatcccag accgcggaca
    14101 actgttgtgt caaagacgca atattccatt ccgactgacg aggccatcag atattcatgt
    14161 atctccttca gttcatccag gccgcgtttc gcgatctcgt attcacccag aatctgcata
    14221 catttttcga atgtcccctc aaaatcctct ctgggtatgt cgagaaaatg ttctatacga
    14281 gaaatctgct ccggcttcag attcagtccc ggaatttcat cgccggattt gtccacccgc
    14341 cccggtccaa gctccgcggt aaccgcttcc cgcccctgct tatccagctt atccaggaca
    14401 cgaa
//
LOCUS       DSEB01000046            6516 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000733, whole genome shotgun sequence.
ACCESSION   DSEB01000046 DSEB01000000
VERSION     DSEB01000046.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6516)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6516
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000733"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..282)
                     /locus_tag="ENO07_00930"
     CDS             complement(<1..282)
                     /locus_tag="ENO07_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS00557.1"
                     /translation="MPPKEEKLSLVGWLSFDFANTIFSMNIVTMFFNLWILDDLNCPD
                     ILYSISYSASMVMVALLMPFYGHYSDLQQNKKSWLIRFTLLSIISTVLIG"
     gene            complement(295..840)
                     /locus_tag="ENO07_00935"
     CDS             complement(295..840)
                     /locus_tag="ENO07_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDS00558.1"
                     /translation="MSREDAYKLVQEKVRQKNLVKHILAVEAVMRTLAEHFGEDVEYW
                     GLTGLLHDLDYSKTVDHPEKHTFLTEEWLEGYEIPDDMMHAIRAHPGHIPCESKLDWA
                     LYCSDPVTGLIVAGVLMHPTKKLADVDEGYMLRRFKEKRFAAGADREQIAKCQELGLS
                     LEEFMGLALKGMRSISDELGF"
     gene            complement(852..2768)
                     /locus_tag="ENO07_00940"
     CDS             complement(852..2768)
                     /locus_tag="ENO07_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00559.1"
                     /translation="MIARAISKPVELFLFILVFILSTSVAAAQEAGPEPGETDTVKAA
                     IPDTIPEKSIEYVQYTARDSLVLNSHLVRTINNTAYSTKHSNSTGEAVRTYSGFKLLR
                     TGPYAQPEYIMFGGMPVRSFVFGDFVFDYAQFGLPVNGLVDSRLIGYEQAAYAKFPFF
                     STVEHTNALHVFRPAYPDSGALTSAYMQKGDYGFSNTMVKFRGNLGERGRLGFTAGFK
                     LSDGYVTNQAKDFETYRLFADYQLSENLQLQPDLAFFSSDDELRFLNEFENYQGRSEA
                     SFVGLSMALAAKERVWILDRISFIFQRFSESVRGDHDKFRQVSQFYKLKLETSINISD
                     WYTYLNIEPFARNIEFEPETTEYAGLTASGLSRYDLLNNFFINVSGAVTISDYAENDI
                     SLAGGLSYSKSGSLILSGQLFRITRPPSDFELFASSPQIDLLPITGGEGQFSFQGNSD
                     LDNTDYIGYSLGMKFDKGIFEGDIYLKQAEISNSTWWTQNVHGSSPYQRDANWIVGGF
                     DLSLALPLGFDSRFAYSYGLLEDSDSEVYLTLAPRHNACAFLTKEIYIPGLNLYVLSG
                     LEGEYHSENFKSPYNPQTLGEYFLMNAQFQFKIKTLTIFYNMDNVFNKPHRDFYNYEL
                     RRRIWWGFLWNFRN"
     gene            complement(2765..3292)
                     /locus_tag="ENO07_00945"
     CDS             complement(2765..3292)
                     /locus_tag="ENO07_00945"
                     /inference="COORDINATES: protein motif:HMM:PF07155.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDS00560.1"
                     /translation="MTQGYTKSLVFTALFAGLAYVSNLFMLIVPNVSPAFFIIFMAGY
                     ILGPRWGFISGGLGFLLISYFSPYGMAMLPLLAVQIISGGLVGMIGALARALFPVKTS
                     DWRTYLLFGFWGFAVTSIYMGAISLADALLFGPFKERFLISLGFSMLTIVSNIIIFAF
                     LTPLNKTLREFVKRV"
     gene            complement(3289..5238)
                     /locus_tag="ENO07_00950"
     CDS             complement(3289..5238)
                     /locus_tag="ENO07_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HDS00561.1"
                     /translation="MKKTSILIAILLIVFASTSISEQLSTEQDAELYRQIMIWRGETD
                     VSLSSDSLEGKIGKCGTPLLMSISQQIKNASLKTQQAWIALLYERYDQEAPETYGSPK
                     GHFLIHYATDGVHAARTDENLGINPTTGVPEYVESTAAIFDYVYEYQIDTLGYKDPPR
                     DDFYPEGGDERFDVYLVDLATVGWGNAYGVTFPDMQFGTSGQYSTAFQALDNDYYELP
                     LYEDRPLEAVRVTAGHEFFHVTQFGYDVLEFENMGTDTDLDDRHHWVEMSAVWMEEEI
                     YDHVNDYYNYLPSYLSSIYRALYTNQPGLYQYGAVLWPKYLSEKWGRDIIKDIWERCE
                     AQIGPNVFQNAFSDAIEQFSGGEYTFGQALSELYIWNYFTGLRARDNFGFEEAGEFVN
                     YLGNPIQVPDSVREGNSYRFNILNFKDYPLEFNSVVGEFFYFPQVMGGFYLRFTGVDQ
                     LDSSLTINFKGLPSKRVLGVTYDIIWNNRLMAYNPHIPTQPIYIDPVVYAADSSEITI
                     EHDLLQQYDEFVLVLSPFVIPRANINYIDDLVFSLNVTDTSEPIETIVISEPYPNPYV
                     ISQSAGQDIYVEVMQPEPQPIRMQVYTLSGERVFESEVAASRTTEIVGWNGHNEEGDV
                     VASGMYLLYISVGDVDRVLKVAIIE"
     gene            complement(5252..6076)
                     /locus_tag="ENO07_00955"
     CDS             complement(5252..6076)
                     /locus_tag="ENO07_00955"
                     /inference="COORDINATES: protein motif:HMM:PF02811.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDS00562.1"
                     /translation="MKNPVDLHVHTNCSDGLLWPEAVIDFCLKNGIKYLSITDHDTVQ
                     AHTILSNYVANKDITLVPGVELSTSWKSTDFHILGYFIDLDNRDFKKKIDFFRQQRFK
                     RGEAIVKKLNELGIDLSMETVKKVAGQSVMGRPHVADALVKEEYVLTLDEAFARYLGY
                     HAPAYVPKKYLTPQEAIDLIHNSRGVAIWAHPGPVGKDYFIEDFIAMGLDGIEAFHPL
                     HDMRASRHYIDLAKRYDIIYTGGSDCHARRERILIGTQKVPHKCYKNLLKVKEKYQ"
     gene            complement(6073..>6516)
                     /locus_tag="ENO07_00960"
     CDS             complement(6073..>6516)
                     /locus_tag="ENO07_00960"
                     /inference="COORDINATES: protein motif:HMM:PF04961.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HDS00563.1"
                     /translation="VMMRADGLKEALFNLISDDAKAFDDVMAAMKMPKDTDKMKAERE
                     QAIQKATIQATRIPLKVMELSYEAIKCSQIVAEKGNVNSLSDAGVSSLAGRTAVIGAY
                     MNVKINIPGIEDDKVKTEIQEKAEKIRKDAVELAQKIENDVISKL"
BASE COUNT         1742 a         1591 c         1474 g         1709 t
ORIGIN      
        1 ccctattaat accgtggaga tgatcgataa taatgtaaat ctgatcagcc agcttttctt
       61 gttctgttgc aggtctgaat aatggccgta aaaaggcatc agaagagcga ccatgaccat
      121 ggatgcggag tagctgatag agtagagtat atccgggcag ttgagatcgt ccagaatcca
      181 gaggttgaaa aacatggtca ctatattcat ggagaatata gtattggcaa aatcaaacga
      241 cagccatccg accagcgaaa gcttttcctc tttggggggc atgggacaaa ccttctaaaa
      301 tcctaattca tcggatatcg aacgcatccc ctttagagca agccccataa actcctccag
      361 gctcagcccc agctcttggc attttgctat ctgctcccgg tccgcccctg ccgcaaaccg
      421 tttttctttg aagcgcctga gcatatatcc ttcatcgaca tcagcaagtt tttttgtggg
      481 atgcatgagc actccggcca ctatcaggcc ggttaccggg tcggagcagt acagtgccca
      541 gtccagtttg ctttcacagg gaatgtgtcc gggatgggcg cggatcgcgt gcatcatatc
      601 atcaggaatt tcatatcctt cgagccattc ctctgtcagg aaggtatgct tttcgggatg
      661 gtcgactgtt ttggagtaat cgaggtcatg cagcagcccg gtcagccccc agtactcgac
      721 atcctcacca aaatgctcag caagtgtgcg cataaccgct tccacggcca ggatatgttt
      781 caccaggttt ttctgcctga ctttttcctg aaccagttta tatgcatctt cacggctaat
      841 catccaacct cctaattgcg gaaattccag agaaagcccc accatatcct tcgacgcagt
      901 tcataattgt aaaaatcccg atggggttta ttaaagacat tatccatatt gtaaaagatt
      961 gtcaatgtct taattttaaa ttggaactgt gcattcatca ggaaatattc tccgagcgtc
     1021 tgaggattgt aaggcgactt gaaattttct gaatgatatt ccccctcaag accgctgaga
     1081 acatagaggt tcaatccggg aatatagatt tcttttgtaa gaaaggcgca ggcattatgt
     1141 ctgggcgcaa gtgtgagata aacttccgaa tcgctgtcct cgaggagtcc ataactgtag
     1201 gcaaatctgg aatcaaaacc caggggcaga gcaagactga gatcaaaacc gcccacaatc
     1261 cagttggcat cgcgctgata aggacttgat ccgtggacat tctgcgtcca ccaggttgaa
     1321 ttcgatatct ccgcctgctt cagatatatg tcaccttcaa aaatcccttt atcgaacttc
     1381 atgccgagcg aatatcctat gtagtcggtg ttgtcgaggt cgctgttccc ctgaaagctg
     1441 aattgtcctt cgcctccggt aatcggaagc agatcgatct gcggggatga tgcgaaaagt
     1501 tcaaaatcag agggcgggcg ggtgattctg aataattgtc ccgatagaat cagggatccg
     1561 cttttgctgt agctcagccc gccggcgagg gagatatcgt tttcggcata atcgcttatt
     1621 gttactgctc ccgagacatt gataaagaaa ttattcaaga gatcataacg gctcagtccg
     1681 gatgcggtca aacccgcata ttcagttgtc tcaggttcga attcgatatt cctggcaaaa
     1741 ggttcgatat tgagatatgt gtaccagtcg gatatgttga tagaagtttc taatttcaat
     1801 ttataaaact gggagacctg ccgaaattta tcgtgatctc ccctgaccga ttctgagaac
     1861 ctctgaaaaa taaatgaaat tctgtccaga atccataccc gctccttcgc agccagagcc
     1921 atagataagc cgacgaagct ggcctcagat ctgccctgat aattttcgaa ttcattcaag
     1981 aacctcagtt catcatcgct gctgaaaaaa gccaggtcgg gttgcagctg caggttctcg
     2041 ctcagctgat aatcggcgaa caagcggtat gtttcgaaat ctttcgcctg attagtaaca
     2101 tacccgtcac tcagcttgaa tccggctgtg aatcccaacc ggcctctttc tccgagattg
     2161 ccccgaaatt taaccatagt attggagaaa ccataatcgc ccttttgcat gtaggcgctg
     2221 gtcaatgctc ccgaatcagg ataagccggc ctgaagacat gcagggcatt ggtgtgttct
     2281 acagttgaaa agaacgggaa ttttgcatat gcggcctgct cgtagccgat cagcctgctg
     2341 tcgaccaggc catttaccgg aaggccgaac tgagcgtaat cgaagacaaa atcaccaaaa
     2401 acaaagctcc tgacaggcat gcccccaaac attatatatt ccggctgagc atacggtcct
     2461 gttctcagca gtttgaaacc gctgtatgtt ctcacggcct ctcccgttga atttgaatgt
     2521 ttggtgctgt aagctgtgtt attaattgtc cggaccagat gggaattcag aaccagtgag
     2581 tctctggctg tatattgcac atactctatc gatttttcag ggatggtatc agggatcgcc
     2641 gccttcactg tatcagtctc cccgggttca ggcccggcct cctgcgcagc cgcgacagaa
     2701 gtggacaaga tgaagacgag aataaataaa aacagctcta ccggttttga tattgctctg
     2761 gcaatcatac cctctttaca aactcccgca aagttttatt taacggagtc aggaaagcaa
     2821 agataattat attggaaacg attgtcagca ttgaaaaacc taaactgatc aaaaatctct
     2881 ctttaaaagg tccaaacaga agagcatcag cgagggaaat ggcgcccata tagatcgagg
     2941 ttacggcaaa accccagaaa ccaaagagca gatatgtccg ccagtcagag gtttttacgg
     3001 gaaagagggc acgagcaagg gctccgatca tccctaccag gccgccggag attatttgca
     3061 ccgccaaaag cggaagcatg gccatgccgt agggactgaa gtatgagatc aggaggaatc
     3121 ccaggccgcc ggaaataaat ccccacctcg gcccgagaat atagccggcc ataaatataa
     3181 taaaaaatgc cggggagaca ttgggcacga tcagcatgaa taagttcgat acgtatgcca
     3241 gcccggcaaa taaagctgta aatacaaggg atttggtata accctgtgtc attctattat
     3301 ggcgaccttc aaaaccctgt caacatctcc aacagaaata taaagaagat acatacccga
     3361 ggcgaccacg tcaccctcct cattatggcc gttccagccg acaatctcgg tggttcggct
     3421 ggcggccact tcactctcga acacccgttc accagaaagg gtataaacct gcatcctgat
     3481 aggctgcggt tcaggctgca taacctcgac atagatatcc tgccccgcag attgcgagat
     3541 tacataagga ttcgggtaag gctctgaaat gacgattgtc tcgatcggct cggatgtgtc
     3601 tgtcacatta aggctgaata caaggtcatc gatataatta atattagccc ttggaatcac
     3661 gaagggagaa agcacgagca caaactcgtc atattgctgt aataaatcat gctcaatcgt
     3721 tatttcggag gagtcagcgg catatacgac cggatcaatg tagatcggct gagtgggaat
     3781 atgcggatta tacgccatca agcggttgtt ccagatgata tcataggtaa cgcccagtac
     3841 ccttttgctc ggcaggccct tgaaattaat agtcaggctt gaatcgagct gatccacacc
     3901 ggtaaaacgc aagtagaatc cgcccataac ctgggggaaa taaaagaatt caccaacaac
     3961 tgaattgaat tcgaggggat aatctttgaa attcagtata ttaaaacggt acgagttgcc
     4021 ctctctcact gaatcaggaa cctgaatcgg gttgccaagg taatttacaa actctccggc
     4081 ttcttcaaat ccaaaattat ctctcgcccg aagaccggta aagtaattcc atatatatag
     4141 ctcagaaagc gcctggccga atgtatattc acctccggag aattgctcta tggcgtcgct
     4201 gaaagcattc tggaagacat taggacctat ttgcgcttcg catctctccc agatatcctt
     4261 gattatatcc ctgccccact tctcggataa atacttgggc cagagaactg cgccatactg
     4321 atatagtccc ggctgattcg tataaagcgc cctgtatata gaagacaaat aggatggaag
     4381 ataattgtaa taatcattta catggtcata aatctcctcc tccatccaga cagcggacat
     4441 ctcaacccag tgatggcggt cgtcaagatc cgtgtccgtg cccatatttt caaattccag
     4501 aacgtcatat ccgaattgcg ttacatggaa gaactcatgt ccggccgtaa ccctgaccgc
     4561 ttcaagagga cgatcctcat agagtggcag ttcataatag tcgttatcca gagcctggaa
     4621 ggcggtagaa tactgcccgc tggttccaaa ctgcatgtca ggaaaggtca cgccataagc
     4681 attaccccat ccgacagtag ccaggtccac aagatagacg tcaaagcgct catcccctcc
     4741 ttcaggatag aaatcatccc ggggcggatc cttgtaacca agtgtgtcaa tctgatattc
     4801 gtagacataa tcaaaaatgg cggcggtaga ttccacatat tcaggcacgc ctgtggtggg
     4861 atttatgccc agattttcgt cggtcctggc tgcatgaacg ccgtcggtag cataatgaat
     4921 aagaaaatga cctttgggcg aaccgtatgt ctccggagcc tcctgatcat atctctcata
     4981 caaaagcgca atccaggcct gctgagtttt cagcgaggca ttctttattt gctgagaaat
     5041 agacatcaaa agtggagttc cgcattttcc tattttccct tccaagctat cgctgctcag
     5101 gctgacgtcc gtctctccac gccagatcat tatctggcga tagagctcag cgtcctgttc
     5161 agtcgaaagc tgctctgaaa tcgatgtcga tgcgaacact atgagtaaaa tcgcaattaa
     5221 tattgaagtt tttttcacag ccgctcctaa atcattgata tttttctttt acctttagca
     5281 agttcttgta gcatttgtgc ggcactttct gggtgcctat aagtatcctt tctctgcgag
     5341 catggcagtc ggaaccgccg gtatatatta tatcgtatct tttggccagg tcaatataat
     5401 gcctgctggc tctcatatca tgcagggggt gaaaggcttc aataccatcc agtcccatgg
     5461 caataaaatc ctcaataaaa tagtcctttc cgaccggtcc cggatgagcc cagattgcaa
     5521 ctccacggga attgtgtatc aggtcgatag cttcctgggg agtgaggtat ttcttgggaa
     5581 catatgcggg ggcatgatag ccgagatatc gcgcaaatgc ttcatccagg gtcagaacat
     5641 actcctcttt caccagggcg tcggcaacat ggggccggcc cataaccgat tgacctgcta
     5701 ccttttttac agtctccatg gacaaatcga tcccgagctc gtttaacttt tttacgatcg
     5761 cttctcccct tttaaagcgc tgctgacgga aaaaatctat ttttttcttg aaatccctgt
     5821 tatcgagatc tatgaaatag cccaggatat gaaagtccgt tgatttccat gaggttgaaa
     5881 gctccacccc gggcaccagg gttatgtcct tgttggccac atagttagag agtatggtgt
     5941 gtgcctgaac tgtgtcatgg tctgttattg acagatattt tatgccgttc ttaaggcaga
     6001 aatcgatgac cgcttcgggc cacaaaaggc cgtcagagca attagtatgc acatgcagat
     6061 ctactggatt tttcatagct ttgatataac atcgttttcg atcttctgtg ccaattctac
     6121 agcatcttta cggatttttt cggccttttc ctgaatctcg gttttaacct tgtcgtcctc
     6181 gatgcccgga atatttatct tcacattcat gtatgcgccg atcaccgccg tcctgccggc
     6241 cagggacgac accccggcat cggaaagcga gttgacatta cccttctcgg cgacaatttg
     6301 tgagcatttt atggcctcat atgacagttc catgactttg agaggtatgc gggtggcctg
     6361 aattgtcgcc ttctggatcg cctgttcgcg ttctgccttc attttatcgg tatccttggg
     6421 cattttcatg gccgccataa catcatcgaa agccttggca tcgtcggaga taagattaaa
     6481 gagcgcttcc tttaagccgt cggctctcat cataac
//
LOCUS       DSEB01000047            2484 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1006268, whole genome shotgun sequence.
ACCESSION   DSEB01000047 DSEB01000000
VERSION     DSEB01000047.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1006268"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            1..1775
                     /locus_tag="ENO07_00965"
                     /pseudo
     CDS             1..1775
                     /locus_tag="ENO07_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778399.1"
                     /note="frameshifted; incomplete; missing start; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
     gene            1772..2296
                     /locus_tag="ENO07_00970"
     CDS             1772..2296
                     /locus_tag="ENO07_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00564.1"
                     /translation="MIAKDHPHYERIFDRSKCENVVTKRSDWTNTDEMSEELPLDLGE
                     PYCYWGSKDSRAMVDPAWIKGPHKVVKRSRIKAVALLAYEPNAPAVQKLSQEDALEYI
                     TEGKYRLPSGSGMTPFKQQPFFNPYLLGDPVDLGDLQRRNFHQLFRVADAYKINIAAI
                     PSAALKSRIKELIG"
     gene            2309..>2484
                     /locus_tag="ENO07_00975"
     CDS             2309..>2484
                     /locus_tag="ENO07_00975"
                     /inference="COORDINATES: protein motif:HMM:PF00334.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDS00565.1"
                     /translation="MANTLGIIKPDGVKRKLVGNVISRIERDGLILRGVKVLKLSKDQ
                     AQKFYAVHEGKPFY"
BASE COUNT          670 a          570 c          648 g          596 t
ORIGIN      
        1 gatcaatgcc aaggccaagg ccatgaaggc ggaggggatt aatgttatag atttctcggt
       61 gggcgagcct gatttcccga ctccgtctaa tataaaagac gctggcattc aggcgatcga
      121 ggataatttt accaagtaca ccgccaacga cggcatcccc gaactgaaag aagcgatccg
      181 tgcccgtctc aaacaggacc atgatcttga ctatgctcgt aatcagatca tagtgtcttg
      241 cggcgccaag cattgtatct ataatgtatt gatggctgtg gtcaataagg acgaggaagt
      301 cataattcct gctccctatt gggtctcata cccccagatg gttttgatgg ctgagggcaa
      361 accggttata gtgcgcacca aagaggaaaa cggtttccgg attactccgc aggaattgaa
      421 ggacaatctc aatttcaata caaaagcaat aataataaac aatcccagca atcccaccgg
      481 ctcggcttac acacgagatc agctggagga agtgtgtgaa attgccgcaa gcgaaggatt
      541 attgattgtt gccgacgaga tttacgaaaa agtgatctat gataatttca aattcacatc
      601 gattgcttca ttgagtgaaa aaatcaggga gaaaacgctg atagtaaatg gtgtctccaa
      661 gtcgtattcc atgaccggct ggcgtaggat atgcagccgg gccgcgcgac attatatcgg
      721 ccatgaatat tgtacagtca catatgactt cgaatgtcaa ctctatcgcg caaaaggcgg
      781 cagtggaggc tttctcaggg aatcaggatg ctatcagcca gatggtggcg cagttcaatt
      841 cacgacgcaa ctatatgttg aacaagctta aacgtatccc gaatatttcc tgctatgagc
      901 cccagggagc gttctaccta tttccgaata cctctgctta ttacaacacc gaatacgcag
      961 gcatgaaaat ccgtaactcc tttggactct cttattatct tctcaaagaa gccgcagtgg
     1021 ctgtaatacc tggcagcgcc ttcggggccg atgaaaatat ccgcctgtcc tatgctacct
     1081 ctatggataa tattgaagag gggaccgaca ggattatcga ggcgatgtcg aagctgaagg
     1141 agtcgcccaa atataaagaa gttgctctcc agaatgttat gaccgagccg aagaaggtga
     1201 ccgatgccaa tctggaaatc tccgttgaag agcgggatgc ccttgttcag gaagccgagg
     1261 ctgcgctgcc atacgaccgc tatttcgaat ggaacgccaa tataaacggc attatcatcc
     1321 agcttcggac caatgtcccg cacctgtatg atttctgggt cgaaaactgg tatccggcac
     1381 agcttgaatc cgatctggaa ccgcatggaa ttatctatgc tgtggatggc gtacccggaa
     1441 gaactcccta tgcctattac aaccgcgaga tgaagacggc tgtcatgttc aatacagcct
     1501 attacggcca ggttagaagc tgggcattgg gtatggtcgc agaccttagt gagcgtcttc
     1561 tggatgttca cggtgtcagg gcggcctgtc tggattatga tggcaaggcg attgctttga
     1621 taggtcccaa gggtctcaag cgcggttcga cttttataag gatgctagag gatgacgact
     1681 cgaattttgt aaccaacgat ttcgttttcg ttcgttatcg tgcatctgat gctgtggctg
     1741 atgctccgga acgtaaattt atttcaagac agtgatagcg aaagatcatc cgcattacga
     1801 gagaattttc gaccgcagta aatgtgagaa tgtcgtcacc aaacgttccg actggaccaa
     1861 tacggatgag atgagcgagg agctccctct cgacctggga gaaccatact gctactgggg
     1921 cagcaaggat tcacgtgcaa tggtagaccc ggcctggatc aagggtccgc ataaggtcgt
     1981 gaagcgttca cggattaaag cagttgcgct gcttgcatac gagcctaacg ctccggcggt
     2041 gcagaaactg tcgcaggagg atgcgctcga atatataacc gaaggcaagt atcgtctgcc
     2101 cagcggttcg ggcatgaccc ccttcaagca gcagcccttc ttcaatccct atctgctggg
     2161 cgatccggtc gatctggggg atttgcagcg ccgcaatttc catcagttat tccgtgtggc
     2221 ggatgcgtat aaaatcaata tagcagcgat cccatcagcg gcattgaagt cacggatcaa
     2281 ggagttgatc gggtaggagg aatatgcgat ggcgaacaca cttggaatta taaagcccga
     2341 cggcgtgaag agaaagctgg ttggcaatgt tatctccagg attgagagag atggtttgat
     2401 ccttcgaggt gtgaaagtgc tcaagctgtc aaaggatcag gcccagaaat tctatgccgt
     2461 gcatgagggc aagccgttct ataa
//
LOCUS       DSEB01000048            3666 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002641, whole genome shotgun sequence.
ACCESSION   DSEB01000048 DSEB01000000
VERSION     DSEB01000048.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002641"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..137)
                     /locus_tag="ENO07_00980"
     CDS             complement(<1..137)
                     /locus_tag="ENO07_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HDS00566.1"
                     /translation="MKNILLVDDDPQVSETLVGLFDQDEFRFHQLEDGTRALEFIDNN
                     SL"
     gene            complement(294..1652)
                     /gene="prsR"
                     /locus_tag="ENO07_00985"
     CDS             complement(294..1652)
                     /gene="prsR"
                     /locus_tag="ENO07_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HDS00567.1"
                     /translation="MAEDKAKILVVDDEEGIRTQIYWALAGDYNVSQAADYDTALQLA
                     EEERPDLVCLDIALTSLADDLTGIKLLKELLLVDSYLKVIMVTGNEEKETALKSISEG
                     AYDYYQKPIDLEELKLIIKRAINLRKLELENLGLSQRLEKKQKFDEIIGSSRKMLEVF
                     KLIEAVSKSDYSVLITGESGTGKELAARAIHASSDRRDKPFIAINCGAIPENLLESEL
                     FGHEKGAFTDAVSRKKGKFEYAHEGTLFLDEIGDLSFPLQVKLLRFLQDQKIERVGGK
                     ELIDLDVRVIAATNADLKRKVQENAFREDLFYRLSVITIDMPPLKERGDDVVLLAQVF
                     LERFAEENKKSGLTFTPGAIARIREYDWPGNVRELENKIKRSVILTADKKIKPSDLGF
                     ERAEKLEIQPLQKFREETESQHILNALVQNNWNVSKTASALKTSRTTLYDLMEKYHIK
                     KR"
     gene            complement(1658..>3666)
                     /gene="prsK"
                     /locus_tag="ENO07_00990"
     CDS             complement(1658..>3666)
                     /gene="prsK"
                     /locus_tag="ENO07_00990"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HDS00568.1"
                     /translation="SRVVFCIAGMLFAFLFYRVYLFYPGPDFERLPFPADLNIVMVLL
                     PFIWLLFSMVFAREDYRASLKRGKVLLLFAGSLAVVFAVAGSFHPILMLVGDYPRAHE
                     ILVTRLGQWYLLYLIIVVAMTMINIENTYRSSWGIYRRKLRPPILVLGLMLILLLFSA
                     SLVLLSGAISRLTFSIMAIVSALAAVMIAFYFLRYEPQQSGVYVRRHAVYSSIAIIII
                     GFYLILAGAIGKIIQVIGGDVKLFVSVLGAMVAFVILLALVLSRSIKERIRGTVDKTL
                     HTGQLDFEEELASFSEDIAIMLNPEELTSRILELLKNRLGIGKLYLFYTDPNQEQLRM
                     RYPREHEMAEQIRIQSDGKVAAWLFRHGEAMVMDDLVERLTKRGDKVDELEQLQRLDI
                     DITLPLIAKQRLVGILFLGPKADKKPFTHREIQFISSIGHQFSLALFSARLSEELLHA
                     RQIESFHKFTTFIMHDLKNSISMLSMLLQNYESNMGNPEFQKSAMTTVNGAVNRMQRI
                     MEKLKSGEKAETFAISDCNLNEIILNLESKLGLNDISNVEFKKDLNDVGLIRADADKL
                     SEVIRNLVINALEAMPEGGKLEIATFKADEKVMLQVSDNGSGMSSDFISNKLFKPFAT
                     TKKKGLGIGLFQSKDWVEKMRGKMVVKSIQGEGTTFNVEFPAQE"
BASE COUNT          936 a          949 c          813 g          968 t
ORIGIN      
        1 agagagttat tgtctatgaa ctccagggcc cgggtaccat cttccagctg atgaaacctg
       61 aattcatcct gatcaaaaag ccccaccagt gtttcggata cctggggatc atcatcaacg
      121 agtaatatat tcttcatctt gtaactgctc ctatacccta tattcggcaa aaattctctg
      181 agcattaaga aaatctattc cgttcagctc agctaaaatg agatttttga gggtatttcg
      241 gggcttatag gattatggtt tcgtaacgaa actgaataaa aaaatagagt tttttaacgc
      301 tttttgatat ggtatttttc catcagatca tagagcgtgg tgcgggatgt cttaagggcg
      361 gatgcggttt ttgatacatt ccagttattt tgaaccagag cattgagaat atgctggctt
      421 tcggtttcct ccctgaattt ctgcaagggc tgaatttcaa gtttttccgc ccgttcgaat
      481 cccagatcag agggcttgat ttttttgtct gccgttaaga taaccgacct cttgatcttg
      541 ttctccagct cacggacatt tcccggccag tcatactcac ggattcgggc tattgccccc
      601 ggcgtaaacg ttaagccgga cttcttattc tcctctgcaa atctctccag aaacacctgg
      661 gcaagcaaaa caacatcgtc accccgttct tttaaagggg gcatatcgat cgttataacg
      721 ctcagacgat agaataaatc ctctctgaag gcattttcct gcaccttcct cttgagatcg
      781 gcattggtgg cggcaatcac gcggacatcc agatcgatca gctccttgcc gcccacgcgc
      841 tcgattttct ggtcctgaag aaaacgcagc agcttgacct gcaagggaaa tgacaggtcg
      901 cctatttcat ccagaaaaag tgttccctca tgagcatatt caaatttgcc ttttttccgg
      961 gaaacagcgt ccgtaaatgc gcctttttca tgcccgaaaa gttcggactc cagaagattc
     1021 tccgggatag ccccgcaatt tatggctata aacggcttgt cccggcgatc ggaggaggca
     1081 tgaatggccc gcgcggccag ctccttgccg gtccctgatt caccggtgat cagaaccgaa
     1141 tagtccgatt ttgaaacagc ttcgattaac ttgaagacct ccagcatctt tctggaggat
     1201 cctatgattt cgtcgaattt ttgctttttt tccagcctct gtgaaagccc cagattctcc
     1261 agttccagct tcctgaggtt gattgcccgc tttattatga gctttaactc ctctaagtca
     1321 atcggcttct gataatagtc atatgcgcct tcacttatcg acttgagcgc ggtctctttc
     1381 tcctcattgc cggtgaccat aatgaccttc aaatatgaat ctacaagcag caattctttc
     1441 aagagtttaa tgcctgtgag gtcatccgcc agggaggtca gagctatatc cagacagacc
     1501 agatcaggtc tctcctcctc agccagctgc aaagcagtat cgtaatcagc cgcctgactg
     1561 acattataat ctccggccag ggcccagtag atctgggtgc gaataccctc ctcatcatct
     1621 actaccagaa ttttggcttt atcctctgcc atactattta ttcctgcgcg ggaaattcaa
     1681 cattgaaagt tgtgccttca ccctgaatgg acttgacaac catctttccc cgcatcttct
     1741 ctacccagtc cttagattga aacagaccga tacccagacc tttcttcttg gtcgtggcaa
     1801 agggcttgaa caatttgttg gatataaaat ccgaggacat acctgaaccg ttgtcactca
     1861 cctgcagcat cactttttca tcagccttaa acgtggcaat ttcaagtttc ccgccctcgg
     1921 gcatggcctc cagggcattt atgaccagat ttcgtatgac ctcgctcaat ttgtcggcat
     1981 cggccctgat aagcccgaca tcattgaggt cctttttaaa ttctacgtta ctgatatcat
     2041 ttaaacccag tttagattcc aaatttaata taatttcgtt cagattgcaa tctgaaatgg
     2101 cgaacgtctc cgctttctcg ccgctcttga gtttctccat aatacgctgc atccggttaa
     2161 ccgctccgtt taccgttgtc atggctgact tctggaattc cgggttaccc atattcgatt
     2221 cataattctg cagaagcatc gacagcatgg agatcgaatt tttcaggtcg tgcattatga
     2281 acgtggtgaa tttgtgaaat gattcgatct gccgcgcatg caggagctcc tcagacagcc
     2341 gggccgagaa gagcgccagg gaaaactggt ggcctataga cgagataaac tgtatctcac
     2401 ggtgggtgaa tggtttcttg tccgccttgg gccccaggaa gaggatcccc accagccttt
     2461 gcttggcaat caaaggcagg gtgatatcga tgtcaagtct ctgcagctgc tcgagctcgt
     2521 caaccttatc gccgcgtttt gtcaaccgtt ccacaagatc atccatcacc atcgcctcgc
     2581 cgtgacggaa tagccaggcg gcaaccttgc catcactttg aatgcggatc tgctcggcca
     2641 tttcatgctc gcgaggatat ctcattctca gctgctcctg attgggatcg gtatagaaca
     2701 ggtacagctt gccgataccc agcctatttt ttaacagttc caggatccgg gatgtcaatt
     2761 cttcgggatt gagcattatt gcgatatcct cagaaaagga ggccagttct tcctcaaaat
     2821 caagctgacc ggtatggaga gttttgtcga ctgtgcccct gatgcgctcc ttaatcgagc
     2881 gcgacaggac aagtgccagc aggatcacaa aggctaccat ggcccccagc acgctgacaa
     2941 acagctttac gtccccgccg ataacctgta tgatttttcc gatggcgccc gcaagtatca
     3001 ggtagaaacc gatgattatg attgcgatcg atgaatagac ggcgtgcctg cgtacataga
     3061 ctcccgactg ctgaggctca tagcgcagga aatagaaggc gatcatcacc gcagcgagtg
     3121 ccgatacgat tgccataatt gaaaatgtca gacggcttat ggctccgctt agaagtacca
     3181 ggctggccga aaaaagcagc agtatcaaca tcagccccag aaccagtatg ggcgggcgga
     3241 gttttctgcg atagatcccc cagcttgacc gataagtgtt ctcgatgttt atcatcgtca
     3301 tagccacgac tataataaga tacagcagat accattgacc gagacgggtg accagaattt
     3361 catgagcccg gggataatca cccaccagca ttaatatagg atgaaagctg ccggcaaccg
     3421 caaagaccac tgccagagac cccgcaaaca agagcagtac ttttccgcgc ttaagggatg
     3481 cccggtaatc ctcgcgggca aaaaccatgg aaaataaaag ccatataaaa ggcagcagca
     3541 ccattacgat atttaaatcc gccggaaacg gcaggcgttc aaaatccggc ccgggataaa
     3601 ataagtagac ccggtaaaag agaaatgcaa agagcattcc tgcgatacag aataccaccc
     3661 gggata
//
LOCUS       DSEB01000049            3666 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002642, whole genome shotgun sequence.
ACCESSION   DSEB01000049 DSEB01000000
VERSION     DSEB01000049.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002642"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..588)
                     /locus_tag="ENO07_00995"
     CDS             complement(<1..588)
                     /locus_tag="ENO07_00995"
                     /inference="COORDINATES: protein motif:HMM:PRK06222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HDS00569.1"
                     /translation="MELNEIAYKEELCPKITMFKIYTPELVRKAKAGQFVILRAYDHS
                     ERIPMSIGHLDREEGLLTIVVMEVGMSTAEIVRMEAGEKFMDVVGPLGLPTHIKKYGT
                     CVVIGGGVGIPPINPIARALKEEGNKVYGIIGARSRELVILEDLVKEAVDELIVTTDD
                     GSYGMKGFVTDALQKLIDDGVKIEFVMAIGPVIMMK"
     gene            complement(593..1825)
                     /locus_tag="ENO07_01000"
     CDS             complement(593..1825)
                     /locus_tag="ENO07_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDS00570.1"
                     /translation="MNILVLNCGSSSVKYQLFNMDEGIPLAKGDVTRIGMSGAVLSHK
                     PFDRPMTKVSGDILDHIMAIEHVVSILLSPNHGVIKDKSDIHAVGHRVVHGGEMFTSS
                     TLINEEVMMHLRNMIELAPLHNPHNMRGISACQKILGDIPMVAVFDTAFHHTIPDYAY
                     IYGLPYVLYKKYQIRRFGFHGTSHYYVAHRAAEILNRKFEDLNIITCHLGNGASIAAV
                     HKGKSVDTSMGFTPLEGLIMGTRSGDMDPAIILRIMGLEELSLHEARTLLNKHSGLIG
                     ISGVSSDMRDLLEAKKEGNNRARLAFDAYCYRIKKYIGSYSFAMAGLDAIVFTGGIGE
                     NSKEVRAKVMEDMDWIGAGIDAEKNNSTKAIESDISSSDAKVRTLVIPTNEELVIARE
                     TQNIVGKNVQDKEVKTSR"
     gene            complement(1825..2709)
                     /locus_tag="ENO07_01005"
     CDS             complement(1825..2709)
                     /locus_tag="ENO07_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HDS00571.1"
                     /translation="MATLNKIVIIGGGVMGQGIARVVAATGTDVILIDRDLSLAHKAY
                     DEISDKLDKEIERWGITESEKKAILARISPAGDYKPAKQAPMVIEAIQEDLEAKKLLL
                     AHLDNICQPGTILVSNTSTLSITELAASTSRPDCVIGLHFLNPVHKVPLVEIVRGLKT
                     SEKTVESIKEFASILNKTPVMVHEYPGYVTTRIIVPFLNEAMHVLMEGIASAEDIDSA
                     MKLGFGFNMGPLSLADMMGLDEVMSWMENLLHELSEHKYNPCPLLRKMVRAGQLGVKT
                     GQGFFKYDAEGNRIKEGE"
     gene            complement(3025..3534)
                     /locus_tag="ENO07_01010"
     CDS             complement(3025..3534)
                     /locus_tag="ENO07_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00572.1"
                     /translation="MKHINLILIVFIFIIAVLAVQQVRAEDKTVIYKVVISEEVMTDA
                     HAVAAWREYAQAKKKWREELFFQTFPDEKKYRYSYREELDCRKRLAEYWIDLKEGNPG
                     ISNSYLDQLAQIYNSLYFPEYVYKYFRSGSWEVRKNRFRLDEFKEWAKENLDGHDPKT
                     YSHLDKIEF"
BASE COUNT          884 a          924 c          836 g         1022 t
ORIGIN      
        1 cttcatcatg ataaccggtc cgatagccat gacaaattcg atcttgacgc cgtcatcgat
       61 cagcttctgg agagcatccg taacaaaacc cttcatgccg taggagccgt catccgtggt
      121 aacaatcagc tcatccaccg cttctttcac cagatcttca agaataacca gttctctgga
      181 gcgggcgccg attataccat agaccttgtt gccctcttct tttaaggctc gggcaattgg
      241 attgatgggg gggatgccca caccgccacc tatcacgacg caggtgccgt atttctttat
      301 atgcgtcggc agacccagcg gcccgacaac atccataaac ttttctcccg cttccatcct
      361 gacgatttca gccgtcgaca tcccgacctc catcacaacg atcgtaagga ggccttcttc
      421 gcggtcaaga tgaccgatgg acatgggaat tctctctgaa tggtcgtagg cgcgaagtat
      481 tacaaactgg cctgcttttg ccttccttac cagttcagga gtgtaaatct tgaacatggt
      541 aatcttgggg cagagttcct ctttgtaggc gatttcattt agttccattt gttcacctgc
      601 tggttttgac ctctttatct tgtacgtttt ttccaacaat attttgggtt tctctggcaa
      661 tcaccagctc ttcgttggtc gggatcacca aggtcctaac ctttgcatcg gaggaggaga
      721 tatcggattc tatcgctttg gtagaattat ttttttcagc gtcgatacct gctccgatcc
      781 agtccatatc ttccatgacc tttgcccgca cctcttttga gttttctcct ataccgccgg
      841 tgaatacgat ggcatccagg cccgccatgg caaagctgta tgaaccgata tactttttga
      901 tacgatagca gtatgcatcg aatgccagcc gggcgcggtt attgccttct ttttttgcct
      961 ccaggaggtc gcgcatatct gacgacacgc cggaaattcc tattaatccg gagtgcttat
     1021 tcagtagtgt gcgggcctca tgcagggata attcttccag tcccataatt cgcaggataa
     1081 tcgcgggatc catatcgccc gaacgggttc ccatgatcag gccttccagg ggtgtgaagc
     1141 ccatagatgt atcaaccgat ttgcccttat gtacggctgc aatcgaggcg ccgttcccca
     1201 ggtgacaggt tatgatattt aaatcctcga attttcgatt gagtatctcg gccgctcggt
     1261 gggcgacata gtagtgcgag gtgccatgaa atccaaacct gcggatttga tactttttat
     1321 agagaacata gggcaggccg tagatatagg cataatcggg gatggtatgg tgaaaggctg
     1381 tatcgaagac cgcgaccatg ggtatatcgc ccaggatttt ctgacaggca gagataccgc
     1441 gcatgttatg cggattgtgc aaaggggcaa gctcgatcat attgcgcagg tgcatcatga
     1501 cttcctcgtt tatcagtgtc gaactggtaa acatctcacc cccgtgaacc actcggtgcc
     1561 cgaccgcatg aatatcggat ttgtctttga ttacgccatg gttgggggag aggaggatcg
     1621 agacgacatg ttcgatggcc ataatatgat ccagtatgtc gccggaaacc ttggtcatcg
     1681 gcctgtcgaa tggtttatgg cttagcaccg caccgctcat gccgatacgg gtaacatccc
     1741 ctttggcgag gggaataccc tcatccatat taaaaagctg atatttgacc gatgacgatc
     1801 cgcagttcaa aaccagtatg ttcattattc gccctccttt atacggttgc cctcggcgtc
     1861 atacttgaag aagccctggc cggtcttcac tcccaactgg ccggcgcgaa ccatctttct
     1921 cagcaggggg cagggattat atttatgctc ggaaagctcg tgcagaagat tttccatcca
     1981 gctcatgacc tcgtccagac ccatcatatc cgccaggctc agcgggccca tgttaaatcc
     2041 gaatccgagt ttcatcgcgc tgtcaatatc ttccgccgag gcaattccct ccattaaaac
     2101 atgcatggcc tcattcagaa acggcacaat gatcctggtg gtaacatagc cgggatattc
     2161 atgcaccatg accggggtct tgttaaggat tgaagcaaat tctttgatgc tctcgacagt
     2221 cttttcagat gttttcaggc cgcgcacgat ttccaccagc gggaccttgt gtaccggatt
     2281 caggaaatgc aggccgatta cgcaatccgg gcgggatgtt gaagcggcca gctctgtgat
     2341 agaaagggtg gaggtattgg aaaccaggat tgtgccgggc tgacagatat tgtcgagatg
     2401 agccagcagg agcttcttgg cctccaggtc ttcctgaatt gcttcgataa ccatgggagc
     2461 ctgcttggcc ggcttgtagt cgccggccgg ggagattctt gccagaatgg ctttcttttc
     2521 tgactcggtg ataccccagc gttcaatctc tttatcgagc ttgtcggaaa tctcatcgta
     2581 ggccttgtgg gcgagactga gatctctatc gataagtata acatcggtac cggtagcagc
     2641 aacaacccgg gctatcccct ggcccatcac tccgcctccg atgattacaa ttttatttaa
     2701 tgtcgccata aaatcctctt caaaaatgtt tcatcctccc gacgatctta aatatctgga
     2761 acatcgggat tttgaatata cattatcaga cgtataattc aagataaatt ggccataatg
     2821 gagcttaatt atactaatta ttattgcctg cacaaacggg cgaaaagccc ctttcctctc
     2881 atcgaaagtt taatattttt gtacgctttg cgcgcctttc tgaatggtaa aatctgggcc
     2941 gggaagcaaa taaaaaagcc gccaacgttg gcggcctctc tgtcaaatgc tcaaaggact
     3001 tgagcttgaa aagactcaat ttgtttaaaa ttcgatttta tccaggtgcg aataagtttt
     3061 tggatcatgc ccatccagat tctctttggc ccattctttg aactcatcaa gcctgaatcg
     3121 gttcttcctt acctcccatg atccgctgcg gaagtattta tagacatact ccggaaaata
     3181 tagggaatta taaatctggg ccagttgatc cagataagag ttggagatgc ccgggtttcc
     3241 ttcctttaga tcgatccagt attccgccag ccgtttgcgg caatccagct cctctctgta
     3301 ggaataacga tatttctttt catcgggaaa tgtctggaag aaaagctctt ccctccattt
     3361 ttttttggct tgcgcatatt ctctccaggc ggcaacagca tgagcatccg tcataacctc
     3421 ctcggaaatc acgaccttat aaatgaccgt cttgtcttcc gccctaacct gctggaccgc
     3481 taaaaccgcg ataataaata taaatactat taatataaga ttgatgtgtt tcatatcccc
     3541 aattgctcca gtttgcgctt tcattaacta taatcggatg aagaagcgct gaagttcagt
     3601 ccggatcgag cacaaatttc aaacaggtct atgtaaattt catacagtcc tgaaatatat
     3661 ttgcac
//
LOCUS       DSEB01000050            2186 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1008331, whole genome shotgun sequence.
ACCESSION   DSEB01000050 DSEB01000000
VERSION     DSEB01000050.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1008331"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..714)
                     /gene="radA"
                     /locus_tag="ENO07_01015"
     CDS             complement(<1..714)
                     /gene="radA"
                     /locus_tag="ENO07_01015"
                     /inference="COORDINATES: protein motif:HMM:PRK11823.3-5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDS00573.1"
                     /translation="MPKASAKTVYVCQSCGAIHAKWSGRCASCGEWNTLTEEIQENKS
                     AKKKISGSKSNVETYRLSEITGDTSYRIVSGNSEFDRAVGGGVVPGSSILIGGDPGIG
                     KSTLLLQQSGLYAGEGFMSLYVTGEESQTQIKIRADRLGIDTDMIDVACLVDTDEIIT
                     ALNQREYKFLIIDSIQTLKSAQLESSSGTVSQIRESVSTILDICQVKEITAFLIGHVT
                     KEGMIAGPKVLEHMVDVVLY"
     gene            complement(771..1493)
                     /locus_tag="ENO07_01020"
     CDS             complement(771..1493)
                     /locus_tag="ENO07_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDS00574.1"
                     /translation="MIRLEKVYKSFGTNEVLKGIDLEINSGDSMVIFGASGSGKSVML
                     KLIMGLLKPDEGDIYIDGKNINELKRSELDELRTRFGMLFQGAALFDSMTVYENVTLG
                     RSEHGDMSKQDRIECARRNLEMVGLAGTEYKYPAELSGGMKKRVGLARALCMEPEIIL
                     YDEPTTGLDPVSADKINDLIIELRDKLNITSVAVTHDIYSAFRIGRRFAMLYEGNIRF
                     DGTKEEMESSDDQVLKDFLSHH"
     gene            complement(1497..2135)
                     /locus_tag="ENO07_01025"
     CDS             complement(1497..2135)
                     /locus_tag="ENO07_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDS00575.1"
                     /translation="MLMMGVRSLPLVILTSVFTGSVTAWQAAYQFKGLVPLEWLGLTV
                     AKTVFIELGPVLTSLVLAGRVGASIAAELGTMRVTEQIDALESMGINPVGYLVMPRLV
                     AGMIMVPVLVMFANIIAILGGMVVAVMLVNISYETFTDSIKSYVYIRDVVSGLQKSVG
                     FGAIIALMGCYNGFNAKGGAEGVGLATTRAVVLASVLILVSDYIMATILFNV"
BASE COUNT          534 a          595 c          499 g          558 t
ORIGIN      
        1 atacaaaaca acatcaacca tatgttcaag taccttgggt cctgcgatca taccctcctt
       61 ggtgacatga ccaatcagga aggccgtgat ctccttgacc tggcaaatat cgaggattgt
      121 gctgacagat tcacgaatct ggctgacagt cccgctggag gattccagtt gtgccgattt
      181 taaggtctgg atagagtcta tgatcaggaa tttgtactcg cgctgattca atgctgtgat
      241 gatctcatcc gtatcgacca aacaggcgac atcgatcata tcggtatcga ttcccaagcg
      301 gtcggctcga attttgatct gggtctgcga ttcctcaccg gtgacataga ggctcatgaa
      361 tccctctccg gcgtataatc cagactgctg cagaagcagg gtggacttgc cgattccggg
      421 atcgccgccg atcaagatcg atgatccggg cacaactccg ccgcccactg cgcggtcgaa
      481 ttcgctgttg cccgagacaa ttctgtacga tgtgtccccg gtgatttctg aaagcctgta
      541 cgtttcgaca ttggattttg agccgcttat tttcttttta gcgctcttat tttcctgaat
      601 ctcctcggtc aatgtgttcc attcaccgca cgaggcacat cgcccggacc atttagcatg
      661 gatcgcaccg catgactggc agacatagac tgtttttgca gacgctttgg gcatagtccc
      721 ttaatatatg atttgcgggg gagaagccaa gcaagaaatt tgagatatga ttaatggtgg
      781 ctgaggaagt ccttcaggac ctgatcatct gaggattcca tctcctcttt agtgccgtca
      841 aacctgatat tcccctcata taacatagcg aaacgccggc cgattcggaa agcggagtat
      901 atgtcatgcg taacggccac agaggttata ttaagtttgt ccctcaattc gattatcagg
      961 tcattaattt tatctgctga gaccgggtca aggcctgtgg tcggctcgtc ataaagaatg
     1021 atctccggtt ccatgcataa tgcccgcgcc aaacccacac gcttcttcat tccgcccgag
     1081 agctcggccg gatatttata ttctgtacct gcaagaccta ccatctcaag atttctccgg
     1141 gcgcattcta ttctatcctg tttgctcata tcgccatgtt ccgatcttcc cagggtgaca
     1201 ttttcataga ccgtcatgga atcgaaaagc gccgcgccct ggaagagcat tccgaagcgt
     1261 gtgcgcaact cgtccagttc cgagcgttta agctcgttta tgttcttacc gtcaatataa
     1321 atgtcgcctt catccggctt caaaagaccc ataatcagct tcagcattac gctcttaccg
     1381 cttccggagg ctccgaaaat aaccatgctg tcgccgctgt ttatctccag gtcgataccc
     1441 ttcaggacct cgttagtgcc gaagcttttg tatacttttt cgagtcttat catttcttat
     1501 acattgaaca gtatggttgc catgatatag tctgaaacca ggatcaaaac agaggccaga
     1561 accacagccc tggtagtggc caggcccaca ccctccgctc ctcccttggc attaaaaccg
     1621 ttataacatc ccatcagtgc gataattgcc ccgaaaccca ccgatttctg caggccggag
     1681 acgacatccc gaatatagac ataggatttg atactgtctg tgaaagtttc ataacttata
     1741 ttcacgagca tgacagccac caccatgccg cccagaattg caattatatt cgcaaacatg
     1801 actaatacag gcaccataat cattccggcc acaagccgcg gcatcaccag gtatccgacc
     1861 ggattgatgc ccatcgattc gagcgcatca atctgctcgg ttacacgcat tgttcccagt
     1921 tcggcggcga tcgaggcgcc cacacggccg gccagtacaa ggctcgtgag caccggaccc
     1981 agctcgatga agactgtttt ggcgactgta aggcccagcc actccaaggg aaccagacct
     2041 ttgaactgat aggccgcctg ccaggctgtt accgagccgg taaagacgga agttaagatt
     2101 accagcggca gagaacgcac ccccatcatg agcatctgtt cgaaaatcag cctgattctc
     2161 ttgaagattg tgggaacgga caggat
//
LOCUS       DSEB01000051            3350 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003225, whole genome shotgun sequence.
ACCESSION   DSEB01000051 DSEB01000000
VERSION     DSEB01000051.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003225"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(98..652)
                     /locus_tag="ENO07_01030"
     CDS             complement(98..652)
                     /locus_tag="ENO07_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00576.1"
                     /translation="MTLAGVKTDLPVWFIKGEVRGETYRNLMRYICRHCKYFSMVTRD
                     AITLEENARKFMSTISPHLKETREKQYEWPGTRISREYEQLLGKDWFEVQGATLRIYD
                     CSNEALEYILRVTDSLFQFVHPDYPEDFTCYRDVDDPFLVTVSHEEIGAIIAGDKERK
                     QIEQIVPGLKLVEEKGFREEPNDN"
     gene            complement(839..2260)
                     /gene="glnA"
                     /locus_tag="ENO07_01035"
     CDS             complement(839..2260)
                     /gene="glnA"
                     /locus_tag="ENO07_01035"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_431129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDS00577.1"
                     /translation="MAMVTEKIKKLVKDNAVEFVDVKFIGLLGAWHHITVPIERLTNR
                     LFKNGIGVDGSSVSGFAHIKSGDMILLPDPETAFIDPFWDRPTLSFIGNVVNVNGTIE
                     QFTRDPRWIAEKAEKYLRKSKIATESIWGPEFEFYLFDHVSYDQKPEAGYYYIDSSEA
                     EWRSSDSSEANLGYQVPYKGGYHVIPPKDKTYQIRNEMTDILKSCGVPVKYHHHEVGG
                     AGQQEIEVMFGSLKDMADRSMIVKYVVKNTAFKYRKSATFMPKPLYDEPGNGMHVHQY
                     LAKAGKSIFYDPEDEIGLSQIGKWYIGGILKHASSLLCFTSSSTNSYKRLVPGFEAPV
                     RGTYSVGNRNACIRIPGYQLDKSTYRMEFRPPDATCNPYLAYAAMLMAGLDGIKNKID
                     PGFPCDDDLSAMTAEELAKIPQLPSSLQRACESLAEDHEYLLEGDVFTDDVIAVWLEL
                     KMSQVDAIRVRPHPYEFRLFYDC"
     gene            2395..>3350
                     /locus_tag="ENO07_01040"
     CDS             2395..>3350
                     /locus_tag="ENO07_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00578.1"
                     /translation="MKFLTSIMSLLLLFNIAIAQENPEDEDPFKTALSEVGLTKETLR
                     FDYGDMANYGGDKFALPLFYTLHSSPFKVELYTRLFRDNLLQNCRSALSLTAFSSQRI
                     NAGIRRGLIASPIDRVKPLLDSQTPLHDAIVSFEAFYTIPMDQDRRDELKEEAAQVPL
                     DLQKMAAFIIFTAIDSSRYHYHAFKEVREDYDLREMREKFVTFIASETDVSDMDMEPF
                     LEEVDFKYLYAPAQDLAMALDFVSDSLSKMSFNQEFEFEWQTSAGKVLINDNRKHEYS
                     GEEAYFLIIDAGGDDVYSNAASNIEIRNWISILIDVEGNDTYT"
BASE COUNT          855 a          829 c          782 g          884 t
ORIGIN      
        1 gaatgggaat cgagacccgg cacatccttc atgagggccg gttgatcgag cacggtaaac
       61 tgaggcatga attcaaagct ggtcagatcg atttgcttta gttatcgttc ggttcttccc
      121 gaaatccttt ttcctccacc agctttaacc cgggcacaat ttgttctatt tgtttgcgtt
      181 ctttgtctcc ggctataatg gcgcctattt cttcatgcga cacggtcacc agaaaaggat
      241 cgtccacgtc acgatagcag gtgaagtcct cagggtaatc cggatgaacg aactgaaaca
      301 ggctgtctgt cacccgcagg atatattcca gggcctcatt tgagcagtca taaatcctca
      361 aggtcgctcc ctgaacctcg aaccagtcct tgcccagaag ctgctcatac tctcgggaga
      421 ttcgggtccc cggccattca tactgtttct cacgggtctc tttcaggtgc gggctgattg
      481 tgctcatgaa tttcctggca ttctcctcca gcgtaattgc gtcccgcgtg accatgctga
      541 aatatttgca gtgtctgcat atatagcgca tgagatttct gtatgtctcc cctctgactt
      601 ctcccttaat aaaccagacg ggcaaatctg tttttacacc tgccagagtc ataactacat
      661 acagcctttc tgactttggt tatccgggct atgcttgctt atcgccagga gccaaatata
      721 aaaaaactgc ttcaaaatac aagactttaa tttccatcat tgtaactgtt accgtgtatg
      781 aaaaatgccc gcgattaatc gcaggcattt tcctgattac caaatatctt aatcagatct
      841 aacagtcata aaaaagcctg aattcgtagg gatgagggcg tacccggatg gcatccacct
      901 gggacatctt cagctccagc cagaccgcga taacatcatc ggtgaagaca tccccctcaa
      961 gaagatattc atgatcctcg gccagcgact cgcaggctct ttgaagtgac gatggcaact
     1021 gtggaatttt tgccagttct tctgcagtca tggccgaaag gtcgtcatcg caggggaaac
     1081 cgggatcgat cttgtttttg atcccatcca gaccggccat aagcatggcg gcataggcaa
     1141 gataaggatt acaggtggca tcgggaggac ggaactccat ccggtaggtc gatttatcga
     1201 gctgatagcc gggaattctg atgcaggcgt tgcggttgcc gactgaatat gtccccctga
     1261 caggggcctc aaaacccggt accagacgtt tgtatgaatt ggtcgaggat gaggtgaagc
     1321 ataagagcga gctggcatgt ttcaatattc cgccgatata ccatttacca atctgcgaaa
     1381 gtccgatttc atcttcggga tcgtagaaaa tcgattttcc cgctttggcc aggtactgat
     1441 gtacatgcat gccgttgccc ggctcatcat aaagaggctt gggcatgaag gtggccgatt
     1501 tgcggtactt gaaggccgta tttttgacta cgtacttgac gatcattgac cggtcggcca
     1561 tatctttcaa actgccaaac atcacctcga tctcctgctg gcccgctccg ccaacctcgt
     1621 ggtgatggta cttaaccggc accccgcagg attttagaat atcggtcatc tcatttcgaa
     1681 tctgataagt cttatccttg ggcgggataa catgataacc ccccttgtaa ggcacctggt
     1741 aacccaggtt ggcctccgag gagtcgctgg aacgccattc cgcttcgctg gagtcgatgt
     1801 aataatagcc cgcttcaggt ttctggtcgt agctgacatg atcgaaaagg tagaattcga
     1861 actccgggcc ccagatcgat tctgtagcaa ttttgctctt ccgaagatac ttctcggcct
     1921 tctccgcaat ccatcggggg tcgcgggtaa actgctcgat agtaccgtta acattaacca
     1981 cattaccaat gaagctcaac gtgggacgat cccagaaggg gtcgataaaa gcggtttcag
     2041 gatcgggcag aaggatcatg tcacccgatt tgatatgcgc aaatcccgaa accgaggagc
     2101 cgtcaactcc gataccgttc ttgaataaac gattggtcag gcgctcaatg ggtactgtta
     2161 tgtgatgcca ggctcccagg aggccgataa acttgacatc cacaaattca accgcattgt
     2221 cttttactaa tttctttatc ttttcagtta ccatagccat ctccttgtga aaaaaatatc
     2281 atagtgaaga taaaaactat tctgcataaa gcaatcaaaa tcgggcttta atccatttct
     2341 aattgacttt ttggcaataa attccaattt tcaagaaaac ggaggagggt ttatatgaaa
     2401 ttccttacat ctataatgtc tctattactg ctttttaata tagccatagc acaggaaaat
     2461 ccggaagatg aggacccgtt caaaacggcg ctctcggaag tcggcctgac aaaggaaact
     2521 ctgagattcg attacggcga catggccaat tacggcgggg acaaattcgc ccttccgctt
     2581 ttctacacct tgcactcctc ccccttcaag gtagagcttt acacccggct ctttcgggat
     2641 aatctgctgc agaattgccg ttctgcgctg tcactgacag cattctcatc acaaaggatc
     2701 aacgcgggca tccgccgcgg cctgatcgcg agcccgattg acagggttaa gccgctatta
     2761 gacagccaga ctccgcttca tgatgctata gtgagcttcg aggctttcta tacaatcccc
     2821 atggatcagg accggcgtga tgaacttaaa gaggaggccg cacaggtgcc gctggacctt
     2881 cagaagatgg ctgcatttat tatttttaca gccattgact catcgagata ccattaccac
     2941 gctttcaaag aagtccggga agattacgat ctgagggaga tgcgggaaaa attcgtgaca
     3001 ttcatagcct ccgagacgga tgtcagcgat atggacatgg aaccgtttct ggaggaggtc
     3061 gatttcaaat atctgtatgc tcctgcgcag gatctcgcta tggcccttga ttttgtatcc
     3121 gattctcttt ctaagatgag cttcaaccag gaattcgaat ttgaatggca aacttcggcc
     3181 ggaaaagtgt tgatcaacga taacaggaaa catgaataca gcggggagga agcttatttt
     3241 ctcattatcg atgctggcgg ggatgatgtt tacagcaatg ccgcctccaa tatcgaaatc
     3301 cgaaactgga tctcgatcct gattgacgtc gaggggaatg atacttatac
//
LOCUS       DSEB01000052            3388 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003142, whole genome shotgun sequence.
ACCESSION   DSEB01000052 DSEB01000000
VERSION     DSEB01000052.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003142"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            17..1402
                     /locus_tag="ENO07_01045"
     CDS             17..1402
                     /locus_tag="ENO07_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00579.1"
                     /translation="MLYQSLIAGDVAFIVADTTNIGSDTMFVNRIEGKLGYGVRLIDD
                     DAVDTFSNWSGNFMGIIISDLAYSANVASLRDTSIGIFTMDRYTETEFGLANSDYRPG
                     GHGRRLVNNRNVKYLCSTEADTIFPYQYDNQYLYYYGDLAAGAVVPFNTPDFVSRDTA
                     CVILLDRDSVTVDGDSARARRAFCGIFRIPEIMDYCHSWRLFDRLTTWVCEDTTNDWI
                     TGYECWNGHLEIDACWCEMTTAQNDSATYNTEFRFGYDLDDIISFWRLISPERKGHGG
                     FACDSLQLLFPIFALGFNGAPPDTIMDVRYTAYKIIKDEKWHGPPPDAGGDPYELDST
                     WVTRWDCVSGSSPVPWATLDLRAGIDYDVSALDTFRLSYPEDSIGDIIRFNIPGPVFD
                     SWAGDTSNNNGLVLKVTAVYDSLSNIEYSVHSPIENALSNSMRIQAWFSPVEEPGVGL
                     KSNVIGTGLIE"
     gene            1448..2554
                     /locus_tag="ENO07_01050"
     CDS             1448..2554
                     /locus_tag="ENO07_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00580.1"
                     /translation="MKRLKVIIFCLLPVLLNTASKSYGFDIFPEVVGNGVSDSIILTF
                     ETFDTTALPLAADPDSLKILRFGPDGSLVDSLGEDASSLENIRVGSYTVHFRGSDGSG
                     TKGRYLIRVYAYVGDEIRGAASAGYYVKSGNWDDLDSAKLYAKAILDTLQDGFASQTN
                     QANLDVPVSSCGAGSGAYPCSLYVFSSADSSALQGISLRFMNSSQTATEAVGLSNSEG
                     LVVASLDEAIYRVWAYKAGIEFEGLPDSVNMLAPSTVDTIWGSGFDPGQPVSPELCRI
                     YGWVFDLSGYAIAGVTVMARLNESPVSHDGSVISPYYRTASTDSTGYWYLDLIPSDNL
                     EPVCEYEIVIYHDSGRIARKSVAVPDQASWELDW"
     gene            2955..3263
                     /locus_tag="ENO07_01055"
     CDS             2955..3263
                     /locus_tag="ENO07_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00581.1"
                     /translation="MLDFLSFENAITAFLGLVVAFIGKKYLLPLLEVESRRKYAEWIA
                     HIADELTDDLVARYPDNRWVKFIDDSVDKLMEICGIEKEVAARAINSALRRKSLEVKI
                     "
BASE COUNT          789 a          743 c          993 g          863 t
ORIGIN      
        1 aataatagcg atgcttatgc tgtatcagtc gttgattgcg ggtgatgtgg cctttatagt
       61 ggcagataca acaaatatcg gatctgatac gatgtttgta aacaggatcg agggtaagct
      121 cgggtatggt gtgcggttga tcgatgacga tgcggtggat acattttcga actggagcgg
      181 caattttatg ggaatcatta tatccgacct cgcttattcc gcaaatgtgg catcgcttcg
      241 agacacgtca atcgggatat ttaccatgga tcgatatact gaaactgaat tcgggctggc
      301 caacagcgat tatcggcccg gcggacatgg aaggcgcctg gtcaataaca gaaacgtaaa
      361 atatctttgc tctactgagg cggatacgat cttcccttac cagtatgata atcaatacct
      421 gtattattac ggtgacctgg cggcaggagc ggttgtgccg ttcaatactc ccgattttgt
      481 ttcccgtgat acggcctgcg tgattttgct ggacagggac tctgtcacgg tcgatggcga
      541 cagcgccagg gcgcgcagag ccttttgcgg gatttttcgc atccccgaaa ttatggacta
      601 ctgtcattca tggaggcttt ttgaccgcct gacgacatgg gtctgtgagg ataccaccaa
      661 tgactggata accggctatg aatgctggaa cggacacctg gagatcgatg cctgctggtg
      721 cgagatgacc acggctcaga acgattccgc gacttacaac acagaattca ggttcgggta
      781 tgatctggat gatatcatca gcttctggcg gttaatcagt cctgagcgca agggacatgg
      841 cgggtttgca tgcgacagcc tgcagctgct ctttccgatt tttgctctgg gatttaacgg
      901 agccccgcct gatacaatta tggatgtgcg ttataccgca tataagataa tcaaggatga
      961 gaaatggcat ggtccgccgc ccgatgccgg aggcgatccc tatgagctgg attcgacctg
     1021 ggtcacgcgc tgggattgtg tttcagggag ctcgcctgtt ccgtgggcta ctctggacct
     1081 cagggcggga atagactacg atgtctcagc gctggataca ttcagactca gttaccctga
     1141 agattctatc ggggatatta tccggtttaa tattcccggc ccggttttcg attcatgggc
     1201 aggggacaca tcgaacaata acggcctggt tctgaaggtg acagcggtat atgactctct
     1261 gtcgaatatt gaatacagcg tacattcgcc gatcgaaaat gccctctcga attccatgag
     1321 gatccaggcc tggttcagcc cggtggagga accgggtgtg ggtttgaaat ccaatgtgat
     1381 cgggacaggt ttgatcgagt gaccgggaag aaatagaatc aaatgagata tggggtgaac
     1441 gatgaggatg aaaagactga aagtgataat attctgcctg ttgccggtgc ttttgaatac
     1501 ggcgtcaaaa tcgtatggct tcgatatatt tcccgaggtc gtcggcaatg gcgtttctga
     1561 cagcatcatt ttaacattcg agacattcga tacgaccgct cttccgcttg ccgccgatcc
     1621 agacagcctg aagatattgc gtttcggacc ggatggcagc ctggtggatt cgctggggga
     1681 ggatgcctcc agtctggaaa atatccgcgt cggcagttat acagttcact ttcggggatc
     1741 ggatggttcg ggtaccaaag gcagatattt aatcagggta tatgcttatg tcggggatga
     1801 gattcgaggt gcggcctcag cgggatatta tgtcaagagc ggcaactggg atgatctgga
     1861 ttctgcaaag ctttatgcca aagctattct ggacactttg caggacggct ttgccagcca
     1921 gacaaatcaa gctaatctgg atgtgccggt gtcctcatgc ggggcgggaa gcggggcata
     1981 tccgtgcagt ttgtatgtgt tcagttcggc ggactcatcg gcgctgcaag gaatttcgct
     2041 cagattcatg aattccagcc agacagcgac agaggctgtg ggcctgagca attccgaggg
     2101 cctggttgtg gcctcactgg atgaggccat ctacagggtt tgggcctaca aggccgggat
     2161 tgaattcgag ggcctgccgg attctgtaaa tatgctggcg ccgtcaactg tggatactat
     2221 ctggggcagc ggctttgatc cgggccagcc ggtctcgcct gagttgtgcc gtatatatgg
     2281 atgggtcttc gatctttccg gctatgctat tgcgggtgtg acggtgatgg cgcgtttgaa
     2341 tgaatcgcct gtcagtcatg acggatcggt gatttcgccg tactacagga cagcttcgac
     2401 agacagcacc ggctactggt acctggattt gataccgagc gataatcttg agccggtttg
     2461 tgagtatgag atcgtcattt atcatgattc ggggcggatt gccaggaaga gtgtggcagt
     2521 gcctgatcag gccagctggg agctggactg gtagagttgc aattgttggc ttcctgtcat
     2581 tgcgaaaagc ccgagttcgg cttatgctgg acgaagccac atggcaatct cgaagatgcg
     2641 tctgccaatc gtcattgcga gtccggccag ggccggaggt ggcaatctca tggacggggt
     2701 tgcaagagaa agcggagatt cccacgtcgg agttgaccag ggtcaactcc tcctcggaat
     2761 gacgccgggc ggctggccgg gtcaaactgg ggggtggccg ggtaaaactt cgtttggccc
     2821 ggatcgcttg catgcccaaa accagcctta gatcaggcca ctaacggatc ttagcgcgcc
     2881 actcttttat agagaaggaa ttggcggcgc acgagacgta cgccgtccac aataaataag
     2941 gaggtataat acatatgctg gactttttga gttttgaaaa tgccattacc gcttttctgg
     3001 gattggtggt ggctttcata gggaagaagt acctgcttcc gcttctggag gtggagagcc
     3061 gccgaaagta tgctgaatgg atagcgcata tagccgatga actcaccgat gatcttgtgg
     3121 cgagataccc ggataatcgc tgggtgaagt tcatcgatga ttcggttgac aagctgatgg
     3181 agatctgcgg gatcgagaag gaggtggccg cccgggcgat caattcggcg cttcgaagga
     3241 aaagcctgga ggttaaaata taagggtaga atcccctctt tctgagggga tttttttata
     3301 gctatcgcta attatcgata catttgccta aaatattgga acattcaggt atataagatt
     3361 ataggaataa atagatcata gttttgct
//
LOCUS       DSEB01000053            2038 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1009672, whole genome shotgun sequence.
ACCESSION   DSEB01000053 DSEB01000000
VERSION     DSEB01000053.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1009672"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..223)
                     /locus_tag="ENO07_01060"
     CDS             complement(<1..223)
                     /locus_tag="ENO07_01060"
                     /inference="COORDINATES: protein motif:HMM:PF00430.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HDS00582.1"
                     /translation="MEDIGKWQMLLTHAVGFLIALWILKKFAWQPIINILEERRQKIQ
                     SDFDEAAEDRKKSEQLLSEYEEKLKEIDAE"
     gene            complement(294..533)
                     /gene="atpE"
                     /locus_tag="ENO07_01065"
     CDS             complement(294..533)
                     /gene="atpE"
                     /locus_tag="ENO07_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013182193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDS00583.1"
                     /translation="MDFSTALSFSLPIGLALAAIGSGLGLGRAVGSAMEAMGRQPEAA
                     GKIQVGMIIGAALIEALTIYSLVGFFILSGKIGAH"
     gene            complement(561..1367)
                     /gene="atpB"
                     /locus_tag="ENO07_01070"
     CDS             complement(561..1367)
                     /gene="atpB"
                     /locus_tag="ENO07_01070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit A"
                     /protein_id="HDS00584.1"
                     /translation="MIIPGITNLVTAHEGGTPELPHFIEFLHIYLTGPFVNFLFEYKD
                     VIFGFLVLIFMCAVSMRVYAKRSLHPGKLQNLLEMIVEGFQSFFISILGDRWGRHYTP
                     FLGTLFMYILLMNWFGLIPFGKSPTANWATTAPLAIIVFLYVQYTGMRRMGFLRYLDH
                     LAGEPRSAITWALVPLNLPIHIIGELAKPLSLSLRLFGNITGEDVLLAAFTGLGVMVL
                     AFIGSPVGLPLQLPFYFLALLTSTIQALVFTLLAAVYFSMMLPHEEHEEH"
     gene            complement(1364..1768)
                     /locus_tag="ENO07_01075"
     CDS             complement(1364..1768)
                     /locus_tag="ENO07_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00585.1"
                     /translation="MGLDFINRVIKTTLLLGALVFIFGSYYFDWTYSLGIFTGVLWGC
                     ANLWFIREFIVNYLTTGDRNAGKLALLALIKFPILYGAGFLVLWLGWFPVVSFVAGFS
                     LIFVVVVFKALGLLVMEGGFKNFNLNEKRVEG"
     gene            complement(1777..1959)
                     /locus_tag="ENO07_01080"
     CDS             complement(1777..1959)
                     /locus_tag="ENO07_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HDS00586.1"
                     /translation="MLVAIPFLLAVGPIVGWFIGKYLDEWLGTEPYLMYLFIVFGFIA
                     AGKEVYSLVKRVSEKV"
BASE COUNT          614 a          532 c          430 g          462 t
ORIGIN      
        1 cctcggcatc gatctccttc agcttctctt cgtattcaga gagaagctgc tcggattttt
       61 tacggtcctc ggcggcctcg tcaaaatccg actggatttt ttggcgcctt tcctcaagaa
      121 tatttatgat gggctgccag gcaaatttct tcagaatcca gagtgcaatc agaaaaccga
      181 cggcatgcgt caagagcatt tgccatttgc cgatatcctc cacagtaact cctttgcaaa
      241 gaatttacag atgggatgaa gcgtatggct ccatcccatg cagtaaactt ttcttagtgc
      301 gcaccaattt taccagacag aataaagaag ccgaccagcg aataaatcgt cagagcttcg
      361 atgagggcgg caccgataat cataccgacc tgaatcttgc ccgcggcttc gggctgacga
      421 cccatggctt ccattgctga tccaacggcg cgtcccaaac cgagacccga accgatagca
      481 gccagggcca ggccgattgg caacgaaaac gataaagcag tcgagaaatc catctatcct
      541 cctacggtgt tgtttgaatg ttaatgttct tcatgttcct catgcggcag catcatcgaa
      601 aaatataccg ccgctaaaag cgtaaatacc aacgcctgta tagtcgatgt taataatgcc
      661 aggaagtaaa aaggcagctg caacggcagc cccaccgggg agccgataaa ggccagaacc
      721 atcactccca gacctgtgaa agccgccaga agcacgtctt caccggtgat atttccaaaa
      781 agtcgcaggg agagcgaaag cggtttagcc agctcaccta ttatatgaat cggtaaatta
      841 agaggtacca gcgcccacgt aatggcgctc cggggctcgc ccgcaaggtg gtcaagatac
      901 ctgaggaacc ccatacggcg catgcccgtg tactgcacat acagaaaaac tatgatcgcc
      961 aggggagcgg tggtagccca gtttgctgtt ggagacttgc cgaagggaat cagcccgaac
     1021 cagttcatca ggagaatata catgaacaga gtacccagaa acggggtata gtgcctgccc
     1081 catctgtctc ccagaatcga aataaagaaa ctctggaatc cctccacaat catctccagc
     1141 agattctgca atttcccagg atgcaatgat cttttggcat agaccctcat ggatacagcg
     1201 cacatgaaaa tcagaaccag aaaaccaaaa atcacatcct tgtattcaaa caggaaattg
     1261 acaaacggcc ctgtcaggta gatgtgcaga aattcaatga aatgcggaag ctccggggtt
     1321 cctccctcgt gagcggtaac cagatttgtt ataccaggaa ttatcatccc tcaacccgtt
     1381 tttcatttaa gttaaaattt ttgaagccgc cttccataac aagcagaccc agggccttaa
     1441 aaacaaccac cacaaatatc aggctgaatc cggccacaaa ggaaaccacc gggaaccatc
     1501 ccagccacag gaccagaaaa cccgctccat acaaaattgg aaatttaatc aatgcgagca
     1561 gcgccagttt gccggcattt ctatcgccgg tagtgaggta gtttactata aactctctta
     1621 taaaccagag gttggcacat ccccataata cgcctgtgaa aatgcccagg ctgtaggtcc
     1681 agtcaaaata atagcttccg aatatgaata ccagtgcccc gagaagaagc gttgttttga
     1741 tgactctatt tataaaatca aggcccatag atgcattcaa actttttctg aaactctctt
     1801 aacgaggctg taaacttcct ttcctgcagc gataaaacca aaaactataa agagatacat
     1861 taaataaggt tctgttccta accactcatc cagatatttt ccgataaacc atccgacgat
     1921 cggcccgacc gcaagcagaa acggtattgc gaccagcatc gaaagctgcc cataaaggga
     1981 gactttatct tcttttttct ctggcaccac atccaaaaat taaattgcta atatatac
//
LOCUS       DSEB01000054            2038 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1009678, whole genome shotgun sequence.
ACCESSION   DSEB01000054 DSEB01000000
VERSION     DSEB01000054.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1009678"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(39..1190)
                     /locus_tag="ENO07_01085"
     CDS             complement(39..1190)
                     /locus_tag="ENO07_01085"
                     /inference="COORDINATES: protein motif:HMM:PF13360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00587.1"
                     /translation="MKKIGIYILLLCMAGAVISCSPTFRKFKYINNAPREWSMFRNSL
                     DGYYQKGIYGADINGLKWKAGLKARSYSSPVATESYVAVSALDSYLYFFDLESGDRIN
                     IYKFKSPLSESPLISNKVIYAATGPDKNYLAGLNLITGKYVFREEMKDISSPIIADEK
                     CIYIGDYTGRFVCMDKYAGKIFWEFRTEGPIMNAPAVKDGKVFVGSLDQKLYAFDARS
                     GELLWTHDVGSAIMTAPAVDTLVYFGTFGGEIYAIRADNGNFVWSFKTDGHVISSPVI
                     DDDYVFTGSNDKHLYCLDKENGSEIWSFETDGIINSTPLVMEDAAVIASGDGKLYLLD
                     KLSGELIFSYETENQIKSSPIYFNGNIFVSSLDQHLYCFSKKSPVSANK"
BASE COUNT          553 a          547 c          457 g          481 t
ORIGIN      
        1 atattgcatt aacatctgcc actatgacat tgtggcagtc acttattggc agagaccgga
       61 gatttcttgc tgaagcaata cagatgttga tcgagggagg aaacaaaaat atttccgttg
      121 aagtaaatcg gtgaagattt tatctggttt tcggtctcat acgagaagat caattccccg
      181 ctcagcttat ccagcaggta taatttcccg tcgccgcttg cgattactgc cgcatcctcc
      241 atcaccagcg gtgtcgaatt gataatacca tcggtttcaa aagaccagat ttcagatcca
      301 ttctccttat ccagacaata caaatgcttg tcgttggagc ctgtgaaaac ataatcgtca
      361 tcgatcacgg gagatgagat aacatgcccg tcggttttga atgaccagac aaaattgccg
      421 ttatcggccc tgatcgcata gatttcgccg ccaaaagtcc caaaatacac cagcgtgtcc
      481 acagcgggcg cagtcattat ggctgaaccc acatcatgag tccacaataa ttcccccgag
      541 cgggcatcaa atgcgtacag cttttgatcc agcgatccca caaatacttt tccatccttg
      601 accgcaggcg cattcataat cggcccctcg gtccgaaatt cccagaaaat cttgcctgca
      661 tatttatcca tgcatacgaa acgcccggta tagtctccga tgtagatgca tttctcatca
      721 gcaatgatgg gagaacttat atccttcatc tcttccctga aaacatattt cccggtgatc
      781 agattcaagc ctgccaggta atttttgtcc ggcccggtgg cggcatagat taccttgttg
      841 gagatcagcg ggctttccga aaggggcgac ttgaatttat aaatgttgat cctgtccccc
      901 gattccagat caaagaaata caggtaggaa tccaatgccg acacagccac ataggattcg
      961 gtcgccaccg gtgatgaata gcttctggcc ttcaggccgg ctttccattt cagcccgttg
     1021 atatccgctc catagattcc tttctggtaa tatccgtcca ggctgttacg aaacatgctc
     1081 cactcccggg gggcgttatt gatgtacttg aacttcctga aagtcggcga acaggaaata
     1141 accgcgccgg ccatgcaaag cagtaatatg taaatgccga tcttcttcat cagaaattcc
     1201 acccgaacca gaagtcaaaa tcccagctgg gagagatctt ctcaaaatca gttgtcctcg
     1261 taaaatcgaa cctcagcacc aggaaatatc ccagcgccac acgcgcgccc aggccgaatg
     1321 cgccatagag ctggtcgaac tcatcccccc aggccgaacc cacatcggca aagatcgccc
     1381 cgcgaatccc ggtgaatccg attgccccta caggcaggcc gatatgcagc ctgttgatga
     1441 gcgggaaacg aagctcgttg gaaagcagga aaatattcct cttgtaccac tccctgtggt
     1501 cgaatccgcg caggctccag cttccgccga aatacagcct ctggggctcc ttgcccccgg
     1561 aggtatagta ccaggcccgg gtcgcaaaag cggagtactt gcccagccgg aagtatttgc
     1621 gcaggtccac catgaacagg cggttaaaag cgctgctgct ctttaagtca taggaaaaac
     1681 cggcagttat aatatacctt gaaccatcaa gcggaccggt ataatcccag atcgaattat
     1741 ccttgacata cgacagatag agcgtggtca ggacggcatt ccgaaatgta ccgggaaaga
     1801 agagctcctt ttccgacttg cgcacaaata accttgtctc gaaacgcttg aacttgctga
     1861 aaggatagct gatactgccc ataccgccgt atatgcgctc ggcaaaaagg ccctcggccg
     1921 gatcatcata ttcgtcataa aaatgataca gcccataagc ataattaagc ctgtgctcgg
     1981 tattcaaata ggtggcggcg acattgaaag atgaaaagaa acttgacctt tcctgggc
//
LOCUS       DSEB01000055            3501 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002922, whole genome shotgun sequence.
ACCESSION   DSEB01000055 DSEB01000000
VERSION     DSEB01000055.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002922"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1987)
                     /gene="feoB"
                     /locus_tag="ENO07_01090"
     CDS             complement(<1..1987)
                     /gene="feoB"
                     /locus_tag="ENO07_01090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDS00588.1"
                     /translation="MAKKKIRVALAGNPNSGKTSIFNALTGAHQKVGNYPGVTVEKRV
                     GYASYNGYELEFVDLPGTYSLNAYSTDEKVARDFLIDFKPDVVINVIDSGNLERNLYL
                     TTQLLELECDIIIDLNMWDEAQRMGIHINTSKLSRLLGAPIVTTIGHRGSGTADLLRA
                     AVDLVENRHLDHRHAPVTYGSHIEDLVVDLTRNIEKCKSCELCPSKRWLAVKLLEGDI
                     KTESKFIRDTNELDKLQKSIRKKVRHVKRATGEDPEVILTDGRYGYISGIMKEVVTHK
                     TGDRMRQSRQIDAILTHRFLGYPIFLFLIWLVFQATFVIGNYPMEWIEYGVSILGSAV
                     DNMLPDGFINDLIVDGAIAGSGSVLVFLPNIMILFLGIAALEDSGYMARAAFLMDRLM
                     HALGLHGKSFIPMIMGFGCNVPAIMATRTLESERDRIMTILLIPLMSCSARLAVYVLF
                     AGAFFGAMAGNVIFAIYLLGIAAAVGIAQILRKTLFRQEVAPFVMELPPYRIPTIKSV
                     VIHMWERASIYLKKIGGIILIASVIIWLLGYIPKPEEYSQDYGTRIENLQLQLERAAA
                     SEQEEQQIRQELSDLQAGKSSEEIQFSYIGRIGKGVEPAIEPLGFDWRLGVSLITGFV
                     AKEIVISTLGVLYQIGEEETEESTGLTDALRDPRSNIT"
     gene            complement(1980..2705)
                     /locus_tag="ENO07_01095"
     CDS             complement(1980..2705)
                     /locus_tag="ENO07_01095"
                     /inference="COORDINATES: protein
                     motif:HMM:PF01325.17,HMM:PF04023.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDS00589.1"
                     /translation="MKISSKIEDYLETIYRLSKEMDTVGVTDVAKARNVSVPTARTAV
                     NRLQEYGFLHQKHYGKIILHEAGKKKGEEIYRVHKTLRRFLRDFLLVDEDTAEKEACK
                     MEHGLSHETLERLQIFLRTLEESEVCEKRCMNKYEESLKHLNLENAGSEGTKSSDSSS
                     QRELKTMTLLDLKPGETGIISKVGGYGKLRHRILDLGLHVGERIAMVKTAPLKDPIEF
                     SLNGNHISLRRTEAELIQIKSNG"
     gene            3061..>3501
                     /locus_tag="ENO07_01100"
     CDS             3061..>3501
                     /locus_tag="ENO07_01100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDS00590.1"
                     /translation="MKVKTPAEIKAVDLQVDKITCQGCVNFIEKTISDIDGVTTVHAN
                     LAESSVNVHYEPGRIDLNSIRRRLDDINYNVRTSRASIQIEGMHCASCVSKIENEVSR
                     LEGVVNIRVNLADQTARVEYIQQIIDPGTILKKIESLGYRASQQE"
BASE COUNT          877 a          852 c          827 g          945 t
ORIGIN      
        1 gagtgatatt gcttctggga tctctgagcg catcggtcag tcctgttgat tcttcggttt
       61 cctcctctcc aatctgataa aggacaccga gagtggatat cacgatttcc ttggccacaa
      121 atccggtgat gagcgaaacg cccagcctcc agtcgaatcc caaaggctcg atagccggtt
      181 ctacgccctt gccgattcgg ccgatgtaag aaaactgtat ttcctcggag gacttgccgg
      241 cttgaaggtc gctaagttct tgcctgattt gctgctcttc ctgttctgag gcagctgccc
      301 gttccagttg caattgcagg ttttctattc gggttccata atcctgcgag tattcttcag
      361 gttttggtat atatccaaga agccatataa ttactgatgc aatcaggatg atcccgccga
      421 tttttttcag atatatggat gccctttccc acatatgtat gacaacgctc tttatagttg
      481 gtatcctgta cggcggaagt tccatgacga agggcgcaac ttcctgcctg aaaagggttt
      541 tgcgtagaat ctgggcgatc ccgacggctg ccgcgatgcc caggagatat atggcaaaga
      601 tgacattacc tgccatggcg ccaaagaaag cgccggcaaa gagaacataa accgccagcc
      661 tcgcgctgca tgacataagc ggaatcagga ggattgtcat aatccggtca cgctccgatt
      721 ccagggtacg ggtggccatt atagcgggga cattacatcc gaagcccatt atcatgggaa
      781 tgaaggactt gccgtgaagg cccaaggcat gcatcagcct gtccatcaga aatgcggccc
      841 gggccatgta acccgagtcc tccagagccg ctattcccag aaacaggatc atgatattgg
      901 gcagaaacac cagaacagag ccgcttcccg caatggcgcc atccacgatc agatcgttaa
      961 taaagccgtc cggcagcata ttgtccaccg cactccccag aatggaaacc ccatattcga
     1021 tccattccat aggataattt ccaattacaa acgtggcctg gaaaaccagc cagatcagga
     1081 agagaaagat gggatagcca aggaaccggt gagtcagtat ggcatcaatc tggcgcgact
     1141 gcctcatccg gtcgccggtt ttgtgggtga caacctcttt catgattccg gagatgtagc
     1201 catagcgtcc atcagtcagg ataacctcgg ggtcctcgcc tgtggcgcgc ttgacatgac
     1261 gaaccttctt gcggatactt ttctgcaatt tatccagctc atttgtgtct cttatgaatt
     1321 tcgattcggt cttgatatcc ccttccagga gcttaaccgc cagccatcgc ttgcttggac
     1381 agagttcaca gcttttacat ttttctatgt ttcgggtcag atcgaccacc agatcctcga
     1441 tatgcgaacc ataggtaacc ggggcgtggc gatggtccag gtgcctgttt tcaacgagat
     1501 ccacggcagc ccgcagtaaa tctgccgtac cgctgccgcg atgtccgatg gtggtcacga
     1561 tcggcgctcc cagaagtcgg gagagtttgg aggtatttat atggatgccc atcctctgtg
     1621 cttcatccca catattcaga tcgatgatga tatcgcattc cagctccaga agctgggtgg
     1681 tgagatagag atttctttcc aggttgccgc tgtcgatcac attgatgaca acatccggct
     1741 taaaatctat gagaaaatcc cgggcgacct tttcatctgt ggaatacgca ttcaggctgt
     1801 aagtgccggg aaggtcgaca aattcgagtt cgtaaccgtt ataacttgcg tatccgacac
     1861 gtttttcaac tgtaactccg ggatagttgc cgaccttttg atgggctccg gtgagggcgt
     1921 tgaaaataga ggttttgccg gaattgggat ttcccgctaa ggcgactcgg atttttttct
     1981 tagccattgg atttgatctg gatcagctcg gcttcggtgc gcctgaggct gatatgattg
     2041 ccatttaaag aaaactcaat gggatcctta agaggagccg ttttaaccat ggcaattctt
     2101 tcgcccacat gcagacccag gtccaggata cgatggcgca gttttccgta tccgccaacc
     2161 ttcgatatta tgccggtttc tcccggcttc aaatccagta aagtcatggt ttttaactcc
     2221 cgctgagatg aggaatcgct gctcttcgta ccttccgatc cggcattttc cagattcaga
     2281 tgcttcagag attcctcgta cttgttcata caacgctttt cgcagacttc gctttcttcc
     2341 agtgtcctca aaaaaatctg aagccgttcc agggtctcat ggctaaggcc atgttccatt
     2401 ttgcaggctt ccttttccgc cgtatcctca tccacaagca aaaaatccct gagaaagcgc
     2461 cgcagggttt tgtgtactct gtagatttcc tcgcctttct tttttccggc ctcgtgcaga
     2521 attatcttgc cgtaatgttt ctggtgcagg aagccatact cctgcaggcg gttaaccgct
     2581 gtgcgggccg tgggtaccga gacatttcgg gccttggcaa catcggtaac tccaaccgta
     2641 tccatctctt tggaaaggcg gtatatagtt tccagataat cctctatttt cgatgatatt
     2701 ttcatatggc tcttacttta taagattata cttactttgt caatattatg cttgaaaatt
     2761 cgtaaaaaat attcgaaaat ccacttccgg atttatcgtt ttaagaagac ggatcatctt
     2821 atatcatgta tcgcaacaga ttagcagaaa gcgccgaatt agtttcgcct gaccgatatt
     2881 tatttaaatt aagcttgcaa aaataaggga aaatgattga ttatttgaca agtatttctt
     2941 actgaattca ccaaactgtg ccgaatctat ggatagtagt cagcactaaa tttctctgga
     3001 acaaattttg tataatatgg cttagttaga gccgaagggt catttttata agcaggaaat
     3061 atgaaagtaa aaacaccggc agagataaaa gctgtagatc ttcaggtaga taaaatcaca
     3121 tgccagggct gcgtcaattt catcgaaaag acaatttctg atatagatgg tgtcaccact
     3181 gtgcatgcta atctggcgga gagctctgtc aatgtgcatt atgagccggg caggatcgac
     3241 ctgaattcga tccgccggcg tctggatgat atcaactaca atgtcaggac cagccgggcc
     3301 agtatccaga tagaggggat gcattgcgcc tcatgcgtgt caaagatcga aaatgaggta
     3361 tctcgtcttg agggtgtagt caatatccgc gtaaatcttg ccgatcagac cgccagagtt
     3421 gagtacatac aacagatcat agatcccggg acaattctga agaagatcga atccctggga
     3481 tacagggcct cccaacagga a
//
LOCUS       DSEB01000056            2226 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1007985, whole genome shotgun sequence.
ACCESSION   DSEB01000056 DSEB01000000
VERSION     DSEB01000056.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1007985"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            19..1704
                     /gene="hutU"
                     /locus_tag="ENO07_01105"
     CDS             19..1704
                     /gene="hutU"
                     /locus_tag="ENO07_01105"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HDS00591.1"
                     /translation="MSDTSTKTPAEYTPVKADRGSSLSCKNWHAEAAMRMLKNNLDPE
                     VAEKPEELVVYGGSGKAARNWDCFHAIVKSLKELKNDETLLVQSGKPVGIFRTFEHCP
                     RVLIANSHLVPHWATWEKFRELEKLGLIMFGQMTAGSWIYIGTQGILQGTYETFAECG
                     RQHFGGDLSGKFILTGGYGGMGGAQPLAATMAGAIMLGVEVDEARIDKRLDTGYCDRK
                     THSLEQALQWVEEAKRSKVPLSIGLVGNAAEIEPKMVEMGVVPDIVTDQTSAHDELNG
                     YIPHGMPYEEALELRKKNPDEYIKRAYDSMVAHTQAMLDFQKAGSIVFDYGNNLRGQA
                     QKAGLRNAFDFPGFVPAYIRPLFCEGKGPFRWAALSGDPKDIHVTDEVILKEFSYNES
                     LCNWIKMAHEKVHFQGLPTRICWLGYGEREKFGLIINDLVRKGKISAPIVIGRDHLDC
                     GSVASPYRETESMKDGSDAIADWPLLNAMLNICAGASWVSIHHGGGVGIGLAIHAGMV
                     VVCDGSKEMDERLSRVLTTDPGSGIMRHADAGYEKAIEVARKKGVKLPMIRDE"
     gene            1930..>2226
                     /locus_tag="ENO07_01110"
     CDS             1930..>2226
                     /locus_tag="ENO07_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00592.1"
                     /translation="MYYLGDDAILKGEKAVILNSRQSKTPVGSDPWIQNSYKAVEDAI
                     SRGCAIVTSIGMNTWEFLVWAAGHLGGKQVIVAPLDEREDQEEIRRDIIEDFELD"
BASE COUNT          550 a          501 c          648 g          527 t
ORIGIN      
        1 aataaaggag tttatgctat gtcagataca tctacaaaaa cgccagcaga atacactccc
       61 gttaaagccg accgtggcag cagcttgagc tgcaagaact ggcatgccga ggcagccatg
      121 cgcatgctca aaaacaacct cgacccagag gtggcagaga agccggaaga gcttgtggtt
      181 tacggcggtt ccgggaaagc agcgcggaac tgggactgct ttcatgcgat agttaagagc
      241 ctgaaggaat tgaagaatga cgagactctc ctggttcagt ccggcaagcc ggtgggaatt
      301 tttcgcacat tcgagcactg cccccgcgtg ctgatcgcca attcgcatct ggtaccgcac
      361 tgggcgacgt gggaaaaatt ccgcgaactg gaaaagctgg gcctgattat gttcgggcag
      421 atgacagccg gaagctggat ttatatcggt actcagggaa tactccaggg tacatacgag
      481 acctttgctg aatgcgggcg ccagcatttt ggcggcgatt tgagcggaaa atttatcctg
      541 accggaggat atggcggtat gggcggcgcc cagccgctgg ccgctactat ggccggggcc
      601 attatgcttg gtgttgaggt agatgaggct cgtatcgata agcgccttga taccggttac
      661 tgcgaccgca agacccacag cctggaacag gccctgcaat gggtcgagga ggccaagagg
      721 tccaaagttc cgctatccat cgggctggtc ggcaacgctg ccgagatcga gccgaagatg
      781 gtcgagatgg gcgtggtgcc tgatattgtg accgaccaga cctcggccca tgatgagctc
      841 aacggttata ttccgcatgg aatgccgtat gaagaggctc tggaattgcg aaagaagaat
      901 ccggatgaat atataaagcg tgcttatgat tctatggttg cgcacaccca ggcgatgctc
      961 gatttccaga aagccgggtc gattgtcttt gattacggca ataatcttcg tggccaggcg
     1021 cagaaggcag gtctcagaaa tgctttcgat tttcccggat ttgtgccggc ttatatccgt
     1081 ccgcttttct gtgagggcaa aggacccttc agatgggcgg cgctttccgg cgatccgaag
     1141 gatattcatg taacagatga agttattctg aaagaatttt cctacaatga atcgctttgc
     1201 aattggatca agatggccca tgagaaggtg catttccagg ggctgccgac acgaatctgc
     1261 tggcttggtt acggcgagcg tgagaagttc ggcctgatta taaacgatct ggtgcgcaag
     1321 ggcaaaatct ctgccccgat cgtaatcggc cgagatcatc tcgactgcgg ttctgtggcc
     1381 tcgccctatc gtgagaccga atccatgaag gatggttccg atgccatcgc cgactggccg
     1441 cttctgaatg ccatgctgaa tatctgtgcc ggggcatcct gggtctcaat ccaccacggc
     1501 ggcggagttg gaattggcct ggcgattcat gccggcatgg ttgtggtttg tgacggctcg
     1561 aaggagatgg acgagagact ctcacgggta cttacaaccg atcccggttc cgggatcatg
     1621 cgtcatgccg acgcaggata cgagaaggcg atagaggtgg cgcgaaagaa gggtgtgaaa
     1681 ctgcctatga tacgggatga ataggctgga atagaattgt ttattgaacg cgtgagagaa
     1741 tcttaatctt tcgcgcgttt tttatttcat ttattcaatt tttgaaaatt tgattttgta
     1801 tagacgcttt tttggcttat attgtcatct cagaaattga attatcagaa gcccatggct
     1861 gcttatttat gaaataccac aagtaggggc ttggaaaatt ggacttacaa catttgagcg
     1921 ggcttaaaaa tgtattattt gggggatgat gctatcctaa aaggggagaa ggcggttatc
     1981 ctgaactccc gacaatcaaa gactccggtt gggtctgatc catggatcca gaacagctat
     2041 aaagcagttg aggacgccat atcccggggc tgcgccatcg tcacctcgat tggcatgaac
     2101 acgtgggaat ttctggtctg ggcagccgga catcttggag gaaaacaggt catagtggcc
     2161 ccattggatg aaagagagga tcaggaggag atcaggcgtg atatcataga ggactttgag
     2221 ctcgac
//
LOCUS       DSEB01000057            2226 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1007983, whole genome shotgun sequence.
ACCESSION   DSEB01000057 DSEB01000000
VERSION     DSEB01000057.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1007983"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..608)
                     /locus_tag="ENO07_01115"
     CDS             complement(<1..608)
                     /locus_tag="ENO07_01115"
                     /inference="COORDINATES: protein motif:HMM:PF02861.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDS00593.1"
                     /translation="MNEMFTELARKAIEYARDEATRLRHDYIGTEHLLLGLIRLGEGR
                     SVDIITNIGLELQDLKQSIEDVVQPSGGTMTMGQLPLTARAKKTLEVAGQEARALKSK
                     DIDTEHILLALLKDEEGVAAQVLSMYDISYQEAYEELKNIQAGKPSNFAKKRKKSKTP
                     ALDHFGRDLTELARRGKLDPIIGRVDEIERVTQILSRRKKNNP"
     gene            complement(632..1684)
                     /locus_tag="ENO07_01120"
     CDS             complement(632..1684)
                     /locus_tag="ENO07_01120"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: protein motif:HMM:PRK01059.1-4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HDS00594.1"
                     /translation="MFEDIIHKTAAWLRGDGTEPAVVVSSRVRLARNIQNILYPNKGS
                     AQEMEDAREFVHSALRKENMLAKGIYVQSEDMTELDRRFLIERHLISPEFRATKPSRG
                     IYLSNDEDISIMINEEDHIRIQSLTSGLNIHRAMEIAEEVDDTLGNTLAYDYDTDYGF
                     LTSCPTNCGTGMRASVLIHLPGLVLTHEIDKVIEEITKVGLAVRGFYGEGTEVLGNLF
                     QISNQTTLGRREKDITDSLEQITRQLIEHEENARRILVRDAPDEIKDKIWRAYGILKY
                     ARVLTSEETMNLLSAVRLGMALGVLDMIPLTLLNEILVQAQPAHIQKVAGKNVSPSER
                     DAKRAELIRARLSELK"
     gene            complement(1694..2194)
                     /locus_tag="ENO07_01125"
     CDS             complement(1694..2194)
                     /locus_tag="ENO07_01125"
                     /inference="COORDINATES: protein motif:HMM:PF02151.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00595.1"
                     /translation="MLCDNCKKNEATINFTQVANDKKIEYHLCESCAAKLGFKNPLKA
                     VPFPLGDFLSSMVDKDIKEKTGSLVQINCPRCGLTFAEFSKTGKFGCGKCYRAFKTQL
                     DDLLRKIHGSNRHEGKLPRGSSESMEPLRKERELQEKLRDAIKNEEFEKAAELRDQLR
                     FLMENK"
BASE COUNT          496 a          601 c          508 g          621 t
ORIGIN      
        1 gggttgttct tcttgcgccg agaaagaatc tgtgtcaccc gctctatctc atcgactctg
       61 cctatgatcg gatcgagctt gccgcggcgg gcaagttcgg tcaggtcgcg tccgaaatga
      121 tccagcgccg gggtcttcga ctttttgcgc ttcttggcaa aattcgaggg cttgccggcc
      181 tgtatattct tcaactcttc ataggcttcc tgataggaaa tgtcatacat cgataagacc
      241 tgagcggcca cgccctcttc atctttcaag agcgcgagca gtatatgctc ggtatcgatg
      301 tctttcgact tcagggcgcg tgcctcctgt cccgcaactt ccagggtctt cttggccctg
      361 gcggtcaaag gaagctggcc catagtcata gttccgcctg agggctggac tacatcctcg
      421 atggactgct ttagatcctg aagttctaat ccgatgtttg tgatgatgtc gacagatcgt
      481 ccctcaccca gacggattag tcccaataga agatgttccg taccgatgta atcgtgccgc
      541 aaacgtgttg cctcatcccg cgcgtattca attgccttgc gggccaattc ggtgaacatc
      601 tcattcataa taaaaatcct ccatgtgtaa gctatttaag ctcggacagc cttgccctta
      661 tcaactcggc ccgcttcgca tcccgttccg atggggaaac atttttcccg gccactttct
      721 gtatatgcgc aggctgagcc tgaaccagga tttcgttcaa aagcgtcaga ggtatcatat
      781 ccagaacgcc cagcgccatt ccaagtcgca cagctgacaa taaattcata gtctcctctg
      841 atgtcaatac ccgagcgtat tttaaaattc cgtaagcgcg ccatatctta tcctttatct
      901 catcaggggc gtctcgaaca aggatacggc gtgcattctc ctcatgttct attaactgtc
      961 gcgtaatttg ttccagagaa tcagttatat ccttctccct gcgccccagt gtcgtctggt
     1021 tcgatatttg aaacaaattt ccaagcactt ctgttccttc tccgtagaat ccgcgcacag
     1081 ccagccccac tttcgtaatt tcctcgatca ctttatcgat ttcgtgggtc aggaccagcc
     1141 cgggaagatg aatcagcacc gaagcgcgca taccggtgcc gcaattcgtg gggcaggagg
     1201 tcaggaatcc gtagtcagtg tcataatcat aggccagcgt atttcccaga gtatcgtcaa
     1261 cttcttcggc gatttccatc gccctgtgaa tattcaaacc ggaagtaagc gactggattc
     1321 tgatgtgatc ctcttcattt atcattatgc tgatatcttc gtcatttgac agatatatcc
     1381 cccttgaggg cttggtggcc cggaactcgg gggagatcag atgtctctcg atcaggaatc
     1441 tgcgatccaa ttcggtcata tcttccgatt gaacataaat gcctttcgcg agcatgtttt
     1501 ccttgcgcaa agcggaatgc acaaactccc gggcatcctc catttcctgg gcgctacctt
     1561 tattaggata aagaatattc tgaatatttc ttgccaggcg gaccctgctt gagaccacta
     1621 ccgcaggttc agtaccgtca cctctaagcc aggcggccgt cttgtgtata atatcttcga
     1681 acatagcctg ctcctacttg ttctccatca aaaacctgag ttggtcgcgc aactcggcag
     1741 ccttttcaaa ctcttcgttc ttgatagcat ctcggagttt ttcctgcaat tcgcgctctt
     1801 tgcgcaaagg ttccatggat tcagagcttc cccggggaag tttgccctca tggcggttgg
     1861 aaccgtggat cttcctcagc aggtcgtcaa gctgagtctt gaaagcccga tagcatttac
     1921 cacagccgaa tttgcctgtc ttggaaaact cggcaaaggt cagaccgcag cgggggcagt
     1981 taatctgcac cagtgagccg gtcttctcct ttatatcttt atcaaccata gatgacaaaa
     2041 aatcacccag cgggaagggc acggccttga gaggattttt gaatccgagc ttggcagcgc
     2101 agctttcgca gaggtgatat tcgattttct tgtcgttcgc cacctgggtg aaatttatgg
     2161 ttgcttcatt ttttttgcaa ttgtcacaaa gcataactcc gaaacctccg ctactgtaaa
     2221 tgcaac
//
LOCUS       DSEB01000058            4127 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002035, whole genome shotgun sequence.
ACCESSION   DSEB01000058 DSEB01000000
VERSION     DSEB01000058.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4127)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002035"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            500..955
                     /locus_tag="ENO07_01130"
     CDS             500..955
                     /locus_tag="ENO07_01130"
                     /inference="COORDINATES: protein motif:HMM:PF06445.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDS00596.1"
                     /translation="MAEHEVRIIKMPACRLALVNTVSSSPEMESIQKLMKWVEKRNLA
                     DREYGPRWFGRNNPPPQDDKAEYGYDAMVTIPDDITNADEVQLYQIPERTCAVVKANL
                     QNITQMWNYLYDWVKESDYEIADHGLEELLDPYANDEEFLFDLWLPVRK"
     gene            complement(988..4077)
                     /locus_tag="ENO07_01135"
     CDS             complement(988..4077)
                     /locus_tag="ENO07_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDS00597.1"
                     /translation="MNITRTAVKRPVATAMVFLIIIVLGIMGFRFLPVDLLPQIEYPM
                     LSISTSYPNVGPEEIETIITDRVENAVAVVPNLTEVRSNSEEGRSWVRLEFAQGTDID
                     AAANDVRAALDRIRDDFPPEVEPPRLWKFDPDNFPVVILGAHSDRPMDELTLVLEREI
                     AQRFEQIPGVGSIDVWGGLYREVQVRLKRDRLASSGLSAADVRAALQRENVTLPGGDM
                     REGISDMYVRTRGEYQSLEQITNTIITVVDGYPIRVGDVAEVVDGFEDINRIVQIDGQ
                     PMVRLGIRKQSGANTVEVARQAREVMERIDRERDDLDLVMVIDQSEFIQNSIDTVKQS
                     ALFGGLLAIFVLYIFLRNGSTTFIIALSIPISLIATFGLLYFNNLTLNQMSFGGLALG
                     IGLIVDNSIVVLENIVRLREGGHSREESALTGTKQVTGAIIASTLTTSVIFLPVVFMQ
                     TISGMLLRELALVVVFALLCSLLVALTLVPMLASRFLTIKSGGGNRNGIRKRLGNWFQ
                     RLEERYAGLLDKALGHKKIVFGVTAVLLAGAILLWPLLPVELAPQTDADEIDVDLMMA
                     QGTNIAVVNEYLYELEQIVREVTPMDEVLHITTQIRPGNAEVELSLKSAGERSINSFD
                     LADQLRREVSGQIPGADIRVSAQSGLWMLRRLFGSGGAEAVQIELRGYDLDVANRLAN
                     QIKNVAERVPEVADVRISRREGRPEQNLVIDREKIADLGLTVNQIAQVIQTNIGGSRA
                     GVFREGGEEFPITVRLQPQDRLTTVDLSNVGVRTAGGDIVPVSAVVETERRRSPTEIE
                     RIDGQRVTYITANLESGAALGDVIGKLQRGMRNVPLPEGFSIVFSGEYEEQQKARNDF
                     LLSVLMAAILIYMVMAAQFERFLDPLIVMIAVPMAIIGVVPTLLLTGTTINIQSLMGI
                     VMLMGIVVNNAIVMVDYINLMRRERGLDINDAVRQSARLRLRPILMTTFTTVLGMLPL
                     AFVLGAGGEIQAALARAVIGGLTVSALITLILIPVTYNAVYNLLERARRLRAAPATE"
BASE COUNT         1083 a         1182 c          963 g          899 t
ORIGIN      
        1 ggataaaatc ggcggagcat tcggatcgta cggctggtca gataaggcta ccaagattct
       61 gaaagaatac atggatgacc tcaaattcga tatcatcgga gacggcctca gcacacaata
      121 tgtccccaca gatgatgtct taaaccaatg ctacgaatac ggcgtcgaaa tcggcaaagc
      181 tgtaaatgcg ggatagagat atagccgtct ggaaagggtt cctgacggca ctcgttaatt
      241 atgtttatca tcgagagcca tgcataacgg tcaaacatac ctcatacgat atcgaatgat
      301 gtcattctca aaataattgc ccaatcacac aaagcaatca caatttcccg gcaaactttc
      361 cgtacagcgg cgtcctgatt tggatttttc ggaattgtaa tcccggaatt acagcatgaa
      421 atcaaaaaaa ggaaatcatt tcgtttacga tatcgtagag taatataaaa aataaatctt
      481 caaaatcgga ggcacaagaa tggcagaaca tgaagtacgt attatcaaaa tgcctgcatg
      541 ccgactggcg ctggtgaata ctgtgagcag cagcccggag atggaatcaa ttcagaagct
      601 catgaaatgg gtcgagaaaa gaaatttggc tgatagagaa tatggtccga gatggttcgg
      661 caggaataat cccccaccgc aagatgataa agcagaatat ggttatgatg ctatggtaac
      721 gatccctgat gatattacta atgctgatga agtccagctc taccagattc cggaacggac
      781 atgcgccgtc gttaaagcca atttgcagaa catcacacag atgtggaact atctttatga
      841 ctgggttaaa gaaagcgact atgaaatagc cgatcacggt ctggaggaac ttctggatcc
      901 atatgcgaat gatgaggaat ttctcttcga cctatggctg ccggtacgaa aataatcaaa
      961 ctcaaaaagc ggaaattgcc gataagccta ttccgtcgca ggtgctgccc tcaacctgcg
     1021 ggcacgctcc agcaaattat agacggcgtt ataagtaact ggaatcaaaa tcagggtgat
     1081 cagtgctgaa actgtcaaac cgccaatgac cgccctcgcc agagccgcct gaatctcgcc
     1141 tccggcgcca agcacaaagg ccagcggaag catacccagc actgttgtaa aggtagtcat
     1201 caaaatcggc cgcagacgca accgtgcaga ctgccgcaca gcatcattga tatcaagacc
     1261 gcgctcccgg cgcatcaggt ttatataatc aaccatcaca atagcgttat tgacaacgat
     1321 gcccatcagc ataacaatgc ccatcaggct ttgtatgttg atggtcgtgc ccgtcaaaag
     1381 cagcgtgggc acaacaccta tgatggccat tggaaccgcg atcatcacga taagcggatc
     1441 aagaaatctt tcaaactgcg ccgccataac catgtaaatc aaaatcgcag ccatcaagac
     1501 cgacaacaga aaatcgttcc tggctttctg ctgctcctcg tactcgccgc tgaatacgat
     1561 cgagaatcct tccggcagag gcacgttgcg catgcccctc tgcagtttcc cgatcacatc
     1621 tcccagcgcc gctccgcttt ccagattagc agtgatatag gtcacccgct gcccgtcaat
     1681 gcgttcgatt tctgtcgggc tgcgcctccg ctcagtttcc accactgcag agaccggcac
     1741 gatgtcgccg cccgcagttc gcacacccac attgcttaag tccacggttg tcagtcggtc
     1801 ttgcggctga agccgaaccg tgataggaaa ttcctctccg ccctcacgga acacccctgc
     1861 ccggcttccg ccgatattgg tctgaattac ctgtgctatc tggtttaccg tcaatcccag
     1921 gtctgcaatt ttctcacgat cgatgaccag gttctgctcg ggtcttcctt cccgcctgct
     1981 gatccggaca tccgccacct ccgggactct ttcagctaca ttctttattt gattggccag
     2041 gcggttggcc acatccagat cgtatccccg cagttcaatc tgtacagctt cagcgccccc
     2101 ggacccaaac agccgccgca gcatccacag ccccgactgc gccgagacac ggatatccgc
     2161 cccgggaatc tgtcccgaga cttcacggcg aagctggtcg gccaaatcaa aactgttgat
     2221 ggatcgttct ccggcactct tgagcgagag ttccacctcg gcatttccgg gacgaatctg
     2281 agtggttata tgaagcacct catccatcgg cgtaacctcc cgcactatct gctccagctc
     2341 atacagatac tcgttcacca cagcgatgtt cgtcccctgg gccatcatca ggtccacatc
     2401 aatttcatcg gcatcggttt gcggagccag ttccaccggc aacagcggcc acaacaatat
     2461 agcgccggcc aacagcactg cggttactcc aaagacaatt ttcttatgcc ccagggcctt
     2521 atccagaaga cccgcgtatc gctcctcgag ccgctgaaac caattgccga ggcgcttgcg
     2581 aataccattg cgatttccac cgccggattt gatcgttaaa aatcggctgg caagcattgg
     2641 caccagggtg agcgccacca ggagcgaaca cagaagggca aataccacca ccagcgcaag
     2701 ctcgcgtaaa agcataccgg agatagtctg catgaaaact accggcagaa agatgactga
     2761 ggtggtgagt gtcgatgcta taatggcgcc ggtcacttgc tttgtaccgg tgagagcgct
     2821 ttcttcacgg gagtgaccgc cctccctcag gcgaacgata ttttccagga ccacaatgga
     2881 attgtcgaca atcaatccaa tccccagcgc cagcccgcca aaactcatct ggtttaatgt
     2941 caggttattg aaatacagaa gaccgaaggt agcaatgagc gagatcggga tcgagagggc
     3001 aatgataaat gtagtcgagc cgtttcgcaa aaagatataa agtacgaaaa ttgccaggag
     3061 cccgccgaaa agtgctgact gcttcacggt atcaatcgag ttctgtatga attcgctctg
     3121 gtcgatcacc atcaccagat ccagatcatc ccgctcccgg tcaattctct ccatcacttc
     3181 ccgcgcctgt cgggccacct cgaccgtgtt ggcgcccgac tgcttgcgaa tccccagccg
     3241 caccatgggc tggccgtcaa tctgcacgat gcggttgatg tcctcaaaac catccaccac
     3301 ctcggccaca tcaccgaccc gaatcggata gccgtcaact accgttatga tcgtattggt
     3361 tatctgctca agcgactgat attctccccg agtacgcaca tacatatcgc ttatgccctc
     3421 tcgcatgtcg ccgccgggaa gagtgacatt ctcacgttgc agtgccgcac gcacatcggc
     3481 cgcggagagg ccgctggagg ccagacggtc gcgtttgagc cgcacctgca cctctcgata
     3541 cagaccgccc cagacatcta tcgaaccgac acccggaatc tgctcgaaac gctgggcaat
     3601 ctcgcgctcc aaaacgagcg tcaactcgtc cataggacga tccgaatgtg ctcccaggat
     3661 tacgaccgga aaattgtcgg gatcaaattt ccataaacgc ggcggctcga cttccggcgg
     3721 aaaatcatcg cgaatccgat ccagcgccgc ccggacatca ttggccgcag cgtcaatatc
     3781 cgtcccctgc gcaaattcga gtctgaccca gctgcgccct tcctcagaat tcgagcgcac
     3841 ctcagtcagg ttgggtacca cagcgacagc attttccaca cggtcggtga tgattgtctc
     3901 gatttcctcc gggcccacat tgggataact ggttgagatg ctgagcatgg gatactcgat
     3961 ctgaggcaac aggtcgaccg gcaaaaatct gaatcccatt ataccgagga caataataat
     4021 tagaaacacc atagccgtcg ccacgggacg ttttaccgct gtcctggtaa tgttcatgca
     4081 gtatcctttg aacctctttc cagcataccg accgaagacg tatcaac
//
LOCUS       DSEB01000059            2530 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1006009, whole genome shotgun sequence.
ACCESSION   DSEB01000059 DSEB01000000
VERSION     DSEB01000059.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1006009"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..275)
                     /locus_tag="ENO07_01140"
     CDS             complement(<1..275)
                     /locus_tag="ENO07_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase DegT"
                     /protein_id="HDS00598.1"
                     /translation="MLDLVAPAGTPVPFSFLLKSLFPLPDSKERGDSFIDKIQQEYSI
                     EYCYLLSTGRGALYFILQAAKQLADPEKREVIIPAYTCFSVPSAIVKA"
     gene            complement(321..1388)
                     /locus_tag="ENO07_01145"
     CDS             complement(321..1388)
                     /locus_tag="ENO07_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family PEP-CTERM system-associated
                     protein"
                     /protein_id="HDS00599.1"
                     /translation="MNLIRAADRYIIMLTIREYTAQSKDVWSNFVARCPDSNIGHRIE
                     WKEVMEESFGRRTRYLLALDGDEIRGILPLVMVRTFWGAKALVSMPWIDYGGVCAIDD
                     AGTGMLLQKAREIAEQEKAGFIELRSVQARDKNLADRHDKVTFLLDTNRSPETIWKEF
                     NAKLRNQIRKAHKSGLSTEFTGVEGLDRFYKVFCRNMRDLGTPVWGRNCFEKVLTAFG
                     KDARLILVAKGSKTIAGGVVLSFKDRVYVPWASAIRDWIKYCPNHALYWAVIKDTSEK
                     GYDYFDFGRSTIDSNTFRFKKHWVPVPQQLNWQYYLRTAKDIPSISPDNPAFKIFVKL
                     WQRLPLPIANYFGPKVNKMLP"
     gene            complement(1430..2452)
                     /locus_tag="ENO07_01150"
     CDS             complement(1430..2452)
                     /locus_tag="ENO07_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00600.1"
                     /translation="MKCRKAIKISAIVLGVIVVLAFAFSLYLTPGYDRISFPDGKKFA
                     FSICDDTDASTLENVSPIYRLLDSLGIRTTKTVWVFPSTSEAFPDQGQTLGDSVYLDF
                     VLGLRDRGFEIASHGARGGSSKRDETIRSLHVFDSLIGYYPRIYINHYHNAENLYWGV
                     YRLDYWPLRMLYNLTRDDVDYVGHLPETEFFWGDIAKEYIEYVPNFSFYEINLRKVNP
                     DIPYHDPKRPYVNFWFHTSDGMDVHAFNDLLSEENLDKLEEEGGVCIVYTHFAYDFCR
                     NGEINDTTVERLKDLASREGWFVPASEILDYLREIEKGGNKLSLRQRLYLQFRWIKEK
                     IVYGSS"
BASE COUNT          651 a          644 c          529 g          706 t
ORIGIN      
        1 gctttcacaa tagcggatgg caccgagaag catgtgtatg ccggaatgat tacctctcgt
       61 ttttccgggt cggccagctg ttttgcagcc tgcagtatga aatagagcgc gccccgcccc
      121 gtgctcagaa gataacaata ctctatgcta tattcttgct ggattttatc gataaagcta
      181 tcccctcgct cctttgagtc gggcaaagga aaaagtgatt tcagcaagaa gctgaaggga
      241 accggggttc cggctggcgc taccaaatca agcatttctg aatccagtgt tatttttaat
      301 caagtactgg ttatttgatc ctaaggcaac attttattca ccttgggtcc aaaataattt
      361 gcgatcggca agggcagcct ctgccagagc ttcacaaaaa tcttaaaggc cggattatcc
      421 ggacttattg atggaatatc tttagccgtt ctcaaataat attgccagtt aagctgttgg
      481 ggcacgggaa cccagtgttt cttgaacctg aaggtattgc tgtcaattgt cgaccttccg
      541 aaatcaaaat aatcgtatcc cttctcgctg gtatccttga taactgccca gtaaagggcg
      601 tgatttggac aatacttaat ccaatccctt atcgcggagg cccagggtac atagacacgg
      661 tccttgaagg aaagcacaac tccgcctgca atagtctttg accccttagc aaccaggatc
      721 agtctggcat ccttgccgaa agcggtcagg actttctcaa aacaattgcg tccccagacc
      781 ggcgtaccca gatctctcat atttctacaa aagaccttat agaacctatc aagcccttcg
      841 acgccggtaa actctgtcga cagtccgctt ttatgagcct ttctgatttg attacgcaac
      901 ttggcgttaa attccttcca gattgtttcc ggggatctgt tcgtatcgag cagaaaggtg
      961 actttatcat gccggtccgc aagattcttg tcgcgtgcct gcaccgatcg aagctcaata
     1021 aagccggcct tttcctgctc tgcaatctcc ctggccttct gcagcagcat ccctgtgcct
     1081 gcatcatcaa tggcacatac accgccgtaa tcaatccagg gcatagagac cagcgctttt
     1141 gctccccaga aggttctgac cataaccagg ggcagtatac ctcttatttc atcaccgtcc
     1201 agcgccagaa gatatcttgt ccgccggcca aagctttcct ccatcacttc cttccactcg
     1261 attcgatgcc cgatgttgga gtcaggacat ctggcgacaa agttggacca gacatcctta
     1321 gactgtgctg tatattccct gatcgtaagc attataatat atctatcggc agcccttatc
     1381 aaattcattc atgtgcaaat cgggggtgga atggcatgag atcagaactc taagagctcc
     1441 cgtacacgat cttctctttt atccatcgaa attgaagata taatctttgt ctcaggctga
     1501 gtttatttcc tcccttttct atttccctca ggtaatccag aatctcagag gcgggaacga
     1561 accagccctc ccgtgaggca aggtccttaa gcctctccac cgtggtatca tttatctctc
     1621 cattcctgca aaaatcgtag gcaaaatgtg tatagacaat gcagaccccg ccctcttcct
     1681 cgagcttatc cagattctcc tcgctcaaca agtcgttaaa tgcatgaacg tccatgccgt
     1741 cgctggtgtg aaaccagaag ttcacatagg gtctcttcgg atcatggtat ggaatatccg
     1801 gattgacttt tctgaggttg atttcataaa aactgaaatt gggtacatat tcgatatatt
     1861 ctttggcaat atctccccag aagaattctg tctcgggtag atgcccgaca taatcaacat
     1921 catccctggt cagattatac aacattctaa gaggccaata atcgagcctg tagacgcccc
     1981 aatatagatt ctccgcgtta tgataatgat tgatgtatat tctgggataa tatcctataa
     2041 gtgaatcgaa cacatgcagc gacctgatag tctcgtcacg tttcgagctt ccccctctgg
     2101 cgccatggga ggcaatttcg aatcctctat ctctcagacc caggacgaaa tccagatata
     2161 ctgaatcacc gagagtctga ccctggtccg gaaatgcttc acttgtagag gggaataccc
     2221 acacagtttt cgtggtcctg atgcccaggc tgtcaagcag tctataaatc gggctgacat
     2281 tttccagggt agatgcatca gtgtcgtcgc atatgctgaa tgcgaatttc tttccgtctg
     2341 ggaatgagat tctatcatat cccggggtca ggtataatga aaaagcgaaa gctaatacaa
     2401 ctatgacccc cagtactatt gccgatattt tgatcgcttt tctacacttc attttatgcc
     2461 agcctgcagg tctgttttag atacctgtaa ttctaattat tcactattga cattccacag
     2521 agaataggac
//
LOCUS       DSEB01000060            2460 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1006410, whole genome shotgun sequence.
ACCESSION   DSEB01000060 DSEB01000000
VERSION     DSEB01000060.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1006410"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1895
                     /locus_tag="ENO07_01155"
     CDS             <1..1895
                     /locus_tag="ENO07_01155"
                     /inference="COORDINATES: protein motif:HMM:PF07228.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00601.1"
                     /translation="ILLVTLFADTGKIVEFLQVESDVAFFGWVTDRFAFFVGFIAALL
                     DFLVRLHLQTFSAINIIYIIIALVLFYRGLRYISNKRLKTQTLIVIWGIQSAMGLYMI
                     AYLLPNLSGLHLSPNVQIGMAVAALAIGNGSVIYAIIRYQFLDVRLIVRQSVVYTITS
                     AIIVGAYLLIISQFTKILRDILGQETPILPAAFIILALIFFQPIMNQVDNFLKKFFIK
                     GKVDIRYVADEISREISSILDPEKLKTSIMRILREEMLIEKVRLCIKANDEAAYVLLP
                     QPNDPSPEMRFSAEDELTESLLRRNRPALIDEFRGSDRKSKTIESLEKMGYQMIVPLI
                     NRGKLDGFIALSNKISGYNYNYEDITTLNILSNQLVVAMSNAYLYQESLEKQRLEEEL
                     SLARQIQRDLLPKTVPVGDDFELAAYSEPSRYVGGDFYDFLQTRQNNTGIIIADVSGK
                     GMPAALMVSQIQAMLRSEVRNTDSIIRILNNVNFLMSTTTSSEKFVTLFYAEFDPVNK
                     IMQYSNAGHNYPIVVNQKGEHRFLIEGGLLMGAFKEATYKQDSLKLNPGDLVFFYTDG
                     VNEAFNHEGEQFGEERLLELIKDLRSLSAKEIADRVVKEVMDFSSSDMPQDDMTVVVL
                     KLKAPV"
     gene            1963..>2460
                     /locus_tag="ENO07_01160"
     CDS             1963..>2460
                     /locus_tag="ENO07_01160"
                     /inference="COORDINATES: protein motif:HMM:PF00293.26"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDS00602.1"
                     /translation="MAVYKHCPMCAAELVSFNDNGIKRSHCPRCGFIHYNNPAPAAGC
                     VVFQDGKLLWVQRAHEPKLGYWTIPAGFCEWEENPAETAVRELKEETNLDVKITGVFR
                     IYNGNDDPRTNAILILYFADVVGGELKASDDASRAEFLGLHESPENIAFESHKQALHD
                     LRTHYP"
BASE COUNT          633 a          570 c          627 g          630 t
ORIGIN      
        1 atatcctgct ggtaaccctg tttgccgaca ccggtaagat tgttgagttt cttcaggtgg
       61 aatcggatgt ggccttcttt ggctgggtca ccgacaggtt tgcgttcttt gtgggattta
      121 ttgcagcgct tctggacttc ctggtacgcc tgcatctgca gacattttcg gcgatcaata
      181 tcatctatat cataatcgca ctggtcctct tctatagggg cctgaggtat atatccaaca
      241 aacgcttaaa gacccagaca ctgatcgtga tctggggcat acagtcggcc atggggctgt
      301 atatgatcgc gtatctgctg cccaatttaa gcgggctgca tctctccccc aatgtgcaga
      361 tcggcatggc cgtggcagcc ctggcaatcg gtaacggctc ggtcatctat gccatcatca
      421 ggtaccagtt cctggatgtg cgcctgattg tacgtcaatc agtggtttat accattacct
      481 cggccataat cgtgggagca tacctgttaa taatatcgca gttcacaaaa atacttcggg
      541 acatcctggg ccaggagacc ccgattctgc cggccgcatt tatcatcctc gcgctgatat
      601 tcttccagcc gatcatgaac caggtggaca acttcctgaa aaaattcttt atcaaaggca
      661 aggtcgatat cagatatgtg gccgacgaaa tttcgcgtga aatctcgtcc attctagatc
      721 ccgaaaaact caaaacctcg attatgcgga ttctgcggga agagatgctg atagaaaagg
      781 tcaggctgtg cataaaggcc aatgatgaag cggcctatgt actcctgccg cagcccaatg
      841 atccctcgcc tgaaatgagg ttctccgccg aggacgagtt gaccgaaagt ctgctgagga
      901 ggaaccgccc tgccctgata gatgaatttc ggggctctga ccggaaatca aaaactatcg
      961 agagcctgga gaaaatgggc taccagatga tagtccccct tataaaccgc ggtaagcttg
     1021 atgggtttat cgcactttcc aataaaattt ccggttataa ttataactat gaggatataa
     1081 ccaccctcaa tattctatcc aaccagcttg ttgtggcgat gtccaatgcg tatctttatc
     1141 aggaatcgct cgaaaagcag aggctggagg aggaactctc cctggcccgg caaatccagc
     1201 gcgacctgct gcccaaaaca gttccggtgg gggatgattt cgagctggcg gcctactccg
     1261 agccatcccg atatgtcggg ggcgatttct atgattttct gcagaccagg cagaataata
     1321 ccggaatcat tattgccgat gtatccggca aagggatgcc tgcagcattg atggtttcgc
     1381 agattcaggc catgctgcgg agcgaggtgc gcaacaccga ttcgattata cgaattttga
     1441 ataatgtcaa cttcctgatg tccaccacca cctcatctga gaagttcgtg accctgtttt
     1501 atgcggagtt tgatccggtc aataagatta tgcaatactc caacgccgga cacaactatc
     1561 cgatagttgt taatcaaaaa ggagaacatc gctttttaat tgagggcggg ctgttgatgg
     1621 gtgcatttaa ggaggccaca tacaagcagg attctttgaa actgaatccg ggcgaccttg
     1681 tattcttcta tacagacggt gttaatgaag catttaacca tgagggagaa caattcggcg
     1741 aggaacgtct gctggaactg atcaaagatc tgcgctcctt atcggctaag gaaatagccg
     1801 acagggttgt caaggaggtg atggattttt cctcctccga tatgccccag gatgatatga
     1861 cagtggtggt cctgaaactt aaagcgccgg tttagagcga ttttcaaact tgccaatttg
     1921 tgcgggccgg tataagttgt atattaaatg aaaggtagat gaatggctgt atataagcat
     1981 tgtcccatgt gcgccgcgga actggtgagt ttcaacgaca acggcatcaa gaggagtcat
     2041 tgtcccaggt gcggttttat acattacaat aatcccgctc cggctgccgg atgtgtggtt
     2101 ttccaggatg gtaagctatt gtgggtacag cgcgctcatg aacccaaact cggctactgg
     2161 acaattccgg cgggtttctg cgaatgggag gaaaatccgg ccgagaccgc cgtgcgcgaa
     2221 ttgaaggaag agacaaatct ggatgtaaag ataacaggag ttttcagaat atataacggc
     2281 aatgatgatc ctcgcaccaa tgcaatttta atattgtatt ttgcggatgt cgtggggggc
     2341 gaattgaagg catccgatga tgccagccgg gccgaatttc tgggattgca tgaaagtccc
     2401 gagaatatcg cgttcgagtc ccacaaacag gcattgcatg atttaaggac acattatccg
//
LOCUS       DSEB01000061            3225 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003521, whole genome shotgun sequence.
ACCESSION   DSEB01000061 DSEB01000000
VERSION     DSEB01000061.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003521"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(206..562)
                     /locus_tag="ENO07_01165"
     CDS             complement(206..562)
                     /locus_tag="ENO07_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00603.1"
                     /translation="MTQTVHLTSSAQLYENEYGDLAIRFENNEVFEGVGTHSDKSFAA
                     EALEFIKNGARPAQWQEMPYRKLQHDGHGWRLVSSIGFLDGDETRPAVGLDIKPEELG
                     ARARRYLQPDMPQILT"
     gene            1134..1772
                     /locus_tag="ENO07_01170"
     CDS             1134..1772
                     /locus_tag="ENO07_01170"
                     /inference="COORDINATES: protein motif:HMM:PF00072.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDS00604.1"
                     /translation="MAVTLVAADPHPLSLLGLAQLLKSESELTLLDVCTTAAETVQAV
                     RKHQPDLLVIDINLPDRSGLELINELKNSSLEVKIIILTVAMDEGQTIDALRFGVKGV
                     VLKNMPSHLLVQCLQKVAAGGLWMEKESIGHAFEKMLQREAGMRRLVTILTAREIEVM
                     QCVASGLSNQQIADKLIVSEGTVKIHVHNIYRKLGINNRVDLTLYAQKRGIA"
     gene            complement(1986..2453)
                     /locus_tag="ENO07_01175"
     CDS             complement(1986..2453)
                     /locus_tag="ENO07_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HDS00605.1"
                     /translation="MMGLIDVQKAIKDSIQTEKDAMDYYKYGAERMAEDKARQTFEIL
                     AREEYQHAESFYRIYPGDDITSFEDFMNAPPNTDSSWWKNLQSLMVQDFDERKALELA
                     IEQEDALEKSLREMAAKMEDPDIARVYLANAVSTHNHLELVEEDYRAMLGMSG"
     gene            complement(2717..3073)
                     /locus_tag="ENO07_01180"
     CDS             complement(2717..3073)
                     /locus_tag="ENO07_01180"
                     /inference="COORDINATES: protein motif:HMM:PF07238.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HDS00606.1"
                     /translation="MHNRRFRRIPFEAEVTLNVGHDTWPGELLDVAMKGAMVGTDKPL
                     PLALGANCSLCISLPGTPISLDFQVVLIHCEGHHYGFRFISENLETFTHLRKLIELNT
                     GDAEATRSELSAWICE"
BASE COUNT          778 a          771 c          803 g          873 t
ORIGIN      
        1 aatatgtgcg gtgggccacc aagcatgttg agcgatatcc tggcggccct gcaccgtctt
       61 gattcagaga ttgacactga gcattcccct cctactaaac agggggggga acagtagaag
      121 gtagcaagta cggcatcgac gcctatctgg aaatcaaata tttgtgcatt ggtgagctcg
      181 gctgaagcga tttccaggcc acgaattatg tgagaatttg cggcatatcc ggttgcaggt
      241 aacgtctggc gcgcgctccc aactcctcag gtttgatatc caggccaacg gccggtctcg
      301 tttcgtctcc atcgagaaat ccgatactgc tcaccagacg ccagccatga ccatcatgct
      361 gaagtttgcg gtatggcatt tcctgccatt gcgcaggacg cgcgccgttt tttataaact
      421 caagggcttc ggcagcgaag ctcttatcag aatgcgtgcc aacaccttca aagacttcgt
      481 tattctcaaa cctgatggcc aggtcgccgt attcattttc ataaagctgc gcgctcgaag
      541 ttaaatgcac ggtctgagtc atctcattct ccttctggtt gtctgtatgc aattccggtt
      601 atctctatac agtttactgc aggtctttgt tcccgcaaac ccggcgttgc ctgcatctcc
      661 gattgatctg gaccgtgaaa tatccggatt tagttttcat ggggaactcc ggacaacgca
      721 agcacagttt ttgtaggctt ccgggaaggg ctcccgcggc tgtcaccttg attctcattg
      781 tatgtgtgat aggtatttat taaagatggg ttgaactgca ggtcacagag gagtagccaa
      841 tgcggcaaat acgaaaagtc aaagatttga ccggttacca tctaaaagcc catgatggcg
      901 aaatctgcgg ccgattaagc tttttatttt ttttgcgcct tgagttctct cctacagatg
      961 tctactggag ttttgtagat aaccaagaaa ctttattgtg gttggcacag ggccttgata
     1021 cttgatacta ttcctcagag tcgaagggac ttttggcaga ttccgcgatc aagtttttaa
     1081 aatcgcgata tatccggcca ccaaattgca attttattga atccggagat taaatggctg
     1141 tcacgttagt tgctgcagat ccccatccgc tctctttact cgggctggct caactgctta
     1201 aatccgagtc agaactgact ctccttgacg tttgcacgac cgctgctgaa acggtgcaag
     1261 cggtacggaa acatcagccg gacctgctgg ttattgatat taatctgcct gatcggagtg
     1321 gcttggagct gatcaatgag ctgaaaaata gctcgctgga ggttaagatc attatcttga
     1381 cagtcgccat ggatgaagga cagacgattg atgccctgcg ttttggtgtc aagggcgtag
     1441 tactgaagaa tatgccctct cacttactgg tgcagtgtct gcaaaaggtg gcggccggtg
     1501 gtttgtggat ggagaaagaa tcgatcggac atgcgtttga aaagatgctg cagcgagaag
     1561 ccgggatgcg gcgacttgtc acaatcctca cggcccggga aattgaggtg atgcagtgcg
     1621 tggccagcgg cttgagcaat cagcagattg ccgataagct gatcgtgagt gaagggacgg
     1681 tcaagataca tgtccataat atctatcgta agcttggaat caataaccgg gttgatttga
     1741 cgttatatgc gcaaaagcgt ggcatagcct gattttcgtc tggaaatggt ccttttccgc
     1801 aatctctggt acgtaatccg cagatttgtt tgaccttgcg aaaatttgca catttccgga
     1861 tcaaacaccg cgaaaactgc gtttatgcga tccagggttg attgacagct ggccatgatc
     1921 aaaacgaaaa ggccgcccca tttcgaggcg gccttcagtt tttaaaaaca tttcagcggt
     1981 aagaatcagc cactcatgcc gagcatggcc ctgtagtctt cttctaccag ttccaggtgg
     2041 ttatgagtcg agaccgcatt cgccaggtag acccttgcga tatcggggtc ctccatcttt
     2101 gcagccatct cacgcaggct tttttccagc gcatcttcct gctcaattgc gagctccaat
     2161 gctttacgct cgtcaaaatc ctgtaccatc aggctttgaa ggttcttcca ccaggaagaa
     2221 tccgtgttcg gtggcgcatt catgaaatcc tcaaaagaag tgatgtcatc tccgggatag
     2281 attctgtaaa aggattcggc gtgttgatat tcttcccgtg cgagaatctc gaaagtctgg
     2341 cgggctttgt cttccgccat ccgctctgca ccatatttgt agtaatccat ggcgtctttt
     2401 tcggtctgga tggagtcctt gatcgccttt tgcacgtcaa tgagtcccat aatattaaac
     2461 ctcccttgtt ctcaatttat acgtaagatt tagccggcac atttataaca ttttttaagg
     2521 cacaggtctt gtctttttct tccatgtgga cagtggtttt aagacacatt gcaaacatga
     2581 gacccctgat tctatggaga tgtcagggac atgccgaaat ggagatttac gttctgagcc
     2641 tctcccattg tatggcgcag aatacgtctt aagccggcca gcaatccaac gtcatgaacc
     2701 cgccggtctg gattgatcac tcgcaaatcc aggcggataa ttcgctgcgg gtcgcttctg
     2761 catcacctgt gttgagttcg atcagtttac gcaagtgggt aaaggtttcc aggttttcgc
     2821 tgatgaatct gaaaccgtaa tggtggcctt cgcaatggat cagcacaacc tgaaagtcga
     2881 gagagatagg cgtccccggc aaggaaatgc agaggctgca attggcccca agggcgagag
     2941 gcagtggttt atcggtgcca accatggccc ctttcatcgc aacatcaagg agttcgcctg
     3001 gccaggtatc atggccgaca tttagcgtca cttcagcttc aaaaggaatt ctacggaatc
     3061 ggcgattgtg catcggctct ttctttcctc aattagtttg ttggaattct aacgcagaaa
     3121 cagtgtgacg caagcgatcg catcaagacg agcctggctc tgtcggctcc ggtgagccga
     3181 aatcaagacc gcacaattca aaaaaaccat gtatcaatcg atagg
//
LOCUS       DSEB01000062            2603 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1005645, whole genome shotgun sequence.
ACCESSION   DSEB01000062 DSEB01000000
VERSION     DSEB01000062.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1005645"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            30..1232
                     /locus_tag="ENO07_01185"
     CDS             30..1232
                     /locus_tag="ENO07_01185"
                     /inference="COORDINATES: protein motif:HMM:PF00079.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HDS00607.1"
                     /translation="MRGVLTLIVAVILVLSPILAGQDQAKVSFTNDELEQIEAINCFG
                     FKFFHTFCELDTADNIVISPFSMHYALGMTNNLAFSDTKDSIDEVLCVSGTNVRLVNN
                     RYFGLMEKMPALDSNVILEIANSIWVRGGIAKEYSDLFTRRFNADVNTRDFDDPKTRE
                     EINDWVSKKTHGKIKFALEKPIPGGAVMYLMNTVYLEALWSSQFSIENNLTGKFFVTN
                     EESLQWETMSQKSSYSYFINEDLRGIEIPYGKGVFSLLVFVPPNPSDIDELAKSFIQD
                     SLESWTSRMETDSVTLFLPKIKVIYGGDIGGILANMGMSKAFNGGTACFPGALYIGQA
                     LHKTSFQMDEEGTIAAAFSSIRLDSIREPNSGAKFYVDRPFLLVIRDNYTHGILFIGK
                     IKRPVWED"
     gene            complement(1448..2458)
                     /locus_tag="ENO07_01190"
     CDS             complement(1448..2458)
                     /locus_tag="ENO07_01190"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDS00608.1"
                     /translation="MTNKISHDSQELLDPVMRLLRDNPEKTYSIRDIAKKIDIPDKTA
                     REVIGGLSAWGYRFELDDRSRFRFLSAPDAIFPFEIQYGLKTKYLGSEISSHYTVTST
                     NTIAHQMAESGAPEGTLVIAERQRGGKGRLGRVWHSPPKLGLWFSLILRPDIDPAAAP
                     SLSILSALTLSEILRSKYKLQSFIKWPNDCLIDNHKVTGILTELSAENHKIDFVIVGM
                     GINVNLAAKDFPPFLRSTATSLYLETGDKIRRVDLLRRYLEAFEKNYDIFKTKGLKPF
                     LPRIKRHSLLLGKKIKLKIGRKIIQAEAHDIDSDGALLARHRKEILRVTAGEVTVIEH
                     SK"
     gene            complement(2455..>2603)
                     /locus_tag="ENO07_01195"
     CDS             complement(2455..>2603)
                     /locus_tag="ENO07_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HDS00609.1"
                     /translation="SDVEIEASGNVSLGSVRRIANTGVDYISIGKLTHSAPALDLSME
                     IVPL"
BASE COUNT          696 a          612 c          641 g          654 t
ORIGIN      
        1 acttaaaata aaaacattgg aggtgtaaaa tgcgaggggt actgacatta attgttgccg
       61 ttatcttagt tctgagtccg atattggcag gacaggatca ggcaaaggtg agttttacca
      121 atgatgaatt ggagcaaatc gaagcaatca actgcttcgg cttcaagttc ttccacacat
      181 tctgcgagct cgacaccgcc gataatatcg tgatctcgcc attcagcatg cattatgccc
      241 tggggatgac caataatttg gcgttttctg acacaaaaga ctcaatagat gaggttcttt
      301 gcgtatctgg gacaaatgta cgtcttgtta ataacagata ctttggtttg atggaaaaga
      361 tgcctgctct ggattctaat gtaattctcg aaattgcaaa ctcgatctgg gtaagagggg
      421 gtattgcaaa agaatacagt gacctgttca caaggagatt caacgcagac gtgaacacta
      481 gggattttga cgaccccaaa accagggagg aaataaacga ttgggtttca aaaaaaacgc
      541 acggcaaaat caaattcgca ttggaaaaac ccatcccggg gggtgcagta atgtacctaa
      601 tgaatactgt atatctggag gcattatggt cgtcacaatt ttcgatagaa aataatttga
      661 cgggtaaatt ctttgtcaca aacgaagaaa gcctgcaatg ggaaacaatg agccaaaaga
      721 gtagctattc gtattttatc aatgaagact tgcgtggaat cgaaatcccc tatggaaaag
      781 gagtattcag tctgctggtc tttgttccgc ccaatcccag tgacattgac gaactcgcaa
      841 aatcattcat ccaggacagc ctagagagct ggacatcacg aatggagact gactctgtga
      901 ccctattcct cccgaaaatc aaggtaatat atggcggaga cattggcggt attcttgcaa
      961 atatggggat gagtaaggca ttcaatggcg gcacagcatg tttcccgggt gcgttatata
     1021 tcggtcaggc attacacaag acctctttcc agatggatga ggagggcaca atcgcggcag
     1081 cgttttcctc tattcgcttg gattccataa gggaacccaa cagcggtgcc aagttctatg
     1141 tggacagacc gtttctcctt gtcatcagag acaattatac acacggcatt ctattcattg
     1201 gcaaaataaa aagacctgtc tgggaagatt gataagtgta tcagtgaata atccccgttc
     1261 cgtcaggtcc tcagacctga cggacggcag atctgaggat ctgcctcaac gcggaattag
     1321 atttccgcat gggtgcccgc cacaaattca ttttctaacc gatggttgat tcaaggattc
     1381 gaaaaaaata tcccatccct tatgggccga gatcggccca ccggggatgg gggcacccaa
     1441 aatcacatta tttactgtgc tcgatcacag tgacctcgcc ggcggtgacc cgcaggattt
     1501 ccttgcggtg cctggccaga agcgcgccgt cggaatcaat atcatgcgcc tccgcctgaa
     1561 taatcttgcg cccgatcttg agcttgatct tcttgcccag taagagcgaa tgtcttttga
     1621 tgcgcggaag gaacggcttc aggcccttcg ttttgaaaat atcataattt ttctcgaaag
     1681 cctcgagata tctccgcagg agatccaccc gcctgatctt atcgccggtt tcgagataga
     1741 gcgaggttgc ggttgacctt aaaaagggcg gaaaatcttt tgccgcaagg ttgacattga
     1801 tccccatccc cactatcaca aaatcgatct tatgattctc ggccgaaagt tcggtcagga
     1861 ttccggtcac cttgtggttg tcgatcaggc agtcattggg ccatttgatg aatgattgca
     1921 gtttatattt cgagcgcaat atctcactca gggtcagcgc ggacaaaatc gacaggctgg
     1981 gagctgctgc cggatcgata tcgggacgca gtatcagcga aaaccataac ccgagtttgg
     2041 gcggcgaatg ccatacccgt cccagacgtc ccttgccccc gcgctgccgt tcggcaatca
     2101 ccagcgtccc ctcaggcgca ccgctttcag ccatctgatg cgcaatggta ttggtggaag
     2161 tcactgtata atgggacgat atttcgctgc cgagatattt ggttttcaag ccgtattgga
     2221 tctcaaaggg aaagatggca tccggggcag agagaaatct gaaccgcgag cggtcatcga
     2281 gttcgaaccg atatccccat gccgaaagcc cgccaatcac ctcacgggca gtcttatccg
     2341 ggatgtcgat cttctttgcg atatccctga tcgaataggt cttctccgga ttgtcgcgca
     2401 ggagacgcat aaccgggtcg agcaactcct gtgagtcatg tgatattttg tttgtcatag
     2461 cggtactatt tccatgctga gatcgagagc gggcgccgaa tgagtgagct tcccgatcga
     2521 aatataatca acgccagtgt tggcgattcg tcgcacgctt ccaagactga cattgccgga
     2581 ggcctcgatt tccacatcag aat
//
LOCUS       DSEB01000063            1797 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1012809, whole genome shotgun sequence.
ACCESSION   DSEB01000063 DSEB01000000
VERSION     DSEB01000063.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1012809"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            500..1024
                     /locus_tag="ENO07_01200"
     CDS             500..1024
                     /locus_tag="ENO07_01200"
                     /inference="COORDINATES: protein motif:HMM:PF07603.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HDS00610.1"
                     /translation="MMHRCGLVICCFVLFLCGSVSVVNGQEESKDDGFVKLDIQGREL
                     PPDAEQWAMVRDRATGLVWEVKTTDGSIHDRERTFNWEDAQETFLAELNSKSFGGFSD
                     WRLPTTDELRTIMVKGAEPYINQDFFPNTVSTSYLSWRKCGSGEIFDERVKFGKIRNS
                     KKDRRVRAVRSGLE"
     gene            complement(1029..1442)
                     /locus_tag="ENO07_01205"
     CDS             complement(1029..1442)
                     /locus_tag="ENO07_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00611.1"
                     /translation="MQTDEYRVVAHYLGGRLAKGYTFDFNQNRETFRLFAGPEQNEEG
                     TLVYHEELKALFFVKTFEGNPRYNDPIFSEEDIKRLVGMKLKVIFKDGEVMYATTFGY
                     SPARKGFFVYPIDKQCNNEKVYVVTSSTTTIEIIR"
BASE COUNT          480 a          379 c          470 g          468 t
ORIGIN      
        1 aagcggtttt cattacgagt accacaacgt gattaattaa gtaccacaga aggggggcat
       61 ttaaaacgtg gcatattgtc gcacaatgcg gaaatattga cgattatagg ttataagaaa
      121 aaagccattc atgaatatgg atattttatc catgcaatga ggaattcttc atggaagtcg
      181 gcaataatgt ttgtatttgt ttgatttttc atttcacagg aagggggcgg ccttgatttg
      241 cttaaggctt tccatgtgtg ataaaataca tttcaataaa taattcaggg tggttttttc
      301 ctgttcgctg aagggagctt tacagcttat tgttcataat ttgcgggaag ctcgccgaaa
      361 gagcgtgcgg cactgatcaa aacgctcatg gtaaacgcca aacgggttag ccggctgcca
      421 gaacgggtta gccgatcaag ataaacgtaa gtcagggatg attaatgaat tcagctgatt
      481 tcaacaagag gtaagattga tgatgcatcg ttgcggactg gttatttgct gttttgtgct
      541 ctttctgtgt ggaagcgtat cggtggtaaa cgggcaggag gagtcaaaag atgatggatt
      601 tgtcaagctt gatatccagg ggcgggaact gcctcctgat gcagagcagt gggcaatggt
      661 cagggacagg gcgaccgggc tggtctggga ggtgaaaacg accgacggct caattcatga
      721 ccgggaaaga acgtttaact gggaggatgc ccaggagaca tttctggcgg agttgaactc
      781 caagagcttt ggcggttttt ccgactggcg cctacccaca acggacgagc ttcgaaccat
      841 catggtcaaa ggggctgagc catatatcaa ccaggacttt tttcccaaca cggtatcgac
      901 cagttatctt tcctggcgaa aatgcggcag cggggagata tttgatgagc gggtcaaatt
      961 cggcaagatc cgtaattcca aaaaagatcg acgggtccgg gctgtccggt cgggactgga
     1021 gtaggtcgct acctgatgat ctctatggtc gtggttgagg aggtcaccac gtagaccttt
     1081 tcgttattgc actgcttgtc aataggatac acaaaaaagc ctttacgggc cgggctgtag
     1141 ccaaaggttg tcgcatacat cacttcgcca tccttgaaaa tgactttcaa tttcatgccg
     1201 accagccgtt tgatatcctc ttccgaaaag atcgggtcgt tatacctcgg gtttccttca
     1261 aaggtcttga caaaaaagag ggccttcagt tcttcatgat agaccagggt tccttcttcg
     1321 ttctgctccg ggccggcaaa gagacgaaat gtctcacggt tctggttgaa gtcaaaggta
     1381 tagcccttgg cgagccttcc acctagataa tgggcaacca ccctgtattc atctgtctgc
     1441 ataacaggcg cctttggctg gaaaaaatgc ggtcatcgta caccatgagg tgctgacaat
     1501 gttccccgac tgatcggatc atcttttata cgggagtata cctgtattgc cataaaataa
     1561 caaattccgg gtaccgacaa tgtcaaattt cattaattgc agctgttcaa ccggttgcgg
     1621 ccgataacca gcaagggctt caaggcgccg ctgtttctct gaaaaaatta tggttcatcg
     1681 ccgcaatgga gagagaagca agccttgtcg tataataatt ggcgtcccca accggatttg
     1741 aatcccgaca atgtcgggac ctgaaaggca gtatttgcga acaggacatg aaaaagg
//
LOCUS       DSEB01000064            3445 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003037, whole genome shotgun sequence.
ACCESSION   DSEB01000064 DSEB01000000
VERSION     DSEB01000064.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003037"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..971
                     /locus_tag="ENO07_01210"
     CDS             <1..971
                     /locus_tag="ENO07_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDS00612.1"
                     /translation="FDANDGVILIAATNRPDVLDPALLRPGRFDRRIVVDQPDVKGRR
                     GIFEVHVRKINLADDVDLEILARATPGMSGADIANMVNEAALLAARREHDKVYMVDME
                     DAKDKVILGTERRSLVINEEEKKTTAYHEAGHALLGKLLPKADPVYKVTIIPRGMSLG
                     QTSSLPVDEKRTYTKEYLEAMLVVFMGGRVAEKLVFDHLSTGAGNDLARATELTRKMV
                     CDWGMSESLGALTFGKKDNEVFLGRDIIRDRNYSEETARQIDIEVRKIIEDAENRARE
                     LLEANVDKLHKIAEALLERELLDGEDLEKIINGEELEEIPAPSSKT"
     gene            977..1825
                     /gene="folP"
                     /locus_tag="ENO07_01215"
     CDS             977..1825
                     /gene="folP"
                     /locus_tag="ENO07_01215"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDS00613.1"
                     /translation="MRSELRPIIIGSSRFDFSRPYVMGILNVTPDSFSDGGNFFAKDK
                     AVEHALRMQDEGADIIDIGGESTRPGSDPIGVDEELERVLPVIEALAPEIKVPISIDT
                     RKARVAEIALESGASLINDITALRGDNQMAALAASRNVPVIIMHIKGTPKNMQKNPVY
                     VDLIGEISEYFEESIGIAEAAGLKRDKLILDPGIGFGKTYENNFELISRLGDFKKFGL
                     PLIVGASRKRFLSIDNKYPETERLEQSLAAACAAVANGADFVRVHDVLSTKKALWAFS
                     NIDISR"
     gene            1901..2665
                     /locus_tag="ENO07_01220"
     CDS             1901..2665
                     /locus_tag="ENO07_01220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDS00614.1"
                     /translation="MEIFSYQFLDFTVIDLLDVLVVAFIIYKLLELVKGTRSAQMMIG
                     LVFIFMVAFLAYWFQLEGLKWLFTNLATVGFIVLVVVFQPEIRGGLAQIGHTRFMRRF
                     VKSESEHAVDEVVKGAIRLSELRYGGLIVLERQVGLKNIIATGREINAEVSSDLLTTI
                     FTPYTPLHDGAVILRNEKLLAAACVLPMTQNPRYANLFGMRHKAAIGVTEESDAVCLV
                     VSEETGAISITHQGKFKRDLEKLTLRESLMEILEMS"
     gene            2668..>3445
                     /gene="obgE"
                     /locus_tag="ENO07_01225"
     CDS             2668..>3445
                     /gene="obgE"
                     /locus_tag="ENO07_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDS00615.1"
                     /translation="MFVDLVEIEVQSGKGGAGCVSFRREKYVPKGGPDGGDGGKGGSV
                     YIQVDSNLNTLMDLKYKRKYKAENGEPGKGGNKSGEDGKDVTVKVPPGTMVFNAENDQ
                     LLADLVEDQDRYLAARGGIGGRGNTHFKSSTNQSPRRADPGRPGESFRLRLELRLIAD
                     VGLVGYPNAGKSSLLKAMSEAKPKIADYPFTTLNPNIGIVKIAEFETLKMADIPGIID
                     GASRGKGLGLQFLRHIERTSVLLYILDGGKNDFMEDYKVLQ"
BASE COUNT          892 a          800 c          943 g          810 t
ORIGIN      
        1 gctttgatgc caatgacggc gtaatcctga tagccgccac gaaccgtccc gatgttcttg
       61 acccggccct tctgcgtccc ggacgatttg accgacggat tgtggtcgat cagcccgacg
      121 tcaaggggag acgagggatt ttcgaggtgc atgtccgaaa aataaatctg gctgatgatg
      181 ttgatcttga aatcctggcc cgtgccaccc ccggcatgtc gggagccgac atcgctaaca
      241 tggtaaatga ggcggcgctt ctggctgccc gccgggagca tgacaaggtc tatatggtgg
      301 atatggaaga tgccaaggat aaggtcattc ttggaaccga aaggcgctca ctggtaatca
      361 atgaagagga gaaaaagacc accgcatatc atgaggccgg gcatgcgctt ttggggaagc
      421 ttctgcccaa ggctgatccc gtttataaag tcactattat tccccgcggc atgtctctgg
      481 gacagacttc ctcgcttccg gttgatgaga agcgtaccta caccaaggaa tatctggagg
      541 caatgctggt agtattcatg ggcgggcggg tggccgaaaa gctggtcttt gaccatctct
      601 ccaccggcgc cggcaacgat cttgcccggg caacagagct gacacggaaa atggtctgcg
      661 actggggcat gtccgaatca ctcggcgctc ttactttcgg caagaaagac aacgaagtct
      721 tcctgggcag ggacatcatc cgggatcgca actactccga ggaaactgcc cgtcagatcg
      781 atatcgaggt acgtaaaatt atagaagatg ccgaaaaccg ggccagggaa cttctggaag
      841 ccaatgttga taaactgcat aaaatcgctg aagctcttct ggaaagagaa ctgcttgacg
      901 gcgaggacct ggaaaaaatc ataaatggtg aagaactgga ggaaattccg gccccctcct
      961 ccaaaacata gaattgatga gatcagaatt aaggccgatt attatcggca gcagccgttt
     1021 tgatttttcc cgtccctatg ttatgggaat cctgaatgtt actccggatt ccttctcaga
     1081 tggcgggaat ttttttgcaa aagataaggc cgtcgagcat gcattgcgca tgcaggatga
     1141 gggcgcggat attatagaca tcggcggcga atccacacgc cccggttctg atccaattgg
     1201 cgtagatgaa gaactggaac gggtgctgcc ggtaatcgaa gcgctcgctc cggaaataaa
     1261 ggttcctatt tctattgata cccgcaaagc gcgggtagct gaaatcgcgc tcgaatcggg
     1321 cgcttctctg atcaatgaca ttaccgccct gagaggtgat aatcaaatgg cggcgctggc
     1381 ggcctcgcgt aatgtgccgg taataatcat gcatattaaa ggcaccccta aaaacatgca
     1441 gaaaaatccc gtctatgtgg atttgattgg cgagatcagc gaatattttg aggagagtat
     1501 tggtatcgct gaggcagctg gcctaaagag agacaaattg attctcgatc cgggcatcgg
     1561 attcggcaag acctacgaaa ataattttga gttgataagc aggctcggag atttcaaaaa
     1621 gttcggcttg cctcttattg tgggagcctc gcgcaagcgc tttctctcca tcgataataa
     1681 gtacccagag acagagcgtc tggagcaatc gcttgcggcg gcctgcgcgg cggtggcaaa
     1741 cggcgctgat tttgtgcggg tccatgatgt gctttcgact aaaaaagcgc tctgggcctt
     1801 ttcaaatatc gacatttctc gataaattct atcttgcttt caagtttact tgcaatttta
     1861 aaaaaataat aatatcatgt tgttttagcc tggaaataag atggaaatat tcagctacca
     1921 atttctggat tttacggtaa tcgaccttct cgatgtcctg gtcgtggcat tcatcatcta
     1981 caagctcctg gagcttgtca aaggcacccg ttcggcccag atgatgatcg ggctggtctt
     2041 tattttcatg gttgcttttc tggcttattg gttccagctg gagggattga agtggctctt
     2101 taccaatctc gccacagttg gtttcattgt gctggtagtg gtctttcagc cggaaatccg
     2161 cggcggcctg gcccagatcg gacatacccg cttcatgcgg cgctttgtca aaagtgaatc
     2221 ggaacatgcc gtcgatgagg tggtcaaggg cgccatcagg ctttcggaac tgcgttacgg
     2281 cggacttata gttctggagc ggcaggtcgg gctgaaaaat attatcgcta ccggaaggga
     2341 gatcaatgcc gaggtctcct ctgaccttct aacgacaata tttacaccat atacaccact
     2401 gcacgatggc gcggtaattc tcaggaacga gaagttactg gctgccgcat gcgtgcttcc
     2461 aatgacccaa aatccccgct acgcaaatct ctttggtatg agacacaagg ctgctatcgg
     2521 cgtaaccgag gaatcggatg cggtctgcct ggttgtctcg gaggaaaccg gagcaatttc
     2581 aatcactcat cagggaaaat tcaagcgtga tttggagaaa ctaactctga gggaaagctt
     2641 gatggaaatt ctcgaaatgt cctgaaaatg tttgtcgatc ttgtagaaat cgaagtccag
     2701 agcggaaagg gcggggccgg atgtgtatcc tttcggcggg agaaatatgt tcccaagggc
     2761 ggtcccgatg ggggcgacgg cggtaaggga ggatctgtat atatacaggt ggacagcaat
     2821 ctcaataccc tgatggactt gaaatacaag cgcaagtaca aagcagaaaa cggcgaaccg
     2881 ggcaaaggcg gcaataagag cggagaggac ggcaaagatg ttactgtgaa ggtgcccccg
     2941 ggaacgatgg tcttcaatgc cgagaatgat cagttgctgg ctgatctggt tgaagaccag
     3001 gacaggtacc tggcggccag aggaggaatc ggcggacggg gcaataccca tttcaagtca
     3061 tccaccaacc agtcaccgcg gcgcgccgat ccgggaaggc ctggggagag tttcagactg
     3121 agattagagc ttagactcat tgccgatgtc ggcctggtgg gttatcccaa tgccggcaaa
     3181 tcaagtctct tgaaagcgat gtcggaggcc aaacccaaaa ttgccgacta tccattcacc
     3241 accctcaatc ccaatatcgg catagtcaaa attgcagaat ttgaaactct caaaatggcg
     3301 gatattcccg gaataataga tggagcgagt cgggggaagg gcctgggtct tcagttcctg
     3361 cgccatatcg aaagaacctc tgtcctgctc tatattctgg atggcgggaa aaatgatttt
     3421 atggaagatt ataaagtgtt acaac
//
LOCUS       DSEB01000065            2813 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004747, whole genome shotgun sequence.
ACCESSION   DSEB01000065 DSEB01000000
VERSION     DSEB01000065.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004747"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..662)
                     /locus_tag="ENO07_01230"
     CDS             complement(<1..662)
                     /locus_tag="ENO07_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00616.1"
                     /translation="MKISNIRLVSIVIVTLIFGVSCSEGPREISLEQLQQRSDGLYYA
                     VNEDKPFSGVAVETYEDGQKSAEKSFEDGKLHGFIRAWYPDGNKKSEIGYDDGLQSGP
                     FRTWYDNGQQEMEGDYKDGRRTGEWSFWNRDGQKLEAGKVTDIDGNTYLTIKIGDQWW
                     MAENLKVTHYRNGEPIVHVTADSAWRRLSSGAYCSYGNDEDRADVYGRLYNYYAVADG
                     RG"
     gene            complement(848..1534)
                     /locus_tag="ENO07_01235"
     CDS             complement(848..1534)
                     /locus_tag="ENO07_01235"
                     /inference="COORDINATES: protein motif:HMM:PF00753.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDS00617.1"
                     /translation="MSLQIFPVKVGINTCYLIRHEGVILIDAGGPNKIKTFQRAFDKF
                     SIRPDEIKLIVLTHGDVDHVGSAADLKELTGARIAIHKNDKDLFEKSLHNFPAGVTKW
                     GKFMHVILNPVLKNVLPRISGGKADIILDENDYPLSDFGVQGKIIHTPGHTSGSISVL
                     LDSGDAFVGCMAHNNFPFRLKPGLPIFADDIEGVKQSWKRLLKYDIKTIYPGHGNPFP
                     AEVMRRELGE"
     gene            complement(1649..2590)
                     /locus_tag="ENO07_01240"
     CDS             complement(1649..2590)
                     /locus_tag="ENO07_01240"
                     /inference="COORDINATES: protein motif:HMM:PF16169.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HDS00618.1"
                     /translation="MPCMNYRITDFKHIPGKNCVTTALRNILNYYHILLSEELILGLA
                     GGLGFYYIELPGFPNPFIGGNSSNLIGRFCHNMDLQLFELRKENQEAAHKAMVGKILS
                     DIPVIVKIDLYYLDYFRSKFHFSGHRVIPVGIDDAYVYVADTGFRTIKQTSIENFKKG
                     RASGYGPRPPSNLQLFINIPEKNLPILDNLWGIIRRNARQMVSSPDDNGLVSLEKFAA
                     NADKFKDPEYLLIQIEKAGTGGALGRLMYRDFLSEANLYRPHQTLLQAYKLYAEVVEI
                     YEGIVKELKQETMPDLKKPLMEVLDYEKRAAELLAGL"
BASE COUNT          744 a          714 c          589 g          766 t
ORIGIN      
        1 ataccacgac cgtcggccac agcataatag ttgtacaatc ggccgtaaac atcagcacgg
       61 tcttcatcgt taccatagct gcaataagct ccactcgaaa gacgtcgcca cgcgctatcg
      121 gcggttacat gtacaatggg ttcgccgttg cggtaatgtg ttactttcag gttttccgcc
      181 atccaccact gatcaccgat cttgattgtg aggtaggtat tgccgtctat atctgtgact
      241 ttacccgcct cgagcttctg cccatcccga ttccagaagc tccattcgcc agttcgtctt
      301 ccatccttgt agtcaccctc catttcctgc tgcccgttat cataccatgt tcgaaacggg
      361 ccggattgca ggccatcatc gtagccgatc tcagatttct tatttccgtc ggggtaccat
      421 gccctgatga acccgtgcag tttaccatcc tcgaacgact tctccgcgct cttctggcca
      481 tcctcatagg tttcaaccgc cacaccgcta aagggcttat cctcgttcac agcataataa
      541 agtccgtcag accgctgttg cagctgctcc aatgagattt ctctgggacc ctcagaacag
      601 gagactccga aaatcaaggt cacgataaca atgctgacca agcgaatatt tgatatcttc
      661 atgttgccag tcctcctctc gataaattac gcatacactt cgtacattca cgtactcgac
      721 gaatataatc aaattgcgct ccacgtgaca ggtggtttcg cccgttattt ctactattta
      781 catattagcc tcattaaata gatcagagca ttcctacagc ttgggctgta ggggcacccg
      841 gactatttca ttctcccagc tcccgcctca tcacctccgc cggaaatgga ttgccatgac
      901 ccggatagat cgtttttata tcatacttga ggagccgttt ccagctttgt ttgacccctt
      961 cgatatcatc ggcaaatatc ggcaaacccg gcttcaatcg aaatggaaag ttgttatgcg
     1021 ccatacatcc tacaaaagca tcgccggaat ccagaagcac gctgattgaa cctgacgtat
     1081 gcccgggagt atggattatc tttccttgaa ccccaaaatc gcttagggga tagtcgttct
     1141 catccaaaat aatgtcagcc ttcccgccgg aaatacgggg taatacattt ttcaaaaccg
     1201 gattgagaat aacatgcata aattttcccc atttcgtaac tccggcaggg aagttatgca
     1261 gagatttttc aaacaggtcc ttatcatttt tgtgtatggc aattctggca ccggtcagct
     1321 cctttaaatc agccgcagac ccgacatgat caacatcccc atgcgtgagt acaatcaatt
     1381 taatctcatc ggggcgaatc gagaatttat caaatgccct ctgaaaggtc tttattttat
     1441 tcgggccgcc ggcatcaatt aaaataacac cctcgtgcct gatcagataa caggtattta
     1501 taccgacttt tacaggaaaa atctgaagag acatacgatc cccgatccaa aataatcacc
     1561 gctgtattta attaactcca ccggaggcaa taccctatcg tctcaaatat aactcagcgc
     1621 aaaatcaatt tcaacctgaa aacaacatct acaacccggc taatagctcg gccgctctct
     1681 tttcatagtc caacacctcc ataagcggct ttttcaaatc cggcatcgtc tcctgcttga
     1741 gctccttcac aatgccctca tagatttcga ccacctcggc atagagctta taagcctgca
     1801 atagagtctg atgcggacgg tacaggttgg cctcactcaa aaaatcgcgg tacataaggc
     1861 gtcccagcgc accaccggtg cctgcttttt cgatctggat cagaaggtat tctggatctt
     1921 tgaatttatc tgcattggct gcgaactttt ccagtgaaac taggccgttg tcatcgggag
     1981 aacttaccat ctgcctggca tttcgtctta taattcccca gagattatcc aggatgggca
     2041 gatttttctc gggtatgttt ataaaaagct gaagattgct cggcggcctg ggtccataac
     2101 ctgaggcacg tccctttttg aagttctcga tcgatgtctg cttgatggtt ctaaatcctg
     2161 tgtcggcgac atacacatac gcatcatcaa tcccgaccgg aataacacga tgaccggaaa
     2221 aatgaaattt gctccggaag tagtcgagat aatacaaatc tattttgaca atcaccggta
     2281 tatcggagag gattttgccg accatggctt tatgggcggc ctcctgattt tctttgcgca
     2341 gttcaaaaag ctgcagatcc atattatggc agaaacgtcc aatcaggttg gaggaattgc
     2401 cgccgatgaa tggattagga aatccgggca gctcgatata atagaaaccc aggccgccgg
     2461 ccaggccgag aatcaattcc tcggagagaa ggatatgata gtagttgaga atattcctga
     2521 gcgcggtggt gacacagttt ttgccgggga tatgtttaaa atcggtaatt cgataattca
     2581 tacagggcat tctccagtct ggaaccagac atgccgcaac atgtataaaa aatggttgct
     2641 gccttcctgc gaattatagc gattctgtat ttagatacca agctttttac tggaaatagc
     2701 cgctaaaaag ataagaaatg ctatggtttt catggactta ggcatggggg ttgatgtatt
     2761 ttttggcaac agtctaaata gggatttgta gcttgacagg catgaaataa aga
//
LOCUS       DSEB01000066            2548 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1005924, whole genome shotgun sequence.
ACCESSION   DSEB01000066 DSEB01000000
VERSION     DSEB01000066.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1005924"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(182..1813)
                     /locus_tag="ENO07_01245"
     CDS             complement(182..1813)
                     /locus_tag="ENO07_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00619.1"
                     /translation="MKKSVLVAITASLILSASALYACTNLLVTKGASADGSVMITYTC
                     DGEFHPHLEYTPAADNEEDSLEITDWGGNVRGKIHQVPHIYAVVDLMNEHQLAISETT
                     FTGREELQDTLGLLHYWDLMTLALQRAKTAREAIEVMTDLVKKYGYRSTGESFSIADT
                     DEAWIMEMNGMGPGNKGAVWVALRIPDGYISCHANKARIGEFPLDDPENCLYSENVID
                     FAVEKGFYDPESGQPFSFREAYCPDTPSNHKYADTRVWSIFRRAAPSQNFDPDYHRAV
                     VGAKEYPLWIKPDEKLSLSDVFSLMRDHYEGTPFDMTQGVDAGPYNTPYRWRPMEWEV
                     DSVTYMWERPISTQQTGFSFVSQSRGWLPDPVGGVFWYGVDDTWFTCYTPLYCGINDI
                     PKSYTVGSLQDFTWESAWWVFNFVSNFCNLKYSYMIQDIQAVQQELEGFYIKMQPRIE
                     QMALEISEFDNDLMIEFLTRYSVDNAENVVDRWRELGEYLIVKYNDGYVKNEKGRPEE
                     VGYPESWLEKIVRERGDYYRLPEQEEDVPESILID"
     gene            2078..>2548
                     /locus_tag="ENO07_01250"
     CDS             2078..>2548
                     /locus_tag="ENO07_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00620.1"
                     /translation="MSKRCLSSAMLKFAIAIMTLILLISFSFISSGAYAQEKISILIP
                     EDPTFHDLDSYLDIPVVMVNNTDINVGGFDFLIQYDTGAVELLDVNLGPVDHCNWALI
                     GHRPEIPGVEKIYAASGPNPLGCDFSYSTDTLFYLQFHIKPDTANECLTFPLRFY"
BASE COUNT          627 a          649 c          596 g          676 t
ORIGIN      
        1 attctacgca gatgtccgca tattcccgga tgcactatta tctgtggggc agggtcttga
       61 ttcgccgaag gcggattgtg accctgccag ccaagaaact ctatgctatt tttgattgcg
      121 ccagttcacc gccttcggtt caggaacagg cgcaaccagg atagcaaatg ctgcgtaaac
      181 atcaatcaat caatattgat tccgggacat cctcttcttg ttcaggcagc ctgtaataat
      241 cgccgcgttc acgcacaatt ttttccagcc agctttccgg atacccgacc tcctcgggac
      301 ggcccttctc attcttgaca tagccgtcat tatatttcac gatcaggtat tcgcccagct
      361 cgcgccatct atcgacgaca ttttcagcat tgtcaacaga gtagcgcgtc agaaattcga
      421 tcatgagatc attatcgaac tcgctgatct ccagcgccat ctgttcgatg cgcggctgca
      481 ttttgatata aaatccttcc agctcctgct ggaccgcctg gatatcctgg atcatatagg
      541 agtatttcag gttgcagaaa ttggagacaa agttaaacac ccaccaggcc gactcccagg
      601 tgaaatcctg aagcgatccc accgtgtatg atttcggaat atcgttgatc ccgcagtaga
      661 gcggagtata acatgtgaac caggtatcat cgaccccgta ccaaaaaaca ccgccgaccg
      721 gatcgggcag ccatccccgt gactgcgaga caaaagagaa tccggtctgc tgggtggaga
      781 tgggacgttc ccacatatag gtcacgctgt caacctccca ttccatcggg cgccatctgt
      841 atggcgtatt atatggcccg gcatcaacac cctgcgtcat atcaaagggc gtgccctcgt
      901 aatgatcgcg catcagggag aagacatcgg aaagcgacag cttttcatca ggcttgatcc
      961 agagcggata ttcctttgcg cccacgactg cgcgatgata atccggatca aaattctgag
     1021 agggggcggc gcgcctgaag atgctccaga cacgtgtatc ggcgtacttg tgattggaag
     1081 gtgtatccgg gcagtaggct tctctgaagc tgaacggctg gcccgattcg ggatcgtaaa
     1141 agcctttctc gacggcgaag tcgatcacat tttccgagta caagcaattc tcgggatcgt
     1201 caagcggaaa ctcacctata cgtgccttgt tggcatggca ggagatatag ccgtcgggga
     1261 tacgcaacgc cacccagact gcgcctttat tgcccggccc cataccgttc atctccataa
     1321 tccaggcctc atcggtatct gcaatcgaaa atgattcgcc ggtcgagcgg tagccatatt
     1381 ttttgaccag atcggtcatg acctcgatcg cctcacgcgc tgttttagcg cgttggagtg
     1441 ccagagtcat caagtcccag taatgcagca gccccagcgt gtcctgcaat tcctcgcgtc
     1501 cggtaaaggt ggtttcgctg attgccagct gatgctcatt catcaggtcc accacagcat
     1561 agatgtgcgg cacctgatga atcttgccgc gcacattgcc gccccaatcc gtaatttcaa
     1621 gcgagtcctc ttcattatcc gcagcgggag tatattccag atgtggatgg aattcgccgt
     1681 cgcaggtata ggtgatcatg actgagccgt cagccgaggc ccctttggtg accagaaggt
     1741 tggtgcaggc atagagtgct gatgccgata atattaatga tgctgttatt gcgaccaata
     1801 cggacttttt catatctgcc atctcctcaa tgaattgtcc attatatgat tacgatttca
     1861 aattgataaa tcagtacatc ctatccctga tgattttaag aaatttacat aagatgtccc
     1921 gattgcgcaa taagttcttg acaaatccag caaatgggat atgttttatt tgttagactg
     1981 ctacagcaaa gactaattat caagatagag gtttttcacg ggataaatgc tatcgtttta
     2041 ggcgttttaa tgctttaacc tgttaatggg aggaaatatg tctaaacgat gcctgtcaag
     2101 tgctatgctt aaatttgcca tagcgatcat gactttaatt cttcttattt cgttcagctt
     2161 tatatccagc ggcgcatatg cccaggaaaa aatcagtatc ttgatacctg aggatccaac
     2221 atttcatgat ctggacagct accttgatat cccggtcgta atggttaaca ataccgatat
     2281 caatgtcggc ggcttcgatt ttcttatcca gtatgatacg ggtgcggtgg agctgctcga
     2341 tgtaaatttg ggtcctgttg accactgtaa ttgggccctg attggacatc ggcctgagat
     2401 acccggggtg gaaaaaatat atgccgcatc cggcccgaat ccgctcggat gcgatttttc
     2461 ttacagcacc gacacactct tttatctgca gtttcacatt aagcccgata ccgcaaatga
     2521 gtgcctgact tttccattaa gattttac
//
LOCUS       DSEB01000067            2358 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1007024, whole genome shotgun sequence.
ACCESSION   DSEB01000067 DSEB01000000
VERSION     DSEB01000067.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1007024"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(71..1867)
                     /locus_tag="ENO07_01255"
     CDS             complement(71..1867)
                     /locus_tag="ENO07_01255"
                     /inference="COORDINATES: protein motif:HMM:PF00005.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDS00621.1"
                     /translation="MRDKIKWLWKYYKRYPYLLLVLLILTPVQAAFQVAIPRMVEFTV
                     DFVKTGTVQGGIQIWLTGLGSDLGLSAAATYALTLVALGVIACALYAFVQSHRAWMNF
                     KLEWMIRQDSFNDITRKGPDFFNKFRTGDLVTRLSDDIEQKLAWFACSGIFRFYEAVV
                     FVVFTLAMMITIDPMLTLLSAGPLPILILIFFKSATILDRRYDNLQRNISSFNDIMEA
                     CFSGIRVVKAYVKEKVQKKKFHEAAVNRRAAEISAIKATTIVDSLYMYIWQFGVVIVL
                     LAGGYMVIGANLSIGKLVAFVYYVVYLIFPMFDIGQFLVKSRQSAVSINRLRELQAAP
                     AMVDENGSVGADGDFRGAIEYRNVSFGFPDSARNIVDDISMKISPGETVALVGKVGAG
                     KSWMVNMIPRLVDPSEGEIRIDGHKLSDYRLSQLRRQIGYVPQEPVLFSDTVRNNILF
                     GREYISEEVMNWAIEVSQLKSEIDEFPDGINTYIGTRGVSISGGQKQRLALARALAGK
                     PKILILDDCTSALDSSTESALWARLHEVMPDMTTILITHRPDTLESADMIYVLEDGKI
                     VETGKHAELIENGGQYAKMYRRYRLAEEVTYG"
     gene            complement(1857..>2358)
                     /locus_tag="ENO07_01260"
     CDS             complement(1857..>2358)
                     /locus_tag="ENO07_01260"
                     /inference="COORDINATES: protein motif:HMM:PF00005.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDS00622.1"
                     /translation="DILLFPDTVKANIALDEPGVTDERVVQAAKIVEADHFIRRLPNG
                     YDTEVSEKGANFSRGERQLLSFARALVTNPDILLLDEATSSVDPETERAIQESLRKLM
                     KGRTSLIIAHRLSTILDVDEILVIRRGEIIERGTHTDLILQNGYYSKLFHLQFKNKNG
                     VLKDAG"
BASE COUNT          550 a          672 c          580 g          556 t
ORIGIN      
        1 gcttcatccg gaccgtttga aggccacact cagatattaa aaagcccggg tttttactcc
       61 cggacttatc ttatccgtac gtaacttctt cggccaggcg gtagcgccga tacatttttg
      121 catactgccc gccgttttcg atcagctcgg catgtttgcc ggtctccacg atttttccat
      181 cttccagcac gtagatcata tcggcgctct ccagagtgtc cggccggtgg gtaattagaa
      241 ttgtcgtcat atccggcatg acctcatgca gccttgccca cagggcgctc tcggtgctgg
      301 aatcgagggc cgaggtgcaa tcatccagaa tcagtatctt gggcttgccg gccaatgcac
      361 gagccagcgc cagcctctgt ttctggccgc cggagatcga aacaccgcgt gtcccgatat
      421 atgtgttgat gccgtcgggg aactcatcga tctccgattt caattgcgaa acctctatgg
      481 cccagttcat gacctcctcg gagatatact ctctaccgaa aaggatgttg ttcctgaccg
      541 tatccgagaa cagcacaggc tcctggggca cataaccgat ctgcctgcga agctggctga
      601 gcctgtaatc gcttagctta tgcccgtcaa tcctgatttc accttctgag ggatccacca
      661 ggcgcggaat catattgacc atccagcttt ttccagcccc gaccttgccg accagcgcaa
      721 cagtttcgcc cgggctgatc ttcatagaga tatcatcaac aatattccgc gcagaatccg
      781 gaaatccgaa ggacacattc cggtattcaa ttgcgcccct gaaatcgccg tcagccccga
      841 ccgagccgtt ttcatcaacc atggccggag ccgcctgcaa ttcccggagg cgattgatcg
      901 aaaccgctga ctgtcgtgat tttaccagaa actgcccgat gtcgaacatg gggaagatca
      961 ggtagacgac ataatataca aaggccacca gcttgccaat cgacaaatta gccccgatta
     1021 ccatatatcc tcccgccagg agcacaatta ccacgccgaa ctgccagata tacatgtaga
     1081 gcgagtccac aatggtcgtg gccttgatgg ccgatatttc ggctgcacgt cggttgacag
     1141 ccgcctcatg gaacttcttc ttctgcacct tctccttgac gtaggccttg accacccgga
     1201 ttcctgagaa acaggcttcc atgatatcgt tgaagctgga gatatttctc tgcaggttgt
     1261 cgtaacgccg gtcgagaatg gtggcgctct taaagaagat caggatcaga atcggcagcg
     1321 gcccggccga caggagtgtc agcatggggt cgatggtgat catcatggca agggtgaaga
     1381 ccacgaagac caccgcttcg tagaacctga atatccctga acacgcgaac catgccagct
     1441 tctgctcgat atcatccgag agacgtgtca ccaggtcacc ggtccggaac ttattaaaga
     1501 aatccggacc cttgcgggtg atatcgttga atgaatcctg gcgaatcatc cattccagct
     1561 tgaagttcat ccaggcccga tgcgactgca cgaatgcgta cagggcacag gcgatgactc
     1621 ccagggctac cagtgtcagg gcataggtgg ccgcagcgga cagccccagg tcagatccca
     1681 aacccgtcag ccatatctga attccgccct gaacagttcc ggtcttgacg aaatctactg
     1741 taaactcaac cattctggga atagctacct gaaacgctgc ctgaaccggt gtcagaatta
     1801 ataacaccag caatagatag gggtaacgtt tgtagtattt ccagagccat tttattttat
     1861 cccgcatctt tcaatactcc gtttttattc ttgaactgca gatggaacag cttggaataa
     1921 taaccattct gcaggatcaa gtctgtatgg gtgccgcgtt cgataatctc tccgcggcgg
     1981 ataaccagga tctcatcgac gtccaaaatg gtcgagagac ggtgcgctat gatgagcgag
     2041 gtccgtccct tcatcagctt gcgcagcgat tcctgaatag cgcgctctgt ctccgggtcg
     2101 accgagctgg tggcctcgtc cagaagcaaa atatccggat ttgtaaccaa ggctctggca
     2161 aatgacagga gctgacgttc cccgcggctg aagttggcgc ctttttctga cacctcggta
     2221 tcatatccgt tcgggagcct gcggatgaaa tgatccgctt cgactatctt tgccgcctgc
     2281 acgacccttt catcggtaac gcccggctca tccagcgcaa tattggcctt gaccgtatcc
     2341 gggaagagca gtatatcc
//
LOCUS       DSEB01000068            5797 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000953, whole genome shotgun sequence.
ACCESSION   DSEB01000068 DSEB01000000
VERSION     DSEB01000068.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5797)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000953"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            319..1563
                     /locus_tag="ENO07_01265"
     CDS             319..1563
                     /locus_tag="ENO07_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008189037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDS00623.1"
                     /translation="MTASQMREMQKFSSDPEYKSAIDQLYHSPPVAKSNIILKLLFLM
                     VSLGLMTAIGVKAMGHNPFEYLAGYVVIFVLAIIAYTFSDQNVIKAYGLGYAFWALLL
                     GLLISNTIGTPWWLLTGARTEMFIKTGLVLLGAEILFGKILALGAPGLFVAWLVTPTV
                     IIFMFLFGTRILNMASKTLVIIIAAATSVCGVSAAIATAAACKAKKDELTLAVGMTLI
                     FTVLMMIFMPIAIKMVGMKTAVGAAWMGGTIDATGAVVAAGSMLGAEAEKIAAVVKMI
                     QNILIGVVAFVVAVYWAFRIERTADAPRANAMEIWYRFPKFIVGFIGASIVFSFVLIP
                     IYGSNYIESEIISASTKTIRGWLFCIAFVSIGLESNFRDLGSQLVGGKPILLYICGQS
                     FNLVLTLFAAYLAFGGILFGKI"
     gene            1550..1768
                     /locus_tag="ENO07_01270"
     CDS             1550..1768
                     /locus_tag="ENO07_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00624.1"
                     /translation="MERFKKIVKFSAGCVVLGLILLAAASGNIPTGGEGGVIEHNLRE
                     KRDATPLIYSESEEMPRLEQGLSEIKLR"
     gene            complement(1831..2676)
                     /locus_tag="ENO07_01275"
     CDS             complement(1831..2676)
                     /locus_tag="ENO07_01275"
                     /inference="COORDINATES: protein motif:HMM:PF03692.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDS00625.1"
                     /translation="MRMAQREIQKLKETILKEYPRLKKDDKFKFACHSGVSCFNECCG
                     DVNIFLTPYDVLRLKNTLGISSQEFLDKYTILPIDQNQRLPVVLLKMQDNEKKTCYFV
                     SKEGCTVYENRPWACRMYPLGLASPGELAANLNEEFYFILKESVCKGFNENREWTVRE
                     WIEDQGIAEYDKFGEMYKQIYTHPYLEKAPELTPQKIDMFFLACYNLDKFRDFILHST
                     FLQKFDVEDDLLEKIKTDDEELLKFGFRWVRFALFGEKTIDIKDDVKAMAEKKLEEKR
                     RKTAK"
     gene            complement(2642..2953)
                     /locus_tag="ENO07_01280"
     CDS             complement(2642..2953)
                     /locus_tag="ENO07_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00626.1"
                     /translation="MTEEKAVDKTVCPHCGHKMKKWKVPPESTWTNEYHWICFNDDCP
                     YYRKGWKWMEEKYEQRASYRHSYNPETGQTGPIPVWSPNALKSGILEEDEDGTERNSE
                     T"
     gene            complement(3081..4952)
                     /gene="aprA"
                     /locus_tag="ENO07_01285"
     CDS             complement(3081..4952)
                     /gene="aprA"
                     /locus_tag="ENO07_01285"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate reductase subunit alpha"
                     /protein_id="HDS00627.1"
                     /translation="MADFETKEITTDVLILGGGMAASGAAVEAAYWAKKNGLQVALVD
                     KAAMDRSGAVAMGLSAINQYVGLKDGQNTVKDYVDYVKNDLMGIARDDLVASIARHVD
                     STVHLFEKWGLPIWKDEEGAYVHEGRWQLMINGESYKVIVAEAAKNAVGTDNIYERVF
                     ITEPLVADGRCVGAVGFSVRENIFYVFKAKAVLAAMGGAVHVFKPRSAGEGLGRAWYP
                     PWNSGSSAYFTLKAGAEMTCQEVRFIPVRFKDAYGPVGAWFLLFKSRATNAQGEAYME
                     TRRDELEKWKPYGLVKPIPANLRNWLMMLDVMEGKGPIYMRTEEAIQNIAKSEGDEKA
                     AKKKLKELEAEAWEDFLDMTISQAILWAATNTAPEEKPSEIAAAEPYFIGSHSGASGA
                     WVSGPADIAPEEYQWGYTNMSTVKGLFCAGDASGASSHKFSSGSHAEGRIAAKAACRF
                     ALDNADIPEVDAGKIEEMKKTILAPLDKFAQNSKDSSDPDINPAYMKPKMFMFRLQKI
                     MDEYAGGVTAQFSTSKAQLEKGLELLTFLKEDAEHLAAEDLHELMRCWENVHRMWQAE
                     AHVRTILAREETRWPGYYFRSDTPQMKEDWEVFVNCVWHPDSGEWEMKKVPIKRLFE"
     gene            complement(4971..5405)
                     /gene="aprB"
                     /locus_tag="ENO07_01290"
     CDS             complement(4971..5405)
                     /gene="aprB"
                     /locus_tag="ENO07_01290"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015591666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate reductase subunit beta"
                     /protein_id="HDS00628.1"
                     /translation="MPSYVIPEKCDGCKALDKTACQHICPNDLMVLNKDIMKAYNREP
                     EMCWECYNCVKICPTQAIEVRGYADFVPMGATVTPMRSSDSILWTVKFRNGMLKRFKF
                     PIRTTSEGTAEPEGGYSTEPDLNSEVLFTEPDSIGMDQMWTK"
     gene            complement(5470..>5797)
                     /gene="sat"
                     /locus_tag="ENO07_01295"
     CDS             complement(5470..>5797)
                     /gene="sat"
                     /locus_tag="ENO07_01295"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697911.1"
                     /note="ATP sulfurylase; ATPS; converts ATP and sulfate to
                     5'phosphosulfate and pyrophosphate; in some organisms this
                     enzyme is involved in the incorporation of inorganic
                     sulfate while in others it is involved in the production
                     of ATP in the reverse direction; the enzyme from Thermus
                     thermophilus is dimeric and binds a zinc ion that is
                     coordinated by cysteine and histidine residues that are
                     not found in all related proteins but is found in some
                     thermophilic organisms; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HDS00629.1"
                     /translation="FDAQKIFHELWDGALECKMLPIDWTFWCYKCGGMASMKTCPHGK
                     EDRLLLSGTMLRKALSEGGEVPAEFSRTEVLALLREYYASLKDEDRVEVKLHGAATGD
                     VKKDKK"
BASE COUNT         1315 a         1490 c         1445 g         1547 t
ORIGIN      
        1 atatatatct taataccgga atcctgcttt ttaagggagg tatggatgag caataattcc
       61 ggattaagat cacttattag aagcgaggat tggtgggcgg tatggttcgg tgcagctata
      121 atcatagttg cccttctgca ttttaccggc aaggctccca aactcgggga atgggttaaa
      181 aatcctctaa accagttcga gagctatgaa cgtgtttacc cgctcgaaaa taagcctgcc
      241 gatcttaata tcgagggccc gctttcaaaa catcttaaat acgatgaaaa acaaggggtc
      301 ctgatttaca agggcttgat gactgcgagc cagatgcgtg agatgcagaa attctcttca
      361 gatcctgaat acaagtcggc aatcgaccag ctttaccaca gcccgcctgt ggctaaaagc
      421 aatataattc tgaaactgct tttcctgatg gtctcgctgg gcctcatgac tgcaatcggc
      481 gtgaaggcga tgggacataa tccgtttgaa tacctggccg ggtatgtcgt gatttttgtg
      541 ctggccataa tagcctatac gttttccgat caaaatgtga tcaaggccta tggtctgggg
      601 tatgccttct gggcactgct cctgggtctc ctgatctcca atacgattgg cactccctgg
      661 tggctcttaa ccggggcgcg gactgagatg tttataaaaa ccgggctcgt ccttctgggc
      721 gcggaaattc tttttggcaa aattctggct ctgggcgcgc caggactttt tgtcgcctgg
      781 ctggtaactc cgactgtgat aattttcatg ttcctgttcg gcacccgaat tcttaatatg
      841 gcttccaaga ccctggtgat aatcattgcc gcggcaacct cggtctgcgg tgtgtcggcg
      901 gcaattgcaa cagcggccgc ctgcaaggcc aaaaaggatg aactcaccct ggcggtaggg
      961 atgaccctga tattcacagt cctgatgatg attttcatgc cgattgcaat caagatggtg
     1021 ggcatgaaaa ctgctgtcgg tgcagcctgg atgggcggta ctatcgacgc taccggggcg
     1081 gttgtggcgg ccggatcgat gctgggcgcg gaagccgaga aaatcgccgc ggtggtcaaa
     1141 atgattcaga atattctgat cggcgtggtt gctttcgttg tggcagtata ttgggccttc
     1201 agaatcgagc gcaccgccga tgctccccgt gccaatgcca tggaaatctg gtaccgcttc
     1261 ccaaaattca ttgtgggatt tattggagca tccatcgtat tttccttcgt actcataccg
     1321 atttacggct cgaattatat tgaatcagaa attattagcg catcaacaaa gaccattcgc
     1381 ggatggctgt tctgcatcgc cttcgtatcg atcggcctgg agtcaaattt ccgagatctt
     1441 ggaagtcagc ttgtgggagg caaaccgatt cttttgtata tctgcggaca gagttttaac
     1501 cttgtgctga ctttatttgc ggcctatctg gcgtttggag gaatcctctt tggaaagatt
     1561 taagaagatc gttaagttct cggccggatg tgttgtgctt ggacttatcc ttctggccgc
     1621 agcatccggg aatattccaa ccgggggaga gggaggtgtg atcgagcata acctcaggga
     1681 aaaaagggat gccaccccgc tgatatattc ggagagcgag gagatgcccc gcctcgaaca
     1741 gggcctttcc gaaataaaac tgagatgaat agcataaata aaaaagcagc ttcagtcatg
     1801 gagctgcttt aaaaatgcgg gaataatttt ttatttggct gtcttcctgc gcttctcctc
     1861 gagtttcttt tcggccatag ctttgacgtc atccttaata tcgatggttt tttcgccaaa
     1921 aagcgcaaac ctgacccacc ggaagccgaa ttttaacagc tcttcatcat cggtcttgat
     1981 tttttccaga agatcatcct cgacatcgaa tttctgcagg aaagtgctgt ggaggatgaa
     2041 atccctgaac ttgtcgagat tataacaggc caggaaaaac atatcgattt tctggggagt
     2101 cagctcagga gctttctcga gatatgggtg cgtgtatatc tgcttataca tctcgccgaa
     2161 tttatcatat tccgctatac cctggtcctc gatccattcc cgaaccgtcc actccctgtt
     2221 ctcattgaat cccttgcaga cagattcctt cagaatgaaa taaaattctt cattcagatt
     2281 tgcggccagt tcgccgggtg aggccagacc caggggatac attctacagg cccagggacg
     2341 gttttcataa actgtacatc cttctttcga tacaaaatag caggtcttct tctcattatc
     2401 ctgcatcttg agcagcacca ccggtaatct ctggttctga tcgatcggaa ggatagtgta
     2461 cttgtccaga aattcctgtg acgatattcc cagggtattc ttgagcctga gcacatcata
     2521 aggtgtaagg aaaatattca catcaccgca gcattcattg aagcacgata ctccggagtg
     2581 acatgcgaat ttgaatttgt catccttttt caagcggggg tattctttaa gtatggtctc
     2641 tttaagtttc tgaatttctc tctgtgccat cctcatcctc ttccaatatt ccgcttttta
     2701 aagcattcgg cgaccagacg ggaatcggcc cggtctgacc ggtttcagga ttatagctgt
     2761 gacggtagga agcccgctgt tcgtactttt cctccatcca cttccagccc tttctgtaat
     2821 aggggcaatc gtcattgaaa cagatccaat ggtattcatt ggtccacgtg gattcgggag
     2881 ggactttcca cttcttcatc ttgtgcccgc aatgcgggca gactgtctta tcgactgctt
     2941 tttcttcggt catcaacatc acctttatac gcactgtatc caaatattaa ttgcatctta
     3001 gattgtcttt aattagaaat cccccgggag tgtttctccc ggaggattat tctatcaaag
     3061 aagtctaagc gagttgtctt ttattcaaac agacgcttga tcggaacctt cttcatttcc
     3121 cattcgccgc tgtcagggtg ccacacgcag ttgacgaaga cttcccagtc ctctttcatc
     3181 tgaggagtat cggaacggaa gtagtaaccc ggccagcggg tttcttcacg ggcgaggatt
     3241 gtgcgcacat gcgcttcagc ctgccacata cggtggacat tctcccagca acgcatcagt
     3301 tcgtgcagat cctctgcagc caggtgctcg gcatcttcct tcaggaaggt gaggagctcc
     3361 agacccttct cgagctgggc cttggaggtc gagaactgag cggtaacacc gccggcatac
     3421 tcgtccataa tcttctgcag acggaacatg aacatcttcg gcttcatata ggccgggttg
     3481 atgtcgggat cggatgagtc tttgctgttc tgagcaaact tatccagcgg agccagaata
     3541 gtcttcttca tctcttcgat tttgccagca tcaacttcag gaatatctgc attatcgagt
     3601 gcgaatctgc aggctgcttt agcggcgata cggccttcag catgcgatcc ggaggagaac
     3661 ttgtgcgagg aggcgccgga ggcgtcgcca gcacagaaga gacccttcac ggttgacatg
     3721 ttggtataac cccactgata ttcttcgggg gcgatatcgg ccggaccaga aacccaggca
     3781 ccggaggcgc cggagtgtga gccgatgaag tagggctcgg ctgcggcgat ctctgagggc
     3841 ttctcttccg gagcggtgtt tgtggcagcc cagaggatag cctgtgagat tgtcatgtcg
     3901 aggaagtctt cccaggcttc cgcttcgagc tctttcagtt tcttcttggc ggccttctcg
     3961 tcgccttctg atttggcaat attctggatc gcttcctcgg tgcgcatata gatcgggccc
     4021 ttgccttcca taacgtcgag catcatgagc cagttgcgca ggttggccgg gatcggctta
     4081 accagaccgt agggtttcca tttctccagt tcatccctgc gggtttccat gtaggcttca
     4141 ccctgggcgt tggtggcacg gctcttgaag agcaggaacc atgcgcccac cgggccgtaa
     4201 gcatccttga aacgtaccgg gatgaaacgg acttcctggc aggtcatctc agcgccggcc
     4261 ttcagcgtga agtaggcgga ggaaccggag ttccacggcg gataccaggc gcgtcccagg
     4321 ccctcaccgg ctgaacgcgg cttgaagacg tgaactgcgc cgcccatcgc ggccaggact
     4381 gctttagctt tgaaaacgta gaatatgttt tcacgaaccg agaagccaac cgcgccgaca
     4441 catctgccat cggcaaccag cggctcggta ataaacacac gctcatagat attatcggtg
     4501 ccgacagcat ttttggcggc ctcggccacg ataaccttgt aggactcacc attgatcatg
     4561 agctgccagc gtccttcatg aacataggcg ccttcttcgt ctttccagat cgggagtccc
     4621 catttctcaa acagatgaac tgttgagtcg acatgacggg cgatcgaggc gaccaggtcg
     4681 tcacgagcaa tacccatcag gtcattctta acataatcga cataatcctt aacggtattc
     4741 tgaccgtctt tcaggccgac atactggttg atagcggaca ggcccatagc gacagcgccg
     4801 ctgcggtcca tagctgcttt atccaccaga gcgacctgga ggccattctt ctttgcccag
     4861 taggcggcct caacggcagc accagaagcg gccatgccgc cacccaggat cagcacatcc
     4921 gtggtaattt ctttggtttc gaaatctgcc atctttttcc tatcctttgt ttacttggtc
     4981 cacatttgat ccatgcctat agagtcaggc tcggtgaata aaacttcgct gttgagatca
     5041 ggctcggttg aataaccgcc ttcaggctca gcggtgccct cagaggttgt cctgatcggg
     5101 aatttgaaac gcttcagcat gccatttctg aacttcactg tccagaggat ggaatctgag
     5161 cttctcattg gagtaacagt tgcgcccatc ggtacgaaat cggcgtaacc gcgtacctcg
     5221 atcgcctgcg tggggcagat cttaacgcaa ttgtagcact cccagcacat ctctggctcc
     5281 cgattatagg ccttcatgat gtctttgtta agcaccatca ggtcattcgg gcagatatgc
     5341 tgacaagctg tcttatcaag tgccttacat ccgtcgcact tttcagggat tacatagctt
     5401 ggcattaagt cctccttaat gaactgtaat gtgtctctgc catcttctaa cgagacaccg
     5461 tgatttaatt tatttcttgt ctttctttac atcgccggtg gctgcgccgt gcagcttgac
     5521 ctcaactcta tcctcgtctt taaggctggc ataatactcg cgcaggagag ccagaacttc
     5581 cgtacggctg aattccgccg gaacctctcc gccctcagaa agcgccttgc gcagcatagt
     5641 acccgagagc aagagcctgt cttccttgcc gtgcgggcag gtcttcatgg aagccatgcc
     5701 gccgcatttg tagcaccaga aagtccagtc gatcggaagc atcttgcact ccagagcgcc
     5761 atcccatagc tcgtggaaaa tcttctgggc atcgaag
//
LOCUS       DSEB01000069            3696 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002569, whole genome shotgun sequence.
ACCESSION   DSEB01000069 DSEB01000000
VERSION     DSEB01000069.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002569"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            56..562
                     /locus_tag="ENO07_01300"
     CDS             56..562
                     /locus_tag="ENO07_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00630.1"
                     /translation="MGKKSKNMAIILLMSGIIIISAVSVSAQDSLVIEKRKRHSPTGA
                     LLRSLVIPGWGQLYNDKYIKAGLIAGLESFLIYQTAYYWDKTSTYEDLYTSDTNSVSR
                     YDKFLQFDRYRDLRNQHIWFLGITVVYSIFDAYVDAHLKNFDVDITPDFDENRQDLTL
                     WLNLCIKY"
     gene            816..1490
                     /locus_tag="ENO07_01305"
     CDS             816..1490
                     /locus_tag="ENO07_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00631.1"
                     /translation="MNDKPVFSIKDCALITRMAGLDSALNIREMRERVAVCPIECLYH
                     HFCETVIRPTFDDPEFRNDFSIWCSTNLGEKKLAEQLGILNPYDFNNLEELRAKMMEI
                     IDERLSELYYIPSAQIGEDFQFMRAVTVVFDTGKQIHSPDDLIRHIPFMSDSSLYYHF
                     VEAHRRTPESTDDFTVFLKDFNHEYQPLIEEFARIDFYFLNLHELRLALKKAIESIVG
                     AEVPGA"
     gene            1483..2721
                     /locus_tag="ENO07_01310"
     CDS             1483..2721
                     /locus_tag="ENO07_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDS00632.1"
                     /translation="MPKSLLDYEEIVGKINISILRQLAENLKGARVVHVNSTRVGGGV
                     AEILDWLISLMNELGLEASWEVISGPPEFYFTTKTFHNALQGKPASLTKTMVEAYEET
                     NRQTADKLRGTLEDADFVFVHDPQPAALLEYCPNRKGKWIWRCHIDASRPYRRVWKYL
                     EEKVEKYDASIFSMPSFAQRLPHPQFIIAPSIDPLSNKNCELDPTEIRSALETYGIEN
                     SKPVLTQISRFDRFKDPIGVIQAFNMLRETIDADLILAGGGASDDPEGQMVLEEVLEA
                     ANGDRQIHILNLPGDAHRMINALQRASTIVLQKSLQEGFGLTVTEALWKEKPVIGGNV
                     GGIRLQVHNHHTGFLVNTPEGAALRMRYLLRHPEELEYMGKTGKRFVQENFLLTRHLR
                     EYLTLMLGLRRGLANYLITE"
     gene            2732..3520
                     /gene="otsB"
                     /locus_tag="ENO07_01315"
     CDS             2732..3520
                     /gene="otsB"
                     /locus_tag="ENO07_01315"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HDS00633.1"
                     /translation="MQVLRDNFDLDQFFSDLSGAIKPILLLDYDGTLAPFQVKRDEAV
                     PYPGVREILNQILRADHTHILVISGRRVDDLTALLKLEKQPEIWGSHGNERLLRDGRY
                     ILSKLSERESRGLDLARKWAKEQGLDANLEEKPASLAFHWRGLDKSRADEIRKNVRDT
                     WEPVKDYYNLKLLDFDGGIEIKVAGRNKGDAVQRILTEIDEIEEVAYLGDDFTDEDAF
                     RALQGKGLRVLVRQERRETLADLWIIPPDELLYFLRRWHEARSR"
BASE COUNT          953 a          827 c          940 g          976 t
ORIGIN      
        1 acaaaaatta cagttttata gtttatacac atttattatt gtataatcag gtgaaatggg
       61 aaaaaaatcg aaaaatatgg cgattatact cctgatgtcg gggataatca ttatttccgc
      121 tgtctctgtg tccgctcagg acagtttagt aatcgagaag cgcaaaaggc actcccccac
      181 aggtgcatta cttcgttcgc tggttattcc cggctggggc cagctctaca atgataaata
      241 tatcaaggcc ggattaattg ccgggctgga atcattttta atctatcaga ccgcctatta
      301 ctgggataag acctccacct atgaggatct ctataccagc gacaccaact ctgtcagcag
      361 gtatgataaa tttctgcagt ttgaccgtta tcgcgacttg cgcaatcagc atatatggtt
      421 tttgggaata accgtcgttt attcaatttt cgatgcttat gtggatgccc acttgaagaa
      481 tttcgatgtt gatattacgc ccgattttga tgaaaatcgg caggatttga ccctgtggct
      541 caatttgtgc attaaatatt agtcccgcat tgtcgcagat tctgcatttt gatcccctaa
      601 tttttgatta tggcttgcct cctttcggca tggaactata ttgcgcagaa ttgttatact
      661 atttggcgaa ttccaaaagg ggaccgtatt ttcgggatat gcagctttgg ttatcctaaa
      721 ctgcgataaa gtgctgttga ttacatacga taagtaaggt atggggtata ttttgcggga
      781 atgttgcccg ctttggatga aataggattt aatgaatgaa cgacaagcca gtattcagca
      841 ttaaggactg cgccctgatc acccgaatgg ccggcctgga ttcggccctt aacattcgcg
      901 aaatgcgaga acgggtggcg gtttgtccta ttgaatgtct ctaccatcat ttctgtgaaa
      961 cggtaatccg cccgacattt gatgaccccg agttccgcaa cgatttctcc atctggtgct
     1021 ctaccaatct gggggagaag aaactggcag agcagctcgg aatattgaat ccctacgatt
     1081 tcaataatct ggaggagctg cgggcgaaga tgatggaaat catcgatgaa cgtttgagcg
     1141 agctttatta tattccctcg gcccagatcg gcgaggattt tcaattcatg cgggccgtaa
     1201 ctgtggtatt cgataccggc aagcagattc attctcccga cgacctgatc cgccatattc
     1261 cctttatgag cgacagcagc ctttattatc attttgttga ggctcaccgc aggactcccg
     1321 aaagtactga cgactttacc gtatttctga aggatttcaa tcatgaatac cagcccttaa
     1381 ttgaggaatt cgcgagaata gatttttact ttttgaatct tcatgaactc cgtttagcct
     1441 tgaagaaggc tatcgagtca attgtcggcg cggaggttcc tggtgcctaa atcgctactg
     1501 gattatgaag agatagttgg taagatcaat atctcgatcc tgcgtcaact ggcggaaaat
     1561 ttaaagggcg ccagggtggt gcatgtcaac tcgacccgtg tgggcggggg agtggcggaa
     1621 atactggact ggctcatctc cctcatgaat gagctgggcc tggaggcatc atgggaggtg
     1681 atctcgggac ctccggaatt ctattttacc accaagacat ttcataatgc tctgcagggc
     1741 aaacccgctt cccttaccaa gacaatggtt gaggcctatg aagagaccaa caggcagact
     1801 gccgataaac tgcggggtac gcttgaagat gcggatttcg tatttgttca tgatcctcag
     1861 ccggcggctc tactggagta ttgtcccaat cgcaagggaa agtggatctg gcgctgtcac
     1921 attgacgcca gcaggcccta caggcgggtc tggaaatacc tggaggagaa agtggagaaa
     1981 tatgatgcca gtatcttttc catgcccagc ttcgcccagc gccttcccca tccgcaattc
     2041 atcatcgcgc ccagtattga cccgctctcg aataaaaact gcgaactcga tccgaccgag
     2101 ataagatcgg ccctggaaac ctacggtatc gaaaattcaa aaccggtcct gacccagata
     2161 tcccgttttg accgtttcaa agaccccatc ggagtcattc aggctttcaa tatgctccgg
     2221 gagacgatcg atgccgacct gattttagcc ggaggcggcg ccagcgatga cccggaggga
     2281 cagatggttc tggaagaagt cctggaggcg gccaacggcg acaggcagat tcatatcctg
     2341 aatcttcccg gggatgcgca caggatgatt aacgcccttc agagggcatc gaccatagtc
     2401 cttcaaaagt ccctgcagga gggtttcggg ttgaccgtga ctgaggctct gtggaaggaa
     2461 aaaccggtta tcggcggaaa tgtgggcgga atcaggctgc aggtgcataa tcatcacacc
     2521 ggttttcttg taaatacccc cgagggcgcg gccctgcgca tgcgttacct tctcaggcat
     2581 ccggaggaac ttgaatatat gggcaaaacc ggaaaacgct tcgtgcagga gaattttctg
     2641 ctaacccgtc atctgcgtga atacctcact ttgatgcttg gtttgagaag agggcttgcc
     2701 aactacctga ttactgagta aattaccgca aatgcaggtt ttaagggaca acttcgattt
     2761 agatcagttc ttctccgatc tcagcggagc cataaagcca attcttctgc tggattatga
     2821 cggtactctt gcccccttcc aggtaaaaag agacgaggcc gttccctatc ccggtgtgag
     2881 agagatcctg aatcagattt tgagagcaga tcatacccat attctcgtca taagcggcag
     2941 gcgtgtggac gatttgaccg cattgctgaa actcgaaaaa cagcccgaaa tctggggctc
     3001 gcatggaaat gagaggctgc tccgagatgg gagatatatt ctgtcaaaat tgtctgaacg
     3061 agaatcccgg ggtctggatc tggcccggaa gtgggcaaaa gaacagggtc ttgacgctaa
     3121 tctggaggag aagcccgcaa gtctggcctt tcactggcgc ggattggaca agagccgggc
     3181 cgatgaaatc aggaaaaatg tccgggacac atgggagcct gtaaaggatt attataatct
     3241 caagctgttg gattttgatg gcgggatcga aataaaggtt gccggacgga ataaaggtga
     3301 tgcggtgcaa agaattctga cggaaatcga tgaaatcgag gaagtcgcct acctgggcga
     3361 tgattttact gatgaggatg cttttagggc gttgcagggc aaggggctac gtgttctggt
     3421 aaggcaggag cggcgcgaaa cgttggctga tctgtggata atcccgcccg atgagctcct
     3481 ctatttcttg cgacgttggc atgaagctcg cagcagatag atgccataaa atttttttta
     3541 acgaacaata atttttaagg ggcgttacat ttagtaaagg gttatattac gatttagaag
     3601 tgattcgaat gccattttac atctttatgc atagtcgtcc tccggcattg acctgacagc
     3661 aacatttttt agctctttta cagtcatatt tatttg
//
LOCUS       DSEB01000070            2936 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004302, whole genome shotgun sequence.
ACCESSION   DSEB01000070 DSEB01000000
VERSION     DSEB01000070.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004302"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..725
                     /locus_tag="ENO07_01320"
     CDS             <1..725
                     /locus_tag="ENO07_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDS00634.1"
                     /translation="ILISVPSGITEVEKRAVRDSAENAGAREVYLIHEPMAAAIGVGL
                     PVDQPSGIMIIDIGGGTSEIAVIALYGIVNNRSIRIAGDEINEAIVIYLKKNYNLLIG
                     ELTAEEIKMKIGSAFPMEREESVEIKGRDLVAGVPKNMKISSAQVREALTEPIEAIIE
                     AVRLSLEATPPELASDILDRGIVLTGGGALLRGFAKRIRHETNLPVYVADDPMTCVAR
                     GTGTVLENMNAFSKVLIKSRND"
     gene            806..>2936
                     /gene="rnr"
                     /locus_tag="ENO07_01325"
     CDS             806..>2936
                     /gene="rnr"
                     /locus_tag="ENO07_01325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDS00635.1"
                     /translation="MDITAEKIVAYVSKENYRPVRPRELAKEMKIPEKNYRKFRNLLK
                     NLISDGELVKIRGGRIGPPEKMNLKVGKIQITSKGFGFLMPDDGKEEIYIRANDTKTA
                     LNGDRVVVRVKPYRTPGKKSEGEVVKVLERARNTIVGTYHSSKYFEYIEPDDPSFKRD
                     VYIMPDHGLSPRDGQKVAVILKEWKNQFLNPEGKLVEVLGFPHEAGVDVLSVIKKFEL
                     PLEFQAPVLREASKVKINITPAEIKKRRDYRDQITFTIDPVDAKDFDDALSIRKNSSG
                     NYLVGVHIADVSHYVGEGSSLDKEARNRGTSVYLVDRVLPMLPEHLSNEICSLKGGED
                     RLTYTCEMEINDKGDVVKFEIFKSIINSSARLDYDQVQEYFDTGGTDSVPDNVARALE
                     PLRKIARKVRQRRFQKGSLDFDLPEPLVIMDQSGEVVDIQVRPRKESHRLIEEFMLLA
                     NKCVARHFVRLGLPTLFRVHDRPDKDKIEAFQEFAKSFGYEFSFPDPVKPKHLADSIR
                     EFEGTPEEDLLNEILLRSLKKAQYQRENIGHFGLAFENYLHFTSPIRRYPDLMVHRLL
                     SEIKNKKYPGTRTASVNEFLDRVGKHSTDMEIIAEKAERETIKIKQVLYLSRQIGNIY
                     QGIISGMTSGGFFVRLMQFSAEGMVRLSMMVDDYYYVDMERYEIRGRHKKKSYRLGDK
                     VYVQIVDVDLVFYRVDLKLVENDKAAKP"
BASE COUNT          794 a          684 c          790 g          668 t
ORIGIN      
        1 gaattttaat ctcggtacca tccggaatta ccgaggtgga gaagcgcgct gtccgcgact
       61 cggccgaaaa tgctggggcc agggaggtgt atttgattca tgagcctatg gctgccgcta
      121 tcggtgtggg tttaccggtc gatcagccct ccggtatcat gataatagac atcggcggcg
      181 ggacctcgga aatagcggtg attgcacttt acggcattgt caataacagg tcgatccgta
      241 tcgccggcga cgaaataaat gaagctatcg taatatactt aaaaaagaat tataatctcc
      301 tgattggtga gctgactgca gaagagatca agatgaagat tggttcggca tttccaatgg
      361 aacgcgagga atcggttgaa ataaaagggc gcgatctggt ggcgggagta cccaaaaaca
      421 tgaaaatctc atcggcccag gtgagggagg cactgactga accgatcgag gccatcatag
      481 aagctgtgcg gctttcactg gaggccactc cccccgagct ggcatccgat atactcgacc
      541 gcggcattgt gctcaccggc ggcggtgccc tgctgcgcgg attcgccaag cgtatcaggc
      601 acgaaaccaa cctgcctgtt tacgttgccg atgatcccat gacatgtgtg gcccgaggca
      661 caggaaccgt gctcgagaat atgaatgctt tcagcaaggt tctgatcaag tcccgcaatg
      721 attgatcttc agtcgcgcct caaaaagccc tgaaataaat tgacatatct gtctgctgtt
      781 cttcatttca agcaaaggga ttgatatgga tattaccgct gagaaaatag tagcctacgt
      841 ttccaaagaa aattatcgtc cggtaagacc gcgcgagctt gccaaagaga tgaagattcc
      901 cgaaaaaaat taccgcaaat tccggaacct gttaaaaaat ctcatatccg acggggagct
      961 tgtaaaaatc cgcgggggaa gaattggccc gccggagaag atgaatctca aggtgggcaa
     1021 aatccagatc acctccaaag gcttcggctt cctgatgcct gatgacggca aagaggagat
     1081 ctacatacga gccaatgata ccaagactgc attgaacggc gatagagtcg ttgtcagggt
     1141 caaaccctac aggactcccg gcaagaagtc cgagggtgag gtggtgaagg tgctggaacg
     1201 ggccagaaat acgattgtcg gtacttacca ctcctccaag tattttgaat atatagaacc
     1261 cgatgatccc tctttcaagc gggatgttta cataatgccc gatcacggcc tctcgcccag
     1321 ggatggccag aaggttgctg tcatacttaa agaatggaaa aaccagttcc tcaatcccga
     1381 gggaaagctg gttgaagttc tcggatttcc gcatgaagcg ggtgtggatg tcttaagtgt
     1441 catcaagaag tttgagcttc cattggagtt ccaggctccg gtgctgaggg aagcctcaaa
     1501 agttaaaatt aacataaccc ctgccgagat aaaaaaacgc cgcgactatc gagatcagat
     1561 aaccttcaca atcgatccgg ttgatgccaa agatttcgat gatgccctct cgatacgaaa
     1621 aaacagcagt ggcaattacc ttgtaggggt gcatatagcc gatgtttctc actatgtcgg
     1681 cgagggcagc tccctggata aggaggcccg caaccgggga acttcggtct acctggtcga
     1741 tcgtgtgctg cccatgctcc cggagcatct ttccaacgaa atctgcagcc tgaagggagg
     1801 cgaggatcgt ctgacctaca cctgcgaaat ggagattaat gacaagggcg atgtcgtaaa
     1861 atttgagata ttcaaatcga ttattaatag ctcggccaga ctggattatg atcaggtcca
     1921 ggaatatttc gataccggcg gcacggacag tgttcccgat aatgtggcca gggcccttga
     1981 accattgcgc aagattgcca ggaaagttcg ccagcgccgt ttccagaagg gcagtctgga
     2041 ttttgatctg cccgagcctc tggtaatcat ggatcagagc ggggaggttg tggatattca
     2101 ggtacgaccc cgcaaagaga gccacaggct gatcgaggag ttcatgctgc tggccaataa
     2161 atgcgtggcc agacatttcg tgcgcctggg gcttccaacg ctcttccgcg tccatgacag
     2221 gccggacaag gataagattg aggcttttca ggaatttgca aaatcattcg ggtatgaatt
     2281 cagttttccc gatccggtca agcccaaaca tctggcggac agcatcagag aatttgaagg
     2341 aaccccggag gaggacctct tgaatgagat tctcctcagg tcgttgaaaa aagctcagta
     2401 ccaacgggag aatatcggcc atttcggatt ggccttcgag aactacctgc attttacctc
     2461 gcctatcagg cgttaccccg acttaatggt gcaccgtctt ttgtcggaga taaagaacaa
     2521 aaaatatccg ggcacgcgca cggcttctgt gaatgagttc ctggatcgag ttggcaagca
     2581 tagcaccgac atggaaataa ttgccgaaaa ggcggagcgt gagacaatca agatcaagca
     2641 ggtgctttac ctttcgcgcc agatcggcaa tatttaccag ggcataattt caggtatgac
     2701 ctcgggcgga ttttttgtcc ggctcatgca attttcggcc gagggcatgg tgcggttgtc
     2761 catgatggtg gatgattact actatgtcga catggaaaga tatgaaatcc gcggccgtca
     2821 taaaaagaaa agctaccggc tgggggacaa agtatatgtg cagattgtcg atgtcgatct
     2881 ggtgttttac cgggtcgatc tgaaattggt ggaaaacgac aaagcggcca aaccca
//
LOCUS       DSEB01000071            3795 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002423, whole genome shotgun sequence.
ACCESSION   DSEB01000071 DSEB01000000
VERSION     DSEB01000071.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002423"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..98)
                     /locus_tag="ENO07_01330"
     CDS             complement(<1..98)
                     /locus_tag="ENO07_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDS00636.1"
                     /translation="MPRILVVDDEENFRKLYSMELSSEGYDVSLADS"
     gene            complement(100..1848)
                     /locus_tag="ENO07_01335"
     CDS             complement(100..1848)
                     /locus_tag="ENO07_01335"
                     /inference="COORDINATES: protein motif:HMM:PF00512.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDS00637.1"
                     /translation="MPSLSKNVCQADSACDPSSSFIDNLPFPAFLCSEGLEIISINPA
                     MINLLGVNTRGDVRITSLCQVESILGDRLDPYPEQSSIRQGSHISFTNLRGEMRYCTV
                     SVMPVEVKTGKIFIYVLQDESRTYMLGERLARKNSELSIFSQVVSALGSILNFDEILQ
                     IILIAITAREGLGFNRAFLFLYNQDERQLEGYTGIGPSSPEEAGRIWSSLPDEGRSLM
                     EVLKTYSATMNDSAENVSNRLKNLKIPLSHEHHILGEVVFQKNPRIIGGAGCSCGSEC
                     GICTTLGVEQFAAVPMIAGKNVLGVITADNFITGRTIRHRDLSQLQVFANQAAIAIER
                     TRLYQSLSDYLKKLEKANVNLRNAQEELLKIERVTLWSELTSDIAHELRNPTSIIGGF
                     AALIIKSPDLPDNIREQARIIYDECTRLESALNTVLDFSKSFAQDKAEFDIYEVVCEI
                     KEIFLAKEVNRQIRVHKPDDAGELVAEGRVDQIKFAFYTIMSMISEKVDPACCINIRF
                     YSADNNVKAVFEIESSNFSETDYLAEIADPSAGRIALRMSMAFEAIKYNGGNLGIDSS
                     AEGRARIYVEWPNARR"
     gene            complement(1901..2554)
                     /locus_tag="ENO07_01340"
     CDS             complement(1901..2554)
                     /locus_tag="ENO07_01340"
                     /inference="COORDINATES: protein motif:HMM:PF07238.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HDS00638.1"
                     /translation="MCGSGRNSSRNARSTQYLVLETPPARSGFNLYSTSDHAQAERPD
                     IGGNMTRQNPVKGKATGKKPKPAVKKPFKVYSHKRRKYIRLEVNSPLKFKTFNPAEPD
                     LEIDPHQACEGSILNISGGGVLFETEYSVRENDYLVMELSLVDADRLCGIIGKVKRVD
                     TEEGTPLVGIEFMSPEKLRAELPTHVLDKIDEEIYSFDEHLRKALLKYVFSQQVENA"
     gene            complement(2578..3783)
                     /locus_tag="ENO07_01345"
     CDS             complement(2578..3783)
                     /locus_tag="ENO07_01345"
                     /inference="COORDINATES: protein motif:HMM:PF09986.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HDS00639.1"
                     /translation="MVKQQDSPIFMTGIECPVCGTEYEVETIKVGAYIEAGRDTDFCP
                     QERKWNNPDFQKYNPLLFFMAVCPDCFFCKEITLKFKEWKTDTAFKNDHLKNLKEKHL
                     RELQDDDGVIRKLALNIDADKYPFQTAVIKILLGIYDEKFNSNYSNLDVGRYFLRIAW
                     LFRENTQSHTHGDGQLASHAMRIEDIILRLSGYYEKLREEKQGLAEASVEFIKDPDFP
                     EGDRKRELSEIYNDALSDLDTAIEKYKSAVVKLKGSIHSSSEVIEASSESLAPLEQPF
                     NKYSSFREYLRELKAKWPEVPISEDEAMNSAANYYKAAYHGGKEISSGNQAIQVVYLI
                     AELSRRIGEHEEARGYFGTSIKLAREYIRANKGDRSRTALARRILELATEQGELNLKM
                     LEQRKHVPA"
BASE COUNT          882 a         1009 c          842 g         1062 t
ORIGIN      
        1 gaatcggcca ggctgacatc atagccctcg gagctgagtt ccatgctata gagctttcta
       61 aagttctcct cgtcgtcaac gactaatatc ctcggcatat catctccttg cattaggcca
      121 ttccacataa atgcgtgccc tcccctcggc acttgaatca attcccagat ttccgccgtt
      181 atacttaatg gcttcgaacg ccatactcat ccgcagtgcg atcctgccgg cgctgggatc
      241 tgcgatctcg gccagataat cggtttctga gaagttcgag gattctattt cgaatacagc
      301 ctttacatta ttatcggcag aataaaaccg tatgttaatg caacaggccg gatcgacttt
      361 ctccgatatc attgacatta tagtataaaa tgcgaatttt atctgatcga cacggccctc
      421 agcaacaagc tctccggcat catcgggctt atgtacccgg atttgacgat taacctcttt
      481 ggccagaaaa atttccttta tttcgcagac aacttcatat atatcgaatt cagctttgtc
      541 ctgggcgaat gattttgaaa aatccaaaac tgtattgagc gcgctttcaa gcctcgtgca
      601 ttcatcataa ataattcgag cctgctccct gatattatcc ggcaggtcag gtgatttaat
      661 tatcagcgcc gcaaagcccc ctattatcga ggtcggattc ctaagctcat gggctatatc
      721 ggaagtcagt tcgctccaga gcgttacccg ttcaatcttg agtaattcct cctgggcgtt
      781 gcggaggttt acgttggctt tttccagttt tttaaggtaa tccgaaaggc tctgatacag
      841 gcgggtcctt tcgatagcga tggccgcctg attggcgaaa acctgaagct gggacagatc
      901 ccgatgcctg atagtgcgtc cggttatgaa attgtccgcg gttatgacac ccagcacgtt
      961 cttgccggct atcatcggta cagcagcaaa ctgttcgaca cccagggtcg tacaaatccc
     1021 gcattcggag ccgcagctgc atccggcgcc tcctatgatc ctgggattct tctggaacac
     1081 cacctcgccc aggatatgat gctcgtgact caacgggatt ttcagattct tcagcctgtt
     1141 cgagacattt tcagcagaat cattcatggt ggccgagtat gttttcagga cctccatcag
     1201 gctccggccc tcatcgggaa gagaactcca tatccgtccc gcttcttcag gggacgaggg
     1261 cccgataccg gtgtaaccct ccagttgcct ctcatcctgg ttgtacagga ataaaaaagc
     1321 ccggttaaaa cccaagcctt ctcgagcagt tatcgcaatt agaattatct gaagaatttc
     1381 atcgaaattc agtattgaac ccagagccga caccacctgc gagaaaatcg agagttcgga
     1441 attcttgcgc gccaaccgct cccccagcat ataggtcctg ctctcatcct ggaggacata
     1501 gataaaaatc tttcccgtct tcacctccac cggcattacg gagacggtgc agtaacgcat
     1561 ctcaccccga agattggtga aacttatgtg gctgccctga cggatgctgc tctgttccgg
     1621 ataaggatcc aatcggtcac ccaggatgga ttcgacctgg cacagacttg ttatccgcac
     1681 atcaccgcgg gtattgactc ccagtagatt gatcatcgcg ggattaatag aaatgatctc
     1741 gagcccctcg ctgcacagga acgcgggaaa gggcagattg tctataaatg aacttgaagg
     1801 atcgcaagcg gaatcagcct gacacacgtt tttgctcaaa gatggcatgc ttcaattccc
     1861 ggttatattt ccagtttgga cggctcagag cgttctcatt ctatgcattt tccacctgct
     1921 gagagaaaac atatttcagg agcgcttttc gcagatgttc atcgaaactg tagatctcct
     1981 catcaatttt atccagcaca tgcgtgggga gctcggccct caatttttcc ggtgacatga
     2041 actcgattcc caccagtgga gtgccctcct ccgtatcgac tctcttgacc ttgccgataa
     2101 tcccgcaaag gcggtcagca tcgacaaggg ataactccat gacgaggtaa tcgttttcac
     2161 gtaccgaata ctcggtttca aatagaactc ctcccccgga gatgttgaga atgctcccct
     2221 cgcaggcttg atgcgggtct atttccaggt caggttcggc cggattaaag gtcttgaact
     2281 tcagcgggga gttcacctcc agcctgatgt attttcttct tttgtggcta taaaccttga
     2341 aaggtttttt cacagccggt tttggtttct taccggtagc ttttcctttg acaggatttt
     2401 gccgggtcat attacctcct atatcaggcc gttctgcctg agcgtgatct gaagttgaat
     2461 acaaattgaa tccactccgg gctggtggag tctctaaaac cagatattga gtcgagcgag
     2521 cattccggct cgagtttctc ccggaaccac acatttactt tctttgcgga tgtcgcttca
     2581 agcgggcaca tgttttctct gttccagcat ctttaagttc aattcaccct gttccgtcgc
     2641 aagctcgaga atcctgcgtg ccagagctgt tctggaacga tcccccttgt tggcacggat
     2701 atattcccgg gccagtttga tggacgtgcc gaaatagccg cgggcctcct catgctctcc
     2761 aattcgccgg gataattcgg cgatcagata gacgacctga atagcctggt tgccggagga
     2821 gatctccttc cccccatgat atgcagcctt ataatagttc gctgcggaat tcatggcctc
     2881 atcttcgctg atcggcacct ccggccattt cgccttcaac tcacgcaggt attcgcgaaa
     2941 gctgctatat ttgttaaaag gctgctccag gggagctaat gactccgagc tcgcctctat
     3001 cacttcactg cttgagtgta tgcttccctt taattttacc accgcggatt tatacttttc
     3061 aatcgcggta tccaggtcgg agagtgcatc attatagatt tctgacagtt ctcttttcct
     3121 gtctccttcc ggaaagtctg gatcttttat gaattctact gacgcttccg ccagcccctg
     3181 cttctcttcc cgcagcttct catagtatcc cgacaacctc aggataatat cctcgattct
     3241 catggcatgg ctggcaagct gtccgtcgcc atgggtatgg ctctgcgtat tttctctgaa
     3301 gagccaggcg attctgagaa aataacggcc gacatcaaga ttggaatagt tcgaattgaa
     3361 tttttcgtca tatatcccca gcaggatctt tattaccgca gtctggaagg gatacttgtc
     3421 ggcatcaata ttaagagcca gcttacgaat caccccgtca tcatcctgaa gttcccggag
     3481 gtgcttttcc ttgagattct tcaaatgatc attcttaaaa gcagtatcgg ttttccattc
     3541 tttaaatttc agagtgatct ctttgcagaa aaagcaatca gggcacacag ccatgaagaa
     3601 caggagcgga ttatatttct ggaaatcagg attattccat tttctttcct gcggacagaa
     3661 gtcggtatcc ctgcccgctt cgatataggc tccgaccttt attgtctcga cttcatactc
     3721 agtgccgcat accgggcatt caatcccggt catgaaaatc ggcgagtctt gctgttttac
     3781 catgatctcc tctac
//
LOCUS       DSEB01000072            2424 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1006631, whole genome shotgun sequence.
ACCESSION   DSEB01000072 DSEB01000000
VERSION     DSEB01000072.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1006631"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(406..1350)
                     /locus_tag="ENO07_01350"
     CDS             complement(406..1350)
                     /locus_tag="ENO07_01350"
                     /inference="COORDINATES: protein motif:HMM:PF04264.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HDS00640.1"
                     /translation="MYIHAFMKGEIMRKAFLLALFLMIVTGALLLAQEGEMREEQVIP
                     ETTTFYFGQGLENDVLMVRSLAIFDSRAPLGSVVGLVRGMTGELTLWLDSIKLVEVEE
                     PEPVMEEADQPDTMAEADTAMEMAAEEEAPAEEEPEEQMDTVRVTPPAPRAVPPSGYT
                     QPVMVMEIPLDRLTTGNPQLDEMLKSENYLNVSEFPTATFVLTNVTDPSEYRLQDMRE
                     ISLIGSGELTLNGLSKRYANIRVFMTYIEEKPITAENTGLTGDLIHITAEFSISLSDF
                     AISIEPENLLTIDDRVNIMFDGFGSTNRMSASAPAESE"
     gene            1717..2013
                     /locus_tag="ENO07_01355"
     CDS             1717..2013
                     /locus_tag="ENO07_01355"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HDS00641.1"
                     /translation="MVEEIEKLAAVFKALSDPTRLQILKILEAGDRSVTEIVDYFSLS
                     QPTISRHLAALKASGLIKSKKQEQQKIYSLDTEALKSFLREYFGSFENLKGYKL"
     gene            complement(2146..>2424)
                     /locus_tag="ENO07_01360"
     CDS             complement(2146..>2424)
                     /locus_tag="ENO07_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HDS00642.1"
                     /translation="VNKIGTYGVAVLAKHHNIPFYVAIPWSTYDENTPAGEDVEIEMR
                     DPEEVTNWSGVRTAPEGVNVYSPAFDVTPHELVTAIITDTGIVYPNKK"
BASE COUNT          620 a          623 c          525 g          656 t
ORIGIN      
        1 ggataagcca acaaaaacaa tgatatttca caacatgttt atttttatta cttaatagag
       61 atgtataaaa gaagaatgcg cgtaaagctt tgaaaaactg cgtttcccta gacgtcccta
      121 taataactcc ataccaaaaa ttgtcaagag ttttttagta atttctgcaa ttttttttat
      181 ttccggctcc ggggccatat ttgtcaaaaa ttttgagctt ttttttcatt tttcccaaaa
      241 agcgctttcg acttcgtcag ggcctgtcgt ttctcttgac ttgattccct aagaagcaaa
      301 ggctacctgc atttatgcgt atatgtgcat ttatgttgaa atgataaagc cgggctggat
      361 atgcccggct tgagtttgct gcgatccaaa gcttctgctg agccgctatt ccgattctgc
      421 cggcgccgag gcgctcatcc ggttcgtgga tccgaaacca tcaaacatga tattcactct
      481 gtcatcaatt gtgagaagat tctccggttc aatcgatatc gcaaaatcag aaagagagat
      541 agaaaattca gcggtaatat gtataaggtc gccggtcagg ccggtatttt cggctgtgat
      601 cggcttctct tctatatatg tcataaacac gcgaatgttg gcgtagcgct tggaaagccc
      661 gttcagggtc aattccccgc ttcctataag gctgatttca cgcatatcct ggagcctgta
      721 ttcggatgga tcggtaacgt tggtcaatac gaaagtcgca gtaggaaatt ccgatacatt
      781 tagataattc tcggatttga gcatctcgtc cagttgcgga ttcccggtcg tgagacgatc
      841 cagtggaatt tccataacca tcaccggctg ggtgtagccg ctgggcggga cagccctggg
      901 ggccggaggg gtgacacgaa ccgtgtccat ttgctcctcg ggttcctctt cggctggcgc
      961 ttcttcctcg gcagccattt ccatagcggt atcagcctca gccatcgtat cgggctgatc
     1021 ggcctcctcc attaccggct caggttcctc cacctcaacc agtttgatgg aatcgagcca
     1081 gagagtcaat tcccctgtca tacctctgac aagaccgacc accgaaccca atggcgccct
     1141 gctgtcgaaa atagccagcg acctcaccat aagcacgtca ttttcgagcc cctgcccgaa
     1201 atagaatgtc gttgtttctg gaattacctg ctcctcgcgc atctcaccct cctgggccag
     1261 aagcagggct ccggtcacaa tcatcaggaa taaggccaac aaaaacgcct ttctcattat
     1321 ttctcctttc atgaatgcat gtatgtacat ataataattt gctatctatc ggaagaattg
     1381 aatcaatcag ctctttttgt gtttcgcccg ggctatgtca gctcatgaat catcaatccg
     1441 caattcacct ttgtgccgac aggcccggaa atcaatctga aaaaccacca tccacacggc
     1501 cagtttgccg tagcctcaaa ataaatccct gcaatttatg caaaaagcta tcgtttgcca
     1561 agctaaatta tgcaaaaatt atttctcaaa tggtcgacat tcgaaggctc aatattgttc
     1621 attaaaaatt cttacgccgc atgaatgtta agtgttgact ttggtcctcc gataatatat
     1681 acataattgc gcatatgcgt ataacgttag gattaaatgg ttgaggaaat cgaaaaactt
     1741 gcggctgtat tcaaggccct ttccgatccg acccggctgc agattttaaa aatattggaa
     1801 gcgggcgaca gatccgtaac cgagatagtc gattatttca gcctctccca gccgacaatc
     1861 tcgcggcacc tggccgcgtt gaaagcctcc ggcctgatca agtcaaaaaa acaggaacag
     1921 caaaaaatct actctctgga taccgaggcc ttaaaatcat ttctgaggga atatttcggc
     1981 tcattcgaaa acctgaaagg ctataagctc taatcagttc gtgcagcgcg tacctcccgg
     2041 tacgccctgt ctgacacagc gcatgaggac atacgcctgc gctgcccttg acgggccgcg
     2101 cacgatcctt gcgcgtgcac ccgtaaacgc gcccctctat agcaatcatt tcttattcgg
     2161 atagacaatc ccggtatcgg ttataatcgc cgtaaccagc tcatgggggg tcacatcaaa
     2221 cgccggtgaa tatacattga caccctcagg ggcggttctg actccgctcc agttggtaac
     2281 ctcctccggg tcacgcatct cgatttcgac atcctcaccc gcaggcgtat tttcatcata
     2341 tgtcgaccat ggtatggcca cataaaatgg gatattgtga tgtttggcca gaaccgccac
     2401 gccgtatgtg ccgatcttgt ttac
//
LOCUS       DSEB01000073            2747 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004999, whole genome shotgun sequence.
ACCESSION   DSEB01000073 DSEB01000000
VERSION     DSEB01000073.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004999"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..289)
                     /locus_tag="ENO07_01365"
     CDS             complement(<1..289)
                     /locus_tag="ENO07_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00643.1"
                     /translation="METHVVKVDNPKELNLIFGMTHFIKSVEDIYEIMVCAVPNIKFG
                     MAFCEASQDRLIRTTGTDDGLVKLAADNMLKIKCGHTFLIFLGNVFPINVLN"
     gene            complement(327..1574)
                     /locus_tag="ENO07_01370"
     CDS             complement(327..1574)
                     /locus_tag="ENO07_01370"
                     /inference="COORDINATES: protein motif:HMM:PF13646.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00644.1"
                     /translation="MDCKASILRAIMEVGDKKIIKAMMNLLSQREHPLVTAMALSLIG
                     KFQMSALSSKVEPFLKNEERFLRGEALIALARLNRTKFMEIIPDEIENYNWYLRMKTA
                     EALKFIETPEGANLLEKLQSDADHRVRTAALYSLAEYDFVEMEDYLGTALEDSDPAVV
                     SSVIELIAKLKLHQFSKKTLDIYEDEMDEADAGFRFSILYNLSGWVVDSQITDENILE
                     LLNKALDDRDRNVRQEAIDIFAMIGMDKSEYLGFFEPMVTASTYDLFYGRFDENPVAE
                     INTERGQIRVRLLYNMAPKTVSNFIKLAESGFYDGVIFHRVVPNFVIQAGDPHGDGFG
                     GPGYTIRSEFNRHEYTRGTMGMAHAGKDTGGSQWFICHSPQPHLNGRYTAFGQVIYGM
                     RIVDRILVGDKINSIEIIYPGEE"
     gene            complement(1514..2308)
                     /locus_tag="ENO07_01375"
     CDS             complement(1514..2308)
                     /locus_tag="ENO07_01375"
                     /inference="COORDINATES: protein motif:HMM:PF13646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HDS00645.1"
                     /translation="MNRVFVNLFLIVCIAAITGCGGEEEKAALPLDKNVQIAIWEDMR
                     QADTLLYAYLDDDDPGIRLRACYALGMIEPEGASAYIADLLDDPEKDVRIEAIFVMGL
                     IGDSMYAERLIPLLFDPDSQIVHQAAIALGRMGGDAAVRALMSSARDTIPEHRIWAAE
                     GLWRADADTAVELLEKLAADSSVSVEQAAIYALRRLEARSAAEILRARIKDTIPEIRM
                     YAAEGLRAIKDSASLMALSNQLSREREWTVRPVFCGRSWKLAIRRL"
     gene            2473..>2747
                     /locus_tag="ENO07_01380"
     CDS             2473..>2747
                     /locus_tag="ENO07_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00646.1"
                     /translation="MSISVFLLEQYDDEFVDKLRKKLDAEIDLYYGEEIPDPAEYEIL
                     VAGVPEREHLTASPRLHTLIIPWAGIPTRTRELLLEIPDIKVHNLHY"
BASE COUNT          697 a          730 c          601 g          719 t
ORIGIN      
        1 ggttgagtac attaatggga aagacgttac ccaggaaaat caggaatgta tgcccgcatt
       61 tgatcttcag catattatcg gccgccagct tgacaagacc gtcatcggtt ccggtggtcc
      121 tgatcaaacg gtcctgggaa gcttcgcaga aagccatacc aaacttgata tttggaacgg
      181 cacagaccat tatctcataa atgtcttcga ccgacttgat gaaatgggtc atgccgaaaa
      241 tcagattcaa ttctttcgga ttgtccactt tgacaacatg ggtttccatt gcagactcct
      301 tttatttgat acgataaatt accggtttat tcctctccgg gatagataat ttctatagaa
      361 tttattttat ccccgacaag gattcgatcc acgattctca taccgtaaat aacctgcccg
      421 aatgctgtat agcgcccatt tagatgaggc tggggggaat ggcagataaa ccactggctg
      481 ccgccggtat ccttgcccgc atgcgccata cccatggtgc cgcgagtgta ttcatggcgg
      541 ttgaattcgc tgcgaatcgt atagcccggc cccccgaatc cgtcgccatg cgggtcgccg
      601 gcctgaatca cgaaattggg aaccaccctg tggaaaatga caccgtcata aaaacccgat
      661 tccgccagct tgataaaatt cgagacggtt ttcggggcca tattataaag aagccgcacc
      721 cgtatctgtc cccgctcggt attgatttca gcaaccgggt tttcatcaaa gcgcccataa
      781 aacagatcat aggtagaggc tgtcaccata ggctcaaaaa atcccagata ttccgattta
      841 tccataccga tcattgcgaa aatatcaatg gcctcttggc gcacattgcg gtcacgatca
      901 tcaagagctt tatttagaag ttcgagaata ttttcatcgg taatctggct gtccactacc
      961 catccggata aattatataa aatcgaaaag cggaagccgg catctgcctc atccatctca
     1021 tcttcataaa tatcgagtgt ctttttcgag aattgatgca acttcaactt tgcaatgagc
     1081 tcgattacag atgataccac cgccggatcg gaatcttcca gcgctgtacc caggtaatcc
     1141 tccatctcca caaaatcata ttcagccaga ctgtaaagcg cggctgtgcg gacacgatga
     1201 tcggcatcgc tctgaagctt ttccagaaga ttcgccccct ccggggtctc gataaacttc
     1261 agcgcctcag cggttttcat acgcagatac cagttatagt tttcaatttc atcgggtata
     1321 atctccataa atttagtgcg atttaatctg gccagtgcaa taagggcttc ccctctcaga
     1381 aatctttcct cgtttttcaa aaatggttca actttagaag agagagcaga catctgaaat
     1441 tttcctatca ggctcagggc catggcggtg acgaggggat gttccctctg gctcaggaga
     1501 ttcatcatcg cctttataat cttcttatcg ccaacttcca tgatcgcccg cagaatactg
     1561 gccttacagt ccattcacgt tccctcgaaa gctggttgct gagcgccatc agcgaggctg
     1621 aatcctttat cgcccgcagg ccctcagcag catacatgcg aatctccgga attgtgtcct
     1681 ttatgcgcgc acgcagaatt tccgccgccg accgggcctc aagccttctc agtgcataaa
     1741 tcgccgcctg ctcaacagat accgagctgt cggccgccag cttctcaagc aattcaaccg
     1801 cggtgtcggc atccgcacgc cagaggccct ccgccgccca gattctatgt tccgggatcg
     1861 tgtcccgggc tgaactcatc aatgctctta cggccgcatc accgcccatg cgtcccagcg
     1921 cgattgccgc ctgatgcaca atctgtgagt caggatcgaa taggagaggg atcaatcttt
     1981 ccgcatacat actgtcgccg atcagcccca ttacaaaaat tgcctcgatt ctgacatctt
     2041 tctcggggtc atccaggaga tcggcgatat aagccgaggc cccctccggc tcgatcattc
     2101 ccagagcata gcaggcgcgc aggcggattc ccggatcatc gtcgtccaga tacgcataca
     2161 gaagggtatc tgcctggcgc atgtcttccc agatagcaat ctgcacattt ttatccaacg
     2221 gcagtgctgc tttttcttct tctccaccgc atccggtgat tgcagcaata catacgatta
     2281 aaaataaatt tacaaatact ctgttcattg aactcccctt acctgttttc aagtaaatta
     2341 atcgggctct ggcggatttc aagtaataaa taatttttta aatgatttta tgaatttcgg
     2401 ctcttcttaa aatcgttctt gccctgagcg ggattatact gttaattcag ttcaaataag
     2461 gaggcccttt ttatgtcgat atctgtattt ttgctggagc agtatgatga tgaatttgta
     2521 gataagttgc gaaaaaagct ggatgcggaa attgacctgt actacgggga ggaaatcccg
     2581 gatccggccg agtatgagat tctcgtggca ggagttccgg agagggagca tctcactgcc
     2641 agccccaggc tccataccct gatcattccc tgggcaggaa ttcctacccg cacacgcgaa
     2701 ctgctcctgg aaatacccga catcaaggta cataacttgc attacaa
//
LOCUS       DSEB01000074            2214 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1008076, whole genome shotgun sequence.
ACCESSION   DSEB01000074 DSEB01000000
VERSION     DSEB01000074.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1008076"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..2019
                     /locus_tag="ENO07_01385"
     CDS             <1..2019
                     /locus_tag="ENO07_01385"
                     /inference="COORDINATES: protein
                     motif:HMM:PF08448.8,HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDS00647.1"
                     /translation="VLDTVGAAIIVTDDTMRVEMYNEQAKKLLLGDEHRVIGDIFFNR
                     IKNCPYKTNQNELKKLIEQGQRKTHSEFVIEDDYYDVTVTPIADQDGHASKIIFMFFN
                     VTRQREEYNRQRLSAVVFESTSEGVVITDKDNRIVEINDALTHITGYQKEELLGRDPR
                     IFQSGIHNIEFYAELWDSLEKRGVWRGEIWDRRKNGEVFPAWQTINTICNEAGDVVNY
                     VSIFSDISAIKQSQEKLDYLAYHDPLTTLPNRVLFTDRLMQALEKSRRENNRLAIIFI
                     DLDRFKNINDTLGHAIGDQLLVDVGLRLLKNVRKVDTVARLGGDEFVVLLEDIESEEH
                     VAYFAEKLNRAFVEPFIVSDNQLHLTLSMGICISPADGTDVETLLKNADAAMYRAKDQ
                     GRNGFQFFTMDLSLRAHERLTLETNLRDAIKKEQLELYYQPQNLIEDGRILSAEALLR
                     WQHPELGRIPPENFIPVAEETGLIVSIGEWVITEACRQLRLWRDAGYAIPQIAINVSG
                     IQLQRGDLVSYVLKSCEFYQLSLSDLELEITESILMDDAERSIRILTQLQEYGASITI
                     DDFGTGYSSLGYLKRLPVNKLKIDRSFIRDIVNNADDAAIVRAILAMAENLQLIVIAE
                     GVEEEAHCRLLREQNCKIAQGHYFSRPVPAREFQELLDRELANRRLDS"
BASE COUNT          557 a          515 c          591 g          551 t
ORIGIN      
        1 gtactggata ccgtgggtgc cgctattatc gtaaccgatg acaccatgcg ggtcgagatg
       61 tacaacgaac aggcgaagaa gctgctgctg ggcgatgagc accgggtcat tggcgatatt
      121 ttctttaacc ggatcaaaaa ctgtccctac aaaaccaatc agaacgagct gaaaaagctg
      181 atcgaacagg ggcagcgtaa gacccactcc gaatttgtta tcgaggatga ctattacgat
      241 gtaacggtca cccccatcgc cgatcaggat gggcatgcca gtaaaatcat tttcatgttt
      301 ttcaatgtca cccgtcagcg tgaggaatac aaccggcaaa ggctttccgc cgtggtgttc
      361 gagagcacca gtgaaggcgt cgtgatcacg gacaaggata accgcatcgt tgaaatcaat
      421 gacgccctta cccatatcac aggttatcaa aaagaggaat tattgggccg tgacccccgg
      481 atattccaat ccggtattca caacatagag ttttatgccg agctttggga cagtcttgaa
      541 aaaagaggtg tatggcgggg tgagatctgg gatcggcgta agaacgggga ggttttcccc
      601 gcctggcaga cgattaatac catctgtaac gaggcaggag atgttgtcaa ttatgtctcg
      661 attttttccg atatcagcgc cattaaacaa tcgcaggaga aactcgatta tctggcctac
      721 catgatcctc tgaccacgct gcccaatcgg gtgctgttca cggatcgcct catgcaggca
      781 ctggaaaagt cccgacgtga gaataaccgt ttggcgatca tttttatcga tctggatcga
      841 ttcaagaaca tcaacgacac tctgggtcat gcaattggtg accaattgct ggtagatgtc
      901 gggttgcgcc tgctgaaaaa tgtgcgcaaa gtcgatactg tcgcgcgatt gggtggtgat
      961 gagtttgttg tgctgctgga agatatcgag tccgaagaac atgtagccta ttttgccgag
     1021 aaacttaaca gggcttttgt cgagccattt atcgtcagtg ataatcagtt gcatctgact
     1081 ctgagtatgg ggatttgtat cagccccgcc gatggcacgg atgtcgaaac cctgcttaaa
     1141 aacgccgacg cggccatgta ccgtgccaag gatcaggggc gtaacgggtt tcagtttttc
     1201 accatggatc tttcgctcag ggcccatgaa aggttgacgc tggaaaccaa tctgcgcgat
     1261 gccatcaaaa aagaacaact tgaactctat tatcagccgc agaatctgat tgaagacggg
     1321 agaattctct cggcggaggc gttactgcgc tggcaacatc ccgaactggg cagaattcct
     1381 cctgaaaact tcattcctgt tgctgaagaa accggcttga ttgtttcgat tggagaatgg
     1441 gtgatcaccg aggcctgccg acaattacgc ctttggcgtg acgcagggta tgctattccg
     1501 caaattgcga tcaatgtatc gggtatccag ttacagcgtg gtgatctggt gagttatgtg
     1561 ctcaagagct gtgaatttta tcagttatct ttatcggatc tggagctgga gattaccgag
     1621 tccattctta tggacgatgc cgaacggagt atacgtattc tgacccagtt gcaggaatat
     1681 ggcgcctcga tcacgattga tgattttggt accggctatt catcactcgg ctatctcaag
     1741 cgtcttcctg ttaataaact caaaattgac agaagcttta tccgcgatat tgtcaacaat
     1801 gccgatgatg cggctatcgt cagggcgatt ctggccatgg cggaaaatct tcaacttatt
     1861 gttattgccg agggcgtgga agaagaggcc cattgccgac tgttacgtga gcaaaactgc
     1921 aagatcgccc agggccacta tttcagtcga cccgtccctg ccagggaatt tcaggaactg
     1981 ctggatcggg agctggcgaa tcgccggttg gattcttaat ctcactttcc cgacaaaaga
     2041 aaaagccacc gcaagggtgg ctttttcttt tgtcggtggc ggtgtcgtag cgatgcgcag
     2101 gtatgccgat gaagctccgg aggagattcc gggtgcggcg ggaatcggca cctgggaacc
     2161 tcctggcgga gtttcatcaa gtgtacctgc gctttgctac ggcacacata gaac
//
LOCUS       DSEB01000075            3250 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003459, whole genome shotgun sequence.
ACCESSION   DSEB01000075 DSEB01000000
VERSION     DSEB01000075.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003459"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..380)
                     /locus_tag="ENO07_01390"
     CDS             complement(<1..380)
                     /locus_tag="ENO07_01390"
                     /inference="COORDINATES: protein motif:HMM:PF00724.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HDS00648.1"
                     /translation="MDIFAPLKIGSVEIPNRLIMAPVKTGYGTLDGEVTYLHQAYYRR
                     RAEGEVGGIIVEPLYVDIAGKEHPRQLGISSFNHIRGLAQLVDTIHKNGVIAIAHLNH
                     AGRVANPEVSGMMPEAPSEIACFN"
     gene            complement(542..733)
                     /locus_tag="ENO07_01395"
     CDS             complement(542..733)
                     /locus_tag="ENO07_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00649.1"
                     /translation="MQEEESKKPALGHGIYHPGGRPMKVFRDAEGCLWLCDKGIDPNK
                     EFAQQGCWRCRDLAFTRND"
     gene            complement(1092..2447)
                     /locus_tag="ENO07_01400"
     CDS             complement(1092..2447)
                     /locus_tag="ENO07_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDS00650.1"
                     /translation="MNNMNQKNLSCEELLIIIDSISDGVFAVDTELKITFLNKAAERI
                     TGYTAEEAMGRPCKEIFRSNICESHCALKETMATKKPVVNRPVCMTNKAGRRVPISIS
                     SALLIDLEGRIIGGVETFRDLDLVEKLRKDFAARYQMEDIISRDKKMLEMFKILPTIA
                     ESDSTVLIEGETGTGKELVARALHSLSSRKKEAFVDINCGALPDTLLESELFGYVAGA
                     FTDAKKSKKGRFALAEKGTLFLDEIADISAAMQVKLLRVLQEKSYMPLGATKAKQTDV
                     RIIAASNRELESLVANDEFRRDLYYRINVVKLVIPPLRERKGDIPLLADHFIGCFNRL
                     RKKDIAGITPPALNLIMKHDFPGNVRELENIIEHAFVISPGGVIRPEHLPEYLHGRQA
                     IPAIEIAASMEEMESLFILSALKRNNWSRKDTARELGIHTSTLYRKIRKLKLKVPSSE
                     S"
     gene            2953..>3250
                     /locus_tag="ENO07_01405"
     CDS             2953..>3250
                     /locus_tag="ENO07_01405"
                     /inference="COORDINATES: protein motif:HMM:PF09941.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2173 family protein"
                     /protein_id="HDS00651.1"
                     /translation="MIGLDRLMKIKGVVAAGQFSEDGKVLRQVGELPEDLMESAELCV
                     NQNHTCHDFLSSLNGRSSRDYGSLVGWTVWGSKYSVVTVGNTRVFIETQRGDYNQ"
BASE COUNT          804 a          813 c          718 g          915 t
ORIGIN      
        1 ctattaaagc aggcaatttc cgagggcgct tccggcatca tccccgagac ttcagggttt
       61 gcaacacgcc cggcatgatt taaatgggcg atggctataa cgccgttctt atgaattgta
      121 tcaacaagct gtgccaatcc tctgatgtga ttaaagctgg agatgccaag ctgcctcgga
      181 tgttctttgc cggcgatgtc aacatacaat ggctcgacaa ttatcccgcc gacctctcct
      241 tccgcccgac gacgataata ggcctgatga agatatgtca cctccccgtc cagcgtgcca
      301 taacctgtct taaccggggc cattataagc ctgttaggta tctctaccga gccaatcttt
      361 agaggcgcaa atatatccat tcctctcccg ctgttgacga tgaatgccgg gacaatcact
      421 gcgaatagaa tatctcatcc tacctgctgc ttgattccac ggtccttttc accctcatgt
      481 ccatgctgtt ttcgattcca atcgagactg aaacatcccc tcacacaccc gcaaatctgc
      541 atcaatcatt gcgtgtaaat gccaggtccc tgcatctcca gcatccctgc tgcgcaaact
      601 ctttatttgg gtcaataccc ttatcgcaaa gccataaaca gccttcggca tcgcgaaaaa
      661 ccttcatcgg ccgtcccccg ggatgataaa tcccatgacc aagggccggt tttttgcttt
      721 cttcctcttg catcctaatc acctccaaat gttaattttt ttaatggagc caaacaaatt
      781 gataactgct gcggagtcga tcacttgaag tataccggat ggcattttca gagctcgatt
      841 caattaatat ttggtccagt ttaaatctcc attttatctg ttttcttcaa tataagaata
      901 agcaagcgac atgccggatg cggcccatgt taataaatca atcttatccc gttataaatg
      961 aatgtgttgc aagggcggcc gtaccgcttt tgcgggatcg tccggggacc ggatagatta
     1021 accgtgatgc aaaaatgcat tatattctat tcagatatca tgcacgttcg cacgtcatct
     1081 acgttttttg attaactttc tgaagatggt acttttagtt ttagttttct gattttgcgg
     1141 tagagtgtgg aggtgtgaat gccgagttcc ctggctgtat ccttgcggct ccagttattt
     1201 cttttgagag cgctcaaaat gaaaagagat tccatttctt ccatcgaagc ggctatctca
     1261 attgcaggta tagcctgtcg accgtgaagg tactcgggca gatgttcggg tctgatgacc
     1321 cctccgggac taattacaaa tgcatgttca attatgtttt caagctcccg tacattgccc
     1381 gggaagtcat gtttcataat taaattcagc gcaggcggtg tgatcccggc gatgtctttt
     1441 ttccgtaatc gattgaagca gccgatgaag tgatccgcca gcagggggat atcgcctttt
     1501 ctctcgcgca aagggggaat aaccagcttg acgacattta tgcgataata cagatctctg
     1561 cgaaattcat catttgcaac caaactttcg agttccctgt tagaagcggc aataatcctg
     1621 acatcggttt gttttgcttt ggtagctccc agcggcatat aagacttctc ctgaagcacc
     1681 cgcaacagct ttacctgcat ggcagcagaa atatctgcga tttcgtccag aaacagagtg
     1741 cccttttcgg ccagggcaaa tcgtcccttc ttggattttt tggcatctgt aaatgccccc
     1801 gcaacatacc cgaacagctc cgattcgagc agggtgtccg gtaacgctcc gcaattgata
     1861 tcaacaaagg cctccttttt cctggagctg aggctgtgca gcgcccgggc aaccagctcc
     1921 ttgcccgtgc cggtttcccc ctcaatcaaa accgtggaat cgctttctgc tatcgtggga
     1981 agaatcttga acatctcaag catctttttg tcgcggctga ttatgtcctc catttgatat
     2041 ctcgccgcaa aatcttttcg cagtttttcc accagatcaa gatcgcggaa agtctcaact
     2101 ccgccgatta tccggccttc cagatctatc agcaatgcgg aggatatact gatcggcaca
     2161 cgccgcccag ccttattcgt catgcatacc ggccggttga ccacaggctt cttcgttgcc
     2221 atggtctctt ttagagcgca atggctttcg caaatattgg agcgaaaaat ttccttacat
     2281 ggccttccca tcgcctcctc ggcagtatag ccggtaatcc tctcggccgc cttgttcaaa
     2341 aatgtaatct tcagctcggt atctacggca aagacaccgt cagagatgct gtctataata
     2401 atcagcagtt cctcgcagct cagatttttt tgattcatgt tgttcatcag gttttcgctt
     2461 tcgggccccg gcacaattaa ttctcgtact aacatataac tgaatttgac cgctaaatta
     2521 attataatgt aaaaaatacc ggaattcaac aataatgccg gccctggatg taatgattat
     2581 tgcgctcaaa aagttatgcc ggcggcaggg taaaatcaga tgggcacggg cagaaccaaa
     2641 gtctgacaaa gaaacatctt ttgtacggga catactcgag cattccgtgc tcatgcccgg
     2701 gttctgtgct tttcatgatc tcagaagaga tgtcccagcc gtttaagcga attccgggct
     2761 tgtcgacaat gcaacattgc atgatataac ttgtctgctg ttactgcgcc atctccttcc
     2821 ggcgaaaaat aatctcttgt aactgcttaa acaaaagcga gtttcgttat atgccttccg
     2881 tgattccggc ttttggcata gattctgcga ttggttatat ggggaaataa aaagattgcc
     2941 gaggaggtca agatgatagg tcttgataga ttaatgaaaa taaagggagt tgtcgctgct
     3001 ggacaatttt ctgaagatgg caaggttctt cggcaggtgg gagaattgcc ggaagatttg
     3061 atggaaagcg ctgagctttg tgtcaatcag aaccatacct gccacgattt cttaagttct
     3121 ctaaacggcc ggtcatcgcg ggactatgga tccctggtcg gctggactgt ctggggaagc
     3181 aaatattcgg tagttacagt cggaaatacc cgcgtcttta tcgagaccca gcgcggagat
     3241 tacaaccagc
//
LOCUS       DSEB01000076            3292 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003350, whole genome shotgun sequence.
ACCESSION   DSEB01000076 DSEB01000000
VERSION     DSEB01000076.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003350"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..393
                     /locus_tag="ENO07_01410"
     CDS             <1..393
                     /locus_tag="ENO07_01410"
                     /inference="COORDINATES: protein motif:HMM:PF00596.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDS00652.1"
                     /translation="IYNNDHDAMAVVHAHPPVTTAFSVAGKHFHVDILPEGIIVLDNI
                     AWIEYATPSSDDLPNLMEAHLPGSDIFVLKQHGVTASGKTLEEAYLKIETCEHQALIY
                     FYASLLGKPNRLKPEEIEKIRTIFSKKK"
     gene            complement(757..2076)
                     /locus_tag="ENO07_01415"
     CDS             complement(757..2076)
                     /locus_tag="ENO07_01415"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00512.23,HMM:PF02518.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HDS00653.1"
                     /translation="MNKDILPGDSRRERAIHELSALNEIATAINVSMSVEQISNTIVS
                     KAARQVDAEQGAIFLLSDERTVEAEFKTFIRKRESSRPGVPIHLNNMIKGWMILNKKT
                     LLCNDPENDDRFSPESFKDSGVSSLLATPLLERGHLMGLLVMFNKKAPGGFTDDNGRF
                     MYILGTQVAKVIENARLFERETRLLQRVHHQRMDSLARLVAGVAHELNNPIGAINSSM
                     ATLGNICRDFQGLMEDRVDGRGAMSEEITKRLAALDNIVSVIKSGGDRVAGIVNKLKN
                     FASLDQPELMTININSSIDDCLGILGPKYEGRIDIIRDYGDVPDIRCYASQVNQVLFH
                     VILNALESIPQKGRVEIKTGKSDGDLEIVISDSGIGILEKNLEKIFTPGFTTKGVGVG
                     VGLGLSICALIIEKHNGRIEVRSELGKGTSVSLRIPYSIRNGQNTGS"
     gene            2309..>3292
                     /locus_tag="ENO07_01420"
     CDS             2309..>3292
                     /locus_tag="ENO07_01420"
                     /inference="COORDINATES: protein motif:HMM:PF00155.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDS00654.1"
                     /translation="MKKKVIVEPSETLFKLPEPLGEQFKGLSRRVEKRNIEIIDLGKI
                     SIDFPDKLKSLLRIDQSEIWGDASRLADFELRLKQKIADWIKNDYDISLDPEHEMLLT
                     TGNTPGSFMAIMSLLDKNSRIFIPEPGYSLYRACALTAGAEIETYTVSEITDFQPNLE
                     KIRSRMGQNTKAIVVNYPHNPTSKGVDLKTYDRLSEFARKNNMIVIADSVYLLHGGDG
                     FSHPMYLQSNHGKASGIELITFSFLFNLPAFKIGVALGHRDFISPLRKFRATFNQIPS
                     VFDMEIADKLLDNREEVQKFFSQRFAENRKLVYDWLDKLGWEYLSSSYAPFV"
BASE COUNT          785 a          877 c          763 g          867 t
ORIGIN      
        1 atatataata atgatcacga tgccatggcc gttgttcatg cacatccccc cgtgaccaca
       61 gcattttctg tggccggcaa gcatttccat gtggatattc tgcccgaggg aattattgtg
      121 ctggataata tcgcctggat cgaatatgcc acgccctcga gtgatgattt gcccaacctg
      181 atggaggcac atctgcccgg ctcggacatt tttgtgctta aacagcatgg agttaccgcc
      241 agcggcaaga cacttgagga ggcctatctg aaaatcgaaa cctgcgaaca tcaggcgctg
      301 atatatttct atgccagcct cctgggcaag ccgaatcgcc tcaagccgga ggaaatcgag
      361 aagattcgaa cgattttcag caagaaaaaa taggaccctg ggtaccccac agcgcagctg
      421 tgggattatt taaattacag ccgaaaacta acatagcagt acagtggcaa tttccttcag
      481 gaattgacct acgaaactca ggaccccgcg atacggtaac ttcaggacgt ctgaattatt
      541 gctcagttat tccctttttt ttgttgcttt tttccctttt ctgttttcgc ttttccttga
      601 aggtcttttc agcctttttc cggttttctc ttccaccttt gtcctttttt cctttgtcac
      661 ccatatctac tccttgccca tttaatttcg ctaagaatat atcatgactt tatagcaaat
      721 gctcccgcag ctgaaccatg gcgcacccat tcaattttac gatcccgtgt tttgtccgtt
      781 cctgattgaa tatgggattc tgagcgagac cgaggtgccc ttgcccagct cgcttctgac
      841 ctctattctg ccgttgtgct tttcaattat cagggcgcag atcgacagcc ccagtccgac
      901 acccaccccc acacccttgg tggtgaagcc gggtgtgaag atcttttcaa gattcttttc
      961 cagtatgccg atgccggaat ccgagataac aatctccagg tctccgtcgg atttgcccgt
     1021 tttgatctca acccggcctt tctgcggtat cgactccagg gcgttcagga tgacatgaaa
     1081 gagcacctgg ttgacctgcg aggcgtagca tctaatatcc gggacatcgc cgtaatccct
     1141 gatgatatct attctgccct cgtacttcgg tccgagaata cccaggcagt catctatgct
     1201 gctgttgata ttgattgtca tcaattccgg ctgatccagg ctggcaaaat ttttcagctt
     1261 gttgacgatg cccgccaccc tgtccccgcc tgattttata acacttacga tattgtccag
     1321 ggcggcaagc cgtttcgtta tttcttcact catcgcacct cgcccatcca ctctatcctc
     1381 catgagcccc tgaaaatccc ggcagatatt tcccagagtg gccatcgagc tgttgattgc
     1441 gccgatcgga ttattgagct catgcgccac cccggcgaca agccgcgcca gcgagtccat
     1501 cctctggtga tgaacgcgtt gcaaaagcct ggtttcgcgt tcaaacagcc tggcattctc
     1561 gatcactttg gcaacctgag tcccgagtat atacataaag cggccgttat catcggtaaa
     1621 tccgcccggg gccttcttgt tgaacatcac cagcagaccc atcagatgac cgcgttccag
     1681 aagcggcgtg gccagcagtg aactcactcc tgaatccttg aacgattcag ggctgaagcg
     1741 gtcgtcattt tcagggtcgt tgcagagcag cgtcttttta ttgagaatca tccagccttt
     1801 tatcatgttg ttgagatgaa tcggcacacc cggccggctg gactctcttt ttctgataaa
     1861 ggttttgaat tcggcctcga ccgttctctc gtcgctcaaa agaaagatgg ccccctgctc
     1921 ggcatcaacc tgccgtgcag ccttgctgac aatagtattc gaaatctgct ccaccgacat
     1981 ggagacattg attgccgtgg caatctcgtt cagggccgac agctcgtgaa tcgctctttc
     2041 ccgcctggaa tcaccgggca aaatatcttt attcatgatg tgtgcccccg tttaagattt
     2101 ttttcagaga aaatgatcgc ttacccttta caaaattaag atacgcccgg cacagccgga
     2161 agtcaatatt attagaggaa atgcgaaata tttataatta ccctgtgccg catgagaaat
     2221 tgaaatgcgt gatctctgcc aaaataaatc cccaacaggg caggtatatt ttgacaaatc
     2281 cgaatcatga tatgtatatt aggggcttat gaagaagaaa gtcattgtgg aaccgtcaga
     2341 gacgctgttc aaattgccgg agcccctggg cgagcagttc aaagggctgt cccggcgggt
     2401 cgagaagcgc aatatcgaga taatcgacct cggaaaaatc agcattgatt ttcccgataa
     2461 gctgaagagc ctcttgcgga tcgatcaatc cgagatttgg ggggatgcct cccgtctggc
     2521 tgatttcgag ctgcgcctga aacagaagat cgccgactgg atcaagaacg actacgatat
     2581 ctcgctcgac ccggaacatg aaatgctgct gaccaccggc aataccccgg gatcattcat
     2641 ggccatcatg agcctgctgg ataagaactc gcggatattc ataccggaac cgggatactc
     2701 gctctatcgc gcctgcgctt tgacagcggg agcagagatt gagacctaca ctgtttccga
     2761 gataaccgac ttccagccca acctggagaa aataaggtca aggatgggcc agaatacaaa
     2821 ggcgatagtg gtaaactatc cgcataaccc gacctcgaag ggtgtcgacc tgaagacgta
     2881 cgatcgattg tcggagttcg cccgcaaaaa taatatgatc gtgattgccg attcagtcta
     2941 tcttctgcat ggaggggatg gtttcagcca tcccatgtac ttgcagtcca atcatggcaa
     3001 ggcttcgggc atcgagttga taactttctc atttctgttc aatctgccgg ccttcaagat
     3061 cggtgtggcg ctgggacatc gcgatttcat ctcccccctg cgcaagttcc gcgcgacctt
     3121 caaccagata ccatcggtct tcgacatgga aattgccgac aagcttctgg acaaccgtga
     3181 ggaggtgcaa aaatttttct ctcaaagatt tgccgaaaac cgcaagctgg tttatgattg
     3241 gctggataag ctgggctggg aatacctgtc ctccagctat gcgccttttg tc
//
LOCUS       DSEB01000077            8540 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000427, whole genome shotgun sequence.
ACCESSION   DSEB01000077 DSEB01000000
VERSION     DSEB01000077.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8540)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8540)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8540
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000427"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            734..1084
                     /locus_tag="ENO07_01425"
     CDS             734..1084
                     /locus_tag="ENO07_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00655.1"
                     /translation="MSDYHEPAEEISAHDRNIIRALKSLREEIEAVDWYHQRVAVCKD
                     GHLKAILAHNRDEEIEHAMMTLEWLRRNMDGWDEEMKTYLFTEGDITELEEHEEQSDE
                     GEKSSDLGIGSQKS"
     gene            1125..1928
                     /locus_tag="ENO07_01430"
     CDS             1125..1928
                     /locus_tag="ENO07_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HDS00656.1"
                     /translation="MDILKRSLAPITDGAWGEIEDVAKDVLKTHLSARKIVDIEGPKG
                     LDFAAVSLGRMDIGKGENKKEVGYGIRKVQPLIESRIIFDLELWELDNIERGADGIEL
                     DNLEDAARKIAQFEEDAVYHGFKDGNIQGIKGSSPYEKLKFSGDPHEYLALISKGITE
                     FVRASVDGPYALVVGPEMWNYIHSQTKGYPLKRQLENLLGGKIILNAFLKECYLFSVR
                     GGDMKLYLGQDLSIGYHAHDTEKVQLYFTESFTFRIIDPAVIMEIDWDK"
     gene            complement(1978..3204)
                     /locus_tag="ENO07_01435"
     CDS             complement(1978..3204)
                     /locus_tag="ENO07_01435"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00196.17,HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HDS00657.1"
                     /translation="MKVVLRMIIADDISENVELILSQLGHSAFDPQHKVVKSLKSLKS
                     ALQKSIWDIVICNYNNKKLDPLKVLQTTKGMDFEVPLIFVAESATEEEIVETIIEGAR
                     GFVTKSKISRLAPVVNRALRETAIRISAREAYKSLQDSENRYRSVIENASQGIAIIQD
                     GFLRYANPRLLEVANADEGELVDEPFIKLVYDEDRSAFNRLYLDLLRGREMDEHLDMR
                     LKSADKKLKWLRYNGVNINWNGKPAVLFFIKDISEQRLAEEKAKKSSKLIEHKSIALQ
                     EVLSQIQEDKESIKKSIYENIEQAIIPTLHRLKEIATPAQMKLFDVLEADLKRIASPF
                     IKDIKDDFAKLSPRELEVCRLIKNGMTSKEIAQALGISPMTVHKHRELIRKKLGLVNN
                     GMNLNSYLKNKMEKQK"
     gene            3478..4992
                     /locus_tag="ENO07_01440"
     CDS             3478..4992
                     /locus_tag="ENO07_01440"
                     /inference="COORDINATES: protein motif:HMM:PF04972.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HDS00658.1"
                     /translation="MRLGYAVSKIKSGNRIKTLTLSLIILLLLPVSGPAREMDDLRIS
                     YAVENQLLSEQAVPAHLINVQTNEGIVTISGSVDNLLAKARAEKVAETVKGVRSVINK
                     IVVKDIDRSDDAIEQDANRALFEDPATEAYEIEVEIRGGKAILSGRVESLQEKLLAEV
                     VVRGIRGVTDIENNIEIFYKAVRADEEIEADVRRRLESDVWVDDKLIQVEVNDGNVVL
                     TGLVGSAAEYRAARRDAWVNGTESVNTDMLEIKWEITDDMQRHEKDIGLTDDEIGEAI
                     ETAFAYDPRVSLFDITVSVNGNVATLAGTVNNLKSRMAAEDDARNTVGVRRVRNHIRV
                     RPEVIPGNQELETRVVDALARDPYVDIFDIDVAALNGKVALQGEVHTSFEKQHAEDVV
                     QKVQGVVEIENMLEYQHVWTRKSDIEISQDVEDRLYWHPFINSKQIEIEVDSGVVTLE
                     GKVDSWLEYSAAEEEAYEAGAKDVRNKLLISNLSMYELKEKLNETNRDFESDWR"
     gene            4955..5356
                     /locus_tag="ENO07_01445"
     CDS             4955..5356
                     /locus_tag="ENO07_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00659.1"
                     /translation="MRPTEILNLIGDDMSRVLLRMIPAKYKAVLALNTGGNIAWDDEL
                     RRPGIYTKEEPPERSKDPAPKDPPEPEEPPMEEPHEPEPETPVKDPDKPKEPPMEEPP
                     EDPKKPTEDKDPKSPRETPDKDPPKTPREDE"
     gene            5457..5885
                     /locus_tag="ENO07_01450"
     CDS             5457..5885
                     /locus_tag="ENO07_01450"
                     /inference="COORDINATES: protein motif:HMM:PF00571.26"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDS00660.1"
                     /translation="MKTKEIMKEDVKLIHPERNLREAARKMQQFDIGALPVVDDDKVV
                     GMITDRDILVRAVAEGGDPDQTKIGDIMTKGVVTAYDDQDVKEAKKLMKENQIRRLVI
                     LNRDNQVRGVISLGDLATAALDREESGEVLEKVSEAEHKK"
     gene            5946..6569
                     /locus_tag="ENO07_01455"
     CDS             5946..6569
                     /locus_tag="ENO07_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006912681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HDS00661.1"
                     /translation="MAKEKSKDFLKEYHKKRDFDRTAEPFGGDRRKSDGRIFVIQKHD
                     ASSLHYDFRLEIEGVLKSWAVPKGPTLDPNEKRLAVETEDHPIEYADFEGVIPEDQYG
                     GGTVMVWDAGTYENLKKDDDGKESMSMKEAYENGELTFRLKGKKIKGGFALVKTKMRG
                     KNNWLLIKKKDDEADARRNPVSSQPDSVMSGRSIEEIKKEESGEDED"
     gene            6654..7565
                     /locus_tag="ENO07_01460"
     CDS             6654..7565
                     /locus_tag="ENO07_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003767369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HDS00662.1"
                     /translation="MLATLTHDYFSDENWIYERKLDGERCLCFKSGKKVELKSRNNRV
                     LNDTYPELVQELEKQETGNFIADGEIVAFEGDVTSFSRLQNRMHSKKSGGGDVAVYYY
                     LFDLLYVEGHDITKLALRHRKSILKRAIEFSNKLRFTAHRNKEGKKYHKEACSKGWEG
                     VIAKAADSTYVHSRSKKWLKFKCVNQQEFVIGGFTDPQGERIGFGALLIGYYDDGDFH
                     YAGKVGTGYDDKLLEKLSKELKNIERKSSPFEEEVKEKGAHWVKPKLVGEVGFTEWTS
                     DGKLRHPRFLGLRRDKKARNVVREKAK"
     gene            7472..8488
                     /locus_tag="ENO07_01465"
     CDS             7472..8488
                     /locus_tag="ENO07_01465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HDS00663.1"
                     /translation="MDLRWKTASSPLPRLAAGQESQKCSQGESEVGIMSFIKAGNRKL
                     EISNEDKVFYPDSGITKGELVEYYKRAGDMIIPYMKDRPLTMRRFPDGIEGNSFYQKN
                     APKGIPDWIKTEAVKNREGGTTNHIICNDVATLIFITNYGNITPHIWLSKLDDLDKPD
                     KIIIDLDPPGDDFKAVRSAVKLIYELLTDELDLPVFLMLTGSKGVHVVLPLRPKNKFD
                     EVRDFASELADFLAQNHPRELTTEVRKSKREGRLFLDVARNAYAQTGVAPYSVRAIEN
                     APVAAPVNYGELSDKSMNAQKYNIKNIFKRMGQVKDPWKEINRHRFSLGRAREKFERL
                     KSKV"
BASE COUNT         2425 a         1760 c         2280 g         2075 t
ORIGIN      
        1 tctgcaaatc cgttgatgct caacacctta ttttttatca tataaaattc tgatccggca
       61 ggatttttgg atgtcttttc agcagagata accgagaatt ccgatggggc atctgctgcg
      121 ggtcagggaa tccactctgc ggcattggat taaactgcag aaaggagcac ggcctcaatt
      181 cgtcaagatt tgcttgtgtc ataaaaatac acggctattc ggagtggtag gatatctatt
      241 catgcggccc tccggattgc tgatttttct attaactcct ctcaagattc cgcccggcgg
      301 tgccaaccgg gcggattttt tctcattgtc gattcaaatg aaaatcgccg ggactgaatg
      361 cgtatttttt atacgtaaat tatatatgat ttaccagtat tgatttttat cccgccatcg
      421 gcaatattga atcgtgaaaa taagatggca agaacaaaaa ggtggagggt ctaatgtctg
      481 aactatattt ctttcttaat gagtatgctg ctcccaagct ggagaaagtg attgtattta
      541 cagttatatt gctggcactc taaaacctct tgaagctccg ttcgaatatt gttattattc
      601 ccggtactta aattattggc atttgcagtc tagccggaat ttcttggagg aacgagcgcg
      661 gcttgatttt gttatttcaa caatatgtat tatatttctg aatcgggatt aaaaagacaa
      721 agggagaaaa agaatgagtg attatcatga accagccgaa gagatttccg cccatgatcg
      781 caatattata cgtgctctga agagtttgcg ggaggagatc gaagcggtgg actggtacca
      841 tcagagagtg gcggtctgca aggacggaca cctgaaagcg atcctggcgc acaatcggga
      901 tgaggaaatt gagcatgcca tgatgaccct ggagtggttg aggagaaata tggatggctg
      961 ggacgaggag atgaaaacct acctgtttac agagggcgat attaccgagc tcgaggagca
     1021 tgaagaacaa tctgatgagg gagaaaaatc ttccgatctg gggatcggaa gccagaaaag
     1081 ttaattaacc gtacaataaa ggggatgata aaaggagatt gcaaatggac atattgaaac
     1141 gttcgcttgc gcctattact gacggggcct ggggtgaaat agaggatgtc gctaaagatg
     1201 tactgaaaac ccatctttcc gcccgaaaga tcgttgatat tgaaggcccg aagggcctcg
     1261 actttgccgc cgtgtctctg ggcagaatgg atattggcaa aggagagaat aagaaagaag
     1321 tcggttacgg aatccgcaaa gtccagccat tgattgaatc gaggataatt ttcgacctgg
     1381 aactgtggga actggataat atcgaacggg gtgcggatgg catcgagctg gataatctcg
     1441 aggatgccgc cagaaagatc gctcaatttg aagaagacgc tgtctatcat ggatttaagg
     1501 atggcaatat tcaggggatt aaaggtagca gcccgtacga aaaactcaaa ttcagcggtg
     1561 acccgcatga gtatttggcc ctgatatcga agggcatcac ggaatttgta agggcctcgg
     1621 tagatggacc ctatgcgctg gtcgtcggcc cggaaatgtg gaactatata cacagccaga
     1681 ccaagggcta tcccttaaaa aggcagctgg aaaacctgct cggagggaag ataatcctga
     1741 acgccttctt gaaggaatgt tatttgttct cggtgcgggg cggagacatg aagctctacc
     1801 tggggcagga tctttcaatt ggctaccatg ctcatgatac ggagaaggtg cagctttatt
     1861 ttacggaatc gtttacattc aggattattg acccggcagt tatcatggaa attgactggg
     1921 acaagtgatt ggaatataat agattgaggc cgtcatcgta gacagatggc ggctttttta
     1981 tttctgtttt tccatcttgt ttttgaggta ggagttcaaa ttcatcccat tattgaccag
     2041 ccccagcttt ttgcgaatga gctcgcgatg cttgtgtact gtcatgggcg agatgccgag
     2101 ggcctgagcg atttccttgg aggtcatgcc gttttttatg agcctgcaca cctccagttc
     2161 ccgaggagac aactttgcga aatcatcttt tatatccttg ataaaaggcg atgcgattct
     2221 cttcaaatcc gcttccaaaa catcaaaaag cttcatctgg gcgggagtgg ctatctcttt
     2281 taaacggtgc aaagtaggta ttatggcttg ttctatattc tcgtagatac ttttcttgat
     2341 gctctctttg tcttcctgta tctggcttag aacctcctgc aaggcaatac ttttatgctc
     2401 gatcagcttt gatgattttt ttgccttctc ctccgccagt ctctgctcgc tgatatcctt
     2461 tatgaaaaat agcaccgccg gcttgccgtt ccagttaatg tttactccgt tatagcgcag
     2521 ccacttaagt tttttatccg ctgatttaag gcgcatatcg agatgttcat ccatctccct
     2581 accgcgcagc aaatcaagat aaaggcgatt gaaagcggag cggtcttcat cgtatacgag
     2641 cttgatgaac ggctcgtcaa ccagttcacc ttcatccgca ttcgctacct ccagaagcct
     2701 gggattagcg tagcgcagaa atccgtcctg gataattgca attccctggc tggcgttttc
     2761 tatgactgag cggtagcggt tttctgaatc ctgcagagac ttataggctt cccttgcgct
     2821 tatccggatt gcggtctcgc gcagggccct atttactacc ggtgccaggc gggaaatctt
     2881 actttttgtg acaaaaccgc gggccccctc gataatcgtc tcgacgatct cttcttctgt
     2941 ggcgctctcg gccacgaaga tcaaaggtac ctcaaaatcc atgcctttgg tcgtctgaag
     3001 gacctttaag ggatccaatt tcttattgtt atagttgcag atcacgatat cccagatgct
     3061 tttctgaagg gcgcttttta gtgatttcag cgactttacg actttatgct gagggtcgaa
     3121 tgcgctatgg cccaactggg acagtatgag ctcgacattt tccgagatgt catccgcaat
     3181 tatcatccgc aatacaacct tcatccggtt accctttcca acataatcaa atctgcttaa
     3241 tatgtatcag gctcaaccct ctacataaat acaagaaata caaccaattg cgaaaaatca
     3301 agaaaattca gaaacattcc cctaagctga tgtgcataaa ttaattacgt attggcaata
     3361 cgtaatccgt atgtgatcaa gtcgtattgc cagaccctct gtcaatcacg atattagaat
     3421 ctgagttaga gatcgtaaga tcaaagtttg catatagttc cggaaaggag tgacgatatg
     3481 cggcttggat atgcagtttc gaaaataaag agtggtaata gaattaaaac attgacatta
     3541 tctctgatca ttcttctgtt gcttccggtc tcaggcccgg cccgggaaat ggatgacctc
     3601 aggatctcct atgcagttga aaaccagttg ctctcagaac aggcagtacc ggcccatctt
     3661 atcaatgttc aaaccaatga agggatagtg accatttcag gcagtgtgga taatctgctg
     3721 gccaaagcaa gagccgaaaa agtggcggag actgtgaagg gagtcaggtc cgttatcaat
     3781 aagatcgtcg ttaaggatat tgatagaagc gatgatgcaa tcgagcagga tgcaaaccgg
     3841 gcactgtttg aagatcctgc caccgaggcc tacgagatcg aggtcgaaat ccgcggcggg
     3901 aaggcgatat tgagcggcag ggtcgaatcg ttgcaggaga agctgctggc tgaagtggta
     3961 gtcaggggta tccgcggtgt cacggatatt gaaaataata tagagatatt ctacaaggct
     4021 gtgagggctg atgaggagat tgaagctgat gtcaggaggc gtcttgaatc ggatgtctgg
     4081 gttgatgata agttgattca ggttgaggtg aatgacggca atgtggtgct gaccgggttg
     4141 gttggaagtg cggcagagta cagggcagct cgccgggacg cctgggtcaa cgggactgaa
     4201 tcagtcaata ccgatatgct ggagatcaag tgggaaatta cagatgacat gcagcgccat
     4261 gaaaaagaca tcggcctgac agatgatgag atcggggagg ccattgagac cgccttcgcc
     4321 tatgatccgc gggtgtcgct ttttgatatc acggtctctg tgaacggtaa cgtggccacg
     4381 cttgcgggga cggtaaacaa tctcaagtcc aggatggcgg ctgaagatga tgccagaaat
     4441 actgttggtg tgcgcagggt gagaaatcat atcagagtga ggccggaagt cattcccgga
     4501 aatcaggaac tcgagacacg agtcgtggat gccctggcgc gtgacccata tgtcgatatt
     4561 ttcgatattg atgttgcggc gttgaatggt aaggtagcct tgcagggcga agtgcacact
     4621 tcttttgaaa aacagcatgc tgaggatgtg gttcaaaaag tccagggcgt ggttgaaatt
     4681 gaaaacatgc ttgaatacca gcatgtttgg acgcgcaaga gcgacatcga aatcagtcag
     4741 gatgtggaag atcggctgta ctggcatccc ttcataaatt cgaagcagat cgagatcgag
     4801 gtggatagtg gtgtggtgac tctcgaaggc aaagttgaca gctggctgga gtattcggcc
     4861 gcggaggaag aggcctacga agccggtgca aaagatgtgc gaaataaact tttaatcagt
     4921 aatctttcaa tgtatgaact aaaggagaaa ttaaatgaga ccaacagaga ttttgaatct
     4981 gattggcgat gatatgtctc gtgtattgtt aaggatgatt ccagcaaagt acaaagctgt
     5041 cctggctcta aatacgggag gcaatattgc ctgggatgat gagcttcgtc ggcccggaat
     5101 ttataccaaa gaggaacctc cggagagaag caaagatcct gctccaaagg atccgccgga
     5161 gccggaggag cctcccatgg aggaacctca tgaacccgaa ccggagaccc ctgtgaagga
     5221 tccggacaaa cccaaggagc ctcccatgga ggaacctcct gaggatccca aaaaaccgac
     5281 agaagataaa gatcctaaat caccgcggga gaccccggat aaggatccgc ccaaaacccc
     5341 cagagaggat gaataaggtg cgggtgaaag tttttctgcc aattaatcag cagtagtatt
     5401 cgctgaagcg gagttttact gattttgaat cctgaagaag aaagggagct gatgttatga
     5461 agacaaaaga aatcatgaaa gaggatgtca agctgatcca tcccgagaga aatctccggg
     5521 aagctgccag gaagatgcag cagttcgata taggcgcttt gcccgttgtg gatgatgaca
     5581 aggtcgtggg catgattacc gaccgtgaca tactggtcag ggcggttgct gaaggaggag
     5641 atcctgatca gaccaaaatc ggagatatta tgacaaaagg tgtcgtcacg gcctatgacg
     5701 atcaggatgt gaaggaagcg aagaagctga tgaaagagaa tcagatccga agactggtta
     5761 tactgaatcg ggataatcag gtcagaggag tgatctccct gggcgacctg gccacggccg
     5821 cacttgatcg ggaagaaagc ggcgaagtgc tcgaaaaagt ctctgaagcg gaacacaaga
     5881 aataggggaa taaccgggca tggcagggtg ttcctctgcc atgccgttaa tcagagtttg
     5941 aaattatggc taaagaaaaa agcaaagact ttctcaaaga ataccataaa aaaagggatt
     6001 tcgaccggac cgccgaaccg ttcggaggcg accgccgtaa atccgacggc cgtatatttg
     6061 taattcagaa gcatgatgcc tccagtctgc actacgattt cagactggag attgagggcg
     6121 tgttgaagtc atgggcagtt ccaaagggtc ccaccctcga tcccaatgaa aaacgcctgg
     6181 cggtggaaac tgaagatcac ccgatcgaat atgccgactt cgagggggta atcccggaag
     6241 accaatatgg cggcggcacg gttatggttt gggatgcggg cacgtatgaa aatctgaaga
     6301 aggatgatga tggcaaagag tccatgtcga tgaaggaagc ttatgaaaat ggagagctga
     6361 cattcaggct caagggcaaa aaaattaaag gcggtttcgc gctggtcaaa acaaaaatgc
     6421 gcggcaagaa taactggctg ttgattaaga agaaagacga tgaggcggat gcgcgcagga
     6481 atcctgtcag ttcacagcca gattctgtta tgtccgggag aagtatcgag gaaatcaaaa
     6541 aagaggaatc cggggaggat gaggattaaa aggtgaaaga actttttgaa aaattacctg
     6601 acgagcaaaa acatgaaatt aaaaagaaat cccagcctga ctggaccagc cccatgctgg
     6661 cgactctgac ccacgattac ttctcggacg agaactggat ctatgagcgc aagctggatg
     6721 gcgagcgctg cctctgcttc aaaagcggaa agaaggtcga attaaagtcc agaaataatc
     6781 gggtgctgaa tgatacctat cccgagctgg tgcaggagct ggaaaagcag gagacaggta
     6841 atttcatcgc tgatggtgag atagttgctt ttgaaggaga tgtcaccagt ttttccagac
     6901 tgcagaaccg catgcattca aagaaatccg ggggcgggga tgttgctgtt tactattacc
     6961 tttttgatct gctgtatgtc gagggccatg acataacaaa actcgcgctg cgccatcgca
     7021 agtctatcct caaaagagct atagagttca gcaataagct gcgctttacc gctcaccgca
     7081 ataaagaagg caaaaaatat cataaggaag cctgcagcaa gggctgggaa ggtgtgattg
     7141 ccaaggcggc cgacagcacc tatgtccaca gccgttcaaa aaaatggctc aagttcaagt
     7201 gtgtgaacca gcaggaattc gtgatcgggg gctttaccga tccgcaggga gaacgaatcg
     7261 gattcggtgc tcttttgatc ggctactatg acgatggtga ttttcattat gccggcaagg
     7321 tcggaacagg ctatgatgat aagctgctcg agaaactcag taaagagctt aagaatattg
     7381 agaggaaatc aagcccgttt gaagaggaag tcaaagaaaa aggagcgcat tgggtaaagc
     7441 cgaaacttgt gggcgaggtc ggattcaccg aatggacctc cgatggaaaa ctgcgtcatc
     7501 cccgcttcct cggcttgcgg cgggacaaga aagccagaaa tgtagtcagg gagaaagcga
     7561 agtaggaatc atgtcattta tcaaagcagg aaatcgaaag cttgaaatct ccaacgagga
     7621 taaagtcttt tatcccgatt ccggcataac caagggagag ctcgtagaat attacaagcg
     7681 ggccggcgat atgattatcc cgtatatgaa ggacaggccc ctgaccatgc gcaggtttcc
     7741 ggatggtatc gagggaaatt cgttttacca gaaaaacgct ccaaagggta tcccggactg
     7801 gatcaagacc gaagcggtca aaaatcggga agggggtacc acaaatcata ttatctgcaa
     7861 tgatgttgcc accctgatat ttataaccaa ctacggcaat atcactccgc atatctggct
     7921 gagcaaactg gatgatctcg ataagccgga taagataatt atcgatctcg atcctccggg
     7981 tgatgatttt aaggcggtcc ggtctgccgt gaagctgatt tatgaattat taacggatga
     8041 acttgatttg cctgtttttc tgatgttgac cggatcaaag ggagtacacg tggtgctgcc
     8101 gctcaggccg aaaaataaat ttgacgaggt gcgggacttt gcctctgagc tggcggattt
     8161 cctggctcaa aatcatcccc gggaattgac cacggaagtt cggaagagta agcgcgaggg
     8221 aaggcttttt ctggatgtgg ccaggaatgc ctatgctcag acgggtgtgg cgccgtattc
     8281 ggtccgggct atcgaaaatg ccccggtggc ggcgccggtg aattacggtg agctttccga
     8341 caagagcatg aatgcgcaga aatataacat aaaaaacatt ttcaaaagga tgggtcaggt
     8401 aaaagatccc tggaaggaga tcaatcgtca ccgtttttca ctgggcagag cccgagaaaa
     8461 atttgaaaga ctgaaatcga aggtctgaat tcgttctgga gctttgaatt ccattataaa
     8521 aggaggatgc tatgttgaaa
//
LOCUS       DSEB01000078            2315 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1007320, whole genome shotgun sequence.
ACCESSION   DSEB01000078 DSEB01000000
VERSION     DSEB01000078.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1007320"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            605..2221
                     /locus_tag="ENO07_01470"
     CDS             605..2221
                     /locus_tag="ENO07_01470"
                     /inference="COORDINATES: protein
                     motif:HMM:PF01609.19,HMM:PF05598.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE transposase"
                     /protein_id="HDS00664.1"
                     /translation="MYNDSILLQGDVAMMGQEQNQRKLFYYNVNLEGRLRIDHPLRRI
                     NEIVDFDFIYAEVKDKYGSRGNVSIPPSVILKMLLLLVFYNVRSERELMATIPERLDW
                     LWFLGYDLDSDIPNHSVLSKARKRWGVEAFRTFFERIVWQCVEAGLVDGTKIFMDSSL
                     VDANASTNSVIDTKSLRHQLKKNFKQLEARLEESNDFSRTHGPHSEANKRYISSTDPD
                     AAIVRRGKPKLRHHVHRAVDEKNEIITATRTTPGDVNEAHEMINLVEQSEEITQIEVE
                     TVVADSKYGTIENFLSCHDRNIQAHIPDLNQTKGRQKRRTIFGEDRFRYDPEEDVYYC
                     PAGQVLKRRALHKKRKSADYGAPRKMCAACELREHCTKNKSGRTIKRHLRQNELDKMR
                     QASNSAKAKRDIKTRQHLMERSFARGQRLGMKRARWRRLWRMEIQEYLTCAIQNIQVL
                     ISRVGPGKKAAINAVSIQNKETSPLPCKTGGIPLGRFSADILQRTEKVRGMLIQTICP
                     ESVCYELAIGYMQQNSFGQQPDYCQHGENS"
BASE COUNT          667 a          531 c          597 g          520 t
ORIGIN      
        1 ccggcacccc gatcgacccc ggcctgggcg ggcgacagga cattgccgcc aatgacccgc
       61 tgggacttca gttaggcaag cacaaggtaa tgtccctgag gaatatcgcc ctcaccccgc
      121 cctatggtca taatggggtc ttccagactc tggaacaaat cgtccatttc tacaatacgc
      181 gtgaccagct tcccttttgc gatcaagcac tcggcaatac cgatcccggt ttcggcacca
      241 cctgctggcc ggaaccggaa gtccctcgga acgtgaatac ggccgaactt ggactcctcc
      301 gcctcacgcc acaacaggaa gcggatatcg tcgcctttct taataccctt accgacggct
      361 ggggaccgga aaacggcatg gctcccttgc cccgcccggt gatgccgccg accccgtgaa
      421 aatggtctta ggcacctgcc cgactgcagg ccccctatac ggtgggcctg cagcggttcc
      481 ttgggtggag acggggagat ggtgtcgtgt caaatgctta cgggctgttg cccaaatata
      541 aagtcaaagt tgatattaac aaatatgaaa tagctaaatt atacttacaa ttatcagcta
      601 aattatgtat aatgacagca tcttgctaca aggagatgtt gccatgatgg gacaagaaca
      661 gaaccagaga aaattgttct actacaatgt caatctcgaa gggcggttac ggattgatca
      721 tcctctccgc aggataaacg agatcgtaga ttttgatttc atttatgccg aggtaaagga
      781 taaatacggc tcgcgcggta atgtttccat acctccttcg gtaatattaa aaatgctttt
      841 gctgctggtt ttctataacg tccgttcgga acgggaattg atggccacga taccggaacg
      901 gttggattgg ttatggtttt tgggatatga cctggattcc gatattccga accacagtgt
      961 tctgtccaag gcccgaaaac gatggggtgt ggaggcattt aggacttttt tcgaacgcat
     1021 tgtctggcaa tgcgttgaag cgggactggt ggatggcacg aagattttca tggattcaag
     1081 cctggtcgat gccaatgcct caacaaattc ggtgatcgac accaaaagcc ttaggcatca
     1141 attaaagaag aatttcaagc aacttgaagc ccgcctggaa gagtcgaacg atttttcaag
     1201 aacccacggc cctcatagcg aggccaataa acggtatata tcgagcacag atcctgatgc
     1261 cgcgattgtt cggcggggga aaccgaaatt acgacaccat gttcaccggg cagtagacga
     1321 gaagaatgaa attataactg caacgaggac tacgccgggt gatgtgaatg aagcccatga
     1381 gatgataaat ctggttgagc agtcggagga aattacgcaa atagaagtag agacggtggt
     1441 ggcggacagc aagtatggaa cgattgagaa tttcctgagc tgtcatgacc ggaacattca
     1501 agcgcatatt cctgatttaa accagacaaa gggaagacaa aagagacgaa caatatttgg
     1561 cgaagatcga ttccgttatg atcctgaaga ggatgtatac tactgccctg ccggtcaggt
     1621 gttaaaacgc agagcgttgc acaaaaaaag gaaaagtgct gattatggcg ctccccggaa
     1681 aatgtgtgcc gcctgtgagt tgcgggagca ttgcacgaaa aacaaatccg gtcgaacaat
     1741 aaaacggcat ctccggcaaa atgaactgga caagatgcgt caggcgagca acagcgcaaa
     1801 ggcaaagagg gacataaaga ccagacagca tctcatggaa agatcttttg ctcgtgggca
     1861 acgattggga atgaaacggg caagatggcg gaggttatgg cggatggaaa tccaggaata
     1921 tctgacctgc gcaatccaga atattcaagt tttgatttca cgtgtcggtc cgggcaaaaa
     1981 agcggcaatc aatgctgtca gcatacagaa taaagaaacc agtcctttgc cctgcaaaac
     2041 aggaggtatt cctttgggga gattttcagc agatattctt caaaggacag aaaaagtcag
     2101 ggggatgttg attcaaacca tctgtcccga atcagtttgt tacgagctgg cgattggata
     2161 tatgcagcaa aatagctttg ggcaacagcc cgattactgt caacacggcg aaaactcctg
     2221 aaattcactt aataataagc acttaggttt tcaactgatc caaccgcagg ggaaaaccag
     2281 ccaccagccg gagattctgt tcgtggttat gctaa
//
LOCUS       DSEB01000079            8594 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000420, whole genome shotgun sequence.
ACCESSION   DSEB01000079 DSEB01000000
VERSION     DSEB01000079.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8594)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8594)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8594
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000420"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1178
                     /locus_tag="ENO07_01475"
     CDS             <1..1178
                     /locus_tag="ENO07_01475"
                     /inference="COORDINATES: protein motif:HMM:PF00982.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HDS00665.1"
                     /translation="NVNRRFAEKIVESAEPGDLIWIHDYHLILVAHYLRQMNVQHPLA
                     FFLHIPFPNYDLLRRLPWKDHIIGGLLDYDLVGFQTRRDRRNFCRCAKELMPSLDIKA
                     YYRHSNVRFGRREIKVGNFPISIDFSEFSDGAQTEEVEKEANYLKQKCQSKSLMLGVD
                     RLDYTKGIPERFLAYERALEKYPELRGNICLLQLAVPSRSHLSAYQDIRETLEWMVGR
                     INGRFSEFGWQPIQYIYRSVSRTELLGFYRASDIGLLTPLRDGMNLVAKEFCASTVDN
                     NGVLILSEFAGASEQLSKGAIMVNPYNREETADAIYQAFHMDLAERERRMTLLRSEVK
                     RNDIFRWVEWFLSSFRESEREGAASAIHPANSQSVRSRVESAVMESIKNGGNSYTDD"
     gene            complement(1175..3364)
                     /locus_tag="ENO07_01480"
     CDS             complement(1175..3364)
                     /locus_tag="ENO07_01480"
                     /inference="COORDINATES: protein motif:HMM:PF13361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase PcrA"
                     /protein_id="HDS00666.1"
                     /translation="MNKFLEKIKSDLNPEQLKAVVYTDGPLLILAGAGSGKTRVLTYK
                     AAYLVHEKNIPPYRILAVTFTNKAANEMKTRLIDLAGPSADQMRVSTFHSFCLRVLRR
                     YADRIGYEKSFGVYDQDDSLNLIKRCMESLDISVKTHAPRAVAEYISRAKEIMVDPEE
                     YEKTAAAYFNKIVSKVYKVYQQQLRKSNVFDFDDLLFWTVYILQNHEDVREKLQNHFQ
                     HVLVDEYQDTNHVQFLLVKILAEKSRMLTVVGDDDQSIYGWRGADIRNILDFEASFPD
                     CRIIKLEQNYRSTKMILRTASEVVKYNRSRKGKTLWTEGEEGELIKLIKVNSDRNEAE
                     MICEDIENMVASDKYKRRDIAILYRTNAQSRALEESLKNRFIPYTIVGGIRFYQRKEI
                     KDILAYLKMLVNPYDIISFRRIINFPKRGIGPVNIARIEQAAVRRSTSPLELLLNAEE
                     LDFLKGAALNGAKQFQRIYKELVRAKKEQSPVELAETAARISGIVDSLSEYDQIEAES
                     RKENIDELIAAVQEYCDKVENATLEGFLEEIALYTDVDTWDSSQDVVTLMTLHSAKGL
                     EFPKVFITGLEEGLFPLSRSMEKPEELEEERRLFYVGITRAEKSLTLSYAQRRMRFGE
                     MLSIKSRFIDELPEECIEFEDQTFSSSYPGTYTGSERAHYDEQNYVAPNDKYRDLKVG
                     KRIIHPTWGEGKIVSRTGSSDNTTVEVRFRWGGKKKLFAKYANLKLI"
     gene            3459..4283
                     /gene="mutM"
                     /locus_tag="ENO07_01485"
     CDS             3459..4283
                     /gene="mutM"
                     /locus_tag="ENO07_01485"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:PRK01103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDS00667.1"
                     /translation="MPELPEVETVVRGLQGCVAGKTISEIEYCAPHLRKLNLRGFAGK
                     VAGRIIDSIDRIGKNIFINLDDGAVFLVHLRMTGQLLYCCQQVPEDNHNHLKMNFVGN
                     GEALLFRDVRKFGHFIHIPAKKKSAYIESMGLGEDALKIEADSFVEVLKSKKRMIKPL
                     LLDQSVVAGLGNIYVDEILHRSKVHPQKISSGIASRKLREMYDHMQRILRFSIENMGT
                     TFDSFSGVNSEPGRNQNYLLAYNNAGKLCSLCGKRKIKRIVVAQRGTFICTNCQRP"
     gene            complement(4319..5134)
                     /locus_tag="ENO07_01490"
     CDS             complement(4319..5134)
                     /locus_tag="ENO07_01490"
                     /inference="COORDINATES: protein motif:HMM:PF13490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00668.1"
                     /translation="MKCRQALKLLYDFLDNQLDNKSVEEVKEHLSQCKHCFETYTFEE
                     HLNQFIRTKCCHQEQEQLSAVDRLKQQVKHRIEQLEEEPEENPEETEGNSQRFFLFRR
                     PVFAVGFFAIIAIISFALYIGNSNQTAWADDFIKNHDLAVRGENLLDITTHDPHMIDS
                     CLSAKMSLPKGIFLREMGFQPNGGKVADDNERPCAQIVYSVQGHPISIFVMNLGDFTR
                     PAGLQKLPGDEGTYYYKMGEHRLLLWNCSKYWYVATGDADDDLMLDFRSHFAN"
     gene            complement(5131..5685)
                     /locus_tag="ENO07_01495"
     CDS             complement(5131..5685)
                     /locus_tag="ENO07_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDS00669.1"
                     /translation="MDHTDALYRTALRMTKNTKDAEDLVQETLLKAYRFFDRFEKGTN
                     IRAWLFKIMMNIFINDYRQKSKKPTSMSYEDVDDNYLYHQLKFQKTEDGDPEGSLFSR
                     IFDDDVKRAIEELPDDFRIVVVLAFLEEFSYQEIAEIAGLQLGTVKSRLHRGRKLLQK
                     SLWDYAVRNGFLKTKNKSVKGEEK"
     gene            complement(6089..6964)
                     /locus_tag="ENO07_01500"
     CDS             complement(6089..6964)
                     /locus_tag="ENO07_01500"
                     /inference="COORDINATES: protein motif:HMM:PF05368.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDS00670.1"
                     /translation="MILVTGATGKIGSRLAKHLMESGNRIRVVSRSEEKIEDFKKKGA
                     EPAVGNLLDRDFCLRAFDGCGRAFLLVQGDPASGRHYEEEVQIGKNYAEALRTTDIEH
                     AIFISSLGVEKGTGSALIDSKIQIEADLKDTGIPVTVIRPGNFLENMYNFFETISSAG
                     FFTYPMPGNIKIPHVSVDDIAEIAFKAFRRGPNGWEIIDMPGIEYSFFDIAMEISEKL
                     NRMIKYMRVSDDQFREVFTGFGISDKFCDDYLAMFRYFEEGDFNYDPQSMPSEFDYSP
                     KTLEEFVPELVRAIK"
     gene            7141..7422
                     /locus_tag="ENO07_01505"
     CDS             7141..7422
                     /locus_tag="ENO07_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00671.1"
                     /translation="MITIDVHEAFKMLIWCVILFSLGIAAFLDSLFNYGDIFRRVNSV
                     LFMLIALGLLVRTTTKMKTRRMENYIEKVDDLRAEIKDLRNKKGSQAGI"
     gene            7480..7857
                     /locus_tag="ENO07_01510"
     CDS             7480..7857
                     /locus_tag="ENO07_01510"
                     /inference="COORDINATES: protein motif:HMM:PF07238.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HDS00672.1"
                     /translation="MDNIKRKSREVPNDYFLVYEHQKNEFMGRLLNMSLGGMMLVSEG
                     PVEIEKLFSCRMKLPEVIYGHDYIVFEAESRWCKMNNNTKMYETGYQLKNVSDEVEKM
                     IAILMKIWSSANDNSPKTWPMKF"
     gene            8012..8449
                     /locus_tag="ENO07_01515"
     CDS             8012..8449
                     /locus_tag="ENO07_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00673.1"
                     /translation="MCPRFMIALLFISALYGGYLCAQDSELGESEAAPEDSIIQDTAF
                     IDSSASSGREVNLLIEEFTGTNKIGQFLQQQRYRLNYSLDSIPEERIDEYVADMRARR
                     SVKSTIYGIYKPIPMREWPWIMEFSSNVYTGFKPVNPLVIFGY"
BASE COUNT         2210 a         2040 c         1985 g         2359 t
ORIGIN      
        1 gaaatgtcaa tcggagattc gcggaaaaaa tcgtggagag cgcggaaccc ggcgatctta
       61 tctggattca tgattatcat ctcatattgg tggcccatta cctgagacag atgaatgtgc
      121 agcatcccct ggctttcttt ctgcacatcc ccttccccaa ttacgacctt ttacgccgac
      181 tgccctggaa ggatcacatt atcgggggac tcctggatta tgatctggtc ggattccaga
      241 cccggcggga caggcggaat ttttgccgct gcgccaagga attgatgccc agcctcgata
      301 tcaaggctta ttaccggcac tcgaatgtgc gctttgggcg gcgcgagatc aaggttggta
      361 attttcccat cagcatcgat ttctccgagt tcagtgacgg cgctcaaacc gaagaagttg
      421 agaaggaagc gaattatctt aaacagaaat gtcagtccaa aagtctcatg ctgggggtcg
      481 accgtctcga ttacaccaag ggaatacccg aacgtttttt ggcctacgaa cgcgcgctgg
      541 aaaagtatcc tgaattgcgg ggaaatatct gcctcttaca actggctgtc cccagccgct
      601 cacatttatc ggcttaccag gatattcgcg aaacgctgga atggatggtg ggccggatta
      661 acggccgttt ctcggaattc ggatggcagc cgatccagta tatctaccgt tcggtcagcc
      721 gcacagagct gctgggattt taccgcgcca gcgatatcgg gcttctgaca cctttacggg
      781 atggtatgaa tctggtggcc aaggaatttt gcgccagcac tgtcgataat aacggtgtct
      841 tgattttgag cgagttcgcc ggagcctccg agcagctttc caaaggtgcg ataatggtaa
      901 atccttataa cagggaggaa accgctgatg caatctacca ggccttccac atggacctgg
      961 ctgaacgcga aagaaggatg actctcctgc gatccgaagt caagcgcaac gatatatttc
     1021 gctgggtgga atggttcctg tcatcgttca gggagtccga gagggaaggt gccgccagtg
     1081 caattcatcc cgctaacagc cagtcggtcc ggagccgagt tgagtcggca gtgatggaat
     1141 cgataaaaaa cggcggcaat tcgtatacgg acgattaaat aagctttaga ttggcgtatt
     1201 tcgcaaacag ctttttttta cctccccacc tgaaacgcac ttccactgtg gtgttgtcgg
     1261 atgatccggt tcgtgagaca attttgcctt ctccccaggt aggatgaata attctttttc
     1321 caactttcaa atccctgtat ttatcattcg gggcgacata gttttgttca tcataatggg
     1381 cccgctctga accggtgtag gttccgggat aggatgagct aaaagtctga tcttcaaatt
     1441 caatgcattc ctccggaagt tcatcgataa aacgggattt gatggagagc atctcgccga
     1501 atctcatgcg cctttgtgca tagctcaacg tcaggctctt ctcggcgcgg gtaataccga
     1561 cataaaagag acggcgttct tcctccagct cctccggctt ctccatgctg cgtgagagcg
     1621 gaaaaagtcc ctcctcgagg ccggtaataa agactttcgg aaattccagt cccttggcgc
     1681 tatgcagggt catcagcgtc accacgtcct gtgagctgtc ccaggtatcg acatcggtgt
     1741 aaagcgcgat ctcctccagg aagccctcga gtgtggcatt ttcgacctta tcgcaatact
     1801 cctgtaccgc cgctatcaac tcgtcaatat tttcttttct ggattcagcc tcgatctgat
     1861 cgtattcgct gaggctgtcc acaatccctg agattcgcgc ggcggtctca gcaagttcca
     1921 ccggcgactg ctcctttttg gccctgacca gttctttata tattctttga aattgttttg
     1981 caccattcaa ggccgcgccc ttgaggaaat cgagctcctc ggcgtttaag agcagttcaa
     2041 gaggcgaagt cgagcggcgg actgccgcct gctcgatacg cgcgatattg accggaccaa
     2101 tgcctcgttt ggggaagttt attattcttc taaaagatat aatatcataa ggattcacca
     2161 gcatcttcaa atacgctaga atatccttga tctccttgcg ctgataaaaa cggatgcccc
     2221 cgacaatggt gtagggaatg aacctgtttt tgagcgattc ttccagcgcg cgggactggg
     2281 cgttggtgcg gtataaaatt gcgatatcac gtctcttata cttgtctgag gcaaccatgt
     2341 tttctatatc ttcgcagatc atctcggcct cgttgcggtc ggagttgacc ttgatcagtt
     2401 tgataagctc cccttcttct ccttcggtcc ataatgtctt gcctttacgg gagcggttat
     2461 attttaccac ctcggaggcg gtcctcagga tcatctttgt ggagcggtaa ttctgttcca
     2521 gttttatgat cctgcagtcc ggaaagctgg cttcgaaatc caggatattt ctgatatccg
     2581 ctccccgcca gccatagata gactggtcat catcccccac caccgtgagc atgcgtgatt
     2641 tttcagccag gattttcacc agcaggaact ggacgtggtt ggtgtcctgg tattcatcaa
     2701 ccagaacatg ctgaaaatga ttctgcagtt tttcgcgcac gtcctcatga ttctgcagaa
     2761 tgtaaactgt ccagaatagg agatcgtcga aatcgaagac attgcttttg cgcaattgct
     2821 gctgatacac tttgtagact tttgagacta ttttattaaa ataggctgcg gctgtttttt
     2881 cgtattcctc cgggtcgacc atgatttcct tggcccgact gatatattcc gccaccgccc
     2941 gaggagcatg agtttttacg gatatatcca gtgattccat gcagcgcttg atcaggttca
     3001 gcgagtcatc ctgatcatag actccgaagc tcttctcata tccgattcgg tcggcataac
     3061 gtctcaacac ccgcaggcag aatgaatgaa acgtggatac ccgcatctga tctgcagagg
     3121 gacctgccag gtcaataagc cgggttttca tctcattggc ggccttattt gtaaatgtca
     3181 ccgccaggat gcgatatgga gggatgttct tttcatgcac caggtaagcc gccttgtagg
     3241 taagtacccg ggtcttgccc gatccggcgc cggccagtat caagagcggg ccgtcggtat
     3301 agaccaccgc cttcaattgc tcgggattca agtcggattt gattttttcc aggaacttat
     3361 tcatagatcg cctaatatat ctaaatacga ttagtagtca atcatgaagc gcataaatta
     3421 attgccagta aggaaaattt gtagtattta catgcattat gcctgaatta ccggaagttg
     3481 agacagtggt caggggtctg cagggctgcg ttgccggaaa gaccataagt gaaatagaat
     3541 attgcgcacc gcatctgcgt aaactgaacc tgcgcggctt tgcgggcaaa gttgcgggaa
     3601 gaattattga ttcgatcgac cgaatcggga aaaacatatt tattaatctt gatgacggcg
     3661 cggtgtttct tgtccatctc aggatgaccg gccagcttct gtattgttgc cagcaggtcc
     3721 cggaagataa ccacaatcac ctgaaaatga actttgtggg taacggcgag gcgcttctct
     3781 ttcgcgatgt gcgtaaattc gggcatttca tccatattcc agccaagaaa aaatcggcat
     3841 acatagagag catgggcctg ggagaggacg ccttgaaaat tgaggcggac agttttgttg
     3901 aggtactgaa atcaaaaaag cggatgatca aaccgctgct tttggatcaa tctgtcgtgg
     3961 ccggcctggg gaatatctat gttgatgaaa tcctgcacag gagcaaagta cacccgcaaa
     4021 aaatatcctc aggcatagcc tcccggaaat tgcgtgagat gtatgaccat atgcaaagga
     4081 ttctgagatt ctcgatcgaa aatatgggta caacatttga ctcattctcc ggcgtcaact
     4141 cggagccggg caggaatcag aattatctgc tggcctataa taatgccgga aagctatgta
     4201 gtctctgtgg taaaaggaag atcaagcgga ttgtagtggc gcagagaggt actttcatct
     4261 gcacaaactg tcagcgacct tagccccttt tagcttattt gatgtagatc cccgaaaatc
     4321 agttggcaaa gtgcgatctg aaatcaagca tcagatcatc atccgcatca cctgtcgcca
     4381 cataccagta cttgctgcaa ttccagagca gaagcctgtg ttcgcccatt ttataatagt
     4441 atgtgccctc gtcaccgggg agcttctgca aaccggcggg tcgggtaaaa tccccaagat
     4501 tcataacgaa aatagatatc ggatgcccct ggacactata tactatctgg gcacatggtc
     4561 tctcgttatc gtcagcgact ttcccgccat tgggctgaaa acccatttcc cgcaggaaaa
     4621 tgcccttggg caggctcatc ttggccgaaa gacatgaatc gatcatgtgt gggtcatgag
     4681 tggtaatgtc cagcagattt tctcccctga cggctaagtc atggttcttt atgaagtcat
     4741 ctgcccaggc tgtctgattc gaattgccga tataaagggc aaatgatatg atagcgatta
     4801 tcgcaaagaa ccccacagca aaaaccggcc gtctaaatag aaaaaaacgc tgggagtttc
     4861 cttcggtttc ctcaggattc tcttccggct cttcttccaa ttgttcaatg cgatgcttaa
     4921 cctgctgttt cagacgatca acagcgctga gctgttcctg ctcctgatgg cagcattttg
     4981 ttctgatgaa ctgattgaga tgttcctcga aggtgtaagt ctcgaagcaa tgcttacatt
     5041 gggacagatg ttctttcact tcttcaactg acttgttatc aagctggttg tcaaggaaat
     5101 catagagtaa tttcaatgct tgacggcact tcatttttct tcacccttca cagatttatt
     5161 ctttgttttc aaaaaaccat ttctcactgc ataatcccat aagctcttct gcaaaagctt
     5221 gcgcccccta tgcaacctgg acttgacggt gccaagttga agacccgcta tttctgcgat
     5281 ttcctggtaa gagaattcct ccaaaaatgc cagtaccacc acaattcgga agtcgtcagg
     5341 gagctcttcg atggcccttt tgacatcgtc gtcaaaaatc ctcgaaaata gagatccctc
     5401 cggatcgccg tcctccgttt tctggaattt caactggtgg taaagatagt tatcatcaac
     5461 atcctcataa gacatggagg ttggtttttt gctcttctgc cgataatcat tgatgaaaat
     5521 gttcatcatt atcttgaaga gccaggccct tatgttagtg cctttttcaa atctgtcgaa
     5581 gaatctgtag gcctttaaca aagtctcctg taccaagtcc tccgcatctt tagtattctt
     5641 tgtcatcctg agggcggttc gatacagcgc atcggtatga tccatggctg tctcttcgaa
     5701 ttccctctgt ttcttgtgat cgtccgactt actcacaaat ttctaagttg ttttcagcca
     5761 gagtgaagag tcacacttct gactaaaata acaactcctc caagttttaa gttcccaaaa
     5821 tatgctctat aaagataaaa ccatagctaa cttaatcaag ccatttcttt tattggtatt
     5881 ctcaggatga gtggttacag cccgtgcttt tattgtctca ccctcgccag ccgcttaaac
     5941 acgataaatt tgtcaagaat attatactgc aatactcctt gtttcgcaat cttatttttt
     6001 atactcacca acccccaaaa aggtcagagt aatcttcata aaataagtcc tatttcccct
     6061 gcagaaatcg tatatactcg caaattgttt actttattgc ccttacaagc tccggtacga
     6121 attcctccag ggtctttggg gagtaatcga attctgacgg catgctctgt ggatcgtaat
     6181 tgaaatcccc ttcttcaaaa tatctaaaca tagccaggta atcatcacaa aatttatcgg
     6241 agattccgaa tccagtgaaa acttcacgga actgatcatc cgaaacacgc atatacttta
     6301 tcattctgtt caacttttcc gaaatttcca tggcaatgtc gaagaaactg tattcaattc
     6361 cgggcatatc gattatttcc catccgttgg gtccgcgccg gaaagccttg aaagcaatct
     6421 cggcgatatc atcgacagaa acatgcggaa tcttgatatt gcccggcatg ggataggtga
     6481 agaatccggc tgatgaaata gtctcaaaga aattatacat attctccaga aaattcccgg
     6541 ggcgaatcac ggttaccgga atccccgtat ctttaagatc ggcttcgatc tgtattttgg
     6601 aatcgatcaa tgctgacccg gtacccttct caaccccgag ggagctgatg aatattgcat
     6661 gctcgatatc cgtggtccgc agcgcttccg cgtaattttt gccgatctgg acttcctctt
     6721 cgtaatgtct gccgctggct ggatccccct gcaccagaag aaaggcacgg ccacatccat
     6781 cgaaagccct gagacagaaa tcccgatcga gcagatttcc aaccgccggt tcggcccctt
     6841 ttttcttaaa atcctcgatt ttttcctcgc tcctggatac tacccggatc cggtttcctg
     6901 attccatgag atgttttgcc aggcggctcc cgattttacc ggtggctccg gttaccaaaa
     6961 tcatttcagc gctcctgttt aagggtaatc tgatccgcag cgaaatatca aagccaaggt
     7021 aagcaataaa attcgccagg caagcgaaaa tggacacatc ctgcccggat gagaagttga
     7081 acctccggaa aagtatcaca aagactatta aaacttggaa ttatatagcc gataagatca
     7141 atgataacta tagacgtgca tgaggctttt aaaatgctta tctggtgtgt tatattgttt
     7201 tcactgggaa ttgcggcctt tctggatagt ctctttaatt acggggatat attcaggcgt
     7261 gtaaattctg ttctttttat gctgattgct ctgggtctcc ttgtccgcac caccacgaaa
     7321 atgaaaacac gcaggatgga aaactatatt gaaaaggtcg atgacctcag ggctgaaatc
     7381 aaggatctca gaaataaaaa agggtcgcaa gcaggcatct gacgagaatc atattagccg
     7441 gcctgcaaat tgatcctggt caaatgagga tatacagctg tggataacat caaaagaaag
     7501 tcacgagaag ttcccaacga ttactttctg gtttatgaac atcaaaaaaa tgaattcatg
     7561 gggcgcctct tgaatatgtc cctgggcggt atgatgctgg tctccgaggg tccggtggaa
     7621 atcgagaagc tcttcagctg ccgaatgaaa ctgcctgagg tgatatacgg gcatgattat
     7681 atcgtcttcg aggccgagag cagatggtgc aagatgaata acaataccaa gatgtatgag
     7741 accggatacc agctaaaaaa tgtctcggat gaagtcgaga agatgatcgc tatccttatg
     7801 aaaatctgga gttctgcgaa cgacaacagt ccgaaaacat ggcccatgaa attctagtag
     7861 agtacattct tttattctat tgtatcccga aaatgaactt tttcccgcaa tcttttttgc
     7921 gggatttttt ttgaaaatgc gggccgcttt ttccattata ttgtaaaaca gtataacgat
     7981 cgatggccat ttaaaaggcg gattgaatat catgtgtcct cgattcatga ttgctcttct
     8041 gtttatctcg gctctatatg gaggatacct ttgtgcgcag gatagcgagc ttggtgaaag
     8101 tgaagcggcg cctgaggact caatcataca ggatactgca tttattgatt cgagtgccag
     8161 ttccggccgc gaggtcaatc tgctcattga ggaattcacc ggcaccaaca agataggcca
     8221 gtttcttcag caacagagat acaggttgaa ttacagtctg gacagcatac cggaagagag
     8281 gatcgatgag tatgttgccg atatgcgtgc gcggcgctcg gtcaaatcaa cgatctatgg
     8341 aatctacaag ccaatcccta tgcgtgaatg gccctggatc atggaatttt cgagcaatgt
     8401 ctataccggc ttcaagcccg tcaatccgct ggtgatattc ggatattaag agcggtgata
     8461 tgattattgt tatgggtgcc ccatccccgt tgagccgctc acggctcata aggggtggga
     8521 cttatgttaa ataattgaat tccatgaaga ctaaaatgca accgcttatg cagtggggca
     8581 ccaaaaggta aaca
//
LOCUS       DSEB01000080            5566 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001031, whole genome shotgun sequence.
ACCESSION   DSEB01000080 DSEB01000000
VERSION     DSEB01000080.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5566)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001031"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..693)
                     /locus_tag="ENO07_01520"
     CDS             complement(<1..693)
                     /locus_tag="ENO07_01520"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008794370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HDS00674.1"
                     /translation="MDKVEKIWMNGKLVNWDDAKIHVLSHGLHYGSVVFEGARCYKTD
                     KGAAIFRLQEHTDRLFNSAKICRMEIPFTKEQINDAIIELIKINKLEECYIRPIVYRG
                     YHSLGVFPGDCPIDAAIAVWKWGKYLGPEALEKGVNVCVSSWNRPAPNTLPAMAKSGA
                     NYLGGQLIKMEAIAHGYVEGIGLDVEGFVSEGSGENIFIVRNGTLITPPFCASILAGI
                     TRRTVIRLAEEMA"
     gene            complement(705..1847)
                     /locus_tag="ENO07_01525"
     CDS             complement(705..1847)
                     /locus_tag="ENO07_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDS00675.1"
                     /translation="MDFDIKYKDFRKEIRDYVEEKIAPHAAEVDREQFYSREIHQAVN
                     DKGWWGSTVPKEYGGLGLSTVEYAIIVEEISRVCGSTGLTFAAHNSLGTFPIYAFGTE
                     EQRKKYLPDGAKGSLIAFGLTEPEAGSDAGGTKSRAEKSNGSYILNGTKCWITSAETC
                     DYAILTARTSDEGGVKGISSFILEKGMEGFSVGKKENKCGCRGSNTAYMHFDDLKVPE
                     SQRLGEEGMGFKQFMITLDGGRISIGAMALGLAQAALELALEYSESHIENGKPINRNQ
                     GIQFKLADMGTQVEAARLLIYKTAWMKDNKIPYTKYSAMCKLYASEVANFCTATAIQI
                     LGLDGISEDYPASRYFRDMKLCEIGEGTSEIQRIVIARELLKDLQK"
     gene            complement(1850..2992)
                     /locus_tag="ENO07_01530"
     CDS             complement(1850..2992)
                     /locus_tag="ENO07_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000545520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDS00676.1"
                     /translation="MNFDLSEDHIMMRDFGREFAAKRLFDIAEEMDAKEYMPDEVIEE
                     AKELGFFGLLAPEEYGGEGIDTLSYALIIEELSKACAGFAIMISVHNSLVMKAIEYFG
                     TDEQKKEYLPKLASGEIIGAYSLSEPGSGTDAGSLKCQAIAEGDHYVFNGTKSWVTSA
                     QRAGIIIVFALTDPEKGPKGISAIIVPAGTEGMSLGAPEKKMGLKCSDTREVSFIDAK
                     VPKANLIGEENHGFKVALSLLDNGRIGVAAQALGIAESAFREAFNYSKERKQFGRPIS
                     EFQAIQFKLADMATQIDAAKLLVYRAAVLNDAEGLHSREISMAKLFATEMSNYVVDEA
                     VQIHGGYGYVKEYPVERYFRDARVTEIYEGTSEAQRIVISRNLLKG"
     gene            complement(3004..3858)
                     /locus_tag="ENO07_01535"
     CDS             complement(3004..3858)
                     /locus_tag="ENO07_01535"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009332307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HDS00677.1"
                     /translation="MEISNVAVFGGGTMGNGIAHVFAQNGYKVTLIDIKQEFLDKAIS
                     TIEKNMQRQVKKEVITEDQMKESLTRITTSTNEKDAEGSQLVIEAIIEELDPKLKLFK
                     KIDSIAAPEVIIASNTSSLPITKLASATGRADRVIGMHFMNPVPMMKLVEVIRGVATS
                     DDTYRTVEELAKKLGKTPVEVNDYPGFVANRILLPMINEAVWALFEGVATEEAIDTVM
                     KLGMAHPMGPLTLADFIGLDVCLAILEVMHEGFGDPKYAPCPLLTKMVEAGYLGRKTG
                     RGFYDYSK"
     gene            complement(3898..5088)
                     /locus_tag="ENO07_01540"
     CDS             complement(3898..5088)
                     /locus_tag="ENO07_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDS00678.1"
                     /translation="MDYPEAYIVAGCRSAIGVLHSHLSAFTAPQLGAFAIKEVVKRAN
                     IDPATIDEVIMGQVVQGGSGQAPGRQAAIHGGIPPEVPAITINKVCGSGLKAVMMAAS
                     QIKAGDGHCFVCGGMESMSNAPNVLPAAAKRGFKFGDQKLLDIMVYDGLWDSFNNHHM
                     GNSAEYTADKAGITREEQDEFAYNSHQKALKAWADGKFDEEVFPIEIPQRKGDPVVFN
                     KDEGPRDTPLEKFAKLRPAFVKDGTVTAGNAPGLNDGSAAVVVCSKEYIDKHGLKPIA
                     KITAYDSASLDPKDLFFTPIYAVRKVMKKLDQKDINYFDLIEANEAFSAQALADGKEL
                     GWDWDRVNVHGGAVALGHPIGASGARVLVTLMHALKDRGKKTGIATLCLGGGSAVALS
                     IEMV"
BASE COUNT         1277 a         1539 c         1335 g         1415 t
ORIGIN      
        1 agccatctcc tcggccaggc gaataaccgt gcggcgagtg atacctgcca gaatcgaggc
       61 gcagaagggc ggcgtaatca aagtgccgtt cctgacaata aagatattct cgcccgaacc
      121 ttcggagaca aaaccctcga catcaaggcc gattccctcg acatagccgt gcgcaatagc
      181 ttccatcttg ataagctgtc cgcctagata gttggcgccg gattttgcca tcgccggcag
      241 agtgttggga gcgggacggt tccaggagga cacacagacg ttaacgcctt tctccagggc
      301 ctccgggcca agatattttc cccatttcca gaccgcaatg gcggcgtcga tcgggcaatc
      361 cccgggaaat actccaagcg aatgatagcc gcggtagact atgggacgaa tatagcattc
      421 ctcgagcttg ttgatcttga tcagctctat aatagcatcg ttgatctgct ctttggtgaa
      481 cggtatctcc atgcggcaga ttttggccga gttgaagagc ctgtcggtat gttcctgaag
      541 gcggaagatc gccgccccct tgtcggtctt gtagcagcgc gcgccctcga aaacgaccga
      601 gccgtaatgg aggccgtggg agagcacatg aattttggcg tcatcccagt tgaccagctt
      661 accattcatc cagatttttt ctactttatc cataggcagt ctccttattt ttgcagatct
      721 ttcaaaagct cccgggcaat cactatccgc tggatttccg aggtaccctc gcctatttcg
      781 cacaatttca tgtcgcggaa ataacgggag gcgggataat cctccgaaat accatcaaga
      841 ccaaggatct gtatcgccgt cgctgtacag aaattggcga cctcggaagc atacagctta
      901 cacattgctg aatattttgt atatggaatt ttattatcct tcatccaggc tgttttgtag
      961 atcaggagac gggcggcctc aacctgggtt cccatgtcgg ccagcttgaa ctggattccc
     1021 tgattgcggt tgatcggctt accgttctcg atatggctct cggagtattc cagcgccagc
     1081 tcaagtgcag cctgagccag acccagagcc atagcgccga tcgatattct gccaccgtca
     1141 agagtgatca tgaactgctt gaagcccatg ccttcttcac ccagtctctg tgattcgggg
     1201 actttaagat cgtcgaagtg catgtaggct gtgttagagc cgcgacatcc gcacttattt
     1261 tctttctttc cgactgagaa tccctccatg cctttttcga gaataaatga ggatataccc
     1321 ttgacgccgc cctcatcgct ggtacgcgcg gttagaatgg cataatcaca ggtctctgca
     1381 gaggtgatcc agcacttggt accattaagg atatagctgc cgttcgattt ctctgcacgg
     1441 ctcttggtac cgccggcatc cgacccggcc tccggttcag tcagcccgaa tgctatcagg
     1501 ctgcctttgg cgccatcggg aagatatttc ttgcgctgct cctcggtgcc aaaagcgtag
     1561 atcgggaatg tccccagcga attgtgggcg gcaaaggtca atccggttga accacagaca
     1621 cgtgatatct cctccacaat tatagcatat tcaaccgtgg agagtcccag acctccatat
     1681 tctttcggga cagtcgatcc ccaccagcct ttgtcattaa ccgcctgatg aatctctctg
     1741 gagtaaaact gctccctgtc aacctcagcg gcatgcgggg caatcttctc ctcgacatag
     1801 tctctgatct ctttcctgaa gtctttatat ttaatgtcaa aatccatagt tagcctttca
     1861 gtagattgcg ggaaatcaca attctctgag cctcagaagt accctcataa atttcggtga
     1921 cacgagcatc acggaaatag cgctcgaccg ggtattcctt gacataacca tagccgccat
     1981 gaatctgtac ggcctcatca acgacataat tggacatctc ggttgcgaac agcttggcca
     2041 tagagatctc ccggctgtgc aaaccctcgg catcgttaag aaccgcggcc cggtaaacca
     2101 gcaatttagc ggcgtcgatc tgggtcgcca tatcagccag cttgaactgt atggcctgaa
     2161 attcggagat cggccggccg aactgctttc tctccttgga ataattgaat gcttcacgga
     2221 aagccgactc ggcaattccc agcgcctgcg ccgcgacacc gatacgccca ttatccagaa
     2281 gcgacaaggc caccttaaag ccatgattct cctcgccaat caggttggcc ttcggtactt
     2341 tggcatcaat gaatgaaact tcgcgtgtat ccgaacactt aagccccatc ttcttctccg
     2401 gcgcacccag cgacatcccc tcagttccgg ccgggactat gatagccgag attcccttgg
     2461 gacctttctc cgggtcagta agtgcaaaga ctataattat gccggcccgc tgggcggagg
     2521 tcacccagct cttggtgccg ttaaaaacgt aatgatcacc ttcggcaatg gcctggcatt
     2581 tcaaacttcc ggcgtcggta ccggaacccg gctcggaaag cgaatatgcg ccgattatct
     2641 cgcccgatgc gagcttcggc agatattctt tcttctgttc atcggttccg aaatactcga
     2701 tcgccttcat gaccagcgaa ttgtgtacac tgatcatgat cgcaaatccg gcgcaggcct
     2761 tggaaagttc ttcgatgatc agagcatagc tcaaggtatc gataccctca ccgccatatt
     2821 cctcgggagc cagaaggccg aagaatccga gttccttggc ttcctcgata acctcgtcgg
     2881 gcatatactc cttggcatcc atctcctcgg cgatgtcaaa cagcctcttg gcggcgaatt
     2941 cacgtccgaa atcgcgcatc ataatgtgat cttcagataa atcaaaattc ataggaactc
     3001 cttctatttg gagtagtcat aaaatccacg tccggtttta cggcccagat aaccggcctc
     3061 aaccatcttt gtcaataacg ggcagggggc gtacttggga tcgccaaatc cctcatgcat
     3121 aacctcgaga atcgcgagac agacatcgag gcctataaag tcggccagtg tcagggggcc
     3181 catcggatga gccatgccca gcttcatgac cgtatcaata gcttcctcgg tggcaacacc
     3241 ctcaaataac gcccagacag cctcgttaat catcggcagc agaatcctgt tggccacgaa
     3301 gccggggtaa tcattaacct cgaccggagt cttgcccagc ttcttggcta attcctcgac
     3361 cgtccggtag gtatcatccg aagtcgccac accgcgaatc acctcaacca gcttcatcat
     3421 cggaaccgga ttcataaaat gcatgccgat aacacggtcc gctctcccgg ttgcgctggc
     3481 gagctttgtg attggcagag aggatgtgtt cgaggcgatt attacctcgg gggccgctat
     3541 gctatctatt tttttaaaca gtttcagctt ggggtcgagt tcctcgataa tcgcttcgat
     3601 cacgagctga gagccctcgg catccttttc attcgtggag gtagttatcc gggtcaacga
     3661 ctctttcatc tgatcctcgg ttataacctc ttttttgacc tggcgctgca tgttcttctc
     3721 gatcgtcgat atggccttgt cgagaaactc ctgcttgata tctattagcg ttactttata
     3781 tccattctgt gcgaatacat gagcgatacc gttacccatc gtcccgccgc cgaaaactgc
     3841 tacattgcta atttccaatc taatcctccc ttacttttgt ttcattaccg gaaatcgtta
     3901 taccatctca attgaaagtg cgaccgcgct tcctccgccc aggcaaaggg tggcaatacc
     3961 ggtctttttg ccgcgatcct ttaacgcatg catgagtgtt accagtacac gagcgcccga
     4021 cgctccaatc gggtgcccca gtgcgaccgc gccgccatga acattgacac gatcccagtc
     4081 ccatccaagc tctttgccgt cggctaatgc ctgcgcgctg aaggcctcgt tggcttcgat
     4141 caggtcgaag tagttgatat ccttctgatc cagcttcttc atgacttttc tcaccgcata
     4201 tataggtgtg aagaagagat ccttgggatc gagactggca gaatcatagg cggtgatctt
     4261 ggcaatcggt tttaagccgt gtttgtctat gtattctttg gaacagacga ccactgccgc
     4321 ggaaccgtca ttcagccccg gagcattacc tgctgtgact gtgccatcct tcacgaatgc
     4381 cggtctcagt ttggcaaatt tttcaagcgg ggtgtccctg ggcccttcat ccttattgaa
     4441 aacaaccgga tcgccttttc tctggggaat ctcgatcggg aaaacttcct catcaaattt
     4501 gccgtcggcc catgccttca gagctttttg atgggaatta taagcgaatt catcctgctc
     4561 ctcacgggta attcccgcct tgtcagcagt gtattcggcc gagttgccca tatggtggtt
     4621 gttgaacgag tcccagaggc cgtcataaac catgatatca agcagtttct ggtcgccgaa
     4681 tttaaaaccg cgtttggcag cagccgggag aacgttggga gcattggaca tactctccat
     4741 gccgccgcag acaaagcagt gaccgtcccc ggccttgatc tgcgaggcgg ccatcatgac
     4801 agccttcagg cccgagccgc aaaccttgtt tattgtgatc gccggcactt cgggcggtat
     4861 gccgccgtga attgcggcct gacggccggg agcctggcct gagccgccct gtaccacctg
     4921 gcccatgatg acctcatcaa ttgtggcggg atcgatattt gcccttttta cgacttcttt
     4981 aatcgcaaat gcgcccaact gtggcgctgt aaatgctgat aagtgactgt gcagaacacc
     5041 gatagcggaa cggcaaccag ccacaatata agcttcagga taatccatta aatcctccgt
     5101 gagtgaatat tcttataata agcgtgactc atttttatca ggttatgcct ccttcagttc
     5161 ggagaggcag agatcgtgaa acaattctca attataataa aataacccct tttcggcaat
     5221 agaattcgca gaaaagctaa agttgatttt agggtggatt tgcgggtttt gaagcaggtt
     5281 ttgcggtgcc taccgcacgc gtcattgcga gttcggccct ggccggacgt ggcaatctca
     5341 aagccttaat taataatgat aactgagatt cccacgtcga tcataatcca ggattatgat
     5401 ctcctcggaa tgacgagatt aggggcagct tgctctgact tgcttgggaa ggtaattggc
     5461 tgtaatccag cacgactaat gcgagtccca gattcggcta ttcacggatt tgggcgtgcc
     5521 aatcgccggg ttggggaacc cgatggcaca tatcgattca tacccc
//
LOCUS       DSEB01000081            2875 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004516, whole genome shotgun sequence.
ACCESSION   DSEB01000081 DSEB01000000
VERSION     DSEB01000081.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004516"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            895..>2875
                     /locus_tag="ENO07_01545"
     CDS             895..>2875
                     /locus_tag="ENO07_01545"
                     /inference="COORDINATES: protein motif:HMM:PF00082.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HDS00679.1"
                     /translation="MSRAATCILLALMFVIGLSSSLLGDADFVPRPLGANEKVVPGML
                     IVQFKEYALPQHQKVFQGDQLSLGNTSIDALNQKYGAYKAEQVFAGMEINEKNADLNL
                     PNFYAIFFDPDMDVMEAVFEYGKNEYAVLAHPNHIYELRDMSNDPMLPDQYWLHQTSD
                     RDIDAPEAWSYEPGSDEIIVAINDTGVLWNHPDLWKNIWQNLGEDADGDGRTIQGNAF
                     DPGDINGIDDDGNGWVDDFIGYDWCNVYSSWVCPGEDYGGYDNDPMDFAGHGTNIAGL
                     ISATTNNGVGVSGVAGGFYPNFPGVRIMCLRSGYLSDDCETGSVDSYAGVQAMNYARL
                     NGADVINISFGPPYTPPCSPGYGLNTAMRSAIINCLNEDIVVTVAAGNDAVDCPDYMA
                     LIPGVINVAALESDDSRAWFSNYGDWIHIAAAGVDMQTTEGANGVPSYAAWNGTSFSA
                     PVVAGVAALVRSHDPLLSNDSILNLLMNTADPVSDPNVGAGRVNANNAITALPTAGFT
                     SASELDTFPPFNVSFTDISPTSGITDWAWDFGDGGTSDVQNPTHEYTEAGIYDVSLTI
                     TAPSGEDTYRYPKMVFAVGDTLWIDQIEELSVGDTADVYFNLKNTESLNEIKVPFIND
                     ADLSWVDYTIYTEGTRCEYFEKASIVGFGSNRAYIH"
BASE COUNT          703 a          659 c          754 g          759 t
ORIGIN      
        1 gatattcccc gtttgaggtt gatttctgga aatattactt cggggacgga gattcaagcg
       61 atttgcagaa tcccgtccat acttatatgg agccgggagc ctacaccgta acccaggaaa
      121 tttcgaccgc aaacggtgat gcctcaaata cgctggacag cttcattttt gtcctcgcgg
      181 acacattgac cggagttgat tttgagggag agattaccac aaccgaggtt atggtgcctt
      241 tcaggctaaa aaatatcgcg ccaatcgatg aaatgatact tccgattacc tatgacggac
      301 ctctggatct ggtttttgat tcgttcagtg ttgctggaac ccgcactgaa tattttgaaa
      361 atacgcaaat tgtggctttg aatttctctc aaaaagtgat tgtattcaga ttacgggcca
      421 atagcggagg cggggcgccc cctctttcag ccggtgaggg tgacattctg agactttatt
      481 ttcatgttgg aacggcaaca ccggatacaa ccgttattga caccctgact tttaacaatt
      541 atgtgcttga tttttcgacg tttattggcg attatatacc taggtacagt gccgcggaga
      601 tatctctctc gagcggtatt cgcggagatg caaatggcga tcaggtgctg aatgtctccg
      661 atgctgtgca tataatcaat tttgtatttt tgaacggagc gcctccggta acgcagtgcg
      721 gcggagatgc caataatgat gcgcagatga atgtcagcga tgctgtgtat atcatcaatt
      781 atgtattcgt caacggctcc gctccggatc cttgctcctg atacttcgac ctaaccgcct
      841 gcattatgat tggttgaaag aaaagcatat tttaaaatta taaaggagtc agagatgtca
      901 agagccgcaa cctgcatcct ccttgcttta atgtttgtga ttggtctgtc atcaagtctt
      961 ctcggcgatg cggattttgt gcccagacct ctgggcgcga atgagaaagt agttcccggg
     1021 atgctgatcg ttcaatttaa ggagtatgcc cttccgcagc atcaaaaagt ctttcaggga
     1081 gaccagctgt ctctcggaaa tacctcgata gatgcattaa atcaaaaata cggcgcctac
     1141 aaagctgagc aggtttttgc aggcatggag attaacgaga aaaatgctga tctgaatctg
     1201 cccaattttt acgccatatt tttcgatccg gatatggatg tgatggaggc ggtgtttgaa
     1261 tacggcaaaa acgaatatgc cgtgctcgct catcccaacc atatctatga gctgagagat
     1321 atgtcaaatg atcccatgct gccggaccaa tactggctgc atcagactag cgacagggat
     1381 atagatgcgc cggaggcctg gtcgtatgaa ccgggcagcg atgagatcat tgtggccata
     1441 aacgacaccg gcgtgctatg gaaccatcca gatctgtgga aaaatatctg gcagaatctg
     1501 ggtgaggatg cggatgggga cggccgcacg attcagggca acgcatttga cccgggtgac
     1561 atcaacggaa tcgatgatga tggaaatggc tgggtggatg attttatcgg gtatgattgg
     1621 tgcaatgtct actcgagctg ggtttgtccc ggcgaagatt atggcggata tgataatgat
     1681 cccatggatt tcgccggtca tggaacgaat atcgccggcc tgatttcggc cacgacgaat
     1741 aacggggtgg gggtatctgg tgtggccgga ggattttacc cgaatttccc cggtgtcaga
     1801 attatgtgcc tcaggtcggg ctacctttct gacgactgcg aaaccggcag tgttgacagt
     1861 tatgccggtg tgcaggcgat gaactatgcc cgcttaaatg gcgcggatgt aataaatatc
     1921 agttttggtc ccccgtatac gcccccgtgt tcgcctggat atggcttgaa taccgccatg
     1981 cgcagcgcta ttataaactg tctcaatgag gatatcgttg tgactgttgc agccggaaat
     2041 gatgctgttg actgccctga ttatatggcc ctcataccgg gagtgatcaa tgtggccgct
     2101 cttgaaagcg atgacagccg ggcctggttc tcgaattacg gggactggat tcatattgcg
     2161 gccgcaggcg tggatatgca gacgaccgaa ggagctaacg gcgttcccag ctatgccgcc
     2221 tggaacggca cttctttctc cgccccggtt gtggcgggag tggcagcgct ggtgagatcg
     2281 catgatcccc tcctcagcaa tgattcgata ttgaatctgc ttatgaatac cgccgatccc
     2341 gtttcggatc ctaatgttgg tgcagggcga gtgaatgcaa ataatgctat tacagcgctt
     2401 cccaccgcag gcttcacgtc tgcctccgag ctggacacat ttccgccttt caatgtgtcc
     2461 tttacagata tctctcctac ctcgggaatc accgactggg cctgggattt tggtgacggc
     2521 ggcacctctg atgttcaaaa tcccactcat gaatacactg aagcggggat ttatgatgtc
     2581 tccctgacaa taaccgcccc ctcaggagag gacacataca ggtatcccaa aatggtcttt
     2641 gcggttggcg acactctctg gatcgaccag attgaggagc tttcagtggg cgatacggcc
     2701 gatgtttatt tcaatctgaa gaatacagaa agcctcaacg aaataaaggt acccttcata
     2761 aatgatgccg atttgagctg ggtcgattat acaatttata ctgagggcac gcgctgtgaa
     2821 tattttgaga aggccagcat cgtcggtttc ggctctaaca gggcctatat acatc
//
LOCUS       DSEB01000082            4420 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001742, whole genome shotgun sequence.
ACCESSION   DSEB01000082 DSEB01000000
VERSION     DSEB01000082.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001742"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..325)
                     /locus_tag="ENO07_01550"
     CDS             complement(<1..325)
                     /locus_tag="ENO07_01550"
                     /inference="COORDINATES: protein motif:HMM:PF00675.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDS00680.1"
                     /translation="MKRAALFLQIIILLPAVLTAQISLDIEKTVLDNGLTILTYEDHS
                     FPTVSYQTFVNAGSRDELRPGATGIAHVFEHMMFRGTPSHPDYDDAVAHMGPQTNAYT
                     GQDYTC"
     gene            390..1928
                     /locus_tag="ENO07_01555"
     CDS             390..1928
                     /locus_tag="ENO07_01555"
                     /inference="COORDINATES: protein
                     motif:HMM:PF13181.4,HMM:PF13472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDS00681.1"
                     /translation="MKSLAAQLKLLYDMEMTDKKIDQKISLSRKISYGIIAAVLVLII
                     LEITLRLAGLFPARKLFEKSQSSASWQENLFAGFMGIHEPAPELLWKMKPNLNKTFVQ
                     TNSRGLTGRLIPYEKDTAKLRILVLGDSTPLGIGLKDWDNSYVWLLQEFLERQLGREV
                     EIVNASTAGYTSRQGLEYLREEGLKYDPDFVLVYLGNNDASYNGYLSDSALMAQASEY
                     IGLKKVLNNFKIYRMLKSILIPLKSGTKDYEGLELQVRVPPHRFENNLAEIIEISRKN
                     GAGVILNTIPVPLTWPPGVEFKVFTTGRDTVSGQLFMPQKQREILEQKMSLALDWEMF
                     RQQYGQIDPWSQRVLQSAYTDSGSIVENIAGYRRLLEGDSSNSGYLNNIGVLYWQSEE
                     YDSARIYLKKALSYDPADPSVMYNMGMTFLKQGMTDSADHYLRMARDYDYNSLRIKSD
                     YNEIIENISQNKGIPLIDLKADFERLGREKLFVDHCHPNQIGHRIIAEQLTDKILEII
                     NSQN"
     gene            1938..2942
                     /locus_tag="ENO07_01560"
     CDS             1938..2942
                     /locus_tag="ENO07_01560"
                     /inference="COORDINATES: protein motif:HMM:PF01926.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HDS00682.1"
                     /translation="MPANLTPQYKEAEERFRNASTDEERLACLEEMMRVIPKHKGTEK
                     MRADIKTRISKIKQKLESGKKSRGGGARRTSYLDHVEKHGAAQIIILGPPNSGKSSLV
                     KLVTNTEPEIADYPFTTRTPMPAMMPFKTIQFQLVDMPPISRETYDGWMTNLIRNGDM
                     IVIVVDVSADDLLESLDETLEVIRDAGIVLTGETIPEEYQNSVFCKKTLILANKIGEE
                     NAPDIFQILQEFYGDKFPILGISVLRNRNIEQFKRRIFEMLKIIRVYTKSPGKEVDYK
                     DPIILPIGATVEDAAVSIHKDFAEKLQYAKVWGEGKFDGQRVTNSFQLTDGDIIEFHI
                     "
     gene            3052..>4420
                     /locus_tag="ENO07_01565"
     CDS             3052..>4420
                     /locus_tag="ENO07_01565"
                     /inference="COORDINATES: protein motif:HMM:PF00675.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDS00683.1"
                     /translation="MITKKIIIYSLFLILSVTSLVHAASDDGTTYANLGNGMKLVLKE
                     NHSSPMITSVVFVNAGARYENEKTNGMTHFLEHLLFNGTSTRDRIELNETIKEHGGYI
                     NAFTRKDLTAYLVLMPKEYIDTGLAIQADQLFNSALPEDEFPKEKKIVIEEIRKSNDV
                     PSTLADYYFNEVVFEGTPYARTVLGTAEHISSLTRADVIEYYKKYYIPNNMTALIIGD
                     FETERMIETFDRYFGHYEKGKMPKIDLVNFMIPDGRRIEETAIDTKNFHVDLALPAPH
                     YTDPDYYPYYMLVEYLGSGESSPLTKHIEDKGLASSIYSSLETQRDFSMLRISATTDS
                     EKNARAVIWEVEKILSNPEFLTPSYDAFRGMIVSKKTQEIYLREKLHYYGFIIAPMMV
                     STGYEFVETLVDSLENVEQAEMAEVASKYFSDIKYVGTLVKPAEFEEDEVLSAGITET
                     KKVTFD"
BASE COUNT         1268 a          967 c         1091 g         1094 t
ORIGIN      
        1 agcaggtata atcctggccg gtgtaagcat ttgtctgagg gcccatgtgc gccacggcgt
       61 cgtcataatc cggatggctt ggtgtgccgc gaaacatcat gtgttcgaag acatgggcaa
      121 ttcccgtggc ccccggacga agctcatcgc gtgagccggc gtttacaaat gtctggtatg
      181 ataccgtggg aaaggagtgg tcctcatagg tcaggattgt caatccgtta tcaagcacag
      241 ttttttcaat atccaggctt atttgtgccg ttaaaactgc aggtaaaagt atgataatct
      301 gcaaaaatag agctgctctc ttcattgaac ctcctgaatt tcaattagta taatacaagt
      361 gagccttcaa aagcaagcat aaagagtgct tgaaatctct ggcggcacag cttaaattat
      421 tatacgatat ggaaatgaca gataaaaaga tagatcagaa gatttcgctt tccagaaaaa
      481 tcagctatgg tataatagcg gcggtgcttg tgctaattat cctggagatc acccttcggc
      541 ttgcaggcct gtttcccgcc cggaagctct ttgaaaaaag ccagtcatcg gccagctggc
      601 aggagaatct ttttgccggg tttatgggta ttcatgagcc cgctccggag ctgttatgga
      661 agatgaagcc gaacctgaac aaaacttttg tgcagacaaa ttcccgaggt ctaaccgggc
      721 ggctcattcc ctatgaaaag gataccgcaa aattgagaat cctggttctg ggcgactcca
      781 ctccccttgg aatcggcctc aaagactggg ataattcata tgtttggctt ctgcaggaat
      841 ttctggaaag gcagcttggc agggaagttg aaatagtgaa tgcctccacc gccggataca
      901 cctcgcggca gggattggaa tacctgcgtg aagagggctt gaaatacgat ccggattttg
      961 tacttgttta tctcgggaat aatgacgcct cctataacgg ctacctttca gactcggctt
     1021 tgatggctca ggcttcggaa tatattggcc tcaagaaagt acttaataat tttaagattt
     1081 accggatgct taagagcatt ttaattccgc ttaagtccgg aacgaaggat tatgaaggat
     1141 tggaattgca ggtgcgggtg ccaccgcaca ggtttgaaaa taatcttgcg gagattattg
     1201 aaataagcag gaaaaacggc gccggtgtca tattgaacac tataccggtt ccgctgacat
     1261 ggccgcccgg cgtggaattt aaggtcttca cgaccggacg ggacacggtc agcggtcagc
     1321 tttttatgcc gcagaagcag cgcgagatac tggaacagaa gatgtctctg gcattggact
     1381 gggagatgtt tcgacagcaa tatggccaga tcgatccctg gtcgcagcgc gtacttcaat
     1441 ccgcctatac agactctggc agtattgtcg aaaatattgc cggttatcgt agattgctcg
     1501 aaggtgacag ttcaaattca ggatatttga ataatatcgg ggtcttgtac tggcagagcg
     1561 aggaatatga cagcgcccgt atatatttga aaaaagcgct atcctatgat cctgcggatc
     1621 catccgtcat gtacaatatg ggaatgacct tcttgaaaca gggcatgacc gattccgcag
     1681 atcattatct gcggatggcg cgggattatg actataattc actccggatc aagagcgatt
     1741 ataacgaaat tattgaaaat atttcgcaaa ataagggtat tccattaatt gatctgaagg
     1801 ctgatttcga gcgtctgggg cgtgaaaaat tgtttgtgga ccactgccac ccaaaccaga
     1861 tcggccatcg gatcatagcc gagcagctga cggataaaat cctggaaatc ataaacagtc
     1921 aaaattaata tcatattatg cctgcgaatt taacaccgca atataaggag gcggaagaga
     1981 gatttcgcaa cgcctctacc gatgaagagc ggctggcctg cctggaggag atgatgcggg
     2041 tcatccccaa acataagggc accgagaaga tgcgggcgga tatcaagacc cgtatatcca
     2101 agatcaagca gaagctggaa tcggggaaaa aatccagggg cggaggagcc aggagaacat
     2161 cctatctcga tcatgtagaa aagcatggcg cggcccagat aataattttg ggaccaccta
     2221 attcagggaa gtcatcgctg gttaagctgg ttacgaacac cgagccggag attgcagatt
     2281 atcccttcac caccagaacg cctatgccgg ccatgatgcc gttcaaaacg atccaattcc
     2341 agcttgtgga tatgcccccc atcagccgcg agacatatga cggctggatg acgaatttga
     2401 tccgcaacgg cgatatgatc gtgatcgttg tggacgtctc cgccgatgat ctgctggaaa
     2461 gcctggatga gactctggag gttataagag atgccggtat tgtgcttaca ggtgaaacga
     2521 ttccggagga atatcaaaat tctgtgttct gcaagaagac gttgatcctc gccaacaaaa
     2581 tcggggagga aaatgccccg gatatctttc aaatcctgca ggagttctac ggcgataaat
     2641 ttccgattct cggcatctcc gtgctgcgca accgcaatat cgaacagttt aagcgccgta
     2701 tatttgagat gttgaagata atcagggttt ataccaaatc cccgggcaag gaagtggact
     2761 acaaagatcc tattatattg cccatcgggg caacggttga ggatgcggcc gtcagtattc
     2821 acaaggactt tgccgagaaa ctgcaatatg ccaaggtctg gggcgagggc aagttcgacg
     2881 gccagcgggt aacaaattca tttcagctca cggatggtga tattatcgaa tttcatatct
     2941 gaataatatc tctgggtgac ttgaacagaa aacataaaag acttgacaga gataggcagt
     3001 tagatttatt gctgaagttt gcatcaaaac tttatcaaag ggagaattaa aatgatcaca
     3061 aagaaaatca taatatattc attattcctg attctatccg tcaccagcct cgtacatgcc
     3121 gcaagtgatg acgggacaac ttatgccaat ctcggcaacg gcatgaagct tgttctgaag
     3181 gaaaatcatt catcgcccat gataacatcg gttgtctttg tcaatgccgg ggcgcgttat
     3241 gagaatgaaa aaaccaacgg catgacccat tttctcgaac atctgctctt taacggcaca
     3301 tcaactcgtg acagaatcga actgaacgag actatcaagg agcacggagg ctatataaat
     3361 gcgtttacgc gtaaagatct gaccgcttac ctggttctga tgccgaagga gtatatcgat
     3421 actggactgg caattcaagc ggaccagctt ttcaattcgg ccttgcccga ggatgaattt
     3481 cccaaagaga agaaaattgt tatagaggaa atccgaaaat caaatgatgt ccccagcacg
     3541 ctggcagatt attacttcaa tgaagttgtt ttcgagggca cgccttatgc acgcacagtt
     3601 ctgggtaccg ccgagcatat ttcatctctg acccgtgcgg atgttataga atactataaa
     3661 aaatattata tccccaataa tatgaccgcg ctcataatcg gggatttcga gaccgagagg
     3721 atgattgaaa cttttgacag gtattttggc cattatgaga agggcaaaat gccaaagatc
     3781 gatcttgtca atttcatgat tcccgatgga cgccggatcg aggagaccgc gatcgacaca
     3841 aagaactttc atgttgacct ggctctaccc gccccccatt acaccgatcc cgattattat
     3901 ccctattata tgctggttga gtatctcggc tcgggagaaa gttcacccct gacaaagcat
     3961 atcgaggaca agggcctggc ttcttcaata tactcatccc tggagaccca gagagacttc
     4021 tccatgctaa ggatttccgc tacgaccgat tcagagaaaa atgccagggc ggtaatctgg
     4081 gaagtcgaga agatactctc aaatcccgag ttcctgacgc catcctatga tgcttttcgg
     4141 gggatgatcg tgtccaaaaa gacgcaggaa atttacctgc gtgaaaaact gcattattat
     4201 ggatttatta tcgcacccat gatggtttcg accggctatg agtttgtgga aacattggtg
     4261 gacagcctgg aaaatgtgga gcaggctgaa atggccgagg tagccagtaa atatttttcg
     4321 gacatcaaat atgtcgggac acttgtaaag cctgcagaat ttgaagagga cgaggttctt
     4381 tcagcaggca tcaccgaaac taaaaaagtc acatttgata
//
LOCUS       DSEB01000083            2201 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1008192, whole genome shotgun sequence.
ACCESSION   DSEB01000083 DSEB01000000
VERSION     DSEB01000083.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1008192"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            160..1215
                     /locus_tag="ENO07_01570"
     CDS             160..1215
                     /locus_tag="ENO07_01570"
                     /inference="COORDINATES: protein motif:HMM:PF13439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDS00684.1"
                     /translation="MRVALVASARSWHTYRWYKSLRTKCDIRVFTFNRGTDSYGKDQI
                     VFESRLPQKLRYILGSKKVKRSVCSFQPDILHAHYATGHGYLGYKMGLHPFVVSVWGS
                     DIFEFPHKNRYSRNLVKNVLESADAICATSQKLHDGTLSLYPEFEAKIKVIPFGIDMN
                     LFRPENSKKAEGKITIGTAKILREIYQIDMLMKVFDRIADDYPNLSLRIAGDGPQEGE
                     LFELMKSLRHGDQIEFVGRIENDRMPEFLNQLDIFVLPSKFESFGVSALEASACQLPV
                     VAFDVGGLSEILHDGKSAFLVSGGDIKGFEDAIRALVEDEKKRLEMGLAGREIVFNKY
                     DVEKTTRIQLELYKSLT"
     gene            1302..>2201
                     /locus_tag="ENO07_01575"
     CDS             1302..>2201
                     /locus_tag="ENO07_01575"
                     /inference="COORDINATES: protein motif:HMM:PF01041.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HDS00685.1"
                     /translation="MKIESVPFFRMKFTRSEIDAVTRTIKSGWLTTGNISHQLEDKFC
                     RYVEARYGAAVGSCTAGLHLALKVLGLSRGDEVITTPYTFVASTEAILHCGARPVFAD
                     IDPVTLNIDPNRIEEKITPRTRAVVPVHIAGLPCRMEIITRLARKHKLLIVNDSAHAI
                     GAEFKGKKIGAYGDLSSFSFYATKNLTTAEGGIVTTNSRKLADEIRVLSLHGMDKKAW
                     KRYLDQGSWYYEVVSLGYKYNLADMNAALGLAMLDRLDILQQKRERIASWYDQALVKF
                     DEIELPARERDSKHAWHLYIIKLN"
BASE COUNT          602 a          467 c          562 g          570 t
ORIGIN      
        1 atcaaattcc atcgcctatt ttcttacatt tttgagctta ttgattataa taagatttaa
       61 atttcgacag agactgctgc tgttcttcat gatcaggagg caggacttga gagagatttc
      121 agcaaacata agatcattcc ttggcaggaa agcaagtcaa tgagagtggc ccttgtcgca
      181 tcagccagaa gctggcatac atacaggtgg tacaagtcgt tgcggacaaa atgtgatatc
      241 agggtgttta cttttaaccg gggcaccgat tcgtatggca aagaccagat agtctttgaa
      301 tcccgcctgc ctcaaaaact gaggtatatc ctggggtcga aaaaagtgaa gaggtcagtt
      361 tgttcttttc aaccggatat tctccatgct cattacgcca cagggcatgg ctatctcggt
      421 tacaaaatgg gattacatcc ctttgtggta tcagtctggg gctcggacat ttttgagttc
      481 ccgcataaaa acaggtattc aagaaatctg gtcaaaaatg tactcgagag cgccgatgca
      541 atctgcgcta ccagccagaa actgcatgac ggtactcttt cactttatcc tgaattcgaa
      601 gcgaagataa aggttattcc ttttggcatt gatatgaatc tttttcgccc ggagaactcg
      661 aaaaaagcag agggtaaaat aacaatcggc acggccaaga ttctcaggga gatttatcag
      721 attgatatgt tgatgaaagt ttttgacagg atagcggacg actaccccaa tctgagttta
      781 aggatagcag gagacggccc ccaggaaggt gagctgtttg aattgatgaa aagcctcagg
      841 catggtgatc agatcgaatt tgttggcagg attgaaaatg atcgcatgcc tgaatttttg
      901 aaccagctgg atatatttgt gctgccctca aaattcgaga gttttggtgt ttcagctttg
      961 gaagcctcgg cctgccagct gccggttgtg gcttttgatg tgggggggct ttccgaaatt
     1021 ttacatgacg ggaaaagcgc atttctggtt tcgggcggcg atataaaggg attcgaggac
     1081 gccattcgtg cccttgttga ggacgaaaaa aaacggttgg agatggggct tgccggcagg
     1141 gagattgtgt ttaacaaata tgatgtggaa aagacaactc gaatacagct cgaattatac
     1201 aaatcgctta cttgaaaagc agggtggaat tctataaaaa tattgtggat acgctcttta
     1261 ttttttacat ttcctgattc tcagattacc ggaaggagca tatgaaaata gaatccgtac
     1321 cgttcttcag aatgaaattc acccggtccg agatcgatgc tgttacccgg acgatcaaaa
     1381 gcggatggct taccaccggt aatatctctc atcagctgga ggacaagttc tgccgctatg
     1441 ttgaggccag atacggcgcg gcagttggat cgtgcactgc gggccttcac ctggcgctca
     1501 aagtgctcgg gctctccagg ggggatgagg tgattaccac tccatataca ttcgtcgcct
     1561 cgaccgaggc tatcctgcat tgcggcgccc ggcctgtttt tgcagatatc gatccggtca
     1621 cgctgaatat cgatccgaac aggatagaag agaaaataac gcccagaacc agggctgtcg
     1681 taccggttca cattgccggg ttgccctgcc gtatggagat aattacaagg ctggccagaa
     1741 agcacaagct ccttatcgta aatgattcag cccatgccat tggcgctgaa ttcaagggca
     1801 aaaaaatcgg cgcttacggt gacctttcca gcttctcttt ttatgccacc aaaaatctaa
     1861 ccaccgctga aggaggcata gtaaccacaa atagcaggaa gctggcggat gaaatacgcg
     1921 tgctttccct gcatgggatg gacaagaagg cctggaaacg ctatctggat cagggaagct
     1981 ggtattatga ggtggtctcg ctgggatata agtacaatct tgctgatatg aatgccgccc
     2041 tgggcctggc catgctggac cgtcttgata tactccagca gaagagagag aggattgcct
     2101 catggtatga tcaggcattg gttaaattcg atgaaattga acttccggcg cgcgagaggg
     2161 actcaaagca tgcctggcat ctttatataa taaagctcaa t
//
LOCUS       DSEB01000084            3727 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002511, whole genome shotgun sequence.
ACCESSION   DSEB01000084 DSEB01000000
VERSION     DSEB01000084.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002511"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..338)
                     /locus_tag="ENO07_01580"
     CDS             complement(<1..338)
                     /locus_tag="ENO07_01580"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_953068.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDS00686.1"
                     /translation="MFKKILIANRGEIALRIIRACKEMGVKTVAVYSEADLDSLHVRF
                     ADEEVCIGPPPSNQSYLDVKRIISAAEVTNCDAIHPGYGFLAENADFAEICESCGLTF
                     IGPSPESMRM"
     gene            complement(1230..1979)
                     /locus_tag="ENO07_01585"
     CDS             complement(1230..1979)
                     /locus_tag="ENO07_01585"
                     /inference="COORDINATES: protein motif:HMM:PF09986.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HDS00687.1"
                     /translation="MNKWSRAVKLFLTIDILSILFVFLGSCAFATTTYEVEVECSVCG
                     NKVKQRKISSTNTFGSMDLDTRPPEMQRSTMMYWVNCCPDCGYCAGSVDEELENAREL
                     IGTEEYKTIRADTLYPPLARDFLSHAFLLEKNEMYKEAAWACLHAAWDCDDWGGNSVN
                     CRLNAARLFKMVKPDESETPTDRENRLTLLVDILRRARQFDEALVVCDSALQLAETKI
                     IVDILNFQKTLILNKDDGRYVVEQAVSEEKE"
     gene            2431..2619
                     /locus_tag="ENO07_01590"
     CDS             2431..2619
                     /locus_tag="ENO07_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00688.1"
                     /translation="MNTKSKNPALGNGIYHPGARPMVVYSDDEGCMWLCDKGTDPERG
                     LHEQGCWRCRDLAFTRND"
     gene            2826..3242
                     /locus_tag="ENO07_01595"
     CDS             2826..3242
                     /locus_tag="ENO07_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HDS00689.1"
                     /translation="MKVKIRQIDGIALAAAGDTNHWVTMDGPEDFGGHSAGSRPMELL
                     LMSLGSCTAMDVLSILQKKRVKLDDFQMEVESERAEEHPKVFTEIKLHFIFVGKGIRQ
                     KDVERAIELSTDKYCPATAMLKKSVKITNDYKIIEG"
     gene            3257..>3727
                     /locus_tag="ENO07_01600"
     CDS             3257..>3727
                     /locus_tag="ENO07_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDS00690.1"
                     /translation="MPGSQPFDKYAEQYDDWFEQNHIAYETELKAVKHFLPDSGTGLE
                     TGVGTGRFAGPLGIKNGLEPSKSMRAIAKERGIDAVDGRAEKLPYNDGEFDYVLMVTV
                     ICFLDDVRIALDEARRVLNDGGIIVIGFIDRESPLGQMYEKHKNENEFYKHAVFY"
BASE COUNT         1018 a          871 c          884 g          954 t
ORIGIN      
        1 atcatgcgca tcgattccgg cgagggcccg atgaaggtca ggccgcagga ctcacagatt
       61 tccgcaaaat cggcattttc cgccaggaag ccgtagccgg gatgtattgc gtcgcaatta
      121 gttacctcgg cggctgaaat tattcgttta acgtccagat aggactggtt ggagggtggt
      181 gggccgatac agacctcttc gtcggcaaag cgcacatgca ggctgtccag gtcggcctcg
      241 gagtagaccg ctacggtctt gacgcccatc tccttgcagg cccttataat cctgagtgct
      301 atttcgcctc tgttggctat aagtattttc ttaaacatgg cggaaaagta taaatatcaa
      361 cactctttgg caagctaatt cttgggaaat gcaaggagtg tactattggt cgtcattgcg
      421 aggagcgaag cgacgtggca atctcacagt ttattccatg aaccaatctg tgagattccc
      481 actcctcacc ggccttcggc cgattcgggg atggggcacc cgcaatctca cagcctttgt
      541 agatcaagcc ccgtcaaagg tctggccggc agccttaagg ggcttgacga ggttattggg
      601 aaatggaatt gtcaattccc ttcaggaatt gacctacagt ctttgattcg tgccgggcgt
      661 acgtctcgtg cgcacagaaa tcgcagggca tgatccctgt gatcatgcca atccaaatca
      721 tcccgggtgc cgccgacaaa ctcgtttgtc ggcgatctct cgaatcaagc atccgccaca
      781 aatcaagttt gtggcgggca ccctaaattc agcgcagagc agaggatgta ctccgcgcac
      841 gataaatata cttatgtggg cgctaagttc cctaacctga cgccctaatc gcaggattgg
      901 gaatcccggc ggtacatgat aagaatttat gtcaaagaat cccacccctc gctggctctc
      961 agcctgttcg ggatggggca cctgagcgac ttttgacgga agtggtaatc tcatggttca
     1021 tctttatacc aaatatggag attcccacgt cgatcataat ccgagattat gatctcctcg
     1081 gaatgacgat tcaaataact tggatccctt caaaggatct gaatcctatc ataacgcaga
     1141 aatttatttc cggagaaatc gtccgtcaaa agtctctgac ttttgacagt gtcaaatcta
     1201 acagagattt gacctacggg gctgcctttc tattcttttt cctcgctgac agcctgctca
     1261 actacatagc ggccatcatc tttattaagt atcaaggtct tctgaaaatt caggatgtcc
     1321 acaatgatct ttgtctctgc aagctgcaaa gccgaatcgc acacaaccaa tgcctcatca
     1381 aactgtctgg cccttctcaa gatgtccaca agaagtgtca gtctgttttc tctatcagtc
     1441 ggcgtctcgg attcatctgg cttaaccatc ttgaacaacc ttgcggcatt cagacggcaa
     1501 ttcacactat tgccacccca gtcatcacaa tcccaggcag catgcagaca tgcccaggcc
     1561 gcctccttgt acatctcatt tttctccaac aggaatgcat gagaaagaaa atccctggcc
     1621 aaaggcgggt acagagtatc agcacgaatg gtcttatatt cctcggtccc tatcagctcc
     1681 cgggcatttt caagttcttc atccacactg cccgcacaat aaccgcaatc aggacagcag
     1741 ttgacccaat acatcatcgt tgacctttgc atctccggcg gacgggtatc cagatccatt
     1801 gacccgaaag tattagtcga tgatatcttc cgctgtttga ccttattccc gcacacagaa
     1861 cattcaacct cgacttcata agtagtggta gcaaatgcac atgaaccaag aaagacaaat
     1921 agaatgctca atatgtcgat agttagaaat aacttcacgg ccctactcca tttattcaga
     1981 tgagagtttt gtcttcagtc tgtttcccac attccaccct ttcaaatgag ggatgcgatc
     2041 atcataaata aaatagcaaa atagagcatc attgcaagca gtttatcctc aagacggcgg
     2101 aagaccgatt tcgttcagta aactcgccgt gactggtcaa aagtctgtgg cttttgacag
     2161 tgtcaaatct cacagagatt tgacctacga ggctggagtc gaaacttaat gtagtccgat
     2221 taggcttgca taaatgcatt aatgcaagtt tggaaattgc aatattgcat gatatgtttg
     2281 cagataaccg aagaatcgtt atctgactac tgcacgtaat ccctttatat ccatcaggtt
     2341 agatatatta tacacaatta ttgcatattt cggcacgatt tatgagatat aattggtttg
     2401 gaatggctct gaaatttaag gaggttcatt atgaatacaa agagcaaaaa tcccgcgctg
     2461 ggtaacggaa tctatcatcc gggcgcaagg ccaatggtgg tctattcgga tgatgaagga
     2521 tgcatgtggc tttgcgataa gggcactgat cccgaaagag gtctccacga gcagggctgc
     2581 tggagatgca gggatctggc tttcacacgc aacgattaaa gggcacgacc cgtaaggata
     2641 gcaatgtggt aaagggtttc aaggcttggc aatgaaacct gtgaacggtc gccatcattt
     2701 gatggcggct tgttcttaat tgccttaata acgtattttt tccctgcttc tttcataaaa
     2761 ttccatatta ttgacttggt ttagcattta taagtcataa ctcaattctt ggggagagat
     2821 gagagatgaa ggtcaaaata aggcagattg acggcatcgc gctggcggct gcgggagata
     2881 caaatcactg ggtaactatg gacggaccgg aagatttcgg ggggcactcg gctgggtcgc
     2941 gtcccatgga actgctcttg atgtcgctgg ggagctgtac agccatggat gtcctttcca
     3001 tcctgcagaa aaagcgggtc aagctcgatg attttcaaat ggaggtcgaa agcgagaggg
     3061 ccgaagaaca tcccaaagtg ttcaccgaaa tcaaattgca cttcatattc gtcggaaaag
     3121 ggatccgtca aaaagatgtt gaacgggcca tagagctgag cacagacaaa tattgtcctg
     3181 caactgctat gctgaaaaag agcgtgaaga tcacaaacga ttataaaatt atagaaggtt
     3241 aaacaggatt aatctcatgc ccggcagcca gccgtttgat aaatatgcag agcagtacga
     3301 tgactggttt gaacaaaacc acatcgctta tgagaccgaa ttgaaagcag taaagcattt
     3361 cctgcctgat agcgggaccg gtcttgagac cggggtcggc accggaaggt tcgcgggacc
     3421 tctgggcata aagaatggac tggaaccatc aaagagtatg cgggcaatcg ccaaagaacg
     3481 cggaatcgat gcagtcgatg gccgggccga gaaactgcca tataatgacg gtgaattcga
     3541 ttacgttttg atggtcacgg ttatatgctt tctcgatgat gttcgtatcg ctctggacga
     3601 agcccgacgt gtcctgaatg atggcggtat cattgttatt ggattcatcg atcgggaaag
     3661 cccgttgggt caaatgtatg agaaacataa aaatgaaaat gaattttata aacacgccgt
     3721 cttctac
//
LOCUS       DSEB01000085            1945 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1010716, whole genome shotgun sequence.
ACCESSION   DSEB01000085 DSEB01000000
VERSION     DSEB01000085.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1010716"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            242..574
                     /locus_tag="ENO07_01605"
     CDS             242..574
                     /locus_tag="ENO07_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_793148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HDS00691.1"
                     /translation="MYGLSYILGQRHTDHETNEPYESGIKTTESARIRFSAKFYLVAM
                     LFVIFDLEVVFIFAWAVGAVELGWPGYTALLVFLAILVAGLVYEWRMGALDWIGQSAG
                     KYRQRGDF"
     gene            625..1218
                     /locus_tag="ENO07_01610"
     CDS             625..1218
                     /locus_tag="ENO07_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HDS00692.1"
                     /translation="MADDSFQKSIQKSLVLSRLQDLIAWGRKNSMWPFSFGLSCCFVE
                     MATSLTSKFDIARFGAEVIRGTPREADVMIVAGTVFIKMAPIVRRLYDQMMEPRWVIS
                     MGSCANSGGMYDIYSVVQGVDKFMPVDIYVPGCPPRPEAFMEGLLLLRESVGREKRPL
                     SWVIGPQGVEKGKLPARRDLKREERRKIKDLRTPDQI"
     gene            1243..>1945
                     /locus_tag="ENO07_01615"
     CDS             1243..>1945
                     /locus_tag="ENO07_01615"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681341.1"
                     /note="NuoCD; NDH-1 shuttles electrons from NADH, via FMN
                     and iron-sulfur (Fe-S) centers, to quinones in the
                     respiratory chain; subunits NuoCD, E, F, and G constitute
                     the peripheral sector of the complex; in Escherichia coli
                     this gene encodes a fusion protein of NuoC and NuoD that
                     are found separate in other organisms; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HDS00693.1"
                     /translation="MKEPQIELDLIERFRDARIAPQRSKDGIPTFWVDKADVRSVLQY
                     LKDEAERPYRMLYDLTAIDERSRQNRNGQPDGAFTVVYHLLSVDRNEDIRIKTALDGK
                     YPSIDTITHMWHNANWYEREVYDMFGIKFDGHPNLRRILMPWTWEGHPLRKEHPARAT
                     EMGQFSLPQEKQDREQEAMRFKPEEWGMKRESEDTEFMFLNLGPQHPGTHGVLRLILQ
                     IDGEMIVDIVPDIGFH"
BASE COUNT          478 a          450 c          534 g          483 t
ORIGIN      
        1 agtttatacc ggtgccatca ataaaagtaa agggcttgaa aataatgcag cttccggttg
       61 ccatgagcaa attcacatta aaatgctgat gattttttga gtatttcgtt tccgcattca
      121 gtcatgtgaa tttgatcaca aaacaaaagg agtttgcagc ttggatgcgt tctggcatca
      181 gactctgaac atcctgcctt tcctggtata cactctggga gtcctgtttg tattagccgt
      241 catgtatggg ctgtcttata tcctggggca gcgtcacact gatcacgaga ccaatgagcc
      301 ctatgaatcg ggaataaaga ccaccgaatc cgcacgcata aggttttcag ccaaattcta
      361 tcttgttgca atgctgtttg taattttcga cctggaagtt gtattcatct tcgcctgggc
      421 cgtaggtgcg gtggagctgg gatggccggg ttatacagca ctgcttgtat ttctggccat
      481 actggtggca gggcttgttt atgagtggcg catgggcgcg ctggactgga taggccagtc
      541 tgcaggaaaa tatcggcaaa gaggtgattt ttagatatga acaagataca caaattatcg
      601 gactccggaa ttcatccgga cgaaatggct gatgattctt ttcagaaatc gattcaaaag
      661 agcttggtgc tctcgcgtct tcaggacctg atcgcatggg gacgcaaaaa ttccatgtgg
      721 ccgttttcat tcggcctgtc ctgctgtttt gtggagatgg ccaccagcct caccagtaag
      781 ttcgacatcg cccgtttcgg agcagaggtt atccggggca cacctcgtga agcggatgtt
      841 atgatagttg ccggaacggt ctttatcaag atggcgccca tagtgaggcg tttgtatgat
      901 cagatgatgg agccgagatg ggttatctct atgggttcct gcgccaattc cggcgggatg
      961 tatgatatct acagcgttgt acagggagtg gacaagttta tgcctgtgga tatctatgtc
     1021 cccggatgtc cgcccagacc tgaggctttc atggaagggc tgctgctcct gcgtgaatcg
     1081 gtcggcaggg aaaagcgccc cctgagctgg gtgatcggcc cgcagggagt cgagaaaggc
     1141 aagcttccgg cacggcggga tctaaagcgc gaagagagaa gaaagataaa ggatttacgt
     1201 acgcctgatc aaatttagct gacttttatg aatcaaaaga atatgaaaga gccgcagatt
     1261 gagctggatc tgattgaacg ttttcgggat gccagaattg ccccccagcg gagcaaagac
     1321 ggaattccca cgttctgggt agataaggct gatgtccgta gtgtcctgca gtatctcaaa
     1381 gatgaggcgg aaaggcctta caggatgctt tatgatctta ctgcgatcga tgagcgttcc
     1441 cgccagaatc gcaacggcca gccggatggc gcttttacgg tagtgtacca cctgctctcg
     1501 gttgaccgca acgaagatat cagaataaaa accgcccttg acggcaaata tccttcaatc
     1561 gatacgatta ctcatatgtg gcacaacgcc aactggtacg agcgtgaagt ttatgatatg
     1621 ttcggcatta aattcgacgg tcatccgaac ctgcgccgaa tcctgatgcc ctggacatgg
     1681 gagggccatc cccttcgcaa ggagcacccc gcgcgagcca cagagatggg ccagttttcg
     1741 cttcctcagg aaaaacagga ccgtgaacag gaggcgatgc ggttcaagcc cgaggaatgg
     1801 ggtatgaaac gcgagagcga ggacacggaa tttatgttcc tcaatctcgg cccccagcat
     1861 ccgggcaccc acggcgtcct gcgtctgata ctgcagatcg atggcgagat gattgtcgat
     1921 atcgttcccg acatcgggtt tcatc
//
LOCUS       DSEB01000086            1870 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1011780, whole genome shotgun sequence.
ACCESSION   DSEB01000086 DSEB01000000
VERSION     DSEB01000086.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1011780"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1325)
                     /locus_tag="ENO07_01620"
     CDS             complement(<1..1325)
                     /locus_tag="ENO07_01620"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: protein motif:HMM:PF00368.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDS00694.1"
                     /translation="MRPEFIPENCWSGLKTRKVIWEIKDKMKKVNLVHRKSSLVPELK
                     TSRIPGFYRLPVERRLAYLARTFKLSEQQISMLRNGNALRVEHAVNMVENSIGVFGLP
                     LGLGLNFIVDGREHLVPMAVEEASIIAAASKAALLIRKGGGFYTKVDDPVMIGQIQVL
                     DLPDPKKSADAVLANKQRIIDAANQSAIRMRKRGGGVFDIEARILDEKSKVGPMLAVH
                     LLYDVCEAMGANAVNYACESAGPIIEEITGGRVNLRILSNLADRRVARAEFNLPVEYL
                     ATKNMSGLEVARRMVEASEFARIDPYRAATCNKGIFNGVCAAAVALGQDWRAIEAGGH
                     AYAARNGRYSSLTSYDYEDNCLKGRIELPMQVGWVGGAVNSHPGVKLLREISGVRNSR
                     QLAGLLVSVGLGQNFTANLALATDGIQKGHMALHARSVAISVGVPANQV"
     gene            complement(1249..>1870)
                     /locus_tag="ENO07_01625"
     CDS             complement(1249..>1870)
                     /locus_tag="ENO07_01625"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:PRK05437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDS00695.1"
                     /translation="LNPGQELMQAEGHRNFRGNLDMIARLVDRLEGRVLVKETGAGLS
                     PQVLERLSAIGVRYVDVSGSGGTSWTKVESYRAQSKILRSAGKTFSNWGVPTAYSLIN
                     ARRILPEEVCVIGSGGIENGLDSARAIALGADIAGFARAVLMAFMDNGVQGASDFIES
                     NIYELKTAMLLTGSRRTGDLQNAPRVYTGKLLEWLKNSESHLGDKG"
BASE COUNT          426 a          569 c          426 g          449 t
ORIGIN      
        1 tccacctggt tggccggaac gcccacagaa atcgccacac tcctggcatg cagggccatg
       61 tgtcccttct ggatgccgtc ggtggccagc gccaggttgg cggtgaaatt ctgccccagc
      121 ccaaccgaaa ccaaaaggcc ggccaattga cgtgaattcc gcactccgct tatttcacgc
      181 agcagtttta caccgggatg agagtttaca gctcctccaa cccatccgac ctgcatgggc
      241 agttctatcc tgcccttgag acaattatct tcataatcat atgaagtaag gcttgaatac
      301 ctgccattac gtgcggcata ggcatgaccg cctgcctcaa tagctctcca gtcctgtcca
      361 agggctactg ccgcggcgca cacgccgttg aagatacctt tgttgcaggt cgctgctcta
      421 tacgggtcga tacgcgcgaa ctcacttgct tcaaccattc gtcgggcaac ctccaagcca
      481 gacatattct tagtcgccag atattcgaca ggcagattga attctgcccg cgccacccgc
      541 ctgtcagcga ggttcgagag tatgcgaaga tttacacgtc cgccggtgat ctcctcgatg
      601 atcggtcccg ccgattcaca agcataattt accgcgttgg cgcccattgc ctcgcagaca
      661 tcatagagca gatgcaccgc cagcatcgga ccgaccttgc tcttctcatc cagaattcgc
      721 gcctctatat caaagacccc gcccccgcgt tttctcattc tgattgccga ttggttggcg
      781 gcatcgatta ttctctgctt atttgcaagc acggcatctg cagatttctt tggatcgggt
      841 agatcgagta cttgaatctg gccgatcata acaggatcat caactttcgt gtaaaagcct
      901 cctcctttac ggatcaacaa tgcagctttt gaagcggcgg ctataatcga ggcctcctcc
      961 accgccatcg gtaccagatg ttcgcggccg tccacaatga aattcagccc cagacccagg
     1021 ggcaggccga atacaccgat cgaattctcc accatattga ccgcatgttc aacacgcagc
     1081 gcattgccat tcctgagcat ggaaatctgc tgctctgaaa gtttgaatgt acgtgccaga
     1141 taggcaagcc tgcgctccac cggcagtcga tagaatcccg gtatcctcga ggttttgagc
     1201 tcgggaacaa gtgatgattt cctgtgaacc agatttactt tcttcatctt atcctttatc
     1261 tcccagatga ctttccgagt ttttaagcca ctccagcagt tttccggtat aaactcgggg
     1321 cgcattttgc aggtcccctg ttcggcgcga accggtcaac agcatggcgg tcttgagttc
     1381 ataaatattg ctctctataa agtccgaggc tccctgaacc ccattatcca taaaagccat
     1441 cagaacggcc ctggcaaagc ccgcgatatc ggctcccaat gcgatcgcac gggccgaatc
     1501 caatccgttt tctatgccgc ccgatccgat tacacaaact tcctctggaa gaattcttcg
     1561 ggcattaata agactatacg cagtcggcac tccccaattg gaaaatgttt tacccgcact
     1621 gcgtaaaatt tttgactggg ccctgtacga ttccactttg gtccaggatg ttcccccgga
     1681 accggagaca tcgacatagc gcacaccaat cgcggacaat ctttcgagca cctgcggaga
     1741 caggcccgcg ccggtttcct tgaccagcac ccttccctca agacgatcga caagccgggc
     1801 aatcatgtcc agattgcccc tgaaattgcg atgtccttcg gcctgcatca gctcctggcc
     1861 gggattaaga
//
LOCUS       DSEB01000087            3635 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002693, whole genome shotgun sequence.
ACCESSION   DSEB01000087 DSEB01000000
VERSION     DSEB01000087.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002693"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..446
                     /locus_tag="ENO07_01630"
     CDS             <1..446
                     /locus_tag="ENO07_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDS00696.1"
                     /translation="IDMSSIKFVNPAMLEKFKKYQLIRDYMESKEKEIAEYNRKHNID
                     TSELINGRNLTNVGTFRAYVIAYLRSHPMIHNDMTFLVRHLPPGPTGLPIEIYVFSKD
                     QVWANYEAIQADIFDHILAVVPLFELRVFQNPSGNDLRELSKIRY"
     gene            534..1031
                     /locus_tag="ENO07_01635"
     CDS             534..1031
                     /locus_tag="ENO07_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006198271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HDS00697.1"
                     /translation="MMKPVRIEPGPGQESVWDYPRPPRMEDFTKKIEIIFNGATIADT
                     VRSKRILETSHPPVYYIPPEDIVMEFLQQSDKESFCEWKGKAKYYHVVINQARAENAA
                     WYYPDPNPEYADLKDYVAFYASPMDACFVDGEMAIPQPGNFYGGWITKDIVGPFKGEP
                     GSMNW"
     gene            complement(1513..3441)
                     /locus_tag="ENO07_01640"
     CDS             complement(1513..3441)
                     /locus_tag="ENO07_01640"
                     /inference="COORDINATES: protein motif:HMM:PF02412.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HDS00698.1"
                     /translation="MRKAILILALLLITFSTAEAADLFLVQVHGYNEAEILRNSTFTV
                     VLRLANDHLVLADRNDLEDSGLDFEILAEDIEIDNLALDRRRDNQNVEKYELLYEKDA
                     VRLLKVAPGDLGNVELAPDLLPLRPERAVIKYVEPSAVKKSLDFQDLDLDSLINLVVQ
                     DSVETYLYRLEAFFRRVAGSDSAYAARDWIVSKFEQFGYDSIYLDFFMADIFYTGEKP
                     CYNVVAVKEGTVYPDLQIVIGGHYDGVPDSPAADDNGTGTVGVLEIARVLQDIETELT
                     FVFIAFDAEETGLDGSTHYSEMAKNRGDDILFMFNMDMIGNFENTDKAIVYHGDNDLY
                     AQLWIDAALPLVGIQGYLGGWSPSSDHFPFTELGYNGVFLHEYEFSDVYHSSHDSTTY
                     VSFDYATRMIKGTLALVYTISQTNDFDQDGIANAGDNCVMVPNADQVDYDADGVGDAC
                     DNCPEEPNPDQSDEDYDGIGDACDGNIHIAGEAPPVGHVGLEYFYEFEGFGGTAPYSW
                     RKIFGQLPYGLTLHDDTIGTLDGVPTWASTFVFEMEMTDAGNPPLKDTATFSITITDP
                     EYVCGDANNDGDVNLTDAVYIINYIFIAGPPPDPLLSGEVNCDGTLNVSDGVWILNYV
                     FIGGSEPCDTNDDGVPDC"
BASE COUNT          995 a          890 c          841 g          909 t
ORIGIN      
        1 atatagatat gtcctcgatc aaattcgtca accccgccat gctggagaaa ttcaaaaaat
       61 atcaacttat cagggattac atggaatcca aggaaaagga aatagcggaa tacaaccgga
      121 aacacaatat tgacacatca gagctgatta acggcaggaa tctcacaaat gtcggcacct
      181 tcagggctta tgtaattgcc tacctgagga gtcatcccat gatacacaat gatatgacct
      241 tcctggttag gcatcttcct cccggcccga ccggtttgcc gatagaaata tatgtcttca
      301 gcaaggacca ggtctgggcc aattatgaag ccattcaggc cgatatcttc gatcatatac
      361 tggcagtcgt cccgctgttt gaattgcgcg tattccagaa cccgtccgga aacgacttac
      421 gcgagctctc gaaaattcgc tattaattcg ggccgcagac aactggaccg tttcggtaaa
      481 tatagagttt atcagtgcgg ttatacaatt ccagatgtta caaaggaagg aagatcatga
      541 agcctgtgag aatagagccg ggtcccggcc aggaatcggt gtgggactat ccccgcccgc
      601 cgcgcatgga ggattttacg aaaaaaatcg agatcatctt taatggagcc actattgcgg
      661 atacggttcg ctcgaagagg atcctcgaaa ccagccatcc gccggtatat tacattccgc
      721 cagaggatat cgtcatggag ttcctgcagc agtcggataa ggaatcgttt tgtgaatgga
      781 agggcaaggc taaatactac cacgtagtta tcaatcaagc cagagccgag aacgccgcct
      841 ggtattatcc tgatcccaat ccggagtatg cagatctcaa ggactatgtc gcattttatg
      901 cgtcgcccat ggatgcctgt tttgtggatg gtgagatggc aattccccag cccggcaatt
      961 tttacggggg atggattacc aaagacattg tcggaccttt taaaggcgaa ccgggcagca
     1021 tgaactggta atccgggata acagccgttt ttatgtttct acgtataaat acgcctttaa
     1081 aagcatgtaa ggtaatgctt taataaggca tgacaggaat ttaaaagcat cccgggcatt
     1141 caattttcct tgacaaaaaa gcctatcgta tataactttt tgatgcgctc ttgaagtata
     1201 tgatgtgcgt ccccaatcgt tagcatggcg atatcaaact ttaagaggcg ctcaccgcat
     1261 caacggatga tgcggtgggt ttttttgaaa aagtcgacac agtagcgata gatcaggctc
     1321 atccagattt cacccaagat tatccgccca ccgttttaca gtgttgtcgt ttgcatcaga
     1381 gctgccctgc tgctgtcggc gtttgcggac ataagccgga caagaaactg agacaggacg
     1441 actaaaacgg tgggccagat attaaaaagc ccgcccctga tcggcgggct ttttttattc
     1501 atacaagcag gatcagcagt cgggaacacc gtcatcattg gtgtcacagg gctcggaacc
     1561 gccgatgaac acataattca aaatccagac cccatcgctg acattaagcg taccgtcaca
     1621 gttcacctca cctgaaagga gtggatcagg cgggggccca gcaataaata tataattgat
     1681 tatataaaca gcatcagtta aattaacatc cccatcatta tttgcatcgc cgcaaacata
     1741 ttcgggatct gtgatggtta tgctgaaggt agcggtatcc ttgagaggag gattgcccgc
     1801 atcggtcatc tccatctcga aaacgaaggt cgaagcccag gtgggtacac catccagggt
     1861 accaatcgtg tcatcgtgta aagtcaaacc gtagggaagc tgcccgaaga tctttctcca
     1921 gctatatggt gcggttccgc cgaatccctc gaattcatag aagtactcta atcccacatg
     1981 accgaccgga ggggcttcac cggcaatatg aatgttgccg tcgcaggcat ccccgatgcc
     2041 atcatagtct tcatcgctct gatccggatt cggctcttcg ggacaattat cgcaggcgtc
     2101 gccaacacca tcagcgtcat aatcgacctg gtcggcattg ggaaccatca cacagttgtc
     2161 ccccgcattg gcaattccgt cctggtcaaa atcattggtc tgggaaatag tataaaccag
     2221 tgccagggtg cccttgatca tgcgtgtggc ataatcgaag ctgacataag tggtgctgtc
     2281 gtggctggaa tgatatacat ccgaaaactc atattcatgc agaaagacgc cgttgtagcc
     2341 cagctcggta aatggaaaat ggtcggaact gggcgaccat ccgcccagat atccctggat
     2401 tcccaccagg ggtagagccg catcgatcca gagctgtgcg tagaggtcgt tatcaccgtg
     2461 ataaacaatt gccttgtcgg tattttcgaa gtttccaatc atatccatat tgaacatgaa
     2521 gagaatatca tcgcccctgt tttttgccat ctcggaataa tgggtggaac catcgagacc
     2581 ggtctcttcc gcgtcgaagg caataaaaac aaaggttaac tcagtctcta tatcctggag
     2641 tacccgggcg atttccagca ctcccaccgt gccggtgcca ttatcatcgg cggcgggtga
     2701 gtccggcacg ccatcataat gtcctccgat tacgatctgc aggtcgggat agacagtccc
     2761 ttccttcacc gcaacgacat tgtaacacgg tttctccccg gtatagaaaa tgtcggccat
     2821 aaaaaaatcc aggtaaatcg aatcgtagcc gaactgctcg aacttggata cgatccagtc
     2881 acgggcggca taagcggaat cgctgcccgc caccctgcgg aagaatgcct ccaggcgata
     2941 aaggtaggtt tcgaccgaat cctgtacaac cagatttata agtgaatcga ggtcaagatc
     3001 ctggaaatcc agactttttt tgactgcaga tggctccacg tactttatga ccgccctttc
     3061 cgggcgaagc ggcagaagat ctggtgccaa ctcaacattt ccaaggtccc ccggcgcaac
     3121 tttgagcagc ctcacggcat ccttttcata cagaagctca tacttctcga cattctgatt
     3181 atccctgcgg cggtctaatg ccagattatc gatctcgata tcttccgcca gtatttcgaa
     3241 atcaagaccg ctgtcctcca gatcgtttcg atccgccagt accaggtggt catttgcgag
     3301 cctgagtacc accgtgaacg tggaattcct cagaatttca gcttcattat aaccatgaac
     3361 ttgtaccagg aagagatcgg cagcttcagc cgtggagaaa gttattaaca ataaagccag
     3421 tatcaggatt gcttttctca taatgtgtta tcccttgtct gtaattgtgc atcctatata
     3481 taagaagtat taaaaccagt attgtttaat cgcaaggccc aaaaaaaatt ggcttatcct
     3541 gtttttttcc aggaaagatg agttattcaa ttcaggtatg aatttactgc aaagttaaat
     3601 tttgtcaata ataatggtaa tttactcctg aacat
//
LOCUS       DSEB01000088            3093 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003854, whole genome shotgun sequence.
ACCESSION   DSEB01000088 DSEB01000000
VERSION     DSEB01000088.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003854"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..929)
                     /locus_tag="ENO07_01645"
     CDS             complement(<1..929)
                     /locus_tag="ENO07_01645"
                     /inference="COORDINATES: protein motif:HMM:PF07728.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDS00699.1"
                     /translation="MDELRSKILLVDDDRSVLEMLDELFSDDYTTILASSGPEAIQKA
                     RENLDVACALMDIKMPDMDGIEANREIKRFLPDLPVIFHTGYPGDYLEDEIEATEKPF
                     DYVLKGDSIPRLTRSVKHAVESHQLKHDNRRLTRYAEHNFGIVGKSKKMGEIYELIRK
                     LSNCVASAVVLGETGTGKELVSKAIHFNSERRDKNFGILNCNHKNSDLVESDLFGHKK
                     GAFTDAKDDRIGLFESANGGTVFLDDIGDLALETQNKLLRVLDTGEFYRMGDSKLRKC
                     DVRVICATHRDLDKMVEKGDFRRDLYYRLKTII"
     gene            complement(978..>3093)
                     /locus_tag="ENO07_01650"
     CDS             complement(978..>3093)
                     /locus_tag="ENO07_01650"
                     /inference="COORDINATES: protein
                     motif:HMM:PF02518.24,HMM:PF13426.5,HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HDS00700.1"
                     /translation="VDAEGVFHFVNQKGAQAHGRDRTEIIGNKMHDLFPKEVADWQLE
                     RVKEVIRTGKEFSEESRILINGDWQWYSTNLQPYRNTEGECVAALVIAQNITEHKEAQ
                     DEVHASEGRLQAIVDSTDDLIFVIDNEGKFTYSKVRERSDNLYADPEEFLGKHIAELV
                     PEEISSEFLTHLEIIKKTGETRQMDYCLEISGETRWYSAKLTPLYYEENKLEGVTIVV
                     REITPQVLSNRALKNERDFSHSILRTANSLIVCLDSKARIKIFNNECENVTGYKREEV
                     LGKSWPDIFLPARYRHEGLTDFESWVKNNPSGKYEGPIVTKTGDVRTILWSNSAIFDP
                     ASGEVTAIAIGQDITLRKKIEESHRKSEKKLRTQFKNFPIPSYTWRKKGDDFVLAEFN
                     DAALRITDGKISKFLGKKLSDMYGDRPALIANIHKCYEEESTFLDESDYVYISTGKKR
                     YLLVTYSYVPHNQVMIYTQDVTPLKQAREDKIRTAKDIAGGFAHEIRNSLFPAKGALS
                     LLAKTKDGNGQAEERRSKYLKITEQSVEKAIDVTNQISLYTRMDSEYFPEKVNLCSVV
                     NKVISSNQLTLEKMRAKVSFEGVKDVEVQSNRKQLYQVVNNLLLNSIDALTKSPEPAI
                     LLHWKQIDSSIVLDFADNGVGIPEENLQRVFDAFYSTKPDNGTGIGLAMAKRIIEMYD
                     GEISVSSKLGKGTTFRLKFKPA"
BASE COUNT          723 a          766 c          660 g          944 t
ORIGIN      
        1 attatgatgg tcttgaggcg gtaatagaga tcgcggcgga agtctccctt ctcaaccatc
       61 ttatccaggt cgcggtgagt ggcgcagata acacggacat cacattttcg cagctttgaa
      121 tcacccatac gatagaattc tcccgtatcc agcacccgca gaagcttgtt ctgggtttcc
      181 agcgccagat ccccgatatc gtcaaggaaa acagtcccgc cgttggccga ctcaaacagc
      241 ccgattcgat catcctttgc gtcggtgaat gctcccttct tatgcccgaa cagatcggat
      301 tcaaccaaat ctgaattttt atgattgcaa ttcaggatac caaaattctt gtcccgtctt
      361 tcactgttga agtggatcgc cttcgatacc agttccttgc cggtgccggt ttcgcccagc
      421 acgacagcgg aggctacgca attggagagc ttgcggatca attcgtaaat ctcacccatt
      481 tttttggatt tgccgacaat tccgaagttg tgctcggcat atcgggtcag tctgcgatta
      541 tcatgtttta attgatgact ttccacggca tgcttgactg aacgggtaag gcgcggaata
      601 gagtcaccct tgagaacata atcgaagggc ttttcggtag cctcgatctc gtcctccaga
      661 taatctccgg ggtagccggt gtgaaagata acaggcaaat cgggaaggaa cctcttgatc
      721 tcacgattgg cttcgatacc gtccatatcc ggcatcttga tatccatcaa ggcgcaggca
      781 acatccaaat tttctctcgc cttctgtatg gcttcaggtc ccgaagaagc caggatagtt
      841 gtatagtcat cactgaaaag ctcatccagc atttccagga ctgatctatc atcgtcgacc
      901 agaagaatct tactgcgcaa ctcatccata aaaaataact accttagata ctcacattaa
      961 aatttcaagt aagaatatta tgctggcttg aatttcaatc taaaagttgt gcctttacca
     1021 agtttgctcg atacagaaat ctcaccatca tacatttcaa ttattctctt agccatcgcc
     1081 aggccgatac cggtaccatt atccggtttg gtggagtaaa aagcatcgaa aacacgctgt
     1141 aaattttcct ctggtatacc cacaccgttg tctgcgaaat caagaacaat actgctgtct
     1201 atctgcttcc agtgtaataa tatagccggc tccggcgatt ttgtcaaggc atcaatgcta
     1261 ttcagcagca aattattgac aacttgatag agctgctttc tattcgattg aacctcaaca
     1321 tcttttactc cctcgaaact gacttttgct ctcattttct ccagagtaag ttgattggag
     1381 ctgataactt tgttaaccac gctgcacagg ttcactttct ccgggaaata ttcactatcc
     1441 atcctggtgt aaagcgaaat ctgattggta acatcgattg ctttttcgac ggactgttcg
     1501 gttattttca ggtattttga acggcgttcc tcggcctgac cgtttccatc ttttgttttg
     1561 gccagcaggc ttaaagctcc tttggcgggg aagagtgaat ttcgaatttc gtgggcaaag
     1621 cctccggcaa tatctttggc ggtcctgatc ttgtcttccc tggcctgctt gagcggcgtc
     1681 acatcctgcg tatagatcat cacctggtta tgtggaacat aagaatatgt aaccagcaga
     1741 taacgtttct tgcccgtgct tatataaacg taatccgact catcgagaaa ggtggattcc
     1801 tcctcataac atttgtgtat gtttgctatg agagcgggcc gatccccgta catgtcagac
     1861 agcttcttgc cgagaaattt gctgatcttt ccgtcggtaa tcctgagggc cgcatcattg
     1921 aattccgcga gtacgaagtc atcacctttt tttcgccagg tgtagctggg gatgggaaaa
     1981 ttcttgaatt gggttctgag cttcttttcg ctttttctat gcgattcctc gatcttcttt
     2041 cgcaaagtta tatcctgtcc aatcgcgatt gcggttacct cgccgctcgc cggatcgaaa
     2101 attgcactgt ttgaccagag gattgtgcgt acatccccgg ttttagtcac aatcgggccc
     2161 tcatatttgc cactgggatt attcttcacc cagctttcga aatcggtcag tccttcatgc
     2221 cgatatcgcg ccggcaggaa tatatccggc caggatttgc ccagcacttc ttcacgctta
     2281 taaccggtta cgttctcgca ttcgttgttg aatattttta tcctggcctt actatccagg
     2341 catacgataa ggctgttggc cgtgcgcagg attgaatgtg aaaaatctct ttcgtttttg
     2401 agcgcgcggt tggacaggac ctgaggtgtg atttccctga ctacgatggt tacgccttcc
     2461 aatttatttt cttcataata taggggcgtg agttttgccg aataccagcg agtttctccg
     2521 cttatttcaa gacaatagtc catttgccgg gtttcacctg ttttcttaat gatttcaaga
     2581 tgtgtcagga actcggaaga gatttcctcc ggcaccagtt cggcaatgtg ttttcccaga
     2641 aactcctctg gatcggcata taagttatct gatctctccc tcactttgga ataggtaaat
     2701 tttccttcat tatcgatcac gaaaataagg tcatcggttg aatcgacgat tgcctggaga
     2761 cgtccctcgc tggcatgtac ctcgtcctgc gcctctttgt gctcggttat attttgggct
     2821 atgacaagag ctgcgacaca ttcaccctca gtatttctat agggctggag atttgtggag
     2881 taccattgcc agtcgccatt gatcaaaatc ctcgattcct cgctgaattc cttgccggtt
     2941 cttattacct cttttactct ttccaattgc cagtctgcaa cttctttcgg aaatagatca
     3001 tgcattttat ttccgattat ctcagtgcgg tctcttccgt gtgcctgtgc tcctttttga
     3061 ttcacaaaat gaaatacacc ctcggcatca acg
//
LOCUS       DSEB01000089            1950 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1010657, whole genome shotgun sequence.
ACCESSION   DSEB01000089 DSEB01000000
VERSION     DSEB01000089.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1010657"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            46..1032
                     /locus_tag="ENO07_01655"
     CDS             46..1032
                     /locus_tag="ENO07_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDS00701.1"
                     /translation="MNQDIKEIQAEVEEKSKFLQNLTSEIRKIIVGQNYLIDRLLVGL
                     LADGHILIEGVPGLAKTLSIKTLSDCIQTRFQRLQFTPDLLPADLIGTMVFSPQEGKF
                     YPKKGPIFANLILADEINRAPAKVQSALLEAMQERQVTIGDTTYPLDEPFMVLATQNP
                     IEQEGTYPLPEAQVDRFMLKIKITYPGREEELQIMDRMTVGQIFELTKVTSPEEILNA
                     RKVISGIYVDEKVKEYIVSIVMATRDPEAFGLDLSQYIAYGASPRASIYLNLASKAHA
                     FLRGRGYITPEDVKQIGMDVLRHRVILTYEAEAEELTSEDIIQKIFDEVEVP"
     gene            1073..1918
                     /locus_tag="ENO07_01660"
     CDS             1073..1918
                     /locus_tag="ENO07_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HDS00702.1"
                     /translation="MIEIRTRRLVNDLFSGEYHSIFKGRGMEFDEVREYQIGDDVRQI
                     DWNVTARTGTPFIKKFTEERELSVVFLIDASGSQHFGSGQRLKSELAAELTALLSLAA
                     IKNNDKVGMIIFTDKIEKFVPPNKGRKHVLRLIREVLYFKPEEKQTNISNALEYFLNV
                     IKRRSVVFLISDFYASDFERELLLAGRKHDLVAIRLTDPREMEIPPCGLVYMEDAETG
                     KPLWLDTNDAEFRRQFSENRREELRQIERMFKQNNVDFIDLDTSGSYIEPLIKFFKMR
                     EKRIH"
BASE COUNT          512 a          447 c          540 g          451 t
ORIGIN      
        1 atacatagat aattcgaaaa cgaaaagcga atcgggagat agagtatgaa tcaggatatt
       61 aaagagattc aggcagaggt tgaagagaag tctaaatttt tgcagaattt aacgagcgaa
      121 atcaggaaga taatagtcgg acagaattat ctgatcgatc gtcttttagt cgggcttctg
      181 gcggacggtc atatcctgat cgagggcgtt ccgggattgg ccaagaccct ctcgatcaag
      241 acactatcag attgtatcca gacccgcttc caaaggctcc agttcacccc tgatcttctg
      301 ccggcggatt tgatcgggac catggtgttc tcaccccagg agggcaaatt ttatcccaag
      361 aagggtccga ttttcgccaa cctgattctg gccgatgaga tcaaccgcgc accggccaaa
      421 gtccaatcgg cgctgctgga ggcgatgcag gaacgtcagg ttactattgg agataccact
      481 tatccattgg atgagccctt catggttctg gccacccaga atcctatcga acaggagggc
      541 acatatccat tacctgaagc tcaggttgac agatttatgc ttaaaatcaa gatcacctac
      601 ccgggacgtg aggaagagct ccagattatg gaccgcatga cagttggcca gattttcgag
      661 cttacaaagg tgacctctcc ggaggagatc ctgaatgccc gtaaagtaat cagcggtatt
      721 tatgtggatg agaaagttaa ggaatatatc gtcagtatcg taatggccac ccgtgatcca
      781 gaggctttcg gcctcgatct ctcccagtat atcgcctatg gggcctcgcc gcgagccagt
      841 atctatctca atctggcatc caaggcgcat gcatttctgc gggggcgggg atatataact
      901 cccgaggatg tcaagcagat cggtatggat gtgctgcggc accgggtgat cctgacttac
      961 gaggctgagg ccgaggaact gacctcggaa gatatcatcc agaaaatatt tgacgaagtc
     1021 gaggtgccgt agcgtgacag tcgtcccatc cgaaataatc aggaaggtcc gcatgatcga
     1081 aatccgtacc cgcaggctgg tgaacgatct tttttccggc gagtaccatt ctatttttaa
     1141 ggggcgcggg atggaattcg acgaggtgcg cgagtaccag atcggggatg acgtacgcca
     1201 gatagactgg aacgtgactg cccgcacggg cacaccgttt atcaagaaat tcaccgagga
     1261 gcgagagctc tcggtggtat ttttgataga tgcttccggg tcgcaacatt tcggaagcgg
     1321 gcagcgctta aagagcgagc tggcagcgga gttgaccgcg cttctgtcgc tggcggcgat
     1381 taaaaacaac gacaaggtgg ggatgattat atttacggac aagatcgaaa aattcgtacc
     1441 gcccaataag ggccgcaagc atgtcctgag gcttattcgt gaggtgctct atttcaaacc
     1501 ggaggagaag cagaccaata tttccaacgc gctggaatat ttcctgaatg ttatcaagcg
     1561 gcgctcggtt gtgttcctga tttcagattt ttatgcttcc gattttgagc gcgaactgct
     1621 gctggccggg cgcaagcatg atctggtggc gatcaggctg accgatccgc gcgagatgga
     1681 gattccgccc tgcgggctgg tgtatatgga ggatgccgag accggcaaac cgctctggct
     1741 ggatactaat gacgccgagt tcaggcggca gttttccgaa aatcgcaggg aggaattgag
     1801 gcagatcgag cggatgttca aacagaataa tgtcgatttt atcgatcttg acacctccgg
     1861 atcatatata gagccgctga ttaaattctt taaaatgcgg gaaaagagga ttcactgaag
     1921 catgaatgaa acggccgcag atataaggca
//
LOCUS       DSEB01000090            1978 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1010343, whole genome shotgun sequence.
ACCESSION   DSEB01000090 DSEB01000000
VERSION     DSEB01000090.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1010343"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="ENO07_01665"
     CDS             complement(<1..1136)
                     /locus_tag="ENO07_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00703.1"
                     /translation="MKNSKRTHKTVRFFMLTLFLSLPLILIGGDYSITDDPCARYIGE
                     SWRSGNLCPGSEITLLGNDTYRAEFESLIDGNHNTGMACEFANSNGIQIRFFGRVPWV
                     NVIRIYHKGDCSFDLSVKDIYKKETQADFMMHETIGKDGMKISVYCCNDMRISAIVLT
                     RDNSNANSKGDGTLEIFEVEAFFESADSDDGDEEVGVEYVQYYKHKPCGGSNIKGTKS
                     DALDFRDKLYNKLGWTRKFTWSNYWAFEMDFNIHDDTRADDVDFAYFCGHGNNGKFIF
                     SHMNMDCSMYYTECLNRWGDNDMEWIAVASCRTLRQTNSNQYWSGWYRCFAGLHVMLG
                     WHTNMLDVNIGTRFGNQLAKKHRTIWTSWKNAARKSHYVNLWTHT"
BASE COUNT          519 a          471 c          395 g          593 t
ORIGIN      
        1 gtatgtgtcc agaggttgac atagtggctt ttccgcgcgg cgtttttcca ggaggtccat
       61 attgtgcgat gcttcttcgc aagctggttt ccgaagcgtg ttccaatgtt tacatccagc
      121 atatttgtgt gccaaccaag catcacatgt aatccagcaa agcacctgta ccatcccgac
      181 cagtattgat tcgaattagt ttgacgcaaa gttctgcatg aggcaaccgc gatccattcc
      241 atatcattat ctccccatct attgaggcat tcggtgtagt acatactgca gtccatattc
      301 atgtgagaaa atataaactt accattgttc ccatgcccgc agaaataggc aaaatctaca
      361 tcgtcggcac gggtatcatc atgtatgtta aaatccattt caaatgccca ataatttgac
      421 catgtaaatt ttctcgtcca gcccagctta ttgtatagtt tatctctaaa atccagcgca
      481 tcgctttttg tacctttgat attagaacct ccgcatggtt tgtgcttata atattgaacg
      541 tattcgacgc caacctcctc atcaccatcg tctgagtccg cgctttcgaa aaaggcttct
      601 acttcaaata tctccagagt tccatcgcct ttagaattcg cattcgaatt atcccgtgtt
      661 aaaacaattg cggatatgcg catatcattg caacaataaa cggatatctt cataccatct
      721 ttaccaatag tctcgtgcat cataaaatca gcctgagtct ctttcttgta tatatcctta
      781 acgctcaagt caaagctgca atcgccctta tgatatatcc gtatgacatt aacccagggt
      841 actcggccaa aaaacctgat ctggattcca ttagaattgg caaattcaca ggccataccg
      901 gtgttatgat tcccgtctat taaggattca aactcggccc tgtatgtgtc atttccgagt
      961 aatgtgatct ctgaaccggg acaaagattt ccggatctcc aagactctcc gatatacctc
     1021 gcgcagggat catcggtaat actgtaatca cctccgatca atataagcgg aagactcaga
     1081 aataaagtca gcatgaaaaa tctaactgtt ttgtgcgttc gtttggaatt tttcatttta
     1141 cctccttgga cgtaatgggg ctaaacggga gattattcca tggtgtgaaa acctgaggtc
     1201 atcctcggtg tgaaatatat aaggacaatg taaaatgacc caggggagag cctatttagc
     1261 aaccatgatt atttaattaa ctgcgttggg gcaaactcag tgcaggcaaa tcttcagaca
     1321 gcaatcggac aaattgccaa caactatcta ctgtaaaata aacccgagat gtcttttttg
     1381 tcaaccctaa tatcgacttt tatcttaagt ccaagcggcc caacgtgaat gtcaaacccg
     1441 cccattgacg agcgggtttg tgaactaata taattgtcgc ttactgcttt gttgcattta
     1501 tgacgcgttt tttctcatga tcggagggtt taattattcc catctctgga gacagcccgt
     1561 attcttcagg atgacggtaa tagccctgtt cttcggatgg tttgtctgtc tcgacctgtg
     1621 ctctatggga atttgcatag gcatctgccc gaatacctgt aacctgccat gagactctca
     1681 tgcccggttc tcctccggct atgcggaaac tgtttccact gatttcttcc gcaatgtata
     1741 gatcaggtcc cggcatcccg atcgctgtaa gctggtagcg gaaatcccga ttcaaggctt
     1801 cgaagtattc aggcagattc acgttcgcct cgcccttcgc atcaagtgta atgttaccgt
     1861 tatatatatt catcatgtcc ggcgagctga ccgtactgtg gctgagatat ttattttgcg
     1921 gatcgagtgg atgatcgata ttgtatgcgg acgccgactt atcaatcgtg cccgtaac
//
LOCUS       DSEB01000091            1908 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1011224, whole genome shotgun sequence.
ACCESSION   DSEB01000091 DSEB01000000
VERSION     DSEB01000091.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1011224"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            539..1462
                     /locus_tag="ENO07_01670"
     CDS             539..1462
                     /locus_tag="ENO07_01670"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HDS00704.1"
                     /translation="MRFRFGLFLILLLLPGVLYGQAKVATAGAQFLEIGVSARAVAMG
                     ESFISIADDASAVYYNPAGMTQIYEKEFMFSHIDYAADMNYEFGAFVLPMWSLGGVLG
                     FGVYMFDAGEIEETTYGHATGTGRTFGAKEYALSASYARSLTDHFSVGVTVKLIDQLY
                     ESERSTGWAADVGTIYDTGFRGFRISMMISNFGPDMTFISESAPLPINFKFGGAFNVI
                     ESERHRATFSVEGSHPSDNLEKYSTGVEYSFNEIFSLRMGQRFQIDTGGFSAGAGVKL
                     PISDYNVRLDYAYFDFGVLESVHRFSIGFSL"
     gene            1500..>1908
                     /locus_tag="ENO07_01675"
     CDS             1500..>1908
                     /locus_tag="ENO07_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00705.1"
                     /translation="MHNKHAKILLFVAAALIATTLIFAGCSDTEPGGPLPPNAAPKCY
                     LANIPTEGEEYSYNPTLYWYATDEDGFISQYRYVVKKAEDINDDPLAFAESIMNNGDF
                     DDWTVINTDALNPGQSATSNTVQLYAVADPEVYT"
BASE COUNT          463 a          406 c          530 g          509 t
ORIGIN      
        1 aatatacata cgaaatcgag ggtctcaggc catcacaatt caaatatttt gctgtaactt
       61 ccttcgatta cggtaatccg gaaaatgacc tgctgcccct ggaatcatca ccgctatcca
      121 attatattaa agtatatccg atctactcag ctgaaattgc ggaggaaacc ggggcggaaa
      181 tttcggtata tcccaacccg taccgtatcg atgccggtta ctccgaggat ggttatgaaa
      241 atccccgcgg gcgcggtgtg gaatatgagc gccggattca cttcatcaat cttccggagg
      301 aatgcacgat caagatctgg accaccgacg gcgacctggt acgcgaaatc aagcatggtc
      361 cgggagagcc tttctcggaa accaattcca aggcttactg ggatttgatt acccgtaaca
      421 cacaggcgat tgtaagcgga atctacattt atagtataga atccgagagg ggtactgagc
      481 ttggaaagat tgttgtgatt aaataatgag ggaggcttaa gcgcttaagg tgacaattat
      541 gagatttaga tttggtttgt ttttgatcct gctgcttttg cctggggtat tgtatggtca
      601 ggccaaggtg gcgacagcgg gggcgcagtt tttggagata ggtgtatcag cccgggcggt
      661 agctatgggt gagtcgttta tatcgattgc tgatgatgcc tcggcggtat attacaatcc
      721 tgccggcatg acccagatat atgagaagga gttcatgttt tcgcacatag actatgcggc
      781 ggacatgaac tatgagtttg gggcctttgt gcttccgatg tggagcctgg gaggagttct
      841 gggctttggc gtgtacatgt ttgatgctgg agagatagag gagacgacgt atggtcatgc
      901 gaccggtacg gggcggacat ttggcgcgaa ggaatatgct ttgagcgcca gttatgcccg
      961 cagtttgacg gatcattttt cagtaggagt gacggtcaag ctgatcgacc agctgtatga
     1021 gtcggagcgt tcgacgggct gggctgctga tgtaggcacg atctacgata ctggttttcg
     1081 tggttttcgg atctcgatga tgatatctaa ttttggtccg gacatgacat ttatctcgga
     1141 gtcggcgccg ttgccgatca atttcaagtt tggtggagcg ttcaatgtga tagagagcga
     1201 gagacaccgg gcgacctttt cggtggaggg ttctcatcca tcggacaatc tggagaagta
     1261 ttcgacggga gtggagtaca gtttcaacga gatcttttca cttcggatgg gccagcgttt
     1321 ccagatagac acgggcggct tttcggccgg agcgggagtt aagcttccga tcagcgatta
     1381 caatgttcgt ctggactacg cctatttcga ctttggcgtg ctggaatccg ttcacaggtt
     1441 ctctatcggg ttcagcctgt aaccaggaaa aaatgttctt gttataaatt ggaggaatga
     1501 tgcacaataa gcatgcaaaa atattactat tcgttgcggc tgcactgatt gctactaccc
     1561 tgattttcgc cggttgcagt gatacagaac cgggagggcc tcttccgccc aatgcggccc
     1621 caaaatgtta tcttgctaat attcccacgg aaggtgagga gtattcttat aatcctaccc
     1681 tgtactggta tgcaaccgac gaagatggct tcatcagcca atatcggtat gtggtgaaaa
     1741 aagctgagga tatcaatgac gatcctctgg ctttcgccga gagcattatg aataacggcg
     1801 actttgatga ctggacagtg atcaataccg atgctttgaa cccgggacag tctgcgacct
     1861 cgaataccgt tcagctttac gcagtggcgg accccgaagt ttacacac
//
LOCUS       DSEB01000092            2296 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1007454, whole genome shotgun sequence.
ACCESSION   DSEB01000092 DSEB01000000
VERSION     DSEB01000092.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1007454"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            18..1730
                     /locus_tag="ENO07_01680"
     CDS             18..1730
                     /locus_tag="ENO07_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00706.1"
                     /translation="MKNYMKISLLLLSLMLMLGCSGYKPVLQPEGPYWVDDDKQDIPE
                     PSSKDPNLAWMSIKRSSFDQIEDGLDLERNFRILSDEREEAANINSFDEVPNCTWFTN
                     RHGFARMTPDEIAEGVDLTPGPDTSGPWLVFRPKVQGTTPGFWIEDSRGDQYVIKFDP
                     KGYPEMATAAAAMASRYFHACGYNVPQETIVYYRPEILQVKEGVKFEDKAGVKREFTQ
                     QDLEDILKRVHHEPDGRIRSLASLSLGIHGKIKGPFSYSGRRSDDPNDWFDHQDRREL
                     RGLYVIGSFINHYDLKDQNSLDIYVEENGNKFLKHFLIDFGSTFGSDGQGPKPPIKGY
                     ANMIDLRDAFVSLITVGLKTWDWRGGGEVIYPSIGYFEADIFQPDKFDPIYPNPAFED
                     KTDRDCYWGAKIVMAFRDSDLRALIETGQYSNPQAEEYLFETLKKRRDKIGRFWFSKV
                     NPLDYFETEYAGSDLRIRFDDLGIKYELWPQDAVYDYEIRYGKELIRKDRVKTGEIIL
                     TQDDLNRMTSAFKATDKKEDYIYRMDIRTTRGNGDPSKPTRLHFWYHHDENKFSLVGV
                     EHLD"
     gene            1857..>2296
                     /locus_tag="ENO07_01685"
     CDS             1857..>2296
                     /locus_tag="ENO07_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00707.1"
                     /translation="MNIQNVELQNKLENIFPESEGYLVSGPLCDDTFEDSAYFYCGAV
                     RVICPNLKLYKQGIDIAAGMDSLTAITDYPDPSGSYYAFEKYYTYHNGNLVDTFKFET
                     ANSVRYELALNKELIFSPLRSDVIRMPDSERKEMHDYFLQYDSG"
BASE COUNT          641 a          526 c          552 g          577 t
ORIGIN      
        1 ctctaaggat gcacattatg aaaaattata tgaaaatatc tttattgctg ctgtcactga
       61 tgcttatgct gggttgcagc ggctacaaac cagtcttgca accggagggt ccctactggg
      121 ttgacgacga taaacaggat atcccggaac catcatccaa ggacccaaac ttagcctgga
      181 tgtccatcaa gcgttccagc ttcgaccaga ttgaagacgg cctcgatctg gaacggaatt
      241 tcaggattct atccgatgag cgtgaagaag cggccaatat caacagtttc gacgaggttc
      301 ccaactgcac ctggttcacc aaccgtcatg gtttcgcacg catgactccg gacgagatcg
      361 ccgaaggagt cgatctcacg ccgggaccgg acacaagcgg accatggctt gtatttcgtc
      421 ctaaggtgca gggtaccact cccggtttct ggatcgagga ttcacgggga gatcaatatg
      481 tcatcaaatt cgacccgaag gggtatcctg agatggctac ggcggcggcc gccatggctt
      541 cccgatattt ccatgcttgc ggttataatg tgccccagga aacaattgta tattatcgtc
      601 ccgaaatcct tcaggtcaaa gaaggggtga agtttgaaga caaagctggt gtgaaacgtg
      661 aattcactca gcaggactta gaggatattc ttaaacgagt acatcacgag ccggacggga
      721 gaattcggtc actggcctcg ctttctctgg gaattcatgg taaaatcaag ggccctttca
      781 gctatagcgg aagacggagc gatgatccca atgactggtt cgatcaccag gacaggcggg
      841 aattgcgcgg gctgtatgtc atcggatcgt tcataaatca ttatgacctg aaggaccaga
      901 attccctcga tatctatgtg gaggaaaacg gaaataaatt tctaaagcat ttcctgatcg
      961 atttcggttc caccttcggc tctgacggcc aggggccaaa gccgcctatt aagggctatg
     1021 ccaatatgat cgatcttcgt gacgccttcg tatccctgat aactgtcggc ctcaagacat
     1081 gggactggcg gggaggtggc gaggtaatct acccatccat cggctatttc gaagcggata
     1141 tctttcagcc cgataagttc gatccgatct accccaatcc cgcttttgag gacaaaaccg
     1201 accgcgactg ctactgggga gccaaaatcg ttatggcatt ccgcgacagt gatttacgcg
     1261 ccctgatcga aaccgggcag tattccaatc cccaagccga ggaatatctg ttcgaaaccc
     1321 tgaaaaagcg ccgcgacaag attggacgat tctggttttc aaaggtcaat cccctggatt
     1381 attttgaaac ggaatatgct ggcagtgatc tcagaatcag gttcgatgac ctgggaatca
     1441 aatacgagct ctggccccaa gatgcggtct atgactatga aatcagatac ggcaaggaac
     1501 ttatccgcaa ggatcgggtt aaaactggcg aaataattct gacacaggat gatttaaatc
     1561 gcatgacctc ggcatttaaa gccacggata aaaaagagga ttatatttac aggatggata
     1621 tcagaaccac cagagggaat ggtgatccga gcaagccaac ccgattgcat ttctggtatc
     1681 atcatgatga aaataaattc tcattggtgg gagtcgaaca tctggattga tttgtgatca
     1741 tgaaggtaag tatttttata cccatatcaa ttttatgcct gtttcaggca ttcacatctc
     1801 tccatgcaga atccaatgat ccacatgcaa tcagtggaag tgattttgat catttaatga
     1861 atatccagaa tgttgaatta cagaataaac tcgagaatat atttcccgag agtgaaggtt
     1921 atctggtaag cggacctctg tgcgacgata cgttcgagga ctctgcgtat ttttattgcg
     1981 gtgcggtcag ggttatttgc ccaaacctta aactatataa gcaaggtata gacatagccg
     2041 ccggcatgga cagtctcact gcaattacag attatccgga tccaagcgga tcatactatg
     2101 cctttgaaaa atattatact tatcataatg gtaacctggt cgatacgttc aagtttgaaa
     2161 ccgcaaattc cgttcggtac gagcttgcct taaataagga gctgatattc agcccgcttc
     2221 gaagtgatgt catccgcatg cccgattcag aacgtaagga aatgcatgat tatttccttc
     2281 aatatgactc cgggaa
//
LOCUS       DSEB01000093            2310 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1007351, whole genome shotgun sequence.
ACCESSION   DSEB01000093 DSEB01000000
VERSION     DSEB01000093.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1007351"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..958)
                     /locus_tag="ENO07_01690"
     CDS             complement(<1..958)
                     /locus_tag="ENO07_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00708.1"
                     /translation="MKLNHLFLFGLLIGLMLSANLAAEIPGILNYQGRLTGTDGSPIA
                     DGNYQMQFRIYGSLSGTDVLWSSGAVTVSVSKGLFNIHLGESPMSEIPSSVFAGGTIR
                     YLGITIASDPELSPRAMICSGAYTYRAQHADTADYAHAAPGGDAAWTDDGTRVHLSNS
                     NDSVGVGTDAPQSKLHVSTPATEAKLGQSTYGVYGQHFLTSSYGYLGGSSVGGYGYHG
                     SNYGLWGGTLNGKGVYGSTSDNSGIAVYGQNLNGNYGYLGGNNYAIYGNAASGHAGYF
                     AGNVFIGNNLGLGNTSPLNRLDVEGGAAIGTSYSGTATAPENG"
     gene            complement(1380..2030)
                     /locus_tag="ENO07_01695"
     CDS             complement(1380..2030)
                     /locus_tag="ENO07_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00709.1"
                     /translation="MSSYTWEEGLIINVNSILLAYEPDKYRRIRWFDDNANSISMEYT
                     LDSNGLAMLAIVDGIPVIDDTVEIETSEWNGEDGLWHELSIELIGDQIYGLRDDKVLF
                     EAADSRILNYTHTGHLEISGLSAQLCFDDISFVSLVQPPVLCGDANNDNSVNVSDAVN
                     IINFVFAGGNPPNPYEAGDTNCDGTANVSDAVWIINYVFVGGSAPCDTDGNGDPEC"
     gene            complement(2055..2255)
                     /locus_tag="ENO07_01700"
     CDS             complement(2055..2255)
                     /locus_tag="ENO07_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00710.1"
                     /translation="MLRRFSALAFGILLIASLAVPSQLYGFERDTIFYDDFSTGSLEN
                     WQLILGAWVTDDAKMCQLECSD"
BASE COUNT          596 a          590 c          558 g          566 t
ORIGIN      
        1 agccgttctc gggggcggtt gctgttccgg aataggaagt gccgattgcc gcgccgcctt
       61 cgacgtcaag ccggttcaat ggactggtgt ttcctaatcc gaggttgttg ccgatgaaca
      121 cgtttcccgc aaaatagccg gcatggccgc tggcggcatt tccataaata gcgtagttat
      181 ttccgcccaa atagccgtag ttgccattta aattctgacc atagactgca attccggaat
      241 tatcgcttgt actgccatag actcctttac catttaaggt ccctccccag aggccatagt
      301 tgcttccatg gtatccatat ccgccgacgc tactgcctcc cagatagccg taactgcttg
      361 taagaaaatg ctgtccgtaa acgccatagg tgctctgacc taatttggcc tcggtggcag
      421 gcgtggagac atgcagtttg gactggggag catccgtgcc gactcccaca ctgtcgttgc
      481 tgtttgacag atgaacccgg gtgccatcat ccgtccaggc agcgtccccg cccggcgcag
      541 cgtgggcata atcggcggtg tcggcatgct gggcgcggta tgtgtaggcg ccggagcaga
      601 tcatcgcacg cggcgacagt tctggatcgg aagcgatggt aatgccaagg tacctgatcg
      661 tgccgccggc aaagaccgag gacggtattt ctgacatggg agattctccc aggtggatat
      721 tgaaaagtcc tttgctcaca gaaaccgtca ctgcaccgct tgaccagaga acatcggtgc
      781 cggaaagtga accgtagatt ctgaattgca tctgataatt tccgtccgcg atcgggcttc
      841 cgtctgtacc ggtaaggcgc ccctgataat tcagaatccc cggtatttcg gccgccagat
      901 tagcagacag catcaggcct attagaaggc cgaacagaaa aaggtgattg agtttcattt
      961 tgtcctccca aagagaaatg tatttgttta gtttctaatc agattcggtg ggcccaagga
     1021 aataccctgt caagattgcc gcaggagtct gtgcatgttc ttgatgttgc aggaaattcg
     1081 acaaggtttg agcccgggga aaaacaaccg gatatatccc ccataaaaga gttgcgcact
     1141 tgccatgcag tccaaccgca ggcgaaagac atagctttga acctgatata acctgaatca
     1201 acagcccctt agataaccac ccctttgaag atgtcttatg cggatgtcaa aaatatacct
     1261 tgttttaatg aattgtcaat tataaacttg tccgtcaagt ttataactgc aagcggctaa
     1321 taaaaaaccg cccctgatat tttcagggac ggcataaggg aggtattaaa atgccatgac
     1381 taacactccg gatcaccgtt tccgtcggta tcgcatggag cactgcctcc gacgaaaaca
     1441 taattgatga tccagacggc atcggagaca ttggcggtac catcacaatt ggtatcgccg
     1501 gcctcatatg ggttcggcgg attaccgccg gcaaaaacaa aatttatgat gtttactgca
     1561 tcgctgacat tgacggagtt atcgttattg gcatcaccgc ataaaaccgg aggttgaacc
     1621 agtgaaacaa atgagatatc atcaaagcaa agctgggcgc tgagtccgga aatctccagg
     1681 tgcccggtat gggtgtaatt caaaattcgg ctgtctgcag cctcgaacag gactttatca
     1741 tcacgcagac cgtaaatctg gtcgccgatc agctctatcg agagctcatg ccacaggcca
     1801 tcttcaccgt tccactcaga tgtttcaatt tccacagtgt catcgataac aggaattccg
     1861 tccacgatag ccagcatggc taggccgtta ctgtccaggg tgtattccat agaaatcgaa
     1921 ttggcgttat catcaaacca tctgatacgc cggtatttat ccggctcata tgccagaaga
     1981 atcgaattca cattgattat cagcccctct tcccatgtat acgaactcac ggttcgtgta
     2041 attgctccgc cccctcagtc cgagcattca agctggcaca ttttggcatc atctgtaacc
     2101 catgccccca gaattaactg ccagttctcg agagagccag tcgaaaaatc atcgtagaaa
     2161 atagtgtctc tctcgaatcc ataaagctgc gaggggacag caaggcttgc aattaataga
     2221 atgccgaatg ccagggcaga aaaacgcctg agcatcaatt cctcctgcaa tggtgagtat
     2281 ttcaatctgc tgtttggctt aatataaagc
//
LOCUS       DSEB01000094            5987 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000889, whole genome shotgun sequence.
ACCESSION   DSEB01000094 DSEB01000000
VERSION     DSEB01000094.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5987)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5987)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5987
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000889"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(38..119)
                     /locus_tag="ENO07_01705"
     tRNA            complement(38..119)
                     /locus_tag="ENO07_01705"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(83..85),aa:Tyr,seq:gta)
     gene            complement(223..295)
                     /locus_tag="ENO07_01710"
     tRNA            complement(223..295)
                     /locus_tag="ENO07_01710"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(260..262),aa:Thr,seq:tgt)
     gene            497..1435
                     /locus_tag="ENO07_01715"
     CDS             497..1435
                     /locus_tag="ENO07_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDS00711.1"
                     /translation="MIEVRNLTKYYGPELAVNDISFTVEKGQVVGFLGPNGAGKSTTV
                     KIITCYLAPTRGTCVVNGYDIFEDSLEVRKQIGYLPENTPLYSDMNVFDYLKFCVDMR
                     KDGLRRTKRVGEVIEICGLKDVLQKDIGELSKGFRQRVGLAQAMIHDPQILILDEPTV
                     GLDPNQIVEIRNLIKDLGREKTVILCSHILPEVEATCNRVLIINKGEIVADGTADELG
                     ASAKGKGRLVLKLKDAPLEKIENIFNVSGIDRVAEVSAAEPGVSTVVLEVLKGADPRE
                     NIFRYCVEHDLILLEMSRERVSLESVFRQLTSGEGV"
     gene            1437..2147
                     /locus_tag="ENO07_01720"
     CDS             1437..2147
                     /locus_tag="ENO07_01720"
                     /inference="COORDINATES: protein motif:HMM:PF13346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HDS00712.1"
                     /translation="MTAVWVLTKKELRSYFSSPIAYIVAVLFLGILGFFYSNSIFMSK
                     SADLRALFSIAHIIYLVFIPAISMRLISEEKRSGTIEVLGTMPLTDWQLVLGKFIPAF
                     ILMLITLLFTLINYFTVSFLGDPDTGVVLGGYLGLVLLAAAYLSIGIFTSSLTSNQIV
                     AFITSFTVILLFFVLNQLKFYVGGFLASILEFISSDYHFQSIQRGVIDSRNVIFYVTI
                     TFLFIFLTVQVLQSRKWR"
     gene            2147..3757
                     /locus_tag="ENO07_01725"
     CDS             2147..3757
                     /locus_tag="ENO07_01725"
                     /inference="COORDINATES: protein motif:HMM:PF09822.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00713.1"
                     /translation="MAKAVKTKSAAVVNILIVVLILVVVNLLSINIFARWDLTEENIY
                     SISEPSKKIISSLDDRLTVKVFFTEDLPAPHNTDRRYLKDLLDDFRAYSNGNMVYEFV
                     EDPLTENRQEASSYNLQPVQFNVMGSTTAEQKLGYKALVLIYGGENEKIPFINNMEMF
                     EYDFIRLVKNLSERGKTRVGFTFGHGELPLDGQLTIAKQILQEDFEVVPIDLRKVPEI
                     PGDVEALFIVAPSERFSDRALYVLDQYIMRGGKVGFFLNRFKMNQNMGTIDRVDTGLD
                     SLLRAYGVGVKQNFAIDQNCYTYMDLRRVEGGFMPVNVKVPFFININNFNEENLVTKY
                     QKTMSLIGASTLDTSVQIPEGVEREILFTTSEKSGTISENIQARLQQIDDLDYDQFYL
                     PLAAVLSGQFESYFADKFVPAADSADTAFVPPAQEKLNRAIENSRLLVVGSGNFFDDQ
                     AARDRSRRFQQNFVFFRNIADWLAQDDELITIRSKGNIYSPFTRVIDRDIETTVKLIN
                     IFAMPVVIIIFGLIRWQVKRSAKRSRAV"
     gene            3754..4698
                     /locus_tag="ENO07_01730"
     CDS             3754..4698
                     /locus_tag="ENO07_01730"
                     /inference="COORDINATES: protein motif:HMM:PF14238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HDS00714.1"
                     /translation="MRRILLPLVILLVLIAAYLYVQNRETESIAPEKIEEYISFDMEN
                     VDSVVVEKPQDTLKFYRLDDRWRIVVDSRPRPADTTQVKQVVEMISSIDVGSLVSQNP
                     EKQHLYRVDSLNGTLISVYGQDQELARFYLGKNASNYNYSYIRKAGSTDVYMAEDVMS
                     YRFDKAAEDWRDKSIIQVDTATLVSIVFQYSDQEFSLERRDSLWFISGDGVSGEVKAS
                     ADTVELLKRLVANLRAEDFYQPADSSFVDLENPELRLTFNHIDGTRDVLHVLGGNDSN
                     TRYYVRLPEQEEPYVVLFAKYSGLTRKPNNFITTEKNG"
     gene            4934..5788
                     /locus_tag="ENO07_01735"
     CDS             4934..5788
                     /locus_tag="ENO07_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00715.1"
                     /translation="MRAFKPVATEPSFFITIILIMSLIIACSSDETEERGEISEGESQ
                     QALEQQRPKFTDAGLADPGYEGWKQYFDEFVIVYFPPDSALQERVPAIATTIKRVVME
                     NAYRLHVRPPTPFVFYLYNNTTEVEQLTDCSNTCVRGNVIHYMTLTPLGQPIMIRLLP
                     EFDPDGTPYKFVYEGIVTLLDYSRRNYVQRAYIDLYNGELPSMATLLENDEYMAVDSA
                     TRVQAAASFLQYLTQPPWAPDSVLSLYKSDKAPDQALLDIFGRTPQDLEEGWHEYLRE
                     NSGLDVEY"
     gene            5775..>5987
                     /locus_tag="ENO07_01740"
     CDS             5775..>5987
                     /locus_tag="ENO07_01740"
                     /inference="COORDINATES: protein motif:HMM:PF00285.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HDS00716.1"
                     /translation="MWSIDMAEESWKTSITDIGPGKIRVRGFDVPDLMENASFAEVVF
                     LVLKGELPSEPEARMMEAILVSSVDHG"
BASE COUNT         1532 a         1390 c         1511 g         1554 t
ORIGIN      
        1 gaactgtaaa ggatgtgccc ggaatatacc aatcaaatgg agggggtagg attcgaacct
       61 acgaaggctt cgccgacaga tttacagtct gttccctttg gccactcggg aacccctcca
      121 taaatttctt ccggaccaga agaccggttc aaagttcagc aaatatacga ttttaccagc
      181 ttctagcaag cggattttga accgcctgaa taataatatc aaagccggcg aagggactcg
      241 aacccccgac tggctgatta caaatcagct actctaccag ctgagttacg ccggcctgac
      301 tcgttaaatt agtttccaac cccccattgt caagctcttt aattctatta tctgaacttt
      361 ttacgcaacc ttcagtccgg catttgatac acataatatt ttttttggaa ccaaaaattc
      421 cattattcag taaaatttct taataaaaaa atgcttgatt ttttggtcag gttttataca
      481 attacaattt catgttatga tagaagtacg aaatctgaca aaatattacg ggcccgagct
      541 ggcggtaaac gatatctcct tcaccgtcga aaaggggcag gtggtcggtt tcctgggtcc
      601 caacggcgca gggaaatcga cgacggtcaa gatcataacc tgctacctgg ccccgacccg
      661 cggtacatgc gtggtcaacg gctacgatat cttcgaggat tctctcgagg tccgcaagca
      721 gatcggctat cttcccgaaa atacaccgct ttattccgat atgaatgtct tcgattacct
      781 caagttttgt gtggatatgc gcaaggacgg attaaggcgc acaaagcgtg tgggcgaggt
      841 aatcgaaatt tgcggcttga aggatgtgct ccagaaagat atcggggaat tatccaaggg
      901 cttccgtcaa agagtgggtc tggcgcaggc catgattcat gatccccaga ttcttattct
      961 ggatgagccc acagtcgggc ttgatccgaa ccagattgtg gaaatacgga atctgatcaa
     1021 ggacctcgga cgcgagaaga cagtcattct ctgctctcat attctgcccg aggtggaggc
     1081 gacctgcaac cgtgttttga ttataaacaa gggggaaatc gttgccgatg gcaccgccga
     1141 tgagctgggg gcctcggcca aaggcaaggg acgccttgtt ctgaagctga aggatgcccc
     1201 attagagaag atagaaaata tttttaatgt ctcagggatc gacagggtgg ccgaggtttc
     1261 tgcggccgag cctggtgttt ccactgttgt gctggaggtg ctcaaaggcg ccgatcccag
     1321 ggagaatatt ttcagatatt gtgtcgaaca tgatctgatt ttgctggaga tgagcaggga
     1381 aagggtttca cttgaatccg tattccgtca actgaccagc ggggagggag tataagttga
     1441 ctgctgtctg ggtactgaca aaaaaagaac ttagatcata cttcagctct cccattgcat
     1501 atatcgtagc tgttctgttt ctgggaattc tgggcttttt ctactccaac agcatattca
     1561 tgagcaagag cgccgatctc cgggctctct tttcaattgc acatatcatc tatctggttt
     1621 tcataccggc gatttccatg agactgattt cggaggaaaa aagatcgggg accattgagg
     1681 tgctcggcac catgccactc accgactggc agctggtgct gggaaaattc attccggctt
     1741 tcattctgat gttgataacg ctcctgttca ccctgataaa ctatttcacc gtctcgtttc
     1801 tgggagatcc ggatacggga gtggtgcttg gcggctatct gggcctggtt ctgctggctg
     1861 cggcctacct ttccataggt atcttcacat caagcctgac aagcaaccag atcgtggcat
     1921 tcataacgtc atttacagtg attctgctgt tcttcgtgct gaatcaattg aagttctatg
     1981 tgggcggatt tctggcctcg atactggaat ttatttccag cgattatcat ttccagtcca
     2041 tacagcgcgg cgtcatcgac agccgtaacg tgatatttta tgtcactata actttcctgt
     2101 tcatctttct gacagtacag gtattacaaa gcaggaagtg gcgataatgg caaaagcggt
     2161 taagacaaaa tcggcggcag ttgttaatat attgatagtg gttctgattc tggtggtggt
     2221 caatctgctc tcgattaata tcttcgccag gtgggacctg accgaggaga atatttactc
     2281 tatctcggaa ccatcaaaaa aaatcatctc cagccttgat gacaggctta cggtcaaggt
     2341 ttttttcacc gaagatctgc cggcgccgca taataccgac aggagatacc tgaaagatct
     2401 gctggatgat ttcagggcct attccaacgg caatatggtc tatgagtttg tggaagatcc
     2461 cctgaccgaa aacaggcagg aagcctcaag ttacaacctc cagccggtgc agttcaatgt
     2521 catgggctcc accaccgccg aacagaaact gggatataaa gcgctggtgc ttatttacgg
     2581 cggggaaaat gagaagattc ctttcataaa caatatggag atgttcgagt acgacttcat
     2641 tcggctcgtg aagaacctca gcgaacgtgg aaagactcgt gtgggattca ccttcggaca
     2701 cggggagctt cctctcgacg gtcagcttac catcgccaaa cagatccttc aggaggattt
     2761 cgaggtagtt ccgattgatt tgaggaaggt gccggagatt cccggcgatg tcgaggctct
     2821 ctttatagtg gcgccctcgg agcggttttc cgaccgtgcc ctgtatgtcc tggatcagta
     2881 tataatgcgt ggcggaaaag tcggattctt tctcaatcgc tttaagatga atcaaaatat
     2941 gggcacgatt gacagagtcg ataccgggct ggactccctg ctgcgtgcct atggggtcgg
     3001 ggtaaaacag aatttcgcga ttgatcagaa ctgttacaca tatatggatt tgagacgagt
     3061 cgagggcgga tttatgccgg tcaatgtcaa ggtgccgttc tttatcaaca tcaataattt
     3121 caatgaagag aacctggtca ccaaatatca gaagactatg agcctgatag gtgcaagtac
     3181 tctggataca tcggtacaaa tccctgaggg tgtggagcgg gaaatacttt ttaccacctc
     3241 ggagaaatcg gggacgatca gcgaaaacat tcaagcccgg ctccagcaga tagatgattt
     3301 ggattatgat cagttctatc tccctctggc ggcagtctta agcggtcaat ttgaatccta
     3361 ttttgccgat aaatttgtgc ccgcagcgga ttcggccgac acagcttttg taccgcctgc
     3421 ccaggaaaag ctcaaccggg ccatagaaaa ttcccgtctg ctggtagtgg gaagcggcaa
     3481 tttctttgac gatcaggcag cccgcgaccg cagccggaga ttccagcaga attttgtatt
     3541 cttccgcaat atcgccgact ggctggccca ggacgatgag ctgattacaa tccgttccaa
     3601 gggcaacatc tacagtccct tcacaagggt catcgacagg gatatcgaga caacagtcaa
     3661 gttgatcaat atattcgcta tgccggtggt gataataatt ttcggactga tcaggtggca
     3721 ggttaaacgt tcagccaaaa ggagcagagc ggtatgaggc ggatacttct acctctggtt
     3781 attcttttag tccttatcgc agcatacctg tatgtgcaga atcgtgaaac cgagtcgatc
     3841 gcacccgaaa aaatagagga atatataagc ttcgacatgg aaaatgtgga cagcgtcgtg
     3901 gtggagaagc cccaggatac actgaagttc tacaggctcg atgacagatg gcgcatagtc
     3961 gtcgattcca gacctcgccc cgcagacact acccaggtca aacaggttgt cgagatgatt
     4021 tcgagcatag atgtcggaag tcttgtatct cagaatcccg aaaagcagca tctttaccga
     4081 gtcgattccc taaacggcac tctgataagc gtttacgggc aggatcagga gctggcccgc
     4141 ttctatcttg gcaagaatgc atcaaattat aattattctt acattcgcaa ggctggatcg
     4201 acggatgtct atatggcaga agatgttatg agttaccgtt ttgataaggc cgccgaggac
     4261 tggagagata aatcaataat ccaggtcgat actgccaccc ttgtatcgat tgtcttccag
     4321 tattctgatc aggagttctc gctggaaagg cgcgacagtc tctggtttat aagcggggac
     4381 ggggtctcag gtgaagtcaa ggcatccgcc gatacggttg aattgttaaa aaggctggtg
     4441 gccaatttga gagctgaaga tttctaccag cccgccgact cgtcctttgt cgaccttgaa
     4501 aatccggaac tgaggctgac cttcaaccat attgatggca cccgggatgt cttgcatgta
     4561 ttgggtggca acgactcaaa cacccgttat tacgtgcgtt tgccggagca ggaagagccg
     4621 tatgtggtgc tgtttgccaa atattcgggc cttacccgga agccaaataa tttcatcaca
     4681 accgaaaaaa atggttgaca aaaatagacc tgttatatat taatgaattg cccttcatac
     4741 acagccctct tatcaggcgg tcggcttggc atctgactgg tgctgtaatt ccgaccgctt
     4801 tttttatttc cccggatatc tgcgatgcgg tttgcgggca actatatgat tctaatctgc
     4861 ttgactttac tggccaatag tctataatta aacattcaac attcaaaaaa cttagttatt
     4921 tggaatattt gatatgagag cattcaagcc cgtggcaact gagccatctt tctttattac
     4981 aattattttg atcatgagcc tgatcattgc ctgttcatcc gatgaaacgg aggagagggg
     5041 ggagatatcc gagggcgaga gccaacaggc gctagaacag cagagaccga agttcacgga
     5101 tgcgggactt gctgatcccg gttatgaagg ctggaagcag tattttgatg aattcgtgat
     5161 cgtctatttt cctcccgatt ccgctctcca ggaacgcgtc ccggcgatcg caacgacaat
     5221 caaacgggtc gtgatggaga acgcctaccg gctgcatgtc cggcccccga cgccgtttgt
     5281 cttctattta tataataaca ccaccgaggt cgagcagctt acggattgca gcaatacgtg
     5341 tgtgcgcggc aatgtgatcc actatatgac tctcacacct ctgggccagc ctatcatgat
     5401 aaggctgctt ccggagtttg atccggacgg cacgccgtat aaatttgtct atgagggcat
     5461 agtgaccctg ctcgactact cccgcaggaa ttatgtccag agggcctata ttgaccttta
     5521 caacggcgag ttgccgtcga tggcgactct tctggaaaat gatgaataca tggcagtcga
     5581 ttccgccacc cgtgtccagg ctgcggcctc ctttctgcaa tatctgaccc aaccgccctg
     5641 ggcgcccgac agtgttctct cgctttataa gagcgataaa gcgccggatc aggcattgct
     5701 ggacatattc gggcgcaccc ctcaggacct ggaagagggc tggcatgaat atttgagaga
     5761 aaacagcgga ttagatgtgg agtattgata tggcagaaga aagctggaaa acaagtatta
     5821 ccgatatcgg cccgggcaaa atccgggtgc gcggttttga tgttcccgat ttgatggaga
     5881 acgcatcctt tgcagaggtc gtgttccttg ttctgaaagg tgaactgcct tccgagcccg
     5941 aagcgcgcat gatggaagcg attttggtat catcggtgga tcacggc
//
LOCUS       DSEB01000095            2275 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1007600, whole genome shotgun sequence.
ACCESSION   DSEB01000095 DSEB01000000
VERSION     DSEB01000095.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1007600"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..728
                     /gene="otsB"
                     /locus_tag="ENO07_01745"
     CDS             <1..728
                     /gene="otsB"
                     /locus_tag="ENO07_01745"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:PF02358.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HDS00717.1"
                     /translation="HKSDKRLFLLDYDGSLKHLVDEPMEAKPDDEVLEILSTITADEK
                     NELVIVSGRDSETLEEWFGHLNSGMIANHGAWIKHKGQEWEMLEPLDNIWMKNIRPIM
                     QLYVDRTPGAQLEEKEFSLVFHYRRAVPEMAQVRVSELKDDLRAMTTSLNLGILEGSK
                     VIEIKNTNINKGNSALRFISKAEWDFVMAIGDDWTDEDMFMVLPGNSYSIKVGLVPSN
                     AKYHLDDVDAVREFLKSLLGLNR"
     gene            complement(725..2155)
                     /gene="lpdA"
                     /locus_tag="ENO07_01750"
     CDS             complement(725..2155)
                     /gene="lpdA"
                     /locus_tag="ENO07_01750"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDS00718.1"
                     /translation="MADKNKSIKLAIVGGGPGGYAAAFLAADLGMQVTLIDPEKNPGG
                     VCLYRGCIPSKTLLHVAKVVNDAADAKEWGVSFNKPGFNAKKIRGWKDEVVEKLTGGL
                     GELGRLRKVEHVRGTAKFKNGTTLIISKKDGSTDSMSYDKIIIATGSEPTVIPGLDID
                     SPNLLNSTTALDLENIPKSMLVIGGGYIGLELGTVYAALGTKVSVVEMTPSLLPGVDK
                     DMVSVLSKRLKGMFESIMLNTTVSEMREQKNGIKVSFEGQGAEKKAGIYEKVLMAVGR
                     KPKSKDLGLENTQVEVDDKGFIKVDQTRRTSDPNIYAIGDVAGEPMLAHKATHEGRVA
                     AEVIAGHDVIYEPRAIPAVVFTDPEIAWCGLTQTEARGKGIDVKIVKFPWAASGRALT
                     LGRNDGLTKLVVDPETERILGVGIAGAGAGELIAEGVLAVEMAATVTDVGMSIHPHPT
                     LSETVMEAAELFHSHSTHYWRPKRKS"
     gene            complement(2185..>2275)
                     /locus_tag="ENO07_01755"
     CDS             complement(2185..>2275)
                     /locus_tag="ENO07_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00719.1"
                     /translation="HRIIDGADAARFLKWVSEALEHPLLLDFE"
BASE COUNT          559 a          611 c          536 g          569 t
ORIGIN      
        1 atcataaaag cgataaacgc ctgttccttc tggattatga cggttcattg aaacacctgg
       61 tggatgagcc aatggaggcc aaaccggatg atgaagtgct ggagatactt tccacaatta
      121 cagcggatga gaagaatgaa cttgtgattg tcagcggacg cgacagtgag acgctggaag
      181 aatggttcgg ccatctcaat tcgggcatga tagcgaatca tggcgcctgg atcaaacaca
      241 agggccagga gtgggaaatg ctcgaaccgc tcgataatat atggatgaag aatatcaggc
      301 cgattatgca gctttatgtc gatcgtacgc ccggcgcgca gctcgaggaa aaagaatttt
      361 cgcttgtgtt tcactataga cgagcggtgc ccgaaatggc gcaggtgcgg gtaagtgagc
      421 tcaaggatga tctcagggct atgactactt ctctgaacct agggatcctg gaaggcagca
      481 aggtaataga aattaaaaac accaacataa ataagggcaa tagcgcactg cgttttatct
      541 caaaagctga gtgggatttt gtcatggcca tcggcgacga ctggactgat gaggacatgt
      601 ttatggtgct gcccggcaac agctattcta tcaaggtcgg cctggtgccg tccaacgcca
      661 agtatcatct cgatgatgtc gatgcggtac gcgaattcct gaaaagcctg ctcgggttga
      721 accgctagct ctttcttttg ggcctccagt aatgagtgct gtggctgtga aaaagctcgg
      781 cggcttccat aacagtctct gaaagagtgg gatgcggatg gattgacata ccgacatcag
      841 tcacggtcgc ggccatctca actgccagga caccttcagc aatcagctct cctgctcccg
      901 ctccggcaat tccaacgccc agaattcgtt ctgtctccgg atcgaccacg agcttggtca
      961 ggccgtcatt acgacccagg gtcaatgctc ttcctgaggc tgcccatggg aatttcacta
     1021 tcttcacatc gattcccttg cccctggcct cggtctgggt aagtccgcac caggcgattt
     1081 caggatcggt gaacaccacc gccggtatgg cacgaggctc atagatgacg tcatgacccg
     1141 ctataacctc ggccgcaacc cgaccctcat gggtggcttt gtgggcaagc atcggctccc
     1201 cggccacatc cccgatggca taaatattgg gatcactggt cctgcgtgtc tggtcgacct
     1261 tgataaagcc cttatcgtca acctcaacct gcgtattttc caatccgagg tccttgctct
     1321 tcggcttcct gcctaccgcc atcaagactt tttcgtatat gcccgctttt ttttccgcgc
     1381 cctggccctc gaaagacacc ttgataccat ttttctgttc tctcatttcg gagacagtcg
     1441 tattaagcat gatagattca aacatcccct tcaatcgctt tgataaaaca gagaccatgt
     1501 ctttatcgac gcccggaaga agactcgggg tcatctcgac cacagaaact ttggtaccca
     1561 gcgcagcata aacagtcccg agctcaagac cgatatagcc tcccccgatt accagcattg
     1621 atttcggtat attttccaga tcaagcgcgg tcgttgaatt gagcaaattg ggcgaatcta
     1681 tatccagacc cggtataacc gttggctccg atcctgtagc aatgatgatc ttatcatagg
     1741 acatcgaatc ggtacttcca tctttcttgg agatgataag agttgtgccg tttttgaatt
     1801 tggcggtgcc cctgacatgt tcgacctttc ggagcctgcc gagttcaccc agtccccctg
     1861 tcaatttttc gacaacctcg tctttccagc cgcggatctt ttttgcattg aacccgggtt
     1921 tgttgaacga cacaccccac tccttcgcat cggcggcatc attaacgacc ttagccacat
     1981 gcaaaagagt ttttgagggt atacatccgc gataaagaca tacgccaccc ggattcttct
     2041 ccggatcaat caaggttacc tgcataccca gatcagcagc aaggaatgct gctgcatatc
     2101 cgcccggacc gccgccgaca atcgcaagtt ttattgattt atttttatca gccataaaat
     2161 tctccgattt tatccatcca acaattattc aaaatcaagc agcaagggat gctccagggc
     2221 ttcgctcacc cacttcaaaa atcgggcggc atccgcacca tcaataattc tatga
//
LOCUS       DSEB01000096            7923 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000494, whole genome shotgun sequence.
ACCESSION   DSEB01000096 DSEB01000000
VERSION     DSEB01000096.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7923)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7923)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7923
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000494"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(57..467)
                     /locus_tag="ENO07_01760"
     CDS             complement(57..467)
                     /locus_tag="ENO07_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDS00720.1"
                     /translation="MVENPSDDKIKEILETKKVVAVVGLSPRPERASNDVASYLKKKG
                     YKIIPVNPGHEEILGEKAYPSLSDIPEKVDIVDVFRRPEHTGPIIDEAISIDADVIWL
                     QQGIRNDEAARKALDAGKTVVQDRCMYQEHRRLM"
     gene            complement(479..796)
                     /gene="trxA"
                     /locus_tag="ENO07_01765"
     CDS             complement(479..796)
                     /gene="trxA"
                     /locus_tag="ENO07_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDS00721.1"
                     /translation="MPKHVTEKDFQQEVIESDIPVMVDFWAEWCFPCKAIAPVVDELA
                     KEYQGKIKFVKVDVDSNNRIAADYMIMSIPSLLIFKGGKVVDTIRGAVPKDTIQKHIE
                     KVV"
     gene            complement(1078..5265)
                     /locus_tag="ENO07_01770"
     CDS             complement(1078..5265)
                     /locus_tag="ENO07_01770"
                     /inference="COORDINATES: protein
                     motif:HMM:PF01869.18,HMM:PF09989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA activase"
                     /protein_id="HDS00722.1"
                     /translation="MPMNMNYYLGIDIGSVALKVVAVSGDGQIKYSDYRRLYGQPGDV
                     LKEALVDLGKEVSAHQIAGLVSTGNAGELAHKVIGGDFANEVLSVSKAAQYLTPEVNT
                     MIEMGGADSKLLCFNRLGNQLALSDFAMNSLCAAGTGSFLDQQANRLDISIENEFGEM
                     ALKSKNPPRIAGRCSVFAKSDMIHLQQIATPDYDIVAGLCFAVARNFKSAIARGKKIK
                     KPVAFVGGVASNAGMVRAFEHILEMHTGELIIPDEHRIFCAYGAAMIAKEKDQTQPFD
                     LLACEKHAGNMLHASVSTRERLEYSFPEQKHYKTTLTLKRGPEPKLTEGYLGVDIGSL
                     STNLVVIDKNKNVLARRYLMTAGRPIEAVRKGLAEIGEELQNTVKIVGCATTGSGRYL
                     IGDFIGADIVRNEITAQATAAIMLDRKVDTIFEIGGQDSKFISIDDGVVVDFEMNKAC
                     AAGTGSFLQEQAEKLGIKIDEEFGDRALKAACPVGCGERCTVFMESDLNSYQQAGAEK
                     NDLVAGLAYSIAKNYLTRVVGKRRVGEHIFFQGGVAWNKGVVAAFEKILGKPVHVPPH
                     HDVTGAIGAALIAMDRPGEHASKFRGFDLSTRNYTVEPFICEDCSNMCEIRKVTIEGE
                     EPLYYGSRCEKYDTEVKAKDDRFDFIKFRNKVLYSSIEKTKLEEKAVTIGFPRVLIFY
                     ELYPFWASFFRKLGYKVVLSAPTNQKILSYSLENFSAETCFPIKVVNGHVKDLLDKQV
                     EYIFLPSIINVREDSAPHENSFLCPHIQSIPYTIMANFDLDKYHSKFINVPVSLELTE
                     DALKHELKDLQKQFGWTRDKFEAAIEAGLRNQREFYDKIAEKGREFLETRDKSKPAIC
                     IISRPYNGCDAKLSLEIPKKLLGLGVDVMPMEALPLSEKYGELADANMYWRFGQKILA
                     AAEIIRDIPNLYPVYITNFGCGPDSFITHFFQERLGGKPYLQIEIDEHSADAGIVTRL
                     EAFLDSLESKQAIERFRKKVYISRFSSNGCPRTLYIPYMSDHAIPFAAAFRACGIPSE
                     ALPEPNENTLEFGKKFTSGKECFPCQVTTGDIIGKVQSGDFDRNASAFFMPSASGPCR
                     FGQYHLYQRQVLDELGYEDVPIISPNSRTGYDDPAFGSDDFKRIGWRAVVATDLLFKV
                     YHHFRPRLEDKTEIDGLYRKYLHKLADYIEHRRDLEPLFIAALSDFKKFSSGNGNKLP
                     IIGVVGEIFLRANRFSNNFLVEHLENLGAQVWLAPMAEWIFYTNFTYRRRLKKENSLG
                     EFLTANLTNFVQHKDEKRLVSLVAEEIPSAYDPPVEETIKLAQPYIDVSLSGEAILSV
                     GKAIEYVHNGAGGIVNTLPFMCMPGTIVSGVAGKVADDLGGVPWLNMAYEGLSDKGDE
                     LRLEAFVHQARQFSASR"
     gene            complement(5305..7512)
                     /locus_tag="ENO07_01775"
     CDS             complement(5305..7512)
                     /locus_tag="ENO07_01775"
                     /inference="COORDINATES: protein
                     motif:HMM:PF01578.18,HMM:PF16327.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00723.1"
                     /translation="MIILGNALLVLTGLACFATVLLFGLAAFKHKSYEVPANNLFAIF
                     ALLVVIMAALFLSQIINHNYELEYVYSYSSNSLGFFLLMSTFWAGQVGTFLLWLLFAA
                     IAGMFLLKVNWEHKNRLMFFYMLTAVFLVVLLFVKSPFKPLNPEAIGMPAEMFPPQDG
                     RGLNPLLQNFWMVIHPPIVFVGYTLLAIPFAYALAALSARDYSTWSKKVFPWSLLSGS
                     VLGLGIFLGGYWAYETLGWGGYWAWDPVENSSLIPWLTNLALIHGLLLERSRGSLRKT
                     NLLLAVLGYILVLYGTFLTRSGVLQEFSVHSFVAPGKMLYWVMVVFMAGFTILSAIYF
                     IRSLPSLKRPKEEKEKISEPQQWFETLVILGALFTILLAVLTWVGTSSPLITTILGNP
                     SNVTQSFYNAIAMPIGIIMAVLAAISSLYIYYTHKLRYFVIRLVISLAIAVIGTLIAY
                     FLAIRSVSILVLIFASFFAIVSNIFAIFSMPRKNIINMAGNITHVGFGLILIGFIASS
                     VFSQSTGVIKLEADQNKELLGMHLKFLGIGDNFMSSDNRVRVAVSNGDDEYIARPAFY
                     QDPYTRQTVINPYIRKTLFHDIYFAPQRYLPDYTTLTLGKGETNSAFGYDITFEEFQI
                     GSHEHTGAMKVATVLNVSVDGKEHQVMPTFHPDPSAADNPGYASIPDTDYKIYVDRIF
                     ADEGKIMIDVVGVGDLVLEVTRKPFINLVWFGAVIIVVGTFLAYLKRRKLAQL"
     gene            complement(7509..7916)
                     /locus_tag="ENO07_01780"
     CDS             complement(7509..7916)
                     /locus_tag="ENO07_01780"
                     /inference="COORDINATES: protein motif:HMM:PF03100.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HDS00724.1"
                     /translation="MPKRVIIVLIIVVAFGAFMFSSLVRSCSPHVSFEKARSGSNVQI
                     FGKINSGEVVFDSTDLSLSFTLLNEEGEAMPVVYHGVVPSNFEYAEEATCIGSWQNGK
                     FRADKLLLKCPSKYEGEVEESEQYEFYNEDQSI"
BASE COUNT         2043 a         2088 c         1835 g         1957 t
ORIGIN      
        1 ctattataca taattgacgg cgagtagtct atagtacaaa aaaattattt caaaaactac
       61 atcagtctgc gatgttcctg gtacatgcac ctgtcctgca ccaccgtttt cccggcatcg
      121 agcgccttcc gtgcggcctc gtcgttgcgg atgccctgtt gcagccaaat cacatcagca
      181 tctatactta tggcctcgtc gataattgga ccggtatgct ccggacggcg aaaaacatca
      241 acaatgtcaa ctttttccgg gatatcggag agggagggat aagccttctc gcccaggatt
      301 tcctcatgcc cgggatttac cgggataatc ttgtatccct tttttttgag ataactggcg
      361 acatcattgg aagccctctc cggccgggga gataatccca cgaccgccac cacttttttg
      421 gtttccagaa tttcttttat tttatcatcc gagggattct cgaccatgct gctacccttt
      481 aaaccacttt ttcgatatgt ttctggattg tatccttggg cactgctccg cgaatggtat
      541 ccaccacttt accgcccttg aaaatcagca gcgaggggat ggacataatc atataatcgg
      601 cggcgatgcg gttgttgctg tcgacatcaa ccttaacaaa cttgattttg ccctgatatt
      661 ctttggccag ctcatcaaca accggcgcaa tcgccttgca cgggaaacac cattcagccc
      721 agaaatcaac cataactgga atatctgatt cgattacctc ttgttgaaag tctttttcgg
      781 tcacatgttt tggcatcttt cctcctactt atatttatct cggccgatca gctccaggac
      841 ctgtcgtgag ggagcccggt caatctgctt tctatgttaa tccgatcagt aacctaaagt
      901 ctaaatcccg ccttactttg ctatacgccc ttgcccaaca taacacccgg ccgaagcatt
      961 ttgttcacaa acattcacgc cggaaattat caagccatta aaatacaacc tgccccggtt
     1021 gcgcaattac tgaatataga cataggctct ttcaaatcaa taaaaatttt gcaggaatca
     1081 tcgagaggcg ctgaattggc gggcctgatg cacaaatgcc tccaacctga gctcatcgcc
     1141 cttatcgctc aagccctcat aagccatgtt gagccatggc actccgccta aatcatcggc
     1201 cacctttccg gctacgccgg agacaatggt gcccggcata cacatgaacg gcagggtatt
     1261 gacgatgccg ccggcgccat tgtggacata ctcaatcgcc ttcccgactg aaagaattgc
     1321 ctcgcccgag agggaaacat caatgtaagg ctgagccagt ttgatagttt cttccaccgg
     1381 gggatcatag gccgagggta tttcctcggc cacaaggctt accagcctct tctcgtcctt
     1441 atgctgcaca aaattggtca aattggcggt caggaattca cccaggctgt tctctttttt
     1501 gagcctccgg cggtaggtga agttggtata gaatatccac tcggccatcg gcgccagcca
     1561 cacctgtgcc cccagattct ccaggtgctc gaccagaaag ttatttgaga aacgatttgc
     1621 acgcaggaat atttccccta ccactccgat tattggaagc ttatttccat taccggaact
     1681 aaatttctta aagtccgaca gggcggcgat aaagagtggc tccagatctc tcctgtgctc
     1741 aatataatcc gccaatttat gcagatattt ccgatagagt ccatctattt cagtcttgtc
     1801 ttccaggcgg ggcctgaaat gatgatagac tttaaatagc agatcggtgg caaccaccgc
     1861 cctccatcca attctcttga aatcatcaga gccgaaggcc ggatcatcat atcctgtgcg
     1921 cgaattcggt gaaattatgg gcacatcctc ataccccagc tcatccagca cctgccgctg
     1981 atagagatga tactgcccga aacggcaggg acccgaagcg gagggcataa aaaatgcgct
     2041 ggcattgcgg tcaaaatcac ccgattgaac cttgccgatg atatccccgg tggttacctg
     2101 gcatggaaag cattccttgc cggaggtaaa cttcttgccg aattcaagtg tattctcatt
     2161 tggttcaggc agagcttcag agggtatccc gcaggcccgg aaagcggcgg cgaacggtat
     2221 cgcatgatcg gacatatagg gtatatacag agtccgcgga cagccattac tcgagaaccg
     2281 cgaaatatag accttcttcc tgaatcgctc aatcgcttgt ttgctttcca ggctgtccag
     2341 gaaagcctcg agacgcgtga caatacccgc atcggccgaa tgctcatcga tttcaatttg
     2401 cagatatggt ttgccgccca ggcgttcctg gaaaaagtgc gtgataaatg aatcggggcc
     2461 gcagccgaag ttggttatat aaacagggta aaggttggga atatccctga taatttcagc
     2521 cgcagccagt atcttctgcc cgaagcgcca gtacatattg gcatcagcaa gctcgccgta
     2581 tttctccgat agcggcagcg cctccatagg catcacatcc acacccagac ccagcagctt
     2641 cttcggtatt tccagcgaaa gcttggcgtc acagccgtta tatggacgcg atataatgca
     2701 gatggccggt ttggatttat ctctcgtttc gaggaattcg cgtccctttt cagcaatctt
     2761 atcataaaat tctctctgat ttctgagacc cgcttctatt gccgcctcga atttgtcccg
     2821 ggtccagcca aattgcttct gcagatcctt caattcatgt ttcagcgcgt cttccgtcag
     2881 ctcgagtgaa acgggcacat taataaattt gctgtgatat ttgtccagat caaaatttgc
     2941 catgatggta tatggtatgg actgtatatg cgggcagaga aaactgttct catgcggtgc
     3001 ggaatcctcg cgcacgttta tgatagaggg caggaaaata tactcgacct gtttgtcgag
     3061 cagatccttg acatgcccgt taacaacctt aatcgggaag caggtctcgg ctgaaaaatt
     3121 ctcgaggctg tacgacaata tcttttgatt ggttggcgcc gacagtacga ccttgtaacc
     3181 cagcttacgg aagaacgagg cccagaatgg atagagttcg taaaaaatca ggacccgggg
     3241 aaagcctatg gtcacagctt tctcctcgag tttggtcttt tctatggatg agtacagaac
     3301 cttatttctg aatttgatga aatcaaaacg atcatccttg gccttgacct cggtatcgta
     3361 tttctcgcag cgggaaccgt aatacaacgg ctcttccccc tcgatcgtta cctttctgat
     3421 ctcgcacatg ttggagcagt cttcacagat gaacggctca accgtgtaat ttcgagtgga
     3481 gagatcaaag ccgcggaact ttgatgcatg ttcgccggga cgatccatag cgataagcgc
     3541 ggccccgata gcgccggtaa catcatgatg aggagggaca tgcaccggtt tgcccagtat
     3601 tttctcaaag gccgcgacca ctcccttgtt ccaggccacc ccgccctgga aaaagatatg
     3661 ctcgccgact cttcttttgc caaccacacg ggtcagatag ttcttagcga ttgaatatgc
     3721 cagacccgcc acaagatcat ttttctcggc cccggcctgc tgataggagt tgagatcgga
     3781 ctccataaaa accgtgcagc gttcgccgca tccgaccggg caggcagcct tcaaagcccg
     3841 gtctccgaat tcctcatcaa tctttatacc cagcttctcg gcctgctctt gcaggaatga
     3901 tccggtaccc gcggcacagg ctttattcat ctcgaaatcg accaccacgc catcatcgat
     3961 tgatatgaac ttggagtcct gtccgccgat ctcgaagatc gtatctacct tgcggtcaag
     4021 cattattgcc gcggtagcct gggcggtgat ctcattgcgg acaatatcgg cgccgatgaa
     4081 atctccgatc aggtaccgtc ccgagcctgt agtggcgcat cctacaattt ttacagtatt
     4141 ctgaagttcc tcgccgattt cggccagccc cttgcgcaca gcctcaatcg ggcgtccagc
     4201 ggtcatcagg tagcgccgtg ccaggacatt cttattttta tcgataacaa ccagattagt
     4261 ggaaagtgag cctatatcca ctcccagata accctcggtc aattttggct caggaccgcg
     4321 cttgagagtc aaggtggtct tgtagtgctt ttgctcaggg aatgaatatt ccagcctttc
     4381 tcgagtgctc actgaagcgt gcaacatgtt accggcgtgc ttttcacagg caagaagatc
     4441 aaacggctgg gtctgatcct tttccttcgc aatcatggcg gcgccataag cacagaatat
     4501 gcgatgttcg tcgggtatta tcaattcacc cgtgtgcatc tccagaatat gctcaaacgc
     4561 tcttaccatt cccgcattgg aagccacccc gcccacaaat gctacgggtt tcttgatttt
     4621 cttgccgcgt gctatggccg atttaaaatt gcgcgccaca gcaaagcaga gaccggccac
     4681 aatatcataa tcgggtgtgg cgatctgctg cagatgtatc atgtcggatt tggcgaaaac
     4741 cgaacatcgc cccgcgatgc ggggaggatt tttggatttc aatgccatct caccgaattc
     4801 attctcaatc gatatatcca gcctgttggc ctgctggtcg aggaatgatc cggtgcctgc
     4861 cgcacagagg gaattcatgg caaaatccga gagcgccagc tggtttccga ggcggttgaa
     4921 gcagagcagc ttggagtcgg ccccgcccat ctcgatcatc gtatttactt ctggtgtaag
     4981 atattgagca gccttggaaa ccgagagcac ttcattggcg aaatcaccgc cgatcacttt
     5041 gtgggccagc tctccggcat taccggtgga gaccaggccc gcaatctggt gggccgagac
     5101 ttctttgcca agatcaacaa gggcttcctt gagtacatct cccggctggc catacagtct
     5161 gcgatagtcg gaatacttaa tttgcccatc gcccgagaca gccaccactt ttaaggcaac
     5221 agagccaatg tcgattccca aatagtaatt catattcatg ggcatgaatt aactcaacag
     5281 gaacgattaa tcaatgattt tttattataa ctgtgcaagt ttacgccgct tcagataggc
     5341 aagaaaagtc cccacgacga ttatcactgc accgaaccat actaaattaa tgaatggctt
     5401 gcgggtgacc tcaagcacaa gatcgcccac acccacaaca tctatcataa tcttgccttc
     5461 atcggcaaaa atgcgatcga catatatttt atagtcagta tctggaatac ttgcataacc
     5521 gggattatcc gcagcagacg gatcaggatg gaacgtcggc attacctgat gctccttccc
     5581 atctactgag acattgagta cagtagcgac tttcatagct ccggtatgtt catggcttcc
     5641 gatctggaat tcctcgaagg tgatatcata accgaaagcg ctgtttgtct cccccttgcc
     5701 cagggtcaag gtcgtataat cgggcaggta cctctgggga gcgaagtata tatcatggaa
     5761 cagcgtcttt cgaatatagg gatttatcac tgtctggcgc gtgtaagggt cctggtaaaa
     5821 agcaggtctg gcaatgtatt catcatcgcc attactgacc gccaccctca ccctgttatc
     5881 gctgctcata aaattatcgc cgataccaag aaatttcaga tgcatcccca gaagctcctt
     5941 attctggtcg gcttccagtt tgatcacgcc ggtgctctga gagaataccg aggaggcaat
     6001 gaatccgatc aggatcagtc caaagcctac atgagtgata ttcccggcca tattgatgat
     6061 gttctttctg ggcatggaaa aaatggcaaa gatattggag acaattgcaa agaatgaggc
     6121 aaatatcagc accaatatcg aaaccgatct gatcgccaga aaatatgcta tcaatgttcc
     6181 gattacagct attgccagcg agataacgag ccgaatcaca aagtacctga gcttgtgagt
     6241 gtaatatata tagagcgagg atatcgccgc cagaacagcc atgataattc cgatcggcat
     6301 cgctatggca ttataaaatg actgggtgac attggaaggg ttccccagga tcgtcgtaat
     6361 cagaggcgag gatgtgccca cccaggtcaa aacggccagc aatatggtga agagagctcc
     6421 caggatcacc agggtctcga accattgctg gggctccgaa attttctctt tctcctcttt
     6481 gggccttttc agtgacggca gactgcggat gaaatagatt gcagaaagaa ttgtaaaacc
     6541 agccataaag acaaccatga cccagtaaag catcttgcct ggggccacaa atgaatgcac
     6601 cgaaaattcc tgcaagactc cgctgcgggt aagaaatgta ccatacaaga caaggatgta
     6661 tccaagcaca gccagcagca gattagtttt gcgcaggctc ccgcgcgatc tttccagtag
     6721 aagtccatgt atcagcgcca ggtttgtcag ccagggaatc agagatgagt tttcgaccgg
     6781 gtcccaggcc cagtagccgc cccatcccag agtctcatag gcccagtagc cgcccagaaa
     6841 tattcccagt cccagcactg aacctgagag caggctccag ggaaatacct tcttggacca
     6901 ggtgctgtaa tccctggccg ataaagctgc cagggcatag gcgaacggta tcgccagtaa
     6961 agtatagccg acgaacacaa tcgggggatg aatcaccatc cagaaattct gcaatagcgg
     7021 attcaatccg cgcccgtcct gaggcggaaa catctccgcc ggcatcccga tagcctccgg
     7081 atttagaggc ttgaagggcg acttgacaaa tagcagcaca accaggaaca ccgccgtaag
     7141 catgtagaag aacatcaggc gattcttatg ctcccagttg actttcagca ggaacatccc
     7201 cgcaattgcg gcaaataaaa gccagagaag gaatgtgccc acctgtccgg cccagaatgt
     7261 ggacatcagc aggaagaaac ccaggctgtt ggacgagtac gaatagacat actccagttc
     7321 ataattgtga ttgataatct gggacagaaa aagggcggcc atgattacga caagcagtgc
     7381 aaatattgca aaaagattgt tggccgggac ctcgtatgat ttatgcttga atgccgccag
     7441 accaaacaac agcacagttg caaagcaggc cagtccggtc aggacaagca atgcattacc
     7501 cagtattatc atatggattg atcctcgttg taaaactcat actgttcgct ctcttcaact
     7561 tccccctcgt atttggaggg acatttgaga agcagcttgt ctgccctgaa tttgccgttc
     7621 tgccatgatc caatgcatgt ggcttcctcg gcatactcaa aatttgatgg taccacgccg
     7681 tgatagacaa ccggcatagc ctcgccctct tcattcaata aagtaaacga aagtgagaga
     7741 tcggtactgt cgaagacaac ttcaccgcta ttaatcttgc cgaaaatctg cacattgctg
     7801 ccggatctgg ccttttcgaa gctgacatgc ggcgaacatg accgcaccaa agaggaaaac
     7861 ataaacgcac cgaaggccac tactatgatc aaaactataa ttactctctt gggcacctac
     7921 ttc
//
LOCUS       DSEB01000097            4988 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001327, whole genome shotgun sequence.
ACCESSION   DSEB01000097 DSEB01000000
VERSION     DSEB01000097.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001327"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(445..1437)
                     /locus_tag="ENO07_01785"
     CDS             complement(445..1437)
                     /locus_tag="ENO07_01785"
                     /inference="COORDINATES: protein motif:HMM:PF05036.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00725.1"
                     /translation="MFTRIFTAGILLLLLMPSLSSGDWGDIRNLIDQGKLRQAEEELG
                     HELTGTYSDWQGLYLSGIIKANGELSVSELERSLLLCEDKCGQIVSRLCAAYYSFGDY
                     RKLVEVYDDNKDKIDDDGRSFGAYWFASLAYLKLGDFNRAEDVIKKAGEVNKRYAVWA
                     ELLRANFQFMMDKRGDARDRLNGVIRAGGTAGFSALYARTYQYAMSGNIDKAYSGFTM
                     LKEAHEKFIGAPELLRLLQSESTTSADGTAEKIAGVSYTIQIGTYADRAEMQRMRSSL
                     ENDGWTTFLMSKFVGGKKYWILTVGSFDTVEGAQGSKKILEGALDASLKVIILE"
     gene            complement(1444..2493)
                     /locus_tag="ENO07_01790"
     CDS             complement(1444..2493)
                     /locus_tag="ENO07_01790"
                     /inference="COORDINATES: protein motif:HMM:PF13432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDS00726.1"
                     /translation="MLDVLVWILVIAVLALIGVVFYIYKSTPRVKLTPHETYLEALKA
                     LLNNDKGKALLRLKETVMADTNNIDAYLRLTSLLREKGNARRALQVDADLNLRQNLSQ
                     YERAEVMLSLAEDYMLLSQYDAAEKVLQELKKFPERRAAAASKLVKLFTRKKDWRKAV
                     EYQEEYLKHQDIDDKSSLAELKLKHGHELKSNEKFHEARVEYRSALKYNPNLAEAVIA
                     IGDSYEKEGKLENAVKAWKKIIDVDASKAGEVFNRIQKVLFDLGQYGEIEEFYNQVLE
                     KDPKNLQSLLGLASISEKRGDGVVAEDYYNQVLEMNPEYMPALLGLIRFYQRQQRTDD
                     AARVINKTAQTLLKD"
     gene            complement(2497..2817)
                     /locus_tag="ENO07_01795"
     CDS             complement(2497..2817)
                     /locus_tag="ENO07_01795"
                     /inference="COORDINATES: protein motif:HMM:PF06305.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HDS00727.1"
                     /translation="MWLVRLFFIIIAIAVLVGFILYNPDEPVNLHFPWGDYYNVQLIF
                     VCAVAFIIGMFVTFIYSVFYYLKIAGDIREKNRQIKNLEVELSALRNRSLEDLEEAPE
                     EREE"
     gene            complement(2879..3589)
                     /locus_tag="ENO07_01800"
     CDS             complement(2879..3589)
                     /locus_tag="ENO07_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00728.1"
                     /translation="MKRLFLITLGILLLASSTNLLAGDKAGEVIDDSVYHDFTYDFTI
                     KVADGWEVARIKDADDIYRVTISKVSPVMPVKYNENPHLFTYPKLTVMADTTTVSLDS
                     LETLLSAREGKVDIVKQAIKDFGLLTYSKYRPEFEPSVTIKYKDFEGLILPARKQMDI
                     SDYRRGTIYFLQNDEITLIIEAVAELERFSFNDRAFGDMVRSIRVGREEPKEEEAVEI
                     IQEEEVEGEEDKKNEEKE"
     gene            complement(3695..>4988)
                     /locus_tag="ENO07_01805"
     CDS             complement(3695..>4988)
                     /locus_tag="ENO07_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HDS00729.1"
                     /translation="TILEDFSGKYLAAVDTGSVARVFSLGADWERRELMGIGGGDRLR
                     DFALNDNRAYIAAGADPLIQMDTRTYGYRPAITFYPARSEHLVRHDSLLFLADNNNYQ
                     LWVSEINLSSWLSTHNLIQYDGKAVALSSDRGADGSINLALFTRDKIILHNLNQDNMQ
                     INSTAEISGLEDITTGLIQGEYLYAADGDLLTIYIIADPARPVFRGEYEWPGEIRAVQ
                     IIGDRIYTSGDFGLQINQLQGPEPWILEGSFEFDGRIEDFVLDNNHALCAAGDDGLLI
                     LDTDSGNGIELAESYESPGYARAIDISDSSLFICDGYGFLIYDISYSGEFPSPDPDPV
                     PRRFELSHNYPNPFNQSTLIAVDIPSAADQTRIEIDIFNILGQRVRRLVDEVAIPGRK
                     IYRWNGRDDEGLEISSGIYFYRCRLGGIEESRKMLLLK"
BASE COUNT         1231 a         1352 c         1064 g         1341 t
ORIGIN      
        1 ccatataatt ttcgattcaa tacaggtcgt gtatcctgca aactgtaaaa ttccgcaagt
       61 aaacggtatt atttcaatac cttaccggtt aaacctaact gattaaaccc atcaagtcaa
      121 caaaattgga aatatgggct gaaaactgaa tagtctgcgg attttacagg gccaatctgc
      181 gcgatgagtc aatgcatctc caggggcaag cgcacgacac gttgtaccct tatctaattc
      241 gctccaaaca tctgtatcgc ccttgtggtc gtggacgatc tggtaacaca ttgatagtcc
      301 cgggccgagg ccgacccaca tctggattgg tctcgtaatt tgatgtcggt gaggttgaag
      361 gcaatatact gtcctctgcc gaaatttcaa taattatatt gtggaaatag ataaatgtgc
      421 agttgtgtga gaaatgatta aatcttattc cagaattata accttaagag aggcatccag
      481 ggcgccctcc agtatctttt ttgagccctg cgctccctcc acagtgtcga atgagcccac
      541 cgttagtatc caatattttt ttccgcccac aaacttgctc atcagaaatg tcgtccagcc
      601 gtcgttctcc agtgagctgc gcatcctttg catttcagcc ctgtcggcat aagtgccgat
      661 ctgtatggta tagctgactc ccgctatttt ttcagcggtg ccgtcggctg aagtcgtgct
      721 ctcactctga agcagccgca atagttcagg ggccccgata aacttctcgt gcgcctcttt
      781 cagcatcgta aacccggagt aggctttatc tatatttccg ctcatggcat actggtatgt
      841 tcgcgcatac agggccgaga atcccgccgt ccctccggcc cggattactc cattgagcct
      901 gtctctcgcg tcccctcttt tgtccatcat gaattgaaaa ttagcccgaa gcagttccgc
      961 ccagaccgca tagcgtttgt tgacttcacc cgcttttttg atgacatcct ctgccctgtt
     1021 gaaatcccca agtttcaaat aagccagtga tgcaaaccag tatgctccaa acgacctgcc
     1081 gtcatcatca atcttatcct tattgtcatc ataaacctcg actaatttac gataatcgcc
     1141 aaaggagtaa taagccgcgc aaagccgcga gactatttgc ccgcatttat cctcgcataa
     1201 aagcagcgat ctttccagtt cactgacaga aagttctccg ttggctttga tgatacccga
     1261 caggtatagt ccctgccagt cggaatatgt gcccgtcagc tcatgcccca gctcctcctc
     1321 ggcctggcgc agcttgccct ggtcgatcaa attgcgaata tccccccaat ccccgctcga
     1381 tagggagggc atcagaagca gtaacaatat cccggcagta aaaatgcgag taaacataga
     1441 gaattaatct ttgagtaatg tctgggcggt cttgttgatc acgcgcgccg catcgtcggt
     1501 acgctgctgt ctctggtaaa accgtatcaa ccccagcagc gcaggcatat attcgggatt
     1561 catttccaga acctggttgt agtaatcttc ggccaccacc ccgtcaccgc gcttttcaga
     1621 gattgaggcc aggcccagca aagactgaag attcttcggg tccttctcca gaacctgatt
     1681 gtaaaattct tcaatttccc cgtattgccc cagatcgaag aggactttct gaatccgatt
     1741 gaagacctcc cccgctttgg aggcatcgac atcgatgatc tttttccagg ccttgaccgc
     1801 attttcgagt ttgccttctt tttcatagga gtccccgata gcgatcacag cttccgccag
     1861 attgggatta tatttgagcg ccgaccgata ttccacccgc gcctcatgga acttctcatt
     1921 acttttcaat tcgtggccgt gtttcagttt gagctcggcc agtgaactct tatcatcaat
     1981 atcctggtgc ttgagatact cttcctgata ttccaccgcc ttgcgccagt ccttcttgcg
     2041 cgtaaaaagc ttcaccagct tgctggcagc ggcggctctg cgctcgggga atttcttcag
     2101 ctcctgcagg accttctcgg ccgcatcata ctgagacaag agcatataat cctcggccag
     2161 cgaaagcatc acctcggccc tctcatattg cgataggttt tggcgcaggt tcagatcagc
     2221 atcaacctga agggccctcc tggcgttgcc cttctcgcgc aacagactgg ttaaacgtaa
     2281 ataggcgtca atattgttgg tatcggccat caccgtctct ttcagcctca ggagcgcctt
     2341 acccttatca ttattcagaa gagccttcaa tgcctccaga taggtctcat ggggagtgag
     2401 ctttaccctg ggagtacttt tatatatata aaagacaacc cctatcaaag ccagtacggc
     2461 tattaccagt atccacacca atacgtcgag caaaatctat tcctcccgct cttcgggcgc
     2521 ctcttccaga tcttccaggg acctgtttcg caacgcagac agttcaacct cgagattctt
     2581 tatctgcctg ttcttctcgc gaatgtcgcc ggctattttg agataataaa atacggaata
     2641 tatgaaggtc acgaacatcc cgataatgaa agccacagca cagacgaaaa taagctggac
     2701 attgtagtaa tccccccagg gaaaatggag attgaccggt tcatcgggat tataaagaat
     2761 aaagccaact aaaactgcaa tagctattat aatgaagaac aatctaacta accacatagc
     2821 ctcaccccgt taagttttta ccattctacg ctgttgacag ttggctgtca aacaaaattt
     2881 attccttctc ttcattcttt ttatcttctt cgccctcaac ctcttcttcc tgtataatct
     2941 cgacagcttc ttcttctttc ggctcttccc ggccgacccg gatcgaccgc accatatctc
     3001 cgaaagcgcg gtcattgaag ctgaagcgtt ccagctccgc aaccgcttca ataatcagcg
     3061 taatctcgtc gttctgcaga aaatagattg ttccacgcct gtaatctgag atatccatct
     3121 gcttgcgggc cggcaggatc aatccttcga agtccttgta cttgatcgtg accgagggct
     3181 cgaattccgg cctgtacttt gaatatgtaa gaaggccgaa atccttgata gcctgcttca
     3241 ctatatcgac tttgccttca cgggcactca gcagtgtttc aaggctgtca agcgagacag
     3301 tggtcgtatc ggccattacg gtaagtttgg gatacgtgaa caggtgcgga ttctcgttat
     3361 atttcaccgg cataaccggg ctgactttgg agatggtgac ccggtagata tcgtctgcat
     3421 ccttgatcct ggcgacctcc cagccatctg ctacctttat tgtaaaatca taggtgaaat
     3481 catggtagac actgtcgtca ataacctcgc ccgctttgtc tccggcaagc aggtttgtcg
     3541 atgacgccag gagcagaatt cctaatgtaa tcaaaaacag tcttttcatg ataccctccc
     3601 gttttttaat aacttagaaa agttattggg gtatgtcaat cagtttacac atttacaaaa
     3661 acttctacgc ctggagggcc gcccggttcg caggctattt caaaagcagc atcttgcggg
     3721 attcctcaat cccccccaga cggcagcggt agaaatatat tccgctcgag atttcaaggc
     3781 cctcatcatc tcggccgttc cagcggtata ttttgcgccc gggaatggca acctcgtcca
     3841 ccagccttct caccctttgc cccagaatat tgaaaatatc gatctcgatt ctcgtttgat
     3901 ctgctgcgga tggtatatcc acagcaatta aagtagattg gttgaaggga ttgggatagt
     3961 tatgggaaag ctcgaatcgc ctcggcacag ggtccggatc aggagatgga aattcgccgc
     4021 tgtagctgat atcatatatc aagaatccat agccgtcgca gataaacaat gagctgtcgg
     4081 aaatatcaat tgcgcgggca taaccgggag attcataact ttcggcaagt tcaatcccat
     4141 ttccggaatc ggtatcgaga atcaggagac catcatcacc ggctgcacaa agagcgtgat
     4201 tattatcaag cacaaaatcc tcaatcctgc cgtcgaattc gaatgagcct tccagaatcc
     4261 acggctccgg tccctgcaat tgatttatct gcaggccgaa atccccggag gtatatatcc
     4321 tgtcaccgat gatctgaacc gccctgatct cgccgggcca ttcatactcc cctctgaaaa
     4381 cgggcctggc cgggtcggca attatatata tagtgagcaa atccccatct gccgcataga
     4441 gatattctcc ctgaatcagc ccggttgtaa tatcctccag gccgctgatc tcggcagtac
     4501 tgtttatctg catgttgtcc tgattcagat tatgcagtat tatcttatcc ctggtgaaca
     4561 gtgccaggtt tatacttcca tctgctcccc ggtcagagga taaagccaca gccttcccgt
     4621 catattgtat cagattatgg gtggagagcc acgaagacag attgatttca ctgacccaga
     4681 gctgataatt attattatct gccagaaaca gaaggctgtc gtgtctcaca agatgttcgc
     4741 tgcgggcggg ataaaaagtt atcgccggac ggtagccata cgttctggta tccatttgta
     4801 tcaaagggtc tgctcccgca gctatatagg ccctgttatc attcagggca aaatccctca
     4861 gccgatctcc acctccgatc cccatcagtt cacgtctctc ccagtcagcg cccagactaa
     4921 atacccgggc cacacttccg gtatcgactg ccgccaggta tttacccgaa aaatcctcta
     4981 atatagta
//
LOCUS       DSEB01000098            7902 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000498, whole genome shotgun sequence.
ACCESSION   DSEB01000098 DSEB01000000
VERSION     DSEB01000098.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7902)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7902)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7902
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000498"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            317..2566
                     /locus_tag="ENO07_01810"
     CDS             317..2566
                     /locus_tag="ENO07_01810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HDS00730.1"
                     /translation="MKRILIIFACAIFLVAYQPALAGNGTGDNPGGSTDDTSPSDELN
                     FDIFGIFESIMTQIMAGAQAYEGSGLAPSPKITDSMGCVNVDVNLPDSVSANSPFDST
                     LTTGSLLIYNCDTQSAQIQLAFAFTASIDNFIDTTIVFDNIFINLEAGDSVYCEFIFP
                     VPPFNATYTFCVTAITSGGAGMTDCATMVVSGQNMPDIPYFAPGFLFQGEDCVLFMEL
                     GDSNLALIVQNLGPFGPGDTVFVVGTLVDDCGIQCTGAGGCVIDNTIDSLPSPPPPPP
                     FEACGVLIQGANCVLFTPMGFGDTAFVLDNYGIFEVGDTVSVSGELEIGCETECTGAM
                     GCILSNSIDTCGSSPPPPPPTFAGCGALVQGTNCVLFSPDFYGDTLFTLANYGTFGVG
                     DSVFVEGEIYPGQDSVCVDSWGHINNIIITLCQDSIPPIFAGCGYLYEDQGCIIFQPL
                     DQPNGYLLYNYGDFGPGDTVFVDGEVVDCGTVCNVVGCIDNPIIDSCTAPPPPTIAEC
                     GVIIETPDCPLFVPFTWFNSYFVLSDYGGYSPGDSVFVSGIISELPDTLDCSGPVGYI
                     QVDSIGPCGGSPGPQYSGIGYLGYEGSCLVYYDLYEYYTSYVLENYGDFVAGDTVYVD
                     GTFGVDCDFNCSDTALCLFDNTIILANPPDSIPVSDRVKEIKNVRSYPNPFNPIAIIS
                     FNIPVQSRVTLEIYNILGQKVETLIDGKILQGDQEYPWNGDRFASGIYFYRIETEYDV
                     VTKKMILNK"
     gene            complement(2618..3220)
                     /locus_tag="ENO07_01815"
     CDS             complement(2618..3220)
                     /locus_tag="ENO07_01815"
                     /inference="COORDINATES: protein motif:HMM:PF02643.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HDS00731.1"
                     /translation="MSKKKNIKDKDKKELKTKPRINQRLIYLIFGIAVVAAFLISQFL
                     PWPAEKKPANKAIRNQDRSSVFDFRKEGEVKFLDVDGELKVEIDVEIADDPGEITLGL
                     MYRDDLDSRQGMLFIFEDEQPRSFWMRNTILPLDMIFADSSGKIVTIHEHTVPYSKES
                     YISTIPAQYVVEVNAGFVNSHNINIGDKIEWRRDNREQKN"
     gene            complement(3289..4155)
                     /locus_tag="ENO07_01820"
     CDS             complement(3289..4155)
                     /locus_tag="ENO07_01820"
                     /inference="COORDINATES: protein motif:HMM:PF01297.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation ABC transporter substrate-binding
                     protein"
                     /protein_id="HDS00732.1"
                     /translation="MKSVHIYIAILALLFLPGKSSASQETEVFVGIPPLKYLVEQIAG
                     DQVECHVLLKPGESPHTFEPTPRQMIDLSQSSFYFALGFPFESRITEKLGRDGKPGII
                     DIAEGTERRQVHSEAGTDEHHPHTDTDDPHVWLSPENVRIMARNIERVLEDYDPSDSL
                     VYRNRLKDFLHELDSLDRIIDSLISPYRRSTIMVFHPAFGYFTDYYGLDQIAVETDGK
                     SPSPKQIESIIERARESGIRVIFVQPRFDPKSAESIAGAIDGKVVPIDPLENNLLQNL
                     LDIAEKIRDALK"
     gene            complement(4912..6330)
                     /locus_tag="ENO07_01825"
     CDS             complement(4912..6330)
                     /locus_tag="ENO07_01825"
                     /inference="COORDINATES: protein motif:HMM:PF00082.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00733.1"
                     /translation="MANTSKRYRAVLGVIVPLLGIIMAGHAIAESTASQQVIVEIGVG
                     KSAQEAAAAINGTVIDSIPGKLIYLLSFPDSQELSQTLTTLYENANVDTAQPNHIVGF
                     TEIDQVDQVIPDQSAPPLLQGTSPANYYGHAGYDAIGADSAHKVSTGENIIVAIIDNG
                     IDFEHPLFNGAFECNGYDFVDDDPLPAEDSGLYYGHGTFVAGIIMRVAPDCKLIPYRA
                     FDEDGSGSVFNITRAVYRAIEDSARVLNMSFSMTSDNLILKDAIDAAFDANIAMAAAP
                     GNDSSYVSVYPADYRGVIAVSAIDSMDLISDFSNYGDLIDICAPGEDIYSSLAGSYQW
                     GTWSGTSFSAPMAAGVSALILSLEPNLTTSRVEQVLAGTAETELVWGTVVPDDSLYGH
                     GRLDALEAVSEFNRGDVDCSGEINAIDKAYLQDYIYQYGPAPIPLPKVGDLDCSGEVD
                     LTDYMILINYLENGGPKPTPCE"
     gene            complement(6344..>7902)
                     /locus_tag="ENO07_01830"
     CDS             complement(6344..>7902)
                     /locus_tag="ENO07_01830"
                     /inference="COORDINATES: protein motif:HMM:PF12770.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HDS00734.1"
                     /translation="SGDTENAFRISRSLSRKNLVSYQRYNLDYLMGQCYFRMGDYSRA
                     LEKFRTAVIIVEKMLSGLYPDELRFFFAIDKHDCYKMVVECLLRLKRIDDSFLANLRA
                     LESINYSASHEIKSNKSVPGELIEQRNSLRAALKKLNQVKHAEQRHLEKSGSYLSLEQ
                     KLWSNERKIRSILYPPEVRGQDEGINLRDLQNSLESDQAVLNYFSSGSSIGAFLADNA
                     KIQYIEFDISLSELEYYLQKLHFVFESAVFGLKSEGNGRIAESYLSYMYEHIFRPLSR
                     ETDKRKLIIIADGSFAQIPFGALRDETGNLMTDRYQIRMAVNPNSLRSREPSRHGFGN
                     KRNAVFAISSDSLPSTGAEANRIKQAFEGAHLYIDERAASSNLLDEIKKADGFIHIAA
                     HASRSSENPLFSRILMGDGPFFPFDLFQSGIAAELVTLSGCQTAAPGLYYGNSFSLAK
                     SFFQAGAKYVLATLWPVSDKISMLFMGRFYDSLKHKQNIFSAYLDAVNEIKGITENPA
                     FWSSFILLGI"
BASE COUNT         1973 a         1963 c         1798 g         2168 t
ORIGIN      
        1 gctgcgggga tatcaatcag gatcagagta tcaacataag tgacgcagtt tatatcataa
       61 attatgtatt tcttcccgat tgtccgcctc cccctgatat aaactctgcc gatgtaaacg
      121 acgatggggc cgtcaatttg agcgatgcaa tttgcctggt caactacata tatctcggcg
      181 gccctgagcc caattgcccc gaatgatata aatactcttc aaaaaaagag tattttacaa
      241 agccgaatat cgcacctgaa gagtagaaac aagatttatc ttcaagtgtg aattttctaa
      301 gcagagggag agcattatga aaaggatttt gatcatattc gcctgcgcga tttttctggt
      361 ggcttatcag cctgccctgg cgggtaacgg tactggagat aatcccggag gaagtactga
      421 tgataccagt ccatcggacg agctgaattt cgatattttc ggtattttcg aaagtatcat
      481 gacccagatc atggcaggtg cccaggctta tgagggctcc gggctggcgc cttcaccaaa
      541 aataacagat tccatgggct gtgttaatgt agatgtgaat cttcccgatt ccgtttccgc
      601 caatagccca tttgattcaa ctcttacgac cggatccctc ctgatttaca actgcgatac
      661 tcaatcggcc cagatacaac tcgcttttgc ctttacagcg agcatagata attttatcga
      721 tacaaccatc gttttcgaca atattttcat taatctggag gccggtgatt cggtctattg
      781 tgaatttata ttccccgttc ccccgttcaa tgcaacctat actttctgtg tgactgccat
      841 cacaagcggc ggcgccggca tgacagattg tgcgaccatg gttgtttccg gacagaacat
      901 gccggatatt ccctactttg ccccgggatt tcttttccag ggcgaggatt gcgtgctttt
      961 catggagctg ggtgattcca acctcgcttt aatcgtccaa aacctggggc cctttggacc
     1021 gggagataca gtattcgtgg ttggcacact tgttgatgat tgcggtatcc aatgcaccgg
     1081 ggccggagga tgcgtgatcg ataacactat tgactcgctg ccgtcaccgc ctccaccacc
     1141 tccctttgag gcctgcggtg tattgattca gggagcgaat tgcgtgctct tcacccccat
     1201 gggtttcggt gacaccgctt ttgttttgga taattacggc attttcgagg tcggcgatac
     1261 tgtgtctgtc agcggtgaac ttgaaatcgg ctgtgaaact gaatgtaccg gtgccatggg
     1321 atgtatactc agtaatagca ttgatacatg cggctcatcc ccgcctccac cgccgcctac
     1381 attcgccgga tgcggtgcgc ttgttcaggg aactaattgc gtgctcttca gtccagactt
     1441 ctacggcgac acgcttttca cgctcgcaaa ttatggcaca ttcggtgtgg gggattccgt
     1501 gtttgtggaa ggagaaatct atcccgggca ggattctgtc tgcgtagatt cctggggaca
     1561 tatcaacaat attataatta ctctctgcca ggacagcata ccgcccatct ttgccggatg
     1621 cgggtatctt tatgaggatc agggatgtat cattttccaa cccctggatc aaccgaacgg
     1681 ctatctcctt tacaattatg gtgacttcgg ccccggcgat acggttttcg tggacgggga
     1741 agtggtggat tgcggcacgg tttgtaacgt agtcggttgc atagataacc cgattataga
     1801 ttcctgtacg gctcctcctc ctccgacgat tgcggaatgc ggcgtgatca tcgagacccc
     1861 tgattgtcct ctgtttgtgc ccttcacctg gttcaattcc tacttcgtgc tgagcgatta
     1921 cggcggctat agccccggcg acagtgtatt cgtttccggt atcatctctg aattgccgga
     1981 taccctcgat tgttccggtc ccgtcggata tatacaggtc gactcaatcg gtccctgcgg
     2041 cggcagtccc ggaccgcaat attccggaat cggctatctc ggatatgaag gaagctgcct
     2101 ggtttactat gatctgtatg aatactatac ctcttacgtc ctcgaaaatt acggcgactt
     2161 cgttgccggc gatacggtct atgtggatgg tactttcgga gttgattgcg atttcaactg
     2221 ctccgacaca gcgctttgcc tgtttgataa cactatcatc ctggcaaatc cgccggattc
     2281 cataccggta agcgatagag taaaggaaat taaaaatgtc agaagttatc ccaatccctt
     2341 taatccgata gcaataatat ctttcaatat tccggtacaa tcaagggtaa cgctggagat
     2401 ttataatatt ctcgggcaga aagtggagac cctgattgac ggcaaaatcc tgcaaggtga
     2461 tcaggagtat ccgtggaacg gcgatagatt tgccagcggg atttacttct acaggatcga
     2521 gaccgaatat gatgtcgtga cgaagaagat gattctgaat aaataaagga taggcttttc
     2581 agaggaaggg gaggtgaatt ttccgattca tctcccctta gtttttctgt tctcggttat
     2641 cccgcctcca ttcaatcttg tcgccgatat ttatattatg gctgtttaca aaaccggcat
     2701 taacttcaac cacatattga gcgggaattg tggatatgta tgattctttc gaatatggta
     2761 cagtgtgctc atgaatcgtt acgattttcc cgctgctgtc agcaaatatc atatccagcg
     2821 gaagaatggt gtttctcatc cagaaggaac gcggctgctc gtcctcaaat ataaacagca
     2881 tgccctgacg ggagtccaga tcatcgcgat acatcaagcc cagtgtaatc tctcccggat
     2941 catccgcaat ttcaacatct atttcaactt tgagctcacc atcaacatca agaaacttca
     3001 cctctccttc tttcctgaaa tcaaataccg acgatctgtc ctgattcctt attgctttat
     3061 tggcaggttt cttctctgcc ggccacggga gaaactgtga aatcagaaag gcggctacca
     3121 ccgctatacc aaaaatcagg taaatcagac gctgattgat tctcggtttt gttttgagct
     3181 cttttttgtc tttatctttt atgtttttct tctttgacat atcaagaaag tccggttgcc
     3241 gtccacatct tattctataa ttacaaatta gcaacaagat tgtggcaatt atttaagcgc
     3301 atccctgatt ttttcggcga tatcgagcag gttttggaga agattgtttt ccagcgggtc
     3361 gatcggcacg acctttccat caattgcccc tgcaatcgat tcggcacttt ttggatcgaa
     3421 ccggggctgg acgaagatta ctcttatgcc ggattctctg gctctttcaa ttatcgattc
     3481 aatttgcttc gggctggggc tcttgccgtc ggtttccacc gcaatttgat cgagaccgta
     3541 gtagtcagtg aaatatccga aggcggggtg aaagaccata atagtggagc gtctgtaagg
     3601 tgatataagc gagtctatta tccggtcgag gctatccagt tcgtgcagaa aatccttcag
     3661 acggttgcgg taaaccaggc tgtcggaggg atcataatcc tccaggaccc gttcaatatt
     3721 ccgggccatt attctcacgt tttccgggga taaccagaca tgcggatcat ctgtgtctgt
     3781 atgcggatga tgctcgtctg tcccggcttc cgagtgcacc tgtcttctct ctgtaccctc
     3841 cgcaatgtct ataatcccgg gttttccgtc cctccccaat ttctcggtta tgcgcgattc
     3901 aaatggaaaa ccaagggcaa agtaaaacga tgactgtgag agatcgatca tctgcctcgg
     3961 agtaggttcg aaagtatgag gcgattcccc aggtttcagg agcacatgac attccacttg
     4021 atcgcctgca atctgctcga ccagatattt caggggcggt atgcctacaa atacctctgt
     4081 ctcttgagaa gcgcttgatt tgcccggcag gaaaagtaga gccaaaattg caatatatat
     4141 gtgtacactt ttcatttttt attaatcccg gcttaatgta atacgaagta atacattagt
     4201 caatattcta ttttacactg gattaaataa aaaacaccgg cgattcttag agaatcgccg
     4261 gccttaaaag atccccgcac tacatgccct ttggccacag ggggacagcc cccttctaca
     4321 acctactcct ggaggaccgt acatccccat cagctacagg actgtcttat caccaggtgc
     4381 gtcattgacc cgggataagc ttttcaaatg acggcaaccc atccaaatgg gttcggtaaa
     4441 actaagttct gcgtttcatc ccagccagat tcaaaaattt ttatgctgct gacagcaatt
     4501 ggtatggaag cagagtaagc ttgctctgtg gaattttact gctgtcagca gcgttggtag
     4561 cttcttcaat caccttccaa atgcaggagc ttgtgactcc ttcaatatga acctcttaat
     4621 tttattaacc aggggcgacc tcttcaaaac ctgatagact ccttcacctc aaaggaactg
     4681 gcattgtcca aatctgcggg ggctccggta ataaggccaa atcgctgacc gaaatccccc
     4741 ctctcagaca acctgccaga tttcatacaa cgaatctctc aaatagtaga gtacgaaaga
     4801 tgtggaaaaa tacaataatc actctttttt ttgcggtttt tgggttattt ctttgtgctg
     4861 aaggaaataa aaaaggcccc gagtgaattc ggggccgtag attctcgaga tttactcgca
     4921 gggcgtcggc ttcggcccgc cattttcaag gtagttaatc agtatcatgt aatctgtcag
     4981 atcgacctcg cccgagcagt ccaggtctcc aactttgggt aatggtatcg gagcgggacc
     5041 atattggtag atataatcct gcaaatatgc tttatctatg gcattgattt ctccgctgca
     5101 atctacatca cctctattaa attccgatac ggcttcaagg gcatcaagac gcccgtgacc
     5161 gtagagcgaa tcatccggga caacagtacc ccataccagc tccgtttccg ctgtgccggc
     5221 aagtacctgc tcaacccggc tggtggtcag gttcggctcc aggctcagaa tcagcgctga
     5281 taccccggca gccatcggag cagagaatga cgttccgctc catgtccccc attgataaga
     5341 tcctgccagt gagctgtaaa tatcttcacc cggagcgcaa atatcgatta agtcaccata
     5401 attcgagaaa tcactgatta aatccatgga atcgattgct gataccgcaa ttacacctct
     5461 gtaatctgcc ggatagacgg aaacatagct gctatcgtta ccgggagcgg cagccatagc
     5521 aatattagca tcaaaagcgg catcaatagc atccttcaaa atgaggttat ccgatgtcat
     5581 gctgaaactc atattcaaaa cccgcgcgga atcctcaatg gccctataaa ccgctctggt
     5641 aatattgaac acagaacctg aaccgtcctc atcaaatgcc ctgtacggta tgagcttgca
     5701 atcgggcgcg accctcatta tgataccggc cacaaacgtc ccgtgaccgt agtaaaggcc
     5761 ggaatcctcg gccggaagag gatcatcatc gacaaaatca tagccattgc attcaaaggc
     5821 gccgttaaag agcggatgtt cgaagtcaat gccgttatct ataatcgcca cgataatatt
     5881 ctccccggtc gaaaccttat gggcggaatc cgccccgata gcatcataac cggcgtgccc
     5941 gtaatagttt gccgggctgg tcccctgcag cagtggaggg gcgctttgat ccgggatgac
     6001 ttgatccacc tggtcgatct ccgtaaatcc gacaatgtga ttgggctgag cggtatcgac
     6061 attggcgttt tcatataatg tggtcaaagt ctgcgacaac tcctgtgaat ccggaaatga
     6121 tagaagatat atcagcttac ccggaattga atcaatgaca gttccattga ttgccgcagc
     6181 cgcctcctga gccgatttcc ccaccccaat ttctacaatc acctgctggc tggcggtact
     6241 ttcagctatt gcatgccccg ccatgattat ccctaagagc ggcacaatga cccccaaaac
     6301 agccctgtat ctctttgatg tatttgccat tatcaaactc cttctaaata cccagcagga
     6361 taaaagagct ccagaatgcc gggttttctg ttatgccctt gatttcattg actgcgtcca
     6421 aataggcaga gaaaatgttc tgcttgtgtt tcagggaatc ataaaatcgg cccatgaaga
     6481 gcatgctgat tttgtctgat actggccaaa gagtagccaa aacatatttg gctcctgcct
     6541 gaaagaacga cttggcgaga ctgaatgaat tgccataata taagcccgga gccgcagtct
     6601 gacatcccga aagtgttaca agctcagcgg caatgccgga ttgaaagaga tcgaaaggga
     6661 aaaacggccc atcgcccata agaatcctgc tgaacagggg attctcggat gagcgagagg
     6721 catgggcggc aatatggata aacccatcag cttttttgat ctcatccaga agattgctgc
     6781 tggcagctct ttcgtcgata taaaggtggg ctccctcaaa tgcctgcttg atccgattgg
     6841 cctctgctcc tgttgacggg agggaatcgg aggaaattgc aaatacagca tttctcttat
     6901 tgccgaatcc atgcctggac ggctcccttg acctgaggct gttcggattc accgccattc
     6961 tgatctgata tctgtctgtc atcaggtttc cggtctcatc cctgagagcg ccaaacggta
     7021 tctgagcaaa actcccgtct gctattatga tcaatttcct cttatccgtt tccctgctga
     7081 gcggcctgaa gatatgctca tacatataag agagataact ttccgcaata cgtccattac
     7141 cttcgctctt aagcccgaag acagcagatt cgaagacaaa gtgcagtttt tgcagatagt
     7201 attccagctc ggataaggat atatcaaatt cgatgtactg aattttcgca ttgtctgcca
     7261 gaaaggcgcc gatacttgat cccgacgaaa aatagttgag tacagcctgg tcggattcaa
     7321 ggctattctg taaatcccgc aagtttattc cttcgtcctg cccgcggact tcagggggat
     7381 aaagaatgga tcggatcttt ctctcattgg accagagttt ctgctccaga cttaaatatg
     7441 agcctgactt ttccagatgc ctttgctccg cgtgtttcac ctgattgagc tttttgagag
     7501 ccgccctgag gctgttgcgc tgttcgatca gctctccggg taccgattta ttcgatttaa
     7561 tctcatgact tgctgaataa ttgatgctct ccagcgccct caaatttgcc agaaaagaat
     7621 cgtcaatacg tttcaatctc aggaggcact ccaccaccat cttgtagcag tcatgcttgt
     7681 ctatggcgaa gaaaaacctc aattcatccg gatacaggcc gctcagcatc ttctccacga
     7741 ttataacagc ggtccggaat ttttccagcg cccttgagta gtctcccatc cggaaatagc
     7801 attgacccat cagataatca aggttgtacc tctggtagct taccaggttt ttgcgggaaa
     7861 gggatctgga aatcctgaag gcgttttcag tgtccccgga ca
//
LOCUS       DSEB01000099            5992 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000884, whole genome shotgun sequence.
ACCESSION   DSEB01000099 DSEB01000000
VERSION     DSEB01000099.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000884"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            47..337
                     /locus_tag="ENO07_01835"
     CDS             47..337
                     /locus_tag="ENO07_01835"
                     /inference="COORDINATES: protein motif:HMM:PF13174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDS00735.1"
                     /translation="MRNDYPRKTVGAAAQEYLARLYIKYEAWDDAVRALRKLPEDFKN
                     FRFANDAYFRIGAIFQNRLNQADSARVYYQKQIDAYPGSRPSELAMQSIETL"
     gene            356..934
                     /locus_tag="ENO07_01840"
     CDS             356..934
                     /locus_tag="ENO07_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00736.1"
                     /translation="MKPKQALLLLTALVFIVSSAILIAQETEVKKEVEKEVGKEDVCE
                     VKVRPTEVKNHYEFEILLTNKEPINAMSFPFYISAGEKKMYYDSVSFEGSRAEFCAVK
                     IPNPDTASQKINLGILTSMTPPIRYIDPGEGRIAKLYFTGDNGVTVNDIVVDTTFFPP
                     SNHLMGVLPDGKTQIYPKFKYTVVEEEKEEEE"
     gene            complement(1352..>5992)
                     /locus_tag="ENO07_01845"
     CDS             complement(1352..>5992)
                     /locus_tag="ENO07_01845"
                     /inference="COORDINATES: protein
                     motif:HMM:PF13448.4,HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HDS00737.1"
                     /translation="WEVFYTAPGSQAAMILEQVAASATSASSGWYDAADSSDKHELFV
                     AGDEAGDSTIFIIENADSIGFYMTPNLVDYESWYTQNIYNNDGFDHALLFPTGNPHEY
                     IVAFEDLQDGGDADYNDLVIRVRFENAAPLLSLPDDMYLNMCGQNWVCFDVAAYDQTD
                     SITLEMLEGYGSFTTMVGAGEVSDQHCFFPSGSDATYLFVFKATDNLGAFVIDSFEVV
                     YEINDRPIFSLPDDFDTVLCEYDSICVPFEAFDADGDELEIEFLQGEGTIDYVNSTFC
                     FLPAEVDFSSYTFILKASDSCCSEIETIDPDYCCPVPCPEDTITIDVRVNMVPDLVTV
                     PDFDTLLCEPQEICFPVSATDGNEGDLVTISVSSPATYNEQTGEVCFMADADGVYDIV
                     VTATDQLCGAAAEDTVTVTVELNEAPDLVAADDTSYYFCSEPYEICFENTVIDPDGPD
                     GNTFSLVSGPGAIDPQSGTVCFTPLEPGEYEFVISVTDICQNSDIDTVYISIAMNSPP
                     VLILPDDFSVVQCEYEPICIQAVADDPDLPNDVLTFIKVSGFGGIDPQTGELCFTPVV
                     AGQYDFVIRVKDLCGEVDQDTISIDVSLNSPPLMTVSPSMSVFFCEPGDSACITLSAS
                     DPDLNETLSFEQLAGPAGALDALSGEFCFELTGAGDYGFTFRVVDQCGAADTADVTVT
                     AEMNTPPVVTLPDDYSEIVCASGDEICFSASFDDLEGQDLEIAVLPFGSYSEGTVCFP
                     AAASQDTTFCIIVQATDICGATGEDTVCIDVHYNLPPTVSISPDTTIKVCQPPDNFCF
                     SYTSGDPDGTEPDITIVQGSGTVQGGQVCINIDTVGAYCVRIRATDECGEFAEDDACV
                     IFVANQPPVLTVSDDITQALCEAAEICFDNSATDPDLPDDTLTFSLTSAPQGATITAT
                     GRVCFTPAQPGTYDFTVRVTDHCGEFDEGVVSVTVELNQPPQIVADPDSLYHEYCGLG
                     APEVELCFDGLTVSDPDDDIALLTVEKTAGPGTFDAATMMTCFTAPVADTVIEFVYKV
                     SDTCEAYDLDTVLYYIYVNPECDSFTCLEVWIENTECVLTSSFVEVSISADFNIAVGG
                     FDLVVKYDPTAFTLTDVYRGDATADWEYFTYRTGDIGGCSPVCPDGLIRMVAIADRND
                     GTPHPPEEAYMPNGEIAAMRFYVTSDLNFSGLTYAVDFYWFKCSDNAFSSRTGDTLFV
                     EKLITNQYGVVWDELDDDNYPEDSRYANIGVPDSCLVGDKVKPQRCVIMRNGSICIID
                     PEEIDKRGDMNLNEIAYEIADAVLYTNYFIFGPAVFTINYGGQVAASDVNADGRTLTV
                     GDLIYLIRVLIGDATPINKLNAFAESVDLTLEKNSGEVAIKSESVSEIGIAHFVFDIS
                     EAQSLPEITASSDISDMKLEYNADGDIMRVLIYSMDTHRIPSGENVLGTIHVDGDISL
                     ISTDMADYYGSDLAVNLREVSTLPTEFMLSQNFPNPFNPETEIQLSLPEASDWSIEIF
                     NIRGQRIESFSGHSDAGVVKVLFQADNLATGMYFYRAVAGDFTDTKKMILLK"
BASE COUNT         1615 a         1566 c         1459 g         1352 t
ORIGIN      
        1 attgagcgat cagtggtata atagagcaga ggctttcttc gcgggtatgc ggaacgacta
       61 cccccggaag acagtcggtg ccgcggcgca ggaatatctg gcccggctct acataaaata
      121 tgaagcatgg gatgatgccg tgagggcatt gagaaaattg ccggaggatt tcaaaaattt
      181 caggttcgcg aatgatgcct attttagaat aggagcgatt ttccagaaca ggctgaatca
      241 ggccgattcg gcacgtgtat attatcaaaa gcagatcgat gcctatcccg gaagccgtcc
      301 cagcgaactg gcaatgcaga gtattgaaac tttataatat cttaaggagg aactggtgaa
      361 acccaaacag gcgctcttat tgctgacagc attagttttt atcgtctcat ccgctatact
      421 gatagcccag gaaaccgagg tcaaaaagga agttgaaaag gaagtgggca aggaagatgt
      481 atgtgaggta aaagttcggc ccacagaggt caagaaccat tacgagtttg agatccttct
      541 gaccaataaa gagccgataa atgctatgtc tttcccgttt tacatttcgg ccggcgaaaa
      601 gaagatgtac tatgactcag tctcatttga aggctcgcgc gccgagttct gtgccgtcaa
      661 gattcccaat ccggacaccg ccagccagaa gattaatctg ggcatactga cttccatgac
      721 cccgcccatc agatatatcg accccgggga aggaagaatt gcaaaattgt acttcaccgg
      781 cgacaacggc gtgaccgtca atgatatcgt ggtcgatacc actttcttcc cgccttcgaa
      841 tcacttgatg ggcgtgttgc cggatggcaa gacacagata tatcccaaat tcaaatatac
      901 agttgtggaa gaagaaaaag aagaagaaga ataacaattt ggaactctaa aaaagccccg
      961 cttgcggggc ttttttcttg ccatgtccgc aagccatctc attgcccaac aaagcgcaaa
     1021 gaaattaccc gcccgaatat cgccggacgg gcttctcctc cacacaatca aggataagga
     1081 atatgaaacc acgagaatca aatcctatca tagcttaaag cttatctaaa tctacagcca
     1141 gacagcgcct gccgctatca tcacgataag tgcgaagaaa atcaaaatcc agtctaaact
     1201 cataccgaac ctcctaccat atcagacagc ctatctttag acaggttaca ctccagaatc
     1261 atttttattc gatgtctgcc caaaaaaaag cgggacctct aagtcccgct cacgtcagtg
     1321 cttcttctct tcctgcagag atagagatga tttacttcag gagaatcatt ttctttgtat
     1381 cagtgaaatc acctgcgact gcccgataga agtacattcc agtcgccagg ttatcggcct
     1441 ggaacaggac ttttaccact ccggcatcgc tgtgacccga gaaactctct attcgctgtc
     1501 ccctgatatt gaagatctca atgctccaat cggacgcctc cggaagcgag agctgaatct
     1561 cggtctcagg attgaaagga ttggggaagt tctgggacag catgaattca gtcggcaggg
     1621 tcgacacttc gcgcagattg accgccaggt cgctgccgta gtaatcggcc atatcagttg
     1681 aaatgaggct gatatcacca tccacatgga tagtcccgag aacattctct cccgacggga
     1741 ttcgatgagt atccatgctg taaatcagga ccctcataat atcgccgtcg gcattatact
     1801 ccagcttcat atctgaaata tcgctcgagg ctgtaatctc gggcagcgac tgtgcttcag
     1861 agatatcgaa gacaaaatgc gctattccta tctcagaaac ggattctgac ttgattgcaa
     1921 cttcgccgct gttcttttcc agtgtaagat caacactttc agcaaaagca ttaagcttat
     1981 taatcggcgt agcatcaccg atcagcacac gaatgaggta aatcagatca ccaactgtca
     2041 atgttctgcc gtcggcgttg acgtctgaag ccgccacctg gccgccgtaa ttgatcgtga
     2101 agaccgcggg tccgaatatg aagtagttgg tgtagagtac cgcatccgca atttcatagg
     2161 caatttcatt caggttcatg tcgccacgct tgtcgatttc ctccgggtca ataatacaga
     2221 tcgagccgtt acgcattatc acacatctct gcggcttaac cttatcaccg acaagacagc
     2281 tgtcaggaac gccgatattg gcataacggg aatcctcggg ataattgtca tcatccagtt
     2341 catcccagac gactccgtat tgattggtta taagtttttc gacaaacagt gtgtcgcccg
     2401 ttctggacga gaaggcattg tcggaacact tgaaccagta gaagtcgacc gcataggtca
     2461 ggccggagaa gttcaaatcg ctggtgacat agaatctcat tgctgcgatc tcaccattgg
     2521 gcatataggc ttcttcgggc ggatgcgggg taccatcatt gcggtccgca atcgctacca
     2581 tacggatcag gccgtccgga caaaccgggc tgcagccgcc aatatcgccc gtcctgtaag
     2641 tgaaatattc ccagtccgca gtcgcgtcac cgcgatatac atcggtaagt gtaaacgcgg
     2701 tcggatcgta tttgacaacc aggtcaaatc cgccaacagc gatattaaaa tcggccgaga
     2761 tggaaacctc gacaaagctg ctggtgagca cgcattccgt gttctcgatc cagacctcga
     2821 ggcaggtgaa ggaatcgcat tcgggattta catatatata atagagcacc gtatcgaggt
     2881 catatgcctc gcatgtgtcc gaaaccttat aaacaaattc aataacggta tccgccacag
     2941 gtgcggtaaa acaggtcatc atggtcgcgg catcaaaagt acctggaccg gctgtctttt
     3001 caaccgtcag caaagcgatg tcatcatccg gatcggaaac cgtcagtccg tcaaaacaaa
     3061 gctcaacctc gggcgcaccc agaccgcagt actcgtgata gaggctgtcg ggatcggcca
     3121 cgatttgggg cggctgattc agttccactg tcacggaaac aacgccctca tcgaactcac
     3181 cgcagtgatc ggtcaccctg acagtaaagt cgtaagtgcc cggctgcgct ggcgtgaagc
     3241 atacccttcc agtggccgta atcgtagcac cctggggagc actggtcagg ctgaatgtca
     3301 gagtgtcgtc cggcaggtcg ggatcagtcg cagagttgtc gaagcagatt tcagccgcct
     3361 cgcagagcgc ctgagttatg tcatcgctca cagtcaggac aggcggctgg ttggcaacaa
     3421 atattacgca ggcatcgtcc tcagcaaatt cgccgcactc atcagtggcg cgaatccgca
     3481 cacagtaagc acctactgta tcgatattga tacagacctg gcctccctga acagtgccgc
     3541 tgccctgcac aatggtaata tcgggctcag taccgtcagg atctccggat gtgtacgaga
     3601 aacagaaatt atccggcggc tggcagacct tgatagtggt atcaggcgaa atactgacag
     3661 taggcggaag gttgtaatgc acgtcaatac agactgtatc ttcaccggtc gcaccgcata
     3721 tatcggtcgc ctgaacaata atgcagaatg ttgtatcctg ggaggcggcc gccgggaagc
     3781 agaccgtgcc ctcggaatag ctgccgaacg gcagcacggc tatttccaga tcctgaccct
     3841 ccagatcgtc aaaacttgcg ctgaagcaaa tctcatcacc ggaggcacag actatttccg
     3901 aataatcatc cgggagggtg accacaggcg gggtgttcat ttccgcggtc acggtcacat
     3961 cagccgtgtc ggccgcgccg cactgatcca caactctgaa tgtgaagccg tagtcgccgg
     4021 cgccggttaa ctcgaagcag aactcaccgc tgagagcgtc cagagccccg gccggtcccg
     4081 cgagctgctc aaagctcagg gtctcgttca ggtcgggatc ggaggcgctc agggtaatac
     4141 aggccgaatc ccccggttca cagaagaaaa ccgacattga cggcgacacc gtcataagcg
     4201 gaggtgaatt gagactgaca tctatggata ttgtatcctg gtcaacttca ccgcaaagat
     4261 cttttacccg gatcacaaaa tcatactgtc ccgccaccac aggcgtgaaa cagagttccc
     4321 cggtctgcgg atcgatgccg ccgaagcccg agacttttat aaatgtcaat acgtcattgg
     4381 gaagatcggg gtcatcggca accgcctgga tacagatcgg ctcatactca cattgaacga
     4441 cagaaaagtc atcagggagt atcagcaccg gagggctgtt catggcgatt gagatataga
     4501 ccgtgtcgat atccgaattc tggcatatat cagttacgct aattacaaat tcatactctc
     4561 ccggctccag cggtgtaaag cagacagtgc cggactgggg atcgatcgca cccggacctg
     4621 acaccaggct gaaggtattg ccgtccgggc catcggggtc gatcacggtg ttctcgaaac
     4681 aaatctcata tggctcggag cagaaataat agctggtatc gtcggctgca accaggtcag
     4741 gagcttcatt cagctctaca gtcacggtca ccgtatcctc ggccgcagcg ccgcacagct
     4801 ggtcggtggc ggtcacaact atatcgtaaa caccatcggc atccgccata aagcagactt
     4861 ctccagtctg ctcattatat gttgccgggg aggatacaga gattgtgaca agatcgcctt
     4921 cattgccgtc cgtggccgaa accgggaagc atatttcctg cggttcacag agaagcgtat
     4981 cgaaatcggg cacagttacc agatcaggca ccatgttcac cctcacgtct atcgtaatag
     5041 tatcttccgg gcacggcacg gggcagcaat aatccggatc aatcgtctct atctcagaac
     5101 agcatgaatc ggaagctttc agtataaatg tataagatga aaaatcaacc tcagccggca
     5161 ggaagcagaa ggtgctgttg acatagtcta tcgtcccttc gccctgcagg aattcaattt
     5221 ccagttcatc gccgtcggca tcaaatgctt cgaagggcac gcagattgaa tcatattcac
     5281 acaacactgt atcgaaatca tccgggagcg agaatatcgg gcgatcgttt atctcataca
     5341 ccacttcaaa agaatctata acaaatgcac ccaaattatc agtagcctta aaaacaaaga
     5401 gatatgtggc atcactgcct gagggaaaga aacaatgctg gtccgaaact tctcctgcgc
     5461 ctaccatggt tgtgaaactg ccatagcctt ccagcatctc cagtgttatc gaatcggtct
     5521 gatcatacgc ggccacatcg aaacaaaccc agttctgacc gcacatattt aaatacatat
     5581 catcggggag ggagagcaaa ggagcggcgt tctcgaacct gaccctgatc accagatcat
     5641 tataatctgc gtcacccccg tcctgcaaat cctcaaaggc aacgatatat tcatggggat
     5701 tgccggtcgg aaacaaaagt gcatggtcga agccatcgtt gttgtaaata ttctgtgtat
     5761 accacgattc gtaatcgacc aggtttggag tcatatagaa tccgatagaa tcggcattct
     5821 cgataataaa aatcgttgaa tctccggcct catcgccggc aacgaagagc tcatgtttat
     5881 ccgagctgtc ggctgcgtca taccatccgc ttgaggcgct ggtggccgac gctgccacct
     5941 gctccaggat cattgccgcc tgtgagccgg gcgcggtgta aaaaacttcc ca
//
LOCUS       DSEB01000100            1530 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1018284, whole genome shotgun sequence.
ACCESSION   DSEB01000100 DSEB01000000
VERSION     DSEB01000100.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1018284"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..649)
                     /locus_tag="ENO07_01850"
     CDS             complement(<1..649)
                     /locus_tag="ENO07_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00738.1"
                     /translation="MFKLLRLGIILMSVLALTFSFACEGDQGPQGPEGEPGEPGEPGE
                     DLTIAPPPDMYVSVAVFNQFDGTVLNYRSQDFVRITFDTSQTPSGDLIVGAFVDQSPI
                     IDGTDGDIDEWGVGEDLFESDIPLTGTIGDDNGIVEVSMRCVYDARYVYFFLRWTEET
                     IGDFTQSENRKLEEWINQGVGTFDPLIGGSEDRVWLMFLTDPNYIPSGADCLLGCE"
     gene            complement(864..>1530)
                     /locus_tag="ENO07_01855"
     CDS             complement(864..>1530)
                     /locus_tag="ENO07_01855"
                     /inference="COORDINATES: protein motif:HMM:PF13280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDS00739.1"
                     /translation="VNPLREISAAAFEVNQIGDAELLSAPLFPQLADKVLELIDRRIL
                     VGHNVLFDNGFLKNEFRLAGIEYPDPRMLDTRDIARGLISSPSYGLSSLVGQFEIEVE
                     NRHRAMSDCLATYQLFKHLLALGLEKNYSSLEQMAGRYMIEPEKLFESLPDWLQEALE
                     KGIEIEIEYMNRGNVTSRRKILPLSAFDSHGKLYIEAFCQSRKSKRTFLVERIKNVYP
                     DRK"
BASE COUNT          409 a          393 c          344 g          384 t
ORIGIN      
        1 cctcacaacc gagcaggcag tctgcgccgc tgggaatata attgggatcg gtcaaaaaca
       61 tgagccagac ccgatcctcg gaaccgccaa tgagtggatc gaatgtgccg accccctgat
      121 ttatccattc ttccagtttt cgattttcag attgggtgaa atccccgatg gtttcttcag
      181 tccatctcag gaagaagtaa acataacgag catcataaac gcatctcata gatacttcga
      241 cgattccatt atcatctcct attgtcccgg tcagcggaat atcgctttcg aacaggtctt
      301 caccgacgcc ccattcgtca atatcaccat cggtgccgtc gattatcgga gattgatcta
      361 caaaagctcc cacgatcagg tccccggatg gagtctgcga agtatcaaaa gtgattcgaa
      421 cgaaatcctg gctgcgataa ttgagcacgg ttccgtcaaa ctggttgaat acagctactg
      481 aaacatacat atcaggcggc ggggcaatag tgagatcctc gcccggctca ccgggttcac
      541 ccggctcacc ctcgggtccc tgcggtccct gatcgccttc acaagcaaag gaaaatgtca
      601 gcgccaaaac agacattaag attattccca ggcgcaacaa tttaaacatg tcttaacctc
      661 ccgtagagaa tcgtaaataa tttcgtggta attgattgta caagataatt ttcctcccaa
      721 tcaaagaatt gattatagaa caatctgacc caaaaagcaa gggaattttt gttttaccga
      781 cgattttgac cggccttcgt tcagtttcaa aaaattaaaa atatgagcag ctccatattc
      841 gacaataaac cgtgttaaaa tgcctacttg cggtcaggat agacgttttt gatccgctct
      901 accagaaatg ttcttttgga tttacggctc tggcaaaagg cctcaatata cagtttgccg
      961 tggctgtcaa aagccgaaag cggcaggatt ttgcggcggg atgtgacatt gccgcggttc
     1021 atatattcaa tttcgatttc tatccctttt tccagagcct cctgcagcca gtcagggagg
     1081 ctttcaaaca gcttctccgg ttcgatcata tatctcccgg ccatctgttc cagactgctg
     1141 taattcttct ccaatccgag tgccaggaga tgcttgaaga gctggtatgt ggcgaggcag
     1201 tcgctcatgg ccctgtgacg attttcgacc tcaatttcga actgcccgac cagggatgaa
     1261 aggccatatg agggcgaact tatcagaccc ctggcaatat cgcgagtatc cagcatacgg
     1321 ggatcggggt attcgatccc ggccaggcgg aactcattct ttagaaaacc attgtcaaat
     1381 aatacgttat ggcccaccag aatacgtctg tcgatcaact ccaaaacttt atcggccagt
     1441 tgcgggaaaa gcggcgccga tagaagctcg gcatccccaa tttggttgac ctcaaaagcg
     1501 gcggccgaga tctcgcgcag aggatttacc
//
LOCUS       DSEB01000101           10874 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000250, whole genome shotgun sequence.
ACCESSION   DSEB01000101 DSEB01000000
VERSION     DSEB01000101.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10874)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10874)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10874
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000250"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..417
                     /locus_tag="ENO07_01860"
     CDS             <1..417
                     /locus_tag="ENO07_01860"
                     /inference="COORDINATES: protein motif:HMM:PF02954.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00740.1"
                     /translation="PLRDRIEDIPFLVNKFIQQFAIEERCRPKIFDEGAYDVLYDHDW
                     PGNVRELKHLVEALLSLTESDIVMAEDVHAYLRRKPEREKKAQTLNEMVDDFKKETIR
                     KTLIKTGNNRSKAALLLGMDRANFHKLLKRLELEDL"
     gene            978..7223
                     /locus_tag="ENO07_01865"
     CDS             978..7223
                     /locus_tag="ENO07_01865"
                     /inference="COORDINATES: protein
                     motif:HMM:PF13229.4,HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HDS00741.1"
                     /translation="MRSGTYDSCATNLGGKMLKKKLFKLFLLAAVILLGGNLYAANID
                     VTQSTAITVNGEYDRNPSIVYDGSQYWLFYTKGDNTSTAGVRGPGYDPDADKYYVYYK
                     TAATIDGLAAASETLLNKPRPTNFDQRVVSATYYNGKIYAFVSSGQSGTDRSMYYYDF
                     NGTNWTGPTLLTSSSGGHINVTSDASYIYIVWESGDGSADFYSFDGSTLSAKVDISTD
                     NMPKINLMGSTLYVVSIEDATGDIEVYSAAAGASPSFSAHSTAITGAGLYDPCIFNDG
                     TNLYVITAPYVSGNDQQYLIQAYDAGATGSWSTSKMVTGGGYGGTYWWEYWPLGYYDG
                     TDLYVFFTTESASPAYSDGEIGMLKMDWSLDNDHFVHIQNAVNFATAGDQIDIDDGTY
                     YEALNLNKANLSLAGTGTSLVTIDCGGLPGFNSSGIYISADGVDLEGFTFIGEPATSA
                     PRYGIKFADVTGGSLTNLIIKEFYRTGVDMLGTDGVTITGCESKDNGGNGFQACDANN
                     ITYTNITTSGNAWGGVGIFTWGQYTPIGTDNIVFTGTNSFGESADDNGGLYLEEGNYS
                     DPNNPYPITFSTNIADGANITIQFSDFTHMLGGDSDNNNTYRRFYGSLTDVLSAAAGA
                     PGHITTGRYVKDLSDESFYVASFLTIQGAVDAADPGDLINVQAGTYTEQVYITKNDLT
                     IDGAGVDATIVKSPVTLTQYFVTGSNNNYPVMFIHGATGITLTEMTIDGDNQGDTNYR
                     FVGIGFWNGGGSVTTAKVINVMNSTFSGAQHGVGIYSSNDTGGPYTIVLNDVLVDDFQ
                     KNAIALLGSGLTVDLDNVTTTGEGPTSVTAQNGIQIGPGVTGTVYNCDISDIAYTGAS
                     WTATGFLNEGDITATGVDISGCQTGVYWIDGSGTFTGGTITAPLGDGFYAYNSTAASK
                     SGAPKLIPEPFDPQFSYAQNEDKAVMNVTLSNTDIVGTGATDSWGVGAFSYNATAPDE
                     VYLTVNNCKIDDWDYGVVAYDYGGPVFTTIFENAIINCTYALAASTTITNVQDGSGNY
                     FGTTDAATIAGMIDGAVFDYTPWLSTGTYTAPGFTGDFSTLYIDNDGAQAGTETRLIE
                     ALGLVTSSTIYLAPGTYPEGPQVVFDSDVTIVGDSKNTVTIVPTANTGSSGDSRGWFL
                     VNSGVTLDMSEVTMDGAGYNIYQAVRSYGDLILDDIIFQNMIFSTYLGFAVAIMDATP
                     TITYCDFSNIGRVGMILFGSGTTNGLVDNCTYVGKGDGDWLDYAVEFGAGAGGSVTNC
                     EIYNCTGVASSDGSTSAGIIATTYYGAGTSATLEGNYIHDNSTGFGIGYGTSDVTTAT
                     VTNNGFVGNEYGLTTSSSTGITLLAYGNTFNNDFNADDDAGGTWDNGSHGNCWSDYSG
                     TGVYTVGGDAGAVDNYPTVNCFIDMTPDDIVYHCTGSFDFDVEIGDGLVGLDAANFTI
                     QYPHYLDFVNATEASGNYQLFYNEFENATGYDSIQFNFGVLTGSQDGPASLFNVELTG
                     SPATCGIDDISMIYADLRGHDENDSIINLPSPLPDPISLVVDCSDPVLTVNTADGGYY
                     NVEPVINLEASDNCDLDAIYYQFDGCEGAGWSPIVSDLSGSTYGPLDWTLFASAWTGL
                     SEGAHCLYFKVMDDNGRGNSDSCSYSWCFTKDVTPPPPPANLTAEPGHNKVSLTWSNA
                     ASDFDHTVIVRNDYADGGGHGYPEYDDAYTEAAYPADTADGDLLYSGTASSHTDTYNL
                     TSTTRDVYHYAAFTVDLAGNTSAPVADAQDRSTSYWLGDVSPVLAYDGYVYFTDLSVF
                     SNSYGTSDGDVYYNNECDFGPTFNNSTKGIPTTDNQVEFEDLTIFAINFDDVSPSAKS
                     RPIFSDNPEVTETGIRVTSRLEGGSCFVEVFLENGTHEAKSLIAELTYDSEMMTYHST
                     AYNTQLVNTDRPVFTKTLEYENKVSVSAAVLGQGQVFAGSGTIATIRFDNVRSQGPEL
                     TLTRADIRNNDNKKLLGDFTAQTIMASAALPEAYELFQNRPNPFNPETEIAYSLPEET
                     HVTIKVYNIAGQVVSTLVDEVRPAGIHKVTWNGTNQSGSRVASGVYFYRMETSTFRKT
                     IKMILVK"
     gene            7280..8362
                     /locus_tag="ENO07_01870"
     CDS             7280..8362
                     /locus_tag="ENO07_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00742.1"
                     /translation="MRHPLKLLTILTVGLFCILYPAVLASQDGSGHVLISPEETTVTI
                     DDTFSIFLAIDTVELVKDFLVDIQVDTNIIRLDSCKRVSTFFTGPSGAFTYWKDTVQY
                     FGENDSAYVYEIQGSIFGPGNFVNGPGDLVRMKYTAVGYGISPVDFRYYEFRDTLVDP
                     QTHMGNIIELEGALDGQVIVCPTDQAIIYAGPNDIGFLSENGQTPDPQIFSVANPCFG
                     DLQWEASESCNWFELNSYSGTAPSDITVSIDISGVNPGLYLDSIAISSLDAANSPQYV
                     RVSLELTQSYLCGDANGDGSINVSDAVYIINYVFIGGAAPEPMASGEVNCDGNVNVSD
                     AVWIINYVFIGGNAPCDVDGDLEPDC"
     gene            8377..9087
                     /locus_tag="ENO07_01875"
     CDS             8377..9087
                     /locus_tag="ENO07_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00743.1"
                     /translation="MKNKIIVIIMLIIVSNCTVLFGQMPEMALMPQLSYTSGAGTFDI
                     KLQLLSNIYDLKAYSVAIQFDSTMIETHPDSIWEGPLLGSTGAPTFFWAGFSPDSGTL
                     YIDGAILGDGITTGSIGELATIRFNAENPVIPGECPLNFISVRARDATNSPLYYDTVN
                     ATVIICEYLLADANGDGTINVSDAVYIINFIFVGGPAPDPLLVGDANCDDTVNISDAV
                     YIINYIFVGGDPPCRVCY"
     gene            9636..>10874
                     /locus_tag="ENO07_01880"
     CDS             9636..>10874
                     /locus_tag="ENO07_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00744.1"
                     /translation="MKILPISIALFICILSCGSSGMNPAEAFNLEIVEEPSRLPYRFT
                     GTGCTNSWKFPISLDSSGIPALYIVQNNWRKPSDLSSIIFKWEDKERDNIIAQFNART
                     VFIISHYSGDFDHDYVEELAITYLIGDTVWLEILEPFEEKQHRILLEVGEDFDSSGSW
                     DGTVFAFNDFDMNADGFNEIFVSVSAGYDLSPRYLACVDWKKESIIWKYDMPPYISFD
                     HSYILRDPNSGEHVLLFGAGSFCNGVEGNGLDDCHSYLICVTLEGGMLWQKEMGGRYT
                     HTHPELIDYDNDGKPDIITEIYPEEDDRQTRDIIIVDIMGNTLDYIMMEKYLKNMQMF
                     DIDLDGQEELCLYFGDNSIECYNDQLELEVSFSCGAPLSFRDSYDFINQGKPQFICNV
                     RGIGLALFDSEFRQLAFLDEE"
BASE COUNT         2784 a         2697 c         2706 g         2687 t
ORIGIN      
        1 cccctgcgcg acaggatcga agatattcca tttctggtca ataaattcat ccagcagttt
       61 gcgattgagg aaagatgccg ccccaaaatc ttcgatgaag gggcctatga cgtcttatac
      121 gatcatgact ggcccggcaa tgttcgggag ctcaagcacc tggtggaagc cctcttgagc
      181 ctgacagaat ccgatatcgt aatggccgag gacgtccacg catatctcag gagaaaacct
      241 gaaagagaaa aaaaggctca aactcttaat gagatggtgg atgatttcaa aaaggaaact
      301 ataaggaaga ccttgataaa gacaggcaac aatcgttcta aggcagcgct gctgctcggc
      361 atggacaggg caaatttcca taaactgctc aagcgcctgg aactggagga cctgtagcca
      421 actgacctca gtttttgtgg tatttcgagg tatatattac ctcatatcgc ccttcacgct
      481 gttacagcaa aaaaggcctt tataaatcaa caacttacag ggggaattta aggcgttgaa
      541 tggcacgtta tttgtaatgc tataatagta agcagaggaa actcttaact aaacaggacg
      601 tggctttgag tggtttagcc catagggctg ggcccggggg agtagaggta agaatcaggg
      661 gggaaattag gtgtttaaat ttcccccttg ccctatttca aaagtactct tggagcctta
      721 gagaacttgt actcgcagaa agctcaacta taatccagcc gggaggggaa agctgattta
      781 gcataagaaa tttatatgca gtaagaattc atgtaaaatc gagcaaggag gtgcgatcga
      841 taaacaggtt tacaagccat taataataca tgcccgctca acatatccgg ggcttatcat
      901 ctttctttta actgaaacaa ccaagagtac cgctgctatc gcacccgcat tagctcgatc
      961 caacaggtac ttatactatg cgctcaggaa cttatgattc atgcgctaca aatttagggg
     1021 gaaaaatgct gaaaaagaaa ctatttaaac tcttcttact ggctgctgtg attctgcttg
     1081 gagggaacct gtatgcggcc aacattgacg taacccagag caccgcaata acagttaatg
     1141 gtgaatacga ccgtaatccc tcgatcgtat atgacggttc tcaatactgg ctcttctata
     1201 caaaagggga taacacgagc actgcaggtg tacgcggccc gggatatgat ccggatgccg
     1261 acaaatatta cgtctattac aagactgcag ccacaatcga tggtctggcg gccgcctcgg
     1321 aaacattgct caacaagccg cggccgacca atttcgacca gagagttgta tccgccacct
     1381 actacaatgg caaaatttac gcctttgtat ccagcggcca atcgggcacc gaccgttcca
     1441 tgtactatta tgattttaat ggtaccaact ggaccggccc gaccctgctg acctccagca
     1501 gcggcggaca catcaatgtt accagcgacg ccagttacat ttacattgtc tgggaatcgg
     1561 gtgacggcag cgccgacttc tattccttcg acggttccac actcagcgcc aaagtggaca
     1621 tcagcacaga caacatgcca aagataaacc tcatgggcag cactctctat gtcgtcagca
     1681 tcgaggacgc aaccggcgac atagaagtgt attccgcggc agccggagct tcgccgtcat
     1741 tcagcgccca ttctactgca atcaccggcg ccggccttta tgatccctgc atcttcaatg
     1801 acggcacaaa tctttatgta ataaccgctc catatgtaag cggcaatgat cagcagtatc
     1861 tgatccaggc ttatgatgcc ggggccaccg gctcctggtc aaccagcaaa atggtaaccg
     1921 gcggagggta cggtggaact tactggtggg aatactggcc gttgggttat tacgacggta
     1981 ccgatctata tgtcttcttc accaccgagt ccgccagccc cgcatacagc gatggggaga
     2041 tcggcatgct gaagatggac tggagcctgg acaacgatca cttcgtgcat atacagaatg
     2101 cggtaaattt cgccactgcg ggtgaccaga tcgatattga tgatggaacc tattatgagg
     2161 ccctgaacct taacaaggcc aacctcagcc ttgcagggac cggcacctcg cttgtaacta
     2221 ttgattgcgg aggactcccg ggcttcaata gttccggtat ttatatttcc gcggatggtg
     2281 tcgatctgga gggcttcacg ttcatcggtg aaccggcaac ctccgctcca agatacggaa
     2341 ttaaatttgc agatgtcacc ggcggcagcc tgaccaacct gatcattaag gagttctaca
     2401 gaaccggcgt agacatgctt ggaacagacg gtgttaccat caccggttgc gaatccaagg
     2461 acaatggcgg caatggtttc caggcttgcg acgccaataa tattacctac acaaatatca
     2521 ctaccagcgg caacgcctgg ggaggagtgg gaatatttac ctggggccag tacaccccga
     2581 tcgggaccga taatatcgtc tttacaggaa ccaacagctt cggtgaatct gctgatgaca
     2641 acggggggct gtacctggaa gagggtaatt acagtgatcc caacaatccc tacccgataa
     2701 cattcagcac aaatattgct gacggcgcca atatcaccat ccagttcagt gatttcaccc
     2761 atatgctggg cggggactcg gacaataata atacttatcg caggttctac ggcagtctca
     2821 ccgatgtatt gagtgcagcg gcaggagcgc ccggccacat taccacgggc cgttatgtta
     2881 aggatctgag cgacgagagc ttctacgtcg ccagcttcct gacaatacag ggtgcggttg
     2941 atgccgctga tcccggtgac ctgatcaatg tccaggccgg tacttatacc gagcaggttt
     3001 atatcaccaa gaatgacctg accatagacg gcgccggagt ggatgccacg atagtcaagt
     3061 ctccggttac tttgacacaa tattttgtga ccggctcgaa taacaattat cctgtgatgt
     3121 tcatccacgg agcaaccggc attaccctga cggaaatgac tatagatggc gataaccagg
     3181 gcgataccaa ttatcgcttt gttggcatag gcttttggaa cggcggcggc tcagtaacca
     3241 ccgcaaaggt cataaatgtc atgaacagca cgttctccgg cgcccagcat ggagttggta
     3301 tttactcatc caatgatacc gggggaccat ataccatagt cttgaatgat gttctggtag
     3361 atgatttcca gaaaaacgca attgccctcc tgggcagcgg gctcacggtt gatcttgata
     3421 atgtcaccac cacgggtgaa ggaccaacct cggtaaccgc ccagaatgga attcagatcg
     3481 gtcccggagt aaccggcact gtatacaact gcgatatttc tgatattgcc tacactggag
     3541 ccagctggac ggccacaggc ttcctgaatg agggtgatat tactgccaca ggagtggata
     3601 tctcgggatg ccagaccggt gtctactgga ttgacggcag cgggacattc accgggggca
     3661 caatcaccgc ccctctcggt gacggcttct atgcttacaa cagcactgcc gcatccaaga
     3721 gcggggcgcc aaagcttatt ccggaaccat tcgatccgca gttcagctat gctcagaatg
     3781 aagataaagc ggttatgaat gtcaccctca gcaataccga catagtcggt acaggcgcca
     3841 cagacagctg gggcgtgggt gcattctcat acaatgctac cgctcctgat gaggtttatc
     3901 tgaccgtaaa caactgcaag attgacgact gggattacgg tgtggtcgct tatgactatg
     3961 gcgggccggt cttcactaca atttttgaaa atgctattat aaattgcacc tacgcactcg
     4021 ctgccagcac gactatcact aatgtgcagg acggctcggg caattatttc gggacgaccg
     4081 acgcagctac aatagccggg atgatagatg gcgctgtttt tgattatacc ccctggctct
     4141 ccacgggaac gtacacagct cccggattca ccggcgattt ctcgacgctg tacatcgata
     4201 acgacggcgc ccaggcggga accgaaacaa ggttgatcga agcgctgggc ctggtaactt
     4261 ccagcacaat ctatcttgct ccggggacct atcctgaagg accgcaggtt gtgtttgatt
     4321 cggatgttac catagtcggc gacagcaaga acactgtcac catcgtaccc accgccaata
     4381 ccggcagctc cggcgattcg cgcggatggt tcctggtgaa ctccggtgtg acgctggata
     4441 tgagcgaggt gactatggat ggcgccggat ataatatata tcaggccgtg cggtcatacg
     4501 gggatcttat cctggacgac atcattttcc agaatatgat tttttccaca tatctgggat
     4561 ttgccgttgc gatcatggat gcaaccccca cgattaccta ttgcgatttc agcaatatcg
     4621 ggcgtgtggg catgatcctc ttcggcagcg gtacgacaaa tggactggtc gacaactgca
     4681 cgtatgtcgg caaaggtgac ggcgactggc tcgattatgc tgtcgaattc ggcgcaggcg
     4741 ccggaggatc ggtaacaaac tgtgaaattt acaattgcac gggcgtggcg tcatctgatg
     4801 gttccacctc ggccgggatt atagccacga catactatgg agcgggaacc agtgccaccc
     4861 tggaaggaaa ctatattcac gataactcca caggcttcgg cattggctat ggtacttcag
     4921 acgtaaccac cgccacagtc accaataacg gtttcgtcgg caatgaatat ggcctgacta
     4981 cttcgtcaag tacagggatc acacttctgg cctacggcaa cacgttcaat aatgatttca
     5041 atgccgatga tgatgccggc gggacctggg ataacggttc gcatggcaac tgctggtcgg
     5101 attattccgg caccggtgtc tatactgtcg gcggcgatgc cggggcggtt gacaattatc
     5161 ccaccgtgaa ctgcttcata gatatgaccc cggatgatat cgtctatcac tgtaccggca
     5221 gtttcgactt tgatgttgaa attggtgacg gcctggttgg ccttgatgcg gccaatttca
     5281 caattcagta tccgcattat cttgattttg tgaacgccac cgaggcgtca ggaaattatc
     5341 agcttttcta taatgaattc gaaaatgcca ccggatatga cagtatccag ttcaacttcg
     5401 gtgtgctcac aggctcacag gatggaccgg cgagcctctt taatgtagag ctgaccggct
     5461 cgcctgcaac ctgcggcatt gacgacataa gcatgatcta tgccgatctg cgcggccatg
     5521 acgagaacga cagcatcata aatctgccca gccccttacc ggatcctatc tctctggtag
     5581 tggactgttc cgatcccgtg ttgacggtca atactgctga cggcggttac tacaatgtcg
     5641 aaccggttat caacctcgag gcctcagata attgcgatct tgatgcgatc tactaccagt
     5701 ttgatggctg cgagggcgcc ggatggagtc cgatcgtcag cgatctctcc ggttccacct
     5761 atggtcctct ggactggacc ctgtttgcgt cagcatggac cggattgagc gagggcgcgc
     5821 attgcctcta cttcaaggtg atggatgata atggccgcgg caattccgat tcatgctcat
     5881 attcatggtg ctttaccaag gatgtcacgc cgccgccgcc gcccgcaaac ctgacggcag
     5941 aacccggcca taacaaggtc agcttgacct ggagcaacgc tgccagcgat tttgatcaca
     6001 cagtcattgt tagaaatgac tatgctgacg ggggcgggca cggttatcct gaatatgacg
     6061 acgcctatac cgaggctgct tatcccgccg atactgctga cggggacctg ctttattccg
     6121 gaacagcttc ctcgcatacg gatacatata acctgacatc cacaacccgg gacgtctatc
     6181 actatgctgc ctttacagtc gacctggccg gtaatacctc agcacccgta gctgatgccc
     6241 aggatcgatc aacctcctac tggctgggtg atgtttctcc ggttctggct tacgatggtt
     6301 atgtttattt cacggatcta tcggtattct cgaattcata tgggacttcg gatggggatg
     6361 tttattacaa caacgaatgc gatttcgggc cgaccttcaa taacagcacg aagggcattc
     6421 cgacaaccga taaccaggtc gaatttgaag atctgacgat ctttgcaatc aatttcgacg
     6481 atgtatcgcc gagcgcaaaa tctcgtccga tcttctccga caatcctgaa gtgaccgaaa
     6541 ccggtatcag ggtgacctca cgattggagg gcggctcctg ctttgtcgag gtattcctgg
     6601 agaatggaac acatgaagcc aaatctctga tagccgaact gacctacgat tcggaaatga
     6661 tgacatatca ttcgaccgcg tacaatactc agcttgtcaa taccgacagg ccggtcttta
     6721 ccaagaccct ggagtacgag aataaagtct cggtttctgc cgcagtgttg gggcagggac
     6781 aggtatttgc cggttccggg acgatcgcga caattcgctt tgacaatgtc agaagccagg
     6841 gtcccgaact gaccttaacc cgggccgata tccgcaacaa tgacaacaag aagctcctgg
     6901 gcgacttcac cgcacagact ataatggctt cggcggcatt gccggaggcc tatgagctgt
     6961 tccagaatcg tcccaatcca ttcaatccag agacagaaat cgcctacagc ctgcccgaag
     7021 agactcatgt taccatcaag gtttacaata tcgcaggtca agttgtaagc acactggtgg
     7081 atgaggtcag gccggctgga attcacaagg ttacatggaa cggcaccaat cagagcggtt
     7141 ccagagtagc ctccggcgtc tacttctatc gtatggaaac cagtacattc cggaaaacca
     7201 taaaaatgat tctggtgaaa tagattcaaa catgatccgg aggggggatt atcccctccg
     7261 gattattgag gtacatatta tgagacatcc tttgaaattg cttactattt tgactgtcgg
     7321 gctgttctgc atactttatc ctgccgtact tgcgtcacag gacggctcgg gacacgtact
     7381 catttcgcct gaagaaacaa ccgttaccat cgacgacacc ttcagtatct ttctggcaat
     7441 tgatactgtt gagctggtaa aggattttct ggtcgatata caggtggata ccaatataat
     7501 cagactggac agctgtaagc gggtcagtac atttttcacc ggacccagcg gagcattcac
     7561 atactggaaa gataccgtac agtatttcgg agaaaatgat tcggcttatg tctacgaaat
     7621 ccagggttcg atcttcggcc cgggcaattt tgtcaatggc cccggtgatt tggtgcggat
     7681 gaaatacacc gccgtgggat acggcatcag cccggttgac ttccgttact atgagttcag
     7741 ggatacactg gttgatcccc aaacccatat gggaaatatt atcgaactcg agggagctct
     7801 ggacggacag gttatcgtct gtcccaccga ccaggcaatc atttatgccg gcccgaacga
     7861 tattggcttt ttgtctgaga acggacaaac tcccgatcct caaatatttt ctgtggccaa
     7921 tccatgcttc ggggatttgc agtgggaggc gtcggaaagc tgcaactggt ttgagctgaa
     7981 ttcttattcc ggaacagctc cctccgatat cacggttagt atcgatataa gcggagtcaa
     8041 tcccggtctc tacctcgact caattgcaat aagctccctt gatgcggcca actcgcctca
     8101 atatgtgagg gtgtctctgg aattgacgca gtcctatctt tgcggtgacg ccaacggtga
     8161 tgggagtatc aatgtctcgg atgcggttta catcataaat tacgttttca taggaggcgc
     8221 tgcgcccgaa ccgatggcct cgggcgaggt gaattgtgac ggaaatgtca atgtcagcga
     8281 tgctgtctgg ataataaatt atgtcttcat aggaggcaac gcgccctgtg atgtggatgg
     8341 agatttggag cctgactgtt agggaggaaa atctatatga aaaataaaat aattgtgatt
     8401 atcatgctga taatcgtctc aaattgcact gtactttttg gccaaatgcc ggaaatggcg
     8461 cttatgcctc agctgagcta tacttcggga gcaggcacat tcgatataaa actccagttg
     8521 ctttctaata tttacgacct caaagcatac tcggtggcga tacaattcga ttccacaatg
     8581 atcgaaaccc atccggacag catctgggaa gggccattgc tgggcagcac cggcgcgccc
     8641 accttcttct gggcaggatt ctcgcccgat tcgggcacgc tttacatcga tggcgctatt
     8701 ctcggagatg gcataaccac cggttctata ggagaattgg caactattcg gttcaatgca
     8761 gaaaatccgg tgatacccgg agagtgtccc ctgaatttta tctccgtgcg cgccagggat
     8821 gcgaccaaca gcccgctcta ttatgacact gtaaatgcca cagttataat atgcgagtat
     8881 cttctggctg atgccaacgg tgatggcaca atcaatgtct cagatgcggt gtacataatt
     8941 aatttcatct ttgtcggcgg gcccgctccc gatccccttc tggtggggga cgcaaattgc
     9001 gatgatactg taaatatcag cgacgcggtt tatataatta attacatctt tgtcggcggc
     9061 gatccgccct gccgcgtatg ttattgattc tcagtttctg ataaataacc aacggcttaa
     9121 accgggcaga attgaccgat tttgaatctc agtctgctgg tgggctgatg gacgatccat
     9181 attttgtagc ggcgtttgca caattatccg tttgaagcat gatgcgcttt tacaacaaat
     9241 aagggctgtt tctgaggtca tatttacccg cagaagcaga ctttcttttt gacacaatct
     9301 tatctgcatg tcccccaatt cattaataat taaatttgta gagcatttcc cggcacggct
     9361 attgcataag gatttattta gtggggttgg gtaggcaacc ctgatatttg gggggaagcc
     9421 ttctcgacta ggggcgagct cgtgaaggtt ccaattgagg ccggcaatct tataagttgc
     9481 cggccttttt gagatttccc gccagcccca tgaattcaag ctgttttcgg tataagtcta
     9541 atcccccgga tgtcgatacg cctcgggatc aacaaaataa ttgacattta tatccttttt
     9601 gatatattcc agcggcttta aaatagaagc gtgtgatgaa aattcttccg atctctatcg
     9661 ccctgtttat ctgcatcttg agctgcggtt catccgggat gaatcctgct gaagcgttca
     9721 atctggaaat cgttgaagaa ccatccaggc taccataccg ctttaccggc acgggatgca
     9781 caaattcctg gaaatttcct atcagcctgg acagcagcgg aatacccgct ctctacatcg
     9841 tccagaataa ctggcgaaag ccgagcgact tatcatccat catcttcaaa tgggaagaca
     9901 aggaacgcga caatatcata gcccagttca atgcccgaac ggtctttatc atctcccatt
     9961 attccggcga cttcgatcac gactatgtcg aggagctggc cataacctat cttatcggcg
    10021 atacggtctg gctggagatt ctggaaccct ttgaagagaa gcaacatcga attctgcttg
    10081 aggtcgggga agattttgac agcagtggca gctgggatgg caccgtattc gctttcaacg
    10141 atttcgatat gaatgctgac ggcttcaatg aaatcttcgt aagtgtttca gccggatatg
    10201 acctttctcc cagatacctg gcctgtgtcg actggaaaaa agaatccatt atctggaaat
    10261 atgacatgcc cccttatatc tcattcgatc attcctatat actccgggat ccgaattccg
    10321 gtgagcatgt cctgctcttc ggggcaggtt cgttttgcaa cggagtggag ggcaatggcc
    10381 ttgatgactg ccattcctat ttgatatgcg tgacccttga gggcgggatg ctgtggcaga
    10441 aggagatggg cggcagatat acacataccc atccggaact catcgattat gacaatgacg
    10501 gcaagcccga tatcatcacg gaaatctatc ccgaagaaga tgataggcag accagagata
    10561 tcattatagt tgatattatg ggcaacaccc tggattacat tatgatggaa aaatatctga
    10621 aaaatatgca aatgttcgat atcgacctgg acgggcaaga ggaactctgc ctgtactttg
    10681 gcgacaattc cattgaatgt tacaatgatc agcttgaact tgaagtgtcc tttagctgcg
    10741 gggctccact gagcttcagg gacagttatg acttcataaa tcagggcaaa ccgcaattca
    10801 tttgtaatgt aagaggaatc gggctggcgc ttttcgactc agaatttaga cagcttgcct
    10861 ttttggatga agag
//
LOCUS       DSEB01000102            2669 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1005331, whole genome shotgun sequence.
ACCESSION   DSEB01000102 DSEB01000000
VERSION     DSEB01000102.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1005331"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1215
                     /locus_tag="ENO07_01885"
     CDS             <1..1215
                     /locus_tag="ENO07_01885"
                     /inference="COORDINATES: protein motif:HMM:PF13505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDS00745.1"
                     /translation="LIEYDDSLKITHYIIELAGTYYISPETAWRPYLTAGAGVTFWRV
                     DDQDGNTVQVPDLNDQPFDYEDQQLTVMFGAGMEYFLSDHFSIGAATRYHLFTEVLSD
                     FTDERDIGGSDGLDNPVGFFEFGLTLTGYLSSCDDDDDDGVCNEDDRCPETPAGCIID
                     EETGCELDGDGDGVCDGLDMCSDTPAGCKVDAAGCPLDSDNDGVCDGLDRCDNTPEDC
                     EVDEYGCPLDADGDGVWDCRDNCPDTPEGCKVDESGCPLDGDADGVCDGLDQCPGTPE
                     GLEIDDVGCPVSYEIQKELVLVGVTFLVNQATIKPESKKELDKVVESLKALPHVKLEV
                     AGHTDISGSYDNNVELSQRRAEAVRDYFIEQGIDPERLIAKGYGPDKPKYDNSTLEGR
                     RKNRRVELIRLD"
     gene            complement(1697..2524)
                     /locus_tag="ENO07_01890"
     CDS             complement(1697..2524)
                     /locus_tag="ENO07_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00746.1"
                     /translation="MRKSLIFSLFLAIPVILILSCSDSTFQVQIGDMADIRYGNSVYI
                     SDAGLTITFDGEVFDSRCPMNAYCVWAGQAEVEFTIQKDGEEAVVSKAIVSPNPLLEK
                     PARYPGYEIRMLQLLPYPVAPVPTDNSSRIATIRVNESDGGNDPGIDPVHFSNLPPSD
                     LMVDAFELEDVGISGDILTLTVSYSGGCKDHDFALYMAPPAFMESYPVQANIYLEHQA
                     YNDMCEAYITEDLQFNIRPIAEKYFLEYGEYDDINLNVFKYFEDQPSEYIRITYSPE"
BASE COUNT          678 a          644 c          690 g          657 t
ORIGIN      
        1 ctgatcgagt atgatgacag cctgaagatc acgcattata ttatcgaact tgccgggacc
       61 tactatattt ctccggaaac agcctggagg ccatacctta ccgccggagc cggcgtaacc
      121 ttctggcgcg tcgatgatca ggatggcaac acggtccagg tgcccgatct caatgaccag
      181 ccgtttgact acgaggatca gcaattgacc gtgatgtttg gcgccggtat ggaatatttc
      241 ctcagcgacc atttctcaat cggcgcggct acccgctatc acctttttac tgaggtattg
      301 agcgatttta cagatgagcg cgatatcgga ggctcagacg gactggataa tcccgtcggc
      361 ttcttcgaat tcgggctgac cctcacagga tatctcagtt cctgtgacga tgatgacgat
      421 gatggtgtct gcaacgagga tgaccgctgt ccggaaactc ccgccgggtg cataatcgac
      481 gaagagaccg gatgtgagct cgatggcgat ggagatggcg tttgtgacgg cctggatatg
      541 tgttccgaca cgccggcggg ttgcaaggtt gatgcggccg gatgtcccct ggattccgat
      601 aacgatggtg tctgcgacgg tctcgaccgc tgcgacaata ccccggaaga ctgtgaagtc
      661 gatgagtatg gctgtcccct ggatgctgat ggggacgggg tctgggactg ccgcgacaac
      721 tgccccgata ctcccgaggg ctgcaaagtt gacgaaagcg gctgtccgct cgatggtgat
      781 gctgatggtg tatgtgacgg gctcgaccag tgtccgggta cgccggaagg gctggagatt
      841 gatgatgtcg gctgcccggt ctcatacgaa attcagaagg agctggttct ggtaggagtt
      901 accttcctgg taaaccaggc cacgattaaa cctgaatcaa agaaggaact ggacaaggtt
      961 gttgaatcgc ttaaggcgct gccgcacgtg aagctcgaag tcgccggaca caccgacatt
     1021 tccggttcgt acgataataa tgtcgagctc tctcagcgca gggccgaagc tgtccgcgat
     1081 tatttcatcg agcagggcat tgatcccgaa agattgattg ccaaaggtta tggtccggac
     1141 aagccgaaat atgacaactc tacattggag ggtcggcgta agaaccgcag agtagaattg
     1201 atcaggctcg attaagtctg catcgaggaa cttgagtttg caaaatggcc gccgcaagtc
     1261 aggcggccat ttttttgtga ttttcgcaaa tgtcttacaa tactgcttga caagcggggc
     1321 ccattatccc tatattatat ataaggatca actccttttt gcaccgtatc tgagttaact
     1381 ggttttttat tataagtagc ttttttgcac ctttacagaa tccgaaactg caggagatgc
     1441 gcaggctgac cttgcattgc aatcttgtct gattggttgc gggcaaacca aaaaggaagg
     1501 aggcatgaag ggaacttgag gagctaattt tcaaattcag gatgcatttt ctgaaattgt
     1561 aaccggaatg cagttaagct gaaaatttga aatcagtccg ccttcaaaat tcctgcaaac
     1621 gcgagcagat tgcgtggagc gggagaaaat ggggctacac gaaggcgggc tgtcttgttt
     1681 gcggacaacc ctgcaactat tccggtgaat aggtgatacg gatatactca gagggctgat
     1741 cttcgaagta tttgaataca ttcagattga tatcatcgta ctcaccatat tccaggaagt
     1801 atttctcggc aatcggtctg atgttgaact gcaaatcctc tgttatatag gcctcgcaca
     1861 tgtcgttata agcctggtgc tcgagataaa tattggcctg caccggatac gattccatga
     1921 aggccggagg cgccatatac agcgcgaaat catgatcctt gcacccgccg ctgtagctga
     1981 cagtgagagt cagaatatcg ccgctaatgc cgacatcctc gagctcgaat gcatccacca
     2041 tcaaatcgga cggaggcagg ttagagaaat gcaccggatc aatcccggga tcgtttccgc
     2101 catcagactc attcaccctg atagtcgcaa ttctggaact attgtcagtc ggaaccggag
     2161 caaccggata gggtaatagc tgcagcattc gtatctcata gcccgggtat ctggcaggtt
     2221 tttcgagcag gggatttgga ctgactatgg ccttcgagac gacagcctcc tcgccatcct
     2281 tctggatcgt gaactcaacc tcggcctgcc cggcccatac acaataggca ttcattgggc
     2341 agcgtgaatc gaacacctca ccatcgaacg tgatggtcaa cccggcatcg gagatgtaaa
     2401 ccgaattgcc gtaccggata tctgccatat ccccgatctg gacctgaaag gtcgaatcgg
     2461 agcaactgag tatcaaaatc accggaattg caagaaataa tgaaaagatt aagctcttcc
     2521 tcatatcaat tccccttctg atacagttta tcttcactta ttgaatccat agtattatac
     2581 gatttcatgc cctctatcgt ccagtcaaat ctgtgaattt taaaaatttt caccaatctc
     2641 aacatcgttt aaaatgcaca atatcaagc
//
LOCUS       DSEB01000103            2669 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1005330, whole genome shotgun sequence.
ACCESSION   DSEB01000103 DSEB01000000
VERSION     DSEB01000103.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1005330"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..485
                     /locus_tag="ENO07_01895"
     CDS             <1..485
                     /locus_tag="ENO07_01895"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382547.1"
                     /note="Catalyzes the reversible oxidation of malate to
                     oxaloacetate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDS00747.1"
                     /translation="STRFRTFVAMELNVSMSDIQAMVLGGHGDTMVPLPQYTTVGGIP
                     ITQLIPKDRIEEIAQRTRMGGGEIVKLLKTGSAYYAPAMASVQMVEAVLLDRKRVLPA
                     SAYATGQYGIEDLYIGLPIVLGANGVEKILEIDLGEEGLQSLQKSAATYKEHLKDLGY
                     "
     gene            506..1750
                     /gene="icd"
                     /locus_tag="ENO07_01900"
     CDS             506..1750
                     /gene="icd"
                     /locus_tag="ENO07_01900"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HDS00748.1"
                     /translation="MAYKMITPPENGEKITIVDGELKFPDNPIIPVIEGDGIGRDIMK
                     AARRVLDASVEKSYGGKKKIVWFDVFAGESANDKYGEWLPQDTIDAIRDYIVAIKGPL
                     TTPVGGGFRSLNVTLRQVLDLYACVRPVKFYKGTPSPVKRPEKMDIVIFRENTEDVYA
                     GIEYRGTTAEADKVREFLVNEMGANIRENSGIGIKPISAFGSKRLVVKGIQYAIDRGR
                     KSVTLVHKGNIMKYTEGAFRDWGYEVAKEKFGGVTITEDELWEKYDGKQPEGKIVVKD
                     RIADSMFQQILTRTDEYEVVCTSNLNGDYISDAAAAQVGGLGMAAGANIGDYIALFEA
                     THGTAPKYADKDVINPSSVILSGVMMLQYMGWEEAATMIENAIEKTIQNKTVTYDLER
                     QMQGATKVGTSQYATHIIDNMK"
     gene            1823..2005
                     /locus_tag="ENO07_01905"
     CDS             1823..2005
                     /locus_tag="ENO07_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00749.1"
                     /translation="MKLTNPTEPSGQPPDGFLFANSIRMYHIGIFAGQFLKAESPAFD
                     GELTFQPGSGVTEIRR"
     gene            2002..>2669
                     /locus_tag="ENO07_01910"
     CDS             2002..>2669
                     /locus_tag="ENO07_01910"
                     /inference="COORDINATES: protein motif:HMM:PF07690.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS00750.1"
                     /translation="MMQKQEQQHTQDYSDKWIYFGVAALAAFMGTLDSSIVNVALPTL
                     SKEFNADVDVVSWVALSYILTMAALLLVGGKLLDVWGERRQFVIGFTLFTIGSGLCAF
                     SVSIYMLIFSRAFQALGGAVLMSSNQGLIARGFPPHQRGRILGIVGTVVSVGLASGPV
                     LGGFLIEHLGWRSIFWINLPIGAIAIMYSMRNLANNPSATKEYKFDWRGSIFMIVGLG
                     AFFLG"
BASE COUNT          668 a          645 c          727 g          629 t
ORIGIN      
        1 attccacccg tttccgtaca ttcgtggcaa tggagcttaa tgtctccatg tctgatatcc
       61 aggctatggt tctgggaggt catggcgata cgatggttcc gcttccgcaa tataccacag
      121 tgggcggtat cccgatcaca cagcttatac ccaaggatag aatcgaagag atcgcgcagc
      181 gtacccgcat gggaggcgga gaaatcgtca agctgctcaa gaccggctca gcctactacg
      241 ccccggctat ggcctcggtg cagatggttg aagcagttct ccttgaccgc aagcgtgtat
      301 tgcccgcatc tgcctatgcc accggacagt atggtataga agacttatat atcggcctgc
      361 cgattgtcct gggagccaac ggcgtcgaga aaattctcga gatagacctg ggcgaagagg
      421 gattacagtc gcttcaaaaa tcagcagcca cctataaaga acatcttaaa gatctcggat
      481 actaacagct aaggagaatt gataaatggc ttataaaatg attactcctc ccgaaaatgg
      541 agaaaagatc accattgttg acggtgaact gaaattcccg gataatccga ttatacctgt
      601 gatcgaggga gatggtatcg gccgtgatat tatgaaagct gcccgacgcg ttctggatgc
      661 atctgtagag aaatcctacg gcggcaagaa gaaaatcgtc tggtttgatg tcttcgcggg
      721 agaatcagct aatgataaat acggtgaatg gcttccacag gataccatcg acgctatccg
      781 tgattatata gtggctatca aaggtccttt gaccactcct gtgggagggg gatttcgttc
      841 cctgaatgtg accctgcgcc aggtgctcga tctatatgcc tgtgtgcgtc cggtcaaatt
      901 ctacaagggc acaccgtcgc ctgtcaaacg tcccgaaaag atggacatcg tgatcttccg
      961 cgagaacacc gaagatgttt acgccggtat tgaatatcgc ggaacaacgg cggaggccga
     1021 taaggtgcgg gaattcctgg tcaatgagat gggcgccaat atccgtgaga actccggaat
     1081 cggcatcaag ccgatctctg ccttcggctc caagcgcctg gttgttaagg gaatacagta
     1141 tgccattgac cgcggacgca aatcggtcac actggtgcac aagggcaata tcatgaaata
     1201 taccgaaggc gccttccgtg actggggtta cgaagtggcc aaagagaaat tcggcggcgt
     1261 taccattacc gaggatgagc tctgggaaaa atacgatggt aaacagccgg aaggcaaaat
     1321 cgtcgtaaag gatcggatcg ccgactccat gttccagcag attttgaccc gtaccgatga
     1381 atatgaggtc gtctgcactt ccaacctgaa cggcgactat atctccgatg ccgctgccgc
     1441 gcaggtgggc ggtctgggta tggctgccgg cgccaatatc ggtgattata tcgcactttt
     1501 cgaggccacc catggaacag ccccgaaata tgccgataaa gatgtaataa atcccagttc
     1561 ggtgattctc tccggcgtta tgatgttgca atatatgggc tgggaagagg ccgcgaccat
     1621 gatcgagaat gcgattgaga agacaattca gaacaagacc gtaacctacg atctggagcg
     1681 ccagatgcaa ggtgcaacaa aagtgggaac ttctcagtat gcaacgcaca ttatagataa
     1741 tatgaaatag cagtcggtat agaaatatac cgatcgactc tcagctggca ggcgggttaa
     1801 ctgtcagcaa acagatggga taatgaaact caccaatccc actgagccgt ccgggcagcc
     1861 cccggacggc tttttgtttg ccaattcgat tcgtatgtac catattggca ttttcgcagg
     1921 tcaattcctg aaagctgaaa gtccggcttt tgacggggaa ttgacatttc agccaggctc
     1981 gggcgtgaca gaaatacgga gatgatgcag aaacaagagc aacaacatac gcaagactat
     2041 tcagataaat ggatatattt cggggtggcc gcgctggcgg cctttatggg tacgctcgat
     2101 tcctcgattg taaatgtcgc cctgcccacg ctctcgaaag aattcaacgc tgatgtggat
     2161 gtggtctcat gggtggcttt atcgtatatc ctgaccatgg cggctctgct cctggtaggc
     2221 ggcaagcttc tcgatgtatg gggtgagcgc agacagttcg tgatcggttt cacgcttttc
     2281 accatcggct ccggcctctg cgcattctca gtttcaatat acatgctgat cttctcacgt
     2341 gcttttcagg cgctgggagg ggcggtgctg atgagctcga atcagggact gatcgccagg
     2401 ggtttcccgc cccatcagcg cggccgtata ctgggcatag tcggcacggt tgtttcggta
     2461 gggctggcct cagggccggt gctgggaggc ttcctgatcg aacacctggg atggcgctcg
     2521 atcttctgga tcaaccttcc gatcggagct atcgctatta tgtattccat gaggaacctg
     2581 gcgaataatc cctctgctac aaaagagtac aaattcgatt ggcgcggatc gatctttatg
     2641 atagtgggcc tgggggcgtt ctttctggg
//
LOCUS       DSEB01000104            8869 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000390, whole genome shotgun sequence.
ACCESSION   DSEB01000104 DSEB01000000
VERSION     DSEB01000104.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8869)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8869)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8869
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000390"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="ENO07_01915"
     CDS             complement(<1..1163)
                     /locus_tag="ENO07_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00751.1"
                     /translation="MRKVSCLVMALILSICMGTALADWMPDDGHKMHFPQYPDLLGWN
                     VNATNPVILADDWMCSQSGPVTDVHFWGSWMGGITGAIISFNISIHADIPANPPVQPY
                     SMPGATLWEYEVPFAWVIAQELSTDLDEGWYDPASGVFNYPDHNVYFQYNIFLDQYID
                     PADLFVQEEGVIYWLNISATVADPAVTQWGWKSSQDHWNDDAVFAFWGNLEWVDLYEP
                     PDFIQSLDLAFVITGPDEDGACCYPDPTNGQLSLCIQTTQSDCVNNLLGVWEGPGTVC
                     QGMEACCLIDGSCLDADALCCINELGGTPQGPGSACGPPEACCFSDGSCADLDPLCCI
                     DQGGTPQGPGTMCTTPVACCLNDGSCIMVDPECCDDLGGYLSPFSAVCLGDNNG"
     gene            complement(1392..2948)
                     /locus_tag="ENO07_01920"
     CDS             complement(1392..2948)
                     /locus_tag="ENO07_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HDS00752.1"
                     /translation="MKSRAVFSALAAAFILTINYNISAAQEIKAVYGRQVSFYRGELI
                     WFVEGINETELGYDDGSYDQYQHVTEEHISPAAAVKLDSLHPPLYVKSAKIFIVNVDH
                     FPNYPGDQYTPFFLSLHADSTGLPGSILGEQDLVGASGDWQSGGMWVKAEKNILLERD
                     SVLWVMMQWQPEFPTAPMFGLDKSIVRMNSFYSYVDLSGDRIWDMCSWGQYMMRAEAL
                     QNDIDGGVTLSPGAMMPDSFRVYSSGQPLVTPSDEYYDTTIVNSLHCRVKLPGPENYF
                     CLTSFAGGQESETSEIIMIEGSSTESADVRFEPDSMEIVSRSPNDTSLVLVMTNKNGG
                     EINYRISQIEIASEKQFSEVTVEYTPSEGRISDDLSDTILVSIATSPDAFGDYSISTE
                     FELWDSAQGYLKERYRIMLHIEELTGAEDDYSAEPDGFYLGQNYPNPFNNETIIPYKM
                     PRNTGEISVEIINLRGELVRKLVLRNLGNGFIRWDGCGKPGNKLPSGIYFLRVNAANF
                     RETRKMMLLK"
     gene            complement(2990..3598)
                     /gene="ruvA"
                     /locus_tag="ENO07_01925"
     CDS             complement(2990..3598)
                     /gene="ruvA"
                     /locus_tag="ENO07_01925"
                     /inference="COORDINATES: protein motif:HMM:PRK00116.2-4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDS00753.1"
                     /translation="MISYVKGKLAEKSPNHAVVENNGLGYYLIISANSYQALPDVGED
                     VALHTYLQIASQSDQMNLYGFSQKEEYDLFKTLISISGIGAKGAIKILSGISPRRFAE
                     VVAAEDVAFLTKLPGIGKKSAQRIIVELREKLPALTDKKQAVPGIDSDRAEEVIQALM
                     TLGMNAQESRRAIEKVIQTAGAKEAKILPTEEIIRRALTAGR"
     gene            complement(3595..4092)
                     /gene="ruvC"
                     /locus_tag="ENO07_01930"
     CDS             complement(3595..4092)
                     /gene="ruvC"
                     /locus_tag="ENO07_01930"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: protein motif:HMM:PRK00039.1-3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDS00754.1"
                     /translation="MPDEPVIMGVDAALSATGIGILKASGDDMEVIHTELIRTSGRQA
                     LPKRLEKLYNSVCELIERFNPDILALEDIFYSKNVKIALSLGQARGVILLACARNKLR
                     LQEFSAREIKQAVTGNGAASKEQVAYMVKSLLNMDGNFETYDISDSLAAALCMGYKIN
                     TPAVR"
     gene            complement(4085..4834)
                     /locus_tag="ENO07_01935"
     CDS             complement(4085..4834)
                     /locus_tag="ENO07_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDS00755.1"
                     /translation="MSGHSKWATIKRKKGKLDAQRGRTFTKLIKEISIAARQGGGDPD
                     NNPRLRSAISTAKAANMPQDNIKKAIQKGTGELPGVTYEEASYEGYGPGGVAVILEVV
                     TDNKNRTVAEIRHLFSKYNGNLGETGCVGWMFDKRGQIMVEKSKADEDKLMEIALDAG
                     ADDLVTHDDAYEVVTSFEAFERVKSALEEQDIEFMEAQVAMIPQTTVKVEGNKAESML
                     KLMEALEDHDDVQNVYANFDIDDSLMEKISA"
     gene            complement(5250..5954)
                     /locus_tag="ENO07_01940"
     CDS             complement(5250..5954)
                     /locus_tag="ENO07_01940"
                     /inference="COORDINATES: protein motif:HMM:PF13505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00756.1"
                     /translation="MKIVFIRGGKVKKALFLLLLACFWAISAASAQDLDLAGKFGFSA
                     FGSVDVPTGFTAADPMEGGLNRKLGFDFGVAADYFFTPLFGAGVDASYTFFDADDFEG
                     VESEDKFTVIMIGGHVKFAYPVVDLIVPYVSAGAGLAMPKIKDAASEFGPELVDIKIE
                     NQVYIAGNIGAMFFVTDMFSVFGEAGGVYIPLKHVETTVESDEGDLDGGDFPGNTPFL
                     NFKAGISLWLDLLPAD"
     gene            complement(6164..6832)
                     /locus_tag="ENO07_01945"
     CDS             complement(6164..6832)
                     /locus_tag="ENO07_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00757.1"
                     /translation="MKKIVVLLVTVLVCFAATAPAQVMDPTGMIDGSAFGIVDIPTGF
                     TQADWEEGGMARELGFGGGVSGEYFFHPFVGAGIHFMYHTFSAKDMGDEPMDDKFNAI
                     MIGAHAKGVYPLEMGIIPYATAGGGVALTKWKDMPGEGDLVEFDIDPALYITGNIGVM
                     YFVSPTVSVFAEAGGNYIMLDGTKYTVGSDETEFDFPKNVPFASIKAGVSVWFSLTPQ
                     EYEE"
     gene            complement(7316..8005)
                     /locus_tag="ENO07_01950"
     CDS             complement(7316..8005)
                     /locus_tag="ENO07_01950"
                     /inference="COORDINATES: protein motif:HMM:PF13505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HDS00758.1"
                     /translation="MKKMLIFAAIFVFGAVSMVSAQDLQGMFNISPYVGIGIPMGDLA
                     DDDPENADGLYRSIGFKFGASAEYFFTPAIGAGLEFLYAIHGAKDFDGFEPGDKLHTM
                     NIGAHFKYMFMPESMLRPYILAGVGLTMNKLKDLEFEVEEGVFVTGDLDLDTKMYFVG
                     GFGVKYWVSEMISIFGEAGFDYLLLDGAGAEFDGEPVIDPDTGEEAELQTNYYFLDFK
                     AGINVWFGGTE"
     gene            complement(8254..>8869)
                     /gene="fusA"
                     /locus_tag="ENO07_01955"
     CDS             complement(8254..>8869)
                     /gene="fusA"
                     /locus_tag="ENO07_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872259.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDS00759.1"
                     /translation="YKYKKQTGGKGQYGDVHIRVAPLPRGEGFEFVNAISGGVIPSKF
                     IPSVEKGIVEAMTEGSLAGYQVVDLKVELFYGSYHTVDSSDMAFKIAGSMAFKDAFLK
                     SNPVLLEPIYNLEVIVPDDYTGDIMGDISSRRGKILGMEPEGQGQRIKAQVPLAELYK
                     YSTAVRSMTQGRGVFSRSFSHYEEVPREIQDKLVEEAKKEKEQS"
BASE COUNT         2307 a         2456 c         1829 g         2277 t
ORIGIN      
        1 ccattattat ctcccagaca gaccgcgctg aagggtgata ggtagccgcc caggtcatcg
       61 cagcactccg gatcaaccat aatacagctg ccgtcattca aacaacacgc caccggggtc
      121 gtgcacatcg tacccggtcc ctgcggtgtt ccgccctgat cgatacagca caaaggatcg
      181 agatcagcgc agcttccatc cgagaaacag caggcttcag gcggtccaca tgcagaaccg
      241 ggaccctgcg gagtcccgcc aagctcattt atacagcaaa gggcatccgc atccagacag
      301 gaaccatcta tcaggcagca tgcttccata ccctgacaaa cagttcccgg cccctcccat
      361 acaccgagca ggttattgac acaatcgctt tgcgtagtct gtatgcataa tgacaattgg
      421 ccattggtgg gatcgggata acagcaggcg ccgtcctcat ccggacccgt aataacaaaa
      481 gcgaggtcaa gcgattgtat gaaatccggc ggctcataaa gatctaccca ttccaggttg
      541 ccccagaatg caaacacagc atcatcattc cagtggtcct gagaggactt ccagccccat
      601 tgggtcacgg ccggatctgc aactgtagct gagatattaa gccagtatat gactccttcc
      661 tcctgcacaa agaggtcggc cggatcaata tactgatcca ggaatatatt atactgaaaa
      721 taaacattgt gatccggata gttgaagacc ccggaggccg gatcatacca gccttcgtcc
      781 agatctgtcg aaagctcctg tgcgatcacc caggcaaaag gaacttcata ctcccacaga
      841 gtagccccgg gcatgctata gggttgaacc ggcgggtttg caggaatatc agcatggatg
      901 cttatgttaa agctgataat tgctcctgta atgcctccca tccatgatcc ccagaaatgc
      961 acatccgtaa ccggaccgga ctgtgaacac atccaatcat cggccaaaat caccggattc
     1021 gtagcattca cattccatcc caataaatcc ggatactgcg ggaaatgcat cttatggcca
     1081 tcatccggca tccaatccgc aagagcagtc cccatgcaga ttgagagaat gagggccatt
     1141 acgagacaag aaacttttcg cataacgcct cccattaaaa ataaatcaat atcgcgaaaa
     1201 acatcgagtt atatgtatat tttccatatc ttccaaggac caggcgaaag ggctcatttg
     1261 atggtccaat tgtaggggta ttatttctgc ttccgtaaca atgaatctta tattgactca
     1321 aatgtcaagt cttttttact tgaatttcta agggtagaat ttgggctttt taggacataa
     1381 atcgaatccc tttatttaag aagcatcatc ttccgagtct cccggaaatt agcagcattc
     1441 accctgagaa aatatatccc cgatggcaac ttattgcctg gtttgccaca accatcccac
     1501 ctgataaatc cattcccgag atttcttaag accagtttcc tcaccagttc tccccgcagg
     1561 ttgatgattt caaccgaaat ttctccggta tttctaggca ttttgtaagg tatgatcgtc
     1621 tcattattaa acggattggg atagttctga cccagataga agccgtcagg ttcggcagaa
     1681 taatcgtctt cggcgccggt caactcttca atatggagca tgatccgata cctttccttc
     1741 agataaccct gcgctgaatc ccacaattca aattcagtcg agattgaata atcaccaaac
     1801 gcgtctggcg atgtcgcgat agacaccagg attgtatccg aaagatcatc cgaaatcctg
     1861 ccctccgagg gggtatattc cactgtaact tcggagaact gcttctcgga agcaatctcg
     1921 atttgcgata ttctgtaatt tatctccccg ccgttcttgt tcgtcatcac caataccagg
     1981 ctcgtatcgt tgggcgatcg cgagacaatt tccatcgaat ccggctcgaa tcggacatca
     2041 gcactttccg tactgctgcc ttcgatcata ataatttctg aagtctccga ctcctggccg
     2101 cctgcaaatg atgtcaggca aaaatagttt tccgggccgg gcagcttgac ccggcaatgc
     2161 aggctattga cgatagttgt atcataatat tcgtccgagg gtgtcaccag gggctggccg
     2221 gaactgtaaa cccggaaact gtcaggcatc atcgcgcccg gtgagagcgt aacaccgccg
     2281 tcaatatcat tttgcagcgc ctcagctcgc atcatatatt gtccccagct gcacatatcc
     2341 cagattctgt cgcctgagag gtcaacatag ctgtaaaacg agttcatcct gacaatgctt
     2401 ttgtccaggc cgaacatcgg cgcagtggga aattccggct gccactgcat catcacccac
     2461 aagacactat cccgctccag caagatattc ttttcagcct tcacccacat tccccccgac
     2521 tgccagtctc ccgaggcccc caccaaatcc tgttccccca gtatgctccc gggcaatcct
     2581 gttgaatccg catgcagaga caagaaaaat ggggtgtact ggtctccggg atagttggga
     2641 aaatgatcga cattgacaat gaaaatcttc gcagatttca cataaagtgg cggatgcaga
     2701 gaatcgagct tgaccgctgc agcgggactg atatgctcct cggttacgtg ctggtactga
     2761 tcgtaactgc cgtcatcgta acccagctcg gtctcgttga tcccctcaac aaaccagata
     2821 agttcgcccc gataaaatga tacctgcctg ccatacacag ccttgatttc ctgggcagcc
     2881 gagatattgt agtttatggt taaaataaat gcggctgcca gagcgctaaa aacagctctg
     2941 gatttcatga gagacccctt ccttgcggcc gggcatgtga tgacgatgtc tatcttcctg
     3001 cagtcagcgc tctacgaata atctcctcgg tcgggagaat tttcgcctct tttgctccgg
     3061 ccgtctgaat aactttttcg attgcccttc tcgactcctg ggcgttcatc cccagggtca
     3121 tcagggcctg gataacctcc tcggccctgt ccgaatctat ccccggcacc gcctgcttct
     3181 tgtctgtcag tgccggaagt ttttctctta attcgactat aattctctgc gcgctcttct
     3241 tcccgatgcc gggcagtttg gtcaaaaagg ccacatcctc ggccgccacc acctcggcga
     3301 aacgtcgcgg ggaaatgccc gagagtatct tgatagcgcc cttggcgcct atgcccgaaa
     3361 tcgagatcag ggtcttaaag agatcgtatt cctctttctg cgaaaatcca tacaggttca
     3421 tctgatcgga ctgcgaggca atctgaagat aagtatgcag cgctacatct tcgccgacat
     3481 caggcagagc ctggtaactg ttggccgata ttatcaggta atatcccagg ccgttgtttt
     3541 ccaccaccgc atgattcggc gacttctcag ccagctttcc cttgacataa cttatcatcg
     3601 caccgccggt gtgttgattt tatatcccat gcataatgcc gcagccaggc tgtcggaaat
     3661 atcataggtc tcgaagtttc catccatatt cagcaggctt tttaccatat aggccacctg
     3721 ctccttcgag gccgcgccgt ttcctgtcac tgcctgcttt atttcccgcg cagagaattc
     3781 ctgcagccgc aatttattgc gcgcgcaggc cagcaggatc accccgcgtg cctgtcccaa
     3841 actcagggct atcttcacat tcttggaata aaaaatatcc tccagggcga ggatatccgg
     3901 attgaatctc tctatcaatt cgcaaaccga attatagagt ttttccagac gtttcggcag
     3961 ggcttgccgt ccggatgttc tgatcaattc ggtgtggatg acttccatgt cgtcgccgct
     4021 ggctttcaaa attccgattc ccgtggccga cagagcggcg tcaactccca taatcacggg
     4081 ttcatcaggc actaattttc tccatcaacg aatcatcgat atcaaaatta gcatagacat
     4141 tttgaacatc gtcatgatcc tcgagcgcct ccatcagctt gagcatcgat tccgccttgt
     4201 tcccctcaac tttgacggtg gtctgcggga tcattgctac ctgggcctcc ataaattcga
     4261 tatcctgttc ttccagggcc gacttgaccc tctcgaatgc ctcgaacgat gttaccacct
     4321 cgtaggcatc atcatgcgtg accaggtcat ccgcccccgc atccagggca atctccatca
     4381 acttgtcctc gtcggccttg gacttctcta ccataatctg cccgcgcttg tcaaacatcc
     4441 agcccacaca tccggtctca cccaggttgc cgttatattt tgagaagaga tgccgtatct
     4501 cggccaccgt acggtttttg ttgtcggtta caacctcgag gataactgcc accccgcccg
     4561 ggccgtaacc ttcataggag gcctcctcgt aggtcactcc gggaagttcg ccggtgccct
     4621 tctggatcgc cttcttgata ttatcctggg gcatatttgc ggccttggca gtgctgatgg
     4681 ctgaacgcag acggggattg ttgtccgggt cgcctccgcc ctgacgggcc gctattgata
     4741 tttctttgat cagcttggta aacgtccggc cccgctgggc atcgagttta ccttttttgc
     4801 gtttaatggt tgcccatttc gaatgacctg acatatctat tctccttaag tcctaacctg
     4861 atttaatata atttaagggc tcagccttaa aatgcaacag gaattatggg cttgtcaata
     4921 gttaaaacat ggtatacccg gcattgtctt caattccgga atgcagttcc aatctaatac
     4981 aaaaaggcct tcaggccaac agaaattcca aatacctcgc gccattccct gatatatcct
     5041 gtgtccagaa taaaagcgcg ttcataggag taatccgctg atccggctat ggcaatattt
     5101 tttgagatca gataggtata tcttagagaa cctttttaat ttgtccggca gggtcttaat
     5161 cccggtttat ggtgattttg agcctgcgca catggttgac aagcttagac tatgctacgt
     5221 ggatgccaca aatatcccgc ctgtaccact caatcagcag gaagcagatc gagccataaa
     5281 cttattccgg ccttgaaatt caggaatggt gtattgcccg gaaaatctcc cccgtccaga
     5341 tctccctcat cagactccac tgttgtttcg acatgtttga gaggaatata cacccctccc
     5401 gcttctccga acaccgaaaa catgtcggtg acaaagaaca tcgccccgat attgcctgca
     5461 atataaacct gattttctat cttgatatca accaactccg gaccaaactc cgaggcggca
     5521 tcctttattt tcggcattgc cagtcccgct cctgcggata catacggtac gatcaaatct
     5581 acaaccggat aagcgaactt cacatggccg ccgatcatta ttacagtaaa cttgtcttcg
     5641 gattcgacac cctcaaaatc atccgcgtca aaaaaagtat aagatgcgtc cacacccgcc
     5701 ccgaaaagag gggtgaagaa ataatcagct gccactccaa aatcaaaacc gagcttcctg
     5761 ttcagaccgc cctccatagg atcggccgca gtgaaccctg tgggaacatc gacacttcca
     5821 aatgcggaaa atccaaactt acctgccaaa tccagatcct gtgccgatgc ggcggatatg
     5881 gcccagaagc aggccagtaa aagaagaaac aatgcctttt tcactttacc tcctctaata
     5941 aatactattt tcagtcgtcc tctttaccca gaatactgtg ccagttcatc cctcagtctc
     6001 atatagctgt atgcagtcta aatccttgac ggaacagtgt caacaacaag tttcatatcc
     6061 tggaagaata cataaattga tattacggaa gtgtaaagcg agccataaaa aaagccccgc
     6121 ttgaaacggg gctttaagtc agaaacgatc tttcttattt tacttattcc tcatactcct
     6181 gcggagtcag gctgaaccag acactcacac cggcctttat gctggcaaaa ggcacattct
     6241 tgggaaagtc gaactccgtc tcgtcggagc ctaccgtgta cttggtgcca tccagcatga
     6301 tatagttgcc gcccgcctct gcgaagaccg aaaccgtcgg cgagacgaag tacataacac
     6361 cgatattccc cgttatatag agggccgggt cgatgtcaaa ttctaccagg tcgccttccc
     6421 cgggcatatc cttccacttc gtcagggcaa ctccgccgcc cgcggtcgca tacggaataa
     6481 ttcccatttc caggggataa acaccttttg catgggcgcc gatcatgatg gcattgaatt
     6541 tgtcatccat cggctcatcg cccatatctt tggcggaaaa agtatgatac atgaaatgta
     6601 ttccggcgcc aacaaaagga tggaagaaat attcgccgct cacgccgccg ccaaaaccaa
     6661 gctcacgtgc catgcctcct tcttcccaat cagcctgtgt aaatccggtc ggtatatcca
     6721 caataccaaa agcagaacca tcaatcatgc ctgtagggtc catcacctgc gccggagctg
     6781 tcgccgcaaa acaaaccagg accgtcacca gcaaaacaac tatctttttc acctttccct
     6841 cctttgaaag ggtttgatca ttatgttaat ctttttctat ctcccattac tgaaacagaa
     6901 cctccgccct tgagcctaca gttaatgtaa acccgatatg atattaaaca atatcataaa
     6961 tctgtcacac cggcttcgat ttaaactgcc tgctctaagt ttatgatacg tcaatttaag
     7021 ggatagttta agagactggc cgcaatctgt caataaaaaa gtcttaactc ggcaatataa
     7081 aaatcgatgt ttcgcccaaa taaatccctc tctgaattca agctgcccgg attagaagca
     7141 ccatcgtatt ttattgacag gtattactga aaaagattac tttcagcgac catcttaaat
     7201 caaatttaca ttttttccgg gaaagattag gcgttgccgc ctttctgtac tgatggaaca
     7261 agaaaaaaag ccccgcatat gcagggcttt ttatcaatct atatcttctc agatattatt
     7321 cagtgccgcc aaaccagacg ttgataccag ccttgaaatc aaggaagtaa taattggtct
     7381 gaagctcagc ctcttcacct gtatcaggat caatgacagg ttcgccatca aattcggcgc
     7441 ccgctccatc aagcaggaga taatcaaaac cagcttcacc aaagatagat atcatctccg
     7501 atacccagta tttcacaccg aaccccccga cgaagtacat cttagtatca agatcaaggt
     7561 ctcctgtaac aaatacgcct tcctcaactt caaactcaag atcttttaac ttattcattg
     7621 tcagacctac cccggcgaga atataagggc gaagcatact ttcgggcata aacatatact
     7681 taaaatgagc ccctatattc atggtatgca atttgtcacc cggctcgaag ccatcgaaat
     7741 ccttggcacc atgaatagca taaaggaatt caagccctgc gccaatcgcg ggggtgaaaa
     7801 aatactccgc tgaagcccca aatttgaaac cgatggaacg ataaagacca tcagcattct
     7861 ccggatcatc atcagccaga tcgcccatcg gaattccaat tcccacatac ggcgagatgt
     7921 tgaacatgcc ctgaagatcc tgtgcgctta ccatcgaaac tgcaccaaaa acgaaaatcg
     7981 ctgcgaagat taacatcttc ttcatttttg cctccttgct gtaaaagcaa aaccctctaa
     8041 ttagtttctt tacttttccg tgaaataatc aaacgaattg atactattgt agcagtatat
     8101 gcttccttca tcacctccct cttctataaa aaagcagatg gttaccgtta cacaatctaa
     8161 ctttttattt tacacagcct tatctgcggg aatctattcc cgcccttaaa cgatttcaac
     8221 aaaaactttt gattttttta ttacgaaaat cgattaagat tgttcctttt cctttttggc
     8281 ttcttccaca agcttgtcct ggatttcccg cggcacctct tcataatgcg agaaactcct
     8341 ggaaaatact ccccgcccct gggtcatcga acgcacagcg gtggagtatt tatataattc
     8401 ggccaatggc acctgggcct taatcctctg gccctgaccc tccggctcca tccccaggat
     8461 cttgcccctg cgggacgaaa tatcgcccat aatgtcgccg gtataatcat cgggcacaat
     8521 tacttcaagg ttgtatatcg gctccagcag aaccgggttg gacttcagga aagcatcctt
     8581 gaaagccatc gagccggcga tcttgaaagc catatcagag gaatcgaccg tatggtacga
     8641 tccatagaac agttccactt ttaagtccac aacctggtac ccggccaggc ttccctcagt
     8701 catcgcttct acaatacctt tttcgaccga ggggatgaac tttgatggta tcaccccgcc
     8761 gcttatggca tttacgaact caaagccttc tccgcgcggc aggggagcga cacgaatgtg
     8821 cacatcacca tactgcccct tgcctccagt ctgcttcttg tatttgtac
//
LOCUS       DSEB01000105            4060 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002106, whole genome shotgun sequence.
ACCESSION   DSEB01000105 DSEB01000000
VERSION     DSEB01000105.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002106"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..346)
                     /gene="eno"
                     /locus_tag="ENO07_01960"
     CDS             complement(<1..346)
                     /gene="eno"
                     /locus_tag="ENO07_01960"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009351620.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDS00760.1"
                     /translation="MSQILMIIGREILDSRGNPTVEVECYLDDGSFGRAAVPSGASTG
                     AHEALELRDGDKKRYNGKGVLKAVSNVNEKIGPALVKEGIPATEQKMIDEFLLELDGS
                     DGKKNLGANAILG"
     gene            complement(424..2592)
                     /locus_tag="ENO07_01965"
     CDS             complement(424..2592)
                     /locus_tag="ENO07_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00761.1"
                     /translation="MDKISFAFDSAWLLPAIAGLIIYLILAVLLYRKTNPPFSKKVRI
                     PLGIIRYAALFFVVFCLAEPVLSVDVKKEDEPVVVFLADNSTSIKTVENFEQKREFLE
                     DFVSGDFTSLLPPESNADFYLFSDTLHAAGNLDFEGRQTSLGNVINSIRENYKDKNLA
                     GIIVASDGLSNFGSDPIAASRQAGGPIYAVDLGPQRISKDIRIVDINHEPVAYADKPF
                     ELNIELEGRGFEKLSLPVTVSSEGRDLAHKNLEILGQGERQRFKVEVTPTEPGVKNFN
                     IKLPVQPDEELTDNNSRNLSIKVLKSKKRALLAGNKLNWEITFLNRVISSSPDFVVDL
                     AINAAQSRLPDKVFPANQDSLNQYDIIILVNCESQFISSNLNALNAYVTDNGGSLWFI
                     LGEKTRGRYPNQASQSVLPFNPRFEDRYYNDFAFHLQLTEEGKIHPVTRLVEDSRENT
                     LLWQSLPPMEEHLVLTQIKPNAQILAVHPEREYADRSVPLIFNHKFGRGKVLTFAAGP
                     IWKIGFLNAGYGEDDFAYRQIITNSVNWLTTEEDIERIRLASDQSIYKSGERVGLSAT
                     VLDENYSPLENSIVNVVVKPVGREDSLIVSMVQESPGRFSADLGLLPSGDYILDGRVV
                     WEDKVLKQVSGKFKVEGFSLEEETLFLPPDMMQKISQAAGGKRYTIADFENISEDLST
                     IPRFRTESYETRIAGNIWVLIIILALLSIEWMVRKRLQLL"
     gene            complement(2640..3620)
                     /locus_tag="ENO07_01970"
     CDS             complement(2640..3620)
                     /locus_tag="ENO07_01970"
                     /inference="COORDINATES: protein motif:HMM:PF00348.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDS00762.1"
                     /translation="MDRGDLKELLLPVESDLKKFDALMDEFLQANSELISGVVKHLLQ
                     KRGKRIRPAIAFLISRTAGLKHPRMVEAALAIELIHTATLLHDDVVDESDTRRGQETV
                     NYKWNNLVSVLMGDFFFAKAFRLLVETQSPELVRRVSLATEKVSFGELRQIEETFNWE
                     LDEDEYYNIIRDKTAALFSVSSSSCAILLNAGQKSIDQFGLFGERIGKAFQIADDILD
                     FVGDSSKTGKGIGLDLLQGKVTLPLIYSLRTGQKEETEKIMGTLNNGLNKRSLKTILR
                     FINDNGGIDFARKKAAYFGDGALEFICGFPKSRYRESLENLIKFTISRDN"
     gene            complement(3673..>4060)
                     /locus_tag="ENO07_01975"
     CDS             complement(3673..>4060)
                     /locus_tag="ENO07_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HDS00763.1"
                     /translation="LMRHPFPGPGLAVRILGEITQERLELVREADAIFIQSLRDFNYY
                     DKVWQAFCVLLPVQAVGVMGDERTYENVLALRAVSSTDGMTADWSRLPEDLLEHVSST
                     IVNKIKGINRVVYDISSKPPATIEWE"
BASE COUNT         1021 a         1066 c          954 g         1019 t
ORIGIN      
        1 cacccagtat ggcgttggcg cccagatttt tcttgccatc cgaaccatcc aattcaagca
       61 gaaattcatc gatcattttc tgttcggtgg caggaatgcc ttcttttacg agggctgggc
      121 cgattttctc gtttacatta ctaaccgctt tcaaaacacc cttaccattg tagcgtttct
      181 tatcgccatc gcgaagttcc agcgcctcat gggcgccggt cgatgcgccc gagggcacag
      241 ccgcgcgccc gaaggagccg tcgtccaggt aacattcaac ctcaacagtg ggattacccc
      301 tggaatcgag gatctcacga ccaataatca ttaaaatctg ggacatcatt ttctccttga
      361 ccttttaatt cgacaggaac ggaaattaag gatggacagg ccagaaatca acataaaaat
      421 caattacagc agctgcagcc ttttgcgtac catccattca atggataaga gggcaagtat
      481 tatgataaga acccagatat ttccggcaat cctggtctcg taggattcgg ttctgaatct
      541 ggggatagtg ctcaagtcct cggaaatatt ttcaaaatcg gcaattgtgt atctttttcc
      601 gcccgcagcc tggctgatct tctgcatcat atccggggga agaaagagcg tctcctcttc
      661 cagggaaaag ccctcaacct tgaatttgcc ggaaacctgc ttcaggactt tgtcctccca
      721 gaccacccgg ccatcaagga tgtaatctcc tgatggaagc aggcccagat cggcgctgaa
      781 acggccggga gattcctgga ccatggatac gatcaggcta tcttccctgc ccaccggctt
      841 gaccaccaca tttacaatac tgttttcgag cgggctgtaa ttctcgtcca gaacggtggc
      901 cgaaagaccg actcgttccc cgctcttgta gatcgactga tccgaggcca gccggatgcg
      961 ttcgatatcc tcttcagttg tcaaccagtt gacagaattg gtaataatct gcctgtaggc
     1021 gaaatcatcc tcgccatatc cggcgttaag gaagccgatc ttccagatcg gccccgccgc
     1081 aaatgtcagg actttgcccc gtccaaattt atgattgaaa atcaggggca ccgagcggtc
     1141 tgcatattct cgctcgggat gcaccgcgag aatctgcgca ttcggcttta tctgtgtcag
     1201 gaccagatgt tcctccatgg gcggaagcga ctgccagagc agggtattct ctctggaatc
     1261 ctccaccagg cgggtaaccg gatggatttt gccctcctcg gtcaactgta agtgaaaagc
     1321 gaaatcattg taatacctgt cctcaaaacg cggattaaac ggcagaaccg actgactggc
     1381 ttgattggga tatcttccgc gagtcttttc acccagaata aaccagagcg atccgccatt
     1441 atcggtaacg taagcattga gagcgttgag attggaagat ataaactggc tctcgcagtt
     1501 taccagtatt ataatatcgt attgattcaa tgaatcctgg tttgccggaa agaccttatc
     1561 cggcagccgg ctctgggccg cattgattgc cagatcgacc acgaaatcgg gagatgatga
     1621 aatgaccctg tttaaaaaag taatctccca gttgagcttg tttccggcca gcagagcacg
     1681 ttttttggac ttgaggactt ttatgctcaa gttgcggctg ttattgtcgg tgagttcctc
     1741 gtcaggttga accggcagct tgatattaaa attcttgacc cccggctcgg tcggggttac
     1801 ctcgaccttg aatcgctgac gctcaccctg gccaaggatt tccagattct tgtgggccaa
     1861 atcccggccc tcagaactga ccgtaaccgg aagactcagc ttttcaaatc cgcgtccctc
     1921 cagctcgata ttgagttcaa agggtttatc ggcatatgcg acaggctcgt gatttatgtc
     1981 aacaatgcgg atatccttgc tgatcctctg gggacccagg tcaacagcat aaatcggtcc
     2041 accggcctgg cgggaggcag ctatcggatc agaaccgaaa ttcgagaggc catccgaagc
     2101 cacaattatc cctgccaggt tcttatcttt gtagttctcc ctgatagaat tgatgacatt
     2161 gcccagcgag gtctgccggc cctcgaaatc gaggttgcca gcagcatgaa gtgtgtccga
     2221 aaagagataa aaatccgcat tgctctccgg gggcaggagg cttgtaaaat ctccggaaac
     2281 gaaatcctcc agaaattccc gtttttgctc gaaattctcc acggttttaa tgctggtgct
     2341 gttgtccgcc aggaaaacaa caaccggttc atcctccttc ttgacatcta cggaaagaac
     2401 cggttcagcc aggcagaata ccacaaagaa aagcgcagca taacggatta tgccgagcgg
     2461 aatacgcact ttctttgaaa aaggcggatt cgttttgcgg tacagcaaga ctgccaggat
     2521 gaggtagatt ataagtcccg caatagcggg caggagccag gcgctgtcga atgcaaaaga
     2581 tattttatcc aaaccaaaca ttattgtatt gtactgagat ttcagtggat agtttgcgac
     2641 taattgtcac gactgatagt aaactttata aggttttcaa gagattcacg ataacgggac
     2701 ttcggaaatc cgcagataaa ctccagcgca ccatctccga aatacgccgc ctttttgcgt
     2761 gcaaagtcga ttccgccgtt atcattaatg aatctcagaa tagtcttaag gcttctttta
     2821 ttgaggccgt tgttcagcgt ccccattatc ttctctgtct cttctttttg ccccgtacgc
     2881 agggagtata tcagaggcag ggtgactttg ccctgaagca gatcgagccc gatacccttg
     2941 ccggttttgg agctgtctcc cacaaaatcg agtatatcat cggcaatctg aaaggccttt
     3001 ccgatccgct ccccgaataa tccaaactga tctatactct tctgcccggc attcaaaagt
     3061 atcgcacatg aagaggaaga gaccgagaag agggcagctg tcttgtcccg aattatgttg
     3121 tagtattcgt cctcgtcgag ctcccagtta aaggtctcct cgatctggcg cagttcgccg
     3181 aaggacactt tctcggttgc aagtgacact ctccgaacaa gttccggcga ctgcgtttcc
     3241 accaggagac ggaaagcctt ggcaaagaag aagtcgccca ttaataccga aacaaggttg
     3301 ttccatttgt agttaacagt ctcctgaccc cggcgcgtat cggattcatc cacaacatca
     3361 tcatgaagaa gggtggccgt atgaatgagt tcgattgcca gtgcagcttc aaccatacgg
     3421 ggatgtttca gacctgcggt cctgctgatc agaaaagcaa tcgccggcct tatccttttg
     3481 cccctcttct ggagtaaatg cttgaccact ccggagataa gctccgaatt ggcctgcaaa
     3541 aactcatcca tcagagcgtc aaattttttc aggtcgctct cgaccggcag caaaagctct
     3601 tttaaatcgc ctctgtccat atcttagcta acgataatac caaaaatttg catgaagtca
     3661 aacaatttcg gtctattccc attcgattgt tgccgggggt ttggatgaga tatcatacac
     3721 cacccggttg attcccttga ttttattgac aatagttgaa gaaacatgtt ccagtaaatc
     3781 ttccgggaga cgcgaccagt cagcggtcat gccgtccgtc gaactgaccg ccctcaatgc
     3841 cagaacattc tcatatgtgc gctcatcccc catgaccccc acagcctgga ccggcagaag
     3901 cacgcagaag gcctgccaga ccttgtcata ataattgaaa tcccgcaggc tctggataaa
     3961 tatggcatcg gcctcgcgta caagctccag gcgttcctgc gtaatttctc ccagtatccg
     4021 aactgccagg ccggggccgg gaaaaggatg gcgcatcagg
//
LOCUS       DSEB01000106            2311 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1007350, whole genome shotgun sequence.
ACCESSION   DSEB01000106 DSEB01000000
VERSION     DSEB01000106.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1007350"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            202..1140
                     /locus_tag="ENO07_01980"
     CDS             202..1140
                     /locus_tag="ENO07_01980"
                     /inference="COORDINATES: protein motif:HMM:PF00491.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HDS00764.1"
                     /translation="MGIFEDTIRPDINLFYSRNDPNDIKMGEFVKSAPENYADADFVL
                     LGCPQDEGVRRNKGRVGAKDGPCAIRKALYKFSVPEKVGFCELFDLGDTNVEGSLEEI
                     HDRQHIIVKKILEDGKRLVILGGGNDISYPDCSALAEISNDLLVFNIDSHFDVREENI
                     RHSGTPYRQLLDEKQILPERLYEMAGKEIVNSPEYRRYLDETGAHVYPLEELRSKGMK
                     ETFRNILNENKSEHIFWGFDLDSVQAQDAPGVSAGYPIGLTAEEICYIAKIAGSDRRS
                     QILELTELNPKYDIHERTAKLASMMILYYLDASASI"
     gene            1141..>2311
                     /locus_tag="ENO07_01985"
     CDS             1141..>2311
                     /locus_tag="ENO07_01985"
                     /inference="COORDINATES: protein motif:HMM:PF01593.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDS00765.1"
                     /translation="MMSHEYDAVIIGSGPNGLAAAITMAMAELSVLVLEAKDTIGGGM
                     RSAELTEPGFIHDICSAVHPLAAASPFLRRLPLEKHGLQWIYPPIAAAHPLENADTAA
                     LKQSIQTTAKALGEDREAYIRIFEPLVNDWEKIASDILGPLRIPYYIPEALRFGLKAI
                     QSADSFVGRYFSNEKARALFAGMAAHSMLPLDKAITSAIALVLMIQGHSHGWPIPKGG
                     SQSIANAMESMILSLGGKIERNIKLESPGEIPSSKAVLFDLTPKQILKIYNDALPDRY
                     RRQLSKYRYGPGTFKIDWALSEAIPFKAEECREAGTLHVGGSFEEIAKSEEQVWHGIA
                     PEKPYIILVQPSLFDSTRAPEGRHTAWAYCHLPNGSETDMTDRIQDQIERFAPGFK"
BASE COUNT          624 a          553 c          581 g          553 t
ORIGIN      
        1 ccctcatacg gtcaccagcg gtgagggtgg cgtccacgac agcatttttg ccagcggcgt
       61 gattgaaccg aatgacacgt tcagcctcca gttccttgag gacggcattt ttccttacta
      121 cgatagttat cgtcctgata ctttgaatgg tgttgtggtt gttgaggaac ctccggtaca
      181 ataaatatat tatagaatct gatgggtatt ttcgaagata ctattaggcc ggatatcaac
      241 ctattttaca gccgcaatga ccccaacgat attaaaatgg gcgagtttgt gaaatccgca
      301 cctgagaact atgcggatgc ggattttgtc ttgctgggct gtccccagga tgagggtgtc
      361 aggcgcaaca agggcagagt tggggccaaa gacggcccct gtgcgattcg aaaagctcta
      421 tataagttct cggtacccga aaaagtcggc ttctgtgagc tattcgatct gggcgatact
      481 aatgtggagg gcagcctgga agagattcat gaccgtcagc acattattgt taaaaaaatc
      541 ctcgaggacg gcaaacgtct ggtcatcctc ggcggcggaa atgatatatc ttacccggat
      601 tgcagtgcgc tggcagaaat atcaaatgac ctcctggtct ttaatatcga tagtcatttc
      661 gatgtacggg aggaaaatat tcgtcacagc ggaacgccct accgccagct cctggatgag
      721 aagcagattc tgcccgagcg cctgtatgaa atggccggta aggagatagt caattctccg
      781 gaataccgcc gctaccttga tgaaaccggg gctcatgttt acccgctgga agaattgcgc
      841 agcaagggaa tgaaagagac gttcagaaac atcctgaatg aaaacaaatc cgagcatatc
      901 ttctggggct tcgacctgga ttcagtccag gcccaggatg cccccggggt gagcgccggt
      961 tatcctatcg gactgacagc tgaagagata tgctatattg ccaagattgc aggaagcgac
     1021 aggcgttcac aaattcttga gcttacagaa ttaaatccca agtatgatat tcatgagcgc
     1081 accgccaaac tcgcctcaat gatgatacta tattatcttg acgcatcagc ctcgatataa
     1141 atgatgtcgc acgaatatga tgctgtaata atcggatcgg ggcccaacgg ccttgctgca
     1201 gccataacca tggctatggc cgaactttct gtgctggtac tcgaggccaa agacacgatt
     1261 ggcgggggta tgcgttcagc ggaactgacc gaaccgggct ttatccatga tatctgttcc
     1321 gcggtacatc ccctggccgc agcctcgcca ttcctgcgcc gcctgccgct tgaaaagcat
     1381 ggactgcaat ggatttaccc gccaatcgcg gccgcacatc cgctcgaaaa cgcagataca
     1441 gcggcattga aacaaagcat tcaaactacc gctaaagcat taggcgaaga ccgcgaagct
     1501 tatataagga tttttgaacc attggtgaat gattgggaaa agatcgcttc tgatattctt
     1561 gggcctctcc gaataccgta ttacattccg gaagctcttc gattcggact taaggcaatt
     1621 caatcggcag acagctttgt cggaaggtat ttttcaaatg aaaaggccag agcgctattt
     1681 gcaggaatgg ccgcccattc gatgctgcct cttgataagg caatcacctc agccatagca
     1741 ctggtgctaa tgattcaggg gcattcgcat ggctggccga ttccgaaggg cggttcgcaa
     1801 tccatagcaa atgccatgga atcgatgata ttatctcttg gaggcaagat tgaaagaaat
     1861 attaagctcg aaagtcccgg cgaaatcccc tcctcaaaag cagtgctttt cgacctgact
     1921 cccaaacaga ttttgaaaat ctataatgat gcacttccgg atagataccg cagacagctt
     1981 tcaaaatatc gttatgggcc gggcacattc aaaattgact gggctttaag tgaagcgatt
     2041 ccgtttaaag cggaggaatg ccgtgaagcg gggactcttc atgtcggagg atcatttgaa
     2101 gagattgcaa aatccgaaga acaggtttgg catggcattg cgcccgaaaa gccatatatc
     2161 atcctggtcc agcccagcct ttttgattcc actcgagccc cggaaggaag acatacggcc
     2221 tgggcgtact gtcatctgcc caatggctcc gaaaccgata tgaccgatag aatacaggat
     2281 caaattgaaa gattcgcgcc cggatttaaa g
//
LOCUS       DSEB01000107            2053 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1009506, whole genome shotgun sequence.
ACCESSION   DSEB01000107 DSEB01000000
VERSION     DSEB01000107.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1009506"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..246
                     /locus_tag="ENO07_01990"
     CDS             <1..246
                     /locus_tag="ENO07_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDS00766.1"
                     /translation="VANLTRELKDNHIEIVVIAVPPQQAQAVCDDIVKADVKAILNFA
                     PINLKVPKGVSLRNENMAMELEHLSFCLVNDHYCEQG"
     gene            326..742
                     /locus_tag="ENO07_01995"
     CDS             326..742
                     /locus_tag="ENO07_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00767.1"
                     /translation="MPKNAAGCLLLVCLVLFMAGVSFSDPGDDCTIYGSLKINGQDAS
                     IGSRLEAYIDGEKIVSDETNQSGQYSLTIPKYDPANPDQKGYNSESDVVVIKVDGRDA
                     EPTVNPQPGSMKINLEVKTSLNVKLTTWGKIKALFK"
     gene            858..1196
                     /locus_tag="ENO07_02000"
     CDS             858..1196
                     /locus_tag="ENO07_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HDS00768.1"
                     /translation="MSISTHEKDGVVILEPEGKIMGGPEATELRNRLYELISTDNKKV
                     VIDLSHVEWMNSTGLGILISGMTSLRNNEGELKLANISEKVANLFEITKLDNVFEAYD
                     SADDAVGSFA"
     gene            1242..1664
                     /locus_tag="ENO07_02005"
     CDS             1242..1664
                     /locus_tag="ENO07_02005"
                     /inference="COORDINATES: protein motif:HMM:PF13581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDS00769.1"
                     /translation="MADKKSYRLEIPSSQNYLAEADRFLESHLKEHGIDDDSIADLAI
                     SATELINNAIIHGNKSDPHKIVTIELEFSDKHLSISITDQGQGFKPENIPSPISDDNI
                     MKEAGRGIFIVRSLVDDLQFEEPPEGGTRMIIIKKLAS"
     gene            1689..>2053
                     /locus_tag="ENO07_02010"
     CDS             1689..>2053
                     /locus_tag="ENO07_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00770.1"
                     /translation="MDYQLLIGVIYLIFSALLFFTGTVIFRENPYRRLNRVTSLLLIF
                     AGLAPLFAALGVFLGRTAGTVEGQVPFYENLFFLWELFFPQLVLFSLVFPTEHPILST
                     NTRVKYLIFIPHVFHILLVT"
BASE COUNT          582 a          461 c          471 g          539 t
ORIGIN      
        1 gtcgccaatc tgaccaggga gctaaaagac aaccatatcg aaattgtggt catagcggta
       61 cctcctcagc aggcgcaggc ggtctgtgat gatattgtca aagccgatgt caaggccata
      121 ttaaattttg ctcccatcaa tctaaaagtg cccaaggggg tctcgctgcg gaatgaaaat
      181 atggcaatgg agctggaaca tctgtctttc tgcctggtca acgatcatta ctgtgaacag
      241 ggctaatatc aacttcaagg cccaagtttt ttttcttgac aagattaatt caaatcttta
      301 tggtgcattt agtatgccct ctgtaatgcc aaaaaatgcg gcaggatgcc tgcttttagt
      361 atgcctggtt ttatttatgg ccggagtgtc attttccgat cccggcgatg attgcacgat
      421 ttacggcagt cttaaaataa atggtcagga tgcctcaatc ggcagcaggc tggaggcata
      481 tatcgacggc gagaagattg tctccgacga aaccaaccag agcgggcagt attctcttac
      541 aattcccaaa tatgacccgg ccaatcccga tcagaaggga tataattccg aatcagatgt
      601 tgtggttata aaagtcgatg gccgggatgc ggaaccgact gtaaatcccc aacccggctc
      661 gatgaaaatc aaccttgagg ttaaaacctc gctgaatgtc aaacttacca cctggggcaa
      721 aatcaaggcc ctcttcaagt aatttgcttt tcttgaaaaa tttgctacta aaaatccgtc
      781 taaactgtta tcataccgta ttaaaagata cattaaatcg atcttgtatt gtctattgat
      841 ttaactgagg aggcaagatg agcatctcca cccatgagaa agatggcgtg gtaattctcg
      901 aacccgaagg gaagataatg ggtggtcccg aggccacaga actcagaaac cggctatacg
      961 aattgatatc gaccgacaat aagaaagtgg tcatcgactt gagccatgtt gaatggatga
     1021 attcgaccgg acttggtata ctgatttccg gcatgacctc tctgcgtaat aatgaaggcg
     1081 aactgaaact ggccaatatc tcggaaaagg tcgccaatct attcgagatc acaaaactcg
     1141 ataatgtttt cgaggcttat gattcggcag atgatgcggt cggttcgttt gcataaataa
     1201 aaggacattc ccgggttgga taaagccgac tggtataaca tatggcagac aaaaaaagct
     1261 acagacttga aattccaagt tcccagaatt acctggccga agctgacaga tttctcgaaa
     1321 gccacctgaa agagcacggc atcgacgatg acagcattgc cgacctcgca ataagtgcaa
     1381 ccgagctcat taataacgca atcatccacg gcaataaatc agatccgcat aagatcgtta
     1441 caattgaact tgaattcagc gataaacatc tctccatttc gataacagat caggggcagg
     1501 gattcaagcc ggaaaatatt cccagtccga tttcggatga caatattatg aaagaggccg
     1561 gccgaggtat tttcattgtc agaagtcttg tggatgacct tcaattcgag gaacctccgg
     1621 aaggcggaac acggatgatt atcataaaaa aattagcttc gtaatcgata agaaggcgca
     1681 aggcgagtgt ggattatcag cttttaatcg gagtaatata tttaattttc tctgctcttc
     1741 tgttctttac cggcaccgtt attttcagag aaaatcccta tcgccgtctg aatcgggtaa
     1801 ccagcctgct gctcattttt gcaggccttg cccccctgtt tgcggcgctg ggcgttttcc
     1861 tgggcagaac cgcagggact gtggagggac aggtgccgtt ctacgagaac ctgttcttcc
     1921 tgtgggaatt attctttccg cagctggtgc tgttctcgct ggtatttccc accgagcatc
     1981 cgatcctgtc caccaataca agggtgaagt atcttatctt tatcccgcat gtgtttcata
     2041 tcctgctggt aac
//
LOCUS       DSEB01000108            9068 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000370, whole genome shotgun sequence.
ACCESSION   DSEB01000108 DSEB01000000
VERSION     DSEB01000108.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9068)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9068)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9068
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000370"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..951)
                     /locus_tag="ENO07_02015"
     CDS             complement(<1..951)
                     /locus_tag="ENO07_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13/DASS family transporter"
                     /protein_id="HDS00771.1"
                     /translation="MADTTAEKVEHLAKAGGIDWRRIFFIGLGAALFCIVYFSPPWAD
                     AVDPQEKHFVLSPQGKAALAIFLLAGIWWVFEVVPIGVTSITIGVLQALFLVRDPKEA
                     FGDFMDPSVWFILGSIVIGMSFAKTGLTRRMAYRMLLMVGERTSMIYLGAFVMTAALT
                     LIMAHTAVAAAVYPLLVAIYGLYAEDNRPTRFGKGLFMGMAYVAGAGSIITLLGAARG
                     AVAIGFYKRVIGEEITFFGLTYYMLPIGALMVIILWGFFMLVYKPEKKTIPGLRERVK
                     PLADRLGPITKTEILTLLIVFSSIAVLGLRSFIPALAPVNK"
     gene            1298..2743
                     /locus_tag="ENO07_02020"
     CDS             1298..2743
                     /locus_tag="ENO07_02020"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:PRK01406.2-4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDS00772.1"
                     /translation="MSEIRVRIAPSPTGYFHVGTARTAIFNYLYARAKGGKFILRIED
                     TDLERSSQEMVDVILDGLNWLNVRWDEGPYHQSRRYDLYPPFAEELLLSKRAYRCFCS
                     PEELAAKRDDAQKNKQTYQYDRSCRNLSDEEIDANLNQGKAFTVRLKLPIEGQASFHD
                     EVLGEMTKAYEDLDDFILVRSDGRPIYNFAVVVDDLLMKITHVIRGNDHVANTFKQNE
                     IYDALGKQRPVYAHLPLLLRPDRSKVSKRKGDKSVTEYRDDGILPDALFNYLALLGWS
                     PKDDREIMSQDEMIRVFDFKGINPTNAIFDPVKLSWMNSQYIMKTDNHSLVQLITPFI
                     IEADLATKYWIETRWQWMLRVVEVLKERCTTLRDFAENGSYFFKADFEYDPQGVQKRF
                     TAEGLADQLEQARKEMKKYYEIKKDIAEKIVREKAEEFGLKPAALIHPIRLALTGVTG
                     GPGLFDIVEILGQPEVDRRLSRAIEFIRNLD"
     gene            3012..3082
                     /locus_tag="ENO07_02025"
     tRNA            3012..3082
                     /locus_tag="ENO07_02025"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:3044..3046,aa:Gln,seq:ctg)
     gene            3340..3945
                     /locus_tag="ENO07_02030"
     CDS             3340..3945
                     /locus_tag="ENO07_02030"
                     /inference="COORDINATES: protein motif:HMM:PF00440.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDS00773.1"
                     /translation="MYQTFHSIQGIIVNKKLQIIAAAQPLFSQFGIKKTTTDDIAKRA
                     HISKATIYRYYRNKQEIFDDVVVSEVDHLQSAITEAVNAETTAVFKLRAYLLSKMGKL
                     RDLANLLQITREAWSDHWPYGTEVQDQILKQQKAIVADILEFGNRTGELRVKNVELTA
                     HLMVVSLQSVEYRWIFDTLNIPLSVYVDHMLKLIVNGIKKR"
     gene            3949..5514
                     /locus_tag="ENO07_02035"
     CDS             3949..5514
                     /locus_tag="ENO07_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDS00774.1"
                     /translation="MRKLSIGIIDLVAKAPTKNMWERVMGANFASIMPQVVATWCEEM
                     GHEVTLVTYTGREDLLEELPNNVDIVFIASFTEAALLAYALSNLFRSRGAVTAIGGSH
                     ARCYPQDAQKYFDYVLGFTDKKLIFEITSDCSRHRPFGMHLSAPQHPAELPSVRERWK
                     FIEQTTRKAPFIKIVPLLGSLGCPYTCDFCIDSTVRYQPMDPEAMKEDLRFLLGKFKR
                     PHVAWHDPNFGVRFDRCLDAIEEAVPPNRIDFIAESTLSLLSESRVKRLRRNGFKALL
                     PGIESWFELGKKSRTGKTIGMEKVEMVSEHINMILKYIPYVQTNHIVGLDGDSGPEPF
                     ELTKKFLNITPGAFPAYSLLSAFGQAAPLNLQFQENNRVLPFPFHVLSNSQMNIKPKN
                     YSWPEFYDRLIDLLKYSFSPRMVLRRFMANGETAPRILNIVRAISSEGSGRIKYFKEI
                     RRRLDSDKPLRRFFEQETNEIPQFLVQSIRKDLREFWDWLPDGALTHDPNAYLLSQQN
                     GCACCNVMGIKNP"
     gene            complement(5649..5939)
                     /locus_tag="ENO07_02040"
     CDS             complement(5649..5939)
                     /locus_tag="ENO07_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDS00775.1"
                     /translation="MNIYIGNLSFETTEEQLRQAFEDFGNVSAAQIIVDKYSGKSRGF
                     AFVEMSEKDEATAAISGLNGQELNGRELNVNEAKSRAQSSNHSSGNGSRRPY"
     gene            6353..7243
                     /locus_tag="ENO07_02045"
     CDS             6353..7243
                     /locus_tag="ENO07_02045"
                     /inference="COORDINATES: protein motif:HMM:PF00583.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDS00776.1"
                     /translation="MFSGYSTMKYTEYKNPDKFLAVCRPVFMEKEPLYGLMLGITLRL
                     IKHTHYYGGQPLLATIESETGLDLIALMTPPYKLQVASPNDYSLEAIRLLASSLHENG
                     WHVPAVMGERKVVKAFEANWNEIAGTRSHTGMRQRLYELRTVLPIKIPGGSLRKAQID
                     DLELSRRWGKSFYRDCFGDSEPVANIDNLVKNTIEEGNLFFWVNPEPVSMAALTRPTI
                     HGVSISYVYTPPHLRGNGYASAVVACLSRHALESGKKFCTLFTDLSNPTSNSIYQKIG
                     YDPVADIMEIQFTDAKSSAL"
     gene            complement(7272..8354)
                     /locus_tag="ENO07_02050"
     CDS             complement(7272..8354)
                     /locus_tag="ENO07_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDS00777.1"
                     /translation="MNNQKIYSVIAGTGSYIPPREVPNSAFLNAEFYDTDGSKFEKKN
                     EEIVGKLEEITGILERRYVTDDLVTSDIAYLAAKDALESSGTDKESLDYLVVAHNFGD
                     VRADNRRSDFVPSLAARVKHKLEIQNPRTVAYDLPFGCPGWLQGVIQADYFLKSGDAK
                     KALVIGAETLSRVSDPHDRDRMIYADGAGAAILEAKESDTPIGILSHATRSDTKDEAY
                     MLWMDESYNSSNGSQLFLKMNGHKLYLYAIKTVPLVVKESLDRIEMGIDDIQKVLIHQ
                     ANAKMDEAILKRIYKMYGNGSAPMYVMPMIISKLGNSSVATLPTLLDMMLKGKLNNHN
                     IKSGDNVVFASVGAGMNINAMVYRAP"
     gene            complement(8703..8972)
                     /locus_tag="ENO07_02055"
     CDS             complement(8703..8972)
                     /locus_tag="ENO07_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDS00778.1"
                     /translation="MQVSKLYVGNLSYSVTSEQLRELFANHGQVNDVRIIDGKGFGFV
                     EMSSVDEAEKAKDALDGSEYEGRTMKVDEARPKREDNNRRNNYRY"
BASE COUNT         2433 a         2213 c         2065 g         2357 t
ORIGIN      
        1 cttattaact ggcgccagag ccggtataaa tgatcgcaat cccagaacgg caatgctcga
       61 aaatactatt aaaagtgtca gtatctcagt tttggtaatc gggcccagtc tatcagcgag
      121 cggcttaact ctttcacgca atcccggtat ggtcttcttc tccggtttgt acaccagcat
      181 gaaaaagccc cagaggatta tgaccatgag cgccccgatc ggcagcatat aataggttaa
      241 tccgaaaaat gttatctcct caccaataac tcttttatag aacccgatgg ccacggcgcc
      301 gcgcgccgcg cccaggagtg tgattattga tcctgcgccc gcaacatagg ccatgcccat
      361 gaaaaggccc ttgccgaatc tggtgggccg gttatcctcc gcatacaggc cataaatggc
      421 aaccagtaat ggatataccg ccgcggccac ggccgtatgc gccataatca gcgtcagggc
      481 cgcggtcatc acaaaagccc ccaggtagat catgctggtc ctttcaccta ccatgagaag
      541 catacgatag gccatccgtc tcgtcagccc ggtcttggca aaggacatac cgattacgat
      601 ggaccccaga atgaaccata ccgagggatc catgaaatcg ccaaaagctt ccttgggatc
      661 ccgtaccagg aaaagggcct gcagcacgcc tatcgttatg ctggtgaccc cgatgggcac
      721 aacctcaaag acccaccata ttcctgccag tagaaatatg gccagggctg ccttgccctg
      781 gggggacagc acaaagtgct tttcctgcgg atcaaccgca tcagcccagg gaggcgaaaa
      841 atagacaatg caaaaaagcg cggcacccag gccgatgaaa aatatgcgtc tccaatcaat
      901 acccccggcc tttgcgagat gctcgacttt ttctgctgtg gtatcagcca taatgcgtat
      961 ctcctactct gttccagccg ccgttaatag gcccttttta gcactgtccg ggtataatga
     1021 caattgataa atttgccggt tatggctggc gcaagacctt ggtgaatggc cattgcgggg
     1081 tttcctcgaa gagaatttac ggtcattcat aatcattttg cggccaataa tttatcattt
     1141 atgccctccg atgtcaataa gcttaatacg gaatttgata aaaatttcac aaattgccat
     1201 ataatctcga ttctctgcaa gccgttcata atcccctaaa aaagtccttg ccaaaccggc
     1261 ctgcagggca tatcctctac gctgaaagga attggctatg agtgaaatac gagtacggat
     1321 cgccccctca ccaacgggct attttcatgt cggaactgcc cgcacggcaa ttttcaacta
     1381 cctttacgcc cgggccaaag gcggtaaatt tatattgaga atcgaagata ctgatctgga
     1441 gagatcatct caggagatgg ttgacgtcat cctcgacggc ctgaactggc tcaatgtccg
     1501 ctgggatgaa ggaccctatc atcaatcccg cagatacgat ctataccccc ctttcgctga
     1561 agaactgctg ctttcaaagc gagcgtacag gtgtttttgt tctccagaag agctcgccgc
     1621 aaaaagagat gacgcgcaga agaataagca gacttatcaa tatgaccgca gctgcaggaa
     1681 tctcagcgat gaggagatcg atgccaatct caaccaggga aaagcattca cggtcaggct
     1741 caagctgccc atagagggcc aggccagttt ccatgatgaa gttctgggtg aaatgaccaa
     1801 ggcctatgag gatctcgatg atttcatctt ggtcaggtcg gacgggaggc ctatttataa
     1861 ctttgctgtc gtagttgacg atctcttgat gaaaatcacc catgttattc gcggcaacga
     1921 tcatgtagcc aatactttta agcaaaatga gatatatgat gcactcggca aacaacgacc
     1981 ggtatatgcc catctacccc tgcttttgag acctgaccgc tcaaaagtct ccaagcgtaa
     2041 gggtgacaaa tcagtgactg aatatcggga tgatggtatt ctgccggatg cactcttcaa
     2101 ttatcttgct cttctgggat ggtctcccaa ggatgatcgg gagataatgt cgcaggatga
     2161 aatgatcagg gtttttgatt ttaagggtat caaccccacc aatgccattt ttgatccggt
     2221 caagctgagc tggatgaaca gccagtatat tatgaagacc gataaccata gcctggttca
     2281 gcttattacg ccatttatta tcgaggccga tctggcgaca aaatactgga tagaaacccg
     2341 ctggcagtgg atgctcaggg tggtggaggt tctgaaggag cgctgcacaa ctctgcggga
     2401 cttcgctgaa aacggcagtt attttttcaa ggctgatttc gaatatgacc cccagggagt
     2461 acagaagaga ttcaccgctg agggactggc agatcagctc gagcaggccc gtaaggaaat
     2521 gaaaaaatat tatgagatca agaaggatat tgccgagaaa attgtgcgcg aaaaggctga
     2581 ggaattcgga ttgaagccgg ccgccctgat ccatccaatc cggctggctt tgaccggtgt
     2641 gacaggcggg ccgggattgt ttgatatagt tgagatcctg ggtcagcccg aggttgacag
     2701 gcggctctca agggccattg aatttatccg aaacttagac taaggggagg cgatatggca
     2761 gaccagaaaa agatagagaa gctggaaaag gaaatcgaga atctggagat tcaatatgag
     2821 cagagccaga aagaggcttt gttcgataaa tccaggcagg ccaatatcta taagcagctc
     2881 aagaaaaagc ggaagcagct ggaacaactc cagaagaaat agatattata aaatgttgtc
     2941 aaaatcgtgc gcagataata agcttggtct tgacaagcaa tactgattta tatattttcc
     3001 ccaagtttaa ctgggcgatc gtccaatggt aggacatgcg gctctggacc gtaggatcgg
     3061 ggttcgaatc cctgtcgccc agtttttttc atgtttttaa tcgaaccacc aaattgaatc
     3121 tcataaaccg aaaaattcgg aattatatcg aagtcggagg ctggagacct caatattcca
     3181 gatttccagg attgtctccc cggaatctgc ttaatgtctt gcggctttgc atcttaggtt
     3241 ctcatctcaa tgccgtcaga gaatctgacc gaccgctttc aacgtctatt tgactccaat
     3301 tggaaataga cggaacaaaa aggaaacgaa aaacgtttaa tgtatcaaac gtttcattcc
     3361 attcaaggaa ttattgtaaa caaaaaattg cagattattg ctgctgctca acctttgttt
     3421 tcccagttcg gcatcaaaaa gacgactaca gacgacattg cgaaacgagc acacatatcg
     3481 aaagcgacga tctatcgcta ttaccgtaac aaacaagaga tcttcgacga tgtcgttgtc
     3541 agcgaggtgg atcacctgca gtcggccatc accgaagcag tgaatgccga aacgacagcg
     3601 gtttttaaac tgcgggccta tttgttgagc aagatgggca aactgcggga cctggctaat
     3661 ctgcttcaaa tcactcgtga ggcatggagc gaccactggc cctacggaac tgaagtccag
     3721 gatcagatcc tgaaacagca gaaggcaatc gtggcagata tccttgaatt cggtaacagg
     3781 accggcgaac tgcgggtaaa gaatgtggaa ctgacggcgc atttgatggt ggtatcattg
     3841 cagtcagttg aataccgctg gatttttgac actttgaata tccccctgtc ggtgtatgtt
     3901 gaccacatgc ttaaattaat cgttaacggc atcaagaaaa ggtaaattat gaggaaactc
     3961 tcaatcggga tcatcgatct cgtggccaaa gccccgacga aaaatatgtg ggaacgcgtg
     4021 atgggcgcaa actttgccag cataatgcca caggttgtgg caacctggtg cgaggaaatg
     4081 ggtcatgagg ttacgcttgt aacctacacc ggcagggaag accttctgga ggaattaccg
     4141 aataatgttg atattgtctt catcgcctca ttcaccgaag cggccctgct tgcctatgcc
     4201 ctgagcaatt tgttccgatc aaggggcgcc gtcactgcga ttggcggatc gcacgcacgc
     4261 tgttaccccc aggatgcgca aaaatacttt gattatgtgc ttggattcac tgataaaaaa
     4321 ctgatctttg agattacttc cgactgctcc cggcatcgcc cctttggtat gcatctatcg
     4381 gctccccagc atcctgcaga gcttccgagt gttagggaac gctggaaatt tattgagcaa
     4441 actacacgca aagcgccgtt tattaaaatt gtgcctcttc ttggcagcct gggctgcccg
     4501 tacacctgtg atttctgtat cgactcaacc gtgcgctatc agcctatgga tcccgaagca
     4561 atgaaggagg atctgcgttt cctgctgggc aaattcaaac gtccccatgt ggcctggcat
     4621 gatcccaact tcggcgtcag atttgaccgc tgcctggacg cgatcgagga ggccgtgccg
     4681 cccaaccgga tcgactttat cgccgagtcc accctttccc tgctttccga atcccgcgtg
     4741 aaacgtttga ggcgcaacgg cttcaaggcc ctgctaccgg gtatcgaatc atggttcgaa
     4801 ctgggcaaaa aatccagaac aggcaaaaca atcgggatgg aaaaagtgga gatggtctcc
     4861 gaacacatca acatgatact gaaatatatt ccctacgtgc agaccaatca tattgtaggg
     4921 cttgacggcg attccgggcc ggaaccgttc gaacttacaa agaaatttct gaacattacc
     4981 ccgggcgctt ttccggccta ttccctgctc tcggcattcg ggcaggccgc tcctctcaac
     5041 ctgcaattcc aagagaacaa tcgcgtcctt ccctttcctt ttcatgttct cagcaactcc
     5101 caaatgaata taaagcccaa gaactattca tggccggaat tctatgaccg tttgattgac
     5161 cttttaaaat actccttttc ccctcgaatg gttctgagac gatttatggc aaacggcgag
     5221 acagctccga ggatcttgaa catcgtacgg gcaatatcat ctgagggctc cggaaggata
     5281 aagtatttta aagaaattcg gaggcgcctg gacagcgata agcccctgcg aagatttttt
     5341 gagcaggaaa caaatgaaat accgcaattc cttgtacaat cgatacgcaa agaccttcgt
     5401 gaattctggg actggctgcc cgatggagcc ttgacccatg accccaatgc ctaccttctg
     5461 tcacagcaaa acgggtgtgc ctgctgcaat gtaatgggaa tcaaaaatcc atgatggcaa
     5521 gaatgatgtt cggggcccct gcgcatagat tcagaacaac agtgtcaaga aaaaaaagac
     5581 cggccagagg ccggtcttcg aacaaatcaa ttcacgtcag atttgatgtg caatttatta
     5641 gtttgccatt aataaggcct gcgagagcca ttaccactgc tgtggttgct gctctgggca
     5701 cgcgatttcg cttcattgac gttcaactcg cgtccattca actcctggcc atttaggccg
     5761 ctaatggcag cagttgcctc gtctttttca gacatttcca caaacgcaaa tcctcttgac
     5821 tttccactat acttgtcaac aatgatttga gcggctgaga cattgccaaa atcctcgaag
     5881 gcctggcgta attgttcttc tgtcgtttcg aacgacaaat tccctatgta gatattcatt
     5941 ctacactcct tggttgctca tcccgaaata aagtgccctc cagtgaagtc agatacttcg
     6001 tgacaaacgg tataatgcgc gtgattgcgc gttcaattcg aggttaagct ttagcggagt
     6061 tttgtagatt aagacaaggc tacggtaaag gtcgaactca aattaatgtg aacttttcat
     6121 tatacactat atctcgacaa aaacctacaa atacttgcgg aaattcaaaa ttatttttcg
     6181 acagccatca actaaattga taatcaagat catcaggccg caaggccgaa ccgcataaac
     6241 cagggcatct tctattcccc atcgcccggt aacgtcttgc cgatcatcac aattccactc
     6301 agatagttat ttatggttgt ccatttaaca ataatcctgc tatattacga caatgttttc
     6361 aggatattca accatgaaat ataccgaata caagaatccc gataaattcc tggctgtatg
     6421 ccgtcccgtc ttcatggaga aagaacctct ttacggtctg atgctcggaa taacacttcg
     6481 ccttatcaag catacccatt attacggggg tcagcccctg ctggcgacca tcgaaagtga
     6541 aaccggattg gatctgatag ccctgatgac cccgccctat aaacttcagg tcgcctcccc
     6601 gaatgattac tcattagaag caatccggct gctggcttcc agtttgcatg aaaacggatg
     6661 gcatgttcca gctgtgatgg gagagagaaa ggttgtaaaa gcgttcgagg ctaattggaa
     6721 tgagattgcc ggcacacgca gccatacagg aatgaggcag cgtctgtatg agctccggac
     6781 tgtcctgccg ataaaaatcc cgggtggcag cttgcggaaa gcgcaaatcg atgatctgga
     6841 gctgtcgcgc aggtggggca agtcctttta ccgcgactgc ttcggggatt cggaaccggt
     6901 tgcaaatatt gataacctcg taaagaacac gatagaagaa ggcaatctat tcttctgggt
     6961 aaatcctgag ccggtttcta tggcggcttt gacccgtcct accatacatg gtgtttccat
     7021 cagctatgtg tacaccccgc cgcatcttcg cggaaacggt tatgcctccg ccgtggttgc
     7081 ctgcctttcc cggcatgctc tggagagcgg caaaaagttc tgcaccctgt ttacggattt
     7141 aagtaaccct acatccaaca gcatatacca gaaaatcgga tatgatcctg tcgcggatat
     7201 tatggaaatt cagtttactg atgcgaaaag ttcagcctta taagattggc ttcttctgtt
     7261 tgagctgatg tctacggggc gcggtaaacc atggcattga tgttcatgcc ggcacccaca
     7321 gatgcaaaga cgacattgtc accacttttt atgttatgat tattcagctt gcccttcagc
     7381 atcatatcca gaagggtcgg cagagttgcc acggagctgt ttccaagctt ggagattatc
     7441 atcggcatga catacatcgg agcgctgcca ttgccataca tcttgtaaat tctcttcagg
     7501 atcgcctcat ccatcttggc attggcctga tgtataagta ccttttgtat atcgtcgata
     7561 cccatttcta ttctatctag gctttcctta actaccaacg gtacagtctt aatagcataa
     7621 agatataatt tgtggccgtt cattttcagg aaaagctgac tgccattgct ggaattatat
     7681 gactcatcca tccacagcat ataagcttca tctttggtgt cagacctggt ggcgtgggaa
     7741 agtattccta tcggggtgtc gctctccttt gcctcaagaa tagcggcccc tgcaccatca
     7801 gcatagatca tcctgtccct gtcatgggga tcggataccc ttgagagtgt ctcggcccca
     7861 ataaccagag cttttttggc gtccccggat ttcaggaaat agtcggcctg aatcacaccc
     7921 tgaagccatc ccggacagcc gaacggaaga tcataggcaa ccgttctggg attctggatt
     7981 tccaacttat gtttgaccct tgcagccaga gaaggcacaa aatctgaccg tctattgtcg
     8041 gccctgacat caccgaagtt atgggccacg accaggtaat ccaggctctc tttatcggtt
     8101 ccggatgatt ccagggcgtc tttggcggcc aggtaggcaa tatcggaggt caccaggtca
     8161 tcggtaacat aacgtctctc aagaatcccc gttatctcct ccagtttgcc gacaatttct
     8221 tcattctttt tctcgaattt cgatccatcg gtatcataaa actccgcgtt gagaaacgca
     8281 ctgttgggga cttctctggg agggatataa cttcctgtgc ctgcaattac cgaatatatc
     8341 ttctgattat tcataaatat ctctcgtcca ttagataatt ctgattgata tttataacct
     8401 gctgaagcct tctctcaaat agaagacaga actgtaaaac gagaaagctt aattattttt
     8461 gtttattaat acgtatttag tattgtttgt caaggaataa gatcaaggcc ataagataaa
     8521 ttcatggttt accctggacc ctgctatcaa gttttacaac ggccatctgc aagtatatat
     8581 ttttcaatcc ttttcacgct ttaatcaaaa gggaaagctt ttaagagctt tcccttcaat
     8641 aaataattta cctgaatcag ccagcattca tttttttact gttttaattc gttacaaata
     8701 ttttagtatc tgtaattgtt tctgcggttg ttgtcttccc ttttcgggcg cgcctcatca
     8761 accttcattg tgcggccttc atattcactt ccgtccagag catccttggc cttttcggct
     8821 tcatcgacgc tggacatttc cacgaatcca aagcccttgc catcgatgat tctgacatca
     8881 ttgacctggc catgattcgc gaataattct cttaactgct cagatgttac cgagtagctt
     8941 aagtttccta cataaagttt gcttacctgc atctttacct cgttgataaa atgtttctcg
     9001 tttgaatcat attattttga ggaagacgcg aagcaaagta gctgatctat cctgcataag
     9061 taatattc
//
LOCUS       DSEB01000109            8985 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000376, whole genome shotgun sequence.
ACCESSION   DSEB01000109 DSEB01000000
VERSION     DSEB01000109.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8985)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8985)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8985
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000376"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..881
                     /locus_tag="ENO07_02060"
     CDS             <1..881
                     /locus_tag="ENO07_02060"
                     /inference="COORDINATES: protein motif:HMM:PF04392.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00779.1"
                     /translation="SRAEKKHIAIVCSDSSLASIRSLKGIIQRLQTADIAVEYSELNL
                     KSEGPDNSIAKLKLLHPDLVISVGSPATILVKQNLPDLPLIFSTVLNPQTSGIIDAGD
                     SMPDITGASLDIPIDIQFQKFKMIYNDIRRIGVIYTEQTEPLIEEARRIAPELELELI
                     AIKIDSDRELPTALDSLCRIVDGIWTTADDLIYTPQATKFMILVSLRNKKPIMGFSPS
                     FVKSGALLGLNYDYKDIGRRAGGLAMRYLLGEPLSEIPIASPGVIYLHINLKTANQMG
                     IEVDQSLVDISKEIYK"
     gene            878..2566
                     /locus_tag="ENO07_02065"
     CDS             878..2566
                     /locus_tag="ENO07_02065"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00672.23,HMM:PF02518.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HDS00780.1"
                     /translation="MRRWFSIASLRFQAVAAISALIIITSAILVGFLIKSQKQNITAE
                     LEKRGASLVKMLANNSEYGVMIESDYILTDLMKTLSVERDFLFVAIQNPGGKIIAQVS
                     MLGPEFPLKSFLDEDVKNKMLSKTVLTEQKKIPHRDLELIEFVYPVFKYSVEGGISRE
                     ELGVVMPSLSDPDIQAEAIGLARLGISLEKVKKDIARMVWMTIFLTSLVVFAAIVVTI
                     TLVNFIIKPVENLVDVTERIAAGDLSHDVPVKSNDELGRLATSFNKMTDSLRNYREEV
                     EMYNRTLEQKIAERTSELEAAQKQLIQSEKMAAIGELAAGVAHELNNPMGGILGYSQY
                     ALEKLSSKKAADLEDNDIDMQCRFLEDIEQQARRCKTIIKNLLKFSRTSAKTEFEYFN
                     LNQALEETLTFVQHQLDMKNISLEKNLDQSLPSYYGNTSQLQQVFTNMMLNAQQAMPE
                     GGSLKIETRFSPPIGEFQGCVEVEFTDTGTGIPPEILNKIFEPFYTTKDVGKGTGLGL
                     SISYGIIKEHQGDILVKSKLGEGTTFTLVLPLENIVSDNRKTSASREEPAPQQK"
     gene            2681..3070
                     /locus_tag="ENO07_02070"
     CDS             2681..3070
                     /locus_tag="ENO07_02070"
                     /inference="COORDINATES: protein motif:HMM:PF00072.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDS00781.1"
                     /translation="MSGIKKILVVDDTPVIRRLLCDVLESEGYEIEEAIDGVEALEKI
                     RASKFDLVFCDIHMPRKNGFDTYIAAREIDPSLDFIMTDSFPDKLAEKTNEMGAICCL
                     AKPFELDELRKTLKQAEQLKKSKCPTK"
     gene            3055..4773
                     /locus_tag="ENO07_02075"
     CDS             3055..4773
                     /locus_tag="ENO07_02075"
                     /inference="COORDINATES: protein motif:HMM:PF00072.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HDS00782.1"
                     /translation="MPNKVDEKFAQSSRILIVDDEQIILNIASDVLKSEGYKIETTQD
                     PREALEKIKSEKYDFLLTDIKMPHIDGMTLAKEAQKISPDIGTIFMTGYASLDTAKEA
                     IKAGAYDYIMKPFELSELRKGIAKAVAKKAKLEESSGGHDLNRLSDLMEVLYNVGDRD
                     SLLKLSLGLALLNCNLEIGLIAAWDERSEKLEVAATSNVKDGDFEVISQLLNAEAAEE
                     CFNVSSPARNKGVKENPFFAKIIRQSAEVEKLSEFFKESYQTITIAVKSQKKFYIVLL
                     LQENGEDTLNERDLKLLSIVLSLISLAAENIALYEESKTALSELEKLHDDIVNLEKVA
                     AKGVMSAEIGHELNNFLNIVRSNFEMMTYRAKIKNLSEVAKYIDSINMSLDQMTKFTT
                     GLADAANLRTEKKMVNLNALLEDIAAFLSPQKRFRNIDIAKKLDNSLPNMMADSSQLS
                     QLFYNILNNASQALHEQNEGIIRVITRFDKENREAVIELSDNGPGFPPELAEKAFTSR
                     FTTKPGGHGFGLIVCSKVVNNHGGKIKVEKVEPHGTRFMITLPVISDPKAGEKADRQN
                     FAQIRV"
     gene            4855..6033
                     /locus_tag="ENO07_02080"
     CDS             4855..6033
                     /locus_tag="ENO07_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00783.1"
                     /translation="MKQPKDKRLVAVYTDIGRGHPNYLDSTLRAVEHRLDKLPKYIKI
                     TSVFEISRGMSLFGWKTIRRIYRSGAQGGLVSSLYNKARGPRSNGSRKNKAIRVLGKS
                     LHKYFEGYEGVVLVAHPILAHILGQACRVYYIHGEIASPLEFDFSRSEKIFVPLQETA
                     DAFISGGIPGDNIVVTGLMLEPELASKTDLIKAERLDRLEAGETATIGFYNSGAYPKS
                     HVWQIIEGAEHILKKNLGNIVISCGSEKRQFERFKSALSQYGPTTNAESFEKGESRVL
                     LQHHKDRQELTLQEIDLLPDIDLAVMAAHERTNWTLALQLPTIFLKPNIGNFAPMNYE
                     YACQHATVFPYQEGHITEALDKFIVAWREKDDILKKTDAEYDPGGCYQAADIIIRAID
                     "
     gene            6033..7745
                     /gene="ggt"
                     /locus_tag="ENO07_02085"
     CDS             6033..7745
                     /gene="ggt"
                     /locus_tag="ENO07_02085"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDS00784.1"
                     /translation="MQVRKIMSFKKIVLAILSIAILFLISCGERQNLRYFKGAIATVH
                     PLATETGMDILARGGNAIDCAVAVGFTLAVVYPQAGNIGGGGFAIIYINDSSYVTTLD
                     FREKAPIFAKQDMYLDTAGNIIENASLLGHMAVAVPGTVAGLLEMYRRFGTLPLEDLI
                     DPAIKLADSGFAVYEILEQELEASRDQLAQFETTRAIFFKNGEPLRKGERLVQTDLAE
                     TLKRIRKYGRSGFYGGRTAALFEASSLEAGGIITQTDLREYMPVWRAPIVFDFHNLRV
                     FSMGPPSSGGILLAQIFNMMEDFDLSSGDPYNPLFVHLFTAACKRAYADRAEYLGDID
                     FVDIPVQELISQGYARNRIADFDPTQATPSNMIDAGHAWRESESTTHFSIVDQYGNAV
                     GITYTINASFGAKVIVDSLGFFLNNEMDDFVAKPGEPNLYGLVGGEANKIEPQKRPLS
                     SMSPTLVFRGDSLLMVTGSPGGSKIITTVALSILRYFQFDMSLAHAVNQPKYHHQHLP
                     DILYYEIGALEKSEASKLEEMGYTLKEISAYGNLNVVARKSPEKRWEAESDRRRRGKA
                     ATVY"
     gene            complement(7749..8420)
                     /locus_tag="ENO07_02090"
     CDS             complement(7749..8420)
                     /locus_tag="ENO07_02090"
                     /inference="COORDINATES: protein motif:HMM:PF01066.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDS00785.1"
                     /translation="MSIGLRRIRGLIDIKFRHLFYVPNLLSILRFFFLIPIYHYLSKT
                     GTYADIMVLLCIAVAALTDIFDGYFARMLNQKTDLGKILDPLSDKIVIVVGMLGLVIH
                     RGFPLALVIFLGYRDLMILIGGLMIAKRSSEIIESNVWGKANTFMAATTAFVFVLSDG
                     WWGTYILMVITYLSILFSGTAYMFRGFEKLQVDTGMRFLWGIMFLIPVFLIFYFTPGH
                     LLGMS"
     gene            8580..>8985
                     /locus_tag="ENO07_02095"
     CDS             8580..>8985
                     /locus_tag="ENO07_02095"
                     /inference="COORDINATES: protein motif:HMM:PF13715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HDS00786.1"
                     /translation="MSFSRILLPVMIIFLVLGLLTTLNGEKLQPGIKGTITDKESGKP
                     IAHAVVELPGTEFKATTGSDGSYFIANVPEGSYLMTIRAHGYLRVNIVRVQVFSYATT
                     TVDYALTKSSNDNEKMESVFASELQKVEIEGVE"
BASE COUNT         2550 a         2181 c         2182 g         2072 t
ORIGIN      
        1 aatcccgggc tgaaaaaaaa catatcgcaa ttgtatgctc ggattccagt ctcgcatcca
       61 tcaggtcctt gaagggtata atccagcggc tgcaaacggc cgatattgcg gtagagtatt
      121 cggagctgaa cctgaaatct gaggggccgg ataattccat tgcaaaactg aaattgcttc
      181 accctgacct ggtcatatcg gtcggctcac cggccaccat attagttaag caaaaccttc
      241 ccgatctgcc cttgatattt tccacggtcc tgaatccgca gacctccggc ataatagatg
      301 ctggcgattc aatgcccgat atcaccggcg cctccctgga catccccata gatattcaat
      361 tccagaaatt caaaatgata tataatgata tcaggaggat cggtgtaatt tataccgagc
      421 aaaccgagcc attgatcgaa gaagccaggc gaattgcccc ggagctggaa ctggaactga
      481 ttgcaatcaa aatagactcc gaccgggaac tgcccaccgc actcgattcc ctgtgccgta
      541 ttgtggatgg tatctggacc accgccgatg acctgatcta tactccccag gcgacaaaat
      601 tcatgattct cgtttcgctc agaaacaaga agccgattat gggcttctct cccagcttcg
      661 taaagagcgg cgccttgctg ggactgaact atgattataa ggacatcgga aggcgggccg
      721 ggggcctggc catgcgttac cttctcgggg agccgttgtc ggaaatcccg attgcctcgc
      781 ccggggtcat atatctgcac ataaatctga agacggctaa tcaaatggga atcgaggtcg
      841 atcagtcgct ggtcgacata tcgaaggaga tctataaatg agacgatggt tcagtatagc
      901 atctctccgg ttccaggccg tggcggcaat ctcagccctg atcataataa catccgccat
      961 tctggtcggc tttttgatta agagccaaaa acagaatatc accgccgagc tggaaaaacg
     1021 aggcgcctcc ctggtcaaaa tgctggccaa taattcggaa tacggggtga tgatcgaatc
     1081 cgattacatc ctgaccgatc tgatgaaaac actttcggtg gaaagagatt tcctgtttgt
     1141 ggccatccag aatcccggag gaaaaatcat tgcccaggta tctatgctcg gaccagaatt
     1201 cccgctcaaa agtttcctcg atgaagatgt taagaataaa atgctgagca agaccgtgct
     1261 tacagagcag aaaaagatac cccaccgtga tctggaattg atagaatttg tctatcctgt
     1321 tttcaaatat tctgtggagg gcgggatatc cagggaagaa ctgggagtgg ttatgccttc
     1381 tctatcggat ccggatatac aggcagaggc gatcggcctg gcccggctgg gcatttccct
     1441 ggaaaaagtc aaaaaagaca tagcccgcat ggtctggatg accattttcc tcacatcgct
     1501 ggtagtattc gccgcgatcg tggtgacgat tacgctggta aactttatta tcaagccggt
     1561 cgagaacctg gtggacgtta cagagcgtat tgccgcgggc gatctttctc atgatgtgcc
     1621 ggtcaaatcg aatgatgagc tgggcaggct ggcgacatca tttaacaaga tgaccgactc
     1681 tctgcggaac taccgggaag aggttgagat gtacaaccgc acactagagc agaagatagc
     1741 cgagagaacc tccgagctgg aagcggccca gaaacagctc attcagtcgg agaagatggc
     1801 cgcaatcggt gaactggcgg cgggagttgc ccacgaatta aacaatccca tgggcggtat
     1861 cctgggttac agccagtatg ctctggaaaa actgtcttca aaaaaggccg ccgacctgga
     1921 ggataacgat atagatatgc agtgtcgttt cctggaggat atcgaacagc aggcccggcg
     1981 ctgcaaaacc attatcaaaa atctgctgaa attctcgcgc acatcggcca aaaccgaatt
     2041 cgaatatttc aatctgaatc aggccctgga agagactctg acattcgtgc agcatcagct
     2101 ggatatgaag aatatctcgc tggagaagaa tctggatcag agtctgccct cctattacgg
     2161 caataccagc cagttacagc aggtatttac aaatatgatg ctgaatgctc aacaggccat
     2221 gcccgagggc ggaagcctga agatcgagac gcgcttctct cctccaattg gcgaattcca
     2281 aggctgtgtt gaggttgaat tcaccgatac aggcaccgga attcccccgg agatcctgaa
     2341 caaaatcttc gagcctttct atacaaccaa ggatgtaggc aaaggtaccg ggctgggttt
     2401 gtccatatct tatggtatca ttaaggaaca ccagggggat atcctggtga agagcaagct
     2461 gggtgagggc actactttca ccctggtcct gcctctggaa aatatcgtaa gcgataacag
     2521 aaaaacttca gcaagccggg aagagcctgc ccctcagcaa aaataaattt ccccggccct
     2581 aaaatttatt aaactgccgc aaaataattc ccctaacttg ccgataagaa gtttagagat
     2641 tactggctat catcgttaag aattttaatg cagtaaaatt atgtctggaa ttaagaagat
     2701 actggtagta gacgacaccc ccgtaattcg cagacttctc tgtgatgtgc ttgaatccga
     2761 gggatacgag attgaagagg cgatagacgg cgttgaggcg ctggagaaga tccgggcctc
     2821 aaaattcgat ctggtttttt gtgatattca catgcccaga aagaacggct tcgatacata
     2881 tattgccgcc cgcgaaatcg atccgagcct ggacttcatc atgaccgaca gctttcccga
     2941 caagctggcc gaaaagacca acgaaatggg tgcgatatgc tgcctggcca aaccgtttga
     3001 gctggatgag ttaaggaaga ccctgaaaca ggctgaacaa ctaaaaaaat ccaaatgccc
     3061 aacaaagtag atgaaaaatt tgctcaatca tccagaatcc tgattgtcga tgacgagcag
     3121 ataatactga acatcgcatc tgatgtcctc aagagcgagg gctataagat agaaactacc
     3181 caggaccccc gtgaagccct ggaaaaaatc aaatctgaaa aatacgattt tctgctgacc
     3241 gatataaaaa tgccccacat agatggaatg accctggcca aggaggcgca gaagatctcc
     3301 cccgatatcg gcaccatctt catgaccgga tacgccagcc tggacacagc caaagaggcc
     3361 atcaaagcgg gggcctacga ctatattatg aaacccttcg agctctccga acttcgcaag
     3421 ggtatcgcca aagcggtagc gaaaaaggcc aaacttgaag aaagctccgg gggccatgac
     3481 ctcaaccgcc tttcagattt gatggaggtt ctctataatg tcggtgaccg ggacagcctg
     3541 cttaaactat cgctgggtct ggcgctctta aactgcaatc tggaaatcgg cctgattgcg
     3601 gcctgggacg aacgttcaga aaagctcgag gtggccgcta ccagcaatgt taaggacggt
     3661 gatttcgaag taatctcaca attgctcaat gcggaagcgg cagaagaatg cttcaatgtc
     3721 agctcacccg cccgaaataa gggagtaaag gaaaatccct ttttcgcaaa aatcataagg
     3781 cagtccgctg aagtcgagaa gctttcagaa tttttcaagg aatcctatca gaccattacg
     3841 attgccgtca aaagtcagaa aaaattctat atcgtcctgt tattgcagga aaacggcgag
     3901 gacacattaa acgagcgcga cctgaaactg ctctccatcg tgctttcgct gatctcgctt
     3961 gcggccgaaa atatcgcctt atacgaggaa tccaagacgg cgctctccga gctcgagaag
     4021 ctccatgacg acattgtcaa tctggaaaag gtcgccgcca agggtgtgat gtcggccgag
     4081 atcgggcatg agctcaacaa cttccttaat atcgtacgca gcaattttga gatgatgact
     4141 tacagggcca agattaaaaa cctctccgaa gtggccaagt acatcgacag cattaatatg
     4201 agcctggatc agatgaccaa gtttaccacc ggactggccg acgccgccaa cctgagaacc
     4261 gagaagaaaa tggtcaacct gaacgccctt ctggaggata ttgcggcctt cctttccccc
     4321 cagaagcgct tccgcaatat cgatattgca aaaaaactcg ataattcact tcctaatatg
     4381 atggccgaca gcagccagct atctcaatta ttttataata tacttaataa tgcctcccag
     4441 gcgctgcatg aacagaatga aggcataatc agagtcatca ccagatttga taaagagaac
     4501 cgcgaggcgg tgatagaatt atccgataac ggccccggtt ttcctcccga gctggccgaa
     4561 aaagcgttca cttctcgttt taccaccaag ccgggcgggc acggtttcgg tctgattgtc
     4621 tgttccaaag ttgtcaataa tcatggcggc aagatcaaag tcgaaaaggt cgaaccgcac
     4681 gggaccagat tcatgatcac cctgccggtt atctccgatc cgaaggccgg ggaaaaagcc
     4741 gaccggcaga atttcgccca gattcgcgtc tgagcatatc ctgatcagat gcacaaataa
     4801 ttacgctccg tttttattcg cattcatcgg cattctttat tatcttgaaa gtctatgaaa
     4861 caacccaaag ataaaaggtt ggtggctgtt tataccgata tagggcgggg gcatcccaac
     4921 tatctcgatt ccaccttaag ggcagtcgaa catcgtctcg ataagctccc gaaatatata
     4981 aagattacct cagttttcga aatttccagg gggatgtcgc tcttcggatg gaaaaccatc
     5041 cggcgtatct atcgttcggg agcgcagggc ggactggtct cttctttata taacaaagcg
     5101 cggggcccca ggtcaaacgg gagcaggaag aataaggcta tcagggtcct cggtaaatcc
     5161 ctgcataaat attttgaggg ctacgaaggt gtcgtcctgg ttgcgcatcc gatactggct
     5221 cacattctcg ggcaggcatg ccgggtatat tatatccacg gtgaaattgc ctctcccctt
     5281 gaattcgatt tctcccgatc agaaaaaatc tttgttccgc ttcaggaaac agcggatgct
     5341 tttatttccg ggggaattcc tggagataat atcgttgtca cgggcctcat gctggaaccg
     5401 gagctggcat ccaaaactga tcttataaag gcggaaagat tggaccggct ggaagcaggg
     5461 gaaacagcaa ctatcggctt ttataattcg ggcgcgtatc caaagtcaca tgtctggcaa
     5521 atcatcgaag gcgctgaaca tatactgaaa aaaaatctgg ggaatatagt aatttcatgc
     5581 ggcagtgaga agcggcagtt tgagcgtttc aagtccgccc tctcgcagta tgggccaacc
     5641 acaaacgcag agtcgttcga aaaaggcgaa agccgcgtgc ttctgcagca ccataaagac
     5701 agacaggaac ttacgctgca ggaaatcgat ctgctgcccg atatcgacct ggcggtaatg
     5761 gcggcccatg agcgcacaaa ctggacgctc gcgcttcaac ttccgactat attcctgaaa
     5821 ccaaatattg gtaatttcgc tccgatgaat tatgaatatg cctgccagca cgccacggtc
     5881 tttccttatc aggaggggca cattaccgag gccttggata aattcatagt tgcctggagg
     5941 gaaaaagatg atattttaaa aaaaacggac gcagaatatg atcctggcgg atgctatcag
     6001 gccgctgata tcattataag agccatcgat taatgcaggt gaggaaaatc atgtctttca
     6061 agaaaatagt tttggccatt ttatcaatcg caatcctttt cctgatttcc tgtggagaga
     6121 gacagaatct ccgctacttc aaaggggcta ttgccacagt gcatcccctg gcaaccgaga
     6181 caggtatgga tattctggcg cgcggcggaa atgccatcga ttgtgcggta gcggtaggat
     6241 tcaccctggc agttgtttat ccgcaggcgg gcaatatcgg cgggggcgga tttgcgatta
     6301 tatatatcaa cgacagttct tatgtcacca cgctcgattt ccgcgaaaaa gcgcctatat
     6361 tcgccaaaca ggatatgtac ctggataccg ccggaaatat aattgagaat gccagtttgc
     6421 tgggacacat ggcggtggcg gtgcccggta cggtggccgg actgcttgaa atgtatagac
     6481 gcttcggcac gcttccgctg gaggacctga ttgatcccgc cattaagctg gccgacagcg
     6541 gatttgccgt atatgaaatc ctggaacagg aactggaagc ttccagagat cagctggccc
     6601 aatttgaaac aaccagagcg atcttcttca aaaatggcga gcctctcaga aaaggcgaga
     6661 gactggtgca aaccgacttg gctgaaacat tgaaaagaat aagaaaatac ggacgctccg
     6721 gtttttacgg cggcaggacc gcggcgctat ttgaggcttc gtcactggag gcaggcggaa
     6781 ttatcaccca gaccgatctg cgggaataca tgccggtctg gcgcgcgccg attgtattcg
     6841 actttcataa tctgagagta ttctctatgg gtccgccctc atcaggagga attctgctgg
     6901 cccagatttt caatatgatg gaggatttcg acctgtcctc aggcgacccc tataatccgc
     6961 tgttcgtaca tctctttacc gcagcctgca aaagggctta tgcggatcgg gccgaatatc
     7021 tgggagacat cgatttcgtt gacattcccg ttcaggaact aatttcgcaa ggatatgcaa
     7081 ggaaccggat tgcagatttt gatcccactc aagctactcc ctcgaacatg atcgatgccg
     7141 gacatgcctg gcgagaatcg gaatcaacaa cacatttctc aattgtcgat caatacggta
     7201 acgcggtggg aattacatac acgatcaacg cctcattcgg ggccaaggtt atagtcgaca
     7261 gcctaggatt tttcctcaat aacgagatgg atgattttgt ggccaagccg ggggagccca
     7321 atctgtacgg cctggtgggc ggagaggcca ataaaatcga gccccagaag cgccccctct
     7381 catccatgag ccccaccctg gtctttagag gagattcact cctgatggta acgggctcac
     7441 cgggcggatc gaaaataatc accaccgttg cgctgtcgat cctgcgctac tttcaattcg
     7501 atatgtccct tgcccatgct gtcaaccagc ccaaatatca tcatcagcat ctgcccgata
     7561 tcctttatta cgaaatcggc gccctcgaaa aaagtgaagc ctcaaaactg gaggagatgg
     7621 gatatacatt gaaggagatc tcagcttatg gaaatctgaa tgtggtggca aggaaaagtc
     7681 ctgaaaaacg ctgggaggcc gaatcagata gaaggcgccg gggtaaggcg gctaccgtat
     7741 attagttctc aactcatgcc gagaagatgt cccggggtga aatagaatat tagaaatacc
     7801 ggaatcaaaa acatgatgcc ccataaaaac cgcatacctg tgtcaacctg aagcttttca
     7861 aagcccctga acatataggc tgtgcccgag aaaagtatcg atagatatgt aatgaccata
     7921 aggatatatg taccccacca tccatccgag agcacaaaaa caaatgctgt ggtagccgcc
     7981 ataaatgtat tcgccttgcc ccagacattg gattcaatta tctcactcga tctttttgca
     8041 atcatcaagc ccccgatcaa aatcatgagg tcgcggtagc ccagaaagat taccagggcc
     8101 agtggaaatc cgcggtggat tacaagcccc agcatcccga ccactataac tatcttatct
     8161 gacagggggt cgagaatttt ccccaagtct gttttctgat tcagcatccg cgcgaaatag
     8221 ccgtcgaaga tgtcggtcag ggccgccacg gcaatacaca gaagtaccat tatgtcagca
     8281 tatgtcccgg tttttgacag gtaatgataa ataggtatta gaaagaagaa tctcagaatg
     8341 gaaagcagat ttggaacata aaacagatgt ctgaatttta tatctatcaa acccctgatc
     8401 ctcctcaggc cgatcgaaat ctactaaata tctcaattta agtaatttaa gtcaatgaat
     8461 aaaaatccca ttaacgatcc tggttaattt tattaaacgt cgggaatatt gaggcgtcat
     8521 cattgtagag atcacagaag atttaaagac agcccataaa tcaaattaag gaggcagtta
     8581 tgtctttcag caggattctc ttacccgtaa tgatcatctt cctggtcctc gggcttctga
     8641 ccacactcaa cggagaaaag cttcagcccg ggatcaaggg aacgattacg gacaaagaaa
     8701 gcggcaagcc gatcgcacat gcggtcgtcg aattacccgg caccgaattc aaggctacca
     8761 ccggctctga cggcagctat tttatcgcta atgttccaga agggtcgtat ctcatgacca
     8821 tccgggcaca cgggtatctc agggtcaata tcgtccgggt tcaggttttc agctatgcga
     8881 ccacaacggt cgattacgcc ttaacaaaaa gctcaaacga taatgagaaa atggaatcgg
     8941 ttttcgcctc agaattgcag aaggtcgaaa tagaaggagt agaat
//
LOCUS       DSEB01000110            3912 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002280, whole genome shotgun sequence.
ACCESSION   DSEB01000110 DSEB01000000
VERSION     DSEB01000110.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002280"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..993
                     /locus_tag="ENO07_02100"
     CDS             <1..993
                     /locus_tag="ENO07_02100"
                     /inference="COORDINATES: protein motif:HMM:PF03901.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00787.1"
                     /translation="VLGGIAFMIRFQAGLALIAVPIAMVIQSRRWKQAIIFSLGIVVM
                     AALQGLIDIWSHGQFLGSISNYTAEYRNYLAGNPGPTPTIPGPWYRYILLILGIMVPP
                     FSLVFVGSIFGREVIRKHLILLLAFIIFVAVHSIIANKQERFIIPVFPVLLVLGSIGL
                     YYLYRNGGWYFRWKGLRAALWGWFALINTALLILFTFNYAHRGAIEPMIYLNRQEKVE
                     GVLFDTSERKKWLPYSYWNYRKPESVKLTPQYSLQEAINAGEVKPENPPKYIVVFSDG
                     RPDRYIEKYDTYLGDYEIVFHGKPSLMDFILNRLNPKYNHLNESWVLEFRTTQD"
     gene            complement(1073..1597)
                     /locus_tag="ENO07_02105"
     CDS             complement(1073..1597)
                     /locus_tag="ENO07_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00788.1"
                     /translation="MKKNKYIIGLVTACFIIIWASVVTAEGGKAGEDINWQVLSNGGT
                     EGSSTNYGLKGTVSQTAVDQGTSTNYKLRHGFWQIFATSGGPCQGLCGDANNDTNVNV
                     SDAVWIINFVFVGGDEPQPVLACGDANTDASVNVSDAVWVINYVFVGGGPPSTCSPGS
                     PNWYNGDCCPFGSS"
     gene            complement(1594..3447)
                     /locus_tag="ENO07_02110"
     CDS             complement(1594..3447)
                     /locus_tag="ENO07_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00789.1"
                     /translation="MRHIMLITIVSFLILASMPLSAEVPHSINYQGCLTDDAGDPVGD
                     GVYNIIFNIYGSAAGTDLLWSSGVKAVQVTDGLFTYMLGSSTPFPADLFVADTTRYLG
                     ITVGADPEIFPRTKLTSTAYAFHAAIADFLTLPADLETADDFNPALRIRNIGDGGAVY
                     GVCAGGYAGYFDGDGRFTGDLTIDGILNYPGLGDITAVNTPAGSGLSGGATSGDVTLG
                     VASGGITSDHILNNSIVDNDISASADIAPTKISGTAAVLNAGNTFTMGNYFNGESTMN
                     DAQIAYRRGGSHRWRIREEAGAGLEFFQVYDDGNILRNRTVMEIGDDNTVGIGEVLFN
                     RKLQITWDINSTGSWFGQATEVTNQDAGDLYGIYSSAQHPIAGVAGNCYAVYGYARSD
                     GASRLGVYAFSEAQNYNITTGETYGLRGTAYDGATAFGIYASGGSATTNWAGYFSGNC
                     RVTGTFDNSKSTMRIDHPTDPENRVLEHATINSPEMLNVYSGNVTTNANGEAIVQLPD
                     YFESLNKDFRYQLTVIGSFAQAIIKEKISNNQFLVATSEPDIEVSWQVTGVRHDAFAE
                     SHPVTVESEKTAEEKGYYINPEAFGFDVTRSTEYSTNAEFRREIDERRERE"
BASE COUNT          997 a          963 c          941 g         1011 t
ORIGIN      
        1 gtactgggtg gaatagcgtt tatgatccgt tttcaggctg ggctggcgct gatcgcagtt
       61 ccgattgcga tggttatcca gagcaggaga tggaaacagg caattatatt ctcgctggga
      121 atagtcgtga tggcggcgct tcagggtctg atcgatatct ggagccatgg acaatttctg
      181 ggaagtatca gtaattatac cgctgaatat cggaattacc tggccggcaa cccgggtccg
      241 acgccgacaa tcccggggcc atggtacagg tatatactgc tgatccttgg aattatggtc
      301 cctccgttct cactcgtttt cgtcggctcg atattcggcc gggaggtaat cagaaaacac
      361 ctgattttac ttcttgcatt tataattttt gtcgccgtgc actcgattat agcgaacaag
      421 caggaaagat tcatcattcc ggtcttcccg gttctactgg ttctgggcag tatcggcctg
      481 tattatcttt atcgaaacgg cggctggtat tttcgctgga agggcctgag ggcggcactc
      541 tggggatggt ttgcgcttat caacacggcg cttctgatcc tgttcacatt caactatgct
      601 catcgcggcg caatcgagcc gatgatttat ctcaaccgac aggaaaaagt tgaaggagtt
      661 ttatttgaca caagcgagcg taaaaaatgg ctgccctatt catactggaa ttaccgcaaa
      721 ccggagtcgg taaaactgac accgcaatac agtctccagg aagcaatcaa tgctggtgag
      781 gtcaaacctg agaatccgcc taaatatata gttgtatttt ccgacggaag gccggatcgg
      841 tatattgaaa aatatgatac atatctgggg gattatgaga ttgtttttca tggtaaacca
      901 tcattgatgg actttattct taatagattg aaccccaaat ataatcatct gaatgaatcc
      961 tgggtgctgg aattccgaac cacgcaggac tgattgttac aaaatccaaa aacgtttccg
     1021 aaattgctta agcccgccgc agggtgcagc gggctcataa atgcaaattt aattatgatg
     1081 acccgaatgg gcagcaatcg ccattgtacc agttggggga gccgggtgag catgtcgagg
     1141 gaggaccacc gccaacgaaa acatagttta ttacccagac agcatcggag acattaactg
     1201 atgcgtccgt gttggcgtct ccgcaggcaa gcacaggctg cggttcatct ccaccaacga
     1261 agacgaaatt tattatccag actgcatcgg agacattgac attggtatca ttattggcat
     1321 ccccgcagag tccctgacag ggaccgccgc ttgttgcaaa gatctgccag aagccatgtc
     1381 tgagcttgta attcgttgat gtgccctgat cgaccgcagt ctggctgaca gtgcccttga
     1441 gaccgtaatt ggttgatgaa ccctcagtgc caccgttgga caaaacctgc cagttgatat
     1501 cttcaccagc tttcccacct tcagctgtca caactgaagc ccagatgatt atgaagcagg
     1561 ctgtgaccaa acctataata tatttatttt ttttcattcc cgctctctcc tctcatcgat
     1621 ctcgcgtctg aattctgcat tcgttgaata ctcggttgag cgagtaacat caaatccgaa
     1681 ggcctccgga ttgatataat agcccttttc ttccgctgtc ttttcggatt ccaccgttac
     1741 cggatgtgat tccgcaaaag catcatggcg aacaccggta acctgccagg agacctcgat
     1801 atctggttcg ctggtagcga ctagaaattg gttattcgat attttctctt ttataattgc
     1861 ctgtgcgaat gagccaatca cggtcaattg atagcggaag tccttgttta gcgattcgaa
     1921 gtagtcggga agctggacaa tggcttcgcc attggcattg gtggttacat ttccgctgta
     1981 tacgttcagc atttcgggcg aattgatagt cgcgtgttcc agcactctgt tctcaggatc
     2041 ggtgggatgg tcaattctca tcgtgctctt gctgttgtcg aatgttccag tgactctgca
     2101 gttgccgctg aaatatcctg cccagttagt tgtagccgaa ccaccagagg catatattcc
     2161 aaaggcggta gcaccgtcgt aggcagttcc gcggaggcca taggtttctc cggttgtgat
     2221 attatagttt tgcgcctcac tgaatgcata tacacctaga cggctcgcac catccgacct
     2281 tgcgtacccg tataccgcat aacagtttcc cgccaccccg gctataggat gttgtgcact
     2341 gctgtatatt ccatatagat cgccggcatc ctgattagta acctcagttg cctggccaaa
     2401 ccatgaaccc gttgagttaa tgtcccaggt gatttggagc tttctgttaa aaaggacctc
     2461 tcctattccg actgtgttat catccccaat ttccatgacg gttctgttac gcagtatatt
     2521 tccatcatca tagacctgga aaaactccag accggcaccc gcctcctccc tgatgcgcca
     2581 gcgatgagaa ccacccctgc ggtaggctat ctgggcatcg ttcatagtcg attctccgtt
     2641 aaaataattg cccatcgtaa atgtattgcc agcattcagc actgccgctg taccactgat
     2701 ctttgtcggg gcgatatcgg ctgaggcgct tatatcattg tcaacaatag agttattcag
     2761 gatgtgatct gatgttatgc cgcctgaggc gacccccaga gttacatcgc ccgacgttgc
     2821 gcccccgctc agaccgctgc ctgccggggt gttcaccgct gtgatgtcgc caagcccggg
     2881 gtaattcaga attccatcaa tggtaagatc gccggtaaag cggccgtcgc cgtcaaaata
     2941 tccggcataa cctccagcgc atactccata tacagccccg ccatcaccga tattccttat
     3001 cctcaatgcc ggattaaaat catccgcagt ttccaggtcg gcgggcagag taagaaagtc
     3061 agcaattgcc gcatgaaaag cataggcagt cgatgttaat ttggtcctgg gaaaaatttc
     3121 cggatcggca ccgacagtta tgcccagata acgggttgtg tcagcgacaa acaaatcagc
     3181 cggaaaaggc gtgctcgaac cgagcatata agtaaacagg ccatccgtga cctgtacggc
     3241 cttgacaccg ctgctccaga gcaggtcggt tccggccgca ctgccgtaga tgttgaaaat
     3301 aatgttatag acgccatctc cgaccgggtc cccggcatca tctgtcaggc agccctggta
     3361 gttgatggag tgcggaacct cggcactcaa tggcattgaa gctaaaataa gaaatgatac
     3421 aattgtgatt agcatgatgt gtctcatgac gacctccctc taaaatatta tagaacgtag
     3481 agtggggaaa gaggtaataa tcaggcttga gcggtcaccc ccacaaattt tctaaataca
     3541 gtcaagccgt agcctggtaa gatataaata aattattata tcagatcgaa ttgtcaacta
     3601 aaaaggaatt ttcgatcaat atgaatctaa aaaaatgcct gctcagaaat gagcaggcat
     3661 tctaatggca tgaagcattt ttacgagccg aatgggcagc agtcgccgtt gtaccagttg
     3721 ggcgagccgg gtgagcatgt cgagggaggg ccgccgccaa cgaagacata attaatgagc
     3781 caaaccgcat ccgaaatgtt tacagtcgca tcattattgg catcgccaca ggccaataca
     3841 ggctgaggtt cctcaccgcc aataaagaca aagttaatga tccagactgc atcggagaca
     3901 ttgacattgg ca
//
LOCUS       DSEB01000111            3344 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003237, whole genome shotgun sequence.
ACCESSION   DSEB01000111 DSEB01000000
VERSION     DSEB01000111.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003237"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..2747)
                     /locus_tag="ENO07_02115"
     CDS             complement(<1..2747)
                     /locus_tag="ENO07_02115"
                     /inference="COORDINATES: protein motif:HMM:PF11028.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HDS00790.1"
                     /translation="MENTNRDRLNWLLAGLVTLVAFIVYFRTMAVSVSLWDCGEFIAC
                     SRILGIAHPPGTPLFIIFGRVFSMLSFLGNDIAHRITFVSVLSSAFSAGFAYLTVSKI
                     ISWWLKEKESFVDRLPMYIGGIVGALFMAFSRTWWTNAVEAEVYGLTLLTSIVAVYLS
                     LVWYEKREQPGSDKYLFLMIFIAMLGVGAHLAAFIVVPAIFLFLLLASPRLLKEPRVW
                     VSMLCGLVLLVAYDYFLWATFIWLVVCIISALGRKDFDWTWMVVFVAFVALSLIIKQR
                     APEAFVWFAVIGLGAFVIRTFTGYSTGSRLWQSLFAVFAIASLIIMDISMELMVWINL
                     IGLPIFLVVAFKTKSYYWRWAMVFTTMMALAFSVQYTVLVRSQHRPRINMTKPDTLTR
                     MNDYLARKQYGQGNMITRMFDRRGELANQFGDYPRMGMLGFWEKQYSNSGIFFLWLFV
                     GLWGLYYAMKTRWKSGLMIFLIVLAGTIGITFYMNFADGTQVMEDPNARLEVRDRDYF
                     WTHGFGMFGWAIGLGIAGFLHSLLMYLRSRKGTQKFIVPVSLLFSLTIFLPAFAIAEN
                     YNYNDRSNEWLPYQFGRDMLMACDTNAILFTAGDNDTYPLWALQEAYGFRQDVKIINL
                     SLANVDWYVLHCKEVFGAPMAIESDQIEVKEIRTERGLERRPEKMYTDPFIGARTYFG
                     SRPRYNQEGQQVGLIEMAQLTIEKVIEASLMAGGSTGNIFSPSEGDTLIMEYPVYFTS
                     LSEPVRNIRFRNQYRHLERVTNLYKIKDTVIVGVTPPPAGPEPFTYNINRSHELLTDV
                     FIFDGMGDPEYARGEFTAMMVHRYYASEYDMIIDSLVGRSDTSRLSELLDVAIQNTPE
                     HPDWPEWKAIRDSLEGVPESKLTEYQQEYVDYMNKLIQIHPYNFYYRQFVSEVLFDMD
                     R"
     gene            2844..3095
                     /locus_tag="ENO07_02120"
     CDS             2844..3095
                     /locus_tag="ENO07_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00791.1"
                     /translation="MFVWCAILFLLGIAAFLDSLFSYGDIFRKVNSVLFMLVSLGLLV
                     RTTTKMRMRRLEGYLEKIDELEHRINELRAEKTKSATPR"
BASE COUNT          938 a          830 c          787 g          789 t
ORIGIN      
        1 atcctgtcca tgtcaaacag aacctcggaa acaaactgcc ggtaatagaa attataggga
       61 tggatttgta tcaatttatt catgtaatcg acatattcct gctgatactc ggtcaatttg
      121 ctttcgggca cgccttcaag actgtccctg atagctttcc actcgggcca atccggatgt
      181 tccggtgtgt tctgaatggc cacgtcgagt aattcagaca gacgagaggt gtcgcttctc
      241 ccgaccaggc tgtcaattat catatcatat tcggaagcgt aataacgatg aaccatcatg
      301 gcggtgaatt caccccgtgc atattcggga tcgcccatgc cgtcaaaaat gaagacatcc
      361 gtgagaagtt catgagagcg gttaatattg tatgtaaacg gctccgggcc ggcgggcggc
      421 ggagttaccc ccacaatcac ggtatccttg atcttataga gattcgtcac tctctcaagg
      481 tgcctgtatt gattcctgaa tctgatattt ctgaccggtt cgctcaggct ggtgaaatag
      541 acagggtatt ccattatgag cgtatccccc tcggagggac tgaatatatt accggtggag
      601 cccccggcca tgagagaagc ctcgataact ttttcgatgg tcaactgtgc catttcaatt
      661 aagccgacct gctgcccctc ctggttatac ctcggcctgg agccgaagta ggttctcgcg
      721 cctataaatg gatcggtgta catcttctcg gggcgccttt caagacctct ttccgtcctg
      781 atctccttca cctcgatctg atcgctttca attgccatgg gtgcgccgaa aacttctttg
      841 cagtggagca cataccagtc aacattggcc agggaaagat taataatctt tacatcttgt
      901 ctgaagccat aagcctcctg cagggcccag agcggatagg tatcattatc acctgccgtg
      961 aataatatgg cgttagtgtc gcaggccata agcatatcac gcccaaattg ataaggcagc
     1021 cattcattcg acctgtcatt ataattataa ttctcggcaa tggcgaaggc cggtagaaat
     1081 atggtcaacg aaaataaaag cgagaccggc acaatgaact tctgcgtccc cttcctggac
     1141 ctcaaataca tcagaagcga atgcagaaat cctgcgatcc cgaggccgat tgcccagccg
     1201 aacatgccga agccatgtgt ccagaaatag tcacgatccc ttacttcaag tctggcgttg
     1261 gggtcctcca tcacctgggt gccatcggca aaattcatgt agaaggtaat gccgatagta
     1321 cccgcgagta caatcaggaa gatcataagc ccggatttcc atctggtttt catagcatag
     1381 taaagtcccc agaggccgac gaacaaccag agaaagaata tgccggaatt cgaatactgc
     1441 ttttcccaga aacccagcat acccatacgt ggataatcgc cgaactgatt cgccagctcg
     1501 ccgcgcctgt cgaacatgcg ggtaatcata ttgccctgac cgtattgctt cctggccagg
     1561 tagtcgttca tgcgggtaag ggtatcaggc ttggtcatat taatccgggg acgatgctgg
     1621 gaacgcacaa gaacagtata ctgaactgag aaggccagcg ccatcatagt agtgaaaacc
     1681 attgcccatc tccagtaata gcttttggtc ttaaaagcga caaccagaaa gatcggcagc
     1741 ccgatcagat taatccagac catcaattcc atcgagatat ccatgattat caatgatgca
     1801 atagcgaaaa cagcaaagag tgactgccac aatcgtgagc ccgtggaata gccggtgaaa
     1861 gtccgaatca caaatgcgcc caggccgatt acggcaaacc agacgaaggc ctcgggggca
     1921 cgctgcttta tgataagact gagtgcgaca aaggccacga atacgaccat ccaggtccag
     1981 tcgaaatcct ttctgcccag ggcgctgatg atgcagacca ccagccagat aaatgtagcc
     2041 cacagaaagt aatcataggc gaccagtaga accagcccgc ataacatcga gacccagacc
     2101 cgcggctcct tgagcagacg gggtgatgcc agaagcagga agagaaagat agccggaacc
     2161 acaataaatg ccgccagatg tgctcctact cccagcatcg cgataaaaat catcagaaat
     2221 agatacttat cgctgccggg ctgttcgcgc ttttcgtacc agaccagcga gagatagact
     2281 gccacgattg atgtcagaag cgtcaggcca tagacctcgg cctccacagc atttgtccac
     2341 caggttctcg aaaaagccat gaacagggcc ccgacaattc cgccgatata catcggcagc
     2401 ctgtcgacaa agctctcttt ttcttttagc caccagctga taatcttgga tactgtcaga
     2461 taggcgaaac cggccgagaa agcggaggaa aggaccgata caaaggtgat cctgtgagca
     2521 atatcgtttc ccaggaatga cagcatggaa aagacccgac cgaatataat aaacagcgga
     2581 gtcccgggag gatgggcgat gccgagtatc cgggaacatg cgatgaattc cccgcaatcc
     2641 cagagagaaa cagacacagc cattgttctg aagtaaacaa taaaagctac aagagttacc
     2701 aatccggcca ggagccagtt cagtctgtct ctattcgtat tttccatccg atcgatcctt
     2761 tcctaatctt tcacgaaaat ttaccgataa ggatatataa aacaggacaa aatatcaact
     2821 caaatctcaa tcagtgggta agtatgtttg tatggtgtgc catattattt ttgttaggaa
     2881 ttgcagcttt tctcgacagc ctttttagtt atggcgacat attcagaaag gtcaactcgg
     2941 ttttattcat gcttgtatcc ctgggccttc tggtgcgcac cacgaccaag atgaggatgc
     3001 gcaggcttga gggatatctg gaaaaaatcg atgagctcga gcacaggatc aatgagctga
     3061 gggctgagaa aaccaaatcc gcaactcccc gttagagatt taaaaatcgg acagctcagc
     3121 aggaataaat cagaattcct tggtccggaa acttctctat tttataaaag ctgggataat
     3181 cctggttcct gaatcgaatt gggtattctg ccgaatttta agaactttcg atcttgcgaa
     3241 gctgacggcg ggcatattcg taaagtacct tgttggggta attttccagg attacgcggt
     3301 aggcttcacg agccttctcg tgatcattga gatggtaata ataa
//
LOCUS       DSEB01000112            3963 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002219, whole genome shotgun sequence.
ACCESSION   DSEB01000112 DSEB01000000
VERSION     DSEB01000112.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002219"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..345)
                     /locus_tag="ENO07_02125"
     CDS             complement(<1..345)
                     /locus_tag="ENO07_02125"
                     /inference="COORDINATES: protein motif:HMM:PF04093.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00792.1"
                     /translation="MLGKIAVFAALVIIAIIFQTFFASLISIDNVMPDIYVVLVIYLT
                     LTQGLAYGAVFGFITGVIEGSADPGLLGLSAVLKVVLAVIVYFMSIRIRLESNFARIL
                     TVLISVAAHNVLY"
     gene            complement(346..1149)
                     /gene="mreC"
                     /locus_tag="ENO07_02130"
     CDS             complement(346..1149)
                     /gene="mreC"
                     /locus_tag="ENO07_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDS00793.1"
                     /translation="MTAILRKLFGENKEVGLAIILAALSFVILILPLDLKRPMAKYTY
                     LVTLSPFQGVSEKIKRLAETTQTNKWLREQLLKRELELILVREALEQNERLRDSLGFR
                     QVGGFTVIPGELKQIDPKRRDNALIVSVREDHEVAPDMAVVSLSGLVGKTTHVMINTV
                     TVELLTSPNCRVAARDQNTRTMGIIRWESGNDLLLDNVPVRDPVNVGDTVISSGLGGL
                     FPEGLPIGVVSEVELPERGFFKDIKVRPFVDFNRIDELFILKQPKETEG"
     gene            complement(1158..1610)
                     /locus_tag="ENO07_02135"
     CDS             complement(1158..1610)
                     /locus_tag="ENO07_02135"
                     /inference="COORDINATES: protein motif:HMM:PF00359.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDS00794.1"
                     /translation="MKLSKFTEEELITFNLKGEDKEDIIEELVDLAAKSKLVRDRDEL
                     LKDIIERENLVTTGVGYGVAFPHAKTKAVKGIVIAFGRSDKGIEFDAMDKKPVYLFFL
                     IAAPEDAIGAHLNVMARLSFLMKSEENREKLMKVNNQGELLAILDSVE"
     gene            complement(1725..2687)
                     /locus_tag="ENO07_02140"
     CDS             complement(1725..2687)
                     /locus_tag="ENO07_02140"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_952454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HDS00795.1"
                     /translation="MSAGFVLEFEKPIVELERKIAEMKEFASGENVELSREIESLEQK
                     LDRLADEIYSKLTRWEKVQLARHPRRPYTLDYINALCHDFVEIHGDRGFADDKAVVCG
                     FGRFHTYKVALVGQQKARDTKGKLMRNFGMMHPEGYRKALRVMRLAEKFELPILIFID
                     TPGAYPGIGAEERGQAEAIARNIREMFRIKVPIIISIIGEGASGGALGIGVGDVVLML
                     ENSWYSVISPEGCAAILWRDQAKASEAAEALKLTAQDMLELEVIDKIIPEPRGGAHRN
                     PQGVYELLGKEILTQLQYFNSLSKEQLLEQRTQKFRRMGEVLQI"
     gene            complement(2684..3748)
                     /locus_tag="ENO07_02145"
     CDS             complement(2684..3748)
                     /locus_tag="ENO07_02145"
                     /inference="COORDINATES: protein motif:HMM:PF00953.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDS00796.1"
                     /translation="MQECPEILGISLILLVSAIVLSSGLTFSLIKLSRLKKIYDYPNG
                     RKIHRRPTPFLGGAAIFAAFWIIFSLIGVYCREAMFGGEQFFLAFLLTSTLIFITGLI
                     DDFFDLRFYIKLLAQIAAALILIAFDFHIINLYVPFWRSFELGWAAYPVTILWVVLLT
                     NGINLIDGMDGLASLVGITICIGLLIISSFLDILLIFVIAVILAGCLIGFLIYNKPPA
                     KIYLGDSGALFMGFVFAVAAIVCPIKSFTAVSMFVPLVAVGLPLLEIVTTVVRRIWSG
                     KKIYQPDTRHIFHYLMNFGYSEKSILLILGTISLAFNAFIPALFIFDRRQVFSIFVLF
                     LISLFILFFILKLVKGAERT"
BASE COUNT         1061 a         1035 c          907 g          960 t
ORIGIN      
        1 atataatacg ttatgagccg ccactgatat tagtactgtc aaaatgcgcg caaagtttga
       61 ctccagcctg atccgaattg acataaaata tacgattact gccaggacaa cttttaagac
      121 cgcgctcagg ccaagcagac cggggtcggc ggagccctcg ataacaccgg ttatgaaacc
      181 gaacactgcc ccgtaggcaa gcccttgtgt aagagtgaga tagattacaa gcacgacata
      241 tatatctggc attacattat cgatggaaat caggctggcg aaaaaggtct gaaaaatgat
      301 ggctattatc accagagcag caaaaaccgc aatcttgccc agcatctacc cttccgtttc
      361 ctttggttgc ttcagaatga aaagctcgtc aatccgattg aaatcgacaa agggtctgac
      421 cttaatatct ttgaagaagc cccgttccgg aagttccacc tcgctgacca cgcctatggg
      481 aaggccctca ggaaaaagcc cccccagccc ggagctgatg acggtatctc caacgttcac
      541 gggatcccgg acaggcacat tatccagaag cagatcatta ccgctctccc acctgattat
      601 tcccatggtg cgggtattct ggtcacgtgc cgccacccgg cagttgggac tggtcaatag
      661 ttccacagtt accgtgttta tcatgacgtg ggtggtcttg cccaccaaac cggataaact
      721 gaccacggcc atgtccggag ctacttcatg atcctcgcgc accgacacaa tcagagcatt
      781 atcgcgccgt ttcggatcaa tctgcttcag ttctccggga attaccgtaa atccccccac
      841 ctggcgaaat ccaagagaat cccgcagcct ttcattctgc tcgagcgcct cacgcaccag
      901 gataagctcc agttctcttt tgaggagctg ctcccggagc catttattgg tttgagtggt
      961 ctctgcaagc ctttttattt tttcggagac cccctggaaa ggcgagaggg ttacgaggta
     1021 tgtgtacttt gccatcggcc gtttaagatc gaggggcaaa atcagtatca caaacgacag
     1081 ggctgccagg ataatggcca gccctacttc cttgttttct ccaaatagct tcctcagaat
     1141 tgctgtcata aatccattta ttcgactgaa tcaagaattg ccagcagttc cccctgatta
     1201 tttactttca tgagtttctc acgattctcc tcggacttca tcagaaacga aagacgtgcc
     1261 atgacattca gatgggcgcc gatagcatct tcaggcgcgg cgatcaggaa gaaaagatag
     1321 accggcttct tatccatggc atcaaactcg attcctttgt cggagcgtcc gaaagcgatc
     1381 acaatccctt tcaccgcctt ggtcttggca tgaggaaagg cgacaccata tcccacaccg
     1441 gtggtcacaa gattctcgcg ctctataata tctttgagaa gttcatcgcg gtcgcggacg
     1501 agtttcgact tcgctgccag atcaaccagc tcctctataa tatcttcttt atcctcgccc
     1561 ttgaggttga aagtgatcag ttcttcttcc gtaaatttag agagtttcat gcagtctcct
     1621 tacatgctta ttggaattca tattagccaa taataagcta catatctgaa gccgtttcaa
     1681 tatggcatga gatcaaatat tcgttaacgg cgcctccttt atgtttatat ctgcagaacc
     1741 tcacccatgc gccggaattt ctgggttctc tgttcaagca attgctcttt tgaaagagag
     1801 ttaaaatact gcagctgcgt caggatctct ttccccagaa gttcatagac tccctgggga
     1861 tttctatgcg cgcctccgcg gggctcggga ataatcttgt cgataacttc cagctccagc
     1921 atatcctggg cggtcagttt cagggcctcg gctgcttccg aggccttggc ctggtcacgc
     1981 cacagtatcg cagcacagcc ctcaggtgag atcactgaat accaggagtt ttcgagcatc
     2041 aggacgacat cccctacacc aatgccgaga gcacctcccg aggcgccctc gccgattatg
     2101 gaaattatga tcgggacctt gatccggaac atctcgcgta tatttcgggc gatcgcttcc
     2161 gcctgccccc tctcttccgc gccaataccc ggatatgctc cgggagtatc gatgaagatc
     2221 agaatgggaa gctcgaattt ttcagccagc ctcatcacgc gcagggcctt acggtatccc
     2281 tcgggatgca tcataccgaa attccgcatc agcttgcctt tggtatcgcg ggccttctgc
     2341 tgcccgacca gggcgacctt gtaagtgtgg aacctcccga agccgcagac caccgcctta
     2401 tcatcggcaa acccgcgatc tccgtggatt tccacaaaat catggcacag tgcatttata
     2461 taatccaggg tataggggcg tctggggtgg cgcgcaagct ggactttttc ccagcgcgtc
     2521 agctttgagt atatctcgtc agcaaggcgg tctagcttct gctccaggct ctcgatttca
     2581 cgcgaaagct cgacattttc acccgaggca aattccttca tctcagcaat tttacgctcg
     2641 agttcgacta tcggcttttc aaattccaat acaaatcctg cgctcatgtt ctttcagcac
     2701 cttttaccag tttcaatata aagaataata tgaacaatga tatcaaaaac aaaacaaata
     2761 tagaaaaaac ttgcctgcgg tcaaatataa aaagcgcggg tatgaacgca ttgaatgcca
     2821 atgagatcgt acccaagatc aagaggatac tcttctcgga atagccgaaa ttcataaggt
     2881 aatgaaatat gtgtcttgta tccggctgat aaattttttt gccgctccag atcctgcgaa
     2941 cgacggtggt gacaatctcc aggagtggaa gccccacggc aacaagcggt acgaacatgg
     3001 aaacagcagt aaagctcttg atcgggcaga cgatagcggc cactgcaaaa acaaatccca
     3061 taaaaagcgc gcccgagtca cccagatata ttttggccgg gggtttgttg tagatcaaaa
     3121 atccgatcag gcagccggcc aggataactg caatcacgaa tatcagcagt atgtccagaa
     3181 aggaggatat tatcaagaga ccgatacaaa tggtgatacc caccagcgat gccagaccgt
     3241 ccatgccgtc aatcagattg atcccattcg tcagaagtac cacccagagg atggtcacgg
     3301 gataggcggc ccagcctaac tcgaatgacc tccagaaggg gacgtagagg ttgataatat
     3361 ggaaatcgaa tgctatcagt attagcgcgg cggcaatctg ggccagaagc tttatataga
     3421 acctgaggtc aaaaaaatca tcgatcagcc cggtgataaa tatcagcgtt gaagtcagca
     3481 ggaaagccag aaaaaattgt tctcctccaa acatggcctc ccggcaatag accccgatca
     3541 gggaaaagat tatccagaat gctgcgaaga tcgccgctcc ccccaggaat ggagtgggcc
     3601 tgcgatggat cttcctgccg ttgggataat catagatttt cttcaaacgg gaaagtttga
     3661 tgagagaaaa ggtcagcccg cttgacagaa cgatagctga gaccaggaga atgagactaa
     3721 tgcctaatat ttcgggacat tcctgcatag gctatgataa tctgaaaaat aaaattaacg
     3781 gtcaacataa aaaagagccc caggttgggg aggattttgc ccgggtacca tttcaagaaa
     3841 tattccatgg ccgagatgtg atgatgcata atccgtcggg ccggactttt ggatgatgcc
     3901 tgcagctgat gtatcatcac cgcctcggga acataataac aataccttcc ttctctattc
     3961 aac
//
LOCUS       DSEB01000113            4288 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001883, whole genome shotgun sequence.
ACCESSION   DSEB01000113 DSEB01000000
VERSION     DSEB01000113.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001883"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1200
                     /locus_tag="ENO07_02150"
     CDS             <1..1200
                     /locus_tag="ENO07_02150"
                     /inference="COORDINATES: protein motif:HMM:PF13458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDS00797.1"
                     /translation="AEEIVDNLEVLYPDYAGDRRLDALRQKIVQFASQKAILALFVPL
                     SGDWARFGRMMSNAVDLALEEYRKQPGIEVEKKTFDTFGNMIAAAIDAKKLMDDPVSA
                     IIGPLSSGEAVGVAAFSEIGNIPVIAPTASEKGLTSISDMFFQLTPTSERMGEALAEF
                     VARDIGFDSVAILAPLDNYGKQITDGFVQTAIANGATIFYQTFYPRGTRDYRRFLLEL
                     KQELLPDTFVAEIFLNDEGDTLELEEIPVHIPAIFMPSYESELKLLLPQLRFYKISTI
                     LLGSDSYGESEIVEMKESRDNPVMFVSNTLTLPEDTLWLRFNYLYQTKFDEAPEQVAA
                     ATYDAANIVLECVSKGLYTSDEIWQCVMEKKHYQGASGKIRFSPQRENIFVPVYFLSE
                     GEIIRLE"
     gene            1197..2036
                     /gene="larE"
                     /locus_tag="ENO07_02155"
     CDS             1197..2036
                     /gene="larE"
                     /locus_tag="ENO07_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HDS00798.1"
                     /translation="MRNELLEKYEKLKDIISGFNSAIVAYSGGLDSAFLLYAVVDTLG
                     AENVSAVTADSPSYPRSELEKAQEFAWSLGLGERHRIIKTEELDDPNYAKNPVDRCFF
                     CKQELYGKLSDPSLFDGHDVIFDGFNADDVGDFRPGRKAAEKYNVRSPLFEAGLGKAD
                     LRELASHFGLEVWDKPQMACLASRIPYGSPVTKEKLAQVEKAEEYLRSLGFMQLRVRH
                     HDRLARIELPLADIKKVYNQELQRKIADEFRKLGFVWVTLDLAGFRSGSMNEAIKRSD
                     EDV"
     gene            2029..2250
                     /locus_tag="ENO07_02160"
     CDS             2029..2250
                     /locus_tag="ENO07_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00799.1"
                     /translation="MFEKKKVAMKIQMPDGNVKEVSFEELALSNNLASQALVRLLIKK
                     GVITGEEFMESMQEMQKSHYKDPNAPEEK"
     gene            2410..2955
                     /locus_tag="ENO07_02165"
     CDS             2410..2955
                     /locus_tag="ENO07_02165"
                     /inference="COORDINATES: protein motif:HMM:PF01797.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDS00800.1"
                     /translation="MIANSGLINIFIDTMSNLRRYYSQGQIYFVTAVTYNRMPILVEE
                     IGIFRALIKSKILEKRSNVIALCVLPDHFHMMIDPQRNDLSLLIKSFELSFSKLYMYK
                     HDLKKHRIWQYRFWDHIIRDQRDMNNHINYIHYNPVKHGFTCNPFEWQYSSIHKFYRQ
                     GYYSKGWGINKKIEFEGNYGE"
     gene            complement(2996..3850)
                     /locus_tag="ENO07_02170"
     CDS             complement(2996..3850)
                     /locus_tag="ENO07_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00801.1"
                     /translation="MAIIRTIILVVILPILALGQTSREEDKLSIYEIVQPDGFFGTGF
                     MIEDSLMGQMLITCRHIIQDENGDFFDKIFVRRNKLLKTKQVVSDTVGFYVHLKDKEN
                     GETRNLFYSHPDPNIDLAIIPVIHPDQPLKNRKPTYGFDSRLVLSRMGLDSMGVEEGT
                     DVEIIGFSLTSSLYIDRINYHFSRFGKIGLFTSDDLELTIDKQPRKANFMLLSMSVKI
                     GDSGSPIIAHIGGRRYIIGIVTAVVPDIAYGVGYPSYYIYDLLEQIRADYIEKLGLAN
                     KSKQVKSL"
     gene            4153..>4288
                     /locus_tag="ENO07_02175"
     CDS             4153..>4288
                     /locus_tag="ENO07_02175"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="transforms a conserved lysine residue of initiation
                     factor 5A into deoxyhypusine; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HDS00802.1"
                     /translation="MKNPKVEAIEVKSSRKISGLMEAMAKTGFQGKSLARSVEVFERM
                     I"
BASE COUNT         1156 a          955 c         1074 g         1103 t
ORIGIN      
        1 gccgaggaga tagtagacaa cctcgaggtc ctttatccgg attatgccgg cgacaggcgt
       61 ctggatgccc tgaggcagaa gattgtgcaa tttgcctctc aaaaagcgat cctcgcactc
      121 tttgtcccgc tttccgggga ctgggcaagg ttcgggcgta tgatgtctaa tgctgtcgat
      181 ctggcactgg aagaataccg caaacaaccg ggaattgagg tcgagaaaaa gacctttgat
      241 accttcggca atatgattgc ggccgctatc gatgccaaga agctgatgga tgatccggtc
      301 tcggccataa tcgggccgtt atcatcggga gaagctgtgg gggttgccgc cttttccgaa
      361 atcggcaata ttccggtcat cgccccgaca gcctcggaaa aagggctgac ctcgatttcc
      421 gatatgtttt tccagcttac tcccacgtct gaacggatgg gcgaggcgct ggcggaattc
      481 gtggcccgcg atatcggctt cgattcggtg gccatcttgg cccccctgga taattatggc
      541 aagcagatta ctgacggttt cgtgcagacc gctatcgcaa acggtgccac catattctac
      601 cagacattct atccgcgcgg cacacgggac taccgcaggt tccttttaga attgaaacag
      661 gagctgctgc cggacacatt tgtggcggag atcttcttga acgacgaggg tgataccctg
      721 gaactggagg agataccggt tcatatcccg gctattttta tgccctcgta tgaatccgag
      781 ctgaagcttc tcctgcccca actgcgcttc tacaagatca gcacaattct actcggctcc
      841 gattcttatg gcgagagcga gattgtggaa atgaaggaaa gccgggataa tccggtgatg
      901 tttgtgtcca ataccctgac tcttccagag gatacactct ggctcagatt taattatctt
      961 tatcaaacca aatttgatga ggcacccgag caggtggccg cggcaacata cgatgctgct
     1021 aacatcgtgc tcgagtgtgt gagtaaaggt ttatacacct cggatgagat ctggcaatgc
     1081 gttatggaga aaaagcacta ccagggcgcc tcggggaaaa tccgcttcag ccctcaaaga
     1141 gaaaatatct tcgtgcccgt atattttctg tcggaaggag aaatcataag gctcgaatga
     1201 gaaacgaact gcttgaaaaa tacgagaagc tcaaagatat aatatccggc tttaactcgg
     1261 cgattgtggc gtactcaggc ggtcttgact ccgccttttt actttatgcc gtcgtggata
     1321 ccctgggggc ggagaatgtt tctgcggtta ccgccgattc tcccagctat cccagaagcg
     1381 agcttgaaaa ggcgcaggag ttcgcctggt ccctgggtct gggagagcgg caccgaataa
     1441 tcaaaaccga ggagctggat gatcccaact acgccaaaaa tccggttgac cgctgtttct
     1501 tctgcaagca ggagctctac ggcaagttgt ccgatccgtc gctgtttgac gggcatgatg
     1561 ttatttttga cgggtttaat gccgatgatg tcggcgattt tcgtcccgga cgaaaggcgg
     1621 cagagaagta taatgtgcgt tcacctctat ttgaggccgg tctgggtaag gccgatcttc
     1681 gggagttagc cagccatttt gggctggagg tctgggacaa gccgcagatg gcctgccttg
     1741 cgtcacgcat tccctacggc tcgccagtca cgaaagaaaa gctggcgcag gtggagaaag
     1801 ccgaggagta tctgcgttca ctgggattca tgcagctgcg ggtgcgtcac catgacaggc
     1861 tggcgaggat agaattgccg cttgcagata taaaaaaggt ttacaatcag gaattacagc
     1921 gtaaaatagc cgatgagttc cgcaagctgg gatttgtctg ggttacgctt gacctggccg
     1981 gattccgttc cggaagcatg aacgaggcca taaaaaggag tgatgaggat gtttgagaag
     2041 aagaaagttg cgatgaagat acagatgccc gacggcaatg ttaaagaggt gtcgttcgag
     2101 gagctggcgc tctccaacaa cctggcctcg caggcattgg tcaggctttt gatcaagaag
     2161 ggtgttatca ccggcgagga gttcatggaa tcgatgcagg aaatgcagaa atcccattac
     2221 aaggatccga atgccccgga agaaaaatag ctctcattag agaaagccaa atttcgtgac
     2281 cggcggatgt catcattcgc cggttttttt ggattgcggc gaaagaaatt gtaggtcaaa
     2341 agtctgtgac ttttgacatt gtcaaatctc acagggatct gacctacgag atgtccatta
     2401 aatcttaaaa tgattgcaaa ttctgggtta ataaatatct ttattgacac catgtcgaat
     2461 ttaagaagat attattcaca aggtcaaatt tattttgtca ctgctgtaac gtataatcga
     2521 atgcctatct tggtcgagga gatcggaata ttcagagcct taattaaatc aaaaattctt
     2581 gaaaagaggt caaatgtgat tgcattatgc gttcttcccg atcattttca tatgatgata
     2641 gatccccaaa ggaatgatct ttctttactg ataaagtctt tcgagctctc gttctcaaag
     2701 ctatatatgt ataaacatga tttgaaaaaa catcgtattt ggcaataccg gttttgggat
     2761 catataatta gggatcaaag ggatatgaat aatcatatta attatattca ctataatccg
     2821 gtcaagcatg gcttcacctg caatccgttt gaatggcagt attcaagcat ccataagttt
     2881 tatcgacaag gatattattc caaaggatgg ggaataaata agaaaattga atttgaaggg
     2941 aattatggag aatagtcgct cgtcaaaagt ctgtgacttt tgacattgtc aaatctcaca
     3001 gagatttgac ctgcttactt ttattcgcca atccaagctt ctcaatataa tctgcccgga
     3061 tttgctctaa taaatcatag atgtaataag acgggtagcc gacgccgtag gcgatatcgg
     3121 gaaccaccgc agtcacgatt ccgattatgt agcgccttcc gcctatatgg gcgatgatgg
     3181 gggagccgga atctccgatc ttgacagaca tcgacaagag cataaaattg gcctttcgcg
     3241 gctgtttatc aatggtcaat tccaaatcat cgctggtaaa cagaccgatc ttaccaaagc
     3301 gcgagaaatg ataattgatc cggtcgatat agagcgaact ggtgagcgaa aagccgataa
     3361 tctctacatc agtcccctcc tccacaccca tactatcaag acccattctg cttaatacaa
     3421 gtcgggaatc gaaaccatat gtcggtttgc ggtttttgag cggctggtcg gggtggatca
     3481 cgggaatgat cgccaggtcg atattgggat cgggatgcga ataaaacagg ttccgtgtct
     3541 cgccgttctc cttgtcctta agatggacat aaaatccaac agtgtcggag acgacctgtt
     3601 ttgtcttgag aagcttgttt ctcctgacaa aaatcttgtc gaagaaatcg ccgttctcat
     3661 cctgtataat atgacggcag gtgatgagca tctggcccat caggctgtcc tcgatcatga
     3721 agccggtgcc aaaaaagccg tccggctgaa cgatctcgta tatcgacagc ttatcctctt
     3781 cccgggaggt ctggcccagc gccaaaatgg gcagaattac aactaatatg atggttctaa
     3841 ttatagccat agttaatata tttatactgc tgaaaaaaac cacagtatgg gcctaaagtc
     3901 aatgtttttc ggtatttgtt tattaatgtc gggagcccag cataatatct gaatgacagt
     3961 gctcggagta cgtccttgtg ctccgcgttc atattcccct tcgccttttg aataccagac
     4021 cgggtgtacc gggagctaag cccagcgcca tattattgtg agagaaactt ttctttccat
     4081 ttatcgctca aattcttata ttaaccaccc aaaaatcgta aatccgaaaa tcgaaaagat
     4141 ggggatgtga aaatgaaaaa tccgaaagtt gaagccatag aagtaaaatc gagtcgcaaa
     4201 atctcgggcc ttatggaggc catggccaaa accggattcc agggcaagag cctggcgcgt
     4261 tcggtcgaag ttttcgagag gatgatca
//
LOCUS       DSEB01000114            3850 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002352, whole genome shotgun sequence.
ACCESSION   DSEB01000114 DSEB01000000
VERSION     DSEB01000114.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002352"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(1353..>3850)
                     /locus_tag="ENO07_02180"
     CDS             complement(1353..>3850)
                     /locus_tag="ENO07_02180"
                     /inference="COORDINATES: protein motif:HMM:PF05592.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alfa-L-rhamnosidase"
                     /protein_id="HDS00803.1"
                     /translation="EYPRFFWNVTGANKQTAYSITAQTDGKIIWQSGKVFSGSMRAAY
                     AGPSLESRQRVEWSVTLWDENDAIGGETSAFFEMGLLDPSDWMAKWITADLEIDKKVR
                     YPVDSFKKEFNTKKTIKRARIYLTACGLYEALLNGTKVGNAELTPGLTDYRKRVQYQT
                     FDVTDHLRAGANSLEISLADGWYRGYIGALSTPNVFGERTKLLCQLEIDYADGTQEVI
                     CSDDSYSWSNDGAIRYADLKNGEIVDAGRKPSYTGQARLDNHPITPTASNNVLIQQHE
                     RFTPKLITSPSGKRILDFGQNLAGYIEFHLQGQKGQKAILKMGEALDHDEFTQVNFQG
                     KMKNIDQKIEFTCSGEEDFYRTKFAIFGFRYALVEGLEEIHPNDFSAIAVYSSMEETG
                     DFACSNELINLLVKNTRWSMKGNFADAPTDCPTRERAPWTGDAQIFCNTASYLMNTAP
                     FFGKWLGDLADRQAEDGKVQCIVPSVGDKVGGMQWMDGCVGWGDASIFIPYRCYQMYD
                     DPEFLRHFYPMMKGFAEFSIRRAAKTFFANWFKKNPYRKYTYDTGQHFGEWLEPEGVE
                     PGNMIVNIILPRPEEATAYFHYQMRLMSETANILGYESDSKRYAEYADGAKKAYSYLF
                     VKENTIKTDRQAKLVRPLALGLLDGEVKKNVEDRLEKVLSKNDWKIGTGFLSTPFILP
                     VLTETGKLDAAFKVLENEEVPGWLFAPKQGATTIWESWEGYDNDGIPKASHNHYAFGA
                     VCEWLFNTVAGINVAGENEFLIAPQFGGTLTHASAKYQSIFGEVSSSWKLKDGIFNLE
                     VDVPSNCTAKIILPNRETHKVEHGHHILTIAAD"
BASE COUNT          945 a          945 c          909 g         1051 t
ORIGIN      
        1 cccagaagtg cttctggacg tactcgatgt aatccttttc tatattgagc gcggtgcaaa
       61 tctaatccgt ctggatgcaa ttgcttattt atggaaagaa atcgaaacga cttgcattca
      121 tttgcctcaa acgcatgcag tagttaaatt gctccgcgca gttatagacg aaattgctcc
      181 cacggtactg ctgattactg aaacaaacgt ccctcacgaa gaaaatgtct cttatttcgg
      241 gaatggcaag gatgaggccc atctggttta ccaattctca ctgcccccgc ttactgccca
      301 tgcactattg acaggttctt ccgcgtattt gcgtgaatgg gccaaatctc ttaaactgcc
      361 ttccgaccaa accagctttt ttaattttac cgcttctcac gatggggttg gggtgcgccc
      421 ggttaccggg atcttacccg aaaaagaact ggaaactttg ctgatgaaag cccaggccca
      481 tggtgggaga atttcctcaa aaaacaactc agatggcaca accagtcctt atgagctaaa
      541 tataaattat tttgatctca tcaacgatcc attctcaaat gaagatcgaa atatccaagt
      601 tgacagattt attgcctcac aggcgatgat gttgatgttg aaagggattc cgggtgtata
      661 tttgcacagc atcgtcggct cacgcaatga ttactcgggc gtccagaaaa gcggtcaggc
      721 tcgttcgatc aatcgggaaa agttaaatta tgaggcactc agtcgagagc ttagagatgg
      781 tgggtcgctg aggaacgccg tttttcggaa atatcagcgc cttctaaaga tcagaatccg
      841 agaaaaagct tttcaccctt ctggcgagca acatgttatc gatgtcgggg actcggtttt
      901 tgccgttatc cgaacatctt taggtagatc tgaaaaaatc atagctctcc agaatgtcac
      961 cgaagaagtt caggaaattg atattgcaga agctattgtt gaaaggtctg agaggcctca
     1021 atgggttgac gtgatctcgg ggaaaagtta cagcatccca tcattggttc tgaatcctta
     1081 tcaaactatt tggctgaaat atccggaata gaatcaggca accatgcgtt tgctgatttg
     1141 attagaccag ctggttccca aatattttga gccgagataa ttctcttgag gaacatcatt
     1201 atatactggg ccggaactga gtcagtgcgc gcaattttgc gtcggctaat taaacgggtg
     1261 ttttccggtc gcgccactcc acccagtata actaggagac tactaaggta gatttgatgg
     1321 ctggagaatg tattaggtgg atattctgat tctcagtcag cagcaatcgt taaaatatga
     1381 tgaccatgct caactttatg cgtttctcta ttgggcagga tgattttggc tgtgcagttg
     1441 cttggcacat ccacttctaa gttgaagatt ccatccttca atttccagct tgacgatacc
     1501 tcgccaaaga tactctggta ttttgcgctt gcatgggtca aggtacctcc gaattgcggg
     1561 gcgatcagga attcattctc tcctgccaca ttgatgcctg ccacagtatt gaacaaccac
     1621 tcgcagactg caccaaacgc ataatggtta tgggatgcct tagggatccc atcattatca
     1681 tatccttccc agctctccca gattgtggtt gccccttgtt taggtgcaaa aagccagcca
     1741 ggaacctcct cgttttctaa tactttgaaa gcagcatcaa gtttgcccgt ctctgtcagc
     1801 acaggcagaa tgaaaggtgt agacaaaaag ccggtgccaa tcttccagtc attcttggaa
     1861 agcacttttt ccagtctgtc ttcgacattc ttctttactt ctccatcaag aagccctaac
     1921 gcaagaggac gaaccagctt tgcctgtcga tcggtcttga tggtattttc tttgacaaag
     1981 agatagctgt atgccttctt ggctccatca gcgtattctg cgtagcgttt gctgtctgat
     2041 tcatacccca aaatattagc ggtttcgctc atcagacgca tttggtaatg aaaatacgcc
     2101 gtagcttcct caggtcgtgg gaggataata ttgacaatca tattgccagg ttccacgcct
     2161 tcgggttcca gccattcgcc aaaatgttgg ccggtatcgt atgtatattt cctgtaaggg
     2221 tttttcttga accaattagc gaagaaagtc ttcgcggccc gacgaatgga aaactcagca
     2281 aaccccttca tcatcgggta aaagtggcgc aagaattcag ggtcgtcgta catctgatag
     2341 cagcggtatg ggataaagat cgaggcgtct ccccaaccga cacagccgtc catccactgc
     2401 ataccgccaa ctttgtcccc gacagatgga acgatgcatt gcaccttccc gtcttccgcc
     2461 tgccggtcag ccaggtctcc caaccactta ccgaagaatg gggcggtatt catcagatag
     2521 ctggcggtgt tgcagaagat ctgcgcatca ccagtccatg gggctcgctc acgggttgga
     2581 cagtctgtgg gtgcgtcggc gaaattgcct ttcatgctcc agcgagtgtt tttcacaagt
     2641 agattgatca gttcgtttga gcaagcgaaa tccccagttt cttccatgga ggaatagacc
     2701 gcgatagcgc tgaagtcatt ggggtgtatc tcctccagtc cctctaccag ggcatagcgg
     2761 aagccgaaga tggcgaactt tgtccggtag aaatcttctt cgcccgagca ggtgaactcg
     2821 atcttctggt cgatattctt catcttaccc tgaaagttca cctgagtgaa ctcgtcgtgg
     2881 tcgagagcct cacccatctt caagattgcc ttttgcccct tttgcccttg taggtggaac
     2941 tcgatatatc cggcgagatt ttgcccgaaa tcgaggattc ttttgcctga tggggaggta
     3001 attaacttgg gcgtgaagcg ctcatgctgc tgaatcagca cattgttgga tgctgtaggt
     3061 gtaattggat gattatccag cctcgcctgg ccggtataac tcggcttccg acctgcatcc
     3121 acgatctctc catttttcaa gtcggcatat cgaatagccc catcgttact ccaggagtat
     3181 gagtcgtcag agcagatgac ctcttgcgtg ccatctgcat aatcgatttc aagctggcaa
     3241 agtaatttgg tccgttcgcc aaaaacattg ggagtgctta acgctccgat atagccgcga
     3301 taccagccat cggcgagaga tatttcgagc gaatttgctc ccgctcgaag atggtcggtc
     3361 acatcgaagg tctgatactg cacgcgcttg cggtaatcgg tcaggccagg ggtcagttct
     3421 gcgttgccga ccttggtgcc attcagcagg gcttcataca aaccacaggc ggtcagataa
     3481 attctggcgc gcttgatggt tttcttggtg ttgaattcct tcttaaagct atcaaccgga
     3541 tagcgcactt tcttgtcgat ttccagatct gcggtgatcc acttggccat ccagtcagag
     3601 ggatcgagca gccccatctc aaagaaggcg gatgtttcac cacctattgc atcgttctcg
     3661 tcccagagag tcaccgacca ctcaacgcgt tggcggcttt ccaatgaagg cccggcataa
     3721 gcagcacgca tactgcccga aaacactttt ccactctgcc agatgatctt gccgtccgtc
     3781 tgagcagtga tgctgtaggc agtttgtttg tttgccccgg taacgttcca gaaaaagcgt
     3841 gggtattcaa
//
LOCUS       DSEB01000115            2840 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004649, whole genome shotgun sequence.
ACCESSION   DSEB01000115 DSEB01000000
VERSION     DSEB01000115.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004649"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1732
                     /locus_tag="ENO07_02185"
     CDS             <1..1732
                     /locus_tag="ENO07_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HDS00804.1"
                     /translation="YKDLEDPNHEAMIQQIENNPNITVRKSTKIKRTKGQPGEFDVTL
                     QNGSGEENIRVGAIIQATGWRQYPTKKLEYLGYGKSPNIVTNIEMEEMAKSGKIELPT
                     GGEPKSVAFIQCAGSRDKDYIPYCSAVCCRVSLKQAKYIREQYPDCKIYIIYKDIRSP
                     QQYELFYMQAQADDGIFLTKGEIENVAADNGKIKIDVSETLLGEDIQIETDMVVLASG
                     MVSTNKVGNKFVDIGPMEKELLIGGSEDEKDEDEEPKSEEAKEFLEGGKKAAAGAEAG
                     PEILNLAYRQGTDLPSLKYGFPDSHFICFPYETRRTGIYAAGPVRAPMDIATSMNDAY
                     GAALKAIQSVEAVSVGKAVHPRAGDLSEFDFFMQRCTQCKRCTEECPFGTLDEDEKGT
                     PLLNPNRCRRCGICLGACPERIITFKNYNIVMHSKMIGSISMPDEFDEKPRILVFICE
                     NDAMPALDMVGQMRMKYSPFVRFIPVRCIGSTNVIWLNDALSKGFDGIMLLGCKKGDD
                     YQCHFVKGSQLAATRMTNVQDKLKQLALESERVEIHEIQIDDYDKIPKLIDDFVEEIE
                     TMGYNPFKGM"
     gene            1935..>2840
                     /gene="sat"
                     /locus_tag="ENO07_02190"
     CDS             1935..>2840
                     /gene="sat"
                     /locus_tag="ENO07_02190"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020023502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HDS00805.1"
                     /translation="MADSTVIKPHGADELKILLLEGKEKEEELKKAEKLPKVIMSSRE
                     TGDLIMMGIGGFTPLEGFMGHDDWKGVCQDMKMTEGIFWPIPVTLSYDEKIDPGKEIA
                     LISGETKEIMATMTVTESYKIDKNFECQHVFSTTDDEHPGVQMVNAQKEYNIAGPVKV
                     LSESYFPTDFGEIYMRPSESRRIFEERGWRTVAALQLRNPMHRSHEYLAKIAVEVSDG
                     IFIHQLVGKLKPGDIPADVRVKCINTLVENYFDKSRVVTGGYPLDMRYAGPREGLLHA
                     VFRQNFGCTHMIIGRDHAGVGDYYGP"
BASE COUNT          777 a          659 c          771 g          633 t
ORIGIN      
        1 ctataaggat cttgaagacc caaatcatga ggcaatgatc cagcaaatcg aaaataatcc
       61 caatatcact gtccgcaaat caacaaagat caagagaacc aaaggtcagc cgggtgaatt
      121 tgatgttacc ctgcaaaatg gcagcggtga agaaaacata cgggttggag cgattatcca
      181 ggctaccgga tggcgccagt atcctaccaa gaagctggaa tacctgggat atggcaaatc
      241 cccgaatatc gtgaccaata tcgagatgga agagatggcc aaatcgggca agatcgagct
      301 tcccaccggc ggcgagccta aatctgtggc cttcatccag tgcgccggct cgcgagacaa
      361 ggattatatc ccctattgct ccgcggtctg ctgcagggta tcgctgaaac aggccaagta
      421 tattcgcgag cagtatcccg actgcaagat atatatcatc tacaaggata tccgctcgcc
      481 ccagcagtac gagctctttt atatgcaggc ccaggccgac gacggtattt tcctgaccaa
      541 gggtgaaatc gagaatgttg cagcggataa cggcaaaatt aagatcgatg tgagcgagac
      601 tcttctgggc gaggatatcc agattgaaac tgatatggtg gtactggcct ccggtatggt
      661 ctccaccaat aaggtcggca acaaatttgt cgatatcggc ccgatggaaa aagaactttt
      721 gatcggcgga tctgaggatg agaaggatga ggacgaagag ccgaaatccg aggaagccaa
      781 agaattcctg gaaggcggaa agaaagcggc cgctggagcc gaagcgggac ctgagatact
      841 gaaccttgca tatcgccagg gaaccgatct tccatcactg aaatacggtt tcccggattc
      901 acactttatc tgcttcccct atgagaccag aaggacgggt atctatgccg ccgggccggt
      961 tcgtgctccc atggatatcg ccacctcgat gaatgacgcc tacggcgcgg ctctcaaggc
     1021 tattcagtcg gtagaggctg tttcggtcgg taaagcggtt catcccaggg cgggcgacct
     1081 ctccgagttt gacttcttca tgcagagatg tacacagtgc aagcgctgta ccgaggaatg
     1141 tccctttgga acattggacg aggacgagaa aggaactccg cttctgaatc cgaaccgctg
     1201 caggcgatgc ggtatctgtc tgggcgcatg cccggaaagg atcatcacct tcaagaatta
     1261 caacatagtc atgcactcca agatgattgg ttctatctcg atgccggatg aattcgatga
     1321 gaagccccgg attctggtct ttatctgcga aaacgatgcc atgccggcat tggatatggt
     1381 cggccagatg aggatgaaat acagcccctt tgtgcgcttc ataccggtac gctgtatcgg
     1441 ctcaaccaat gtgatctggc tcaacgacgc tctttccaag ggctttgacg ggatcatgct
     1501 tctgggctgc aagaagggtg atgattacca gtgtcacttt gtcaagggtt cccagcttgc
     1561 tgcaacccgt atgacgaacg tgcaggacaa gctgaaacaa ctggcgctgg aatccgaaag
     1621 agttgagatt catgagattc agatagatga ttatgacaag attcccaaac tgattgatga
     1681 ctttgtggaa gagattgaga cgatgggata caaccccttc aaggggatgt aatcgtctga
     1741 tttgcaggaa gttttgaaaa tataaaaaag acctgattcc aggagaaaag aggtggattt
     1801 cgcttgacaa agcttgtgaa ttatttatct tggcgcagtt tgtgaaaaaa ataatttgcg
     1861 tgccagatcg agcgcctggg cagaacagaa gcaagtaaat ctataaacta taaactaaag
     1921 aacaggaatc agttatggca gattcaacag taataaaacc gcacggtgca gatgagctca
     1981 agatcctgct cctggagggc aaggagaagg aagaggagct gaagaaggct gagaaattgc
     2041 caaaggtaat tatgagctcc cgggaaaccg gcgacctgat tatgatgggc atcggtggtt
     2101 ttacccccct ggaaggattc atgggtcacg acgactggaa aggcgtctgt caggatatga
     2161 agatgaccga gggaattttc tggcccattc cggtgaccct atcatatgac gagaaaattg
     2221 atccgggcaa ggaaatcgca ttgatttcag gcgagaccaa ggaaatcatg gctaccatga
     2281 cggtcaccga atcttacaag atcgacaaga atttcgagtg ccagcatgtc ttctccacca
     2341 ccgatgatga gcatccgggt gtccagatgg tcaatgcgca gaaggaatac aatattgccg
     2401 gtccggtgaa ggttctttcg gaaagctatt tccccaccga ttttggtgaa atctacatgc
     2461 gtccgtcaga gagccgcagg atttttgaag agcgcggatg gaggacagtg gctgccttgc
     2521 agcttcgtaa tcccatgcac cgctcgcatg aatatctggc caagatagcg gttgaagtct
     2581 ctgatggcat cttcattcac cagctggtcg gcaagctgaa acccggcgat attccggccg
     2641 acgtgcgcgt gaaatgcatc aacaccctgg tggaaaacta cttcgacaag agtcgcgtgg
     2701 tcacgggcgg atatcctctg gatatgcgtt atgccgggcc tcgtgaaggg ctcctgcatg
     2761 cggtcttccg gcagaatttc ggctgtacgc atatgatcat aggccgcgat catgccggcg
     2821 tgggcgatta ttatggaccc
//
LOCUS       DSEB01000116            3240 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003484, whole genome shotgun sequence.
ACCESSION   DSEB01000116 DSEB01000000
VERSION     DSEB01000116.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003484"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            15..785
                     /gene="ybgF"
                     /locus_tag="ENO07_02195"
     CDS             15..785
                     /gene="ybgF"
                     /locus_tag="ENO07_02195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HDS00806.1"
                     /translation="MRKFYLALIVLAAGLFLLSGCASRRQMNKMEAQLDYLEKSNANI
                     EQKVNELDSLIKVQEESQRQFLAQLRSSIATFEQRLEQLDYKLVDLNDRIDRLADRPA
                     LITQTTPQQDTSAASDTTAEASTTVDPRVVYNAAYKSLVAGNNEIAILGFEEYLKSFP
                     NTELTDDAQYWLGESYYNMDPPNYEKARVEFQKLVDNYPQSDRMASAKFKLARSLEEM
                     GEQAEAITIYREVVRDYPQTAEANRSRVQLEGLGEAVE"
     gene            789..1286
                     /locus_tag="ENO07_02200"
     CDS             789..1286
                     /locus_tag="ENO07_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00807.1"
                     /translation="MCHIHLAISETKRVPEARAFYDTMEETLNVVIRTSGKFYSYIQG
                     DRYSIDLDRRGNVLGMEIFAPRNEWTVVPDLVPPDKSELRKIRILEHRKEIEPIGFFT
                     NEQGGFCRMRFTEENVSYYFMVAQDLVFEVNVMDELSAVWALNLVQDFGFKKEMKYRK
                     GMNVA"
     gene            complement(1620..2045)
                     /locus_tag="ENO07_02205"
     CDS             complement(1620..2045)
                     /locus_tag="ENO07_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDS00808.1"
                     /translation="MASVNKAILIGNLGRDPEMRHTPSGKPVTTFSIATTEKWRDQNG
                     EMQESTEWHNIVLWGRQAEIAKEYLSKGRPVYIEGRIQTRSYEDKNGVKKYITEIVGR
                     HMQFLGARGESTGDYTPPPPPKDEEAPFDNTVEDDDLPF"
     gene            2177..2851
                     /gene="rsmI"
                     /locus_tag="ENO07_02210"
     CDS             2177..2851
                     /gene="rsmI"
                     /locus_tag="ENO07_02210"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDS00809.1"
                     /translation="MYGKLYLVATPIGNLGDITFRAVETLKTVDLIACEDKRVSGKLL
                     DHYGIKGKLMTYNDPNKFKSAPLIIERLRGGEDAALITDAGSPGISDPGFHLVRSAID
                     EGIEVQAVPGPSALIYALTVSGLPLHNFVFEGFLPPKGSKRNKRMDILVEEKRTIVLY
                     ESPHRLLSTLKSLYEKLGNRRAVIARELTKMHEEILRSDLEKLVAKYETEKPRGEFVL
                     IVEGKN"
     gene            2928..>3240
                     /locus_tag="ENO07_02215"
     CDS             2928..>3240
                     /locus_tag="ENO07_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00810.1"
                     /translation="MLIFRQNVPNWELHFIFNASVAVFVLHISRMPVNPEGILQKTVR
                     RWYFLVLLLFIYQQTGGLVFLYIPEWLDNQLAQLEFNIFGLHPTIYLEKFNIPILNEY
                     FM"
BASE COUNT          823 a          746 c          812 g          859 t
ORIGIN      
        1 agaatagggt aatcatgcgt aaattttatc tggccctgat agtcctggct gccggattat
       61 ttcttttgag cggatgcgcc tcgcgccgtc aaatgaataa gatggaagcc cagctggatt
      121 atctggaaaa atcgaatgca aatatcgaac agaaggttaa cgagctcgac agcctgatca
      181 aggttcagga ggaatcccag cgtcaattcc tggcccagct caggtcatcc attgcgacct
      241 ttgaacagcg cctggaacaa ctggattaca agctggtcga tctgaacgac cggatagaca
      301 ggctggccga ccgtcccgct ctgataacac agacaacgcc gcagcaggat acatcggctg
      361 catcggatac gactgcggaa gcttcgacga cagttgatcc cagggtggtt tacaatgccg
      421 cttacaagag cctcgtggcc ggaaataatg aaatcgccat cctgggcttc gaggaatatc
      481 tgaagagttt tcccaatacg gaattgaccg atgatgccca gtactggctg ggggaaagct
      541 actataatat ggatccgccc aattatgaga aggctcgggt ggaattccag aagctggtcg
      601 ataattatcc ccagagcgac cgaatggcca gcgccaagtt caagctggcg cgcagtctgg
      661 aggagatggg tgagcaggct gaggcgatca ctatttatcg tgaggtggtg agagattatc
      721 ctcagaccgc ggaggccaac cgctcccggg tacagctgga aggcctcggg gaggctgtgg
      781 agtaagttat gtgtcatatc catctggcga tcagcgaaac caaaagagtt ccggaagcca
      841 gagcgtttta tgacacaatg gaagagacgc tgaatgtcgt tatacgcacc agcggtaaat
      901 tctattcata tatccagggt gacaggtatt ccatcgatct cgaccgccgc ggcaatgtgc
      961 tcgggatgga gatcttcgct ccccgcaatg aatggactgt agtgcctgat cttgtgcccc
     1021 cggacaaaag cgaattgcgc aagattcgta ttctggagca tcgcaaggaa atcgaaccga
     1081 tcggcttttt tacgaatgaa cagggcggat tctgccgcat gcgcttcacc gaagagaatg
     1141 tcagctacta tttcatggtg gctcaggacc tggtctttga ggttaatgtt atggatgagc
     1201 tctctgcggt ctgggcattg aatctggtcc aggatttcgg cttcaaaaaa gaaatgaaat
     1261 atcgtaaagg catgaatgta gcctgaaata tatcgtcatg ataacggaag gagaatgaca
     1321 cggtcatcct cctttttata tgcttataac agtgtattcc acccgacggg cctgttgaaa
     1381 aaccccatta ttgagtcggt catcctgagt cccgatttat cgggacatcg atacccacat
     1441 gtaatgtggg tcaaaatgat tatcctgcta tgttaatttc taaattggac gttttgtcta
     1501 aaccgaatgt caaatgtctt tgagatttga caatttttgt ttttaaagat tgtcaagccc
     1561 ctccaaaacc atttggcttt tccgaggctt gacctttgcc aatgtcccaa gagttttcac
     1621 taaaacggca gatcgtcatc ctcgaccgta ttatcaaagg gcgcctcctc atccttgggc
     1681 ggcgggggtg gggtgtaatc accggtggat tcaccgcgtg cgccaaggaa ctgcatatgt
     1741 cttcccacaa tttcggtaat gtatttcttc acgccgtttt tatcctcata actgcgggtt
     1801 tgaatccgtc cctcgatata gaccggcctt cctttgctca aatattcctt ggcaatttct
     1861 gcctgcctgc cccataagac aatattgtgc cattccgtgg attcctgcat ctcgccgttt
     1921 tgatcccgcc acttctctgt ggttgcgatc gaaaacgttg tcaccggctt gcccgaggga
     1981 gtgtggcgca tctcgggatc tcttcccagg ttgccgataa ggatcgcttt gtttacagaa
     2041 gccatattct ctccttttct tgatttgcgc caatataaag gtatgaaaaa atcatgtcaa
     2101 tcatgtcttt attaaaatct gctgttggtg tcatatttat ttgacaggcg gggtaaaaac
     2161 aaataatatc agcctgatgt acggaaaatt atacctggtt gcgaccccga tcggtaatct
     2221 gggggacata acttttcgag ccgtggaaac attaaaaacc gtggacctta tcgcctgcga
     2281 agataaaagg gtctctggta agctcctgga tcattatgga attaaaggca agcttatgac
     2341 ctataacgat cccaataaat tcaaatctgc cccgctgatt atcgagcggc tcaggggagg
     2401 cgaggatgcg gccctgataa ctgatgccgg ttctccggga atttcggatc cgggatttca
     2461 cctggtccga agcgccatcg acgagggcat agaagttcaa gccgttccgg ggccctcggc
     2521 attaatatat gccctgactg tgtccgggct gccgctgcat aattttgttt ttgaaggatt
     2581 tttgcccccc aaaggatcga agcgcaacaa aaggatggat attcttgtag aagagaagcg
     2641 cacaattgtt ttatacgagt ctccccacag gcttctgagt acgttgaaaa gcctgtatga
     2701 aaagctgggc aatcgcaggg cagtgatcgc gcgggagctg actaaaatgc atgaggaaat
     2761 attgcgctct gatcttgaaa aactggttgc caaatatgaa accgaaaagc cccgagggga
     2821 atttgtcttg atagtggagg ggaaaaatta gcacacattc aaacagattc aacttcctgc
     2881 cggttgatta ctttgttctg ctttacaacg tggtagtggg attatcggtg ctgatttttc
     2941 ggcagaatgt ccccaactgg gaattgcatt ttatctttaa tgcctctgtt gctgtcttcg
     3001 ttctgcatat cagccgtatg cctgtaaatc cggaagggat tttgcagaaa acagtgcgcc
     3061 gctggtattt tctggtcctg ctgctgttta tctaccagca gaccgggggg ctggtatttt
     3121 tgtacatccc ggagtggctg gataatcagc ttgctcagtt agaattcaac atcttcggac
     3181 tgcacccgac catttatctg gaaaaattca atatcccgat actgaacgaa tattttatgc
//
LOCUS       DSEB01000117            1649 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1015484, whole genome shotgun sequence.
ACCESSION   DSEB01000117 DSEB01000000
VERSION     DSEB01000117.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1015484"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(665..1384)
                     /locus_tag="ENO07_02220"
     CDS             complement(665..1384)
                     /locus_tag="ENO07_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00811.1"
                     /translation="MKAFIILFVALCLILTFIFCEENNTASADQVPGRTETLLGLDAN
                     HANLYQQFDSIYTYTPDLRIDVDTSLVTLITEDISGDRSAFDIGFPSGKLARLIITTN
                     SVAITGFYQKIDGRDSLFYFVDIPEIFPDQLRTEDSWSFYVPPLFRDNQAVITFYLNY
                     GFGYEVTRTYVGTERIVVPAGAYTAHVIQSEYRLQGISEVIKTDTEYLVAGIGLVRMQ
                     SEGKFGKSHIHLIESQPLAEE"
     gene            1422..1634
                     /locus_tag="ENO07_02225"
     CDS             1422..1634
                     /locus_tag="ENO07_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00812.1"
                     /translation="MEVPVNKNNVHPGYMQPAKLNFLLALVLTLCGVLAGYFATVYDI
                     KMNLAHKAENEALVSLEKRVLSLEYT"
BASE COUNT          435 a          366 c          460 g          388 t
ORIGIN      
        1 gtcaaggtat ggttctactg gctctgggaa tcgcagggaa gcaaagcgct tctgtcgaat
       61 atcgacgggg ggatttataa ggaaggttcg atcggattta ttttcagctt tccggagtgc
      121 agtatctgcg gggaggatat tcgtaattgc gcacacacgc ccttgaaaag ttatccctcg
      181 gatcagggtg aggagatatg tcattacagc tatcgtaaga tcgagaaagt gctggagaca
      241 tccctggtat tccggggagc gactgccgac accaggacaa ctaacgagct taccggcaat
      301 tcagggaata cccggaaaca gggcggttac atgctcaaga ggatcaagga ttcagccata
      361 atcgaattcg agcacaagga catacccttc acagtgattt ttccgcagtt taacatcgct
      421 tctttgaagc gcaagggctc gctggtcggt tatcctgttt ccgatgaacg gcagagaagg
      481 ctcgatctca gattggataa gctgaaaggc aggcgggaga aaggcgagct ggaaatcgag
      541 ggaaaacgca gaggcggatt tcttctcagg ctgaatggac ggacattaga gggaagatat
      601 ttgatccgcc cggcattctg ggataagagg agattggtac tttttacaag gctcagctaa
      661 atcgttactc ttcggccagc ggctgagatt ctatcagatg gatatgggac tttccaaatt
      721 taccctcgga ctgcatgcgc accaggccga ttccggcaac cagatattcg gtgtcggttt
      781 tgatcacttc gcttatgccc tgcaggcggt attcggactg gatgacatgg gctgtgtaag
      841 ctcccgcggg taccacaatt ctttcggtgc cgacatacgt gcgtgtgacc tcgtatccaa
      901 agccgtaatt caggtagaaa gtgatcaccg cctggttgtc gcggaaaagc ggggggacat
      961 aaaaggacca gctgtcctcg gtgcgcaact ggtcgggaaa tatctcgggt atatccacga
     1021 aatagaagag agaatcccgg ccgtcgattt tctgatagaa cccggtaatc gctacagagt
     1081 tggtggtaat aattaagcgg gccagttttc cagaggggaa accgatatcg aaagcgctcc
     1141 ggtcgccgga aatatcttca gtgatcaagg tcaccaggct ggtatcgaca tcgatcctga
     1201 ggtccggggt gtaggtgtaa atgctgtcga actgctgata cagattggca tggttggcat
     1261 caaggcccag gagcgtttca gtccttccgg gcacctgatc ggccgaggcg gtattgtttt
     1321 cctcgcaaaa aatgaatgtc agtataagac atagtgctac aaataagatt ataaacgcct
     1381 tcataaacac aacctctctt tctaagggag aaaataaaac tatggaagtg cccgtcaaca
     1441 aaaacaatgt tcatccgggt tacatgcagc ccgccaaatt gaatttcctg ctggcgctgg
     1501 tgctgacttt atgcggggtg ctggccggat attttgcgac agtttacgac atcaagatga
     1561 acctcgctca taaggccgaa aatgaggccc tggtcagcct tgaaaagcgg gtcctgagcc
     1621 tggaatatac ctgaaggaga ggatgataa
//
LOCUS       DSEB01000118            1564 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1017472, whole genome shotgun sequence.
ACCESSION   DSEB01000118 DSEB01000000
VERSION     DSEB01000118.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1017472"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..945
                     /locus_tag="ENO07_02230"
     CDS             <1..945
                     /locus_tag="ENO07_02230"
                     /inference="COORDINATES: protein
                     motif:HMM:PF13414.4,HMM:PF14559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDS00813.1"
                     /translation="NFSSALTNLGNVLRIKGNVDEAVQMYQKALTVDPTLFEARVNLI
                     SLAVATGRTSSFNLHLEKLQEYYPESEYTSYFLGEQKKNQGECEEAVQLYEDFLKKNP
                     DHLNTKLSLIDCNLEVGNYDRAMQILKELALRMGDNPSLKERALGPAEKAFQAGDYEK
                     AKQYYESMSAIWPDDPKFRFGAASAMINLDELAEARVILEDMVHNYPESSEILSNLGL
                     VYAKMGKPEWAREQFEAAIAIDSSAVAYYNLGKIFEEQGDTARANSCYILASLKEPEM
                     FGLQDYMMEVSMEKAERIARGDTAGMIFPEYARDSTNR"
     gene            920..>1564
                     /locus_tag="ENO07_02235"
     CDS             920..>1564
                     /locus_tag="ENO07_02235"
                     /inference="COORDINATES: protein motif:HMM:PF01938.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDS00814.1"
                     /translation="MPETLQIGDRLDVAIERLSFTGKGIGRVNNFVIFIPHTIPGDVV
                     KAEITSRKKRYADARAVDFVSRSSDSIEPRCRHFGICGGCSFQNLPYELQLEAKQDAV
                     QEHIRRIGKIEQPPIDKIIPSDKQLFYRNKMEFSFKPDQEDFLKLGLHYRGEWEKIFD
                     VEECLLQSEISNRIVNRVRDFFKAKRIPAYHLSEHHGHLRFLIIRESLATGEVML"
BASE COUNT          413 a          366 c          430 g          355 t
ORIGIN      
        1 aatttcagct ccgccctgac caacctgggc aatgtgctgc ggattaaggg aaatgtggat
       61 gaggctgtgc agatgtatca gaaggcattg acggtcgatc ccacactttt cgaagcgcgg
      121 gtcaacctga tcagcctggc agttgccacg ggccgaacct cctcgtttaa tctgcacctg
      181 gaaaagctcc aggagtacta cccggaatcg gagtacacaa gctacttcct gggtgagcag
      241 aagaaaaacc agggtgaatg tgaggaggcc gtgcagttgt atgaggattt tctcaagaaa
      301 aatccggatc atttgaatac caaactctct cttatcgact gcaatctgga ggtgggcaat
      361 tatgatcggg ctatgcagat tctgaaggaa ctggccctgc gcatgggaga caatccgagt
      421 ctcaaggaaa gagctctggg tccggcggag aaagccttcc aggcgggaga ctatgagaaa
      481 gccaagcagt actacgagag catgtcggca atctggccgg acgatcccaa attcaggttc
      541 ggtgcggcct cggccatgat caatcttgat gagctggccg aggcgcgggt gattctcgag
      601 gacatggtgc acaattaccc ggaatcctct gaaattcttt ccaacctggg gctggtttat
      661 gccaagatgg gcaaaccgga atgggcccgg gagcagtttg aggcggcaat tgcgatcgac
      721 tcttctgcag ttgcatacta caatcttgga aagatttttg aagaacaggg ggacactgcc
      781 agggccaatt catgctatat tctcgcctcg cttaaagagc cggagatgtt cggtcttcaa
      841 gattatatga tggaagtttc aatggaaaag gccgagcgta ttgcccgcgg cgatactgcc
      901 ggaatgatat tccccgaata tgccagagac tctacaaata ggtgatcgcc tggatgtggc
      961 catcgagcgc ctctccttta ccggaaaagg tattggaagg gtcaataact tcgtgatctt
     1021 catcccccac actattccag gcgatgtggt taaggctgaa atcacctccc gcaagaaacg
     1081 atacgcggat gccagggcag tcgattttgt cagtcgttca tccgattcaa tcgagcccag
     1141 gtgccgacat ttcgggatct gcggcgggtg cagtttccag aatctgccct atgaactgca
     1201 gctggaggca aaacaggatg cggtacagga acatatccgc agaatcggta aaatcgaaca
     1261 gccccccatt gataagataa taccctcgga taagcaatta ttctatcgca acaagatgga
     1321 attctctttc aagcccgacc aggaggattt tctcaagctg ggactgcatt accgcggcga
     1381 atgggagaag atttttgatg tggaggaatg ccttctgcaa tcggagatat caaacagaat
     1441 tgtgaatcgt gtcagggatt ttttcaaggc gaagaggata ccggcctatc atctatcgga
     1501 acatcacggg catctgagat ttctgataat aagggaaagc ctggccacag gcgaggtcat
     1561 gctg
//
LOCUS       DSEB01000119            1835 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1012274, whole genome shotgun sequence.
ACCESSION   DSEB01000119 DSEB01000000
VERSION     DSEB01000119.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1012274"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..666)
                     /locus_tag="ENO07_02240"
     CDS             complement(<1..666)
                     /locus_tag="ENO07_02240"
                     /inference="COORDINATES: protein motif:HMM:PF01139.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HDS00815.1"
                     /translation="MQSHKVSNGIWQIDPDQKQGMRVPARIYASEKLIGSMDEGVYEQ
                     ITNVACLPGIVSRAMVMPDGHWGYGFPIGGVAAFSTSDGVISPGGIGFDINCGIRMLR
                     TNLTLEEVKPKMKELVNMLFKVIPTGVGGKGLVRLDRKKFEDVMRKGVKWCLENGYAT
                     EADYDSIEENGHLKWADPSTVSDRAVQRGINQLGTLGSGNHFLEIEVVPEDLVYDKEL
                     APKF"
     gene            complement(677..1102)
                     /locus_tag="ENO07_02245"
     CDS             complement(677..1102)
                     /locus_tag="ENO07_02245"
                     /inference="COORDINATES: protein motif:HMM:PF01951.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HDS00816.1"
                     /translation="MKKYEYDDSVAIGDCAYHAFGQTLEELFVNAGLAATECMVDPDT
                     VDADQEQEIELSADSESNLLYEWLEEIVYLKDAEDIFLTDFQIEFLDGNKKLRAKARG
                     EKINPQKHGIKADVKAVTMYRFDLKKTEEGWEAFIVLDL"
     gene            complement(1089..1313)
                     /locus_tag="ENO07_02250"
     CDS             complement(1089..1313)
                     /locus_tag="ENO07_02250"
                     /inference="COORDINATES: protein motif:HMM:PF06305.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HDS00817.1"
                     /translation="MNGKTILVIILAIILVLFLILNGARTEVNFIFFQVRTSTAILIF
                     ISAIIGFLIGVALPYAMKSKSKAKGKSEKI"
     gene            complement(1414..1602)
                     /locus_tag="ENO07_02255"
     CDS             complement(1414..1602)
                     /locus_tag="ENO07_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDS00818.1"
                     /translation="MAMKITDECTACALCEPECPQGAIEEGDPIYIIDPDLCNECEDV
                     EGGSQCVLVCPVDCIEKA"
BASE COUNT          437 a          478 c          395 g          525 t
ORIGIN      
        1 gaatttggga gccagctcct tgtcgtagac caaatcttca gggaccacct ctatttccag
       61 aaaatggttg ccggaaccaa gcgtgccgag ctgattaatt cctctctgaa cggcgcgatc
      121 cgataccgtg gatggatcgg cccacttcag gtgaccgttc tcctcaatgg aatcgtaatc
      181 agcctcggtg gcatagccgt tctccaggca ccacttaacg cccttgcgca tgacatcctc
      241 aaatttcttg cggtcaagcc tgaccagtcc tttgcctccg actccagtgg gaatgacctt
      301 gaagagcata ttgaccagct ctttcatctt cggctttacc tcttccaggg tcaggttcgt
      361 gcgcagcatt cgtatgccgc aattgatatc gaagccgatt ccgccgggag agataactcc
      421 atcggaagtg gagaaggcgg ccacgcctcc gattgggaag ccgtagcccc agtgaccatc
      481 gggcatgacc atggcgcggc tgactattcc gggcaggcag gcaacgttgg ttatctgctc
      541 atatactccc tcgtccatgg agccgatcaa tttctcggat gcgtaaatcc tggcggggac
      601 acgcatgccc tgtttttgat cggggtctat ctgccagatg ccgtttgaga ctttatgtga
      661 ttgcattttt atcgcctcac aaatcgagaa ctataaaggc ttcccagcct tcttctgttt
      721 ttttcagatc gaatctatac attgtaacag ctttaacatc cgctttgatt ccgtgttttt
      781 gaggattaat cttttctcct ctcgcttttg cccttaattt tttattgccg tccagaaatt
      841 caatctggaa atccgttaag aatatgtcct ccgcatcctt caggtataca atctcctcca
      901 gccattcata gagcagattg gattcagaat cggcagatag ttcaatctcc tgctcctgat
      961 cggcatccac ggtatcagga tcaaccatgc actctgtcgc cgccagccct gcgttcacaa
     1021 aaagctcctc caaagtctgt ccgaaagcgt gataggcgca gtcgcctatg gcaacgctat
     1081 cgtcatactc atattttttc acttttcccc tttgctttgg atttactttt catcgcatac
     1141 ggcagagcca cgccaatcaa aaagccgatt atagcagata taaaaatcag gatggcggtt
     1201 gatgttcgta cctggaaaaa gatgaaattg acttcggtgc gggcgccatt aagtatcaga
     1261 aacagcacca gaattattgc cagaattatt accaggatcg tctttccgtt catagattcc
     1321 tccttttttt ataaacatat aagagattcc gctttgtcgc aataaaaaaa acaggcctag
     1381 acggcctgct cctaaactga gagtcaaatc tatctatgcc ttttcgatac agtcaaccgg
     1441 acaaaccaac acacactgtg agccaccttc aacatcctcg cattcattgc agagatccgg
     1501 atcaatgata taaatcggat cgccctcttc gattgctccc tggggacatt ccggctcaca
     1561 tagagcgcag gcggtacatt catcggtaat tttcattgcc attctcggga tcctcctgtt
     1621 caggtataag gcaatatagt atactaatat tgtattcttt attatacggt ttgattttcc
     1681 gtttattggg aattatcggg atccgcaggc tgctcagttt ttttcggttt ttctgcttcc
     1741 ggctcgactt tttcgaccgc gaccagttca tatcggaggt tttgcttcgg cagaacgacc
     1801 agagccacgt gatgatccgg ggtgatgtaa taata
//
LOCUS       DSEB01000120            2236 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1007906, whole genome shotgun sequence.
ACCESSION   DSEB01000120 DSEB01000000
VERSION     DSEB01000120.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1007906"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(207..1193)
                     /locus_tag="ENO07_02260"
     CDS             complement(207..1193)
                     /locus_tag="ENO07_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDS00819.1"
                     /translation="MSRPLRIEYSGAWYHVMNRGRRGEDIFANSEDFETFLKLLREGA
                     ELWNVKIGAYCLMSNHYHLLVQTPQGNLSRFMRHLNGIYTQRYNRSHRCDGQLFRGRY
                     KAILVEEDRYLLELVRYIHRNPLRAGMVEDLDQYAWSSHPGYLSSGKQWDWLHKEFFL
                     AILATDPRKRFLAYQQFIQQEDSEEICQVFDKKKLPSILGSDEFVDRVKNRFFAEKIH
                     SQVPEAGQLAPTMCRIIEAVCQYYGSDINDILQSKRGVSNEPRSVAIYLARVLRRDSL
                     LAISSEFGMQSYSPASSAIERVIKRLSTDQGFQERVAEIKQVVAGEKCQAET"
     gene            complement(1462..1968)
                     /locus_tag="ENO07_02265"
     CDS             complement(1462..1968)
                     /locus_tag="ENO07_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00820.1"
                     /translation="MPTVIVQVNDLMQTNYNYERIEPVGKNYHPDFRPELKPEEMLAL
                     GVFGGKYMTDCKDEFPDEWFKNAKLCSERHDPDLNFFGVNASQPLSVWQKKGWIYEED
                     PRGWFQWYCRYYMGRRCRDDERQIKRWIGMRRHITQIKRNCFEADLNCRRKQRQALLH
                     WAYDSRKY"
     gene            complement(2031..>2236)
                     /locus_tag="ENO07_02270"
     CDS             complement(2031..>2236)
                     /locus_tag="ENO07_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013388686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00821.1"
                     /translation="APLAAGIVERRGGMLIHGAIIAREYGLPCVTGVPDATELIRTGD
                     LVTVDGYLGLITIGTEAGEGQEC"
BASE COUNT          558 a          594 c          514 g          570 t
ORIGIN      
        1 gcaaccatca acactctttg ctggtctggc ataaagcttt cgttcaggag gcaggacttt
       61 tagcccaaac aagaaagatt gggcgagtcc aattatagcg cagacccact ggatgggaag
      121 gaaaagaatc ggaccattgg cttggttcga tgtcaatcgg cattcagcag tgtccggtta
      181 gggacttgca tgtgatacca aaggggtcaa gtctccgctt gacacttctc cccggcaacg
      241 acctgtttta tctctgcaac gcgctcctga aagccttggt cagttgacag tcttttgatg
      301 acccgctcta tggcgctgct tgcagggcta taactctgca ttccaaattc tgagcttatt
      361 gccagtaaac tatcccgacg taaaacacga gccagataga tcgccacact tctcggctca
      421 ttgctcacac cacgcttgga ctgcagaata tcgttaatat cactcccgta gtattgacaa
      481 accgcttcta ttattctgca catggttggt gccagttgcc ctgcctccgg cacctggctg
      541 tggattttct ctgcaaaaaa gcgattcttg acccggtcga caaattcatc tgaccctaaa
      601 atgctcggca acttcttttt gtcaaaaacc tggcagattt cctcagaatc ctcttgttga
      661 ataaattgct gataagccag gaatctcttt ctgggatctg tcgcaagtat ggccaggaag
      721 aattctttgt gaagccagtc ccattgtttt ccagaagaca aatagccggg atgactgctc
      781 caggcatact ggtcaaggtc ctcgaccata ccagcccgca acggattcct gtgaatatag
      841 cggacaagtt ccagcagata gcgatcctct tcaacgagga tcgccttgta tcgaccacgg
      901 aacaattgcc cgtcgcaacg atgagaccgg ttataacgtt gggtgtagat tccattcagg
      961 tgtcgcataa agcgtgacag gttgccttgc ggcgtttgca cgagcaagtg gtaatgattc
     1021 gacatcaggc aataggcacc gatcttgaca ttccaaagtt cggcgccttc acgcagaagt
     1081 ttgagaaaag tttcaaaatc ttcgctgttt gcaaaaatgt cttcaccacg gcggccacga
     1141 ttcattacgt ggtaccaggc tccggaatat tcaatgcgca atggacggct catgggggga
     1201 agactaacaa aaggatgtac atgtgtcaaa cggagacttg accccatttt cagtcttcac
     1261 aacctcatag tctggttctt cgtaagccaa tgcaatgtct ccctgcaaaa gagatatccc
     1321 tagcaccagt gtgcacacta ttgttcgtat caaatagctc aacactacag tcatttacat
     1381 cgctccttta agaatcaaga tcaaggtcag gactaccgcc acccggcttt ggcgtaacga
     1441 tatccttatg aggcgcgcga ctcaatactt tcgcgagtca tatgcccagt gaagaagggc
     1501 ctgcctctgc ttgcggcgac aattcaagtc ggcctcgaag cagtttcttt tgatctgggt
     1561 gatgtgtctg cgcatgccta tccatctctt gatctgacgt tcatcatctc tacagcgtcg
     1621 ccccatgtag tatcggcaat accactggaa ccatccacgt ggatcttcct catagatcca
     1681 ccctttcttt tgccacacag ataagggttg tgacgcattc actccaaaaa aattcagatc
     1741 cgggtcatgc cgttcggaac atagcttggc atttttgaac cattcgtctg ggaattcatc
     1801 cttgcaatct gtcatgtatt taccgccgaa cacacccaac gcaagcatct cttcaggttt
     1861 cagctcggga cgaaaatctg gatggtaatt cttgccgacc ggttcgatgc gttcataatt
     1921 gtaatttgtc tgcatcaaat cattaacctg aacgataacg gtgggcataa aaacctccct
     1981 taataaccta ggatgcgctg ggaaaagtct tgggacaacc aggctttcgg tcagcattct
     2041 tgaccctccc cggcttcagt tccgatagtg atcagtccca ggtatccatc aaccgtcaca
     2101 aggtcgccgg tacggatcag ttcggtggca tcaggcactc cggtgacgca ggggagaccg
     2161 tactcccggg cgatgatggc gccgtgaatc agcatgccgc cgcgccgctc gacgatacca
     2221 gcggccaatg gcgcca
//
LOCUS       DSEB01000121            1838 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1012236, whole genome shotgun sequence.
ACCESSION   DSEB01000121 DSEB01000000
VERSION     DSEB01000121.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1012236"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..568)
                     /locus_tag="ENO07_02275"
     CDS             complement(<1..568)
                     /locus_tag="ENO07_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00822.1"
                     /translation="MLFNFSEHKRDHTCGELRSSDAGSTVTLNGWVHRRRDHGGLIFI
                     DLRDRYGITQLVFRPEKLSEQQMKEAGKLGYEFVISATGTVNKRPEGMINPEMDTGEV
                     EIEVEKLIILNPSKTPPFEIEDEVKALEDLRLTYRYLDLRRTPLQRNIAIRHRFIKAV
                     RDFMDSEGFFEIETPMLMKSTPEGARDYI"
     gene            complement(653..1084)
                     /locus_tag="ENO07_02280"
     CDS             complement(653..1084)
                     /locus_tag="ENO07_02280"
                     /inference="COORDINATES: protein motif:HMM:PF04892.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teicoplanin resistance protein VanZ"
                     /protein_id="HDS00823.1"
                     /translation="MADKSEFWSNEISNGFKRSIFLHLPALLYMALIFYISSLRSISP
                     PSIGLGIEDKIYHFGEYALLSLFLYVALLSYRHKSIQSSIHIIAVVIACIFAGTDELH
                     QYFVPGRESTVGDLIADCLGAITAQAVIWYYLKLRRQKSID"
     gene            complement(1104..>1838)
                     /gene="rlmD"
                     /locus_tag="ENO07_02285"
     CDS             complement(1104..>1838)
                     /gene="rlmD"
                     /locus_tag="ENO07_02285"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDS00824.1"
                     /translation="VMLILVTNRGDYEYLDDFVEMVTSEFPRVTSVVHVINSKKANIA
                     VGESEKIIRGNEHITEYIGGNKFLIRPSSFFQTNSRQTEILYNRAAEMGELGSDEKVL
                     DLYTGCGTIAIHIARNVGSVLGIELNPDAVVSANENARLNEINNCEFVAGDVRKTMDK
                     LIDEGRKFDTIIADPPRAGIGHKSLVRIFQLQPEKLIYISCNPATLAADIRELRENGY
                     RLDRTVPVDMFPHTFHIETVSRITRK"
BASE COUNT          460 a          475 c          403 g          500 t
ORIGIN      
        1 caatatagtc acgcgcgccc tcgggagttg atttcatcag catgggtgtt tcaatctcaa
       61 aaaatccctc gctgtccata aaatctctga ccgctttaat gaatctgtgc ctgattgcga
      121 tattcctttg cagcggagtt ctgcgaagat ccagatagcg ataagttaag cgaaggtcct
      181 caagagcctt aacctcatcc tctatttcaa aaggcggtgt tttggaggga ttcagtatta
      241 tcagcttctc aacctctatt tccacctcgc ccgtgtccat ttcaggattt atcatcccct
      301 ccgggcgctt gttaaccgtt ccggtggctg aaattacaaa ttcataaccc agctttcccg
      361 cttccttcat ctgctgctcg gaaagcttct cgggacgaaa aacaagctgg gtgatgccat
      421 aacgatcgcg caggtcgata aatatcagtc cgccatgatc gcggcgtctg tgaacccatc
      481 cattaagagt aaccgtagaa ccggcatcag agctcctcaa ttctccgcag gtgtgatctc
      541 ttttgtgctc ggaaaaatta aacaacattc tctccaactt tatgacacac tttaaactat
      601 tatcggtctg attttcacaa gttcaagcct ataagatagg tgggggaatg tattagtcaa
      661 tgcttttttg gcgcctgagc ttgaggtaat accagatcac agcctgggcc gtaatcgccc
      721 ccaggcagtc tgcaatcaga tcccccactg tggattcccg cccgggtacg aaatactggt
      781 gcagctcgtc cgtgcccgcg aagatgcagg caatcaccac tgcgattata tgtatgctgc
      841 tttgaattga cttatgccga taggacaaaa gtgctacata taaaaacaat gaaagcaggg
      901 cgtattcacc gaaatgataa attttatctt cgatacctaa accgatcgac ggcggcgata
      961 tacttcgcag gctggagata taaaatatca gcgccatata caacagggcc ggcaggtgca
     1021 gaaatatgga acgcttgaaa ccgtttgaaa tttcatttga ccagaactca cttttgtctg
     1081 ccatgaatac ctgtcaatac tctctatttc cgggttatcc gtgaaaccgt ttcaatgtgg
     1141 aaggtgtgcg ggaacatatc gaccgggacc gtcctgtcca gcctgtagcc attttctctc
     1201 agttcccgta tatctgccgc cagtgtggcc ggattgcagg aaatgtagat taatttttcc
     1261 ggctgaagtt ggaatattct gaccagagac ttatgcccga taccggcgcg gggcggatcg
     1321 gcaattatgg tatcgaattt tcgtccctcg tcgatcagct tatccatggt ctttcttaca
     1381 tctcccgcaa cgaactcgca attattaatt tcattaagcc gcgcattctc attggcgctt
     1441 actaccgcat ccggattgag ctcaatcccc agcaccgagc cgacattacg ggcgatatga
     1501 atcgcgatgg tcccgcatcc tgtatacagg tccagcactt tttcgtccga ccccaattca
     1561 cccatctcgg ccgcccggtt atacaggatt tcggtctggc ggctgttggt ctggaaaaat
     1621 gaagaagggc ggatcaggaa cttgttgccc ccgatatatt ctgtgatatg ctcgttgccc
     1681 cggataatct tctccgattc tcccacggca atattggctt tcttcgaatt tataacatgc
     1741 actactgaag tcacccgggg gaattcggag gtaaccattt ccacgaagtc atccagatat
     1801 tcataatcac cccggttagt aactaatatc agcatgac
//
LOCUS       DSEB01000122           15623 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000109, whole genome shotgun sequence.
ACCESSION   DSEB01000122 DSEB01000000
VERSION     DSEB01000122.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15623)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15623)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15623
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000109"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            151..1119
                     /locus_tag="ENO07_02290"
     CDS             151..1119
                     /locus_tag="ENO07_02290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDS00825.1"
                     /translation="MITKGVKIRIKELWERFKSHFRPKRIKDFWAHYGLGLIDRIGKN
                     HVFLEASGLAFSIIVCIVPFVLIVFAVLGFVLESPTIRVEINNFIDRLIPYSDAAETV
                     KQIVFERAQEFKVNKNIAGIFGILGLLWASSRLFSSMRTILNISYKNRLGGSIFGDVV
                     AKLRDLAMVLLVLFYFLLATTVIPGLAVLEKVTEDSTILEFLHIQFVGDIIVQAASFI
                     ILTFAFFVIYYFVPQAKIPLKVILVSALWGAFFWGIAEYLFRIYINNFVNLKRIYGAY
                     VLFIVAVFWIFYTSVVLIVGAEIGQLYRERLRKREKEARKEQVAET"
     gene            1223..1450
                     /locus_tag="ENO07_02295"
     CDS             1223..1450
                     /locus_tag="ENO07_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDS00826.1"
                     /translation="MKALDVIFLILLVVGGLNWGLVGIFNFNLVAAIFGEMSVISRII
                     YILVGIAALYNLVLWKSMQQRWGRGHAPATA"
     gene            1690..2658
                     /locus_tag="ENO07_02300"
     CDS             1690..2658
                     /locus_tag="ENO07_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1722 domain-containing protein"
                     /protein_id="HDS00827.1"
                     /translation="MPDVLQTEKAIKVGISACLLGQMVRYDAGHKRDRYSTDVLSNYF
                     QFVSVCPEVEVGMGVPREAVRLVGTAEETRMVGNKTGKDWTEKVVAFNAKRANELEKE
                     NLSGFILKKDSPSCGMERVKVYNEKGMAEKNAVGMWPRALKKQFPIMPMEEEGRLNDP
                     RLRENFIVRVFAYHRLQNLFDDRYSRGKMVRFHTAHKLLLMSHSPKHYKELGKLVAEV
                     KKYSPSEMREKYSAVFMEGLGYKTTAKKNVNVLHHILGYLKTNLTSSEKKDILQVIDD
                     YHKELTPLVVPLTLLRHYVTIHDIEYIKDQIYLTPHPKELMLRNHV"
     gene            complement(2667..3050)
                     /locus_tag="ENO07_02305"
     CDS             complement(2667..3050)
                     /locus_tag="ENO07_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013414090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDS00828.1"
                     /translation="MIVATDLIIQYPDGKIILIKRKNDPYQGYWALPGGMVEENETVE
                     EAAVREAREETGVRVELKRLVGVYSDPDRDPRGRVMSVTYLARPVSGEPKAADDAAEL
                     ILTEDFFAMKLAFDHEQMLKDAFGH"
     gene            complement(3071..4417)
                     /locus_tag="ENO07_02310"
     CDS             complement(3071..4417)
                     /locus_tag="ENO07_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDS00829.1"
                     /translation="MQYLDSILSRAVDIAWGIPLVVLLITAGVYFTIVGRFVPFRHLK
                     HAVNILRGKYDNPDDPGEINHFQALSSALSATIGMGNISGVAIGITIGGPGAIFWMWV
                     AGIVGMSTKYFTCTLACLYRKKDECGIEQGGPMYFIEVGLGKYFKPLAIMFAVCGMVG
                     CLALFQVNQLSGLLENDWGIGKLYTGTACMLAVGIVILGGIVRVGQVTSRIVPVMFSV
                     YVISALYIILSNASIVPGLFKTIIASAFGGEALVGGGTAIVFKEVLINGIKRAAFSNE
                     AGIGTAPMAHGAAKTNEPVREGLIAMLGPFLDTNLVCTMTALVILATGAVPQEDGVVM
                     TVNAFEHGMPGMGRYVMTLIIIMFSISTMISYSYYSLKCSRYLFGYKFGSYYVYVYIL
                     SLPVAAVWSQDIVINLLDTSFALMAIPTLVGALLLSPNVIKASRDYFARLKSGFRE"
     gene            4586..4798
                     /locus_tag="ENO07_02315"
     CDS             4586..4798
                     /locus_tag="ENO07_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HDS00830.1"
                     /translation="MRTNVGRIERIFRIIVGLFIVSLIFWGPQSYWGLLGIIPILTGL
                     SGWCALYRILGVSTHKPTSAKESPEV"
     gene            complement(4835..5353)
                     /locus_tag="ENO07_02320"
     CDS             complement(4835..5353)
                     /locus_tag="ENO07_02320"
                     /inference="COORDINATES: protein motif:HMM:PF13590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HDS00831.1"
                     /translation="MLRTIAVSAFLGLLVLGGCASKTVMSDFDRNSDFESYRTFGFIP
                     YNDKLDPSSPLNKRSMRVRIEDAIRDELESRDYRFIDNGNPDIRIAFHASAREQIMVD
                     HYGYSSWQGPGQTDISRYKEGTLVIDFVDPDQKQLVWRGWAVGVIIGPEDTNEQISKT
                     VKRIIDKYPPKE"
     gene            5620..7053
                     /locus_tag="ENO07_02325"
     CDS             5620..7053
                     /locus_tag="ENO07_02325"
                     /inference="COORDINATES: protein motif:HMM:PF03051.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDS00832.1"
                     /translation="MRITGFLSVICLILIGAVLPVSADNSEGGITPEMIEKMRATSKF
                     EGPWKAIYNSLMNNDIKELALDHEKVVEHQDLFNVTIKTKGVTNQKSSGRCWLFASLN
                     LMRPIMIEKYDLNNFEFSQSYLAFWDKLEKANMFLEAIIELRDRDPLDRELREFLRSP
                     FGDGGWWYYTVDLIEKYGCVPKEVMPETQSTSNTGMMNKIISTKLRKCASELRQLYSE
                     GAGLEELRNAKENMLMEIYRMLTLNMGLPPTEFAWRYEEKDSTVSEPQKYTPQEFYRK
                     VVDLDLRDYVTMTDHPGRPKFEMYSVKYTRNMYDREDGVFMNLPIDTLKAYALRTLLD
                     SEAVVFACDVGEDNANDKGIFRPGIYDYEAVYGIDFEMDKEEKFIYGDLSANHMMLIS
                     ACDTLNGQVQKWKVENSWGTDRGDSGFWTMYDDWFDDNVFEIIVHKKHLSGDLRKLLK
                     KEPIELPLWDPYSRFIRERHLQDAYSE"
     gene            7135..7893
                     /locus_tag="ENO07_02330"
     CDS             7135..7893
                     /locus_tag="ENO07_02330"
                     /inference="COORDINATES: protein motif:HMM:PF00753.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDS00833.1"
                     /translation="MVNSGRYQGDNPENVTISIVFDNNRISDLFESAWGFSCFIEGAA
                     KNILFDTGGDGELLLRNLKKLDKSPSDIGLIVLSHKHSDHVGGLGKILEENGYLDVCM
                     LPSFPDSIKEVVRSLGGRLIETGDSGEIIEGVYTTGKMGSYIEEQSLIIDTDKGLILI
                     TGCAHPGIEKIAERAKQILPKEILLIAGGFHLRDKSENEIRAVSESLKNTGVMYAAPS
                     HCSGAKAFEIFREAYGDHYINSGAGKVIPCAGLK"
     gene            complement(7899..10586)
                     /locus_tag="ENO07_02335"
     CDS             complement(7899..10586)
                     /locus_tag="ENO07_02335"
                     /inference="COORDINATES: protein motif:HMM:PF00082.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00834.1"
                     /translation="MKVNPYRTIYLAALGAGLVMLLTISFVFGKSVDDQKVSGKIEVD
                     RVIVKFLDEYHVRYLHGRLASPSVDLSPIENTVQVESGKSIKPLFIRDQAEIDSERLE
                     LESEKGLSLPDLNSYYQVELTDYEAARELVGELENNPLIEKAYIRPRSYPAEDIPPTT
                     PDFMSEQGYLFSAPDGVGADYAWTVPGGKGENVQVIDIEGNWNFDHEDFGENIDTLLF
                     GLPVNAPGWASHGSCVIGIIGADSNAYGITGIAHKAEISTVSVGAVGIATAVDFAGSY
                     LSPGDVILIELNTPGPRYDFEYRPDQAGYVPEEYFDETFDAVQLASAEGIIVVAVAGN
                     GAENLDDPIYENKFNASFRNSRAIIAGAGAPPSGNWGQDRSRLYFSNYGSRVDLQGWG
                     KEVAASGYGVLFNGDGDYRQHYTHKFSGTSAAAAIVTGVVASLQSAYIQAFGEPMNVE
                     DLVNLLDLSGTLQPNPTENIGPRPDLELALQSMQAPGHVAVSPRFVEYTITDGGTGQT
                     SIFLLNNYSDRAISYNLTFDDTIEGWTTGGWLDVSPQSGSVAALGGEMIHFYFDGTII
                     PGSVMPYKAIAEIELNLNPGMDTILVPIFFNLECNDTSYYVYDSDEGVISFEWIDITG
                     VGTMILESEYYNPEVSGIALDDGTAGPYQLGFSINFYNNYFNNINICTNGGVSFVEEN
                     LTINGYFDDIYFPSPGFDAFLSPFWNDLTLDQSYHGNGAIYYYQSPSNDTFIVSYERV
                     GNLVEAGDTTITFQIILTANGYIRFQYKDIGIGGAATTSLVGFSLDQDCSYQKFFDRY
                     QSIQNMPHDSFAVEFMPNYDFTYLIGDTNNDGSINISDAVHIVNFVFISGPPPLPYES
                     GDTNCDGYVNVSDAVWIINYVFVNGNAPGDTDGDGGEDC"
     gene            10915..11748
                     /locus_tag="ENO07_02340"
     CDS             10915..11748
                     /locus_tag="ENO07_02340"
                     /inference="COORDINATES: protein motif:HMM:PF00881.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015301437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDS00835.1"
                     /translation="MSGILFLKTADLNGVTGFYLNSVGCQLWLEQPDCRIFRHGNFLF
                     GFCRGDSAETNGVLTFFYPSKGQVDNMYSGLQDIAREAPRMNEKYNIYHFYAKDPEGR
                     DVEFQYFNHTLKGYLAGDDLLLTRRSMRKFLDQDVSDQMIRLVVDMTRFAPTSRNTQG
                     FYFKIIRDPEMLQKLSELRGSSSAPIANASVAIAICSDSEITKRHIQDACIGAYHFML
                     SAWFHGLGTVWIAAMDREDVKQMLKIPQDHYIATITPLGYPEVTFKQPPERKGVDWYI
                     R"
     gene            complement(12027..15221)
                     /locus_tag="ENO07_02345"
     CDS             complement(12027..15221)
                     /locus_tag="ENO07_02345"
                     /inference="COORDINATES: protein motif:HMM:PF00873.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDS00836.1"
                     /translation="MSLIKSSIKYPVTVVVAVLIITISGFLALKQVPIQLTPEVQQPI
                     ITVSTTWVGASPEEIEREIIEEQEEYLKSVEGVEEMRSESQDGRGTITLEFPVGTDLT
                     GAVVRVTNKLNEVPSYPQNAERPVVRSSGETEGAIAWFIVRALDSNLYIPHQERLIED
                     IVKPRLERVEGVASINIYGGLEQELHVVFDPDLLASSGITVDMLANALRAENRDISAG
                     DFGEGKRKYVVRTTSRFESIEDVEETVVAVQNGVPIRVRDVAEVSLDFEKPIAMVRNL
                     GSPAIAFNAQRQVGANVLEVLDDLLKQVDILNREVMNPRGMNIVNAYNETTYINSAID
                     LVFSNIYLGGFLAILVLFIFLRSASAIAVIALSIPISIISTFLAMSLFGRTINVISLA
                     GMAFAVGMVVDSSIVVLENIYRHMQMGKSKWQAAADGAREVWGALLASTVTTIAVFLP
                     IMFVEEQAGQLFQDIAIAISSAIVISLLVSITVIPTLSSRALKVSSKLQGSDQHKSLL
                     GRFAARVAAFVDFINARGARRLGTIIGIVVFAMFFTWLLLPDAEYLPNGNRNLVFAFM
                     LPPPGYNIDEMVRAGQTIESQIKPLWEAEGEEAEEMPGGGIENFFFVAFSGQAFFGMA
                     SRDHERASELVQVANKAIFSIPGSIGFASQTSLFGRGFAGTRSVRIDVTGPELGKVLQ
                     IAGQVFGQVGQILPGSNSRPIPGLDLGNPEVRVIPDRVRAADVNLTASQIGLAVNALV
                     DGAIVSDYYHNGKELDLIIKGRENWTRHTQDISQLPLATNSGKLVTVGDVADVVMRQG
                     PVQINHVERQRTVSIETVLPDEIPLEKAMVMLENDIISHLRSQGQVGGLYDINLSGTA
                     DDLSKLRAELTTDFHLAIILTYLLLSALFQSFTYPLVILLTVPLATFGGVLGLNVVQL
                     FDSSQQFDILTMLGFVILVGTVINNSILIVYYALQLMREGWDARSAVKESVRVRVRPI
                     FMSTGTSTLAMLPLIIMPGAGSELYRGLGSVVVGGLAVSTVTTLILTPLVFSYAIELT
                     VRIKGFLGMKRKAVSTAPAVDSPGSTTAAVERDS"
BASE COUNT         4083 a         3726 c         3867 g         3947 t
ORIGIN      
        1 aaattgggcc gccactacat cgttgaaaca acatccttga ggcggccctt tttgttcgta
       61 tataaccgca atgccccgtg cctcatcaat tcctgacagc attatttact tgccagaaac
      121 ccgtaattct gataacatag acatcagggt attatcacaa agggagtcaa gattagaatc
      181 aaggaattgt gggaacggtt taaatcccat tttcggccta aacgtatcaa ggatttctgg
      241 gcgcattacg gccttggatt gatagaccgt attggcaaga accatgtctt tctggaggcc
      301 agcggcctgg cattctcgat catcgtctgt atagtaccct ttgtattaat tgtattcgct
      361 gtcctgggct ttgtgttaga gagccctact atcagggtgg agatcaataa tttcattgat
      421 cgcctgattc cgtattcaga tgcggccgag actgtaaaac agattgtttt tgaacgtgcc
      481 caggaattca aggtgaacaa aaatattgcc gggatatttg gtatcctggg actcctgtgg
      541 gcctccagca ggctcttcag cagcatgcga acaatattga atatctccta caagaaccgt
      601 ctgggaggtt cgatattcgg agatgttgtg gccaagttgc gggatctggc gatggtgctc
      661 ctggtgcttt tttattttct gctggccaca accgttatac ccggcctggc agtactggag
      721 aaagtgactg aggactcaac catattggag tttttgcata tacaatttgt gggtgatatt
      781 atcgtccagg cggcttcatt tatcatactg acgtttgctt tttttgtaat atactacttt
      841 gtcccccagg ctaagattcc gctgaaggta attttggtta gtgccctgtg gggagcgttt
      901 ttctggggca tagcggaata tctcttccga atatacataa ataatttcgt taacctgaag
      961 cggatttacg gggcttatgt gctctttatt gtcgctgttt tctggatttt ttatacttct
     1021 gtggttttaa tcgtgggagc agaaatcggc cagctatacc gtgaaagact gagaaaaaga
     1081 gagaaggagg ctcggaaaga acaggttgcc gagacatgat atgttcaaaa aagacttgcc
     1141 taagtatata acacttaata gattcatgat atcgcgaaag aaagctaact gttaatcgaa
     1201 ccggggtctc aaggaggagc ccatgaaagc gctcgatgta attttcttga ttctgctcgt
     1261 agtaggcgga ctcaactggg gactggtggg aatattcaat ttcaacctgg tcgccgcaat
     1321 ttttggtgaa atgtccgtca taagccgcat tatctacatt ctcgttggga ttgcggcact
     1381 ctataacctg gtgctgtgga aatcaatgca gcagcgctgg ggaagaggac atgctccggc
     1441 aacggcctga acatcgtgaa agacatccgg gcgctcggct gattttaagc acaacaaggt
     1501 atagacattt atgccgggtg caagtgcatc ggcttctaaa aacatgattg gccggcagat
     1561 attttcctgc cggcctttct tgcgtgaatt ccgtgcaaaa aaacattgct gttcttgtgc
     1621 atttatggtt tatttttctc aacagtttat ttttccaggg gcgtaacaat atctaaaaag
     1681 gcggttataa tgcccgatgt actgcaaaca gaaaaggcta tcaaggtcgg gatcagcgca
     1741 tgtcttctgg ggcagatggt gcgctatgat gccggacaca agcgcgaccg ctattcaacc
     1801 gatgtgcttt ccaattactt ccagttcgtt tcagtttgtc ccgaagttga ggttggcatg
     1861 ggtgtgccgc gcgaggcggt cagattggtg ggtacagccg aggaaaccag gatggtcggt
     1921 aacaagaccg gcaaggactg gacggaaaag gtggtggcct ttaatgccaa acgcgcaaat
     1981 gagctcgaga aggaaaacct gagcggtttt attctgaaga aagactctcc gagctgcggg
     2041 atggagcggg ttaaggtcta taatgagaaa ggtatggccg agaaaaatgc tgtgggtatg
     2101 tggccgcggg ctttgaaaaa acagttccca atcatgccca tggaggaaga ggggcgtctc
     2161 aatgatcccc gattgcgcga aaactttatc gtcagggtat ttgcctacca taggttgcag
     2221 aacctctttg acgatagata cagccggggg aagatggtac ggttccatac cgcccacaag
     2281 ctgcttttga tgtcccatag tcccaaacat tacaaggaac taggaaagct ggtcgcggag
     2341 gtgaagaagt attcgccctc ggagatgcgg gaaaagtaca gcgcggtttt tatggagggt
     2401 ctgggctata agacgacagc aaagaagaat gtcaatgtgc tacatcatat tctcggctac
     2461 ctgaaaacaa atctgacgtc atcggagaaa aaggacatac tgcaggttat cgatgattac
     2521 cacaaggaat tgactccgct tgtggtgccc ctgacgctct tacgtcatta tgtaactata
     2581 catgatattg aatatataaa agaccagata tatctaaccc ctcatccgaa ggagctcatg
     2641 ctccgcaacc atgtataaaa gcctgctcaa tgcccgaatg catccttgag catctgttcg
     2701 tggtcaaagg ccagtttcat tgcgaaaaag tcctcggtta agattagttc agcggcatca
     2761 tcggccgctt ttggctcacc gctgacgggt ctggccagat atgttaccga cataacacgc
     2821 ccgcgcggat cccgatccgg atcggaatat actccgacca gtctttttaa ttcgactctc
     2881 acacccgttt cttcacgggc ttctctgaca gcggcctcct cgacagtttc attctcctcc
     2941 accattccgc ccggaagcgc ccaatagccc tggtacgggt cattctttct ctttatcaaa
     3001 atgatctttc catccgggta ttggatgatc aaatctgtag caacaatcat cttaaacaca
     3061 ttcagcctcc ttattccctg aatcctgact tcagccgggc aaaataatct ctcgatgctt
     3121 ttataacgtt cggcgagagc aaaagtgcgc cgaccagggt cggaatggcc atgagcgcaa
     3181 aggatgtatc cagcagattg attacaatat cctgagacca taccgccgca accggcagac
     3241 tcaatatata gacataaaca taatagctcc cgaatttgta tccaaaaaga tagcgcgaac
     3301 atttaaggct gtaatatgaa taggaaatca tggtggagat cgagaacata ataatgatca
     3361 atgtcataac ataccggccc atccccggca tgccatgctc gaatgcattc acagtcatca
     3421 ccacaccatc ctcctggggc acagctccgg tggcaagaat aaccaatgct gtcattgtgc
     3481 ataccagatt agtatccaga aagggcccca gcatggcaat cagaccctcg cgcaccggct
     3541 cattcgtctt ggcggcgccg tgggccatcg gtgcggtgcc gatccccgcc tcgttcgaga
     3601 aagcggctcg ttttataccg ttaatcagta cctccttaaa tacgatagca gtaccgccgc
     3661 ctaccaaggc ttctcctccg aaggctgagg ctattatggt tttgaacagc ccgggcacaa
     3721 tggaggcatt tgagagaatt atataaagcg cggaaatcac gtatacagaa aacatgaccg
     3781 gtactatgcg gctggttacc tgaccgaccc ggacgatgcc ccctagaatg acgattccaa
     3841 ccgcaagcat gcaggccgta ccagtataga gcttgccgat gccccagtcg ttttccagga
     3901 gaccggagag ttggttaacc tgaaaaagcg cgaggcagcc gaccatcccg cagacggcga
     3961 acatgattgc cagcggcttg aagtatttac ccaggcccac ctcgatgaaa tacatcggac
     4021 cgccctgctc tatcccgcac tcatctttct tgcgatacag acatgccagg gtgcaggtga
     4081 aatacttggt ggacatgccc actatacccg ccacccacat ccagaatatt gccccggggc
     4141 cgccgatggt aatgcctatg gccacgcccg agatattgcc cattccgatg gtggccgaga
     4201 gtgcggaaga aagagcctga aaatgattta tctcacccgg atcatcggga ttgtcatatt
     4261 tgccgcgaag aatatttacc gcatgtttga ggtgacggaa aggaacgaat cttcccacga
     4321 tagtgaaata gactccagcc gtgatcagga gcacgaccag tggaatcccc caggcaatat
     4381 cgaccgccct ggacagaata gaatcgaggt attgcaatca aaactcccgt aagaatggaa
     4441 tcggataccc gcttgattga gaattataat atgaattcat cggtagtatt gcaaaataaa
     4501 atcgcgataa ccttaatgat cgatgaacag ttgattgcgt taactgttgt aagtggtaga
     4561 actgaaaaaa gggagagtga tatttatgcg gaccaatgtc ggaagaattg aaagaatttt
     4621 caggattatt gtgggcttat tcatagtgtc tttaatcttc tggggaccac aatcctattg
     4681 gggacttctg ggtattatcc cgattctgac cggattgagc ggatggtgtg cgttgtaccg
     4741 gatccttggc gtctcgacgc ataaaccgac cagcgccaaa gaaagcccgg aagtataaaa
     4801 agtaagagcc gggttctaat cccggctttc ttggttactc tttgggaggg tacttatcaa
     4861 ttattctttt cacagtttta ctgatttgct cattggtgtc ctccgggcct atgataactc
     4921 cgacggccca tccgcgccag accagctgct tctgatcagg atctacaaaa tcaattacca
     4981 gggtaccttc tttataacgt gaaatgtcgg tctgcccggg tccctgccag ctgctgtaac
     5041 cgtaatgatc gaccattatc tgttccctcg cactggcatg aaatgctata cggatatccg
     5101 ggtttccatt atcaatgaag cggtagtctc tggattccag ctcatctcgg atggcatcct
     5161 cgatcctgac ccgcatggat cttttattca aagggctcga gggatcgagt ttatcattgt
     5221 agggaatgaa cccaaaagtc cggtaggatt caaaatcaga atttctgtcg aaatcggaca
     5281 ttaccgtttt ggaggcgcat ccccccagaa caagcagccc caaaaacgca cttacagcta
     5341 tggttctaag catgatttat ctcccattta tacactgata tttgaatcgg aatataaaag
     5401 cggataattt agggtgaaat ataaaactgc cgcaaaatgg cggatttaaa tgcccggccg
     5461 aatcgccaat ctgctcagga caattattaa attcagatca gggggcgatt aattctatca
     5521 aaaaacgaaa tttcctgtag cgggttgggc ataattttcg ttttatttag acaaatagaa
     5581 tttattgcct gttataatac aattcatcaa ggagaaacta tgagaatcac aggctttctg
     5641 agcgtaatct gtctaattct gattggtgcg gtcctgcccg tttccgcaga taattccgaa
     5701 ggcggaatca cccccgagat gatagagaaa atgcgcgcga cttcaaaatt cgagggcccc
     5761 tggaaagcga tttataactc gcttatgaat aacgacataa aggaactggc gctggatcat
     5821 gaaaaagtcg tcgagcacca ggatttattc aatgtgacaa tcaagacaaa gggcgtaaca
     5881 aatcagaaat cctccgggcg gtgctggcta tttgccagcc tgaatcttat gcgccctatc
     5941 atgatagaaa aatatgacct gaacaacttc gagttttcgc agagttatct ggctttctgg
     6001 gacaagctgg aaaaagccaa tatgttcctg gaggcgatca tcgagctgcg ggacagggat
     6061 cctctggatc gggagctgcg tgaatttctg aggagcccct ttggcgacgg cggctggtgg
     6121 tattacactg tggacctgat agaaaaatac ggatgtgttc ccaaagaagt aatgcccgag
     6181 acccagagta cctccaatac cggtatgatg aacaagatca taagcaccaa gcttcgcaaa
     6241 tgcgcttcgg agttgaggca gctttattct gaaggcgccg ggctcgagga attgaggaac
     6301 gcgaaggaaa acatgctgat ggaaatctat cgcatgctga cgcttaatat gggcctgccg
     6361 ccgacagaat tcgcgtggcg ctacgaggaa aaggattcca cagtctccga gccgcaaaaa
     6421 tatactccgc aggagttcta tagaaaagtg gttgatcttg atctgcgcga ttatgtaacg
     6481 atgacagatc atcccggccg gcccaagttc gagatgtatt cggtaaagta tacccgaaat
     6541 atgtatgacc gcgaggacgg tgtatttatg aacctgccga ttgacacgct caaggcctat
     6601 gcattaagaa cccttctgga tagcgaggcg gtagtttttg cctgcgacgt cggcgaggat
     6661 aacgctaatg ataaaggtat atttcgcccg ggtatctatg actatgaggc ggtgtacggc
     6721 atcgattttg agatggataa agaggaaaaa ttcatctacg gcgacttatc cgctaaccat
     6781 atgatgttga tctcagcctg cgatacgctc aacggccagg tgcagaagtg gaaggtggag
     6841 aacagctggg gcacagacag gggcgacagc ggtttctgga ccatgtatga cgactggttc
     6901 gatgataatg tatttgaaat aatcgttcat aagaagcatc tctccggtga tctgcgtaag
     6961 ctattgaaga aagaaccgat tgaacttccg ttatgggatc cctactcgcg ttttattcgg
     7021 gagagacatt tacaggacgc atattctgaa taatggggaa ttttcccact ataatgggtt
     7081 caatacgggt gcactcttat actggttcga caaaaggtgg tgagttaagt caatatggta
     7141 aattcaggaa gatatcaggg cgataatcca gaaaacgtca caatcagcat agtctttgat
     7201 aataacagga ttagtgattt attcgagagc gcctggggat tttcctgctt cattgaagga
     7261 gcggcaaaga acatcctgtt tgacacaggc ggcgatggag agctgctctt gcgaaatctc
     7321 aagaagctgg ataagagtcc ctctgatatc gggctaattg tcctgtctca taaacacagt
     7381 gatcatgtgg gcggtttggg gaaaattctg gaggagaatg gatatctgga cgtctgtatg
     7441 ctgccctcat tccctgatag tataaaagaa gttgtcaggt ccttgggcgg ccgcctgatt
     7501 gaaactggag attccggtga aattattgaa ggagtttaca ctacgggaaa aatgggatca
     7561 tacatcgaag agcaatcctt gattatagat acagacaaag gactcatttt gatcacaggc
     7621 tgcgcccatc cggggatcga aaagatagct gaacgggcaa agcaaatatt acccaaagaa
     7681 atcctgctta tcgcgggagg atttcacctc agggataaaa gtgaaaatga aatcagggcg
     7741 gtgtctgagt ctctgaaaaa tacaggcgta atgtatgcag ctcccagcca ttgttcaggc
     7801 gcaaaggcat ttgaaatatt tcgcgaggca tatggcgacc attatattaa ttccggagcc
     7861 ggaaaagtaa ttccctgtgc cgggctcaaa taaacctgtc agcagtcctc cccgccgtca
     7921 ccgtcagtat cccccggggc attgccgttt acaaagacat aatttattat ccagactgca
     7981 tcgctgacat tgacatagcc atcgcagttg gtatctcccg attcgtaggg aagcggggga
     8041 ggtcctgaaa tgaagacaaa gttgactatg tggacggcat cgctgatgtt aatggagccg
     8101 tcattgtttg tgtccccgat caaatatgtg aaatcgtaat tgggcatgaa ttcgaccgca
     8161 aatgaatcat ggggcatatt ctgaattgac tgataacggt caaaaaactt ctggtaactg
     8221 caatcctgat cgagggaaaa gcccaccagc gaggtggtag ccgccccgcc gataccgata
     8281 tccttatatt gaaaacgaat atatccattg gcggtaagaa ttatctggaa cgttatagtg
     8341 gtgtctcctg cttccaccag gttgccgact cgctcgtaag acactatgaa cgtatcgttg
     8401 gaaggggact gataatagta tatggcgccg ttgccgtgat agctttgatc cagggtcaga
     8461 tcgttccaaa agggtgagag aaaagcatca aatccgggag aggggaagta aatatcatca
     8521 aaatagccgt ttatggtcag gttctcttct acgaatgaga ctcctccgtt ggtgcagata
     8581 ttgatgttat tgaagtagtt gttatagaag tttatgctga agccaagctg atatggtcct
     8641 gcggtgccgt catccagggc aataccggaa acctccggat tataatattc cgattcgagt
     8701 atcatagtgc cgacgccggt tatgtctatc cattcaaagg aaatcacacc ttcgtcagag
     8761 tcatagacat aataactggt atcgttacat tccagattga aaaatatcgg caccaggata
     8821 gtgtccattc ccggattcag gttgagttca atttcagcta tggctttata aggcataaca
     8881 cttccgggga tgattgttcc gtcgaaatag aagtggatca tttcgccccc gagagctgct
     8941 actgatcctg attgcgggct tacgtccagc catcctccgg tcgtccagcc ctcaatcgta
     9001 tcatcgaagg taagattgta gctgatcgcc cggtcggaat agttattgag caggaagatg
     9061 ctggtctggc ccgttccgcc gtctgtgatc gtgtactcga caaaacgcgg actgacggca
     9121 acatggccgg gagcctgcat cgactgcagg gccagctcca gatcgggacg ggggccgata
     9181 ttttctgtcg ggttgggctg caatgttccg cttaagtcaa gcaggtttac cagatcctcc
     9241 acgttcatcg gttctccgaa cgcctgtatg tatgcggatt gcagcgaagc aaccaccccg
     9301 gttacgatag cggcggcagc cgaggtgccg ctgaatttat gtgtgtagtg ttgccggtaa
     9361 tccccgtcgc cattaaaaag tactccgtat cctgaggcgg ccacctcttt tccccagccc
     9421 tgaagatcga ccctcgagcc gtaattggag aagtataatc gcgaacggtc ctgtccccaa
     9481 ttgccggagg gcggggctcc ggctccggca ataatggcgc gggaattgcg gaatgaggcg
     9541 ttgaatttat tttcatagat cggatcgtca agattctcgg cgccgttgcc ggcaactgcg
     9601 accacaatta tgccctcggc cgaggccaat tgcacagcgt cgaatgtctc atcgaaatat
     9661 tcttcgggca catagccggc ctggtcggga cggtactcga aatcatatcg gggcccggga
     9721 gtattgagtt caatcaggat gacatcgccc ggcgatagat atgagccggc gaaatcaacg
     9781 gccgttgcga tcccgactgc gcccaccgat acagttgaga tttccgcttt gtgagcgata
     9841 cctgtgatgc cataggcatt ggaatcagcc cctattatgc cgatgacaca ggagccgtgg
     9901 gaagcccatc caggtgcatt gaccggcaga ccaaacaaaa gcgtatctat attctcgccg
     9961 aagtcctcgt ggtcgaagtt ccagtttcct tcgatgtcga ttacttgtac attctcgccc
    10021 tttcctcccg gaacagtcca ggcatagtca gcccctactc catccggtgc cgaaaaaagg
    10081 taaccctgtt cggacatgaa gtcaggcgtg gtgggaggaa tatcctcagc gggataggat
    10141 ctgggacgga tatatgcttt ttctattaag ggattgtttt caagttcccc gaccagctcc
    10201 cgcgccgcct cataatcagt taattccacc tgataataag aattcaaatc ggggagcgaa
    10261 agaccctttt ccgattcaag ctctaacctc tcgctatcga tttcggcctg atcccgtatg
    10321 aatagcggct tgatcgattt tccactttca acttgtacgg tattttcaat tgggctgagg
    10381 tcgacggacg gtgacgcaag gcggccgtgg agatatctca catgatattc gtcgaggaat
    10441 ttgacgatca ctctgtcgac ttcgattttc ccgctaactt tttgatcatc aacggatttt
    10501 ccaaaaacga aagaaattgt caacagcata accaggccag cccctaatgc cgccaaatat
    10561 attgtcctgt atgggtttac tttcatgtca gaatctctcg gtaagaaagg gtttcctcag
    10621 tccaatataa gtcatcgata taggctgtca acctctttac ggctgcgcac agcaattttc
    10681 gagaaaagaa ctgattctga ttgctgtagc ggatcttgta tgcgaactcc cgaattaaat
    10741 ccgagagtat ttttcaacgg gtacaattat tgtcgactga tctgaattca atgtttccga
    10801 ggataactcg ggatccgaat atttgcagta tgagatttgc atattaatct tgatgatgcc
    10861 ccgatgcggc cttataatta ctacccagtt aagattatgc cgaaaggagg aaggatgagc
    10921 gggatattat ttttgaaaac tgccgatttg aatggtgtaa ccggattcta cctgaacagc
    10981 gttggatgcc agctctggct ggaacagccg gattgcagga tattccgtca cggcaatttt
    11041 ctcttcggtt tttgcagggg ggattcggct gaaaccaacg gcgttctgac atttttttat
    11101 ccttccaaag gccaggttga taatatgtat agcggactcc aggatattgc cagggaagct
    11161 cccaggatga atgagaaata taatatttac catttttatg ccaaggatcc ggaggggcgc
    11221 gatgttgaat ttcagtactt caatcatacg ctgaaaggtt atctcgccgg ggatgatctt
    11281 ttgcttacac gcagaagcat gcgcaagttt ctggatcagg atgtttccga tcaaatgatc
    11341 aggttggttg tggatatgac ccgtttcgcc ccgacctccc gcaataccca gggattttat
    11401 ttcaagatta tccgggaccc tgaaatgctt caaaagctct cagaactgcg gggcagctcg
    11461 agcgctccga ttgcaaatgc ttccgtggca attgctattt gttctgactc ggaaattacc
    11521 aagcgccata tccaggatgc ctgcataggg gcttatcatt ttatgctgag cgcctggttc
    11581 catggactgg ggactgtctg gattgcggcc atggacaggg aggatgtgaa gcagatgctc
    11641 aagattccgc aggatcatta tattgctact ataacgcccc ttggatatcc tgaggtaaca
    11701 tttaaacagc ctcccgagag aaagggcgtg gattggtata tcagatagtg attcaaatct
    11761 ttgaaatgaa ttcctgtttc aatagaagca tctttctgct ttcgatagca tgtttggttt
    11821 taatctgata gaagtaaacg cctgatggaa gagccttcgc atcccattaa atcgtgtgaa
    11881 agcctgctct caagagtcaa tggatccgat aaagttaaag atgttggaat agcctgttgt
    11941 ctgtagctta atgtataacg cggatacagg acttctgttt aaaaaaaacg aaaaaattat
    12001 tcgataatca gatcgtcaaa tcagattcag gaatctcttt caaccgccgc tgttgtgctg
    12061 ccgggactgt ccactgccgg cgctgtactg accgcttttc tcttcattcc cagaaaaccc
    12121 ttgatccgga ctgtcagctc aatggcatac gaaaatacca gcggagtcaa tatcagggtg
    12181 gttacggtgg ataccgccag accgcccaca accaccgagc cgagaccgcg ataaagctcc
    12241 gatccggcgc ccggcattat gataagcggc agcatagcga gagtcgatgt tccggttgac
    12301 ataaagatgg gacgtacgcg cacccgaaca gattccttga cggccgaacg ggcatcccag
    12361 ccctctcgca tcaattgaag agcataatat acgatcagga tggagttgtt gatcaccgtt
    12421 ccaaccagga tcacaaaacc aagcattgtg agtatatcga actgttgtga actgtcgaat
    12481 aactggacaa cattcaatcc cagtaccccg ccgaatgtag ccaggggcac ggtcaataat
    12541 atcaccagtg ggtatgtgaa tgactggaaa agcgcggata gaagcagata tgtcagaatg
    12601 atcgccaggt gaaaatctgt tgtcaattct gcacgcaatt tacttagatc atctgcggtt
    12661 cccgagagat ttatgtcgta aaggccgcca acctggccct gacttctcag gtgactgatg
    12721 atgtcatttt caagcatgac catagctttt tcaagcggga tctcatcggg cagtacggtt
    12781 tcgattgaaa ctgtccgctg cctttcgaca tgattgatct gcaccgggcc ctggcgcatg
    12841 acgacatcgg ccacatcccc gaccgtaacc agttttccac tgttggtggc aaggggcaac
    12901 tgagatatat cctgcgtatg acgggtccag ttctcacggc ccttgataat cagatcaagc
    12961 tctttaccgt tatggtaata atcgcttacg attgcgccgt caaccagagc attaacagcc
    13021 agtccgatct ggctggcggt aagattgaca tccgccgctc gcacccggtc aggaataact
    13081 ctgacttcgg gatttcccag gtcgagcccg ggaattggac gggaattcga accgggcagg
    13141 atctgtccca cctggccgaa tacctggccc gcaatttgca gcactttacc caattcaggg
    13201 ccggtcacat caatccgcac cgatcttgta ccggcaaagc cgcgcccaaa caatgaagtc
    13261 tggctggcaa aaccgatcga tcccgggatc gagaatatcg ccttgttggc cacctggacc
    13321 agctccgagg cgcgctcatg atcgcgcgag gccatgccga aaaatgcttg tcccgaaaag
    13381 gccacaaaga agaaattttc tatgccgccg cccggcattt cttctgcctc ttcgccctct
    13441 gcctcccaga gcggcttgat ctgggattcg attgtctgcc ccgccctgac catttcatca
    13501 atgttgtatc cggggggagg gagcatgaat gcaaatacca gattccggtt gccgttaggg
    13561 agatattccg cgtcgggcaa taacagccag gtaaagaaca ttgcaaacac tacaatcccg
    13621 ataattgtac ccagcctgcg ggcgcccctg gcgttgataa aatccacgaa tgcggccacg
    13681 cgggcggcaa acctgcccag cagtgattta tgctgatcgg aaccttgtaa tttgcttgag
    13741 accttaagtg cacgggagga taatgtcggt atcacggtaa tcgaaacaag aagtgagatt
    13801 acgattgcgc ttgatattgc aatagcgata tcctggaaca gctgcccggc ctgttcttca
    13861 acaaacatga ttggaagaaa tacggcgatg gtcgttacag ttgaggccag caaggcaccc
    13921 cagacttcgc gcgcgccgtc agcagcagcc tgccatttgg acttgcccat ctgcatatga
    13981 cggtaaatat tctcgagtac cacaatggag ctgtccacca ccattcccac cgcgaaagcc
    14041 ataccggcaa gcgaaatgac attgatggtc cgcccgaaca gagacattgc gaggaatgtg
    14101 ctaattatcg agatcggaat cgaaagcgca atcaccgcta tggccgaagc cgatctcagg
    14161 aatatgaaca gcaccagaat tgccagaaag ccgcccagat aaatattcga gaataccaga
    14221 tcaatggctg aatttatgta ggtggtttca ttataggcgt taacgatatt catgccacgc
    14281 gggttcataa cctcgcggtt gaggatatcg acctgtttca agaggtcatc cagcacctcc
    14341 aggacattgg ctccaacctg gcgctgcgca ttgaaggcta ttgccggact gcccagattg
    14401 cgcaccatag ctatgggctt ttcgaaatct aggctgacct cggccacgtc gcgaacccgg
    14461 atgggcacgc cattctggac cgcaaccacg gtctcttcga catcctcgat cgattcaaag
    14521 cgggaggttg tgcgcaccac atattttctt ttgccttcgc cgaagtcacc ggctgaaatg
    14581 tcgcggtttt ccgcccgtaa agcattggcc agcatatcca ccgttattcc ggaagaagcc
    14641 agcagatcgg gatcgaagac gacatgcagt tcctgctcca ggccgccgta gatattgatc
    14701 gaggcaacac cctcgacacg ttccagtcga ggtttgacaa tatcttcgat gaggcgttcc
    14761 tggtgcggta tatataaatt cgaatcgagc gcgcgcacaa tgaaccaggc tatcgcgccc
    14821 tcagtttcac cggatgatct tactaccgga cgttccgcgt tttgggggta ggagggtacc
    14881 tcgttgagct tgttggtcac tctcactact gcgccggtga gatcggtgcc gaccggaaat
    14941 tcgagtgtaa ttgtccctct accatcctgt gattcagaac gcatttcctc cacgccctcg
    15001 acagatttaa ggtattcctc ctgctcctcg ataatttctc tctcaatttc ttcgggagaa
    15061 gctccaaccc aggtggtgct gacagtgata ataggctgct gtacttccgg cgtaagctgg
    15121 atcggcacct gctttaaagc cagaaatccg gagatggtta tgatcaatac ggccactaca
    15181 acggtgaccg gatattttat cgaggatttt attaagctca ctttgttatc ccttaattag
    15241 ttttccttgc agtagaatta atctttctgg ctcacctggc ccatgtttgc agtcgattgc
    15301 tgtgcctgcc ctgtggtaac cggactcccc ggaaaaatcc tttcgttgcc ccgcaccacc
    15361 acaggcatgc cctccgaaag accctcaccc tctacagcga tcatcttgcc atcgcttgat
    15421 ttggttacaa ccggaatcgg cacagccttg ccgtccgcga ctgtatagac aagagtctgg
    15481 tctccctgcc ttattatcgc atccttggaa acagccaggc tactgaaggt ctggtccatt
    15541 gaaagagtcg ccctgaccag catcccaccc ccaaggttca tatcttcgtt ggagacttcg
    15601 attatcacgg gaaatgtatg tgt
//
LOCUS       DSEB01000123            1511 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1018811, whole genome shotgun sequence.
ACCESSION   DSEB01000123 DSEB01000000
VERSION     DSEB01000123.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1018811"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            64..951
                     /gene="lipA"
                     /locus_tag="ENO07_02350"
     CDS             64..951
                     /gene="lipA"
                     /locus_tag="ENO07_02350"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007432003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDS00837.1"
                     /translation="MPRKIEKDNIRKPEWFKIKAPLGTNYREVRSLMESYGLHTVCQE
                     ANCPNRAECYGARTATFLILGDICTRGCTFCNVRRGRPREVLQNEPERVAEAAGKLGL
                     KYVVVTSVTRDDLPDGGASLFADTIEAVRAVNRGCRVEVLIPDFRGNFEALKTVLDAK
                     PDVLNHNIETIERLYPAVRKGADYKRSLELISRVRSYSPGIPTKSGLMVGLGEMFDEI
                     RKALEDLHQAGCRLLTIGQYLSPSKKHHPVIAYYSPEEFDELKKMALEIGFDGVVSGP
                     LIRSSYRASMMYSNSGSKH"
     gene            complement(957..1499)
                     /locus_tag="ENO07_02355"
     CDS             complement(957..1499)
                     /locus_tag="ENO07_02355"
                     /inference="COORDINATES: protein motif:HMM:PF03551.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDS00838.1"
                     /translation="MKLNPTDYIIMAYLKSAPMHVYKLSDKLKEDKVDSWIQYSIPHI
                     YYSLRRLKENGLVSVEIKSVKSKPAQKIYSLTDQALKLLDGLQDDNELISGEQFFPFD
                     LIIGLARRLEISGKQVKAMIENRIAFLKSRLEAVQNDFRAKETGTQDMSKGERLAFQH
                     RIRFLKGEIDFYRKSLKEFK"
BASE COUNT          383 a          354 c          385 g          389 t
ORIGIN      
        1 gataatagtt gcgccggaag atgactccgg cgtatatttt tgcgagacag gttttagaaa
       61 aatatgcccc ggaagatcga aaaagacaat ataaggaagc cggaatggtt caagataaaa
      121 gcccctctcg gtacaaatta ccgcgaggtg cgcagcctca tggagtcata tgggctgcac
      181 actgtctgcc aggaagccaa ttgccccaat cgggccgagt gctacggtgc cagaaccgca
      241 acttttctaa ttctgggtga tatatgtacg cgcggctgca cattttgcaa cgtcagaagg
      301 ggcagacccc gggaggttct tcagaatgaa cctgaacgag tggccgaggc ggccgggaaa
      361 ctcggattga aatatgtcgt ggtgacctcg gtaacgcggg atgacctgcc cgatggaggg
      421 gcttctctct ttgcggatac tatcgaggct gtaagggcgg tcaatcgggg atgcagggtc
      481 gaggtcctga ttcctgattt ccggggaaat ttcgaggcgc tgaaaaccgt gctggacgcg
      541 aagccggatg ttttgaatca caatatcgag actatagaac gtctctatcc cgccgtcaga
      601 aagggcgctg actataaacg ctcgctggaa ctaatttcgc gggtaaggtc atactcaccg
      661 gggattccga ctaaatcagg attgatggta ggattaggcg aaatgttcga tgagatcaga
      721 aaggcccttg aagatttaca tcaagcgggt tgcaggctgc tgacgatcgg ccagtatctc
      781 tcaccctcaa aaaaacatca tcccgtaatt gcttactatt ctcccgagga atttgatgag
      841 ctcaaaaaaa tggcgcttga gattggattc gacggcgtgg tctccggccc cctgataaga
      901 tcatcttacc gggcctcgat gatgtatagt aattccgggt caaaacactg atcctgctat
      961 ttgaattcct tcaggctctt cctgtagaaa tctatttcac cttttaagaa tctgatgcgg
     1021 tgctgaaacg caagccgttc ccctttggac atatcttgcg ttccggtttc cttggcgcgg
     1081 aaatcgttct ggaccgcctc caggcggctt tttagaaaag cgattctatt ttcgatcatt
     1141 gccttaacct gtttcccgga gatttccagg cgccgggcga gccctatgat caggtcaaag
     1201 ggaaaaaact gctcgcctga gatgagctca ttatcatcct ggagcccgtc cagcagcttg
     1261 agcgcctgat cggtcagaga gtaaattttc tgagccggtt ttgatttaac cgacttaatc
     1321 tccactgaaa caagaccgtt ttctttcagc ctcctgagcg aataatatat gtgcgggata
     1381 gaatactgaa tccacgaatc cactttgtct tctttcaatt tatcggacaa cttatagaca
     1441 tgcatagggg cgcttttaag ataggccatt attatgtaat cggttggatt cagtttcata
     1501 ctacccaatt a
//
LOCUS       DSEB01000124            1704 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1014411, whole genome shotgun sequence.
ACCESSION   DSEB01000124 DSEB01000000
VERSION     DSEB01000124.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1014411"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..851)
                     /locus_tag="ENO07_02360"
     CDS             complement(<1..851)
                     /locus_tag="ENO07_02360"
                     /inference="COORDINATES: protein motif:HMM:PF01523.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00839.1"
                     /translation="MPEKLEILSRDYILSTFKKALEFSEADQTEIVLESEKLSLTRFA
                     DSKITQNLTTADNKVVVRTIEGNRIGVSVSNSLDLESIKRALKAAREISSVQTPDEQF
                     VSLPQSPPALPVKAYYESTANYSPVNRAEAIGKINDVGQKNNLLSSGAYRITTDRAAV
                     LNSLGTEQFFEGTKAELSLTLSGDNENSGYAIGYNRDVEKIDPTALAEKAADKAVNSV
                     NPIKLESGPYTVILEPSAVGQMVLLFSFLGFGCKTFISQRSFMSGKIGQQIVGNNITL
                     IENPYHER"
     gene            complement(856..1668)
                     /locus_tag="ENO07_02365"
     CDS             complement(856..1668)
                     /locus_tag="ENO07_02365"
                     /inference="COORDINATES: protein motif:HMM:PF01523.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDS00840.1"
                     /translation="MFDFKNELPRIAQEAIQLLSAKECPQKELDLILGGDLVSLQIHE
                     SVGHPLELDRVFGYERNFSGVSFATTDKLGSLKYASDMLTFVADSTYPGGLATYAYDD
                     EGVKTERNILIDKGVLVNYLSDRETAHRIGKKSTACARAEGWMNTPIVRIPNILLLPG
                     ESTLDEMISGIDDGIYVISPNSWSIDDNREGFTLGGEVGWEIKNGRLGDMVKSPRYSG
                     NTVDFWNSCDAVGNSDSWVLWGTPNCGKGQPGQNMRTAQGCSPCRFRKVKIG"
BASE COUNT          381 a          470 c          387 g          466 t
ORIGIN      
        1 cgctcatggt agggattttc tatcaaggtg atgttatttc ccactatctg ctggccgatt
       61 ttgccgctca tgaatgaccg ctgtgagatg aatgtcttgc agccgaaacc caggaaagaa
      121 aagagcagta ccatctgccc caccgcggac ggctcgagga tcaccgtata cggcccggat
      181 tcgagtttaa tcggatttac cgagttcact gccttgtcag cggccttctc cgccagagcg
      241 gttggatcga tcttctcaac atcgcgattg tagccgatgg catagcctga gttttcatta
      301 tcgcccgaga gcgtgagcga aagttccgct ttggttccct caaaaaattg ttccgtcccc
      361 agggaattta aaactgccgc acggtcagtg gtgattctgt atgctccgga tgacagcaga
      421 ttattctttt gaccgacgtc gttgattttt ccaatcgctt cggcacgatt tacggggctg
      481 taattcgcgg tcgattcgta atatgctttg accggaagtg caggtgggga ttgcggtaag
      541 gagacaaact gctcatccgg agtctgcaca gaacttatct cccgcgcagc cttgagcgcc
      601 cgctttatac tttccaaatc aaggctgttg gacaccgaga cgccaattct gtttccctct
      661 atcgtgcgta ctaccacctt gttatctgcg gtcgtcaaat tctgggtgat tttgctgtcg
      721 gcaaaccgtg tgagcgaaag cttctccgac tccagcacaa tctcggtctg atcggcttcc
      781 gaaaattcca gagccttctt gaaagttgag agtatataat cccggctcaa tatttctaat
      841 ttttccggca taaacttatc cgatcttcac tttcctgaat ctgcagggtg aacatccctg
      901 cgcagttctc atattctggc cgggctgacc tttgccgcaa ttgggcgtgc cccataaaac
      961 ccagctatcg gagttcccca ccgcatcaca ggagttccag aagtccaccg tgtttcccga
     1021 gtatcttggc gacttaacca tatcgcccag tctgccgttc tttatttccc agccgacttc
     1081 acctcccaat gtgaagccct cacggttgtc gtcgattgac cagctattgg gagatatcac
     1141 gtaaataccg tcatcaatgc ccgatatcat ctcatccaat gtgctctctc ccggcagtag
     1201 cagaatattc ggaatcctga caatcggcgt attcatccag ccttcggcac gggcgcaggc
     1261 ggttgatttt tttcctattc tatgtgcagt ctccctgtct gagagataat tgaccagcac
     1321 acctttatca atcagtatat tacgctcggt cttcacacct tcgtcatcgt aggcataggt
     1381 tgccagcccg ccgggatagg ttgaatccgc cacaaatgta agcatatctg aagcatattt
     1441 cagactgccc agcttatcgg ttgtggcaaa agatactccg gagaaattgc gctcatagcc
     1501 gaagaccctg tcgagttcca gcggatgccc gaccgattca tgaatctgga gcgaaaccag
     1561 gtctcctccc agtatcaaat ccagctcttt ctgcggacat tcctttgcgc tcagaagctg
     1621 aatcgcctcc tgcgcgatcc tgggcagttc attcttgaaa tcgaacatat ttattatctc
     1681 gtatccaccg gtaatccatt gacc
//
LOCUS       DSEB01000125            3648 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002671, whole genome shotgun sequence.
ACCESSION   DSEB01000125 DSEB01000000
VERSION     DSEB01000125.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002671"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..522)
                     /locus_tag="ENO07_02370"
     CDS             complement(<1..522)
                     /locus_tag="ENO07_02370"
                     /inference="COORDINATES: protein motif:HMM:PF13432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDS00841.1"
                     /translation="MADKMHTNSSNKSEGARGRSSLQILVAGAAIIVLVLLVKFVFLG
                     DKGPANAANGAIDLLKQERFSEAEEKLQKAFANDPELMFASELKEPNLFYVGLANAYL
                     ENGDLNTASRYYTEGYQIDSSGFDKYAKFVRADYHYPEDAYLELGNLFWNAGSLDLAS
                     KAYERALDLNPNFS"
     gene            complement(628..2016)
                     /locus_tag="ENO07_02375"
     CDS             complement(628..2016)
                     /locus_tag="ENO07_02375"
                     /EC_number="4.1.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phenol-lyase"
                     /protein_id="HDS00842.1"
                     /translation="MESGKKRSWAEPFKIKMVEPLKMTTREYRQKCMEDAGYNTFLLK
                     SEDVYIDLLTDSGTSAMSDRQWAGLMLGDEAYAGSRNFYHLEDAVREFYSYKHVVPTH
                     QGRGAEHILSRIMIKDGDYVPGNMYFTTTRLHQELAGGKFVDVIIDEAHDPNSRHPFK
                     GDVDFEKLEDLRKKVGREKIPYVCIATCVNMAGGQPISMANLKKLREWSKEHDIPVIH
                     DATRVAENAYFIKVREEGYKDKSIAEIVYEICSLTDGCTMSAKKDPMVNIGGFLAVND
                     EELFNKARNMVVIYEGLHTYGGMAGRDMEALAIGIEESVQFDHIRARVGQVEYLGQKL
                     LDEKIPIVVPVGGHAVFLNAREFLPHIDQDQFPAQSLAAEIFVESGVRSMERGNVSKG
                     RNPETGENYRPALELVRLTIPRRVYTQAHMDVTAEGIIDVYQKRDKIKGLKFVYEPAY
                     LRFFQARFEKIG"
     gene            2137..2571
                     /locus_tag="ENO07_02380"
     CDS             2137..2571
                     /locus_tag="ENO07_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDS00843.1"
                     /translation="MFSSKTYLALLVVLELSEKERSSPLTSRHLARQYNVSKRYLEVV
                     FNNLAKAGIISSKRGIGGGYFLASDIEKLSIHDLATAAEGGIKIFAGGEYLDEEGDRS
                     ELIKGINGFWNDLNDEMMQNLKRHKLSDLVSYLTDLKEMYYI"
     gene            2588..3520
                     /gene="cysK"
                     /locus_tag="ENO07_02385"
     CDS             2588..3520
                     /gene="cysK"
                     /locus_tag="ENO07_02385"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDS00844.1"
                     /translation="MRIYDSMLELIGGTPLVRLNRITEGLKADVVGKLEFYNPCASVK
                     DRIGYAMLSAAEREGLLKPGGTVVEPTSGNTGIGLAFVCAVKGHPLILTMPDTMSIER
                     RALLKAFGAKIILTPGPDGMPGAVARAEEILAENPDYFMPQQFKNPANPEIHRKTTAE
                     EIWNDTDGRVDILCSGVGTGGTITGVSDVIKSRKPEFKAVAIEPADSPFLSKGEKGPH
                     PIQGIGAGFKPDILRMDLVDEIITIENDEAIDMTRRLVQEEGILSGISSGAIVAGALK
                     VAARPENEGKLIVAIICDTGERYLSTPTYMELGE"
BASE COUNT          906 a          938 c          860 g          944 t
ORIGIN      
        1 gctgaaatta gggttgagat cgagcgcccg ttcataagcc ttactggcaa gatccaggct
       61 tccggcattc cagaacaaat tacccagctc aagataggca tcctcgggat aatgataatc
      121 ggcgcgcacg aacttggcat atttatcaaa cccgcttgag tcgatttgat atccctcggt
      181 gtagtaccgg gaagcggtat taagatcgcc gttctcgagg taagcatttg ccagtcctac
      241 ataaaacagg tttggctctt tcagttccga tgcgaacatc agttcgggat cgttagcaaa
      301 tgccttctgc aacttctctt cagcctccga aaaacgctcc tgcttcaaga ggtctattgc
      361 gccatttgcg gcatttgcgg gtcctttgtc tcccaggaag acgaatttta ccagcagtac
      421 caaaactata atcgcagctc ctgcgacaag tatctgcagg ctgctcctgc ccctggcgcc
      481 ctcgcttttg ttacttgaat ttgtgtgcat cttatcagcc ataaattata tcacctctgg
      541 agatgttata caaaattaac ttgcgggggt ttaaatcccc cgcaattgcc actttatata
      601 tacgaaaact gtggtgattt caagaaatta tccgatcttt tcgaaacgag cctggaaaaa
      661 cctcaaatat gccggttcat acacaaattt caatcccttt attttatccc gcttctgata
      721 gacatcgata atgccctccg cagtcacatc catatgcgcc tgggtataga cgcgcctcgg
      781 tattgtcagc ctgacaagct ccagggctgg acggtaattc tcgccggttt cgggatttcg
      841 gcccttggaa acattgccgc gctccatcga cctgaccccc gattcaacaa agatctccgc
      901 cgccaacgac tgggccggga attgatcctg atctatatgc ggcaggaatt cgcgggcatt
      961 cagaaaaacc gcatgacctc ccaccggcac aacgatcgga atcttctcgt ccagaagctt
     1021 ctgccccagg tattccacct ggcccaccct ggcacggata tggtcgaatt gaaccgattc
     1081 ctctatgcca atcgccagcg cctccatatc acgcccggcc atccctccat aagtatgcaa
     1141 cccctcataa atcaccacca tattgcgagc tttattgaag agctcctcgt cgttgacagc
     1201 cagaaatccc ccgatattga ccatcgggtc cttcttggcc gacatcgtgc agccatcggt
     1261 caagctgcaa atctcataga ctatctctgc gatcgattta tctttatagc cctcttcacg
     1321 taccttgatg aagtatgcat tttcagcgac acgggtggcg tcatgaatga ccgggatgtc
     1381 atgctccttt gaccattccc gaagcttctt taaattcgcc atcgatattg gctgcccgcc
     1441 ggccatattg acacaggttg ctatgcatac atagggaatc ttctctctcc caaccttctt
     1501 gcgtaaatcc tcgagtttct cgaaatccac gtcccccttg aatggatgcc tgctgtttgg
     1561 atcatgcgcc tcatcgataa tcacatccac aaatttgccg cccgcaagct cctggtgcag
     1621 ccgcgtggtg gtgaaataca tattgccggg tacataatct ccatctttta tcatgattct
     1681 ggagaggata tgttcggcgc cgcgtccctg atgagtggga accacatgct tatagctgta
     1741 aaattcccgg accgcatcct ccaggtgata gaaattgcgg ctgcccgcat aggcctcatc
     1801 ccccagcatg agtccggccc attgccgatc gctcatagcc gatgtgcccg aatcggttag
     1861 caggtcgata tagacatctt ccgattttag aaggaatgtg ttatagccag catcctccat
     1921 acatttctgc cggtattcgc gagttgtcat cttcagaggc tcaaccatct taatcttgaa
     1981 tggttcagcc cagcttctct tttttccgga ttccattctc tcccctgact ttaatgttga
     2041 cttttcaata taatcataat aattttatca aaatcctgac aattttcgta ttaatttata
     2101 gataaatcac tcttgcaaca taggatttag gtatttatgt ttagttcaaa gacttactta
     2161 gccttgctgg ttgttttgga actttccgaa aaggagcgtt cctctccctt gacatccaga
     2221 catctggctc ggcaatataa tgtttccaag cgatatttgg aagtagtttt taacaatctg
     2281 gccaaagccg ggattatttc ttccaagcgg gggatcggag gcgggtattt tctggcttcg
     2341 gatatagaaa aacttagtat acatgatctg gcgacggctg ctgaaggggg aatcaagata
     2401 tttgcgggcg gtgaatatct cgatgaggaa ggcgaccgga gcgagttgat caaaggtata
     2461 aatggattct ggaacgatct taatgatgaa atgatgcaga acctgaaaag acataaattg
     2521 tccgatctgg tctcttatct gactgacctc aaagaaatgt actatatctg attccgggag
     2581 gattttgatg agaatctacg attccatgct tgaacttatc gggggcactc cgcttgtaag
     2641 gctcaacaga ataaccgagg ggctgaaagc cgatgttgtg ggcaaactcg aattttataa
     2701 tccctgtgct tcagtgaagg accgtatcgg ctatgccatg ctttccgcgg ccgagagaga
     2761 ggggctgttg aagccaggag gtactgtggt agaaccgacc tccggcaaca cggggattgg
     2821 cttggccttc gtatgtgcgg tcaaaggcca cccgctgatt ttaaccatgc ccgataccat
     2881 gagtattgag cggcgcgccc tcctgaaagc cttcggtgca aagattatcc tcacacccgg
     2941 gccggatggt atgcccggcg ctgtagccag ggcagaggaa atattggcgg agaatccgga
     3001 ttatttcatg ccccagcagt tcaagaatcc cgccaatccg gaaatccacc gcaagactac
     3061 tgccgaggaa atctggaatg acactgacgg cagggtcgat atcctctgct ctggagttgg
     3121 aaccggggga acgattaccg gcgtcagtga tgtgatcaag tcgcgcaaac cggaattcaa
     3181 ggcggtagcg atcgaaccgg ccgattcacc cttcctgtcg aaaggggaga aaggcccgca
     3241 tccgatccag ggcattggag caggattcaa accggacatt cttcgcatgg accttgtgga
     3301 cgaaatcata accatcgaaa atgacgaggc tatcgatatg acccggcggc tggttcagga
     3361 agaggggata ctcagcggca tatcctcggg cgctattgta gcaggcgcat tgaaagtggc
     3421 ggcacggccc gaaaacgagg gcaagctgat cgtggcgatc atatgcgata ccggcgaacg
     3481 ctatctttcc acacccacct atatggaact gggtgaatag tccctcgaac ttgaaaaggg
     3541 atttgaacgg aagaagacgt ggatcaccgt gctcgtgatt aggctcgaat aaatcccctg
     3601 agtgagcggc aactttatgc ttgccctaat gtatatgatt ccttatta
//
LOCUS       DSEB01000126            3583 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002779, whole genome shotgun sequence.
ACCESSION   DSEB01000126 DSEB01000000
VERSION     DSEB01000126.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002779"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..578)
                     /locus_tag="ENO07_02390"
     CDS             complement(<1..578)
                     /locus_tag="ENO07_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00845.1"
                     /translation="MAANHHTRQFDAWNIGSAGLVSYQATYTLQYSTDPAGIAIDAVT
                     SDSIPIMFISSEFSGGVEIVNPVTLEYIGVSTGPSNLAGLDVDDVDDIVYALRRQTRE
                     LYIYQWDPVSQTMTQLAVVLLPGMSWGYGIALNDSLDVLWVTDTPNGMVRAYDVNVTA
                     WSDISEVPSLSFSVPHSPVDITVDGRRNLVFTV"
     gene            complement(1525..2571)
                     /locus_tag="ENO07_02395"
     CDS             complement(1525..2571)
                     /locus_tag="ENO07_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDS00846.1"
                     /translation="MKYKLLGNSGLRVSELCLGAMTFGEEFGWGASKKESKKIFDAFA
                     EAGGNFIDTANKYTLGTSEKYCGEFIYQQREHFVLATKYTLTMKEDDPNFCGNHRKNM
                     IQSVEASLKRLNTEYIDLLWLHAWDYMTPQEEIMRALDDLVRMGKVFYIGISDTPAWI
                     VSRANMLAELRGWTPFVGLQIKYSLLERTVERELLPMARALDLAVTPWGVIGGGVLTG
                     KYKKSGNKIEAEDSERVKTRETVPDRDLEIAAKVQEIAKKRGVSAAQVAINWARQQKG
                     VIIPIIGARKEEQIKDNLKCLEFELNQDELDALDRVSKVDLGFPHEFLHSESIIKVLF
                     GETFDQTENHRSFL"
     gene            complement(2696..3139)
                     /locus_tag="ENO07_02400"
     CDS             complement(2696..3139)
                     /locus_tag="ENO07_02400"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HDS00847.1"
                     /translation="MLSGYICGGSMPFFAWQDKYDIKISSLDEQHRGIAGMINNLYDS
                     LERGVYTDENVGSVLIPIVECLKYHFQTEEKMMMESNYEEYHYHKSTHDRLTDEVMLS
                     VIKIKKNEQVNAREILVVIRDWLLNHIEEEDRKLTAVLNQSPQAK"
BASE COUNT          931 a          886 c          808 g          958 t
ORIGIN      
        1 acagtaaata ctagatttct acggccatca acagtgatat caacgggaga atggggcaca
       61 ctgaatgaaa gggatggaac ctcagaaatg tcactccaag cagtgacgtt gacatcatat
      121 gcgcgaacca tgccgttggg agtatcagta acccatagta cgtctaggga atcgttcaac
      181 gcaatcccat agccccagga cataccgggc agcaatacca cagccagttg ggtcatggtc
      241 tgacttactg gatcccactg gtaaatatag agttcacgtg tttgcctcct caaggcatag
      301 acaatatcat ccacgtcgtc aacatcaagc ccggcaagat tcgagggacc ggtcgacacg
      361 cctatatatt cgagagtaac tggatttaca atttcaacac caccgctaaa ttcgctggag
      421 atgaacatga ttggaatact atcagaagtg acagcatcga ttgcaattcc cgccgggtca
      481 gttgagtatt gaagggtata tgttgcctga tagctgacta atccagcaga acctatattc
      541 caggcgtcaa attgacgggt gtggtgattg gctgccacat acacagattt ggccgccact
      601 attccaacca ttgcgattat aagaagacac aatatgggga gaactagtcc tcgttttcct
      661 tggcgcattt gatacataat caaacctcct gaaaagttaa gtgtgatcgg taatggggtg
      721 agtctttgaa taacgatttt tatattttga tatcaccccc cttcggccat cccgggatca
      781 gtataatcat tagtcccgat tcttcccgga taattttttt gatttcaatg tattaaccaa
      841 tttggacacg atacagtact cccctgacaa aatcgcttcc agctatggga aataatcgat
      901 ttatcctctg cccattcccc cgtgaataat tgctaagtaa tccatttcca cggcaagtaa
      961 aaacgctcgg gtaggcatgc ggtcacttct gccgtcgttg ccccacatcg aaattaggac
     1021 taaatcatgg actttttttg aggctttatg aacactacca aagtaaaccc cacttgaaaa
     1081 atttacatga tcgaaagtcg caagtcaagt cctttttgaa taaaatgaat tttcaagaaa
     1141 aattatgtta atggtttgaa cgtcgaactc aagtacttta gcaaaacaat aagctttccg
     1201 tttgtgcagc attgtttatt cacagcgcgt tctatcaaat ttattgccag ctattgaggg
     1261 atgggcggcg cgtcaaacat catgacgctg aggcccgcga cattttccca tcaaggatcg
     1321 gtgcttgtca aaacaggact cgcgagatgt aagtgcacaa aagtcaacca agacaccaag
     1381 agcacccaga ggtccggcca ttgaccggtt tgggtgcgcc agccaaacta atggaacttg
     1441 gagtatggaa aggggcgtct caggaccggg cgcctgaccg gtcgcgcata gagtattctc
     1501 tgcatatttc ctggcaataa tcaattacaa aaagctgcgg tggttttcgg tctgatcgaa
     1561 ggtttcaccg aagagtacct ttataatcga ttcactgtgc aggaattcat gcgggaagcc
     1621 cagatcgacc ttgctgaccc tgtccagagc gtcaagctcg tcctggttta actcgaactc
     1681 gaggcatttc aggttgtctt tgatctgctc ctcttttcgg gccccgatga ttggaataat
     1741 cacgcccttc tgctggcggg cccagttgat cgccacctga gcagcggata cgccgcgctt
     1801 ctttgctatc tcctgcactt tggcggcaat ttcgagatca cggtccggga cagtttcacg
     1861 tgtcttcacc cgctccgaat cctcagcctc gattttatta cctgattttt tatatttgcc
     1921 ggtcaggact ccgccgccga tcactcccca cggtgtgaca gccagatcca atgcacgcgc
     1981 catgggcaga agttcacgtt ccactgtgcg ctccagcagg ctgtatttga tctgcaaacc
     2041 cacaaaagga gtccatccgc gtaactccgc cagcatattg gcgcgcgaaa caatccaggc
     2101 cggagtgtcg gaaataccga tataaaagac cttacccatg cgcaccagat catcaagcgc
     2161 acgcatgatc tcctcctggg gggtcatata atcccaggca tgaagccaca aaagatcgat
     2221 atattcagtg ttcaggcgtt tcaggctggc ctcgaccgac tggatcatgt ttttgcgatg
     2281 gtttccgcag aagttggggt catcctcttt catggtgagg gtgtatttgg tcgcaagtac
     2341 gaaatgctcg cgctgctgat agatgaattc accgcaatat ttttcgctgg tgccgagtgt
     2401 gtatttgttg gcggtatcta taaagtttcc ccccgcctca gcaaaggcgt caaagatctt
     2461 cttgctttcc ttcttcgagg ctccccagcc gaattcctcg ccgaaggtca tcgcgcccag
     2521 gcaaagttcg gatacccgca gtccgctatt accgagtaat ttgtacttca tagctggctc
     2581 cttttagttc tgtttatttt tatataagcc ggggagaagg ggcggtgttc cgaagagcaa
     2641 ataaaaaaac ccgctctaaa acaaagcggg tttaaatctg tgcggggaaa taaatctatt
     2701 ttgcctgggg gctttgattc aggaccgcgg tcaattttcg atcctcttcc tcgatatgat
     2761 tcagaagcca gtcgcgtatc accaccagaa tttcgcgggc gttcacctgc tcgttcttct
     2821 tgatttttat taccgacagc atgacctcgt cggtcagacg gtcatgcgtg gatttatgat
     2881 aatgatattc ctcgtaattg gattccatca tcatcttctc ttcggtctgg aaatgatatt
     2941 tgaggcattc cacaatcgga atcagcaccg aaccgacatt ctcgtcagta tatacgccgc
     3001 gctcgaggga gtcatataaa ttattgatca tccccgcaat tccacgatgc tgttcatcga
     3061 ggctcgaaat tttaatgtca tatttgtcct gccatgcgaa aaatggcata ctgcctccac
     3121 aaatatagcc agaaagcatg tgtccaggtg ccgaatctgg ttgttatatt ctttttatcg
     3181 tcagggggta accgtatatt gagcaaaaat ttgatataat ttgcctgtat ttgtcgagaa
     3241 gtccacaaca aataagacaa tataaatctc atttatcaat agctcttcaa cgacactggc
     3301 gaatcgcatt cggctcctca atgtaatcta ctgagaatgg ggatcttaat atgtgcgccg
     3361 ggtttctcaa actggcgcca ctgtcgtcgg attagataac ccgccagcat aatagcagca
     3421 tatacagaaa gcctgcccgt aattctgggc aggctccata ttttaattgc atgagattct
     3481 tcggccccac agcccgggac acagaatgat atttgccgat aacctatccg gcgcggtgag
     3541 tcatctcgtc ggcgatgatc tggtcgccga gaccctccac gac
//
LOCUS       DSEB01000127            3464 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002996, whole genome shotgun sequence.
ACCESSION   DSEB01000127 DSEB01000000
VERSION     DSEB01000127.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002996"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            150..2204
                     /locus_tag="ENO07_02405"
     CDS             150..2204
                     /locus_tag="ENO07_02405"
                     /inference="COORDINATES: protein motif:HMM:PF00112.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00848.1"
                     /translation="MVSFFRRVDVPANGIAMLIKVNSAKRKVSPFGLLSALTMFLLLV
                     FASPSQAQLNDEEIEVMQARAESEGWTFEMAKNPATEYSLDQLCGLVEPENWRENARF
                     DPCTPTRNLPVRFDWRDSANLPPARNQGGCGSCWAFGTVGPLECNIAIKDEITVDLSE
                     QYLVSCNSNGWGCQGGWFAHSYHQFVPDPCDSAGAVYESEFPYVAYNAPCNCPYEHSY
                     KIDSWSYIGGNYSVPSVDAMKQAIIDHGPISVAVTVNSEFQAYSGGIFDVPWLTSINH
                     AVVLVGWDDNQGDSGVWFMRNSWGPWWGENGYMRIAYGCCDIGYGACYVVYNKSSRVL
                     FEYETEIPLCINPVEQDSLRLNVTGILGGSVVPGSGLFHYSIEGSAFQAVPMTEYYSN
                     HYRVDFPDIPAGQFIEYYVSVMEENQGRVFDPDTMEAPFKSISGYEELTVFSDNFEQE
                     LSWSVTGDAADGHWSRGVPVNYDRGDPPTDFDGSGKCYLTDNVAGNSDVDDGTATLIS
                     PAFDLSNGNGYVSYARWYSNHAGDDPYNDEMHIYISGDDGATWSLVETAGPQEEASGG
                     WFERGFWADDFVELSDKMKIRFDASDLNDESVVEAAVDAVKISAFALEPPYICGDASG
                     DGLVNVSDAVFIINYIFISGSAEPEPLESAEVNCDGSVNVSDAVLIINYIFTGGTAPC
                     DC"
     gene            complement(2537..>3464)
                     /locus_tag="ENO07_02410"
     CDS             complement(2537..>3464)
                     /locus_tag="ENO07_02410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HDS00849.1"
                     /translation="LDAGFNTIPGTVPQIPPAGSRRGPVGYLLPSDEYRIELTGYQAP
                     LALVTADFDSVKYAFGREPAASSERDDLLVRDGLWAMNHDDIAHIIFMISLQPVDTCQ
                     RFLRVADLSLGPGDSIRIKAETTGEVFVDNCGPQTWYSFDLWYASHTVGYIAGSQLIP
                     LDENRSHIISPQWDNPGQTEVKVYIDIGSDGVIDDSMDVPVEIVTSADDPGESSLPYR
                     FELNQNYPNPFNPTTTIDYSIPERSHVNIDVYNVLGQKIRTMVDREESAGSYTITWDG
                     TNSDGSAVATGIYLYRFQAGDYVETKKMLLMK"
BASE COUNT          797 a          820 c          941 g          906 t
ORIGIN      
        1 aatgtcagcg atgcggtctg gattgtgaat tacgtctttg tcgctggata cgccccgtgc
       61 gatacagatg gcgacggtga gccggactgc tgaacgtgat tgaagtcaaa tattgacaaa
      121 tttatataac aaggtatatt tcagtcagaa tggtttcttt tttcaggcgt gtcgatgtac
      181 ccgcgaatgg aatagcaatg cttattaaag tgaattcggc taaacgaaaa gtatctcctt
      241 tcggacttct ctccgcgctc acaatgtttt tgcttctggt ctttgccagt ccatctcagg
      301 cgcagcttaa cgatgaagaa atcgaggtga tgcaggccag ggccgaaagt gagggctgga
      361 ctttcgagat ggcgaaaaat cctgccacgg agtattctct cgaccagctg tgcggattgg
      421 ttgaacccga gaactggcgt gagaacgccc gctttgatcc gtgcacgccc acacggaatc
      481 ttcctgtccg cttcgactgg cgcgactctg ccaatttgcc cccggccagg aatcagggcg
      541 gatgcggaag ctgctgggct ttcggcactg tcggccctct ggagtgtaat attgcgataa
      601 aggatgagat aacagttgat ctctccgagc agtacttggt aagctgcaac agcaacggtt
      661 ggggatgcca ggggggatgg tttgcgcata gttatcacca gtttgtaccc gacccctgcg
      721 acagcgcggg tgcggtgtac gaatcggaat ttccctatgt agcctataat gctccctgta
      781 actgccctta cgagcactca tataaaatcg attcctggtc atatatcggc gggaactatt
      841 cagtaccgtc tgtcgatgcc atgaaacagg caatcatcga tcacggcccg atttcggttg
      901 cagttacggt caactcggaa tttcaggcct acagcggagg catatttgat gtgccctggc
      961 tcaccagtat aaatcatgcc gtagtcctgg ttggatggga tgataaccag ggtgacagcg
     1021 gtgtgtggtt catgagaaat tcctggggtc catggtgggg cgagaacggc tatatgcgga
     1081 ttgcttacgg ctgttgtgat atcggttatg gtgcctgtta cgtggtttac aataaatctt
     1141 ccagagtcct cttcgaatat gagaccgaga tcccgctctg tataaatcct gttgagcagg
     1201 acagcctgcg gttgaatgtc accggaatcc tgggcgggtc ggtagtaccg ggctcgggtt
     1261 tgtttcacta ttcgattgaa ggtagcgctt ttcaggcggt gcccatgacc gagtattata
     1321 gcaaccacta ccgggtcgat tttcccgata tccccgccgg ccagtttata gagtattatg
     1381 tcagcgtgat ggaggaaaac cagggaaggg tttttgatcc ggataccatg gaagcgcctt
     1441 tcaaatctat ctccggttat gaggaattaa cggtgttctc tgataatttc gaacaggaac
     1501 ttagctggtc agttaccggt gatgccgccg acggccattg gagccgggga gtgccggtta
     1561 attatgacag gggtgatccg cccaccgatt ttgacggttc cggcaaatgc tatctgactg
     1621 acaatgtggc cgggaattcc gatgttgatg atggtactgc cacactgatc tcgcctgctt
     1681 ttgacctgtc gaacggcaac ggttatgtca gctatgcccg ctggtattcc aatcatgccg
     1741 gagatgatcc ttacaatgat gagatgcata tttatatctc cggcgatgac ggcgctacat
     1801 ggtctctggt agaaactgcg ggcccgcagg aagaagcctc ggggggctgg ttcgaacgtg
     1861 gtttctgggc tgatgatttt gttgagctct ctgataaaat gaaaatccgt tttgatgctt
     1921 ccgatctcaa cgatgagtct gtggtcgagg cggctgtgga tgctgtcaaa ataagtgctt
     1981 tcgcgctcga gccgccttat atttgcggtg atgccagcgg ggatgggctg gtcaatgtca
     2041 gcgacgcggt ctttattata aattatattt tcatttctgg atcagcggag cctgagccat
     2101 tggaatcggc cgaggtaaac tgcgacggca gtgtgaatgt gtctgatgca gttcttataa
     2161 taaattacat cttcacaggg ggcacagctc cctgcgactg ctgaagtaaa tcctggaatc
     2221 aaaatatact gtcaaatcct gcccggaaat ccgggagggg caacagtgtt tatgtgagaa
     2281 tggactatat ctaaaaacaa tgcaaaccca ccctggcgaa gcttactgca aatgtggact
     2341 atttggaaaa aatcaggatg ctgtctctct gagagagcaa agcgagcgca aaatctcatg
     2401 ttagcttatg agattcttcg cccggataaa tcggtactca ggatggcgga ctcctgatta
     2461 tcctttttca gtactcctga cgggttgggt catcccgcca atactgtatg gagttgtatc
     2521 ctgaccatct ttcgttctat ttcatcagca acatcttttt cgtctcaaca tagtctccgg
     2581 cctggaagcg gtagagatag atacctgtgg caactgctga accatccgaa ttagtgccgt
     2641 cccatgtaat ggtatatgaa cctgccgact cctctcgatc gaccattgtg cggattttct
     2701 gcccgagcac gttgtaaaca tcaatgttga cgtgactgcg ttctggaata ctgtaatcga
     2761 ttgtagtggt cggattgaac ggattgggat agttctggtt caattcgaac cggtagggca
     2821 agctcgactc acccggatca tcggcagacg tcacgatttc gaccgggaca tccatggagt
     2881 cgtctatcac cccatcggat cctatgtcga tgtacacttt gacctcagtc tgtccgggat
     2941 tatcccactg cggagatata atgtggctcc tgttttcgtc cagcgggatc aactggcttc
     3001 cggctatgta tccgactgta tgagatgcat accagaggtc gaagctgtac caggtttgcg
     3061 gtccgcagtt gtctacaaac acctcgcccg tggtttcggc cttgatgcgg atggaatccc
     3121 cgggtcccag cgataagtca gcgacacgca aaaagcgctg gcaggtatcc acgggctgca
     3181 gagagatcat aaaaataatg tgcgcaatgt catcatgatt catggcccat aagccgtccc
     3241 gtacaaggag atcatcccgc tccgaggagg ctgccggttc gcggccgaag gcgtatttaa
     3301 ccgaatcaaa atcggctgtc acaagcgcca acggtgcctg gtacccggtt agttcaattc
     3361 tgtattcgtc ggatggaagc agataaccga ctggccctct cctgctgcca gcgggcggta
     3421 tctgcggcac tgttccggga atggtattga atcctgcgtc gagt
//
LOCUS       DSEB01000128            3588 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002774, whole genome shotgun sequence.
ACCESSION   DSEB01000128 DSEB01000000
VERSION     DSEB01000128.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002774"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..544
                     /locus_tag="ENO07_02415"
     CDS             <1..544
                     /locus_tag="ENO07_02415"
                     /inference="COORDINATES: protein motif:HMM:PF05193.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDS00850.1"
                     /translation="FIGYKVVEAGHPDVYPLRVMGKILFQGESSRAYKRLVDEEQLCL
                     YAGGDMFEFEDPGLFYLIAVMKSPDKSTEEAEGILYEEIEKLKTEPVPQEILQKALNQ
                     IEAEKWYQLESNENKAYAIGWFETIRGDYSLMFDELDNYLAVTADDIMRVAGEYFDET
                     NRTVVTLVPKTSGATPNIGF"
     gene            558..1943
                     /locus_tag="ENO07_02420"
     CDS             558..1943
                     /locus_tag="ENO07_02420"
                     /inference="COORDINATES: protein motif:HMM:PF00675.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDS00851.1"
                     /translation="MKYRSLFIIGLVMLFAFTGSGLTIEMELPEIEHKALDNGLDVYV
                     IEQHEVPVVSMRLVVRGGTLYETEETIGIANLTSSLLRKGTTNRTADEISEEIDFIGG
                     SLGAGAGNDAIYATCKVLSKHLDKGMELLADIMINPIFPEEEIEKQKDQFLGSIMQNK
                     SDPTTIREMQFYRLLFGQHPYGFPMIGTEETIMLLTDSDIKDFYNKYIIPNDAFILVV
                     GDVQPGEAIEKVEKYFGPWKPGTPPTIDIPEPAQIENTRVVLIDKPDATQSYIAMGHL
                     GISRLSPDAFACRVMNYILGGGGFASRLTKEVRGEGGLTYGIQSMYDYNKYRGAFAVK
                     TFTKNESTGEAIDLILEELRRIREEMVTEEELNDTRNFYSGYIPIQFETPDKIASYVE
                     TIYLYDLGEDYYSRYINTVSNTTRQDVQDAAQKYIDPNSMLIVVVGKAEEVKPQLEKY
                     GEVEVIPLIEL"
     gene            2099..2455
                     /locus_tag="ENO07_02425"
     CDS             2099..2455
                     /locus_tag="ENO07_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00852.1"
                     /translation="MNKFGDDLAPLIFTTFAELHRRNALQDAFVFLLMRMFHRKYPSV
                     DHEFEPRKKLSSNEILQYMQFTPEYRKDARRLTGFTEGQMAAIIDDFIQILCRQFLCN
                     PDVYELIENKLKSNRD"
     gene            2525..3274
                     /locus_tag="ENO07_02430"
     CDS             2525..3274
                     /locus_tag="ENO07_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00853.1"
                     /translation="MSEQKAASEKFAQLIDSKYSDYLNVIGDCKDICYYFIDGFSSHP
                     DKEPIIETAIRVQRLTLEYIESILCVLRQGFITQAIVLLRSLFELSNLYTYVYMDEEH
                     FKKWQKEKKIKPNIIRRTLKKQGFDPGQKTYGDLSKFTHANYEFISEHEALNALAPVN
                     DMQKLMIPHCMIDMMRFLMRISLVSCKIYGEHVEKAEDIIRMKNEELDHLRENVLQLD
                     EKQARSEEEYSEFKKLLQRIKTRHFEEEDRT"
     gene            complement(3408..>3588)
                     /locus_tag="ENO07_02435"
     CDS             complement(3408..>3588)
                     /locus_tag="ENO07_02435"
                     /inference="COORDINATES: protein motif:HMM:PF03471.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDS00854.1"
                     /translation="EDKADTIGGLIFTHLGELPDRRQKIRINGIEFTITTLKGNRIEK
                     VLARRIEGPEDQQKI"
BASE COUNT          966 a          825 c          904 g          893 t
ORIGIN      
        1 gttcattggt tataaggtgg tcgaagcggg tcatcctgat gtctatccgc tgcgggtcat
       61 gggcaaaatc cttttccagg gcgaaagctc gcgggcctat aaacgcctgg tggatgagga
      121 gcagctctgt ctctatgccg gcggggatat gttcgaattc gaggatccag gattgttcta
      181 tctcatcgct gtcatgaaaa gccccgacaa gagcaccgag gaggccgaag gaattctata
      241 cgaggaaatt gagaagctca aaactgagcc ggtgccgcaa gagattctgc agaaggccct
      301 gaaccagatc gaggccgaga agtggtatca gcttgaatcg aatgaaaata aagcttatgc
      361 tatcggctgg tttgaaacga tcaggggtga ctacagcctg atgtttgatg aactggacaa
      421 ctatctggct gttaccgctg acgatattat gcgtgtggcc ggtgagtatt tcgacgagac
      481 taaccgtacg gtggtaacgc tggtaccgaa aacatccggc gcaacaccca atatcgggtt
      541 ttaggaaggt gctaaaaatg aaatatagaa gcttgtttat tattggcctg gtaatgctct
      601 ttgcctttac aggctcgggc ttaacaatcg aaatggagct tcccgaaatt gaacataaag
      661 cgctggataa cggcttggat gtttacgtta tagaacagca tgaggtaccg gttgtctcca
      721 tgcggctggt tgttcgcggg ggaaccctgt acgagaccga agaaaccata ggtattgcaa
      781 atttaacttc cagccttctt cgcaaaggca caacgaaccg gaccgccgac gagatctctg
      841 aggaaattga tttcatcggt ggtagccttg gtgcaggggc aggcaatgac gccatctatg
      901 ccacctgtaa agtcctcagc aagcacctgg ataagggtat ggaactgctg gcggatataa
      961 tgatcaatcc catctttccc gaagaggaga tcgaaaagca gaaggaccag ttcctcggtt
     1021 ctatcatgca gaataaatcc gatcctacca ccattcgcga aatgcagttc tacagattgc
     1081 tttttggcca gcacccttat ggctttccga tgatcggaac ggaagagacg ataatgcttc
     1141 tgaccgattc cgatattaag gatttttaca acaagtatat catcccgaat gatgctttta
     1201 ttttagttgt tggcgatgtt cagcctggag aagccatcga aaaagtcgaa aagtacttcg
     1261 gcccctggaa gccgggtaca ccgccgacaa ttgatattcc cgagcccgcc cagatcgaaa
     1321 atacgagggt agttctgatc gacaagccgg atgccacaca gtcttatatc gccatgggcc
     1381 atctcgggat aagccgtctc agtcccgatg cctttgcctg cagggtcatg aactatatcc
     1441 tgggcggggg cggattcgcc tcccgtctga ccaaagaggt cagaggagag ggcggtttaa
     1501 cctacggtat ccagtccatg tacgactata acaaatacag gggagcgttt gcggtaaaga
     1561 catttaccaa gaatgaatcg accggcgagg cgattgacct gatcctcgag gaactgcgcc
     1621 gtatccgcga agagatggtg accgaagaag agctcaatga cacgcgcaat ttctacagcg
     1681 gatatatacc gattcagttc gagactccgg acaaaatcgc cagctatgtg gagactatct
     1741 atctttatga tctgggcgaa gattactaca gccgctatat aaacactgtc agcaatacaa
     1801 cccggcagga tgtgcaggat gctgcccaga aatatattga tcccaacagc atgctgatcg
     1861 tggtcgtcgg caaggccgag gaggttaagc cgcagctcga gaagtatggc gaggttgagg
     1921 taattccttt gattgaatta taattttaaa ctgatcttat ataaaagcct cccgaattca
     1981 atttgggagg ttttttgttt ctttttttta aattattgtt tatcttcttg atatgaaacg
     2041 cgaagaggtg gccgggaata tacttctttg cggactctcc cacggccgag aacatgttat
     2101 gaacaagttc ggcgatgatc tcgccccctt gatatttaca acttttgctg aactgcacag
     2161 acggaacgcg ttgcaggatg cctttgtatt tctgctgatg cgcatgttcc atcgcaagta
     2221 tccctctgtg gatcatgagt ttgagccgag aaagaagctc tcctcgaacg aaattttgca
     2281 gtatatgcaa tttacgccgg agtaccgcaa agatgcgcga cgcttgaccg gtttcacgga
     2341 gggccagatg gccgcaatta tcgatgattt catccagatt ttatgccgcc agttcctctg
     2401 caacccggac gtgtacgagt tgatcgagaa taagttgaag tcaaatcgag actgacactt
     2461 gccagtgacc ttttcgaatc aaatccagat ttgtttccgc caaaaatcgt attatattat
     2521 ggttatgagt gagcagaagg cagcaagcga aaaattcgcg cagttgattg acagcaaata
     2581 tagcgattat ctgaacgtca tcggtgattg taaggatatc tgctattatt ttattgacgg
     2641 tttttcaagc catcccgaca aggagcccat tatcgaaacc gccataaggg ttcagcgcct
     2701 gaccctggag tatattgaaa gtatcctgtg cgtcctgcgt cagggattta tcacccaggc
     2761 gattgtcctg ctccgctcat tgtttgaact cagcaatctc tatacatatg tgtatatgga
     2821 tgaagaacat tttaagaaat ggcagaaaga gaaaaaaata aaaccgaata tcatcaggcg
     2881 cactctcaaa aagcagggct tcgaccccgg ccagaagacc tacggcgacc tgagcaagtt
     2941 cacgcatgcc aattacgaat ttatttccga gcatgaggcc ctgaatgctc ttgccccggt
     3001 caatgatatg cagaagctga tgattcccca ctgcatgatc gatatgatgc gcttcttgat
     3061 gcgcatcagc ctggtttcct gcaagattta cggcgagcat gtcgaaaaag ccgaggatat
     3121 aattcgaatg aaaaacgaag agctggacca tctgagggaa aatgttctcc agttagacga
     3181 aaaacaggca cgttccgagg aagagtacag cgagttcaaa aagctcctcc agcgcatcaa
     3241 aacccgccac tttgaagaag aagatagaac ctgatctgat cgcggcgggc ctccagtggg
     3301 tgcgcgcgtt tgcagcccaa actgctttag ttctcgaaat ttgacaccat gaagcgcctt
     3361 gcattccaga atcccgggag gagttgacgc gctatgccat aaatcgatta aattttctgc
     3421 tgatcctcgg gaccctcaat ccggcgggca agcacttttt cgatgcggtt gcccttcagg
     3481 gtggtgatgg tgaattcgat gccattaatc cggattttct ggcggcggtc gggaagttcc
     3541 cccagatgtg taaagatcag ccctccgatc gtatctgctt tatcctca
//
LOCUS       DSEB01000129            4095 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002073, whole genome shotgun sequence.
ACCESSION   DSEB01000129 DSEB01000000
VERSION     DSEB01000129.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002073"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..447
                     /gene="eno"
                     /locus_tag="ENO07_02440"
     CDS             <1..447
                     /gene="eno"
                     /locus_tag="ENO07_02440"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013244604.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDS00855.1"
                     /translation="IISIEDGLAEDDWDGFIEMTKTLGDKVQIMGDDLFVTNPRRLKQ
                     GIEKKAANSILIKLNQIGSLSETLETIEMAQKNSFTAVVSHRSGETEDTTISDVVVAT
                     QAGQIKTGSLCRTDRIAKYNQLLRIEEELGDTARYAGKTAFYNLNL"
     gene            444..830
                     /locus_tag="ENO07_02445"
     CDS             444..830
                     /locus_tag="ENO07_02445"
                     /inference="COORDINATES: protein motif:HMM:PF04977.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00856.1"
                     /translation="MSKARRKKYQGVDYAPGNLIRRLKLENTKKRRGMIKALVLTVAV
                     AVGVKLAVGPFGTLELIRMDQRKRILEDKIRLLSADLTNLEWQVRQLSNPLYIEKLAR
                     EKYWMVKPGEIIIKLPAESYQQDDPS"
     gene            817..1587
                     /gene="recO"
                     /locus_tag="ENO07_02450"
     CDS             817..1587
                     /gene="recO"
                     /locus_tag="ENO07_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDS00857.1"
                     /translation="MTLHRSDAIILKRDRSGESSLMVEVFLRNKGRSTLLAKGARKPG
                     SSMVGKLEPFSEVELLYYRKNDESLGVISQVEEKRSNSDLSGDIRRLSYASAVVEALK
                     GMVYRGEEGQESMYNLVAATFYMLNYALPRKLEFFFLVFLLKSLDLIGLSPQFEHCVK
                     TGKPIEEEEILFSVELGGVISKSAASSENTYFKLDRGIVNSIKKARESDIDKLKGLVF
                     TEKQSAVIKDLLFSFLSYHTEGKPGFNSLNFLSKLANY"
     gene            1623..2933
                     /locus_tag="ENO07_02455"
     CDS             1623..2933
                     /locus_tag="ENO07_02455"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HDS00858.1"
                     /translation="MEKLVSLCKRRGYIFPSSEIYGGLESCWDYGPLGAELKRNLKDF
                     WWKSMTMKRDDIEGLDASILMHPRVWEASGHLTSFTDPMVDCKTCGTRFRADSLEAET
                     CPMKPSKSPLECGGELTEARNFNLMFKTFMGPVEDESAVIYLRPETAQGIYVNFHNVQ
                     KPSRQKIPFGIAQIGKAFRNEITTKNFIFRTREFEQMEMQYFIHPSEDEKWFEYWRDQ
                     RLKFYHDLGINPEKLHLKEHGPDDLAHYAKRAVDIEYEFPFGIKELEGIHNRTDYDLS
                     HHQETCGKDLRFFDQVRNERYLPYIIETSAGCDRTILVVLLDAYHEEEVKGEKRVVLK
                     INPRLAPVKVGVFPLVKKEGMPEIALDIYHNLKKHYKCFYDDGGAVGRRYRRQDEAGT
                     PYCVTVDGQTKEDNTVTVRERDSMEQIRLKVDDLVAEFRNKLEF"
     gene            2963..3541
                     /locus_tag="ENO07_02460"
     CDS             2963..3541
                     /locus_tag="ENO07_02460"
                     /inference="COORDINATES: protein motif:HMM:PF13505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HDS00859.1"
                     /translation="MIRVLVILLILTGPVFAQGFSYGVHACGSWLKSDFEAGGFSYEP
                     EDYEAIYGLGVDGMFKPMLLPIGVEAGFTYLSSSEIEGETPFKAKGHPLYINGKYYFS
                     PFTYVGAGLNYTFWDVEIEGNDVDVDSKIGFQVGVGAEFGVSSLKLYGSAFYYVQNGK
                     FAVEDFPIAADDDEADVKLKGVQIRAGVRFGG"
     gene            complement(3568..>4095)
                     /locus_tag="ENO07_02465"
     CDS             complement(3568..>4095)
                     /locus_tag="ENO07_02465"
                     /inference="COORDINATES: protein motif:HMM:PF04932.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDS00860.1"
                     /translation="GLLGIFYIMHSQALLLDFARVTRLLGGLSGRTIIWETGLVMIED
                     HPLFGVGPGNVSRLLYDYAQATHPIVSIDLRSAMERGIMHNGYLQQLAEIGIIGLMIA
                     LWIIWRFIKLLFSYVHSKCSQSLTNISIIVLALFIGRLAHSLFESSLDIGPYTVDVSV
                     LLIYAAVVKLHDAKT"
BASE COUNT         1106 a          910 c         1085 g          994 t
ORIGIN      
        1 attatttcca tcgaggatgg tctggccgag gatgactggg atggatttat tgaaatgacc
       61 aaaactcttg gagataaggt gcagataatg ggagatgatt tgttcgtaac caatcccagg
      121 agactcaagc agggcatcga gaagaaggcg gccaactcga ttttgatcaa gctcaaccag
      181 ataggcagcc tttcggaaac tctcgagact attgaaatgg cccagaaaaa cagctttacc
      241 gccgtagtat cccatagaag cggtgaaacc gaggatacga ctatctccga cgtcgtggtt
      301 gcaactcagg ccgggcagat caagaccggc tcgctttgca ggacggacag gatagctaag
      361 tataatcagc tcttaaggat tgaagaggag cttggcgata ccgccagata tgcgggcaaa
      421 accgcatttt acaatttgaa tctatgagca aagccagaag aaaaaaatat cagggtgtgg
      481 attatgcgcc gggaaatctt atccggcggc tgaagctgga aaacacgaaa aagcgccgtg
      541 gcatgattaa ggcgctggtt ttaacagtcg cggtcgccgt gggtgtgaag ctggctgtcg
      601 ggccgttcgg caccctggaa ctgatccgca tggatcagag gaagcgtata ctcgaagata
      661 agatacgcct gctttccgcc gacctgacca acctggaatg gcaggtccgc cagctctcca
      721 acccgttgta tatagaaaaa ctggcccgcg aaaaatactg gatggtgaaa ccgggcgaga
      781 taatcattaa acttccagcc gagtcgtatc agcaagatga cccttcatag atcagacgct
      841 atcattctca aaagagaccg atcaggcgaa tcctccctta tggtcgaggt gtttctaaga
      901 aataagggac gctcgaccct gttggccaag ggcgcccgta aaccgggatc gtcaatggtc
      961 gggaagctgg agccgttcag cgaagtcgag ctgctttact atcgtaagaa cgatgagagc
     1021 cttggagtca tctcgcaggt ggaagagaaa cgctccaaca gcgacctttc cggagatatc
     1081 cgccgattga gctatgcctc ggctgtggtg gaagccttga agggcatggt atatcgcgga
     1141 gaagaggggc aggagtcgat gtacaatctg gttgccgcca cattctatat gctgaattat
     1201 gcactcccca ggaagctgga gttcttcttt ctggttttcc tgctcaagtc tctcgattta
     1261 atcggccttt ctccgcagtt tgagcattgc gttaaaaccg ggaagccgat cgaagaggaa
     1321 gaaattcttt tttctgtcga gctcggcggt gtaatatcga aatctgcggc atcatcagag
     1381 aatacctatt tcaagctcga tcgaggcatt gtgaattcta tcaagaaagc gcgtgaatcc
     1441 gatattgaca aattgaaagg actggttttt accgaaaagc aaagcgcggt cataaaggat
     1501 cttttgtttt catttttgag ttaccatacc gagggcaagc ccggttttaa ttcccttaat
     1561 tttctttcta aacttgcaaa ttattgactg gaggccatgt ggcaagaaat gaatctggtt
     1621 taatggaaaa gcttgtgagc ctgtgtaagc ggcgcggtta tatattccct tcgtccgaaa
     1681 tctatggtgg tttggagtcc tgctgggatt atggcccgct gggcgcggaa ttgaaacgga
     1741 acctgaagga tttctggtgg aaatccatga ccatgaaacg ggatgatata gaaggtctgg
     1801 atgcctcgat actgatgcat cccagggtct gggaagcctc cggacacctg acctcattta
     1861 cggatcctat ggtggactgc aaaacctgcg ggacacgctt tcgggccgac agcctggaag
     1921 ccgagacctg cccgatgaaa ccctcgaaat ctccgcttga atgcggagga gagctgaccg
     1981 aagcccgcaa tttcaacctg atgtttaaga ctttcatggg tccggtcgag gatgaatcgg
     2041 cggtgatata cctcaggccc gagacagccc agggtatcta tgtcaatttc cataatgtcc
     2101 agaaaccgtc ccgtcagaaa ataccttttg gaatcgcaca gatcggaaaa gccttcagaa
     2161 acgagattac cacaaagaat tttatttttc gcacacgcga gttcgagcag atggagatgc
     2221 agtattttat tcatccttct gaagatgaga aatggttcga gtactggagg gatcagcgcc
     2281 tgaaattcta tcatgatctc gggatcaatc ccgaaaagct tcacctcaaa gaacatggcc
     2341 ctgacgatct ggcccattac gccaaacggg cagtcgatat tgaatatgaa ttcccgtttg
     2401 ggatcaagga gctggaaggc attcataacc gtacagatta tgatctatcg catcatcagg
     2461 agacctgcgg caaggatttg cgctttttcg accaggttcg caacgaacgc tatcttcctt
     2521 atataattga gacctcagcc ggctgtgacc gcacaatcct cgtggttctt ctggacgcct
     2581 atcatgaaga ggaagtcaag ggtgaaaagc gggtggtact taaaatcaat ccccgccttg
     2641 cccctgtcaa ggtaggtgtg tttccactgg taaagaaaga ggggatgcct gagatcgccc
     2701 tggatatata ccataatctt aaaaaacact acaaatgttt ctatgacgac ggcggagctg
     2761 tcggacgccg gtaccggcgc caggatgagg ccgggacgcc ctattgcgtc acagttgacg
     2821 gacagacgaa agaagataat acagtaactg tgcgggaacg tgatagtatg gagcaaatca
     2881 ggctcaaggt tgacgatctg gttgccgaat ttagaaataa gctggaattc tagaaaaatc
     2941 ttattatatc gtggaggtca aaatgattag ggtgctggtt attttattga ttttaacagg
     3001 tccggttttt gcgcagggct tttcatacgg cgtacatgcc tgcggatcat ggctcaagag
     3061 cgactttgaa gccgggggct tttcttatga gcccgaggac tatgaggcca tttacggtct
     3121 tggtgtggat ggtatgttta agccgatgct tctgccgatc ggggtcgaag cgggattcac
     3181 ctatctttcc agcagtgaga tcgagggcga gacacccttt aaggccaagg gccaccccct
     3241 gtatatcaat ggcaagtatt attttagccc attcacgtat gtaggcgccg gtctcaatta
     3301 cactttctgg gatgtggaaa ttgagggtaa tgacgttgat gttgacagca aaattggatt
     3361 tcaggttgga gttggtgcgg aattcggcgt cagcagccta aagctttatg gcagcgcatt
     3421 ttattatgtg cagaatggca aatttgcggt agaggatttt ccgattgcgg cggatgatga
     3481 tgaagctgat gttaagctaa agggagtcca gatcagggcc ggagtaagat tcggcggctg
     3541 agcaagagag ttcaaatcaa gctggaatta ggttttggcg tcatgcagct ttacaactgc
     3601 cgcataaatc agaagcaccg aaacatcaac tgtataaggc cctatatcca gcgagctctc
     3661 aaatagcgaa tgggcaagcc tgccgataaa tagagccaac acaataatgc tgatatttgt
     3721 cagactttgg ctgcatttgg aatggacata cgaaaatagc agctttatga atctccagat
     3781 tatccacagg gcaatcatca ggcctattat tccaatttct gcgagctgct gaagatagcc
     3841 gttgtgcata atgccgcgtt ccatagccga tcgcagatcg attgagacga ttggatgggt
     3901 cgcctgggca taatcataga gaagtctgga cacgttgccc ggacctaccc cgaacagggg
     3961 atgatcttca atcatcacca ggcctgtttc ccatataatg gttctgcctg ataatccgcc
     4021 cagcaggcgc gttacgcgcg caaaatccaa caagagggcc tgggagtgca ttatataaaa
     4081 gatgcccaac agacc
//
LOCUS       DSEB01000130            1620 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1016135, whole genome shotgun sequence.
ACCESSION   DSEB01000130 DSEB01000000
VERSION     DSEB01000130.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1016135"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            3..545
                     /locus_tag="ENO07_02470"
     CDS             3..545
                     /locus_tag="ENO07_02470"
                     /inference="COORDINATES: protein motif:HMM:PF16653.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00861.1"
                     /translation="MEELSFPSPFEKLEAFQTSGGTSTLPLTYYGKVKNLDYKTIRYP
                     GHSRLFQTLISLGFCSSETVLVNENKVVPRHLTGMLLERALGRDEKDVVLVSVDFEGR
                     KDGKNRHLKYRIIDYYDESTGLTAMMRSTGFSSSIIAQMLAEGKIERKGVVPQEISVP
                     PDVFVEELSRRNFSLEEIWE"
     gene            703..>1620
                     /locus_tag="ENO07_02475"
     CDS             703..>1620
                     /locus_tag="ENO07_02475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDS00862.1"
                     /translation="MAKKLTAGFIIVVLVGAIIFFGFQSDSGEANEGIKLFPVERGHI
                     VDKAIAIGEIEPEKEIAVKSHISGVVQKLFVEENDQVDVGDALFEVSPRSTPEQIVNA
                     ELSLQETRVEFENAESEFERVKELFNKNLLSESEYDRSKKTLEMARLDVQRKQEALSL
                     LKEGKTMLTEEAIESVIRAPVAGTVLAIYGEEGDPVEPMTSFQPGTPIMSLADMSNLV
                     FRGTVDEIDVGKLSEGMDVTLKIGALPSDTVRGRLYKIASKARKQDQTTVFDVEIEIT
                     EAGPNLLRAGYSATAEIIINEKNDVLMVPE"
BASE COUNT          440 a          362 c          449 g          369 t
ORIGIN      
        1 aggtggagga gctctcgttc ccctcgccct ttgaaaagct ggaggccttc cagacctcgg
       61 gaggcacctc aaccctgccg ctcacttatt atggcaaagt gaagaacctg gattataaga
      121 ccatccgcta tcccgggcac agcaggctct ttcagaccct tatttcgctt ggattttgtt
      181 catccgagac ggtgctggtg aacgaaaaca aagtggtgcc gcgccacctg acaggaatgc
      241 ttttggaaag ggccctgggc agagatgaga aggatgttgt cctggtctcg gtagactttg
      301 agggcaggaa ggacgggaag aacaggcacc tgaaatacag aatcattgat tattacgatg
      361 aaagcacagg cctcacggct atgatgcgct caaccggatt ctcctcatcg ataatagcac
      421 agatgctggc cgagggtaaa attgagagaa agggtgtagt accccaggag atctcagtac
      481 ctcccgatgt ttttgtggaa gagctctcgc gccgcaactt ttctctggaa gaaatttggg
      541 aatagcatag atatcccttt tcgttttcaa tcaggccatc cccaaggatt aaaatgcaac
      601 aaatacagga caacgcggtc gtatattaac ggaaaccttg atttgaaagc ttcgtattaa
      661 actatgtaat aggtcaataa aactcacata ccggggagaa acatggcaaa aaaattaact
      721 gcaggattta taatagtagt acttgttggg gccataatct ttttcggatt tcagagcgac
      781 tcgggcgagg ccaatgaggg catcaaactt ttcccggttg aaagaggtca tatcgtagat
      841 aaggccatag cgatcggcga gatcgagcct gaaaaggaaa tcgcagtgaa atcccatatt
      901 tcgggagttg tacagaagct ctttgtggaa gagaatgatc aggttgatgt gggtgatgcg
      961 ctcttcgagg tttcgcctcg gtccacgccg gaacagattg tcaatgctga actctcgctt
     1021 caggaaaccc gggttgaatt cgaaaatgcc gaatcggaat ttgagcgggt aaaagaactt
     1081 ttcaataaga atcttctctc ggaatctgaa tatgaccggt cgaagaagac gctggagatg
     1141 gcgcgtctgg atgttcagcg caagcaggag gcgttatcgc ttttgaaaga gggcaaaacg
     1201 atgctgaccg aagaggcgat cgaatcggtc atccgggctc cggtagcggg cacggtgctg
     1261 gcgatttacg gcgaggaggg cgaccctgtc gagccgatga catcattcca gcccggcacg
     1321 ccgattatgt cgctggccga catgagcaac ctggttttca ggggaactgt cgacgaaatc
     1381 gatgtcggca agctgagtga aggtatggat gtgaccttga aaatcggcgc gctgccctcg
     1441 gatacggtac gtggacgtct ttataaaatc gcatccaagg cccgtaagca ggatcagacc
     1501 actgtttttg atgtcgaaat cgaaatcacg gaggccgggc cgaatctgct gcgcgccggg
     1561 tattcggcca cagcggaaat cataatcaac gagaaaaatg atgtgctcat ggtaccggaa
//
LOCUS       DSEB01000131            2578 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1005766, whole genome shotgun sequence.
ACCESSION   DSEB01000131 DSEB01000000
VERSION     DSEB01000131.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1005766"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1414)
                     /locus_tag="ENO07_02480"
     CDS             complement(<1..1414)
                     /locus_tag="ENO07_02480"
                     /inference="COORDINATES: protein motif:HMM:PF13432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDS00863.1"
                     /translation="MIEYSKISGISNQEELDAFIKEFYEGDRDIFVSELNKAVSRILH
                     VNLKEAGRIVKKAAELFLYLPDHYRARLWTIEARYENWCGNPQSALRKYRRAIKKMLA
                     LRQFELAARTRQGLMDVEMYLGKYDDALRAGQKALAYFKRKDNLNLAARVMTNIGNIY
                     HRLDKNRMALRYYDRARGIFVRDGGIPLAIVDYNRANIFVNMGELGKAESLYRETSKI
                     YRHAGITINAVKADYSLAYLYFLTDRYTRALKAFEKVYQDFMDLGDNKSAAAALLDMA
                     EMNIYLHQYSSSIALAEKVIPLYRKFKQPYEEGKAHYFVAQSLSRLGDHKDAGRYLTD
                     ADKLFRSEDNRLWQGMVYYLKSQIDLDKNRTKSAISSARKSREFFAASGDERRSVDAE
                     LVLLEAELSSNNGGLRRAKKIKEQVHLGYQKYNLLKVIGEYYLRRGDYRAASHQFRAA
                     IKIVEKMLLNLYPDEIRFFFT"
     gene            complement(1411..2001)
                     /locus_tag="ENO07_02485"
     CDS             complement(1411..2001)
                     /locus_tag="ENO07_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00864.1"
                     /translation="MSGKNDKYDLLADYLKGRLSGDRKRQFERMLKEDPELSRTAEFV
                     SELIRESDDIKWDRLKQPAHSVLERMLKDYKLSRKSTDAKQGIIFFDSKDMPLPEDVR
                     PDIISTRRVKYRVGDDILEISLYPISPGSYQLIGQLSGLQPGDIIEIEIKGRKSTKKV
                     KSNAFQLFRLERIESGRYNIRIYAEKEVIAKIDIEL"
     gene            complement(2047..>2578)
                     /locus_tag="ENO07_02490"
     CDS             complement(2047..>2578)
                     /locus_tag="ENO07_02490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDS00865.1"
                     /translation="LCSEGDEDAWHELIDIIGPLIFSICRKSRLTHDESFDIYGQVCF
                     DLVGNIGTIKSPARILSFVATITRRKIFNFYEKMRLADYFKLEVVPMLSESQEEPPDL
                     TYENMQRRELLMKAMTKLPTRQYRLLKALFFDPGNPSYKEIARKLQVPASSIGPTRAR
                     ALEKLGRILKRELYKF"
BASE COUNT          637 a          645 c          549 g          747 t
ORIGIN      
        1 gcgtgaagaa aaagcggatc tcatcaggat agagattaag cagcattttc tcaactattt
       61 tgatggccgc ccgaaattga tgggaggcgg ctctataatc cccccgtcga aggtaatact
      121 cgccaatgac cttcaaaaga ttatacttct gatatcctaa atgaacttgc tccttaatct
      181 tttttgctct tctcaagcct ccattgttag agcttaattc agcttccagg agcaccagtt
      241 cagcatcaac acttcttcgc tcatcaccgc ttgctgcgaa aaactctctg gacttgcggg
      301 cggaactgat cgcggacttt gttcgatttt tgtcaagatc gatttggctt ttaagataat
      361 ataccatccc ctgccagagc cggttatcct cgctcctgaa gagcttatct gcgtcggtca
      421 aatatctgcc cgcatctttg tgatctccca gccggctcaa agattgcgcg acaaaataat
      481 gcgcttttcc ttcttcgtag ggctgcttga attttcggta gagcggaatc accttttctg
      541 ccagcgcgat actggaggaa tattgatgca gatagatatt catttcagcc atatccaaaa
      601 gtgcggcagc ggccgacttg ttatcaccca aatccataaa atcctgataa actttctcga
      661 acgctttcag agcgcgggta tatctgtccg tcaagaaata gagataggct agggagtaat
      721 cagctttgac ggcatttatc gttatcccgg catggcggta tatctttgag gtttcgcgat
      781 acagactttc agcttttccg agctctccca tgttaacaaa aatattggca cgattataat
      841 caacgatcgc aagcggaatt cccccgtctc tgacgaatat cccgcgagca cggtcgtaat
      901 acctgagggc catacggttt ttatcgaggc ggtgatagat gttgccgata ttggtcatca
      961 cgcgtgcggc caggttcaga ttgtctttgc gcttgaaata ggccagcgct ttctgacctg
     1021 ccctgagagc atcatcatat ttacccagat acatctccac gtccataagc ccctggcggg
     1081 tgcgtgcagc cagttcaaac tgcctcaacg ccagcatctt tttaatagcg cgccggtatt
     1141 ttcttaaggc cgactgagga ttgccgcacc agttctcata gcgggcctca atcgtccaga
     1201 gcctggcgcg gtaatggtcg ggcagataca aaaataattc ggccgctttc tttactatcc
     1261 tgccagcctc tttgaggttg acatgcaaaa tccggctgac agccttattc agttcagaga
     1321 caaaaatatc cctgtcacct tcataaaact cttttatgaa tgcgtcgagc tcttcctggt
     1381 tcgaaattcc ggatattttc gaatattcta tcataactcg atatcgatct ttgcgataac
     1441 ctctttctcc gcataaattc taatattgta cctgccgctc tcgatacgct ccagcctgaa
     1501 gagctggaaa gcattgctct taaccttttt agtcgattta cggcctttga tttctatttc
     1561 gattatatca cccggctgca gtccggaaag ctgaccgatc aactggtatg atccaggtga
     1621 tatgggatac agtgaaattt ccagtatatc atcgcctaca cgatacttga cccggcgagt
     1681 gctgataatg tccgggcgga catcctcggg caggggcata tctttggaat caaagaagat
     1741 aattccctgc ttagcgtctg tgctcttccg agacagtttg taatccttga gcattcgctc
     1801 cagtacagaa tgggccggtt gtttaagtct gtcccatttg atatcatcgg attcccttat
     1861 gagttccgaa acgaattccg cagttctgct cagttcggga tcttctttga gcattcgctc
     1921 gaactgtctt ttacgatctc cactcaatcg ccccttcaaa taatcggcca gaagatcata
     1981 tttgtcattt tttccggaca ttttaccacc tatatattaa gtcatgatat ttcatacaaa
     2041 aaatacttag aacttgtaca gttcacgttt aagaatcctt cccagctttt ccaatgcacg
     2101 cgccctggtg ggcccgatcg atgaggctgg aacctgcagc tttcgggcaa tctctttata
     2161 tgagggatta ccgggatcaa agaaaagagc tttcagcaat ctgtactgcc gggtagggag
     2221 tttggtcatc gctttcatca ataattcccg tctctgcata ttttcatagg tcagatcggg
     2281 tggctcttcc tgcgattccg ataacatcgg aacaacttcc aatttgaagt agtctgccag
     2341 cctcatcttc tcgtagaaat taaagatttt tcggcgtgtg atggtggcca caaaggacag
     2401 gattctggcc ggcgatttga tcgtaccaat attgcctacc agatcaaaac agacctgccc
     2461 gtaaatgtcg aagctttcat catgggtaag tctgcttttc ctgcaaattg aaaatatcag
     2521 gggaccgata atatcgataa gctcatgcca ggcatcctca tccccttcac tgcagagc
//
LOCUS       DSEB01000132            4094 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002074, whole genome shotgun sequence.
ACCESSION   DSEB01000132 DSEB01000000
VERSION     DSEB01000132.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002074"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="ENO07_02495"
     CDS             complement(<1..1496)
                     /locus_tag="ENO07_02495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HDS00866.1"
                     /translation="MQKYRTTAIFIVLNIAAIIAGIYLVITDPLFEGMMAYAAAFRII
                     NENYADPINPEDLFQGSIENIESGLDPFTGYVPPRSYQYYEEESQGEFSGIGVEITVR
                     DGLLTVVSPIPGSPADRAGLQAGDQVTMIGDIDASTLDPDEAVDLIRGQPGTSVELRI
                     RRPGLKEQFNVKIERASVQISSVEYAGMVNSTGYIRLTNFALNSLNEMVTALESLKMM
                     GMDGLVLDLRGNPGGFLEIAVAITGLFLPDEALIVSTGGRSFTEEFQLRNFFEGDYQD
                     IPIVVLVNNGSASASEILSGALQDHDRAVIMGDTTFGKGLVQNISPLPQDGALRLTVS
                     KYYLPSGRIIQKFSDMAWSENLVKTEPRAETVYRTTGGRKVYGGGGVVPDIIYDDEEP
                     TLLESVLLVGGYYFDFGIEYLRKHGNQLSLPLEPEVLDSFKVYLDSRGLHVSGPIEQA
                     FDDFMQEAKKINPGFSDKSAVSSIRSKISMIENESWEENKNTIMENLS"
     gene            complement(1496..2146)
                     /locus_tag="ENO07_02500"
     CDS             complement(1496..2146)
                     /locus_tag="ENO07_02500"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDS00867.1"
                     /translation="MGKGIFITLEGIDFSGKSTQARYLVNFLKRNNVQHLFLREPGGT
                     VLSERIRRILLSKGEIDICAKSELWLYLAARSQIVEEKIAPALKQGKIVVCDRFYDST
                     TAYQSYGRGLDLDFVLKANLFAADNLKPDLTLLFDLPEKKAFERKQRLNRTKDRLENE
                     KLAFFRKIRKGYLHLAENNKRRIKVINADGPKEKVRDEMLEITVSFLKRHRIRIRM"
     gene            complement(2207..3373)
                     /locus_tag="ENO07_02505"
     CDS             complement(2207..3373)
                     /locus_tag="ENO07_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HDS00868.1"
                     /translation="MPKVRVAIVGVGNCASSFVQGVHYYRNAKDTDQIPGLMHVNLGG
                     YHISDIEFSAAIDIDKNKVGKDLAEAIYTKPNNTYKFADVPKTGIKVKRGMTHDGLGK
                     YLSEIIEKAPGDTVDIIKLLKETRTDVVVNYLPVGSEMATKWYVEQVLEAGCGFVNCI
                     PVFIAREPYWQDRFEKRGLPVIGDDIKSQVGATITHRILTRLFMDRGVKLDRTSQLNV
                     GGNTDFLNMLERSRLESKKISKTSAVTSQLPYDMGADNCHIGPSDYVAWLTDRKWAYI
                     RLEGTTFGDVPLNIEMKLEVWDSPNSAGVVIDAVRCCKLALDNGLSGALIEPSSYFKK
                     SPPVQFTDSEAHRMTEEFIKKYAIKKGSKTAPETSTARKTKKGASKKSAGRKKK"
     gene            complement(3391..>4094)
                     /locus_tag="ENO07_02510"
     CDS             complement(3391..>4094)
                     /locus_tag="ENO07_02510"
                     /inference="COORDINATES: protein motif:HMM:PF00294.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDS00869.1"
                     /translation="YIIYSVEESGGFSLEYYGDKGERNLNLLGEAGRIKMFPEKLAEA
                     DWIVIAPILGEVDLEYIQHIKEHTSARIFLDPQGILRYSEDGRIEHRKMEDTEKIISM
                     CEIVKPNELECKILTGYDPREDYETPAKMLRSWGPETVIITIAEKGSVVYDGEEFFHI
                     PAFETREIDATGAGDTYAAGFMYGLIRGYALPDCGILGTAVASVMIEHTGPDFPLTKA
                     VALERMQKIANSIVA"
BASE COUNT          958 a         1122 c          930 g         1084 t
ORIGIN      
        1 atgctaaggt tttccataat tgtgttcttg ttttcttccc aggactcgtt ttcaatcata
       61 cttatcttcg accttatgga tgataccgcg gatttatcag aaaatcccgg atttatcttc
      121 ttcgcctcct gcatgaaatc atcaaaagcc tgctcgatcg ggccggagac atgcagcccc
      181 ctgctgtcga ggtatacctt gaagctgtcc agaacttccg gttccagggg cagacttaac
      241 tgatttccat gtttccgcag gtactcaatc ccgaaatcaa aatagtatcc tcccaccagc
      301 agcaccgatt cgagaagtgt cggctcttcg tcatcataaa taatgtccgg caccacaccg
      361 ccgcccccat agacctttct tccgcctgtg gtacggtaaa ctgtctccgc ccggggctcg
      421 gtctttacga gattttcgga ccatgccata tcggagaact tctgaattat tcgccccgag
      481 ggcaggtaat acttcgaaac cgtcagcctg agcgcgccat cctgcgggag aggggagata
      541 ttctgcacca gccctttgcc gaacgtggta tcgcccatga taaccgcccg gtcatgatcc
      601 tgcagggccc cggagaggat ttctgaggcg gaagcggatc cattattaac cagcactact
      661 atgggaatat cctggtagtc gccctcaaag aaattcctca attggaattc ctcggtaaaa
      721 gagcgtcccc cggtcgacac gatcagggcc tcatccggca ggaacaatcc tgtgattgcc
      781 acggcaattt ccagaaatcc tcccggattt cccctgaggt caagaaccag cccgtccata
      841 cccatcatct tcagcgattc cagagcggtc accatttcat tcaaactgtt cagggcaaaa
      901 ttagtgagac gtatataccc ggtcgaattg accatgccgg catattcaac tgaactaatc
      961 tgcaccgagg cgcgttcgat tttgacatta aattgctcct tcaagcccgg ccttctgatg
     1021 cggagttcaa cgctcgtgcc cggctggccg cggattagat ccacagcctc atcgggatcg
     1081 agcgtggagg cgtcgatatc tccgatcatg gtgacctggt caccggcctg cagcccggcc
     1141 cggtccgcag gagagcccgg aatgggcgag accaccgtaa gcaggccgtc acgaaccgtt
     1201 atttcaactc ctatgccgga gaattcaccc tggctttcct cttcataata ttgatagctt
     1261 ctgggcggaa catatccggt gaatgggtcg aggccacttt caatattctc aatcgatccc
     1321 tgaaagagat cctcgggatt tatcggatcc gcataatttt cattaataat gcggaatgcc
     1381 gcggcatagg ccatcatccc ttcaaaaaga ggatcggtta taaccagata tataccggca
     1441 atgattgctg cgatatttaa tactatgaaa attgcggttg tccgatattt ctgcactaca
     1501 tcctgatcct gattctgtgc cgcttcagga aagatactgt aatttccagc atctcatccc
     1561 ggaccttttc ctttggccca tcggcattta taaccttaat ccgtctttta ttgttctcgg
     1621 ccagatgcag atagcccttg cgtatcttgc ggaagaaagc cagcttttca ttctccagcc
     1681 gatcctttgt acggttcagc ctttgcttcc tttcaaaggc ttttttctcc ggcaggtcaa
     1741 aaagaagagt aaggtcgggc ttcaggttat cagcagcaaa caggttggct ttcagaacaa
     1801 aatcaagatc gaggccgcgt ccataagact gataggcggt ggtggaatca tagaagcggt
     1861 cgcagaccac aattttgcct tgcttgaggg cgggggcaat tttctcctcc acaatctgtg
     1921 aacgcgccgc cagatacagc catagctcgg atttggcgca gatgtctatc tcccctttgc
     1981 tcaacaaaat ccggcgtatt ctttcagaaa gcacggttcc gcccggctct ctcaggaaga
     2041 gatgctgaac attatttctc ttcagaaaat ttacaagata tctggcctgc gttgattttc
     2101 ccgaaaaatc tatgccttcg agagtaataa aaatgccttt tcccataacc ataatatata
     2161 aaaaaagaag gcggcggagg ccaccttctt ttataaagat gataatttat ttcttcttcc
     2221 tgccggcgct ctttttggaa gcgccttttt tcgttttcct ggccgtagag gtctccgggg
     2281 ccgttttgga gcctttcttg atcgcatact tcttgatgaa ttcctcggtc atgcgatggg
     2341 cctcggaatc ggtaaactgc accggcggag atttcttgaa atatgatgat ggttcgatca
     2401 gcgctcccga aagcccatta tcgagggcca gtttacagca gcgcacagca tcgatcacca
     2461 caccggcgga gttgggcgaa tcccagacct ccagcttcat ttcgatattg agaggaacat
     2521 cgccaaaagt cgtaccctcc agacggatat aggcccattt gcggtcggtt aaccaggcca
     2581 cataatcgga tggtccgata tggcaattat ccgcgcccat atcataagga agctgtgagg
     2641 tcacagccga ggtcttggaa atctttttgg attccagccg cgaacgttcg agcatattca
     2701 gaaaatcagt gtttcctcca acattcaact ggctggtgcg atccagcttg actccgcgat
     2761 ccatgaatag acgggtcagt atccgatggg ttatggttgc tcccacctgg gacttgatat
     2821 catccccgat aaccggaaga cctctttttt cgaagcgatc ctgccagtag ggctccctgg
     2881 ctatgaatac cggtatgcag ttcacaaatc cgcatccggc ttcgagcacc tgctcgacat
     2941 accatttggt agccatctcc gagcccaccg gaagataatt gaccacaaca tcggtcctgg
     3001 tttcttttaa tagcttgatg atgtccactg tatcgccggg agctttttca atgatctcgg
     3061 acagatattt ccccaggccg tcatgcgtca taccgcgttt aaccttgatg cctgtcttag
     3121 gaacatctgc gaacttatag gtattattcg gtttggtata gatagcctcg gcaaggtctt
     3181 tgccgacctt gttcttgtcg atatcgatgg cggcggaaaa ttcgatatcg gaaatatgat
     3241 agccgccaag attgacatgc atcagcccgg gaatctgatc ggtatccttc gcattcctgt
     3301 aataatgcac tccctggaca aaagatgatg cgcaattgcc gacaccgaca attgccactc
     3361 tgacttttgg catattatct cctttttaat ttatgctaca atagaatttg ctattttctg
     3421 catcctctcc agcgcgaccg ccttggtcag cgggaagtcc gggccggtat gttctatcat
     3481 cacgcttgcc accgcagtac ccaggattcc gcaatcgggc agggcgtaac cgcgaatcag
     3541 gccgtacata aagccggccg cataggtatc tcccgctccc gttgcgtcaa tctcccgcgt
     3601 ctcaaatgcc ggtatgtgga agaactcctc accgtcataa actacagacc ccttctcggc
     3661 gattgttatt attaccgttt ccgggcccca tgatctcagc attttggccg gtgtctcata
     3721 gtcctcgcgc ggatcatacc cggtcaggat cttgcactcc agttcattgg gtttaacaat
     3781 ctcgcacatg gaaataattt tctcggtatc ctccatcttg cgatgctcga tcctgccgtc
     3841 ctccgaatac ctcaggatac cctgcgggtc aagaaatatg cgggccgagg tgtgttcctt
     3901 gatatgctgg atatattcga ggtcaacctc gcccagtata ggcgcaatca cgatccagtc
     3961 ggcctcggcg agcttctcgg gaaacatctt gatcctgccg gcctcaccca gcagattcag
     4021 gttcctctcc cctttgtctc cataatattc aagagaaaat cccccgctct cctcaacgct
     4081 gtaaattata taat
//
LOCUS       DSEB01000133            3424 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003070, whole genome shotgun sequence.
ACCESSION   DSEB01000133 DSEB01000000
VERSION     DSEB01000133.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003070"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..255)
                     /locus_tag="ENO07_02515"
     CDS             complement(<1..255)
                     /locus_tag="ENO07_02515"
                     /inference="COORDINATES: protein motif:HMM:PF01839.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00870.1"
                     /translation="MDTIADIIMPYNSVSPSSYNFGRSVGPAGDFNGDGVDDLAVGAK
                     SYNTQNSSGYLYVFAGDPDLPTPAEDEPDIPIPEDHNILKQ"
     gene            complement(234..1328)
                     /locus_tag="ENO07_02520"
     CDS             complement(234..1328)
                     /locus_tag="ENO07_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00871.1"
                     /translation="MNSIFSAVKALFRALIFAPCIFLFVSSYGYSIDLELIFLAEGEN
                     HSENLGFFVQSPGDLDQDGYSEVFAMNISSEIKVFRGGNPADTLPDKIYYSYGGRIAW
                     LPDINDDGTGDFAIGKEDAENEYIEFYFGGPGFYEKSKADLTIVGNKSEGFGITIFSS
                     VTNNDNSRDLVIAAPNTSSPIAGRFYIYQGCSDLDSIGDDTITIARTERDDFYTGTCV
                     GDINGDQYADYAITPYSGAVPSYILIFWGSENPDSIPDMQIASPFEDAPGIGAFGYDI
                     IATGDINKDNIDDFIVTSEGFPPCIFYGGNPFNPTPKILEYPGKVANVCGDINHDGWE
                     DIAVGFVSYSFGSGEVLVYLAHMIWIPSLI"
     gene            complement(1438..3068)
                     /gene="pruA"
                     /locus_tag="ENO07_02525"
                     /pseudo
     CDS             complement(1438..3068)
                     /gene="pruA"
                     /locus_tag="ENO07_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419889.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
BASE COUNT          943 a          865 c          716 g          900 t
ORIGIN      
        1 ctgttttaga atattgtggt cttcaggaat tggtatatcc ggctcatcct ctgcaggtgt
       61 gggtaagtca ggatcaccag caaaaacata taaatatcca ctactgtttt gcgtattata
      121 tgactttgcc ccgactgcca gatcgtctac tccatcacca ttgaaatccc cggccggacc
      181 aacggagcga ccaaaattat aagatgaggg agacacagaa ttatatggca taattatatc
      241 agcgatggta tccatatcat atgcgccaaa tatacaagaa cctcccccga tccgaatgag
      301 taagacacaa agccaaccgc aatatcctcc cagccatcgt gatttatgtc accacagaca
      361 tttgccacct ttccaggata ttccagtatt ttaggcgttg gattaaatgg attacctcca
      421 taaaatatac aaggcggaaa accttcagag gttacaatga aatcatcaat attatctttg
      481 ttaatgtcgc ctgtggcaat aatatcatat ccaaatgctc ctatcccggg agcatcttca
      541 aacggggaag ctatttgcat atcaggaatt gaatctggat tttcacttcc ccaaaatata
      601 agaatataac taggaacagc cccggaataa ggcgtgatag cataatctgc atactgatcc
      661 ccatttatat ctccaacgca agtgcctgtg taaaaatcat ccctttcggt ccgagctatg
      721 gttattgtat catcccctat cgaatccaaa tcggaacatc cctgataaat gtaaaacctc
      781 cccgcaatcg gcgaactagt gtttggggcc gcaataacca gatcccttga attatcattg
      841 ttagttacag aggaaaaaat cgtaattccg aaaccctccg acttattacc aacaatggtc
      901 aaatctgcct tagacttttc gtagaatcca ggacctccaa aataaaactc tatatattca
      961 ttctcagcat cctctttccc tatcgcaaag tctccagttc catcgtcatt tatatcaggt
     1021 agccaagcaa ttcttcctcc ataactataa taaattttat ccggaagagt atctgcagga
     1081 tttccgcccc taaagacttt gatttcactg gatatattca ttgcaaacac ctctgaatac
     1141 ccatcctgat ccaggtcgcc tggcgattga acaaaaaatc ctaaattttc ggaatgattt
     1201 tctccctctg caagaaaaat cagctccaga tcaattgaat agccataaga agatacgaac
     1261 aagaagatac aaggagcgaa gattaatgct ctaaataaag ccttaacggc agaaaatata
     1321 gaattcaatg ttcagcatcc ggctacgtgg ttcatttaca aaataattaa ctggaatccg
     1381 acgtcaatta aaaaagcccc atctttcgac agggcttttg catcaaagca agattgttta
     1441 ctctttttcc ataaacggat acttataatc agtcggcggt acgaagttct ccttaattgc
     1501 gcggggtgaa caccagcgaa tcaggttcag aagcgaaccg gccttgtcgt tggtacccga
     1561 ggcacgtgcg ccgccaaaag gctgctgtcc cactaccgca ccggtgggct tgtcgttgat
     1621 atagaaattg ccggcggcat gtgtcaggac tttctcagct cttacaatcg cctcacgatc
     1681 ccgtgcaaag acagccccgg tcagggcgta tggtgaggtc tcatcacaga gcttcaaggt
     1741 ttcttcgtaa gcattatcat catagatata aacagtcagg accgggccga atatctcctc
     1801 ctccatcagc ttgaaatggg gattgccggt ttcgatcatg gtcggctcga taaaatagcc
     1861 cctggaatcg tcataattgc cgccgaagat gaaattggct tcatcggatt ctttggcgta
     1921 gtcgatgtat tccacaatgc tgtcgaaggc gcccttgtcg atgactgcgt tgataaacat
     1981 gctgaaatct tcaacatcac ccattttcgc ctctttgctg aacccgacca taatctcttt
     2041 gatcttgggc cagagtgatt ttggaatata ggcacgggaa actgcagaac atttctgtcc
     2101 ctggtactca taggcgcccc ggaatagagc ggtcgcggtg gcatacggct cagccgagga
     2161 atgcacgaag acgaagtcct tgccgccggt ttcccctaca atcctggggt aggatttata
     2221 gttggcgata ttctccccga caactttcca catattctga aaaaccgcag tggatcccgt
     2281 aaaatgaaca ccggcaagat ccctgctttt gagcaccagt tcgccgatcg ccgcacccgg
     2341 agcggtaacc atgttgataa ctccgtccgg caacccggct tccttcatta cctgcatgac
     2401 gatatgggct gtataaaccc ggtcgaagcc ggcttccaga ccacggtatt gcccatcatg
     2461 gcgggcgagg tcggaaggtt tccgccgatg gagacaaaat tgaacggcgt gaccgcaaag
     2521 acgaagccct ccaggggacg atattccatc ctgtcccaga tcatgggggc gttgcccggc
     2581 tgcatatcat aaatcatctg ggcgtagaag ggattataac ggtagaagtc aaccagctca
     2641 cagaccccgt cgatctcggc ctggaagaca tttttggaat gcgccagcat ggtggcggcg
     2701 ttcatgatat agcggtacgg cccggccaac agatcggccg ccttaagaaa tatccccagc
     2761 cggtgctggt agggcatatc ggcccacttc tgcttggcat ccatggccgc cttgatggcc
     2821 aacttgactt cctctgcgga gcctttggaa tattcgccca gtttcaggga gagcttatga
     2881 ggcgccttga tttcggcctt tttttcggtt cgaacttcct tgcctccgat caccatgggt
     2941 atgtcggccg gattcgattt caaatccgcg atcgcctttc tcaatttcgc gcgttcggga
     3001 gaaccgggtg cataggaata caccggctca ttcttcggtt gcggaattct gaaactactc
     3061 atttgtatcc tccaatttga tttttatttc aaatccagca cgttgatctt caaagcgcta
     3121 aaattcatca aaaacatcaa actgtcaagt gtttcataaa taaatcgaag cggccgaggg
     3181 cgggggtttt gcttaattga tttgacatgc caactatacg cacacaccta ttaacgcttc
     3241 aatatctcga aaaacaatgt atcaaatcat caactagttc atctatatta aggacggtcc
     3301 aaatgcttga aaatatcctc acctacatag tgattgctgc agttgttggc attctcggca
     3361 tcccgttata taaactcctg cttgccattt ttgatcccaa aggcaagcac taaacccttt
     3421 aaac
//
LOCUS       DSEB01000134            2428 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1006592, whole genome shotgun sequence.
ACCESSION   DSEB01000134 DSEB01000000
VERSION     DSEB01000134.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1006592"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(100..1359)
                     /locus_tag="ENO07_02530"
     CDS             complement(100..1359)
                     /locus_tag="ENO07_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDS00872.1"
                     /translation="MGHLAGKDLYRKLGKKIDGLTARAPWNDSLYAILKELYSEDEAE
                     VAVKMPYGVHPLEYIQKTTGLNRDKLVKTLESMADKGLVIDMCHGERCFYTLSPMVVG
                     IFEFTMMRTGEGSDHKIWARLMNDYMHGDDSFYSSNLKDKNISLPLRTLPHEEAVDPG
                     EFSEILDYEKATEIVKSARRFAIGICSCRHEKYHLGEKKCDVPLEMCSSFDSAADYLI
                     RHNMGKEVSKEEMLENVAKARDMRLVFNADNVKKEVGFICMCCGCCCNALAGIKEYGY
                     PNAVVTSRFIAKHDEDDCIGCGECAEACPIDAIAMDDDDNPVVDESICIGCGVCALEC
                     ATESMKLRERGQRVLYPEDTFERVILQSLNNDTLQNLIFNNPQSKSHGFMRAVVGGFL
                     KLPPVKQALMGDKLRSRFLSKMREKSR"
     gene            1816..>2428
                     /locus_tag="ENO07_02535"
     CDS             1816..>2428
                     /locus_tag="ENO07_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00873.1"
                     /translation="MRNAIFIAVVFYLLLMPAALQAQNEDTVKIVSIQVDPDPVNPVK
                     FGLPVNLVVTGNPGNMDDQISAASLGHYFDPGDYVTIDSVRLTGGLAWGNIAWNGSYH
                     DDAQNLGVIGFVLMYPNYILPGESGLLGTLWFTLDANAPDHVIEIDSGYFPPAGDYIL
                     TNGNGVSIYPEFVAGTITVGTGISSELVVSPTALTFEATVGGTD"
BASE COUNT          602 a          578 c          558 g          690 t
ORIGIN      
        1 atttataagc agttcgggta ttcggttcgt tgtgtcgggg attgataaga tattcttatt
       61 ggcgattaaa atccggcagc gcactgcgct ttttcagatc taccgggact tttctctcat
      121 cttacttaaa aaccgcgagc gcagtttgtc acccatcaga gcctgcttga ccgggggcag
      181 tttcaagaat ccaccgacaa ccgcacgcat gaaaccgtgc gatttactct gaggattgtt
      241 gaatatcagg ttctgcaggg tatcattgtt gagggattgc aggattaccc tttcaaatgt
      301 gtcctcgggg taaagcactc tctggccacg ctccctgagc ttcatcgatt cagttgcgca
      361 ctcaagcgca catacgccac agccgataca tatcgattca tccaccacag gattgtcatc
      421 gtcgtccatt gcgatcgcat cgatcgggca ggcctcggcg cattcaccgc atccgataca
      481 atcatcttcg tcgtgtttgg caatgaacct cgatgtgacc accgcattcg ggtaaccgta
      541 ctctttgata ccggccagcg cattacagca gcatccacag cacatgcaga taaagccgac
      601 ctcttttttg acattgtcag cattgaagac cagacgcata tctctggctt tggcgacatt
      661 ttcgagcatc tcttccttcg acacctcttt acccatgtta tgccgaatca aatagtcggc
      721 ggcagaatca aaagaagaac acatctccag gggcacatcg catttttttt cacccaggtg
      781 atatttctcg tgacggcagg agcagattcc gattgcaaat cgtctggctg atttaacgat
      841 ctctgtcgct ttttcgtaat cgagaatttc cgaaaattcg cccgggtcta ctgcttcttc
      901 atgcgggaga gttcgcagcg gtaggctgat atttttatcc ttaaggttgg agctgtaaaa
      961 ggaatcatca ccatgcatat aatcattcat gagccgcgcc cagattttat ggtcgctgcc
     1021 ttctccggtg cgcatcatgg tgaattcgaa aattcctacc accatcggcg aaagcgtgta
     1081 aaaacaacgc tcaccatgac acatatcgat cacgaggcct ttatccgcca tactctcaag
     1141 tgtcttcacg agtttgtctc tattgagtcc ggtagtcttc tggatatact ccagcggatg
     1201 cacgccgtag ggcatcttga ccgccacctc ggcctcatcc tcggaataaa gttcctttag
     1261 aatggcgtat agcgaatcat tccatggcgc gcgtgcagtc agcccgtcga tcttttttcc
     1321 cagtttgcga taaagatcct tgccggccag atggcccata atgtcctccg gatatggatt
     1381 cctgaatata aagcaaaaaa tattactcaa ggtaaaattt ggcaatgtaa atctttcatc
     1441 tatatggctt cattttaagt ccaatggaag cagtaagagc caaccacatt agggataaac
     1501 atattccggg agtctttttc ttcttgacat tttccttcaa aatagtatat tttttatact
     1561 tcaagtaaag caacgggggc aagtaccttt attaatcgga aagtcggtaa cgtcttttcc
     1621 aacaattcta tattttcggg ggcattaata gatacaggtt caatggggta tggctgcttg
     1681 gatagacgaa ttttatctta tccgagcggg aggaaaatct tatgtgcaat ggaccttgat
     1741 tagatgacct gactgcgtat tgcagttgat cgaatatgca gtatgaattt tgatttatta
     1801 aaagtaggag gtacaatgcg taatgctata tttattgccg tggtcttcta tcttctatta
     1861 atgcctgcgg cgctgcaggc gcagaacgaa gacacggtaa agatcgtcag tattcaggtt
     1921 gaccccgatc ctgtgaatcc ggttaaattc ggcctgcctg tcaaccttgt agtcacaggg
     1981 aatccaggaa atatggatga ccaaatttct gcggcatctc tgggacacta ctttgatccc
     2041 ggcgattatg tcacaattga ctcggtcagg ctgacaggag gcctggcttg gggtaatatc
     2101 gcttggaacg gatcttatca cgatgatgcg caaaatctcg gagttatcgg atttgttctt
     2161 atgtatccca attatatcct tccaggcgaa tccggactgc tggggacgtt atggttcacc
     2221 ctggatgcca atgctccgga tcatgtgatt gaaattgact ccggctattt cccgcctgca
     2281 ggagattata tacttaccaa tggtaatggt gtatcaatat atccggaatt tgtggccggg
     2341 acaattactg tcgggacggg aatatcgtcg gagcttgtgg tgagccctac ggccctgact
     2401 ttcgaggcca ctgtcggagg cacagacc
//
LOCUS       DSEB01000135            1580 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1017067, whole genome shotgun sequence.
ACCESSION   DSEB01000135 DSEB01000000
VERSION     DSEB01000135.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1017067"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            563..>1580
                     /locus_tag="ENO07_02540"
     CDS             563..>1580
                     /locus_tag="ENO07_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HDS00874.1"
                     /translation="MAEHVDKKSNVIHGPSLLSEYDVYLFRQGNHYRIYEKLGAHRIV
                     VDGREGTLFSVFAPNAEKVFVMGDFNDWNPSSHPLAVRWDGSGVWEGFVPDARKGQLY
                     KYHIKSKYNNYEVDKGDPFAISWELSPRTASQVWDLEYKWHDEGWMNTRGERNRMDGP
                     ISIYEVHLGSWRRKPEENNRYLGYREIAHDLAEYVKEMGFTHVELLPIMEHPFYGSWG
                     YQTAGYFSPTSRFGSPQDFMYFVDYLHSHDIGVILDWVPSHFPSDNHGLVYFDGTHLF
                     EHEDPRKGFHPDWQSYIYNYGRNEVRNFLLASAMFWLDQYHIDGIRVDAVASMLYLDY
                     SRKEG"
BASE COUNT          417 a          349 c          417 g          397 t
ORIGIN      
        1 agatgatccg caaatcggaa gacgagatat tgaaacgaat gaagagaatc ctgcgcaaga
       61 aaatgtcagc aaaaagaacc cgaatacatg gagattacca tctggggcag gtgcttaaca
      121 cgggcaaaga ctttgtaatc ctcgatttcg aaggcgagcc cgccagaccc attagcgaac
      181 gcaggctgaa gcattcgcct ttaaaagatg tcgccggtat gattcgctcg attcattatg
      241 tagcctattc atatttcatg acccgtccga aattgcaggc tgaacagatg aatctgcttg
      301 agtcctggat tgagccatgg tatctttatg tttccggggt attcttcgga gcatatatgc
      361 aaacggtaaa cgaatcaaaa attattcccc gtgagaatgg cgaactggaa acgctgttgc
      421 agacttttct actggagaaa gggatctatg agctcgggta tgagcttaat cacaggcccg
      481 actggataac tattccgatt cgcggcattg aatatctatt ggttagttaa tttcttaata
      541 tatcagcaag aggagaataa atatggctga acatgtagat aaaaaatcaa atgtgataca
      601 tggtcccagc cttttgagtg aatatgatgt ttatcttttc aggcagggaa accactacag
      661 gatatatgaa aaactggggg cgcacagaat tgtagtggat ggccgagagg gcacgttatt
      721 ttcggtcttc gctcccaatg ccgaaaaagt gtttgtgatg ggcgatttca atgactggaa
      781 tccttcctcg catcccctgg cggtcaggtg ggacggttcc ggtgtgtggg aaggatttgt
      841 gccggatgcc cgcaagggcc aattgtataa atatcacata aaatcaaaat ataataatta
      901 cgaggtcgat aagggcgatc cttttgcgat ctcctgggag ctttcaccca ggacagcctc
      961 ccaggtctgg gatcttgaat acaaatggca tgatgaaggc tggatgaata ccaggggtga
     1021 aagaaacaga atggacggcc cgatttcgat ttacgaggtg catctgggct catggcggcg
     1081 caagcccgag gaaaataatc gttacctggg ttaccgcgag atcgcccatg atctggccga
     1141 gtatgtaaaa gagatgggct tcacgcatgt ggagctgttg ccgattatgg aacacccttt
     1201 ttacggctcc tggggttatc agacagcggg atacttttcg cccaccagcc gcttcggcag
     1261 cccgcaggac ttcatgtatt ttgtggatta tctgcacagc catgacatcg gcgtgatcct
     1321 ggactgggtg ccctcgcact tcccctccga caaccatggg ctggtctatt ttgacggtac
     1381 ccatctgttc gaacacgagg atccgcgcaa ggggtttcat cccgactggc agagttatat
     1441 ctacaactac ggccgcaacg aggtcaggaa tttcctgctt gccagtgcca tgttctggct
     1501 ggatcagtat catatcgacg gcatcagggt ggatgccgtg gcgtccatgc tgtatctgga
     1561 ttattcccgc aaagagggtg
//
LOCUS       DSEB01000136            5466 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001077, whole genome shotgun sequence.
ACCESSION   DSEB01000136 DSEB01000000
VERSION     DSEB01000136.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001077"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            168..1016
                     /locus_tag="ENO07_02545"
     CDS             168..1016
                     /locus_tag="ENO07_02545"
                     /inference="COORDINATES: protein motif:HMM:PF01063.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine aminotransferase Dat"
                     /protein_id="HDS00875.1"
                     /translation="MIVFLNGEFLDKQHVQISPDDRGFLFGDGLYEVVLAYNGRPFRL
                     RDHIARLKCGCEFARIKLENIEYVREVCALLIEKNDLEDVIANIYIQVTRGAYKRAHH
                     FPPGDVLPTLYISAGEFSPNRAHQKNGIKAVTVPDDRWGHCNIKTIDLLPNVLAHQRA
                     FDQGGTEAIFVRDGVLLEGTHSNLFAVKDGKVYTHPRSNRVLSGVTRRTVIEICSRMD
                     IDLLMEAIEESMVEKLDEAFVTSTTLEITPVVAVNDLQIGNGKPGKITTLIQDEYQTL
                     VRQGHD"
     gene            1069..2268
                     /locus_tag="ENO07_02550"
     CDS             1069..2268
                     /locus_tag="ENO07_02550"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDS00876.1"
                     /translation="MPTTNTTAKDIFCPRCAKHYDITAVLTLCECGAPLLVDYDYEKA
                     SSVIDREKLKGRTANMWRYLEVLPVFDCDNVATLGEGGTQLLVSKTIGPELGMPDLFL
                     KDETTNPTGSFKARGLAMAVSRARELGLKKLIIPTAGNAGSALAAYAARAGLECKIIM
                     PRDVPGPFLVDAKYHGAQIELVDGTIKDCGERATDLVKNEGWFSAATLKEPYRIEGKK
                     TMGYELAEDFNFELPDVIIYPTGGGTGLIGMWKAFDEMERMGWIGSFRPKMVSVQAEG
                     CAPIPRAYEEGLDYAPVWEDPHTLAAGLRVPGAVGDFLMLEAVRKSGGTAVAVSDNDL
                     MMDTKELSAKEGIFSSPEGGATLTCLKMLLNDGFVKPDERVVLFITGSGYKYLDVMQE
                     WDEEHSA"
     gene            2271..2834
                     /locus_tag="ENO07_02555"
     CDS             2271..2834
                     /locus_tag="ENO07_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HDS00877.1"
                     /translation="MNLIVIIGIAVGLAMDAFAVAISASAIIGKITGRHVFRLGFHFG
                     LFQAMMPVIGWLAGSRIQKYIAPVDHWIAFGLLAFIGIKAIYGALTREIEPGTQCDPS
                     RGVSLVMLSVATSIDALAVGLSFAMLNINILYPALIIGVITGAITIIGMVIGSSFGAK
                     LGKPMEIIGGLVLIGIGVKILIEHLMM"
     gene            2954..3673
                     /locus_tag="ENO07_02560"
     CDS             2954..3673
                     /locus_tag="ENO07_02560"
                     /inference="COORDINATES: protein motif:HMM:PF07610.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HDS00878.1"
                     /translation="MHKVSTAILTVCIFLIPSAISAQPKMEFQHSYFDFGYSPQNAEL
                     THDFWLKSVGDENLVINNIKTGCSCTKAPLEKDVIAPGDSARLKIIFDTGRYKGRTFK
                     RPIVETNEGSDLRQLTIIANIVTAQDSMFPLVVEPLSLEFMIDAGEPKHTKKIEITNI
                     SDQDYELKLIEYPANLVRIELPEKIPAGETAVAVVKLTPDAVKTKFSSSFTLEINDED
                     ETRYSVGVTGRPGTPKLKMNY"
     gene            3699..4484
                     /locus_tag="ENO07_02565"
     CDS             3699..4484
                     /locus_tag="ENO07_02565"
                     /inference="COORDINATES: protein motif:HMM:PF02649.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00879.1"
                     /translation="MPKEKGILVDVGMKGMPFPFRVLSREESEGQYTIANLSVIARID
                     NRFESRWIDKFIQIIHKNRFKVGTQTLHESIADYLKELKATMVRVDFDYPFFVEKKLP
                     ATGEKCLVRYMCTYNAKLSSVGDNPKIIFKIHVPAITTDPASDPEKEGGLFGQLSVVT
                     IEVDSRKEIFPEDLVDLVDKYSISPVYSYLNPEDQIFIIQKAHTVEVSSTYLTEQLKT
                     ELANNRYVDWFSVKCSNFGMLHSYNTGIGTEKDLWMPFHSIDE"
     gene            4556..5029
                     /locus_tag="ENO07_02570"
     CDS             4556..5029
                     /locus_tag="ENO07_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00880.1"
                     /translation="MNNGLVQEFIDYGKELLSKHPDLPHEWKINRDKPRAELKFPRQS
                     VSGFDVVIEAKSSEITVRTGELGHEHFEPEEGPPVYLVESVFGYVRDLLSPDMRLREK
                     LAGNKPRWAAVESFDETNWNPESVFGLVLWNFFAKKSEKIYTNQQLPGRLSQKNN"
     gene            5168..5359
                     /locus_tag="ENO07_02575"
     CDS             5168..5359
                     /locus_tag="ENO07_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00881.1"
                     /translation="MLTFILWCILFVICWPVAIIALILYPFIWLLLLPFMLLGFAVGG
                     ILAFFKALFYLPARIIGGR"
BASE COUNT         1402 a         1187 c         1467 g         1410 t
ORIGIN      
        1 acccacatca tgtataagcc gagggctgat tggtttgtca atataaagat ttgttgagtt
       61 tgctgacatc gccgaagact ctgcgagaat tatgtttcgg tacgggccag ttttgattat
      121 tattgagtga ccaatattag cgctcggatt atgcatggga cggatttgtg atagtttttc
      181 ttaacggcga atttttggac aagcagcatg ttcagatttc tccggatgac cggggatttc
      241 tatttggtga cgggctttat gaggttgtgc tggcatataa cggcaggcca ttccgtctaa
      301 gggatcacat agcccgtctt aagtgcggct gtgaatttgc ccggatcaag ctggaaaaca
      361 ttgaatatgt tcgtgaagtc tgcgccctgc ttatcgagaa aaacgatctg gaggatgtga
      421 ttgccaatat atatatccag gtaacccgcg gtgcttataa acgcgctcat cacttccctc
      481 cgggtgatgt ccttcccact ctgtatatct cggccggcga attcagtccc aatcgagcgc
      541 atcagaaaaa cggaatcaag gccgtaactg tgccggatga ccgctgggga cattgcaata
      601 tcaagaccat cgaccttctg cccaatgttc tggcgcatca aagagcattc gatcaaggcg
      661 ggacagaagc gatttttgtc agggacggag tcctgctgga gggcacccac agcaatctct
      721 tcgctgttaa ggacggaaag gtttatacac atccccgatc aaacagagtc ctatccggcg
      781 tgacccgcag gacagttatc gagatctgca gcaggatgga cattgatttg ctgatggaag
      841 cgatagagga gagcatggtg gaaaagctgg atgaggcatt tgtcaccagc acgacgcttg
      901 aaattactcc cgtggttgcg gtcaatgatc tgcagatcgg caatggtaaa ccggggaaga
      961 taacgacttt aattcaggac gaataccaaa cactcgtccg tcagggacat gattaagatg
     1021 tccatattct agggagaacg tgaactgaca gcgggagaat gacacattat gcccacaacc
     1081 aacacaacag ccaaggatat attctgcccc agatgcgcca aacattatga tataactgca
     1141 gtgctgactc tatgcgaatg cggcgcgccg cttctggtcg attacgatta cgaaaaggct
     1201 tccagtgtta ttgacaggga aaagctcaaa ggcaggactg ccaatatgtg gcggtacctg
     1261 gaggtgctgc ctgtctttga ttgcgacaac gtcgccacgc tgggcgaggg cggcacacag
     1321 cttctggttt ccaagactat cggccctgag ctcggtatgc ctgacctgtt cttaaaagac
     1381 gagaccacaa acccgaccgg atcattcaaa gcccgtggtt tggccatggc cgtcagccgg
     1441 gccagagagc ttggtttgaa aaagttgatt attcctaccg ccgggaatgc cggttcggcg
     1501 cttgccgcct atgccgcgcg ggccgggctg gagtgcaaga tcatcatgcc gcgggatgtc
     1561 ccggggccgt ttctggtgga cgcgaaatat catggcgccc agattgaatt ggttgacggc
     1621 acgatcaagg actgcgggga gagggcgacg gatctggtca aaaatgaggg ctggttttct
     1681 gcggccactc tcaaggagcc atatcggatt gaaggcaaga agactatggg atatgagctg
     1741 gcggaggatt tcaatttcga gcttcctgat gttataatat atcccaccgg cggcggaacc
     1801 ggcctgatcg gaatgtggaa agcttttgat gagatggaac ggatgggctg gatcggcagc
     1861 ttccgcccga agatggtgtc ggttcaggca gagggatgcg ccccgatacc aagggcttac
     1921 gaagaaggtc tggattatgc tccggtctgg gaagatccgc atacattggc ggccgggctg
     1981 cgtgtgccgg gcgcggtggg ggattttctg atgctcgagg cggtgcgaaa aagcggtggg
     2041 actgcggtgg ctgtttctga taatgatctt atgatggata caaaggagct ttctgccaaa
     2101 gaggggatat tctcatcccc ggaaggcggg gctaccctta catgtcttaa gatgcttttg
     2161 aatgacggct ttgttaagcc ggatgagagg gtggtgcttt tcattacagg aagcggatac
     2221 aagtatctgg atgtgatgca agaatgggac gaagagcata gcgcataata atgaatctga
     2281 ttgttatcat aggtatcgct gtcggtctgg ccatggatgc ttttgcggtg gcaatctcag
     2341 ccagcgcgat aattgggaag ataaccggca ggcatgtttt tcgcctgggc tttcattttg
     2401 gactgtttca ggctatgatg ccggttattg gctggttggc gggcagccga atccagaaat
     2461 atattgcgcc tgtcgaccac tggatagcat tcggactcct ggcatttatc ggaataaagg
     2521 cgatatacgg cgctttgacc agagagatcg agcccgggac ccaatgcgac cccagccggg
     2581 gagtcagttt ggtgatgctt tctgtggcca cgagcattga tgcacttgct gtcggcttga
     2641 gctttgccat gcttaacata aatattttgt atccggccct gattatcggg gtaatcacag
     2701 gagcgattac gatcatcggc atggtgattg gaagcagctt tggtgcaaaa ttgggtaagc
     2761 cgatggagat aatagggggg cttgtattaa ttggtatcgg ggtaaagatt ttgatcgaac
     2821 atctgatgat gtaacctatt ttattctaat aatcagccga tcttgtcttg ccctcgaatt
     2881 aaatttgtat cctggaacgc gaattcctga atttggtatt atattaatag caacgaacaa
     2941 cgcaggaggt tttatgcata aagtttctac agctattctt acagtttgca tatttctgat
     3001 cccatctgct atcagcgctc agcctaagat ggaatttcag cactcctatt tcgattttgg
     3061 atattctccg cagaatgccg agttgacaca tgatttctgg ctgaaatcgg tgggggatga
     3121 gaatctggtt atcaataata tcaaaaccgg ctgcagctgc accaaggctc cgctggaaaa
     3181 agatgttatt gcgccggggg acagcgcgcg gttgaaaatt attttcgaca ccggcagata
     3241 taagggccgt acatttaagc gcccgatagt tgagacaaat gagggttcgg atttgcgcca
     3301 gctcacgatt atagcgaata tagtcacggc tcaggattcc atgttcccgt tagtggttga
     3361 acccctgtct ctggagttca tgatcgatgc aggcgagccc aaacatacca agaaaatcga
     3421 gattaccaat atctcagacc aggattatga gcttaagctt attgaatatc ctgctaatct
     3481 ggtgagaata gaactgcctg agaagatacc tgcgggcgag actgctgtgg ctgtcgtaaa
     3541 gctgaccccg gatgccgtta aaacaaaatt ttcgagttca tttacattag aaattaacga
     3601 cgaggacgag accagatatt cggtcggggt gaccggcaga cccggcactc ccaagctcaa
     3661 gatgaattat tgagtcggga ctgtctcagg aggaagaaat gccgaaagaa aagggaattc
     3721 tggttgatgt gggtatgaaa ggtatgccct ttcccttcag agtgctctct cgtgaggaga
     3781 gcgaagggca atacaccata gcgaatttat ccgtgatagc ccgtatcgac aatagattcg
     3841 aatcgcgctg gattgacaaa tttatccaga tcatccacaa gaacaggttc aaggtcggca
     3901 cccagaccct gcatgagagc attgccgatt atttgaaaga actcaaggcc accatggtca
     3961 gggttgattt cgattatccg ttttttgtgg aaaagaaact gcctgctacc ggggagaaat
     4021 gtctggtcag atacatgtgc acttataatg ctaagttgtc ctctgtcggg gataatccga
     4081 agatcatctt caaaattcat gttcccgcta tcaccaccga tccggcctct gaccctgaaa
     4141 aggaaggcgg tctattcggc cagttgagtg tggttacgat tgaagtggac tccagaaagg
     4201 aaattttccc cgaggatctg gtcgacctgg tggataaata ttcgatatct ccggtgtatt
     4261 cctatttaaa tcccgaagat cagatcttta taatccagaa agctcatact gtcgaagtca
     4321 gcagcactta tctgacagag cagctcaaga ccgaacttgc caacaacagg tatgtggact
     4381 ggttctcggt caaatgctcg aacttcggga tgctgcattc atataatacc ggaatcggaa
     4441 cggagaagga cctgtggatg ccattccaca gcattgatga gtagtgatac attaatatca
     4501 caggatcaag ggtctttctt cctgctgttg taccgctgga aggagtgaga tgattatgaa
     4561 taacggcctg gtgcaggaat tcatagacta cggcaaggaa ctgctttcaa aacatccgga
     4621 cctgccccat gaatggaaga taaaccgaga caagccccga gctgaattga aattcccgcg
     4681 ccagtcggtc tccggctttg atgtagtgat tgaagcgaaa agttcagaaa taactgtacg
     4741 caccggcgaa ctgggacatg aacattttga accggaggag ggaccgcctg tctacctggt
     4801 tgagagcgtg ttcggatatg tgcgtgatct tttgagcccg gacatgaggc tcagggagaa
     4861 gctggccggc aataaacccc gctgggcggc agtggagtcg tttgatgaaa cgaattggaa
     4921 cccggagagc gttttcgggc tggtcctgtg gaacttcttt gccaaaaagt cagagaaaat
     4981 ttataccaac caacaacttc cgggcaggct ttcacaaaaa aataactgaa acaattctgc
     5041 ggtcgatcag tcaaattcta tatgttctgt tttttgaccg cagtgaatca ttttaaggtt
     5101 gaaatccgta taagatgatt gaggctgatt tttagattat acgtaacggc aattttgagg
     5161 atataatatg ctgactttta ttttatggtg cattctattt gtaatctgct ggccggtggc
     5221 gatcatagcc ctgatactct atccatttat ctggctcctg cttttgcctt tcatgttact
     5281 tggcttcgcc gtcggtggaa tactggcttt cttcaaggcg ctgttctacc tgcccgcccg
     5341 catcatcggc ggcagataat cggaagttat tgaaacatat ttatgactct tcctgttatt
     5401 cccatctggc tttatagcgg ctaagtgcag tggcaatctt gaatattaca gtaatgtatg
     5461 ttcagg
//
LOCUS       DSEB01000137            3474 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002975, whole genome shotgun sequence.
ACCESSION   DSEB01000137 DSEB01000000
VERSION     DSEB01000137.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002975"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..955)
                     /locus_tag="ENO07_02580"
     CDS             complement(<1..955)
                     /locus_tag="ENO07_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00882.1"
                     /translation="MTWNYKICLACLAVSIFAGGPLYAQGMILDFSHDKSTYHWEDSI
                     WIDNDFSDNLRLQMYNHSTATLIKKSLFINSRDRWQKNANTNLALSFNPRNRFSWGIT
                     AFNNYNRLESRRVGINRLGLHQDYKISDAIKVHSQLAYSEATRHYRETKERDRGVMQK
                     LDLTYVGNLFGLGSLGASYNHELNLLRRTPEKSFGLDFGFANNGTEQEIQINYSGNYQ
                     QNRFFTDLTSFENVTTQNKYEHQGDLHINIDLIRNLDLDLVSNYSYRRFEYTEDEDDA
                     LTGIIGRDNLTATFYYKLAGSYPLFGVSLFKTEYIYRDSDEE"
     gene            complement(939..1745)
                     /gene="rsmA"
                     /locus_tag="ENO07_02585"
     CDS             complement(939..1745)
                     /gene="rsmA"
                     /locus_tag="ENO07_02585"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal RNA small subunit methyltransferase A"
                     /protein_id="HDS00883.1"
                     /translation="MLKPKKSLGQNFLKSKKTAQRIVDLLDPHADDVILEIGPGTGVM
                     TEILIESGAKVYAVDIDQRMINFLAQKYARARNLEAVHSDIMDYDFDQIESKERIKVI
                     GNLPYHMTSPILEKICYFHHKIDFAILTVQKEVAARITAEVGSSDYSALTIFINNYCN
                     AEVMFDLGRKQFHPPPEVTSSVIRLDFFKKPLIDPGRYLLIRDLIKKLFSQRRKKVVN
                     SLMNRLNMSREKAQEILDDASIDKNNRPQNISLEEYVRLADALYKYDVEL"
     gene            complement(1745..2506)
                     /locus_tag="ENO07_02590"
     CDS             complement(1745..2506)
                     /locus_tag="ENO07_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family deoxyribonuclease"
                     /protein_id="HDS00884.1"
                     /translation="MIDTHCHLDFENYDDDREEVIRETIEGGVDRMINIGIDESTSIK
                     GLELSEKYENIYASIGYHPHSASEFSKQVQDKLERLAQHPKAVAIGEVGLDYYRNYSP
                     REDQINAFRRQIRLAAEMELPIVVHIREAMEESLKILKEENAGEFGGVLHCFPGSYED
                     SRRALDMNLMVAFGGTITFNNARAAKVAEKVPLKQIVLETDAPFLTPVPFRGKRNSPL
                     YVHYVYKKLAEIKETDFAELEKKIDKNAVSLFNLS"
     gene            complement(2508..>3474)
                     /gene="metG"
                     /locus_tag="ENO07_02595"
     CDS             complement(2508..>3474)
                     /gene="metG"
                     /locus_tag="ENO07_02595"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HDS00885.1"
                     /translation="PDDMIGWFGVDASRFLVLNMCAFGSDGDIRIEDFYTKYNSDLAN
                     NYGNLVSRTLKMIDRNLDGILPQPSGDDMPEIIKDGYETVGKVRDRLVDVQIKMATDE
                     IIAYLSKLNTYFDEKKPWVLAKEGASNELGKVLYNVSYGIALSSALLSPYLTEKTATV
                     LECLFGKKIPPNVVLQELGHALGFSDKIEIPKENLFPRLSMPKELETKAEAEADNLIG
                     IEDFAKVKLQVAEVKAAEKVPGADKLLKLQLEIGRELRQIVAGIAEYYEPESLLGKKI
                     IVVTNLKPVKLRGIESNGMLLAAQKNKKLTLLTVDSDIPSGAKIS"
BASE COUNT          809 a          844 c          849 g          972 t
ORIGIN      
        1 attcttcatc gctgtcacgg tagatatatt cggtcttgaa aagagatacc ccgaaaaggg
       61 ggtatgaccc ggccagcttg tagtagaagg ttgcggtcag gttgtcgcgc ccgattattc
      121 ccgtcagggc atcatcttca tcttccgtgt actcaaagcg gcgataggaa taattggaga
      181 caagatcgag atccagattc cttatcagat caatatttat atgcaggtcg ccctgatgct
      241 catatttgtt ctgggtggtc acattttcaa atgatgtcag gtcggtgaaa aatctgttct
      301 gctggtaatt gccgctgtaa tttatttgaa tttcctgctc tgtgccattg ttggcaaaac
      361 cgaaatccag gccaaaagat ttttcaggag ttctccgcag gaggttcagc tcatgattgt
      421 atgaggctcc caggctcccc agcccgaaga ggtttccaac ataggtaaga tcgagtttct
      481 gcatcactcc ccggtcgcgc tcctttgtct cccggtaatg ccgggtggcc tcggaatagg
      541 cgagctggga gtgtaccttg atagcatcac tgatcttgta gtcctggtga agtcccaggc
      601 ggtttatccc cacccgcctg ctttccagcc ggttatagtt gttaaaggct gttatgcccc
      661 aggaaaaacg gttgcgggga ttaaagctca gggccagatt ggtattggca tttttctgcc
      721 agcggtcgcg gctgtttatg aacaatgatt tttttatcag ggttgcggtg gaatggttat
      781 acatctgcag gcgcagatta tcggaaaaat cattgtctat ccagattgaa tcttcccagt
      841 gataggtcga cttgtcgtgg gaaaaatcga gtatcatgcc ctgggcatac agcgggccgc
      901 ccgcaaaaat tgaaactgca aggcaggcga ggcatatttt ataattccac gtcatattta
      961 taaagcgcgt ccgcgagacg aacatattct tcgagagaaa tattttgggg gcggttgttc
     1021 ttatcgatgg aggcgtcatc caggatttcc tgcgcttttt cccggctcat gtttaacctg
     1081 ttcatgaggg aattgacaac cttcttgcgt ctctggctga aaagtttttt tatcagatct
     1141 cggattaaca ggtatcggcc gggatcgatc agcggttttt taaagaagtc cagccgtata
     1201 acggatgagg ttacctcggg cggggggtga aactgctttc tgccaagatc gaacatcacc
     1261 tcggcattgc agtaattatt tatgaaaatt gtcagggcgc tgtaatcgga actgcccacc
     1321 tcagcggtaa ttctggctgc cacctccttc tggacggtca gaattgcaaa gtctatcttg
     1381 tggtggaaat agcaaatctt ttccaggatg ggggaggtca tatggtaggg aagattgcca
     1441 attaccttga tccgctcctt gctttcaatc tggtcaaaat catagtccat tatatcagaa
     1501 tgcacggctt ccagattccg ggcgcgggca tacttctggg ccagaaaatt gatcatgcgc
     1561 tgatcgatat ccacagcata gacctttgcg ccggattcga tcaggatctc agtcatcacg
     1621 cctgttcccg gaccgatctc cagaataacg tcatccgcat gggggtccag aagatcgaca
     1681 attcgctgcg ccgtcttttt gctctttaag aaattctggc ctaatgattt ctttggctta
     1741 agcatcagga tagattaaac aaagaaaccg cgtttttatc aatctttttt tcaagttcgg
     1801 caaaatcagt ttctttgatt tcggccagct ttttgtaaac atagtgtaca taaagcggcg
     1861 aattgcgttt accgcggaag gggacaggag tcagaaaagg agcatcggtt tcgaggacta
     1921 tctgtttcaa aggcactttt tcggctacct ttgcagcgcg ggcattatta aaagttattg
     1981 tccctccaaa ggcaaccatc aggttcatat ccagcgccct ccggctatcc tcgtaactcc
     2041 cggggaagca gtgcaggaca cccccgaact caccggcgtt ctcctctttc agaatcttca
     2101 ggctttcctc catagcctcg cggatatgca cgacaatcgg caactccatc tcggctgcca
     2161 gccttatctg gcgcctgaag gcgtttatct gatcctcacg gggagaataa ttgcggtaat
     2221 aatcaagccc gacttcgccg attgccacag ctttgggatg ctgggccagg cgttccagtt
     2281 tatcctgaac ctgtttgctg aattcgctgg cggaatgggg atggtaacct attgaggcgt
     2341 aaatattttc atatttttca gagagctcca gcccctttat ggaagtggat tcatcaatgc
     2401 cgatattgat catccgatcc accccgcctt cgattgtctc cctgataacc tcctcacggt
     2461 cgtcatcata attttcgaaa tcgagatggc agtgcgtatc aatcatatca actgattttg
     2521 gctccggatg gtatatccga atcaacggtg agaagcgtga gttttttatt cttctgggcc
     2581 gccagaagca taccgttgga ttcgatcccg cgcaatttta ccggtttgag gttggtgacc
     2641 acaatgatct tcttgcctaa aagtgattcc ggctcataat attctgcgat cccggccaca
     2701 atctggcgca gttcccgtcc gatttcgagc tgcagcttca gaagtttatc ggctccgggg
     2761 actttttcgg cggccttgac ctcggctact tgcagcttaa ctttggcgaa atcctcgatt
     2821 ccaatcaggt tgtcggcttc tgcttcagcc ttagtctcga gctccttagg catggaaagc
     2881 cgtggaaaga gattctcttt gggtatctcg attttatccg agaatcccag cgcatgcccg
     2941 agttcctgca gaaccacgtt gggtggtatc ttttttccaa acaggcattc taaaaccgtc
     3001 gcagtctttt cggtcaggta aggtgaaagc agggcggacg atagagcaat accgtaagag
     3061 acattgtata agacttttcc gagctcattg ctcgcgcctt cctttgccag cacccagggc
     3121 ttcttttcat cgaagtatgt attcaacttg ctcaggtagg cgatgatctc gtcggtggcc
     3181 attttgatct gtacatcaac cagcctgtcc cggaccttcc cgacagtctc atagccgtcc
     3241 ttgataatct ccggcatatc gtcgccggaa ggctggggca ggattccgtc aagattacgg
     3301 tcaatcattt tcaatgtgcg cgaaaccagg ttgccgtagt tattggcgag gtcggaattg
     3361 tacttggtat agaagtcctc gatacggata tcgccgtcgg agccaaaagc gcacatattc
     3421 agtaccagga aacgagaggc atctacacca aaccaaccta tcatatcgtc cggc
//
LOCUS       DSEB01000138            5466 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001078, whole genome shotgun sequence.
ACCESSION   DSEB01000138 DSEB01000000
VERSION     DSEB01000138.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001078"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..419)
                     /locus_tag="ENO07_02600"
     CDS             complement(<1..419)
                     /locus_tag="ENO07_02600"
                     /inference="COORDINATES: protein motif:HMM:PF13649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDS00886.1"
                     /translation="MFYDEISEYYDQMIPVEKRIANLKKQLAPIIKENDIRSALDVGS
                     ATGATCIALAELGVEPVGIELSSKMIKVAREHARRAGMKIKFFKANMLYDQSQFKRRF
                     DAIFVLANTISHLLDKKSLNRAIANFKKWLNPGGILL"
     gene            complement(424..1290)
                     /locus_tag="ENO07_02605"
     CDS             complement(424..1290)
                     /locus_tag="ENO07_02605"
                     /inference="COORDINATES: protein motif:HMM:PF09992.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00887.1"
                     /translation="MGNRYRKFIGNSMILKAITTIFIILCFSGAAASQNEVHIIDSAT
                     GLAMHKYRIVNPQDSAENDLYILKIDPEYYEFQLLAAIELDSVSRTVSQWCAEFGYEL
                     CFNAGMYAADNLTPLGYCKNNGRVLNPHVNYHKAVLAFSPENDSIVPVTIINRGCNDL
                     DSFENRYGSMVQSVRMLGCAGGNVWNQSDKKHSILALATDSAGMVFLLFSQAPSSVHD
                     FINNVKALEQGLEQMIYLEGGIPAALYVRTNNYSFNISGVAEDLWPVRISGSGLTKLP
                     NVIAVKKKADRR"
     gene            1404..1790
                     /locus_tag="ENO07_02610"
     CDS             1404..1790
                     /locus_tag="ENO07_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HDS00888.1"
                     /translation="MPYHISKTVDYSFDEAIQRATDALKTEGFGVLTEIDVKDTLKQK
                     LNADFRDYRILGACNPEFAHQALLKEDKIGTMLPCNIVVQVNPEGQVEVSAVDPMESM
                     SAVQNDSLADIAKQVRERLQRVIDNL"
     gene            1822..2253
                     /locus_tag="ENO07_02615"
     CDS             1822..2253
                     /locus_tag="ENO07_02615"
                     /inference="COORDINATES: protein motif:HMM:PF02566.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HDS00889.1"
                     /translation="MKIVTASLGKKYTTEIHMQEHDLTADEPEKAGGDNIGPNPYDLL
                     LASLAACTAMTIRWYAQRKKLDLTGCEVTLSYDRIHIKDCEACEEGDPRSHIEHIKRT
                     VEISGKFSDEERRKLLSIPGKCPVSKTLKTGVVIEDEVSYV"
     gene            2246..3358
                     /locus_tag="ENO07_02620"
     CDS             2246..3358
                     /locus_tag="ENO07_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00890.1"
                     /translation="MFESIITDLRKVAFAGIILLSPILIFPACGGEEPRQEEQESAAV
                     SEANLINPGEEKYLANVKQLTFGGENAEAYFSYDADKLIFQSTRDSLECDQIYMMNSD
                     GSDVRLVSTGKGRTTCAFIAPDGGKIVYASTHLADPQCPPPPDMSRGYVWALYPGYDI
                     FRADPDGSDLERLTDNPYYDAEAVYSPDGSRLVFTSLRDGDLDIYTMNPDGSDVKRIT
                     SELGYDGGAFFSLDGEWLVYRAHHPRDSAEVADYMYNLSRNLIRPGELEIFIVRSDGT
                     GRKQITDLGGANFCPYFHPDGKRIIFASNHHTGDMNFDLFMINVDGTSLKQITFEADF
                     DGFPMFSHDGKRLVFASNRNQSEPGETNIFIADWQD"
     gene            complement(3486..4139)
                     /locus_tag="ENO07_02625"
     CDS             complement(3486..4139)
                     /locus_tag="ENO07_02625"
                     /inference="COORDINATES: protein motif:HMM:PF12826.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00891.1"
                     /translation="MFDSNLSMIVSNRQKGRTVTTELENLGIVLRYANLKSGDFLITD
                     KIAVEIMTADEFIEAINNKMLFRKLIDFKRDFPEAVFILQGFISLNGKMNSPKIRSAI
                     SYITILNRMPMIMTTDGKDSAQYLNLLVRQAQHGLTFDHENDRKKERYGSLKEAQLAV
                     LSSLPEVGSATAEALLTHFGSLSKVFKATASDLMKVGGIGKKKAERIIKVFEAKYRK"
     gene            complement(4341..>5466)
                     /locus_tag="ENO07_02630"
     CDS             complement(4341..>5466)
                     /locus_tag="ENO07_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HDS00892.1"
                     /translation="QGYKVEWLLNYRAGSFLTPARDDIGEICLLRGVTYEIIPDAEVE
                     NIYRTIEAENMEVILLEKPPEVAIYTPPNKEPWDDAVTLALTYAEIPYTTLWDEEVLD
                     GGLEKYDWLHLHHEDFTGQFGKFYGAYRNAEWYKQEVATNEIAAKALGFRKVPELKLA
                     VAKTVKDYIYSGGFVFAMCSAPETMDIALAAENTDIVPKEYDGDPIDPNCQKKLRYEN
                     CLAFENFTVSLNPLEYRHSDIDTYPERTNYIKNQDMDYFRLFDFSAKLDPVPSMLVQC
                     HLGQIRAFMGQSTAMRKSLLKSHVTVLAEVEGFDEVRYIHGNYGRGTFTFLAGHDPED
                     YMHMVGDPPTDLELHKNSPGYRLILNNILFPAAKKKERKT"
BASE COUNT         1381 a         1313 c         1316 g         1456 t
ORIGIN      
        1 attaacagga ttccaccggg attgagccac ttcttgaaat tagcgattgc gcggttgaga
       61 gatttcttgt ccagaagatg agatatggtg tttgcaagga caaaaattgc gtcaaagcgc
      121 cgtttgaact gagattggtc atacagcatg tttgctttaa aaaatttaat tttcatgccg
      181 gcccggcggg catgttcccg ggctaccttt atcatcttgg aggagagctc aatacccaca
      241 ggctcgaccc cgagctccgc cagagcaata caggttgcac cggtggccga accgacatcc
      301 agagctgacc tgatatcgtt ttcctttatt attggagcaa gctgcttttt caggttggca
      361 attcgcttct cgaccggaat catctggtca taatattcgg aaatttcgtc ataaaacatc
      421 gacctacctc ctgtccgcct tttttttgac cgcaattaca ttgggaagtt ttgtcagtcc
      481 ggagccggaa attctgaccg gccacaaatc ctccgccact cccgaaatat taaaactgta
      541 attattggtt ctaacataga gagcggcggg aataccgcct tcgagataga tcatctgttc
      601 cagtccctgt tcgagcgctt tgacattatt gataaagtcg tgaactgaag acggcgcctg
      661 ggaaaataaa agaaatacca tgccggcgct gtcggtggca agggccagga tgctgtgctt
      721 tttatcggac tggttccaga cattcccgcc cgcacaaccg agcatgcgca cgctttgaac
      781 catgctgccg tatcggttct cgaaagaatc gagatcgtta cagcctctgt taataattgt
      841 gaccgggaca atgctatcat tttcagggct gaaggccagc acagccttgt gataattgac
      901 gtggggattc aaaactctgc cgttattctt gcagtatccc aggggagtca ggttgtcggc
      961 cgcatacatc ccggcattga aacaaagttc atatccgaat tccgcgcacc attgagaaac
     1021 agttcgtgag acagaatcca gttcgatggc ggcaagaagt tgaaattcgt agtattcggg
     1081 gtcaatcttc aggatgtaca ggtcgttttc cgctgaatcc tgaggattta cgatcctgta
     1141 cttgtgcatt gcaagccctg tggctgaatc gataatatgg acctcatttt gcgatgcggc
     1201 tgcccctgaa aagcacaaga tgataaaaat agtcgttatc gcctttaaaa tcatagaatt
     1261 ccctatgaac tttctatacc tgttgcccat tatacgaatg aataactgta tagtaacttt
     1321 atttcagaaa gtaaattaaa atttgtaatt ctcgatgctg aataaatcta ttataatcac
     1381 aagaaaaggg aaggagattt gagatgccgt atcatatcag caaaacagtc gattattctt
     1441 ttgatgaggc catccagcgt gcgacagatg ccttgaaaac agagggtttc ggcgttttaa
     1501 ccgagatcga tgtcaaggac acgctgaagc agaaactcaa tgccgatttt cgcgactaca
     1561 ggattctggg agcctgcaat ccggaatttg cgcatcaggc cctcctgaaa gaggacaaga
     1621 tcgggacgat gctgccgtgt aatattgttg ttcaggtcaa tcccgagggc caggtcgagg
     1681 tctctgcggt tgatcctatg gagtcgatgt ccgccgttca gaatgattcg ctggccgaca
     1741 ttgcaaagca ggtgcgtgag aggcttcaaa gggtcatcga taatctgtaa ttaaatccct
     1801 aactcaatcg ggaggaatcg aatgaaaatc gtaaccgcct cgttaggcaa aaagtacaca
     1861 accgagattc atatgcagga gcatgatctg accgctgacg aaccggagaa ggccgggggc
     1921 gataatatcg gtcccaatcc ctacgatctg cttctggcct cgctggcggc ctgtaccgct
     1981 atgacgatca ggtggtatgc ccagcgcaag aaactggatt tgaccggatg cgaggttacc
     2041 ctgtcgtacg accgaatcca tatcaaggac tgcgaagcct gcgaggaagg cgatccaagg
     2101 agtcatatcg agcacatcaa gcgcacagtc gagatcagcg gcaagttcag cgacgaagag
     2161 aggcggaaac ttctctcaat tccgggtaaa tgtccggtct cgaaaacctt gaaaacgggc
     2221 gtggttattg aagatgaggt gtcatatgtt tgagagtatc ataactgatc tgagaaaagt
     2281 tgcttttgcc ggaataattt tgctttcccc tattttgatt ttcccggcat gcgggggaga
     2341 ggaaccacgg caggaagagc aggaatcagc ggcggtctcc gaggccaatc taataaatcc
     2401 gggggaagaa aaataccttg ccaatgttaa acagttgaca tttggcggcg aaaatgccga
     2461 ggcatatttt tcttatgatg ccgataaact gatttttcaa tccacccggg acagtcttga
     2521 atgcgatcag atttatatga tgaactccga tggctcggat gtcaggctgg tttctaccgg
     2581 aaaagggagg accacctgcg cattcattgc tcccgatggc gggaagatcg tgtatgcctc
     2641 aactcatctg gctgacccgc aatgtccgcc acctccggac atgtcgcgcg gttatgtctg
     2701 ggcgctctat cccggctatg atatttttcg agccgaccct gatggttccg atctcgaacg
     2761 cctgaccgat aatccgtact atgacgccga ggcggtatat tctcctgacg gcagcaggct
     2821 tgtcttcacc tcgctgcgcg acggcgacct ggatatatac accatgaatc ccgacggaag
     2881 tgatgtaaaa agaatcacca gcgagctcgg ctatgacggc ggggctttct tctcacttga
     2941 cggcgagtgg ctcgtgtatc gcgcgcatca tcccagggat tcggctgagg ttgctgatta
     3001 catgtacaat ctttccagaa acctgatccg tccgggagag ctggagatat ttatcgtacg
     3061 cagcgatggg accggccgca aacagataac cgatctgggc ggggcaaatt tctgtcccta
     3121 tttccatcct gatggcaagc ggatcatatt tgcttccaac catcataccg gcgatatgaa
     3181 tttcgatctg tttatgatca atgtagatgg caccagtcta aagcagatca catttgaagc
     3241 tgattttgac ggtttcccga tgttttcgca tgacggcaaa agactcgtct ttgcatcgaa
     3301 caggaatcaa tcggagcccg gggagactaa tatctttatt gcggattggc aggattagtt
     3361 aaacttatcc acggttccca attcagaaac cagaacccct tggttttttt aattgatcac
     3421 tctttttgga aaagaacgca gtcaattatt ccaagctgtc aaacttgaaa caaagtctct
     3481 cagctttact tgcggtactt tgcttcaaat accttgatta tcctttcggc ttttttcttg
     3541 ccgattcctc cgactttcat caggtcagag gctgtcgcct taaaaacctt tgacagtgat
     3601 ccaaaatggg tcaacagtgc ttcagcggtg gccgagccaa cttccggcag cgaggatagg
     3661 accgcaagct gggcctcttt cagggaccca tatctctcct tcttcctgtc gttctcgtgg
     3721 tcaaaggtca ggccatgctg agcctgccgt acgagcaggt tgagatattg cgccgaatcc
     3781 ttgccatcag ttgtcatgat catcggcatg cgatttaaaa tagtgatgta ggagatcgcg
     3841 cttctgatct tgggcgaatt cattttaccg tttaaggaaa taaatccctg gagaatgaaa
     3901 accgcctcgg gaaaatcgcg cttgaaatca atcagcttcc tgaacaacat cttgttgttg
     3961 atggcctcga taaattcatc tgcggtcata atttccaccg caattttgtc ggtaatcagg
     4021 aagtctcccg atttcaagtt agcataacga agtactatgc ccagattctc cagctcagta
     4081 gtgaccgttc tgcctttctg ccggttggat actatcatcg atagatttga atcgaacaat
     4141 ccaagtctcc ttgtaattgg tatattatgc cttttgggct ttagtttaga attaagagaa
     4201 tcgcaaaata tacgttttat tttatgcgac gcaactctaa tttttcttaa atttctgcct
     4261 atattttttc agcaatcagg aagatacaaa tttgtgctgc tgttctttat aacagcagcc
     4321 agaccataaa gtgcctgaaa tcaggtctta cgttccttct tctttgcggc cgggaaaaga
     4381 atgttgttca aaatcaggcg gtaacccgga gagtttttgt gaagctccag gtcagtgggc
     4441 gggtctccca ccatgtgcat ataatcctcg ggatcgtgcc cggccaaaaa ggtgaaggtc
     4501 cctctgccat aatttccatg tatgtatctt acctcatcaa acccttcaac ctccgccagt
     4561 acggtaacat gagatttcag cagcgacttg cgcatggcgg tgctttgtcc cataaaggcc
     4621 ctgatctgcc ccaggtgaca ctgaaccagc atgctcggta ccggatcgag cttggctgaa
     4681 aaatcgaaga gcctgaaata gtccatgtcc tgatttttta tgtaattggt tctttccgga
     4741 tatgtgtcga tgtccgaatg cctgtactcc aggggattta acgataccgt aaaattctcg
     4801 aaggccagac agttttcgta acgcaatttt ttctggcaat tcggatcgat cggatcgccg
     4861 tcatattcct tcggcactat atcggtattc tctgcagcca gtgcaatgtc catagtttcg
     4921 ggagcggagc acatggcaaa cacaaacccg ccgctatata tataatcttt tactgttttg
     4981 gccacggcca gcttgagttc cggaaccttg cggaatccca gcgccttggc agcgatctca
     5041 ttggtggcga cctcctgctt gtaccattcg gcattacgat aggcgccgta aaatttcccg
     5101 aactgcccgg taaaatcctc gtgatgcagg tgcagccagt catacttttc aagtccgccg
     5161 tcaagcactt cctcatccca gagagtggtg taggggatct cggcgtaggt cagcgccaga
     5221 gtaacggcgt catcccatgg ttccttgttg ggcggagtat agattgcaac ctcgggaggt
     5281 ttctccagaa gaatgacctc catgttctcc gcttctatgg tgcggtatat attttccacc
     5341 tcggcatccg ggatgatctc gtaggttact ccgcgcagaa gacagatctc tccgatatca
     5401 tctcgggccg gcgtcaggaa cgatccggcg cgataattca aaagccactc aaccttatag
     5461 ccctgc
//
LOCUS       DSEB01000139            2924 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004349, whole genome shotgun sequence.
ACCESSION   DSEB01000139 DSEB01000000
VERSION     DSEB01000139.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004349"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            103..1185
                     /gene="dsrB"
                     /locus_tag="ENO07_02635"
     CDS             103..1185
                     /gene="dsrB"
                     /locus_tag="ENO07_02635"
                     /EC_number="1.8.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     beta"
                     /protein_id="HDS00893.1"
                     /translation="MAEVKQERLTDIGPPHYQKYLPPVIKENYGKWAYHELLKPGVYV
                     HVAESGDKIFTVRAASPRLVSIDKIRKYADLADKYCDGYLRFTSRNNVEFLLTDESKI
                     DPLVKDLHELGHPVGGLGNSISNIVHTQGWVHCHSAATDASGIVKCVMDELADYFTSM
                     KLPGKLRIALACCLNMCGAVHCSDIAILGIHRRPPRVFDDILPKQCEIPSVVASCPTA
                     AIRPATKDGKKTVEVIEENCMFCANCYTVCPAMPLNDPLNDGVSIWVGGKVSNARHEP
                     MFSKLAIPFIPNNPPRWPEVVDAVKNLVELWAKNARKYERMGEWIERIGWPKFFKMAG
                     IEFQKEHIDDFKFAGLTYKRSTHITY"
     gene            1199..1450
                     /locus_tag="ENO07_02640"
     CDS             1199..1450
                     /locus_tag="ENO07_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00894.1"
                     /translation="MADKLTVDEVADQMYKMVADSQGQKKLKPGDLTKAVKEVYGDKV
                     DRKLCKDAIRSLIESGRCVYTYFGGSYIELPHREGAAND"
     gene            1466..2911
                     /gene="cobB"
                     /locus_tag="ENO07_02645"
     CDS             1466..2911
                     /gene="cobB"
                     /locus_tag="ENO07_02645"
                     /EC_number="6.3.5.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenobyrinic acid a,c-diamide synthase
                     (glutamine-hydrolyzing)"
                     /protein_id="HDS00895.1"
                     /translation="MSLMASKSCPRLVIAGTSGDSGKTLVSLGILAGLRQHGYKVAAF
                     KKGPDYIDPAWLNAASGKSVRNLDTFLVDREKVYRTFARRAIENGINIIEGNRGLYDG
                     LDTHGSHSTAELAKLLNSPIVLVCNVTKSTRTVAGVVLGMREMDRKANIAGVILNKVG
                     GKRHKDIVKDSIESITKIPVLGAIPRVKNEKLLPSRHLGLITPAEYPDFADLIEGLGK
                     LVSDNVDLAALVNIAESAAKLEYEAEGGREKSGSVPAIKIGYFSDTAFTFYYPENLES
                     LQELGAELLPISSLADRELPDIDALYIGGGFPETHAERLAGNRILMDAVKKAALDGLP
                     VYAECGGLIYLCSSLTTGESNYPMAGLFDVDLKMNSSPQGHGYSIMTVDRDNPYYPKG
                     AQLKGHEFHYTSPIRVGRNVNTVMSVSRGSGFEGGRDGLALKAVWASYLHLHASGEQG
                     WSEALVKLASEYKTGKSKSGGGAGVSSRKAV"
BASE COUNT          765 a          652 c          819 g          688 t
ORIGIN      
        1 attctatgaa gagtactatg aagagggtga tgatgacgag gaagaggaag aagaagaata
       61 gtcactggga gaaaataacc ttaatataaa gaggtaaaaa acatggcaga agtaaaacaa
      121 gaaagattaa ctgatatagg tccgccacat tatcagaagt acctgcctcc ggtaatcaag
      181 gagaactatg gcaaatgggc ttatcatgag ctcctgaagc ccggtgtgta cgttcatgtg
      241 gccgagagcg gcgataagat tttcacggtt cgagccgcct caccaagact tgtaagtata
      301 gataagattc gtaaatatgc agacttagcg gataaatact gcgacggtta tctgcgtttc
      361 accagccgta acaatgttga gttcctgctg actgacgaga gcaagatcga tccgctggtc
      421 aaagaccttc atgaattggg tcatccggtc ggcggtctgg gaaattcgat ttccaatatc
      481 gtgcatacgc agggttgggt acactgtcac tctgcggcga ctgacgcctc cggtatagtg
      541 aagtgcgtca tggatgaact ggcagattac ttcaccagca tgaagcttcc cggcaagctc
      601 agaattgccc tggcctgttg tttgaatatg tgcggcgcgg ttcactgctc ggatatcgct
      661 attctgggta ttcaccgtcg cccgccgcgg gtgttcgatg atatcctgcc caagcagtgc
      721 gagatcccga gcgtggtggc atcctgcccg accgcggcga ttcgccctgc caccaaggat
      781 ggcaagaaaa cagttgaagt tattgaggag aactgcatgt tctgcgccaa ctgttatact
      841 gtttgtcctg ctatgccgtt gaacgatccg cttaatgacg gcgtttcgat ctgggtaggc
      901 ggaaaagtct ccaatgcacg tcatgagccg atgttctcga agctggcgat tccattcatt
      961 cccaacaatc cgcccagatg gccggaagtt gttgatgccg tgaaaaatct ggtcgaatta
     1021 tgggccaaga atgccaggaa gtacgagaga atgggtgaat ggatcgaacg tatcggctgg
     1081 ccgaaattct ttaagatggc cgggatcgaa ttccagaagg aacacattga cgatttcaaa
     1141 ttcgctggtc tgacatacaa gcgttcaact cacataacct attaataaag gatatattgt
     1201 ggctgacaag ttgacagttg atgaagtagc tgaccagatg tataagatgg ttgccgattc
     1261 tcaggggcag aagaagctca aaccaggcga ccttaccaag gccgtaaaag aggtttatgg
     1321 cgataaggtc gaccggaaac tctgtaaaga cgctatcagg tctttgatcg aatcgggccg
     1381 ttgtgtttac acctatttcg gcggcagcta tatcgaactt ccgcacaggg aaggcgccgc
     1441 caacgattag gattgagttc ggcctatgtc gttgatggca tcgaaatcct gcccccgcct
     1501 ggtcattgcg ggcacttccg gcgattcggg caagaccctc gtttcactgg gaattctggc
     1561 gggactgaga cagcatggtt ataaagttgc ggcatttaag aaggggccgg attatatcga
     1621 tccggcctgg cttaatgcgg ccagcggaaa atcggtcagg aatctcgata catttctggt
     1681 ggaccgggag aaggtttatc ggacttttgc cagacgggct attgaaaatg gtattaacat
     1741 tatcgaaggc aaccggggac tatacgatgg actcgatact catggttcgc acagcaccgc
     1801 cgaactggcc aagttgttga attcgccgat tgtgctggtc tgcaatgtaa ccaagagcac
     1861 acgaactgtg gccggagttg ttctgggaat gagagagatg gacagaaaag cgaatattgc
     1921 cggagtaatt ctcaataagg ttggcggaaa aagacacaag gacattgtga aagattctat
     1981 cgaaagtata actaaaatac cggttctggg ggcgatcccg cgggtgaaga acgagaaact
     2041 tctgccgtcc aggcatcttg gtctgattac tccggctgaa tacccggact ttgctgattt
     2101 gattgaagga ctgggtaagc tggtatcgga taatgtcgat ctagcagcgc tggtcaatat
     2161 tgccgaatcg gccgctaagc tggaatatga ggctgaaggc ggtcgagaaa aatcgggctc
     2221 cgttcccgcg atcaaaatcg gatacttttc tgatacggca ttcacttttt attacccgga
     2281 gaacctggag tccctgcagg agctgggagc cgaattacta cccatatcat cgcttgcgga
     2341 tagagaactt cctgatattg acgctctcta tataggcggc ggatttccgg agacccatgc
     2401 cgaaaggctg gccgggaatc gcatcctgat ggatgcggtc aagaaggcgg ccttagacgg
     2461 cttgcctgtc tatgctgagt gcggcgggct gatttacctt tgcagcagtc tcacgactgg
     2521 agagagtaat tatcctatgg ccggtctgtt tgatgtagac cttaaaatga atagcagtcc
     2581 ccagggacat ggctacagca ttatgactgt tgaccgcgat aatccgtatt atccaaaagg
     2641 tgcgcagctc aaagggcatg agtttcacta tacctcccca atccgtgtgg gccgtaacgt
     2701 gaatacggtt atgtcggtca gcagaggttc cggatttgaa ggcggccgtg acggcctggc
     2761 actaaaggcg gtctgggcca gttatctgca cctgcacgcc tcgggcgagc agggctggag
     2821 cgaagccctg gtgaagctgg cgtccgaata taagaccggc aagagtaaat cgggaggagg
     2881 cgctggagtt tcttcccgca aagccgttta aaaaggaaaa ataa
//
LOCUS       DSEB01000140            2222 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1008020, whole genome shotgun sequence.
ACCESSION   DSEB01000140 DSEB01000000
VERSION     DSEB01000140.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1008020"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1991)
                     /locus_tag="ENO07_02650"
     CDS             complement(<1..1991)
                     /locus_tag="ENO07_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00896.1"
                     /translation="MNDKDDNFHLDQSAQNLYDQLGGIDDTFRVDESGASLSSLIDLL
                     NQQTRLLDHYRIILADAICLFAEILTGSRSAVSSQVIDQKIHAFLADLKKIAEFPQFN
                     RKIYCRLRGQQYTSDQSDSEIYDYELSFNNILLDCQMARIIEERIKKGSGLYAKLMDA
                     FQSLSSKNIFNLSIDIGAGKQEDYRKLETSLRTLIKFYENPDREGFVVLDEYGQSNIN
                     LTLLAALNKVKPIALQELVDKIKPSIIGPEPDKGLALFTTVYDAIVASLDRRKNLKKI
                     PVEVNNTQLFMENLHADPQRSAVVVQISRLILAKYGSSPRMASEVINSINSAGYINIR
                     PEVMGRRLSRASDFLKLAEQSVNRENLQSEALRNIEEGLDQLPDAMYENISVKGAEAS
                     TFDSEGQETTFVLHYKILDFLSFFIRRSSIKKKVHDIANKNVWFDDDDYAVIAKDFSI
                     SKDDAVKLISLLHDCFDNNGNFRQNFFEKNIQGFANFGIKVFEFLWHYLKELPARNDR
                     VAFLNALQNLVAFFEQPKPALDILLSDIFDRSATNVQFSDRNAFILATSLLCYNIDQY
                     SPRSHIELTPEDVLHARDELNPDTIATVKEFFEPRHENIIQKFRKITQLLLKASAGEH
                     FAENETPRFLLYLLREMVIFFSLIGGQTAHAIIRGVVLE"
BASE COUNT          594 a          506 c          518 g          604 t
ORIGIN      
        1 aactccagaa caactccacg tatgatggcg tgagctgtct ggccgccgat cagggaaaag
       61 aaaatcacca tctccctgag aagataaagg agaaagcggg gcgtttcgtt ctccgcaaaa
      121 tgttccccgg ccgatgcttt cagcagcagc tgagtaatct ttcgaaattt ctggataata
      181 ttttcatggc gtggttcaaa aaattccttc actgtagcga tcgtgtcagg attaagctcg
      241 tccctggcat gcagaacatc ttcaggggtc agctcaatat gacttcttgg cgaatactga
      301 tctatattgt agcacaacag agatgttgcc agaataaagg cattgcgatc agagaactgt
      361 acgtttgttg ctgaacggtc aaatatgtcg gaaagaagta tgtcgagcgc cggcttcggc
      421 tgttcgaaaa aggcaaccag gttctggagc gcgttcagaa aggcgacccg atcatttctt
      481 gcgggaagct ctttaaggta atgccagaga aattcgaaaa ccttgatgcc aaaattggca
      541 aaaccctgga tgttcttttc aaaaaagttc tggcggaaat taccgttatt atcaaaacag
      601 tcatgaagca gacttatcaa tttgaccgca tcatctttgg agatcgagaa atctttggcg
      661 ataaccgcgt agtcatcatc atcaaaccag acatttttat ttgcaatgtc atggaccttt
      721 tttttgatcg aagacctgcg aataaaaaaa ctgagaaaat ccaggatctt gtaatgtaaa
      781 acaaacgttg tctcctggcc ttcgctgtca aaagtgctgg cttccgcacc cttgacggaa
      841 atgttttcgt acatcgcatc aggcagctga tccaaccctt cctcaatgtt gcgcagagct
      901 tcactctgta aattttcccg attaacggat tgttcggcaa gtttcagaaa atcagatgcc
      961 ctgctcagtc tccggcccat cacttccggg cggatattga tataaccggc gctgttgata
     1021 ctgttaataa cctctgatgc cattctgggg ctgctcccgt atttggccag tatcagccgc
     1081 gagatctgga ccaccacggc cgagcgttgg gggtcggcat gcagattctc catgaacaac
     1141 tgggtgttgt taacctcaac aggaattttc ttgaggttct tgcgccggtc cagggatgct
     1201 acgatcgcat cataaaccgt tgtgaaaagg gcaagcccct tatccggttc cgggccaatg
     1261 atcgagggtt taattttgtc aacaagctcc tggagagcta tgggttttac cttgttcaat
     1321 gccgccagca gggtcagatt aatgttggat tggccatatt catcgaggac aacaaatcct
     1381 tcccgatcag ggttctcata aaatttgatc agggtccgca gactggtttc cagcttgcgg
     1441 taatcctctt gtttgccggc gccgatatcg atggatagat taaagatatt cttcgagctt
     1501 aaggactgaa aagcatccat caactttgca taaaggccgc tgcctttttt tatcctttct
     1561 tcaatgattc tggccatctg gcagtcgaga agaatgttgt tgaacgacaa ctcatagtca
     1621 tagatctcgg aatccgactg atccgaagtg tattgctgtc cgcgaaggcg gcaatatatc
     1681 ttacggttga actggggaaa ttccgcaatc tttttcagat ctgccagaaa agcgtggatt
     1741 ttctgatcaa ttacctggga agagacagcg gaacggctgc ctgtgagaat ttcggcaaac
     1801 aggcatatag catcagcgag gattattcga taatgatcaa gcagtcttgt ctgctgatta
     1861 aggagatcaa taagcgatga cagcgaagct ccggattcat ctacccggaa agtatcgtct
     1921 atacctccca gttggtcata gaggttctgg gctgattggt ccaggtggaa attgtcatcc
     1981 ttatcgttca taaatttcct caagataaca aggaggcatt gttattcccc tccccccata
     2041 aaaaaaggac atttcaggac attttgtcaa gacaatataa atatccgagc ccaaagggct
     2101 cggttttgga tggcccttca agggccgagt ttaaaatctt ttcccattgt cgtttgccga
     2161 agcaccgcag gtgttgccgg taaaaaatct gctcttcaaa ttgcagattt tcggcaacac
     2221 cg
//
LOCUS       DSEB01000141            2429 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1006579, whole genome shotgun sequence.
ACCESSION   DSEB01000141 DSEB01000000
VERSION     DSEB01000141.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1006579"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            228..1181
                     /locus_tag="ENO07_02655"
     CDS             228..1181
                     /locus_tag="ENO07_02655"
                     /inference="COORDINATES: protein motif:HMM:PF09699.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00897.1"
                     /translation="MGHKLLSKYLTGAAVLIPLLIFSISYGQYGNKDDILEQTETCLL
                     CHDGQAETLRGTPHQLLSGDDLSSPIMMGCTGCHTDWEDHVDDPAAENIVAGPELGSL
                     EQSQQCAGCHQTPHQAAMATTDPHGRGGYNCSSCHTVHGNQNRMLVKDEQENYCLECH
                     NVVEAQFKARSAHPLQSGNIGCTDCHQFGGIADQLHAQGLNWTCQECHPLIAGPHIYE
                     HPVTYEYLVEGSGCTECHNPHGSANDRLLKQPGDGTCRQCHMVPPGHRIAHAGLGSRH
                     ACIDCHSEIHGSNSNELFLDPDLGIKNVINCYASGCHSLGS"
     gene            1193..>2429
                     /locus_tag="ENO07_02660"
     CDS             1193..>2429
                     /locus_tag="ENO07_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00898.1"
                     /translation="MKKLFIVMIALLFPSLALAASGAGDFSISYLFLDEEGNQSVQHS
                     SFNIYEGPGISIEDFRYTLDNGLSFNADMKNIILNNRNMRLGIGKSGLFGLRLSHNQY
                     RRVYNFDGSSFTRRHTNQGLLWLYPHRHLKLWGGGTYIGRSGSMTDLFDPDPGAISTD
                     VDYDQVQYKGGFQLNYEGGLVRGEFRVVEFDDNENEDRDQTRSEVRFDGLIPVPNYEW
                     IVLSGGFRHFETKYDISDFKISSNRGWGGFRANLPENFQLRYNFIFDRTSSDSDFVAT
                     DNITNAAYVSHVWPQFAMLTVGYHHGINDDFEDEVKSNSFYASGWIRPADGLEIRGEF
                     GLTEEEVKDGSRLIGDESRNRHKFRLKYNSGEYGTIALKYENKIRENDQLGTEIDFIS
                     FGADYSIGVAEYGTLSAGYV"
BASE COUNT          634 a          580 c          617 g          598 t
ORIGIN      
        1 ctttttggat tgcattatta taattgttaa aaatagcgac ggatgtataa agggttgggg
       61 cttagaatat gttcagcgaa catccgcaag aatgagagag gaaacaaaaa tttagtagaa
      121 tcgccctttg aaaaaaaatt tttgctggac ttaattaatg tttattcatt taataaacat
      181 tatcgtataa agtaaataaa cgcattatta aaggattggg agattaattg ggacataagc
      241 tactatccaa gtatctaacc ggagcggcgg ttttaattcc acttttaatc ttttccatat
      301 catatggcca atacggcaat aaggatgata tcctggagca gactgaaacc tgccttctgt
      361 gccatgatgg acaggccgaa acccttcggg gtactccgca tcagttgtta tctggagatg
      421 atttatcctc tccgataatg atgggctgta ccggatgcca taccgattgg gaagatcatg
      481 tggatgatcc tgccgcggaa aatattgtag ccggaccgga gctcggttcg ctggaacagt
      541 ctcagcagtg cgccgggtgc caccaaacgc cgcaccaggc cgcgatggca accaccgatc
      601 cgcacggccg gggcggttac aattgcagtt catgtcatac ggttcacggc aaccagaatc
      661 gtatgctggt caaagacgag caggaaaatt actgcctcga atgccacaat gtagtcgagg
      721 ctcagttcaa ggcaagatca gcccatccgc tgcaatcggg caatataggc tgcaccgatt
      781 gccaccagtt cggcggcatc gctgatcagc ttcacgccca agggttgaac tggacctgcc
      841 aggaatgcca cccgctcata gccggaccgc acatatacga acatccggtg acctatgagt
      901 atctggtcga gggttcgggc tgtacggaat gccataatcc tcacggatcg gcaaatgacc
      961 gtcttttgaa acagccgggg gacggcacct gcaggcaatg ccatatggtc cctccgggac
     1021 acaggattgc tcatgccggc ctgggttcaa gacatgcttg cattgactgt cacagcgaga
     1081 tacatggctc caacagcaat gagctttttc tcgatccgga tctgggtatc aaaaacgtca
     1141 ttaactgtta tgcctctggc tgccacagcc tgggaagttg aggagggatt gtatgaaaaa
     1201 attatttata gtcatgatcg cccttctttt cccctcgctg gctctggccg catccggagc
     1261 gggtgatttt tcaatcagct acctgtttct ggatgaggag ggcaatcagt cggtgcaaca
     1321 ttccagcttt aatatttatg agggtccggg catctcgatc gaagatttca ggtacacgct
     1381 tgacaatggc ctgagcttca acgccgacat gaaaaacatt atcctgaata atcgcaatat
     1441 gcgtctggga atcggcaaat cgggcttgtt cggattaaga ctgtctcaca atcaataccg
     1501 ccgggtttac aatttcgacg gctccagttt taccaggcgg catacgaacc agggcctgct
     1561 gtggctgtat ccgcatcggc acctcaaact ctggggaggc ggcacttata ttggcaggtc
     1621 aggctccatg accgacttat tcgatcctga tccgggcgcg atttcaactg atgttgacta
     1681 tgaccaggtc cagtacaaag gcggcttcca gctcaattat gagggcggcc tggttagagg
     1741 cgaattcaga gtagtcgaat ttgacgacaa tgaaaatgaa gatcgcgatc aaaccaggag
     1801 cgaggtgcgc ttcgacggcc tgatcccggt cccgaattat gaatggatcg ttctttccgg
     1861 tggattcagg cacttcgaaa caaagtatga tatttccgat ttcaagatca gttccaatcg
     1921 tggatgggga ggcttccgcg ctaacctgcc cgagaatttt caattgagat acaatttcat
     1981 cttcgaccgc accagttctg acagcgattt cgttgccacc gacaatataa cgaacgctgc
     2041 ttatgtcagt catgtctggc cgcagttcgc catgcttacg gtcgggtatc atcatggtat
     2101 caacgatgat tttgaggatg aggtcaaatc caattcattc tacgcttccg ggtggattcg
     2161 gcctgcagac ggacttgaaa tcaggggcga gttcgggttg actgaagaag aggttaaaga
     2221 cggctcgcgg ctgatcggcg atgaatcccg caaccgtcat aaattccgcc tcaaatataa
     2281 ttccggcgaa tacggtacga tcgccctcaa atatgaaaat aaaatccgcg agaacgacca
     2341 gctcggcaca gagatagact ttatcagctt tggcgccgac tacagtatcg gggtagctga
     2401 atatggcacc ctcagcgccg gttatgtga
//
LOCUS       DSEB01000142            2222 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1008024, whole genome shotgun sequence.
ACCESSION   DSEB01000142 DSEB01000000
VERSION     DSEB01000142.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1008024"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..742)
                     /locus_tag="ENO07_02665"
     CDS             complement(<1..742)
                     /locus_tag="ENO07_02665"
                     /inference="COORDINATES: protein motif:HMM:PF08486.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00899.1"
                     /translation="MGVRRARQSAGRYRSIKTDSAWEVNSAMRIMRIFIVLLLGLILS
                     GCSRLSYIDRHTGTEMVPHPHIRVRILQAGEVEIDCEGTYRLNCLTADSTSRDYYSVS
                     PLKLVYHDDGLVLSESDGLILDRNLLKIYVSPKVRGSHVVLNGRQFRGVMEIRAAEED
                     IEVINLLNVEDYLKGVLPPEIGRLEEDAFEALKAQSVAARTYAYSRIAYNSDKDYDLV
                     NDIMDQVYIGIKGEYGLANEAIEQTRGEI"
     gene            complement(658..1827)
                     /locus_tag="ENO07_02670"
     CDS             complement(658..1827)
                     /locus_tag="ENO07_02670"
                     /inference="COORDINATES: protein motif:HMM:PF03702.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDS00900.1"
                     /translation="MHPIKKLLSKKDVNIIGMMSGTSLDGLDLSYCRISHNATRHGVK
                     IIKTGYFRFDPVLKRNLQALVSEGRFTKSFIKQADDDLGQFYAASVKRFMKKFGIRPV
                     DMLGCHGQTILHLDKRLSKSKTGRSATWQIGSSSRLASITGLPVVSDFRSADVALGGS
                     GAPLTPLCHYHLFGQDDRNIAVLNIGGITNLTFLPEKSGRKKIRASDCGPGNMLVDQL
                     TKMLFGRDYDKGGRIASTGVISKRLLGSLKRRTWFTGPHPKSLGREQFGADEAMRIIE
                     RGRRYGISNRDIITTVSELTVISVLNYIENLPAVDKLIVCGGGASNNYFMTRLSNLIP
                     RCAVISSEEAGLDPDYVEAACFALLAALWVFGEPANLQAVTGASKQTLLGRLTLP"
     gene            complement(1799..2221)
                     /locus_tag="ENO07_02675"
     CDS             complement(1799..2221)
                     /locus_tag="ENO07_02675"
                     /inference="COORDINATES: protein motif:HMM:PF00933.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00901.1"
                     /translation="MHWKPFEMAIGADVDLFMTTHITAKSLDPENIATFSKEVVSNIL
                     RSKLGFEGVIITDDLDMGAVSKNFPPEECAPAALKAGHDMILICHNKKDQETAFNSVL
                     ESYNNGQIDRDEIAARIKRVKKLKEKLTNHAPYKKASV"
BASE COUNT          547 a          582 c          506 g          587 t
ORIGIN      
        1 gtatttcccc gcgggtctgc tcaatggcct cattggcaag accatactct ccctttattc
       61 caatatagac ctggtccatg atatcgttaa ccaggtcata atctttatct gaattataag
      121 ctatgcggga ataggcgtag gtacgggctg ctaccgactg agccttgaga gcctcaaaag
      181 catcttcctc caaacgccct atttccgggg gcaggacgcc ttttaaatag tcttcgacat
      241 ttaacagatt tatgacctca atatcttctt cggccgccct tatctccatg acgcccctga
      301 actgcctgcc attaaggaca acgtgtgacc cccgaacttt gggagaaaca tatattttga
      361 gaagattccg atcgagtatc agcccgtcag attcagacag caccagacca tcatcatgat
      421 agaccaactt caaaggcgac accgagtaat agtcccttga ggtgctgtcg gccgtgaggc
      481 agttgaggcg atatgtcccc tcgcagtcga tttctacttc gccggcctga aggattctta
      541 ccctgatatg cgggtgggga accatctctg ttccggtatg cctgtcgata taggagaggc
      601 gcgaacagcc cgatagaata agtcccagca gcagaactat aaatatacgc attattctca
      661 tggcagagtt aacctcccaa gcagagtctg ttttgatgct cctgtaacgg cctgcagatt
      721 ggcgggctcg ccgaacaccc ataatgccgc cagcagggca aagcaggcgg cttcaacata
      781 atcgggatcc aaacccgctt cttcactgct tattaccgca catcggggaa taagattcga
      841 gagccgtgtc ataaaataat tattactcgc tcctccgccg catacgatga gcttatcgac
      901 agcaggcagg ttctcgatat aatttaggac tgagataacg gttaattctg agaccgtagt
      961 gataatatcc ctgttcgaaa ttccataacg cctgccccgc tcgataatcc tcatcgcttc
     1021 atcagcccca aattgttcac gtcccaggga tttcgggtgt ggaccggtga accaggttcg
     1081 gcgtttcagc gagcccagga gtctttttga gattactccc gtcgaagcga tcctcccgcc
     1141 cttatcatag tcccggccaa aaagcatttt agttagttga tccaccagca tattgcccgg
     1201 accgcagtcc gatgctctga tttttttcct tccgcttttt tcaggaagaa aagtaaggtt
     1261 cgtaataccg cctatattca gaactgcgat atttctgtca tcctgtccga agaggtgata
     1321 atggcagagc ggtgtcaggg gagctccaga gcctcccagg gctacatcag cagaacggaa
     1381 atcagatacg accggaagac ccgttatcga ggcaagccgg gagctactgc cgatctgcca
     1441 tgtcgcactt ctgccggttt tagatttgga gagccgctta tccagatgca atatagtctg
     1501 gccatgacat cccagcatgt caacaggcct gatcccgaat ttcttcataa aacgcttcac
     1561 gctggcggca taaaattgac ccaggtcatc atcagcctgc tttataaagg atttcgtgaa
     1621 tctgccctcg gatacgagag cctgcagatt tcttttcagc accggatcga aacgaaaata
     1681 tccggttttt attattttta caccgtgtct ggttgcgttg tgagagattc gacaatatga
     1741 cagatcgagg ccgtcaagag aggtccccga catcattcca atgatattga cgtctttctt
     1801 agacagaagc ttttttatag ggtgcatgat ttgtcagctt ttccttaagc tttttcaccc
     1861 gcttaatcct ggcggctatt tcatccctgt caatctgacc attattataa ctttccagca
     1921 ccgaattaaa agcggtttcc tggtcctttt tattatggca aatcagaatc atatcgtgcc
     1981 ctgctttgag ggccgccggc gcgcactcct cgggaggaaa gttcttgctg accgcgccca
     2041 tatccaggtc gtcggtgatg attacgcctt cgaatcccag tttgctgcga aggatattgg
     2101 agacgacctc cttcgagaag gtggcaatat tctccggatc aagcgatttt gccgtaatgt
     2161 gagtcgtcat aaacagatca acgtcagcgc ctatggccat ctcaaacggc ttccagtgaa
     2221 ta
//
LOCUS       DSEB01000143            2561 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1005859, whole genome shotgun sequence.
ACCESSION   DSEB01000143 DSEB01000000
VERSION     DSEB01000143.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1005859"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(361..1677)
                     /locus_tag="ENO07_02680"
     CDS             complement(361..1677)
                     /locus_tag="ENO07_02680"
                     /inference="COORDINATES: protein motif:HMM:PF14344.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HDS00902.1"
                     /translation="MLKSKLFFGLTAMALSFVFLIGCDDDDNAINSNPATTSVRVIHA
                     SYDAPAVDVRVDGSVAITNLAYGESSGYAQLNTGSRNIAVTPAGSTTPVVINADLELA
                     ENTEYTVYAVNGLNSIEPIISVDDRSGRADKAQIRFIHASPDAPAVDIKLNSGTGSTV
                     FSDVAFKDIEDYAAVDQGSYVFAVTPAGSSSEVVVFNPVEVQNGNLYTVIALGTLDAG
                     DQYPFVVRVFVDNGNGNASLDLSAGSSNVMVIHASPDAPAVDLLVDDFKVNTSGLPFP
                     QNTGYLALSAGDRNFKVNAAGTSTTVIDATATLVPNGNFSVFAVDVLANIMPLVLADD
                     LSAPAAGKAHVRFLHLSPDAPEVDITLPDGTIVFGQYEFKESSAFTPLDAGMYDLEVR
                     VSGTPTVALNLDNINLESGKIYTVFARGLLSGSGDRALGAEIIVNN"
     gene            1912..>2561
                     /locus_tag="ENO07_02685"
     CDS             1912..>2561
                     /locus_tag="ENO07_02685"
                     /inference="COORDINATES: protein
                     motif:HMM:PF01593.22,HMM:PF13450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00903.1"
                     /translation="MALMTGYSGTDMFSATFMSIRSGSEGYEELPKKIPIDRCLIIGA
                     GISGLVAGRKLQDQGIDITVIDKGRGVGGRASTRIINGARFDHGAQSFTADDPRFREL
                     VEGFADLGAARVWYEGWPDEKDSEIKNRRVHYYGTNGMNHIPKSLAAGLDVITGEKVT
                     EICFEKNLWKIVTSAGGNFKAPALIMTPPMPQTLQLLSQCEFKLDPDTNRQLSKIS"
BASE COUNT          708 a          577 c          635 g          641 t
ORIGIN      
        1 caatcacaga aggatgtatg gcaggcccag ctggctcagc tccaggtaca ggccgatacc
       61 caggatgaac agaaccggga tgcctattac gagatgatcg acaatcttca gaagatgctc
      121 ggcaatttca atgagaagtt cgatgcgctc aagaaatcgg ttgaggatga ttcctggaaa
      181 gacgttaaaa aggaaatgga agagcttttg aaggcgacca atgacgcttt cggcacggtg
      241 accgcgagat tcaaatccga actttcggat taaaattcag gatttgttat tacaacaaat
      301 gcaggaccgg attgatacgc aatccggccc tttgatttat ctgaaaatcg tagtgatttt
      361 tcagttatta actatgatct cggccccgag agctcggtcg ccggaaccgc tcaatagccc
      421 tctggcgaat acggtataaa tcttaccgct ttcgagattg atattgtcga gattcagggc
      481 gactgtggga gtaccggaaa ctctgacctc gagatcatac atgcccgcat caagcggggt
      541 gaaagccgag gactctttga attcatactg gccaaataca atcgtgccgt caggtagagt
      601 gatatcgacc tccggtgcgt ccggtgagag atgcaggaag cgtacatgag ccttaccggc
      661 ggcgggcgcg ctcagatcgt ctgccagcac caggggcatg atgtttgcga gcacatcaac
      721 tgcgaaaact gaaaagtttc cgttcgggac aagcgtggcg gttgcatcaa taactgtcgt
      781 gctggtgcct gcggcattaa ccttgaaatt cctgtctccc gccgaaagcg cgagatagcc
      841 cgtattctga gggaagggca atccgcttgt attcactttg aaatcgtcaa ccaggaggtc
      901 gaccgcaggg gcatcgggag atgcatgaat aaccattacg ttagaagaac ctgcgctgag
      961 atcaagggac gcattaccgt tgccgttgtc aacaaatacc ctgacaacaa acggatactg
     1021 gtcaccggca tccagagtac cgagagcaat aacagtataa agattcccgt tctggacttc
     1081 gacaggattg aataccacta cctcgctgct gcttccggcc ggagtaactg cgaatacata
     1141 tgaaccctga tcaacggcgg cataatcttc aatatccttg aaggcgacgt ccgaaaacac
     1201 ggtactgccg gtaccgctgt tcagcttgat atccaccgcc ggtgcatccg gagaggcgtg
     1261 aataaacctt atctgtgctt tatccgcacg cccggaacga tcatcgacac tgattattgg
     1321 ctcgattgaa ttcaaaccat tgacagcata gacggtatat tcagtatttt ctgctaattc
     1381 caggtcagca ttgataacta ccggcgtggt tgaaccggca ggtgtaactg cgatattgcg
     1441 gctgcccgta tttaactggg cataaccgga ggattcccca taagccagat ttgtgatcgc
     1501 cacgctgcca tcaacacgaa catcaacagc gggagcgtca tagctggcat gaatcaccct
     1561 gacacttgta gttgcgggat tggaattaat tgcgttgtca tcatcgtcac atccaattag
     1621 aaagacaaat gacagagcca ttgcggttaa tccaaagaat aattttgatt taagcatttt
     1681 tgcttctcct ttttaaagaa tgagtttatt tttatttctg ccggttttaa catatagtgt
     1741 ctaagtaatg ttcccatgat gtcaatattt ttgtaaataa attatgctgc gcctctgtga
     1801 ttgtcataac ctgctattat attggcagtt aaataaaccc agaaccggat atgcgggata
     1861 gaattaggct atttaatatt agatatttat tagacaataa ggcgatatcc tttggctttg
     1921 atgacgggtt attcaggaac agacatgttt tcagcgacgt ttatgagcat aagatcaggt
     1981 tcagaaggat atgaggaatt gcccaagaaa atccctattg acagatgttt gattatagga
     2041 gcaggaattt cgggactggt tgccggacgt aagcttcagg accaaggtat tgatattact
     2101 gtcatcgata aaggccgggg agttggcggg cgagcatcca cacgaattat taatggcgcg
     2161 cgttttgatc atggcgccca gtcatttacg gctgacgatc cccgcttccg ggaactggta
     2221 gaagggtttg ccgacctggg cgctgcccga gtatggtatg agggctggcc tgatgaaaag
     2281 gactccgaga taaaaaacag gagagttcat tactatggaa cgaatggcat gaaccatatt
     2341 cccaaatccc tggcggccgg tctggatgtt attaccgggg aaaaagtaac tgaaatctgt
     2401 ttcgagaaaa atctctggaa aattgtcaca tcagcagggg gcaattttaa ggcgccggcc
     2461 ctgataatga caccccccat gcctcaaact cttcagcttc tcagccaatg cgagttcaag
     2521 cttgatcccg atacaaatag acagttgagc aagatctctt a
//
LOCUS       DSEB01000144            2502 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1006172, whole genome shotgun sequence.
ACCESSION   DSEB01000144 DSEB01000000
VERSION     DSEB01000144.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1006172"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..360)
                     /locus_tag="ENO07_02690"
     CDS             complement(<1..360)
                     /locus_tag="ENO07_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HDS00904.1"
                     /translation="MERSVNMPAFNPFQMARSQFERVAHQLELEDSVRELLSTALREY
                     RFALPVRMDDGSLRVFRGFRVHHNDALGPCKGGLRFHPEVSIDTARAQAMWMTWKCAV
                     AEIPLGGANGGVICDPHN"
BASE COUNT          651 a          647 c          600 g          604 t
ORIGIN      
        1 gttatgagga tcgcagataa ctccgccatt ggcgccgccc aacggtatct cggctacggc
       61 gcatttccat gtcatccaca tcgcctgcgc gcgggcagtg tcgatggaga cctcggggtg
      121 aaatcgcagg ccgcctttgc acggccccag ggcgtcattg tgatgcactc ggaagccccg
      181 gaagaccctc agggaaccgt catccatcct gaccggcagc gcaaatctat attctctcaa
      241 tgctgtggaa agaagctctc gcacagaatc ctcaagttca agttgatgag ccaccctctc
      301 aaattgagag cgggccatct ggaagggatt gaaggcaggc atatttacag atctctccat
      361 tgaaaagtaa agtgtctcga tttaaaccgt tgaccgcccc tggatagatt caaattagat
      421 ccgcgcagaa tcatcctgtt gaagctgtca aacaaatatc tcagcatcga gcaaaatgta
      481 gctcacatta ataaaactgt caaggtgatt atctgtcggc agatttgact tcagtggcaa
      541 tcaaaacagg gcgcagataa gtgcgccctg cattacagga acgtgaaaat gccgctcgga
      601 gccccatagc ggcaacgtaa attaaaaaaa ttttgttaga gatatatttt cttatggtga
      661 gtaatattga tctaatgtac aaaaaagccc gctcgataga atcaagcggg cttttatttc
      721 tatacaatcg tttaaatcta acagtcaggc tcgccgtcac cgtcaggatc tccgggagga
      781 ttaccgccca caaaaacata gttaacgatc atgaccgcgt ctgaaacatt aacagtgccg
      841 tcacagttgg catcgcccgc ggcctcctcc ggaacaggcg ggtcgcctcc gacaaagaca
      901 taatttatga tcgctaccgc atcgctgaca tttatactgc cgtcgaaatt ggcatccccg
      961 acaatgtaat ccggggcaac attgatcggt atgagagggg aggagtaata agcagcatca
     1021 ttcaatttaa gctggattgt gaaatcggtc tccccgggct ggatggcagt gatttgaaac
     1081 gaccagttgc ccgttaattc cgccgacgca atggtggctt cggaaactgc gacatccatg
     1141 gaataataat ttgtatccgg ttgataaatc tgctcctgat catccataaa ccagacctca
     1201 tattcgggag aaatattccc ctcggtcagt tctatagacc cggaaattat gtcattttca
     1261 gccgatatta ttatgctggt tgagtcatac acctgcagac cgctcacggc aacctcttcg
     1321 tagcactgaa tctgggtaac tttgaaggca tcaactcccg cctccacaat gctctgcgga
     1381 tcagcgtcat tggcggtgaa acgtatctgc atgttatcac tcaaggccac tcccgcggcc
     1441 gccagatcca gttctgtgat ctcattatgg ttccagtcca gtccgttgtg ttcatcatgc
     1501 accgcaattg tggtccagag accggcccca ccgctattat tgatttccac caggagctta
     1561 tccacgccgc cctcggtatt ggtcagaaaa agataatagt tataggaaat cgtacccggc
     1621 agcgacatat cgaatatcgg ggatattaaa cggaccgcgc cgttatcaac atcggtattt
     1681 ccatattgat tttgggtcag gtagcacatc ccgctgccat cggcatccga agccggatca
     1741 tattcccagc cgtcgtcatc gacaggcacc cctctttccc agtatccgct ggtcgctccc
     1801 acaattgccg tggtccagcc ctggtcggtc tggaaattat catctatgga ataggccaga
     1861 tttgtagcgg ctgccgcgga atggggaaca tcagtatcat aaaagcggcc gttttcctgc
     1921 tcgtctgcgc cgacataaaa tgccactctc gttccacagg ccgtggctgg aagtgtagca
     1981 ctatataaat tgggcagagt ctcaaccata tccgtgctgt cgaaaggacc tcctacaatt
     2041 gaatagaaga gcttgcccga tccgggaacc ggtattccgg cgttcagacc ataaacctcg
     2101 acactgaacg tagtcggctg gtctggaact aggccctcag gaagaccgcc cgggaagttg
     2161 aaggcaacct ggggctgagc atatgtctgg cccacaacat aaaccgtcgc cagcgccgct
     2221 ttggtcatgt cagtcatata tgagaagttc atatatgtgg tgctgtcctg ggaactgtgg
     2281 taaacggtcg agaatatacc ctcgtgcagg aacgtcgcct catagccatt ctgaataaag
     2341 ggatgatggt ctgagccact caatgatccg gaataaattg aacttatgcc gaccagagaa
     2401 tcggccaggt cggcacaaag gtcggtgtat gtcagaacgg gcccataata taatttgcat
     2461 gtattggtat tctcgtaatg cgctatcatg tccatgttga ac
//
LOCUS       DSEB01000145            3272 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003399, whole genome shotgun sequence.
ACCESSION   DSEB01000145 DSEB01000000
VERSION     DSEB01000145.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003399"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1326
                     /locus_tag="ENO07_02695"
     CDS             <1..1326
                     /locus_tag="ENO07_02695"
                     /inference="COORDINATES: protein motif:HMM:PF12770.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HDS00905.1"
                     /translation="YLGLIECLLKMGRVEESFFENSRALAIINQKIPFTKTRQNKISQ
                     EYLHTRDNLRARLKELSRLPGSGERRADSPRTMKKAEQELWQMERKIRSIIYPSEVNF
                     SYASLPENYYQGFLGDDELLLNYVAIGEDLGVYRVRREKTEYMPLGISRTQMTSLIGE
                     LHFLMEKSIYSASESASLMHIIEDYQRRLYEVLIKPIKPGTGVGKMIFMIDGFFAQIP
                     YWALLDSKGVTLYQRYNLRFISNPVDLKERKSLLPYKISGSSAIFVAENSGLPMVNAE
                     GKRIKEHFENAELFGGADASCGKLLNSLGRADGFVHIATHASRASENPLFSRILMSDG
                     PFFPFDLFGAKINARLVTLSGCQTAAPGVFYGNSFSLAKAFYQAGARYVLASLWPISD
                     KTGMVFMDEFYAELRKHEDIFGAYHQALEKTRELNLNPAFWSPFVLLGI"
     gene            1357..>3272
                     /locus_tag="ENO07_02700"
     CDS             1357..>3272
                     /locus_tag="ENO07_02700"
                     /inference="COORDINATES: protein motif:HMM:PF00082.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00906.1"
                     /translation="MRRHIIILAVIIISAGAFIPGGAYGQDSLFSSCGVLIEGDQCVL
                     FSPQGGGFEAALQFYGDFQPDDSVYVSGIILFNCDTLCAGTDVCIVNNSIGSCDGDPY
                     EPFDGPGVLVQTSDCLLFAPMGSSAEYYVLDEYGGFSEYDTVLVSGTLFQSCQTECSD
                     ATGCIIGNTISAPQAPPNYPFEAFGTLYQGLDCILFAPHGDTIAGFELEFLGDFQAGD
                     TVFVRGDLVVSCQPACTGAIACINDNTIDSIWMPVTPIAGRAVLKLANSNAAVNIAEA
                     TGSIIIDSIASQKIYLISFSDSISPDSMMIAFLGFPDVIFAEPNFEVDFPENLQLSIS
                     FPDEYAPPLDIDIAEPSSFFAQNSVETIGADSANKLSTGSGSVVAVIDNGFDMTHPFL
                     ELASFTDGYDYFSDDTDPSPDSGLGASHGTFIFGLIQLISPNCQIIPLRALGEGGKGN
                     GFAIAKSIYHALENNADVINMSFGTYKDSYLLRTACQAAHNAEILLVAAGGNDSTDVP
                     IYPAAYEGVVAVSSVDSADFLADFSNYGLSMDICAPGVDIYSALAGEYNWGTWTGSSF
                     SAPLVTAACALIRSIEPDMTADEVELHLRLSAERDLGPGSINPPDPYYAYGRLDIANA
                     VWTLNSQIPVDGCG"
BASE COUNT          785 a          755 c          832 g          900 t
ORIGIN      
        1 tatttggggc tgatcgaatg cctcctgaag atgggcaggg tggaggagtc gttttttgag
       61 aattcaagag cgctcgcgat catcaaccag aaaattccat tcacaaaaac caggcagaac
      121 aagatatcgc aagaatatct gcacaccaga gataatctcc gcgccaggct gaaggaactc
      181 agccgtctgc cagggtctgg agaacgccgg gcagactcac cccggaccat gaagaaagcg
      241 gaacaggaac tctggcagat ggaaagaaaa attcgatcga taatatatcc ttctgaagtt
      301 aacttctctt atgcttcctt acccgagaac tattatcagg ggttcctggg agatgatgaa
      361 cttcttttga actacgtggc gattggtgag gatttgggag tgtatcgtgt gcgcagggag
      421 aagaccgagt atatgccgct cgggatttcc cgtacacaaa tgacctcatt gatcggcgaa
      481 ttgcatttcc tgatggagaa atcgatctat tcggcgtctg aatcagcctc attaatgcac
      541 atcatcgaag attaccagag aagactttat gaagtattga ttaaaccgat taagccgggg
      601 acgggtgtcg ggaaaatgat tttcatgatt gacggtttct ttgcccagat accgtactgg
      661 gcattactcg attcaaaggg tgttacgctc taccaaagat ataacttgag atttatctct
      721 aacccggttg atttaaaaga gaggaaatct cttttaccgt ataaaatcag cggttcatcg
      781 gcaattttcg ttgcggaaaa tagcgggctt ccaatggtta atgctgaagg aaagcggatc
      841 aaggagcatt tcgagaacgc tgagcttttt ggaggcgccg acgcctcctg cggcaaattg
      901 ctgaattcgt tgggtcgagc ggatggtttt gtgcatatcg ccacgcatgc ctcgcgcgcc
      961 tcagagaatc cgctgttttc aaggattctc atgagtgacg gcccgttttt tcccttcgat
     1021 ctttttggcg ctaagataaa cgcaaggctg gtaacccttt ccgggtgcca gaccgccgcg
     1081 ccgggagtct tttacggcaa ctccttcagc ctggccaagg ctttttacca ggcgggcgcg
     1141 cgctatgtgc tggcttcgct ctggccgatt tccgataaga ccggcatggt cttcatggat
     1201 gaattttatg ccgaactcag aaaacacgaa gacattttcg gcgcatacca tcaggcactg
     1261 gaaaaaacac gagaattaaa tttaaacccg gccttctgga gtccgtttgt attattggga
     1321 atttagtgta tattatatag atggagagtt aatgtcatga gaagacacat tattatactt
     1381 gcagtgataa ttatatcagc aggggcgttt atacccgggg gcgcctacgg gcaggattcc
     1441 ctcttttcct cctgtggtgt tttaatcgag ggggatcaat gtgttctctt ttcaccccag
     1501 ggcggaggat ttgaggccgc gcttcaattt tacggcgact ttcagcccga tgacagcgtg
     1561 tatgtctcgg ggattatact tttcaactgt gatacacttt gtgccggaac cgatgtctgt
     1621 attgtcaata attctattgg atcctgtgac ggcgatccct atgagccttt cgacggcccg
     1681 ggcgtattgg tacagacctc agattgtttg ctgtttgctc caatgggcag ttcggcagaa
     1741 tattatgtgc tggatgagta tggcggtttt agtgagtatg atactgtttt agtgagtggt
     1801 acattgtttc aatcctgcca gaccgaatgt tccgatgcca ccgggtgcat cattggcaac
     1861 acaatttcag ctccccaggc acctccgaac tatccctttg aagctttcgg cacgctttat
     1921 caggggctgg attgtatttt gtttgcacct cacggtgata ccatagcggg ttttgaactt
     1981 gaatttctgg gagacttcca ggccggcgat acagtattcg tgcggggtga cctggtggtc
     2041 tcgtgccagc cagcatgcac cggtgcgatc gcctgtatca atgataacac aatagactcg
     2101 atctggatgc cggtaactcc aatagccgga agagctgtat tgaagcttgc aaattcaaat
     2161 gcagccgtaa atatcgcgga agccaccggc tcaataatta tcgacagcat agcttcacaa
     2221 aaaatatact taatcagttt ctccgattca atttcgccgg attctatgat gattgcgttt
     2281 ctgggatttc cggacgtaat atttgccgaa ccgaatttcg aggtcgattt ccccgaaaat
     2341 ttacagctga gtatatcgtt cccggatgaa tatgctcctc cgctcgatat agatatcgcc
     2401 gaaccttcgt cattttttgc ccagaattct gttgaaacca tcggagcgga ttcggccaat
     2461 aagctcagca ccggatcggg ttctgtcgtc gccgtgatag ataatggttt cgacatgaca
     2521 catccatttc tggaactggc ctcctttacc gatggctatg actatttcag tgatgatacc
     2581 gatccaagcc ccgactctgg tctgggcgcc agccatggca catttatttt cggactgatc
     2641 cagcttatct ctcccaattg ccagatcatt cctttgaggg ccctgggaga gggtggtaag
     2701 ggtaacggtt tcgctatagc gaaatcaatt tatcatgctc tcgaaaataa cgcggatgta
     2761 atcaatatga gcttcgggac ttataaagat agttatttgt tgcgcacagc ctgccaggcg
     2821 gctcataacg ccgagattct tctggtggct gccggaggta acgacagcac cgatgttccg
     2881 atttatcccg ctgcctatga aggcgttgta gcggtatcct cggttgattc cgctgatttt
     2941 ctcgccgatt tttccaatta tggactgagc atggacatct gcgctcccgg cgtagatatt
     3001 tacagtgcac ttgccgggga atacaactgg ggtacatgga ccggatcatc gttctccgct
     3061 ccgctggtta ccgcagcctg cgcattgata cgttcgattg aaccggatat gaccgctgat
     3121 gaggtagagc tgcatctaag actatcggcg gaaagagatc ttgggccggg ttccatcaat
     3181 ccaccggatc cctattatgc ttatggcagg ttggatattg cgaatgctgt ctggaccttg
     3241 aattcgcaaa ttcccgtgga tggctgcggg ga
//
LOCUS       DSEB01000146            2718 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1005114, whole genome shotgun sequence.
ACCESSION   DSEB01000146 DSEB01000000
VERSION     DSEB01000146.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1005114"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            634..2466
                     /locus_tag="ENO07_02705"
     CDS             634..2466
                     /locus_tag="ENO07_02705"
                     /inference="COORDINATES: protein
                     motif:HMM:NF033709.1,HMM:PF13432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HDS00907.1"
                     /translation="MIFRNRKAFVLIVVLLLLSTQADGADPSGRESLFILGAGARPLG
                     MGGAFTAVADDGSAVYYNPAGLGYLEYRELTLFHTPLYEGTNYMFGALSYPILGFGAV
                     GIGGLRLGTDDVVFRDRLGRLGSFDYSNGQYWLSYGIKVFDYIALGMNMKYLDQSLGD
                     LGTSTGSVDLGLLAHYRNTVFFGINAQDLVSGEMKLGSRGEKLPYNIKAGVAARYSTP
                     NLDFLLAGDIDKTEDVGAKFHIGGEVGIKSIFFLRGGYDRSEITFGAGVHYKFARFDY
                     AYKGHSELDPTHRLGVSFFFGPTISEQKARRVERQREEEALRAEEVRRERMADLRTRA
                     DDFYQNQVWDSAVVYYNQVLAYDTENIHAITRIHEISDKLESRARQELDTRAGELAFE
                     RLMRQYNRIADSLYESGEYEKARLEYEKVLQLDSANSKALDRLSEIEVHFDRAADSLK
                     ARGQALIAQNEYAEAVVVLTEAQELSPDDAEIGRRIAFARTRLLIDRKLRNAAEKLDE
                     GDTAAARVIFQEILELDPEEPLTLDYLEQLERETEIEPVSIGQLKADSQYWNLYLEGL
                     ELFGDGKYREAIEKWEIVLEKYPGSRDVRENIRQARLRLRNQGQ"
     gene            2477..>2718
                     /locus_tag="ENO07_02710"
     CDS             2477..>2718
                     /locus_tag="ENO07_02710"
                     /inference="COORDINATES: protein motif:HMM:PF13581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDS00908.1"
                     /translation="MGLFTKAVKESQFSFDAKEEKVGEIKDYVRETLEGTPFSEKELT
                     GILLAIEEACTNVIRHAYLYAPGGTIRLKITLTSDKA"
BASE COUNT          696 a          612 c          771 g          639 t
ORIGIN      
        1 cgccagggac gcgcgtttga acttgataat ttgttgcttt ccggtactat ttcatatgag
       61 caactggaat atccgggtac tatcagctcc aatactctca gcttcaattt cggggatggt
      121 atcatatttg atgccgagcg ttcggcggtt ctgaatctgg aaattgtaat tgatgatcag
      181 gccgcacttg aaaatttcat aatatcactg gattcagatc atataactgc aaatgattac
      241 aggttcgaag tactcggcag cccgttggtc gtaaagtcta ttgatggcgg cgcttttgcc
      301 atttccaaaa gctatggcac ggtacaatcg gaatttgaaa attcatttta taattatccc
      361 aatcccttta accctgatgt tgaggcaact tcgatagttt attatctgcc gagtgccagt
      421 gatgtagatt taacaattta caccttgatc ggcgaggaag tgtttaccac aagtatccct
      481 gcgggttccc ccggagcgca gggaggcacg atcaacagca tctactggga tgggcgtaac
      541 gatgaggcgc ttgaagttcg cgaaggggta tatctggcag tcgtaaaata ttcgggcggt
      601 gaagcctgga caaaaattgc ggtggtgaag tagatgatat tcaggaacag gaaagcattt
      661 gtcttgatag tcgtgctgct gcttctgagc acgcaggccg atggcgccga tccctcgggc
      721 cgtgagagcc tgttcatact gggcgccggc gccaggcctc tgggtatggg gggagccttt
      781 acagcagttg ccgatgacgg ttcggcggtc tattataatc cggccgggct tggatatctg
      841 gagtaccgcg agctgacgct gttccatacc ccgctttacg agggaaccaa ttacatgttc
      901 ggggctttgt cctatccaat tctcggattc ggcgcggttg gaataggcgg cctgagactt
      961 ggcaccgatg atgtcgtgtt tcgcgacagg ctgggcaggc tgggcagttt cgattacagc
     1021 aacggccagt actggctgtc ttacggcatt aaggtcttcg actatatcgc gctcggtatg
     1081 aatatgaaat atctggacca gtccctgggt gacctgggca cttccaccgg ctctgtcgat
     1141 ctcgggctcc tggcacatta tagaaacaca gtgttttttg gcattaacgc gcaggatctt
     1201 gtgtccggcg aaatgaagct cggcagcagg ggagaaaagc tgccatacaa tatcaaggcg
     1261 ggtgtggccg caagatacag cacccccaat ttggatttcc tgcttgcggg tgatattgac
     1321 aagactgagg atgtcggtgc aaaattccat atcgggggag aggtcggcat caagagtatt
     1381 ttcttcctgc gagggggata cgatcgttcc gagattacct tcggcgccgg ggttcactac
     1441 aaatttgcca ggttcgatta tgcctacaag ggacattccg aactggatcc cacccatcgg
     1501 ctgggcgttt cgttcttctt tgggccgaca atctcggagc aaaaagccag gcgggtagaa
     1561 cggcagcgcg aggaagaagc gctgcgggcc gaggaagttc gcagagaacg catggccgac
     1621 ctgagaacca gggctgatga tttctatcag aaccaagtct gggattccgc ggttgtatat
     1681 tacaatcagg tgcttgccta tgatactgaa aatattcatg cgataacgag gattcatgaa
     1741 atcagcgaca aacttgagag tcgtgccagg caggaattag acacccgggc gggtgaactt
     1801 gccttcgaga gattgatgcg gcaatataat agaatagcag attcgctcta tgaatcaggt
     1861 gaatatgaaa aggcccggct ggaatatgaa aaagtactcc agcttgattc agccaattca
     1921 aaggccctgg accgcctatc tgaaatagag gttcattttg accgggcggc ggattcactg
     1981 aaagcgcgtg gacaggcttt gatcgctcag aacgagtatg ccgaggcggt ggtggtattg
     2041 accgaggccc aggagttaag cccggacgat gctgaaatcg gaaggcgtat agcatttgcc
     2101 aggacccggc tcctgattga ccggaagctc aggaatgccg cagagaaact tgatgaaggt
     2161 gatactgctg cggcccgcgt aatcttccag gaaatactcg aactcgatcc tgaagagccg
     2221 ttaacgctcg attatcttga acagctcgag agagaaactg aaatcgagcc ggtatcgatc
     2281 gggcagttaa aagccgacag ccagtactgg aatctctatc tggagggcct ggagctgttc
     2341 ggggatggaa agtacaggga ggcgattgaa aaatgggaga tcgtgctgga aaaatatccg
     2401 ggctcccggg atgtaagaga aaatatacgg caggcgcggc tgcgcctgag gaatcagggc
     2461 cagtaattca gaaaatatgg gtctattcac aaaagctgta aaagaatcgc agttttcctt
     2521 cgacgccaaa gaggaaaaag ttggcgagat taaagattat gtcagggaaa ccctggaggg
     2581 cacccccttc tctgaaaagg agctcaccgg aatactgctg gcgatagaag aagcctgcac
     2641 caacgtaatc aggcatgcct atctgtatgc tccgggcgga acgatccgcc tgaaaataac
     2701 actgacctcg gataaagc
//
LOCUS       DSEB01000147            5241 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001179, whole genome shotgun sequence.
ACCESSION   DSEB01000147 DSEB01000000
VERSION     DSEB01000147.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001179"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1352
                     /gene="guaB"
                     /locus_tag="ENO07_02715"
     CDS             <1..1352
                     /gene="guaB"
                     /locus_tag="ENO07_02715"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDS00909.1"
                     /translation="IMLNIPVVASAMDTVCEAELAIALARQGGLGFIHKNMSAEQQAA
                     EVDKVKRSESGMIVNPITLPPDEPIGKALEFMERFSISGIPITEDNKLVGIITNRDLR
                     FHRNLNLKISDVMTRDNLITVPPETDMERAKDILHENRIEKLPIVDEEGYLKGMITVK
                     DIMKKIMYPDACKDDKGRLRVGAAVGVSGDMLERARLLVDSEVDVLVIDSSHGHHEGV
                     LNAVSQIKKNFPDVALVAGNVAMESGAEALIKAGAECVKVGIGPGSICTTRVVTGAGV
                     PQATAIMNCARACRRHDIPLIADGGVKYSGDITKAIACGADCVMIGSLFAGTKESPGE
                     IVLYDGRSYKVYRGMGSIEAMRQGSGDRYFQHNEDIKKLVPEGIEGRVPYKGELAETV
                     YQLIGGLRAGMGVCGTKTIADLKRDGKFVQITNASLFESHPHDVQITKESPNYRTRM"
     gene            complement(1538..2254)
                     /locus_tag="ENO07_02720"
     CDS             complement(1538..2254)
                     /locus_tag="ENO07_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00910.1"
                     /translation="MKTLVHYVLGLLLVTLIIYFLPGCGGDRSTDSNGATIEPLTVTD
                     IDGNVYQTVKIGDQLWMAENLRVTHYRNGQLILCIPDSALWAWTTLPLAAYCNYNNDE
                     SNVSVYGRLYNWYAVTDTSKIAPEGWHVPSDAEWQTLVDYLGGPEIAGGKMKEPGIDY
                     WITPNTGATNESGFSALPGGYRDPNGHFDYIGIFGHFLSSSASGYTYAWKWRLNYYDS
                     AIERLSCNKRSGFSVRCVKD"
     gene            complement(2315..3262)
                     /locus_tag="ENO07_02725"
     CDS             complement(2315..3262)
                     /locus_tag="ENO07_02725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00911.1"
                     /translation="MHKKRCLLSLMIFFAFTGLVMTNTVAAYVCGDSNGDQNVNVSDA
                     VNIINYVFVGGEPPVPMEAGDCNCDGNCNISDAVAIINFVFIGGYEPCDPNGDGIPDC
                     GFYCPPTVTDYDGNIYQTVQIGDQCWMAENLKVTHYRNGNSIPSVTDYSAWSSLTTGA
                     YCNYGNDISNVAVYGRLYNWHAVNDSRGLAPQGWHIPSDTEWKQLEMYLGMSQADVDD
                     YGWRGTNQGGQLKETGTSHWLAPNTGATNISGFTALPAGDRLDTGAFYYIQQYTGFWS
                     TSLAGSNWAWNRGLAYNVSSIDRTGYLKTFGYSVRCVKD"
     gene            3772..>5241
                     /locus_tag="ENO07_02730"
     CDS             3772..>5241
                     /locus_tag="ENO07_02730"
                     /inference="COORDINATES: protein motif:HMM:PF00009.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDS00912.1"
                     /translation="MKEYKIEQLRNIGLVGHGTSGKTSFAEAILNIGGITSRLGKVEE
                     GNTVSDYTEEEINRKISISATALNIEYKNTKFNLIDMPGYADFIGEVVGGLRVADVAA
                     ILFDATGGIEVGTEMAFRYTDKYNTPRCFIINKIEKEHAEFGSKVQILQDHFGIHVIP
                     VQLPIGEGLNFKGIVDLIKMKAYSYDESGTAAAMDIPDDMQSSAQEAHEKLIENVAES
                     DDALLEKFFDAGELSPEEIQAGLKKGISEQSIYPVFAVSSLTMAGVTTFLDFAAEYFP
                     SPADFGEVKGLKPDSDEEISRPVSEGAPPVIFIFKTVSESHVGELAYFRVFTGSVKQG
                     DELYNTSFEDTEKIAQMYNVNGKNRKEVSKVGPGDMGAFVKLRKTHTGDTLTSKSDPL
                     MIPKLEFPEPVVDIGVRPISKGDEEKISGGLAKLHEEDPTFMMRVDPELKQTLLYAQG
                     ELQMDIILNKLKKRFGVDVETEKPRIPYRETIKGKAEVQY"
BASE COUNT         1369 a         1229 c         1365 g         1278 t
ORIGIN      
        1 atatcatgtt gaatataccg gtggtagcct cggctatgga tacggtctgc gaggcggaac
       61 tggctattgc gctggcgcgg cagggcggcc tgggtttcat ccacaaaaat atgtccgccg
      121 agcagcaggc ggcagaggtg gacaaagtca aacgttccga gtcgggcatg atcgtcaatc
      181 cgattaccct gccccctgat gaaccgatcg gtaaggcgct ggaattcatg gagcgctttt
      241 ccatatcggg cataccgatc actgaagaca acaagctggt ggggatcata accaaccgtg
      301 acctgaggtt ccatcgcaat ctgaatttga agatctccga tgtgatgacc agggacaacc
      361 tgatcactgt gccgcccgaa accgatatgg agcgggccaa ggatatactg catgagaaca
      421 ggatcgagaa gcttcccatt gtcgatgagg aaggttacct gaagggcatg atcacggtca
      481 aggatattat gaagaagatc atgtatccgg atgcctgcaa ggatgacaag ggccgtctgc
      541 gcgtgggagc ggctgtcggc gtttcaggcg atatgctgga gcgtgcccgg ttattggtcg
      601 attctgaggt ggatgtgctg gtaatagact cctcgcacgg tcatcatgag ggagtcctga
      661 atgctgtttc gcaaatcaaa aagaatttcc cggatgtcgc gctggtagcc ggcaatgtgg
      721 ccatggagtc gggagcggaa gcattgatca aggccggcgc tgaatgtgtg aaggtcggga
      781 tcggccccgg ttcgatctgc accacccgtg tggtgacagg cgccggtgtg ccccaggcga
      841 ctgcgattat gaactgtgcc agagcctgcc ggagacatga tattccattg atagccgacg
      901 gcggtgtaaa gtactccggc gatattacca aggcgattgc ctgcggcgcc gactgcgtga
      961 tgatcgggtc gctgtttgcg ggcaccaagg agtcgccggg cgagatcgtt ctgtatgacg
     1021 gccgttccta taaagtttac cgcgggatgg gttcgatcga ggccatgcgc cagggttcgg
     1081 gcgacaggta tttccagcat aacgaggata tcaagaagct ggtgcccgag ggtatcgagg
     1141 gacgtgtgcc atataaggga gagcttgcag agacggtcta tcagttgatc ggcggcctgc
     1201 gcgcaggaat gggtgtctgc gggacgaaga cgattgccga cctcaagcgt gacggcaagt
     1261 ttgtgcagat taccaatgct tcgcttttcg agtcacatcc gcacgatgtg cagatcacca
     1321 aagagtcccc aaattaccgg acacggatgt aggatataaa ttttcgcgta gcgcaaagac
     1381 ttacgccgcg cacgaataga tttggtttat gggtggcacg ctcaaattaa tttgggcgtg
     1441 tttttatata tgggtacctg cccaaagcga gtttgggcag gccaccccta ttgtaatatc
     1501 attcctttcc ggcttcagtc taaaccaggc atgccgatta gtccttgaca cagcgtacgg
     1561 aaaatccgga gcgtttatta cagctcaggc gttcgatggc tgagtcatag tagttgagcc
     1621 gccacttcca tgcgtaggta taaccgctcg ccgaagaaga caggaagtga ccgaatatcc
     1681 caatgtagtc aaaatgacca tttggatcac ggtaacctcc gggcagcgcg gaaaaaccgc
     1741 tttcattggt ggcacctgta ttaggggtaa tccagtaatc tatcccgggt tccttcattt
     1801 tcccgccggc aatttcaggc ccccccagat aatccaccaa tgtttgccat tctgcatcac
     1861 tgggtacatg ccatccctct ggcgctattt tggatgtgtc ggtcactgca taccaattat
     1921 ataatctacc gtaaacagag acgttgcttt cgtcattatt gtaattgcag taggctgcta
     1981 acggtaatgt agtccaagcc caaagggcgc tatctggaat acaaagaatg agttgcccat
     2041 tacggtagtg agttactctc aaattctcgg ccatccacag ctgatcaccg atcttgactg
     2101 tctgatagac attgccatct atatctgtaa cagtgagagg ctctatggtt gctccattag
     2161 aatcagtgct tctgtccccg ccgcacccgg gcaagaagta tataatcagg gtcacaagaa
     2221 gaagcccgag tacataatga accaacgtct tcatgtattt acacctccgc aaaaggtttt
     2281 actagggcta atgcccgaat agagaaacaa ttttttagtc cttgacgcag cggacggaat
     2341 agccgaatgt ctttagatac ccagttcggt caatgctcga tacattatag gctaggccac
     2401 gattccacgc ccagttagaa cctgccaaac ttgtggacca gaagccggta tactgttgaa
     2461 tgtaataaaa agcacctgta tcaagcctgt caccggcagg aagagcggtg aagccactta
     2521 tattggtcgc gccagtgttg ggcgccagcc aatgggaggt tccggtctct ttcaactgcc
     2581 cgccttgatt agtgccacgc caaccatagt catctacatc ggcctggctc ataccaagat
     2641 acatttccag ctgtttccat tcagtgtcgc tcggtatgtg ccatccttga ggggctagac
     2701 ccctgctgtc atttacagcg tgccaattat acagcctacc ataaactgct acattactaa
     2761 tatcattccc gtagttgcaa tatgcacccg tggtcaggct tgaccacgcg ctataatcag
     2821 tgacactagg tatgctatta ccgttacgat agtgtgtaac tttgagattt tcagccatcc
     2881 agcattgatc accaatttgt acggtttgat agatattccc atcataatca gtaactgtcg
     2941 gcgggcaata gaaaccgcag tcagggatac cgtcgccatt cggatcacat ggttcataac
     3001 cgccaatgaa aacaaaattt atgatcgcga ctgcgtcact aatattgcag ttgccatcgc
     3061 agttgcagtc gcctgcctcc atcggaacgg ggggctctcc cccaacaaag acgtagttaa
     3121 taatattaac cgcatcactt acattaacat tctgatcgcc gttagagtca ccgcagacat
     3181 aagctgcgac cgtattcgtc atgactaaac ccgtgaatgc aaagaatatc ataagagaaa
     3241 gcaaacatct ttttttgtgc atatggatgc cctgccataa ttaataattg cgaaccaaat
     3301 ttcccaaaag ggtgaatccc cccaggacgt tagagttata tatttaaaga gacgctaaaa
     3361 ttggaaagat gcgctttact ttccagaatt ataaatatgc tgcctaataa ttgatgcctt
     3421 tctgccagaa atttattttt gcaacataga gaagtcaagc aatattttat attggaaaaa
     3481 aaattgcgat acttttttta aggtggaact cagggcacct ggcagaatca ctccactcat
     3541 caaagccgga tctttgatca cgtttcggat tcgtccctac tcgttcatgc gggcaggcta
     3601 cctggttcaa tgtttcgctc cctcagaatg actgcaatca taaatatttt tcccgaaagc
     3661 atcgtaaaaa atggcagttt taattgcgaa aacatctatt gacaagtttg gggtgattta
     3721 ttaaattccc gcacaatttt cggtggaatt gaaaaaccag gagttagtct tgtgaaagaa
     3781 tacaaaattg aacagctgcg aaatatagga ctggtcggac atggtacctc cggtaagaca
     3841 tcgttcgctg aggctattct caatatcggc ggcataacca gccgtctggg aaaagttgag
     3901 gaaggtaata cggtttcgga ttatacggaa gaggagatta accgcaaaat ttcaatttca
     3961 gccaccgcct tgaatattga atataagaac actaagttca atctaatcga tatgcccggc
     4021 tatgccgatt ttatcggcga agttgtgggc ggactgcggg ttgcggatgt ggccgcgatc
     4081 ctttttgatg ctaccggcgg aattgaggtg ggcaccgaga tggccttccg ttatactgac
     4141 aaatataaca cgccgcgctg ttttattata aataagattg aaaaagagca tgccgaattc
     4201 ggatctaaag tgcagatctt gcaggaccat ttcggcatcc atgtcatccc ggtccagctt
     4261 ccgatcggcg agggcctgaa ttttaagggt atagtcgacc tgatcaaaat gaaagcctac
     4321 agttatgacg agagcggtac ggccgcggct atggatattc ccgacgatat gcagtcgtcc
     4381 gcccaggagg cgcatgagaa gctgatcgaa aatgtagccg aatcggatga tgcgctgctg
     4441 gagaagttct tcgatgccgg tgagctttcg cccgaggaga ttcaggccgg attgaagaag
     4501 ggtataagcg agcagagcat atatcctgtt tttgcagtgt cgtcactgac aatggccgga
     4561 gttacgacat ttctggattt tgccgcggaa tattttccct cacccgcaga ttttggcgag
     4621 gtcaagggat tgaagccgga ttcagacgag gaaatcagcc gcccggtttc cgagggggcg
     4681 cccccggtga tctttatttt caagaccgtt tccgagtcgc atgtggggga gctggcctat
     4741 ttccgggtat ttaccggatc ggtcaaacag ggtgacgagc tttataatac ctcattcgag
     4801 gataccgaaa aaatcgctca gatgtataat gtcaacggca agaaccgcaa ggaagtgagc
     4861 aaagtggggc cgggggatat gggagctttc gtgaaactgc gcaaaaccca taccggggac
     4921 acattaacat ccaagagcga tcccctgatg atacccaaac tcgaattccc ggagccggtt
     4981 gtggatattg gcgtcaggcc gatcagcaag ggtgatgagg agaaaatctc gggcggcctg
     5041 gccaagctgc atgaggagga tccgaccttc atgatgcggg tcgatccgga attgaaacag
     5101 acgctgctgt atgctcaagg cgagctgcag atggatatta ttttgaacaa gctcaaaaag
     5161 cgtttcgggg tcgatgtcga gaccgagaag cccagaatac cttatcgtga gacgatcaag
     5221 ggcaaggccg aggtccagta c
//
LOCUS       DSEB01000148            5249 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001178, whole genome shotgun sequence.
ACCESSION   DSEB01000148 DSEB01000000
VERSION     DSEB01000148.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001178"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..455
                     /locus_tag="ENO07_02735"
     CDS             <1..455
                     /locus_tag="ENO07_02735"
                     /inference="COORDINATES: protein motif:HMM:PF02873.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HDS00913.1"
                     /translation="QGPRWEGNKYFAFSYRNSILKKTHEIVLRARFRYVPEKPAVLLK
                     KQDEILELRNQRHPRTDCSAGCFFKNIEKADEPYGKLAAGFLLEKVGAKRMHIGGAGV
                     FDKHANILINASGKARAEDVRKLAEALKKKVKDEYGYNLEEEITFLGD"
     gene            518..>5249
                     /gene="sprA"
                     /locus_tag="ENO07_02740"
     CDS             518..>5249
                     /gene="sprA"
                     /locus_tag="ENO07_02740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HDS00914.1"
                     /translation="MNLRNRFHFISLIGVLVILLGASPGLIAQDNGESEEESPEAGFS
                     RLNIPLDYETPYITPFPQKPLDPGISFAGEAPGLLNNRRTQYNVVAGWDKKTRNLYYS
                     RTINEHKVETDRYADYLENARVYGSAEVANYWRQNLYQSTKKAERDKGAGLLNVKVDL
                     GDQVGGVIKAIAGEGTAGLDVTGYQKISFSGRSQWTDKESDALNRINKFPSLDMEQVS
                     RYSIKGTIGTKISVEVDQNSERKTDLANTIRIRYKGSEDEIIQSIEAGNTNLSLANTQ
                     FVGYSQRVQGLFGIKATAKIGALDLTAIVSQEKGSTESSSFEAGAAKSTNYKRDYDYL
                     ERTFFWLFFPEDDIDTIIESEIYVYHNIQSGANTEINEQWNYANVAVDPENFGQYPQE
                     DRTTTVKRLDYDDYYVYNKSLYLEMFTYDRTRMIAGWFRVRMNNGEIVEIGNRLGDTL
                     SLKLIALEQNVADSTYQTWRNEWRNVYSLGARDIDAEGIDVQVYLGAPGTETQADKNL
                     PHQNGTPYIQIMGLDQRDVTGADNPDGIIDSKNSNILLRDRGYLIFPEPNPFASTVSY
                     APDGETLNVTVPEIYNTRRSTITGNPSQYTEYYIQFETSQRQTEYYLGRVNIIENSER
                     VILNGEELKKDRDYKIYYEIGRITFLTDAVSDPNADLQIDFEYEPFITSEKKNLFGVR
                     GEYEFNPNFKIGSTVLYKGEKSTDRKPRVGQETSKSLVWDADFAYSTDLPFLTSLTDA
                     LPLVEATADSRIAISGEVAQSRPNPNTAGEAFVDDFESAKETFSLGITRYQWTKSSAP
                     DLVDRELRGRLIWFNPYDPYSVTDIYNRDVRGNDRKTTMSILFDPLEFVPDPGSEDEC
                     NPDSIAVTPAESWAGIMRAYSTGSQDQTRAQLLEVRMKINNGRIPSDGKIYIDLGDIS
                     EDINGNGTLDTEDKNGNGILEQFEDVGLDGLADTAEACYDSVTNPDPAGDNYSYENKN
                     DYSQINGTEGNRFDASGARPDTEDLDGKGSLNTVEKFFRLVIDLADNKYRVDGSNLNN
                     WITYQIPLRDSLNYEAINNPDWGRIAYSRIWLTGFDSTVVVEIADINLVTTRWEEDSI
                     RPLNIDRTIATDFPEFNVSAINTEENLDYTPPPGVSGYYDRTTETYEKEQSLLLRFSN
                     LQFYPVDTQITVDSTVIDTFVMVPDTAIAARELFRSEDYAGYKKIRMYVHGPDSLPEG
                     PSPIHFIFRLGNNAENYYQFQTNIYPGWSDSNEVVMDFSEATLVKDSLIRLQAEDPGI
                     RELKVGKYAVRGSPSLTSVLYFGLAVTNTDTVNYEPISGEIWLDELRVSDVRNDPGTA
                     MRLSVSGGLSDFVTYTFNYRNQDEYFRNLTASNSSNLGSGNSETNYSYSAKVNIHKIL
                     PPSWGASLPFTYSVSERTSVPRLKPGSDIIVPPEQEEEQTSRSTNTRFSISQSFRKQG
                     GNLLFGLLLNRLNTSYSYSESDSKSPTVPFSHSESYQARATYDMTPKASLNIPILFWL
                     RPIPLIPQSLKDTQLGLLPSRLNLSGTVKKNYSKSINNRNYKSETYNRTLDGTLDLSW
                     NIFKSLSADYNLRTTRDIRHTDDIN"
BASE COUNT         1460 a         1272 c         1323 g         1194 t
ORIGIN      
        1 gccagggacc gcgctgggaa ggcaataaat actttgcctt ttcatacaga aatagtatat
       61 tgaaaaaaac ccatgaaata gtgctgaggg cgcgttttag atatgtgcca gagaagcctg
      121 cggttttatt gaaaaaacag gacgagattc ttgaattgcg caaccagaga catccccgga
      181 cggattgctc ggcaggttgc ttttttaaaa atatcgaaaa agcggacgag ccgtacggaa
      241 aactggcggc tggattttta ctcgaaaaag taggagcaaa acgaatgcac attggcggtg
      301 ccggagtatt cgacaagcac gcgaatattt tgataaatgc ttccggaaag gcccgagccg
      361 aggatgtacg caaactggcg gaggcgttga agaagaaagt aaaagatgaa tacggatata
      421 atctggaaga agaaataact tttttgggcg attgataaaa aggagtgtct tatagcgtat
      481 taatacaggt taaggaattt aaatataagg tctgtttgtg aatttaagga atagattcca
      541 ttttattagc ctgatcggcg ttcttgttat tctgctgggt gcaagtccgg gactgattgc
      601 gcaggataat ggagaatccg aagaagaatc gcccgaagcg ggtttcagcc ggcttaatat
      661 cccactggac tacgagaccc cgtatatcac ccccttcccc caaaaacccc tggatccggg
      721 aatatcattt gccggagaag cgccggggct cctgaataac aggagaaccc agtataatgt
      781 cgttgccgga tgggataaaa agaccagaaa tttatactat tcgcgcacca tcaacgagca
      841 taaggtcgag accgatcgat acgctgatta ccttgagaac gccagggttt acggaagtgc
      901 cgaagtagcc aattattggc gtcaaaatct gtaccagagc acaaagaagg ccgagagaga
      961 caagggcgcg gggcttttga atgtcaaggt ggatcttggt gatcaggtag gcggggtcat
     1021 caaggcgatc gcgggcgaag gaactgccgg actggatgtt accggatacc agaagatatc
     1081 tttctccgga cgaagccaat ggactgataa ggaatcggat gccttgaacc ggatcaataa
     1141 atttccttct ctggatatgg agcaggtttc acgttacagc atcaagggca cgatcggaac
     1201 caaaatatcg gttgaggtgg atcaaaactc ggaaagaaaa acagaccttg ccaataccat
     1261 caggatcaga tacaaaggct ccgaagacga aattattcag tcgatagaag ccgggaatac
     1321 gaatctgtcg cttgccaata cccagtttgt aggttattcc cagagggtac aggggctgtt
     1381 cggcataaag gccacggcca aaatcggagc gcttgatcta acggcaatcg tctcgcagga
     1441 aaagggttcg accgaaagtt cgagcttcga ggcaggagcc gccaaatcaa ccaactataa
     1501 gcgcgactac gattatctgg agcggacatt cttctggctc ttcttcccgg aagacgacat
     1561 tgacacaatc attgaatcag aaatatatgt atatcacaat atccagagcg gcgccaacac
     1621 cgaaataaac gagcaatgga attacgccaa tgtggccgtt gatccggaaa acttcggcca
     1681 gtatccacag gaggaccgaa ccaccacggt aaagcgtctc gattacgacg attactatgt
     1741 ttacaacaag tcgctgtatc tggaaatgtt tacctatgat cgcacgcgta tgatcgccgg
     1801 atggttccgt gtccgcatga acaacgggga aatagtcgaa atcggcaatc gccttggaga
     1861 tacgctatcg ttgaaattaa tcgccctgga acagaatgtg gctgacagca cttaccagac
     1921 ctggcgcaat gagtggcgca atgtttacag cctgggcgcc cgcgatattg acgcggaagg
     1981 gatcgatgtc caggtatatc tcggggctcc cggaacggag acgcaggcgg acaaaaacct
     2041 gcctcatcaa aatggcacac cctacatcca gatcatggga ctggaccaga gggatgtaac
     2101 cggggcggat aatcccgatg gaataatcga ctccaaaaac agcaatatat tgctgaggga
     2161 tcggggctat ctcattttcc cggaacccaa tccctttgcc tccacagtat cttatgctcc
     2221 cgacggtgag acgttgaacg taactgtgcc cgaaatatat aataccaggc gcagtacgat
     2281 aaccggcaat ccctcacaat ataccgaata ctatatccag ttcgagacct ctcaacgcca
     2341 gaccgaatat tacctggggc gggtaaatat tattgaaaac tccgagaggg tgatcctcaa
     2401 cggtgaggag ctgaaaaaag acagagatta caaaatttac tatgaaatcg ggagaataac
     2461 atttttaacc gatgctgtct ccgatccgaa tgccgatctg cagattgact tcgaatacga
     2521 gccctttatc acatcggaaa agaagaacct tttcggagtg cgcggcgaat atgagtttaa
     2581 tcccaatttc aagatcgggt cgacggtgct ttacaaaggg gagaaatcga ccgaccgcaa
     2641 accgcgtgtc ggccaggaga cctcgaaatc gctggtctgg gatgccgact tcgcatattc
     2701 gaccgacctg ccgttcctga catcgcttac cgatgctctg ccgctggttg aggccacggc
     2761 tgattctcgt atcgccattt cgggtgaggt cgcgcaatcc cggccaaatc cgaacaccgc
     2821 gggcgaggct ttcgttgatg actttgaaag cgccaaggag accttctcgc tgggaattac
     2881 acgttaccag tggaccaaat catccgcgcc ggatctggtt gatcgcgagc tcaggggacg
     2941 actgatctgg ttcaatcctt acgaccccta ttcagtgacc gacatttata atcgcgatgt
     3001 gcgcgggaat gatagaaaga ctacaatgag cattcttttt gatccgctgg aatttgtccc
     3061 cgatcctggt tctgaggatg aatgtaatcc ggattcgatc gcagtcaccc cagccgaaag
     3121 ctgggccgga attatgcgcg cttattccac cggcagccag gatcagacac gtgcgcaatt
     3181 gctcgaagta aggatgaaga tcaataatgg gcggattccg tccgatggga agatatacat
     3241 cgacctgggc gacatttctg aagatataaa tggaaacggg acccttgata cagaggataa
     3301 aaacgggaat ggcattcttg agcagttcga ggatgttggc cttgacggtc tggccgacac
     3361 ggctgaggcc tgctatgatt cagttacaaa tcctgatcca gcaggcgaca actattccta
     3421 tgaaaacaaa aacgactatt cacagataaa cggcaccgag ggaaataggt ttgatgccag
     3481 cggcgctcgt ccggatacgg aggatcttga cggcaagggt tccctgaaca cggtagaaaa
     3541 attcttccgg cttgtgattg atctggcgga caataaatat cgcgtggatg gatcgaatct
     3601 caataactgg ataacctatc agattcctct gcgggattca ttgaactatg aggccatcaa
     3661 taatcccgac tggggcagga tcgcttacag ccgaatctgg ctgaccggct tcgattccac
     3721 cgtcgtggtt gaaatagccg atattaatct ggtgaccacg cgctgggaag aggactcgat
     3781 tcgtcccctg aacatcgatc gaaccattgc aacggatttt ccggaattca atgtgtcggc
     3841 tatcaatact gaagagaatc ttgattatac ccctcccccg ggtgtgagcg gatattatga
     3901 cagaaccacg gaaacctacg agaaagagca gtcgctgctg ctgcgtttca gcaacctgca
     3961 attctaccct gtcgacacgc agatcactgt cgattctacg gtaatcgata cctttgtcat
     4021 ggtgcccgac accgccatag ccgcacgcga gcttttccgt tccgaggatt atgcgggcta
     4081 taaaaagatt agaatgtatg tacatggccc ggattctctt cccgagggac catcacctat
     4141 ccattttatc ttccgcctgg gcaacaatgc cgagaattat tatcaatttc agaccaatat
     4201 ctaccccggc tggagtgatt ccaatgaagt tgtgatggat ttcagcgaag ccacgctggt
     4261 caaggactcg cttatcagat tgcaggcgga ggatcccggt atcagggaat tgaaggtcgg
     4321 caaatacgct gtcaggggaa gtccatcctt gacttcagtc ttgtatttcg ggttggcggt
     4381 aaccaacacc gatacggtca attatgagcc catatcgggg gaaatctggc tcgatgaact
     4441 gcgggtttcg gatgtgcgca atgatccggg aaccgccatg cggctctccg tttccggcgg
     4501 gctctctgat ttcgtcacct acactttcaa ttaccggaac caagatgaat atttccggaa
     4561 tctgaccgcc tcgaacagct ccaatctcgg ctccggcaat tccgaaacca actacagtta
     4621 ttcggccaag gtcaatatcc acaagatcct tccgcccagc tggggagcct cgctgccttt
     4681 tacctatagc gtgagtgaaa ggacctccgt tccaaggctg aagccgggat cggatattat
     4741 cgttcccccc gagcaggagg aggaacagac aagccgatca accaatacac gtttttcgat
     4801 atcgcagtca ttcaggaagc agggcggcaa tcttctcttc gggctcctgt tgaaccggct
     4861 taatacttca tattcataca gtgagtctga cagcaagtct cctactgtac cattctcgca
     4921 ctcagaatcg tatcaggcca gagcaactta cgatatgact cccaaggcct ccctgaatat
     4981 cccgatcctg ttctggctcc ggcctatacc tctcattcca cagtcgctca aggatactca
     5041 gcttggtctt ctaccgtcca gactcaacct ctccggaacg gtcaagaaga actattcgaa
     5101 gagcatcaat aaccggaatt acaaatcgga aacatataat cggacgcttg acggcactct
     5161 ggacctgagc tggaacatct tcaagtcatt gagcgcggat tacaatttgc gcacgacccg
     5221 ggatatcagg catacggatg acataaata
//
LOCUS       DSEB01000149            3273 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003393, whole genome shotgun sequence.
ACCESSION   DSEB01000149 DSEB01000000
VERSION     DSEB01000149.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003393"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(276..887)
                     /locus_tag="ENO07_02745"
     CDS             complement(276..887)
                     /locus_tag="ENO07_02745"
                     /inference="COORDINATES: protein motif:HMM:PF09186.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDS00915.1"
                     /translation="MEDSYKTIKGPARTEIKIQRSKFIASAFPCQSQEEAEAEIEKIS
                     SEFHDANHNCYAYRIYPFRGLDIFRYSDAGEPSGKAGRPIHDSIVSAELFNTGIVVTR
                     YFGGIKLGTGGLKRAYRDAARNVLESARIIEVLLTQKYRVKFPLNLTNVILRTLSAEG
                     IKIVDSNYTDEAEIIFVVRLSIMDQLEEALISATNARIELTRI"
     gene            complement(908..1138)
                     /locus_tag="ENO07_02750"
     CDS             complement(908..1138)
                     /locus_tag="ENO07_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00916.1"
                     /translation="MTMTEEEKIENLRKLVDRTAEDIQSGDLTEEKARELIAKTREEA
                     EDLIPDDMEKYDMIYGARFERLIDQFIKAKQE"
     gene            complement(1148..2062)
                     /gene="dprA"
                     /locus_tag="ENO07_02755"
     CDS             complement(1148..2062)
                     /gene="dprA"
                     /locus_tag="ENO07_02755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDS00917.1"
                     /translation="MPGLPCIEIGIFFMSEYNKELLIALREKAKLGSKTLQMLLSHFQ
                     SLENIVSADIEELTEFPRISEKKAAVIHEIERHLDGIKQDLELYRREGIETLTIFDDN
                     YPELLRQINSPPFVLYWKGNFPVEDEQTIAVVGTTQASAEGIEIAVDFSNALAEKGAV
                     IVSGLARGIDTASHIGAIKAEGRTYAVLGCGFNHIYPPENKKLSEEILEKGALISEYS
                     PDTRVTTGRLISRNRIIVGLAQSVVVGELDKKSRGTINAAERARELGKLLFAIGRKDL
                     PVDQGLIDYGAIPLEDISKAELISDHSL"
     gene            complement(2159..2695)
                     /gene="amrA"
                     /locus_tag="ENO07_02760"
     CDS             complement(2159..2695)
                     /gene="amrA"
                     /locus_tag="ENO07_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HDS00918.1"
                     /translation="MHLTQEEKDHLKELALESIRTKFQNEPEPELGIESETLKENCGA
                     FVTLKIRGSLRGCIGLVVGMKPLYKTITEMARAAAFDDPRFTPLAEKEVDKLEIEISV
                     LSPLEKIDDPNQIEVGKHGIMMRRGYHSGLLLPQVATEYGWDRKTFLRHTCLKAGLPE
                     NAYQDRATEIQIFTAEIF"
     gene            complement(2704..>3273)
                     /gene="amrB"
                     /locus_tag="ENO07_02765"
     CDS             complement(2704..>3273)
                     /gene="amrB"
                     /locus_tag="ENO07_02765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HDS00919.1"
                     /translation="GSAYLTPLGKLFIDQELGSKISQISPSKVYLSNKGHTGGGTRQE
                     HSLEVQLPFLQMMLGDFKLVPIIMAEQEWDIITALGETLAAALKGTNTLIVASTDLSH
                     FYTAEIASQKDAIIRAKVEAFDPQGLYKSIAKGEAEACGGGPMAAAMLACRKLGANKI
                     TVTGQSDSGRVTGDSREVVGYLGAVIYSD"
BASE COUNT          742 a          860 c          768 g          903 t
ORIGIN      
        1 cctgtaacct ctcttcagga gcacccccac atgggcccgg gaaacagaca gaaatgtcgc
       61 ctccattagc gaacacagaa atgacaccag cagcgcaagc agaaaatatg agattagagc
      121 agtcatcggg gtatataaat cctgccaaaa taaatatctg aagaacgggg gctgcgacta
      181 aaactttcgc cggggatttc catttgatta tagtttaagg aatacgttta atattgtcaa
      241 agcaaataat tgctgccggc acgattcggc cggcatcaga ttcttgtcag ctcgattctg
      301 gcgttggttg cagatatcag cgcttcttcc agctggtcca taatggagag ccggaccaca
      361 aagatgatct cagcttcatc ggtgtaattg ctgtcgacta ttttgatgcc ctctgcagag
      421 agtgtgcgaa ggataacatt ggtcaaattg agagggaatt tcactctgta tttttgggtt
      481 agaaggacct ctattatcct tgcagattcg agaacattac gtgcggcatc gcgataggcc
      541 cgtttgagtc cacccgtacc cagctttatg ccgccaaaat accgggttac cacaattcct
      601 gtattgaata attcggcaga tactatgctg tcgtgaatcg gccttccggc tttacccgag
      661 ggctcgccgg cgtcggagta ccggaatatg tccaggcccc gaaagggata aatccggtag
      721 gcgtaacagt tatgattggc gtcatgaaac tcgcttgaga tcttttcgat ttccgcctcg
      781 gcttcctcct gtgactggca tggaaaggcc gaggcaatga attttgatct ctggattttt
      841 atttcggtcc gtgcgggacc cttgatcgtt ttataggagt cttccaatct tccgatctat
      901 tccttcttta ttcctgctta gcttttataa actgatctat caatctctcg aaacgcgcgc
      961 catagatcat atcatacttc tccatatcat ccggaatcag atcttcggct tcttcacggg
     1021 tcttggcgat caattcacgc gccttttcct cggtcaggtc gccgctttga atatcctcgg
     1081 cggtgcggtc aaccagtttt ctcaagttct ctattttttc ctcttcagtc attgtcatga
     1141 ttatctccta taagctgtga tcagagatca attcagcttt actgatgtcc tccaatggaa
     1201 tcgccccata atcgatcaat ccctgatcga caggcaggtc ttttctgccg attgcgaaaa
     1261 gcagcttgcc gagttcacgg gcgcgttccg ctgcatttat ggtaccgcgt gattttttat
     1321 ccagctctcc aacaaccaca gattgggcca gccccacaat tatccggttg cgcgagatca
     1381 gtcgtcccgt tgtaactctg gtatcgggcg agtactcgga aatcaaagct cccttctcca
     1441 gaatctcctc cgatagcttt ttattttccg gagggtagat atggttgaag ccgcatccca
     1501 gtacggcgta tgttctgccc tcggccttga tggctccgat atgcgaggct gtgtcgatcc
     1561 cccgtgccag accggagaca attactgcgc ctttctccgc cagagcgttc gagaaatcta
     1621 ctgctatctc gatcccctcg gccgaggcct gggtggtgcc cacaaccgct atcgtctgtt
     1681 catcctccac tggaaaattg cccttccagt acaggacaaa cggcgggctg ttgatctgcc
     1741 tgagcagttc ggggtaatta tcatcgaata tggtgagggt ctcgatccct tcccggcgat
     1801 atagttcgag gtcctgcttg atgccgtcta aatgtctctc gatttcatgg attaccgcag
     1861 ctttcttttc gctgatccgt ggaaattccg taagctcttc gatatcggcc gagacgatgt
     1921 tttccagcga ctggaaatgc gacaaaagca tctgcaaggt ctttgatccg agcttggcct
     1981 tctcgcgcag ggcgatcaaa agttctttgt tatattcgct cataaaaaag attccaattt
     2041 ctatacaagg tagccctggc atatggacgt gtcaaggcct attacaattt cattagcaca
     2101 ggtaattttg taacatattg atgggtaaat agtttcgaaa aagtcctggc cgtttacatc
     2161 aaaaaatctc ggccgtaaaa atctggattt ccgtggcccg atcctggtag gcattctccg
     2221 gcagacccgc tttcaggcag gtatgcctca aaaatgtttt ccgatcccat ccatattcgg
     2281 tagcaacctg gggcagcaga agtcctgaat ggtaaccgcg cctcatcatg ataccatgct
     2341 tgccgacttc aatttgattc ggatcatcta ttttttcgag gggagagagg accgatattt
     2401 ctatttccag tttatcaact tctttttctg caaggggcgt aaagcgcgga tcatcaaaag
     2461 ccgccgcccg cgccatttct gttatcgttt tatacagagg cttcatcccc acaaccaggc
     2521 cgatacagcc ccgcaggctg ccgcgaatct ttagcgtgac aaaagccccg cagttctcct
     2581 taagtgtttc tgattcaatg ccaagttccg gctcgggctc gttttggaat tttgtcctga
     2641 tggactccag agccagctct ttcagatggt ctttttcttc ctgagtcagg tgcatgattc
     2701 gagttaatcc gaatagatta cagcgcccag atatccaact acctcgcggc tgtcgccggt
     2761 gaccctgccg ctgtccgatt gcccggtaac cgtgatcttg ttcgctccca gcttgcggca
     2821 ggcaagcata gccgcagcca tcggaccgcc gccgcaagcc tcagcctcac ccttggcgat
     2881 cgatttatac aggccctgcg ggtcgaaagc ctcaactttg gcccggatga tcgcatcttt
     2941 ttgtgatgca atctcggcgg tataaaaatg tgacagatcg gtcgaggcca ctatcaatgt
     3001 gttggttccc ttgagtgccg cagccagcgt ttcacccagc gcggtgataa tatcccattc
     3061 ctgctcggcc atgataatcg gcaccagctt gaaatccccc agcatcattt gcaggaacgg
     3121 cagctggacc tcgagtgaat gttcctgtcg ggtgccgccg ccggtatgtc ctttatttga
     3181 aagatacact tttgatggac tgatctggga aattttcgat ccaagttcct gatcgataaa
     3241 aagcttgccg agcggggtca gataggcaga acc
//
LOCUS       DSEB01000150            1854 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1012025, whole genome shotgun sequence.
ACCESSION   DSEB01000150 DSEB01000000
VERSION     DSEB01000150.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1012025"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="ENO07_02770"
     CDS             complement(<1..1001)
                     /locus_tag="ENO07_02770"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:PF02347.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HDS00920.1"
                     /translation="MIFWMLTNTRSLWRNRMSYVSNSESERNDMLKKIGVSKFEELIE
                     EIPESLRLKKPLALDKGKPEFEVVNILRKLAAKNKDSSEMTCFMGGGAYDHYVPAGVD
                     AIISRSEFLTAYTPYQAEVSQGTLQSIYEFQSLVCRLTGMEIANASMYDGASATAEAV
                     LMALNQTGKNKVYLAPGLHPAYKAVIDAYTAGIDLDIQVLKEDSGLVEMSQLNDIDFD
                     DAACVIVQSPNFFGLIENIKSIGSYLKDKKAMFVVVANPMALTILTPPGEADADIAVG
                     EMHVFGNGLNYGGPYVGYFAAQKKYMRKIPGRISASTEDADGRKGYVLTLQTREQHI"
     gene            complement(955..1335)
                     /gene="gcvH"
                     /locus_tag="ENO07_02775"
     CDS             complement(955..1335)
                     /gene="gcvH"
                     /locus_tag="ENO07_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015899710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDS00921.1"
                     /translation="MDFPKDLKYSKEHEWVKVDGDTATIGITDYAQGELGDVVFVELP
                     EAGEEFKQMDAFGTIEAVKAVSELYTPVSGEIVEINEKLEDEAGLVNQDCYGDGWMIK
                     IKLSNPTELDDLLDADKYKELVEK"
     gene            complement(1350..>1854)
                     /locus_tag="ENO07_02780"
     CDS             complement(1350..>1854)
                     /locus_tag="ENO07_02780"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990155.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDS00922.1"
                     /translation="MNTRIQVEHPVTEEVTDVDLIRNQLLIAAGEKMEVRQEDIRFKG
                     HAIECRINAEDPMNNFRPAPGQITSFHTPGGHGIRVDTHAYSKYVIPPFYDSMIAKLI
                     THAKDRDDAIMKMKRALEEFIIEGIPTTIPFHRSVIEDPVFQSGVYSTKYIDGFRWKE
                     KEEAAVK"
BASE COUNT          423 a          503 c          419 g          509 t
ORIGIN      
        1 cttatgtgct gctcgcgagt ctgcagggtc agcacataac ctttccggcc atcagcatcc
       61 tcggtactgg ctgaaattct gccgggaatt ttacgcatgt acttcttttg cgccgcaaaa
      121 taacctacat acggtccgcc gtaattcaat ccgttcccga agacatgcat ctcccccacc
      181 gcaatatcgg catccgcctc gcccggcggt gtcagaattg tcagcgccat cggattggca
      241 accacaacaa acatcgcttt cttgtctttt aggtatgatc cgatgctttt gatattttcg
      301 atcagcccga aaaaattagg cgactgtaca ataacgcagg ccgcatcatc aaaatcgata
      361 tcattcaact ggctcatctc aaccaggcct gaatcttctt tcagtacctg gatatcaaga
      421 tcgattcccg cggtataagc atcaatcaca gctttatagg ccggatgaag tcccggcgcc
      481 agatatactt tgttcttccc ggtttgattc agcgccatca gaaccgcttc ggctgtggcc
      541 gaggcaccgt catacatgga ggcattggca atctccatgc cggtcaaccg gcaaaccagc
      601 gactggaact catagatact ctgcagggtt ccctgcgaaa cttcggcctg gtagggtgtg
      661 tatgcggtta ggaattcgga acgggaaatt atcgcatcaa ctcccgcggg aacgtaatgg
      721 tcgtacgcgc cacctcccat gaaacaggtc atctcagatg aatccttgtt ttttgcggca
      781 agtttgcgga gaatgttgac cacttcgaat tcaggtttgc ctttatcgag cgcaagcggc
      841 ttttttaatc tgaggctttc gggaatctcc tcgatcagct cctcgaattt ggagaccccg
      901 atctttttga gcatatcatt gcgttcagat tcagagttag aaacatagct cattctattt
      961 ctccacaagc tccttgtatt tgtcagcatc caaaagatca tccagctcag ttggattcga
     1021 gagctttatc tttatcatcc agccgtcgcc gtagcaatcc tggttgacca aaccggcctc
     1081 gtcctcgagc ttctcgttga tctcgactat ctcaccggaa accggtgtgt acagttcact
     1141 aacagcttta acggcctcga ttgttccaaa agcatccatc tgcttgaact cttcgccggc
     1201 ttcgggaagt tcgacaaaga caacatcgcc caattcgccc tgagcatagt cggtgattcc
     1261 gatcgtggcc gtgtcgccgt caacctttac ccattcatgc tctttggaat attttagatc
     1321 ctttggaaaa tccatgttac ctccattttt tatttaactg cagcttcttc cttttctttc
     1381 cacctgaatc catctatata ttttgtcgag tacactcccg actgaaatac cggatcctca
     1441 ataaccgacc ggtgaaatgg tattgtggtc ggaatgccct cgatgataaa ctcctccagg
     1501 gcgcgtttca tcttcattat ggcgtcatca cgatccttcg catgggtgat cagtttggcg
     1561 atcattgagt cataaaaggg cgggatcacg tattttgaat aggcatgagt atcgacgcga
     1621 atgccatgcc ccccgggagt atgaaaggag gttatctggc ccggggcggg acggaaattg
     1681 ttcatggggt cctcggcatt gatgcggcac tcgatggcgt gccccttgaa cctgatatcc
     1741 tcctggcgta cttccatctt ttcaccggcg gcaatcagga gctgattgcg aatcaggtca
     1801 acatcggtaa cctcctcggt gaccggatgc tccacctgga tacgggtgtt catc
//
LOCUS       DSEB01000151            2252 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1007775, whole genome shotgun sequence.
ACCESSION   DSEB01000151 DSEB01000000
VERSION     DSEB01000151.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1007775"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..314)
                     /locus_tag="ENO07_02785"
     CDS             complement(<1..314)
                     /locus_tag="ENO07_02785"
                     /inference="COORDINATES: protein motif:HMM:PF13177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00923.1"
                     /translation="MIETAIQPRIGEIFNRLIENNRLASSYLFYGPPGSGKWQAAVEI
                     AAEILSRKNSAYDESAAMRVRKLVHPDLMIVFPMPMPKKIKEKPAEKVKEEYIEHFIR
                     SK"
     gene            complement(364..1467)
                     /locus_tag="ENO07_02790"
     CDS             complement(364..1467)
                     /locus_tag="ENO07_02790"
                     /inference="COORDINATES: protein motif:HMM:PF01551.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00924.1"
                     /translation="MNKFSLTLLTILLLASAYRLIAQEDPRAIDSLSMEKELIDSLLE
                     LKQSEVEQLYLIDEQLNLTSRLIRRLERQMRAMAKELDGLDVLIDSSRVRLEQMRNRL
                     AKNLKSFYINYQPAPAAVFTDGDIRKAARQLHYFKTTVNFMKSQLDSISLMKTELEQN
                     QERLIFTRRQTEKLMERKNVEESILEMRRKDKSRLLSRIDQDVELKSQYLKETQKDQE
                     QLANLTERLQSISHLIDFQSLRGRLIWPVRGKIVRHFGREHDKTTNTETFSPGIQIKV
                     PAGTEVISSAAGIVAHAAYLRGYGNMVIVDHGKGWYTLYGHLSRMSRSVGENVERGEI
                     LGYSGETGSNVGPILFFGIRKRDQSYDPIEWLM"
     gene            complement(1460..>2252)
                     /locus_tag="ENO07_02795"
     CDS             complement(1460..>2252)
                     /locus_tag="ENO07_02795"
                     /inference="COORDINATES: protein motif:HMM:PF02687.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDS00925.1"
                     /translation="KSALPLSLILLIGFAFLLVGYNLQEATQGIEGRYALEIFLKDAA
                     LPEDIDSLQRFLTQQPAFDSLEMVTREDALDRMSNILGEDLTEVLGYNPLPASIIFYP
                     ADSYKSRTYLEILKRQVELFDYVEKGVFAGEWLEELERFNEIFLRITSAFLILVLVAY
                     ILLLYMILNHLWLKHQGTAGKLHLLGMSRFNLRLPVYIWSLISALITSIMGLIILAIA
                     ASIISKYFIAVRYFEPQHVIAIIISFTAISVLLTIFKPMKIPLYE"
BASE COUNT          538 a          568 c          552 g          594 t
ORIGIN      
        1 atcttggacc gtatgaaatg ctctatgtat tcttctttga ctttttcagc tggcttttct
       61 tttatttttt taggcatcgg catggggaaa acgatcatca aatccggatg taccagcttt
      121 cgcacacgca ttgccgctga ctcgtcatac gcgctgttct ttctggatag tatttcggcg
      181 gcgatttcaa ccgccgcctg ccatttgccc gatccgggcg gaccgtaaaa cagataactc
      241 gaggccagac ggttgttttc tatcaggcgg ttgaaaattt cgccgattcg gggctgtatc
      301 gcggtctcga tcatgcagcc aagataagaa aatccgacag ttttgaaaat aacattttga
      361 aaattacatc agccactcga ttggatcgta agactggtct cttttgcgta tcccaaaaaa
      421 tagtattggg ccgacatttg atccggtctc accggaataa cccaggattt caccgcgttc
      481 gacattttct ccgacagagc ggctcattcg ggacagatgc ccatagagag tgtaccagcc
      541 cttgccgtga tcgacaatga ccatgttgcc gtacccgcga aggtaggcgg catgagcgac
      601 aattccagcc gccgaggaaa taacctcggt accggcgggg actttgatct gtattcccgg
      661 cgaaaatgtt tcagtatttg tggttttatc atgttcccgt ccgaaatgcc tgactatttt
      721 ccccctgacc ggccagatca gcctgcctcg cagcgattgg aaatcgatca gatgagagat
      781 cgattgaagt ctttcggtca gattggcgag ctgctcctgg tccttctgag tctctttcag
      841 gtactggctc ttcagttcga cgtcctgatc gatacgggaa agcagacgcg atttatcctt
      901 cctgcgcatc tccagtatgc tctcctcaac atttttgcgc tccatcaatt tttcagtctg
      961 gcggcgggta aagatcaggc gttcctggtt ctgttccagt tcggttttca tcaggctgat
     1021 gctgtccagc tgcgatttca tgaagttgac cgttgtcttg aaatagtgaa gctgccgggc
     1081 ggccttgcgg atatcgccgt ccgtgaagac ggcggccggg gccggctgat aatttatata
     1141 gaagctcttg agattctttg ccagcctgtt tctcatttgt tccagcctga ccctgctgga
     1201 atcaatcagg acgtccagac cgtcgagttc ctttgccatc gccctcatct ggcgctccaa
     1261 ccttcttatc aggcgggagg tgagattcaa ctgctcatcg atcagataaa gctgttccac
     1321 ctcggactgc ttcaattcca gaagactgtc gatcagctcc ttctccatgc tcagtgaatc
     1381 aattgcgcgg ggatcttcct gggcaatcag tctatatgcc gaagcgagaa gcaggatcgt
     1441 taataatgtg agcgagaact tattcatata acggaatctt catcggttta aaaattgtca
     1501 gcaataccga aatggctgta aacgatatta taatagcgat aacatgctgt ggttcgaaat
     1561 agcggactgc tataaaatat tttgagatga tcgatgcggc aatcgccagg attatcagac
     1621 ccataatact cgtgataaga gcggaaatca atgaccagat atagaccggc agcctcaggt
     1681 taaagcggga catgcccaaa aggtgcagct tgcctgcggt tccctggtgt ttgagccaga
     1741 ggtgatttaa gatcatatac aggagcagta tgtaggcgac cagaaccagt atcaggaatg
     1801 cgctggttat acgcagaaaa atttcgttga atctctccag ttcttccagc cactccccgg
     1861 caaacacacc tttttcgaca tagtcgaata actccacctg ccgtttaaga atttccaggt
     1921 aggtgcggct tttatagctg tcggcgggat agaatatgat actggccggg agagggttgt
     1981 agcccagcac ctcggtcaga tcctccccca gtatattgga cattcgatcc agcgcatcct
     2041 cacgcgtaac catctccagg ctgtcaaagg cgggctgctg ggtcagaaag cgctgaagcg
     2101 aatcgatatc ctccggaagc gccgcatcct tcaggaatat ttccagagca tatcggcctt
     2161 caataccctg tgtagcctcc tggagattgt agcccaccag cagaaatgcg aaaccgatca
     2221 ggaggatcag cgacagcggc agggccgatt tc
//
LOCUS       DSEB01000152            3705 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002555, whole genome shotgun sequence.
ACCESSION   DSEB01000152 DSEB01000000
VERSION     DSEB01000152.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002555"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            323..1654
                     /locus_tag="ENO07_02800"
     CDS             323..1654
                     /locus_tag="ENO07_02800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDS00926.1"
                     /translation="MNNSDLRKEINRLAWPIIASMVLIRGVGIADVIILGHTGKEPLA
                     AIGLGQQITFVGMALAYALSVGVNVMVSYYTGSKNPEKRTRIINSSIWLSVVVGLIMI
                     MLGENFSRPLAAFMGAEDQVFDLVWKYLRLIWLFYTFRVFVYNLTAIFQGTGDTRTPM
                     YVVSITNVVHIIVAYILVYGKFGVPSIGIEGAAWATVVSDFLGASIMYIIALKRGLVN
                     FKLGWATPVQIRRLWNLSLPVIGERTLVSTAIMLFNAIVLHYSVAAYAATQILVNIEA
                     FSFLPGMAYMQTAQIMVGQNLGAKNHERAVRSGYQAGWIALSIMSLFGLTFLLFPSFW
                     VSIFTNDPEVVPYGLYVCKMAAALQPVLAITMVFGGALRGAGETRWVMYISFIGAWIV
                     RLSFASFFAYILEWSVHPVWWAMFVDWASRSMLALWRYRKGGWKLSEEDDS"
     gene            1675..2469
                     /locus_tag="ENO07_02805"
     CDS             1675..2469
                     /locus_tag="ENO07_02805"
                     /inference="COORDINATES: protein motif:HMM:PF13489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDS00927.1"
                     /translation="MSKNKLYLHPKYYDIAFDFRDLRKEHNFMTRLYQKTVGRSPRSI
                     VDIACGPAYHAIYFSKKNGIDYSYGLDLSEAMVAYAREKNEIEGGTARIVRGDMIDFK
                     LPRKVDMAMCMIASIHMLTTNEELLKHLDSVARNLNPDGLYIVELQHPRDNFEEQEDD
                     TNIWDMELNGTKVFVQWGTDDDPFDPITQVHETSVTMQVKEKGEKRKTFTFTDPYRII
                     PYQEFMLLIKCSGKFKYLGSYGKFDLRKKMDTSEASWRMIAVLQKK"
     gene            complement(2479..3255)
                     /locus_tag="ENO07_02810"
                     /pseudo
     CDS             complement(2479..3255)
                     /locus_tag="ENO07_02810"
                     /inference="COORDINATES: protein motif:HMM:PRK13321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing start; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
BASE COUNT          874 a          946 c          906 g          979 t
ORIGIN      
        1 attgtattga atactttaat acacaattta gtaaattcgg cctatttttc aaacccgttt
       61 ttatcggcat tgtttcctga tttggtcagg attgacgggg atacgggcat tttgtatgat
      121 agagccgcac agtgagtctg gccaatctga aaggcttttt cattcaagcc agccgttgaa
      181 attctcccgg acatccggca tatgagcgat ttttgatcgc ccaggagttt tatgtattga
      241 atgaacaatc ctgataatac tggtgagaat agcaatttta tccggttgac attcaaaagg
      301 gaaaaagcga ttttgacatc ttatgaacaa cagcgacctg cgcaaagaaa tcaaccggtt
      361 ggcctggccg attatcgcct ccatggtact catcaggggt gtagggattg ccgatgtgat
      421 cattctcggc cataccggca aagaaccgct ggcagctatc ggactcggac agcagataac
      481 gtttgtgggc atggcgctgg cgtatgcgct ttcggttggc gttaatgtca tggtctccta
      541 ttacacggga tccaagaatc cggaaaaacg tacacgcatt ataaacagct cgatctggct
      601 ttcggtagtg gtagggctga tcatgatcat gctgggcgag aatttctcgc gcccgctggc
      661 ggccttcatg ggcgcggagg atcaggtttt cgacctggtc tggaaatacc tgcgcctgat
      721 ctggcttttc tacacattcc gggtgttcgt ctataatctg acggccatat tccagggcac
      781 cggggacacc cggaccccca tgtacgtggt ctcaatcacc aatgtagtgc atataatagt
      841 ggcatatatt ctggtctacg gcaaattcgg ggtaccttcc atcggtatcg agggcgcggc
      901 ctgggccact gttgtttctg actttttggg tgcctcaatt atgtatataa ttgccctgaa
      961 acgaggcctg gtcaacttta agttgggctg ggcgacaccg gtccaaattc gcaggctctg
     1021 gaatctttcc cttcccgtaa tcggtgaacg cacactcgta tccaccgcga tcatgctctt
     1081 taatgctatc gtactgcatt attcggtggc tgcctatgcc gctacacaga tattagtaaa
     1141 tatcgaggcc ttttcgtttt tgccgggcat ggcatatatg cagaccgccc agataatggt
     1201 gggacagaat ctcggcgcca aaaatcatga gcgcgcggtg cgctccgggt atcaggcggg
     1261 atggatcgcg ctttcgatta tgagtctttt cggcctgacc tttctattat tcccctcttt
     1321 ctgggtgagc atatttacga atgatcccga ggtcgtcccc tatggccttt atgtctgcaa
     1381 gatggccgcg gccctgcagc ctgtactggc aatcaccatg gtcttcgggg gagcattgag
     1441 aggcgcagga gagaccagat gggtgatgta tatatctttt atcggcgcct ggatagtaag
     1501 gctttccttt gcctcatttt ttgcttatat actggaatgg agtgttcatc cggtctggtg
     1561 ggccatgttt gtggactggg ccagccggtc catgctggcg ctctggcgat atcgcaaagg
     1621 gggctggaaa ctttctgaag aggacgacag ttagaatacg taaggagaag aactatgtca
     1681 aagaataaac tgtacctgca cccgaaatac tatgacatcg ctttcgattt ccgtgatctg
     1741 cgcaaggaac acaatttcat gacgagactg taccagaaaa ctgtgggacg atctccccgc
     1801 tcgattgtag atattgcctg cggcccggct taccacgcga tctatttttc caaaaagaac
     1861 ggcatcgatt attcctatgg tctcgatctt tcggaggcta tggtcgcata cgcccgcgag
     1921 aaaaacgaaa ttgaaggcgg gactgcccgg atcgtccggg gcgatatgat tgacttcaaa
     1981 ctccccagaa aagtcgatat ggccatgtgc atgatcgcct ctattcatat gctcacaacc
     2041 aatgaggaac tgcttaaaca cctcgattca gtggccagga accttaatcc cgacggcctc
     2101 tatattgtcg aactccagca tccccgtgac aactttgagg agcaggagga tgataccaat
     2161 atctgggaca tggaactcaa tggaaccaaa gtctttgtgc agtggggtac cgatgacgat
     2221 cctttcgacc cgataaccca ggttcatgag accagcgtta ccatgcaggt caaggagaag
     2281 ggcgagaagc gcaagacctt cacctttacc gatccctata gaattattcc ctatcaggag
     2341 tttatgctcc tgatcaaatg ctccggaaag ttcaaatacc tgggttcata cggaaaattt
     2401 gacctgcgca agaagatgga tacctctgag gcgagctggc ggatgatcgc agtcttgcag
     2461 aagaaataat atcaccgctc aatatgattg gccggttata ataccgtatg ccatttcgag
     2521 accgtcaagc accagagatt cgttaaccac atttattacc cgggtatgtt tcgaaaccaa
     2581 ctcggcatat ccgccggtgg cgatgactgt aaatgatgtg cccagttcct cggttatctt
     2641 caatacgaat ccctcaacca tcgcaaccgt cccgtaaaac aggccggatt ttatcgaatc
     2701 ctcggttgtc ctcccgatta cattcccggg cggggaaaca tccacgttaa ataactgtgc
     2761 ggcccgcctg gacagcgaat gtgctgaggt ctgaaccccg ggacatatca cgccccccag
     2821 atattcacca tctttattaa tcacatcaaa tgtcgtggcg gtgccgaaat cgattacgat
     2881 acagggtccc ccgtagagct tgtgcgccgc cagcgcaccc atgatgcggt cggttcccac
     2941 ttcctttgga ttatcataga ggatcttgat tcccagcggc gacatgccgt caaccaccac
     3001 agcttcgatc ccgaaatact ggcgggacat ctctatcagc ctgccggttg cgtgcggtac
     3061 cacagacccg attaccaccg ccttgatatc ctccggggca tgggagcccc gttttataaa
     3121 ccagtccact gccatcgcac actccgatga tgttcccagt tcgtgcgtgg taaaacggcc
     3181 ctggctcata agcctcttat ccctgtaaat gccgaatacg gtgttggtat tcccgcagtc
     3241 tatggctaac agcatgctcc agccttatac cgttcttatt attaatgtat ccctcagggc
     3301 cagttacgac cctgaggatt taatgcgatt ttctatctat ctgctgacaa ttttacagca
     3361 attattggcc gcccagaata ataggcaggc cgtcagccgc atttccgatg acgaccacct
     3421 tggtattggg agagttggcc agaagctggg tggcctcgat ccccttccag cgcaggaaat
     3481 ccttggtcag tccggcggat acgatctgct ggaattttcg aataccctcg gcctcgatta
     3541 ctttacgttc cgcctcctgt tcctccttca tcaatgtgaa ttccatccgt tgctggtcct
     3601 gatcggcggc cagcttctca tttattgcat cagatatttt cttggggaga actacatctc
     3661 tgattatcgt attatcgaca ttgatgaatt tacccgccat caggg
//
LOCUS       DSEB01000153            6735 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000688, whole genome shotgun sequence.
ACCESSION   DSEB01000153 DSEB01000000
VERSION     DSEB01000153.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6735)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6735)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6735
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000688"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(324..1181)
                     /locus_tag="ENO07_02815"
     CDS             complement(324..1181)
                     /locus_tag="ENO07_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00928.1"
                     /translation="MNWNPKFTATITLSFLLIMSLRLPADNHLETSLNIDDYLTELPE
                     NLVLNEDTPQEYRITTVWHNRDLQGNATAKFIISGQYTRALEGDSVQWNNVMIEIFPD
                     PSKAASDTAYQEFMEGFKYKSPEDIARYDFFKDFPSDESTHLLRTLIWDAVAFEVFGW
                     NYFDQLELNKAIVPSDLVDFDAPMADWGTITMKNLKLIWVGVSELNNELCAVIDYESF
                     VNPVNSSSPGMTVQGRSLYWGIIYISLEDKQIEYATMNEDVIMEITTLTNPENRVNIQ
                     REVKFEKLR"
     gene            complement(1299..2351)
                     /locus_tag="ENO07_02820"
     CDS             complement(1299..2351)
                     /locus_tag="ENO07_02820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00929.1"
                     /translation="MLIFDHFIVYLTIEGVHAMGPTDSSFANWNIFESYLNLYWRDIV
                     MQRIFYLIALAVMTAFMGLAMTDLHADYLCGDANTDDMLNVSDAVFIINHVFIGGAAP
                     DPACIGNANEDAALNVSDAVFIINHVFIGGPAPGAYCCCPSTVMDYDGNVYQTVRIGD
                     QCWMMENLKVTHYRNGDAIPGVKDGGVWAGLTTGAWCNYNNNIANTDVYGRIYNWHAV
                     NDSRNIAPAGWHVPSDTEWKQLEMYLGMTQADADAYGWRGKGQGGMLKEAGTVHWQDP
                     NTGATNSSGFTALPGGCRSEFGDFINIYSFAYFWTSTGYASNVAWCRSLYSGTADIAR
                     YHNYREIGYSVRCVKD"
     gene            3016..5094
                     /gene="fusA"
                     /locus_tag="ENO07_02825"
     CDS             3016..5094
                     /gene="fusA"
                     /locus_tag="ENO07_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_953903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDS00930.1"
                     /translation="MTREMTHKIRNVGIVAHIDAGKTTTTERILYFTGKTHKIGEVHD
                     GQAVMDFMKQEQERGITISSAAITCDWQDHQINIIDTPGHIDFTLEVERSLRILDGIA
                     MVFCAVGGVEPQSETVWHQADRYKVPRIAFVNKMDRQGADLFQTVQMMDEMLAANPVL
                     FQIPIGEEADFRGVIDLVNMKAVTFKDFVRTFEDIPADMRELAEKWHQKLIEKLADYD
                     DNLMEKFLDEKFITPADIKAAARNAVTRLFITPVFCGAAFKNVGVRLLLDAIVDYLPS
                     PIDRGSVTGFDVDDSEKAITRRPGIDRPFSSLAFKIINDSYVGQQTFIRVYSGKLESG
                     SYIYNTTKRKRERIGRIMRIHANKREEVDALKAGDIGALIGLKFTTTGDTLCDEEHPI
                     LLENIHYPETVLDMKIKPANMKDRDRLSLALNRLALEDPSFKTHFDQETEETVISGMG
                     ELHLDIIVDRLKTEHKLDVEVGAPAVAFRETITREVRHDYRLKKQTGGKGQFAHIVFR
                     LEPNRGNGIEFVEHVKGGNIPREYIPAIEKGFRETVQNGLMADYPMVDVKFVLLDGSY
                     HEVDSSEMAFRTATGQALKQIIHKAGPQLLEPIMKIEIGTPDEYMGDIIGDLNRRRGK
                     VVNMRRFRKGAQKINGFVPLMEAFGYSSTLRTLSSGRANYSMEFDHYSPLPKEIEDKV
                     VTDQKQKKAS"
     gene            5223..>6735
                     /locus_tag="ENO07_02830"
     CDS             5223..>6735
                     /locus_tag="ENO07_02830"
                     /inference="COORDINATES: protein motif:HMM:PF02897.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDS00931.1"
                     /translation="MTRPIFSAGCHKGQRLIYYAIFAFLTILLLHCSQGDKQAAKYDY
                     PPARRADVVDNYHGTQVADPYRWLEDPNSEETQKWVKAQNELFHEFVNTPVRKKIKKR
                     LTELWDYPKYGSPCKAGGRYFFWKNDGLQNQDVLYMIESLPDKPVVVLDPNKMSEDGT
                     VSVELKDFSTDGKYFVYAVKVSGSDEEVLKIRDIDSGTDLDESIEFTKFSAVGWKHDN
                     SGFFYDRYPDPKTVPAEDVNKFNRVYFHKLGTPQSEDELIFEMPSDKDLGFAPRVSDD
                     GQYLILNVWHGTDNENRIYYRPVGDEGEFTRLLDEADASYQFVNNIGTDFYFNTDLDA
                     PLNRVVKIDIKNPARDNWIDIIPEQENAIESVNMIDKKLVVVYLEDAHDMLKIFDLDG
                     KLTAEPELPTIGSMHSLKGKRDHREMFFKFSSFLYPAYVYRYDFEDSELSIFRKSEVD
                     FDPEDYTTRQVFATSKDGTRIPLFITHKKSMMPEGTNPVLLYGYGGFNNNMTPY"
BASE COUNT         1834 a         1522 c         1661 g         1718 t
ORIGIN      
        1 aaatagtagt gcaatagcaa gaggaaaaac catcatgact gcaattctaa acaatggcat
       61 ccaggtaaga tgtatttctg cactgccttc aagctcacat tcatcttttc gcggttgcat
      121 tttccctccc tatcagattg tctaaatcaa ttcagattca ttataaatga tccgctaaat
      181 gtaggttgtc ctacatgcaa atgttatgac tatttgaatg cggggctctt accaataata
      241 taatcaatgg atataatatg caagtatttt tgatatttaa aaatagaaga tttttgaagg
      301 tgcatgcttg ggtattttgg ccactaccgc agcttctcga atttgacctc gcgctggatg
      361 ttcacgcgat tctcgggatt ggtcagagtg gtgatttcca ttatcacatc ctcgttcata
      421 gtggcgtatt cgatctgctt gtcctcaaga gagatgtata tgattcccca gtagagcgag
      481 cgtccctgta ctgtcatgcc gggcgaagag gagtttacag gatttacaaa ggactcataa
      541 tctatcaccg cacacagctc attgtttagc tccgatactc ctacccaaat aagtttcagg
      601 tttttcattg tgatggttcc ccagtcggcc atgggcgcat cgaaatctac caggtctgag
      661 ggaacgattg ccttgttcag ctcaagctga tcgaagtaat tccatccgaa tacttcaaaa
      721 gcgacggcat cccaaattaa agtcctcagg agatgcgtgc tttcatcaga cggaaagtcc
      781 ttgaagaaat cgtatctggc aatatcctca ggtgatttat atttgaaccc ttccataaat
      841 tcctgatagg ccgtatccga agcggccttt gatggatcag gaaaaatttc tatcatgaca
      901 ttattccatt gcacactatc cccttccagt gctcgtgtat attgtccgga gattatgaat
      961 tttgcggtag cgttgccttg aagatcgcgg ttatgccaga cagtggtgat tctatattcc
     1021 tggggcgtat cctcgttcag aacaaggttc tcaggtaatt ctgtgagata atcatcaata
     1081 ttcagggaag tctcaagatg gttatctgca gggagtctca ggctcattat gagcagaaaa
     1141 gatagtgtta tggtggcagt aaattttggg ttccagttca tcttgataat ccctccagaa
     1201 aaataaattc aaatgtgcgg tccagtgcat tccaccagac caatatagga aatttttaat
     1261 caagaggaat tgctttattt ccgcagacag atagccagct aatccttgac acagcgtacg
     1321 gaatagccga tttccctgta attgtggtaa cgggcgatgt ccgcggtgcc ggaatagagg
     1381 cttcggcacc aggccacatt agaggcatat cccgtggagg tccaaaagta agcaaagcta
     1441 tatatgttga tgaagtctcc gaattcgctg cggcatccgc cgggcagcgc tgtaaaaccg
     1501 cttgagttgg tagcgcctgt gttgggatct tgccagtgaa ctgtgccagc ttcctttaac
     1561 atgccgccct gacctttacc ccgccagccg taagcatcgg catcggcctg ggtcataccc
     1621 aggtacatct ccaactgttt ccactcggta tctgacggca catgccagcc tgccggggct
     1681 atattgcggc tgtcattgac agcatgccag ttatatattc tgccataaac atcagtattg
     1741 gcgatattgt tattatagtt gcaccaggct ccggtcgtga ggcctgccca tacgccgccg
     1801 tcctttacgc cgggaatcgc atcaccgtta cgataatgag tgaccttcaa attctccatc
     1861 atccagcact gatcaccaat ccggactgtc tgatagacat tgccgtcgta gtccattacc
     1921 gttgacggac aacagcaata ggcaccggga gccggtccgc cgatgaaaac gtgattgatt
     1981 atgaaaacag catcactgac attgagcgca gcatcctcgt tggcattgcc aatgcaggca
     2041 ggatcaggcg ctgcgccgcc gataaagaca tgattgatga taaacactgc gtcactcaca
     2101 ttgagcatgt cgtcggtgtt agcgtcaccg cacaagtagt cagcatgtaa atcagtcatg
     2161 gccaagccca taaatgcggt cataactgcg agggcaatta aatagaatat cctctgcatg
     2221 acgatatccc tccaatataa atttaaataa ctttcaaaaa tattccaatt tgcgaagctt
     2281 gaatccgtcg gccccattgc gtgtacgccc tctattgtta aatatactat gaagtgatca
     2341 aaaatcaaca tttttgtcaa atattgcgga aattaggggg cgggaggctc catggccctc
     2401 tgtgggtcag gcgggggaga ggccgccctg agcgtgctaa tcccagtcgt cgtcgcctcg
     2461 gccgtcgccg ataacaccat gcttgattat cgaacggata agcaaaatcg atatgatgag
     2521 gcagactatg aaaactatca tgatagaaat atagtctcta tctatgacag gacaacgatt
     2581 ttcttattaa ggtacgatca ttttggaagt tccgatttgg ttcattttca cagagaaccc
     2641 acagctaaag ctgtggggca ccccggcgac gtgatgatct cttaaagtat gcgatgcaga
     2701 aattgtgaag attctcacgg tcgtcctcga ccaaggtcgg gactccctca gaatgacgcg
     2761 tattggcctt tttcgctgtc ccaatacttt ttcaataagt ccctaaagcg gcggagcacc
     2821 cagattaatg taaaaaatca accaataatt atcctatttt tctcgaatcc agggaacata
     2881 agggccgatt atacgttgaa caccactgtt ttcaggggat acgctaaatt ttaatgaaaa
     2941 tttagcttga tagttgaggg gcatttagat atactcggag tttttaattt cgaggagttt
     3001 tcaggagtta ttgaaatgac gcgagaaatg acacataaga tacggaatgt cgggatcgtt
     3061 gctcatattg atgcgggcaa gactactact acggaacgga ttctgtactt caccggcaag
     3121 acacataaaa tcggcgaggt tcatgacggc caggcggtga tggacttcat gaaacaggaa
     3181 caggaacgag ggatcacgat ctcgtccgcg gcgataacct gcgactggca ggatcaccag
     3241 ataaatataa ttgatacccc gggacacatc gactttactc tcgaggtgga gcgttcgctt
     3301 agaattctgg atggtatcgc gatggtattc tgcgcggtgg gcggtgtgga acctcagagc
     3361 gagactgtct ggcatcaggc cgaccgctac aaggtcccgc gcatagcgtt cgttaacaag
     3421 atggatcgcc agggcgccga tcttttccag actgtgcaga tgatggatga gatgctggcg
     3481 gccaatccgg tactgttcca gattccgatt ggcgaggaag ccgatttccg cggtgtgatc
     3541 gaccttgtta atatgaaggc ggttacattc aaggattttg tccgtacatt tgaagatatt
     3601 cccgctgata tgcgggagct ggccgagaaa tggcaccaga agctgatcga gaaactggcg
     3661 gattatgacg ataatttgat ggaaaaattt ttagacgaga aattcataac tccagcggat
     3721 atcaaggccg ctgccagaaa tgcggtaaca cgcttattta ttacccccgt attctgcggt
     3781 gccgcattca agaatgtcgg tgtcaggctg cttctggatg ccatagtcga ctacctgcca
     3841 tccccgatcg atcgcggcag tgtaaccggg tttgatgttg atgattcgga gaaggctatt
     3901 acccgcaggc cgggtatcga caggccgttc tcatcgctgg cattcaagat tataaacgac
     3961 agctatgtcg gacagcagac ctttatcagg gtctattccg gcaagctcga gtccggaagc
     4021 tatatctata acacgaccaa gcggaaacgc gagcgtattg gaaggatcat gcgtatccat
     4081 gccaacaagc gcgaggaggt cgatgctttg aaggccggcg atatcggggc gttaatcggc
     4141 ctgaagttca ccacaaccgg tgataccttg tgtgacgagg aacacccgat attgctcgaa
     4201 aatatacact atcccgaaac tgtgctggat atgaagataa agccggccaa tatgaaggac
     4261 cgcgaccgcc tgtcactggc cctcaatcgc ctggcgctgg aggatccctc gttcaagacc
     4321 cattttgacc aggagaccga ggaaacggtg atctccggca tgggcgagct tcatctggat
     4381 atcatagttg accgccttaa gaccgaacat aaactcgatg tcgaggtggg cgcgccggca
     4441 gtagcgtttc gcgagacaat cacacgcgag gtcaggcatg actacaggct caagaaacag
     4501 accggcggta agggccagtt tgctcatata gtattcaggc tggagcctaa tcggggcaac
     4561 ggtattgaat ttgttgagca tgtcaagggc ggaaatattc ctcgcgaata tattccggcc
     4621 atcgagaagg gcttccgcga gacagttcag aacgggctta tggcggatta tccgatggtt
     4681 gatgtcaagt ttgtactgct cgacggcagc tatcatgagg tcgattcgag cgagatggcc
     4741 ttccgcacag cgactggcca ggccctgaag cagataatcc acaaggccgg cccgcagctc
     4801 ctggaaccga taatgaagat tgagatcgga accccggatg aatatatggg tgatattatc
     4861 ggcgacctga accgcaggcg gggcaaggtt gtcaatatgc ggcgtttccg caaaggcgcc
     4921 cagaagatca acggtttcgt gcccttaatg gaggccttcg ggtattcctc cacgctcagg
     4981 acattatcca gcggacgcgc caattattct atggaatttg atcattattc gccgctgccg
     5041 aaggaaattg aggataaggt cgtaaccgat cagaagcaga aaaaagccag ctaaatcaag
     5101 cggcgtatat agattcaaag gcccgattcg ggttcaaggt atatttttta tgccttgccg
     5161 aatgcgggcg tttttatttt attgcattga atctcggcgg aaacaaccag gagaatattc
     5221 agatgacaag accaatcttt tcagcaggat gtcataaagg gcaacgcctt atttattatg
     5281 cgatctttgc attcctgacg atccttttac ttcattgctc gcaaggcgat aagcaagcgg
     5341 caaaatatga ttatcctccc gcgcgacggg ctgatgttgt tgataactat catggcacac
     5401 aggtggccga tccataccgc tggctggagg acccgaattc tgaggaaacc caaaaatggg
     5461 tcaaagccca gaatgagttg tttcatgaat ttgtaaatac tcccgtaaga aagaagataa
     5521 aaaaacgcct gaccgaatta tgggattatc ccaaatacgg ttcgccatgc aaggctggcg
     5581 gacgatactt cttctggaaa aacgacggcc tccagaacca ggacgtgctc tacatgatag
     5641 agtcacttcc cgataagccg gttgtcgtat tggatcccaa taagatgagt gaggatggca
     5701 ctgtttctgt ggaattgaag gatttcagta cggatggcaa atacttcgtg tatgcagtta
     5761 aagtgagcgg gagtgatgaa gaggtattga agatcaggga tattgactca ggcactgatc
     5821 tggatgaaag catagaattt accaagttca gcgctgtggg ctggaaacat gataattcag
     5881 gtttcttcta tgaccgttat cccgatccga aaacagtccc ggcagaagat gtcaataaat
     5941 ttaacagggt ttattttcat aaactgggca caccgcagtc ggaagatgaa ttgatcttcg
     6001 agatgccctc ggataaggac ctgggctttg caccaagggt ttccgatgac gggcagtacc
     6061 ttatcctgaa tgtctggcac ggcaccgata acgaaaatcg gatttattac cgcccagtcg
     6121 gtgatgaggg agaatttacc agactgctcg acgaagcgga tgcatcatat cagttcgtaa
     6181 ataacatcgg aacggatttc tatttcaata ccgatcttga tgcgcctttg aacagggttg
     6241 tgaagataga tatcaaaaat cccgcaaggg ataactggat cgatataata cccgagcagg
     6301 aaaatgccat tgaaagcgtg aatatgatcg ataaaaagct ggttgtcgtg tatctcgaag
     6361 atgcgcatga catgcttaag atatttgacc tggacgggaa attgactgcg gaaccggaac
     6421 ttcccacaat cggatccatg cacagcctga aagggaaacg cgaccatagg gaaatgttct
     6481 tcaaattttc gtcttttctt tatcccgcat atgtctatcg ttatgatttt gaggattcag
     6541 aactgtcgat attccgcaaa tcagaggttg attttgaccc ggaggattat acaaccagac
     6601 aggtctttgc gacatccaaa gatggtacca gaataccgct gtttattact cataaaaaga
     6661 gcatgatgcc ggagggcacc aatccggtac ttctgtatgg gtacggcgga tttaataata
     6721 atatgacccc atatt
//
LOCUS       DSEB01000154            3057 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003954, whole genome shotgun sequence.
ACCESSION   DSEB01000154 DSEB01000000
VERSION     DSEB01000154.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003954"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(4..447)
                     /locus_tag="ENO07_02835"
     CDS             complement(4..447)
                     /locus_tag="ENO07_02835"
                     /inference="COORDINATES: protein motif:HMM:PF04191.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HDS00932.1"
                     /translation="MILLSGRIEALLNFPDSFIPHALGRKAVGSVVAVCGLIFFTLGL
                     GRSLLFLQKKRARKFIKSGVFKYSRNPSSFGIVSTLFGVSFIMDSLGLFIIAFAMLLF
                     FTVTVHFADRIMYETYGEDFSRYKKGTPRFIPNFNRLVCNMFGLE"
     gene            complement(530..2053)
                     /locus_tag="ENO07_02840"
     CDS             complement(530..2053)
                     /locus_tag="ENO07_02840"
                     /inference="COORDINATES: protein motif:HMM:PF05193.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDS00933.1"
                     /translation="MRRKEGQVLKYILIVSILLFAVTCSGGEKNAVSDEPGIIKFVSV
                     PVDRLPVVHFGMVIKSGSASDPQGKEGLAWFTANLLKRGSKSYNREQIEKGLEEIGGQ
                     LDVRVEREAIVITGKTLRENLDKYYAIFSDIILNPKFPSDEVAGLRTDQEQAIKNIIR
                     NDARLCHEAFLRELYDGHHYSHPAQGNLGSAAKLTANDAERFYRKHFVKGNVICGLAG
                     MYSPEFEKKFQDDMNKLKRGMVQDRTEMVAQPAELRVVLVEKPGRDQSQIRIGKMIDY
                     TRRDSLWYTYLAANTYLGQHRESFGRLYTAIRSERGLSYGAYSYHEHFRQSGWSKNPM
                     PLTPFNPQYFSIWTYPKRINTEFAIKMAVHELHKILTGGIEPNQLQKFVSFQVNHFPF
                     LIETAEQQLLMQMEEIYYGQPDFIENFEKKLQSVSHANISDILNQSWSSDALLIVVVT
                     DSAGAMKEELLTQETSLELPSGATEAGLEDVNNRVKNIDLGLEAKDIRIIDAEKLFN"
     gene            complement(2043..3017)
                     /locus_tag="ENO07_02845"
     CDS             complement(2043..3017)
                     /locus_tag="ENO07_02845"
                     /inference="COORDINATES: protein motif:HMM:PF05193.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDS00934.1"
                     /translation="MPEIIAIEADRIRNLDFTNETFRRELGPVKEERRRFVDDDPDGF
                     LEEQIYRLAYTRHTYEHPVIGWQNDLEKNMTFRDAADFREVYYSPNFVTIVIGGSFDT
                     ENVLNLIQQHYGDWPSHLPPIGRIRKEPPQSKERTLDLTWKSPEIPAKAAVAYHIPDL
                     NYMDNDILALQILQRMLFSESGKVRKKLKDELGLVESIRADAEGMKDPGLFLIRMNLT
                     NPENIDSALTVLYSQIDSIKVNGVSKEDLVRAVNSMKAEYLYRLDRPARIVGTIGYYH
                     LVGGDHKLMFEYYEQLGGVTVKDLQDAAKEYLKESNRNVVVLKPADAA"
BASE COUNT          783 a          792 c          696 g          786 t
ORIGIN      
        1 aaattattcc agcccgaaca tattgcatac caggcggttg aaatttggaa taaagcgcgg
       61 tgtgcccttt ttatacctgg agaaatcctc accataagtt tcgtacatga tgcgatcggc
      121 aaaatgcacg gtgacggtga aaaacaacaa catcgcaaat gcaataataa aaagtcccag
      181 ggaatccata ataaatgata ccccgaaaag cgtggacaca atcccgaatg atgaaggatt
      241 gcgtgaatac ttgaaaacgc ccgatttgat aaattttctg gcccgttttt tctgcaggaa
      301 aagaagcgac cttcccagcc ccagcgtaaa gaatatcagc ccgcaaaccg ctaccaccga
      361 tccgaccgcc ttgcgtccaa gagcatgagg gatgaagctg tccgggaaat tcaataacgc
      421 ttctatacgt cccgaaagca agatcatcag gtatacaaag ccgccgcaca gaaacggcag
      481 aaagggtgta taccagacta catcacgatc ccgaaaagaa cgcaactggc tagttgaaaa
      541 gtttttctgc gtcaattatt ctaatatcct tcgcttccag gccaagatca atgttcttga
      601 cccgattatt gacatcctcc agaccggcct cagtggcccc gctgggcaat tccagggatg
      661 tttcctgtgt cagaagctcc tccttcatgg ccccggcgct gtcggtcacg acaactatca
      721 gaagagcatc gcttgaccag ctttgattga gaatatcgga aatatttgca tggctgacag
      781 actgaagttt cttttcaaaa ttctcgatga aatcgggctg gccataataa atctcctcca
      841 tctgcatcaa caattgctgc tcggcggtct cgattagaaa gggaaaatga tttacctgaa
      901 acgagacaaa tttctgaagt tgattcggct ctattccccc ggtcaaaatt ttgtgcaact
      961 catgcacggc catcttaatt gcaaactcgg tgttaatccg tttggggtaa gtccagatag
     1021 agaaatattg aggattaaag ggtgtcagtg gcataggatt tttggaccat ccggactgcc
     1081 tgaaatgctc atgatagctg taggcaccat acgacaggcc ccgttctgaa cggatcgcgg
     1141 tatagagcct gccgaatgat tcgcgatgct gccccaggta tgtgttggcg gccagatagg
     1201 tgtaccagag gctgtccctg cgggtatagt caatcatttt tccaatacga atttgcgatt
     1261 gatcgcgtcc cggcttttcg acgagcacca ccctcaattc agcaggctgc gcgaccatct
     1321 ctgtccggtc ctgcaccata ccccgtttaa gcttattcat atcatcctga aatttctttt
     1381 caaactcggg ggaatacata ccggccaatc cgcagatgac attgcctttt acaaaatgct
     1441 tcctgtaaaa ccgttcagca tcatttgccg taagcttcgc cgcgctgccc aggttaccct
     1501 gcgccggatg ggaatagtgg tggccgtcat acaattctcg tagaaaagcc tcatgacaca
     1561 aacgggcgtc attgcggata atattcttta tggcctgctc ctgatcggtt ctgagaccgg
     1621 cgacttcatc cgatggaaat ttcggattta gaataatatc agagaatatc gcgtagtatt
     1681 tatcgaggtt ctcccgcaaa gttttaccgg ttatgacaat agcctcgcgc tcgacccgca
     1741 catcaagctg cccgccgatc tcctccagcc ctttttctat ctgctcacga ttgtaagatt
     1801 tcgatccgcg cttgaggaga ttggcggtaa accaggccag tccctccttg ccttggggat
     1861 ctgatgccga tccggacttt atgaccatgc cgaagtgcac cacaggcagc cggtcaaccg
     1921 gaacgctgac gaattttatg attcccggtt catcggatac tgcgttcttt tcgccgccgg
     1981 agcatgttac agcaaacaga agtatagaaa caattagaat gtatttcagc acctggccct
     2041 ccttacgccg catccgcggg cttcagcaca accacattgc gatttgattc ttttagatac
     2101 tccttggcag catcctgcag atctttaact gtaacgccgc caagctgttc gtaatattcg
     2161 aacatcagtt tatgatctcc acccaccaga tgatagtagc ctattgtccc cactattcgt
     2221 gccggccggt cgaggcgata caggtactcg gccttcatgc tgttgacagc gcgcaccaaa
     2281 tcttccttag agacaccgtt gaccttgatg ctgtctatct ggctgtatag aacggtcagg
     2341 gctgaatcta tattttccgg attagtcaga ttcatgcgga tcagaaacag tccagggtcc
     2401 ttcataccct cagcatcggc ccgaatcgat tccaccagac ccagctcatc tttaagtttt
     2461 ttccgcacct tccccgactc ggagaagagc attctctgca gaatctgaag cgccaggatg
     2521 tcattgtcca tatagttcag gtcggggata tgataggcta cagcagcctt ggccggtatc
     2581 tccggggatt tccaagtaag atccaaggtc ctttctttgc tctggggcgg ttccttgcgg
     2641 attctgccga tggggggcag atgtgagggc cagtcaccat aatgctgctg gatcaggttg
     2701 aggacgtttt cagtatcaaa gctcccaccg atcactattg taacaaaatt tggagaatag
     2761 taaacctctc gaaaatcagc ggcatcgcgg aaagtcatat tcttctccag gtcattctgc
     2821 cagcctataa ccggatgctc gtatgtatgc cgggtgtaag ccaatctata aatttgttct
     2881 tccaagaaac catcgggatc gtcatccaca aaacgcctgc gttcctcctt gacggggcca
     2941 agttcgcgcc ggaaggtttc atttgtgaag tcgaggttgc ggattctatc tgcctctata
     3001 gcaatgattt ccggcaaaaa ctcagccctg gcattaacat aatagcaggt ataatcc
//
LOCUS       DSEB01000155            2761 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004958, whole genome shotgun sequence.
ACCESSION   DSEB01000155 DSEB01000000
VERSION     DSEB01000155.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004958"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1787)
                     /locus_tag="ENO07_02850"
     CDS             complement(<1..1787)
                     /locus_tag="ENO07_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00935.1"
                     /translation="MRRLFILFFMLCLIITGCSGNDSSGPSDNDHDNLLASATIGSEG
                     GTLSSDDISIEIPSGSLDMATELNLYLSGSGEPFAEQGVSPLFRLEGLPQGFDGNLHI
                     AIRYDGVLNAESFIAMGEEFYLVEDDDSGSVYSLFQAADSSGFLLGDIPVISPDARTH
                     GKLNAVSDGAGLIIYLLGVSDYLTLQDGDFRIRYPRILQENAEQLKLFLDQALNTIEY
                     MQFQIPDENTTLPINVLIREKSLGSFAQYIPREAHSYYTDNQYTSIEVREQDMGTADY
                     PEMKLDIGNELYLLVAAMHNRSTLDRLPLSWFDYAGSTWFQCFMSADPDTFVPDLYLE
                     NSTCLLDSFDMRPAAPEYQHLLHGRGMGAFIKFMYSRFGPSVLPYIYVERKEHEISAW
                     RTIFGNYVTVINQPHAQWYPAFIKQLINEEIYDLGGQLSSRLLDKTYHEYDIDSDANQ
                     NATFPFGATALESKIFKFGLNNPDFADGDQLQITVYDKSMLADSMHLIVFKRKGTAVE
                     YIGDGSSVIVDSILGLQQDGWDIFAVAVNSIAGSLNDATEYDYYLNIEVKEAPSINIA
                     RVVINVIVDAYFRDSGDTSYLKPDFNLYWV"
     gene            1981..>2761
                     /locus_tag="ENO07_02855"
     CDS             1981..>2761
                     /locus_tag="ENO07_02855"
                     /inference="COORDINATES: protein motif:HMM:PF07676.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00936.1"
                     /translation="MKRLSTILIVIMTALTLNAQEESKPFLRYPALSPDGETVCFSCK
                     GDLWLVSSEGGQATRLTVHPADDIMPQFSPDGRMILFTSERYDNRDIYIIPVTGGEPE
                     RLTYYTGSDYGTGWTPDGDSVIFYSLRQFNYDVFKMSINGGTPVAMTGGHWDGEYAGK
                     VSPDGKKLIFNNGSGRYRWWKANFAGNTNSDIWMLDRTSEEFTLDRITSDDGHDLWPV
                     YDQASEVIYFVANRDSTVANLWKKDLNSGAESQLTDYSDDGA"
BASE COUNT          750 a          655 c          653 g          703 t
ORIGIN      
        1 acccaatata gattaaaatc aggctttaga taggaagtgt cacccgaatc cctgaaatag
       61 gcatctacta taacgtttat gacgacgcga gcaatattta tgctcggcgc ttctttaacc
      121 tcaatattca ggtaataatc atattcggta gcgtcattca agcttccggc gattgaatta
      181 actgcaacag caaaaatatc ccagccatcc tgctggagtc ccagaatgct atcgacaata
      241 acgctggagc catccccgat gtactccact gctgtacctt ttctcttaaa gacaatcaga
      301 tgcatactgt cggccagcat gctcttatca tagacagtaa tttgcagctg atcaccatcg
      361 gcgaaatccg gattattcag cccgaatttg aatattttgg attcgagggc ggttgccccg
      421 aacggaaaag tggcgttctg attagcatct gaatcgatgt cgtattcgtg ataagtctta
      481 tccagcaaac gcgaggagag ctgaccgcca agatcgtaaa tttcttcatt tatcaattgc
      541 ttgataaagg ccggatacca ttgggcatgc ggctgattta tgacagtgac ataattcccg
      601 aagattgttc tccacgctga tatttcatgc tctttacgtt cgacgtatat atatggcaga
      661 acactgggtc cgaatcgtga gtacataaat ttgatgaagg cgcccatgcc ccggccgtga
      721 agcagatgct gatattctgg tgcggcggga cgcatatcaa acgaatccag taaacaggtt
      781 gaattttcaa gatacaaatc cggaacgaag gtatcgggat cggcggacat gaaacactga
      841 aaccatgtgc tcccggcata gtcaaaccat gacagcggca gtctgtcaag cgtgctcctg
      901 ttgtgcattg cggcgacaag cagatagagc tcattcccga tatcaagttt catttcagga
      961 taatcggctg tacccatatc ctgttcgcgg acctctatcg atgtgtattg gttatcggta
     1021 tagtaagagt gcgcttctct ggggatatat tgcgcaaaac ttcccaaaga cttctcccgg
     1081 atgagcacat taatcggcag cgttgtgttc tcatcgggaa tctggaattg catatattct
     1141 atggtattta aagcctgatc caggaagagc ttcaattgtt ccgcattctc ctgcaatatt
     1201 cgcggatatc gaatcctgaa atcgccatcc tgaagagtca ggtaatcgct cacaccaagc
     1261 agatatataa tcagcccggc gccatcggaa acagcattca attttccatg agttctcgca
     1321 tcggggctga tcacaggaat atcgccaagt aaaaatcctg aggaatcggc agcctggaag
     1381 agactgtaca cagagccgga atcatcatct tcgaccagat aaaactcctc acccatggca
     1441 atgaagctct ccgcattaag caccccatca tatctgattg caatatgaag attcccatca
     1501 aagccctgtg gtaaaccctc gagcctgaat aaaggcgata ctccctgttc cgcaaatggt
     1561 tctccgcttc ctgacaaata taagttgagt tcagtggcca tgtcgaggga tccggagggg
     1621 atttcgatcg agatatcatc gcttgaaaga gtgccgccct cgcttcctat cgttgccgag
     1681 gctaagaggt tgtcatggtc attatctgat ggtcccgaag agtcattgcc ggagcatccc
     1741 gtgataatca agcatagcat gaaaaataaa ataaataatc tgcgcatggt cgtcctccgg
     1801 tttcggcatc ctcccgcggc aagccaagaa atatgcaggt atgcaacact ataagataaa
     1861 caccgatacc aatttagtca attctattct gcagcgcagg aggctcgccg ttagcggttg
     1921 acagatgacc gcttttctcg taatttcatg gaattgatta tttacggaaa ggattgattc
     1981 atgaaacgct taagcaccat tctgattgtc atcatgacag cgctgacact taacgcacag
     2041 gaggaatcga aaccgttctt gagatatccg gccctgtctc cggacgggga gactgtatgc
     2101 ttctcatgta agggtgacct ctggctggta tcctcagagg gagggcaggc cacaaggctg
     2161 acagtgcatc cggcagatga tatcatgccc cagttctcgc ctgatggacg catgattctg
     2221 tttacctctg aaagatacga caatcgcgat atttatataa tcccggtgac cggtggtgaa
     2281 cctgaaagac tgacatatta taccggttcc gattacggca ccggctggac gccggatggc
     2341 gacagcgtaa tattctattc actcaggcaa tttaattatg atgtcttcaa gatgtcgata
     2401 aatggcggca ctccggtggc catgacaggc ggacattggg acggcgaata tgccggcaag
     2461 gtttcacctg acggaaagaa gctcattttc aataatggtt cgggacgtta ccgctggtgg
     2521 aaagccaact ttgccggtaa caccaacagc gatatctgga tgctggatag aaccagtgag
     2581 gaatttactc tcgaccggat aacctcggac gatggccatg atctctggcc ggtctatgat
     2641 caggccagtg aggtgattta ttttgtcgcc aacagggata gcactgtagc caatctctgg
     2701 aagaaggact tgaattccgg ggccgagtca cagctcacag attattctga tgatggtgca
     2761 c
//
LOCUS       DSEB01000156            2176 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1008415, whole genome shotgun sequence.
ACCESSION   DSEB01000156 DSEB01000000
VERSION     DSEB01000156.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1008415"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..>2176
                     /locus_tag="ENO07_02860"
     CDS             <1..>2176
                     /locus_tag="ENO07_02860"
                     /inference="COORDINATES: protein motif:HMM:PF00691.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00937.1"
                     /translation="IYITGHSDNTPINPRSRSIYPDNYALSLARARTVAEYLVDGLDL
                     DSSQFTVTGKGPDDPIAEEIIRGRNDLNRRVDIKVISEKITKYQLLDIARGEDSLSVD
                     IIGIRPGESEEAQAAQEESARSFADLNQEWLADDRPGFQWLWPEDNYNPPIPSLKVAI
                     KHYPDRTPRLLLDGKQVSSLNFSETVSGGSDSVAISIWTGIDLREGTNNFSAVLYDRS
                     GRFSVISRTIHYSGPPVGVEVVDSLSRLIADGREPAIIAVRLIDSDGYPARPGVAGEW
                     SVDPPYMARQTVKDLRAHPLLEIAGQNPRYLVGENGIATLELQPSSRSGEALLRFHLL
                     DREEELRVWLRPALKDWMLVGLAEGTVGYNTTRGSRSNLADAGVDDKTYSEGRAACFA
                     RGSIQGDLLLTLALDSRRWNSNPDSNMFRTINPEAFYPLYGDNMMQDYGSSSSRGIFA
                     RLEAANYYAMFGDYSTGLNVTELSRYNRTFNGLKFEGNAERKNFNLFLSRSNQAFVKD
                     EIQGDGTSGPYALSRNNIVVNSERVVIETRDRFQNDVIISRQTMRQFLDYDIDYYSGV
                     IFFKRPVPSRDEQFNPLFITVDYESNDANDRSYNYGGRVGIDLAEESLNVGGTYIHEG
                     RIGGYGHLGSIDATLAVGDGIQLKTEFAASKVKEGTGRSSGNAYLIELERKMKKLSGK
                     IFIRNLDPEFGLGQLSLSERGMRKFGAAATYRLRESLILNGHA"
BASE COUNT          551 a          547 c          582 g          496 t
ORIGIN      
        1 atatatatca ccggccattc cgacaacact cccatcaatc cgaggagccg cagtatttat
       61 cctgacaatt acgccctctc actggcgcgg gcacggacgg ttgctgaata tcttgtggat
      121 gggctcgacc tcgattcatc ccagtttaca gtaacgggca aggggccgga tgatccgatt
      181 gccgaagaaa ttattcgcgg gagaaatgat ctcaaccgcc gggtcgatat taaagtgatt
      241 tcagagaaaa tcacaaaata tcagctcctc gatattgccc gaggtgagga cagtctttcg
      301 gttgacataa tcgggattcg tccgggagaa tcggaggaag cacaggcggc gcaggaagaa
      361 tccgccaggt cctttgccga tctcaatcaa gaatggctcg ctgatgacag gcccggattt
      421 cagtggttgt ggccggagga taactataac ccgcccatac cgagtttaaa agtagctata
      481 aaacattatc ctgatagaac tcccagatta ttgctggatg gcaaacaagt atcgtcgctg
      541 aacttctctg aaacggtcag cggcggatcg gattccgtag caatcagcat ctggacaggc
      601 atagatttac gtgaaggtac gaataatttc tctgccgtgc tgtatgacag atccgggcga
      661 ttttcagtaa tttcccggac catacattac tccggtcctc cggtcggcgt ggaggtcgtt
      721 gattccctct ccaggcttat agccgatgga cgcgaacctg ccatcatcgc cgttcgttta
      781 atcgattcag acggctatcc ggcccggccc ggtgtggccg gagaatggtc tgttgatcct
      841 ccttatatgg cccggcagac ggtaaaagat ctgagggccc atcccctgtt agaaatcgcc
      901 ggacaaaatc ccaggtatct ggtcggcgaa aacggtatcg ccaccctcga gctgcagccc
      961 tcatcccgaa gcggcgaggc gctgttgaga ttccatcttc tcgaccgtga agaagagctc
     1021 cgggtttggt taagacccgc acttaaggac tggatgcttg tcggactggc cgagggaaca
     1081 gttggataca ataccacgcg cggcagccgc agtaatctcg cggatgccgg cgtggatgat
     1141 aagacttata gtgagggacg cgctgcctgc ttcgcgcgcg gctccataca gggggatctc
     1201 ctgctgaccc tggcgcttga ttccagaaga tggaactcca atcccgacag taatatgttc
     1261 aggaccatca acccggaggc tttctatccc ctgtacggtg acaacatgat gcaggattac
     1321 ggttcatcga gttccagagg gatcttcgca aggcttgagg cggccaatta ttatgccatg
     1381 ttcggagatt acagcaccgg tctcaatgtc accgaattat cacgttacaa caggaccttc
     1441 aatggcctca aatttgaggg caatgcagaa aggaaaaatt ttaatctctt tttgagcagg
     1501 tcaaatcagg cctttgtaaa agatgaaatt cagggagacg gcacctccgg accctatgct
     1561 ctttcccgta acaatattgt cgtcaattcc gagcgggtgg ttattgaaac gcgcgaccgg
     1621 ttccagaatg atgtaataat ctccaggcag acgatgcggc agtttctgga ttacgatatt
     1681 gactattaca gcggcgtgat atttttcaag cggccggtac cgagccgcga tgagcagttt
     1741 aatccgctgt tcatcacagt agattacgaa tccaatgatg ccaatgaccg gtcatacaac
     1801 tatggcggcc gcgtcggaat agacttggcc gaagagagtt taaatgtcgg aggaacctac
     1861 atccatgaag ggcgaatcgg aggatacggt catctcggca gcattgacgc aacccttgcg
     1921 gttggggacg gcatccagct caagaccgag ttcgccgcct caaaagtgaa ggagggtacc
     1981 ggacgctcaa gcggaaatgc ctatctaatc gagcttgaac gcaaaatgaa aaaactcagt
     2041 ggcaagatat ttatccgcaa cctggacccc gaattcggtt tgggacagtt gagcctgagc
     2101 gaacgcggga tgcgcaaatt cggagcagct gccacgtaca gattgcgtga gtcgcttatt
     2161 ctgaacgggc atgcat
//
LOCUS       DSEB01000157            8291 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000455, whole genome shotgun sequence.
ACCESSION   DSEB01000157 DSEB01000000
VERSION     DSEB01000157.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8291)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8291)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8291
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000455"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="ENO07_02865"
     CDS             complement(<1..1284)
                     /locus_tag="ENO07_02865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HDS00938.1"
                     /translation="MHPWRIIICTSLIITILPGLLLSVDLSKGLFYDLPDENIAALVG
                     KEPIRQISSQDSAAIVNYRYDGDTAKVLAILVEWSTRPATYSPETMDSLMFSRNVFSG
                     GSVADYYNEVSYGQLAVVGDVTEWVDGGTYNSDYQFADLFATLDPVIDYSQYDGNNDG
                     DVDAVIFIRSGNGQEDSGDPDDIWSYAYSYPPGYGWGPFDGVMIPRWNTSPETWPLRN
                     PSNPTQFLGIDTLNRIRVFCHELGHNLGLPDLYDYDSKLVIQTYYTPNDANDHPLMDW
                     CTMGYGGYGIFSIGSMTPSHFCGWSKRELGWIEPIQLQNGTFENLVIYDIETHNDSAL
                     YVLPINASGSEYYLLEYRNSQSTGMFDKFDSDFSCFFYPLLSYGGDPLNSGLLITHVD
                     DSASPGAWQPNYGAPDYDHYTVAVVDAGYNIYNNYM"
     gene            1684..2079
                     /locus_tag="ENO07_02870"
     CDS             1684..2079
                     /locus_tag="ENO07_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00939.1"
                     /translation="MCLFFLFGLFFVGNGAVLCIGDDGHIEFETHCVPGCGETKEAPE
                     IDLSDALQVEHIDCSNCTDIDLNNPLWSRRGQTYLSGYTADFSLMSMPNADLRLTCTQ
                     ADIRMVNPEQLAFGHNPSSCSIISTILLC"
     gene            2172..3485
                     /locus_tag="ENO07_02875"
     CDS             2172..3485
                     /locus_tag="ENO07_02875"
                     /inference="COORDINATES: protein motif:HMM:PF02321.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDS00940.1"
                     /translation="MKGKIYTFLAYIQVIFLVISAHAGSEEFWEAPFDTASADIRISD
                     SAFSLNEVLKLVAKENPRFQSFDNQLEAARGSVKQAGLWSNPELDIEFEEVSWDAPGF
                     RESEFTVSLAQEFEFFGQRGARQEVAKAQLEAMELQVKMTAFDLYLITKQRFYALAHA
                     QQKVELLQRAVEVAEEVVENTEYRMDKGAALQSELILSRLNERRTRLALDQARQDVDA
                     LEATLVSLWMARPSGLRVFTEAEPDFTGAVEKLDLLSNRIDSSRQIVQMHNELKVIGA
                     ERARAIAGARPTITLSGGVKRLEADNSKTFLLGLSLPIPLFNRNQGMRESLDAQLRSL
                     EYDIERGKTEAYSKIHSNSIRLKMLIDKHAVLDSLLLPTAAEAYRMLQDAYEAGKVPY
                     TQLLEAERALNDLNFEHNDMLLAIQEQIMTLERLTGVVMNTEKEK"
     gene            3489..4568
                     /locus_tag="ENO07_02880"
     CDS             3489..4568
                     /locus_tag="ENO07_02880"
                     /inference="COORDINATES: protein motif:HMM:PF16576.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HDS00941.1"
                     /translation="MFGKFLITLTFLLLVWPLADASSQRNQGHVHNGEAGSEENSDFD
                     GHENPEHHDHESRADQKLSGEHTVNEHSNNEVHAGHSDELIIELSEAAVELASIEISE
                     VESGRIGKTIELPGEIGFNEDRLADISPRFAGIALQANYRVGDFVEEGNVVAIIESNE
                     SMNSYSIKAPISGWVIERHVTPGEFVSGENSIYVIADLSTVWINLAVYPKDADRIKKG
                     QLARIKAVGSGNVTSGAIEYVTPIIDLRTRSAVARITLPNPNNSWRPGTFVQAEVTTE
                     TGNQSLVVRKEAVQFLDEKTVIFVPDGPGRYRPVEVLTGETDTDYIQILSGLSEGIRY
                     VSDGAFELKAKIITSNLDAHAGHGH"
     gene            4583..7681
                     /locus_tag="ENO07_02885"
     CDS             4583..7681
                     /locus_tag="ENO07_02885"
                     /inference="COORDINATES: protein motif:HMM:PF00873.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDS00942.1"
                     /translation="MKIFFELILKNRLIILILLIGVIILGTFQYKSLPTDAFPDISPI
                     MVPVFAEAHGMAPEEIERLITFPIESTMNGLPGVTMVKSTSAFGMAVVYVYFEDDVDI
                     YFARQIVAERLAGASGELPDMNDPPALGPISTGLGEVFMYYLTADSTIETGGKPVNTY
                     LRELNDWVIKYQLQTVPGVTKILSMGGHVLQYQIEINPYALNKYELGLDDIVDAISNN
                     NRNAGGQFLVLGSEEYLVRGIGLLETRDDLRNIQLKVVSGIPVRLGDVAEVDFGREIR
                     RGVVTRNGEQEVVAGIVMKLFGTNTSDVIKNLNQRIPEVQGSLPQGVHLVPYYNQNAL
                     VNNAIHTVRNALFQGALLVIIVLALFLGNARSALIVALALPVCALISAIFMSWAEMSA
                     NLMSLGGIAIAIGMLGDASIVIVENIYRLLGDQRYENKSKFEIIVAACTEVARPIIFS
                     VSIIIIVFLPLFTLEGVEGKMFSPMAFTITFALLGSIIAALMFSPVLASLLLKKGHRK
                     EFFFLGKLKLLYRPFLMLVLSHRVTVVVTTLLIFAGTLLLLPFLGTEFIPTLEEGTIQ
                     INVTMAPSISLVKATETIMKLERIVMGFDGVEQTVSKIGRPEAGSHPHPVNSSHIQVM
                     LKPQDEWQRFHSKEELVEALNEKLSDYPGIQLVFSQPIQNMFDELLSGVKTQLAIKLY
                     GEDLKTLRSKAEEIKEVIEPVEGLVDLSTEQSFGQPQVQIVANRDACARYGVDISEIL
                     SVVELAVGGEVISQVFLNTRRFGIQVRFDERYRNKPESIRNILVHSSDGVLIPLSHVA
                     TIETVIGPIQINREKNQRRWVISGNVRGRDMGSVVGDIKKLVRENIELPYGYYLEYGG
                     QFENQERAMRRLSIIVPVTFLLIFLLLYMSLGNIRSATLIFINVPLALIGGVLGLFIT
                     GEYLSVPASVGFIALFGIAVQNGLVLVSYINHLRRKGLEIAEAVIKASMLRLRPVMMT
                     ALTTILGLVPLLLSGGMGSEVQRPLAVVVVFGLISSTFLTLVLIPALYKWFAPRLTTE
                     TD"
     gene            complement(7805..>8291)
                     /locus_tag="ENO07_02890"
     CDS             complement(7805..>8291)
                     /locus_tag="ENO07_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDS00943.1"
                     /translation="GGAAGAIIGNYMDKQAAEMERDLEGAKIERVGEGIKITFDSGIL
                     FEVNKSNLQPVAETNLTKLAEILNKYEDTEILIEGHTDSTGPEEYNMDLSIRRANSVG
                     NYLAGQEVNPGRFTLMGYGEKQPIASNETLEGRQQNRRVEIAIYANDKLKKAAQSQVE
                     G"
BASE COUNT         2065 a         1952 c         2209 g         2065 t
ORIGIN      
        1 catatagttg ttgtagatat tatatccggc atcgacaaca gccacagtgt aatggtcata
       61 gtcaggggct ccataattcg gctgccaggc gcccggtgac gcggaatcat cgacatgagt
      121 gataagcagg ccgctattca gtggatcccc gccataggaa agcaaaggat agaaaaagca
      181 gctaaaatcg gaatcgaatt tatcgaacat cccggtcgat tgagaattcc tgtattccag
      241 cagataatat tcgctgccgc ttgcattgat gggaagcaca tacagggcgc tgtcattatg
      301 ggtttcaata tcatatatga caagattctc gaaggtgccg ttctgaagct ggatcggctc
      361 gatccagccc aattcccgct tgctccatcc gcaaaagtga gaaggagtca ttgagccgat
      421 ggagaatatg ccataccctc cgtagcccat ggtacaccaa tccataaggg gatggtcgtt
      481 ggcatcattg ggtgtgtagt aggtttggat gacgagtttg ctgtcgtaat catagaggtc
      541 gggcaagccc agattatgcc ccaactcgtg acagaagacc ctgatgcggt ttaatgtgtc
      601 gatccccagg aattgggtgg gatttgacgg attgcgcaag ggccatgttt ccggggaggt
      661 attccacctg ggaatcataa cgccgtcaaa cggaccccat ccatagcccg gaggatagga
      721 gtaggcatac gaccagatat catcagggtc gccggaatcc tcctgtccat tccccgaacg
      781 gatgaagatc accgcatcta catcaccatc gttgtttcca tcgtactgag agtagtcgat
      841 gaccggatcg agagtggcaa acaggtctgc aaattgataa tccgagttgt aggttccgcc
      901 gtcgacccac tcggtaacgt ccccgaccac agccaactga ccataggaaa cttcattata
      961 atagtcggct accgaccctc cggaaaagac attcctggaa aacatcaagc tgtccatggt
     1021 ttccggggaa taggttgcgg gacgggtgct ccattccacc agtatagcca gaacctttgc
     1081 ggtatccccg tcatatcgat aattcactat ggcggcgctg tcctggctgg aaatctgcct
     1141 gatcggttct ttgccgacca atgcggcgat attttcatcg ggtagatcat aaaagagccc
     1201 cttcgataaa tcaacgctta gaagtaatcc cggcaggatt gtgatgatta gagatgtgca
     1261 aatgataatg cgccaggggt gcatggcatg ctcctttatt atgtgccttg ctaatagtca
     1321 aattcgcggg tatgctaact taaatatatt tgaaaaccgc ttcgggtcaa gcagattcta
     1381 tttgacggca agaattaatt aattgtggta caggtatttc cggcccccgc atctgtagaa
     1441 tggccgaggc atgccgttca aaggcgaccg catatattct tattggggtt ccggatattt
     1501 ttatgcagtc ttcccggaac ttcagagtgt ctgtcagtat tattacctga tgatcgcaat
     1561 atttctatcg gattggaacc gtttgaagag cccccagggg gaataagcgg ttgaccgtcg
     1621 ggtatagtat tattatattg acgcgttatg aaaatgcaaa gtcagaaaat cgcagcatgg
     1681 cctttgtgcc tgttttttct ttttggtttg ttctttgtgg gcaatggggc ggttctttgc
     1741 atcggtgatg atggacatat agaatttgag actcattgtg ttcccggctg cggggaaacc
     1801 aaagaagccc ccgaaattga tttatccgac gccctgcagg ttgagcatat cgattgctcc
     1861 aactgcactg acatagatct caataatcca ctgtggtcga gacggggcca gacttacctt
     1921 tccggttaca cggccgactt ctcgctgatg tccatgccga atgccgacct gaggctgacc
     1981 tgcacacagg ctgatatccg catggttaat cctgagcagc tggcatttgg tcacaacccg
     2041 tcttcctgtt caattatctc aactattctt ctctgttaaa ccttacttat ctcttctcaa
     2101 ttaaacagca ttcccaacga gcccatcggg tttgtgcgga ataatttaca aaactatctg
     2161 gagattcata gatgaaagga aagatatata cgttcctggc ttacattcag gtgattttct
     2221 tagtcatttc agcccatgcg gggtcggagg aattctggga agccccattc gacactgcct
     2281 cggccgatat cagaatatct gattccgcat tctcactgaa tgaggtcctg aaactcgtgg
     2341 ccaaggaaaa tccaagattt cagtctttcg acaatcagct cgaggccgct cgcggttcag
     2401 tgaagcaggc ggggctgtgg tcaaatccgg aattagacat agaattcgaa gaggtcagct
     2461 gggacgcccc gggattcagg gaatccgaat ttactgtaag ccttgctcaa gagttcgaat
     2521 ttttcgggca gcgcggcgcc aggcaggaag ttgcaaaagc ccaactggaa gcaatggagc
     2581 tgcaggtaaa gatgacggct tttgacctgt accttattac aaagcaacga ttctatgctc
     2641 ttgcgcatgc gcagcagaaa gtcgaacttt tacagcgagc tgttgaggtg gcggaagaag
     2701 tagtcgaaaa cacggagtac cggatggata aaggagctgc actccagtct gaacttattc
     2761 tgtcccggct caacgaacga cgaacgcggc tggctttgga ccaggccagg caggacgtgg
     2821 atgcgctgga ggcgaccctg gtttctctct ggatggcaag gccatcgggt ttacgagtat
     2881 ttactgaggc cgagccagac tttactggag cggttgagaa acttgatttg ctatcaaaca
     2941 gaatcgactc gtcccgccag attgtgcaga tgcataatga attaaaggta atcggggcag
     3001 aaagggcccg ggcaattgca ggggcacgcc ccaccataac attgagcgga ggagtaaaga
     3061 gactggaggc ggacaattcc aaaacctttc tcctgggctt atctttacca ataccgctct
     3121 tcaaccgaaa ccagggtatg cgcgaaagcc tggatgcgca gctgcgttca ctggagtacg
     3181 atattgaacg aggcaaaact gaagcgtatt caaagatcca ttccaattcg attcgcttga
     3241 agatgctcat agacaaacat gcggtcctgg actccctgct ccttcccacc gctgcagaag
     3301 cttaccgcat gctgcaggat gcgtatgagg ccgggaaagt gccttatacg cagttgctgg
     3361 aagcggaaag ggcactcaat gatttgaatt ttgaacataa tgatatgctc ctggcaatcc
     3421 aggaacagat tatgacactg gaacgcctca caggagttgt aatgaataca gagaaggaga
     3481 aataggctat gtttggcaaa tttctgataa cattgacttt cttgcttttg gtctggcccc
     3541 tggcagatgc ttcgtcacag aggaatcagg ggcatgtaca taacggcgaa gctggatcag
     3601 aagagaatag cgattttgat ggtcatgaga acccggagca ccacgatcat gaatcaagag
     3661 cagatcaaaa attatccggt gaacataccg ttaatgaaca ttcgaataac gaggttcatg
     3721 ccgggcacag tgatgaatta attatcgaac tatcagaggc cgctgtagag ctcgccagta
     3781 ttgagatctc agaagttgag agcggccgta taggcaagac tattgaattg ccgggagaaa
     3841 tcggattcaa tgaagaccgg ctggcagata tttccccccg ttttgcagga atcgctttgc
     3901 aggccaatta cagggtgggg gattttgttg aggaaggcaa tgtggtggcg ataatcgaaa
     3961 gtaacgagag tatgaattca tactctatta aggccccgat ttcgggctgg gtgatcgagc
     4021 ggcacgtaac tccaggcgag tttgtctccg gggaaaacag catatatgtc atagccgacc
     4081 tgtccactgt ttggatcaat ctggcagtct atccaaagga tgcagatcga atcaagaaag
     4141 gacagctggc caggatcaaa gcagtgggtt ctggaaatgt caccagcggt gcaatagagt
     4201 atgtcactcc catcatcgac ctgcgaacca ggagcgcggt cgccaggatc accctgccca
     4261 atccaaataa cagctggcgg ccagggacat ttgttcaggc tgaagtcaca accgagacgg
     4321 gcaatcagag ccttgtggtc aggaaagagg ccgttcaatt cctggatgag aagactgtga
     4381 ttttcgttcc tgacggcccc ggcaggtaca ggccggtgga agttctcacg ggtgagactg
     4441 acaccgacta catacaaata ttaagcggcc ttagcgaagg tatcaggtat gtatcggacg
     4501 gtgcgttcga gctgaaagcc aagatcataa ccagtaatct cgatgctcac gcaggacatg
     4561 gacactaaag gggacagagc cgatgaagat tttttttgaa ctgatactga aaaatcgctt
     4621 gatcatatta atactgctca tcggtgtgat aatccttggc acatttcaat acaagagcct
     4681 ccccaccgat gcgtttccgg acatatcgcc gataatggtg ccggtttttg cggaggccca
     4741 tggaatggcg ccggaagaga tcgagcggct tatcacattt ccgattgagt ccaccatgaa
     4801 cggcctgccc ggggtgacga tggtcaaatc cacctccgct ttcggcatgg ccgtcgtgta
     4861 tgtttacttc gaggatgacg ttgacattta cttcgccagg cagattgtgg cggaacgact
     4921 ggccggagcg tccggggaat tgccggatat gaatgatccg ccagcgctgg gaccgatatc
     4981 gaccggactc ggtgaagtat ttatgtatta cctgacagcc gacagcacga ttgaaaccgg
     5041 gggcaaaccg gtcaatacgt accttcgaga gctcaatgat tgggtgataa aatatcagct
     5101 tcagacagtc ccgggcgtga ccaagatatt gtcgatgggc gggcatgtcc tgcagtacca
     5161 gattgagata aatccctacg cgttgaacaa gtatgaactg gggctggatg atatcgtcga
     5221 tgcgatctcg aataacaatc gtaatgccgg gggacaattt ctggtgctgg ggtcagagga
     5281 atatttggtc agaggaatcg ggctgctcga gacaagggat gatctgcgca atatccagtt
     5341 gaaagtagtc agcgggatac cggttcggct gggagatgtg gccgaggtgg acttcggccg
     5401 ggaaattcga cgcggggtcg taacccggaa tggagagcag gaagtggtcg cgggcatcgt
     5461 gatgaaactg tttggcacca acacctcgga tgttatcaag aacctgaacc agagaatacc
     5521 ggaagtacag ggatcgcttc cgcagggggt gcatctcgtg ccctattaca accagaatgc
     5581 gcttgtaaac aatgctatcc ataccgtacg aaacgcatta tttcagggtg cgttgcttgt
     5641 aattattgta ctggctctat ttctgggaaa tgcgcgaagc gccctgattg tagccctggc
     5701 tcttccggtc tgcgccctga tatccgcaat cttcatgagc tgggccgaga tgtccgccaa
     5761 tctcatgtcg ctggggggaa tagcgattgc gataggaatg cttggcgatg catccatcgt
     5821 gatcgtggaa aatatatatc gcctcctggg agatcaacga tacgaaaaca agagcaagtt
     5881 tgaaataatc gtcgccgcct gcacggaggt ggccaggccg ataatattct ccgtttccat
     5941 tatcatcatc gtgtttctgc cgctcttcac tcttgagggt gtggagggca agatgttttc
     6001 accgatggcc ttcaccatca ccttcgcgct cctgggatcg ataattgcag ccttgatgtt
     6061 ctctccggtg ctggcgtcac tgttgctgaa aaagggacac aggaaagaat tcttctttct
     6121 agggaaatta aagcttcttt atcggccatt cctgatgctt gtactgagcc acagggtcac
     6181 cgttgtggtc acgaccctgt taatatttgc aggcacatta ctcttgctgc cctttctcgg
     6241 aacagagttc attccaactc tggaggaggg gacaattcag ataaatgtca ccatggcgcc
     6301 ttctatttcg ctggtcaagg ccaccgagac catcatgaaa ctcgagcgaa tcgtgatggg
     6361 cttcgacggt gtggagcaga cggtgtcaaa aataggccgg cccgaagccg gcagccatcc
     6421 tcatccggtg aattcatctc atatccaggt catgctgaaa ccccaggatg aatggcagcg
     6481 gttccattca aaagaagagc tggtggaagc cctgaatgag aagttgtcag actatcccgg
     6541 gatacagctg gtattttccc agcctatcca gaatatgttt gatgaactcc tgtccggggt
     6601 aaaaacccag ctggcgatca agctctatgg tgaggatctt aagaccctgc gatcgaaggc
     6661 ggaagagatc aaggaagtta tagagcctgt ggagggtctc gtggacctct caacggagca
     6721 gagctttgga caaccgcaag tacaaatcgt ggcgaatcgt gatgcctgtg cccgctatgg
     6781 agtagatatc tccgagatcc tgtccgtggt ggagctggcg gtgggcgggg aggttatctc
     6841 ccaggtattt ctaaataccc ggagattcgg aatccaagtc cgctttgatg aacgttaccg
     6901 caacaagccc gaatcgataa ggaatatcct ggtgcactca tccgacggcg tcctcattcc
     6961 tctctcccac gtagcgacga tagaaacagt tatcggaccg atccagatca accgcgagaa
     7021 gaatcagcgg cgctgggtga tctccggcaa tgttcgcggc cgggatatgg gaagtgtagt
     7081 tggcgatatc aagaagctgg ttcgggagaa tatcgagctg ccttacggtt attatctgga
     7141 gtatggcggc cagtttgaga atcaggagcg ggctatgagg cggctcagta ttatagtacc
     7201 ggtgacattt ctgctgatat ttctgttact gtatatgtct ttgggcaata tacgcagcgc
     7261 cacattaata tttatcaatg taccgctggc attgattgga ggcgtgctgg gcctgttcat
     7321 taccggcgag tatttgtcgg tacccgcttc agtaggattc atagcgcttt ttgggatagc
     7381 ggtacagaat ggcctggtcc tggtatctta tataaatcat ctgcgccgga agggattaga
     7441 gatagcagaa gctgttatta aagcctctat gctgaggctt cgcccggtaa tgatgaccgc
     7501 cctgaccacc atactggggc tggtaccgtt gctgctttct ggcggcatgg gttcagaagt
     7561 tcagaggccg ctggctgtgg tggttgtgtt cgggcttatc tcgtccacgt ttttgactct
     7621 ggttttgata ccggctctgt ataagtggtt tgctccgcgg ctgacgactg aaacagacta
     7681 aataagaggg ctggatattt ctccagccct catttctttc aaatcagagc gagcagtatt
     7741 tagtctcggg cttttataaa atcgggcgcg aaagatattc ctggttgcct gattccatga
     7801 ctgcctatcc ttcgacctga ctttgggctg ccttctttag cttgtcgttg gcataaatcg
     7861 caatctccac gcggcggttc tgctggcgcc cctctaaagt ctcgttagag gcgatcggct
     7921 gtttctctcc ataacccatt aacgtaaagc ggcccggatt gacctcctga cctgcaaggt
     7981 aattcccgac cgaatttgca cgacggatag agaggtccat gttatattct tccggccctg
     8041 tggaatcggt atgtccttcg atcaggatct cagtatcctc atatttattt aggatctcgg
     8101 ccagttttgt cagatttgtt tctgccacgg gctgaaggtt ggacttgttg acctcaaaca
     8161 gtattcctga atcgaaggtg attttgatac cttccccgac tctctcgatt ttggcgccct
     8221 cgagatcacg ctccatctcg gccgcctgct tatccatgta gttgccgata atcgcccccg
     8281 cagccccgcc g
//
LOCUS       DSEB01000158            8327 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000450, whole genome shotgun sequence.
ACCESSION   DSEB01000158 DSEB01000000
VERSION     DSEB01000158.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8327)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8327)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8327
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000450"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1719
                     /locus_tag="ENO07_02895"
     CDS             <1..1719
                     /locus_tag="ENO07_02895"
                     /inference="COORDINATES: protein motif:HMM:PF13205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00944.1"
                     /translation="GQCCNKGEIGPVNRLASLFLVYLFSSLILLSCAKIEFPPGGPPD
                     ETPPEVVSTSPEQRALGVATDTEIRIEFSEKIKKEDAARSLLIVPEPEKFPDLDWDGS
                     TLKIKFEEKLMEDRTYLITLKTQLSDLRNNKLEQPYRLAFSTGEVLDTGSIRGYTVQD
                     HRPFKSADLWAFPYDSGFSIFDRQPLYVTQSDDSGYYKFEYLADGKYLCFAVRDVASD
                     RTFDPQADQIGIPIMPAVIDSAHPEVRGLNFVLSSQDTAGLRVVSADYTTDLLIAVEV
                     SDLVLASSLSAEKFKVRGPENDSLIELAYTFALQDSSDLIMLVPEFTLAGGIYRLEAT
                     DIKSVKGKPLSQELDTASFEVDVFEDEQPPRLVRSRPSEGQTDFPHDSDFEFLFSEPI
                     IIEGADTSLIYLLTGDSSYLRNFWVQAEGTVFRAQSDDTLGTGAEYSLFLNLRSIKDR
                     SGKNSAGDTIMTYAFRTKDIEDYGSISGTVTGIGNMVNPLLYIASTDRKSRVAFALDD
                     SMRFAVEVPAGNYFFYGFDDLDSNGVHFSGDLQGLQYSEPYFFFDDTVSVRARFETEN
                     IRLEAK"
     gene            1817..4021
                     /locus_tag="ENO07_02900"
     CDS             1817..4021
                     /locus_tag="ENO07_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDS00945.1"
                     /translation="MQRRRMKNGRTEPNAKNGRKFLSRKYILIGVLGIFLVTSGILSA
                     YVIHVYNHELPAIDRLYNIEPSLVTRIYARDGSVIQKYFAEQRNLVGYDRIPQHMVDA
                     LLSAEDKNFYNHWGISSRDVFRAVLKNILHGFGSQGASTITQQLSRMLFLNRDVSLGR
                     KIKEALTAIKIERLYSKNEILEMYLNQYDFGNRSFGIFEAAKNYFNKDVEELTIEESA
                     LLAGLIQAPYGYSPIRYPEKALKRRNAILELMAENKKLTAEEADSLKQIPIELDLSNR
                     PETYGQYFSEYVRQYLVDTYGDSTVYSGGLSVYTTLNPYIQKVAEDAIKEQTDLIQNR
                     IDSTYTPDHPDFAEYSVKYYDEEGDSTAYRHRQIQAASIVLENATGDILAMVGGKDFG
                     DTKFNRAVQSLLQPGSAFKPFVYTAAIDNGYSPCMQMWDTPVVYTIPGAKEWRPHNFD
                     SKFDGKMSLREGFYRSRNLIAIKLLNMIQPQQAIFYARKMGITSHLSPDLSLAIGTSE
                     VRLIDLAQAYSVFPNGGVKVRPRFIYKVVDRYGNILEENPVPEKEEVLSPETAYIMVD
                     MMKSVMDEPQGTGHGARWRGFHREAGGKTGTSDNFADNWFSGYTPQITSTVWVGFDVK
                     LSIGINQTGAKNALPVWTKIMIAAHENIPGDTPEAHFQAPDGIRYAHVCYDSGELATD
                     KCVDVGREIFTRKTLPKTYCHIHPSSGVFTDQNKKDFNISTPDTTDDGIQNW"
     gene            4015..4899
                     /gene="lepB"
                     /locus_tag="ENO07_02905"
     CDS             4015..4899
                     /gene="lepB"
                     /locus_tag="ENO07_02905"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDS00946.1"
                     /translation="MVEKSDNISANRLIDTGPRKVWLAGVMGIVFPGMGQLYTGQTIL
                     ALLIIMLVVLSHVLMSVVSPDLPEKLVIFRYAVYILAIVQAIVIAHRQKEQYQARVTN
                     NWIFYMVVLMVGLILTSLPGQLMYKFYQVDVDCMQGTYDRNDVLYVSRVHYWLEEPKP
                     DEIVLYRNARFDGRLSIGRVWAGEKEIEIRNNAIFLDGYQILTEGIEPVEVPEMRLDQ
                     TDIVEREEVHTVTIPEDSYLIIGDNSYGWADAACTEIVPKQLIQGRIEYIYYDSPAED
                     DSVKEVSTKLLIYKLFLE"
     gene            5043..5128
                     /locus_tag="ENO07_02910"
     tRNA            5043..5128
                     /locus_tag="ENO07_02910"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:5077..5079,aa:Leu,seq:caa)
     gene            5356..7713
                     /locus_tag="ENO07_02915"
     CDS             5356..7713
                     /locus_tag="ENO07_02915"
                     /inference="COORDINATES: protein motif:HMM:PF01640.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00947.1"
                     /translation="MSNMPCPSRKNSYFGILTILTISFLIITFLTSSLSAETASKSEM
                     DLAAENWLSYMVYHRGAWAEDISPQIIQRDDITVEGRLVGRLYSVEPSGFIVVPALKE
                     LPPIKAYSETSDISITQEGGFMQMFRDVLSHKINGFIEEYGSIDAVQTDQINEHRGAW
                     DIYAVPSEDFMNSIAMEGKGALTEGEILLFPNEWHQGYPYNALCPLGDGGRCVVGCVA
                     TAASQIMWYWQWPPAGTGTKTYWWNGDGGINAQYLSADFSDEYDWANMPPDCSGGCTS
                     AQVEALSEIAYEVGVAHEMDYGVDGSGTWPNENVWTNYFRYDQSIQTAYRGSYTAQGW
                     FDLIASEIDAGRPMPYTIPTHAIVCDGWRISGAYNQIHMNYGWGGWNNAWYTVDNLYY
                     PGGPGSEWMFINVMPVQDADDDGYLNDEDNCPVDYNPGQEDMDSDGVGDVCDNCIDTY
                     NPDQSDVDLDGMGDVCDPDADGDGILNEDDNCWLVGNVGQDDNDSDGFGNACDNCPDT
                     PNPDQGDVNGDGIGDACDGEVHITGDEPPDGYYSYEYFYQLEGVGGIPPYYWSLVAGA
                     QIPYGCIFNGDKVGTITGTPNYASTFVFKVRMLDSGDPPLADTGIFVITIHSPDSVCF
                     DQDGDGYGDEGHSDNDCPPDNCPTAFNPEQLDTDSDGMGDICDPCPLDEENDADQDGV
                     CESDDNCPDTYNPDQVDSDEDGVGDACERMCGDSNGDQQCNVSDAVFIINYVFVDGDA
                     PDPMWTGDANCDSSVNVSDAVWIINYVFIGGNNPCDTNSDGVPDC"
     gene            complement(7798..8295)
                     /gene="sixA"
                     /locus_tag="ENO07_02920"
     CDS             complement(7798..8295)
                     /gene="sixA"
                     /locus_tag="ENO07_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HDS00948.1"
                     /translation="MVNQTKGTVMRFYIVRHAQAKSKEEDPERPLTQIGRQEIKQTGS
                     LAAEREMKNVTRIFHSGKTRAKETAQLLAEHTNPDIKPEAADGLLPQDDPKIWADKLK
                     SGEFGGDIAVVGHLPHVARLATNLLCKDADEDFFDPHGASIFCLEQDEDKKWLLKWMI
                     TPETV"
BASE COUNT         2077 a         1961 c         2203 g         2086 t
ORIGIN      
        1 ggacaatgtt gcaataaggg agaaattgga ccggtgaaca gactggccag cctttttctt
       61 gtttatctat tttcatcgct gatcttgctt tcatgtgcaa aaattgaatt tccgcctggc
      121 gggccgcccg acgagactcc gccggaagta gtttccacat cccccgaaca gcgtgcccta
      181 ggagtagcca ccgacactga aatacgaatc gagttctcgg agaaaataaa aaaagaggac
      241 gccgccagat cactgctgat agtgcccgag ccggaaaaat ttcccgatct ggactgggac
      301 ggcagcaccc tgaaaataaa attcgaagag aaactaatgg aagacaggac ctacctgatt
      361 accctgaaga cacagctttc cgacctccgc aacaacaagc tggaacagcc atacaggctg
      421 gctttctcca ccggcgaagt tctggatacg ggcagtatcc ggggatatac cgtacaggat
      481 cacaggcctt ttaaaagtgc cgacctgtgg gcgtttccat atgacagcgg cttcagcata
      541 ttcgaccgtc agccgctgta tgtcacgcag tccgatgact ccggctacta taaattcgaa
      601 tatcttgcag atggaaaata tctttgtttc gcagtcaggg atgtcgcctc ggatcgaact
      661 ttcgatccac aggctgacca gataggaatt cccataatgc ccgctgtaat cgattcggct
      721 catccggagg tgcggggcct gaatttcgtg ctttccagcc aggacacggc cggtttgagg
      781 gtcgtatctg ccgattatac aacggatttg cttattgcag ttgaagtatc ggatcttgtt
      841 ttggcctcca gcctctctgc cgagaaattt aaggttagag ggccggagaa tgattccctg
      901 attgaattgg cgtacacctt tgccttgcag gattcatccg atctaataat gctggtgccc
      961 gaattcaccc tggccggggg catttacagg ctggaagcga ccgatataaa gtccgtaaag
     1021 ggcaagcctt tatcccagga attggatacc gcctcttttg aggtggatgt attcgaagat
     1081 gagcagccgc ccaggctggt gagatcgaga ccgagcgagg ggcagactga tttcccgcat
     1141 gattccgatt ttgaattcct gttttcagaa ccgataataa tagagggtgc cgatacctcg
     1201 cttatatatc tgcttacagg cgactccagt tatttaagaa acttctgggt tcaggccgaa
     1261 ggtactgtat ttcgtgctca atctgacgat actctgggta cgggggcgga gtattcatta
     1321 tttctgaatc tgcgctctat taaagaccgg tccgggaaga acagcgccgg agataccatt
     1381 atgacttatg cattcaggac aaaagacatc gaggattacg gctcaatatc gggtactgta
     1441 acgggcatcg gaaatatggt aaatcctcta ctctatattg ccagcaccga tcgcaagtca
     1501 agagtcgctt ttgcgctgga tgattctatg cgctttgcgg tggaggtgcc ggcgggaaat
     1561 tactttttct atggttttga cgatcttgac agcaacgggg ttcacttttc aggagatttg
     1621 caggggctgc agtattctga gccgtacttc ttttttgacg acactgtctc cgtacgggcg
     1681 cgcttcgaga ctgaaaacat ccggctggaa gcaaaataaa tccggatcaa aacgaaatgt
     1741 aatccgttga attctatagt aattggaatt atattttttt gatgcgtaat tcattgaaca
     1801 ctgtataatc ttaagtatgc aaagacgaag aatgaaaaat ggccggactg agccgaacgc
     1861 aaaaaacggc agaaaattcc tgtcgcgcaa gtatattctt atcggcgtgt taggcatatt
     1921 tctggtcaca agcggaattc tttctgccta tgtaatccat gtttataacc atgaactgcc
     1981 ggcaatagac aggctctaca acattgagcc atcgctggtt accaggattt atgcgcgtga
     2041 cggatcggtc atccagaagt attttgcgga acaacgcaac ctggtaggtt atgaccgcat
     2101 tccgcagcat atggttgatg cgcttctttc cgctgaggat aagaattttt acaatcactg
     2161 gggcatatcg tcgcgggacg tttttcgtgc ggtcctgaag aatattctgc acggcttcgg
     2221 ttctcagggc gcctccacaa tcacgcagca actgtcacgc atgcttttct taaaccggga
     2281 cgtcagcctg gggaggaaaa tcaaggaagc cctgaccgct attaagatcg aacggctgta
     2341 ctccaagaac gagattctgg aaatgtatct caatcagtac gatttcggca atcgttcttt
     2401 cggtatattc gaggcggcca agaattattt caacaaggat gtcgaggaat taacgataga
     2461 ggaatcggcg cttctggccg gtttgatcca ggcgccgtat ggctattcgc ccatccgtta
     2521 ccctgaaaaa gctctaaaac ggcgcaatgc gattctggaa ttaatggccg aaaataagaa
     2581 gctcacggct gaggaggccg acagcctgaa gcagattccg atagagctgg atctctccaa
     2641 caggccggag acctacggcc agtatttttc agagtacgtg cgccagtacc tggtggacac
     2701 ttacggagat tcgacagtat attccggtgg gctttctgtc tataccacgc tgaacccata
     2761 tatccagaag gttgcggagg atgcgatcaa ggagcagacc gacctgatac agaaccgaat
     2821 cgactccacc tatactccgg accatccgga ttttgcggaa tactctgtta aatattatga
     2881 tgaggaaggg gattcaaccg catacaggca ccggcagatt caggccgcca gtatcgtgct
     2941 cgagaatgca accggcgaca tcctggccat ggtgggcgga aaggacttcg gcgataccaa
     3001 attcaaccgt gcggtgcagt cattgctgca gccggggtca gcgttcaagc cctttgtata
     3061 taccgccgca atcgacaatg gctattcccc ctgtatgcag atgtgggaca caccggtggt
     3121 ctataccata ccgggagcca aggaatggcg ccctcataat ttcgacagca aattcgacgg
     3181 caagatgagt ctgcgggagg gtttctatcg ttcccgcaac ctgatcgcaa ttaagctcct
     3241 gaatatgatt cagccccagc aggccatatt ctatgcccgg aagatgggta tcacctccca
     3301 tctctcgccc gacctgtccc tggcgatcgg gaccagcgag gtgcgtttga tcgacctggc
     3361 gcaggcctat tcggtcttcc cgaacggtgg tgtaaaggtc aggccgagat ttatttacaa
     3421 ggtggtcgac cgctacggca atatacttga ggagaatccg gtccctgaaa aagaagaagt
     3481 gctcagcccg gagaccgcat atatcatggt cgatatgatg aagtcggtga tggatgaacc
     3541 tcagggaaca ggacatgggg cacgctggcg cggattccat cgggaggccg ggggcaagac
     3601 tggaaccagc gataatttcg ccgataactg gttctcgggt tatactccgc agataacatc
     3661 aacggtctgg gtgggcttcg atgttaaact ctctatcgga ataaaccaga ccggcgcaaa
     3721 gaacgctttg ccggtctgga ccaaaatcat gattgcggcc cacgaaaata ttcccggaga
     3781 tacacccgag gcgcatttcc aggcaccgga tggtatcaga tacgcacatg tctgctatga
     3841 ttccggagag ctggctacag ataaatgtgt ggatgtcggg cgcgagatat tcaccaggaa
     3901 gacattgcca aaaacatatt gccacataca tccctcatca ggggtcttca cggatcagaa
     3961 taagaaggat ttcaatatca gtacgcccga tacaaccgat gacggcattc aaaattggta
     4021 gaaaaaagcg acaatatctc cgctaaccgt ctgatcgata ccggcccgcg caaggtctgg
     4081 ctggccgggg tgatggggat cgtttttccg ggcatgggac agctttacac cgggcagaca
     4141 atcctggccc tgttgataat aatgcttgta gtgctgtcgc atgtacttat gtctgtggtc
     4201 tcccccgatc ttcccgagaa gctggtgatc ttcaggtatg ccgtttatat cctggcgatt
     4261 gtgcaagcca ttgtaatcgc ccatcgccag aaagagcaat atcaggctag agtcaccaac
     4321 aactggattt tctatatggt agttttgatg gtcggtctga tactgaccag ccttccgggg
     4381 cagcttatgt ataaatttta tcaggtcgat gttgactgta tgcaggggac ttatgatcgt
     4441 aacgatgtgc tctatgtcag ccgtgtacat tactggctcg aggagccaaa gcctgatgag
     4501 atagtgctgt atcgcaacgc gcgctttgat ggccgtcttt cgatcggtcg tgtatgggcc
     4561 ggggagaagg agatcgagat caggaataat gctattttcc tggatggtta ccagattctg
     4621 accgagggga tcgagccggt ggaggtgccg gagatgcggc tggatcagac agatatcgtt
     4681 gaaagggaag aagtacatac agttacgatt ccggaggaca gttaccttat tataggggac
     4741 aattcttatg gctgggcgga tgccgcctgt accgaaattg ttccgaagca gctgattcag
     4801 ggtcgtatcg agtatatcta ttatgattct ccggctgagg atgacagcgt aaaagaggtc
     4861 agcacgaagt tattgatata taagttgttt ctggagtagg aaatctgcat aaattcgtag
     4921 cgggggtctt gagcggccgg aggcggattg tgatccctcg ctcataccgc agggacaggg
     4981 ttaatatcgt ataaaaaata gcttgaaatt taaatactgc ttgtatatat aggcttacga
     5041 atgccggagt ggtgaaactg gtagacgcgc tggactcaaa atccagtggg gttcaccccc
     5101 cgtgccggtt cgattccggc ctccggcatt tttttataaa ccatcaggca tggatttcct
     5161 ccagttcaat ctccaatgcc gataaaacca tattcggatg ttctgaacat atttcaaatc
     5221 gttgactaag atgttgcgtt atatggatgt atggaggcaa aacaatcctt gacaaaaatc
     5281 aactttgcgg tataattaaa ttggaatttt atacttcgta ccattaccga ccgcacaagc
     5341 tatggaggtg ctaaattgtc aaatatgccg tgccccagca gaaaaaattc atattttggt
     5401 atcctgacga tacttaccat ctcattcttg attatcactt ttttaacttc cagtctctca
     5461 gccgagacag cctccaaatc cgagatggat ctggccgctg aaaactggct cagttacatg
     5521 gtctatcatc gtggagcctg ggcggaggat ataagcccgc agattatcca gcgcgatgat
     5581 ataactgttg aaggccggct ggtgggacgt ctgtactcgg tcgagccgtc cggttttata
     5641 gtcgtgccgg cgctcaagga acttccaccg attaaagctt attccgagac ctctgatatc
     5701 tcgatcactc aggagggcgg attcatgcag atgtttcgcg atgtcctgag tcacaagatt
     5761 aacggtttca tagaagaata cggcagtatc gatgcggttc agacggatca gattaacgag
     5821 catcgcgggg cctgggatat ttatgccgtg ccttcagaag attttatgaa cagcatagcg
     5881 atggagggca aaggcgccct gaccgaaggc gaaatacttc tctttcccaa tgaatggcac
     5941 cagggctatc cctataatgc gctctgcccc ttaggtgacg gcggacgttg tgtggtcggt
     6001 tgtgtggcca ccgcggccag ccagataatg tggtactggc aatggccccc ggccgggacc
     6061 ggcaccaaga catactggtg gaacggagat ggcggtataa atgctcaata tttgtccgct
     6121 gacttctcag atgaatatga ttgggccaat atgcctcctg attgcagcgg cggatgtacc
     6181 agcgctcagg ttgaggctct ctcggagatt gcttatgaag tgggcgtggc acatgaaatg
     6241 gactatggcg tggatggttc aggaacctgg cccaacgaaa atgtctggac caattatttc
     6301 cgttatgacc agtcaattca gactgcctat cggggcagct atactgccca gggctggttc
     6361 gatctgatcg cctctgaaat agacgcgggc cgtccaatgc cctatactat tcccacgcat
     6421 gcgattgtct gcgacggctg gagaatctcc ggcgcctata accagatcca tatgaattac
     6481 ggctggggag gatggaataa tgcctggtac acggtcgaca atctttatta tcccggaggg
     6541 cccggttcgg agtggatgtt catcaatgtt atgccggtgc aggatgcgga tgatgatggt
     6601 tacctgaacg acgaggacaa ctgcccggtc gactataacc ctggacagga agatatggac
     6661 agtgacggcg taggggatgt ctgcgataac tgtattgaca catacaatcc agatcagagc
     6721 gatgtggatt tagacggcat gggtgatgta tgtgatcctg atgccgatgg tgatggtatc
     6781 ctcaacgaag acgataattg ctggctggtg ggtaatgtag gccaggatga caacgattcc
     6841 gacggtttcg gcaatgcctg cgataattgc cccgacactc ccaatccgga ccagggggat
     6901 gtcaatggtg atggaatcgg tgacgcctgc gacggcgagg tgcatataac cggagatgag
     6961 cctccggacg gctattacag ctatgagtat ttttatcaac tcgagggcgt gggcggaata
     7021 cctccatact attggagtct tgtcgccggc gctcagatac catacggctg tatattcaat
     7081 ggagacaaag tcggtacaat taccggcacg ccgaattatg cttcgacctt cgtgttcaaa
     7141 gtgcgaatgc tcgactccgg tgatccgccc ctggctgata cgggcatatt tgtaatcaca
     7201 atacattccc ccgattcggt ctgctttgat caggatgggg acggctacgg ggatgaaggt
     7261 cacagcgaca acgactgccc gccggacaac tgcccgaccg catttaatcc ggagcagctt
     7321 gacacggatt ctgacggcat gggtgatatc tgcgatccct gtcctttaga tgaagaaaat
     7381 gacgccgacc aggatggcgt ctgtgagagc gatgataatt gtcccgatac ctataatccc
     7441 gatcaggttg acagcgatga agacggtgtg ggggatgcct gcgagagaat gtgcggtgat
     7501 tccaacgggg atcaacagtg caatgtttcc gatgcggtat ttataattaa ctatgtcttt
     7561 gtcgacggcg atgcacccga tccaatgtgg acaggggacg caaattgcga ttcgtccgtg
     7621 aatgtcagcg atgcggtctg gatcataaat tatgtattca tcggcggcaa caatccctgc
     7681 gataccaata gcgatggtgt ccctgattgc tgattctctt gatatcagat atagataaaa
     7741 agcccgttcg gcatgagcag aacgggcttt aactttaatg gtggattatc caataactca
     7801 cacggtctca ggtgtgatca tccatttcaa gagccatttc ttgtcttcat cctgctccag
     7861 gcagaagatg gaggcgccgt gcggatcgaa gaaatcctcg tcggcgtctt tgcacagcag
     7921 attagtagcc aggcgggcca catgcggaag gtgtcccaca actgcaatat cccctccaaa
     7981 ttctccgctt ttgagcttat cggcccagat tttcggatca tcttgcggca gcaggccatc
     8041 cgcggcctcc ggttttatgt cgggattggt gtgctccgcc agaagttggg cggtttcttt
     8101 tgccctcgtt ttgccgctgt ggaatatcct ggtgacattc ttcatctccc tctcggcggc
     8161 aagcgagccg gtctgtttga tctcctgacg gcctatctga gtgagaggcc tttcaggatc
     8221 ttcttccttg gattttgcct gagcgtgcct tacaatgtaa aatctcatga ctgtcccctt
     8281 tgtttgatta accataagtt aattaagcat aatgctttat gttaata
//
LOCUS       DSEB01000159            4382 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001774, whole genome shotgun sequence.
ACCESSION   DSEB01000159 DSEB01000000
VERSION     DSEB01000159.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001774"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            99..1901
                     /locus_tag="ENO07_02925"
     CDS             99..1901
                     /locus_tag="ENO07_02925"
                     /inference="COORDINATES: protein motif:HMM:PF01935.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDS00949.1"
                     /translation="MINLGLHDNKTVTLDLATLTETRLMIQAMSGAGKSWLVRRILEQ
                     SYGRIQQLVIDLEGEFHTLREKFDYIIAGKGGDIPAELKSVKLLALKLLEFNASCIFD
                     LYELKKHERIAYVKAFSEALISAPRRYRHPVLVVLDEAHLICPEKGKAESASAVIDLA
                     TRGRKRGICTIMATQRLAKLNKDAAAELGNKMIGRTIQDIDRKRAVDELGFVSREESL
                     ALRKLQPGEFHLYGPAFRIHEGSQGYVPVNDIVLVKVGDVESSHPRIGTQHVETHPEA
                     TGKIKQLIEKLADIPHQAEKRAKDMIELKKELASLKRENTLLKKEVPVPCNHEPIIQA
                     QKSQIRHLQEELDSAAKKLEGQLQGIHLLSCNILKDIEKLTGLSLRPAELFEHIENLT
                     SGRGNEMHHIMQCETADCGAKPRTEKRAFPRPIRNSAMTQGSDLSNPQRRILEAIASF
                     EALGIDKPSKEMVAAKAQYSPKGGAFNNLLGRLRSNGHIDYPEAGKVELTESGRGVAG
                     EVEAIAGVEELHQGWLKIIKQGPLQKILEYLIQIYPESISREDLAKNLGYTEGGGAFN
                     NYMGRLHNTFEVIDYLPGRQVRAKQEVLFPEGLA"
     gene            1898..2386
                     /locus_tag="ENO07_02930"
     CDS             1898..2386
                     /locus_tag="ENO07_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00950.1"
                     /translation="MSEVLLVIGKPKYVGKGSGKSVRYHVRFLNRDVYLTPKSFSYLT
                     KLAYQSVVTSHWAFEDASGWVRKEQFDCGDCQARYMYNLKKEMGRDAFPDKYKIYPSK
                     PDLIVSDKNGRYKLNIGRPFLSIGYDNLLAFPDHEIRKIGAELKFEAERSCGGKEQNR
                     MD"
     gene            2358..3110
                     /locus_tag="ENO07_02935"
     CDS             2358..3110
                     /locus_tag="ENO07_02935"
                     /inference="COORDINATES: protein motif:HMM:PF07505.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HDS00951.1"
                     /translation="MAKSKIEWTDITINPIVGCTKVSPGCQNCYAEKMAKRLKAMGRP
                     QYQTVVDDRGWTGQTAFHKPALEQIRKAPVGKMIFIGSMGDIFHENVPEEWLTDIFRE
                     IGHNSKSVFQILTKRPERMAEIFPRMRYRFPDRLNHIWLGVTTENQEQADKRIPILMQ
                     IPAAKKFVSIEPMLGEIDLIPIVRDLDWVLVGCESGPRRRGCDPEWIDKILDQCRWGW
                     TPCFVKQVEINGKISHNMNEWPESIKRRELPE"
     gene            3443..3829
                     /locus_tag="ENO07_02940"
     CDS             3443..3829
                     /locus_tag="ENO07_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00952.1"
                     /translation="MAAVNSTAENMTRIYMAGAYSGSNILQIFRNMQIGIAESTEILK
                     AGMAPFCPWLDYQFFLHPGCNIDLETIRKYSIAWMEVSAAVKVVDNPANIDSKGLAEE
                     LEKARELGIPAFYRMDELKKWNSENI"
     gene            3829..4065
                     /locus_tag="ENO07_02945"
     CDS             3829..4065
                     /locus_tag="ENO07_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00953.1"
                     /translation="MPLFRPTAGYFKICLTWEKRDWESCKAFIKEIKANIEIEDRSYD
                     EESHEWEFKERCLPVVRDFWNKYFSDPNQQDMFE"
     gene            4075..4347
                     /locus_tag="ENO07_02950"
     CDS             4075..4347
                     /locus_tag="ENO07_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00954.1"
                     /translation="MAERFIREIKYFRSERLGEWVAVARPVGERRAVVETGLSEILAI
                     NKLKRLLREEGIDCPSDVEVRLAADHERMAFDQALAAGIGGLEGKF"
BASE COUNT         1268 a          911 c         1216 g          987 t
ORIGIN      
        1 attctgcagc tccccggttc gcggtatggc cccctttcac atatcgtcac cccatcccgg
       61 ggagctggtt aaatttaaca atctaacaat tggcaaagat gataaatctc ggattgcatg
      121 ataataaaac cgttaccctg gatctggcaa cattaaccga aacccggctt atgattcagg
      181 cgatgagtgg ggcagggaag tcctggctcg ttcggaggat cctggaacaa tcatatggca
      241 ggatccagca gctggtaatc gatctggaag gtgagtttca tacactcagg gagaaattcg
      301 attatatcat tgcgggcaag ggtggagata ttcccgccga actaaaatca gttaaacttc
      361 tggcattgaa gctcctcgag ttcaatgcat cctgcatatt tgatctgtac gaactcaaga
      421 agcatgagcg aatcgcgtat gtaaaagcat tttctgaggc cctaataagc gctcctcgga
      481 gataccggca tccggttttg gttgtgttgg atgaggcaca tttaatctgt cctgaaaagg
      541 gtaaagccga atcagcttcc gcggtgatag atctggcaac tcgagggcga aagcggggaa
      601 tttgtacaat aatggccaca cagagactgg ccaagctcaa caaagacgca gctgcggaac
      661 ttggcaataa gatgatcggc cgaacaattc aggatatcga tcgcaaaagg gcggttgatg
      721 agctgggatt tgtttcaagg gaagagagcc tggcgctccg gaagctacag cccggagaat
      781 ttcacttata tggtccggcc ttccgtatac atgagggttc acagggatat gttcccgtca
      841 acgatattgt cctggttaaa gtcggtgatg ttgaatcatc ccacccgagg atcggcacgc
      901 agcatgttga aacgcatcca gaggctactg gcaagatcaa gcaactgatt gagaagcttg
      961 cggatatccc tcatcaggca gagaagcgtg ccaaagatat gattgagctg aagaaggaac
     1021 tggcttcgct taaacgcgag aatacgctgc ttaaaaaaga ggtaccagtg ccatgtaatc
     1081 atgagccgat tattcaggct cagaaaagcc aaatcaggca tctacaggaa gaactggata
     1141 gtgcagctaa aaaacttgaa ggtcagttgc aggggataca tctattaagc tgcaatatcc
     1201 tcaaagatat tgaaaagctg accggattat ctctaaggcc tgcagagctt tttgagcata
     1261 tagaaaatct cacatccggt cgcgggaatg aaatgcacca tatcatgcag tgcgagacag
     1321 cggattgtgg cgccaaaccg agaactgaga aacgagcatt tccgaggccc atcaggaact
     1381 cagctatgac acaaggttct gatctatcta atcctcaacg gcgaatcctc gaggctatag
     1441 cctcattcga ggcgctcgga atagataagc ccagcaagga aatggttgcg gcaaaagctc
     1501 aatacagccc caagggcggg gcattcaata atcttctcgg caggctccgg tcgaatggcc
     1561 atattgatta tccggaagcc ggtaaagtag agctgaccga gtcaggaaga ggagtcgcgg
     1621 gagaggtcga agcgattgcc ggagttgagg agctgcatca ggggtggctt aagatcataa
     1681 agcaaggccc cctgcagaag atcctcgagt atctgattca aatctatccg gagagcataa
     1741 gcagggagga tctggccaaa aatcttggat acacagaagg cggcggggct ttcaacaact
     1801 acatggggcg gctgcacaat acattcgaag taatcgacta tctgcccggg cggcaagttc
     1861 gggccaaaca agaagtattg tttccggagg gattggcgtg agtgaagtct tacttgtcat
     1921 cggaaaaccc aaatatgttg gcaaaggcag tggcaaaagc gttcggtatc acgttcgttt
     1981 cttgaatcga gatgtctatc tgaccccaaa gagtttttcg tatttgacaa agctggctta
     2041 tcaatccgtg gtcacatcac attgggcttt tgaagatgcc agtggatggg tgaggaaaga
     2101 acagtttgat tgcggagatt gtcaggccag gtatatgtac aatcttaaga aggaaatggg
     2161 ccgcgacgca ttcccggaca aatataagat atatccaagt aaaccggacc tgattgtgtc
     2221 tgataaaaat ggccgttaca agctgaatat tggtcggcca tttctatcaa taggttatga
     2281 caacctgctc gcattcccgg accatgaaat caggaaaatt ggagctgaat taaagttcga
     2341 ggcagaaagg tcatgcggtg gcaaagagca aaatcgaatg gactgatata accataaatc
     2401 ccattgttgg ctgcaccaag gtgagcccgg gttgtcagaa ctgctatgca gagaagatgg
     2461 caaaacgatt gaaggcgatg gggaggccac aatatcaaac cgtggttgat gatcgcggtt
     2521 ggaccggcca gactgctttc cataaaccgg cacttgagca gatacgaaaa gctcctgtcg
     2581 gcaagatgat cttcattgga agcatgggag atatctttca tgagaatgtt cccgaggaat
     2641 ggctgacaga tatctttagg gagatcggcc acaattcgaa gtcagtattt caaatcctga
     2701 caaagaggcc tgaacgaatg gcggagatat ttcccagaat gcgctatcgc tttccggacc
     2761 ggcttaatca catatggctg ggagtgacca ctgagaatca ggaacaggcc gataagcgca
     2821 ttccgatatt aatgcagatt cctgctgcca agaagttcgt cagtatagag ccaatgctgg
     2881 gagagattga tttaattccg attgttcgag atttggattg ggtacttgtg ggttgcgaat
     2941 caggtccaag gcgccgggga tgcgatcctg aatggattga taaaatcctc gatcaatgcc
     3001 ggtggggctg gaccccatgc tttgtaaagc aggtcgaaat caacgggaag atcagtcata
     3061 acatgaacga atggccagaa tcaatcaaga gaagggaatt gccggaatga ccaccctggt
     3121 ccgcatcgtc gctgcagcgg tgtggctcgt tatcgccggc atttatgcgg tgctggcact
     3181 gcctatgctg gtcataattg gcgtgtgtaa gcaattaagt aagaggtaaa taaaagaaaa
     3241 ttggaattct atgcagggag gcaaatggca ggacatttga ggttgaagct aaccagaagg
     3301 gaagctcgaa cattggggga tgttcttacg gacatcaaca tccttaatat tatcgcaaaa
     3361 cggttcagcc tatccgcatc ggatatctgc aatcgggcgg gcatgaagat gggaaagcaa
     3421 ataaggagcg cggggaggca aggtggcagc cgtgaattcg acagctgaga atatgacgcg
     3481 gatatacatg gctggagcct actcaggcag caacatcttg cagatttttc gcaatatgca
     3541 aatcggaata gcggagtcta ccgagattct caaggccggc atggcaccgt tttgtccctg
     3601 gctggactat caattctttc tgcaccctgg gtgcaatatc gacctcgaga ccattcgcaa
     3661 atactcgata gcatggatgg aggtatcggc cgccgtaaag gttgtcgaca atcccgcaaa
     3721 tattgactcg aaggggctgg ccgaggaact ggagaaagct cgggagctgg ggatcccggc
     3781 cttttacagg atggatgaat tgaagaaatg gaattcggag aatatttaat gcccttattc
     3841 agaccgaccg ccgggtactt caagatatgt ttgacctggg agaagcgcga ctgggagagc
     3901 tgtaaggcat tcataaaaga aattaaggcc aatatcgaga tagaggatcg aagctacgat
     3961 gaggaaagcc atgaatggga attcaaggaa aggtgcctcc ctgtagtaag ggatttctgg
     4021 aataagtact tttcagatcc aaatcaacag gacatgttcg aatgaggagg ataaatggcg
     4081 gagagattca taagggagat caaatatttc aggtccgaga ggcttggtga atgggtggct
     4141 gtggcccggc cggtagggga aaggcgagct gtggtcgaaa ccggattatc cgagatcctg
     4201 gctattaaca agttaaagcg cttgttaaga gaagagggca tagactgtcc aagtgatgtg
     4261 gaggtaaggc tcgcggcaga tcatgaaagg atggcctttg atcaggccct ggcggccggc
     4321 atcggaggcc tggagggtaa attttgatcg acaaaatcca gaaatatctc aatgagcagg
     4381 ag
//
LOCUS       DSEB01000160            7745 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000514, whole genome shotgun sequence.
ACCESSION   DSEB01000160 DSEB01000000
VERSION     DSEB01000160.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7745)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7745)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7745
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000514"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1078
                     /locus_tag="ENO07_02955"
     CDS             <1..1078
                     /locus_tag="ENO07_02955"
                     /inference="COORDINATES: protein motif:HMM:PF00875.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribodipyrimidine photolyase"
                     /protein_id="HDS00955.1"
                     /translation="LLGEPEERLPDFLKKQKTGMLVTDFNPLKINRKWYRDIAERIDI
                     PFYQVDAHNIIPAWEISDKAEYAAHLFRKKVDEKLDDFLVEFPGLKKHKFGWDGRTPK
                     VNWGKARKSLKVDTKVGEVDGIRPGYTAGMDQFKEFINNRLTKYTDKRNDPTEDYQSG
                     LSPWLHFGQISAQRVVLETLKAAPGSKSQESFFTEVIIWRELADNYCFYNYNYDNIDG
                     FPDWAKKTLEEHRNDFREYMYSPEEFENAKTHDELWNAAQMEMVKTGKMHGYMRMYWA
                     KKILEWTPAPEQALDIAIYLNDKYELDGRDPNGYAGIAWSIGGVHDRPWFEREIFGKI
                     RFMSYNGCKKKFDVDKYIEKVKRM"
     gene            1546..1983
                     /locus_tag="ENO07_02960"
                     /pseudo
     CDS             1546..1983
                     /locus_tag="ENO07_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing stop; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-paratose 2-epimerase"
     gene            2059..3483
                     /locus_tag="ENO07_02965"
     CDS             2059..3483
                     /locus_tag="ENO07_02965"
                     /inference="COORDINATES: protein
                     motif:HMM:PF05368.11,HMM:PF11066.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDS00956.1"
                     /translation="MAKILVIGATGYVGRHLIPKLTQRGHHVRALVRDKARAPIDVWG
                     DDVELVEADVLKPETLPQALKDIEVVFYLVHSMSAGTDKFEKLDKKAAENTSRACKDA
                     GVHRIIYLGGLGSREDEKQSTHLRSRHEVGNILRESGILVTEFRAAILIGTESFSFEM
                     IHHLVNRLPVMVSPRWVQIKTQPIATGDALRYFVECLDKPESVGKIIDIGGPDVLSYY
                     DMMMMVARVLRLRRFILRVPVLTPRLSSYWIRLVTPLSVQMARSIIGSLKSETVVEDD
                     LAKQIFDFEPMAFEEAVRRALRGTQGAIVRTEAAGQFHPRGIQIDPSHLQIDQRNFEA
                     DVPAEKLYATVSRIGGDNGYFYADWLWRLRGWIDKMLGGIGMRKGRRHPTELEIGDPI
                     DFWRVEDLEPGKKILLRAEMNLWGQGWMEYLVEPLSPGRSRLTQTMRYYPRGLWGFLY
                     WKITYPMHMIIFKNMAKAIVAKAH"
     gene            4015..4572
                     /locus_tag="ENO07_02970"
     CDS             4015..4572
                     /locus_tag="ENO07_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00957.1"
                     /translation="MRRSTIRIIQILFLANLVAAMLVHDGINPGDAMWLVATGFCVML
                     AFVLFVDNRPHGLILRWAPLMKEMGFISAGNVRVKNELDDVYIFWKDESIGTGILFKR
                     DYSVDTVAFLNPPAVVNIMIKKIQMTKCEFYEIFFVERGRILIGDKDIIYWAYRGENA
                     EILYQLLMQNRYYAVRQPAPFPALQ"
     gene            complement(4581..4871)
                     /locus_tag="ENO07_02975"
     CDS             complement(4581..4871)
                     /locus_tag="ENO07_02975"
                     /inference="COORDINATES: protein motif:HMM:PF08803.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00958.1"
                     /translation="MVVQIVRFKSGLPEEEVIKTCEDRAPDYRAQDGLIQKYYLKYSD
                     TNEYGAVYIWDSQDALDAFNKSELRKTIPEAYQVQGAPDIGTAEVIMTLRSE"
     gene            complement(5365..6900)
                     /locus_tag="ENO07_02980"
     CDS             complement(5365..6900)
                     /locus_tag="ENO07_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDS00959.1"
                     /translation="MLAKILSAAVLGIDAYVVEVEADISQQLPAFATVGLPDGAVKEA
                     KERVAAAIKNSDFVFPAKKITINLAPADIRKEGSAFDLPIAVGILGATGQILRDDFEK
                     FIMVGELSLDGTLKPVPGILPIAIQIAKKKNYSMIVPKENAMEAAIATDLDVYPLGSL
                     RETVDFLEDGSGVKPCKIDVDQVFNQALKYPMDFSDVKGQETAKRALEVAAAGGHNIL
                     MVGPPGSGKTMLARRLPTVLPNLTMEEALEVTKIYSVSGLLPANTALIATRQFCSPHH
                     TISDAGLIGGGRVPKPGQVSLAHHGVLFLDEMPEFRKDILEMLRQPMEDGQVTISRSM
                     VSLTYPSQFMLVGAMNPCPCGFFGDTNHDCTCNPGQIQKYMSKVSGPLLDRIDIHIEV
                     PAVKFKDLAAEPTGEKSDSIRERVNHARKIQIMRFSDEPGMFCNAHMESKDLQKFCPL
                     DSKSLELLRTAITKLGLSARAYDRIIKVARTIADIEADEQIEIRHISEAIQYRSLDRN
                     LWM"
     gene            complement(6977..7669)
                     /locus_tag="ENO07_02985"
     CDS             complement(6977..7669)
                     /locus_tag="ENO07_02985"
                     /inference="COORDINATES: protein motif:HMM:PF13419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDS00960.1"
                     /translation="MSNEKIKAILIDVGGPLVDDSGLDIYWNDYLVKTLPDLIGRKVS
                     ISEIEEANRRSIESYAPSLYSATIWHFVQPDVEKFKKLRGAFDDLNTSQFYEFREEAG
                     EVCRQLSEKYSLAIAANQPASTADYLEEKGILKYFDFKEMSGSIAYSKPDYRFFLYIA
                     HRLDASPENCVMVGDRQDNDIVPAKKLGMKTVRVMVGSHKEQKVRMPSELPDKTISSI
                     SELSDAINDLQS"
BASE COUNT         2003 a         1869 c         1990 g         1883 t
ORIGIN      
        1 gcttctcggt gagccggagg aaagattgcc ggattttttg aaaaagcaaa aaacggggat
       61 gctggtgacc gacttcaatc cgctcaaaat caatcgcaaa tggtaccgcg atatagcgga
      121 gcgaatcgat atacctttct accaggtcga tgcccataat attataccgg cctgggagat
      181 ttcggataag gcggaatacg ccgcccattt gttcagaaaa aaagttgacg agaagctgga
      241 tgattttctg gtcgaattcc cgggtctaaa aaaacacaaa ttcggatggg atggcagaac
      301 tcccaaagta aactggggga aggccaggaa atcgctcaag gttgatacga aagtgggcga
      361 ggtggatggt atccggccgg gctataccgc cggtatggat cagtttaagg aattcattaa
      421 caaccgcttg acgaaataca ccgataagcg caacgatcca accgaggatt atcagtccgg
      481 cctttcgccc tggcttcatt tcgggcaaat ttccgcccag cgtgtggtgc tggaaacatt
      541 gaaagccgct cccggttcta aatcacagga gagctttttc accgaggtca ttatctggcg
      601 cgagctggcg gataattatt gtttctataa ctataattat gacaatattg acggttttcc
      661 tgactgggcg aagaaaacgc tggaggaaca tcgcaacgac tttcgcgaat atatgtactc
      721 accggaggag ttcgaaaatg ctaaaaccca tgacgagctc tggaacgccg cccagatgga
      781 aatggtcaag accggcaaga tgcacggcta catgcgcatg tactgggcca agaaaattct
      841 ggaatggact cctgccccgg aacaggcgct ggatattgca atttatttga atgataaata
      901 tgagctcgac gggcgcgatc ccaatggcta tgccggaatc gcatggagta tcggtggtgt
      961 gcacgaccgt ccctggttcg agcgtgagat tttcggcaag atacggttta tgagttataa
     1021 cggctgcaag aaaaaatttg atgttgacaa atatatagag aaagtgaaga ggatgtaatt
     1081 gaagttgtca ttccgagtga gtcccgccgg aggcgaggac gatcgaggat ccggctcctg
     1141 acggaccgtg ggaatctccg cctctgtctc agaaataacc gcggagatca tcacgtcgct
     1201 tcgctcctca tgatgacgag cattaggcat gtcattataa gggaacgaag tgaccgaaga
     1261 atctctcacg aatcaaacag attcttcgct acgctcagaa tgacaggata acccgtgtgc
     1321 agggtcattg accgcacaag gcgggatgtg accctgcggg cgaaaacagg ctgggtgtcc
     1381 cacatcttta gatgtgggag aaggatatta gaaacccacc cctgaagggg atgggccacc
     1441 ctgccacaga caaggatgat agattaaacg tacagcaggg tcctggcccc tacttgtcgg
     1501 gggtgtgacc ctgcggaaga gatcgaaaag atcgggggtg ataatatgaa aatacatact
     1561 ttaaatagag aacagctgat aaaaaagccg atcaatgagg tatttgagtt tttcgagaag
     1621 cccgaaaatc tggcgattat aactccgccg tcgcttggtt tcgaaataat aaccccgctt
     1681 ccgatcgaga tgagggttgg ggcattgata gactatacca taaaacttca gggcatacgg
     1741 gtgcgctgga caaccatgat caccgagtat gacccgccgc ataaatttct cgatgtacag
     1801 ctaaaagggc cgtacgcatt ctggcatcat acgcacagat ttaaggaagt cgagggcggg
     1861 actctgatgt ctgaccgtgt ccagtatatc ctgcccttcg gctttctggg aaggctggtg
     1921 catgcgctca gtatcgaggg ccggctaaag catatttttg attaccgtgc gaaagtcata
     1981 gaagagcagt tcgccagaga catcaaggat acgccgtcaa aggaagagct gaaaaagata
     2041 gcgaaggagt aaaggcgggt ggcaaaaatt ttagtgatag gtgcgaccgg ctatgtggga
     2101 cggcacctga ttccgaaact gacccagcgc gggcatcatg tccgggctct ggtgcgtgat
     2161 aaggccagag ctccgattga tgtctggggg gatgatgtcg agctggtcga ggccgatgtg
     2221 ctcaaaccgg aaacattacc ccaggccctg aaagatatcg aggtcgtatt ctacctcgta
     2281 cattccatgt ccgcgggaac ggataagttc gagaagctgg ataagaaagc ggccgagaat
     2341 acatcccggg catgcaagga tgcaggagta caccgaatta tctatctcgg cgggctggga
     2401 agccgcgagg atgaaaaaca gtcgacgcat ctacggagcc gtcacgaggt cggcaatatt
     2461 ctgcgcgaga gtggaattct ggtgactgaa tttagagccg ctattttgat cgggaccgaa
     2521 tcgttttcat ttgagatgat ccatcatctc gtcaaccgtc tgcctgtcat ggtcagtccc
     2581 aggtgggtac agatcaagac ccagccgatt gcgactggcg atgccctgcg ctattttgtg
     2641 gaatgcctgg ataagcccga gagcgttggc aaaatcatcg atatcggcgg gccggatgtg
     2701 ctctcatatt atgacatgat gatgatggta gccagggttc tcaggctgcg ccgctttata
     2761 cttcgtgtgc cggtgctcac tccgcggctg tcgtcatact ggattcgtct tgtcacgccg
     2821 ttatcggtgc agatggcgcg ttctattatt ggctcgctca agtccgagac tgtggttgag
     2881 gatgatctgg ccaaacagat ttttgatttt gagcctatgg cctttgagga ggctgtacgg
     2941 cgcgcactca ggggtaccca gggtgcgatc gtacgcaccg aggctgcggg acagttccat
     3001 ccgcgtggaa tccagattga cccctcacat cttcaaatcg accagcgcaa cttcgaagct
     3061 gatgttccgg ccgagaaact ttacgccacg gtctcgagga taggcggcga taacggctac
     3121 ttctatgccg actggctctg gcgcttgcgg ggctggatcg acaagatgct gggggggatc
     3181 ggcatgcgca aggggcggcg ccatccgaca gaactcgaaa tcggcgaccc gatcgacttc
     3241 tggcgtgtgg aggaccttga accgggcaaa aagatattac ttcgggcgga gatgaatctc
     3301 tggggacagg gctggatgga gtatctggta gagccgcttt cgcccggacg atcccgcctg
     3361 acacagacca tgcgctacta tccccgcggc ctgtggggct tcctctactg gaaaataacc
     3421 taccccatgc acatgattat cttcaagaac atggccaaag caatcgtcgc aaaagcgcat
     3481 tgagataatt ggccagcggc cggttcttca ggaattattg agaagctgcc aggaacgtat
     3541 cgcggggtct tgagcccaac gaagttggga ttgcgacccc gcgggaaggc atcagtgatg
     3601 gaagcgcact gaaataaccg tatagtaatt ccggacggcc aataccttct cacttgttga
     3661 aaaaccttgc aacagagaaa agaaccaggg cgcgtcattc tgagggagtc ccgaccctgg
     3721 tcgaggacga tcgaggatcc ggctcctgac ggaccgtgag aatctctaat ttttttgcat
     3781 caattaaatt ttgacatcat tactcgctct ggcccaaggg ctttcgctcc tcatgaagac
     3841 gagatcgaaa cttaaagtgc ttcaacactg ccttttagct gggattttca accttacatg
     3901 tacctcaaag aaaaaatgat ttggatgtgc tcagaaatag attattttta ttttgaggat
     3961 cgatccaggg caggcttttt ctgaaagctg cctgaattgg catggggaag gattatgagg
     4021 agaagcacaa ttcgaataat ccagattctc ttcctggcga atctggtagc tgcaatgctg
     4081 gttcatgacg gtattaatcc gggagacgca atgtggttgg tcgcaaccgg cttctgtgtt
     4141 atgctggcat tcgtattgtt tgtcgataac aggccgcatg gactcatctt gagatgggct
     4201 cccttgatga aagagatggg atttatttct gcaggaaatg tgagagtcaa aaatgaactt
     4261 gatgacgtct atatcttttg gaaggatgag tcgattggca cgggaattct gtttaaacgt
     4321 gattatagtg tggataccgt agcattcttg aatccgcccg cggtcgtcaa tattatgatt
     4381 aaaaagatcc agatgacaaa atgtgaattc tatgaaatat tttttgttga acgcggacgg
     4441 atcttaatag gggataaaga catcatctac tgggcatata gaggggagaa tgccgaaatt
     4501 ctctatcagc tcttgatgca aaaccgatat tatgccgttc gtcagcctgc acccttcccc
     4561 gcccttcaat aagtgcgcga ctattcagac ctgagggtca taatcacttc tgcggtgcca
     4621 atatcggggg caccctgaac ctggtatgct tcagggatag tcttgcgcag ctcagattta
     4681 ttgaaggcat ccagggcatc ctgcgaatcc cagatatata ccgcgccgta ctcgttcgtg
     4741 tcgctatact tcagataata tttttgtatg aggccatcct gagccctgta atccggcgca
     4801 cgatcctcgc atgtcttaat gacttcttcc tctggcaatc ctgacttgaa gcggactatt
     4861 tgcacaacca ttgtgtcacc tccttatccc cccgtgttgt ttgcatggaa gccacttcag
     4921 aattagctga ctttccatgc tattacttag tatgagagag gataacatat tataaggtgg
     4981 aaaagtgccg tcgttttgta tttttgtccc attatggtac ccgacatgaa atgtgggttt
     5041 gctcgatcat cctgctatgt ctgattttga agagcaacat gccaggtgaa attttcttcg
     5101 cccacctgtt aggtgggata caaagtgtct gatttagaaa gaaacatagc aggtgaaatt
     5161 ttcttcgtcc acctgttagg tggggtacaa atgttattat gtgtgcagaa tgtaatgaat
     5221 ctcgcacgtc catcatgatc ataatgtttt agtttcatga ttaaagacta tttgctatgc
     5281 tcgaagctcg tctttgctta tgaacatagc aggcaaaatg tttgaatcca cattgcatgt
     5341 ggggtaccaa taatgaattt ttcatcacat ccacaaattc cgatcaaggc tgcggtattg
     5401 aatcgcctcg gagatatgcc ggatttcgat ctgctcgtcg gcttcgatgt ctgcgattgt
     5461 acgcgcaacc ttgattatgc ggtcataggc gcgggcagaa agacccagct ttgtaatcgc
     5521 ggtacgcaac agctccagcg acttcgaatc aagcgggcag aatttctgca aatccttgga
     5581 ctccatatgg gcattacaga acatgcccgg ctcatcacta aagcgcataa tctgaatctt
     5641 gcgcgcatga ttgacccgct cgcgaatcga atcggacttc tccccggtcg gctctgcggc
     5701 aaggtccttg aatttaaccg ccggcacctc aatatggata tcgattctat ccagaagcgg
     5761 accggatact ttggacatat atttctgaat ctgcccggga ttgcatgtgc agtcgtggtt
     5821 ggtgtcgccg aaaaatccgc acgggcacgg gttcatcgcg cccaccagca taaactgcga
     5881 cggatatgtc aggctgacca tcgaacgtga aatcgtaacc tggccatcct ccatcggctg
     5941 ccgcagcatc tccagaatat ccttgcggaa ttccggcatc tcatccagga acaacacccc
     6001 gtgatgcgcc agcgaaacct gccctggctt gggtacacgt cccccgccga tcaatccggc
     6061 atctgagatt gtgtgatgcg gcgagcagaa ctgccgtgtg gcaatcaatg cggtgttagc
     6121 aggcagaagt cctgaaacag aataaatctt ggtaacctcc agcgcctcct ccatagtaag
     6181 gttgggaagt acagtcggca gtcttctcgc cagcatggtc ttaccggaac cgggaggccc
     6241 gaccatgagg atattatgtc cccccgcggc ggcgacctcg agcgcacgtt tggcggtctc
     6301 ctgccccttg acatcagaaa aatccatcgg atatttcaga gcctgattga agacctgatc
     6361 gacatcgatc ttgcacggct taactccgga accatcctcg agaaaatcaa cagtctcacg
     6421 cagcgatccc aggggatata catcgaggtc tgtagcaatc gccgcttcca tggcattttc
     6481 cttcggaact atcatggagt aatttttctt tttggcaatt tgaattgcaa tcggaagaat
     6541 ccccggcacc ggcttgagtg tgccgtcaag agaaagctct ccgaccatga tgaatttctc
     6601 gaaatcatca cgcagaatct ggccggtcgc gcccaggatg ccgaccgcta tcggcagatc
     6661 aaaagccgaa ccctccttgc ggatgtcagc cggcgcaaga ttaatcgtga tcttcttggc
     6721 cggaaaaacg aaatcggaat ttttgatggc cgcggccacc cgttccttgg cctctttaac
     6781 cgcgccatcg ggcagcccga cagtggcaaa tgccggaagc tgttgcgaga tatccgcctc
     6841 cacctccacc acataagcat cgatgcccag cacagccgca gaaagaattt ttgcgagcat
     6901 atggcaactc tcccgaaaaa gatgtgtcta tgtcacccta caatatggct ttaaaaaaat
     6961 aaaatactaa ttttcgctaa ctttgcaaat catttattgc atcagaaagc tcggaaatag
     7021 aagaaatcgt cttatcgggc agttctgacg gcatccttac tttctgctct ttatgcgatc
     7081 cgaccatgac ccgcaccgtt ttcatcccca gctttttggc cggcacaata tcattatcct
     7141 gtctgtcgcc taccatcaca cagttttccg gcgaagcatc aagcctgtga gcgatataga
     7201 ggaagaaacg gtaatccggt ttggagtaag cgatcgaacc ggacatctcc ttgaaatcaa
     7261 agtatttcag gatgcccttt tcctcgagat aatcagccgt ggaagcgggc tgattggcgg
     7321 caatcgccag cgaatatttc tccgacagct gccggcagac ctctcccgcc tcctctcgaa
     7381 actcgtagaa ctgactggta ttcagatcat caaaagcgcc cctcagcttc ttgaacttct
     7441 ccacatccgg ttgcacaaaa tgccagatag tggcagaata aagcgaggga gcataagact
     7501 cgatcgacct tcgattggcc tcctcgatct cactgatcga gactttgcgg ccgatcaaat
     7561 ccggcagagt cttaacaaga taatcgttcc agtaaatatc cagcccggaa tcatccacca
     7621 gcggcccgcc gacatctata agtatcgctt tgatcttctc gtttgacatg tgacagatta
     7681 tatgtaaaag ctctgattat gcaagcaaaa gataaaacga gaaatggttc gttactattc
     7741 aataa
//
LOCUS       DSEB01000161            1673 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1014974, whole genome shotgun sequence.
ACCESSION   DSEB01000161 DSEB01000000
VERSION     DSEB01000161.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1014974"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            814..1647
                     /locus_tag="ENO07_02990"
     CDS             814..1647
                     /locus_tag="ENO07_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00961.1"
                     /translation="MAFTNISIVKKHLAELRRPQKLVENFIFRLSGDEPLELPHKGIK
                     IGSEKIKGKEYNQPVYEAVTIADNPVSLGHAHLITDSVVAAADQSLTTIYRENIDYIV
                     DYRAGTISRIDGGGIQSGARISVWYYHFRLYQKDVDYAIDYASGKVTRLPGGELDAGQ
                     TIWVDYEIEAGIFSDEMISRSVEEAHTIVCGNIAEEYLESSDRLLEVAETYIALEILA
                     RMKGLEVMQSTFINPSRKSSIGKQYLQLGQSYRAEAENILVRYGAPSEALTYGIKIRN
                     N"
BASE COUNT          407 a          372 c          490 g          404 t
ORIGIN      
        1 ggaatatatt gatcgacagc gaggtcgctt ctgctccgat agtcaggcat aacggttcga
       61 cagcgtttgt attttactgc aagcaaatcg gccccaacca aaaccagctt aattatgccg
      121 tgaagaacgg ggcggagttc gcctcgccct caagcgtgtc gggctcgctg gcggcgtgcg
      181 accgggtgat ctgctatcgt cccgaagctt cagcaatgta ttatgacagg tcggttgagg
      241 cggcggatga cagcgctgca gatgtctatc acccggattc gggcggattg ttgaaggatt
      301 caggggatgc actctttctg ggccaattcg aaaaattcag ccggatttat attacacttt
      361 cgacgcccgg ctcagggggc acggtgacgt ggcactactg ggatggcgcc gactggaaga
      421 actttgttcc tgtttcgggc cagtataact ttgattcgtc tccggcacgg atacggttat
      481 gggaggattc gaatgatatc ccgtccgact ggcagtcgtc agtgatcgcc ggacattcca
      541 aattctggat caaggtagtt gtatcctctg attttgtcac tgctccggtg ggtacacaga
      601 taaccgcggc ggtagagttg cagtatatga cggcgcttta agatttctga tgagctgcgc
      661 tttcaatctt ataaggagga tgacatcatt cttattttca gaaggaagag atcgccaagt
      721 cgcctggtcc ggcaggagcc ggcccgcggc tcctcgcgat gacgaatcgt gtttacactt
      781 cggatttagg aatgtgtttt taaggaggag gggatggcat ttaccaatat cagtattgtg
      841 aagaagcatc tggctgaatt gagacgcccg cagaaactgg tggaaaattt tatttttcgc
      901 ttgagcgggg acgagccgtt ggagcttccg cataaaggaa tcaagattgg ttcagagaag
      961 atcaagggaa aggagtacaa tcagccggtg tatgaggccg ttactattgc ggataatccg
     1021 gtcagcctgg gccatgcaca tttgataaca gacagtgtgg tggcggccgc ggatcagtct
     1081 ttgaccacga tttatcgtga gaatattgat tacatagttg actacagggc cgggaccata
     1141 tcccgcatcg atggaggggg gatccagtcg ggggcccgaa tttcggtctg gtattaccat
     1201 ttcaggcttt accagaaaga tgtggattat gccatcgact acgcttccgg caaagtcacg
     1261 cgcctgcccg ggggcgagct cgatgcgggc cagactatct gggtggatta tgaaatcgag
     1321 gccgggatat tctcggatga aatgatctcg cgttctgtgg aggaggccca tacgattgta
     1381 tgcggaaaca tagcagagga ataccttgag agctcagaca ggcttctgga agtagcggag
     1441 acatatatcg ctctcgaaat actggcccgc atgaagggcc tggaagtgat gcaatcgaca
     1501 ttcatcaatc cctcccggaa gagttctatc ggtaagcaat acctgcagct ggggcagtca
     1561 tatcgagccg aggcggagaa tattctggtc aggtacggag caccatcgga agcgctgaca
     1621 tacgggatta agatcaggaa taactaatta cacgaaatga ggtagtctat gac
//
LOCUS       DSEB01000162            4384 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001773, whole genome shotgun sequence.
ACCESSION   DSEB01000162 DSEB01000000
VERSION     DSEB01000162.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001773"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..2029
                     /locus_tag="ENO07_02995"
     CDS             <1..2029
                     /locus_tag="ENO07_02995"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HDS00962.1"
                     /translation="NAGRPLGQLSACFVLPVGDSMEEIFETIKHAALIHKSGGGTGFA
                     FSRLRPRNSVVASTSGVASGPVSFMKVFNSATEAVKQGGTRRGANMGILRVDHPDIME
                     FISCKDDLSQVTNFNISVALTDEFMNAVEENREYDLIDPRTGKLYELDGKPQKLDARK
                     VFDEIVKHAWLTGEPGVIFIDSMNRKNPTNKCEKIEATNPCGEQPLPPYDSCNLASIN
                     LGSVVYEPLPGDYSPDEPEKGINWEKLLDLVKMGVNFLDNVIDMNKYPIPEIEQQTLS
                     NRRIGLGVMGWADMLVKLQIPYNSEKAFELGEKVMEFINSKGHIHSSEMAKTRGKFPK
                     WKDSIYADEGVSMRNATITTIAPTGTISIIAGCSSGIEPYFAISYNRNVMDNTNLIEV
                     NPLFEKIAKERGFYSTELMEKIAASNSLKEFDEIPEDVKRVFVTAADVTPEEHIRTQA
                     AFQKHCDSSVSKTINLPNEATEDDVRTAYWQAFKTGCKGVTIYRDGSRQNQVLSTGKT
                     PGEKKAAEQGDAEPLPPQKVMPKERPTTLDGFTDKIVTGYGNLYITINKFGNKPFEVF
                     AQIGKSGFSTMADTEAISRLISLALRSGVDVDDIIDQLVGIGGASPIYQEGEMVMSIP
                     DAIAKILKKHFGNGHHKIDRDVNLEACPDCGGRIQHESGCLVCPSCGFSRC"
     gene            2045..2653
                     /locus_tag="ENO07_03000"
     CDS             2045..2653
                     /locus_tag="ENO07_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00963.1"
                     /translation="MSRYQPPGRLSKVSGQDFMIQIQTEFAWHPNARITTSVVLDGVI
                     IHKIQKEWNGPLETDDEQELIRKYINRQHDEVEKIIRTNQEFILNYSKTQGKDNDFEN
                     IIGIEGVASAFLLSSDGLMTPFREEEIDVRMVQLFERLFELVEFLDHMTRWGSMHEAY
                     LVLKEDRIMIFRYRESFLIVVLEKDASPGETAKRVMTFLKAA"
     gene            2655..3380
                     /locus_tag="ENO07_03005"
     CDS             2655..3380
                     /locus_tag="ENO07_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00964.1"
                     /translation="MENLLEQIKGVVGVSGAMIYDRELDETRMLMPANFDEEIRDELG
                     AKFLDLLGFVDDPAKFRIKLSRGWLIIRLAGRYAILIVAKGDLNISTLDLVLKSIQVA
                     LDNKTYTLPEKKEVVFTEDSSFTLVRAINLMVEHFSDTISRFQLAQMLRQSKSDLLEQ
                     YPDLKHFTVEHNASVILIKGSESRLDSYSIEAVAAWMNQFKEYAKMETVMVGFNLKDV
                     TGEIRRDLDNLGFYRKFQQFAHS"
     gene            4071..>4384
                     /locus_tag="ENO07_03010"
     CDS             4071..>4384
                     /locus_tag="ENO07_03010"
                     /inference="COORDINATES: protein motif:HMM:PF00596.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDS00965.1"
                     /translation="MLNILYYLDISSLNGLLSGMEDELQLRQELVRIGKLLHSKGFVA
                     GTDGNISCRLDSDRILITPSGRPKGYLDPDELVVLDKAGSQLMGTGTPSSEFKMHLAI
                     YN"
BASE COUNT         1134 a         1079 c         1160 g         1011 t
ORIGIN      
        1 gaacgccgga cgtcccctgg gacagctttc ggcctgcttt gtactgcccg tgggcgattc
       61 gatggaagag atattcgaga caatcaaaca tgccgcctta atccacaaat ccggcggcgg
      121 taccggcttc gccttttcgc gcctgcgtcc gcgaaattcg gtagttgcct ccacctcggg
      181 ggtggcctcc gggccggttt catttatgaa ggtattcaac tcggccaccg aagcggttaa
      241 gcagggtgga acccgtcgcg gcgcaaatat gggcatactg agagtggatc atcccgatat
      301 catggagttt atctcctgca aggacgattt gagccaggta actaatttca atatctcggt
      361 cgcccttaca gatgagttta tgaatgcggt ggaggagaat cgggaatacg atctgatcga
      421 cccgcgtact ggaaaactgt atgagctgga cgggaagccg cagaagctgg acgcccgcaa
      481 ggtcttcgat gaaatcgtca agcatgcctg gctgactggt gagccggggg tgatcttcat
      541 cgatagtatg aaccggaaaa atcctaccaa taaatgcgag aaaatcgagg ccaccaatcc
      601 ctgcggtgaa cagcccctgc cgccctacga ttcctgcaac ctggcttcca tcaatctcgg
      661 ctcggtggtc tatgagcctc ttccgggcga ctattctccg gatgaaccgg agaagggcat
      721 aaactgggaa aagcttctgg acctggtcaa gatgggtgta aatttcctcg ataacgtcat
      781 cgacatgaac aaatacccta tccccgaaat tgaacagcag accctttcca accggcgaat
      841 cggcctgggt gtaatgggct gggcggatat gctggtcaaa ctccagatcc cgtataattc
      901 tgagaaggct ttcgagctgg gcgagaaagt gatggagttc atcaacagca agggccatat
      961 tcattcctcg gagatggcca agacccgcgg taaatttccg aaatggaaag attcaatcta
     1021 tgccgatgag ggtgtttcga tgcgaaacgc caccatcacg accatcgccc ccaccgggac
     1081 gatctcgatc atcgccggat gttcatccgg aatcgagccc tattttgcga tcagctataa
     1141 ccgcaatgtc atggataaca caaatttgat tgaggtaaat ccgcttttcg agaagatcgc
     1201 caaggagcgc ggattctatt ccaccgaatt gatggaaaaa attgccgcct cgaattctct
     1261 gaaagaattt gatgaaattc cggaggatgt gaaacgggtt tttgttaccg ccgccgatgt
     1321 tactccggag gaacatatcc gcactcaggc agccttccag aaacattgcg acagttcggt
     1381 ctcaaagacg attaacctgc ctaacgaggc cacggaagat gatgtccgta cagcctactg
     1441 gcaggccttc aagaccggat gtaagggtgt aaccatatat cgtgacggca gtcgtcaaaa
     1501 tcaggtgctt tccaccggca agacccccgg ggagaagaag gcggcagaac agggggatgc
     1561 agagcccctg cctccgcaga aggttatgcc caaggagagg ccgacaaccc tggacggttt
     1621 cacggataag atcgttaccg gttatggaaa tctctacata accatcaata aattcggtaa
     1681 caaacccttc gaagtcttcg cccagatcgg aaagtccggc ttctcgacca tggcggacac
     1741 cgaagcgata tcgagattaa tctctctggc gctaagatca ggcgtggatg ttgacgatat
     1801 aatagatcag ttggttggta tcggcggggc atccccgatt taccaggaag gtgagatggt
     1861 gatgtcgata cccgatgcca ttgcaaagat cttaaaaaag catttcggaa atggccatca
     1921 taagattgac agggatgtta atctggaggc ctgccccgat tgcggcgggc gcattcagca
     1981 tgaatcgggc tgtctggtat gtccctcatg cggcttttcc agatgctaaa atcgtcaatg
     2041 taaaatgagc agatatcaac cgcccggacg gctttccaaa gtctccgggc aggacttcat
     2101 gatccagatc cagaccgaat tcgcatggca tcccaatgcg cgtatcacca cctcggtcgt
     2161 gctggacggc gttatcatcc ataaaatcca gaaggaatgg aacgggccgc tcgagactga
     2221 tgatgagcag gagctaataa gaaaatacat caaccggcag catgacgagg ttgagaagat
     2281 aatcaggaca aaccaggaat tcatactgaa ttacagcaag acccagggca aggataatga
     2341 cttcgaaaat attatcggaa tcgagggggt ggcaagcgca ttcctgcttt cctcggatgg
     2401 gctgatgacg ccgtttcgcg aggaagagat tgatgtgcgc atggttcagt tgttcgagag
     2461 attgtttgag ctggttgaat tcctggatca tatgacccgc tggggcagta tgcacgaggc
     2521 ttacctggtc ttgaaggagg accgcatcat gattttcagg taccgggaga gttttctgat
     2581 tgtggtcctg gagaaggacg cctcgcccgg ggaaaccgca aagcgagtga tgacattttt
     2641 aaaggcggca tagcgtggag aatcttctgg agcaaataaa gggcgtcgtg ggggtttccg
     2701 gtgcgatgat ttacgaccgg gaactggatg aaacccgtat gcttatgccg gccaatttcg
     2761 atgaagagat cagggatgaa ctgggagcca aatttctcga cctgctgggc ttcgtcgatg
     2821 acccggctaa attcaggata aaactctcgc gcggatggct gattatcagg ttagccggac
     2881 gatatgccat cctgatagtg gccaagggag acctgaacat ctccacactt gatctggtcc
     2941 tgaagtcaat acaggtcgcg cttgacaata aaacttatac tctcccggaa aaaaaggaag
     3001 tggtatttac agaggattcc tcctttaccc tggtgagggc cataaacctg atggtggagc
     3061 atttctccga cactatttcc cgcttccagc tggcccagat gttgcggcag agtaaatctg
     3121 atctactgga acagtatccc gacctgaagc attttactgt ggagcataac gccagcgtaa
     3181 ttctgatcaa gggctccgaa tcccgcctgg attcatatag tatcgaggct gtggcggcct
     3241 ggatgaacca gttcaaggag tatgcaaaaa tggagacggt tatggttgga ttcaatctca
     3301 aggatgtaac cggtgagatc aggcgcgatt tagataacct cggcttctac cgcaaattcc
     3361 agcagtttgc ccactcttga ataatttaaa gccgcacccg tgagcgcggc ttcaaattta
     3421 tgagagagag ggatttgact tgaaaaaaac tcccaaacct atacggtaaa gggcttttat
     3481 agccgcctgg gcatttactc cccgctactc atcctgaggt ctcggcgtag gatccagcca
     3541 catgggggcg accttcatta tgtcaaattg atagagattt tttcaattgc cctctctcca
     3601 tctaacctaa tctacgcagg caacccttaa tatgcaaggt ttatttctgc gcagacctgt
     3661 aaattgttgt agctcaatcc ggtacaagtg atataaattt cggcgttgat tgagccgggt
     3721 ccgccgctgc gaagcgcaat attgaaaaag ggcttgaaag cggaagttat caaggggcga
     3781 gtcattctga gggagtcccg gccaccgccc gaggacgacc gaagaatctc atacaatcca
     3841 aggagattcc tcatcctgat aaatcggacc cgcaatggta gttttttaat tagtgcgttg
     3901 tgtacgtccc cgtgcacaac gctctgatgc ggcgcatgat gacatacgcc gcgcacgact
     3961 tgatgttgct gctttcaatc ggtgccccgc tgcgtaagcg atgggtttct tgatgatagc
     4021 agtcccaccc cttacggtcc gaaaatcggc ctgaccggga tggggtaccc atgctaaaca
     4081 tcctctatta tcttgacatt tcctctctaa acggcttact ttcgggtatg gaagacgaac
     4141 ttcaactcag gcaagagctt gtacgcattg gaaagctcct gcacagcaag ggtttcgtgg
     4201 ccgggaccga cggcaacata tcctgccggc ttgacagcga cagaatcctg ataaccccct
     4261 ccggacgtcc caagggatat ctcgatcccg atgagctggt ggttctggac aaagccggct
     4321 cccagttaat gggtaccggc acaccctcga gtgaattcaa gatgcatctg gccatatata
     4381 ataa
//
LOCUS       DSEB01000163            2233 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1007923, whole genome shotgun sequence.
ACCESSION   DSEB01000163 DSEB01000000
VERSION     DSEB01000163.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1007923"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            35..409
                     /locus_tag="ENO07_03015"
     CDS             35..409
                     /locus_tag="ENO07_03015"
                     /inference="COORDINATES: protein motif:HMM:PF13291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDS00966.1"
                     /translation="MVGFITRGRGVTVHRRDCPNIQRANIDKARIVELDWDISEQSKF
                     VVKINLIMEDRKNILRDILNSIADDETNLKNSSISASRGLARGEFIAEVRNLTHLNRI
                     LDQIKKVKGVIDVTRSMGSSKK"
     gene            479..1234
                     /locus_tag="ENO07_03020"
     CDS             479..1234
                     /locus_tag="ENO07_03020"
                     /inference="COORDINATES: protein motif:HMM:PF00156.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDS00967.1"
                     /translation="MKDNISKIRQSAEHIFDSIMGFVYPPQCVICDKLHEGERFFLCD
                     RCAHALDFYTAPHCFKCKNMIDSGGRKCSRCSGSDRISKIWACSAYDDFIRPLIHSFK
                     YAGVIPAGKYLAVKLGQLMADGLDAHDVDTIVPVPLHPMRERKRGFNQSIFISEWLQE
                     ELNISVYADALIRIRRTRDQTGLNRQKRRENMLGAFRANSKIDLEDKSVILVDDVTTS
                     GATASEAAKVLKNAGVSEIQLAVLAIAGHDLQE"
     gene            1300..>2233
                     /gene="xerD"
                     /locus_tag="ENO07_03025"
     CDS             1300..>2233
                     /gene="xerD"
                     /locus_tag="ENO07_03025"
                     /inference="COORDINATES: protein motif:HMM:PRK00283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDS00968.1"
                     /translation="MNKHDQNKRCLLDFLGYVDLELNLSHNTIAAYRHDLFDFCEFLR
                     QRSGRSLTVAGDAEILDYIINLRKKGISARSAARKLSAIKHLYKFLLAEKHVKDDPTT
                     SLESPKLLRALPDVLSKDEVTRLIESCDGEDPLGLRDRAILELWYACGLRISEVGKLK
                     IGDVVMEIDILRVKGKGNKERLVPFGSYAKKALEVYLRDSRPLLVKDKSFDTLFLSRK
                     GGGLSRMGLWGIFEKCVKKAQIMREITPHILRHSCATHMVEGGADIRTVMEFLGHADI
                     STTQIYTHLDQRYLKEVHRRFHPREKDYEPGNDLQ"
BASE COUNT          578 a          510 c          585 g          560 t
ORIGIN      
        1 tggcagatgc tgccagcctg taccgggaga ggatgtggtc ggattcatca cccgtggacg
       61 gggtgttacg gttcatcgca gggactgccc caatatccag cgtgccaata tcgataaggc
      121 caggattgtc gagctcgact gggatatcag cgagcagtcc aagtttgtcg tgaagatcaa
      181 cctcatcatg gaggatagaa agaatatcct gcgcgatatc ctgaactcca ttgcagatga
      241 cgagaccaat cttaagaaca gcagtatctc cgcttccagg gggcttgcca ggggtgaatt
      301 tattgccgag gtccgcaacc tgacacatct gaaccgcatc ctggatcaga tcaagaaggt
      361 caagggtgtg atcgatgtta cccgaagtat gggctcctcc aaaaaataaa cctgattaag
      421 cttgattatt tcggcattat ccataatttg aatccatatc catatctgtg aatttgctat
      481 gaaggataat atctctaaaa tccgtcaaag cgcggagcat atctttgaca gcattatggg
      541 tttcgtgtac cctccgcaat gtgtaatctg cgataaactt cacgaaggcg agaggttctt
      601 tttgtgcgac aggtgcgcgc atgcgctcga tttttatacc gctccgcact gcttcaaatg
      661 taaaaatatg attgacagcg ggggcaggaa atgttcacgc tgtagtggaa gcgaccgtat
      721 ttctaaaatc tgggcctgtt ccgcctatga cgattttatt cgccccctga tccattcctt
      781 caaatacgca ggggtcattc cggccggaaa atatcttgcc gtcaagctcg ggcaattgat
      841 ggctgatggc ctggatgctc acgatgtcga tacgattgtg ccggtcccgt tacatcccat
      901 gcgcgaaaga aaacgaggct tcaaccagag catttttata tctgagtggc tgcaggaaga
      961 gctgaatatt tccgtttatg ctgatgcgct cattcgaatc cggcgcacca gggatcagac
     1021 cggtttgaac cggcagaaaa gaagagagaa tatgcttggg gcattcaggg ccaactccaa
     1081 aatagacctg gaggacaaaa gcgtaatact ggtggatgat gttaccacct ccggcgcgac
     1141 ggcctcggag gcggccaaag tcctgaagaa tgcgggcgtt tctgaaattc aactggcggt
     1201 gctggccatt gccgggcatg acctgcagga ataaagtttt tgtttgattt caaatctgat
     1261 ttcctttcct ttgaaaagta gagctgagca tttaaaaata tgaataagca tgatcaaaat
     1321 aaacgctgcc ttctggattt tctgggttat gtggatctgg aactgaatct ctcccataat
     1381 acgattgccg cctatcgtca tgatctcttt gatttctgcg agttcctgag gcagcgatcc
     1441 gggcgctccc tgacagttgc gggtgatgcc gagattctgg actatatcat aaacctccgc
     1501 aagaaaggta tctccgctcg aagcgccgcc cgcaagcttt cggcaataaa acacctttac
     1561 aaatttcttc tggccgaaaa acatgtcaag gatgatccga ccacctctct ggaatcgcca
     1621 aaactcctgc gggctttgcc cgatgttttg tcaaaagatg aggtgacgcg cctgatcgaa
     1681 tcatgtgacg gcgaggaccc gctgggcttg cgtgacaggg caattctgga gttatggtat
     1741 gcctgcggtc tgcgaatttc ggaagtcggg aagttgaaga tcggtgatgt ggtcatggag
     1801 atcgatattt tgcgcgtcaa gggcaagggc aataaagaac gcctggtgcc gtttggaagt
     1861 tatgcgaaga aagccctgga agtctacctg cgtgattccc ggccgttgct ggtgaaagat
     1921 aagtcgttcg acaccttgtt cttaagccgg aaaggcggcg gcctgagccg catgggatta
     1981 tggggaatat tcgaaaaatg tgtcaaaaag gctcaaatca tgcgtgaaat cacgccccat
     2041 atactacgcc attcctgtgc tacacatatg gttgagggcg gcgcggacat tcggacggta
     2101 atggaatttc tcggtcatgc cgatatatct actacacaga tatatactca tttggaccag
     2161 aggtatttaa aggaagtgca caggcgcttt catccgcgtg agaaagatta tgagccaggg
     2221 aacgatttac aaa
//
LOCUS       DSEB01000164            3001 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004122, whole genome shotgun sequence.
ACCESSION   DSEB01000164 DSEB01000000
VERSION     DSEB01000164.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004122"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            41..>3001
                     /locus_tag="ENO07_03030"
     CDS             41..>3001
                     /locus_tag="ENO07_03030"
                     /inference="COORDINATES: protein motif:HMM:PF00082.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00969.1"
                     /translation="MNKRHHYQAFVCLLLIIFLPAESYSYLHEKAMDEKDIQTPSRLI
                     VKVKPLEVNSSGQGASDLNKVTGKTSLDKKYGITDTKLLFAGKSAGSANDLLSRIYIL
                     ECPRNTDLESAAREYEALEDIEYAHPDYLLELYLPPNDTHYGIQWPLNNVGQGYPHVL
                     RRDGDYNDTLITMYGTAGADINALEVFENPPDNTALVIIAVIDTGVDMDHPELAGRIW
                     QNPGEIPDNGIDDDHNGYVDDIRGWDYTSGFGAPTVPDNDPSDSYGHGTHCSGIIASL
                     TDNSSGIGGIVSDCKIMPLKFYPVMLSSYAAAAIVYAADNGADVISMSFGYPWQVEVL
                     EDALEYARSKGVVPCAATGNDGIAFSNYPGAYPSVISVGASTSDDEVAFFSTYGEQMD
                     VVAPGYSVLSLRAAGTDMYAPSEPNVHIIDENYYVASGTSMACPHAAAVAAYMMSASP
                     GLTPDAVQQIMQNTADDVLDPYGGGLNLPGWDQYSGHGRINLENALAAVPAITARITS
                     PLPNEILAGEIDVSGSAAGDDFTEYTLEYGIGSSPVSWTTITNSTSPVSDAVLGTFNT
                     AGLNGSFTIRLRVGEDNSDQVTVNVANDIVAYISAPLEDDTVISRVKIYGSATCPGFS
                     HYSLEYKAISGSVWNQFGQSAIPVKDDFLGEWKASALETANYHLRLSLYSDSGVVAQD
                     TVEVYLEHILAQEGNWKIEVANQISTVLNYGDFDNDAVNEIVVGTASGIKFYTPEGTE
                     KTDGMPQPPAYNFRIPPAVGDLNGDGLDDLVAVGKSGTAGKLYAHISNGQSFTTTLLA
                     VPNVSAYDQQSIQLFPVIFLKDIDSDDKDEIFFYEPPDCWIYDSDGSLIMRIPAPGLG
                     SFCQYLSNDSDGDGMDELYASHQTLYQFDADGVVLDSFDLTMDIAMNCDTRGISAVDI
                     DSDDKREIIVYVAMSDTQETYWVYAFDEGLALKDGWPHNTGINTFLVPPAPIFGDLDR
                     DGAPDYFSAFYELVH"
BASE COUNT          768 a          724 c          763 g          746 t
ORIGIN      
        1 attggtaatt gaaccgctaa actgcatcaa aggattagat atgaataaga gacatcatta
       61 tcaggctttt gtatgtctgc tgctcataat attccttcct gcggaaagct actcatacct
      121 gcacgagaag gcaatggatg agaaagacat acagacacct tcaagattaa tcgtgaaggt
      181 aaaaccgctt gaggtcaaca gctcgggtca aggggcgagt gatttaaaca aagttacggg
      241 caaaacaagt ctcgataaaa aatatggaat cacggacacc aaactgcttt ttgcgggcaa
      301 gtccgcaggt tcagcaaacg atctactttc gaggatttat atactggaat gtcctcgaaa
      361 cacggatctc gagtctgccg ccagagaata cgaagcgctg gaagacatcg aatatgcgca
      421 tccggattat ttgctggagc tttacctgcc tccgaacgac acgcattatg gtatccagtg
      481 gccgctcaac aatgtcggcc agggctatcc gcatgtgctg cgcagggacg gcgactacaa
      541 tgacacgttg attacaatgt atggcaccgc tggagctgac ataaatgcac tggaagtatt
      601 cgagaatccc cctgataata ctgctctggt aatcattgcg gtgatcgata caggggttga
      661 tatggaccat cctgagctgg ccggaagaat ctggcagaat ccaggagaga tccccgataa
      721 cggtattgat gatgaccaca atggctatgt cgacgatatc cggggatggg actataccag
      781 cggcttcggc gctccgactg ttccggataa tgatccatcc gactcgtacg ggcacggcac
      841 acactgctcc ggaataatag ccagcctgac cgataactcg tctggaattg gcggaattgt
      901 ctccgattgt aaaataatgc ccctgaagtt ttatcctgtt atgctgtctt catacgctgc
      961 cgcagctatt gtttatgccg ctgataatgg cgcagatgta atcagcatga gtttcggcta
     1021 tccctggcag gttgaggttc tggaagatgc cctggaatat gcccgctcta agggtgtggt
     1081 tccgtgtgca gccacaggaa atgatgggat tgccttctct aactaccccg gagcatatcc
     1141 atctgtcatt tcagtgggcg cctcaaccag tgatgacgag gttgctttct tttccaccta
     1201 tggagagcag atggatgtcg tagctcccgg ttactctgta ctatccctgc gggctgcagg
     1261 aactgatatg tatgcaccgt ctgaaccgaa tgtccatata attgatgaaa attattatgt
     1321 cgcctcgggg accagcatgg cttgtcccca cgccgccgcg gtggcagcgt atatgatgtc
     1381 cgcttcaccc gggttaacgc cggacgccgt tcagcaaatc atgcagaata ctgccgacga
     1441 tgtcctcgac ccctatggcg gtggactaaa tcttcccgga tgggatcagt acagcgggca
     1501 cggcaggatt aatcttgaaa atgcgctggc agccgtgccg gcaataacag cccgcatcac
     1561 ctcccccctg cctaacgaga ttctggcagg tgaaattgat gtaagcggca gcgctgctgg
     1621 tgatgatttt acagaataca ctctggaata tgggatcggc agcagcccgg tatcctggac
     1681 aactatcact aattctacca gccccgtaag cgacgccgtg ctgggaacat tcaatactgc
     1741 cggcttaaac ggaagcttta cgataaggct gcgtgtggga gaagataatt ctgaccaggt
     1801 gaccgttaat gtagccaatg atatagtagc atatatttcc gcgccactgg aagatgatac
     1861 ggttatatcc cgggtaaaaa tctatggctc ggctacctgc cccggcttca gccactattc
     1921 acttgaatat aaagcaatct ccggctctgt ctggaatcaa tttgggcaat ctgcaattcc
     1981 ggtcaaagat gattttcttg gtgaatggaa ggccagcgct cttgagacag cgaactatca
     2041 tctaagattg agtctctatt cagactcggg agttgtggcc caggatacag tggaagtata
     2101 tttagaacat attctggccc aagaaggtaa ctggaaaatc gaggttgcga accaaatatc
     2161 gactgtgctt aattatggcg atttcgataa cgacgctgta aacgagatag tggttggcac
     2221 tgcctcggga atcaaattct atactccgga aggaaccgaa aaaacggatg gtatgcctca
     2281 gccgccggcc tataacttcc ggataccgcc ggctgtggga gatcttaacg gcgatggtct
     2341 ggacgatctc gtcgcggttg gcaaatccgg gactgcggga aagttatacg cacatatctc
     2401 taatggacag tctttcacga caacgttgct tgctgttccg aatgtcagcg catatgatca
     2461 acaatcgatt cagctttttc ctgtaatatt tctaaaggat atcgacagcg atgataagga
     2521 cgagatcttc ttctacgagc cgcctgactg ctggatttat gattctgacg gctctctcat
     2581 aatgcggatt cccgctccag gattagggtc cttttgccaa tacctgtcta acgacagcga
     2641 tggggatggt atggacgaac tctatgcttc acatcaaacc ctctaccaat ttgatgcgga
     2701 tggcgtcgtg ttggatagct ttgacctcac catggatata gccatgaatt gcgatacgcg
     2761 cgggataagt gcggtagata tcgactccga cgacaagcgg gagatcatag tctatgttgc
     2821 catgtcggac acacaggaaa cctattgggt ttatgctttc gatgagggcc ttgctctgaa
     2881 agacggttgg ccgcataaca ccggcattaa tacctttctt gttccgcccg caccgatctt
     2941 cggtgacctg gaccgggatg gagcacccga ctacttcagc gctttctatg aacttgtgca
     3001 c
//
LOCUS       DSEB01000165            2829 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004688, whole genome shotgun sequence.
ACCESSION   DSEB01000165 DSEB01000000
VERSION     DSEB01000165.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004688"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1900)
                     /locus_tag="ENO07_03035"
     CDS             complement(<1..1900)
                     /locus_tag="ENO07_03035"
                     /inference="COORDINATES: protein
                     motif:HMM:PF01738.16,HMM:PF02897.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDS00970.1"
                     /translation="MNNIKSISTTVLILILISILIIGCIERREKEGESVAATEKRLND
                     FFEGDYIPDIERFMQIGYVTNIAISKDGRRVFLSPSYTNAPQVFRVTDGGWPYQLTSF
                     EDGLSWYRLSNDSYYAIVGAAVGGDENAQLYLLDTESGRIKKLIDRPEARFGGVVWSK
                     DDDFIYFSSNMGNLKDFFIYKLELPDREAVLVKEKEGWNVPIQVNEDGRYLLYEAAVS
                     SRDNNFYLLDLETGTQEILTPHEGEYLYPFMKLTPDFKTAYLTTNNTPDGITRIAEMS
                     VMTNEIVFLNSNQQWECESAALSDDGHYLFWTINEEGYDRPHFKDLVTGDMLPVPGMN
                     GLYSFGGFTADGRFLFSFHNANHAPNAWIWNPYEEMGARVTNVSTMGIDISEFVEPRL
                     IKYESFDSLEIPAFLYLPSNYTDGPIPFIMDFHGGPEGQFRPSFNRHFNYLLANGFGI
                     MAPNVRGSEGYGKEYLDMDNYKNRLKSVADGAAGARWLIDNGYTTSDMLGVKGGSYGG
                     YMVMALITEYPDLFAAAMNYIGIVNFVTFLENTAEYRREFRESEYGPLSDREFLRSIS
                     PIHKVDKIECPLLVIHGENDPRVPVGEARQIIRELKSRGGEVDSLIFPDEGHGASKLE
                     NRLEMYRTM"
     gene            2249..>2829
                     /gene="prs"
                     /locus_tag="ENO07_03040"
     CDS             2249..>2829
                     /gene="prs"
                     /locus_tag="ENO07_03040"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDS00971.1"
                     /translation="MPDQIKLLTGTANLPLAERIANYIGIPLTQREIKHFSDGEIFVK
                     IDENVRGTDVFIIQPTNPPAENIMELLIMVEACRRASASRITAVIPYYGYARQDRKSQ
                     PRVAITAKLLANLITAAGADRVVTMDLHVSQIQGFFDIPSDHLYASNLFYNHFRMRGM
                     TDIVVVTPDVGSIKIARAFANSLQCGLAIIDKRR"
BASE COUNT          695 a          698 c          704 g          732 t
ORIGIN      
        1 ccatggtacg gtacatttcc aaacgattct ccaatttgct ggctccatgg ccctcgtccg
       61 gaaagataag tgaatccact tccccgcccc gggacttgag ctcgcgtatt atctggcggg
      121 cctcgccaac aggtacccgc ggatcatttt caccatgaat aaccaggagc ggacattcaa
      181 ttttgtcgac cttgtgaatg gggctgattg atctgagaaa ttccctgtcg gaaaggggtc
      241 catattcgga ttcacgaaat tcgcggcggt attcggccgt attttccagg aatgtcacga
      301 agtttacgat gccgatataa ttcatggcag cggcgaagag gtcgggatac tcggttatga
      361 gagccattac catataacct ccgtaggatc cgcccttgac ccccagcata tcggatgtcg
      421 tatagccgtt atcgatcagc cacctagcac cggccgcgcc gtcagccacg gatttcaggc
      481 ggtttttgta attgtccata tccaggtatt ctttgccata accttcagag ccgcgcacat
      541 tgggcgccat aattccgaag ccgttggcca gaaggtaatt gaaatgacgg ttgaacgagg
      601 gcctgaactg cccttccgga ccgccgtgga aatccatgat aaacggtatc gggccatcgg
      661 tataattgct gggaagatat agaaatgccg ggatttccag gctgtcgaag gattcgtatt
      721 tgatcagccg gggttcgaca aactccgata tgtcaatccc cattgtggag acattggtga
      781 ctcttgcgcc catctcctca tatgggttcc agatccaggc attgggggca tggttggcgt
      841 tatggaacga aaacaggaag cggccgtcag ccgtgaaacc gccaaatgaa tacagaccgt
      901 tcatgcccgg aacaggaagc atatcacctg tcaccaagtc cttgaaatga ggcctgtcgt
      961 acccctcttc gttgattgtc cagaaaagat aatggccgtc atctgagagg gccgccgatt
     1021 cgcattccca ttgctggttc gaattaagga acacgatttc gttcgtcatc acactcatct
     1081 cggctatacg tgtaatgcca tcgggggtgt tattggtggt caggtaggca gttttgaaat
     1141 cgggtgtgag tttcataaag ggatacaggt attcgccttc atggggagtc aaaatttcct
     1201 gggtgccggt ctctaaatca agcaggtaaa aattgttgtc gcggctgctg actgccgcct
     1261 cgtagaggag atacctgccg tcttcgttaa cctgaatcgg cacattccag ccctccttct
     1321 ccttgaccag caccgcttct ctatcgggaa gttcgagctt atagatgaaa aaatccttga
     1381 gatttcccat attggaggag aagtaaatga aatcgtcatc tttggaccag accacaccgc
     1441 cgaatcgggc ctcgggacga tctatcagct tcttgatccg cccgctttcg gtatcgagaa
     1501 gatagagctg tgcgttttca tccccgccta cagcggctcc cacaatagca taataagaat
     1561 cattcgaaag tctataccag cttaggccgt cctcaaatga ggtaagctgg tagggccagc
     1621 cgccatcggt gaccctgaaa acctgagggg cattcgtata tgacggggac aggaacacgc
     1681 gccgaccgtc tttggagatc gcaatatttg tgacatagcc gatctgcata aatctctcga
     1741 tgtccggaat atagtcgccc tcgaaaaagt cgttcagacg cttttcggtt gccgctacac
     1801 tctcgccttc cttttccctg cgttcgatgc atccgatgat caaaatcgaa atcagaatga
     1861 gaataagcac tgtggttgaa attgacttga tattgttcat tatgtcctct ccgaatcttt
     1921 acggactatc tgcaatagcc gctcaatcct gtatcaggcc gaaacgaagc ctttcctgca
     1981 tattagcttc aggacttttt ccgggcctgt tttcattaaa tttatatcct tcggaaatct
     2041 tattcgcaaa gcaggaaaat aacaatattt taaggctgaa tcaaatcaaa tttccgcttg
     2101 acaatatgcc cgcggggata tactaagaag gttgtttctg agatggtttg agatggcagt
     2161 atatgcagtt ttccattgca ttttggggaa tgttgtatat attaggaaat tgtttttttg
     2221 ggggatttgg gaggtaaagg aataaggtat gccggaccag attaaattat tgacaggaac
     2281 tgcaaatctg cctctggcag agcggattgc aaattacatc ggtattccgt tgacccagcg
     2341 ggagatcaag catttttcgg atggtgagat tttcgttaag atcgatgaaa acgtcagggg
     2401 aaccgatgtt ttcataattc agcccaccaa tccgccggcg gaaaatatca tggagctcct
     2461 gattatggtg gaagcctgcc gaagagcctc ggcttcgagg attacggccg tgataccgta
     2521 ttatggttat gcccggcagg accgcaagag ccagccgcga gtggccataa ccgccaaact
     2581 gctggccaat cttataaccg ccgccggcgc ggaccgtgtt gtaaccatgg acctgcatgt
     2641 ttcccagatc cagggctttt tcgatatacc ctccgaccac ctttatgcct ccaacctgtt
     2701 ctataatcat ttcaggatgc gcggtatgac agatattgtc gtggtgaccc cggatgtcgg
     2761 ctcgatcaaa attgcccgag cattcgccaa cagcctgcag tgcggcctgg cgattatcga
     2821 caagcgccg
//
LOCUS       DSEB01000166            3107 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003821, whole genome shotgun sequence.
ACCESSION   DSEB01000166 DSEB01000000
VERSION     DSEB01000166.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003821"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            86..>3107
                     /locus_tag="ENO07_03045"
     CDS             86..>3107
                     /locus_tag="ENO07_03045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HDS00972.1"
                     /translation="MAKEDPQKRNTGSKKVVQTRMPAGPQSSAYSATLKLMENKHYAV
                     LIMDPGNLRIVFANSKLCQKLGYSKSELIGRKVTSFIAPEDRKRIVETAAARLEGKPV
                     ETGNRYTALTRNGGRIVFDAYTNPVQYDGRRYLQTITNDITQQVKLEQELRESEEKYR
                     AVVDNIADGIAVCASGKLAYSNKMFAQIFGYKPAEIEGIQINKLIARGYKKLLTGLQK
                     QVWESAKRRKLEIVGLKKDNSEIILNLRASRIKYHGKKALQLTITDITAEKEAEAQLR
                     ENEERFRSLVESVQYGFFILEVKASKLLFLNNYGRSLASGVKTPIDELDIFGFLEPSD
                     RKNIRDKCEQIISGSLDGFSGVQKVIFPGQEPIWIQYESRVVPFKGRICLQGIFKDIT
                     EVKVADDMLRESEERFRQMAENIPELFWIVERNPDKTVYVSPGARDVFGLSQEELYDN
                     SRSFLRAIHPDDRDWVAERFDDIENENDVEFRVRSDSGAVRWVRALTFPIRDQDGKVY
                     RTTGIVRDITSRKRTEQQLQASEARYRAVVEDQTELITRSRPDGTITFLNDACCRFFG
                     MEKSESYEGSFLDYVHPDDRERIQETIQSVSFENPVVSVEHRVFNKNGEVRWQHWTNR
                     MVFDDEGNMQEFLSVGRDITEQKMAEKALRESELKFRAIFENTSDGIAIYESHSKKFA
                     QKLIDCNDSFVKMSGRSKEELLDAKDVREFRLNEDRELDEHEMQKLILAGVAFKGTYS
                     WKRPDRKENFIEYEAAPVILGERILVYTIDRDITERRKNEIRLSESEKKYRKLYESLM
                     DGYARTDLDGRILEFNVAFQKMIEYPPDEIMGLTYHDITPEKWHEFERDLVDNQVMIH
                     GYSKTYEKEYRKKSGHIFPVELRTYLTYDDFGLPDGLWAIVRDISERKAVEEQLRESE
                     EKYRSVVDNVGIGISLISPDMRILSLNNQMKKWFPKINVEEHPVCYKAYNDPPRSCIC
                     FYCPTIRTLRDGGVHEDITETPSGGTIRNFKIVSS"
BASE COUNT          924 a          621 c          819 g          743 t
ORIGIN      
        1 ctataaacca gaatccattt gaatattgaa gactaactgc gtatattcat gtagcaaaaa
       61 gcatgaacgt gaagacctgg taaatatggc taaagaagat ccccaaaaga gaaataccgg
      121 atcaaaaaag gttgtgcaga caagaatgcc ggccggtccg cagtcctctg cgtacagcgc
      181 cacccttaaa ctgatggaga acaagcacta tgccgtgtta attatggatc ccgggaatct
      241 caggattgtc ttcgcgaact caaagctgtg tcagaagctg ggctattcca aatctgaact
      301 tatcgggcgc aaggtgacct catttatcgc tccggaagac agaaagagaa ttgtggagac
      361 tgcggcggca cgcctggaag gcaaacctgt tgagacaggc aatcgctata ccgctttgac
      421 ccgcaacggg ggtagaattg tttttgatgc ctataccaat ccggttcagt atgacggtcg
      481 gagatatctt cagacgataa cgaatgatat tacccagcaa gtcaagctgg agcaggagtt
      541 acgggaatcg gaagaaaaat accgggcggt ggtagataat atagccgacg ggatcgcagt
      601 ctgtgcaagt ggaaagctgg cctacagcaa taaaatgttt gctcagattt ttggctataa
      661 gcctgctgaa atcgagggaa ttcagattaa taagttgatt gcaaggggat ataaaaaatt
      721 actcacagga ctgcaaaaac aggtatggga atcggcaaaa cgcagaaaat tggagatagt
      781 cggtctgaaa aaagacaact ccgaaatcat tcttaatctc agggcttcgc ggattaaata
      841 tcatggaaaa aaggctcttc aattgaccat cacagatata actgcggaaa aagaagctga
      901 agcgcagcta agagaaaacg aggaaagatt caggtctctg gttgagagtg ttcaatatgg
      961 tttcttcata ttagaagtta aggccagcaa gttgctcttt cttaataact acggacgctc
     1021 gcttgccagt ggtgttaaga cccccattga tgaattggat attttcggtt ttcttgagcc
     1081 atccgaccga aaaaatataa gagataaatg tgagcagata atttccggca gtctggatgg
     1141 attttcgggg gttcagaagg ttattttccc gggtcaagag ccaatctgga ttcaatatga
     1201 atccagggtg gtgcctttca agggccgcat ctgccttcag ggaatattta aagatataac
     1261 ggaagtaaaa gtcgccgacg acatgttgag ggagtcggag gaaagattcc gtcagatggc
     1321 agaaaatata cccgagctat tctggatcgt cgagagaaat ccggataaaa ctgtatacgt
     1381 aagccccggt gcaagggacg tattcggcct gtcgcaggaa gagttatatg ataacagcag
     1441 gagcttcctc agggccattc accccgacga tagagattgg gttgccgaac gctttgatga
     1501 tattgagaat gaaaatgatg ttgaattcag ggtcaggagt gattccggtg cggtgagatg
     1561 ggtgcgggcc ctcacatttc ccatccgtga tcaagacggg aaagtttacc gcacaaccgg
     1621 tattgtcagg gatatcacct cgcggaaaag gacggagcag cagttgcagg caagcgaggc
     1681 gaggtatcgt gccgtggtcg aagatcagac tgaactaatt acccgcagtc gccctgacgg
     1741 cactatcaca tttctgaacg atgcctgttg tcggtttttt ggtatggaaa agtctgaatc
     1801 ttacgaagga agctttttgg actacgtgca tccggatgac cgcgagagaa ttcaggaaac
     1861 tattcaatcg gtatcttttg aaaatccggt tgtctcggtc gagcacaggg tcttcaataa
     1921 aaacggcgag gtccgctggc aacattggac caatcggatg gtgtttgatg acgaaggtaa
     1981 tatgcaggag tttctgtcgg ttggacgcga tatcaccgag cagaaaatgg ccgaaaaggc
     2041 cctgcgggaa tcggagctga aattccgggc gatctttgaa aataccagcg acggtattgc
     2101 tatttacgag agccattcca agaagtttgc ccagaaactg atcgattgta atgacagttt
     2161 cgtgaagatg tccgggagga gcaaagaaga gcttctggat gcaaaagatg tccgagaatt
     2221 ccgtctcaac gaggacagag aacttgatga acatgagatg cagaaattaa tactagccgg
     2281 tgttgcattc aaggggacct attcctggaa acggcccgac cgcaaggaga atttcatcga
     2341 atatgaggcc gctccggtaa ttctgggcga gcgcattctg gtttatacga tcgatcggga
     2401 tattacagag cgcagaaaaa atgagatcag gttgagcgag tctgagaaga aataccgtaa
     2461 actctacgaa agtctgatgg acggttatgc gcgcactgac cttgatggaa ggattctgga
     2521 gttcaacgtt gccttccaga agatgattga atatccaccg gatgagatca tgggtttgac
     2581 ttaccacgat attacccctg aaaagtggca tgaatttgag agggatctgg tagataatca
     2641 ggttatgatc catggttact caaagacata tgaaaaggaa tatagaaaaa agtcgggaca
     2701 tattttcccg gttgagctcc gtacctatct cacgtatgac gatttcggcc tgccggatgg
     2761 tctatgggcg atagtgcggg atatttcaga gcgcaaggcg gtggaggaac aactgcgtga
     2821 atctgaggaa aagtatcgca gcgtggtcga taatgtcgga attggtatat ctcttataag
     2881 cccggatatg cggattctgt ctctgaataa tcagatgaaa aagtggttcc ccaaaattaa
     2941 cgtcgaggaa catcctgtct gttataaagc ctataatgat ccgccacgct cctgcatttg
     3001 tttctactgc ccgactatac gaacactaag agacggtgga gttcatgaag atataaccga
     3061 gaccccctcc ggcggcacta tcagaaattt caagattgtc tcttctc
//
LOCUS       DSEB01000167            3001 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004124, whole genome shotgun sequence.
ACCESSION   DSEB01000167 DSEB01000000
VERSION     DSEB01000167.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004124"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="ENO07_03050"
     CDS             complement(<1..1041)
                     /locus_tag="ENO07_03050"
                     /inference="COORDINATES: protein motif:HMM:PF01523.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDS00973.1"
                     /translation="MEFTDMSKYDKIELARWVVKKGKQAGADDISVDVIKDRSISVDF
                     RDGKMEELKESSQNYLSLSVYADHRYTSHTTCDLNKDSLEKFISEAVAMTGYLNEDPY
                     RSLPDPKYYKIRNDIDLGIHDDHYYDVTSEQRVKIARDLEAATSALSDKIVTCNASYS
                     DSRAEFVKINSNGFEGERQSTYFGVGVETTVKDVHGGRPGDYDYIGSRFFKDISSLER
                     LGRTAFKRAMQKIGQKKIESGVYDMIVENRALTTMLAGIRYPLTGSYLYRKNSCFENK
                     LGEKIASGKLTIIDDPYLKAGFSSQLYDNDGMATKKRALFEKGVLKSYLIDWYYSRKL
                     NMEPTTGSESNIT"
     gene            complement(1076..2608)
                     /locus_tag="ENO07_03055"
     CDS             complement(1076..2608)
                     /locus_tag="ENO07_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDS00974.1"
                     /translation="MTRRSFLKTSAQGLAVAAVPVIFKANPLAALSSPLLNPDSAAEY
                     YEHFEVSEEIIRKVMASALNKGGDYCDMFFEHTISNTILLEDDRVDRAYSNVDYGVGI
                     RVVENDQIGYSFTEELNPAAMGDAARTAANIANQSRSIPPAALDFRKAADFYPIETLW
                     EDVGIKDKIPILRKLNERVWSRDKRIINARVYFVDTSTYKMIVRSDGVVGCDYQPMIT
                     IYVLCTAEDKGKREQNNFSMSVRRGIDHLTSENLNPIADEAVRQTVLLFDAVNLEGGE
                     MEVVLAAGESGILLHEAIGHGMEADFNRKEESIFSDKIGKKVAENFVSIVDDGTYPGA
                     RGTLNLDDECNSTRKTYLVKDGILLSYMHDAISARHYKVEPTGNGRRQSFRYPPIPRM
                     RNTYMMPGPHTKEEIISSVKKGLYAESFTNGEVLIGPGDFTFYLSSGYLIENGKITRP
                     VKDVNLIGNGPDVLTRVVMVADDLKLSEGGGTCGKGGQGVPVSMGLPTVKVSSITVGG
                     RS"
BASE COUNT          691 a          799 c          702 g          809 t
ORIGIN      
        1 ggtaatgtta gattctgaac cggtggttgg ctccatattg agctttcggg aatagtacca
       61 gtcgatgaga tatgatttaa gtacaccttt ctcgaaaagc gcacgtttct tcgtcgccat
      121 gccatcgtta tcatacaact gcgaggaaaa ccccgccttg agatacgggt cgtcgatgat
      181 ggtcagttta ccggaggcga tcttttcgcc cagcttattt tcaaaacatg agttcttacg
      241 atataagtag cttcccgtga gaggatatct gattcccgcc agcatcgtgg tcaatgcccg
      301 gttttcgaca atcatgtcat acacgccgga ttcaatcttt ttttggccga tcttctgcat
      361 cgctcttttg aaagctgttc tgccgagacg ctccagggac gatatatctt tgaaaaatcg
      421 cgagccgatg tagtcgtaat cgccgggccg cccgccatgc acatccttta ccgtcgtttc
      481 cactcccaca ccgaaatagg tggattgcct ttcgccttca aatccgttgc tgttgatctt
      541 cacgaattcc gcccggctgt cagagtaaga agcattgcag gttactattt tgtcgctcag
      601 agcggaggtc gcggcctcaa gatccctggc aatcttgact ctctgctcgg aggtgacatc
      661 gtagtaatga tcatcatgaa ttccgagatc gatatcattg cggattttgt aatatttagg
      721 atcgggaagg gagcggtatg gatcctcgtt caggtagccg gtcattgcca cggcctcgga
      781 gatgaatttt tcaagagaat ccttattcaa atcgcaggtc gtatggctgg tataacgatg
      841 gtctgcgtaa actgaaaggg acagataatt ctgcgaggac tctttgagtt cctccatctt
      901 gccgtcgcga aaatctacgc ttatgctcct gtctttgatt acgtcaaccg aaatatcgtc
      961 ggccccggcc tgcttgcctt tctttacaac ccacctggcc agttcaattt tatcatattt
     1021 gctcatatct gtaaactcca tttacaaatt cattataaga ttatctttga atctatcagg
     1081 atcttccccc cacagtgatc gaggagacct ttacggtggg cagtcccatc gagaccggga
     1141 caccctggcc gcccttgccg caggtgccgc cgccctccga cagcttcaga tcatcagcca
     1201 ccatgaccac cctggtcaac acatccgggc cgttgccgat caggtttacg tctttcaccg
     1261 gcctggttat cttgccgttc tcgatcaggt agcctgagct cagatagaag gtaaagtctc
     1321 ccggaccaat cagcacctcg ccatttgtaa atgactcggc gtaaagtccc ttcttcacac
     1381 ttgaaataat ttcttctttt gtatgcggtc cgggcatcat gtaggtgttg cgcatgcgcg
     1441 gaatcggcgg atacctgaag gactgccggc gaccattacc ggtcggctcg accttataat
     1501 ggcgggcgct gatagcgtca tgcatgtagc tgagcaggat tccgtctttc accagatatg
     1561 tcttccgggt ggaattgcat tcatcatcca gattcaatgt cccgcgggca ccgggataag
     1621 taccgtcatc tacaatcgaa acgaagtttt cagccacctt cttgccgatc ttatccgaga
     1681 aaatggactc ttctttgcgg ttgaaatcgg cttccatccc gtgaccgata gcctcatgca
     1741 gcagaatccc ggattcgcct gcggccagca ccacctccat ctcgccccct tccagattga
     1801 ccgcatcaaa gagcagtacg gtctggcgta ccgcctcatc ggcaatcggg ttcaaatttt
     1861 cggaggtaag gtggtcaatg cccctgcgga cactcatgga gaagttattc tgttcgcgct
     1921 tgcccttatc ttctgccgtg cagaggacat agatcgttat catgggctgg taatcgcagc
     1981 ccaccactcc atccgatctc actatcattt tgtaggtgct tgtgtcaacg aaatacaccc
     2041 tggcatttat gatgcgttta tcccggctcc agactctttc attgagttta cggagtatag
     2101 gaattttgtc cttgataccg acatcctccc agagagtctc gatcgggtaa aaatcggccg
     2161 ccttccggaa atccagggcg gcaggcggta tactgcggct ctggtttgcg atattagcgg
     2221 ctgtcctggc ggcgtctccc atagctgccg gattaagctc ctctgtgaat gaataaccga
     2281 tctggtcatt ctccaccacc ctgataccga ctccatagtc gacattgctg taggcacggt
     2341 ctacccgatc atcttccagc agaatagtat tcgagattgt gtgctcgaag aacatatcgc
     2401 aatagtctcc gcctttattc agggcggagg ccatcacctt gcgaataatt tcctccgaga
     2461 cttcgaaatg ctcgtaatat tcggccgcac tatccggatt caaaagcggc gatgagagag
     2521 cggccagagg gttagccttg aagataacgg gcactgcagc cacggcaaga ccttgggcgc
     2581 tggttttcaa aaatgatcgc ctggtcattg atgacatgtc tttcctccgt caaaatgttt
     2641 atttcaaaat tccctaaatc tccattttcc tgattaagcc ttcataaatg cacggcatgc
     2701 accaggtccg ggagcgcttt aatcgcctgt tacggcggca gatctgaaaa ctgacgccgt
     2761 aacggcatac ccctatccaa actttatata ttcagccagt aactcgcttt tatcaggaat
     2821 acattagtcg ctccggaact gaagaagcga tcgagatccc ggtcgagagc aagcttgttg
     2881 tggtcatagt cgacttccgg acgggcgcgg gtccatacca gatagagcgt gctgccgggc
     2941 atgtactccc atcttaatag caaagaagaa tttatcgccg aataattata atctgtatta
     3001 a
//
LOCUS       DSEB01000168            1501 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1019088, whole genome shotgun sequence.
ACCESSION   DSEB01000168 DSEB01000000
VERSION     DSEB01000168.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1019088"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            353..1099
                     /locus_tag="ENO07_03060"
     CDS             353..1099
                     /locus_tag="ENO07_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00975.1"
                     /translation="MTGRQRITIGICLISILTLTGFFNQGQAEHRENYLVRGSWSMQF
                     GIVQDFTLKAFNGGLISVKRHLSPSTALRMGLTFNGDVGDQSSENLDEYQQFGVQNLV
                     IRTTDGNTSGISLSLSTQLMVYSSIEKSINLYFGIGPNFNYSLDKEEFDVEYIYEDLP
                     ERDGYQDRLLRSWSIGFLGSWGVEWFATRKISFIAEYGARAEYNSVYRERSSLEFVDE
                     ISRRDSFTEDRHDFRFITDAVKFGISLYFN"
     gene            complement(1096..1452)
                     /gene="ytxJ"
                     /locus_tag="ENO07_03065"
     CDS             complement(1096..1452)
                     /gene="ytxJ"
                     /locus_tag="ENO07_03065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HDS00976.1"
                     /translation="MLKDLENIADFDKALQDSRETPVYIYKHSSSCNISRGAWRAVQE
                     LSEEKPELQFRRIMVRENRPLSTDIAQKLRVEHQSPQIILINNGVPIWSATHYGITRD
                     TIMGALKILKSGEEES"
BASE COUNT          401 a          342 c          329 g          429 t
ORIGIN      
        1 catatatttc cttctaactt attctatcgg aacaattcgc cgtaagttca ggatataagc
       61 agttcaacgg caaattgcaa gcagattttc gcctgcccgg ccgaacctgt cttaaacaga
      121 atatttccag aaccgttgca tatttgatac actattatcg attttttaaa atgaaatcga
      181 tttatataga ttttcatggg aatatagcct tcccgtaaat gtagaattat taaagccggc
      241 aatcgccgga agaaacattt cctgattaaa agagcatgag gcatgcttat tgcatcagca
      301 atgcatagag ttaacatatt tcaaatatca ctcataggaa aaggagtcga aaatgaccgg
      361 gaggcaaaga atcacaattg ggatctgtct catatccatc ttaaccctga cagggttctt
      421 taatcagggg caggcagaac accgggagaa ttacctcgtg cgcggatcat ggtcgatgca
      481 atttggaatc gtccaggact tcacacttaa ggcttttaat ggcgggctga tctccgtcaa
      541 gagacatctt tcccccagca ccgctctgag aatgggactg acttttaatg gcgatgtggg
      601 agatcaatcc agtgagaacc tggacgaata tcaacaattt ggcgttcaaa atcttgttat
      661 tcgcacaact gacggcaaca ccagcggaat ttcattgagc ctgtcgaccc agctgatggt
      721 ctactcatcc atcgaaaaat ccataaacct ctatttcggc atcggcccga atttcaatta
      781 cagcctcgac aaagaggaat tcgatgtgga atacatctac gaggatcttc ccgaacggga
      841 tggctaccag gatcggcttc tgcgctcatg gtcaatcggg tttttaggct catggggtgt
      901 ggaatggttc gccaccagga agatcagctt catagcggaa tatggcgctc gtgcagagta
      961 taattctgtt tatcgcgaga gaagctcgct tgaattcgtg gatgaaattt ccagaaggga
     1021 ttcatttact gaagatcgtc atgatttccg tttcatcaca gatgcggtga aattcggaat
     1081 atcgctgtat tttaattagg attcttcctc accagatttc aatattttca acgcgcccat
     1141 tatggtatcc cgcgtgatgc cgtaatgggt tgcgctccat ataggtacgc cgttgttgat
     1201 aagaattatc tgtggcgatt ggtgttccac tctcagtttt tgggctatat cagtggaaag
     1261 cggcctgttt tcacgcacca tgatgcgcct gaattgcaac tcaggtttct cctccgaaag
     1321 ttcctgcact gccctccagg caccgcgcga gatattgcag ctgctggagt gcttgtaaat
     1381 atagaccggc gtttccctgc tgtcctgcag agctttatca aaatccgcta tattttccag
     1441 atccttcagc attgttcctc cacctctgtt attaatcgaa catttccaat ccgattctta
     1501 g
//
LOCUS       DSEB01000169            4477 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001691, whole genome shotgun sequence.
ACCESSION   DSEB01000169 DSEB01000000
VERSION     DSEB01000169.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001691"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..3890)
                     /locus_tag="ENO07_03070"
     CDS             complement(<1..3890)
                     /locus_tag="ENO07_03070"
                     /inference="COORDINATES: protein
                     motif:HMM:PF01364.16,HMM:PF03785.12,HMM:PF08126.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00977.1"
                     /translation="MRVSILASEYTDFENYPVVPSKGNLLRNVNPDDVPYTFGEVYST
                     DAFYPGELANIRDPYIIRDFRGTTIDLNAFQYNPVTQRLRVYTSVTVEIANVGPGVIN
                     VLQNADRTKIVEDFKQIYSRRFINYDFQTDKYTMLTESGDMLIICYDAFMADMQPFVD
                     WKMQKGIKTTMVGTSTAGTTSSAIKSYIQSFFDADSTNLAFVLLVGDAAQIPSPYASG
                     GSSDPSYGKVVGTDVNPDILIGRFSAETVAHVQTQVTKSVEYEQNPYGADWFHKGTGI
                     ASDDGAGIGHNGEADNVHMGYIRDDMLAHTYTLVDEIYEPTGTDVMVTNALNNGRSYV
                     NYCGHGSTTSWSTTGFNNTDVNNLTNDNMLPFIISVACVNGDFDGYTCFAEAWARATN
                     GSEPTGAVASFMASINQSWAPPMWGQDEVCDLLMAETRTTFGSLCFNGSAYVIEQSGA
                     VDISDTWLVFGDPSLQCRTDNPVAMTVNYNPACFFNVPTYDVEVVGVEGALCALYADG
                     VLYGSAYTGADGMATINLNQFLPIGSDVTLTVTAYNKSTFTASITPASDLAIVHTPLQ
                     DTKDTLNDYQVNCTIYSSDTITQNDVILHYDVGAGYVDEVMSQVKIITEDYQAYIPVQ
                     AAGTSISYFITASNQGGFYDTTDVFTFEVIDYQMILEPDEAGVTAPVDDTAWYDMRVT
                     NDGVLADDYTLAVTGDEWPTEIRDATGTTVISSSGTLLGDEYLDFKVMVVVPATLEGE
                     FDEIQLTATSTGEPTLFETTTITTTSAGMPWPIPFSDNFVTTTFDMTKWESVAEAEIN
                     SLGIDEPSAPYSVNLNGQSIGGDAIETEKINLKDESNVIVKYYYQQTGGGESPDAGDD
                     LIIEYLDGTDTWVEINRHLGADPDMTEFEEVELGLPAGAMHAEFRLKIRCTATSGNYD
                     DWFVDDIYVGHPSDYDVRIIPSMQSQYGPAGDSAAYTLTIINKGFLDDNFDLTFDGDW
                     NVAFFDEALSGEINATGVVAGGDSINVIVKVEVPAGTPLHTVNSTTIWATSQGDANMQ
                     AFALVETISAGFPAAIPWYETFPDDTLYTQHWFTFEGAEVTSEAPGTPSQPYSFNLDG
                     GVDTVETQLIDLSGQSGVLLSYYYEMGGMQDPPEYGDNLWIEYLNNAGTWMALHSHEG
                     GGEAMQEFEYVSLELPVDAHHSGLQIRMRTFGSNAGEDNWYVDNIRVDYPPSMSVSGE
                     SMTETLLQGDSAMHEMVIENTGQGGLIYQIDLVPHLRDNFVFAKPAEGQAEPASHDYP
                     EVVYEDVAKGEDINFTGYPVTRGMGGPDNFGYYWV"
BASE COUNT         1073 a         1116 c         1183 g         1105 t
ORIGIN      
        1 acccagtagt aaccgaagtt atccggtccg cccataccgc gtgtcaccgg atagcctgtg
       61 aagttgatat cttcaccctt ggccacatct tcatagacga cctcagggta atcgtggctg
      121 gccggttctg cctggccttc ggccggcttg gcgaagacaa aattatcacg cagatgcggt
      181 accagatcaa tctggtatat caggccgccc tggccggtgt tctctatcac catttcgtgc
      241 atggccgaat caccctgaag cagggtctcg gtcatggact ctccggagac agacattgag
      301 ggtgggtaat cgacccggat attgtcgaca taccagttat cctcgccggc gtttgatccg
      361 aaggttcgca ttctgatctg caggccggag tgatgtgcat ccacaggaag ctcgaggctg
      421 acatattcga attcttgcat ggcctctccg ccgccctcgt gtgagtgcag ggccatccag
      481 gtgcccgcat tattaagata ttcgatccac aggttatcgc catattccgg cggatcctgc
      541 atgccgccca tctcatagta atatgagagc agcactccgg actggccgga aaggtcgatc
      601 agctgggttt ccactgtatc aacaccgccg tcaagattga aactgtaagg ctgcgaggga
      661 gtacccgggg cctcggaggt tacctccgcg ccctcaaaag taaaccagtg ctgagtatac
      721 agggtatcat ccgggaaggt ctcgtaccat ggtatcgccg ccgggaatcc ggccgagatg
      781 gtctctacca gggcaaacgc ctgcatattg gcatcaccct gcgaggtagc ccagatagtc
      841 gtgctgttta cagtatgcag cggtgtacct gcgggaactt ccactttcac gatgacattg
      901 atagaatcgc cgcccgcaac gacgccggtg gcgttaattt caccgcttaa tgcctcatcg
      961 aagaaggcca cgttccagtc accgtcgaag gtaagatcga agttgtcgtc caggaagccc
     1021 ttattgataa ttgtaagcgt ataagcggcg ctgtctccgg cgggaccata ctggctctgc
     1081 atggaaggaa ttatgcggac atcataatct gaaggatgac cgacatagat atcatccacg
     1141 aaccagtcat cgtaattgcc ggaggtagcg gtacatctga ttttcaggcg gaactcggca
     1201 tgcatggcgc cggcgggaag accaagttcg acttcctcga attcagtcat atcgggatcg
     1261 gcgccaagat ggcggttgat ctctacccat gtatcggtac cgtcgagata ttcgataatt
     1321 aaatcatcac ctgcatcagg cgactcgccg ccgccggtct gctggtaata gtatttgaca
     1381 ataacattgg attcgtcttt aagattgatc ttctcggtct cgatcgcatc gccgccgata
     1441 gactggccgt tcaagttgac gctatacggc gcgctgggct cgtcgatacc gagtgagttg
     1501 atttcggcct cggccaccga ttcccatttg gtcatgtcaa atgtggtcgt tacaaaatta
     1561 tcgctaaatg gtatcggcca gggcattccg gctgatgtcg tggttatagt cgtggtctca
     1621 aacagtgtcg gttcgccggt tgaggtcgca gtaagttgga tttcatcaaa ttctccctcc
     1681 agcgtggcag ggacaaccac catgaccttg aaatcgagat actcatcacc caggagagtc
     1741 ccggatgatg atatgaccgt tgtaccggtt gcatcgcgga tttccgtggg ccactcgtct
     1801 cctgtgaccg ccagggtata atcatcggcc agaacaccat cattggtgac ccgcatatca
     1861 taccaggccg tatcgtcaac gggagcggtc actcccgctt catccggctc caggatcatc
     1921 tggtaatcga taacctcgaa tgtgaagaca tccgtggtat catagaaccc gccctggttg
     1981 ctggctgtga tgaagtaact tattgaagtt ccggcagcct gaaccggtat gtaggcctga
     2041 taatcttcgg tgatgatctt gacctgcgac ataacctcat cgacataacc tgcgccaacg
     2101 tcgtaatgca ggatgacatc gttttgggtg atggtgtcgc tggagtagat tgtgcagttg
     2161 acctgataat cgttcagggt atccttggta tcctgcaagg gagtgtgcac aatggccaga
     2221 tcagaggccg gagtgatcga ggcggtaaag gtcgatttat tgtaagctgt aacggttaaa
     2281 gtaacatcgc tgccgatcgg caggaactgg ttcaggttga tggtggccat accgtcagcg
     2341 cctgtgtagg ctgagccgta gagcacgcca tcggcataaa gagcgcagag ggcgccttca
     2401 acgcctacaa cttcaacgtc gtaagtggga acgttgaaga agcaggcggg attgtagttg
     2461 acggtcatcg ctaccggatt atcggttctg cactgcagtg atggatcgcc gaagacaagc
     2521 catgtatccg agatatcgac tgcaccggac tgctcgatca cgtaggcaga gccgttaaag
     2581 cagagcgaac caaaggttgt acgtgtctca gccatcagga ggtcacagac ctcatcctgt
     2641 ccccacattg gcggagccca ggactggtta atagaagcca tgaagcttgc cacagcgccg
     2701 gtgggctcgg aaccgttagt tgccctggcc caggcctcgg caaagcaggt gtaaccgtca
     2761 aaatcaccat tgacgcaggc tacgcttatg ataaacggca gcatgttgtc gttggtcaga
     2821 ttgttcacgt cggtgttgtt gaagccggtt gtgctccagg aggtcgtact cccgtgtccg
     2881 caataattta catatgaacg accattattg agagcattag tcaccataac atcggtgccg
     2941 gtgggttcat aaatttcatc caccaatgta tatgtatgtg ccagcatatc atcacgaata
     3001 tagcccatgt ggacattgtc ggcttcgccg ttgtgaccga tacctgcgcc gtcatcggag
     3061 gcaatgcccg ttcccttgtg gaaccagtct gctccgtatg gattctgctc gtattccacg
     3121 gacttggtaa cctgggtctg gacatgtgcc acggtctcgg ccgagaatct tcctatcagg
     3181 atatcggggt taacgtcagt gccaacgacc ttaccgtagc tggggtcgga tgagccgccg
     3241 gaagcatacg ggctgggtat ctgggcggca tcaccgacaa gcagaacgaa ggccagattt
     3301 gtgctgtcgg catcgaagaa actttgaata tagcttttga tcgccgagct tgttgtaccg
     3361 gcagtggagg taccgaccat agtggttttg atacccttct gcatcttcca gtccacaaag
     3421 ggctgcatat cggccataaa ggcgtcatag cagatgatca gcatgtcgcc ggattcagtc
     3481 agcatggtgt acttgtcggt ctggaaatcg tagttgataa agcggcggga gtaaatctgc
     3541 ttgaagtcct cgacaatttt tgtgcggtca gcgttctgca gaacattgat gactcccggg
     3601 ccgacattgg caatctccac tgtgaccgag gtgtacacac gcagtctctg tgtgaccggg
     3661 ttgtactgga aagcgttgag atcaattgtt gtcccgcgga agtccctgat gatataggga
     3721 tcgcggatat ttgcgagttc tcccggatag aatgcgtcgg tcgaataaac ttcgccgaag
     3781 gtatagggaa catcatccgg attaacattt ctgaggagat tacccttgga tggaaccacc
     3841 gggtaattct cgaaatcggt atattccgag gcaaggatgc tgacccgcat cttgcatcat
     3901 cgggaataat aatgctgcgg cagactcgcg ggagttccgg ttccgcggca ttcaatagaa
     3961 acgactcgtc tccgagtctc aactgataat aggactctcc gttgatatta actgtttcct
     4021 gctcaaagga gccgatatcg aatttgacca cggtcctttg atcgttggat tccagcacct
     4081 caacattgag ctgtttatca gctgccccca gatcgatggt ctctgccgcg agcatgctta
     4141 cggacagaaa aaccacgata gcagccaata gcagtttcgt gcgcatgaaa atccttccta
     4201 ttgattttta tggtgaaatt attttaaaaa tatttatcac gggtaccact ggtaaaggta
     4261 taaataaaaa aatgtcggtc ccaaatgtag caattcatat atatgtatac ggatttaata
     4321 taatataaat ctccgttttg gcaatataaa acagtacaat ccattgactt taagggagaa
     4381 tttcaagcaa tttagtagac ttcgataata gcgatcaggc attttaaggg gtcccatcca
     4441 aaaggcgacc gaaattaaca tccatgttgg gtttatc
//
LOCUS       DSEB01000170            4253 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001916, whole genome shotgun sequence.
ACCESSION   DSEB01000170 DSEB01000000
VERSION     DSEB01000170.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001916"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1065
                     /gene="alr"
                     /locus_tag="ENO07_03075"
     CDS             <1..1065
                     /gene="alr"
                     /locus_tag="ENO07_03075"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDS00978.1"
                     /translation="IKGNAYGHNIRTFVPLAEQCGVRHFSVFSAYEALIAHQSLTEDS
                     HIMIMGDLCASALEWAIENEVSFYIFTVEWLEKTLTAARKIGRPALIHLELETGMYRT
                     GLAGRDLNRAVRIIKANAEYLKVEGICTHYAGAESISNYVRVQEQIHKFKETALDLAR
                     KGLKIKLWHTACSAAALRYPDTRMDLVRFGIAQYGFWPNKETQMHYFMHNGTPRSKKQ
                     LDPLKRVMTWKSRIMNIKEVGKGEFIGYGTSYMTARNQKIASVPIGYFHGFARQLSNR
                     GYVLVRGRRAQVVGIVNMNMMMIDVTDFPEVNIGDDVVIIGSQKKQQITVGSFSDLAQ
                     FLNYEILVRLPEEIPRIVVP"
     gene            1249..1698
                     /locus_tag="ENO07_03080"
     CDS             1249..1698
                     /locus_tag="ENO07_03080"
                     /inference="COORDINATES: protein motif:HMM:PF08327.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HDS00979.1"
                     /translation="MKKQILKLSVDINSPKERVWDVLLQDETFREWTSVFHPGSYAES
                     DWKEGGKALFKTPEGDGMVSKITVHRPAEVISFEHQGILKDGKEDFEDEEAQNWIGFT
                     ETYRISETNGQSRLSIEQDIGEEYLDQFKTLWEKALDKVKEISESRN"
     gene            1739..2254
                     /locus_tag="ENO07_03085"
     CDS             1739..2254
                     /locus_tag="ENO07_03085"
                     /inference="COORDINATES: protein motif:HMM:PF08327.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00980.1"
                     /translation="MEKQIKNNRAANTGTHEIVISRELDAPRALVWKAWTDPEHIEEW
                     WGPEGFSTEVEELDLKEGGKWRYVMIGQDGKRYPSEGVFKEIVKPDRITSTDEFGEDF
                     ENSASLDLPHGIIVTTLFEDLRGKTELTVRIMHRTAEDKKKHQDMGVMEGWNSSLDCL
                     QEYLENNLTEK"
     gene            2268..2753
                     /locus_tag="ENO07_03090"
     CDS             2268..2753
                     /locus_tag="ENO07_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDS00981.1"
                     /translation="MQKITPFLWFDDNAEEAVKFYTSIFANSKIEKTIYYNKSSAGAS
                     GRPEGSVMSIEFTLEGQEFIALNGGPHFSFSPAISFLIKCKPQQEVDGLWGKLSQGGK
                     EERCGWLRDKYGVSWQVVPVGLNDMLDDGDAAKSNRVTEALLKMNKIEMSTLREAYER
                     A"
     gene            2890..3099
                     /locus_tag="ENO07_03095"
     CDS             2890..3099
                     /locus_tag="ENO07_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDS00982.1"
                     /translation="MGLKNHIKQHRARLNLTQEELAQMVGVRRQTIISIEKGKYAPSA
                     VLAFRIAKVLGMKVDDLFQLNEEFE"
     gene            3100..3321
                     /locus_tag="ENO07_03100"
     CDS             3100..3321
                     /locus_tag="ENO07_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00983.1"
                     /translation="MRSEMDILSMDERQRICWLLANRATLMIVGLVWLGMIVWKLIQN
                     QIPYFLLIMVPVFALIRFVMYHVYAAKLK"
     gene            3425..3964
                     /locus_tag="ENO07_03105"
     CDS             3425..3964
                     /locus_tag="ENO07_03105"
                     /inference="COORDINATES: protein motif:HMM:PF13563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HDS00984.1"
                     /translation="MYGIVSELPDPYYQIVEDIWDELERECGLSGIKAAPIPHFSWHV
                     AEQYDINKSAQIIEELASGTSPFRVRTGGIAVFTGENPIIQIPVVRTGEINSLHERIW
                     NAIQPAAKNMVEDFESAHWIPHISLAYPDVDRDNLNCAMQLLAFRDFRWHFNINSISL
                     IGNEIDHEAERVFKIEFGK"
     gene            3983..>4253
                     /locus_tag="ENO07_03110"
     CDS             3983..>4253
                     /locus_tag="ENO07_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HDS00985.1"
                     /translation="MDKDDLMIFSGSASIKLTEKICDYLMVKPARGEVFKFSEGNTFV
                     RVLENVRGRGVYLVQSTVFPSNDAFMELLFWIDALNRASADSVTLI"
BASE COUNT         1237 a          867 c         1074 g         1075 t
ORIGIN      
        1 atcaagggga acgcctacgg gcataatatc cgcacctttg tcccgcttgc tgagcaatgc
       61 ggggtgcggc acttctcggt cttcagcgcc tatgaggccc tgatcgcaca tcagagtctg
      121 actgaggaca gtcatataat gattatgggc gatttgtgtg ccagcgccct tgaatgggcg
      181 attgaaaatg aggtgtcatt ctatattttc acggtggaat ggctggaaaa gacattaaca
      241 gcagccagga agatcggccg gccggccctc attcatctcg agctcgaaac cggtatgtac
      301 cgtaccgggt tggcaggaag agatctcaat cgggccgtca gaattatcaa agccaatgcc
      361 gaatatctga aggtcgaggg tatctgcacc cattatgccg gcgccgaaag tatcagtaat
      421 tatgtgagag ttcaggaaca gatacacaaa ttcaaggaaa ccgcgcttga tctggcaaga
      481 aagggcctga aaataaagct ctggcatacc gcctgttccg cggccgccct gcgatatccg
      541 gacacacgta tggacctcgt gcggtttgga atcgcacagt atggtttctg gcccaataaa
      601 gagacacaga tgcattactt catgcataat ggcactcccc gcagcaagaa gcagctcgat
      661 ccattaaagc gcgtgatgac ctggaaaagc cgaatcatga atatcaaaga ggtcggtaag
      721 ggagaattta tcggatacgg gacatcatat atgaccgcca gaaatcagaa aatcgcctct
      781 gttccgatcg gatattttca cggtttcgca cgccagttga gcaaccgcgg ctatgttctg
      841 gtgcgcgggc ggcgtgccca ggtggtgggt attgtcaata tgaatatgat gatgatcgac
      901 gttaccgatt ttccggaagt caatattggc gacgatgtcg ttattatcgg atcccagaaa
      961 aaacagcaga ttacagtcgg atcattcagc gatctggctc aattcctgaa ttacgaaatt
     1021 ctggtccgcc tgcccgagga aatcccgcgc atagtggtac cttaatgctc cgttcattac
     1081 ccctcctgtt gataaagatt gcattacaaa gtaatcttta ttaagcctca ggatttatat
     1141 ttattttaaa tcgcaatttt ttccaaaact gcacagcgct catttctgtt atagagacag
     1201 taccgaaagt gattacctat tcttgaataa ttaataaagg agttaaatat gaaaaagcag
     1261 atccttaaat tgtctgtcga tataaattct cccaaggaaa gagtctggga tgttcttctg
     1321 caggacgaga ccttcagaga atggacctcc gtctttcatc cgggttcata tgctgaatcg
     1381 gattggaagg agggcggaaa agcgctgttc aaaactcctg agggtgatgg tatggtcagc
     1441 aagataacag ttcacaggcc ggccgaggtc atttccttcg aacatcaggg aatcctcaaa
     1501 gatggtaagg aggactttga agacgaagaa gctcagaact ggataggttt cacagaaaca
     1561 tatcgaatca gcgagactaa cggccagtcg cggctttcta ttgagcagga tatcggcgag
     1621 gaatacctgg accagtttaa gaccttgtgg gagaaggccc tcgataaagt aaaagaaata
     1681 tcagaatcaa gaaactgacc gtgatcgtca gaagattata tagaaagcga ggataattat
     1741 ggaaaaacaa attaagaata atagagcggc aaacactggc acgcatgaga ttgtcatttc
     1801 gcgtgaactc gatgccccgc gtgctctggt ctggaaagca tggactgatc ccgagcatat
     1861 tgaggagtgg tggggacctg aaggcttttc gacagaggtg gaagagcttg atctgaaaga
     1921 gggcggcaaa tggcgttatg tgatgatcgg tcaggatggc aagaggtatc catctgaagg
     1981 agtcttcaaa gaaattgtga aacccgatag gataacctca accgatgagt tcggggaaga
     2041 ttttgaaaac tcggcctctt tagatctgcc gcatggtatt atagtaacca cgctttttga
     2101 agacctgaga ggtaagacag agttgactgt gcgaatcatg catcgcacgg ctgaagataa
     2161 aaagaaacac caggacatgg gcgtcatgga aggctggaat tcgagcctcg actgccttca
     2221 agagtatctg gaaaataatt tgacagaaaa atgagagccg ggaatatatg cagaaaataa
     2281 ctccattctt gtggtttgat gataatgccg aagaggcggt gaagttttac acttctattt
     2341 ttgcgaactc caaaatagaa aaaactatct actacaacaa atcgtctgcc ggggcctcgg
     2401 ggagacctga gggatcggta atgtcgatcg aattcacgct cgagggacag gaattcattg
     2461 ctttgaacgg cgggccgcac ttctcattct ctccggctat atcatttctc ataaaatgca
     2521 aaccccagca ggaagttgac ggattgtggg gaaaactctc ccagggtgga aaagaagaac
     2581 ggtgcggctg gctaagagac aaatatgggg tctcctggca ggttgtaccg gttggcttga
     2641 atgatatgct ggatgatggt gatgctgcca aatcgaacag ggtaacagag gcattgctta
     2701 aaatgaataa gattgaaatg agcactttga gggaagctta cgagcgggcc tgatcgggct
     2761 gcatctgtca aaagaccgac aacaactaaa gaatagacac aaaatcttta tattttaaaa
     2821 aaaagtaaaa attgcttgac gtaatgtaag ctatacatta cattatgtaa tgtattaatt
     2881 acaattatga ttggattaaa gaatcatatc aagcagcatc gagcgcgtct gaacctgacg
     2941 caggaggaac tggctcaaat ggttggagtc aggagacaga caataatctc gattgagaag
     3001 ggaaaatatg ccccctccgc agtgctggca ttcagaatcg ccaaagttct tggtatgaaa
     3061 gtggatgatt tatttcaact taacgaggag tttgaataga tgagatcgga gatggatatt
     3121 cttagtatgg atgaacggca gcgtatctgc tggctgttgg ccaaccgggc gaccttgatg
     3181 attgtgggac tggtctggct gggcatgatt gtctggaaat tgattcaaaa ccagattcca
     3241 tatttccttt taatcatggt cccggttttt gccttgatcc gttttgtgat gtatcatgtt
     3301 tatgccgcaa aactaaaata gaaaccctaa tccgcttaat attgttgact ttccgcaaac
     3361 tttaccgcac tttttttctg attgatcaaa agatctaatt tcatgaggat tataattaag
     3421 aattatgtat ggcatagtat cagaactgcc ggatccatat taccagattg ttgaggatat
     3481 ctgggatgaa ctggagaggg agtgcggtct cagcggcatc aaagcagccc ccataccgca
     3541 tttttcatgg catgttgccg agcagtatga tatcaataaa tctgctcaaa tcatagaaga
     3601 actggcatcc ggcacgtcac cgtttcgcgt gcgcaccggc ggaattgccg tgtttaccgg
     3661 cgagaatcca attatccaga ttcctgtggt caggacaggt gagataaaca gcctgcacga
     3721 aaggatttgg aatgcgatcc agccggccgc aaagaacatg gtggaggatt tcgagtctgc
     3781 acactggata ccgcacatat ctctcgccta cccggatgtt gacagggata acctgaactg
     3841 cgccatgcag ctgctggcgt ttcgtgattt tagatggcat ttcaacataa attccatttc
     3901 attgattggt aatgagatag accatgaagc tgaaagggtc ttcaaaatag aatttggaaa
     3961 ataacaagcg gggagaatat ttatggataa agatgatttg atgatatttt ccggctcggc
     4021 cagtatcaaa ttgaccgaaa aaatatgtga ttatctgatg gtcaagcctg cccggggcga
     4081 ggtattcaag ttctctgaag gcaatacttt tgtcagggta cttgaaaatg tgaggggccg
     4141 tggtgtgtac cttgtccagt ccacagtttt tccctccaac gatgccttca tggaactatt
     4201 attctggatc gatgccttga accgtgccag cgccgattct gtgaccctaa taa
//
LOCUS       DSEB01000171            4262 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001910, whole genome shotgun sequence.
ACCESSION   DSEB01000171 DSEB01000000
VERSION     DSEB01000171.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001910"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..885)
                     /locus_tag="ENO07_03115"
     CDS             complement(<1..885)
                     /locus_tag="ENO07_03115"
                     /inference="COORDINATES: protein motif:HMM:PF00656.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HDS00986.1"
                     /translation="MRKPAILLIATGILLCLALSCSESPVDSNPDSQKAAIVLTPRPE
                     FVDIRPAEVIQSYLIAPSQQFVDALEKKPPKPPPDTGSQDPNPNPAHKYAYIVGISDY
                     DGTVNDLNFCDDDAQAMKSYLQGQGFTVQMDLDQSATADNIQAGLQWLEAQAAAGDEI
                     AFCYSGHGTKYPGYGSCIISTDMYYMTHGFVMQYINSAACTKKLVTLDACVIGDFHLD
                     CQAGTFMATASTNSDSYDVYAFEHGAWTYFFLEALNDLNYVYAEEVAPYAEAEMKAWA
                     KPYHLRVSPAHTDAYEGMF"
     gene            1032..1511
                     /locus_tag="ENO07_03120"
     CDS             1032..1511
                     /locus_tag="ENO07_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HDS00987.1"
                     /translation="MGSNNEKILRDYLARMLNWQDAHVNLEKAVENFPPDLMGKKPEG
                     AAHSAWQLLEHMRLAQWDILDFIKNPDYEELNWPDDYWPKDPAPPDDSAWDKSIESFN
                     ADLKELQDMARDSSVDLYSKIPHGSGQTILRELLLVADHNTYHLGQIVQLRKMLGAW"
     gene            complement(1629..1931)
                     /locus_tag="ENO07_03125"
     CDS             complement(1629..1931)
                     /locus_tag="ENO07_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00988.1"
                     /translation="MKAAIIFTGTGPILILTSYESFTDPKFVETLSAKGIKRFIACEI
                     PLERVKEKYASTFETVMNDVKQVDDLRVLDYNGHNVFHNFSFSEMGKEVKYEPQPT"
     gene            complement(2197..2838)
                     /locus_tag="ENO07_03130"
     CDS             complement(2197..2838)
                     /locus_tag="ENO07_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00989.1"
                     /translation="MRALIIPAVICLCLFWPSADCSADMDIGLSIGDDGLKSFYLAIG
                     EHYRAPEKELIVVRRSKIPEEEMPVVFFLARKAEVSPGLIVQLRLEGKSWWQITAHFG
                     LNAGVYYVPVEGKPVPPYGKAYGHYKNKKKSQWGEIVLSDADIVNFVNLRFISEHYGY
                     APEEVIKMRANGHNFVQINTQVKNKKNKSKTEKYAEDQEPARNNKSQGKNKKK"
     gene            complement(3053..4252)
                     /locus_tag="ENO07_03135"
     CDS             complement(3053..4252)
                     /locus_tag="ENO07_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDS00990.1"
                     /translation="MEKLIYLDNAATSWPKPDKVYDFMIEFYRGCGVNPGRSGFDKAI
                     EAGNIVEDLRKTLTKFFGGDEDAPERLCYSYNATDALNLIIQGLLSEGDHVVTTNLEH
                     NSVIRPINHLVRDHGVEATYVPFNKDGFVEPDAIKAAIKPNTKLVIVNHGSNVIGTIQ
                     PVAEIGAVCKEMDVLFAIDVSQTAGVVPINMKEMNVDVLAFTGHKGMMGSTGIGGLCV
                     RKHVEIKQTRSGGTGVRSAYPYHLEEYPYRMEFGTPNMVGIASLWAGQDWIREQGGVD
                     VIYKHEMKLAQKLADGFKKIDGVEVYCCGSMENHLATMTINVKDMEAGNVGIMLDVDF
                     SIATRTGLHCAPLVHKQLGIDKIHGGVRFAIGPFNTGEDIDAAINAVDEIAQRARQMK
                     KSVSSAS"
BASE COUNT         1064 a         1091 c          979 g         1128 t
ORIGIN      
        1 aaacatacct tcatacgcat ccgtgtgcgc cgggctgacc cgcaggtgat atggtttcgc
       61 ccaggccttc atttcagcct cggcataggg agcaacctct tcggcataaa cataattcaa
      121 atcattcaaa gcctccagga agaaatatgt ccaggcacca tgctcaaaag cgtagacatc
      181 ataggaatct gaattggtcg aggctgtggc catgaaagtt ccggcctggc aatctagatg
      241 aaagtctcca ataacacagg cgtcaagcgt caccagcttc ttcgtgcatg cggcggaatt
      301 aatatactgc attacaaagc catgcgtcat ataatacatg tcagttgaaa ttatgcatga
      361 gccatagccg gggtacttgg taccatgacc cgaatagcag aatgcgatct catccccggc
      421 ggcggcctgg gcctcaagcc attgcagacc ggcctggata ttgtcggccg tggcgctttg
      481 atcgagatcc atttgaacgg tgaaaccctg gccctgcagg tagctcttca tcgcctgagc
      541 atcatcatcg cagaaattca aatcattaac cgtcccgtca taatcagata ttcccacaat
      601 ataggcatac ttatgggctg gattgggatt cgggtcctgg cttccggtat ccgggggcgg
      661 tttgggcggc ttcttttcca atgcgtcgac aaactgttga gaaggcgcaa tcagataaga
      721 ctgaatcacc tccgccggtc ttatgtcgac gaattccgga cgaggggtca ggacaatcgc
      781 tgctttctgg ctgtccggat tgctgtcgac aggagactca ctgcaggaca gtgccagaca
      841 aagcaaaatc cctgtggcaa tcaaaaggat tgctggtttc ctcatttctc ctcctgttta
      901 tttaagattt gggctttaga tagcatatat atatactgta tcatctattt tgtaccaaaa
      961 taaaaattaa tggtcctgtc tcttttatga atccaggaac aaaaacctga aattcagatt
     1021 ctaaaaatta aatgggttca aataatgaaa aaatattgcg tgactatcta gcccggatgc
     1081 tgaactggca ggatgctcat gttaacctgg aaaaggctgt ggaaaatttt ccgcctgacc
     1141 tgatgggaaa aaagccggaa ggtgcggcgc attcggcctg gcagttactg gaacatatgc
     1201 gcctggccca atgggatatc ctggatttca tcaaaaatcc agattacgag gagctgaatt
     1261 ggccggatga ttactggcct aaagaccctg ctcctcctga tgattccgcc tgggacaaaa
     1321 gcatcgaatc attcaacgct gatctcaagg aactccagga catggccaga gacagctcgg
     1381 tcgatctcta cagcaagatt ccgcatggca gcggtcagac catactgcgg gagttattgc
     1441 tggtggccga tcataatact tatcacctgg gtcaaatagt gcagctgcgc aaaatgctgg
     1501 gcgcctggta agagatttct ctgctttagt ccagaagacc aatctatttt aaatgcccgg
     1561 cggacctcct tatccaccgg gcattttttc ccgtgcacga gccaccaaag aataccccag
     1621 cgacagccct atgtcggctg aggttcatat ttgacttcct tgcccatctc tgaaaatgag
     1681 aagttgtgga agacattatg accgttgtag tccagcaccc gcagatcatc aacctgcttg
     1741 acatcattca tgacagtttc gaaagtggag gcgtatttct ctttgacgcg ttcgagtgga
     1801 atttcgcagg cgataaagcg tttaattcct ttggcgctca aagtctccac gaatttagga
     1861 tcggtaaatg actcatagga agtcaaaatc agaattgggc ctgtgccggt aaaaataatt
     1921 gcggctttca tctgacggcc tccattatta aggtttttat caaagccgga gtccaatcaa
     1981 gatgtaataa attctctgat gctgtatcag gtgggtttgt ctctcacctg taatttatct
     2041 ggaatccgcc atcctggcaa gcatttttca ctgatcttgt gataatcaca taaaaggcat
     2101 cctttgtcag tttaaatacc ggggattgaa tccggccttc ccttcgggca aaaaaaatcc
     2161 cgcactcggc gggatttttt caagcgtaaa atcgaattat ttcttcttgt tcttaccctg
     2221 actcttatta ttcctggcag gttcctgatc ttcggcatac ttttcggtct tggacttgtt
     2281 ctttttgttc ttcacctgtg tattgatttg aacaaaattg tgtccattgg cgcgcatctt
     2341 gataacctcc tccggtgcat agccataatg ctcggagata aagcgcaggt tgacaaaatt
     2401 aacgatatcg gcatcggaca acactatttc cccccattgg ctcttcttct tgttcttgta
     2461 atgcccgtaa gccttgccgt agggcggcac aggcttgccc tccacaggca catagtaaac
     2521 gcccgcattg agcccgaaat gtgccgttat ctgccaccag gatttccctt caagccgcag
     2581 ctggacaatc aaccccgggg aaacttccgc ttttctcgcc aaaaagaaca ccaccggcat
     2641 ctcctcttcg ggaatcttgc tgcgtctgac aacgattagt tctttctcag gcgctctata
     2701 atgttcccct attgccagat agaagctctt caaaccgtcg tccccgatcg aaagaccgat
     2761 atccatatct gccgagcaat cggctgaggg ccagaacaaa catagacaaa tcactgccgg
     2821 aattattaaa gctcgcatat tttcctccac aattgcggtg atactgcaca ataaaaccgg
     2881 gttctggaaa cccgtgacac ctacctttat attgtcggag ggattatatc cgattcttaa
     2941 gaaaattaat ttatattccc gagcccgaag ccaaacctgt tgtaattcaa gcggataaat
     3001 aaaaaacccg gcgtgaatga aggccgggct tgattttttg aaagtcgaga tttcagcttg
     3061 cggatgatac cgattttttc atctgacggg cgcgctgggc gatctcatca acagcattta
     3121 tagccgcatc gatatcctct ccggtattga acggcccgat tgcaaaccgt accccgccgt
     3181 gaatcttgtc gattccgagc tgcttatgaa ccaacggagc gcagtgtaat ccggtgcggg
     3241 tggcgatgct gaaatccaca tccagcatga tacccacatt tcctgcctcc atatccttga
     3301 cattgattgt catggtcgcc agatgatttt ccatactgcc acagcagtaa acctcgacgc
     3361 cgtcgatctt cttgaaaccg tcggccagct tttgagcaag tttcatctca tgcttataga
     3421 taacatcaac gccgccctgc tccctgatcc agtcctgtcc ggcccagagc gaggcgatcc
     3481 caaccatatt gggggtgccg aattccatcc ggtagggata ctcctccaga tggtagggat
     3541 aagccgagcg taccccggtg ccgccgcttc tggtctgctt gatttcgaca tgcttgcgca
     3601 cacatagacc gccaattccg gtcgagccca tcattccttt atggccggta aaggccagca
     3661 catcgacatt catctctttc atgttaatag gcaccactcc ggcggtctgg gagacatcga
     3721 tcgcaaagag cacatccatc tctttgcaga cggcgcctat ttcggccacc ggctggattg
     3781 taccgatgac attggagccg tgatttacta taacgagctt tgtatttggc ttaatggctg
     3841 ctttgatggc atccggttca acaaaaccgt ctttgttaaa gggcacataa gtagcttcca
     3901 caccatgatc gcgcaccaga tgattgatcg gtcggatgac cgagttgtgt tccaggttgg
     3961 tggtaactac atgatccccc tcggacaata gcccctggat gatcaggttc agggcatcgg
     4021 tggcgttata ggaatagcac agccgttcgg gagcgtcctc atctccgcca aagaatttgg
     4081 tgagcgtctt gcgaagatcc tcaactatat tgccggcttc tatggcctta tcgaaaccgc
     4141 ttcgtcccgg attgaccccg catccgcggt agaattctat catgaagtcg tagactttat
     4201 ccggtttggg ccatgatgtg gccgcgttgt cgagatatat cagtttttcc acatgtgact
     4261 cc
//
LOCUS       DSEB01000172            9518 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000330, whole genome shotgun sequence.
ACCESSION   DSEB01000172 DSEB01000000
VERSION     DSEB01000172.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9518)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9518)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9518
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000330"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            64..1911
                     /locus_tag="ENO07_03140"
     CDS             64..1911
                     /locus_tag="ENO07_03140"
                     /inference="COORDINATES: protein motif:HMM:PF01640.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00991.1"
                     /translation="MVVPVLKELPPVKAYSDNSNLDVNAEGGFAVLLKDVLMERVRLY
                     VDYYGSMEASQASKSEKLFDEINGLKWSQYTLPESEFVNRLGKDVDPGEEGEALLSTS
                     WHQGGPYNNDCPIGDGGRTVVGCVATAAAQIMAYYQWPPHGQGSYSYYWNGDQSCEGT
                     TDGRTLSADFTDPYDWGNIVDNCNDGCDDEQAAALAHLNREVGIAFNMDYGVCGSGTW
                     TYLALTVFPEYFYYDPSIDREWRSDHTQDAWFNLVKEQILLGHPMEYRIYSHAIVCDG
                     WRELDGVKQYHFNYGWNDGHNAWYNLDNLHCPWAGCGLDEEYMIRNIFPKPDADGDGI
                     LNDSDNCVLTFNADQKDDDGDEVGNDCDNCPDTPNFDQNDNDGDFLGDACDPDDDNDG
                     IPDEEDNCPFLATANTDDYDGDGVGDACDNCYDIQNPYQYDEDGDGVGDACDGLLHMQ
                     AYEVPDGLMGEEYSYQFWAVGGVPPYTWEKSWGQLPYGLTFEAGESGLLAGTPTWASD
                     YSFRIVVTDSDSPAGSDTMLINIMITEPPPPAYICGDASGDEVVNISDAVYIINYIFT
                     GGAAPEPVAAADGNCDGSCNVSDAVMIINFIFSDGGAPCDTNNDGQPDC"
     gene            2061..2960
                     /locus_tag="ENO07_03145"
     CDS             2061..2960
                     /locus_tag="ENO07_03145"
                     /inference="COORDINATES: protein motif:HMM:PF03446.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDS00992.1"
                     /translation="MSIKDKKIGFIGLGTMGAPMARNIKKAGFSLGVYNRTESRAEAF
                     RDEGAAVYYAPWELAEDCDIIIIMVTGPEALLEITGAIQEVLKTGKTVINMSTVSIDA
                     TMQAAEIVQSRGAEFVDAPVSGSKKPAEEGALVILAGADSRLLDELQPLFESMGRDIV
                     CCGEVGYGTRMKLVINMLLGNMMASFAEALVFGRRTGLAFETILQTITAGALGSPLFK
                     IKGQAISEGNYTKNFPVDLIFKDLNLVLHEAGKVGTPLPLASATREAYSGTVGLGLGN
                     EDMAALVKYYEKIADIKIVDGGK"
     gene            2962..4377
                     /gene="aspA"
                     /locus_tag="ENO07_03150"
     CDS             2962..4377
                     /gene="aspA"
                     /locus_tag="ENO07_03150"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDS00993.1"
                     /translation="MKYRLESDSLGERRVPREVYYGIQTARAVQNFPMTGITIAQFPN
                     LIKALASVKKAAAMANMELGILEHKIGDAIVRACDEIRNGTLSEHFVVDVIQGGAGTS
                     TNMNANEVIANRALEIMGHKRGEYQHCHPNNHVNLSQSTNDVYPTALKIASVWDIEKL
                     LKAMDKLKGAFEEKGGEFADILKIGRTQLQDAVPMTLGQEFEAYGITLGEGVKALKDA
                     TREICGINMGATAIGTGINTVEGYAEKVRFLLADITGLPLYTEPNLVEATSDTGAFVQ
                     LSGVLKRVAIKLSKICNDLRLLSSGPRAGIGEINLPSRQPGSSIMPGKVNPVIPEVVN
                     QVCFQVIGNDVTVTLAAEGGQLELNVFEPILGFNIFQSIEMLERACTVLRERCILGIT
                     ANRGRCQQLVESSVSLVTALSPFIGHENAARIARKALETGRPVRELALESGLIDEERL
                     KDVLDPRKMTHPRSITGSGKT"
     gene            4954..5856
                     /locus_tag="ENO07_03155"
     CDS             4954..5856
                     /locus_tag="ENO07_03155"
                     /inference="COORDINATES: protein motif:HMM:PF04055.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDS00994.1"
                     /translation="MLKGIHFLLTYKCTYECDHCFLYCGPHEKGTFTLKNVEAVLEQA
                     GDLGTVKTIYLEGGEPFLFYPMMLESARRADEMGFKIGFVTNAYWATSEEDAELWLKP
                     LLELNISDLSISDDELHGSPDAAGNALRAAKKLGFASGSICIEKPVTLANQSESGQKG
                     EPVVGGDVLFKGRAADKLTENLPRISYKNFKECPHEELVSPGRVHVDPYGNVMLCQGL
                     CIGNLFQKPLSDIMKDYDHSNHPICGPLLSGGPARLAEKYGVEIESGYVDACHMCFLV
                     RRALLKRFPQFLAPPQVYGTTENE"
     gene            6309..>9518
                     /locus_tag="ENO07_03160"
     CDS             6309..>9518
                     /locus_tag="ENO07_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00995.1"
                     /translation="MMRKFGVIVLLAVFLLTSITAVAKTSNSSAPPGYKRIYIEKPML
                     FGVDDNYYDPGPSLKFYSPPPDYFPAGNWRQVYELETTQHDRQHYSSSGRQIVYAGYW
                     NAGCDYIYMNWSGRMVIGSSIILQCKTAIFDWAGGVWDYTAGGSFQAGPSGSFQNLPY
                     TFAPNIGITPDNPWAVSSMSVRNEAEAWKSYIAYGTAGCPTSSYLTHQALPGPPNLEG
                     ITNGYCDENLGDETVSPYLWPIIDLDTIGDVVITHLVHQEWGFCDASQADANNIDAAS
                     LIYQRKIATSAGSPETGTWDGPHFIDSVYNIAYIVRADHRPGNHNVYVAYMKPMYYQY
                     DGAGGSPCAGTGAYQLHQDIVYVESTDDGDNWSAPIYVTDFKSGYEDDRTQPAAYEIT
                     GFVDPTGVFHLVWTAINNDEACSFPVTAKLYHWDNSNNCISLVYDGSEPALFPGWSGG
                     INAWNLMLAKPNISWCDDKLYVSFVRYGAHTTTNDNGELVVDDICGTSSLANTDILVA
                     ASDATGSMGQVWSDAVNVTNTITDSCLTGECESEHWATMAMYSTDSLMIEYILDLEAG
                     SRAGPNDPNEFTQTWTDNPVIFMTWPCFTMADLPQNNCISTSPSTATFDEVALAPDGN
                     TAPPCNTPASVSKDVIVANCGNVNYTYSAASNDGWLTIDGGASGSINAGTGPNYSDNP
                     AWGGAPACAAPATISWTASSATLGEGNYTGTISVDIVEAGVDDFDITVNLVVTCEYYV
                     PEYARITGGCWSVDLWNTPQAAAQTDPAGNMQFYACGGDSITPLYHEALAVGWQEGGV
                     IKMYSDAVTQDSGQLHMRALSDITTSEVGDPGAGSGYYKTQGYWSTPDSSVQGMIEYF
                     VPGHQDTSVLIEKVTLWNTAGVSLGNFILGEESDWDCDPDSSVDEGHIDLDYEMVYMT
                     GDHNGTNIAAGLRPFSGTNSAYGARAINGYDYAYYTLGWYPDTLYNKLQSVSGAFSLF
                     TDSVNGTEMRILNVFYGGTLGANDTLEICKVKAVSLTGESGLRDLMDKGFTFIENYEI
                     VPPPPDCQGRCGDANNDGSVNVSDAVWIINFAFVGGDPPQ"
BASE COUNT         2361 a         2383 c         2509 g         2265 t
ORIGIN      
        1 caacaattga ttgtcaacga caccttgttg gcaaatgtgt attcgattga gcccgggggt
       61 tatgtggttg ttcctgtatt aaaggagttg ccgccggtca aggcctattc tgataattcc
      121 aaccttgatg tcaatgctga aggtggtttc gccgtcttgt tgaaagatgt tttaatggaa
      181 cgtgttcgcc tttatgtcga ttattatgga agcatggaag cctcccaggc ttcaaaatcg
      241 gaaaagctgt ttgatgaaat taacggctta aaatggtctc aatatacgtt acctgaatcg
      301 gaattcgtca acaggctggg caaggatgtt gacccgggcg aggagggcga agcccttctc
      361 tccaccagct ggcatcaggg cggtccatat aataacgact gcccgatagg tgacgggggc
      421 aggaccgtcg tcggttgtgt cgccactgcc gccgcacaga tcatggcata ctaccaatgg
      481 cccccgcatg gtcaggggag ttattcctac tactggaacg gcgatcagtc gtgcgaaggt
      541 accactgacg gccggactct tagcgccgat tttacagatc catatgactg gggcaacatt
      601 gttgataact gcaatgacgg ctgtgatgat gaacaggccg ccgccctggc acacctcaac
      661 cgtgaggttg gaattgcttt taatatggat tatggagtct gcgggtccgg cacctggaca
      721 tatttagccc tcactgtctt ccccgaatat ttctattatg atccctcgat cgatcgggaa
      781 tggcgcagcg atcacactca ggatgcatgg ttcaacctgg taaaggaaca gattttgctg
      841 ggacatccga tggaatatcg catttattct catgcgatag tctgtgacgg ctggcgtgag
      901 ctggatggag tgaagcagta tcactttaat tatggctgga atgacggcca taacgcctgg
      961 tataaccttg acaacctcca ttgcccctgg gccggctgcg ggctggatga ggaatacatg
     1021 atcagaaata tattccccaa gccggatgct gatggcgatg gcatactaaa tgacagcgat
     1081 aattgcgtgc tgacttttaa cgctgaccag aaagatgatg acggggatga ggtcgggaat
     1141 gactgcgata actgccccga tacccccaat ttcgaccaga atgacaatga cggtgatttt
     1201 ctcggggatg cctgcgatcc ggacgatgat aatgacggca tcccggacga agaggacaat
     1261 tgcccgtttc tggccaccgc caatacggac gattatgatg gtgacggcgt gggtgatgcc
     1321 tgcgacaact gctatgacat ccagaatccc taccagtatg acgaagatgg tgatggtgtc
     1381 ggggatgcct gcgacggttt gctccacatg caggcttatg aggtgccgga tggcctgatg
     1441 ggcgaagaat acagctatca gttctgggct gtcggcggag ttccacccta tacctgggag
     1501 aaatcctggg ggcagctgcc gtacgggctt acatttgaag cgggcgagtc cggcctgctt
     1561 gcgggaactc ccacctgggc ctccgattac agcttcagga ttgtagtcac agactcagat
     1621 tccccggccg gtagtgatac aatgctcata aatatcatga taaccgaacc gcctccgcct
     1681 gcctatatct gcggagatgc gagcggggac gaagtagtaa atatttcgga tgcggtatat
     1741 ataataaatt acatatttac cggtggcgca gcgcctgagc ctgttgcggc cgccgacggc
     1801 aactgcgacg gatcatgcaa tgtcagcgat gcggtcatga ttataaactt catatttagc
     1861 gacggcggcg ccccctgcga caccaataat gatggacagc ccgactgttg agtgcataaa
     1921 atagaaatta atgcccgtcc ggatcaattc gagcgggctt tttctatatt ctgacttttt
     1981 agaaaccgat tgcggcattt cggatcgtct gttataatcc atcaaatagc taatctgata
     2041 ttcaaaagga ggataattct gtgtcaataa aggacaaaaa aatcggattt atcggcctgg
     2101 ggaccatggg tgcgccaatg gccagaaata tcaagaaggc cggtttcagt ctgggagttt
     2161 acaaccgcac cgaatcccgt gccgaggctt tcagggatga gggagccgcc gtttattatg
     2221 ccccctggga gctggcagag gattgtgata tcatcataat tatggttacc ggccccgagg
     2281 ccctgctcga gattacaggc gcgatccaag aggtactcaa aaccggtaag acagtcatca
     2341 atatgagcac ggtttcaatt gacgccacca tgcaggcggc agagattgtg cagtccaggg
     2401 gagcggaatt tgtagatgcc cccgtctccg gcagcaaaaa gccggctgaa gagggtgccc
     2461 ttgtgattct tgccggggca gattccaggc tgctcgacga gcttcagcct ctcttcgaat
     2521 ctatgggcag ggacattgtc tgctgcggag aagtcggata cggcacccgc atgaaattgg
     2581 tgattaatat gctccttgga aatatgatgg catcattcgc cgaagcgctt gttttcggca
     2641 ggaggacagg actggcattc gaaactattc tccagacgat tactgcgggc gcactcggct
     2701 cccccctgtt caaaataaag ggacaggcaa tatcggaggg taattacacc aaaaacttcc
     2761 cggtcgacct gatctttaaa gacctgaacc ttgttctgca tgaggccggt aaagtcggca
     2821 cacccctgcc gttggcatca gctactcgcg aggcctacag tgggacagtc ggtctcgggc
     2881 ttggtaatga ggatatggcg gctctggtca aatactatga gaaaattgcc gacattaaaa
     2941 tagtcgatgg aggaaaatag gatgaaatat agactcgaga gcgacagcct gggagaacgt
     3001 cgggtgccga gggaggttta ttatggaatt cagaccgccc gcgcagtcca gaatttcccc
     3061 atgacaggaa ttaccatcgc gcaatttcca aacctgataa aagccctcgc atctgtcaaa
     3121 aaagcggcag ccatggccaa tatggagctc ggtatcctcg agcataaaat cggcgatgcc
     3181 atagtccggg cctgcgatga aatccgcaat ggcacgctgt ctgaacattt tgttgtggat
     3241 gtcattcagg gtggcgccgg aacctcgacc aatatgaacg ccaatgaggt tatcgccaac
     3301 cgcgcgctcg aaattatggg gcataaacgc ggtgaatatc aacactgtca cccgaacaat
     3361 catgtgaacc tctcgcagtc taccaatgat gtttatccta ctgctctgaa gatcgcctcg
     3421 gtatgggata tcgagaagct cctcaaggct atggataaac tcaaaggagc attcgaggaa
     3481 aagggcggag aattcgccga tatccttaaa atcggtcgca cacagcttca ggacgcggtt
     3541 cccatgacgc tggggcagga atttgaggcc tacgggataa cgctcggtga aggcgtgaag
     3601 gccctgaagg atgccacgcg agagatttgc gggatcaata tgggcgccac agccattggt
     3661 accggaatca acaccgtcga gggctatgcc gagaaagtcc gtttcctgct ggccgatata
     3721 accggcctcc cgctttacac ggaaccaaat ctggtcgagg ccacctccga tacaggagct
     3781 tttgtacagc tttccggtgt gctcaagcgg gtggcaataa aattatccaa aatctgcaac
     3841 gatctcaggc ttttatcatc ggggccgcgg gccggaatcg gggaaataaa tcttccttcc
     3901 cgccagcccg gatcatcaat aatgcccgga aaggtcaatc cggtcatacc ggaggtggta
     3961 aatcaggtct gttttcaggt aatcggcaat gatgtgactg tcactctggc agccgagggc
     4021 ggtcagctcg agctgaatgt tttcgagcca atcctcggct tcaacatctt ccaatcgata
     4081 gaaatgctgg agcgtgcctg cacagtcttg cgtgagcgat gcatcctcgg gataaccgcc
     4141 aaccgcggga ggtgccagca actggtagaa agttcggtca gcctggtcac cgccctttcc
     4201 ccttttatcg gacatgagaa cgccgccagg attgccagaa aggccctgga gaccgggcgg
     4261 ccggtgcgcg agctggcgct ggaaagcggc ttgattgatg aggaaagatt gaaggatgta
     4321 ctggatccgc gcaaaatgac acatccccgc tccataacgg gttctggcaa aacctgaagc
     4381 aaaaaaaggt gccgccaaaa tggcagtgat tccagaagac ccataagatt attgcggatt
     4441 aaatttgtcg gaaatcaccc ctaaattaat tttcttgcca atttgaatcg gccataccat
     4501 cgtaattctt ataggaagta ccagaaggtc cccagacctg tttctcagtt agtagtatac
     4561 gtgccgaagt tgttgttaga ctttcgagtc tgattgttcg gaacaagata tccaggtgaa
     4621 atgacccctt ttcatggccg gcagaagact tctatctgac ccgggtggtg tgaactgaaa
     4681 gcaaggacag ttgaaggaaa tcatttatga attagatccc caaacagggc cccgaaccct
     4741 gcgatcccaa tgcagacggt catcccgact gcaaatccaa aacaatacta cttatacgga
     4801 atacagcgct cgcccgagcg ctgtattttt atacccgaca tagagcggca tgccctgtcg
     4861 caccattttc gtatatagac tgcaatgctg cttgctttct tagcgccgat ttgataaata
     4921 tacgcaatag caataatatc gggagatact gacatgctca aaggcataca ttttttgtta
     4981 acctataaat gcacttatga atgtgaccat tgctttctat attgcggccc ccatgaaaaa
     5041 ggcacattca cattgaaaaa tgttgaagca gtcctggaac aggccggaga tctgggtacc
     5101 gtaaagacga tctatctgga aggcggagag ccatttctgt tctaccctat gatgctggag
     5161 tcggcgcgca gggctgacga gatgggtttc aaaatcggat ttgttaccaa tgcttactgg
     5221 gcaacatctg aagaggatgc ggaactgtgg ctcaaaccac tgctggagtt aaatatatcc
     5281 gacctgagca ttagcgacga tgaacttcac ggcagtccgg acgcggccgg gaacgccctg
     5341 cgggcagcga aaaaactcgg atttgcctcg ggttcgatct gcattgaaaa accggtaacc
     5401 ctggcaaacc agagtgagtc cgggcaaaaa ggggagccgg tggtcggagg tgatgtgctg
     5461 ttcaagggca gagcggcgga caagttgact gagaatctcc cccggatcag ctacaaaaat
     5521 ttcaaggaat gcccgcatga ggagctcgta tcgcccggac gggtccatgt tgatccatac
     5581 ggtaatgtta tgctctgcca gggcttatgc atcggaaatc tgtttcaaaa gcctctttct
     5641 gacatcatga aggattatga tcacagcaat catccaatat gcgggccgtt attgtccggc
     5701 ggtcccgcac gactggctga aaagtatggc gttgaaattg aatccggcta tgtcgatgcc
     5761 tgccatatgt gttttcttgt gcgccgagca ttattgaagc gatttcctca atttctggct
     5821 cctccgcagg tttatggcac aaccgaaaac gagtgattat cttaatggca tgtaagccca
     5881 tactgccagt aagtatgcag gggttgaacc tgcatctaca tacccaaatt ccaaatttgg
     5941 gcatggatta aattatactt gacaaattga aacaatctat atagttttga ttagaccaac
     6001 gctatgacgg acagcatttt gtgtgtgagt taaaactgta cctatcacct gtactattta
     6061 agtttagaga gttcacccga gaagcagtta ttaagtgtga ctgacgcagc cggttagcat
     6121 gcttctcggg gcttcaggat gggcggctta aaatattttg gaatagtccg aaacaatttc
     6181 taattgaggt attaacagtt gagtcatgaa gggaggtgga tggtcacgat ttcttttccc
     6241 gcacgcatat gagttgtgcg gattttttcc tgtttagagc ctttttaaaa ttccgaaagg
     6301 agcaaatcat gatgagaaaa tttggagtaa ttgtactcct cgcggtattt ctcctaacct
     6361 ctattaccgc tgtagccaag acttccaaca gcagcgctcc tcctggatac aaacgtatct
     6421 atatcgagaa gcctatgtta ttcggggtgg atgataacta ctatgatccc ggtccgagcc
     6481 ttaaattcta ttcgccccca cccgattatt ttccggcagg taactggcgg caggtatatg
     6541 aactggaaac cacccagcat gaccgccagc actacagctc ctctggtcgc cagatcgtct
     6601 atgcgggcta ctggaatgcc ggatgcgatt atatttacat gaactggtcg ggacgcatgg
     6661 tcatcggttc ctcgatcatt cttcaatgca agactgccat atttgactgg gccggcggcg
     6721 tctgggatta tactgccggt ggatcattcc aggccggtcc ttccgggagt ttccagaacc
     6781 tgccgtatac ttttgcgccc aacatcggca taactcccga taatccctgg gctgtttcct
     6841 cgatgagcgt tcgtaacgag gccgaggcat ggaaatccta cattgcttat ggcactgccg
     6901 gctgtccgac ctccagctac ctcactcatc aggccctgcc cggtcctccc aacctggaag
     6961 ggataaccaa cggttattgt gatgagaacc tcggcgacga gacagtgagt ccctatttgt
     7021 ggccgatcat cgacctggat acgattggtg atgttgtaat cacccacctg gtccatcagg
     7081 aatggggttt ctgcgatgca tcacaggccg atgctaataa tattgatgcg gcctccctga
     7141 tctatcagcg caagatcgcc accagtgccg gaagccctga aacgggtacc tgggacggtc
     7201 cccatttcat cgattcagtc tataatattg cctacatcgt ccgtgccgat cacagacccg
     7261 gtaaccataa tgtttacgtt gcctacatga agcccatgta ttaccagtac gacggcgccg
     7321 ggggaagtcc ctgtgccggc accggcgcat accagcttca tcaggatatc gtctatgtgg
     7381 aatccacgga tgatggtgat aactggtcag ccccgattta tgttaccgac tttaaatcag
     7441 gctacgaaga cgacagaact cagccggctg cctacgagat taccggcttt gtcgatccaa
     7501 ccggtgtttt ccatctggtg tggaccgcaa ttaacaatga cgaagcctgt tcattcccgg
     7561 tgacggcaaa actctatcac tgggataatt ccaacaattg tatctcgctg gtttacgacg
     7621 gcagcgagcc ggcgctgttc cccgggtggt cgggcggaat taacgcctgg aacctgatgc
     7681 tggccaaacc caacatctcc tggtgcgacg acaaattgta cgtctcgttt gtcagatacg
     7741 gcgcccatac cactaccaat gacaacggtg agctggttgt tgacgatatc tgcggcacaa
     7801 gttcactggc caatacagat attctggtgg ctgcatcgga tgcaaccggt tcaatgggac
     7861 aggtatggtc cgacgcagtc aatgtaacca atacgattac agatagctgc ctgaccggcg
     7921 aatgcgagtc cgagcactgg gccacgatgg cgatgtattc tactgacagt ctgatgatcg
     7981 aatacatcct cgatctcgag gccggatcca gagcaggtcc taacgatcct aacgagttca
     8041 cccagacctg gacagataac ccggtcatct ttatgacctg gccctgcttt accatggccg
     8101 atctgccgca aaataactgt atttccactt cgccttccac agcgacattt gacgaagtgg
     8161 cgctggcccc tgatggtaac acggctccgc cgtgtaatac cccggcctca gtttcaaaag
     8221 atgttatcgt agccaactgt ggaaacgtaa actacactta ttctgcagcg tcaaatgacg
     8281 gctggctcac gattgatggc ggtgcatccg gttcaattaa cgccggaacc ggtcccaact
     8341 attccgataa tccggcctgg ggcggagccc cagcctgtgc tgcaccggcc actatttcct
     8401 ggaccgccag ttcggcaaca ctgggcgagg gtaattacac cggtactatc tccgttgata
     8461 tagtcgaagc aggtgtggat gatttcgata ttacagtgaa tcttgtggtg acctgcgaat
     8521 actatgtgcc ggaatatgcc agaatcaccg gcggatgctg gagcgtcgat ctgtggaata
     8581 ctccgcaggc cgctgcccag accgatcccg ccggtaatat gcagttctat gcctgcggcg
     8641 gcgattcgat caccccgctt tatcatgaag ccctggcggt cggctggcag gagggcggcg
     8701 taatcaagat gtatagcgat gccgtcacgc aggattccgg ccagctgcac atgcgtgcct
     8761 taagcgatat caccaccagc gaggtcggcg atcccggggc cggcagcggc tactacaaga
     8821 cccagggtta ctggtcgaca cctgacagca gcgtgcaggg catgatcgag tactttgtgc
     8881 ccggccacca agatacatcc gtcctgatcg agaaagtaac tctctggaat accgccggag
     8941 tttctctggg caactttatc ctgggtgagg aatccgactg ggattgtgat ccggattcat
     9001 ccgttgacga aggtcatatc gatcttgatt atgaaatggt ctacatgacc ggcgaccaca
     9061 acggcaccaa tattgctgcc ggcttgaggc ctttcagcgg taccaactct gcgtatggtg
     9121 caagggcgat caacgggtat gattatgcct actacacact gggctggtat cccgataccc
     9181 tgtacaacaa gctgcagtct gtctcgggag ctttcagtct tttcactgat tctgtcaacg
     9241 gcaccgagat gcgaatcctg aacgtcttct atggcggcac cctgggagca aacgacacgc
     9301 tcgagatctg caaggtcaag gccgtcagcc tgacagggga atctggttta cgggatctta
     9361 tggataaggg ctttaccttt atcgagaact atgagattgt acctcccccg ccagactgcc
     9421 agggacgctg cggtgatgcc aacaatgacg gttcggtgaa cgtatccgac gcggtctgga
     9481 tcattaactt cgcctttgta ggcggagatc ctccgcag
//
LOCUS       DSEB01000173            3745 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002488, whole genome shotgun sequence.
ACCESSION   DSEB01000173 DSEB01000000
VERSION     DSEB01000173.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002488"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            93..>3745
                     /locus_tag="ENO07_03165"
     CDS             93..>3745
                     /locus_tag="ENO07_03165"
                     /inference="COORDINATES: protein motif:HMM:PF07705.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00996.1"
                     /translation="MSSRFGLLIILALVIFSPSSICAQIIYGGESYSLEEWDQVAPGL
                     AGEKTALSPVDSIVNNLEIGVVGEIDNHTLSFIQTDSVMYPKSRAIITFPDGFDITTF
                     TKIRYDDNDINSINLVIDTTVISDNTFLIYFADSGSIPVAGSLVGIDMEGITNETESG
                     SYQLILTLTDSTGVVTAGPNFSQPFLLSPGELNSIEVDPSTLQEIYAGDFFQFDCLCS
                     DSFNNALECPEETWYLDDPAGEIDSTGLFLAKYTGFNDFKAKCDAGGITAESGSLYVL
                     AGDPVRFNMDGLPDTTIVAGKILDSSVHVIVEDLYGNEVGRYRGAAWFESSDTAAELL
                     YDSINKYSFNEVDDGQKYFPDSAFIFHTAGQQTIRITDGSISSEYKYIVVKPDIAAHI
                     TVQDPSDTVTAGENFLIQITSLTDVSGNLLSGVVDVQLSGDGIAPDGTEPIINPIFVE
                     AGSGQASQRLPKAGPANFTLTIDTLIYQLDTIRVINSAADHIEFELNTPQTVGQSFAA
                     PASLTVLDRFDNVVSDFNAFEDTVTISPLPPAEGEVINGIIADSQAFVDGVCDLTQFD
                     MYYSGPARFLKFSAVSQSGVQGNSETVEINSALIEQLYLSDSTLYIGDTFSASVTISN
                     FGSDTLTVDTIELFSSQGDLPVQSAIPGPGFRIQGNSSATFELYSFIPETFEIGIIEF
                     GASFIGTYDELTISDTSEFLASMRILSQELADYVPGSLTPVLMTKGGSYSARLELQNL
                     GGAVISLNTDSYLDFILDADTFRTYLEVPTFLPATGTEVVLFFDEIMLPGALASGQYA
                     AALHLFGLQGQSSYSDLVSISDSITLQSPPDLVYLQGSFGPDSAYLGTEIAPVLSVEN
                     LGEAVFEIDRQLSRLELEADGRQVIFRPAVDGDPIFPGGNQISFDSELVPSDSLLTSN
                     TLSLHLEGTANQQNVAFDIEIGPDLLTLLNPGKVQIWSTTNRAPNSPRVNTGQSFNIT
                     VAARNRGQEAVEEIRLSLQGEGSTVLQSSLTIPSLAPDETDSVTFNVTASGTPNIAEV
                     FEASIDNAFGAITGLPAFVENPVDNNTVATVELPASISANLQIVSPPDALDNVLGIKQ
                     DFTLQAEFINSGDAKVSPGTARLILPEGFVTGDLLDSVDFVIGDPLTWDITAPSTPRE
                     TELIVEIVKLPIDSNSLDSALVEKRTDTLAVTVEDELPQTFVDWQFEDYDLVYPGQTL
                     PVVELEFSPGILST"
BASE COUNT          917 a          864 c          939 g         1025 t
ORIGIN      
        1 atataacatc aatatttttg catagctgac agaattattg agataaaatt gtgtgtgtat
       61 taatttaatg tctttaaagg agttgcgctg tattgtcaag tagatttggg ctgctaatca
      121 tcttagcctt ggtaatattc tctccatcga gtatctgcgc acagataatc tatggagggg
      181 aatcatacag tctcgaggag tgggatcagg ttgcccctgg tcttgccggg gagaagaccg
      241 ctctgtcgcc ggttgacagt attgtcaata acttggagat aggagtagta ggtgagatcg
      301 ataaccatac cttgagtttc atacaaacag attcggtgat gtatccaaag agccgagcca
      361 taatcacatt tcctgatggt tttgatatta cgacattcac aaaaataagg tatgatgata
      421 atgatataaa cagcattaat ctggtaattg acacaactgt catttctgat aacactttcc
      481 ttatatattt tgccgattcc ggatctattc cagttgcggg aagcctggtc gggatagata
      541 tggagggaat cacaaacgaa acagaaagcg gatcctatca actgattctg acattaacag
      601 acagcaccgg tgtggttaca gcgggaccta atttcagtca gccttttttg ctctcgccgg
      661 gagagctgaa ttctatagaa gtcgatccct cgacattaca ggaaatctat gccggtgatt
      721 tttttcaatt cgattgttta tgttccgaca gctttaacaa cgcattggag tgtcctgagg
      781 aaacttggta tcttgatgat ccggccggag agatagattc aaccggtttg tttttagcaa
      841 aatatactgg tttcaatgat ttcaaagcaa aatgcgatgc cggtggaatt acggccgaat
      901 cgggttcgct ctatgtgctg gcaggtgatc cggttagatt caatatggac gggcttccgg
      961 atacgacgat tgtggccggt aagatacttg atagctcagt ccatgtcata gtagaggacc
     1021 tgtatggaaa cgaagtagga cgctatagag gtgccgcatg gttcgagagt tccgacactg
     1081 cggccgaact tctatacgat tcgatcaaca agtattcgtt taatgaggtg gatgatgggc
     1141 aaaagtactt ccctgattct gccttcatat tccacaccgc cgggcagcaa acgatccgga
     1201 ttacggatgg gtcgatatcc tctgagtata aatatatagt agttaagccg gacatcgctg
     1261 cccacataac cgtgcaagat ccatccgata ccgttacggc cggagagaat ttcctgatac
     1321 agattaccag cctcactgat gtttccggca atctgctctc aggagtcgtt gacgttcagc
     1381 tttccggaga tggtatcgct cccgatggca cggagccgat cattaatcct atatttgttg
     1441 aagcggggag cgggcaggct tctcagaggc tgccgaaagc agggccagct aatttcactt
     1501 tgactattga tactcttatt tatcagctcg acacaatccg tgtcataaac tccgctgcgg
     1561 atcatatcga atttgagctg aacacaccgc aaactgtcgg tcagtctttt gccgctcccg
     1621 ccagcctgac tgtgctggat cgttttgata atgttgtatc cgattttaat gcatttgaag
     1681 atactgtaac catttctcca cttccaccgg ccgagggtga agtcataaac gggattatcg
     1741 ccgacagcca ggcatttgtg gatggggttt gcgatttgac tcaattcgat atgtattaca
     1801 gtggccccgc ccgcttcctg aaattcagcg ctgtttcaca gagcggagtg cagggaaatt
     1861 cggagactgt cgagatcaat tccgccttga tcgagcagtt gtatttatcc gactcgacgc
     1921 tgtatatagg cgatacgttc tctgcttcgg ttacaatttc caattttggt tccgatacgc
     1981 tcacggttga cacaattgaa ttgttttcat cacagggtga tttgcctgta caaagtgcaa
     2041 ttcccggccc gggatttcgg attcaaggga acagctcagc taccttcgag ctctattctt
     2101 tcatacccga aacttttgaa atcggaataa tagaattcgg ggccagcttc ataggcacgt
     2161 atgacgagct gaccatctcc gacacctccg aatttctggc ctcgatgaga attctcagcc
     2221 aggaactggc cgattatgtg cccgggtcgc taacgcctgt gcttatgacc aagggaggat
     2281 cgtattctgc gcgccttgaa ctgcagaacc ttggcggggc tgtaatcagc ctgaacactg
     2341 acagctatct cgattttatt cttgatgcgg atacattcag gacctatctg gaagtgccca
     2401 cctttttacc ggcaacgggg accgaagtcg ttcttttctt tgatgagata atgcttccag
     2461 gggccctcgc ctccggacag tatgcggcag cgcttcatct tttcggtctt caggggcagt
     2521 ccagttattc ggacctggtg tctatatccg attcgataac cttgcagagt cctccggact
     2581 tggtttatct ccagggctca tttgggcctg attcggctta tcttggaact gaaattgccc
     2641 cggtgttgtc tgttgagaat ctgggtgagg cagtctttga aatcgacagg cagctttccc
     2701 ggttggagtt ggaggctgac ggccggcagg tgattttcag gccggcggtt gacggagatc
     2761 ccatatttcc gggcgggaat caaattagct ttgacagcga acttgtgccg tcagattctc
     2821 tgctcacgtc aaacacatta tctcttcatc ttgaaggcac cgccaaccag caaaatgttg
     2881 cttttgatat tgagattgga cctgatcttc taaccctgct taatcccggc aaggtgcaaa
     2941 tctggagtac aacaaacagg gctccgaatt ctccccgggt caataccgga cagagtttta
     3001 atatcacagt ggcagcgaga aatcggggcc aggaggcggt tgaagagatc cggttgagtc
     3061 tgcaaggaga gggcagcacc gtgttacaga gttcattgac catcccttcg ctggcgcccg
     3121 atgaaactga ttctgttaca tttaatgtaa ccgccagcgg cactccgaac atagcggagg
     3181 tgttcgaggc ttctatagat aacgccttcg gggcaattac cggtctgccg gccttcgtcg
     3241 agaatccggt tgacaataat acggtcgcga ccgtggaact gccggccagc atttcggcca
     3301 accttcagat cgtgtctccg cccgatgctc tggataatgt cctgggcata aaacaggatt
     3361 ttaccctgca agcggaattt ataaacagcg gtgatgcaaa ggtcagccca ggtacagcga
     3421 ggctgattct tcctgaggga tttgtaaccg gcgatcttct cgattctgtt gattttgtaa
     3481 tcggcgatcc gctcacctgg gatattactg cgccgtcaac accgcgagag acagagctga
     3541 tcgtagagat tgttaagctt ccgattgact cgaatagttt agactcagct ctagtagaaa
     3601 aaagaaccga tacgcttgcc gtgaccgtgg aggatgagct gccgcagaca tttgtggact
     3661 ggcaatttga agattacgat ctggtctatc ccggccagac cctgccggtt gtcgagctgg
     3721 aattttcacc cggaatcttg agtac
//
LOCUS       DSEB01000174            2329 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1007229, whole genome shotgun sequence.
ACCESSION   DSEB01000174 DSEB01000000
VERSION     DSEB01000174.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1007229"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1352)
                     /locus_tag="ENO07_03170"
     CDS             complement(<1..1352)
                     /locus_tag="ENO07_03170"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00989.23,HMM:PF08448.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HDS00997.1"
                     /translation="MNKMIVDDLKESTVLEGIPFNAAIVDRDFNVVAANSRFEQYFGP
                     WEGKKCYAAYKGLQSPCKNCPTLETFKDGKKRVIDGVGKDVHGRTAHYVTHIAPIKDN
                     DGKVLYVLEMSHDITESKNWLKEYNILFERVPCYITVIDRDFKIIRANERFREVFGEN
                     VDDHCYKVYKNRSRKCDHCPAAKTFKDGQIHSSNQVGITKTGERAHYVVTTAPLTRGE
                     EEIAHVIEISTDVTQTKKLEEELQRIHAFQKALIKNSNDGILATDTDGNVTIFNPAAR
                     ELLGFPDKGKPSMRDINRMFPGEYIEMMDDKEDKCVLDESIVLDKKGEEIPVRLSGAT
                     LKDRKKTLGYAAFLQDIRELKELEKEKLDAERLSAVGQTVSGLAHTIKNILMGLEGGM
                     YMVHSGLNKDDKERTKEGWGILEKNFDKITSLVKDFLSFAKGRVPNVQMIDPSQLIKD
                     "
     gene            complement(1349..1753)
                     /locus_tag="ENO07_03175"
     CDS             complement(1349..1753)
                     /locus_tag="ENO07_03175"
                     /inference="COORDINATES: protein motif:HMM:PF00072.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDS00998.1"
                     /translation="MAYKVLVIDDEPDVLKYLESVLSDGGYEVKTAESGKDGFEKACE
                     WSPDLICLDIMMPKQSGISVYRQMREDKKTCNIPVIILTGIEKGDDFDFHAWVGDDEL
                     PPPEQFLEKPIKVSAFLETIGKIIGSGKVESK"
     gene            complement(1758..>2329)
                     /locus_tag="ENO07_03180"
     CDS             complement(1758..>2329)
                     /locus_tag="ENO07_03180"
                     /inference="COORDINATES: protein motif:HMM:PF02085.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS00999.1"
                     /translation="PVVKARGGEKLIINGNRNFDLVLFKHKMHEDTLGGKESCVKCHH
                     MNIPGDKESGCWQCHADMNKYTDAFRHDWHASPSGGNLGCVKCHEPDQPKMALTASEC
                     NECHKDLIPPGAAIKVEDYTAPGYVDAMHGSCVECHKEKAAALDKPKLPQCTTCHDQE
                     VSDSINQAIAAKHEGRKSPWVTMPEIEEN"
BASE COUNT          499 a          645 c          516 g          669 t
ORIGIN      
        1 atatctttga taagctgact gggatctatc atctggacat tgggcacccg gcccttggca
       61 aaggagagaa aatcctttac caggctggtt atcttgtcga aattcttttc caggatgccc
      121 cagccttctt tggtgcgctc cttgtcatcc ttattcagtc cggaatgcac catgtacatg
      181 cccccctcca gacccatcag gatgtttttg atcgtgtgcg ccaggcccga aacagtctgg
      241 ccgaccgctg acagacgttc agcgtccagc ttctccttct ccagctcctt cagttcgcgg
      301 atatcctgca gaaatgcggc ataaccgagg gtctttttcc tgtccttcag agtggccccg
      361 gataatctga ccggaatctc ctcccctttt ttatcaagca caattgattc atcgagaaca
      421 catttatcct ctttatcatc catcatctcg atatattcac ccggaaacat gcggttgata
      481 tccctcatcg agggcttgcc cttatcggga aatccaagaa gttcccgggc tgctggatta
      541 aagatggtaa cattgccgtc agtgtcggtt gccagtatac cgtcattgga gttcttgatc
      601 agcgctttct ggaaggcgtg gatacgctgc aattcctctt ccagcttttt ggtctgggta
      661 acatcggtgg agatttcaat tacatgtgcg atctcctcct cgcctcgtgt caggggcgcg
      721 gtggtaacca cataatgcgc ccgttcgccg gtcttggtta taccaacctg gttggaagaa
      781 tggatctggc cgtccttgaa tgttttagcc gccggacagt gatcgcattt gcgggaacgg
      841 ttcttatata ctttgtagca atgatcatct acattttcgc cgaaaacctc gcgaaatctt
      901 tcattggcgc ggatgatttt gaaatccctg tcgatgactg tgatgtagca gggcactctc
      961 tcaaaaagta tattgtattc cttcagccag ttcttgcttt cggtaatatc atgtgacatc
     1021 tccagcacat ataacacctt cccatcatta tcctttatcg gggctatatg ggtgacataa
     1081 tgcgctgtgc ggccgtgaac atccttgccc acgccgtcta tcacacgctt cttgccgtcc
     1141 ttgaatgttt caagtgtggg gcagtttttg catggactct gtaggccctt atatgccgcg
     1201 taacatttct ttccttccca gggtccgaaa tactgctcga agcgtgaatt ggccgccacg
     1261 acattaaagt ccctgtccac aatcgccgca ttaaagggta tgccttcaag aacagtgctc
     1321 tcctttaagt catcaacgat catcttattc atttcgattc aacctttccg gagccgataa
     1381 tcttcccgat cgtttccaga aatgcagaga ctttaatcgg cttctccaga aactgctcgg
     1441 gagggggaag ttcgtcgtcc cccacccatg cgtgaaaatc aaaatcatca cctttttcga
     1501 ttcctgtcag gatgatgacc gggatattgc atgtcttctt atcctcgcgc atctggcggt
     1561 aaaccgatat tcccgactgc ttcggcatca taatatccag gcagattaaa tccggggacc
     1621 attcacaggc cttctcgaaa ccatccttac cgctctcggc agtcttgacc tcatagccgc
     1681 cgtcagataa taccgactcc agatatttga ggacatccgg ttcatcatct atgaccagta
     1741 ctttataagc catctcttca attctcctcg atttcaggca tcgttaccca gggtgatttt
     1801 cttccctcgt gctttgccgc tatagcctga ttgatactgt ccgagacctc ctggtcatga
     1861 caggttgtac attgcggcag cttaggcttg tcgagcgcgg ctgctttctc cttatggcat
     1921 tccacacaac ttccatgcat ggcatccacg taaccgggag cggtataatc ctcgacttta
     1981 atcgccgcac caggcgggat aagatcttta tgacattcat tgcattctga tgcggtcaaa
     2041 gccatcttgg gctgatccgg ttcatggcat ttcacgcacc cgagattgcc ccccgagggt
     2101 gaggcgtgcc agtcatggcg gaaggcatcg gtatatttat tcatatccgc atggcactgc
     2161 cagcatccgc tctccttatc cccggggata ttcatatgat ggcatttcac gcacgactct
     2221 ttgccgccaa gcgtatcctc atgcattttg tgcttgaaca gcaccaggtc aaagttgcgg
     2281 ttgccgttta tgatcagctt ctcgcctccc cgtgccttga ccaccggag
//
LOCUS       DSEB01000175            3745 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002487, whole genome shotgun sequence.
ACCESSION   DSEB01000175 DSEB01000000
VERSION     DSEB01000175.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002487"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            18..938
                     /locus_tag="ENO07_03185"
     CDS             18..938
                     /locus_tag="ENO07_03185"
                     /inference="COORDINATES: protein motif:HMM:PF02016.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDS01000.1"
                     /translation="MNYPKKASHGAKIGIAAVSSMPAKKDVSQGIKNLRKMGYEVFDF
                     TRVLRKNISDLQRIEILHRALSDKSVEVIVMARGGYGSLRILDKIDYRLVNKSRKIMV
                     GFSDFTAISLAVFKKTKLITFSGPMLKTHFAGPVSGLTRESFNDNIHGFIRAGETVNL
                     KQIGGKMLKRGSAPGFLLGGNLSVLCRLIGTEYLPDFTDAILFIEDVDEHAGRLDNAF
                     AQLRNCGILNKVGGVLAGQFSNCFKGSKARQLSEIKALLKDYTDDLNIPVMYDLPFGH
                     ENKIITMPVGLPVRLDSAKGKLTYLETPVE"
     gene            939..1943
                     /locus_tag="ENO07_03190"
     CDS             939..1943
                     /locus_tag="ENO07_03190"
                     /inference="COORDINATES: protein motif:HMM:PF13378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HDS01001.1"
                     /translation="MHIRAELIETNLKETFRISRGESSGKKNCIVHLEDALGECCPSI
                     YYGYSAEDCCQVINESRLEIKEALEFSRQLDYYEEMFSGRKSLLAGLDIVLHDYISRK
                     LDLPLYQYLGIPDPQNLETSYTISIDKPENLEKRLKAAEGFKSVKLKIGSEYDKENLK
                     ILHDRTKFRIRVDANGAFALDQIMELVPIFNDYKLELVEQPLAESSPSDLKMLRKELK
                     VPIFLDESIVEVQDVYKYLGAIDGVNIKLQRVGGIRMALKMIQAARSLGMKIMFGCML
                     ETNIGNCATAHLGGFADFLDLDSSMLLKDDPFEGLSIDRGRIIMPAGKGIGARRKQNV
                     "
     gene            1936..>3745
                     /locus_tag="ENO07_03195"
     CDS             1936..>3745
                     /locus_tag="ENO07_03195"
                     /inference="COORDINATES: protein motif:HMM:PF01520.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDS01002.1"
                     /translation="MFKKIICITIFCVIACAPSSLKTKSDGAPKIEVIYPGKKQQVNA
                     VDSTFILGNVTPGSKLYINNYRIAVHPEGGFLAFLPLVPVDTGWFVFQLRASNDYGDT
                     TLEWPVKLPEPFKTVPLDSLAILSDFNLPARYQELIAGDLLELSIRGTPLAVAQYKIT
                     GLTDWKPMFEISSMIQVPGSETIFGSENLRDSTIGTGVYRAGMFMPSDTALDSADIIF
                     RLCKIVGNSPSDSGLLECVEDTVESILSLKKYDFPQVVELIDSVQTIRHGPRKGYLSI
                     YQPKGVRFIADGKYNNYVRLDLGPGQAAWIPDSSIIYLPEGTPAPYSEVKYIRTFAMD
                     SVTRVSTYLDMMLPYRVEFDPKAGRVGLLVYYVTSDTDWIRYDTDDDLIDYIVWSQPP
                     ANVYRLDIQLNENEIWGYDAFYEDNVLNLDLKKAPKEVVNIEDLKFVIDPGHSRDPGS
                     IGPTGLREADANLAISRKLVQLLRDKGAVVAMTRTGNENVELHERPEIAVRENCDIFI
                     SVHNNALPDGVNPFENHGTSTYYYHMHSKPLAEHIQRKLVDKLEMGSFGHYHANFAVT
                     RPTQYLSVLVECTFMILPDEEARLRGEEFPKKCAEAI"
BASE COUNT          968 a          861 c          988 g          928 t
ORIGIN      
        1 gaaatgcgca gggttatatg aattatccca aaaaggcttc acatggagca aagatcggca
       61 tagcggcggt gagcagcatg cccgccaaaa aggatgtgtc gcagggtatt aagaacctcc
      121 gcaaaatggg ctatgaggtt ttcgatttca ccagagtgct gcgaaaaaac atctctgatt
      181 tgcaaaggat cgaaattctt catcgagctt tatccgataa atcggttgag gtgatagtga
      241 tggctcgagg gggctacggc agtttgcgca ttcttgataa gatcgattac aggctggtca
      301 ataagtcccg caaaatcatg gtcggtttct ccgattttac ggcaatctcg cttgctgttt
      361 tcaaaaagac caaactgata accttcagcg gtccgatgct gaaaactcat tttgccggac
      421 ctgtctcagg actgaccaga gaatcattca acgacaatat tcatggcttc atacgcgccg
      481 gtgagacggt taacctgaag cagatcggcg gcaagatgct caagcgcgga agcgcccccg
      541 gctttcttct gggaggcaat ctctctgtgt tatgtcgtct tatcggtact gaatatcttc
      601 ccgatttcac cgacgcgata ctgtttattg aggatgttga tgaacatgcc ggacggctgg
      661 ataacgcatt tgcgcagtta aggaactgcg gcattctgaa taaagtgggc ggcgttctgg
      721 ccgggcagtt tagcaactgc ttcaagggta gcaaggccag acagctttct gaaataaagg
      781 cactcttgaa ggattatact gatgatctga atattccggt catgtatgat cttcctttcg
      841 ggcacgaaaa taagatcatt accatgccgg tcgggctgcc ggtcaggctt gattcggcca
      901 aaggaaaact tacatatctg gagacaccgg ttgagtaaat gcatattaga gcggaactga
      961 tagagacaaa tttaaaggag accttccgaa tttcaagggg tgaatcctcg gggaaaaaga
     1021 actgtatcgt acacctcgag gatgccctgg gcgaatgctg tccctcgatc tattacggct
     1081 attcagcgga agattgctgc caggtgatca atgaaagcag gcttgaaatc aaggaggcgc
     1141 tggagttttc ccggcagctt gattattatg aagaaatgtt cagcgggcgc aaatcgcttc
     1201 tggccgggct tgatatagtc ctgcatgatt atatctcccg caaactggac ctgccgttat
     1261 atcagtacct gggaattccc gatccccaga acctggaaac atcatacaca atctcaatcg
     1321 acaagcctga aaatctcgaa aagcggctca aggcggctga gggcttcaaa tccgtcaagc
     1381 tcaaaatcgg ttccgagtac gataaggaga acctgaaaat actgcatgat cgcaccaaat
     1441 tcaggatcag ggtcgacgct aacggggcct ttgctctcga tcagatcatg gagctcgtgc
     1501 cgattttcaa tgattacaaa cttgagctgg tggagcagcc cctggctgaa tcatcacctt
     1561 ccgatctgaa aatgttaagg aaggaactga aagtcccgat tttcttagat gagagcattg
     1621 ttgaggttca ggatgtgtat aagtatctgg gcgctataga cggtgtcaat atcaagcttc
     1681 agcgtgtggg cggaattcgc atggcgctga agatgattca ggcggcccgc tcgctgggta
     1741 tgaagattat gttcggatgt atgctggaga ctaacatcgg taactgtgcc accgcacatc
     1801 tgggcggttt tgccgatttt ctcgacctgg acagctctat gctcctcaag gacgatccct
     1861 ttgagggact ctctattgac aggggcagga ttattatgcc ggccgggaag ggtattggag
     1921 cgaggcgaaa gcaaaatgtt taagaaaatt atatgcataa caatcttctg tgtcatagcc
     1981 tgtgcgccct cgagcctgaa gaccaaatcg gacggagcgc cgaagatcga ggtgatatat
     2041 cccggaaaaa aacagcaggt taatgctgtg gactccactt ttatcctggg caatgttaca
     2101 cccggatcaa agttgtatat aaataattat agaattgcgg ttcatccgga gggaggcttt
     2161 ctggcttttc tcccgctcgt ccccgtggat accggctggt ttgtattcca gctccgtgcc
     2221 agcaatgatt acggcgatac cactcttgaa tggccggtca aactgccgga gccgtttaaa
     2281 actgtacctc tggactcact ggcgatattg agcgatttca acctgcccgc gcgttatcag
     2341 gaattgatcg ctggtgactt actggagtta tcaataagag ggacgccgct ggcagtggcg
     2401 caatacaaaa ttaccggcct gactgattgg aagcccatgt ttgagatcag ctccatgatc
     2461 caggtaccgg gcagcgagac aatcttcggc tccgagaacc tgcgggattc gaccatcggc
     2521 acaggggttt accgggctgg tatgttcatg ccgtcagata cagccttaga ctctgcggac
     2581 ataatcttcc ggttgtgtaa aattgtcgga aattcgccat ccgattccgg tttgcttgaa
     2641 tgcgtggagg atacagtgga atcgattctg tcattgaaga aatatgattt tccccaggtg
     2701 gtggaactta tagattcggt gcagacgatt cgtcacggcc cgcgcaaggg ctatctgtcg
     2761 atttatcagc ccaagggagt caggtttatc gctgacggca agtataacaa ttacgtgcgc
     2821 ttggatctgg gaccgggcca ggcggcatgg attccggaca gctcgataat atatctcccc
     2881 gagggcactc ccgcgccata ttcggaggta aaatatattc gtacgtttgc gatggacagc
     2941 gtaacccgtg tctcgaccta tcttgatatg atgctgccct atagagttga attcgatccc
     3001 aaagcgggac gagtcgggct gctggtttat tatgtcacct ctgataccga ctggatcagg
     3061 tatgacaccg atgatgacct gattgattat atagtctgga gtcagccccc ggccaatgtc
     3121 taccgtctcg atatacagct caatgagaac gaaatctggg gctatgatgc tttttatgag
     3181 gacaatgtcc tgaatttgga tttgaagaaa gcgccaaaag aggttgtgaa catagaagat
     3241 ctcaagtttg tgatcgaccc ggggcattca cgggaccccg gctcgatcgg gccgacggga
     3301 cttcgcgagg cggatgccaa tctggccatc agcagaaagc tggttcaact tcttcgtgat
     3361 aaaggcgccg tggtcgccat gacccgtacc ggcaatgaaa acgtggaatt acatgagcgc
     3421 cctgaaattg cggttcgtga aaactgtgac atattcatat cggtccacaa taatgccctg
     3481 cctgacggtg taaatccgtt cgagaatcat ggaacctcga catactacta tcatatgcac
     3541 agcaaacctc tggcggagca tattcagaga aagctggtcg ataaactgga gatgggaagt
     3601 ttcggccatt atcatgccaa tttcgcggtc acccgtccga cacaatatct ttcggtgctg
     3661 gttgaatgta cattcatgat tctgcccgac gaggaagcca ggctcagggg agaggaattt
     3721 cccaaaaaat gcgccgaggc gattc
//
LOCUS       DSEB01000176            5173 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001210, whole genome shotgun sequence.
ACCESSION   DSEB01000176 DSEB01000000
VERSION     DSEB01000176.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5173)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001210"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            678..>5173
                     /locus_tag="ENO07_03200"
     CDS             678..>5173
                     /locus_tag="ENO07_03200"
                     /inference="COORDINATES: protein motif:HMM:PF13229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01003.1"
                     /translation="MKFDKLFQFTVFTLIIALLPGLLTATVLEVTQPVQVTSSNYYER
                     GQAITYDGTYYWLFYGRSATVTGNYQNDNPDVSDYQIYYKKATTVEGLASSTAVAVTG
                     AANTYMGEIGAAYLDGNVWTFGAVPSTNYTDRKSLYGWYSSDGGATWTQVADLADNLP
                     PGAAHHDETAFDGKLYIMANYPDGNTGWHTKYTSDPTASTITWSSYIPLNSTSNLING
                     TGHFFVDGSNLYIGILRTSPTKDNKVLQYNASPESWTELGTASSTGWDATLFKVGSDY
                     VFAQAPYTSDGGGRQYIVAWAGTSPSNVLSGSSHTVVEGKYGTNTWIDMWPIGFTDAG
                     GSSYLFYTSERDDPNQEGTGNIWYLKVDWDVYGDHYSYIQEAVDAITSKSTADTVLLA
                     AGTFTAYDRALAIIDVPMTLMGAGQADDGTGTVLDGGTYGQGVDNTGLGTAWPRAIVV
                     QSDDVVIKNMRVRGYQGDQVTVGGYAIVARAESSWGVTDPTIDNLTLENITFDDCYYG
                     ARESNVVNALVQNTSYEINSGAASYAAYITNSTGTTIRANAYDQGAIWVTDATYGTIG
                     GPNPSDGNVLTNILYNGIWYGQQFAAGTSSEKGLIQNNTVSGAGEGGIVVWNWPGEVA
                     DSIRILGNTVTGAAGGSDDHGGISIRQGSFTNMVISGNSSTNNADNQPGLLISGSVAT
                     SVGISGNVFADNPEDGIKFYNVTRGNVTVNENKITGNASGLVMSLGTTTTLDASANYW
                     GSTDPTTVAGMITGVIDYTPWLGGGTDNAPGFSGDFSELWVDDDSPQSGASNRIQEGV
                     DLVTASTVNIAAGTYDATIDIDTRTDINLVGADKTTTIIKPSATLPWGFAPYYAGRQA
                     ALRVYNSTGINVSGITFDYDLIKGNQIFGFLYWNSEGSLTDNIFKNISVDDASGGYYE
                     MISYLRAPAYTDAARAQISITDNTFMETGRVAIVTHDYVHSVISGNTFYKTTSDFGYA
                     MEIGSQSTAVVSNNTIYGYNTPALSDGSNSAGIYVENSFTAGSPSLTKNVTLSGNEIY
                     DCQWGLYIGNEFDTYAGDVDIVANVTGNNIHDNTDGGVVLTDEDYSAGSSVDVTFQNN
                     TVMSNGNYGYLIFTRGDGDITALLYGETIVDQDTGVYINDYTVGSSVYDVDIYGSSFY
                     NGLNAESNVSGVSWDDGAGVGNCWSDWTTNSGYPTQYNIAGTPGGVDNYPTVDCGLDM
                     TPDEILYLCSGDFTFDIEIGDAVLGLDAANITIRYPADLNFVSATEASGNYQLFYNEF
                     DNATGYDSVQFNFGVLTGSEDGPVTLFSVTMNGSTDYCAGDLIENIYVLLQGHDPLNN
                     IVTIPASPAEPVTVVADCADPVLAVNTADGGYYNVPPVVNFGATDNCDLDAVYYQVDG
                     CDSGGWTAVAVGLSGTAYGPSDWTLPTADWDALSEAQHCISFKIMDDNGRGNADSCSY
                     SWCFTKDVTAPPAPTDLVAQPGHNKVNLSWTNAASDFDHTVIVRNDYVDGGGHGYPEY
                     DDLYT"
BASE COUNT         1322 a         1244 c         1317 g         1290 t
ORIGIN      
        1 atctgtaata ttgtgtaaat catcaggata ccaattgtaa ttccattttt tttgagaaaa
       61 ccggctggaa cttttcgctc tctatctacc gcccgcctcc ataattaaca tcaattctac
      121 ccggattttg ctttctgttc agatgtctat tatttacccc ttaaaatatg gcgaatgttg
      181 tgcattttac ctcgataagc tatccatata aaccggccaa tctttaactt attgtctctt
      241 atagagaaga agtaagctgc caggtcgggt ttttcggcat ggaatttgag atgcagatta
      301 cacagggata cagaactgca aatagcagat ataatcaggt tagttttctc agtaggggat
      361 ggggccttat tgatatccga ccttgatggg caggggagtt ttaccaccca aatacatgca
      421 tagctcaaag agggtctttt ataaactgca ataaaatcat atttgagaga attttcgcat
      481 ttgcggaaaa tctaaaaaga aaatgatctg caatatcgaa gcaatagaag ctaacattga
      541 caagcagcgg agggaggtga tcttctcaaa ttctcagaca gtttaaaaat ttttcaaaca
      601 tcaataacca caaaaccttt ggccgaaggt aaacagggaa agcgcgcaag aatactttcc
      661 ggaaacacgg ggttcaaatg aaatttgaca agttatttca attcactgtc ttcactctta
      721 ttattgctct cctgccaggt ttgcttacag caaccgtatt agaagtcact cagcccgtac
      781 aggtgaccag cagtaattat tacgaaagag gacaggctat cacttacgac ggcacatatt
      841 actggttgtt ctatggcaga tcagccacag taaccggcaa ttatcaaaac gataatccgg
      901 atgtgagtga ttatcaaatc tactacaaga aggccaccac agttgagggc ctcgcttcgt
      961 caactgccgt ggcggttacg ggggcggcaa acacctatat gggtgaaatc ggcgcggcct
     1021 atctggatgg caatgtttgg acattcgggg cggtgccatc gacaaactac acggaccgca
     1081 agagccttta cggctggtac agttcggatg ggggcgccac ctggacccag gttgcggacc
     1141 ttgcggataa ccttccaccg ggcgcggcgc atcatgatga aaccgctttt gacggcaagc
     1201 tttatatcat ggctaattat ccagacggca ataccggatg gcatacaaag tatacctctg
     1261 atccgacggc atcaacgatt acctggagca gctatattcc gctcaattcc acctccaacc
     1321 tgataaacgg gaccggccat ttctttgtcg atggaagcaa cctctatatc ggcatactga
     1381 gaacctctcc caccaaggat aacaaggttt tgcagtataa tgcctcgccc gaatcctgga
     1441 cagaattggg caccgcatcg tcgacaggct gggatgccac gctgtttaag gttggatcgg
     1501 attacgtctt cgcccaggct ccctatacct ccgacggagg cggacgccag tatattgtcg
     1561 cctgggccgg aacgagcccg agcaatgtgc tgagcggatc gtcacacacg gtcgttgaag
     1621 gtaaatacgg taccaacacc tggatcgata tgtggccgat cggctttacc gatgcgggcg
     1681 gaagcagcta tcttttctac acctccgaga gagatgatcc aaatcaggag ggcaccggta
     1741 atatctggta cctgaaggtc gactgggatg tttacgggga tcattattcg tacatccagg
     1801 aagcggtgga tgcgataaca tctaaatcta ctgcagatac tgttttactg gcagccggga
     1861 cattcacagc ctatgaccgt gccctcgcga ttattgatgt tcccatgact ctaatgggcg
     1921 cagggcaggc agatgacggt accggcacag tactggatgg cggaacgtat gggcagggtg
     1981 ttgataatac cggactcggc accgcatggc cgcgtgctat cgtcgtacag tccgatgatg
     2041 tcgtgatcaa aaatatgaga gtcagaggtt atcagggtga ccaggtcacg gtcggcggtt
     2101 acgccattgt ggccagggcc gaatcctcct ggggggtgac cgatccgact atcgacaatc
     2161 tcacgcttga gaacataacc ttcgatgact gctactatgg agctcgcgag agcaatgtcg
     2221 ttaatgctct ggttcagaat accagctatg agatcaattc cggagctgcg tcatacgctg
     2281 catatataac aaattcaacc ggtaccacta tacgagccaa tgcttatgat cagggcgcta
     2341 tctgggtgac cgatgcgacc tatggtacca tcggtggacc gaatccatct gatggaaatg
     2401 tgctgacgaa tatcctttat aatggtatct ggtatggtca gcaattcgca gcaggaacat
     2461 catctgaaaa gggtctgatt cagaataaca cggtctcggg cgcaggagaa ggcggaatag
     2521 tcgtatggaa ctggcccggg gaagtcgccg atagcattcg aattcttggt aatactgtga
     2581 ccggcgccgc aggcggttca gatgatcacg gcggcataag catccgccag ggcagtttca
     2641 ccaatatggt tatcagcggc aacagctcaa ccaataatgc cgacaatcag cccggtctcc
     2701 ttatttcagg aagcgtggcc acgagtgttg gtattagcgg taatgtattc gcagataatc
     2761 cggaggatgg tattaagttc tataatgtca ctcgtggaaa tgtgactgtc aatgaaaata
     2821 agatcaccgg taatgcttcg gggctggtga tgagcctcgg cacaacaacc accctggacg
     2881 cctccgcaaa ctactggggc agtactgatc caaccacagt tgcgggtatg attacgggtg
     2941 taatcgacta taccccctgg ctgggcggtg gaacggataa tgctcccggc ttcagcggag
     3001 atttctccga actgtgggtc gatgacgaca gtccgcagtc cggtgcaagc aaccggattc
     3061 aggaaggcgt cgatctggtg acggccagca cagtcaatat cgcggcggga acctatgatg
     3121 ccaccattga tatcgatacc cgtaccgata tcaacctggt tggcgctgac aagaccacga
     3181 cgattatcaa gccgagcgcg actctgccct ggggctttgc accctattac gcgggacgtc
     3241 aggcggcatt gagagtttac aattccaccg ggatcaatgt cagcggcatt acattcgatt
     3301 acgatctcat aaaaggtaat cagatcttcg gtttcctgta ctggaactcg gagggaagtc
     3361 tgactgacaa catattcaag aacatttcgg tcgatgatgc ctccggcgga tactatgaga
     3421 tgatttcgta tctgcgagcg cccgcatata ccgatgccgc tcgtgcgcag atttcgatta
     3481 cggataatac ctttatggag acgggcaggg tggcaatcgt aactcacgat tatgtgcact
     3541 ctgttatcag cgggaatacg ttctacaaga ctacaagcga tttcggatat gccatggaga
     3601 ttggttcgca gtcaaccgcc gttgtcagca ataataccat ctatggctat aacaccccgg
     3661 ccttatctga cggcagcaac tcagccggaa tatatgtcga gaattccttc accgcaggca
     3721 gtccgtcttt aaccaagaat gtgaccctct ccggtaatga aatctatgac tgccagtggg
     3781 ggctctatat cggaaacgag ttcgacactt atgctggtga tgtagatatc gttgcaaatg
     3841 tgaccggaaa taatattcat gacaatactg atggcggcgt tgtcctcacc gatgaagatt
     3901 attccgccgg ttcatcggtt gatgttacct tccagaataa taccgtgatg agcaatggaa
     3961 attacggtta cctgatcttc acccggggtg acggtgatat taccgcactc ctctatggcg
     4021 agaccatagt agaccaggat accggtgtct atattaatga ctacacggtc ggttcgagtg
     4081 tatatgatgt cgatatttac ggcagctcat tttataatgg cctgaacgcc gagtccaatg
     4141 tcagcggtgt atcgtgggat gacggcgctg gagtcggaaa ctgctggtca gactggacca
     4201 ccaacagcgg ctatccaact caatacaata ttgccggcac tcccggaggt gtcgataact
     4261 atcctaccgt cgattgcggt ctggatatga ctcctgatga gatcctctat ctttgcagcg
     4321 gggacttcac cttcgatatt gaaataggcg atgctgttct gggtttggat gctgccaaca
     4381 tcactattcg ttatcccgcc gatcttaact tcgtgagcgc aactgaagcg tcaggtaatt
     4441 atcagctctt ctataatgag ttcgataatg ctaccgggta cgacagtgtg cagttcaact
     4501 tcggcgtgct cacaggctct gaggacggcc cggtgacctt gttcagcgta actatgaatg
     4561 gttctaccga ttactgcgcc ggtgatctga tcgagaatat ctatgttcta ttacagggac
     4621 atgatccctt gaataatatt gtaactattc ctgcttcccc tgctgagccg gtaacggttg
     4681 ttgcagattg cgccgaccct gttctggctg taaataccgc cgacggtgga tattataatg
     4741 ttccgcccgt ggttaacttc ggcgccaccg acaattgcga tcttgacgcg gtctattatc
     4801 aggtggatgg atgcgattcg ggcggatgga cggcagtcgc tgtcggattg tccggtaccg
     4861 cctatggccc gtcggactgg accctgccga cagcggactg ggacgccttg agcgaggccc
     4921 agcattgcat cagcttcaag attatggatg ataatggacg cggtaacgct gattcctgtt
     4981 cttatagctg gtgttttacc aaggatgtaa ccgctccgcc cgcgccaact gatctggttg
     5041 ctcagcccgg acataataag gtcaatctga gctggacaaa tgccgcctct gatttcgatc
     5101 acacagtcat agtaagaaac gactatgttg acggcggcgg gcatggctat ccggaatatg
     5161 atgatctcta cac
//
LOCUS       DSEB01000177           11817 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000205, whole genome shotgun sequence.
ACCESSION   DSEB01000177 DSEB01000000
VERSION     DSEB01000177.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11817)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11817)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11817
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000205"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            627..983
                     /locus_tag="ENO07_03205"
     CDS             627..983
                     /locus_tag="ENO07_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HDS01004.1"
                     /translation="MLDKYLTVAVFLFGTVGFVLVTLFVAKLLRPARPSEIKLQNYEC
                     GESPVGSSWVQFNVRFYIFALLFVIFDVETVFIFPWAVVYDTLGWFGFIEMTIFLAIL
                     IYGLVYAWKKGVLKWV"
     gene            974..1495
                     /locus_tag="ENO07_03210"
     CDS             974..1495
                     /locus_tag="ENO07_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013408089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HDS01005.1"
                     /translation="MGVASKIPVYLEKAPGGGILVTSIDKILNWSRASSLWYMLFGTA
                     CCAIEMMCTGASRYDFDRLGMIFRASPRQSDLMFVAGTITHKMASRVKILYEQMAEPR
                     YVIAMGGCAVRGGPFYYDSYAVLKGIDKVIPVDVYIPGCPPRPESLMEGCLKLREKIR
                     KEKMADWSSDSAK"
     gene            1485..1961
                     /locus_tag="ENO07_03215"
     CDS             1485..1961
                     /locus_tag="ENO07_03215"
                     /inference="COORDINATES: protein motif:HMM:PF00329.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HDS01006.1"
                     /translation="MQNEQLVSQLVEKFGAQAEKVELNPAQPALVVDKSILVEFLKQI
                     KEDENFKIDYLSCISAVDYLENGMEMNYHLCSIELRYKLTVKVKLDREKPEVPTIREI
                     HPGADWHEREAWELFGIDIKGHPNLKTLLLPEDWKDGWPMRRDWEGNDDFVKMPEF"
     gene            1966..3129
                     /locus_tag="ENO07_03220"
     CDS             1966..3129
                     /locus_tag="ENO07_03220"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003192527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HDS01007.1"
                     /translation="MDEKELHIGEHQEQDEQNPVARELHTEEFLVNMGPQHPSTHGVC
                     RLLLTMDGEVIVKVEPYIGYLHRAIEKICENRIYNQITPFMDRFEYVSSMLADWVYAL
                     CVERIGQIEVSPRAEYLRVIFGELQRIASHLIYWGVFAMDLGAVTPFLYGLREREMIM
                     DLFEETCGQRLTYNYIRIGGVSQDINPGFEQKCKKFTSYMCQVLEDYETLLNENPIFL
                     SRTKNIGVAPPELSVAYGVSGPSLRASGVNWDIRKDDPYGYYEKFEFEIPQGKNYDTW
                     DRYQARVIEMRESCKIIEQALDGLPEGDPTFKMRASFKLPEGEYYARIESSRGEMGCY
                     IVSKGEKKPYRLKLRGSSFNNLMIIPEISKGLKIADLVAIFASYDIIMPEVDR"
     gene            3129..4118
                     /gene="nuoH"
                     /locus_tag="ENO07_03225"
     CDS             3129..4118
                     /gene="nuoH"
                     /locus_tag="ENO07_03225"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HDS01008.1"
                     /translation="MFLGIELPQIVLSAIYFALTLGVVLLMALFLVWWERKVSAHIQV
                     RFGPMMTGKWHGWSQTLADALKLLQKEDIVPRAADKGVYFWAPVIVFGATMMTFVVIP
                     WSPRFIAADLNVGILYIIAITTFTIISLLMAGWGSNNKYSLLGGMRSAAQIVSYEVPL
                     TLSIMGVVLLAQSLSMQDIVADQNKFWNWNIIRQPLGFLVYLVAATAEVNRTPFDLPE
                     AEQELVAGFNVEYSGMKFAMFFLAEFVNMFVVAAIATALFLGGWWGPFGNESLGWLWF
                     VLKSMGVVFVLMWFRWTYPRLRVDHLMEFAWKWLLPLAFLNLLATGLIYNLVK"
     gene            4122..4844
                     /locus_tag="ENO07_03230"
     CDS             4122..4844
                     /locus_tag="ENO07_03230"
                     /inference="COORDINATES: protein motif:HMM:PF13187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDS01009.1"
                     /translation="MGMVKDFFSGIYNLVVGLFVTGKHLGRHAITLQYPKERWEMPER
                     SRGVVVLLSDPETGELNCTACLLCMKACPTGAIIIDREKNPETKRWFPTKFVIDNTIC
                     AFCGLCEESCNFDAIKLTGKYEFSVFDQSELIYDMDRLAELGRDVKYTPRKKPAAKKA
                     AEQKKPPAEGKKPDEKKEDTAEKKPEEKPDKIETNPAEKKEEKAEASDKKTEDQESRK
                     PEEAKQGDLGLDKEEDKKESGN"
     gene            4844..5359
                     /locus_tag="ENO07_03235"
     CDS             4844..5359
                     /locus_tag="ENO07_03235"
                     /inference="COORDINATES: protein motif:HMM:PF00499.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HDS01010.1"
                     /translation="MLEGSTAIAFYIIAFVVILSAIAVVTLKNIFHAALFLVLTLFAV
                     AALYILLSAEFLAAVQVLIYVGAISILMIFAVMLTAQLTNYNIKQTNEQSLPALLISI
                     CFVAVTIFAIATNHKLGGFPLAESTEVAGSNTAHLGQLLMRDFILPFEVVSVLLLAAL
                     IGAIVIAKRDK"
     gene            5363..5674
                     /gene="nuoK"
                     /locus_tag="ENO07_03240"
     CDS             5363..5674
                     /gene="nuoK"
                     /locus_tag="ENO07_03240"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HDS01011.1"
                     /translation="MQVGLVHFLSLAAILFGIGLYGVLTRRNAIGILMCIELMFNAVN
                     INFITFAKFITPEKLTGQVFAVFVIVIAAAEATVGLAIVILIYRNFKDVFIDRMNIMK
                     W"
     gene            5678..7615
                     /locus_tag="ENO07_03245"
     CDS             5678..7615
                     /locus_tag="ENO07_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HDS01012.1"
                     /translation="MVDHLWTVPAMPIVAFAVIVLFTRFSEKLSKTISIFAIAYGFVY
                     STITLFQTLGNPAGFVIDKGFDWLVLGSFTLQIGMYVDGLTVVMLMVVTIVALLVHIY
                     SIGYMKGDPRTSRYFAFLSLFSFSMLGLVLANNFFLIFCFWELVGVSSYFLIGFWYEK
                     KSASDAGKKAFITNRIGDYGFIIGLLLTFFTFGSLAYSDIFGAVHHDPSHFSAGTLAA
                     IGIFLFCGAVGKSAQYPLHVWLPDAMEGPTPVSALIHAATMVAAGVYMVARALAIYHA
                     APEAGMVVAWVGGITSLMAATIGLVQKDIKRVLAYSTVSQLGYMMMALGLGSFAAGTF
                     HLMTHAFFKACLFLGAGSVIHAVHAQDIFRMGGLSRKMKATSTTFIIASLSISGIFPL
                     SGFWSKDEIIASAEGYLPLLILAIGVAFMTAFYMFRLVFLTFFGEPRDKDAFEHAHES
                     PKVMTWPLIVLAFLSIFAGWVGIPWLPKGFSSFAIDFGHGVHHAEFNFLLAGISTVVA
                     LSGIFLAYMIYIRKAIDHVAIGQKFSLAYKFLFNKWYWDEIYDAIVIRPILWFAEFLA
                     KFDKYVIDGLVNFMGKFTLVLSWLHNLFDKYVIDGAVNGTGYTVKGIGWIISRAQTGR
                     LYNYAFIIVLGVVVILIVKLF"
     gene            7647..9206
                     /locus_tag="ENO07_03250"
     CDS             7647..9206
                     /locus_tag="ENO07_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HDS01013.1"
                     /translation="MESFSFPILTLMIFIPIIGMLILAFLKQSQLLAIRTVSTVFSGI
                     PLVLAVILLANYDPGGGFQFVEKVSWIPALNIHYFLGADGISVPMLMLTALLSFISII
                     ASFGITHRAKEYFVFFLLLEVGMMGVFAALDFFLFYVFWEIMLVPMYFLIGIWGGPRK
                     IYAAIKFFLYTLFGSIFMLVGILILYFSSVDPSTGAHIYTLDLTILAQMAQNGNGLFL
                     REGLQLLVWMFFFIAFAIKVPVWPFHTWLPDAHVEAPTAVSVILAGVLLKMGIYGLLR
                     ISFPILPYGTDYFSTFLAVLGVIGIIYGALVSMAQKDLKKLVAYSSVSHMGYCLLGMS
                     VFSHFGQEFIVGISGAMFQMISHGLITGALFLLVGVIYDRAHTREIAAFGGLWTKVPV
                     YGSLMTFFCMASLGLPGLSGFISEFLVFVGGFAKYKVLTGIAVLGVVLTAGYFLRMIQ
                     KVFLGQFNIKWSDLTEINFREIFTVTPLAILTLLIGIYPKVLSYMIDDTLGDLSNSII
                     NGVNIVKGILG"
     gene            9203..10663
                     /locus_tag="ENO07_03255"
     CDS             9203..10663
                     /locus_tag="ENO07_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HDS01014.1"
                     /translation="MNIRLDLMIPELFLFCWAIVVLTADLFFIKEKKNILLYMSQFGF
                     LAAAALVIFTPTGPYEMAFKWMFINDPFASFFKIVILLAGFMAVSSSYEAVQKSIEHK
                     GEFFGLVIMSAVGMMFLVSSGELVSLYVALELSTIPLFILTAFQKRYLKSSEAGLKYL
                     ILGAFSSALLLYGISFMYGMTGTTVLIAGSINILQYSNSMHFGLVMALALVVAGLGFK
                     LALVPFHMWAPDVYEGAPTPITSYLSVASKAAGLGALVRIIYGFFTVGTASDYWGLLI
                     AILAAMAMIVGNVAAILQSNIKRMLAYSSIAQAGYILVGFVAASQLGLSGISIYIFAY
                     LFANMGAFAVVVAVGHKLNSDKIEDYAGMARRSPLIAGSMTVFLLSLAGIPPTAGFIA
                     KYKVFMAAIENGTLLWLVMIAVATTVVSIFYYARVIKEMFITEREVAANPEGRWILSS
                     PITIAILVGLLGTLIIGIFPQPFLELASTVTQSFVY"
     gene            10978..>11817
                     /locus_tag="ENO07_03260"
     CDS             10978..>11817
                     /locus_tag="ENO07_03260"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00359.20,HMM:PF00571.26"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDS01015.1"
                     /translation="MRLSRILDKDLVVPRLKSRYKLDAIGEILDRVIQKYPHLDRQTL
                     EAKIIERENIENTSYGRGFAFPHARTDAVDEMYTALGLAPEGLEDRTKDQQKLQVICL
                     MLTPSTISKSYLQTLSAFASFARTPGNTERLLSSRTVDEIIDVIEESGVEVKRELSVG
                     DVMKRDVITVKPQDSLKTVANVMFKHRISGVIVVDDENHVVGMISNRDIIRAALPDYQ
                     SLISNLAMSYDSESFENILRTEENVRVGDLMDKNVETVTEDTSVVETAALMLFKDLRR
                     APII"
BASE COUNT         2789 a         2828 c         2972 g         3228 t
ORIGIN      
        1 cctgttaata cagcttctta attatcaccg aattctccgt gaccggcaac gaattgtaaa
       61 gatatataat aatgaagata gaattttcat ccgcttctat gattttggaa atgatctgat
      121 aaacttcaac cttctaacgg ttgaaaacca gcccggcgtg tgcgactata ccatgagttc
      181 ggtgcccttg cggggatgga agacgcatga gatcacggac attttaaaga aaaagagttt
      241 tgtttcaatc cgcaaacagg gggatatcaa gggatcgaaa ttcgatagaa ccgaatcgaa
      301 tgatcttgtc ctgctggcac gcaggagaca acctgtatat cattcaggaa gacacttgaa
      361 tcactgaatg aaggcataat cccggctgac cccacaccaa atccaaatta cataaagagg
      421 aggattcaat cctgtatatt aaatcctcta tttttttaaa gatattatta ccgttttcgt
      481 atcactcaag agatggaatt ccataagcaa atttattcca gaattcgctt gacaaaattc
      541 gcgaaaagtc ttttatgtaa tacttatttt tgtgaagaac gtcacaaagc tctgatggca
      601 gaacggcgct ttgtggggag gtcactttgt tagataaata tcttaccgtc gcggtttttc
      661 tttttggcac agtcggcttt gttctcgtaa ctctttttgt ggcaaaacta ttgcgacccg
      721 cccggccctc ggaaatcaaa ctccagaact atgaatgcgg tgaatcccct gtcggatcgt
      781 catgggtgca gttcaatgtg cgcttctata tttttgcact tctgtttgtc atttttgatg
      841 ttgagaccgt attcattttc ccatgggcgg tcgtatatga caccctggga tggttcggtt
      901 ttattgagat gacaattttc ctggcgatac tgatttacgg cctggtttat gcctggaaga
      961 aaggtgtttt gaaatgggtg tagcatcaaa aattcctgta tacctggaga aggccccggg
     1021 cggcggcata ctggtgacct caatcgacaa gattctaaat tggtcccgcg catcatcgct
     1081 ctggtacatg ctgttcggta ccgcctgttg cgcgatcgag atgatgtgta cgggcgcctc
     1141 cagatatgat ttcgatagat tgggaatgat cttccgcgcc tcgccccgtc agtctgatct
     1201 gatgtttgtg gcgggcacga tcacacataa aatggcttcc cgtgtaaaaa ttctatatga
     1261 gcagatggcg gagcctcgct atgtaatcgc aatgggcgga tgtgctgtcc gcggcggccc
     1321 cttttattac gattcctacg ctgtgctcaa gggcatagac aaggtcattc cggtggatgt
     1381 ttatatcccc gggtgtcccc cgcgtccgga atcgttgatg gaaggctgcc tgaagctgag
     1441 agaaaagatc agaaaagaaa aaatggccga ctggagttcc gacagtgcaa aatgagcaac
     1501 tggtctcgca acttgtagaa aaatttggcg ctcaagcgga aaaggtggaa ttgaatcccg
     1561 cccagcccgc cctggttgtg gataaatcca tcctggtcga gttccttaaa caaatcaagg
     1621 aagatgaaaa tttcaagatc gactatctct cctgcatctc ggccgtcgat tatctcgaaa
     1681 acggcatgga gatgaactat catctctgtt ccatcgaact gcgctacaaa ttgaccgtaa
     1741 aggttaaact cgacagagaa aagccggaag ttcccacaat cagggaaatt caccccggag
     1801 ccgactggca cgagcgcgag gcatgggagc tttttggaat agatataaag ggtcatccca
     1861 atttgaaaac gctgctcctc ccggaggact ggaaggatgg atggccgatg cgccgggatt
     1921 gggaaggcaa tgatgatttt gttaaaatgc ccgagtttta attaaatgga cgaaaaagag
     1981 ctgcacatag gagagcatca agaacaggac gagcagaatc ctgtggcccg ggaacttcat
     2041 accgaggagt ttctggtcaa tatgggaccg cagcatccct ccacgcatgg tgtctgccgg
     2101 ctgcttctga ccatggacgg cgaggttatc gtcaaggtcg aaccttatat cggctatctg
     2161 catcgagcca ttgaaaaaat ctgcgaaaat cgcatctata accagatcac ccccttcatg
     2221 gacaggttcg aatatgtctc ctccatgctg gcggactggg tttatgctct gtgcgtggag
     2281 agaatcggac agatcgaagt ctcgccccgc gcggaatatc tgcgcgtaat tttcggtgag
     2341 cttcagagaa tagcctcgca tctgatctac tggggcgttt tcgcaatgga tctgggagcc
     2401 gttacgccgt ttctttacgg tctccgcgag cgcgaaatga ttatggattt gttcgaggaa
     2461 acctgtggac agagattaac ttacaattat atccgtatcg ggggagtctc ccaggatata
     2521 aatcccggct ttgagcagaa atgcaaaaaa ttcacgagct atatgtgtca ggtgctcgaa
     2581 gattatgaga cgctcctgaa cgagaatccg atctttctca gtcgcaccaa aaatatcggg
     2641 gtggcccccc cggagctgtc tgttgcttac ggtgtctccg gcccctccct gagggcttcg
     2701 ggcgtgaatt gggatatccg caaagatgat ccctacggtt attacgaaaa attcgaattc
     2761 gagatacccc agggcaagaa ttatgacacc tgggaccgtt accaggccag agttatcgag
     2821 atgcgcgagt cctgtaaaat cattgagcag gctttggacg gcctcccgga gggcgatccc
     2881 acattcaaaa tgcgggccag tttcaaactg cccgagggcg aatattacgc ccgtatcgaa
     2941 tcctcacggg gagaaatggg ctgctatatc gtttcgaagg gagaaaagaa accctaccgc
     3001 ctgaaactgc gcggttcatc attcaataat ctgatgatta taccggaaat ttccaaaggg
     3061 cttaaaattg cagacctggt cgccatcttc gcctcatacg acattataat gccggaggtc
     3121 gacaggtaat gtttcttggt atcgagctgc cccagatcgt gctttctgcc atctatttcg
     3181 cgttgaccct gggggtcgtt ttgctgatgg ctcttttcct ggtctggtgg gagcgcaagg
     3241 tctcggcaca tattcaggtt cgattcgggc cgatgatgac cggcaaatgg cacggctggt
     3301 cgcagaccct ggcggatgcc ctcaagcttc tgcaaaaaga agatattgtg ccacgtgccg
     3361 ctgacaaggg cgtatatttc tgggccccgg tgattgtctt cggcgccacc atgatgactt
     3421 tcgtggtcat accatggtcc cccagattta tcgccgccga tctcaacgtt ggaatccttt
     3481 acattatagc gatcacgacc tttacgatca tcagcctgct catggccggc tggggatcga
     3541 ataataaata ttcactcctg ggcggtatgc gttctgcggc ccagatagta tcttacgagg
     3601 tacccctgac gctttcaatt atgggcgtgg tgcttctggc ccagtcactt tcgatgcagg
     3661 atatcgtggc cgatcagaat aaattctgga actggaatat aatccgccag cccctgggat
     3721 ttcttgtcta tctggtggcc gccaccgcag aagtgaaccg tacacctttc gacctgcctg
     3781 aagccgaaca ggagcttgtg gctggtttta acgtcgagta ttccggtatg aaattcgcca
     3841 tgttcttcct ggctgaattt gtaaatatgt tcgtggttgc cgccattgcc accgccctct
     3901 tcctgggagg ctggtgggga cctttcggca acgaatccct gggatggctg tggtttgtat
     3961 taaagagcat gggcgtggtt ttcgtattaa tgtggttcag gtggacctac ccgcgattga
     4021 gggttgacca tctgatggag tttgcatgga aatggctgtt gccgctggcc ttcttgaacc
     4081 tcctggcaac tggcttaata tataatttgg ttaaataagc aatgggcatg gtaaaagact
     4141 ttttctcagg catatacaat cttgtggtcg gcctttttgt caccggcaag catctgggga
     4201 ggcacgcaat cacattgcag taccccaagg aacgctggga gatgcccgaa cgttcccgcg
     4261 gcgtggtagt acttttatcc gatccagaga ccggcgagct gaattgcacc gcctgtctgc
     4321 tctgcatgaa agcctgcccg accggggcaa tcattataga cagggagaag aacccggaaa
     4381 cgaaacgctg gtttccgaca aaattcgtta tagataatac catatgcgcc ttctgcgggc
     4441 tgtgcgagga atcctgcaat ttcgacgcaa tcaagctgac cggcaaatac gaattttctg
     4501 ttttcgatca gtccgagctc atatatgaca tggatagact ggccgagctt ggccgggatg
     4561 tgaaatatac cccgcgcaag aaacccgcag ccaaaaaagc agccgagcag aagaagccgc
     4621 ctgcagaggg caaaaagccg gacgagaaga aagaagatac ggctgagaaa aagccggaag
     4681 aaaaaccgga taagattgaa acgaatccgg cagagaaaaa agaagaaaaa gctgaagcaa
     4741 gtgataaaaa gactgaggat caagaatcca gaaagcctga agaagccaaa cagggagatt
     4801 tgggcctgga taaggaagag gacaaaaagg aatcggggaa ctgatgctgg aaggatcaac
     4861 ggcaattgca ttctatataa tcgccttcgt ggtgatcctg tctgccatag cggttgtcac
     4921 acttaagaat atttttcatg cggccctgtt cctggtgctg actctttttg ctgtagccgc
     4981 attgtatatt ttgttatcgg ccgaattttt ggccgccgta caggtcctga tctatgtcgg
     5041 tgcgatctcg attttgatga tattcgcggt catgctgacc gcacagctca caaattataa
     5101 tatcaagcag actaacgaac aatcgctgcc cgcgcttctg atatcgatat gctttgtagc
     5161 cgtaaccatc tttgccattg caacaaatca taaactggga ggattcccgc tggcggaaag
     5221 taccgaggtt gcgggttcca ataccgccca cctggggcag cttctgatgc gggatttcat
     5281 actgcctttt gaggtggttt ctgtcctttt gctggcggca ttgataggcg cgattgtgat
     5341 tgccaagagg gataaataga aaatgcaagt gggattagta cactttctgt cactggccgc
     5401 aatccttttc gggattggac tgtacggcgt tttgacccgc cgcaatgcga tcggaattct
     5461 gatgtgtatt gagctgatgt ttaatgcagt caatattaat ttcatcacct ttgccaaatt
     5521 catcactccc gagaaactca ccggccaggt cttcgccgtg ttcgtaattg tcatagcggc
     5581 cgccgaggcc accgttgggc tggctattgt aattttgata tacaggaatt ttaaggatgt
     5641 gttcattgac cgaatgaaca tcatgaaatg gtaatttatg gttgatcatt tgtggacagt
     5701 accggcaatg ccgattgtgg catttgcagt catagtgtta ttcacgcggt tttcggaaaa
     5761 actatccaaa accatctcta ttttcgccat cgcctacggt ttcgtctatt ccactatcac
     5821 attgttccag accctgggaa atccggccgg ttttgtaatc gacaaaggct tcgactggct
     5881 cgtattgggc agctttacgc tgcagatcgg aatgtatgtt gatggcctga ctgtggtcat
     5941 gctgatggtt gttaccattg tagccctgct ggtgcatata tactcaatcg gctatatgaa
     6001 aggcgaccca aggacctcgc gctatttcgc attcctgtcg cttttctcat tttcgatgct
     6061 gggtcttgta ctggccaata atttcttcct gatcttctgc ttttgggagc tggtaggtgt
     6121 gagctcctac ttcctgatcg gtttctggta cgagaaaaaa tcagcatcag atgccggcaa
     6181 aaaagcattc attacaaacc gtatcggaga ttacggcttt ataatcgggc tccttttaac
     6241 cttctttaca ttcgggagtc tggcatacag tgatattttc ggcgccgttc atcatgatcc
     6301 ctctcatttc tcggctggaa ctctggccgc gatcggaatt ttcctcttct gcggcgcggt
     6361 cggcaaatca gcccagtatc ctctgcatgt ctggctgccc gacgccatgg aaggtcctac
     6421 tccggtttcg gctctgatcc atgccgccac gatggtcgcg gctggtgttt acatggttgc
     6481 ccgggcgctg gccatatacc acgcggctcc tgaggccgga atggtggttg catgggtggg
     6541 gggcataacc tccttgatgg ccgctaccat cgggctggtg caaaaagaca taaagcgtgt
     6601 tctggcctac tcgaccgtct cacagctggg atatatgatg atggccctcg ggctgggttc
     6661 attcgcagcg ggaacattcc atctgatgac ccatgctttc ttcaaggcct gtctcttttt
     6721 gggcgccggt tctgtgattc atgctgtaca tgcccaggac atcttccgca tgggcgggct
     6781 gtccagaaaa atgaaagcaa catcgacaac atttattata gcgtcacttt cgatttcagg
     6841 aatcttcccg ctctcgggat tctggtccaa agatgagatt atcgcctcgg cagaaggcta
     6901 tctgccgctt ctgattctgg caattggggt ggcctttatg acggctttct atatgttcag
     6961 gctggtcttt ttgaccttct tcggcgaacc ccgcgacaaa gatgcctttg aacatgcgca
     7021 tgaatcgccc aaggttatga cctggcccct gatcgttctt gccttcctct cgatcttcgc
     7081 gggatgggtg gggattccct ggctgcccaa aggattcagc tcttttgcaa tcgacttcgg
     7141 ccacggtgtc catcatgccg aattcaattt ccttctggcg ggaatctcga cggtggtggc
     7201 cttaagcggg attttcctgg cctacatgat atatatcaga aaagcaattg accatgtggc
     7261 catcggccaa aaattcagcc tggcctacaa gttcctgttc aataaatggt attgggatga
     7321 aatctatgac gccattgtaa ttcggccgat tttatggttc gccgaattcc tggcaaaatt
     7381 tgataaatat gttatcgacg gccttgtcaa cttcatgggc aagttcacgc ttgtcctttc
     7441 ctggctgcat aacctgttcg ataagtacgt gatcgacggc gctgtgaatg gcacaggtta
     7501 caccgtcaaa ggcatcggat ggatcattag ccgggcccag acaggccgcc tgtataacta
     7561 tgcttttatc atagttcttg gagttgtggt aatcttgata gttaagcttt tctaataaag
     7621 gaagctaaga ggaggagtgc atagcgatgg aatctttttc gttcccgatt ttaacgctga
     7681 tgatcttcat tccaataatc ggaatgctga tcctggcgtt tctgaaacag agccagctgc
     7741 tggcgatcag gacggtgtcc accgtcttct ccggcattcc actggtattg gcagtaatcc
     7801 tgcttgcaaa ttatgatccg ggcggcggct ttcagttcgt tgaaaaggtc tcctggattc
     7861 cggcattaaa tattcactat ttcctcggag ccgatggtat ttcggtaccg atgctgatgt
     7921 tgaccgcact cctctcattc atatcaataa tagcatcgtt cgggataacc catcgcgcca
     7981 aagagtactt tgttttcttc cttctgctgg aagtcgggat gatgggcgtt tttgcggccc
     8041 tcgacttctt cctcttctat gttttctggg agatcatgct ggtaccgatg tacttcctga
     8101 tcggtatctg gggcgggccc agaaagattt atgcggctat caagttcttc ctttatacat
     8161 tgttcggatc gatcttcatg ctggtcggaa tcctgatcct ctatttctcc tctgtcgacc
     8221 cgagcacggg agcacacata tacacgctcg acctgaccat cctggcccag atggcgcaga
     8281 atggcaacgg ccttttcctg cgcgagggac tccagcttct ggtctggatg ttcttcttca
     8341 tagcatttgc aatcaaggtt cctgtctggc ccttccatac atggttgccc gatgctcatg
     8401 ttgaggcgcc tactgccgtc tcggtaattc tggccggtgt gctcttgaag atgggtatct
     8461 atggacttct gagaatatca tttccgatcc tgccttacgg caccgattat ttctccactt
     8521 tcctggcggt actgggggtg atcggtatta tctacggcgc gctggtttcc atggcgcaga
     8581 aggacctgaa aaaacttgtg gcttactcct ctgtctctca tatgggttac tgtctgctcg
     8641 ggatgagcgt gttctcccat ttcggccagg agttcatcgt gggaatttca ggagcaatgt
     8701 tccagatgat ttctcatggc ctgatcacag gcgccctgtt cttgctggtc ggggttattt
     8761 acgatagggc gcatacccgt gagattgcgg ctttcggcgg actgtggacc aaagtgccgg
     8821 tgtatggctc gttgatgaca tttttctgca tggcctcact cgggcttccc ggcttatccg
     8881 gttttatctc cgaattcctg gttttcgtcg gcggctttgc caagtacaag gtcctgaccg
     8941 gtattgcagt cctgggtgtg gtcctgacgg ccggatattt cctgcggatg atccagaagg
     9001 tatttctggg acagttcaac attaaatggt cggatcttac cgagatcaat ttccgcgaga
     9061 tattcacggt aaccccgctg gcaatactga ccctcctgat cggcatttat ccgaaagtat
     9121 tatcatatat gatcgatgac accctgggtg acctttccaa ctcgattatc aacggtgtca
     9181 atatcgtcaa agggatcttg ggatgaacat cagactcgac ttaatgattc cggagctgtt
     9241 tctcttttgc tgggctattg ttgtcctgac cgcagacctg ttcttcataa aggaaaagaa
     9301 gaatatcctg ctctatatgt cgcaattcgg atttctggcc gccgctgcac tggttatttt
     9361 tactcccacc ggtccgtacg agatggcttt taaatggatg ttcatcaatg atccttttgc
     9421 ctcgtttttc aaaatcgtaa ttctactggc gggctttatg gctgtcagtt catcatacga
     9481 ggctgtccag aaatctattg agcataaggg ggaattcttc gggcttgtca ttatgtctgc
     9541 tgtgggtatg atgttcctgg tatcctcagg ggaactggtt tcgctttatg tcgctctaga
     9601 actttcgaca attccgcttt ttatcctgac cgcattccag aaaagatacc tgaaatcatc
     9661 ggaggccgga ctgaagtacc tgattctcgg cgcattttca tcggcgctgc tgctctacgg
     9721 catcagtttc atgtacggca tgaccggcac taccgttctg atcgcgggtt cgatcaacat
     9781 cctgcagtac tccaacagca tgcactttgg cctggtaatg gcgctggcgc tggttgtggc
     9841 aggcctgggc ttcaagctgg ccctggtacc attccatatg tgggcgcctg atgtttacga
     9901 gggggcgcca acgccgatta catcctatct gtcagttgcc tccaaggcgg ccggcctggg
     9961 cgctctggtt cgtatcatct atggtttctt cacggtcgga accgcgtctg actactgggg
    10021 ccttctgatc gctattcttg cggccatggc catgatcgtc ggaaatgtcg ccgccatact
    10081 gcaatcgaac atcaaacgca tgctggccta ttcctcgatc gcacaggcgg gatatatcct
    10141 ggtcggattt gtagccgcct cacagcttgg cctctccggg atttcaatct atatctttgc
    10201 ttatcttttc gccaatatgg gagcctttgc ggtagtagtg gccgttggac ataaactcaa
    10261 cagcgataaa attgaggact atgcgggcat ggccagacgc tctcccttga ttgccggatc
    10321 tatgaccgtg ttcctgcttt cactggctgg aattccaccc accgccggtt ttatagcaaa
    10381 gtacaaagtt tttatggccg ccattgaaaa cggcacatta ctctggctgg tcatgattgc
    10441 agtggcaacc acggttgtgt caatttttta ttacgcgcgg gtaatcaaag agatgtttat
    10501 cacagagcgc gaggtggctg ccaatcctga aggacgatgg attctatcat caccgatcac
    10561 tatagcaatc ctggtggggt tactcggcac cctgataatc ggaatatttc cccagccgtt
    10621 tctggagctg gccagtacag tcactcaatc atttgtctat taatcttgac atccccggcg
    10681 gaataattta attattgctg ggtattgtta tgaatcgaga cgagaaacct ccaagttgta
    10741 atttcagtat tcaggacggt atacgctgtg cctcacccat atctgccgct tggatcgacg
    10801 taaattccat caaaaacagc ctcgctcgtg caggaattct tgcaatcgat atcaaaaatc
    10861 gatatatttt taaaatgaag ggatcggacc atggattggg tcatggattt tattcaatac
    10921 actggactga atccggggcc gatttaaccg atatatctta gaggatggat ataaagtatg
    10981 agactttcca gaattcttga caaagatctg gttgttccaa gactgaaatc tcgttataag
    11041 ctcgacgcca tcggcgaaat ccttgacaga gtcatacaga aataccctca tctggacaga
    11101 cagacccttg aagctaaaat aattgaaagg gagaacatcg aaaatacatc ctacggccgt
    11161 ggatttgcat ttccgcatgc ccgcactgat gctgttgacg aaatgtacac agcactgggt
    11221 ctggcaccgg agggactcga ggatagaacc aaggaccagc aaaaactgca ggtcatctgc
    11281 ctgatgctga cgccatccac catctcaaag agctaccttc agacactctc ggcctttgcc
    11341 agctttgcca gaaccccagg caataccgag agattgttga gttccaggac cgttgatgag
    11401 atcatagatg tcatcgagga atccggggtc gaggtaaaaa gagagctgtc cgtgggcgac
    11461 gtcatgaagc gcgatgtgat cactgtcaaa ccccaggatt cattgaaaac cgttgccaat
    11521 gtgatgttca agcaccgaat ttcaggagta attgttgtcg atgatgaaaa tcatgtggtc
    11581 ggaatgatct ctaaccgtga tatcatcagg gccgcccttc cggattacca gtcgcttata
    11641 tcaaacctgg ccatgtccta tgattcggag tcgttcgaga atatcctgcg caccgaggaa
    11701 aatgttcgcg tcggcgatct gatggataag aatgtcgaga ccgtaacaga ggatacctct
    11761 gtggtagaga ctgcggcctt gatgctcttc aaggacttga ggcgggcgcc tataatc
//
LOCUS       DSEB01000178            1989 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1010219, whole genome shotgun sequence.
ACCESSION   DSEB01000178 DSEB01000000
VERSION     DSEB01000178.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1010219"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            65..>1989
                     /locus_tag="ENO07_03265"
     CDS             65..>1989
                     /locus_tag="ENO07_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01016.1"
                     /translation="MDVKLPRLREDLIISEQVQAGQTVYIVKDPLSNRFFRLREPEYF
                     LASSLDGEKSPDEIRSEFNTKFGTNITPEQLNAFIDGLERKNFLETSRAEYAVTHEVG
                     DQDARSFISKILFLKLKAFDPSSFLEWLYRKFRFTLSVPFVVFSFIVIIAGILQIAAI
                     FPEMPHTVYQIFGISSIPIIIIAIFIIVALHELAHALVCRHYGGRVSEMGFLLLYFQV
                     CFYCNLSDSYLFEKKRHRIYTILAGLYMQAFVGAASILLWRILKTGTTISDILFLTAS
                     VAFITLLFNLNPLLKLDGYYLLTDIVEIPNLRQKAFGYLKSLLKSIFVGPDYFRRRFT
                     PRERRVFLTYSLLSILYSLILFVWLGSLVYRLLVGAWGGTGFLLFLALLLIIFNQPIS
                     NLFKWIGRIFSRQGVSMPKSLRFYIWLTVLAALVLLAIFYPVDLKINAPVSIEPLEKF
                     IVRGGNNNQLETKWFQGGISQKPINRVYQFSISDFAVLDIEPTMTVGERIDSGDILLQ
                     ISSNHFQSQLEQTSAEIQRARAEYELLLSDPKAAELARASASLEEEKLRQINVEQEYQ
                     RARKLYEKGLISEEEWETAKTTRYVQNKKVDISQSEVDLLKEGPKAEELFSQEAEIRR
                     LESKAEYLRDQIAACTI"
BASE COUNT          504 a          511 c          471 g          503 t
ORIGIN      
        1 aaaaagtgct atatattcag cctataacag gcgagttatc gataaaatca gcaaagctta
       61 aaacatggat gtcaaactgc ccaggctgcg cgaagacctg atcataagcg agcaggtcca
      121 ggccggacaa actgtctata ttgtcaagga cccgctctcc aatcgcttct tcaggctcag
      181 agagccggag tattttctgg ccagctcact ggatggtgaa aagtctcccg acgaaatccg
      241 ctccgagttc aatacaaagt tcggtacgaa catcacgccc gagcagctca atgcctttat
      301 cgacggcctg gaaaggaaaa attttctcga gacttccagg gccgaatacg ccgtcacgca
      361 tgaggtcggg gatcaagacg cacgatcatt cataagcaaa atactgttcc tcaaattgaa
      421 ggctttcgac ccctcgagct tcctggaatg gctgtaccgt aagttccgct tcaccctcag
      481 cgttcctttt gttgtgttct cctttattgt aattatcgca ggaatacttc agatcgccgc
      541 gatatttccc gagatgccgc atacggtcta tcagatattc ggaatatcct ccataccgat
      601 tataataata gcaattttca tcatagttgc cctgcatgaa ctggcccatg ccctggtctg
      661 ccgtcattac ggcggacggg tgagcgagat gggtttcctg ctgctttatt ttcaggtctg
      721 cttttactgt aatctctcgg attcatatct cttcgagaag aaaagacatc gaatctacac
      781 cattctggcg ggattataca tgcaggcctt cgtcggggct gcctcaatat tactgtggag
      841 aattttaaaa accggaacca caatctccga tattctattt ctcacggcct cggtagcatt
      901 tatcaccctc ttattcaacc tgaatccatt attgaagctc gacggctact atctcttaac
      961 agatatcgta gaaatcccca atctgcgcca gaaggctttc ggctacctca agagcctgtt
     1021 gaaatcgatc tttgtcgggc cggactattt ccgccgcagg tttaccccgc gtgagcgcag
     1081 agtattcctt acatactcgc tcctgtccat cctgtattcg ctgatactgt ttgtctggct
     1141 ggggagcttg gtctaccggc ttctggtggg ggcctggggc gggacaggtt ttctattgtt
     1201 tttagctttg cttcttatta ttttcaatca accgatctca aacttattta aatggattgg
     1261 gaggattttc tccagacagg gagtgtctat gccgaagtct cttaggttct atatctggtt
     1321 gacagttctg gccgcactgg ttctgctggc catattctat cctgtcgacc tgaagatcaa
     1381 cgcccctgtt tcaatcgaac ccctggagaa attcatcgtg cgcggaggca ataacaacca
     1441 gcttgaaacc aaatggttcc agggcggcat cagccagaaa ccgataaatc gtgtctacca
     1501 gttcagcatc tcagattttg ccgtactcga tatcgaacca accatgacgg taggcgagag
     1561 aattgacagc ggagatatcc tgctgcagat ctcctccaac catttccaga gccagctgga
     1621 gcaaaccagc gcggagattc aacgcgcccg cgccgagtac gagctgcttc tttccgaccc
     1681 caaggcggct gagctggcgc gcgccagtgc atccctggaa gaagagaagc tgaggcagat
     1741 caatgtggag caggaatatc aacgggccag gaagttatat gaaaagggcc ttatttcaga
     1801 agaagagtgg gagaccgcca aaaccacccg ctacgtccag aataagaagg tcgatatctc
     1861 acagtccgaa gtggatttat tgaaagaagg gcccaaagcc gaggaactgt tcagccagga
     1921 ggccgagatc aggcggctgg agtccaaagc tgaatatctt cgcgatcaga tagccgcctg
     1981 caccataag
//
LOCUS       DSEB01000179            1989 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1010216, whole genome shotgun sequence.
ACCESSION   DSEB01000179 DSEB01000000
VERSION     DSEB01000179.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1010216"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="ENO07_03270"
     CDS             complement(<1..1091)
                     /locus_tag="ENO07_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylan 1,4-beta-xylosidase"
                     /protein_id="HDS01017.1"
                     /translation="MTKKVEVPVSETPFKNNATYCVGTGRMGLALQKEYLDHLQIVQE
                     AIHFRHIRGHGLFCDDVGIYRELEMDGETHPFYNFTYLDRIIDAYLELNIRPFLELGF
                     MPDKLKTGEQAIFYWKGNVTPPVSYEKWADLIKALLQHCIERYGREEVVTWPIEVWNE
                     PNIPFWAGTMEEYFKLYDVSAEAVKQIDPRIQVGGPAICGVQTGKWLRAFFEHCIEAH
                     SPLDFITRHCYTANVPTRRGEYAYHTVNAPTIMIEELKETHRYMADYPQIANLPLHIT
                     EFNSSYVPNCPIHDTDFHAPYIARILSEAGEYADSYSYWTFSDVFEEADVPKSVFHGG
                     FGLVALNGIKKPTFYAFEFFSRAGKELLYR"
     gene            complement(1284..1937)
                     /locus_tag="ENO07_03275"
     CDS             complement(1284..1937)
                     /locus_tag="ENO07_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01018.1"
                     /translation="MPDFDPSYSRSSYQIIRSHFYEEEWWKPYDLIEFLIKRIPERWR
                     EQLVMILNKFMQDENSAYRIVDDEVVEITDEYEIEAIESALDHSIRASRSHLSRALDL
                     LSDRKHPDYRNSIKESISAVEAACQTLAGMPNATLADCIKVIKNRRTIHPAFEQALLK
                     LYGYTSDEGGIRHALTEDSIGPLFSDAKFMLVTSSAFVNFICTKASELGIDIKVEVE"
BASE COUNT          511 a          505 c          478 g          495 t
ORIGIN      
        1 cgatagagca gttccttccc ggcgcgggag aagaactcaa aggcatagaa ggtcggcttt
       61 ttgatcccgt tgagggctac caggccaaag ccaccgtgga agacagactt gggcacatct
      121 gcctcctcaa agacatcgct gaatgtccaa taggaatagg aatccgcata ctccccggcc
      181 tcgctgagga tgcgcgcaat gtacggcgcg tggaaatctg tatcatggat cgggcaattg
      241 ggtacgtaag aactgttaaa ctcggtgata tgcagcggca gattcgcaat ctgaggataa
      301 tccgccatat aacggtgggt ttctttgagt tcctcaatca taatggtagg agcgttgacc
      361 gtatggtaag cgtactctcc gcgccgggtc ggcacattcg ccgtatagca gtgtcgggtg
      421 atgaaatcca gcggcgaatg ggcctcgatg caatgctcga agaaggcccg cagccatttt
      481 cccgtctgca caccgcagat ggccggtccc ccgacctgaa tgcgcggatc gatttgctta
      541 acggcctcag ccgaaacatc atacagcttg aagtattctt ccatcgtccc ggcccagaag
      601 gggatattcg gctcattcca cacctcgatc ggccaggtga ccacttcctc gcggccatac
      661 cgctcgatac agtgctgcaa caaggctttg atgagatcgg cccatttttc gtaggagaca
      721 ggaggtgtca cattgccttt ccagtagaag atcgcctgtt cgcccgtctt cagcttgtcc
      781 ggcataaatc ccagttccag gaatggacgg atgttgagtt cgagataggc gtcgataatc
      841 cgatccagat aggtgaaatt gtaaaacgga tgcgtctccc catccatctc aagttctcga
      901 taaatgccga catcatcgca aaacaggcca tgccctcgga tatggcggaa atgaatggcc
      961 tcctgcacga tctgtaaatg gtcaaggtat tccttttgca gtgccagccc catcctgccg
     1021 gttccgacgc aataggttgc attgtttttg aaaggcgttt ctgaaaccgg gacttctacc
     1081 ttttttgtca tatcgaacct ccttatctgt aaacatgcaa gcgctcccac gccctccagt
     1141 cgggcaaaag gcggtcgagc tgataacgag tccgtccctg tgctggacga gatgcaggta
     1201 gccatctacc aggcggtcgt ggactgaaat cataaccgaa cagcaatgct ccgcaccgga
     1261 aatcggcgcg gggcattgta tgtttattcc acttcgacct ttatgtcaat tccaagttcc
     1321 gaggccttgg tgcaaatgaa attcacaaac gccgaagatg tcaccagcat gaacttggca
     1381 tcggaaaata agggaccgat gctatcttca gtcaaagcgt gccgtatacc accttcatcg
     1441 cttgtatatc cataaagctt caaaagcgct tgctcaaatg cgggatggat agttctccta
     1501 ttcttgatta ccttgatgca atccgcaaga gttgcgttgg gcataccagc aagagtttga
     1561 caggcagctt ccacagcaga aatcgactcc ttgatggaat ttctataatc gggatgcttt
     1621 cgatcagaca acaaatcgag cgctctagag agatgagaac gtgacgctct aatgctgtgg
     1681 tcaagtgctg attcaattgc ttcaatttca tattcatctg tgatttcaac aacttcgtca
     1741 tcaacaatcc gataagcaga attttcatcc tgcataaatt tgttcaaaat catcacgagt
     1801 tgctctctcc atcgttcggg tatccgcttt ataagaaatt caatcagatc gtatggtttc
     1861 caccattctt cttcatagaa atgagaacga atgatttggt atgatgaccg tgagtaagac
     1921 ggatcaaaat ctggcatcgt atcaatcggc cacttgaaat agtttagcca aatcaatgag
     1981 acaacgtcc
//
LOCUS       DSEB01000180            1553 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1017737, whole genome shotgun sequence.
ACCESSION   DSEB01000180 DSEB01000000
VERSION     DSEB01000180.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1017737"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(33..1286)
                     /locus_tag="ENO07_03280"
     CDS             complement(33..1286)
                     /locus_tag="ENO07_03280"
                     /inference="COORDINATES: protein motif:HMM:PF13439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01019.1"
                     /translation="MPQKAKRVLIVSYYFPPMGLGGVQRAAKTAKYLAQEGWHVHVLA
                     CNPDNYPIRDESLLNDLPEEVEITYLDDPVARHSRPSAEHDYILRGRSHLLRRIVQIP
                     DSKKFWADRATKTAEKIIHDHSIAYLLTTSPPPSTHTVGIDLKRDFCVSWLADFRDPW
                     FADSEKPITVLHTALQQNLEKNIIQYADRIVGVTSAHVRDLQKRYARFKDKIHHIPNG
                     FDGDDFKNVEESFPEKLILAHGGTLCSPHTAGAFFEALLRLESEMLNQTEFWQIGAVD
                     QEIHDMLLTRFADKIKIEFTGYMNHRDTLRQLAKSSAIVVFGGVDKKSLNIIPAKLYE
                     GLAFKKPLLAVVEKTSAVHEVISRLQGVHHLDPDDSEGMKNLLKKIVDDHKAGTLFNQ
                     SRLSQLRKYERKYQARQMAELMENI"
     gene            complement(1270..>1553)
                     /locus_tag="ENO07_03285"
     CDS             complement(1270..>1553)
                     /locus_tag="ENO07_03285"
                     /inference="COORDINATES: protein motif:HMM:PF00534.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDS01020.1"
                     /translation="TSVSLLEAMASGLFPIVSDIGGNREWIRHGVNGLMFEVGNGLHL
                     AEMIMQAAGDFELRKKAAHRNFEIIQEKAIWEDNMAVVEKEFESLAAKS"
BASE COUNT          360 a          394 c          364 g          435 t
ORIGIN      
        1 attgtagaga ggtataatca cgctgatcag catcagatat tctccataag ttcggccatt
       61 tgccgagcct gatatttgcg ctcatatttt cgaagctgcg agagccgcga ttgattaaaa
      121 agagttccgg ctttatgatc atcgacaatt tttttcaata agttcttcat gccttcactg
      181 tcatccggat cgagatgatg gacgccctgc agccggctta tgacctcatg aacggcgctg
      241 gttttttcca ccaccgcgag caatggcttt ttgaaagcaa gcccctcata taatttggcc
      301 gggataatat tgagactttt tttgtccacc ccgccgaaga ctactatggc cgacgatttg
      361 gcgagttgcc ttaatgtgtc cctgtggttc atgtatcccg tgaattcaat cttgatttta
      421 tcggcaaagc gggtgaggag catatcgtga atttcctgat cgaccgcacc tatctgccag
      481 aactctgttt ggttcaacat ctcactttcc agtcgcagca gggcctcgaa gaatgccccg
      541 gcggtatgag gactgcagag cgttccgcca tgcgcaagga tcagcttctc aggaaatgat
      601 tcctcgacgt ttttaaaatc atctccgtcg aacccgttag gtatgtgatg aatcttgtct
      661 ttaaaccggg catatctttt ttgcagatca cggacatggg cagaggttac gccaacaatt
      721 cggtcggcat attgtattat gtttttctca agattttgct gcagcgccgt atgcagtacg
      781 gttattggtt tttcggaatc ggcgaaccat ggatccctga aatcagccag ccaggagaca
      841 cagaaatcac gtttaagatc gattccgaca gtatgggtcg agggcggcgg cgaagttgtc
      901 agaagatacg cgatcgaatg atcatgaatt attttctcgg cagtttttgt tgccctgtcg
      961 gcccagaact tcttcgagtc cggaatctgt acaattctcc tcagcagatg tgatcggccc
     1021 ctcaatatat agtcatgctc cgcgcttggt ctggagtgcc tggctacagg atcatccaga
     1081 taagttatct caacttcctc aggcagatca ttaagaagcg actcgtcccg gatcggatag
     1141 ttatcgggat tacatgccag gacatggaca tgccatccct cctgcgccag gtatttggcg
     1201 gtcttggcgg cacgctgcac ccctcccaga cccataggcg ggaagtagta tgagacgatc
     1261 aggactcttt tagctttttg cggcaagcga ttcgaactcc ttttccacta cagccatgtt
     1321 gtcttcccat atggcctttt cctggatgat ctcaaaattt ctgtgcgccg cttttttgcg
     1381 taactcgaaa tctcctgcgg cctgcattat catttctgcc agatggagac cattaccaac
     1441 ttcaaacatc aacccgttga ctccgtgccg gatccattcc ctgtttccac cgatatcgga
     1501 gactatcggg aaaagtcctg aagccatcgc ttccagaagc gacacagagg tag
//
LOCUS       DSEB01000181            1553 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1017734, whole genome shotgun sequence.
ACCESSION   DSEB01000181 DSEB01000000
VERSION     DSEB01000181.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1017734"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            325..702
                     /locus_tag="ENO07_03290"
     CDS             325..702
                     /locus_tag="ENO07_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HDS01021.1"
                     /translation="MEGIMKTVTVLLTGVFLLLLAGNLPAVQTPSSLLRLSDLANQSG
                     TLSVPANDADDYLSESIAPMGTRGGGTYDFNDKISSKIEIDGSKYSNDAGGIPVPTPV
                     PEPTTLILIGLGLAGAGILRRKN"
     gene            complement(830..>1553)
                     /locus_tag="ENO07_03295"
     CDS             complement(830..>1553)
                     /locus_tag="ENO07_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016420455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HDS01022.1"
                     /translation="DAMIRIDMSEYMEKFSVSRLIGAPPGYVGYDEGGQLTESVRRKP
                     YSVVLLDEIEKAHPEVFNILLQVLEDGRLTDNQGRTVSFKNVIMIMTSNLGASEIMEM
                     SKEIESVGFDVVYSRMKRKAMELLESNLRPEFLNRIDEILVFEPLSKAHLKQIVRIQF
                     EIIRKRLHDQKIDAELTEKAVDYLGEAGYDPSYGARPLRRLLHREVVNHISKEIIKGN
                     LKSGDRVVIDASGDGLIFRAGN"
BASE COUNT          398 a          390 c          342 g          423 t
ORIGIN      
        1 ggaattcaac aaaaatcctg gaaatgccgg ggaaattatc aagtcatatc gggaaatacc
       61 tgaaattgcg ggtatttaag ctgattatat cctgaatgcg gaaactgtcc agttttctga
      121 cagaattcat gatgggctta agcttataaa tggcaaagag taaggtgcgc cgcatgaact
      181 ctgctcgggc gggactgtcg gtctgcctga cacaactttc ggtatctctg caagaagtct
      241 tttctaatcc tttgcggaac aaggatttat gttttggcac acaatttgct tatttttaag
      301 ttaggaagat actgaatctt aattatggag ggcattatga aaaccgtaac agtactactt
      361 acaggagtat tcctgctcct gctcgccgga aatctgccgg ccgttcaaac tccttcttcg
      421 ctattgcgac tgtctgatct ggcaaatcag agcggtaccc tgagcgttcc tgcaaatgat
      481 gcagacgatt atctctcaga gtctatcgca ccgatgggta cgcgtggcgg gggcacatac
      541 gatttcaatg acaaaatatc gagcaagatc gagattgatg gcagcaagta ttccaatgat
      601 gcaggtggga ttcccgttcc gactccggtg ccggagccaa ccactctgat tctgattggc
      661 ctgggtctcg ccggagcagg tatcctgcga agaaagaact aagtattagt tttgactaat
      721 aaaaaaggca gctttttaaa gctgcctttt ttattttcct gtatcagcag tcttcccgaa
      781 aaagggaagc gattgcgaat cctgcttcgc tcagaacaac tgctctatgt taattccctg
      841 ctctgaaaat caaaccgtca ccggaggcat caatcaccac tctatcgccg gatttcaggt
      901 tgcccttgat aatctctttg gagatatggt tgacaacctc tctgtgaaga agccgccgca
      961 acggtctggc tccgtaagag ggatcgtacc cggcttcgcc cagataatcg accgcttttt
     1021 cggtcagctc cgcatcgatc ttctggtcat gcagacgttt ccggattatc tcgaactgga
     1081 ttctgactat ctgtttcaga tgagctttgc ttaatggctc gaatacaaga atctcgtcga
     1141 tcctgttcaa aaattccggt cgaagattgc tttccaaaag ctccatggct tttctcttca
     1201 tgcgagaata aaccacatca aaaccgaccg actcgatctc tttggacatc tccataatct
     1261 ctgatgcccc caggttcgag gtcattatca ttatcacatt cttgaacgaa acagtccgtc
     1321 cctggttatc ggtcaggcgg ccatcctcca gtacctgcag gagaatattg aacacctccg
     1381 gatgcgcttt ttcgatctca tctaaaagca caaccgaata cggcttccgt ctcacagatt
     1441 cggtcagctg cccgccttca tcatacccga catacccggg aggcgccccg atcagccttg
     1501 agaccgaaaa tttctccatg tactcggaca tatctatacg aatcatggca tcc
//
LOCUS       DSEB01000182            2518 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1006081, whole genome shotgun sequence.
ACCESSION   DSEB01000182 DSEB01000000
VERSION     DSEB01000182.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1006081"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..362)
                     /locus_tag="ENO07_03300"
     CDS             complement(<1..362)
                     /locus_tag="ENO07_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01023.1"
                     /translation="MRNTDKVLKLEEEISKNIDELTSANRRLRKKIFDLYTIFEISRH
                     LNSVLDTDSLLDGILLTIIGQMGVSGCAIFTVDKDGKALTIAKSRGLTLSKVKTSHIP
                     LDSRLFQATDEKRSTAYN"
     gene            complement(414..1691)
                     /locus_tag="ENO07_03305"
     CDS             complement(414..1691)
                     /locus_tag="ENO07_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDS01024.1"
                     /translation="MTYKEALDFLYSLELFGVKLGLNNITRFLNRLGNPQKSFKGIHV
                     AGTNGKGSVASMFESVLHEAGYKVGKFTSPHLYNFRERFHIDKQKVDETFVAEFIEEH
                     QEYIRDSRTTFFETCTALAFELFRRNKVEYAVVEVGLGGRLDATSLIEPELTVITKIA
                     KDHTKTLGDSIAKIAFEKCGIIKQGVPLVTSVADPEALQVIREVSESKKAPLYCLLPA
                     SMVEVMSLTVEKMRFNFSENSVPPKNYVGNLVGTHQAENYGLALLGLKVLSQRGLRIP
                     EDAYQSGIANVFWPARLQFVPGKPNLLLDCAHNPDGVRALVKNVEAIYPGRKFVLILG
                     MLRRPDFPEIFEEIKKISNKVILTIPDHERAPEPEVLAREAIESRLNFKLIPNVIDAY
                     KFARELVSDNEILLVTGSHFTLGEIMKFENIPT"
     gene            complement(1669..2496)
                     /locus_tag="ENO07_03310"
     CDS             complement(1669..2496)
                     /locus_tag="ENO07_03310"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDS01025.1"
                     /translation="MISQKKKDIPEGLWTKCPRCSDIIYVRQLEENLSTCPNCDYHFR
                     IKSSDYLKLILDEGELEERDQGLISGDPLQFKDSKKYPDRIKSARKNTGLNDAVVCGI
                     GKIDGRTVSIAVMDFAFIGGSMGSVVGEKIARTIERAIQMQIPLLIVSCTGGARMQEG
                     ILSLMQMAKTSALLAQLDDLRLPYISVLTNPTTAGVMASYASLGDVIIAEPGALLGFA
                     GQRVIKQTIRQDLPEGFQSSEFFQEHGFLDKIVHRRDLKKTISLFLDYFSNDLQGSA"
BASE COUNT          606 a          660 c          577 g          675 t
ORIGIN      
        1 atattatatg ctgtactgcg cttttcatcg gttgcctgga aaagcctgct gtcaagtggt
       61 atatggcttg tcttgacctt actgagcgtg agccccctgc ttttggcaat agtcaaagct
      121 ttgccgtctt tatcaacagt aaatatggca catcccgaca cccccatctg cccgataatt
      181 gtcagtaaga ttccatccag gagactgtcg gtgtcgagaa ccgaattcag atgccgggat
      241 atctcgaaga tcgtatataa atcgaagatc ttcttgcgca gccttctgtt ggccgacgta
      301 agctcatcga tatttttcga gatttcctct tcgagcttca agaccttatc ggtatttctc
      361 attttttcgt aatcttcatc catattttca accatctttg accattcatt tatttaggtc
      421 gggatatttt cgaatttcat gatctcgccc agggtaaaat gtgaccctgt gacaagcagg
      481 atctcattgt cactcaccag ttcccgggca aatttgtatg catctataac atttggtatc
      541 aatttgaaat tcagtcttga ctctatcgcc tcccgcgcca gcacttcagg ctccggggcg
      601 cgctcatggt ccgggattgt caggataacc ttattggaaa tcttcttgat ttcctcgaaa
      661 atctctggaa aatccggacg gcgcagcata cctaatataa gtacgaactt ccggccggga
      721 tagattgcct ctacattctt gaccagcgca cgcactccat cgggattatg agcacagtct
      781 aatagcaggt tcggctttcc cggcacaaac tgcaggcggg caggccagaa aacgtttgcg
      841 ataccgctct gatacgcatc ttcaggaatc cggagtcctc gttgcgaaag gactttcaaa
      901 cccagcagcg ccagtccgta attttcggcc tgatgtgtcc ccaccagatt gccgacataa
      961 tttttcgggg gaactgagtt ttcgctgaaa ttgaacctca tcttttcaac agtcaggctc
     1021 atgacctcga ccatgctcgc gggcagaagg cagtacagcg gggctttttt actttcagat
     1081 acttcccgga tcacctgcaa tgcctcggga tcagccaccg aggttacaag cggaacaccc
     1141 tgcttgatta taccgcattt ttcgaaggct attttggcaa tcgaatctcc cagtgtttta
     1201 gtatgatctt tggcgatttt ggttatcacg gtcagttccg gttctatcag cgaggtcgcg
     1261 tccagacgcc cgcccaaacc gacctccacc acggcgtact cgactttgtt gcggcggaag
     1321 agctcgaagg ccagggcggt gcaggtttcg aaaaaggtcg tgcgggaatc cctgatatat
     1381 tcctggtgct cttcaataaa ctcggccaca aaagtctcgt caactttttg tttatctatg
     1441 tgaaacctct ccctgaagtt atagagatga ggagaggtga acttccctac cttatagccc
     1501 gcttcgtgca ggaccgattc aaacatggag gccaccgagc ccttgccgtt agtaccggcg
     1561 acatgaatcc ccttgaatga tttctgggga tttccgagcc ggttcaggaa tctggtgatg
     1621 ttgttcagac cgagtttgac accaaacagc tcaaggctat atagaaaatc aagcgcttcc
     1681 ttgtaagtca ttggagaaat aatccaaaaa taatgagatt gttttcttga gatcacggcg
     1741 atgcactatt ttatccagga agccatgctc ctggaaaaac tcggagctct ggaaaccttc
     1801 gggaagatcc tggcggattg tctgtttgat aacgcgctga ccggcaaaac ccaggagcgc
     1861 tcccggctcg gcaataatca catcgcccag agaagcataa ctcgccatca caccggcggt
     1921 agtcggattg gtgagaacag atatataagg caggcgcaaa tcatcaagct gagccagaag
     1981 ggccgaggtc ttggccatct gcatcaagga caggattccc tcctgcatgc gggcgccgcc
     2041 cgtgcaggat actatcagga gcggaatctg catctgtatg gccctctcga tagtgcgggc
     2101 aatcttctcc cccaccacag aacccatact gccgccgatg aaagcgaagt ccataaccgc
     2161 aatcgagaca gttcggccgt caatctttcc aatcccgcaa acaaccgcat cattaaggcc
     2221 ggtgtttttt cttgcgctct tgatccggtc cggatatttc ttagaatctt tgaactgcag
     2281 aggatcccct gaaatgaggc cctgatccct ttcttccagt tccccctcat cgagtatgag
     2341 cttcagataa tcagaactct ttatgcgaaa atggtagtcg caattcggac aggttgacag
     2401 gttttcctcg agctgtcgaa cataaattat gtcgctgcaa cggggacatt tggtccacag
     2461 tccctcagga atatccttct tcttttgcga tattatgcct tcccgtgcct tcttaaac
//
LOCUS       DSEB01000183            4338 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001820, whole genome shotgun sequence.
ACCESSION   DSEB01000183 DSEB01000000
VERSION     DSEB01000183.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001820"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..319)
                     /locus_tag="ENO07_03315"
     CDS             complement(<1..319)
                     /locus_tag="ENO07_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01026.1"
                     /translation="MKQIILIAITIFAIASISAVADDPKAGEEINWQVLSNGGTEGSS
                     TNYGLKGTVSQTAVDQGSSTNYKLRHGFWQIFATSGGPCQGECGDANNDANVNVSDAV
                     WIIN"
     gene            complement(316..2082)
                     /locus_tag="ENO07_03320"
     CDS             complement(316..2082)
                     /locus_tag="ENO07_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01027.1"
                     /translation="MKKLVLCTTLILFLVAVAAYAMNVPNATNYQGRLTDDNGDPVPD
                     GTYNISLNIYHMESGGSPVYTETESVQVTNGLFNCILYIPDYALTDTSFCYLGITVNG
                     EEILPRTMILSSAFAMHAQHGGGWKFNQGGYLSNTIHTDETVEYMKVGINNPNPEEEL
                     TIGSQNLGTFFGNCISISSPDSNSGFLIGKDMSNRVAIFRSVDGRFLINTVQGGEYYY
                     PVRINQDKVGIDTWPDDPSEPLVVGKDLGSFSGDRIVIGDDAAGAQTGLVFGESGSDR
                     AWMLWDVDEDMVTLGSKHSGTNYSDALTIQNGCVGLGIEPQTYTVLRIDRTYNSSLSR
                     YASIAQAVQGGTGSAFGMWSKAQRTSSSSTSNYTYGVYGIGQNDFSVGSAVGVYGYAT
                     GGDFTRGVMADVPGYHEDLDNAGYFLGDVAIQGEIVYLFGAYRVDHPLDPENKYLYHS
                     NVGSPDMMNVYTGNVVTDASGQATVNLPEYFEHLNSDFRYQLTVIGTFAQAIVKEEIS
                     NNSFVIATSEPNVKVSWTVTGIRNDPYANARRIVPEREKHVLERGTYLFPQGFGLGEE
                     RSVDYNSRQAAREAAEAGGEEQ"
     gene            complement(2086..3729)
                     /locus_tag="ENO07_03325"
     CDS             complement(2086..3729)
                     /locus_tag="ENO07_03325"
                     /inference="COORDINATES: protein motif:HMM:PF13884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HDS01028.1"
                     /translation="MNHKFIIGILGIIMLISTTFLSADSSRTINYQGYLTDSSGEPVN
                     GTIGVSFYIYESLLTQMWMWSESHTVECENGLFSVVLGQTTPFQDSVFNGEDRYLGIR
                     IGDDSEISPRTLITAVPHAFISQQMSGDIETESGKLVMKDIDGDSVIVMESNFDGTKS
                     IVGSIKMFNPQPEPPAKMFEVNADATSGASLNLYNEIGQVMGFEPTPFNDGYLMKFID
                     PGDDGKLLEMFGNHQSNEAGLYLIDPGDDGHIVSLTSSPTSGGSIKMFNPQPEPPALI
                     MEIASDLSAKGGSASISVYDDTQLLESHLEPGILNLVSDAGGVPGPSINLTAASTYAR
                     VGIGNDSPNEPISIGANLGSYYGEFVQIGNTDAGRYSGFMCGEDENNRGTILWYNNDN
                     QLRLGSRSAGSDYATTLVIKEDQVAINTTTPGSQELYVVGDIYATGTITELSDVSAKT
                     NIETIGNALELVENLRGVRYNLHSRLAADMKTSADRQVGMIAQEVESILPEVVSSPAD
                     GYKSIDYSRLTAVLVEAVKELKAENEILKQRIEALENAR"
BASE COUNT         1157 a         1117 c          918 g         1146 t
ORIGIN      
        1 agttaatgat ccagactgca tcggagacat tgacattggc atcattgtta gcatcaccgc
       61 attcaccctg gcaggggccg ccggatgtcg cgaagatctg ccagaagcca tgtctgagct
      121 tataattggt cgatgaacct tgatcgactg cagtctggct gacagtgccc ttgagaccgt
      181 agttggtcga tgatccctca gtaccgccgt ttgagagcac ctgccagttg atctcctcac
      241 cggctttggg atcgtcggca acagcactga ttgatgcaat cgcaaagatt gttatcgcaa
      301 ttaatataat ctgtttcatt gctcctcacc ccctgcctcg gctgcctcgc gggctgcctg
      361 tcttgaattg taatcaacag atctctcttc tcctaagcca aaaccttgcg gaaaaagata
      421 tgtcccgcgt tcaagaacat gtttctccct ttccggcaca attctgcgcg catttgcata
      481 tggatcgttg cgaataccgg tcactgtcca ggagactttg acatttggtt cacttgtcgc
      541 aattacaaaa ctattattgg agatttcctc ttttactata gcctgcgcga atgtcccgat
      601 tacggttagc tgataacgga aatctgaatt aagatgctcg aaatactccg gaagatttac
      661 cgtcgcctga ccgcttgcat cagtgaccac attgccggta taaacattca tcatatccgg
      721 tgagccgaca ttgctgtgat aaagatattt gttttccggg tcaagaggat gatcgactct
      781 ataagctcca aacaggtaga ctatttcccc ttgaatagca acatccccaa gaaaatatcc
      841 ggcattgtcc aggtcttcat gataaccggg aacatccgcc atcaccccgc gtgtaaagtc
      901 tcctccggtg gcataaccat aaaccccgac agctgaaccg acactaaaat cattttgacc
      961 gataccgtag acgccgtaag tgtaattgct tgttgacgaa gaggaagtcc tttgggcctt
     1021 gctccacatt ccgaaagcac tcccggttcc cccctgcaca gcttgtgcta ttgaagcgta
     1081 acgagaaagt gagctattat atgtcctgtc aatgcgaagt acagtgtaag tctgaggctc
     1141 tattcccaat ccgacacagc cgttctgaat tgtgagtgca tcgctgtaat tcgtacctga
     1201 atgttttgat cccaatgtaa ccatatcttc atctacatcc cacagcatcc aggcccggtc
     1261 gctgccggac tcaccaaaaa ctaatccggt ttgggcaccg gcagcatcat cgccgatgac
     1321 aatacgatca ccgctgaatg atcccaaatc tttgccgacg acaagaggct cactgggatc
     1381 atcgggccaa gtatcaatcc cgaccttatc ttgatttatt ctaacagggt agtaatattc
     1441 tcctccctgg acggtgttaa ttaagaatct tccgtctaca gatcgaaata ttgcgactct
     1501 attgctcata tccttcccaa ttagaaagcc gctgttactg tcggggctgc tgatggagat
     1561 acagttgccg aaaaaagtgc caaggttctg gctgccaatc gtcagctcct cctccggatt
     1621 ggggttatta attccaactt tcatatattc aaccgtttca tccgtatgga tggtgttgct
     1681 gagataacca ccttgattga atttccagcc acctccatgc tgggcgtgca ttgcaaaagc
     1741 agatgaaaga atcatcgttc gcggcaatat ttcctcgcca ttgacagtga tacctaaata
     1801 acaaaaactg gtatcggtta aagcataatc cggaatatac agaatacaat taaataggcc
     1861 gtttgtaacc tgcacagact ccgtttcagt ataaacaggg gacccaccgc tctccatgtg
     1921 ataaatattc aatgaaatat tataggttcc atccggaaca ggatctccat tatcatcggt
     1981 cagtcgaccc tgataatttg tagcgttcgg cacattcatg gcatatgctg caactgccac
     2041 caaaaacaaa atcaaagttg tacacaaaac taacttcttc atttcctacc tcgcattctc
     2101 gagcgcttca attctctgct tcagtatttc attttctgcc ttcagttcct tgaccgcttc
     2161 gaccaatacc gcggtcagcc tggaataatc tattgacttg tagccatctg cgggcgaact
     2221 cacaacttcc ggcagaatgc tttcgacttc ctgggcaatc atgcccacct gacgatcagc
     2281 ggaggtcttc atatctgcgg ccagtctgga gtgcaggttg taccttaccc cgcgcagatt
     2341 ttccacaagt tcgagggcgt taccgatggt ttcgatattg gtcttggcgg agacatcgga
     2401 aagctctgta atcgtgccag tggcataaat atcgccgacg acataaagct cctgcgatcc
     2461 gggagtggtg gtattgatcg ccacctgatc ttccttgatc accagtgttg tagcgtaatc
     2521 agaaccggca gatctgcttc caagacgaag ctgattatca ttattgtacc agagtatggt
     2581 accccggtta ttttcgtctt ctccgcacat aaatccggaa taccgcccgg cgtctgtatt
     2641 gccgatctgt acaaactcgc catagtatga tcccaggttt gcgccaattg aaatcggctc
     2701 attgggcgag tcgtttccga taccaacgcg ggcataagtg ctcgcagccg tcagattaat
     2761 cgacggcccg gggacaccac cggcatcgga taccaagttc aaaatccccg gctcaagatg
     2821 cgattcaaga agctgggtat catcataaac agaaatcgag gcgcttcccc ctttggcgct
     2881 tagatcgctg gcgatttcca taatcagagc gggaggttcc ggctgaggat tgaacatctt
     2941 gatactgccg ccagatgtgg gagaggaggt aagcgaaaca atgtgcccat cgtcgccagg
     3001 atcaataaga tacaaaccgg cttcattgct ctgatggttt ccaaacatct caagaagctt
     3061 gccatcatca ccggggtcaa taaacttcat taaatagcca tcattaaatg gcgtcggctc
     3121 aaatcccatg acctgcccga tctcattata aagattcagg cttgccccac ttgtggcatc
     3181 ggcgttaacc tcgaacattt tcgcgggagg ctcaggctga ggattgaaca tctttatgct
     3241 tcctacgatc gactttgtgc catcaaaatt tgattccatt acgattactg aatcgccgtc
     3301 aatatccttc attaccagtt taccgctttc agtttcgata tcaccactca tttgctgtga
     3361 aatgaatgca tggggtaccg ccgtaatcag ggtgcggggc gaaatttcgg aatcatcacc
     3421 gatccgaatt cccaggtatc tatcctcgcc gttaaaaacc gagtcctgga aaggtgtcgt
     3481 ctgtccgaga acaacgctaa ataagccatt ttcacattct acggtatggc tttccgacca
     3541 catccacatt tgcgtcaaaa ggctttcata aatataaaat gacactccaa tagttccatt
     3601 tacgggttca cctgaagaat cggtaagata cccctgatag tttatggttc tggatgaatc
     3661 agcggacaaa aatgtggttg aaatgagcat aataataccc aaaatcccaa tgatgaactt
     3721 atggttcatg gaagtcctcc ttatagatag aattatattt tctcaaacgc attgcgggcc
     3781 ggaaaaaacc agaaaagaat agccggtcgg gaccgcccaa ctatgataca tcttattcac
     3841 atatcaggtc tctgcctcaa aacaaatata caattaatac taattctgtc aatttaaaaa
     3901 ccgccctaaa cacccctata gtgtgacaaa acatgcatcc tcccgcggtc gtcaggagga
     3961 gcattaattc attgtcacca gaaatcagat ggacctgatc agcagtccgg caccccgtca
     4021 ccatttgtat cgcagggatt acttcctccg acaaatacat agtttatgat ccagacagca
     4081 tcggaaacat tcacagtact atcgcagttg gcatcacccg attcaatcgg gtcgggcgga
     4141 ttgccgccaa cgaaaacaaa attgatgaca tagactgcgt cgctgacatt aacggtctca
     4201 tctccgttgg catctccgca aagatagaac atgctaccga taatctggtc ggcaaaccat
     4261 tttttggatt tatcaacaat cgactcgagg ctttcacggc tggcatccag tgtgaccgca
     4321 tacgaagtgt atatatac
//
LOCUS       DSEB01000184            2304 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1007391, whole genome shotgun sequence.
ACCESSION   DSEB01000184 DSEB01000000
VERSION     DSEB01000184.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1007391"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..656)
                     /locus_tag="ENO07_03330"
     CDS             complement(<1..656)
                     /locus_tag="ENO07_03330"
                     /inference="COORDINATES: protein motif:HMM:PF02153.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HDS01029.1"
                     /translation="MDKLAFPGKRIAIIGLGLMGGSLAMSLHGKCDDLFGVDRDPETV
                     KYARKLNIFTQVATSPEEILAQSNVVILATPVGTIVNLIQKLPDIHPGSAIVMDIGST
                     KQEILEAYQALPPRFIPIGGHPMCGKEKSSIAHADPAIFDGAVFALSSLERTSQEVCE
                     FADQLVRAIGSKPVWVGAETHDRWVALISHLPYLLSLSLALATPDDAKTLSGPGYNGM
                     V"
     gene            complement(649..1737)
                     /gene="aroF"
                     /locus_tag="ENO07_03335"
     CDS             complement(649..1737)
                     /gene="aroF"
                     /locus_tag="ENO07_03335"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDS01030.1"
                     /translation="MMIIMLANATQEQIDAVVDKVESTDLAAHLSAGEERTIIGVVGD
                     NVRITQDQLLHMPGIDRIIPISKPFKLASREFHPTSTITTFGNVSIGGDNIVIMAGPC
                     SVESRGQLLETAHAVKEAGGHALRGGAFKPRTSPYSFQGMGIEGLELLAEAREIYDLP
                     IVTEVMSEELVPVVAEYADVLQIGARNMQNYPLLQAVGKSSRPVLLKRGLSATINELL
                     MAAEYILSNGNPDVILCERGIRTYETTTRNTTDINAIPVLQSLTHLPVVLDPSHSTGY
                     WEYVSTIAQAGIAAGADGLLVEVHNHPEEALSDGGQSLRPERFAELVRRVEAISKAVG
                     RSDSPIKSRAVWQSRERHKNGHGILRDG"
     gene            complement(1734..2108)
                     /gene="aroH"
                     /locus_tag="ENO07_03340"
     CDS             complement(1734..2108)
                     /gene="aroH"
                     /locus_tag="ENO07_03340"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDS01031.1"
                     /translation="MQGRGIRGAITIEQDKPEEIISATCELLQAILDANPTLRAENIG
                     SVIFTVTPDIVSEFPAKAARVLGWKSVPLMCMQEIPVPGSLSKCIRVLVSWNTKLKQK
                     DVNHIYLKDAVSLRPDLMEGVS"
BASE COUNT          552 a          581 c          574 g          597 t
ORIGIN      
        1 accataccgt tatatcctgg gccggatagc gtttttgcgt catcgggtgt tgccagcgcc
       61 agcgacaaag acagcaggta gggcaaatgg ctgataaggg cgacccaacg atcatgagtt
      121 tcggcgccaa cccagactgg ttttgagcca atcgcgcgta ccagttggtc tgcaaattca
      181 catacctcct gagaagtacg ctcgagcgaa gacagagcaa agacggcccc atcgaaaatc
      241 gctggatctg cgtgggcaat cgaggatttt tctttaccac acatagggtg cccgccaatt
      301 gggatgaatc ggggcggaag cgcttgatac gcctcgagga tttcctgttt tgttgagcca
      361 atatccatga caattgcact gccgggatgg atatccggca atttttgaat cagattaaca
      421 atcgttccaa caggggttgc caggatgacc acattcgact gagcgagaat ttcttcggga
      481 gaagtggcaa cctgagtaaa aatgttcagc ttccgggcat attttactgt ttcggggtcg
      541 cggtcgacgc cgaacaggtc atcacatttt ccgtggaggc tcatcgctag tgagcctccc
      601 atcaaaccca acccaattat ggcgattctt ttaccaggaa aggcaagctt atccatccct
      661 gaggattccg tgcccgttct tgtgcctttc ccgggactgc caaactgctc gggatttgat
      721 tggcgaatct gaacgtccca cagcttttga gatcgcctct acacgccgga cgagttctgc
      781 gaacctttct ggtctcaagg actggccgcc atccgacaat gcttcttcag ggtggttatg
      841 gacttctacc agcaagccat ctgcaccggc cgcgattcct gcctgggcga tggttgagac
      901 atattcccag tacccggtgc tgtggcttgg gtctaaaact accggtagat gggtgagaga
      961 ttgcagaacc gggatggcat tgatgtcggt ggtgtttcgg gtggtagttt catatgtccg
     1021 gatgccgcgc tcgcacaaga tcacgtccgg attcccgtta gagagaatat actcagcagc
     1081 catcagcagt tcgttgatgg tggcgctcaa gcctctcttg agaagaactg gccgtgaact
     1141 tttccccaca gcctggagca aggggtaatt ctgcatattt ctggccccta tttggaggac
     1201 gtctgcatat tctgcgacga cggggaccaa ttcttccgac atcacctcgg taacaatggg
     1261 gaggtcgtaa atctcacgcg cctctgccag cagttccagc ccttcaattc ccataccctg
     1321 gaatgagtaa ggagaagtcc tcggtttgaa agcgccgccg cgcaaggcat gaccgccagc
     1381 ttccttgacc gcatgggcgg tttccaagag ctggccgcgg ctctcaactg agcaaggccc
     1441 ggccatgatt acgatgttgt ccccgccaat cgagacgttc ccaaaagtgg taattgtgct
     1501 ggtggggtgg aattctcgag aagccaattt aaatggttta gagatcggaa taattcggtc
     1561 gatacctggc atgtggagta gctggtcctg tgtaatccga acattatctc caacaacacc
     1621 aatgatggtg cgttcttcac cagcagataa atgtgctgca aggtctgtgc tttctacttt
     1681 atccacgaca gcatctatct gttcttgtgt cgcattggca agcattatga tcatcatgat
     1741 acaccctcca ttagatcggg acgcagggaa actgcgtctt ttaaataaat atggttaaca
     1801 tccttctgtt tcagtttggt attccaagag accaaaacac gaatacattt tgacaggctc
     1861 cctgggacag ggatttcttg catgcacatt agcggcacag acttccatcc tagaacacgc
     1921 gctgctttgg ctggaaattc agacacaata tcaggcgtga ccgtgaaaat aacagaccct
     1981 atattttcgg ctcttagcgt cgggtttgca tccaagattg cctgaagcag ctcacatgtc
     2041 gctgaaatga tttcttcagg tttatcttgt tcaatcgtaa ttgccccgcg aatacctcgc
     2101 ccctgcatga ttctccctaa taaaaaaagc gcaaggtgat ggccttgcgc tagcgttgaa
     2161 aacgttagtt aaccgccatc tggttgaccc actgtgtgtc tgcggagagt attaaaatta
     2221 ttaattgtcc aatttgaatt attgctcatg tgccttgatt tccctgccat gacataatga
     2281 gataatctat atccaatata tcat
//
LOCUS       DSEB01000185           13845 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000147, whole genome shotgun sequence.
ACCESSION   DSEB01000185 DSEB01000000
VERSION     DSEB01000185.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13845)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13845)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13845
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000147"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(197..1021)
                     /locus_tag="ENO07_03345"
     CDS             complement(197..1021)
                     /locus_tag="ENO07_03345"
                     /inference="COORDINATES: protein motif:HMM:PF00801.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HDS01032.1"
                     /translation="MDAIYDGGSYNFYYAALCAGEPNVQPRRDSLNNHTVPTCYFDAG
                     DTVLVGGYSNLTPYINFLDLAGSRQVPEIDLSVSLTYNAKGTITVDVSATLNTFINLA
                     PDRPSVPAGVTQGNLMTEYTYTSSTTDPEEDQLWYRWSWGDGDTTLWLGPYESGAEAS
                     ASHSWSEAGDYYIKVQAKDENDAESFWSGIKFTHFEGLPYICGDANSDETVNISDAVY
                     IINYVFVGGTTPDPLEAGDANCDATVNVSDAVWIINYVFVGGNAPCDSNGDTDPDC"
     gene            complement(1049..1660)
                     /locus_tag="ENO07_03350"
     CDS             complement(1049..1660)
                     /locus_tag="ENO07_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01033.1"
                     /translation="MHPVPKGIIPGRYALVALVAAFLFFTGVTFAADPALTTRLYDYG
                     PGISSQPAKALDFGDNKIALGESGRALYACQLKVYVVEPVSRYRDYGGHNYEFGFLDF
                     AIDTALNLDYQVPFNKTATWDPAQNGIGTIDGSNIMVIAVIFNHMEGFPQFSYIYQGD
                     TGDPFTAYLADACAAAIPGQTGYNETTGGYTHTVFLEECTWDG"
     gene            1982..2419
                     /locus_tag="ENO07_03355"
     CDS             1982..2419
                     /locus_tag="ENO07_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01034.1"
                     /translation="MRFAAILISAAFMIFAFCSFGSSPLRADDGAESEELFVDLDGDG
                     FNDNTPDEDNNSIPDSAEDDKYSDRNKTANALASTEFKPIEGLGEFDLSSLLSNSELF
                     GQLKFLTRAQSCCRGGFEAGSDFGPGNGIGLGAVSGCAGGVCH"
     gene            2436..3521
                     /locus_tag="ENO07_03360"
     CDS             2436..3521
                     /locus_tag="ENO07_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01035.1"
                     /translation="MRYLLIGIFITVFFVFPAYADFGYEVKLSGSVTNNLISDSSELY
                     DSYSTTSAALKYYPFAQLELNLKSEYNYYSNLVGLGNIMGGAEITWIPTSQSSPLALY
                     FNAGYDNRSYRESFSVYNTNVYNGMGSISYRLARRAQVRAGITYEQTEFDNSEGADKE
                     IYGFMFGANVTFLGSNSLDIESGYSFADFISVDEVSIMSWPQVLEETDGDLKSFYISP
                     RYSRPLGSKLGMNLTYTYLSFVDFDDPVVYSYSTGFLSPWASVWDGNSVALNLKSYHI
                     PKMIITAGGGYWNKRLLRTLELPDRIVTREDDYSRYYIGLSWPMPSRSGAYLEPSIQL
                     DIINNTSTDRLYDYSTFSVVVGLTFRR"
     gene            complement(3570..6266)
                     /locus_tag="ENO07_03365"
     CDS             complement(3570..6266)
                     /locus_tag="ENO07_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01036.1"
                     /translation="MINVDKLAVRGYFINDPKFWDCTQISLEHKHSRDNMRKFAPLFL
                     IFLLFAYVAESAYAQSAPASEPDSMFMPTLIYYDFDNGELPPGWVILDGFQDGYTWQF
                     MDMYSLGGYPPFEDSCLIVNSSTSPNAYMDEAVVTAFYHAPSDINIHLRFSNYFSYDN
                     DTPDEVADVDVRNGAGGQWHNVLRMNNLSHGPETRDIDITPYIGSGDSIQVRFHYYLA
                     RNDRWWMIDNFRLYYDCDDPDQDNICSQFDNCPNDYNPNQIDYDDDGIGYVCDTCVDT
                     DGDGYGNPGFAVNLCETDNCPYLYNPDQEDYDGDGIGDECDQCTDTDGDGFGNPGFPN
                     NTCPTDNCPNHFNPYQYDADGDGRGDQCDYCTDTDGDSFGDPGYPASTCMIDNCPDDY
                     NPYQWDMDTDNIGDSCDACTDIDGDGFGDPGFPANTCVTDNCPTIPNPDQLDDDLDGI
                     GNECDDCNDSDGDGYGDPGYPNNVCATDNCPEIANPDQADADSDGLGDVCDDCTDSDG
                     DGYGDPGYENQSCDIDNCPDTANPDQADYDQDGIGDICDECTDTDNDGYGNPGFAGNI
                     CEEDNCPEIYNPDQLDADGDGLGDLCDECTDTDNDGYGDPGYAANTCQTDNCPDISNP
                     DQADADGDGTGDRCDICPAHPEDDCCNPIGENDPPVIDAPPPIVLEPGGDFVFKAIAS
                     DPDCDGSELEISFSNFPSWCLVSGDSISGTAECDYADTSFIVKVSDGELADSALVLIE
                     IDHSNQPPTIIDTLTLVYVRNGWTFTYYPAIQDPDDSVHTIFYEDYPYWCTIINDTLT
                     GTAPQESSTESIDVHVHDYCNSDSYGFAVTVYFCGDTNADDNVNVSDAVAIINFVFAG
                     GLPPQPLESGDTNCDSQINVSDAIYIINFVFVGGPRPCHDCP"
     gene            complement(6424..7515)
                     /locus_tag="ENO07_03370"
     CDS             complement(6424..7515)
                     /locus_tag="ENO07_03370"
                     /inference="COORDINATES: protein motif:HMM:PF13519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDS01037.1"
                     /translation="MRFIYSEWDDAAIQKLQNLKDLMSIFNYLLLQVNGDAEMALKLM
                     RKLQAMGVLPEDFDLDGFEKNLEKSNIVSWQGDNISLTRKGEKSLRQDAFDSIFEHLR
                     KSGAGGHIIPHGGGSSEEALPEKREYRFGDEFNHIDFQNSLMNTIRRTGSLGLNMAEK
                     DLEVYDTEQLTNCATVMLIDISHSMVLYGEDRITPAKQVALAFSELILTKYPKDSLNI
                     VLFGDFAREVKVKDIPYIGVGPYHTNTKAGLEMARNILLKKKNVNKQIFMITDGKPSM
                     ITRRNGSVYKNPMGLDPVIVNRTLDEAIICRKKRIPITTFMITDDPYLQKFVAKLTEL
                     NRGRAYFASSQNLGDYVFWDFVSRRRKKR"
     gene            complement(7518..8996)
                     /locus_tag="ENO07_03375"
     CDS             complement(7518..8996)
                     /locus_tag="ENO07_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HDS01038.1"
                     /translation="MKFAKTLGELKESGYRSRSTKDELRENLIDLIKSGKKRFPGIIG
                     YDRTVLPQLENAILSRHDILLLGLRGQAKTRILRQLGSLLDEYLPVIKGSQLNEDPFK
                     PISDKHKRLAAEAGDELEIDWIHREQRYNEKLATPDVTVADLIGDLDPIKAVREKLDI
                     SNEEVIHWGIVPRTNRGIFAINELPDLQPRIQVALLNILEENDLQIRGFPVRIPLDMM
                     LAFTANPEDYTNRGNIITPLKDRIASQVNTHYPRDLDEAKQITEQEAWKERGVEIKIP
                     DLIKDIVEEIAFRARESEYVDQTSGVSARLTISAYENLLSNVERRVVMNNDKHAHPRI
                     ADLYAVLPSIMGKIELVYEGEQEGPSMVAVNLVGEAIKQTFLRIFPEPRKERKRTPDG
                     DYEEIIPEDSVYEPVIDFFSAGKQIEISDEMPYSKYKKALSDIVGLREIVLKYCDLGE
                     EDEIFTYMEFVLEGLHQMNRIAKESPDNKFIYTDMIQRILRS"
     gene            complement(9373..10725)
                     /locus_tag="ENO07_03380"
     CDS             complement(9373..10725)
                     /locus_tag="ENO07_03380"
                     /inference="COORDINATES: protein motif:HMM:PF03572.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01039.1"
                     /translation="MKMRKAFGGLILLLVTSMIMPLYGQLQRAEPRDETIDSKTQAEI
                     IDSVTRVINEVYVFPDVAEEMAVHVKGMYQKGEYKDITSLSAFSDRLTKDLRDICHDR
                     HLGVRYTPDITMDDIRTEEDTVTDEERQEHFEELSRDNFGFKEIRILPGNIGYLKFNG
                     FVDAYFGGATAIAAMNFLAHVDALIIDLRENGGGSPSMIQLISSYFFNDPVHLNSFYI
                     RKEDTTKQFWTASHVEGPRMANTPIYILTSGYTFSAAEEFTYNLKNLERATIVGETTG
                     GGAHPVQGEIFPNLKVSLRVPFGRAINPITGTNWEGTGVEPHISCAADHALEVARVDA
                     MEKLVAKAESEEEKGMLRWDLDMLKAQLEPVEVPEELMNKYVGDYGPRKIWMEGDELY
                     YQRDDRPKQKLIALSEDIFMIEGVDYFKMKFEEDESGEVIGILGIYKQGFIDRSPKDK
                     "
     gene            complement(10873..12093)
                     /locus_tag="ENO07_03385"
     CDS             complement(10873..12093)
                     /locus_tag="ENO07_03385"
                     /inference="COORDINATES: protein motif:HMM:PF12679.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDS01040.1"
                     /translation="MKFRNIRTIYGKEMKDTLRDRRTLIFMLIVPIVAIPLLIMFTSS
                     LMVGAVTKAQEERATVVFEGFDNLPADLQDTFKAAPLLTVKTENDYADTIDLIDQLKA
                     GGFDALVSVPDYFGSAIESESPTSIDIFYDQAELKSEFAVDKIRDILEPYEDKIVAAR
                     LQERQMSVEIISPFEVNRQNVASAQKVAGRELGGILPYLIIIMCFMGAMYPAIDLAAG
                     EKERGTLETLLVSPASRGEFVIGKYLVVLTTGIVAALLSMVSLTFSLNYMVNNLLGDI
                     ASELLRLQFNLETVTLIILIILPLAGIFASVLLSVSIFARSFKEAQSYITALNMLVIL
                     PAFVSLLPGIEIDYKMALIPVINVSLIIKNAISGSVEWNYVGVAFISTLVLALLALLF
                     CKKWFERESVIFRM"
     gene            complement(12095..12826)
                     /locus_tag="ENO07_03390"
     CDS             complement(12095..12826)
                     /locus_tag="ENO07_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDS01041.1"
                     /translation="MIEAKNLTKIFKDKKRGEVRAVDDVSFTCKKGEVFGLLGPNGAG
                     KTTTLRLLSTAIRPTSGTALIEGADIVKEPQKVRHVTGFLSGNTGLYGRLTAREMVEY
                     FGKLYGMNKGQIVQRTNQIFDMLDMNEFADSRNEKLSTGMKQKVSIARSVLHDPPVMI
                     FDEPTSGLDVMSSRTIVQFIRQCRDDNKCLIFSTHIMSEAMRLCDRIAIIHKGKIYFE
                     GTVDEALEQTAYDNLEDAFIEIVGV"
     gene            13058..>13845
                     /locus_tag="ENO07_03395"
     CDS             13058..>13845
                     /locus_tag="ENO07_03395"
                     /inference="COORDINATES: protein motif:HMM:PF06452.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01042.1"
                     /translation="MNRLILDKSPLLLTVLLVMIATSPAFSNDWVPVYSPEIVIPRTS
                     GEIMVDGELDDPGWKTAAYIDRFHEHFPGDQTRPPVETAVFVAYDDDNLYVAFVCEDD
                     PSAIRATYGERDNVGWDDNVIISIDTYGDAAWAYELGANAYGIQQDALWSRGGAEDGS
                     FDVIYESEGIITDSGYQVEFAIPFSSLRFPTREAQTWKVDFWRNHPRELRRQSSWAAY
                     NRDESCWPCQWGTVKGIQGVKPGHGIELLPSIVASQSGALDDIE"
BASE COUNT         3467 a         3434 c         3276 g         3668 t
ORIGIN      
        1 attcaacatg agcaagcggt gagtcaatga gtccactgct tctctgataa ggtccattta
       61 aatataaaca tatggatgaa aatgtcaatg taaatgctta tttaattcaa taccatatgg
      121 acttcaagga cttattggtg ccaatgaaaa aaccgcccgg gaatatccgg acggtttttt
      181 gaaaactctg tttctattaa caatcggggt cggtatcccc attggaatcg caaggtgcgt
      241 ttccgcccac aaaaacataa ttgatgatcc agacagcgtc agagacatta acagtcgcat
      301 cacagttggc atcacccgcc tctaacggat cgggggtagt cccgccgacg aatacgtaat
      361 tgattatata aaccgcgtcg gagatattga cggtttcatc ggaattggcg tctccgcaga
      421 tgtacgggag accttcgaaa tgggtgaatt taatgcccga ccaaaagctt tccgcatcat
      481 tctcatcttt ggcctgaact ttaatataat agtcacctgc ctcagaccag ctatgagaag
      541 cagaggcttc cgcgcctgat tcatacggtc ccagccataa ggtggtatct ccgtcacccc
      601 aggaccatcg gtaccataat tgatcctctt ccggatctgt cgtactcgag gtataggtgt
      661 actctgtcat cagattcccc tgagtgaccc cggccggaac tgatggtctg tcgggggcaa
      721 ggttgatgaa ggtgttgaga gttgccgaga catcaacagt aatcgtacct ttggcattgt
      781 aagtcaatga caccgagagg tctatttcgg gcacctgacg tgacccggct aaatccagaa
      841 agttgatata tggggtgaga ttagaatacc ctcccacaag gacggtgtcg ccggcatcga
      901 aataacatgt gggaacggta tgattgttaa gtgaatcgcg cctgggctga acattcggtt
      961 caccggcgca aagagcggca taatagaaat tatatgaacc gccatcataa attgcatcca
     1021 acgcggcccg cgttgatgga cagccgggtc acccgtccca ggtacattcc tccaggaaca
     1081 cagtatgagt atatcctccg gtggtctcat tgtaaccagt ctgtccggga atggctgcgg
     1141 cacatgcgtc agctaaatat gccgtgaaag gatcacccgt atcgccctga tagatataac
     1201 taaattgcgg aaatccttcc atatgattga agatcacggc aattaccatg atattagacc
     1261 cgtcgatagt gcctattccg ttttgggcag gatcccaggt ggccgttttg ttgaagggaa
     1321 cctgataatc cagattaagg gcagtatcga ttgcaaaatc gagaaagccg aattcataat
     1381 tgtgtccgcc ataatccctg tacctcgata caggctcgac aacataaact ttcaactggc
     1441 aggcataaag ggcgcgcccg ctttctccca gggctatttt attatcgccg aaatcaaggg
     1501 cttttgcagg ctgacttgag ataccaggac cataatcata caacctggtt gtcagggccg
     1561 ggtcggccgc aaatgtgacc ccggtgaaaa acaaaaaagc cgcaacgaga gcgaccaggg
     1621 catacctccc gggtatgatt cctttaggga ctgggtgcat agtttttcct cctatgtatt
     1681 agcagtaatt taacattcac aggcattcgg tcagtaatta ttataattta agtaatattt
     1741 acattttggc aatatttttt tcagccagtc tcctaaatat caataccgga caactattgt
     1801 tgtaattgct aagtccattc ttgcgacagg aagaggtttt atgcctcttt gatttttata
     1861 cggatcagat agtttattta cttgataaag ttcaatcgaa tttctatatt agagtcgtat
     1921 aattgatgat gatttttgta tagcacaaac ttgcaggctg ttgtcttccc gggaggtaga
     1981 tatgagattc gccgctatat tgatttccgc agcatttatg atatttgcct tttgcagttt
     2041 cggaagttcg ccccttcgcg ccgatgacgg tgcggaatcg gaagagcttt tcgtcgatct
     2101 tgatggggat ggattcaatg ataacactcc tgacgaggac aataacagca tacctgacag
     2161 cgccgaggat gataaatatt cagaccggaa taaaactgcg aatgccctgg cctccacaga
     2221 atttaagcct attgagggtc ttggggaatt tgatctgagc agcttgttat ccaactctga
     2281 attattcggc cagttgaaat ttttaacccg tgcacaatca tgctgccgcg gcggttttga
     2341 ggccggctca gatttcgggc cgggaaacgg catcggcctg ggggccgtat cgggatgcgc
     2401 cggcggtgta tgtcactgac agtttaatgc agcttatgcg atacctatta attggaatct
     2461 ttattacagt cttttttgta tttccggcat acgctgattt tggctatgag gtcaaactca
     2521 gcggaagcgt tacaaataat ctcatcagtg actcatctga gttatatgac tcttattcca
     2581 ccacaagtgc cgcactgaag tattaccctt ttgcgcagct cgagctcaat ttgaagagcg
     2641 aatacaatta ttactccaat ctcgtgggct tgggaaatat catgggcggc gcggaaatta
     2701 cctggattcc aaccagccag tcatcgcctc tggcgttata tttcaacgcg ggctatgata
     2761 atcgttctta tcgggaatct ttcagtgttt ataacaccaa cgtatataac ggcatgggct
     2821 caatttccta caggctggca agaagggcgc aggttcgagc aggaataaca tatgaacaga
     2881 ccgaattcga caactcggaa ggcgccgaca aggaaatcta tggttttatg ttcggtgcta
     2941 atgtcacatt cctcggttcc aacagcctcg atatcgagag cggctattct tttgctgatt
     3001 ttatttctgt cgatgaagtc tcgataatgt cctggccgca ggttctcgag gaaaccgatg
     3061 gcgacctcaa atcattttat atatcgccaa ggtattcccg cccgctgggc agtaagctgg
     3121 gtatgaactt aacctataca tacctgtcct ttgtggactt tgatgatccg gtcgtataca
     3181 gctactcgac cggctttcta tccccctggg cctcggtctg ggatgggaat tcggttgcac
     3241 tcaatctgaa aagctatcac attccgaaga tgatcatcac tgccggcggc ggctactgga
     3301 acaagagact tttaagaacg ttggagcttc ctgatcggat agtaacacgt gaggatgatt
     3361 acagccgcta ttatatcggg ttgagctggc cgatgccctc cagatcggga gcctatctgg
     3421 aaccgagcat acagctcgac atcattaaca acacctccac tgacaggctt tatgattatt
     3481 cgaccttctc agtagtggtg ggattgacct ttcgaagata gcctgaggaa ttcagaaaat
     3541 ttctaattga cctgctgcgg caggtttttt tatgggcagt catggcacgg tctgggaccg
     3601 ccgacgaata cgaaattgat aatatagata gcgtcggaga cattgatctg ggaatcgcag
     3661 ttggtatccc cagattccag tggctgcggg ggcagtccgc ctgcaaagac gaagttgatg
     3721 atggctacgg catcacttac atttacatta tcgtcagcat tggtatcgcc gcaaaagtaa
     3781 accgtgacag caaagccata actgtctgaa ttgcagtaat catgaacatg cacatctatg
     3841 gattcggtcg aactttcttg gggcgctgta ccggtcaatg tgtcattgat tatcgtacac
     3901 cagtagggat aatcctcata gaatattgta tgcaccgaat catccggatc ctgaatcgca
     3961 ggataataag taaaggtcca cccgtttctg acataaacca gggtcagggt atcgatgatg
     4021 gtcggcggct gattggaatg atcgatttcg atcaaaacca gtgcggaatc agcaagctcc
     4081 ccgtccgaga ctttcacgat gaatgatgtg tccgcataat cacattctgc ggtcccgctg
     4141 attgaatccc cggagacaag gcaccatgat ggaaagttgc tgaaactgat ctcaagctcg
     4201 ctgccgtcgc aatcgggatc ggaagcaatt gctttaaaga caaaatcgcc tccgggttcc
     4261 aggacaattg gcggaggtgc gtcgatcact ggcggatcat tttcgccgat cggattgcag
     4321 caatcgtctt cgggatgagc cgggcagata tcgcatcggt ctccggtacc gtcgccatct
     4381 gcatccgcct gatccggatt cgatatatcc gggcagttgt cggtctggca ggtgtttgca
     4441 gcgtagcccg ggtcaccata accgtcatta tcagtgtccg tgcattcatc acagagatca
     4501 cccaagccgt caccatctgc atccaactga tccgggttat agatctccgg gcagttatct
     4561 tcttcgcaga tatttccggc aaatccggga ttgccgtagc catcgttatc ggtatcggta
     4621 cattcatcgc agatgtcgcc gataccgtcc tgatcataat ccgcctggtc gggatttgcg
     4681 gtatccgggc agttatctat atcgcagctc tggttttcat atccgggatc accgtacccg
     4741 tcaccgtcgg aatccgtaca gtcatcacag acatctccca ggccatcgct atcggcgtca
     4801 gcctgatccg ggttggcaat ctcagggcaa ttatcggtcg cgcagacatt attcggatag
     4861 ccgggatcgc cgtatccatc gccatcgctg tcattgcagt cgtcgcattc attaccaatc
     4921 ccatccaaat cgtcatcaag ttgatcaggg ttaggaatag ttggacaatt atccgtcaca
     4981 caggtattgg cgggaaaacc ggggtcgccg aaaccatcac catctatatc tgtgcaggcg
     5041 tcgcaggaat cccctatatt atcggtatcc atgtcccatt gatagggatt ataatcgtca
     5101 gggcagttgt cgatcatgca tgtgctggcg ggatagccgg ggtcgccgaa gctgtcgccg
     5161 tcggtatcgg tacaataatc acattgatct ccccttccat caccatcggc gtcgtactga
     5221 tagggattga aatgattggg acagttatcc gtgggacagg tgttattggg aaatccgggg
     5281 ttcccgaagc catcgccgtc ggtatcggta cactggtcac attcatcccc gataccgtca
     5341 ccgtcgtaat cctcctgatc cggattgtaa agataggggc agttgtctgt ttcgcagaga
     5401 ttgactgcaa agcccggatt gccgtagcca tctccatccg tgtccacgca ggtatcgcag
     5461 acatagccga taccatcatc gtcataatcg atctgattgg gattataatc attggggcag
     5521 ttatcaaact ggctgcagat attgtcctga tcgggatcgt cgcagtcata atacagcctg
     5581 aaattgtcga tcatccacca cctgtcgttg cgggccaggt aataatgaaa acgcacctgt
     5641 atcgagtctc cgctgcctat gtatggggtg atatcgatat cgcgggtttc cggcccatgg
     5701 ctcaggttgt tcatgcgcag gacgttatgc cattgcccgc ccgcgccgtt acggacatcg
     5761 acgtcagcaa cctcatccgg cgtgtcatta tcgtacgaga agtagtttga gaaacgcaga
     5821 tgaatattaa tatctgaagg ggcatggtaa aatgcggtca ccacagcctc gtccatatag
     5881 gcgttgggcg atgtgctgga attgactatc aggcagctat cctcgaaggg cgggtaaccc
     5941 ccaaggctgt acatatccat aaactgccag gtataaccgt cctgaaaccc atccagaatc
     6001 acccagccgg ggggcagttc accgttgtca aagtcataat atatcagtgt gggcataaac
     6061 atgctgtcgg gttccgacgc cggcgccgac tgagcatatg cggattcggc cacataagca
     6121 aagagcagaa atataagaaa caagggggcg aactttctca tattatcccg cgaatgttta
     6181 tgttctaaac ttatctgtgt gcagtcccaa aacttggggt catttataaa atagccccgg
     6241 acggctaatt tgtcaacatt aatcatgcaa ccatctgctg atggcccggt tttattgaca
     6301 tattaaggat gaccgcatta tttatgatga tcggaatatt tgtgaatttg atgagagcgc
     6361 cgaaataaaa agcgggagcg attattttta atatcgctcc cgctaaattt tatagtcttg
     6421 cgtctatctc tttttgcgtc ttcggcttac aaaatcccag aagacataat ctccgaggtt
     6481 ctgagacgag gcaaaataag ctcggccgcg attcaattcg gtcagcttgg ccacaaattt
     6541 ctgcaggtag ggatcgtcgg ttatcataaa ggtggtaatt ggtatgcgtt ttttgcggca
     6601 aattatggct tcatccagcg tccggttcac aattaccggg tcgagtccca taggattttt
     6661 gtaaaccgag ccgttccggc gggttatcat ggatggcttg ccatctgtga tcatgaatat
     6721 ctgcttattc acatttttct ttttgagcag aatgttgcgc gccatctcga ggccggcttt
     6781 ggtatttgta tgatatggtc ccacaccgat atagggaatg tccttgacct tgacctctct
     6841 ggcaaaatct ccaaaaagaa caatattgag actgtccttg ggatatttcg tgagtatcaa
     6901 ttcagagaag gccagcgcca cctgcttggc cggggtaatc ctatcctcac catacagcac
     6961 catggaatgg gatatatcga tcaacataac agtggcgcag ttagtcagct gctcggtatc
     7021 ataaacctcc agatcttttt cggccatatt cagacccagg gagccggtgc gcctgattgt
     7081 attcatcagt gaattctgga aatctatgtg attgaattcg tcgccaaacc tgtattctct
     7141 cttttccggc agggcctctt ccgagctgcc cccgccgtga gggattatat ggccgccggc
     7201 tccggatttt cggagatgtt caaatatact gtcgaaagca tcctgcctga gcgacttctc
     7261 gccctttcgg gttaatgata tgttatcccc ctgccaggag acgatattgc tcttttccag
     7321 attcttctcg aaaccatcga gatcaaaatc ctcggggagc acccccattg cctgaagctt
     7381 gcgcatcagc ttgagagcca tctcggcatc accgttgacc tggagcagaa ggtagttgaa
     7441 gatcgacatc aggtccttga gattctggag tttctgtata gccgcgtcat cccattcgga
     7501 atatataaaa cgcataatca gctcctgagt attcgctgta tcatatcggt atagatgaac
     7561 ttattgtcgg gcgattcctt ggcaatccgg ttcatctgat gcagcccctc cagcacaaat
     7621 tccatatacg taaatatctc atcttcctcc ccgagatcgc aatacttcaa gacaatctcc
     7681 ctcagtccta caatgtccga aagagctttt ttgtatttcg aataaggcat ttcatccgat
     7741 atctcaattt gttttccggc cgagaagaaa tcgattaccg gctcgtaaac gctgtcttcg
     7801 gggatgattt cctcataatc gccatcggga gtgcgcttgc gctcttttcg cggctccggg
     7861 aaaatgcgca ggaaggtctg tttgattgcc tcacccacaa ggttcacagc caccatcgag
     7921 ggcccctcct gctcgccctc atagaccagc tcaatcttgc ccataatcga gggcaggacc
     7981 gcatacaggt cggcaatacg gggatgcgca tgcttatcgt tattcatcac aacgcgcctc
     8041 tcaacgttag agagcagatt ctcataggca gagattgtca aacgggccga tacgcccgaa
     8101 gtctgatcta catactcgct ctcacgggcg cggaacgcta tctcctccac gatatctttt
     8161 atcaggtcgg ggattttgat ctccaccccg cgctctttcc aggcttcctg ttcggtaatc
     8221 tgtttggcct catccaggtc acggggataa tgggtgttga cctgggaggc aattctatcc
     8281 ttgagaggag taattatgtt tcctcgattg gtatagtcct ccgggttggc tgtgaaggcc
     8341 agcatcatat ccagcggtat ccggaccgga aagcccctga tctgcaggtc attttcctcg
     8401 agaatattca agagggccac ctgaatcctg ggctgcaggt cgggaagctc atttatggca
     8461 aatatccccc ggttggtgcg cggtacgata ccccagtgga tcacctcttc gttggatata
     8521 tccagcttct cccgaaccgc tttaataggg tcgagatcgc caatcaggtc ggccacagtt
     8581 acatccggcg ttgccagctt ttcgttatag cgctgttcgc gatgtatcca gtcgatttcc
     8641 agctcgtccc ccgcttcagc cgccagtctc ttatgtttgt ccgagatcgg tttaaatgga
     8701 tcttcgttca actggctgcc ctttattacc ggaagatact catccaaaag tgaccccagt
     8761 tgacgtaaaa ttcgtgtttt ggcctgcccc ctcaggccca gaagcaatat atcatgtcgt
     8821 gagagtatgg cgttttccaa ctggggcaaa acagttcgat cgtatcctat aatacccgga
     8881 aagcgttttt tgccggattt tatcaaatct atcaggtttt ctcttaattc atccttggtg
     8941 gaacgagacc tgtatccgga ttccttgagt tcgcccaatg tctttgcaaa tttcatattt
     9001 cccttccaaa ttcaatttag agttcaataa atcttaacaa atttagtctt attgggttca
     9061 aaagaaaatt cgggtaaaat tgaacatttg tggagtcatt acgtctataa aaacagcatc
     9121 ttcggattca agctgatttt gaaatctatc ggattttaaa atgtcccttg caagggagga
     9181 aacatgcatt taacgtccct catatcaatc ttgcttgcga tcaaagccct gtttgataac
     9241 atgaacctgg atcatctgcc gttcttctaa gaaaccgcgg atgaaattcc tccaaaaaga
     9301 aaggcgcaca tccaaagaat gtacgccgga tgggaattga aatggggagg aaaaattatt
     9361 caaattcctg tgctatttgt cttttggaga tcgatcaata aagccctgtt tatatatccc
     9421 caggatgccg ataacctcgc ctgattcatc ctcttcgaat ttcatcttga aataatcgac
     9481 tccctcgatc atgaaaatat cctctgatag agcgatcagc ttctgtttgg gacggtcgtc
     9541 cctctggtaa tagagctcat ccccctccat ccagatcttt ctggggccgt aatctccgac
     9601 atatttattc attagctctt ccggaacctc gaccggctcg agctgtgcct tgagcatatc
     9661 cagatcccat cgaagcatac ccttttcttc ctcactttca gctttggcga ccagcttctc
     9721 catagcatcc acccgagcca cttccagggc atggtcggcc gcacacgaga tatgaggttc
     9781 aacccctgtc ccttcccaat tcgttccggt aattggattt atggcgcgtc cgaagggcac
     9841 acgcaaactt actttgagat tgggaaatat ttcgccctga acgggatgcg ccccgccgcc
     9901 ggtggtttcg cccacaatcg tggcgcgttc gagattcttg agattatagg taaattcctc
     9961 agcggcagag aaagtatatc cgctcgtgag aatgtaaatt ggagtattgg ccatacgcgg
    10021 cccctcgaca tgggaagcgg tccagaactg ctttgtagta tcctctttgc gaatatagaa
    10081 gctgttcaga tgtaccgggt cgttaaaaaa ataactcgat ataagctgta tcatcgaggg
    10141 agaaccgccc ccgttttccc tcaaatcgat aatcagggcg tccacgtggg ccaggaaatt
    10201 catggcggca atagcggtag ccccgccgaa atatgcatca acaaatccat tgaatttcaa
    10261 gtagccaatg tttcctggaa gtatcctgat ctccttgaaa ccgaagttgt ccctcgacag
    10321 ctcttcgaaa tgttcctgtc tttcctcatc cgttacagtg tcttcttcgg tgcggatatc
    10381 atccatagta atatcgggcg tataacgtac gcccaggtgc cggtcatggc agatatcacg
    10441 caggtccttg gtgagcctat ccgaaaaggc gctcaggctg gtaatatctt tatattcacc
    10501 tttctgatac atccccttca catgcaccgc catctcttcg gccacatccg gaaacacata
    10561 gacttcgttg attacccggg ttacagaatc gataatctcg gcctgagtct ttgagtcgat
    10621 tgtctcatcc cgcggctcgg ccctctgcag ctgaccgtat agcggcataa tcatactcgt
    10681 gaccagtaac aaaataagtc ccccgaatgc tttcctcatc ttcatcctcc cttgttagat
    10741 tctcttttgc cggcattgtt tcaaaatcaa ttcacttata cgaatctgat tttaaaaggt
    10801 tcaataatac gtaagcgctt acatataata ataaaataaa tcagggtggc aacaattgaa
    10861 attataaatc tattacatgc ggaagatgac tgattcgcgc tcgaaccatt tcttacagaa
    10921 cagcagcgca agcagggcca gcaccagggt agagatgaag gccaccccga cataattcca
    10981 ttccacgctg ccggaaattg cattcttgat tataagagaa acattgataa ccgggatcag
    11041 ggccatcttg tagtcgattt caattcccgg aagcaatgag acaaaagccg gcaggattac
    11101 cagcatattt agggcggtta tatagctctg ggcctcttta aatgaccgtg caaagataga
    11161 gaccgacaac aagacgctgg cgaagatacc cgccagcggc aatatgatca gaataatcag
    11221 tgtcaccgtt tcgaggttga actgcagcct cagcaattca gaggcgatgt ccccaaggag
    11281 gttattgacc atgtaattca gcgaaaatgt caggctgacc atggaaagca gggccgccac
    11341 aataccggtg gtaagcacta ccaggtattt gccgatcaca aattcgcccc tggaagcggg
    11401 cgataccaga agggtctcca gcgtccccct ctccttctcc cctgccgcga gatcgatggc
    11461 cgggtacatg gcgcccatga agcacatgat aatgatcaga tagggcagga tgccgcccag
    11521 ttccctgccg gccactttct gtgccgaggc gacgttctgg cggttgacct cgaaggggct
    11581 gataatctcg acagacatct ggcgctcctg cagacgggcc gccacaatct tatcctcgta
    11641 cggctcgagt atatccctga tcttatcaac agcaaattcg gatttcagct cagcctgatc
    11701 gtagaagata tcgatgctgg tgggggattc gctttcaatc gcgctgccga aataatccgg
    11761 cacagagacc agtgcatcaa atccgcccgc cttaagctgg tcgatcaggt cgatagtgtc
    11821 ggcataatca ttttcggtct tcaccgtgag cagcggcgca gccttaaatg tgtcctgaag
    11881 atcggcggga agattatcga atccctcgaa aacaactgtc gccctctcct cctgggcctt
    11941 ggttaccgcg ccaaccatca gcgaggaggt aaacattatc agcaggggta tggccacgat
    12001 cggcacaatg agcataaaga taagtgtgcg ccggtcacgc agagtgtcct tcatctcctt
    12061 gccgtatatg gtgcggatat tcctgaactt catcttatac tccgactatt tcaatgaagg
    12121 catcctcgag attatcatag gcggtctgct cgagcgcctc atccacggta ccctcaaaat
    12181 agattttgcc cttgtgaatg atggcaatgc gatcacacag ccgcatagcc tcactcatga
    12241 tatgcgtgga aaagatcagg cacttattgt catcccggca ctgcctgatg aactgcacta
    12301 ttgtacgcga actcataaca tccagtccgc tggtaggctc atcaaagatc ataaccggag
    12361 gatcatgcaa aacagaacga gcaatcgaga ccttctgctt cattccggtc gagagcttct
    12421 cattgcggga atcggcaaat tcattcatgt cgagcatgtc aaatatctga tttgtgcgct
    12481 gcacaatctg ccccttattc atcccgtaaa gttttccaaa gtattcaacc atctcccggg
    12541 cggtcagcct gccatagagg cccgtgttac cggatagaaa tccggttaca tgtctcactt
    12601 tctgaggctc tttgactatg tccgcgccct cgattagagc ggtaccggag gtgggcctga
    12661 tcgctgtgga gagcaaacgc aaagtcgtgg tcttacccgc gccattgggt cccagaagtc
    12721 cgaatacctc tccctttttg caggtaaatg agacatcgtc gacggccctg acttcacctc
    12781 gttttttgtc cttgaatatc tttgtaagat tttttgcttc tatcatacca cctcaatgaa
    12841 tgcaaaagta tatgtcttcg aggacatttt tgcaagtctt ttgatgaaat gcatacaaac
    12901 atatcatgcg gatattcgat tcttcttcaa gatttctacc ggagaacagc ccgacaaaat
    12961 agtgtaccga tttagtctga cattaacaaa tgtctcatat gaaagcagca ataatatttc
    13021 ctgaaacaga tatgtagttt ttccgtatgc ctgaaatatg aatcgcctga tactggataa
    13081 aagcccatta ttattgacag tgctgctagt gatgatcgcc acgtctccgg cattttcaaa
    13141 tgattgggtg cccgtctatt ccccggagat agtgatcccg agaacttccg gagaaattat
    13201 ggtagatggg gaacttgatg atccgggatg gaaaaccgca gcctatatcg acagattcca
    13261 tgaacatttc cccggagatc agaccaggcc cccggtcgaa accgcggtat ttgttgccta
    13321 tgatgatgac aacctgtatg tcgcttttgt gtgcgaggat gacccttctg ctatccgggc
    13381 aacctacggc gagagggata atgtcggctg ggatgataac gttattattt ccatagacac
    13441 ctacggggat gccgcctggg cttatgagct gggagcaaat gcctacggta ttcagcagga
    13501 tgctctctgg tccaggggcg gggccgaaga cggcagtttt gatgtgatct atgaatcaga
    13561 aggaataatc acggattccg gctatcaggt ggaattcgcc atacctttct catcgctcag
    13621 gttcccgacc cgtgaggcgc aaacgtggaa agttgatttc tggagaaacc atccccgcga
    13681 gctgcgcaga cagagctcct gggcggcata caatcgtgat gaatcctgct ggccctgcca
    13741 gtggggaaca gtcaagggaa tccagggggt caagccgggg cacggtatcg agcttctgcc
    13801 ctcgattgtt gccagccaga gcggagctct tgatgatatt gaaaa
//
LOCUS       DSEB01000186            4721 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001500, whole genome shotgun sequence.
ACCESSION   DSEB01000186 DSEB01000000
VERSION     DSEB01000186.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001500"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            235..1755
                     /gene="accC"
                     /locus_tag="ENO07_03400"
     CDS             235..1755
                     /gene="accC"
                     /locus_tag="ENO07_03400"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDS01043.1"
                     /translation="MIKKILIANRGEIAVRVIRACREMGITNVAVFSDIDSVAKHVRM
                     ADEAYHIGPSPSSESYLVMEKIIEVAKKAGVDAIHPGYGFLSENARFAKMVMDNKLTW
                     IGPPPEAIARMGDKLEARVTAVDSGAPVVPGFKSKIENVEQIIDEVHKVGFPILIKAA
                     AGGGGKGMRIVNNEEELPKALKTAANEARSAFGDDRVYFEKYIPKPHHVEIQILADKF
                     GNMVYLGERECSIQRRHQKVIEEAPSPIITPEIRKKMGEAALKIASACGYVNAGTVEF
                     LVDDDLNFYFLEVNTRLQVEHPVTEMITGIDLVKEQIKIASGEKLSFKQDGIRIHGHA
                     IECRIYAEDPAANFAPSTGTLRSYVEPHGVGVRVDSGVYEGAQIPIFYDPMISKMIGW
                     GATREEAVARTRRALTEYRISGVCTTVNFHLAVMDNENFKKGKINTHFIEEQFPDNVF
                     SSKEEDHVYKAAAVSACLYDFLENSKLKTRVSSDGGRESNWKIAGRRMGVSRTPEK"
     gene            1758..2276
                     /locus_tag="ENO07_03405"
     CDS             1758..2276
                     /locus_tag="ENO07_03405"
                     /inference="COORDINATES: protein motif:HMM:PF00364.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HDS01044.1"
                     /translation="MDYQVKIEDREYIVTIDPSTHPPQIKVDGVPVETKFSTRSDNSE
                     IQLLMDNRSYDIEVVKQKGRQNGDFSVFIYGREFSLYAEDERLAKIREVAGLGLGGDT
                     RKELHAPMPGLVTKVLKSPGDQVSRGESLIIVEAMKMENELKSLIDGTIKEIKVKEGQ
                     SVDKNALMVLFE"
     gene            2273..2863
                     /locus_tag="ENO07_03410"
     CDS             2273..2863
                     /locus_tag="ENO07_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01045.1"
                     /translation="MNAKAFSRLSVGATYRFDIKKALFSQAGALFALVMAIISEGNPP
                     FGDEPYRLFSPVTFFLAVNVAVLVYTLIALGSSWIKLYPSMMIYTSRGDQTRIDFARV
                     TRVVRQSNKELSFYITDHAGKETLGLKIKNYAAYANSEEMLTFLKYLLGDKFSGHIKP
                     EEGQEIDKDKTKAKEDLPPARFKIPNRFFRDKSRNK"
     gene            2875..3279
                     /gene="mce"
                     /locus_tag="ENO07_03415"
     CDS             2875..3279
                     /gene="mce"
                     /locus_tag="ENO07_03415"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015849669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HDS01046.1"
                     /translation="MIEKIAHIGIAVRDIEKHLSLYRDVMGMKVGERVKVPDQGVEVV
                     FLETGDTKIELLQPLNEDANINKFLEKRGEGQHHICLEVDDIEAELRKLEENGIQLID
                     KKPRKGACGEMVAFLHPKSTGGVLIELEQKKK"
     gene            complement(3954..>4721)
                     /locus_tag="ENO07_03420"
     CDS             complement(3954..>4721)
                     /locus_tag="ENO07_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HDS01047.1"
                     /translation="IYARRMREKYGAKDPRSWMLRFHTQTAGCSLTAQQPELNIVRTA
                     YEAMSAALGGTQSLHTNSMDETLALPSDKAALIALRTQQVLAHETGVVNTIDPLAGSY
                     FVEGLTNRIEKQALEYFDEIDKRGGVLKGIETGYFQREIARAAYQFQQEIDKKERIIV
                     GVNDFILEDEEIEIEILKIDEKAHLEQMESIKKAKAMRDNDKVARTLEELKKVAATPS
                     DNTMPYIIDAVKAYATMGEICDVFREVYGEYVENPEI"
BASE COUNT         1284 a         1110 c         1193 g         1134 t
ORIGIN      
        1 aataaatcgt aacagcgacc cgttcttcgc aaaaactatt gccaaataca gcttttatgc
       61 ataatttgaa aggttgtagg ttaaatcctg aaggctaaag gtccggctct tgacggggat
      121 ttgacaatca cgataatgta gggcaggttc cctgtgaacc tgccatcgcc gataaattta
      181 tatttggcag gagcacgggg ctcctgccct acggatacgg gttttgatac aactatgatc
      241 aaaaaaatac tgattgcaaa tcggggcgag attgcggtga gggtgatacg ggcctgccgt
      301 gagatgggaa tcaccaacgt agctgttttc tccgatatcg attcggtggc caagcatgtg
      361 cgcatggccg acgaggccta tcatatcggg ccgtcgccgt catcggaaag ctacctggtg
      421 atggaaaaga taatcgaggt ggccaaaaag gcgggcgtgg atgcgattca tcccgggtac
      481 ggattccttt cggaaaatgc cagattcgca aagatggtca tggacaacaa gctgacctgg
      541 atcggccctc ctccggaagc tatcgcacgt atgggcgata aactcgaggc aagggtgacg
      601 gctgtcgatt cgggagcacc ggtagtgccc ggctttaagt cgaagatcga aaatgtcgag
      661 cagataattg acgaggtcca caaggtcgga ttcccgattc tgattaaagc ggctgccggc
      721 ggcggcggca aggggatgcg gattgtcaat aacgaagaag agcttcccaa agcattaaag
      781 acagccgcca acgaggcccg ttcagctttc ggcgatgacc gcgtgtattt cgagaaatat
      841 atccccaaac cgcatcatgt ggaaatacaa atccttgctg ataaattcgg aaacatggtt
      901 taccttggcg aacgtgaatg ttccatccag cgcagacatc agaaagtaat cgaggaggcc
      961 ccctccccta tcatcacccc cgagattcga aaaaaaatgg gcgaggctgc attgaaaatt
     1021 gcaagcgcct gcggatatgt caacgccgga acagtcgaat ttcttgtcga tgacgacctc
     1081 aatttctatt ttctcgaagt caacacccgc ctgcaggtgg aacatccggt caccgagatg
     1141 atcaccggaa tcgacctcgt caaagagcaa atcaagattg catccggtga aaagctgtca
     1201 ttcaagcagg atggtatccg tattcatggc catgcaatcg aatgccggat ttatgccgag
     1261 gatccagccg ccaactttgc gccctcgacc ggcacgctgc gaagctatgt tgagccgcac
     1321 ggcgtcggag tacgtgtgga ttccggagtt tacgagggcg cgcagattcc gatattttac
     1381 gatccaatga tctccaagat gataggctgg ggcgcaactc gcgaagaggc ggttgcacgc
     1441 acaagacgtg ccttgaccga gtatcgaatt tccggcgtat gcacaacggt gaatttccac
     1501 ctggcggtaa tggataacga aaatttcaag aagggcaaaa tcaataccca ttttatagag
     1561 gaacaattcc cggacaatgt tttttcaagc aaagaggaag atcatgttta caaagcggcg
     1621 gcggtctcag cctgcctgta cgattttctt gaaaacagca agctcaaaac ccgggtctcg
     1681 agtgacgggg gtcgggaaag caactggaaa attgcaggta gacgtatggg tgtcagccgc
     1741 acaccggaga aataaatatg gattaccagg ttaaaataga agacagagaa tacattgtaa
     1801 cgatcgatcc ttcaactcat ccgccccaga tcaaagtcga tggagtgccg gtggagacca
     1861 aattttcgac ccgttcggac aattccgaga ttcagcttct gatggataac cgttcctacg
     1921 acattgaagt tgtcaaacag aaaggcagac agaacggcga tttcagcgta tttatttatg
     1981 gacgcgagtt ttcgctctat gccgaggatg aacgtctggc aaaaatccgc gaggtggccg
     2041 gcctgggcct gggcggcgat accaggaagg aactgcacgc gcctatgccc ggtttagtaa
     2101 caaaggtact gaaaagtccc ggcgaccagg tctccagggg cgaaagcctc attattgtcg
     2161 aggccatgaa aatggaaaac gagctaaaat ccctgattga cgggacgatt aaagagataa
     2221 aggtcaagga agggcaatcg gttgacaaaa acgctttaat ggttctcttc gaatgaacgc
     2281 aaaagctttc agccgtcttt cagtcggcgc aacataccgc ttcgatatca agaaagcatt
     2341 attctcgcag gccggggcgc tgtttgcgct ggtgatggca atcatttccg agggcaatcc
     2401 gccttttggt gatgagccct atcggctctt ctctccagtg accttcttcc ttgcggtcaa
     2461 tgtggccgtt ctcgtatata ccctcatcgc cctcggttca agctggatca agctctatcc
     2521 cagcatgatg atctacacct cgcgaggcga tcagaccaga atcgattttg cgcgggtcac
     2581 ccgtgtggtc aggcagtcca acaaggaatt gtccttctat atcaccgatc atgccggcaa
     2641 agaaacgctg ggactgaaaa taaaaaatta tgcggcttat gcaaattccg aggagatgct
     2701 gacttttttg aaatacttgc tgggagacaa attttccggg catataaagc ctgaggaggg
     2761 ccaggaaatc gacaaagata aaaccaaagc aaaggaagat ctgccgcccg ccagattcaa
     2821 aattcccaac cgtttcttca gggataaatc gagaaataag taggagccgg atggatgata
     2881 gaaaaaattg cgcatatagg aattgcagtc agggatatcg aaaagcacct atctctctac
     2941 cgcgatgtta tgggtatgaa agttggggaa cgtgtgaagg tgccggacca gggtgtggag
     3001 gttgtttttc ttgagacggg tgatacaaag attgaattgc ttcaaccatt gaatgaagat
     3061 gctaacataa ataaatttct ggaaaagcgt ggcgagggac aacatcacat ctgccttgaa
     3121 gttgacgaca ttgaagcaga acttcgaaag ctcgaagaaa acggaattca attgatcgac
     3181 aagaaaccgc gcaagggcgc ctgcggtgag atggtggcgt ttctgcatcc gaaatcgacc
     3241 ggcggtgttt taatagagct ggaacagaag aaaaaataat cttaatgaat tatatttggg
     3301 tgacccaatc tttagattgg gcgtgccccc tatttaatat cccaagctga agctcggggc
     3361 acccgaccat acttggagat caaaaatgga agaactattt atatttttgg ctttcttgta
     3421 cttatatatt tgggttatat tgtaacaata gatggtattc agtacctggt taaaaaaaga
     3481 aattattagg cattggtgat ccaagaattt ttcttccagc tggcgttgtc ctgatcttca
     3541 taggtatgtt agctctaatt ggagaaatat taattccatt gtttcaacaa ttattttagt
     3601 aaagagggcg gattatctct ttaagatggg attatcaatc aggaaaaatg agaaacccac
     3661 cgctaaagcg gcgggccaac caaaaattgt gcgcggcgta tgtcctcatg cgccgcggtc
     3721 acatgcgctg aaaggtggac cataacgatg ttgcggcaga tcctcagatc tgccgcccgt
     3781 caggtctgag gacctgacgg aacaagaata tttgggtgac ccaatcttta gattgggcgt
     3841 gccccccatt taatatccca agctgaagct tgggggaccc ataatctaat ctcccaaaaa
     3901 aataccgccg ggaaaggctt cccggcggca cattatacag accctaattt ttgttaaatc
     3961 tccggattct cgacatactc gccgtaaacc tcgcggaaga catcgcaaat ctcgcccata
     4021 gtggcgtaag ccttgacagc atcaataata tacggcatgg tattatccga aggcgtcgcg
     4081 gcgacctttt tcagctcttc cagagtgcgg gcaactttgt cattgtcacg catcgctttc
     4141 gctttcttga tgctttccat ctgctccaga tgcgccttct cgtcaatctt cagaatctca
     4201 atctcgattt cctcatcctc gagaatgaag tcattgacgc cgactattat ccgttccttc
     4261 ttgtcgatct cctgctggaa ctgataagcg gcccgtgcaa tttccctctg gaaataaccg
     4321 gtctctattc ctttcagaac tccaccgcgt ttatcgatct catcgaaata ttccagcgcc
     4381 tgtttttcaa tcctgttggt gagaccttct acaaaatatg aaccggccag gggatcgatc
     4441 gtatttacaa ccccggtttc atgagccagc acctgctggg tacgaagcgc aatcagggcg
     4501 gccttatccg agggcagcgc cagggtttca tccatcgaat tagtgtgcag gctctgtgtt
     4561 ccgcccaatg cggcggacat tgcctcgtaa gctgtgcgta cgatattcaa ctccggctgt
     4621 tgcgcggtca atgaacatcc cgcggtctgc gtatggaacc gcagcatcca gctcctggga
     4681 tctttggcgc catatttttc acgcatacgg cgggcataaa t
//
LOCUS       DSEB01000187            2679 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1005297, whole genome shotgun sequence.
ACCESSION   DSEB01000187 DSEB01000000
VERSION     DSEB01000187.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1005297"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..676
                     /locus_tag="ENO07_03425"
     CDS             <1..676
                     /locus_tag="ENO07_03425"
                     /inference="COORDINATES: protein motif:HMM:PF01523.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDS01048.1"
                     /translation="PAAVANLLEWMNFIAFGSKAFQEETSCLAGRIGEQIMGSNISIF
                     DDALNPAGVPFPFDLEGVAKKGVEFVADGVARGVVYDTISGNKENKQSTGHALMPSES
                     GEGAMPLNVFIKPGDSSLEQMISNVEKGILVTRFHYINGFLDTRKALMTGMTRDGTFL
                     INNGKLSNGIKNLRFTDSMLDVFSNVAEISSESVSIPGWWDAMGCNHVPAMRVSEFNF
                     SGKTDF"
     gene            789..>2679
                     /locus_tag="ENO07_03430"
     CDS             789..>2679
                     /locus_tag="ENO07_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01049.1"
                     /translation="MNTFERQHGRSGIKTAALLLLALICFGPVTLHGASYTIFNMQDF
                     SSQELRMQAFSLNDDSELEIDIIGARHRDSDQMYALGWILDSKTREVVWSMSEEATRR
                     LEEDRKLRRYNGTIYLDRGEYEAYYFAGNPLQMLTDFHLKGDIEGLGDLLGIVGDALD
                     SEFSKDGTIYSNQMKRFKFQLASFENNFRVKGDEPPVLPNQVISLVRPRNLSDERAVF
                     MVTGSAFVRVYAIGEYSESNDVFMDGAKIINARTREKVWAMERWNTDWGGGALKNRCF
                     HDEIRLEPGDYIVYYWTDESHTFDDWNGTPPYDPFFYGVTLAASSSEIADRIKPSDID
                     ERKKTIVQITKVTDDVNLARDFQLKRDMTVNVYAIGEGRKGYMYDYGWIKNAENDETV
                     WIMREDQTDHAGGAAKNRVFDGILELERGNYTLRYKTDGSHSYGSWNSAQPSDSRNYG
                     ITLFAYEEDFNPDLVEVGPLKVLGGPSETTTTYEFQFQVPPAPDPQKIRELMEQQREI
                     TRVYLDELSDLRRELRESAKDLENIEELRQDMEELKKEYAEDMKELKAEFDREQIEFE
                     NKELEIQMKMLRQELDNLDNKNFIEVHPSSESSDFIVRIGGVGNNANLKTTFKLDRPN
                     RVRIHA"
BASE COUNT          730 a          570 c          721 g          658 t
ORIGIN      
        1 gcctgctgct gtggcaaatc tgctggaatg gatgaatttt atagctttcg gatcgaaggc
       61 atttcaggag gagacctcct gtctggcggg ccgtatcggc gaacaaatta tgggatcgaa
      121 tatcagtata ttcgatgacg ccttgaatcc cgccggggta cccttcccgt ttgatctcga
      181 aggcgtggcc aagaaggggg tggaatttgt agccgacggt gtggccaggg gcgttgtata
      241 tgataccatt tccggtaata aagagaacaa gcagtctacc ggacatgcac ttatgccgag
      301 tgagtcgggc gaaggggcca tgccgttgaa tgtatttatc aagcccggtg attcatcgct
      361 ggagcagatg atttccaatg tcgagaaggg catactggtt accaggttcc attatatcaa
      421 tggatttctg gatacgcgca aagccttgat gaccggcatg acccgtgacg gcacgttttt
      481 gatcaataac ggcaagctca gcaatggaat aaaaaacctg agatttacgg acagcatgct
      541 cgatgttttc tccaatgtgg ctgaaatatc gagtgaatct gtgtctattc ccggatggtg
      601 ggatgctatg ggctgcaatc atgttccggc aatgcgcgtc tcggagttca atttctcggg
      661 aaaaacagat ttctgaccat tccgataaag tattattatg caagtattta gcatgattac
      721 gacgagcccg tgaaacatat cgtcttcaat cccgtatcct tttataagga atggatggga
      781 attgaaaaat gaataccttt gaacgacaac atggcaggtc aggcatcaaa acggcagctc
      841 tgctgctttt ggctctcata tgttttgggc cagtgacgct gcatggggca tcatatacaa
      901 tcttcaatat gcaggatttt tcatcccagg aactgcgcat gcaggccttc agtcttaatg
      961 acgattccga acttgagatc gatattattg gtgcgaggca tagagattca gaccagatgt
     1021 acgctctggg atggattctg gattcaaaaa cccgtgaggt cgtctggtca atgtctgaag
     1081 aggctaccag gcgtttggaa gaggaccgca aactgcgccg ttataacggc accatatatc
     1141 tggacagggg ggaatacgag gcatactatt tcgctggaaa tccattgcag atgcttacgg
     1201 attttcacct gaaaggggat attgaaggcc tgggcgatct actgggaatt gtgggagacg
     1261 ccctggatag tgaattctcg aaagacggca caatatattc taatcagatg aagagattta
     1321 aatttcaact ggcttccttt gagaataatt tcagggttaa aggtgatgag ccaccggtac
     1381 ttccaaacca ggtcatttcg cttgtgcgtc cgagaaatct cagtgatgaa cgggccgttt
     1441 ttatggtgac cggctctgcc ttcgtgaggg tctatgcgat tggagagtat tctgagagca
     1501 atgacgtttt catggatggc gccaagatca tcaacgcccg cacgcgcgag aaggtctggg
     1561 cgatggaaag atggaatacc gactggggcg gaggggcgct gaagaatcgc tgttttcacg
     1621 acgagatcag gctggaaccg ggcgattata ttgtctatta ctggactgat gaatcgcata
     1681 cattcgatga ctggaacggt accccgccct atgacccgtt tttctatggc gtaaccttag
     1741 cggcatcatc atccgagatc gccgatcgta tcaagccctc ggacatcgat gaacgcaaga
     1801 agacgattgt gcagattacc aaagttacag atgatgttaa ccttgcccgt gatttccagc
     1861 tcaagcgtga tatgacggtc aatgtctatg ccataggcga gggcaggaaa ggttatatgt
     1921 atgactatgg ctggatcaag aatgccgaga atgacgagac cgtgtggatc atgcgtgagg
     1981 atcaaacaga tcatgccgga ggggccgcaa agaatcgtgt tttcgacggg attctggaac
     2041 tggagcgggg aaattatacc ctgcgctata agacagatgg atcgcactcc tacggatcat
     2101 ggaattccgc ccagccttcc gacagccgta attatggcat taccctcttt gcctatgaag
     2161 aagattttaa tcctgatctg gtcgaggtag gtccattaaa ggtactgggc gggcccagcg
     2221 aaaccacgac tacgtatgaa tttcaatttc aggttccacc cgcccctgac cctcaaaaaa
     2281 tccgtgaact gatggaacag cagagggaaa ttacacgcgt gtacctggat gaattgagtg
     2341 atctgcgcag agaattgcgg gagtctgcca aagacctgga aaatatcgag gaattaaggc
     2401 aggacatgga ggagctgaag aaagaatatg cggaagatat gaaagaactc aaggccgaat
     2461 tcgaccgcga acagatagaa tttgaaaata aagagctgga gatccagatg aagatgctcc
     2521 ggcaagagct ggataatctg gataataaga attttattga agtgcatccc tcgagcgagt
     2581 cttcggactt tattgtccgc atcgggggag tcggtaataa tgccaacctc aaaaccacat
     2641 tcaaactcga taggccgaat cgtgtcagaa tccatgccc
//
LOCUS       DSEB01000188            3870 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002329, whole genome shotgun sequence.
ACCESSION   DSEB01000188 DSEB01000000
VERSION     DSEB01000188.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002329"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            19..657
                     /locus_tag="ENO07_03435"
     CDS             19..657
                     /locus_tag="ENO07_03435"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HDS01050.1"
                     /translation="MDKYYEERIRMVENQIKRRGIEDSAVLEAMRSVPRHLFVPEAYR
                     PRAYEDGPLPIGNNQTISQPYIVALMTELLDLQPGERVLEVGTGSGYQAAVLAEIADS
                     VYTIEIVKPLADRAGEILDSIGYNNIKVMHGDGYQGYEEAAPYDAIIVTAAPDHVPQP
                     LIDQLAMGGRLVIPVGSIYQYLTLYEKTEDGLKKKTGIAVRFVPMTGKAQEK"
     gene            912..1301
                     /locus_tag="ENO07_03440"
     CDS             912..1301
                     /locus_tag="ENO07_03440"
                     /inference="COORDINATES: protein motif:HMM:PF04024.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HDS01051.1"
                     /translation="MKKLYRSRESNIIAGVCGGFGEYFNIDPVIVRIIFLLLIPAGGT
                     GIIAYIIAWIIVPLRPVNEVAQPPSKEQSEAMMKYLPGLALIIIGAAFLITRIWGWLS
                     FGHIWPAALIVIGAFLIYRAVGSKKEQ"
     gene            1303..2208
                     /locus_tag="ENO07_03445"
     CDS             1303..2208
                     /locus_tag="ENO07_03445"
                     /inference="COORDINATES: protein motif:HMM:PF17115.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01052.1"
                     /translation="MHAGSLNWGLIFIIVGLVALGWTTGRLPAEVFMRLWDLWPVLLI
                     AIGIQMIFSKTKLAGFAYLSSILIILAAAYAVSPYWQQVTTGESLERESGTIEDKVSE
                     EAGILEVNAEFSYRDFTLDDRAGSEIEFRYDHEALRPQYSFRVDDGRSTLRLDHQEYR
                     WLKIFKKDELPLWKLNLPGHIPLDLYLRAEKGYCYLRMADLDVHSLDLDCERCYDVVL
                     QFGKKIPSEPVLLDLNKSKLRVEMAEAYNVVIRDGSLLPFYLTEDLGYFEMGDDLVAD
                     TLIPPDSALVLDIAPGLRELIINRR"
     gene            2368..>3870
                     /locus_tag="ENO07_03450"
     CDS             2368..>3870
                     /locus_tag="ENO07_03450"
                     /inference="COORDINATES: protein motif:HMM:PF00082.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01053.1"
                     /translation="MKSKIIIISLSLILMFALSGILLTGQDYRPGGDQQKYWVFFRDK
                     GELSEQELDARLDALQKRATVPNLRKRAVVQAQQRLFDYSDLNVHEPYLDSLKSLGVE
                     IHRTTKWLNAASVSADQALIDNLPSLDFVDSVRPVMTYTRDIPEYHGPGEVLPKIEQT
                     FDLDYGQSFEQLQQIGVPAVHNIGFSGEGVLIGVFDTGFDPSHPAFLDMDIEATYDFI
                     NDDTDVRDDYDLQRSHGTSVLSVIGGYAPGELIGPAYNATYCLAKTEIVNLEIEIEED
                     NFIAALEWADSIGCRIVSSSLGYILWYTKDDLDGNTALITIASDLAAQKGMNVIVAAG
                     NEGPEATTILTPADGDYVLAIGGVLINDEIWYSSSRGPTADGRIKPDVCANSSNVYVA
                     SYVGGYRRSSGTSYAAPLAAGAAALLIESDPTLTSFDVIEGMRSSARQVGGVSYPNNA
                     YGYGIVDVQTAIGFEPPEFETAILAFPNPFQNNIQVNVKIEGAGRTRVSIH"
BASE COUNT          933 a          894 c         1026 g         1017 t
ORIGIN      
        1 aatgataaca gggaaaatat tgataaatat tatgaagaac gcatccgtat ggtggagaac
       61 cagatcaaac gccgcggtat tgaagacagc gcggttctgg aggccatgcg ttcggtgccg
      121 cgtcaccttt ttgtgcccga agcctaccgc ccccgtgcat acgaggacgg tcctcttccg
      181 atcggtaata accagacaat ctcacagccc tatattgtag ctttaatgac agagcttctc
      241 gatcttcagc cgggagagag ggttcttgaa gttggcaccg gttccggcta tcaggccgct
      301 gtgctggccg agatcgccga ttcggtttat acgatcgaaa ttgtcaaacc actcgccgac
      361 agggccggag agattctcga ttcaatcggt tacaataata taaaagtgat gcatggtgat
      421 ggttatcagg gctacgaaga agctgcgccc tatgatgcta tcatcgtaac tgctgccccc
      481 gatcatgtcc cccagcctct tatcgatcag ctcgccatgg gcgggcgtct ggtgattccg
      541 gtgggaagta tctaccagta cctgacgctg tatgaaaaga ccgaggacgg tcttaaaaag
      601 aaaaccggta tagccgtccg tttcgtgccc atgaccggaa aagctcagga aaaataatat
      661 ctgcagatat ccttcctgtt tcttaactgt tttagttgac tctgtttttg tgtgtgttaa
      721 tttaaaaccg actgctttga taatatgcta tccattgatt atgccgaaat ggaattcggc
      781 atcatggtta ttgagtactg ccggataggg atgtccggca gtacttttat cttgactctt
      841 gcgccacaat gatgtttctg taacaaacgg gaatcccctc cgtatagcat gttaatgaac
      901 gaggaggagt aatgaagaaa ctttacaggt caagggaatc gaatatcata gcaggggtct
      961 gcggcggttt cggtgagtat ttcaatatcg atccggtaat cgtgcgcatc atattcctgc
     1021 ttctgatccc ggccgggggt accggaataa tcgcctatat catagcctgg ataatagtgc
     1081 ctctgcgtcc tgtcaacgag gttgcgcaac cgccctcaaa ggagcagagc gaggcgatga
     1141 tgaaatacct tccgggactg gcgctgataa ttattggggc cgcctttctg atcacgcgaa
     1201 tatggggatg gctgtcgttc gggcatatct ggccggccgc attgatcgta atcggggcat
     1261 ttctaattta ccgagccgta ggctcaaaaa aggagcagta agatgcatgc gggtagcctt
     1321 aactggggtc tgattttcat tatcgttggc ctggtcgcac tgggatggac cacgggccgt
     1381 ctgccggccg aggtcttcat gcgcttgtgg gacctgtggc cggtgctcct gatcgcgata
     1441 gggatccaga tgatcttttc caagaccaaa ctggccggat ttgcttatct ttcgtcgata
     1501 ctgattattc tggctgcggc ctacgctgtt tctccatact ggcagcaggt tacgacaggg
     1561 gaaagcctgg agcgcgagtc cgggacaatc gaggataaag tcagcgagga agccggaata
     1621 ctggaggtga atgctgaatt ctcatacagg gattttacgc tggatgaccg ggccggctct
     1681 gaaatagaat tcaggtatga ccatgaagcc ctgagacctc aatattcgtt tagggttgat
     1741 gatggcaggt caacactccg gctggaccac caggaataca ggtggctcaa gatcttcaag
     1801 aaagacgaac ttcccctgtg gaaactcaat cttcccggcc atatccctct cgatctttac
     1861 ctgagagcgg agaagggcta ctgctatctc aggatggctg atttagacgt ccattctctc
     1921 gatctggatt gcgaacgttg ttatgatgtg gtgttgcagt ttggtaagaa aattccctcc
     1981 gagccggttt tgctcgatct gaataaatcc aagctcagag tggaaatggc ggaggcatat
     2041 aacgtggtta ttcgcgatgg ctcccttctg ccgttttatc tgaccgagga tctggggtat
     2101 tttgagatgg gagatgatct ggttgcggat accttaattc ccccggacag cgccctggtt
     2161 ctcgatattg cacccggcct cagggaattg attatcaaca ggagatagag gcggttatat
     2221 ttttgctgat cttttttgaa tccgcaacgg cggattgctg ttataatatg caaaaatgaa
     2281 cacttatcgg attatacagt ttctatggat gcgtcctcac gaaccgcagc tctaattagc
     2341 atacccattt agccgataga ggaatatatg aagagcaaga ttatcatcat cagcctttcc
     2401 ctgattctta tgtttgcatt gtccggcatt ttgcttaccg ggcaggatta caggccgggg
     2461 ggagatcagc agaaatactg ggtctttttc agagataagg gagagctgtc agagcaagaa
     2521 ctggatgcca gactggatgc gttacagaaa cgtgcgactg tcccgaatct gcgcaagcgt
     2581 gctgtggtcc aggcgcagca gcgcctcttt gattattctg acctcaatgt tcatgagcca
     2641 tatctcgaca gcttgaaatc tttgggtgtt gaaattcacc gcacgactaa atggctgaat
     2701 gccgcatcgg taagcgctga tcaggcatta atagacaatc tcccatcgtt agactttgtt
     2761 gattcagtca gacctgtaat gacctacaca cgcgatattc ccgaatatca cggtcccggt
     2821 gaggtattgc ccaagatcga acagaccttt gatctggatt acggacagtc tttcgagcag
     2881 ctccagcaga taggcgtacc ggctgtgcat aacattggat tcagcgggga gggagtgctg
     2941 attggtgtct ttgacaccgg ttttgatcca tcccatccag ccttccttga tatggatatc
     3001 gaggccacct atgatttcat caatgacgac actgatgtaa gggatgatta cgatctccag
     3061 cgctcgcatg gcacttcagt attgtccgtc attggcggct atgcaccggg cgaattaatt
     3121 ggaccggctt ataatgctac atactgcctg gcaaagactg agatcgtgaa tctggaaata
     3181 gagatcgagg aagacaattt tatagctgct cttgaatggg ctgattctat tggctgccgc
     3241 atagtctcct catctttggg atatattctg tggtatacca aggatgacct ggacggcaat
     3301 actgcactta ttaccatcgc ctccgatctg gctgcgcaga agggtatgaa tgttatagtg
     3361 gcggccggta atgagggtcc ggaagctact acaatattga cccccgctga tggcgactat
     3421 gttttggcga tcggcggagt tttgattaat gacgagatct ggtattcatc ctcgcgcggt
     3481 ccaactgccg atggacggat taagcctgat gtgtgcgcca atagctcgaa tgtctatgtg
     3541 gccagttatg tgggcggcta taggcgaagc agcggaacat catatgccgc gccattagcg
     3601 gccggagcgg cagccctttt gatcgagagt gatccaactc tgacctcatt cgacgttatc
     3661 gagggtatgc gctccagtgc gcggcaggtt ggaggtgtct catatcccaa taatgcttat
     3721 ggttatggta tagtcgatgt tcagacagcg atcggttttg agccgcctga gtttgagacc
     3781 gccattctcg ccttccccaa cccgttccaa aataatattc aggtgaatgt caagatagaa
     3841 ggcgccggaa gaacccgggt cagcatacat
//
LOCUS       DSEB01000189            6667 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000700, whole genome shotgun sequence.
ACCESSION   DSEB01000189 DSEB01000000
VERSION     DSEB01000189.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6667)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6667)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6667
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000700"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            39..1298
                     /locus_tag="ENO07_03455"
     CDS             39..1298
                     /locus_tag="ENO07_03455"
                     /inference="COORDINATES: protein motif:HMM:PF13439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDS01054.1"
                     /translation="MEQDSLRILMLIDQFFPILGGAEQQALRISKELTRRGFKVSVLT
                     RKSRTELAETEIYEDIRIHRLPFVGAGSAPKLKSTSPAAAWLVKNRAYYDIIHCHGSN
                     PLEWSAMVAGRITKKPYVLKLTNPNFMNYAGANNGFKMKSSGANSRPNEPVRTLMLPA
                     LRFMRRNMVKHASRVLAISPQIAGCLARSGFNNVSYIPNGIDTEFFRPVAPVDKSGLR
                     SKLGLDSGATTFLYAGRLAVEKNLMTLLKAWNQFTASNPGQKAQLLFLGDSNGQVYSA
                     EDELREYARENNLSSLKFTGSVLNVDEYLQASDIFVLPSYWEGMSNALLEAMACGLPS
                     IVSDIPANSALVANGSSGLTFPAEDVEALVKCLTALAANPDIRVDMGDKAREIAVEKF
                     SFPAIIDGISEIYGAILGYSVSSQRVL"
     gene            1295..2653
                     /locus_tag="ENO07_03460"
     CDS             1295..2653
                     /locus_tag="ENO07_03460"
                     /inference="COORDINATES: protein motif:HMM:PF04932.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01055.1"
                     /translation="MNLLTTIPAGLILILAAGLSINRPLWLLGGYLVLVPLFPPLPLG
                     QIELSMLDLLAVPAAIRIICNLSRSGFEIKGGIVKGMFILVAAAIFSYISFCFQTQIL
                     SAALLFRIIRLIEMFLPVILAYQTFRDYPTHRIIKLFLIGAGLTAAVAVFMFINGISL
                     RASQTFIEDGLEFFRAGGTHGDSGSLGSFMGVSVLVSIWTLLYYNRHGLAKWALISGG
                     LSGLTLLMTLSRGGLILAASGVLILMMPLLRNPGKLIRAAILSVLFVTAGSIAASKFM
                     NHDLITMAASEFGQRVTGLSEISSDFETVSSHRVVHWKQGLNLYKASALAWPFGLGYK
                     SLKLHYDTLPDNNFNQALFEMGVFGLLALLVMIGSGIKEGLLQYRFNRDFGLLGIALW
                     TGLISNMFSADVLTYWHNIPAIFILLIAIGKNAGINVDYNQYNHRYYAEYEAYNSQAN
                     VR"
     gene            2681..3904
                     /locus_tag="ENO07_03465"
     CDS             2681..3904
                     /locus_tag="ENO07_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDS01056.1"
                     /translation="MNIKTLKTAVIHDWLVTYCGAERALEEIINLFPECDLYSLIDFL
                     PENHRDFIHRKKVKTSFLQRMPLVKSRYRSYLPLMPLAVEQFDLSGYDLIISNSYAVA
                     KGVITGPDQLHISYQHSPIRYAWDMTHQYLADAGLSRGVKSCIARTILHYIRNWDARS
                     SNGVDYFIANSGFISRRIMKCYRRRSTVIYPPVDVFSFMVDDNKEDFYLTVSRLVSYK
                     KIGLIVEAFASMPDKRLIVIGDGPEFKEIRQKASSNIEMMGYQPRAIVQKYMQKAKAF
                     IFAAMEDFGIVPIEAQACGTPVITFRKGGALETVIENQTGLFFEEQTAQSIARAVRRF
                     EEMPAFCQNTMRRNALRFSKERFRQNFMEFVEDKIERFFGQTTHSRDNIDRIAEIIAQ
                     DENQIEEKNFQIANK"
     gene            4083..5165
                     /locus_tag="ENO07_03470"
     CDS             4083..5165
                     /locus_tag="ENO07_03470"
                     /inference="COORDINATES: protein motif:HMM:PF02706.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01057.1"
                     /translation="MSESDNMKKDMPWLEERHQGREFLRINYFELAALFLKNKKWIFG
                     TALSVMILAAVILFLTPNKYSSHARILPSGKADRLSALQELAQMNGLTSMDENSSELF
                     PVILKSRHICTAVLQKEYPYRNTNITLEEYFDTNDPDRLLAALAEITSVSMDKKSGVI
                     DVSVETRSAELSQAVLQAYITELENYNIHKRRSQAKEASKYLEKQLVEIEKELKQAED
                     KLESFQNANRGWASSSNPEILKMLARYQRDIEIKTRTYLTLRQEYELAKLNARKDIPV
                     VRVLDQPSLPTVKSGPKRLSAIILAGFAAFVTAYIVVIILNSMRRAGNGPDRESFQEL
                     REDLKKEFPRVIQLIEKTRRRQRIET"
     gene            complement(5166..5993)
                     /locus_tag="ENO07_03475"
     CDS             complement(5166..5993)
                     /locus_tag="ENO07_03475"
                     /inference="COORDINATES: protein motif:HMM:PF13489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDS01058.1"
                     /translation="MDQNDDSLYGASEADFFRKRTKSWDKRDLEKFDLMLKYCEGCNR
                     LVDIGCGWGKFLEIASSHVDEVWGVDESVEMTKGVEQNCPSAKVVICRADNLDLPSDY
                     FDIAVTSQLLHEIKLFGMLDELYKSLSEIKRVLAVGGKHLLVDHLDAGDGNVDVFLPP
                     DKANLLQEFESKFEYYQAKHSVMAGDIIRIPRRTLQDFLTKDAYLNSPMESMEMNETH
                     NVFTREETEQYLDAVGLAMQEWTEFTDISHDLMKKGGRFISGKPWMRKFLCVAVKTE"
     gene            complement(5980..>6667)
                     /locus_tag="ENO07_03480"
     CDS             complement(5980..>6667)
                     /locus_tag="ENO07_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015116945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDS01059.1"
                     /translation="RIIWLENGGIYAQASLRGGREYGEEWHRAGMLENKQNVFDDFIA
                     CAEWLIENNYTNSSRLAIEGGSNGGLLVAACMVQRPDLFGAVLCRVPVIDMLRYHMFT
                     VGHFWTGEYGNAEENPEHFDFMYAYSPLHNIEKGETYPATLITTADADDRVVPMHGKK
                     FAAALQYADSGENPILLRVDTKAGHGHGKPTSKRIEEYADIYAFLFEALNIEPVYLEE
                     AAEGLSNGPK"
BASE COUNT         1737 a         1620 c         1585 g         1725 t
ORIGIN      
        1 gccgtctcaa gcgtctgaaa acttcggagg agcgataaat ggaacaggat tcgctgagaa
       61 tattaatgct gatcgatcaa ttttttccaa ttttaggagg agccgagcaa caggctttgc
      121 gaataagcaa agaattgacc aggcgaggtt ttaaagtcag tgtgctcacc cgtaagagca
      181 ggactgagct ggctgaaaca gaaatatatg aggatattag aatccacagg cttccattcg
      241 tgggagcagg cagtgcacct aaactcaaga gtacatcccc ggctgccgcc tggctggtaa
      301 aaaacagagc ttattatgac atcatccatt gccatggttc aaatcctctg gaatggtcgg
      361 ctatggttgc ggggcgtatt acaaaaaaac cgtacgtctt gaaactgacc aatcctaatt
      421 ttatgaatta tgccggggcc aataatggat ttaagatgaa atcatccggc gcaaatagcc
      481 ggccaaatga gcctgtcaga acgctgatgc ttccggcctt gagattcatg cgcagaaaca
      541 tggtaaaaca tgccagcagg gtcctggcga tcagccccca gattgcaggc tgccttgccc
      601 ggtccgggtt caataatgtc tcatatattc ccaatggcat cgacaccgaa tttttcagac
      661 cggtcgctcc cgttgataaa tcgggcctga gaagcaagct gggactcgat agcggggcaa
      721 ctacattcct ttatgcggga cgtcttgctg ttgaaaagaa tctcatgact ctgttaaagg
      781 catggaacca gtttactgca tcgaatccgg gccagaaagc gcagctgctt ttcctgggcg
      841 actccaacgg gcaggtatac tcggctgaag acgaactgag agaatatgct cgagaaaata
      901 atttaagcag cctgaaattt accgggtcgg ttttaaacgt cgacgaatat ttgcaggcct
      961 cggatatctt tgtgttgccg tcatactggg aaggtatgtc taatgcgctg ctcgaggcaa
     1021 tggcctgcgg gctaccctcc atcgtctccg atattccggc caactctgcc ctggtagcaa
     1081 atggatcatc cggattgaca tttcccgcag aagatgtaga agcgttggtt aaatgtctaa
     1141 cagcgctggc cgccaaccct gatatcagag tcgatatggg agataaagcg cgggaaatcg
     1201 ctgtggagaa gttttctttt cccgcaatca ttgatggcat atccgaaatt tacggcgcaa
     1261 tactgggata ttccgtctca tcgcaaagag tgttatgaac cttttaacta caatcccggc
     1321 aggtctgatt ttaatcctgg cggccggtct ttcgatcaac cggcccctct ggcttctggg
     1381 cggctatctg gtactggtgc cattattccc gccattgcca ctcggtcaga tcgaactttc
     1441 catgctcgat cttctggctg ttccggcggc catcagaatt atctgcaacc tctcccgttc
     1501 cggatttgaa attaagggcg ggattgtcaa gggtatgttt attctggtgg cggcagcaat
     1561 attttcctat atcagcttct gcttccagac acaaatcctg tcagcggctt tgttattcag
     1621 gatcattaga ttgattgaga tgtttctgcc tgtaatcctg gcgtatcaga cattccgcga
     1681 ttacccgacc cacaggatta tcaaattgtt cttgataggc gcgggtttga ccgctgctgt
     1741 ggcggtgttt atgttcatta atggtatatc actcagggcg tcgcagactt ttattgagga
     1801 tggcctggaa tttttccgcg ccggcggtac acacggtgac tcgggctcat tgggaagctt
     1861 tatgggtgtg agtgtgctgg tgtcgatatg gacgctgctc tattacaacc gtcacggtct
     1921 tgcgaaatgg gctttgattt caggcggact ttccgggctg acgcttctca tgacgctttc
     1981 acggggcggt ctcatccttg ccgcatctgg tgtattgata ctgatgatgc cgttattgag
     2041 gaatccggga aaattaataa gggcggccat tctgtctgtg ctgtttgtga ctgcagggtc
     2101 aattgccgct tcaaaattta tgaaccatga cctgataaca atggccgcct cggagtttgg
     2161 gcaaagagtg accggccttt cggaaatatc cagcgacttc gaaactgtat ccagccaccg
     2221 ggttgtccat tggaagcagg gactgaatct gtacaaagct tcggcattgg cctggccgtt
     2281 cggattggga tataaatcat tgaaactgca ctacgacact ctcccggata acaacttcaa
     2341 ccaggcgctc ttcgaaatgg gtgtttttgg tcttttggcg ctcctggtca tgataggctc
     2401 aggcattaag gagggattac tgcagtacag attcaacagg gatttcggcc ttcttggtat
     2461 cgcactttgg acggggctta tttcgaatat gttctcggcc gatgttttaa cttactggca
     2521 caatatcccg gccatattca tcctgcttat agccatcgga aaaaatgcag gcattaatgt
     2581 agattacaat caatataacc ataggtatta tgcagaatat gaggcttata attcacaggc
     2641 aaatgtccga tagaattaca gggtagttgc tggaaccata atgaatataa aaacactaaa
     2701 gacagccgtc atacatgact ggctggtcac atactgcggc gctgaacgtg ccctggagga
     2761 gattataaat ctctttcctg aatgtgatct ttactccctg atcgacttcc ttcccgaaaa
     2821 tcatcgtgac ttcatacatc ggaaaaaagt caagacctct tttctgcaaa gaatgccgtt
     2881 agtaaaaagc aggtaccgtt cttaccttcc gcttatgccg ctggcagtcg agcaattcga
     2941 tctctcaggg tatgatctta ttatatccaa ttcctatgct gtcgcaaaag gtgtcataac
     3001 cggcccggat cagctgcaca tatcttatca gcattcgccc attcgttacg cctgggacat
     3061 gacgcatcag tatctcgctg atgccgggtt aagcaggggt gtgaaaagct gtatcgccag
     3121 gacaattttg cattatatcc gcaactggga tgcgcgttca tcaaacggcg tcgattattt
     3181 tattgccaat tcgggattca tctcccgcag gatcatgaaa tgctaccgcc gcaggtcaac
     3241 tgtaatctac cccccggttg atgtattctc ttttatggtt gatgataaca aggaagattt
     3301 ctacctgact gtgtcgcgcc tggtatcata caaaaaaata ggccttattg tggaggcctt
     3361 cgcatccatg cccgataaaa ggctgatcgt tatcggtgat gggcctgaat ttaaggaaat
     3421 caggcagaaa gcgtcctcga atattgaaat gatgggctac cagccccgcg ctattgttca
     3481 aaagtatatg caaaaagcca aagcgtttat ctttgcagcc atggaggatt tcggaatcgt
     3541 gccgatcgag gcccaggcct gcggaacacc tgtcatcact tttaggaagg gcggggcgtt
     3601 agagacagtg atcgagaacc agaccggcct gttcttcgag gagcagactg cccaatcgat
     3661 agctcgggcc gtgaggaggt tcgaggagat gccggctttt tgtcagaata ctatgcggag
     3721 gaatgcgctc cgtttttcaa aagagcggtt tcggcagaat ttcatggaat ttgtggagga
     3781 caagattgaa cggttcttcg gtcagaccac ccattccaga gataatattg acagaattgc
     3841 ggagatcata gcgcaagatg aaaatcaaat tgaagagaag aatttccaga ttgcaaacaa
     3901 gtaatctttt caagctgatc tggaaaaact ttccataacg attaatgaaa ctttgaggcc
     3961 caatgtcgga actaaattgc tctaacatat taataatatg cagcttagaa aataactacg
     4021 ggagtctgct cgagatggca gtctatttgc aggcaatata ggcggcttaa tacagggaaa
     4081 gtatgtccga gagcgataat atgaaaaaag atatgccctg gctcgaagaa cggcatcaag
     4141 gacgggagtt cttgcgcatc aattactttg agctggccgc tttatttttg aagaacaaga
     4201 aatggatatt cggtacagcc ctgtctgtga tgattctggc ggcagtcatc ctgtttttga
     4261 caccgaataa atacagctcg catgcacgca tacttcccag cggcaaagct gaccgccttt
     4321 ccgcacttca ggagctggcc cagatgaacg gccttaccag tatggatgag aactcgtccg
     4381 agctgtttcc ggtaatcctg aaatcaaggc atatttgcac ggcggtcctg caaaaggaat
     4441 acccatacag gaacacgaat attacccttg aagaatattt tgatacaaat gatccggacc
     4501 gccttctggc cgcgcttgct gaaatcacct ccgtctccat ggacaaaaaa agcggtgtga
     4561 ttgatgtaag tgtcgagacc agatccgccg aactctcgca ggcggttctg caggcttaca
     4621 ttacagaact tgagaattac aatatccata aacggcgctc acaggccaag gaagcctcca
     4681 agtatctcga aaaacagctg gttgaaatcg aaaaggaact caagcaggcc gaggataaac
     4741 tggagagttt ccagaatgcc aaccggggat gggcaagcag cagtaatccc gagattttaa
     4801 agatgcttgc gcgctatcag cgcgacattg aaatcaaaac caggacatat ttgaccctcc
     4861 gccaggaata cgagcttgca aagcttaatg cccgcaaaga tatcccggtg gttagagtac
     4921 ttgaccagcc ctctctgccg acagtgaaat cggggccaaa acgtctttcg gccataatcc
     4981 tggccgggtt tgcagctttt gtgacagcat atattgtggt gattattctg aattccatga
     5041 gaagagcggg aaacggtccc gacagggagt cgtttcagga attacgagag gatttaaaaa
     5101 aagaattccc gagggttatc cagcttatcg agaaaaccag aaggcgccaa aggatcgaaa
     5161 cttagctact ctgttttcac cgccacgcat aaaaatttgc gcatccaggg cttacccgaa
     5221 ataaatcttc cgcctttttt catcagatca tgcgagatat ccgtgaattc ggtccattcc
     5281 tgcattgcca gaccgaccgc atccagatac tgctcagtct cctcgcgtgt gaagacatta
     5341 tgggtctcgt tcatctccat cgattccatg ggcgagttca aataagcatc tttggtaaga
     5401 aagtcctgca atgttctcct ggggatacgg attatatcgc ccgccataac actgtgtttc
     5461 gcctggtaat attcgaattt gctctcgaat tcctgcaata aattggcttt atcaggcggt
     5521 agaaagacat ccacattccc gtcccccgca tccagatgat ccaccagaag gtgctttccg
     5581 cccaccgcca gtacccgttt aatttcgctc agacttttgt acagttcatc cagcatgccg
     5641 aatagcttta tttcatgaag caactgcgag gtcacagcga tatcgaaata gtctgaggga
     5701 agatcaaggt tgtccgcccg gcaaattacc actttcgcgc tgggacaatt ctgctcaaca
     5761 cctttagtca tctcaacgct ttcatcaact ccccaaacct catcaacatg actcgaggca
     5821 atctccagaa atttacccca tccgcagccg atatccacga gccgattgca gccctcacag
     5881 tatttaagca tcaaatcaaa tttctcgaga tcccgcttat cccaggattt cgtgcgcttc
     5941 ctgaaaaagt cggcctctga tgctccgtaa aggctatcgt cattttggtc cattagaaag
     6001 cccctctgct gcttcttcca gatatacagg ctcaatattc agtgcctcaa ataaaaaggc
     6061 ataaatatcg gcatattctt cgattctctt tgaggtcggt ttgccatgcc catgaccagc
     6121 cttcgtatcc acccgcagga gtatcggatt ttctccgctg tcggcatact gaagggccgc
     6181 cgcgaatttc ttgccatgca tcggaaccac acgatcatcg gcatcagctg tcgttataag
     6241 ggttgccgga taggtctcgc ccttttcaat attatgtaag ggtgaatatg cgtacataaa
     6301 gtcgaaatgc tcgggatttt cctcggcatt gccgtattcg ccggtccaga aatgcccgac
     6361 tgtgaacata tgataacgca gcatatcgat tacgggcaca cggcacagca cagcaccgaa
     6421 taaatccggc cgctgtacca tacaggccgc caccaaaaga ccgccgttgc ttccgccctc
     6481 gattgccaga cgagagctgt tggtatagtt gttctcgatc agccactccg cgcatgcgat
     6541 aaaatcatcg aaaacattct gcttattctc gagcattccc gcccgatgcc attcctcgcc
     6601 gtattctctc ccaccgcgca gtgaggcctg cgcatatatt ccgccgtttt ccagccagat
     6661 aatacgc
//
LOCUS       DSEB01000190            3669 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002634, whole genome shotgun sequence.
ACCESSION   DSEB01000190 DSEB01000000
VERSION     DSEB01000190.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002634"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(232..2736)
                     /locus_tag="ENO07_03485"
     CDS             complement(232..2736)
                     /locus_tag="ENO07_03485"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00132.22,HMM:PF02878.14,HMM:PF02880.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDS01060.1"
                     /translation="MKAIIMAGGFGTRLRPLTLGVPKPMVPVANKPMMEHVVNLLKKY
                     GFDEILSLLYFQPEAITDYFGDGSDFGVKMRYKLAEDDFGTAGSVRNAYEDFFEDERI
                     LIISGDVLTDFDLSSVISFHEDRKADATMVLTQMENPLSYGVVITDHDGKITRFLEKP
                     TWGEVFSDQINTGIYVLEPQVTRQIPNKEFYDFSKDLFPKMLSEKQALYGCNIQGYWR
                     DIGNVGEYTSAHQDILRGEVIIDLGYNMLKHDGSILWIGKNQNVDDSVKYEGVVILGD
                     KVSIEPDCKIMNSTIGDNCIIGAKSRISNSIIWNDSVISEQTEITQALLMNETRVGAR
                     AKIYENAIISEGVTLGDEVSISPNVKIWPKKEVENGAVLSTSLVWGEKWNRELFTDAK
                     VTGLGNLEITPEFAAKLGAAYGAMLGAGSSVVYSRDVSLSSRMIQRAFGAGLISAGIN
                     VRDLQTLPIPVVRFELYTGRHAGGVHIRHTPYRKELQDIIFFSEDGQDIATSKAKSLE
                     RLFVREDFRRARIDEVGTIEYPVRILESYREQFLKYIDVEAIRKANFKIVVDYAHGGA
                     VDIFPAIFGELGCEVISLNAYIDPSKVSISREEKNFAMKTLSVIVKSLGANLGIMLDN
                     NAEKIRIVDEEGTEIAEQLLLLLVTDLFLSVTRARKIAVPVLASMGVEEIAAEYGVEV
                     IRVRNDHQAMMHAKTGLDVDFVGGTRGGFIFPGFQLGADAMFSTAKILELLARTDQRF
                     GGLREKFSDLIYLSEDVPCNWRKKGQVMRNLITHTADEDRQLVDGIRVLRDGGWVLIA
                     PDREKDLFHIYSESRDKDMAHKYINEFRQKIDAMQK"
     gene            complement(2741..3415)
                     /locus_tag="ENO07_03490"
     CDS             complement(2741..3415)
                     /locus_tag="ENO07_03490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01061.1"
                     /translation="MNYSLFIDFDYTITTDDVGNRFYTYFSQGKNEPLVKRWLNREIT
                     THECLTKEAGLCRGTREEFIEYIERFEIDTGFPELVRLCAEANIPLYVLSDGLDFYIR
                     HILGKYGINEINIYSNIAVFNNGFLEIELPHWTEECASCGNCKGERIRQLRRKDDRVI
                     YVGDGLSDLCGAREADLIFAKDDLAKFLEQEDREYIEYENLFEVRDKLIGILNSARNK
                     GAASRG"
BASE COUNT          926 a          990 c          787 g          966 t
ORIGIN      
        1 atattaaact tcatgtaacg gcacttttct taattattaa tcagcaatga tatactcaca
       61 tctgttcttt gtcaagttaa ttaaagtcaa cagattgcga atagggcatt gactatttat
      121 gataaaaccc cgtttccgga aagttccatc agataagaaa agccgaggac taaagcaaca
      181 ccccaattat tactcagaca tttatccttg atgcgattta cctgcgtgag actatttctg
      241 catggcgtca atcttttggc ggaattcatt gatatattta tgggccatgt ccttgtctcg
      301 cgattcggag tagatatgaa agagatcttt ctccctgtcc ggcgcaatca gcacccagcc
      361 cccatcccgc aaaaccctga taccgtcgac aagttgacga tcctcatcgg cagtgtgagt
      421 aatcaaattc cgcattacct ggcccttctt gcgccagtta cagggcacat cctcactcag
      481 gtagatgaga tcagagaact tctcgcgaag ccctccgaac ctctgatccg tacgggccag
      541 aagctccagt atcttggccg tggaaaacat cgcatccgca cccagctgga agcccggaaa
      601 tataaacccg ccccgggtgc ctcccacaaa atcaacatcc aaaccggttt ttgcatgcat
      661 catagcctgg tgatcgtttc tcacccggat aacctccacc ccatattctg cggcgatctc
      721 ctccacgccc atggaggcca gcaccggcac agcgatcttt ctggcccgag tgactgaaag
      781 aaagagatcg gtcacgagca atagaagcaa ttgttccgct atttccgtcc cttcttcgtc
      841 cacaatcctg atcttttcgg cattattgtc cagcataatc cccaggttgg cccccagtga
      901 tttgacgatc accgacaggg tcttcatggc gaagttcttc tcctcgcggg agatggagac
      961 cttggatgga tcgatgtagg cattgagaga aatgacctcg cagcccagct ccccaaaaat
     1021 ggccggaaat atatccaccg cgccgccatg ggcatagtca accacgattt tgaagttcgc
     1081 tttccgtata gcctcgacat caatgtattt gaggaattgt tccctgtagg attcgagtat
     1141 gcgcacagga tattctatgg tcccgacctc gtcgatcctg gcgcgcctga aatcctcgcg
     1201 cacgaacagt ctttccagtg atttcgcttt cgaggtggcg atatcctggc catcctcgga
     1261 aaagaagata atatcctgca attccttgcg gtaaggcgtg tggcggatat gtacccctcc
     1321 tgcgtgtctg ccggtgtaaa gctcgaacct gaccaccgga atcgggagag tctggagatc
     1381 cctgacattg atcccggccg atatcaatcc ggctccaaaa gcgcgctgaa tcatcctgga
     1441 agaaagcgag acatcccgcg aataaaccac cgagctcccg gcccccagca tggcgccgta
     1501 ggccgctccc agtttggcgg cgaattccgg ggtaatctcg aggttgccca gaccggtaac
     1561 tttggcgtcg gtgaaaagct cgcgattcca tttttctccc cacacaagac tggttgacag
     1621 aaccgcgccg ttttccactt cctttttggg ccagattttt acattgggag aaatcgaaac
     1681 ctcatcgcca agagtgacac cctcggaaat tatcgcattt tcatagattt ttgcacgggc
     1741 cccgacacgg gtctcgttca ttaaaagcgc ctgggtgatt tcggtctgct cgctgatgac
     1801 cgagtcattc catatgattg aattcgaaat cctggatttg gctcctatga tgcagttgtc
     1861 acctattgta gaattcatga ttttgcagtc cggctcaata ctgaccttgt cccccagtat
     1921 aaccacgccc tcgtatttca ccgaatcatc gacattctgg ttcttgccga tccagagtat
     1981 cgaaccgtca tgcttgagca tattataacc cagatcgata ataacctcgc ccctgagtat
     2041 atcttgatga gcgctggtat attcacccac attgccgata tcgcgccagt aaccctgaat
     2101 attgcagccg tagagggctt gtttttcact cagcatttta ggaaataagt ctttagagaa
     2161 atcgtaaaat tctttattgg gaatctgcct cgtcacctgt ggttcaagca catagattcc
     2221 ggtatttatc tggtctgaga agacctcgcc ccaggtcggc ttttcgagaa aacgagtgat
     2281 cttgccatca tgatctgtta tgaccacccc gtaagagagc ggattctcca tctgggtcaa
     2341 taccatggta gcatcagctt tacgatcctc atggaaggaa atgactgaag agaggtcgaa
     2401 atccgtcagc acatcccctg atatgatcaa aatcctttca tcttcaaaga aatcctcata
     2461 ggcattacgc accgatccgg cagtaccgaa atcgtcctcg gccagcttgt aacgcatctt
     2521 gaccccgaaa tccgagccat ctccaaaata atccgtgatt gcctccggct ggaaatagag
     2581 aagcgataaa atttcatcaa agccgtattt cttgagcagg tttaccacat gctccatcat
     2641 cggtttgttg gccaccggaa ccatcggctt gggtacccca agcgttaatg gacgcagtcg
     2701 tgttccgaac ccccctgcca ttattatcgc tttcatagta ttaccctctc gaggctgctc
     2761 ccttattgcg ggcagaattt aagatcccta ttaacttatc cctgacttca aacagattct
     2821 catattcgat atattccctg tcctcctgct ccaggaattt tgccagatca tccttggcaa
     2881 atatcagatc ggcctccctg gctccgcata aatccgacag gccatcgccg acatagatta
     2941 ccctgtcatc tttgcgcctg agctgacgta ttctctcccc tttgcagttc ccgcaggagg
     3001 cgcattcctc ggtccagtgc ggcagttcga tttccagaaa gccgttatta aagaccgcta
     3061 tatttgaata tatgttgatt tcattgatgc cgtattttcc gaggatatgc cggatataaa
     3121 aatccagccc atcgctcaaa acatacaggg gtatatttgc ttcggcacag agcctgacaa
     3181 gctcgggaaa accggtatcg atttcgaacc gctcgatata ttcgataaac tcttcccgtg
     3241 tgccgcggca taggccggcc tcctttgtca ggcattcatg cgtggttatt tcccggttca
     3301 gccagcgttt taccagaggc tcattcttgc cctgagagaa ataagtataa aatctattgc
     3361 ccacgtcatc ggtggtgatt gtgtagtcga aatctatgaa aagcgagtaa ttcataacaa
     3421 aattcgaatc ccgtgtgatt ttacgctaat ataaaaagaa gacaggtaac aaaaaagcat
     3481 aaactacatc aaatctgcgc atttggtaaa caaggtgtga tctttcttca ctccatgaga
     3541 caaaatcagc cttccggctc cagaatttgc ctgtattccg cgcgattctc caaaaggctt
     3601 atagccgcgc ggacaaccgg atcataacga agatagtatt tctcgtagat ttctgtctca
     3661 ccataggcc
//
LOCUS       DSEB01000191            5264 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001168, whole genome shotgun sequence.
ACCESSION   DSEB01000191 DSEB01000000
VERSION     DSEB01000191.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5264)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001168"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..172)
                     /locus_tag="ENO07_03495"
     CDS             complement(<1..172)
                     /locus_tag="ENO07_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDS01062.1"
                     /translation="MSSRLDLKQAKYNRRVKRVRAKVVGTPERPRLSVSKSLKHVYAQ
                     VIDDIAGETLAFA"
     gene            complement(182..718)
                     /locus_tag="ENO07_03500"
     CDS             complement(182..718)
                     /locus_tag="ENO07_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDS01063.1"
                     /translation="MSRIGKQPIHIPDKVKVEIKNSTVVVKGPRGELTQEIHPRIQVD
                     IEDNVLNVKRPSDSKFFRAQHGLVRALINNMVIGVTEGFSKVLEIVGVGYRAEMANKL
                     LKLSLGFSHPIYFAPPPGIDIGLESQQKIKISGSDKALVGLVAAKIRSFRPPEPYKGK
                     GIKYDNEQVRRKAGKTAA"
     gene            complement(728..1126)
                     /locus_tag="ENO07_03505"
     CDS             complement(728..1126)
                     /locus_tag="ENO07_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007791424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDS01064.1"
                     /translation="MSMTDPVSDLLVRIKNAAKAKHKAVDVPAAKLKNEIARLLSELR
                     FIKRSSVIQIDGKPVLRIWLRYNKNDESIISGLERISKPGLRVYASSDDLFKLQQEVG
                     TMIISTSRGVMTDQQALKANIGGELVCRVW"
     gene            complement(1140..1325)
                     /locus_tag="ENO07_03510"
     CDS             complement(1140..1325)
                     /locus_tag="ENO07_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDS01065.1"
                     /translation="MAKKCLIAKAKRKPKFSVRSYNRCRRCGRPRAYMRKFGICRICF
                     RELALNGEIPGVVKASW"
     gene            complement(1335..1874)
                     /locus_tag="ENO07_03515"
     CDS             complement(1335..1874)
                     /locus_tag="ENO07_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDS01066.1"
                     /translation="MARLKEYYYKEVLPKLQKEFDYKNVMEVPRLKKIVMNIGVGEAI
                     TNPKFLDAAVEDLRIISGQQPIVTRAKKSISNFKLRAGMPIGCAVTLRRERMYEFFDR
                     LINVAIPRIRDFRGLPPKGFDGRGNYTLGLREQLIFPEIDYDKIDKVRGLSVTFVTSS
                     NDDEGAFALLRELGMPFRK"
     gene            complement(1874..2197)
                     /locus_tag="ENO07_03520"
     CDS             complement(1874..2197)
                     /locus_tag="ENO07_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000558200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDS01067.1"
                     /translation="MRIKKGDQVKVISGKNKGKIGKVLRVIPETNRVLVEGVNLIYRH
                     SRPSQRNPKGGIIEKEAPINASNVMLFDTRSNKPTRVNYKVLEDPEKKTKNKVRISNK
                     SGEII"
     gene            complement(2205..2573)
                     /locus_tag="ENO07_03525"
     CDS             complement(2205..2573)
                     /locus_tag="ENO07_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDS01068.1"
                     /translation="MIQEYSRLNVADNTGAKKVMCFRVLGGTKRRYARVGDIIVCAVK
                     DAIPNGTVKKSDIVKAVVVRTAFPVRRKDGSYIKFGDNAAVIINDQMEPRGTRIFGPV
                     ARELREKKFMKIISLAPEVL"
     gene            complement(2575..3000)
                     /locus_tag="ENO07_03530"
                     /pseudo
     CDS             complement(2575..3000)
                     /locus_tag="ENO07_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388100.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
     gene            complement(3098..3520)
                     /locus_tag="ENO07_03535"
     CDS             complement(3098..3520)
                     /locus_tag="ENO07_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDS01069.1"
                     /translation="MLMPKRVKYRKQQRGRMRGKAYRGSKISFGEFGLKAMAPAWITN
                     RQIEAARVALTRKIKRGGKVWIRVFPDKPFTKKPAETRMGKGKGAPEYWVAVVKPGRI
                     LFELEGVSEELARQAMKLAADKLPIKCRFVTRQEIGGA"
     gene            complement(3616..4503)
                     /locus_tag="ENO07_03540"
     CDS             complement(3616..4503)
                     /locus_tag="ENO07_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018475623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDS01070.1"
                     /translation="MGQKTNPVGLRLGIIRSWNSKWFAERNFADLLEEDLKIRRYISK
                     RLENAGLAKVEILRAPKKITVDIHSSRPGIVIGRKGAEVDKLREELQLITRKDITLNI
                     IEVKKPELNAKLVADSIARQLEGRVSFRRSMKKAITASMKMGAEGIKITCGGRLGGAE
                     IARSEKYMEGRVPLHTLRADIDYAISTANTTYGTIGVKVWICRGEILDKPGMEDAGES
                     RQPEMDQRRARPSGPRRRKPRPKSRVRKKRPEDEGGRRAEGQQQQRNQGRPEPRASTE
                     AKRTASRPESGDKSEDKKG"
     gene            complement(4518..4949)
                     /locus_tag="ENO07_03545"
     CDS             complement(4518..4949)
                     /locus_tag="ENO07_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDS01071.1"
                     /translation="MESVARSKYLRMSPRKMRRVAELVKGKMVHEAVDLLRYVPKRAA
                     VPLSKTIKSAAANMIAQEGTARVKAEELRVTSIIIDGGPILKRFRAVGMGRAFRIRKR
                     TCHLSVRVSDEGRAERRLAREHARRTRKRKPTKPSVAEEEE"
     gene            complement(4960..5247)
                     /locus_tag="ENO07_03550"
     CDS             complement(4960..5247)
                     /locus_tag="ENO07_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDS01072.1"
                     /translation="MARSLKKGPFIDDHLMIKIEKMNDSGDKKVIKTWSRRSTISPEF
                     VGHTIAIHTGNKFIPVYITENMVGHKLGEFAPTRIFRGHAGKLTERSSSIK"
BASE COUNT         1133 a         1517 c         1146 g         1468 t
ORIGIN      
        1 atgcgaacgc cagtgtctct cccgcgatat cgtctatcac ctgagcatag acatgcttca
       61 gggatttgct cacgctcagg cgcggacgtt cgggggtacc gacaaccttc gctcgaaccc
      121 gcttgacgcg gcggttgtat tttgcctgtt ttaaatcaag tctactcgac ataacatcat
      181 cctacgcagc agtctttcca gccttgcggc ggacctgctc attatcgtat ttaatacctt
      241 ttcctttata tggttcgggc ggcctgaacg atctaatctt ggccgcaaca agcccgacca
      301 gagctttgtc gctgccggag atcttaatct tctgctggct ctccaggccg atatcgatcc
      361 ccggaggagg cgcaaaataa atcggatgcg aaaagcccag cgagagctta agcagtttat
      421 tggccatttc ggctctatag ccgacgccca cgatttccag aactttagaa aatccctcgg
      481 tcacgccgat taccatgtta tttatcaacg cccttaccaa accatgctgg gccctgaaaa
      541 acttggaatc ggaaggtctc ttgacgttca ggacattgtc ctcaatatca acctgtatcc
      601 ggggatgaat ttcctgcgtc aattcgcccc tgggaccctt caccacgaca gtggagtttt
      661 tgatctccac cttgaccttg tccgggatat gaattggttg ttttcctatt ctcgacatat
      721 tactatccta ccaaactctg cagaccagct ctccgccgat attggcttta agcgcctgct
      781 ggtcagtcat aactcccctc gaggtcgata taatcattgt tccgacctcc tgctggagct
      841 tgaagagatc atcggatgat gcatacaccc tcaggcccgg tttggagatt ctctccaagc
      901 ccgaaattat ggattcatca tttttattat agcgcagcca aatccgcaaa accggcttac
      961 cgtcaatctg gatcaccgag gagcgcttga tgaacctgag ctcgcttaat aacctggcga
     1021 tctcattctt aagcttcgca gccggtacat ctaccgcctt atgcttggcc ttggccgcat
     1081 ttttgattct aactaagaga tcagagaccg gatcagtcat actcataatt tctaaactcc
     1141 taccaactgg ccttgaccac gccgggtatt tcgccattta aggccaattc acggaaacaa
     1201 atacggcaaa taccaaactt gcgcatatag gcacgcggcc ggccgcaacg ccgacagcga
     1261 ttatacgaac gtacgctgaa tttcggttta cgtttggctt ttgctatcaa acacttcttg
     1321 gccactctat ctccttactt tctgaaaggc atccccagtt cccgcaagag cgcaaaggct
     1381 ccttcatcgt cattggaact ggtgacaaaa gttacagata atcctcgcac cttgtcgatc
     1441 ttatcataat cgatctcagg gaaaatgagc tgctcgcgca gtcccagagt ataatttccg
     1501 cgtccgtcaa aacccttggg cggaagtccc ctgaaatccc tgattcgcgg aatcgccaca
     1561 tttatcaggc ggtcgaaaaa ttcatacatc ctctcgcgcc tcagggtcac cgcacagccg
     1621 atcggcatgc cggcgcgcag cttgaaattc gaaatcgatt ttttggcacg ggtaacaatc
     1681 ggctgctgtc cggaaatgat tctcagatcc tcaaccgccg catccaaaaa cttagggttc
     1741 gttatggcct ccccgacgcc aatgttcatg acgatctttt tgaggcgcgg aacctccata
     1801 acattcttat aatcgaactc cttctggagt ttcggtaaaa cctctttata ataatattcc
     1861 ttcaaacgtg ccatcagata atctctccgg atttattgga gatccgaacc ttattcttgg
     1921 ttttcttttc gggatcctcc agcaccttgt aattgacgcg tgtgggtttg ttgctgcgag
     1981 tatcgaacaa cataacattc gacgcgttaa tcggggcctc tttctctatt ataccgccct
     2041 tgggatttct ctggctggga cggctatgac gatagatcag gttaacgccc tccacaagga
     2101 cacggttggt ttcgggaatg acccgcaaga ccttgccgat cttgccttta ttctttccgc
     2161 taataacctt tacctgatcg ccttttttaa ttctcatcgt ttcctcacaa aacttccgga
     2221 gccagtgata ttatcttcat gaattttttc tcgcgcagct ccctggcaac cgggccaaag
     2281 atacgcgtgc cgcgcggctc catctgatca ttaataataa ccgcggcatt atcgccaaac
     2341 ttgatatagc tgccgtcctt ccggcgcacc ggaaaagccg ttctcaccac caccgctttg
     2401 acgatatcgg attttttcac cgtgccgttc gggatcgcat cttttacggc acacactata
     2461 atgtctccaa ctctggcata gcgccgtttc gttcctccca gcacacggaa acacatgact
     2521 ttcttggcgc ccgtattatc ggcgacattc agcctggaat attcctgtat cattttactt
     2581 ggccttctcc aaaatcttta cgagtcgcca gcgtttcatt ttcgagagcg ggcgagtttc
     2641 cataaccatt accttatcgc caataccagc ctcgttattc tcatcatgag catagagctt
     2701 ggcgcgcttg tgaatgacct tgccgtacaa aggatgcttc atcttgtagt ccatctcaac
     2761 cgaaatggtc ttgtccatct tgtcggatat cacaataccc tgtctcgttt tccgtagtcc
     2821 tctcaatgcc acagtttact cctctgaact cttctctttg ccgctgccca gtatctttcc
     2881 ggttgtggcg gaaagcgcgc ggataccttt ttcatgctca tgaaggactg tcttaagacg
     2941 ggcaatatcg cgccgtaaga ctcgcaggcg caggggatta tcgagcttct ggacccgcat
     3001 tttaagcctc aggttgaaaa gctcttcctg ggagtcggcg agccgctgct cgacttcttc
     3061 cttcgccaga tctctgatta attccgcctt cacggtacta tgctcctcca atttcctgac
     3121 gggtaacgaa tctgcacttg atcggcagct tgtcggcggc gagtttcatc gcctggcgag
     3181 caagctcctc ggagacacct tctaattcaa acagaatccg tcccggctta accaccgcca
     3241 cccagtattc gggcgctccc ttacccttac ccatacgggt ctccgcaggc ttcttggtaa
     3301 agggcttgtc cgggaagacc ctgatccaga ccttgccgcc tctcttgatc ttccttgtta
     3361 acgccacacg tgccgcctct atctggcggt ttgtgatcca ggccggcgcc atagctttga
     3421 ggccgaactc gccaaatgag atcttcgagc cgcggtaggc cttgcctcgc attctgccgc
     3481 gctgctgctt gcggtatttt actctttttg gcattaacat atgctattac tccgaatcgt
     3541 tcccgttcaa gaacgtgaaa atttccaaat atatgtacat tcgatcaaaa ggcaagagct
     3601 tttgtatatg aagtcttacc cttttttgtc ttctgattta tctcccgatt ccggcctgga
     3661 ggccgtcctc ttggcttcgg tcgaagctct tggctctggt ctgccctggt ttctttgttg
     3721 ctgctgccct tcggctctgc gtccgccttc atcctcaggt cttttcttgc ggaccctgga
     3781 tttggggcga ggtttacgcc tgcgcggccc cgaggggcgg gctcttctct gatccatttc
     3841 gggctggcgg ctttcacccg catcctccat gcccggctta tccagaattt cgccgcggca
     3901 gatccagacc ttcacgccga tagtgccata agtcgtatta gcagttgaaa tagcgtagtc
     3961 tatatccgcc cggagagtat gaagcggaac ccgtccctcc atatacttct cgcttctggc
     4021 aatttccgca ccgcccagac ggcccccgca ggtaatcttg ataccctcgg cgcccatctt
     4081 catcgaagcg gtgatagcct tcttcattga acgtctgaag gatactcgtc cctccagctg
     4141 cctggcaatg gaatcagcca ccagcttcgc gttcagctca ggctttttca cctctataat
     4201 attcaaggta atatctttcc tggtaattaa ctgcagctct tcacgcaatt tatcgacttc
     4261 cgctcctttg cgcccgatga cgatgcccgg acgggaagaa tgaatgtcaa ctgtaatttt
     4321 cttgggagca cgcaggattt caactttggc aagcccggca ttttccagac gcttgctaat
     4381 ataccttcgg atcttcagat cctcctcgag aagatctgca aagttcctct ccgcaaacca
     4441 cttggagttc caagatctga tgattcccag cctcaatccc actggattag ttttctgacc
     4501 caaaagtatt cctcctgcta ttcttcttct tccgccacgg aaggtttagt tggttttctc
     4561 tttcttgtgc gcctggcgtg ttccctggcc agacgtcttt ccgcacggcc ctcatcggat
     4621 accctgaccg aaagatggca ggtacgtttg cgtattctga aggcccggcc catgccgacg
     4681 gcgcggaagc gcttcaggat aggccctccg tcaatgatga tagaggttac tctcagttcc
     4741 tcagctttca cccgggcagt gccttcctgg gcgatcatat tggcggcagc cgattttatg
     4801 gttttggaaa ggggcaccgc ggccctcttg ggcacatacc tcagaaggtc aactgcctcg
     4861 tgaaccatct ttcctttcac cagctccgcc acccgccgca tcttgcgcgg ggacattctc
     4921 aggtattttg aacgagcaac agattccata attccactct tactttatcg aagatgacct
     4981 ttcggttaac ttgccggcat ggccgcggaa aatccgggtc ggagcaaatt cgcccagctt
     5041 atgaccgacc atattttctg taatatatac aggaataaac ttgttgccgg tatgaatagc
     5101 aattgtatgc ccgacaaatt ccggcgatat ggttgacctg cgcgaccagg tcttgataac
     5161 ctttttgtcg ccgctgtcat tcatcttctc gatcttgatc attagatgat catctataaa
     5221 gggccctttt ttcaaagagc gtgccattca gaacctccta aaac
//
LOCUS       DSEB01000192            1592 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1016772, whole genome shotgun sequence.
ACCESSION   DSEB01000192 DSEB01000000
VERSION     DSEB01000192.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1016772"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            94..1350
                     /locus_tag="ENO07_03555"
     CDS             94..1350
                     /locus_tag="ENO07_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01073.1"
                     /translation="MNYAKMRLITIMAIVLVLSPPAFAEDVHISGFLQGLYGGGLDDD
                     NPTPSELTAAETRLQLKLESYGDMAEFFGRLDFVYDGYDEPEYDWELREGYIKFRLGN
                     NFDFKVGRQVLTWGTGDLIFINDLFAKDYVSFFIGRDDQYLKAPQDALRGAYYSPIGT
                     FSLVYTPRFAPNRVPTGMRLSYFNPMAGDIVGGQEFYFEGRLPQAEIENGEIAGRFQR
                     YFGTADFALYGYRGFYKNPEGFDPTAMQAYYPRLNVYGASIRMPVFEGIAWLEGGYYD
                     SREDTDGDNPNIPNSSLTGLVGFERQIVTNLTVNMQYQGEIMMNYDEFVESIGGQTEV
                     DEIYHLLTSRVTKLMRMETVVLSLFGFYSPSQEDFYGCFSLDYKYTDNLTLTVGGNIF
                     AGEDQFTDFGTFQKNDNLYLKITYGY"
     gene            1445..>1592
                     /locus_tag="ENO07_03560"
     CDS             1445..>1592
                     /locus_tag="ENO07_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015879610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HDS01074.1"
                     /translation="MTLENAVRMFAGFMVLLSVALTYFVSPWWMLFTIFIGFNLIQSS
                     ITGLC"
BASE COUNT          394 a          368 c          409 g          421 t
ORIGIN      
        1 actataatgt aggcgtgacg gatgatatct tcaccgagag atatctcaag aatcccccgc
       61 gcgaatatat aaaataaaag gtggaggtgc ggtatgaact acgcaaaaat gagacttatt
      121 acgattatgg cgattgttct ggtgctgtcc ccgccggcct ttgccgagga tgttcacatc
      181 agcggatttc tacaggggct ctatggcggt ggtttggatg acgacaaccc gactccctcg
      241 gaacttaccg cagccgaaac ccgtctccag ctcaagctgg aatcttacgg cgacatggcc
      301 gagtttttcg gccgcctgga ctttgtttat gatggatacg acgagccgga atacgactgg
      361 gaacttcgtg agggctacat caaattccgg ctcggcaata attttgattt caaggtcggc
      421 cgccaggtgc tcacatgggg caccggcgac ctgatcttta tcaatgatct gtttgccaag
      481 gattatgtct cgttttttat cggccgggat gaccagtacc tgaaagctcc tcaggatgca
      541 ctgcggggtg catattacag ccccatcgga accttctcgc tggtctatac tccgcgtttt
      601 gctcccaaca gggtcccgac cggaatgcgc ctgagctatt tcaatcccat ggccggtgat
      661 attgtgggcg gtcaggaatt ctatttcgag ggcagacttc cgcaagcgga aattgaaaac
      721 ggagaaattg ccggcaggtt ccagaggtat ttcggtactg ccgattttgc gctctacggt
      781 taccgcggat tttataaaaa tcctgagggc tttgatccaa ccgcaatgca ggcctattac
      841 ccgcgcctga atgtttacgg cgcctcgatc cgtatgcctg tgttcgaagg aattgcctgg
      901 ctggaggggg gctactacga ttcgcgcgag gatacagatg gtgataaccc caatataccg
      961 aattcatcgc tgacaggcct ggtcggattt gagcgtcaga ttgtaaccaa cctgacggtc
     1021 aatatgcagt accagggcga gattatgatg aattatgacg agtttgtcga aagcatcggc
     1081 gggcagaccg aagtcgatga gatctatcat ctgctgacat cgcgagtgac taagctcatg
     1141 cgcatggaaa cagtggtatt gtcattattc ggattttatt cgccttcaca ggaggacttc
     1201 tacggatgct tttctctgga ttataaatac actgataatc ttacgcttac ggtcggcgga
     1261 aatatctttg caggcgagga ccaattcacc gacttcggga catttcaaaa gaacgataat
     1321 ctctacctga aaataacata tggctattaa aaatgaccgg atgtgatgga acaaggcaac
     1381 gcagatatcg tttagaaata taccggataa taattgtatg cattatagat gaggagagta
     1441 tagcatgaca ttagagaacg ccgttagaat gtttgccggt tttatggtcc ttttatcggt
     1501 ggccctgacc tatttcgtgt ccccatggtg gatgctgttt acgatcttta tcggattcaa
     1561 cctgatccag tcctcgatca ccggtttatg cc
//
LOCUS       DSEB01000193            3614 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002732, whole genome shotgun sequence.
ACCESSION   DSEB01000193 DSEB01000000
VERSION     DSEB01000193.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002732"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            163..1113
                     /locus_tag="ENO07_03565"
     CDS             163..1113
                     /locus_tag="ENO07_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01075.1"
                     /translation="MQDRNQEYRQAVLMAAIVAVVPLIFYPRSLGLALSISPLLFLLI
                     ELVFYWIIFSAFLKGATFNNIFISGTICLFSRLVTGAVFALLVVLMYAAPPAEAFAAG
                     IFKYKPAMLLQVISFPFILMPALKRLLDLQTEPKTQAVFQTADQISAEPPPQKPKAAA
                     DDMKDRKIIGLKSAAAEKPFSGFDEALKYVGETSAVRFAVLIDSRGLPVSFFGNNKSL
                     RDLWSAVGKFLVDELDEPLRRAGDFEFEGFELTLNTYRIHVVKAEELYLFVAADKESG
                     ETEKVRVSQAANMVRRIYQERYSIKPDINAREESYVRSFS"
     gene            1076..1429
                     /locus_tag="ENO07_03570"
     CDS             1076..1429
                     /locus_tag="ENO07_03570"
                     /inference="COORDINATES: protein motif:HMM:PF03259.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01076.1"
                     /translation="MLGRKVMYEVLADLNRTSGVTGSMLVGMDGIIIAADMDTVDKDE
                     AVGALAASITSSVKKSLERLKHHELEQVTIEAEGGNLFLTASKPGILVVKTEEDVNIG
                     LVRLEIRNALNRLES"
     gene            1451..2779
                     /locus_tag="ENO07_03575"
     CDS             1451..2779
                     /locus_tag="ENO07_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01077.1"
                     /translation="MVAINYANKEVSCKIVYYGPGLSGKTTNLIYIHSKVPGTTRGDL
                     ISLATDADRTLYFDFLPINIGTINGFTTRFQLYTVPGQVFYNATRKLVLRGVDGLIFV
                     ADSQRDKADENVESLNNLIENLQEYGYDLDKIPMVLQYNKRDLPDIMSIEELNQLLNS
                     RNWPVFESSAHKGTGVFDTLKLIIKMILEKAKKSDTAKKIEESKTEHVKQPSAEQPVP
                     QTIPKPSEPQTDKIPIQKEPEPEAQRPAADSRPDSPAPTPAPRPEPEHSSFERAEPVH
                     TPERSYSEPHKVAASAESGATAAALADRPQTENRENEEQISSRPTNEEIDPGPDEGFE
                     ENVMADNGTEEAAQAHHTAEETVKPDVDQQFAETEAVPEKESQSADADEEPEDIEEVS
                     AEEPEQEDAEVDEEMFELPSNRPKMAASVRLRQDKKKKKGFFLFRLFGKK"
     gene            2755..3285
                     /locus_tag="ENO07_03580"
     CDS             2755..3285
                     /locus_tag="ENO07_03580"
                     /inference="COORDINATES: protein motif:HMM:PF03259.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HDS01078.1"
                     /translation="MPAVRQKIDQEVKKVAGDGLIVYQEETAKIDGALRKLLKGSEAK
                     CALLVDKDGHLITRQGFTHSLDTTALAALLAGSFASTKEIARLVGEPEFSVLFHQGKR
                     DHIHMSLINERTILVVIFDDRTTIGMVRLYSKETAEELNQIFELALSKKGADDDGLSD
                     DFADSAESKLDDIFQD"
     gene            3302..>3614
                     /locus_tag="ENO07_03585"
     CDS             3302..>3614
                     /locus_tag="ENO07_03585"
                     /inference="COORDINATES: protein motif:HMM:PF05157.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01079.1"
                     /translation="MPGDLGTMLVTAGKITDEQLKKAQQIAKSGEVRLDQALIRIGAL
                     DDTDEISAFIGKQLNIGALRLQDIELNPEVVKLIPLDIARKFNVIAVSKLGKTLVVAI
                     SD"
BASE COUNT          961 a          847 c          997 g          809 t
ORIGIN      
        1 aacaggttct tatcgagctg gaaggtgaaa aaatctggat gatccatacc ggcaatcaga
       61 ttctggtact ggtaatgtcg gagaatgtca acctgggttc actgaaactg aagctttcca
      121 aagttttggc tcagatagcc gagatataac tgaggggaaa atatgcaaga tcgaaatcag
      181 gaatacaggc aggcagtcct tatggccgca attgtggcag tggtgccgtt gattttttat
      241 ccgcgaagtc tggggctggc gctgagcatt tcgcctttgc tgtttttgct gatagagctg
      301 gttttctact ggatcatatt ttcagctttc ctgaaaggcg cgacgttcaa taatattttt
      361 atttccggga cgatctgtct cttctcgagg ctggtgacag gcgcggtttt cgcgctcctg
      421 gttgtgctca tgtatgccgc cccgccggct gaagctttcg cggcgggaat attcaagtac
      481 aaaccggcca tgcttttgca ggttatctca ttcccgttta ttctcatgcc ggctctgaag
      541 cggcttctgg acctgcagac cgaaccaaag acacaggcgg tttttcagac cgctgatcaa
      601 ataagcgccg agcctccgcc gcaaaagccc aaagctgccg cggacgatat gaaggaccgc
      661 aaaatcatcg ggctcaaatc agccgcagcc gaaaaaccat tcagcggatt cgatgaggct
      721 ctgaaatatg tgggggagac ctcggcagtg cgctttgcag tcctgatcga ttccagggga
      781 cttccggtgt cgtttttcgg caataataag agcttgagag atttatggtc tgctgtggga
      841 aagtttcttg ttgatgagct tgacgagccc ctgcgcaggg ccggggattt cgagtttgag
      901 ggattcgaac tgaccctcaa tacatacaga atacatgtcg tgaaagccga agagctttat
      961 cttttcgttg ccgccgataa ggaatcgggc gagaccgaga aagtgagagt gtcccaggcg
     1021 gccaatatgg ttagaagaat ttatcaagaa cgatacagca ttaagcccga tattaatgct
     1081 cgggaggaaa gttatgtacg aagttttagc tgatttgaat cgcacctccg gggtgaccgg
     1141 gagtatgttg gtggggatgg acggtatcat tatcgccgcc gatatggata cggtcgataa
     1201 ggacgaggct gtcggtgcac tggccgcctc gatcaccagt tcggtaaaga agtcgctgga
     1261 gcgcctgaaa caccatgaac tggagcaggt caccatagaa gctgaaggag gcaacctgtt
     1321 tttgaccgcc tccaagccag gcatacttgt ggttaagaca gaggaggatg tgaatatcgg
     1381 cctggtcagg ctggaaattc gcaatgcatt gaaccgttta gagtcctaat gaaaggctgg
     1441 gcttgagaat atggtcgcaa ttaattatgc caacaaagaa gtcagctgca aaatcgttta
     1501 ttatggaccg ggtctctcgg gtaagaccac gaacctgatt tatatccata gcaaggttcc
     1561 tggtacgacc cgcggcgatc tcatcagcct ggcgacagac gccgacagaa ctctgtattt
     1621 cgatttcttg cctatcaata taggtacgat taacggcttt acaacgcgct ttcagctcta
     1681 tactgtgccg ggccaggtct tctataacgc tacccgtaag cttgtgctgc gcggggtgga
     1741 cgggctgatc tttgtggccg acagccagag agacaaagct gatgaaaatg tggagagctt
     1801 gaacaacctg atagagaacc tccaggaata cggatacgac ctggacaaaa tacccatggt
     1861 gcttcaatat aataagcggg atcttcctga tatcatgtcg atagaggagc tcaatcagct
     1921 tctgaacagc aggaactggc ccgtattcga aagctcggcc cataaaggca cgggcgtttt
     1981 cgatacattg aagctgataa tcaagatgat cctggagaaa gccaaaaagt cggatactgc
     2041 caagaagatc gaggagagta aaacggagca cgtgaaacag ccgtcagctg agcagcctgt
     2101 tccgcagact attcccaaac caagcgaacc gcagaccgac aagattccga ttcagaaaga
     2161 accggagcct gaagcgcaga ggccggcggc cgattcccgg ccggacagtc cggcgcccac
     2221 tccggctccg cggccggaac cggagcactc ctcattcgag agagcagaac cggttcatac
     2281 tcccgagcgg tcatactccg aaccgcataa ggttgcggcc agcgccgagt ccggcgcaac
     2341 ggcggcggcc ctggcagaca ggccgcagac tgaaaaccgg gaaaacgaag agcagatctc
     2401 cagccgcccg accaatgagg aaattgaccc cggtccggat gaaggtttcg aagagaatgt
     2461 tatggctgat aacggtaccg aggaagcggc ccaggcccat catacagcag aggagaccgt
     2521 gaaaccggat gtggatcagc agttcgctga aactgaagca gtgcccgaaa aagagtccca
     2581 gtctgcagac gcagacgagg aaccagaaga tatagaagaa gtaagcgccg aagagcccga
     2641 gcaggaagat gctgaagttg atgaagagat gttcgaactt ccttccaacc ggcccaagat
     2701 ggccgcctcg gtgagattga gacaggataa gaagaagaaa aaaggtttct tcttattccg
     2761 gctgttcggc aaaaaataga tcaggaggta aaaaaagtgg caggtgacgg tttgatagtc
     2821 tatcaagagg agactgccaa aatcgacgga gctctcagga agctcctgaa gggctccgag
     2881 gccaagtgtg ctcttctggt tgataaagat ggccacttga ttaccaggca gggattcacc
     2941 cattcgctgg atacaactgc tctggcagcc ttgctggccg gttcatttgc ttccaccaag
     3001 gagatcgccc gtctggttgg agaaccggaa ttttccgttc tctttcatca gggcaaaagg
     3061 gaccatatcc atatgtctct catcaacgag agaacgatac tggtggtgat ttttgacgat
     3121 cgcaccacta tcggcatggt ccgtctttat tcaaaggaga cggcggaaga gcttaatcag
     3181 atttttgagc ttgcgctctc gaagaaaggc gctgatgatg acggcctttc ggatgatttt
     3241 gccgactccg ctgaaagtaa attggatgac atttttcaag attaattagc agaaggaaga
     3301 aatgcctggc gatcttggaa caatgctggt tactgccggc aagatcacgg acgaacaact
     3361 aaaaaaagcg cagcagattg ccaaatccgg cgaggtgcgc ctggatcagg cactgatcag
     3421 gatcggcgcg cttgatgaca ccgatgaaat ctccgctttt atcgggaaac agttgaatat
     3481 cggcgccctc agacttcagg atatcgaatt aaatcctgag gttgtcaagc ttattccgct
     3541 tgatattgcg cgtaagttca atgtgatcgc cgtcagcaaa ctgggtaaga ccctggttgt
     3601 ggccatatcg gatc
//
LOCUS       DSEB01000194            2634 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1005488, whole genome shotgun sequence.
ACCESSION   DSEB01000194 DSEB01000000
VERSION     DSEB01000194.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1005488"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..183)
                     /locus_tag="ENO07_03590"
     CDS             complement(<1..183)
                     /locus_tag="ENO07_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01080.1"
                     /translation="MAEIQKNKKAIGFKDDPMMPRQFMIQRFRRDTVDTFSMELKPVS
                     GPKKFDFKPGQFNMIYV"
     gene            complement(161..628)
                     /locus_tag="ENO07_03595"
     CDS             complement(161..628)
                     /locus_tag="ENO07_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HDS01081.1"
                     /translation="MESLAKVLKEHPFLEGLEQRHIDFITGCGSNVVFKKDEFLFREG
                     DEANHFYFIRHGKIIIETYIPQKGPIAIQTRETGDVTGWSWLIPPYRWHFDARAVELT
                     RAIALDGKCLRGKMDEDHELGYELMRRFARLMAQRLEATRMQLFDVYGGNTKE"
     gene            complement(633..1769)
                     /locus_tag="ENO07_03600"
     CDS             complement(633..1769)
                     /locus_tag="ENO07_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit A"
                     /protein_id="HDS01082.1"
                     /translation="MILVLESGNLQSLFDTLISEKYQIIGPTLDNSALIYDEITNVDD
                     LPVGYTDEQDGGRYRLVKSGGKKYFDYVVGQHTWKRYLYPPEKKLFELKRKGDGFEIV
                     ENKQEIHKRAFIGVRACEIEAIRIQDEILLEGPYKDADYERCRQNIFIVAVNCVRPGG
                     TCFCVSMGTGPRATKGFDLAITEIVDNSGHYFLIESGTKAGEKIMKEIKGKEADQNAI
                     ETADRLMKEAENKMGRRLDTDDIKELLYRNFESSRWEVVAQRCLNCGNCTLVCPTCFC
                     INIEDISGLEVNSAKRVRKWDSCFTLDHSYIYGGSVRTSAKARYRQWLTHKLASWIDQ
                     FGMSGCVGCGRCITWCPVGIDITEEVQAIRESEGKGKIMADKEE"
     gene            complement(1923..2126)
                     /locus_tag="ENO07_03605"
     CDS             complement(1923..2126)
                     /locus_tag="ENO07_03605"
                     /inference="COORDINATES: protein motif:HMM:PF04358.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01083.1"
                     /translation="MKIPGGKLSERHWQIIHYLRDRFAKKNEIPTVYETCEDNKIDLD
                     DLERLFPDGYHRGAVKISGLRII"
     gene            complement(2148..2618)
                     /locus_tag="ENO07_03610"
     CDS             complement(2148..2618)
                     /locus_tag="ENO07_03610"
                     /inference="COORDINATES: protein motif:HMM:PF04358.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01084.1"
                     /translation="MLRFSDFNKEPVMSIVNYKDKTYSVDSHDFLIDFFIWDEDFAEW
                     TAMQLGMPPKLTEEHWDVIYYVRLEFQEKGKCPTVYETCRMCKLGLSDLLRLFPSGYL
                     RGACRIAGVTYKQGYLGQNFMPTTEKDYDVIATEKVYQVDVRGFLVNPDDWDEF"
BASE COUNT          676 a          690 c          530 g          738 t
ORIGIN      
        1 gacatagatc atattgaact gcccgggctt aaaatcgaac tttttcggtc cggagacagg
       61 cttcagttcc atggaaaatg tatccacggt atcgcgtctg aagcgctgaa tcatgaactg
      121 ccggggcatc atcggatcat ctttaaatcc gattgccttt ttattctttt gtatttccgc
      181 catacacatc aaatagttgc attcgggttg cttccaggcg ctgggccatg agtctggcaa
      241 aacgcctcat taattcataa ccgagctcgt gatcttcatc cattttaccg cgcaggcatt
      301 taccgtccaa tgcgatcgct ctggttaatt cgaccgcgcg agcgtcgaaa tgccagcgat
      361 agggcggtat caaccatgac caaccggtga catccccggt ttcacgggtc tgaatcgcta
      421 tcggaccttt ttgcgggata taagtttcaa ttataatctt accgtgcctg ataaaataga
      481 aatggttagc ctcgtcccct tcacggaaaa gaaattcatc cttcttgaaa acaacattgg
      541 agccgcagcc ggtaataaaa tcgatatgcc gttgctccaa tccctccagg aatggatgct
      601 cttttagtac ttttgccaga gattccataa atctattcct ctttatctgc cattattttg
      661 ccttttcctt cactttccct gatggcctga acttcctcgg taatatcgat tcccaccggg
      721 caccaggtaa tgcatctgcc gcaaccgaca cagcccgaca tcccgaattg atcgatccaa
      781 ctcgccagtt tgtgagtcag ccattgacga taccgagcct tggctgaagt gcgcacactt
      841 cctccgtata tataggaatg atccagtgta aaacatgaat cccatttgcg cacacgtttg
      901 gccgaattga cctcgaggcc ggagatgtcc tcgatgttta tgcagaaaca ggttgggcac
      961 actaaagtgc aattgccgca attcaaacat ctctgcgcaa ctacttccca gcgggaactc
     1021 tcgaaattgc ggtaaagcag ctcctttatg tcgtctgtat cgagccgccg gcccatttta
     1081 ttctcggcct ctttcatcag ccggtcggct gtctcgatcg cattttgatc agcttctttg
     1141 cccttgatct ccttcatgat tttttcgccg gcctttgtac cggattcaat caagaaatag
     1201 tgaccgctgt tgtcaacaat ttccgtgatc gccaggtcaa atcccttggt ggccctggga
     1261 ccagtaccca tcgagacaca gaaacaggtg ccgcccggac gcacacaatt cacagcaaca
     1321 atgaaaatat tctgtcggca tctttcataa tccgcatcct tatacggccc ttcaagaaga
     1381 atctcatcct ggatccggat cgcctcgatt tcacaggcgc ggacgccgat aaatgcccgt
     1441 ttgtggattt cctgcttatt ttccacaatc tcgaagccgt cacctttacg cttcagctca
     1501 aacagcttct tctcaggcgg ataaagatat cttttccagg tatgctggcc gacaacataa
     1561 tcaaagtatt tcttgccgcc cgattttacc agtctatatc ttccgccgtc ctgttcatca
     1621 gtatacccaa ccggcaaatc atccacgttt gtaatttcat catatataag ggccgaatta
     1681 tccagggtgg gaccgattat ttgatatttt tctgaaatca gcgtatcaaa aagactttgc
     1741 aggttaccac tttcgagaac cagaatcatc cgccaaatcc ttcaatcgac attgtgattt
     1801 atttcacaat ttatactcaa aatatgattg tattcattga ctgtcaagaa gaaaaataga
     1861 ttaatctatt tcatgctgta tctgttagat accgcattca tgagctcacg gcaacaagaa
     1921 ctttaaatta ttcttagacc ggaaattttt acagccccgc gatgataacc atcgggaaat
     1981 agtctctcaa gatcatccag atcgatttta ttatcttcgc aggtctcata aacggtcgga
     2041 atctcattct ttttggcaaa cctgtctcgc agataatgta ttatctgcca gtgcctttcg
     2101 gacagtttgc cgcctgggat tttcatatct gcagcgcgat gggcggccta aaattcatcc
     2161 cagtcatcgg ggttgaccaa gaatccgcgc acatcaacct ggtatacttt ttcggtggca
     2221 atcacatcgt aatctttttc ggtcgtgggc atgaaattct ggccgagata accctgttta
     2281 taggttactc ccgcaatcct gcaggccccg cgaagatagc cggatggaaa cagtcgcagc
     2341 aggtcactga gaccaagttt gcacatgcgg catgtctcat aaactgtcgg gcatttccct
     2401 ttttcttgaa actcaagcct gacgtaataa atcacatccc agtgctcctc ggtcaacttg
     2461 ggaggcatgc cgagctgcat ggcagtccat tcggcaaaat cctcgtccca gatgaagaaa
     2521 tcgatcagaa aatcatgaga gtcaaccgag taagtcttat ccttataatt tacaatgctc
     2581 atgactggct ccttgttaaa atccgaaaaa cgcagcatta aaccagccgc aaac
//
LOCUS       DSEB01000195            1850 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1012067, whole genome shotgun sequence.
ACCESSION   DSEB01000195 DSEB01000000
VERSION     DSEB01000195.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1012067"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            150..1253
                     /locus_tag="ENO07_03615"
     CDS             150..1253
                     /locus_tag="ENO07_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HDS01085.1"
                     /translation="MPDNNEELRKAGKLLYPIFREYVAVEAIYHRGEDLIFKTVPKVP
                     LEDFQDKLSKQAFEAGFYLAFFQDGDSLLIVAKDIRPQGTKIPWLNILLFVLTLASMI
                     IAYGYIKYESEIFRDFGLMAEGIKFALALMPILLFHEFGHYLAARRNKADVSLPYFIP
                     APTLIGTLGAIIKSRSPFKTRRELFDVGAAGPLAGFVPAVIILAIGFSNAQIINIGDQ
                     PLGTGHLLMGQSLVYYFLKILIVPPIPEGYTLIMSPTIFAGWVGLFVTMINLMPVGQL
                     DGGHITYALFGRKLQFYLAWIIVAGLVVMGFFWPGWFVWGFLAVVIIKLKHPPTMNDE
                     VELDQKRVILGWVSIALFILTFIPIPLKFVIVQ"
     gene            complement(1263..>1850)
                     /locus_tag="ENO07_03620"
     CDS             complement(1263..>1850)
                     /locus_tag="ENO07_03620"
                     /inference="COORDINATES: protein motif:HMM:PF01966.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HDS01086.1"
                     /translation="RDNYTRGHTERVYRIASMIVRALGWSEEQMKNLYMGCILHDIGK
                     IGVPDSILNKPGRLNDEERSIMQMHPETGVKMLEGIPMLEPALPYILYHHEQYDGSGY
                     PLGLKGGNIPIEGRILAIADTVDAILTNRPYRRGASLEKVSRELIECSSGQFDPELVK
                     IFLNVLGEHKDELTAIYGTLQEHENNILTPQSAAQ"
BASE COUNT          376 a          501 c          488 g          485 t
ORIGIN      
        1 actatctgtt catgggcggt ccgctgccga tgggcgactg ggttgtcagg taattcatcg
       61 attctgatag attcagggaa agcaagtttt gcttgctttc cctttttttt tgcttagatt
      121 tgatttttta acctggaacc gatacctcta tgccggacaa taacgaagaa ctccgcaaag
      181 cgggcaagct gctctacccc atcttcagag aatatgtggc cgtggaagca atctatcacc
      241 gcggcgagga ccttatcttc aaaaccgttc ctaaagtgcc cctggaggat ttccaggaca
      301 agctctccaa acaggccttc gaagccggtt tttacctggc attttttcag gacggcgatt
      361 cgctcctgat cgtggccaaa gatatccggc cccagggcac caaaataccc tggctcaaca
      421 tcctgctctt cgtcctgacc ctggcctcca tgataatcgc ctacggttat atcaaatacg
      481 aatccgagat cttccgggat ttcggcctta tggcggaggg aatcaagttt gcgctcgccc
      541 tgatgcccat cctgctcttc catgagttcg gtcattacct ggctgcgcgc agaaacaagg
      601 ctgatgtcag cctgccctat tttatccccg ccccgaccct gatcggcacc ctgggggcga
      661 tcattaaatc ccgctctccc ttcaagaccc ggcgcgagct ttttgatgtg ggcgcggccg
      721 gaccattggc cggtttcgta ccggcggtga taatcctggc gattggtttt tccaatgcgc
      781 agatcatcaa catcggcgac cagcccctcg gaacggggca tctcctgatg ggtcagtcgc
      841 tggtctatta tttcctgaaa atattgatag tgccccccat acccgagggc tatacgctca
      901 tcatgtcccc caccatattt gccggatggg tggggctgtt cgtgaccatg ataaacctaa
      961 tgccggtggg acagctcgac ggcggtcaca taacctacgc ccttttcggg aggaagctgc
     1021 agttttacct ggcctggatt atcgtagcag gtttggtggt tatgggcttc ttctggccgg
     1081 gctggtttgt gtggggcttt ttggctgtgg tcattataaa attgaagcat ccgcccacca
     1141 tgaacgacga ggtcgagctg gatcagaagc gagtaatcct gggctgggtc tcgatcgcgc
     1201 tgttcatctt gaccttcatt ccgattccct tgaagtttgt gatagttcag tgaacgcggg
     1261 atttactggg cggcggactg gggtgttaga atattgtttt cgtgctcctg cagggtgccg
     1321 taaatcgcgg taagttcatc cttatgttcc ccgagcacat tcaggaaaat cttgaccaat
     1381 tccggatcga attgcccgga actgcactcg ataagttccc gggaaacctt ctcgagtgaa
     1441 gcgcctctgc gatagggacg attggtcagg atggcatcga cagtatcggc tatggccagg
     1501 attctcccct cgataggaat attgccgccc ttgagcccca gcgggtagcc gctgccgtcg
     1561 tactgctcat gatgatagag gatatagggc aaggccggtt ccagcatggg aatgccctcc
     1621 agcatcttca ctcccgtctc gggatgcatc tgcatgatac tgcgttcttc gtcattgaga
     1681 cgtcccggct tattgaggat tgaatcgggc acaccgattt taccgatatc gtgaagtatg
     1741 cagcccatgt agagattctt catctgttcc tcggaccagc ccagcgcccg gacgatcatt
     1801 gaggcgatcc tgtagaccct ctcggtatga ccgcgggtgt agttgtcacg
//
LOCUS       DSEB01000196            5340 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001131, whole genome shotgun sequence.
ACCESSION   DSEB01000196 DSEB01000000
VERSION     DSEB01000196.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001131"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            91..2403
                     /locus_tag="ENO07_03625"
     CDS             91..2403
                     /locus_tag="ENO07_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01087.1"
                     /translation="MYRKALFLLFLIISFNAPLVGQFVDPGIPDTIRVDSVQIDPGPA
                     PVTFSVEVTLFNDEELAAASLGFYYDSDDIEIDSISLIGGAAEHLPNLSFASQDSNLA
                     AVGFYWPGPFFDYVSTGDSLLATLWFTLAANAVDQTINIDSGYFAPAGDFILTPTTGI
                     SLKPQFKPGKVVIGEGTPPQPEIGLNPTSFNFYAEVGGSNPTPKVLNIVNVGDGTLNW
                     TASWSSSWLTVSPSSGTAPSNPSVSINIFGMTAGTYEDTITIADPNAVNSPQYAPVTL
                     QLVNPPPEIQLVPDNFSFIAVQDGANPPNQQMTINDVGGGTLSWTASNSSTWLTLSSY
                     AGGPGETIDLIIDITGLTYGLYYDTIVVSDPEASNSPQLAEVVLELVSSFPVLEVLPD
                     SFVVAGSVSEDPYDHVLKVINGGGGTMNFELTADQPWISFNPQTGSTTDTAEVLVTFH
                     SAGLPLGFQYATITVSSDNASESPQEVGVQLWVMEDPPALGVSTNTLSFSGFPCQNIP
                     PIAGQSFEITNPSPNDLVWSAEWDASWLSLSPASGYSETTVNVFVDEFGLEPGTYVDT
                     VKIDAVWSSTPDEYIEVSFTLVENPSPPELVLSTDFVEFIFLAGYVGASSASKLYIDN
                     AISGCMDWYITDPYPWLNFDKNSGVAPDSVISAVNGGGLPLGITPGQFTVHAPGSSND
                     SIVVNFNVYIAQLGDANCNGQINMSDVVYIVNYIFVSGPTPIPRMWAGDVNCDFRANI
                     EDAVLLVSYIFGLGLDICQYNPIVNPNPDK"
     gene            2744..5041
                     /locus_tag="ENO07_03630"
     CDS             2744..5041
                     /locus_tag="ENO07_03630"
                     /inference="COORDINATES: protein motif:HMM:PF01433.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01088.1"
                     /translation="MEVPVKSIYILAIIASLLLGISTASADGPEEARAFFEKINDNDL
                     IIEDISRLTPEELDEMIETTHDFCRYGKALRLQAYRDMADQGLIKSATAAQNDWDALY
                     YELNFGIDFGTEIIDGYVGLQAASLVPSLSFIELDFLDNMIVDSVIKDGSPLTFSHAD
                     NLITITLDNTYDSSETFNLKVYYNGHPTEGGFQAFEFGYHGATPIVSTLSEPYFARSW
                     WPCKDYPHDKADSVDIIITHPDEYICASNGLVQSIVDNGDGTKTTHWKHNYPITTYLV
                     AIGVTNYIEFGDWYVSEPGDSMPVNFYVYPENYSQAVSSFPITVDMIDTLSAIFGEYP
                     FIDEKYGMLHFGWGGAMEHQTNTSISSSAYYQSIIVHELAHQWWGDMITCENWHEIWM
                     NEGFATYSEALWFEATQGDSFYHVYMDDMAYTAGGTIWCQDTTSVWSIFTSRVYDKGA
                     WVLHMLRNWVGDSTFFEILTTYYDDPRYKWKTINTEQFRDLCIEVSGDSRLTEFFEDW
                     IFGEYYPQYRFSYIFEEYQPGDYIVYLHLRQTQATNPQVFDMPIELSFFDGTNYHETT
                     IYNDQREQDFIVYLDDLANDPYLLWLDPDNWILKNSAMEAYGLNLIDSPLDTAYQYQD
                     YQDSVIARGGTSPLTYYQVGGSLPSGVALYPNGKVLGTPLSYGSFDFKVRVEDSGYPT
                     KVDTLTQSLTVLEFVPLVGDANFDGLVNVSDAVHIINYVFIGGDPPQPILEVGDVNCD
                     STVNISDAVWIINYVFVGGPAPGDC"
BASE COUNT         1283 a         1240 c         1308 g         1509 t
ORIGIN      
        1 acaatataca gtggacattt ctttcctgaa aggtaaacct taggatttac ttcgataaca
       61 tggaaagttt ataaaattgg gaggtgttga atgtatcgga aagctctgtt tcttttattc
      121 ctgattattt ctttcaatgc gccccttgtg gggcaattcg tggatcccgg catcccggat
      181 acgataagag ttgactcggt tcagatagat cccgggccag cgccggtgac gttcagtgtg
      241 gaggtaacgc ttttcaatga tgaggaacta gcggctgcct ctcttggatt ttattatgat
      301 tctgatgata ttgagatcga ctcgatatct cttatagggg gagcggctga gcatcttccc
      361 aatctgtcat ttgcctcgca ggattccaac ctggcagcgg ttggattcta ttggccgggt
      421 cccttctttg attacgtttc taccggggac agcctgctgg caaccttatg gtttacgctg
      481 gcagcaaatg ctgtcgacca gacaataaat atagactcag ggtacttcgc tcccgctggt
      541 gatttcatcc taactccgac caccggaata agtctgaagc cccagtttaa accaggcaaa
      601 gtcgtaatcg gggagggaac tccgccacag cccgagatcg ggctcaaccc gacctctttc
      661 aacttctatg cggaggtcgg aggctccaat cccaccccaa aagtcttgaa tatcgttaat
      721 gtcggggatg ggaccctgaa ctggaccgca tcatggagct catcctggtt aaccgtttcg
      781 ccgtcaagcg ggacagcgcc ctcaaatccg tcggtcagta ttaatatttt tggtatgacg
      841 gcgggcactt atgaggatac cattacgatt gccgatccca atgcggtcaa ttctcctcaa
      901 tacgcgccgg ttacacttca attagtaaat ccccctccgg agatacagct ggttccggac
      961 aatttctctt tcatagctgt gcaggacggc gccaatccgc ccaaccagca gatgacaatc
     1021 aacgatgtcg ggggaggcac gctttcgtgg acagcctcga attccagcac atggctcacg
     1081 ctctccagct acgcaggcgg gcccggagag actatcgatt tgattataga tataaccggg
     1141 cttacttacg gcctctacta tgatacgatt gtggtaagcg atccggaagc atccaactcg
     1201 ccccaactgg cagaggtggt gctcgagctg gtaagttcat tcccggttct tgaggttctg
     1261 ccggattcgt tcgtggttgc gggatcggtc agcgaggatc catacgatca tgtattgaaa
     1321 gtcatcaacg gcggcggggg tacaatgaac tttgaactga ctgccgatca gccctggatc
     1381 agcttcaatc cgcagacagg ttctacaact gatactgccg aggttctggt gaccttccat
     1441 tctgccgggc tgccgttagg atttcagtat gctaccataa cggtttcctc tgacaatgcc
     1501 tctgaatcgc ctcaagaagt aggtgtgcag ttgtgggtca tggaggatcc gccggccctg
     1561 ggagtttcta ccaacactct cagttttagc ggatttccgt gccagaacat cccgcctatc
     1621 gcgggtcaat catttgagat caccaatccc agcccgaatg atctggtctg gtcagcagaa
     1681 tgggatgcgt catggttaag cctttctcct gcttctggtt atagcgagac aaccgttaat
     1741 gtttttgtgg atgaattcgg tctggaaccc ggcacctatg tggatacggt taagattgat
     1801 gctgtctggt ccagtactcc ggacgagtat atcgaggtat cattcacatt ggttgaaaac
     1861 cccagcccgc cggaattggt tttgagtact gattttgttg agttcatatt cctggcaggc
     1921 tatgtggggg cttcctcggc cagtaaattg tatattgata atgcgataag cggatgcatg
     1981 gactggtata taacagaccc ttacccgtgg ctcaatttcg acaagaattc aggtgtagct
     2041 ccggacagcg taatcagtgc ggtcaatggc ggtgggctgc cgctgggcat cacgccggga
     2101 caatttacag ttcacgctcc gggttcatcc aacgattcaa ttgttgtgaa tttcaatgtc
     2161 tacatcgcgc agctgggaga tgccaactgt aacggccaga ttaatatgtc cgatgtggta
     2221 tacatagtaa attatatttt cgtgagcggt cccacgccta ttcccagaat gtgggcggga
     2281 gacgtgaact gcgatttcag ggccaacatc gaagatgcgg tattgctcgt ctcgtacata
     2341 ttcggtctcg ggctggatat ttgccagtac aatccgatag ttaatccgaa tcctgacaag
     2401 taaataacct tctatagaac ttcttgtttc ggcggtgaca gggcataacc ggtcaccgcc
     2461 ttttttaatt gccggcacat ttcataacac taaagcaaag tagtaattcc cccgaactta
     2521 aataatggaa acaagtataa ctttagcaat gttatagggt tagaaaaacg cgatctgatg
     2581 ctggtcaaga gagaatcaaa ttgacttttg gggcttttca ggtatattgt atctgatccg
     2641 gcggcaattg aacagagcca tttgattccg cccgtactgg ctaagaatcc gccataaagg
     2701 tcttttttta aatctatgaa tactcagctt tttttgtgaa tgaatggagg tccctgtgaa
     2761 atcaatttac atcctggcta taattgcatc attgcttctt gggatttcga ctgcttccgc
     2821 tgatgggccg gaggaagctc gggctttttt tgaaaaaata aatgataatg atttgatcat
     2881 cgaggatatc tccaggctca cacctgaaga attggatgag atgattgaaa ctactcatga
     2941 tttctgccgt tacggcaaag cgctgcgcct gcaggcttat cgggatatgg ccgatcaggg
     3001 gttgatcaaa agtgcaactg cagctcagaa cgattgggat gctttatatt atgaactgaa
     3061 ttttggaatc gatttcggta cagaaattat agatggctat gttggccttc aggcggcaag
     3121 cctggtgccc tcgctttctt tcatcgaact tgattttctt gataacatga tcgttgattc
     3181 tgtcatcaag gatggctccc cgcttacttt ttcacatgcc gacaacctga taacaattac
     3241 tctggacaac acctacgaca gctccgagac atttaattta aaggtttatt ataacgggca
     3301 tcctaccgag ggcggcttcc aggccttcga atttgggtac catggtgcaa ctccgatagt
     3361 ctcaactctt tccgaaccat attttgcgcg ttcatggtgg ccatgcaagg attatcccca
     3421 tgataaagcc gattcggtgg atataatcat cactcatccc gatgaatata tctgtgcctc
     3481 caacggtctg gtacagtcga ttgttgataa tggcgatggt accaagacga cgcactggaa
     3541 gcacaattat cctatcacca cctatctggt cgccatcggc gttaccaatt atatagaatt
     3601 tggtgactgg tatgtttccg aaccgggcga cagcatgccg gtcaatttct atgtctaccc
     3661 ggaaaattat tcgcaggcgg tttcatcttt cccgatcact gttgatatga tcgatacact
     3721 ttcggcgata ttcggagagt atccctttat tgacgagaaa tatggaatgt tgcattttgg
     3781 ctggggcggc gccatggagc atcaaaccaa tacttccata agttccagcg cctattatca
     3841 gagcatcata gtccatgaac tcgctcatca atggtgggga gatatgatca catgcgagaa
     3901 ctggcatgag atctggatga atgagggttt tgcaacttat tccgaggctc tctggttcga
     3961 ggctacccag ggtgattcat tttatcatgt ctatatggac gacatggctt atacagcggg
     4021 tggtacaatc tggtgtcagg acaccacctc ggtctggtcg atattcacct cgcgtgttta
     4081 cgacaagggc gcctgggtcc tgcatatgct gcggaactgg gtgggcgaca gcactttctt
     4141 tgaaattctg acgacctatt atgatgatcc cagatacaaa tggaagacca taaataccga
     4201 gcagtttcgt gatctctgta tcgaggtctc cggggactcc cgcctgacgg agttttttga
     4261 ggactggatt tttggcgagt attatcccca gtacaggttt tcgtatattt ttgaggaata
     4321 tcagcccggg gattacatcg tttatctcca tctgcgccag acccaggcca ccaatcccca
     4381 ggtttttgac atgcccatcg agttgagttt ctttgacggt actaactatc atgagaccac
     4441 gatttacaac gaccagaggg agcaggactt catagtttat ctggatgatc tggcgaatga
     4501 cccatatctt ttatggctgg acccggacaa ctggatcctg aagaactccg ccatggaggc
     4561 ctatggcctg aatttaattg attctcctct cgataccgcc taccagtatc aggactatca
     4621 ggattctgta atagcgcggg gcggcacatc tccgcttact tactaccagg tgggcgggag
     4681 ccttcccagc ggtgtggccc tgtacccgaa tggaaaagtg cttggcactc cgctttcata
     4741 tgggagtttc gatttcaagg tcagggtcga ggattccggt tatccgacca aagtcgatac
     4801 gctgactcag agtttaaccg ttctcgagtt tgtgcccctt gttggtgatg ccaatttcga
     4861 cggcctggtt aacgtttccg atgccgtgca cattatcaat tatgttttta tcggcggtga
     4921 tccgcctcag ccgattttgg aagtggggga tgtaaactgc gattccaccg tgaatatctc
     4981 tgacgcggtc tggattataa attacgtctt cgttggcggg cctgcgccgg gggattgctg
     5041 atttcggtat tatgacgaat cgatatttaa ggggtcttca aaggcccctt tgcttttgta
     5101 gttttcccgc atttccgggc accaaaagag caatttttga gttgcccacc aaataaaaca
     5161 tcatatcttg aacgtatgtt tttcggcaat ttaattctgc tgatcgggat tgtatttttg
     5221 ctcagaaacc tgggcgtgat tcacggtaat ttctggggct ggtttatatc atttgctttg
     5281 attctgattg gtataagcat gattttaaaa cagtttagaa aacagaaata aaaatataac
//
LOCUS       DSEB01000197            3362 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003192, whole genome shotgun sequence.
ACCESSION   DSEB01000197 DSEB01000000
VERSION     DSEB01000197.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003192"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            30..299
                     /locus_tag="ENO07_03635"
     CDS             30..299
                     /locus_tag="ENO07_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01089.1"
                     /translation="MNMIRRNHMMVWIDSIEWGGRFNWNENLDSCEFMPDSALHYGTD
                     YMIMFNENGIMGHDGRGMHMGHEDEGYHYYHFHTESRPALSPDSW"
     gene            417..956
                     /locus_tag="ENO07_03640"
     CDS             417..956
                     /locus_tag="ENO07_03640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HDS01090.1"
                     /translation="MKVMVHLVLTLIFMGISNNVFSREDPDRPAEKIEEAEEQAERVE
                     ISTDAEKPFQVYSRNSGNGEFEQVSPEELAEKLAEKAAKENEDFQILLPQNYPNPFNS
                     STVIKYSLPVEAYVYIAVYNIFGQKVRIIKDEIDFPGDYEVTWNGRNEDGASLSSGVY
                     FYIFAADNYYTIGKMIHLK"
     gene            complement(978..2330)
                     /locus_tag="ENO07_03645"
     CDS             complement(978..2330)
                     /locus_tag="ENO07_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDS01091.1"
                     /translation="MNNERILVVDDDASLRRIIEFHLKKKGYKLTALETAEEAVKHLK
                     KSNFELLISDIKMSGMDGLDLLQIAQEIKPDLPVIFITAFGTIEMAVEAMKLGAYDYI
                     TKPFDLDDFMHTVEKALDYHRLLDENLRLKKELKNRDSFSNIIAASKPMEEIFNSVRK
                     VANTDTTVLITGESGTGKELIARAIHRESERSDNPMITVNCAAIPRDLLESELFGHVA
                     GAFTGAVKNKTGKFQLADGGTIFLDEIGDLSMELQAKLLRVLENRVVEPVGSNESTPV
                     DIRVLAATNQDLNKKLKAGEFREDLFYRLNVIPINIPPLRERREDIPLLVKHFLNEYS
                     DSKRLSIDPKALKELSEYDWPGNVRELKNLLKRIAVLAENSKIMPSDLPPEIKSEGSR
                     KTGEDRAGNNSLQETEKNAIVDALRKTGWNQTRAAKRLQIPRHILIYRMKKYGIREEE
                     "
     gene            complement(2332..>3362)
                     /locus_tag="ENO07_03650"
     CDS             complement(2332..>3362)
                     /locus_tag="ENO07_03650"
                     /inference="COORDINATES: protein motif:HMM:PF02518.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HDS01092.1"
                     /translation="VLLIVITAATLTLHYYGLLFEGVLGHSHLIHVIHARLCYIPIVL
                     GAVWFGLRGGITTAVVISIFAVLYIQLKPVIIAEDLINEYTEIAFYLAIGGMAGILVD
                     RDRSFVRKKEEAERKLQQAQRLSMMGQMTASIAHEIKNPLGSIKGASQILKDDSLSDE
                     EKHEFAEMIEKEADRLDFVVKDFLSYARPSPSRFSEIDLREILNSAYKQLKYQARDSN
                     IEINMDFDEVPSIKADPDKLRQVFLNILLNSFQAMPDGGKIEIKCLHDNGDAVIIIRD
                     NGIGIPVQDLDKIYDPFYTTKSRGSGLGLATTKAIIEEHNGAIKADSARGEGTTFKIS
                     LPIEGAEH"
BASE COUNT          824 a          820 c          786 g          932 t
ORIGIN      
        1 cacttgatca ctacggtgga atgggacata tgaatatgat ccgcagaaac catatgatgg
       61 tctggattga ttcaattgaa tggggcggaa ggtttaactg gaacgaaaat ctcgatagct
      121 gcgaattcat gcccgattcc gctctgcatt atggaaccga ttacatgatc atgttcaatg
      181 agaatggtat catgggccat gacggcaggg gcatgcatat gggtcatgag gatgaaggtt
      241 atcattatta tcattttcat accgaaagcc gtccagcatt gagccctgat tcatggtagc
      301 ataaagggcc gaattatgta aacggcagga tttttcttaa cagtcatttg ctgttgtttt
      361 tggctcggaa aatcttaaat tataatagat aatcaacacg gcaccaggag ttaataatga
      421 aagtaatggt gcatttagtt ttaactctaa tttttatggg catctccaat aatgtcttct
      481 ccagggagga ccccgatcgc ccggcggaga aaatagaaga ggccgaggag caggccgaga
      541 gagttgagat ttcaactgat gctgaaaaac cgttccaggt ctattcgcga aactccggta
      601 acggcgaatt tgaacaagtc agccccgagg aactggcaga aaaactggcc gagaaagcgg
      661 caaaagaaaa cgaggacttc caaattcttc tgccgcaaaa ttatcccaat cccttcaatt
      721 caagcaccgt gatcaaatac agccttccgg tagaggccta cgtctatatt gcggtctata
      781 atatttttgg ccagaaggtt agaatcatta aagatgagat tgattttccg ggcgattacg
      841 aggttacttg gaacggtcgc aacgaggacg gcgcatctct ctccagcggc gtctacttct
      901 acatttttgc cgccgacaat tactacacta tcgggaaaat gattcatctg aagtaaccgt
      961 agatcggctc aggttttcta ttcttcttct ctgatgccgt acttcttcat gcggtagatc
     1021 aatatatgtc ggggtatctg caggcgtttg gcagcccgcg tctggttcca tcccgtcttc
     1081 cttaaagcat ctactatcgc atttttttca gtttcctgca gggaattgtt accggctctg
     1141 tcttcgccgg ttttccggct gccttctgat tttatctcag gcggaagatc ggaaggcatg
     1201 atcttgctgt tctcagccag cacagcgatt ctcttcaaca ggttttttaa ttcacgcaca
     1261 ttaccgggcc agtcatactc gcttaattcc ttgagagctt tcggatcaat ggatagtctc
     1321 ttactgtcgg agtattcgtt gagaaagtgt ttcaccagga ggggaatatc ttcccggcgc
     1381 tcgcgcaggg gaggaatatt gatcggaatg acattgagac ggtaaaacag atcttccctg
     1441 aattcacccg ctttcagttt tttattaagg tcttgattgg ttgccgccag tacccttatg
     1501 tctaccggcg tggactcatt cgatccaacc ggttcaacca ctctattttc cagtactcgc
     1561 aagagcttgg cctgcagttc catgctaagg tcgccgatct catccaggaa gatcgttccc
     1621 ccgtcagcca attgaaactt accggtcttg ttcttgaccg caccggtaaa tgctccggcg
     1681 acatggccga acagttcgct ctccaaaagg tcccggggaa ttgcggcgca attgaccgtt
     1741 atcatgggat tgtcggaacg ttcgctctcc cggtgtatgg ccctggcgat cagctcctta
     1801 ccggtgcccg actcacccgt tatgagcact gttgtgtcgg tgttggccac cttcctgacg
     1861 ctgttaaaaa tttcctccat cggctttgag gcggcaataa tattggaaaa tgaatccctg
     1921 ttcttgagct ctttcttgag cctgaggttt tcatccagaa gcctgtgata gtccagagct
     1981 ttttccaccg tatgcatgaa atcatccaga tcaaagggct tggtgatata gtcataggct
     2041 cccagcttca tggcctccac cgccatctca atagtcccga aggctgtgat gaaaatcacg
     2101 ggcagatcgg gctttatttc ctgcgctatc tgcagtaaat ccaggccgtc catacccgac
     2161 attttgatgt cggatatcag aagctcgaag ttggattttt tgagatgctt aacggcctct
     2221 tccgcagttt ccagggcagt tagtttgtag cctttttttt tcaggtggaa ctcgataatc
     2281 ctcctgagcg aggcatcatc atccacaacc agtattctct cattattcat attaatgctc
     2341 cgcaccttca attggcagcg atattttgaa tgttgtgccc tcacccctgg cgctgtcggc
     2401 tttgatggcg ccgttgtgct cttcgataat tgcttttgta gttgccagtc ccaggcctga
     2461 accgcggctc ttggtagtgt agaacggatc gtatattttg tccagatcct gcacggggat
     2521 accgatacca ttatctctga tgattatcac ggcatcaccg ttatcgtgaa ggcactttat
     2581 ctcgattttt cctccatcag gcatagcctg aaatgagttg agcaatatgt tgagaaatac
     2641 ctggcgcagc ttgtcggggt cggccttgat cgaggggacc tcgtcaaaat ccatatttat
     2701 ttcgatatta ctgtcacgag cctgatattt aagctgttta taggccgaat tcagaatttc
     2761 gcgcaggtct atttcagaaa acctggatgg ggatgggcgg gcataggaca gaaaatcctt
     2821 gaccacaaaa tcgagcctgt cggcttcctt ttctatcatt tccgcaaact catgcttctc
     2881 ttcgtccgaa agcgaatcat ccttcaaaat ctgcgaggcg cccttgatcg acccaagcgg
     2941 attcttgatc tcatgcgcaa tggaagcggt catctggccc atcatggaga gcctttgtgc
     3001 ctgctggagc tttctctcag cctcttcctt cttgcgcaca aatgaacgat ccctgtcgac
     3061 caaaattccg gccatgccgc cgatcgccag ataaaatgcg atctcagtgt attcatttat
     3121 cagatcttca gcaatgatta ccggcttgag ctggatgtac agaaccgcga aaatcgaaat
     3181 aacaacagcg gtagtaatgc ccccgcgcag tccgaaccaa accgcgccaa gaacaatcgg
     3241 tatgtagcac agacgcgcat ggatgacatg gatcagatgc gaatgtccca ggacaccttc
     3301 gaacagaagg ccataataat gcagcgtaag tgtcgctgcc gtgatgacga tcagaagtac
     3361 ca
//
LOCUS       DSEB01000198            1530 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1018290, whole genome shotgun sequence.
ACCESSION   DSEB01000198 DSEB01000000
VERSION     DSEB01000198.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1018290"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            16..396
                     /locus_tag="ENO07_03655"
     CDS             16..396
                     /locus_tag="ENO07_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01093.1"
                     /translation="MRNTFIEAGPDNPEEIIRGTRRGLYAADVGFGGQVDVVTGRFIT
                     SIILGYMIEDGKLTYPVKGATITGIGIETLKDIDMVGNDLVMDPSSGRCGKGQEVPVG
                     VGMPTLRVKKLVVGGAGQAFEGQG"
     gene            396..>1530
                     /locus_tag="ENO07_03660"
     CDS             396..>1530
                     /locus_tag="ENO07_03660"
                     /inference="COORDINATES: protein motif:HMM:PF01523.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDS01094.1"
                     /translation="MIDESFAQNLIETAINCGAEKAEAYISEKTETEITIAEGKPESV
                     NVKSDQGYGIRVLLDSKLGFYSSNRLEPLLATENIRQLVRATGLHTSDPFNTLPEHLA
                     DQSSDASEIYDPELAQIPLSEKIKTAIEIEKAGRGADSRVAGFVWILYGDVTESYRII
                     SSTGIDVSSSGTTCYGFAYCFANHGEAVQTGRFAKAYGRYSDFNAGEIGRTAADYAVR
                     MLGARDLRGSNMQAVFPPEAGIPIVHALFGMIEADSVQKGKSPFREKIGKQVASDILT
                     LIDDGTLADGLGTRPYDSEGIPTTRTMVIENGLLKNYLYDTYTAKKGKTKSTGNAARS
                     SYHSKPLIFPTNFFIKPTDIDTNEMIGDVEKGILITELSGLHAG"
BASE COUNT          414 a          351 c          436 g          329 t
ORIGIN      
        1 ccgcccatgc cgaggatgag gaatacattt attgaggccg gcccggataa cccggaagag
       61 attattcggg gcacgagaag gggcttatat gcagccgatg tcggtttcgg cgggcaggtc
      121 gatgtcgtaa ccggaagatt tatcaccagc ataattctgg gatatatgat cgaggatgga
      181 aaactgacct atccggtgaa aggcgcaacc atcaccggca taggaatcga gacgctgaaa
      241 gatatcgata tggtgggcaa tgatcttgtg atggacccct catccggcag atgcggcaag
      301 ggccaggagg tgccggttgg agtgggcatg cccacgctgc gtgtgaaaaa gctggtggtg
      361 ggtggagcag gacaggcgtt tgaaggccag ggataatgat cgacgagagc tttgcacaaa
      421 atcttatcga aaccgcaata aactgcgggg cggaaaaggc tgaggcttat atctcggaga
      481 agacggagac cgagataact atcgccgagg gcaaacccga atcggttaat gtcaaatccg
      541 atcagggcta tggaatccgt gtgcttctgg attccaaact cgggttctat tcctccaacc
      601 gactggagcc gttgctggca acagagaata tccgtcaact tgtgagagcg acgggactgc
      661 atacttcgga cccatttaac actcttcctg aacatttggc ggatcaaagc agcgatgcat
      721 ccgagattta tgatcccgag ctggcgcaaa ttcctctttc tgaaaagata aaaaccgcca
      781 ttgaaatcga aaaagccgga cgtggggcag attccagggt agcgggcttc gtatggatat
      841 tatatggtga cgtaaccgag tcgtaccgta taattagctc taccggaata gatgtcagct
      901 catccggtac aacctgctat ggcttcgctt actgctttgc caatcatggg gaggctgttc
      961 aaaccggacg ctttgccaaa gcctatggcc gttacagtga tttcaatgcc ggggagatcg
     1021 gccgtaccgc tgccgattat gcggtgcgca tgctgggagc cagggatctg agaggcagta
     1081 atatgcaggc ggtcttcccg cccgaggcgg gcattccgat agttcatgcc ttgttcggga
     1141 tgatcgaagc ggactcggtg cagaagggca aatcgccctt cagggaaaaa atcggcaaac
     1201 aagtggcctc ggatatcctg acattaatag atgacggcac gctggcagat ggtctgggca
     1261 caaggccata tgacagcgag ggaatcccga ccaccaggac tatggtgatc gaaaacggtc
     1321 ttttgaaaaa ttatctttat gatacctata cggccaaaaa gggaaagacg aaatcaacgg
     1381 gtaacgccgc gcgcagcagt tatcacagta agcccttgat ttttcccacc aatttcttta
     1441 taaagccaac agatatcgat acaaatgaga tgatcggtga cgtagaaaaa ggcatcctga
     1501 taaccgagtt atcggggctc catgcaggta
//
LOCUS       DSEB01000199            2963 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004223, whole genome shotgun sequence.
ACCESSION   DSEB01000199 DSEB01000000
VERSION     DSEB01000199.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004223"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1564)
                     /locus_tag="ENO07_03665"
     CDS             complement(<1..1564)
                     /locus_tag="ENO07_03665"
                     /inference="COORDINATES: protein
                     motif:HMM:PF02518.24,HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HDS01095.1"
                     /translation="MESRKTSYGQELKSGWFLPLRLAIFLIVVGVVLLTQQRQAAIST
                     PFFIYSILTLVFLLSLALKLPSRMPRLTQFLVFLQIISEVIVEASIIKATGNITSPFT
                     ALFLMTIVSAAMSYQLIGTLLTATMASLTYGLVVLTGSEAELPRTLDLEFIKKIYSSN
                     DELFYTLFLYICVFYLVAFVSGYLSQKIRSKERELRRASESLARVQLETDEILKHLHS
                     GLITIDHFGRIIYFNEAAERITGLKENKIKGKNCLEVFADRMPQFSEKILSVLKSSQL
                     ESRCEITICDNEGEEIPIGISTSVLEDEVRGVRGVIGIFQNLTDAKKMEEKMRHADRL
                     AAVGELSASIAHEIRNPLASISGSVEILKQELNPEGENQRLMDLIVKESSRLSNILTD
                     FLVYARVKTPNFGKVEINRLVSDVIEIARNHTSYREKIKLRSESDDTTIYVSGDDEQI
                     RQVLFNLVVNALEAIESDKGSVKIKIEGNSPIYTDQVILSVIDDGGGMTREVKDRIFS
                     PFFSTKKEGTGLG"
     gene            complement(1615..2733)
                     /locus_tag="ENO07_03670"
     CDS             complement(1615..2733)
                     /locus_tag="ENO07_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDS01096.1"
                     /translation="MEKILRQNKIFASSISRKPTNINLKIGTGIKKIEISRFTRQFAT
                     MIGAGLPMVQCLDILSQQMESAEFRRVVADIKESVQSGTTLSEALGRHKKIFDDLFVN
                     MVEAGETGGALDVILVRLANYREKADALARKVKGALVYPIVVVIVAIGVTLAMLKFVV
                     PTFAKMFTDLGGTLPEPTRIVMGVSNFVNDYFLIMIGGAVAFVVGFRIALRNRSARLY
                     WDTLKLKLPGFGNLIRKTAVARFTRTLGTLLSSGVSILEALEITAKTAGNMVIQNAVR
                     RSMLSIAEGETITQPLKESGVFPPMVVQMISVGEKTGGLDDMLAKIADFYDEEVDAAV
                     AALTSLIEPIVIVFIGVVIGGIMIAMYLPMFDIIGQIK"
BASE COUNT          694 a          810 c          705 g          754 t
ORIGIN      
        1 ggcctaagcc ggtgccctct tttttggttg agaaaaacgg actgaaaatc ctgtccttga
       61 cttcgcgggt catgcctccg ccatcgtcaa ttaccgacag aatcacctga tctgtataga
      121 tcggggaatt accctcgatt tttattttca cgctgccttt gtcgctttcg atcgcttcca
      181 gggcgttcac caccaggttg aacagaacct gcctgatctg ttcgtcatcg ccggaaacat
      241 aaatagtggt atcatcggat tcgcttctga gtttgatttt ttccctgtat gatgtatggt
      301 tgcgggcgat ctcgattaca tcgctcacga gccggttgat ttccaccttg ccgaaattgg
      361 gcgttttgac acgggcatat accagaaaat cggtcaggat attgctcagg cggctggatt
      421 ctttgacgat caaatccatc aacctctggt tctccccttc cgggttcagc tcctgtttca
      481 ggatctccac cgatcccgat atcgacgcca ggggatttcg gatctcatgc gcgatggagg
      541 cggaaagctc gcctactgcg gccaaacggt cggcatggcg catcttctcc tccatcttct
      601 tggcgtcggt aagattctgg aaaattccta tgaccccgcg taccccgcgc acctcatcct
      661 cgagtaccga ggttgagatt ccgatcggga tttcctcccc ctcattgtcg catatggtga
      721 tctcacagcg gctttcaagc tgggatgatt tcaatacaga caatatcttt tcggaaaact
      781 ggggcattct atcggcgaag acttccagac aattcttacc ctttatcttg ttttccttca
      841 acccggtgat cctctccgcg gcctcgttaa aataaataat gcgcccaaaa tgatcgattg
      901 taatcaagcc ggaatgaaga tgcttcagga tctcatctgt ctccagttgc accctggcca
      961 gcgattccga ggcccgacgc agttcccgtt ccttgctcct tattttctgg gagagatagc
     1021 cggagacaaa agcgacaaga tagaaaacgc agatgtacag aaacagggtg taaaaaagtt
     1081 catcgttgga actgtatatc ttcttgataa attcgaggtc cagagttctc ggtagctccg
     1141 cctctgaacc ggtcaatacc accaatccat aagtaagcga ggccatggtg gcggtgagca
     1201 gtgtgccgat tagctggtag ctcatggccg ctgagacaat cgtcatcagg aacagggcgg
     1261 tgaaggggct ggtgatattc ccggtggcct ttatgatcga agcctccact atgacttcgg
     1321 aaatgatctg cagaaatacc aggaactgtg tcaggcgggg catcctggag ggcagtttca
     1381 gcgccaggga aagcaaaaag accaatgtca ggatgctgta gataaaaaaa ggtgtggata
     1441 tagccgcctg gcgctgctgg gtcagaagaa ctacgcctac aactattaaa aatatggcca
     1501 ggcgcagcgg cagaaaccag ccgctcttca attcctgacc ataagatgtt ttcctgcttt
     1561 ccatgacaac aaatatgggc gtccccttgc aggaacgccc atcaacattc tcaattattt
     1621 gatctggccg atgatgtcga acatcggcag atacatggca atcataatac cgccgataac
     1681 gacaccgatg aagacgataa ctatcggctc gatcagggat gtcagggcgg caacagcagc
     1741 atctacttct tcatcgtaga agtctgctat cttcgccagc atatcatcca gcccgccggt
     1801 tttctcaccc acagaaatca tctggaccac cattggggga aacactcccg actccttcag
     1861 cggctgggtg atggtttcac cttcggcgat cgagagcatt gatctcctga ccgcgttctg
     1921 gattaccata ttacccgctg tcttggccgt gatttccagg gcttccagaa tcgatacgcc
     1981 ggaagacaga agcgtgccga gtgtgcgcgt gaacctggcc accgctgttt tgcggatcag
     2041 gtttccgaag ccaggcagct tcagtttaag tgtgtcccag tacagccgcg ccgatctgtt
     2101 tcgcagcgca atcctgaagc ccacaacgaa ggcgacggct cccccgatca taatgaggaa
     2161 atagtcgttc acgaaattcg aaacgcccat cacaatcctt gtcggctcgg gcagcgtccc
     2221 gcccagatcc gtgaacatct tggcaaatgt cggcaccaca aacttcaaca ttgcgagggt
     2281 aacgcctatg gccacgatta cgaccactat cgggtacacc agcgcgcctt tgaccttgcg
     2341 tgccagggca tcggcctttt cacggtagtt tgccagacga acgaggataa cgtccagagc
     2401 gccgcctgtc tcaccggcct ccaccatgtt gacaaacagg tcgtcaaaaa tcttcttgtg
     2461 tctgcccagg gcttcggaaa gcgtcgtacc ggactgcacc gattctttaa tatccgcaac
     2521 aactctgcgg aattctgccg attccatctg ctgggagaga atatcgagac actgcaccat
     2581 aggaagtccg gcaccaatca tggtggcgaa ctgccgggta aaccgtgaga tttcgatctt
     2641 cttgattcca gtaccgatct tgagatttat attggtcggt ttgcgtgaaa tcgagctggc
     2701 gaaaatctta ttctgcctca ggatcttttc catctcagct ttgttcttgg ccttgtaagt
     2761 tccgcttacc tggcctcctg aagccgattt ccccttgtat tcaaaagttg gcaaggttac
     2821 ctccttgctt tattattctt tacactctca ccgacgtttc cgtcttgcgt gatattatct
     2881 cgttcaattc atccacattc gaactccgtc caaccgcatc accatgcgta atcttgcgcg
     2941 acagatacag ttctcccagt gac
//
LOCUS       DSEB01000200            4283 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001889, whole genome shotgun sequence.
ACCESSION   DSEB01000200 DSEB01000000
VERSION     DSEB01000200.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001889"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            159..557
                     /locus_tag="ENO07_03675"
     CDS             159..557
                     /locus_tag="ENO07_03675"
                     /inference="COORDINATES: protein motif:HMM:PF07811.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01097.1"
                     /translation="MAFKSRIFRNKKGNSIIEFALVLPILLLILFGITEFGRAIMVTN
                     VLHTASREGARLASVSDLSDTVSIQTRVQEVLSASNVAPKNITMQYDAATKTVRVSVT
                     SDFEVLSLGILGTFAGVIELQGTTIMKYEG"
     gene            647..1516
                     /gene="cpaB"
                     /locus_tag="ENO07_03680"
     CDS             647..1516
                     /gene="cpaB"
                     /locus_tag="ENO07_03680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HDS01098.1"
                     /translation="MRNIGIILVIFLALVCGAAASVMAYYYLKNNPGEKGPELTPVVV
                     ASHDLTFGETLEPQDMRVVMYPATSVPKNAYAEIDSLKGQITKVFLKENEPILNSKLS
                     SIGGGLSLLIEPNMRAASIQVDKVSGVSGFILPGDMVDVILTVDDYGSRRDAMAKTIL
                     QKVEVLAAGEKTEQKGDKVITVQAVTLLVDAKGVQDLALASQEGKLHLALRNRADNEV
                     VPMSPISKTEILKDNAKPKPKPAPKVTKPRVVVKKEEPVKESDSLVIFRGAAKKTEVP
                     AMAEEKVDRESNK"
     gene            1560..2831
                     /locus_tag="ENO07_03685"
     CDS             1560..2831
                     /locus_tag="ENO07_03685"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00263.19,HMM:PF13629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01099.1"
                     /translation="MKYRILKPAFEMVVIAALLLMCVSSLNAQDQLIRVTKGKSTVIK
                     YHEVIKAVSLANEDVADVVSITATALVVIGKEQGETSLIVWGESEKLTSYEIKVDRNT
                     SGQQVVLDVQVAEVNKTALSEYGFDFLFIDDDDRHIAEGTKILGSYAGEVTTPDPTSM
                     SLPASPNISGVIRWLGDNQEISAVIRTMQRKGDLELLANPHLLCLSGEQASFLVGGEI
                     PVPVAQSVSAGGVPTVTIEWKEFGIMLDFVPTVVDTNLVNLRIMPEVSSLDYTNAVTY
                     GGFDIPALRTRKADAIVELNSGQSIVMGGLLSSESFETIKRIPILGHIPILGFFFSHK
                     QTSKMETELLIIVSPRIITSVEDEVIPPLPFEKDEEKAVKPQKKSKEPLQGSSAPESS
                     PAENSSPEKQPVEGPEEMSDSSFENAEHLSE"
     gene            2845..3936
                     /locus_tag="ENO07_03690"
     CDS             2845..3936
                     /locus_tag="ENO07_03690"
                     /inference="COORDINATES: protein motif:HMM:PF13400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01100.1"
                     /translation="MISNINNNRGASMIMVAVAIVMIFAFSVLAIDVSLILLAKTQLQ
                     NAADAAAIAGATGYYLSAGNQEIAAQDAIAVAGMNYAVQDSMQHVIIDEGDVTFPDEN
                     RITVYTNRTKATKDPVRLYFLKILDPLFDNLGEMRARATARVYPIGGTDCLKPWCFPD
                     QWDDADIDSTYDVGEYYDPDITGYKVPDDVGVQITLKLRNANKSPRTGWYYAVDFGAI
                     NTGDPVITGADAYRQWIAECEPFNISVGDYLQIEPGNMVGPTAQGVGDLIALDPTAEW
                     DPVTGTIINSAYPTSPRIIKVCAFDPSLGVQTDSNGRDYLVVVKIIAAFIENHNGSDV
                     TARFIKRASDGEICEDCEDGFLFKSVLVE"
     gene            3955..>4283
                     /locus_tag="ENO07_03695"
     CDS             3955..>4283
                     /locus_tag="ENO07_03695"
                     /inference="COORDINATES: protein motif:HMM:PF00072.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDS01101.1"
                     /translation="MNEQLTIIMVQADDSLHNVIKDHIDEIEEIRLISEIGDIQTCYE
                     NMESLRPDIILIDLHRSGDVENIFAISSKISIEYPDTAIFISSTIQEPELILTALRSG
                     AQEFFGKP"
BASE COUNT         1146 a          994 c         1161 g          982 t
ORIGIN      
        1 gaatatggct tatcgtctgt gctattttta tatcccgatg cttcgtactg ccggccgagc
       61 agatgaaaaa aagcatccat ataccttatg gcatagcgat aggactggga acattttgtt
      121 acctgttggc gggcaaaatc gtttaagagg ataatgttat ggctttcaaa tccaggatat
      181 tcaggaataa aaaaggcaac tctatcatag agttcgcatt ggttctgccc attttgctct
      241 tgatcctgtt cggaatcacc gaattcggca gggctattat ggtaaccaat gttctgcata
      301 cagcctcgcg ggagggcgcc cgcctggcct ctgtttcaga tttgagcgat accgtttcaa
      361 ttcaaaccag ggtgcaggag gttctctccg catccaacgt ggcgcccaaa aatataacca
      421 tgcagtacga cgccgccaca aaaaccgtga gggtatcggt cacttctgat ttcgaagtat
      481 tgagtttggg catacttggt acatttgcgg gggtaataga actgcaagga acgacgataa
      541 tgaaatatga gggctgatcg aatttcagat gcagtaattg aaatagagtt ctaatgatga
      601 aatatgacgg gttcaataag atgagatttt tggaaggata ttcaagatga gaaacatcgg
      661 aataattttg gtgatatttt tggcgctcgt atgcggcgcc gcggccagtg tcatggccta
      721 ttattacctg aagaacaatc ccggcgaaaa gggaccggag ctgacgccgg tggtggttgc
      781 cagccatgat ctgacattcg gggaaacact tgagccgcag gacatgaggg tggtgatgta
      841 cccggccacg agcgtcccca aaaacgccta tgcggagatt gacagcctga aaggccagat
      901 aaccaaggtc ttcctgaagg aaaatgagcc tatcctgaat tccaaacttt cctccatcgg
      961 aggcgggctg tcgcttttga ttgagccgaa tatgcgcgcc gcctcgatcc aggtggacaa
     1021 ggtatccggg gtctcaggct ttattctccc gggtgatatg gtcgatgtaa ttctgacagt
     1081 agatgattac ggcagccgca gagacgctat ggccaagact atcctgcaga aggtagaagt
     1141 gctggcggca ggcgaaaaga ccgagcagaa aggcgataag gtcattaccg tacaggctgt
     1201 tacattgctg gttgatgcca aaggcgtgca ggatctggcc ctggcatccc aggagggaaa
     1261 actccatctg gctctgagaa atcgtgctga caatgaagtt gttccgatga gtccgatcag
     1321 caagaccgag attctgaaag ataatgccaa gcccaagccc aagcccgctc ccaaagtgac
     1381 caagcccaga gtggtggtga aaaaggagga gccggttaag gaatccgatt cgcttgtgat
     1441 cttcagagga gctgcgaaga aaaccgaggt tccggcgatg gctgaagaaa aggtagacag
     1501 ggaatccaat aaataatcga tagattgacc gatggcgatt tggggaaagg agatccgaat
     1561 tgaaatatag aatcctaaaa ccggcttttg aaatggtggt aattgcggcc ctgctgctga
     1621 tgtgcgtctc aagtcttaac gcacaggatc agctgataag ggtcacaaaa ggcaagtcca
     1681 ccgtgatcaa atatcacgaa gtaatcaagg ctgtttcact tgctaatgaa gatgttgccg
     1741 atgtggtctc gataaccgcc accgcactgg tggtcatcgg caaggagcag ggcgaaacct
     1801 cgctgatagt ctggggtgaa tcagaaaaac tgacatccta cgaaattaaa gtggaccgca
     1861 atacaagcgg ccagcaggtg gtcctggatg tgcaggtggc cgaggtgaat aaaacggccc
     1921 tttcggagta tggttttgac ttcttattta ttgatgatga cgacaggcat atagcagagg
     1981 gtacaaaaat tttgggctcc tatgccggcg aagttaccac tcccgatccg acaagcatga
     2041 gcctgcccgc cagcccgaat atctccggtg tgatcagatg gctgggagac aaccaggaaa
     2101 tatccgcagt cattcggacc atgcagagaa agggcgacct cgagctgctg gccaatccgc
     2161 atctgctctg cctgagcggg gaacaggcca gtttcctcgt gggtggcgag atcccggtcc
     2221 cggtggcaca atcggtgtcg gccggaggcg tgcccactgt gacgatagaa tggaaagaat
     2281 tcggtatcat gctcgacttt gtccccaccg ttgtggacac aaacctggtc aacctgagaa
     2341 taatgccgga ggtctccagc ctcgactaca ccaatgcggt cacatacggg ggctttgata
     2401 ttccggcgct gagaacgcgc aaagccgatg caattgtgga attgaattcg ggtcagtcca
     2461 tagtcatggg cggccttttg tcgagcgagt ctttcgagac aatcaagaga attccgatcc
     2521 tcggacatat tcccattctc ggtttcttct tcagtcataa acagacctcg aagatggaaa
     2581 cggaactgct gattatcgtt tctcccagga tcatcacctc tgtggaagac gaggtgattc
     2641 cgcctctccc gtttgagaaa gacgaggaga aagcggttaa gcctcaaaag aagagtaagg
     2701 agcctcttca gggaagttca gctccggaaa gctcgcctgc ggaaaactca tcccctgaaa
     2761 agcagcctgt tgagggacct gaggaaatgt ccgattcatc ctttgaaaat gcggagcatc
     2821 taagcgagtg aggtggcgat catcatgatc tcgaatataa acaataatag aggcgcaagc
     2881 atgataatgg tggcagttgc cattgtcatg atcttcgctt tctcggtact ggcaatagac
     2941 gtgtccctga tcctgctggc caagacgcag cttcaaaatg ccgcggatgc cgccgcaata
     3001 gccggggcga ccggctatta cctgagcgcg ggcaatcagg agatcgccgc ccaggatgcg
     3061 atagcggtgg ccggtatgaa ctatgctgtt caggattcaa tgcagcatgt aatcattgac
     3121 gagggcgatg taaccttccc ggatgaaaac aggattactg tctacaccaa tcgaacaaag
     3181 gcgaccaaag atccggtcag gctctatttt ttgaagattc tggatcccct gttcgacaac
     3241 ctgggcgaaa tgcgggcccg agccacagcc cgggtctacc caattggcgg tactgattgc
     3301 cttaagccct ggtgctttcc cgaccagtgg gatgatgccg acatagatag tacctatgat
     3361 gtgggcgaat actacgaccc tgatataacc ggctacaagg ttcccgatga tgtcggtgtc
     3421 cagataactt tgaagttgag aaacgcaaat aagtctccca ggacaggatg gtattatgcc
     3481 gttgatttcg gtgccatcaa caccggcgat cccgtgataa caggagcgga tgcctacagg
     3541 cagtggattg cagagtgcga gccgttcaat atctcagttg gcgattatct ccagatcgag
     3601 cccggtaata tggtgggacc gacagcccag ggagttgggg atctgattgc cctggatccc
     3661 accgccgagt gggacccggt aacaggaacg ataataaact cagcttatcc gaccagtccc
     3721 cggataatta aagtttgtgc ctttgacccc agcctgggag ttcagactga ctccaatggc
     3781 agggactatc tggttgttgt caaaattatc gcggctttca tcgaaaacca taacggcagt
     3841 gatgttaccg cccgctttat taagcgagca agtgatgggg aaatatgcga ggactgcgag
     3901 gacggatttt tattcaagtc ggttctggtg gagtaaaaat atcggaggac aaaagtgaat
     3961 gaacagttga caataataat ggtacaggcg gatgattcct tgcacaatgt gatcaaggac
     4021 cacattgacg agatcgagga aatcaggctc atatccgaga tcggcgatat ccagacgtgc
     4081 tatgagaata tggaatctct caggccggat ataatactga tcgatcttca caggtccggt
     4141 gatgttgaga atatctttgc catctccagc aaaatcagca tcgaatatcc ggataccgcc
     4201 atctttatca gctccacaat ccaggagcct gaattgatac tgaccgccct gcgttccggt
     4261 gcccaggaat ttttcggcaa acc
//
LOCUS       DSEB01000201            3158 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003692, whole genome shotgun sequence.
ACCESSION   DSEB01000201 DSEB01000000
VERSION     DSEB01000201.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003692"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            141..213
                     /locus_tag="ENO07_03700"
     tRNA            141..213
                     /locus_tag="ENO07_03700"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:174..176,aa:Phe,seq:gaa)
     gene            420..1250
                     /locus_tag="ENO07_03705"
     CDS             420..1250
                     /locus_tag="ENO07_03705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01102.1"
                     /translation="MRKKLCLIAVTTIAVFFALNLTDSNAEYICGDANDDGSLNVSDA
                     IYIANYVFVGGPVPDPNCCESDCPPTVTDIDGNTYFTVEVGDQCWMAQNLKVTHYSNG
                     DPIPNVTDGGVWGGLTTGAYCDYNNEAGNAAIYGRLYNWYAVIDIRDIAPEGWHVPTD
                     AEWKQLEIFLGLSLAEADAEGWRGTDEGGKLKEIGIMHWGSPNTGATNESGFTVLPAG
                     TRSVSGNFSNLGADARLWSSTEASSTGGGYRWLEYSHSDIYRFRYVKGGGLSVRCVKD
                     "
     gene            1462..2409
                     /locus_tag="ENO07_03710"
     CDS             1462..2409
                     /locus_tag="ENO07_03710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01103.1"
                     /translation="MSKNLYLFVFVGVAAMICLAATAANSEYVCGDANDDGTLNVSDA
                     VFIITHVFIGGPAPDPWESGEVNCDGALNISDAVRLINYVFKAGYAPCDTDGDGIPDC
                     GFYCPPTVTDYDGNVYHTVLIGDQCWMMDNLKVTHYSNGDPIPNVTDSAEWEGLTTGA
                     YCNYNNDEGNVAIYGRLYNWYAVDDSRNIAPVGWHVPSDAELKQLEMYLGMSRAEADA
                     TGLRGTDEGGKLKEAGTTHWYAPDTGGTNESGFSALPGGCRYIGGTFGDMGNFAYFWS
                     SPESSSNRAWLRLLYYGSSYVFRFSGDKRYGFSVRCVRD"
BASE COUNT          823 a          648 c          816 g          871 t
ORIGIN      
        1 ccttaaaagc ctgtttatga gcaggaggag aagggcgcat tttactgaaa taaggtcatt
       61 gtttagatgg tcgaaaaagg gccagaatgg taaaaagata ttgacttggg tcgaaaaaaa
      121 ttgtatagag ttgattcact ggcgccctag ctcagttggt agagcaacgg actgaaaatc
      181 cgtgtgtccc cagttcgatt ctggggggcg ccatattttt atgccaacag ctaagacctg
      241 agctgtatcc acagcatcat ttgcccctct ctgaaaaaaa tgacaaaaag gttgactttg
      301 gttcatgtgt acatatgtat ggattcagcg gacaagcact aaaatgaaac cgacaccctc
      361 agagttcgca atatgtgagc attcaagatt tttaacattt ttcttggagg gatattccta
      421 tgcggaagaa attatgtttg attgctgtaa caaccatcgc tgtatttttt gcactgaacc
      481 ttactgattc aaatgcagaa tacatctgtg gtgatgccaa cgacgatgga tcgctaaacg
      541 tcagcgatgc aatttacatt gctaactatg tcttcgttgg cggtccagtt ccagatccga
      601 attgttgtga atctgattgc ccgccgactg tcacagatat agatggcaac acatatttta
      661 cggtagaggt cggcgatcag tgctggatgg cccagaattt gaaagtgacc cattatagta
      721 atggcgaccc gattccaaac gtgaccgacg gtggtgtatg gggaggactc acaacggggg
      781 cctattgtga ctataataac gaggcaggca atgctgccat ctatggaaga ctctataatt
      841 ggtatgcagt gattgacatc cgtgatatag cgccagaagg ctggcatgtg ccgactgatg
      901 ccgaatggaa acagctggag atttttctcg gccttagtct ggcagaagcc gatgccgaag
      961 gctggcgagg tactgatgaa ggggggaaat tgaaggaaat cggtattatg cattggggct
     1021 ctcctaacac gggagctacg aatgagagtg gttttaccgt gcttcccgcc ggtactcgat
     1081 cggtcagtgg taacttcagt aatttagggg cagacgctcg cttatggtcc tccactgaag
     1141 ccagtagtac cggcggcggg tatcgatggc tcgaatacag tcattcagat atttaccgct
     1201 tcaggtatgt caagggaggc gggctgtcgg ttcgatgtgt caaggattga tgggtcgtct
     1261 tagaagaacc atagtggctg aaaaatagcg aatagtttag tcccgggcag ggtccgtgat
     1321 caaatccgga tccgggtgaa aatccaaaac atcgattaaa atgttgaata tttgagcttt
     1381 atcgctatat tataagtgcc aacctgaatg atttatcgca gatgaaatcg cccctatgtc
     1441 tgccaagaat aggatagtgg tatgtcaaag aacctatatc tgttcgtatt tgtaggcgtg
     1501 gctgcgatga tctgccttgc cgcaactgcc gccaatagcg agtatgtctg tggtgatgcc
     1561 aacgacgatg gcacgctcaa tgtcagcgat gcagttttta ttatcactca tgtttttatc
     1621 ggtgggccgg cacccgaccc ctgggaatcg ggtgaggtta actgtgatgg cgctttgaat
     1681 atctctgatg ctgtccggct gataaattac gttttcaaag caggctatgc cccgtgcgat
     1741 accgacggcg atggaattcc agactgtggc ttttattgcc cgcctactgt taccgattat
     1801 gatggcaatg tttaccatac agtgctaata ggagatcaat gctggatgat ggataatctg
     1861 aaggttacac attatagcaa tggcgatcct ataccaaatg taacagatag cgcggaatgg
     1921 gaaggtctga ctacaggagc atattgcaac tataataacg atgagggtaa tgttgccatt
     1981 tatggcaggt tatataactg gtatgcggta gatgacagcc gcaacatagc ccctgtaggc
     2041 tggcatgtcc ccagtgatgc ggagttgaaa caattggaga tgtatcttgg tatgagccgg
     2101 gctgaggccg atgccaccgg tttgcgcggg actgatgaag gcggcaaatt aaaagaggca
     2161 ggcacaaccc attggtatgc accagatact ggcggtacta atgaaagtgg cttttccgcc
     2221 ttgcccggtg ggtgccgcta catcggtggt accttcggcg atatgggtaa cttcgcttac
     2281 ttctggtcat ctccggagag cagtagtaac cgcgcatggc tccgactcct gtattacggc
     2341 agttcgtatg tcttccgctt cagcggcgat aagcgctacg gtttttctgt tcgttgtgtc
     2401 cgggattagt tgactatttg actgtttgcc ccgtggggca ggccatgagc cgttgtccct
     2461 gtgttttgtt tggggaaaaa gatcggaagg aatagatcga gtggcaaatt atctcacgcg
     2521 gcaaagggga taggcgcctc tatcaaggca gttttatcca ccgctggaat ttatttcatc
     2581 ccgataatgg caatattttc cgcatctgga aaatctgtac gcatcagaca ccgaatccat
     2641 cccatttata agcgctgaca gggcagaaac ttataagatt gatccggaca tcttttcaat
     2701 gtggcacagc ttttgaaatc aatttgggca gactgaacaa attaaggaga agcaatggga
     2761 aagctcttga ttctgatttt agcgttgatt ttggttctgg tgattctgcc cggagacgtc
     2821 agagactggt gtgttatgca gtttaataca ttgatggccg gagctattga ttccggcaac
     2881 ggtgcctctt tggccaatat ttcagcagta acgtttcaaa tcatttgaga agataaaaaa
     2941 agcttaaatt caatttatat aaattacatg tagcgctgat ggattccagt tattgacaag
     3001 accttaattt ggcgtgcttt tttgatgctt ccctcctttt gtttgaggcg gatccctccc
     3061 gatccgcctt tattattttt cattaccgac agaaaccatt aaatccccag gatgtttaaa
     3121 tcaggacagg aactatatat agccatacgg cttttggc
//
LOCUS       DSEB01000202            2463 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1006401, whole genome shotgun sequence.
ACCESSION   DSEB01000202 DSEB01000000
VERSION     DSEB01000202.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1006401"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..464)
                     /locus_tag="ENO07_03715"
     CDS             complement(<1..464)
                     /locus_tag="ENO07_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01104.1"
                     /translation="MMVISKKNRWRLAVTDPVFFAREFLEIEPHEGQTRWLMNSNKAE
                     NLLHTGNRWGKSMAQAIKILHRCVFKVRNTAYDSCGKYNAVNCSITLDQAKIIFNNVQ
                     RVIKGNPLMEMLVNCVRYTPYPRIYFGNDAVFSCRSTQRRGEYILGQDYDYC"
     gene            complement(461..1768)
                     /locus_tag="ENO07_03720"
     CDS             complement(461..1768)
                     /locus_tag="ENO07_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01105.1"
                     /translation="MGRFKRLINVFRKNSSRIPADFADNKSIERESLAKFTEKRSLKK
                     APDQFGYRIMLPVGDSLQKSRVNLYRFLIDTIPALNAAVWTWTSLSASPIKIEITGTD
                     NGETIEKATKAVEQMFSHAYDNRYQKFAGVDGLLLEYFTSLYSTGSAAGELVGLPGGN
                     GIDYFYFIDPASLGFKMRDGVWRICQQQENKKVWLDQGSTYFYGLKADSVNPGGNSLL
                     RSIPFVARVEQQMINDMHKSMHNAGYHRIHVKVTPPDRLPGESEKNYVSRANNYFEKT
                     ASMFRDFRPEDNPITWDDVGIEYIGPSSRASSTGSWYMNHRAVIEDICAGMHLAPFML
                     GYSYGTTHNWAMFKYELVQREVRSVQQAAAGFLEWMANIELALKGFDVSCHIGFANEV
                     VYGLKDKMDAEQVRIKTIIMKKEAGLLDTAEAKREMKTGRTLS"
BASE COUNT          600 a          643 c          550 g          670 t
ORIGIN      
        1 caacagtaat cataatcctg gcccaggata tactcgcccc gcctttgagt cgaacggcag
       61 ctgaataccg catcattgcc gaagtaaatt cgtggatagg gtgtatagcg cacacagttc
      121 accagcattt ccatcaacgg attgccctta atcaccctct gcacattgtt gaagatgatc
      181 ttcgcctgat ccagagtgat cgagcagttc acggcattgt atttcccgca cgagtcataa
      241 gcggtattcc tgaccttgaa gacacagcga tgcagtatct tgatcgcctg cgccatgctt
      301 ttgccccagc ggttgccggt atgcaggaga ttctcagctt tgttggagtt catcagccat
      361 ctggtctgcc cctcatgcgg ttcgatctcc agaaactccc gcgcgaagaa aaccggatcg
      421 gttaccgcca gacgccacct gttcttcttc gatataacca tcacgaaagt gtccttccag
      481 tcttcatttc acgcttcgct tctgcggtat caagcaggcc agcttccttt ttcatgatga
      541 tagttttgat tcgcacttgc tcggcatcca tcttatcctt caaaccgtaa acgacttcat
      601 tggcaaaacc aatgtggcag ctgacatcaa atccctttaa agccagctcg atattagcca
      661 tccattccag aaagcctgcc gccgcctgct gcaccgacct gacctctcgc tgaaccagtt
      721 cgtatttgaa catggcccag ttgtgagtgg taccgtatga atatcccagc atgaatggcg
      781 ccaggtgcat gcccgcgcag atatcctcta tcaccgcacg atggttcatg taccatgagc
      841 cggtcgagga tgctcttgat gacggcccga tatattcgat cccgacatca tcccatgtaa
      901 tcgggttgtc ctccggacga aaatccctga acatcgaggc ggtcttctca aaataattat
      961 tagcgcggct gacgtaattc ttttccgact cacccggcag acggtcaggc ggcgtgactt
     1021 ttacatgtat tcgatgatac ccggcgttat gcatgctctt gtgcatatcg tttatcatct
     1081 gctgttccac ccgcgccaca aacggtatgc tcctcaggag cgaattgccc ccgggattga
     1141 ctgagtccgc tttaagtccg tagaaatatg tcgagccctg atccagccag accttcttat
     1201 tctcctgctg ctggcagatt cgccagacac catcccgcat cttaaatccc aggctggcgg
     1261 gatcgataaa atagaaataa tcgataccgt tcccgcccgg aagcccgacc agttctcccg
     1321 ccgccgatcc agtggaataa agcgaggtga aatattccag aagcaggcca tcaactccgg
     1381 cgaacttttg atatcggtta tcatacgcat gagaaaacat ctgctcaaca gccttggtcg
     1441 ctttctcgat agtctcgcca ttatcggtgc cggtgatttc aattttaatg ggcgaggccg
     1501 agagcgaggt ccaggtccag actgcggcgt tgagcgcagg aattgtgtcg atcagaaagc
     1561 gatagagatt gacgcgggat ttctgaagcg aatccccgac cgggagcatg atccggtatc
     1621 caaattgatc cggcgccttt ttcaggctgc gtttttccgt aaattttgcg agcgattccc
     1681 tctcaatcga cttattatcc gcaaaatctg caggaattct gctgctattt tttctgaaga
     1741 cattgattaa cctcttgaat ctacccaaat taacctcctg attttacaaa ataatccgga
     1801 aaattatcag gagtgaaatt gtagcaaaac cgcatgaata ctgcattttc cgcttgcatt
     1861 ctgattttga tgtgttattt tactggcgca gaatgacagg gagtgctgtc tgattggccc
     1921 aataaagaag ccaaaagcat ttttattcaa tatttccccg ccttgccaag cagaaagtac
     1981 tccctcttct ttttgccccg catcgcctgc gacccgctaa tccattgtta gttaactaat
     2041 tatggtatag ccccggattc tgcccaagtg atctggcgca taatatgcgt ttgggaattt
     2101 gcgcggagtt ttccacaaaa tgttaaaata ttgtgcggca tatggttacg atttcgcatt
     2161 ttgggtgaaa catttggcac aaagtttgat atatataaag cacggatagt tacttttgga
     2221 gcttactcca aacgggctta agaggtaaga ctctttattg gtagtggctt gagtaacaag
     2281 aaataaggtt cgagaggtta ggagaggttc agtaatttgg gcctctctgc tgagaatcag
     2341 acccaataag ggctttccat ataaactgtt tctcctcctg accggccatt tccccatggc
     2401 cggtcttatt taatctgctt tgaagaaaca gcgttggtca ataaccgtca ggcgccccac
     2461 ctg
//
LOCUS       DSEB01000203            4141 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002024, whole genome shotgun sequence.
ACCESSION   DSEB01000203 DSEB01000000
VERSION     DSEB01000203.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002024"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1328
                     /locus_tag="ENO07_03725"
     CDS             <1..1328
                     /locus_tag="ENO07_03725"
                     /inference="COORDINATES: protein
                     motif:HMM:PF07992.12,HMM:PF14759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HDS01106.1"
                     /translation="LASFEVKIEGDDIYVMIPEEFKGSRTPDMVSYDPGSENRSFVII
                     GAGAAGSMAAQTLREDGFKGQVIMLTHEDRMPYDRPNLSKDYLQGDAKEEWMPIRDDD
                     FYNKYGIEVLRKKHVTGIDIKNREVSVASGDSIKYDRLLITTGGEPRKLDIPGADLQN
                     VFTLRSYDDCDRIIAAAENASKVVVIGASFIGMETAFSLTKRKLKVTVAAPEKTPFSH
                     VFAAEVGELFRKKHEDIGVQFRLGESVTEIEGDGKVKAVVLESGDRIEADMVIMGVGV
                     KPSSEFLKGLELESDGSLKVDEYFNVTEDVFAAGDIASFPYWYNGDIIRIEHWRTAEQ
                     QGRYAAHNMLDKDKPFRSIPFFWTAQVGLQFRHVGYVRDWEDVIVKGDVSKGSFLLFY
                     VKNNRVQAASGIKYDKEIDAVEELMRRDRMPSADELKDGSIDLLELVRK"
     gene            1352..3433
                     /locus_tag="ENO07_03730"
     CDS             1352..3433
                     /locus_tag="ENO07_03730"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDS01107.1"
                     /translation="MHDKNNDKGEHDHKNNNSQDEVVEHKHKDHEGHEENHDGKDHKS
                     HHAHMAEDFKRRFWISLVLTIPIIVLSPMIQGFVGLKDQLAFPGDSYVQFVFASAVFF
                     YGGFPFLKGLVKELREKQPGMMTLIALAIMVAYVYSSAVVFGLAGRTFFWELATLIDV
                     MLLGHWIEMRSVMGASKALDELAKLMPSDAHKLEDDGSTKDVPVSELEKGDKVVIKPG
                     EKVPVDGKITEGSTSIDESLVTGESEPVSKGEGDDVVGGSVNGDGSITVEIEKTGKES
                     YLSQVMDMVESAQASKSKSQNLADKAAMWLTFIALGAGTITLILWLTVFGREFVFALG
                     RMVTVMVITCPHALGLAIPLVIAVSTALSAKNGLLIRNRVPFESARNIDAIVFDKTGT
                     LTKGTFEVSEILAIGDNTDKDEILMYAASVEARSEHPIAKAITEASDDKKKVESFESI
                     KGKGATGKVDGKDVKVVSPGYLDENKIDISEEATEFAKKGKTIAYVLIDDKVKGAIAL
                     EDPVREESKEAIKKLKERNIKTLMITGDNEHVAKRVADEIGLEDFFAGVLPDKKAEKI
                     KEVQSEGYTTAMVGDGVNDAPALAQADVGIAIGAGTDVAMETADIVLVKNNPLDVVKI
                     IAYSRATYKKMVQNLIWATGYNAVAIPLAAGALYSFGILLNPAVGALLMSLSTVIVAI
                     NAKFLKVPEIE"
     gene            3591..>4141
                     /locus_tag="ENO07_03735"
     CDS             3591..>4141
                     /locus_tag="ENO07_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HDS01108.1"
                     /translation="MANQDKLESMRQVEHENREWLESLEYVYQNQGIRRVGELLGHLQ
                     YRAQQMGVEVCFTPNTPYINTIHVRDQAVFPGSREIERRIKSIIRWNAMAMVVRANRK
                     SSGIGGHISTYASAATLFEVGFNHFFRAPSEKHPGDIVYFQGHASPGVYARAYVEGRL
                     DETHLENFRRELQPEVGLSSYPHP"
BASE COUNT         1091 a          898 c         1171 g          981 t
ORIGIN      
        1 ctctggcctc attcgaggtc aagatcgagg gtgatgatat ttatgtcatg attccggagg
       61 aattcaaagg cagccgtacc ccggatatgg tcagctatga tcccgggagc gagaaccgca
      121 gttttgtaat aatcggcgcg ggtgcggcag gttccatggc cgcccagacc ctccgcgaag
      181 acggtttcaa ggggcaggtt atcatgctta cccatgagga tagaatgccc tacgatcgtc
      241 ctaatctcag caaggactac ctgcagggcg acgccaagga agaatggatg ccgatcagag
      301 atgacgattt ttataacaaa tacggaatcg aggttctccg caaaaagcat gtcaccggaa
      361 tcgacataaa aaacagggaa gtctctgttg caagtgggga ctctataaaa tatgaccgcc
      421 tcctgattac caccggtggg gaaccgcgca agctggatat tcccggcgca gacctgcaga
      481 atgtcttcac cctgagatca tatgatgatt gcgatcggat tatagccgcc gccgaaaatg
      541 cttcaaaagt agtggtgatt ggtgccagct tcataggtat ggagacagca ttcagcctga
      601 ccaaacgtaa gctgaaagtc actgtggcgg cgcccgagaa aactcccttc agccatgtct
      661 ttgcagccga agtgggcgag cttttccgca aaaagcatga ggacatcgga gtccaattca
      721 ggcttggaga atcggttacc gaaatcgagg gtgacggtaa agtcaaagca gttgttcttg
      781 aaagcggaga caggatagag gccgacatgg tcatcatggg agtaggtgtt aaaccatcct
      841 ccgagtttct gaaagggctg gagcttgaat cggacggcag tctcaaggtg gacgagtatt
      901 ttaatgttac cgaggatgtc tttgcggccg gtgatatcgc cagttttcca tattggtata
      961 acggcgatat tatacgcatt gaacactggc gcaccgcgga acagcaggga cgctatgcag
     1021 cccataatat gctggataag gataagccgt ttagaagtat acccttcttc tggacagccc
     1081 aggtcggcct gcaattcagg catgtcggtt atgtccgcga ttgggaagat gtcatagtta
     1141 agggtgacgt ttccaagggt agctttctgc ttttctatgt caagaataac agggtgcagg
     1201 cggcctctgg aataaaatac gacaaggaaa ttgatgcggt ggaagaactg atgcgccggg
     1261 atcgcatgcc ctcagcggat gaattgaagg atggttctat tgatttgctt gaactggtaa
     1321 ggaaatagat ctattccgaa aggaatcaac gatgcacgac aaaaataatg acaaagggga
     1381 gcatgatcac aaaaataata attctcagga tgaagtggta gagcacaagc ataaggatca
     1441 cgagggccat gaggagaatc atgatggaaa ggatcataaa agccatcatg cgcacatggc
     1501 tgaggatttc aaacggcgct tctggatctc gctggtcctt acaataccta tcatagtttt
     1561 atcgcccatg attcagggat ttgtgggcct gaaagatcag ctcgcattcc cgggcgattc
     1621 ctatgtacag tttgttttcg catccgctgt cttcttctat ggcggctttc cgtttctcaa
     1681 ggggctggta aaggaactgc gcgaaaaaca gccgggtatg atgaccctga tcgcgctggc
     1741 gattatggtt gcctatgttt atagcagcgc ggtagtgttc ggccttgcgg gaaggacatt
     1801 tttctgggag ctggccaccc tgattgatgt catgcttttg gggcactgga ttgagatgcg
     1861 ttcggttatg ggggcctcta aagctcttga tgagcttgct aaactgatgc cctcggacgc
     1921 acataagctc gaggatgacg gttcgactaa agatgttccg gtcagtgagc tggaaaaagg
     1981 ggataaagtt gtgattaagc cgggcgagaa ggtaccggtt gacggcaaga taacagaggg
     2041 ttcgacgtcg atagacgagt ccctggttac cggtgaatcg gaacctgttt ccaaaggtga
     2101 gggcgatgat gttgtgggcg gttctgtcaa tggtgatggc tccattacgg tcgagataga
     2161 aaaaaccggc aaggaatcat atttgtccca ggtgatggac atggttgaga gcgcgcaggc
     2221 cagcaaatcc aaatcccaga atctggccga caaagccgcg atgtggctga catttatagc
     2281 ccttggcgcc ggtacaatta cactgatttt atggctgact gtgttcggca gggagttcgt
     2341 ttttgccctg gggcgaatgg tgactgtcat ggtgattacc tgcccgcatg ccctcggtct
     2401 ggcgattccg ctcgtgattg cggtctccac cgcgttatcg gccaaaaacg gcctgttgat
     2461 ccggaaccgt gtgcccttcg aatcggcgcg aaatatagat gcgatagtat ttgataaaac
     2521 cggcactctg accaagggaa cattcgaggt ctcagaaatt ctggcaattg gtgataatac
     2581 ggataaagat gaaattctga tgtatgcggc ttcggtagag gcacgttcgg aacatccgat
     2641 tgcaaaagcc attacggagg cgtctgacga caagaaaaag gtggaatcct tcgaatctat
     2701 caaaggcaag ggagcgaccg gaaaggtgga tggcaaagat gtcaaagttg ttagcccggg
     2761 atatctggac gaaaacaaga tcgatatcag tgaagaagcc acagaatttg cgaaaaaggg
     2821 caagaccatc gcctatgtcc tgatcgatga taaagttaaa ggtgctatcg cgcttgagga
     2881 tccggtgcgc gaagaatcca aagaggcgat aaagaaacta aaggaaagaa atataaagac
     2941 attgatgatc accggcgaca acgagcatgt cgccaaaaga gtagcggatg aaattgggct
     3001 tgaggacttt tttgccggcg tgctgcctga taagaaggct gaaaagataa aagaggtgca
     3061 gtcggagggc tatactactg cgatggtggg agacggtgta aatgatgctc cagccctggc
     3121 acaggcggat gttgggattg ctatcggcgc cggaaccgat gtggccatgg aaacggctga
     3181 tattgttctg gtcaagaaca atcccctgga tgtcgtcaaa atcatagcct actcgcgcgc
     3241 cacttacaaa aagatggttc agaatcttat ctgggcaacg ggatataatg cggtcgcaat
     3301 cccgctcgcg gccggggcgc tttacagctt cgggattttg ctgaatccgg cagtgggagc
     3361 tcttttaatg tcgctgagta cagttatagt ggcaattaat gccaaattcc tgaaggtacc
     3421 ggagattgaa tgatactgag atgaaagtat tatagagggg tgctatattg tttctgccgg
     3481 cccgaacata tgtgaattca tattgtttta agtataatat cttttccgaa taatgtttcg
     3541 gactaatttg aacattgaaa atattggagt caggagcgaa aggtgagctt atggccaatc
     3601 aggataaatt agagtcaatg cggcaggtgg aacacgagaa ccgcgaatgg ctcgaatcgc
     3661 tcgagtatgt ttatcaaaac cagggcatcc gccgcgtggg ggagctcctg gggcacctgc
     3721 agtatcgtgc ccagcaaatg ggtgttgaag tctgcttcac tcccaacacc ccctatatca
     3781 acaccattca tgtcagggat caggcggttt ttccgggcag ccgggagata gagcggcgca
     3841 taaagagcat tatccgctgg aacgccatgg ccatggtagt gcgtgccaac cgtaaatcga
     3901 gcggtatcgg gggccatatt tccacctatg cctccgccgc aacgttattt gaagtcggtt
     3961 tcaaccactt tttccgcgca ccctcggaga aacatccggg cgatattgtg tatttccagg
     4021 ggcatgcttc ccccggcgtt tatgcgcgcg cttatgttga agggcgtttg gatgagactc
     4081 atcttgagaa cttccgccgc gagcttcagc cggaggtcgg gttgtcatca tatcctcatc
     4141 c
//
LOCUS       DSEB01000204            3156 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003696, whole genome shotgun sequence.
ACCESSION   DSEB01000204 DSEB01000000
VERSION     DSEB01000204.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003696"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            929..1519
                     /locus_tag="ENO07_03740"
     CDS             929..1519
                     /locus_tag="ENO07_03740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDS01109.1"
                     /translation="MNEKELVKKAQSGDFEAFSRLIEKYKNKIYNLGFKLSGNRHDAE
                     DILQETFLKAVDNIDKFRGESSFGTWLYAIAVNAVNALHQSKKKAELKPIEDFLPGGH
                     GSGDGAKELFEWGDPHELLEQKELNRVIDDTLAEMSDKYSVPFILRYYEDLSVKEVAD
                     SMNLSVAATKSRILRARLALRERLSEVFKEKANERV"
     gene            1506..1754
                     /locus_tag="ENO07_03745"
     CDS             1506..1754
                     /locus_tag="ENO07_03745"
                     /inference="COORDINATES: protein motif:HMM:PF13490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01110.1"
                     /translation="MSECKKHIQSIADYIDGELGPELCSEIEEHLKGCKNCRLMVDTL
                     RQTVILCKDGEREELPESISSKLNEAIKRRWEKKFGKA"
     gene            2135..>3156
                     /locus_tag="ENO07_03750"
     CDS             2135..>3156
                     /locus_tag="ENO07_03750"
                     /inference="COORDINATES: protein motif:HMM:PF03176.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HDS01111.1"
                     /translation="MKKALTEFSINHPWIVIGIVAVITVFFALQLPNIKIDTDPENML
                     EEDEPVRVFDRQVKEEFGISDFIAVGVVSQEGAFNEEILTNVYNIIEEIEDIDGVIAD
                     DIMAPSTVDDIKQTPDGILKVETLLEGEPESDSAARYVLGRIKGNPILRGKLGSDDGK
                     AIAMFIPIESKDMSYRISNEIKAIIDKHSNGEEYYIAGLPIAQDSFGAEMFKQMAISA
                     PMAGAIIFLLMLLFFRQIKVVIPPMIVAVVSVIWAMGLLIATGNTVHIMSSMIPIFLI
                     PIAVLNSIHIISEFHDRYQKHKHKKTTIRNTISELFIPMLFTSLTTVVGFLSLATTPI
                     PPVRVFG"
BASE COUNT          866 a          692 c          796 g          802 t
ORIGIN      
        1 atcggaagga cagcgaccgg gaggagacat tccgggacga agaggatatc gatattttcc
       61 ctgatgatag acagctggtc gcatttgaac gtgtcaccga cccaacccgg tttgagccgt
      121 ttgacctgga ggaaatgata ggggcttcag ctgagcttta ctggcgctac catctaacta
      181 caatggcaag ggcggattac aaggacaagg aatttcaaaa tcaattcgtg gcggaattat
      241 ttaaattccc tgaccccaat acggccttcg ggctttatac tctgattcgt cccgatgact
      301 gcgagattat taagattgga acggaggctt ttcttgattc caactgcata actttactca
      361 aggaccggta tgtcagcgat gtctcctgtc catttcctgt gcccaaaatg gaggacaagc
      421 tgatcaagct ggcacgggcg gtggaagcga aaattcccgg aacggcgcag tacccggaag
      481 agttatctgt ttttccggca gaagacaaga tcccctgttc cgaagcctat attcacaatt
      541 actttttgaa tcagagcttt tttacacgcg ttttcacatg tgactatgat cccggatacg
      601 gcaggattac cgtattctat ctgccccggg atggcttgca ggccctcgat tcgtacctga
      661 gttttatcgg attgaaaggg gacctgagag atagcgctga ggttggcggc tgccagtcgt
      721 atgccgtagt tgacccggag ttcggagaga tttatctcta ttctgccgga gaggacctgc
      781 ttggaatcat caacaaaagc tcccaggaac ttcccctcga attcggtgca aaatatattc
      841 gcggtcttcg cggatacagg acgcattaat tttcaccccc ggattttttt gttttttcct
      901 gaatcattta gcgtatattc caatccaaat gaatgaaaaa gagttggtga aaaaagctca
      961 atcaggcgat ttcgaggcat tcagccggct gattgaaaag tacaaaaaca agatttataa
     1021 tcttggtttt aagctctcgg gaaatcggca cgatgccgag gatattctcc aggagacatt
     1081 cctgaaagcg gttgataaca tcgataagtt tcgcggcgaa tcttccttcg gaacctggct
     1141 ctatgctata gcggtcaatg ccgtgaatgc gcttcaccag agtaaaaaga aagccgagtt
     1201 gaaaccgatc gaggattttc tgccgggcgg gcatggttcg ggagacggag caaaggaatt
     1261 attcgaatgg ggagatccgc acgagctcct tgagcagaaa gagctgaatc gagtgatcga
     1321 tgacactctg gcggaaatgt cggataaata cagtgtgccc ttcattctga gatattatga
     1381 agatctctcg gtcaaggagg tggccgattc gatgaatctc tcggtggcgg cgaccaagtc
     1441 gcggatcctg agggccaggc tggctctcag ggagaggctc tcggaagtat ttaaggagaa
     1501 agcaaatgag cgagtgtaaa aaacatatac aaagtatagc agattatatc gacggcgaac
     1561 tcgggccaga actttgcagc gagattgaag agcatcttaa aggttgtaaa aactgccgcc
     1621 tgatggtgga cacgctcagg cagacggtga ttctctgcaa agatggggag agggaggaac
     1681 ttccggagtc gatcagttca aaattgaatg aggccattaa aaggcgctgg gaaaagaagt
     1741 tcggcaaagc ctgatttata actctgacag ctaatagaaa atgcaaaatt cagttccgtc
     1801 ctgtccgtag acttggcggt ttttttgtga attatttggt tttatcgtaa cgcagggtta
     1861 caattcgcct ccggcaaacc aagactctgc aaaacgaaaa agtgctttgt aaatggtcaa
     1921 cctgagggag cgaagtgacc aaagaatctc tcatgaacag acagattttt gttcgtcaaa
     1981 ggccggttct gtaacttcgc tcaaagcgac ctggcgtata agccataata gcgcaagcaa
     2041 ttagcactaa ttcaaaaaaa tctgaatcat ttcaagtcaa tccttatcta aacggttaaa
     2101 tataatttcc aaaaaatccc aaaggagaga cattatgaag aaggcactta ctgaattttc
     2161 tattaatcat ccctggatag ttatcggcat cgtagctgtt atcactgtat tcttcgcgct
     2221 gcagctcccc aatattaaaa ttgacactga tcctgaaaat atgctcgagg aagatgagcc
     2281 tgtgagagtc ttcgaccgcc aggtcaagga agaatttggg atttcggatt ttatcgcagt
     2341 aggcgtagtc tcccaggagg gagctttcaa tgaagagatt cttaccaatg tctataatat
     2401 aattgaagag atcgaggata ttgacggcgt tattgccgac gatatcatgg ccccctcaac
     2461 tgtggatgac atcaagcaga ccccggatgg aatcctgaaa gttgaaactc tgcttgaagg
     2521 ggagcctgag tccgattcgg cggcccgcta tgttctgggg cggatcaagg gcaatccgat
     2581 tctgcgcggc aagctgggtt cggatgacgg caaagcgatc gccatgttca tcccgatcga
     2641 atccaaggat atgtcctacc gtatatccaa tgaaatcaaa gctattatag acaagcattc
     2701 taacggcgag gaatattaca tcgctggcct gcctatcgcg caggacagtt tcggggcgga
     2761 gatgttcaag cagatggcga tttctgctcc gatggccggg gcaatcattt ttctgctgat
     2821 gctgctcttt ttccggcaga taaaggttgt tattcccccc atgatcgtgg cggtagtgtc
     2881 ggtcatctgg gccatggggc tcctgattgc gacagggaac acggtgcaca ttatgagctc
     2941 gatgatcccg atattcctga taccgatcgc agtactaaac tcaatacata ttatatctga
     3001 gtttcatgac cgttatcaaa agcacaagca caaaaagacc acgatcagaa atacgatttc
     3061 cgaactgttt attcccatgc tgtttacatc gctaacgacc gtggtcggat ttctttcgct
     3121 ggcgacaact ccgataccgc cggtacgggt atttgg
//
LOCUS       DSEB01000205            3229 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003510, whole genome shotgun sequence.
ACCESSION   DSEB01000205 DSEB01000000
VERSION     DSEB01000205.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003510"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="ENO07_03755"
     CDS             complement(<1..1023)
                     /locus_tag="ENO07_03755"
                     /inference="COORDINATES: protein motif:HMM:PF10503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDS01112.1"
                     /translation="MRKGYMRKKFTLILLALVVVFASCQLSADWQSDLDRLLRTSDEN
                     EREELLVSILETDPPVDAVISFIKNLRFPERPGGQLIKNMNMCIDSVERPWVLYVPEQ
                     YDPNLPTPMIVILHGMVGRKNIIDDPVKYAEEHPILEHIAREAGWLALFPFGQEGATW
                     WDEVGMANIRDLVRTVKSEYNVDDNRVHLAGFSDGASGAYGLAMLDPTDFAAFVCLNG
                     DPGVPNEDGGLHTYASNLNNVSIYAGFTNQDQLYPTASMSPAVRLIQRAHADLKFQAF
                     EGEHDLAAYGPQDFEQMLGFMNNNKRQILSEFNWETAEPEFGEFQWFAIDEISKGAPE
                     RWHTVLN"
     gene            1163..2071
                     /locus_tag="ENO07_03760"
     CDS             1163..2071
                     /locus_tag="ENO07_03760"
                     /inference="COORDINATES: protein motif:HMM:PF00892.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01113.1"
                     /translation="MAAIGGHFLIIIAGVFYGTIPVFATLLSRYEVSTTEQVFVRLAL
                     SVSIFLAYVLLKGRHSLRLNPRDYFHFFIFGLAGIALFFTLYMTAAVMASVTVAVLLL
                     YTQPIFTLILSRILYRRQIMLSGYLAILLALIGVAAIFRVWDISWADFGLGHIFALMT
                     GFLYSVYIIFMSRKTPKYDTLTVTFWAFLFGLVWMLPLWFLTHLVFKNPVMTGLDFSL
                     PVNTWLLLIAFTLCTNIIAYLAFNHGIRTVEPHRAGVLVLAEPLVAIILGAALLGQTL
                     YLTDLIGGILILVSFLIVKRRRKSKP"
     gene            complement(2133..2747)
                     /locus_tag="ENO07_03765"
     CDS             complement(2133..2747)
                     /locus_tag="ENO07_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01114.1"
                     /translation="MKILYLFLIIFLAVALSCGGNGEQEETGTAEAVEEFGREQLEPE
                     REYAPVAELGEWRKITSSEGDFSFMMPGEPYYAVDTVNTPRGPVEYPSYTLDTPEKHL
                     MVGYSDMPEDLVAQAPARVVLEGTVRGVVEESGGTSVLDTLIQNENMTARESVVTLPD
                     GESVMRVRAYMSGNRIYIVTASAPVSDTAGLLGRFVESFELLKE"
     gene            complement(2785..>3229)
                     /locus_tag="ENO07_03770"
     CDS             complement(2785..>3229)
                     /locus_tag="ENO07_03770"
                     /inference="COORDINATES: protein motif:HMM:PF14534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDS01115.1"
                     /translation="EERIDFEAARESMINADEAFSLASVEEGMRPAFDRYMAENATIY
                     RDRMEPITGREEILSFYPPPDEQEGILSWEPFYAEIAESGELGYTLGKWIYTVTDTLG
                     MENEDYGYYVTIWKKQPDGSWKYVFDTGITGPEPDEEIPEDIPEE"
BASE COUNT          760 a          915 c          705 g          849 t
ORIGIN      
        1 attaagcaca gtatgccatc tttccggcgc acccttactg atctcatcta tcgcaaacca
       61 ctggaactca ccgaattccg gttccgcagt ctcccagttg aactcggaaa gaatctggcg
      121 tttattatta ttcataaaac ccagcatctg ttcgaaatcc tggggcccgt aagccgcaag
      181 atcatgttct ccctcgaaag cctgaaattt caaatcagca tgagccctct gaatcagccg
      241 cactgcggga ctcatcgatg ccgtggggta gagctgatct tgatttgtga agcctgcata
      301 tatggaaaca ttattcaaat tggaggcata ggtatgcaaa ccgccatcct cattcggcac
      361 gcccggatca ccattcaggc atacgaatgc tgcaaaatca gtcgggtcca gcatcgccag
      421 accgtaagcg cccgaggctc catcggaaaa acccgccagg tgaacccgat tgtcatcgac
      481 attatattca cttttaactg tccgaaccag atccctgata ttggccatgc ccacttcatc
      541 ccaccaggtg gccccctcct gtccgaaggg gaaaagcgcc agccatccag cctctctggc
      601 aatatgctcc aggatcggat gttcctctgc atatttcaca ggatcatcaa ttatattctt
      661 tctgccgacc atgccgtgaa gaatcacgat catcggggtg ggcagattcg gatcatactg
      721 ctccggaaca tacagcaccc atgggcgctc cacactatcg atacacatat tcatgttttt
      781 tataagctgt ccgccgggcc tttccggaaa tcgcaaattc ttgatgaagg agattacggc
      841 atcaacaggc ggatcggtct ccagaattga aaccaggagc tcttcccgtt cattttcatc
      901 cgaggttctc agcagccggt ccagatctga ttgccaatcg gctgataatt gacagctcgc
      961 aaaaacaact accagggcca gcaagattaa tgtgaacttt ttgcgcatat atcccttccg
     1021 cattaataaa cagctttatt agaatattga aaaaatatat cttgaatgca aaccttaata
     1081 aaaattaatt acagcgcgga aggtatttgc tcggtaaaca gataaggctt ttagaagcat
     1141 actgggagtg caaaaataag taatggctgc tattggggga cattttctta taataattgc
     1201 cggagtattc tacggcacta tccctgtttt tgcaactctc ttgagccgtt atgaggtctc
     1261 caccaccgag caggtgtttg taaggctggc attgagcgtg agcatcttcc tggcatacgt
     1321 gctcctgaaa ggaaggcaca gtctcaggct gaatccgcgg gattatttcc atttctttat
     1381 cttcggcctg gccgggatcg ccctcttctt taccctttat atgaccgcgg cggtgatggc
     1441 ttcggttacc gtggcggttc ttcttctgta cacccagccg attttcacgc tgattctctc
     1501 ccgcattcta tacaggcggc agatcatgct ctccggttat ctcgctatct tattggcgct
     1561 tatcggggta gctgcaatct tccgtgtctg ggatatttcc tgggccgact tcggactggg
     1621 gcatatcttc gctcttatga ccggctttct ttattcggtc tatatcatct tcatgagccg
     1681 caagacgccc aaatatgaca cgctgacagt caccttctgg gcatttctct tcggactggt
     1741 gtggatgctg ccgttgtggt ttttgacgca cctggtgttc aaaaatccgg tcatgaccgg
     1801 tcttgacttc agtcttccgg tcaatacctg gcttttgctt atagccttca cactctgcac
     1861 caacatcatc gcttacctgg ccttcaacca tggaattcgc acggtcgagc cgcatcgcgc
     1921 cggagtgctg gtgctcgcag agcctctggt ggcgataatc cttggcgcgg cgcttctcgg
     1981 ccagactctc tatttgacag atttaatcgg cggtattttg atactggtgt catttttgat
     2041 tgtcaagcgg aggagaaaat ccaaaccgtg aaagttgcgg cagatcctca gatctgccgc
     2101 aaattattat actctcaacc catccgaccg aactattctt tcaagagctc aaacgattcc
     2161 acaaatcgtc ccagaagacc ggcggtgtcc gataccgggg cggaagccgt cactatataa
     2221 atccggtttc ctgacatata ggcccggaca cgcatgacag actcgccgtc cggcagcgtc
     2281 acaacacttt cgcgcgcagt catattctcg ttttgaatta acgtatcgag cacactggtt
     2341 ccgcctgatt cctcaaccac cccgcgcaca gtcccctcca gcacaactct ggccggggcc
     2401 tgggccacca aatcttccgg catgtccgaa taaccgacca tgagatgctt ttccggggta
     2461 tcgagagtat aggatgggta ttcgaccgga ccacgcggag tattcacggt atcaaccgcg
     2521 taatacggtt cgccgggcat catgaagcta aaatcgccct cactcgaggt aatcttcctc
     2581 cattccccca attcagctac aggcgcgtac tccctttccg gctccagctg ctcccggcca
     2641 aactcttcga ctgcctcggc cgtcccggtc tcctcctgtt ctccgtttcc accgcaagaa
     2701 agtgcaacgg ccaaaaaaat tatcagaaat agatataaga ttttcatcct gttctccttc
     2761 ccttttagcg agctttaaaa aagcctattc ttccggaatg tcctccggaa tttcctcgtc
     2821 cggctcagga ccggttatac cggtatcgaa cacatacttc caggaaccat ccggctgttt
     2881 cttccagata gtaacataat aaccataatc ctcgttttcc atccccaaag tatccgtcac
     2941 agtgtaaatc cattttccca atgtatatcc caattcgccg gactcggcaa tctcggcata
     3001 aaacggctcc cagctcaata tgccttcctg ttcatccggc ggcggataaa aggacaaaat
     3061 ttcctcgcgc cctgtaatcg gctccatcct gtcgcggtaa attgttgcat tttccgccat
     3121 ataccggtcg aaagcgggac gcatgccctc ttcaaccgac gcgagggaga aggcttcgtc
     3181 ggcgttgatc atactctcgc gggcagcctc gaaatcaatt ctctcttcc
//
LOCUS       DSEB01000206            2524 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1006037, whole genome shotgun sequence.
ACCESSION   DSEB01000206 DSEB01000000
VERSION     DSEB01000206.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1006037"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            108..1856
                     /locus_tag="ENO07_03775"
     CDS             108..1856
                     /locus_tag="ENO07_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01116.1"
                     /translation="MRSSAIFLILLAFVVSSAAKVDAHSADLNYKKYMSDKYTFGQDK
                     SGGTELSGSNNTFRLHSQIDREHSEDASRPEGDTESHPTYTSSDMPNLQIRVHKVGNI
                     HMTVTNYGMFGDRISDSFIDPETGLPAPSCEYPAGSGNEYIFQGGLWIGAIVGDDTLV
                     TVGVDGWQNVNEMFPEGPPEGDIEKRSTIPSSPSYSPDAISEADYIAIYYDTLTDPTY
                     VISDPFDARPHLPLGLKIRQESYSWSDPEYDDFIIFRFIISNIAQNQLNEIYFGFYCD
                     ADILNPEINPGGFSDDISGSIQYTDPLTWENVLIGWSSDNDGDPDGGSWDYSSPRGAI
                     GVSLLDFPAPPYSSFNWWVPALYNPATYDWGPMLDSNYRDFGTGGIGTPSGDRNKYYI
                     MANAELDYDQMFAAIDHTGEEWLPPPVSTFALNLANGYDARFLFSFGETDLSPGNSMD
                     FAFVLTIGDEFHKNPDDYSNLFDPQNPQLFYDSLDFSNLIDNVQAARQLYRSLFPGEY
                     SCGDANNDGLVNLNDAIWIVDYVFIGGLEPYHFISGEVNCDGLVDVSDAVWIVNYLFL
                     SGNDPCDLDGDGIPDC"
     gene            complement(1936..>2524)
                     /locus_tag="ENO07_03780"
     CDS             complement(1936..>2524)
                     /locus_tag="ENO07_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HDS01117.1"
                     /translation="IKGGKNVDEDIERVMKVREKVGKSIELRFDANQGYSLAEAKRFV
                     EKTRAAKLELLEQPTPKGQPDLLGRVTSEVSIPVMADESLLTLRDAFRLARRDLADMI
                     NIKLMKVGGIFNAVQINAVARAARLESMVGCMDEAGLAIAAGLHFALARPNVVYADLD
                     GHLDLIDDPTAGAVILKNGILSPTQKAGLGFDLKD"
BASE COUNT          618 a          618 c          583 g          705 t
ORIGIN      
        1 atataatagt tgccggtaga tattatctgt ttccgtcaaa agctttgggc ggctctgatc
       61 gcactgaaaa tatttgtcgg cacagctgca gaaaatcgga ggacacaatg agaagctcag
      121 caattttcct gattctttta gcttttgtcg tctcgtccgc agcaaaggtc gatgcacaca
      181 gcgccgatct caattacaaa aagtacatgt cggacaaata tactttcggg caagacaaat
      241 caggcgggac agagttatct ggctctaata atactttcag gttacattca caaattgacc
      301 gagagcattc agaggatgct tcccgccctg aaggcgacac tgaaagtcat ccaacttata
      361 cctcatcgga tatgccgaac ttacagatta gagttcacaa agttggaaat atccatatga
      421 ctgttaccaa ttatggcatg ttcggcgacc gcatatcgga tagctttatc gatcctgaaa
      481 caggcctgcc cgcgccatca tgcgagtatc ccgcaggatc gggaaatgaa tatatcttcc
      541 agggcggatt atggatcggc gccattgtgg gtgatgacac gcttgtcaca gttggcgtcg
      601 atggctggca gaatgtgaat gagatgtttc ctgaagggcc tcccgaggga gatatagaga
      661 aacgttcaac tattccatcc agcccatcct attctccaga tgcgataagt gaagcggatt
      721 atattgctat atactacgat accttaaccg atccaactta tgtgatatca gacccttttg
      781 atgcgcgccc tcatctccca ctgggtctta aaatccgaca ggaatcatat tcatggagtg
      841 accccgaata tgatgacttc ataattttca ggttcatcat ttccaatatc gcccaaaatc
      901 agctcaatga gatttatttt ggattctatt gtgacgccga tatactaaat cctgagatca
      961 atcccggcgg attttcggat gatatctccg gttccattca gtataccgac ccgttaacct
     1021 gggaaaatgt attgattgga tggtcttcgg ataatgacgg agacccggat gggggaagct
     1081 gggattacag ctccccgcgc ggggccatcg gagtatctct tcttgatttt ccggcgcctc
     1141 cctattcgtc ttttaactgg tgggttcctg ccttatacaa tcccgccaca tatgattggg
     1201 gtcccatgct tgattcaaat tatcgcgatt tcggtacagg cgggataggc accccctcgg
     1261 gagatagaaa taaatattat ataatggcta acgccgagct tgattatgat cagatgtttg
     1321 cggccattga ccatacaggc gaagaatggc tgcctcctcc ggtcagcact ttcgccctaa
     1381 atttggctaa tgggtatgac gcgcgctttt tattttcatt cggggaaact gatttgtctc
     1441 cgggcaactc gatggatttc gcatttgtgt tgacaatcgg tgatgaattc cacaaaaatc
     1501 ctgatgatta cagtaatttg ttcgatcctc agaatccgca acttttctat gattcactgg
     1561 atttcagcaa tctgatcgat aacgtccagg ccgcaagaca attataccga tctctttttc
     1621 ccggagaata ttcctgcggc gacgccaata atgatggcct ggttaatctg aacgatgcca
     1681 tctggattgt agattacgtg tttataggcg gtttagaacc ctatcatttt atatcaggag
     1741 aggtgaattg cgatggtctt gttgacgtca gtgatgcggt ctggattgta aattatctat
     1801 ttttaagcgg gaacgatccc tgcgatcttg atggggacgg tattccggat tgctgatgta
     1861 aatttacatg cacggcctac tcctttatac ccgctcagaa atgggcgggt attttttgaa
     1921 tcaggcaagt gtttgtcaat ctttcaggtc gaagcccagt cccgcttttt gtgtggggga
     1981 aaggatcccg tttttgagga tgaccgcccc ggcagtgggg tcgtcgatca ggtccagatg
     2041 accgtccaga tctgcgtaaa caacattggg acgtgccagg gcaaaatgca gtcccgcagc
     2101 aatcgcaaga ccggcctcat ccatacatcc caccatgctc tccagtctcg ccgcccgcgc
     2161 tacggcattg atctgcacgg cattgaatat tccgcccacc ttcattaact tgatattaat
     2221 catgtcggcc agatcacgtc tggccagccg gaatgcgtcg cgcaacgtaa gcaggctctc
     2281 gtcagccatc accggaatac tgacttcgct tgttacccgc cccaaaagat cgggctgtcc
     2341 tttgggtgta ggctgttcca aaagctccag cttcgccgca cgggttttct ccacgaatcg
     2401 cttggcctct gccagggagt agccctgatt ggcgtcgaat ctgagctcta tactttttcc
     2461 aactttctcg cgaactttca ttaccctctc aatatcttca tcgacattct ttccgccctt
     2521 gatc
//
LOCUS       DSEB01000207            2708 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1005158, whole genome shotgun sequence.
ACCESSION   DSEB01000207 DSEB01000000
VERSION     DSEB01000207.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1005158"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="ENO07_03785"
     CDS             complement(<1..1067)
                     /locus_tag="ENO07_03785"
                     /inference="COORDINATES: protein motif:HMM:PF07396.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01118.1"
                     /translation="MAGNSQLRRSGAKLIAVIVLILCIIHSPASAIGPFEIADSDSSA
                     ILRFQLAGQFQTLWESRDRGDDSGKENSVYMKVRRVRPTLMVTVPDYKTSFKLHLSAA
                     PGAVELMDLYFDSELLSFFAFRIGQYKIPFTRYRIQSFQRLTLVDWAIVTKYFGAERQ
                     MGIAVHNGYEKPPRLAYVAGIFNGVNARASHTSGLASLFGEKVINRSDLSASSPRSEF
                     HPELVFHFAYNANSINVRSDSDAGGGGLRYSIASSIAWDIDPTEYHDLALRAAQEFLV
                     KYEGFSVMGAGYLGFSGIGESLRSRLAFSGLLIQSAYRTTGRVEFSLRYAHVKIEDAI
                     ADEAFNRAQSIIAETEDDAIIS"
     gene            complement(1103..2347)
                     /locus_tag="ENO07_03790"
     CDS             complement(1103..2347)
                     /locus_tag="ENO07_03790"
                     /inference="COORDINATES: protein
                     motif:HMM:PF01272.17,HMM:PF03449.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01119.1"
                     /translation="MRTVILKDAYDTLVKRIEKIKKDIRLNSKEIGRAAELGDLSENA
                     EYDAAKEKQSELFSTLNNLETYLKARLIEEKDINTEVVSFGTRVKLFDMNRHKVVSYV
                     VAGPVEFELEIYPSIVTFTSPLGQGLIGKKKGQVVDIELPNQTSRFLILNIEPVTEEG
                     PTHPDLLILGHAGYDVSDSGSSEKKNLLGGPAYYTGVGASSLSDRTAIITSIKKDHDE
                     LYKALNNLSVFVDGINLSDDEDSFSITDIPSEYHNAKYLHISEAPPDKQLQWLKDVKK
                     GGNFEGLLSIQISDSFSKEHIYILAEILQHCDFIFTSEDGFKLMEEMDDLEIEDKVIV
                     VIKSDASTELWIDGELQLDAKGFDSDSVDSTGYKGVLAGAFLAVLSMGQVEETAYDVA
                     VQLGSKSLEDDGVEHLLKVKED"
BASE COUNT          735 a          681 c          554 g          738 t
ORIGIN      
        1 gaaataattg catcatcttc ggtctccgcg ataattgact gcgccctgtt aaacgcctca
       61 tccgcaatcg catcctcgat ttttacgtgg gcatagcgca atgagaactc aacacggcct
      121 gttgttctgt aagccgactg aatcagcaga cctgaaaaag ccagacggct gcggagcgat
      181 tcaccgattc cactgaatcc aaggtaaccc gctcccatca cggaaaaccc ctcatatttg
      241 accagaaatt cctgggcggc ccggagagcg aggtcatgat attcagtcgg atcaatatcc
      301 caggcaatgc tggaggcaat gctgtaacgg aggccgccgc ccccggcatc agaatcggaa
      361 cggacattga tagaattagc attataggcg aaatgaaaaa ccagttccgg atgaaattct
      421 gacctcgggc tggatgcgga caggtccgac ctgtttatta ccttctcacc aaacagcgag
      481 gccagcccgg aagtatgaga ggcccgggca ttgacaccat taaaaatacc ggccacataa
      541 gccagcctcg gcggcttttc atatccatta tgcactgcaa tccccatctg ccgctccgct
      601 ccgaaatatt ttgtcacaat tgcccaatcc acaagtgtga gccgctggaa tgattgaatt
      661 ctgtatcttg tgaatggaat cttgtactgc ccgatcctga atgcaaaaaa agaaagaagc
      721 tcggaatcaa aatacagatc catcagttca accgccccgg gcgcagcgct cagatgtaac
      781 ttgaatgatg ttttataatc aggaacggtg accatgaggg tcggtcgtac tctgcgcacc
      841 ttcatataaa cggagttttc cttcccgcta tcatcccccc tgtcccggct ttcccagagc
      901 gtctgaaatt gaccggcgag ctgaaaccgg agaatagcgc tgctatcaga atcggcaatc
      961 tcgaacggcc cgatcgctga agcaggcgag tggataatac acaaaatcaa aacaattacg
     1021 gcaatcaatt ttgcgcctga acgccgcagc tgactgttcc cggccataag cgccctcccc
     1081 tctgaagaag tcttttccta tcttaatcct ctttgacttt caagagatgc tcgacaccat
     1141 catcctcgag agattttgag ccaagctgca cagccacatc gtaggcggtt tcttcaacct
     1201 ggcccatcga taataccgca agaaatgctc cggccagaac tcccttgtaa ccggtagaat
     1261 cgacagaatc gctatcaaaa ccttttgcat caagctgcaa ttcaccatca atccacagtt
     1321 cagtacttgc atccgatttg atgaccacta tcactttatc ctcaatttca agatcatcca
     1381 tttcttccat taacttgaac ccatcttcgc tggtgaaaat aaagtcacaa tgctgcaaaa
     1441 tttctgcgag gatatatatg tgctccttag agaaactgtc ggagatttga attgataaga
     1501 gaccttcgaa attaccgcct tttttaacat cctttaacca ttggagttgc ttatcgggcg
     1561 gggcttccga tatatgcaaa tatttggcat tgtgatattc ggaggggata tcagtaattg
     1621 aaaagctatc ctcatcatcc gataaattta taccatcgac aaaaacactc aaattattga
     1681 gcgctttgta aagctcatca tgatcttttt taatggaagt tattattgca gttctatcgg
     1741 aaagacttga ggcgccaacc ccggtatagt atgccggccc gccaagcaaa ttctttttct
     1801 cactgctgcc ggaatcgcta acatcatatc cggcatgacc caaaataagc agatccggat
     1861 gggttgggcc ctcttccgtt accggttcta tattcaggat caggaatctg gaggtctggt
     1921 ttggaagctc gatatcgact acctggcctt ttttctttcc gatcaggcct tgccccaggg
     1981 gcgaggtgaa tgtcacgatc gagggatata tttccagctc gaattcgacc ggaccggcca
     2041 caacatagga aacgacctta tgtctattca tatcgaacag ttttaccctg gtcccgaatg
     2101 aaaccacttc ggtattgata tctttctcct cgatgagcct tgctttaagg tacgtctcca
     2161 gattattcaa tgtgctgaac aattccgatt gcttctcttt agcggcgtca tattcggcat
     2221 tctccgaaag gtcgcccaat tccgcggccc ggccgatctc ctttgaattc agacgtatat
     2281 cctttttgat cttttctatc cgttttacca gcgtatcgta agcgtcttta agaataacag
     2341 ttcgcataaa gtctcttcct tatcttattc caacattggg aatctataat attcaatgtc
     2401 ttgctgacaa aattaagcta ttcaggcaac gcctttcaaa agcgcataaa tataataaaa
     2461 aatccggaaa acgcatccct gatatgaagg aaatttggag aaaaatgttc cttaattata
     2521 ttgggttttg atgattttta aaatctattt atttttcagt tctttgtacc aattgagtgt
     2581 aacttcgatt tcgttctttc gtgtcccagt ttgattgcct ggagttacga ataaaaattt
     2641 ccgggcatcg ttggacacat cataacccca cgcactcaag ggatcaagtg aattgtataa
     2701 tcgtctac
//
LOCUS       DSEB01000208            2709 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1005155, whole genome shotgun sequence.
ACCESSION   DSEB01000208 DSEB01000000
VERSION     DSEB01000208.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1005155"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(185..1003)
                     /locus_tag="ENO07_03795"
     CDS             complement(185..1003)
                     /locus_tag="ENO07_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01120.1"
                     /translation="MADSMVFDHDGLVKLADAVSKERDKITISTDLGLTETEIDLREL
                     RIDPKIILPILKKERGIFKLENGRLRHLAVFDDGYYQLVATGEAPTVEIDGIQMHRTK
                     EVKPFEDARQKAETVVRKGDVVLDSCGGLGYTAIWAVRMGAAHVDSCEPNENIRNLRK
                     ENPWSQELYSDKIRIHEMTAQEFMQNCSDDRYDCIIHDPPRFSLAGELYSFEFYSRLA
                     EIIKPKGRLFHYTGNPYSKGRPRNFVEGVIKRLAEAGFKTQRMDQVLGVRAVRR"
     gene            1137..2345
                     /locus_tag="ENO07_03800"
     CDS             1137..2345
                     /locus_tag="ENO07_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDS01121.1"
                     /translation="MGTDIISDLAKKTDTKILYLIMDGLGDIPADGHDTPLAEAHTPN
                     MDRLAREGTLGLCDPIAPGITPGSGPAHLSLFGYDPVENNVGRGLLSAFGLDFDLEPG
                     DLAMRGNFCTIDDDGNVADRRAGRISDEENKKACKLILDNIEMPDNIKLFLKTESEHR
                     VLVVLRGDGLAEEVEETDPQAAGVPPNPPRALTSEAKKSERVLTSMLDQIKKILKDNH
                     PANFVLLRGYSNPLELPTFKDRYKLDALAIAEYPMYRGLAKLVGMKTLPAYKDFEDGI
                     SQLKKFWDDYDFFFFHVKKTDSYGEDGNFENKKKMIEEVDKGIVPAVIKLNPDVLVIT
                     CDHSTPWSLKAHSWHPVPVLFKSRHDRPDECKVFTEAECLKGSMGRHPSTDIMPLALA
                     HAGKLEKFGA"
BASE COUNT          675 a          695 c          607 g          732 t
ORIGIN      
        1 ccattgcaca atcattttaa atatatcatt caatattgca gtgtttagcg gtaatcgggg
       61 tgccgccgac aaactcgttt gtcggcgatt cactcaatac aaagatccgc cacaaaactg
      121 gtttgtggcg ggcacccaac ttataatctt ttttaattac ttcaggctat ccttataccg
      181 aagcttatct cctaaccgcc ctcacaccca aaacctgatc catcctctgc gtcttaaatc
      241 cagcctcggc cagacgtttg atcacaccct ccacgaaatt tctgggacgt cccttggaat
      301 atgggtttcc ggtatagtga aaaagacggc ctttgggctt tatgatttct gctaatctgg
      361 aatagaactc gaatgaatac agctcccccg ccagcgaaaa ccgcggcgga tcatgaataa
      421 tgcagtcata ccgatcgtca gagcaattct gcataaactc ctgtgcggtc atctcatgaa
      481 ttctgatttt atcggaatac agctcctgcg accagggatt ctcctttcga agattgcgta
      541 tattctcatt cggctcgcaa gaatcgacat gtgccgcgcc cattcgaacc gcccagatcg
      601 cagtatatcc aagacctccg catgaatcca ggacaacatc ccctttcctg accactgtct
      661 cggccttctg gcgggcatcc tcgaatggct tgacctcttt tgtgcgatgc atctggattc
      721 cgtcaatctc aactgtcggc gcttctccgg ttgccacaag ctgataatag ccgtcatcga
      781 ataccgccag atgtcttagt ctgccgttct cgagcttgaa gatgcccctc tcttttttca
      841 agatgggcag gataatcttc gggtcaatcc gcaattcccg caaatcgatc tcggtctcgg
      901 ttaatccgag atctgtcgag atcgtgatct tgtcacgctc cttgctgacc gcatcagcca
      961 gcttcaccag cccatcatga tcaaatacca tactatcagc cataaattgc accgttccgt
     1021 caggtcctga tcccgattta tcgggaacat catcccgcaa aactctttta aattgacata
     1081 cagacctttg caatatagta tatcgaaaat ctcgaatatc aaaaggagtt ttatatatgg
     1141 gcacggatat tatcagcgac cttgcgaaaa aaactgacac caaaattttg tatctaatta
     1201 tggacggtct gggggatatt cctgccgacg gccatgatac gccgctggca gaagcgcaca
     1261 ctcccaatat ggatagactt gccagggagg gtacactcgg tttgtgcgac ccgatcgccc
     1321 ccggaatcac tcccggaagc ggccctgcgc atctgtcgct ttttggttat gatccggttg
     1381 agaataatgt tggtcgcggt ctgctgtcgg ctttcggtct ggattttgat ctggagccgg
     1441 gcgacctggc tatgcgcggg aatttctgta ctattgatga tgatggcaat gtcgccgacc
     1501 gccgtgccgg acgaatctcc gacgaggaaa ataaaaaggc ctgcaagctg atactcgata
     1561 atatcgagat gcccgataat ataaagctct tcctgaaaac cgaaagcgag cacagggtgc
     1621 tggtagtact ccgcggtgat ggtctggccg aggaggttga ggaaaccgat ccgcaggccg
     1681 caggtgtgcc gccgaatccg ccgcgtgcac tgacatccga ggccaagaaa agtgaacggg
     1741 tactgaccag catgctggac cagataaaaa agatactgaa ggacaatcac cccgccaatt
     1801 ttgtgcttct gcgcggctat tcaaatccgc tggagctgcc taccttcaag gatagataca
     1861 agctcgatgc gttagctatt gccgagtatc ccatgtatcg cggactggcc aaactggtgg
     1921 gcatgaaaac ccttccggcc tacaaagatt ttgaggatgg tatctctcag ctcaagaaat
     1981 tctgggatga ttacgatttc tttttcttcc atgtcaagaa gaccgactct tatggtgagg
     2041 acggcaattt tgaaaacaag aagaagatga ttgaagaggt tgacaaaggg attgtaccgg
     2101 ctgtgataaa actcaatcct gatgttctgg ttatcacctg cgatcattcc acgccctgga
     2161 gtttgaaggc gcattcatgg catccggtgc cggtgctttt caagtcacgc catgaccgtc
     2221 cggacgagtg caaggtcttc accgaggcgg aatgtttgaa aggcagcatg ggacgtcatc
     2281 cctcgaccga cattatgccc ctcgccctcg cccatgccgg caaattagag aaattcgggg
     2341 cataatttga ttggatgcat tgtttgattt tttgtaggtc aagccctgaa ggggcttgac
     2401 gccctctatt taatgtcaat ttcactccgt attatatatt tccgattcct ttacagcttt
     2461 ctgataaaag ttggcggcca tttcatagat ttcctgtggt gataatcgat ttcgatcggg
     2521 aataaaaaga ccatatttct gaactctctc aataggattc atccattccc tataatcgct
     2581 ttctccttcc gcaaacttca gcacactcat tgctcttatt gtatgagtta tgatgtactt
     2641 gatttcttca ttcgatgaca aagcttctat ctcttttttt attaaaccat ggttctgctc
     2701 agcaaactc
//
LOCUS       DSEB01000209            2319 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1007293, whole genome shotgun sequence.
ACCESSION   DSEB01000209 DSEB01000000
VERSION     DSEB01000209.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1007293"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..295)
                     /locus_tag="ENO07_03805"
     CDS             complement(<1..295)
                     /locus_tag="ENO07_03805"
                     /inference="COORDINATES: protein motif:HMM:PF00146.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HDS01122.1"
                     /translation="MLEIAIISTIKVIFVFVALLTACAYMTLIERRVVARFQTRLGPI
                     YAGPNGLLQPVADLMKLIFKEDIIPEHVDKKIYTIAPLAAFIPATLSIAVIPFG"
     gene            complement(297..>2319)
                     /locus_tag="ENO07_03810"
     CDS             complement(297..>2319)
                     /locus_tag="ENO07_03810"
                     /inference="COORDINATES: protein
                     motif:HMM:PF01568.19,HMM:PF04879.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01123.1"
                     /translation="YSGNVVEYCPVGALTNRDWRYKVRVWKTVEFPTICRYCSDGCNL
                     RVSVFRNKIYRAQSRANWSIDEGLPCDLGRYGYQFAHHDDRLTKPMIRKDDELVESSW
                     GEALTVIKNKIDETVKSRGSSGVACLVGESLSNEDYYTIGRFFRSVIGTNSIDHRIYR
                     KKKLSFGKDVVERELCWNKLDIADLENASQFFIVGSDLHSENPITALRVLKAKRYSNA
                     RIMLLNPLPTHLNRAADEEHIYNAYSEVFLLQGLARLIVESKLYDPEKANLREDEAKL
                     FLKETAEFELKTVSEKTGLAPVVLEALARSLCKTEKTVFISGENVRIHYQRDPILIAL
                     YNLAKLTGNLNGNSCGVLLLGGESNSMGCYLFGMRPDRLPYDKPFEKAEELKAVWGDI
                     ATDQPGNDTIDILQKINDEKIECAFLLGLDAVNHYPDSEFINRTLSKLDFMVVADLFL
                     TDTARLADVVLPISSHFETDGTFVNWEGVMQKYTKSIKNIGESMPLWQIFAKLSAMFD
                     ADFKFRTADDVFRQYAPMIGRDGISRMATLPEKGLRFEKKKEYRDAGLMAVSYRQPSS
                     EELPLTVLSGNGDHHFSRNFSTRCESLNNFLADPYIAINDKLAEDMTISEGDLIKLEN
                     SSGKIVAKALIWQNLPQDVVFVPDNFLEVKVNSVRSKDNDIDKVKLVKM"
BASE COUNT          582 a          569 c          539 g          629 t
ORIGIN      
        1 atccaaatgg gatgactgct atcgacagag tagctggaat gaaagcagcc aggggcgcga
       61 tggtatagat cttcttatcg acatgttcgg gaattatatc ttctttaaat atcagtttca
      121 tgaggtcagc cacaggctgg agaagaccgt tgggccccgc atagatgggc ccgagacgcg
      181 tctggaaacg cgccaccacc ctgcgttcaa tcagcgtcat gtaagcacag gcggtcagca
      241 gcgcaacaaa aacgaagata accttaattg tggatatgat cgcaatttcc agcatactac
      301 atcttcacca gttttacctt atcgatatca ttgtctttac tgcgaacgct gttgactttg
      361 acctccagga aattatccgg aacaaagact acatcctgcg ggaggttttg ccatattaaa
      421 gcttttgcca ctatttttcc agaggaattt tcaagtttta tcaggtcgcc ctcagagatt
      481 gtcatgtctt ctgcaagttt gtcgtttatt gctatatatg gatcggccag aaaattattc
      541 aggctctcgc agcgtgtgct gaaattccgg ctgaagtgat gatccccgtt tccgctgagc
      601 acagtcaggg gcagctcctc tgatgacggt tgccgatagc ttacagccat cagaccggca
      661 tcgcgatatt ccttcttttt ctcgaacctt aatccttttt ccggaagggt tgccatcctg
      721 ctgataccgt cccgtccgat cattggcgca tactggcgga agacatcatc cgcagtccga
      781 aatttgaaat cagcatcaaa catcgcggat aatttcgcaa atatctgcca gagcggcatc
      841 gattcaccga tattttttat agattttgtg tatttctgca taacgccttc ccagtttaca
      901 aaggtgccgt cggtttcgaa atgggatgaa atcgggagaa caacatcggc aagccgtgca
      961 gtgtctgtca aaaacagatc ggccacaacc atgaaatcaa gtttggaaag tgtcctgttt
     1021 ataaactcag aatcaggata atgattgacg gcatcgaggc caagcaggaa tgcgcattca
     1081 atcttttcat cattgatctt ctggagaata tcgatggtat catttccggg ctgatcggta
     1141 gctatatcgc cccagaccgc ttttaattct tcagctttct cgaatggctt atcatacggc
     1201 aggcggtccg gacgcatgcc gaacagatag cagcccatcg aatttgactc tccgcccagt
     1261 agtaaaacgc cgcaggaatt gccgttaaga ttgccggtca gtttagccag gttgtaaagc
     1321 gctattaata tcgggtcgcg ctgatagtgg attcttacat tctcgcccga aatgaagact
     1381 gtcttttcgg ttttgcacaa tgatctggcg agcgcctcca gaacgaccgg cgccaggcct
     1441 gttttttcac tgacggtttt gagctcaaat tcggcagttt ctttcaggaa taattttgcc
     1501 tcgtcttctc tgagattggc tttctctgga tcatatagtt tactctcaac aatcagcctg
     1561 gccaggccct gcaacaaaaa gacctctgaa taggcgttat atatatgctc ttcatcggcg
     1621 gcgcgattca gatgcgtcgg cagaggattg agaagcataa tacgtgcatt ggaatatctt
     1681 ttggccttga gcacgcgcag cgctgtaatc ggattctcgg aatgcagatc ggaaccgaca
     1741 ataaaaaatt gactggcatt ttcaaggtcg gcaatatcca gtttattcca gcataactcc
     1801 cgctcaacaa catccttgcc gaagctcagt ttctttttgc ggtatattct gtgatcaatg
     1861 gaatttgtgc cgatgacaga tctgaaaaat ctgccgattg tataatagtc ttcatttgac
     1921 agcgattcgc ccacgaggca ggccaccccg gatgagcccc ggcttttcac ggtttcatcg
     1981 atcttgttct ttatgacggt aagtgcttca ccccatgaag attcaaccag ctcatcatct
     2041 ttgcgaatca tgggttttgt caggcggtcg tcgtgatgtg caaactgata accgtagcgt
     2101 cccagatcgc acggtaaacc ctcatctatg ctccagttgg cacgggactg ggcgcggtaa
     2161 attttattgc ggaataccga aacccgcaga ttacatccat cagaacaata gcggcagata
     2221 gtcgggaact ccacggtctt ccagacacgg accttatagc gccagtcgcg attggtcaag
     2281 gcccccaccg ggcagtattc gaccacgttg ccggaatag
//
LOCUS       DSEB01000210            1928 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1010968, whole genome shotgun sequence.
ACCESSION   DSEB01000210 DSEB01000000
VERSION     DSEB01000210.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1010968"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..287)
                     /locus_tag="ENO07_03815"
     CDS             complement(<1..287)
                     /locus_tag="ENO07_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDS01124.1"
                     /translation="MSDHQYFEDDALGKAYDSQLMKRLLQYVKPYKKWIVLALAILII
                     STLLRLAGPVIIKLAIDEYIAVGDADGLGRLALLYAGILLVQFFATWGQIYL"
     gene            complement(284..>1928)
                     /locus_tag="ENO07_03820"
     CDS             complement(284..>1928)
                     /locus_tag="ENO07_03820"
                     /inference="COORDINATES: protein motif:HMM:PF00005.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDS01125.1"
                     /translation="PWIVKMAIDDLRQNAATYSQLRTYATLIVILAIGAGFFRFLMRR
                     TIIWGSRIFEYEIRNEFFLHLLGLPKSFYQETATGDIIARGSNDVEATRLLAGPAVMQ
                     IINSAISISVALTLMFVLSWKLTLIALTGFPLLSILTNRLGTKIHQKSYQIQQHFSTM
                     TSYVQENVSGVKVVKAYNQEEDQIKNFAGLNKTYIDLNLSLARIRGLFMPSIYGIVGI
                     IILLLLYFGGKQVINGSITLGTLVAFIFYLMSIMWPMLAVGWVVALYQRGTASLERIE
                     KIMRRKSEVADTDVDPNARPQLGEIEIRDLSFRYPNDDQEVLKDVNLRIPSGKTVAIV
                     GPTGCGKSTLVELILKAYKVPDGKILIDDIDINRIPMANLRRAVGYVPQETFLFSRTL
                     KENIAFGEEKIIEEEIEKAAGIAGIRQEISEFPRGFETRLGERGVTLSGGQKQRTALA
                     RAIIANPPILILDDAFSAVDTNTEELILRELKNVLETRTSILISHRISTVKNADMICV
                     MEEGRIEACGTHEELIEKSGLYAHIVELQSLEEELEALR"
BASE COUNT          496 a          516 c          430 g          486 t
ORIGIN      
        1 aggtagatct ggccccatgt agcgaagaac tgcaccagta gaatcccggc ataaagaagc
       61 gccagccggc caagtccatc ggcatctcca accgcaatat attcgtcgat cgcaagctta
      121 attataaccg gtccggcaag gcgcaggagg gtcgagataa tcagtatcgc cagggccagc
      181 acaatccact tcttgtaggg cttaacatac tggagaaggc gtttcatcaa ctgggaatca
      241 taggctttcc ccagggcatc atcttcaaaa tactgatgat cgctcatctc agtgcctcca
      301 gctcctcttc cagagattga agctcaacaa tgtgcgcata taatccggac ttctctatca
      361 gctcctcatg agtcccgcag gcctctatcc tgccctcctc cataacgcag atcatatccg
      421 catttttgac tgtcgatata cggtgtgaaa tcagaattga ggttctggtc tccaacacat
      481 ttttcaattc gcgtaatatt agttcttcgg tattggtatc aaccgccgaa aaagcgtcat
      541 ccagaatcag aatcggggga ttggcaatga tagcacgcgc cagggcggtc cgttgcttct
      601 gacctcccga caatgttacc ccgcgttcgc ccagccttgt ctcaaagccc ctggggaatt
      661 cgctgatttc ctgcctgatt ccggcgattc cggccgcctt ttcgatttct tcttcgatga
      721 tcttttcttc accgaaagcg atattttctt tcaaggtccg cgagaataga aatgtctcct
      781 ggggcacata gccgaccgcc ctccgcagat tcgccatagg gattctattt atatcgatat
      841 catcgatcaa tatctttcca tccgggacct tataagcctt gagaataagc tcgaccagcg
      901 ttgattttcc gcatcctgtc ggtcccacga tggcgacggt cttgccggag gggatcctga
      961 ggttcacatc cttcaaaacc tcctgatcat cattgggata tctaaaggac agatccctga
     1021 tttcaatctc gcccagttgc ggccgggcat tcggatcgac gtccgtatcg gcgacctcag
     1081 acttgcgtct catgatcttc tcgatacgtt cgagcgaggc ggttccgcgc tgatagagcg
     1141 caaccaccca tccaaccgcg agcatgggcc acataatgct catgagataa aatataaatg
     1201 ccaccaatgt ccctaatgtt atcgagccgt taatgacctg tttaccgcca aaatagagca
     1261 ggagtaaaat tattattccc acaataccat aaatcgaagg cataaatagc ccccgtattc
     1321 tggccagtga gagattcaga tcaatatagg tcttattcag gcccgcgaag tttttgatct
     1381 ggtcctcttc ctgattatag gcctttacca ccttgacccc ggagacattc tcctgcacat
     1441 acgaggtcat tgtggaaaaa tgctgctgaa tctgatatga cttctggtga atcttcgtac
     1501 ccaggcgatt ggtcagtatg gacaaaagcg gaaaaccggt caatgctatc agcgtcagtt
     1561 tccagctcag cacgaacatc aatgtcaacg ccacagaaat ggatatggct gaatttataa
     1621 tctgcataac agccggcccg gctaaaagac gtgtggcttc gacgtcattc gaaccgcggg
     1681 cgataatatc cccggtcgca gtttcctggt aaaacgactt tggcaggccc agaagatgca
     1741 ggaagaactc attacggatt tcatattcaa atatccggga accccagata atagtacgcc
     1801 gcatcagaaa acggaaaaat ccagcgccga ttgccagaat cactatcaat gtagcatatg
     1861 ttctaagttg cgaataggtt gcggcatttt gcctgaggtc gtcaatggcc atctttacaa
     1921 tccagggc
//
LOCUS       DSEB01000211            2050 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1009552, whole genome shotgun sequence.
ACCESSION   DSEB01000211 DSEB01000000
VERSION     DSEB01000211.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1009552"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..268
                     /locus_tag="ENO07_03825"
     CDS             <1..268
                     /locus_tag="ENO07_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01126.1"
                     /translation="SKGLIRLITKYDKRQDKCIISVEDNGPGIPENLIDKIFEPFFST
                     KGTNGLGLAVSWGIVERHRGTIEVDMADEGGAIFNIVLPAYHGT"
     gene            308..1102
                     /locus_tag="ENO07_03830"
     CDS             308..1102
                     /locus_tag="ENO07_03830"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00072.22,HMM:PF01597.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDS01127.1"
                     /translation="MEDKLNVLVVDDEQIVLDSVRKHLKKEDKYEVFSALSVRQASEI
                     MLDNEMHIILTDLMMPDIDGLEFMSMVKRDNPHIPVIVITGYATINTALQSTQLGAFD
                     YIAKPFTKKELLGVLTRAADLVRAAEKAQVNSESPGGEVTEVDIRESKMFKNIDDHSW
                     LMMRQDETVLLGVKRTFLHTVGKIESIHLPDEGDELRQGGVFLQIISADLQSHSLVAP
                     LSGTVVEVNEKVLKNPELSLQDPYGEGWLIRLKPSKFEYEIKMLGL"
     gene            complement(1111..1761)
                     /locus_tag="ENO07_03835"
     CDS             complement(1111..1761)
                     /locus_tag="ENO07_03835"
                     /inference="COORDINATES: protein motif:HMM:PF02080.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDS01128.1"
                     /translation="MKNFIVIGLGNFGSTVVRELSKLKRKVAAIDIDKAKVQAVRELA
                     HLAIIADATHKEFTENLNVDNYDCAIISTGKDSLAAILITLHLKELGVKKIIAKANSA
                     DHAKVLTRVGADEAMIPEENMAIRIAHSLARSNLVDFLPLSAGHYVAEIAAPEKFVGK
                     TLAELDLSRKYQVQVIASKGAKSGEFQFAPGGGYRVKEKDLLVVLGKEENIEKIRE"
     gene            complement(1769..>2050)
                     /locus_tag="ENO07_03840"
     CDS             complement(1769..>2050)
                     /locus_tag="ENO07_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter"
                     /protein_id="HDS01129.1"
                     /translation="VIMLVALMTLQEEPDMHGLAHGWFISNAFEVISAFGTVGLSLGT
                     TNQLETGGKIIITILMFLGRVGLLTLVFSLAGPSRHREAVYLEEQIMIG"
BASE COUNT          525 a          494 c          515 g          516 t
ORIGIN      
        1 cagcaaggga cttattagac ttattacaaa atatgataag cgtcaggaca agtgtattat
       61 ttcggtagaa gataacgggc cgggaattcc tgaaaacctg atcgataaaa ttttcgaacc
      121 attcttttcg actaagggta ccaatggcct cggacttgcg gtaagctggg gcatcgtcga
      181 gcgtcatcgc gggaccatcg aagtcgacat ggccgatgag ggcggagcga tattcaatat
      241 tgttctcccg gcttatcatg gaacctgata gaatttctga ttagtaatca atcagggagt
      301 ttttgatatg gaagacaaat tgaatgttct ggttgtagat gatgaacaga ttgttttgga
      361 cagtgtccgc aaacatctaa aaaaagagga taaatacgaa gtctttagcg cgctttcggt
      421 tcggcaggca agcgaaataa tgctggataa cgaaatgcat attattctga ccgatttgat
      481 gatgcccgat atcgatgggt tggaattcat gagcatggtt aaacgcgaca atccccatat
      541 tcctgtgata gtcataaccg gctatgccac catcaacacc gccctgcaat cgacccagct
      601 gggagccttt gactacattg ccaagccctt caccaaaaag gagcttctgg gggttctcac
      661 gcgcgccgcc gatcttgtcc gggcggcgga aaaggcgcaa gttaactccg aaagtcccgg
      721 gggggaagta acggaagtcg acataagaga aagcaagatg tttaagaata ttgacgatca
      781 ctcctggcta atgatgcggc aggatgagac agtgcttctg ggggtaaaac ggacctttct
      841 gcacacagtc ggtaaaattg aatcgatcca tttgcctgac gagggggatg agcttcgtca
      901 gggaggggtt ttcctgcaaa tcatctctgc tgacctgcaa tcacattcac tggtggctcc
      961 cttatccggc acagtagtgg aggtgaatga aaaagtgctg aaaaatcccg agctttcgct
     1021 ccaggatccc tatggcgagg gctggctgat tcggctcaaa ccgtcaaaat ttgagtacga
     1081 gataaaaatg ctgggattgt agatttgatt ctattccctg atcttttcga tattttcctc
     1141 tttaccgagc accaccaaca gatccttttc cttgacccga tatccgccgc cgggagcaaa
     1201 ttggaactcc ccggatttgg cgcccttgct ggcgatcacc tgcacctggt atttcctgct
     1261 gaggtccagt tcggccagcg ttttgccaac gaatttctcg ggtgccgcaa tctcggccac
     1321 gtaatggccc gcggaaagag gaagaaaatc gaccagattc gatctggcca gtgaatgggc
     1381 aatccggatg gccatattct cctcgggaat catggcttcg tcggcgccga cccgggttaa
     1441 cactttggca tgatctgctg aattagcctt ggcaattatc tttttgaccc ccagctcctt
     1501 caggtgcaaa gtgatcagaa tagccgcgag cgagtccttt ccggtggaga tgattgcaca
     1561 gtcataatta tcgacattca ggttttcagt gaattcctta tgggtggcat ccgcaattat
     1621 ggccagatgg gccagctcgc ggaccgcctg aactttggcc ttatcgatgt cgatcgcggc
     1681 cactttgcgt ttgagcttgg acagctcgcg cacaactgta gagccgaagt tccccagacc
     1741 gattacgata aaatttttca taattacctc atcctatcat tatctgttcc tcaagataaa
     1801 cagcctctct atgcctggaa ggtccggcca gcgaaaatac cagtgtcaaa agtccaaccc
     1861 tgcccaggaa catcagtata gttatgatga tcttcccgcc ggtttcgagt tgattggttg
     1921 taccgagcga gagacccaca gtaccgaagg ccgatattac ctcgaaagcg ttgcttatga
     1981 accagccgtg agccagaccg tgcatatcgg gttcttcctg cagggtcatc agcgcaacca
     2041 gcatgattac
//
LOCUS       DSEB01000212            1933 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1010888, whole genome shotgun sequence.
ACCESSION   DSEB01000212 DSEB01000000
VERSION     DSEB01000212.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1010888"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(82..933)
                     /gene="htpX"
                     /locus_tag="ENO07_03845"
     CDS             complement(82..933)
                     /gene="htpX"
                     /locus_tag="ENO07_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HDS01130.1"
                     /translation="MNRVKTVLFLTALTILFIVIGGALGGRTGMYIALVFAGIMNFAS
                     YWFSDKIVLKMYRAQPLDPNSNHRLVRVVRNLVGRAGLPMPKVYIIPKNSPNAFATGR
                     NPKHAAVAATQGILQILDDDELEGVMAHELSHVKHYDILIGSIAATIAGAISILATMA
                     RWAAIFGGYGGGDNRNSGGIFGLLAMTIIAPIAALLIQTAISRSREFLADKGGAEISR
                     KPLALASALEKLHNASRRVPMQANPATAHMFIVNPLRGQGLMSLFSTHPPVEKRIAKL
                     RDMSYGH"
     gene            1273..1800
                     /locus_tag="ENO07_03850"
     CDS             1273..1800
                     /locus_tag="ENO07_03850"
                     /inference="COORDINATES: protein motif:HMM:PF02620.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDS01131.1"
                     /translation="MAFILKIKDLPEDVSRQEFVSSPGELSLDYEGVEVIGDVRAYGN
                     VTKSNDQVIFRGEFVAPVRMTCSRCAEEFERNLRSELVFVLTLVPEKDYQELEEEASE
                     DFYFIPEGTQEFDFSGHIREQLIVALEMKPLCREECRGICPDCGKNLNNEECICSEKK
                     IDERWLPLRDLLDRS"
     gene            1804..>1933
                     /gene="rpmF"
                     /locus_tag="ENO07_03855"
     CDS             1804..>1933
                     /gene="rpmF"
                     /locus_tag="ENO07_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDS01132.1"
                     /translation="MPLPKRRHSKTRGRKRRTHWKLSAPNLGECDNCGELKLPHHVC"
BASE COUNT          508 a          461 c          481 g          483 t
ORIGIN      
        1 atattacagc cagttcaatc ggccagttag ccacgggcca gcaccagcct ttccgatgcc
       61 gacaatcgag gttggtccga atcagtgtcc ataagacata tctctcagtt tggcaatccg
      121 cttttcgacc ggcggatggg tagagaagag tgacatgaga ccttgacccc gcagcggatt
      181 gacgataaac atatgggccg tggccggatt agcctgcatg ggaacccgcc tggaggcatt
      241 atgcagcttt tcaagcgccg aggccagagc caggggcttc ctgctgattt cggccccgcc
      301 cttgtcggcc agaaattcgc gtgatcttga aattgccgtc tggatcagca gcgctgctat
      361 gggcgctatg attgtcatcg caagcaggcc gaagatgcct ccactgttac ggttgtcgcc
      421 tccgccatag cctccgaata tcgccgccca gcgcgccatt gtggccagaa tagatatggc
      481 cccggcgatc gtggcggcaa tcgagccgat caatatatca taatgtttaa catgggaaag
      541 ctcatgagcc atgacgccct cgagctcatc atcgtcaagt atctgcaaaa tcccctgcgt
      601 cgctgcaact gcggcatgtt tcgggtttcg gccggttgcg aaagcattgg gggaattctt
      661 cggtataata tagacctttg gcatgggcaa tccggcccgt ccgaccagat tacggactac
      721 ccgcaccagc ctgtgattgg aattcggatc cagaggttga gcccgataca tcttcaagac
      781 tattttatcg gaaaaccaat aggaggcaaa attcattata cccgcaaata ccagcgctat
      841 atacatgccg gtacgccctc ccagagctcc cccgatcact ataaacagga tcgtcagagc
      901 tgtcaggaag agaacggttt ttacgcgatt cattttatta cctccataaa tatgatagaa
      961 atctgcactt ttatgtcaat taaaattccg tcaaattata tacaacatgt gcccgagata
     1021 tgttcctgaa atcgtatcgc agaatcgctt ccattcaaat agctgacatt tattttcgta
     1081 tgattgaaga cagtttggaa ccttttgtgg cttgataggt taatgctggt aaataatgct
     1141 caaaaatttt taatacacct ggtgtttaac ttcaaaagat tcgagccgat atagataagc
     1201 gggtttttta tggcagcccc ggttgcacaa atatcttgac taataccgca aaaattgtaa
     1261 aatagtaagt ttatggcgtt catcctgaaa ataaaggatt tacctgagga tgtctcccgg
     1321 caggaatttg taagcagtcc cggggaactc tctctcgatt acgaaggtgt tgaggtcatc
     1381 ggcgatgtcc gggcctatgg taatgttacg aaatccaacg accaggttat cttcaggggt
     1441 gaatttgtcg ctccggtcag gatgacctgt tcccggtgtg ctgaagaatt cgagagaaat
     1501 ctcaggtcag aactggtctt tgtcctgacc ctggtgccgg aaaaggacta ccaggagctg
     1561 gaagaggagg cctcggagga tttctatttt attcccgagg gtacacagga atttgatttc
     1621 tccgggcata ttcgggaaca gctgatagtt gctcttgaaa tgaagccctt gtgccgggaa
     1681 gaatgccgtg gaatatgccc cgattgcggc aaaaatttaa ataatgaaga atgtatctgt
     1741 tcagaaaaga aaatcgatga gcgctggctg ccgctcagag atctgttaga caggagttaa
     1801 agtatgccac ttccgaaacg acgccattcc aaaaccagag ggcggaaaag acgcactcac
     1861 tggaagcttt cagcaccgaa cctgggtgaa tgtgacaact gcggcgagct taagctgccc
     1921 catcatgtat gtc
//
LOCUS       DSEB01000213            2239 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1007883, whole genome shotgun sequence.
ACCESSION   DSEB01000213 DSEB01000000
VERSION     DSEB01000213.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1007883"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            125..1396
                     /locus_tag="ENO07_03860"
     CDS             125..1396
                     /locus_tag="ENO07_03860"
                     /inference="COORDINATES: protein motif:HMM:PF07992.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDS01133.1"
                     /translation="MEKFPKVIIIGAGFGGLFAAKSLADKPVDVLLIDKNNYHTFTPL
                     LYQVATCALDPSEIAHPIRTIFRKNRNVSFLLGEVAAIDPGNKQITVQTSKTAEQIDY
                     DYLIVAAGSQSNYFGSSDLETFSYDLKTLPDAVDLRNHALRLFEKAVWTDNPEEVEAL
                     LTFVVVGAGPTGIETAGALYELYNHVLDKEFSSRNLQARVVLVEMQETVLNAYPQDLR
                     QRAEKQLKSLGVELKLGTPVKRVQQNQVELGDGTIIPTHTLVWSAGIQGSPVANMLGI
                     ELVRGNRVEVNQQMQVIGHDQIYCIGDMSYLLDNDGAPYPQVSPVAQQQARVAAHNIL
                     AEIEGKRLQAFSYQDLGSMATIGRSRAVAWLFNRIKISGGMAWLAWLVFHLITLVGFR
                     NRLNVFVNWVWNYITYDRSVRIILDRNLQKE"
     gene            complement(1511..1990)
                     /locus_tag="ENO07_03865"
     CDS             complement(1511..1990)
                     /locus_tag="ENO07_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDS01134.1"
                     /translation="MGVSGCGKTSVGLGLSEVLGWPFYDGDDFHPQANIDKMARGIAL
                     DDNDRLPWLERLHALIREHLEGDRSLLVACSALKANYRDVLKGGREEILFVHLEGSFE
                     LVYSRMENRSGHYMKADMLQSQYRDLEPPADALTTQIDQPVDAIITEIIGKLESGSA"
BASE COUNT          582 a          591 c          518 g          548 t
ORIGIN      
        1 attttatacc aaagtaaaaa tgaatttccg attcatacta cttacaatga aatgaaccag
       61 cgaaattatc cacggcggct ggtagaatat gtccatccac aaattttgat aatcaggagg
      121 atatatggag aaatttccca aagtgatcat catcggtgcg gggtttggcg gattatttgc
      181 agcgaagtca ctggcggata agcctgtcga tgtcttgctg atcgacaaga ataactacca
      241 caccttcacg ccattacttt accaggtagc aacctgcgcc ttagatcctt cagaaatcgc
      301 tcacccaatc cgaaccatct tccgaaaaaa cagaaatgtg agcttccttt tgggcgaggt
      361 tgccgcgatc gatcctggga ataaacagat cacggttcag accagcaaaa ctgctgagca
      421 gattgattat gattatctga tcgtagccgc aggcagccag agcaattatt ttgggagttc
      481 cgacctcgaa acgttctcct atgacctgaa aacgctgcca gatgctgtgg atttacggaa
      541 ccacgccctg cgcctatttg aaaaagccgt ttggactgat aatcccgagg aggttgaagc
      601 gctgctcaca tttgtggttg ttggggcggg accgactggc attgaaactg ccggagccct
      661 ctacgagctg tataaccacg ttctggataa agagttttcg tcccggaacc tccaggcccg
      721 ggttgtattg gtagaaatgc aggaaactgt tttgaacgct tatcctcaag atttacgcca
      781 gcgcgcggaa aaacagctta aatctcttgg ggttgaacta aaactgggca ctccagtgaa
      841 acgtgtacaa caaaatcagg ttgaattggg agacggcacc atcatcccta cccatacact
      901 tgtctggtca gcaggaattc agggctcccc ggtggccaac atgctgggga tcgaattagt
      961 tcgaggaaac cgggttgaag tcaatcaaca gatgcaggtg atcggacacg atcagatcta
     1021 ttgcattggg gatatgagct atctgctgga caacgacggg gccccctacc cgcaagtcag
     1081 tccagttgcc cagcagcaag cccgagttgc agcgcacaac atcctggcgg aaattgaagg
     1141 aaaacgactg caggccttct cctaccagga tttagggagc atggccacca tcggacgcag
     1201 tcgagcagtc gcctggctgt tcaaccgcat caagatctca ggggggatgg cttggcttgc
     1261 ctggttggtt tttcacctga tcacgttggt cggtttccgc aaccgtttaa atgtttttgt
     1321 aaactgggtc tggaattaca tcacatacga ccgctccgtc aggatcatct tggatcgcaa
     1381 cctgcaaaaa gaataactct ggtttccctc ttggtatcca ataaatttca aaaaatcgca
     1441 gactgtaaat ctgcgatttt tgattctcat acagctggta attaaattaa atcatctttc
     1501 tcacttctga tcaagcactc cctgactcca gcttgccgat gatctcggtg atgattgcat
     1561 ctaccggctg atcgatctgg gttgtcaaag catcagccgg aggttcgaga tcacggtact
     1621 gactttgcag catatcggct ttcatataat gcccgctgcg gttttccatt cgggagtaga
     1681 ccagttcaaa acttccctcc agatgaacaa acaagatctc ctcccgtccc cctttcagaa
     1741 cgtcgcggta attggcctta agcgccgagc aagccacaag caatgatcgg tctccttcaa
     1801 gatgctcacg aatcaaggcg tgcagacgct ccagccaagg caggcggtca ttatcatcca
     1861 gagctatccc gcgtgccatt ttatcgatat tggcctgcgg atggaaatca tccccgtcat
     1921 agaacggcca gcccaacacc tcagaaagcc ccagtcccac actggtcttc ccgcagccgg
     1981 aaacgcccat gataatgatc gctaatgcca tcagtctggc tgccttttca gcctgtacag
     2041 catttgccgg gctcagccac gtagagaatc cctcatcacc ccctctccaa cattctactg
     2101 cgaacttgct cgtcgccgcc cagatcgctg cctgcccctt ttaggttgtt gctacgagcc
     2161 agcacaccca gaagaaaact ctgttcctgg aatattactt gaaattcata acagtttttt
     2221 caatgccgac agcagcagc
//
LOCUS       DSEB01000214            1934 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1010880, whole genome shotgun sequence.
ACCESSION   DSEB01000214 DSEB01000000
VERSION     DSEB01000214.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1010880"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(104..496)
                     /locus_tag="ENO07_03870"
     CDS             complement(104..496)
                     /locus_tag="ENO07_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDS01135.1"
                     /translation="MEEQIIATGRRKEAVATVRLKPGSGKWNVNGRELRDYIRNRQML
                     IDHIYQAYKVTETEGKFDVFCRTKGGGISGQAGAMRLALARALSKYDSTLRQPLKDAG
                     LLTRDPREVERKKYGMVKARKRFQYSKR"
     gene            complement(521..949)
                     /locus_tag="ENO07_03875"
     CDS             complement(521..949)
                     /locus_tag="ENO07_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDS01136.1"
                     /translation="MKTYIPKLSDLNKKWYVVDLDGKVLGRQAGQIARILMGKNKPQF
                     TPHLDAGDYVIVVNAQKVVITGKKMFQKRYFRYTGYPGGLRSKTFEQMISSKPEDIIR
                     SAVKGMLPKNRLGRKMMKKLFVYRGPEHPHQAQKPEPLEL"
     gene            1412..>1934
                     /locus_tag="ENO07_03880"
     CDS             1412..>1934
                     /locus_tag="ENO07_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01137.1"
                     /translation="MTKVRGIITGTILAGLILALMIIMGCSDLPTVPEQDGIFTLADF
                     ESIAARQAEFSEPEDGGGSVLIPADEGGVIPIVFSNGSEGSLEVPPNAIRSDTEISVE
                     VTEVILRDRAILQFDFQPDGLRFSTEATLTIDASAFRDADMEYVDWYYYNPDSGKWEL
                     VDRYPLSGDKVYIK"
BASE COUNT          483 a          499 c          442 g          510 t
ORIGIN      
        1 cctttggtta attcctccaa ccgcatcatc ccccggggga catcctgttt cggacgaccg
       61 cccccgaagt cagcgattgt gcgtactggt aaaaattata cttctatctc ttggaatact
      121 ggaagcgctt gcgtgctttc accattccat acttttttct ttcaacttca cgaggatcgc
      181 gtgtcaaaag tccggcatcc ttcagcggct ggcgaagtgt gctgtcgtat ttagataggg
      241 ccctcgccag agccaggcgc atggcgcctg cctgaccgct gattccaccg cccttggtgc
      301 ggcagaacac atcgaacttg ccctcggtct cggtgacctt gtaagcctgg tagatgtgat
      361 cgatcaacat ctggcggttg cgtatataat cgcgcagctc gcggccgttg acattccact
      421 ttccgctgcc cggtttcagg cggacggtag ctactgcctc tttacggcga cccgtagcta
      481 tgatctgctc ttccatatta tcctttcaaa tccagattga ttacaattcc aggggctccg
      541 gtttctgagc ctggtgcgga tgctcgggac cgcggtaaac aaaaagcttc ttcatcatct
      601 tgcgtcccag gcggtttttc gggagcatac ccttcaccgc tgaacggatg atatcctccg
      661 gtttggatga aatcatctgc tcaaaggtct ttgatctcaa tcctccggga taaccggtat
      721 aacgaaagta tcgtttctgg aacatctttt ttccggttat tacaactttc tgtgcattta
      781 ccacgataac ataatctccg gcatccagat gaggggtaaa ttgcggctta ttcttgccca
      841 tcaaaatacg ggcaatctgt ccggcctggc gtccaagcac cttgccgtcc aaatccacaa
      901 cataccattt cttgttcaga tctgaaagct ttggtatata cgttttcata aaactcctct
      961 aatcccggaa ataattaaac gcaaaattta cattatattc gcaaaatgtc aagcattaat
     1021 aaagccatat tttggttaat ttcaagcatt gtcttaatct ttccatcaaa tacccggcaa
     1081 caacttaacc tctgccgagg ttcccctctc cgactaccgc aggcagactt tccgtaaaat
     1141 caggcaaacc accggggatt tagcgtgccc cgaatttgcc cagagcttga acagctcatg
     1201 gccagatacg acatctaccg actaattatg ggatgcttcc cacaaaaaac ctcaattttt
     1261 gaactgttca cgctccggac agctctcgga aaaagcggta ttcttatgtt taacaacgga
     1321 ttgcaaattt gtgaacaaaa tgttttagaa cggaacaggg attgcacaaa taaacaccag
     1381 aaggaggaat agaatgcaag aggtgtttat tatgacaaaa gtaagaggta tcataacagg
     1441 aacgattctg gcgggcttga ttctggccct gatgattatc atgggatgtt cagatctccc
     1501 caccgtgccg gagcaagacg ggatatttac gctggcggac tttgaaagca tcgctgcgcg
     1561 tcaggcggaa ttcagcgagc ctgaggatgg cggcggctct gttctgattc cggccgacga
     1621 aggcggcgta attcccatag ttttttccaa cggcagcgaa ggttccctag aggtcccgcc
     1681 caacgccatc agatcggata cagagatatc ggttgaagta accgaagtca tcctgcgtga
     1741 ccgggcaata ctccagttcg atttccagcc cgacggactg cgcttttcaa ccgaggccac
     1801 ccttacgatc gacgcttccg catttcggga tgcggacatg gaatatgtgg actggtatta
     1861 ttataatccc gattcaggaa aatgggaatt ggttgacagg tatccactct caggagacaa
     1921 agtctatatt aaga
//
LOCUS       DSEB01000215            2173 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1008442, whole genome shotgun sequence.
ACCESSION   DSEB01000215 DSEB01000000
VERSION     DSEB01000215.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1008442"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..345
                     /locus_tag="ENO07_03885"
     CDS             <1..345
                     /locus_tag="ENO07_03885"
                     /inference="COORDINATES: protein motif:HMM:PF13307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01138.1"
                     /translation="EGIDLPGTQLEVLLITRLPFATPTDPIEAARMEQIQKAGKDSFV
                     TYSIPNAVLKFKQGFGRLIRQKTDTGIIIVTDNRLIKSNYGQIFLNSLPTELNVIYSQ
                     DEFLDRLRSIQA"
     gene            604..858
                     /locus_tag="ENO07_03890"
     CDS             604..858
                     /locus_tag="ENO07_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01139.1"
                     /translation="MVDINKWLGYFSISIVMIVGILIVTGMVGDFDWNIRLPVGILVF
                     TYVILRILFMRRSHRKPKTFRGLGVRRDYEKLDESKPNNT"
     gene            897..>2173
                     /locus_tag="ENO07_03895"
     CDS             897..>2173
                     /locus_tag="ENO07_03895"
                     /inference="COORDINATES: protein
                     motif:HMM:PF12895.5,HMM:PF13432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDS01140.1"
                     /translation="MNGKRLKYKGFRMQKLAILVIPLLLFFGVEMVVAEVPSDIQQAY
                     DSKNYDDAIQKIEQALKRDKRNEQLYYILGLSYLGKHDTTSAMEAIDNAVRFDSKFHN
                     ARYLLGKLYLETGKLDKARNAFEDGLDKAKDKKDKALFEDGLGLYYLQSGDTTRADIE
                     VRKAQIDDPDNMTYIMHQGDIAFAQGIYSVALNAYKKALASDSLNPEVHFRIAKSYLN
                     QRQFREALSSIERALELDSSYLEGYIIGGDIYTLYGVQQADNPEAQQSLMSNAIFYYT
                     KYLEMSGDSGRANYYRGKAYFTFNDFEKALADFETALEQGYEKEDIFSLIGRSYSGLK
                     QYNKAIEFLDQYEKSILQADPNYEWTASDVSLFLERAKAKAGIGDSASRLEARQDFEK
                     AIELDSTDAMSYYWAGLNEYYLKAYERAVDYLK"
BASE COUNT          612 a          467 c          529 g          565 t
ORIGIN      
        1 gaggggatcg atctgcccgg cacacaactg gaggtgttgc tgatcacccg gttgccgttt
       61 gccactccca ccgatccgat cgaggcggcc aggatggagc agattcagaa ggccggcaaa
      121 gattcatttg taacttattc cattcccaat gctgtcctca aatttaagca gggttttggt
      181 cggctgatcc ggcagaaaac ggataccggt ataatcatag tcaccgacaa caggcttatc
      241 aaaagcaatt atggccagat ctttctaaat tccctcccga ccgaactcaa tgtcatctac
      301 agccaggatg aattcctgga ccgcctgcga tcaattcagg cctgaggcaa acgcaatttc
      361 tgcgagaatt ttataagggt aaaaagccaa taattttaac ttgcattatc ttaagcatct
      421 aataatttag gtttttttcg gaaacaaact ctcctggagt gtattatcta taatggctgg
      481 cggcatttca tgagctccgg gggcgcttca gatttgcttg agatttaaac gattaacgtt
      541 ttcaggcgtc aattaatggg aatattagcc attcctgttt gtataatgtc tggaggaaat
      601 agaatggttg atataaacaa atggctggga tacttttcta tctccatagt gatgatagtg
      661 ggtatcctga tcgtaacagg aatggttgga gacttcgatt ggaatatacg tctgccggtc
      721 gggattctgg tcttcacgta tgttattttg cggatactgt ttatgcgcag gagccacaga
      781 aaacctaaga cttttagggg tttaggagta cgaagagatt atgaaaagct ggacgaaagc
      841 aaaccaaata atacttgaca ttcagcatgt aaaacatatt ttaaaggatt atttttatga
      901 atggtaagcg attaaaatac aaaggattca ggatgcaaaa gctggcgatt cttgtcatcc
      961 cgctcttatt atttttcggt gtggaaatgg ttgttgcgga ggttccctcc gacatacagc
     1021 aagcctacga tagcaaaaat tacgatgacg ctattcagaa aatcgagcag gccttgaagc
     1081 gtgataaacg caatgaacaa ctttattata tactgggcct gagttatctt ggaaagcacg
     1141 atactacaag cgccatggaa gcgatcgata atgcggtgcg gttcgacagc aagtttcata
     1201 atgcccgcta ccttctgggt aagctctatc tcgaaaccgg aaagctggat aaagccagga
     1261 atgcattcga ggatggcctg gacaaggcca aggataagaa agataaggcc ctgtttgaag
     1321 atgggcttgg tttatattat ctgcaatcag gtgatactac cagggcggat atagaagtta
     1381 gaaaggctca aatagacgat cccgataaca tgacatatat catgcatcag ggcgatatag
     1441 cctttgcaca gggaatttat tcagtggcct tgaatgccta caaaaaggcc ctggcctccg
     1501 actcgctcaa tccggaagtg cactttcgaa ttgccaaatc ttatctgaat cagaggcagt
     1561 tccgcgaggc cctgagctcc attgaaagag cgcttgagct ggattcatcc tacctcgagg
     1621 gctacatcat cggcggcgac atctatacat tatatggcgt acagcaggcc gataatcccg
     1681 aggcccagca gtcgctgatg tcgaatgcga tcttttatta caccaagtat ctggagatga
     1741 gcggcgattc cggacgcgcc aactactatc gcggcaaggc ctacttcacc ttcaatgatt
     1801 tcgaaaaagc gcttgctgat ttcgaaaccg ctctggagca gggctacgaa aaggaagata
     1861 ttttttcctt gatcgggcgt tcatattccg gtctgaagca gtataataag gcgattgaat
     1921 tcctggatca atacgagaaa tcgatcctgc aggccgatcc gaattatgaa tggaccgcca
     1981 gcgatgtttc tctctttctc gagagagcca aggccaaggc cggaatcggc gacagcgctt
     2041 cgcgcctgga ggcccgtcag gatttcgaaa aagcgatcga gctggattcg accgatgcca
     2101 tgtcctatta ctgggccggt ttgaacgaat attatcttaa ggcttacgag agagccgtgg
     2161 attatctcaa gaa
//
LOCUS       DSEB01000216            3009 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004096, whole genome shotgun sequence.
ACCESSION   DSEB01000216 DSEB01000000
VERSION     DSEB01000216.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004096"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..394)
                     /locus_tag="ENO07_03900"
     CDS             complement(<1..394)
                     /locus_tag="ENO07_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01141.1"
                     /translation="MKHLQFILTLGVLLLAGSFLAQSNAFAASCCDTPGDANNDGNAN
                     VSDGVYIINYVFVGGDIPSCKEKGDANCDATINVSDAVWIINYVFIGGPPPCCMDVVF
                     NDEYGARVTYEAFAGSFLEAVQVTTDPVY"
     gene            complement(565..2958)
                     /locus_tag="ENO07_03905"
     CDS             complement(565..2958)
                     /locus_tag="ENO07_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01142.1"
                     /translation="MVIVGLVGCNDDRVLEPVQFPTDAVVFRDEFGSSVTFQAFAGSK
                     LDAVDLDQVNTYTGSQALQVTVPNVGDPSGSYAGGAFTAGIARDLTQYNALTFYAKAS
                     MNATLNVAGIGNDNTGTSKFTAEVNNLPLTTEWQKYIIPIPLSDKLTTEKGLFYFAEG
                     PENGSGYQLWFDEIIFETLGTISNPQPAIPTRTIEAEVSDTITITEATVTFDVGGSSQ
                     TVSAMQGYFTFVSSNETVVNVADNGTITAAGVGTSELTATLGTVQASGTVTVEVAGQA
                     ATPTTPAPTPTVPEADAISLFSNAYTDVTVDTWSADWDMADVSDETIGSDDVKKYENM
                     QYAGIEFANPTIDVSGMTRFHMDIWTPNSTASPAKFKIKLVDFGADGVFGGGNDFEHE
                     LVFDENTSPALATGAWVSIDLPLAAFSGLITRGHLGQLIISGDLSTVYIDNIYFYDAG
                     QQTAPNIPAPAPDEDPGLVISLFSDVYSNVAVDTWSAVWDVANVEDFMIGSDNMKKYT
                     DLLFAGIEFRTSTVDASAMTHFHMNVWTPDATSSPSVFKIKLVDFGANGVYEGGSGDD
                     VEDEITLGESIMNTGIWVTIDFSLSEFSDLTTRGHLGQLIISGDPNTVFVDNIYFYDS
                     GIPEAPPVAAPTPATDAGDVISLFSDAYTDVPVDTWSAVWDTADVQNYMIGSDTVKKY
                     TNLLFAGIEFTTNTIDASAMTHFHMDLWTPDPTDAPAVFRIKLVDFGANGVWDDGGDD
                     VEHEITLDQNTMNTGSWVSIEIPFTDFVNLTTRAHLAQLIISGDPNTVYVDNIYFHR"
BASE COUNT          786 a          756 c          726 g          741 t
ORIGIN      
        1 catatacggg atccgttgtt acctgaacag cttcaagaaa agatcctgca aaggcctcgt
       61 acgttaccct tgcgccgtat tcatcattga ataccacgtc catacaacag ggcggcgggc
      121 cgcctatgaa aacatagttg atgatccaga ctgcatcgct gacattgata gtagcatcgc
      181 agttggcgtc acctttctcc ttgcaggagg gaatatcgcc tcccacgaag acatagttga
      241 taatatacac gccatcacta acattagcat ttccgtcgtt attggcgtcg ccgggagtgt
      301 cgcagcatga tgccgcaaaa gcattgctct gcgcaaggaa actgccagcc agcagcagaa
      361 caccaagcgt cagaatgaat tgcaggtgtt tcatgaaatc ctccgttttt aaatattttt
      421 aaattacttt atatgcagtt aatcagactt tttcgaacag acgtaattcc gaaccggcaa
      481 gcagccggca tcaagtgggt acgaaattcc gatccggcag taaattctaa tattttcacc
      541 ccatttatac tttacaccgc catatcacct atggaagtaa atattgtcaa catatactgt
      601 gtttggatca cccgagatta tcagttgggc cagatgtgcc ctggtagtaa gattaacaaa
      661 gtcggtaaac ggtatttcaa tgctgaccca gctaccggta ttcatggtgt tttgatccag
      721 ggtgatttcg tgctcgacat catcaccgcc atcatcccag acaccgttgg cgccaaaatc
      781 taccagcttg attctaaaca cagcgggagc atccgtggga tcgggagtcc agagatccat
      841 gtggaaatgg gtcatcgcag aagcatcgat tgtattggtt gtaaactcta tgcctgcaaa
      901 caacaggttg gtgtattttt tgacggtatc agaaccgatc atatagtttt gaacatcggc
      961 agtatcccag acagccgacc aggtatcaac cggtacgtcg gtataggcgt cgctgaaaag
     1021 agagatcaca tcgccagcat cagttgcagg tgtcggtgcc gcaaccggcg gagcttccgg
     1081 aataccggaa tcatagaaat aaatattatc gacaaataca gtgttcggat cacccgagat
     1141 gattaactgg cccagatgtc ctcttgtggt taagtcgcta aactcggaca gagaaaaatc
     1201 tatggttacc cagatgccag tgttcatgat gctttcaccc aaagtaattt catcttcgac
     1261 atcgtcgccg ctgccaccct cgtaaacacc attggctcca aaatcgacca gcttgatctt
     1321 gaatactgac ggtgaagatg tcgcatccgg ggtccagaca ttcatatgga aatgagtcat
     1381 cgcggaagca tcaactgtag aagttctgaa ttctatgccg gcaaagagga ggtcagtgta
     1441 ttttttcatg ttgtcagaac cgatcataaa gtcttcgaca ttagcgacat cccagaccgc
     1501 cgaccatgtg tcaacggcca cgttggaata gacgtcactg aagagagata tcaccaagcc
     1561 cgggtcctcg tccggcgccg gggcgggtat attcggtgcc gtctgctgac cggcatcgta
     1621 gaaatagata ttgtcaatat agacggttga aagatcaccc gagatgatca gttgccccag
     1681 gtgccccctg gttatcaaac cggagaatgc cgccagcggc agatcaatgg aaacccaggc
     1741 acccgtagca agggccgggg aggtgttttc atcaaagacc agctcgtgtt caaaatcatt
     1801 tccgccgccg aaaacaccgt ctgccccaaa atcaaccagc ttgattttga acttggcggg
     1861 tgacgcagtc gaattgggcg tccagatgtc catatggaag cgggtcatgc ccgagacatc
     1921 tatcgtcgga ttcgcgaatt cgattccagc gtattgcatg ttttcatact tcttaacatc
     1981 gtcactgcct atcgtttcat cgcttacatc cgccatgtcc cagtcggccg accaggtgtc
     2041 caccgtaaca tcagtgtagg cgttgctgaa caaagaaatc gcgtcggctt caggaactgt
     2101 cggtgtggga gccggagtgg taggggtagc agcctgtccg gccacttcca cagtcactgt
     2161 cccggaggcc tggactgttc ccagagtagc ggtcagctcg gaggttccca ctccagccgc
     2221 ggtgatggta ccgttgtcag caacattgac tacagtctca ttcgaggaca cgaatgtgaa
     2281 gtatccctgc attgccgaga ccgtctgaga actgccgccc acatcaaaag taaccgtagc
     2341 ttccgtgata gtgatcgtgt cgctcacctc agcttcgatt gttctggtgg gaatagctgg
     2401 ctgaggattg gagatcgtgc caagtgtctc aaaaataatt tcgtcaaacc agagctgata
     2461 tccgcttccg ttctccggtc cttcagcaaa atagaaaagt cccttttccg tcgttagttt
     2521 atcggaaagc ggaatcggta taatatattt ttgccattct gtggtcagcg gcaaattatt
     2581 tacttcggcg gtgaattttg aagttccggt gttatcattg ccgattccgg ccacattaag
     2641 agttgcgttc atgctggcct tggcgtaaaa cgtcaaagcg ttatattggg tcaaatcacg
     2701 agcaatgcca gcggtaaaag cgccgccggc gtagcttccc gaaggatcgc cgacattggg
     2761 gactgttacc tgtaaagctt gagaaccggt gtaggtattg acctgatcta aatcgaccgc
     2821 atcaagtttg gaaccggcaa atgcctgaaa agtgacgctg cttccgaatt catccctgaa
     2881 gacaaccgcg tcagtcggaa actgcacggg ctccagaacc ctgtcatcat tgcagccaac
     2941 caatccgaca atgaccatta tcgaaatgaa ggccccaaga tgaatcttgc ctctggtgaa
     3001 caactatta
//
LOCUS       DSEB01000217            2209 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1008114, whole genome shotgun sequence.
ACCESSION   DSEB01000217 DSEB01000000
VERSION     DSEB01000217.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1008114"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            1009..>2209
                     /locus_tag="ENO07_03910"
     CDS             1009..>2209
                     /locus_tag="ENO07_03910"
                     /inference="COORDINATES: protein motif:HMM:PF02074.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HDS01143.1"
                     /translation="MAEDAKKLYEELIEKRRKAALLGSAGSILGWDERTYMPRGGVEH
                     RANQMALISGMAHEMFTSPEIGELLSKLEDSELVKERTSPEAANIREIRRSYNKDTKL
                     PRELVEEISSTTTKAQGIWQDARKKADFSIFKDILGKVFELNFRVAEAYGYEKDPYDA
                     LLDDYEPHATTEEVARVFAGLRDDLVPFVEKIKDSGKHPDMSIIENDYDIHRQKLFAK
                     AASAAIGFDYSKGRLDETTHPFCTGIGYGDTRILTRYNPKHFGQAFFGVIHESGHGIY
                     EQGLRKDENFGLPMGQSVSLGIHESQSLCWENIVSRGKPFWKHFFPRAQQTYPEILGD
                     VKFDDFYFAINDVRPSFIRVDADEVTYCLHILLRFEIEQGIIHKEIKVADIPGIWNEK
                     FTEYFGIT"
BASE COUNT          601 a          516 c          564 g          528 t
ORIGIN      
        1 gagggcaccc gatactatta tcagctgggt gccgccgatt gaaatcggag acaccctgac
       61 cgctgagatt ccaatcaaat tcaatggggt gggtgaattc atcgtgcaac tcctggagat
      121 ggacaatacc aaggatatga tcaggatgat ggtctcggtc gtgatcgacg agaaaggcga
      181 acttcattat ctgggccaat atccgccgcc tgataaaaat ccgctaaagg cacatcacta
      241 tgtattcggc ggggaaatcc gtgcccatgt tgaaggcagg gcagtaagaa ccgggataca
      301 aagacaaacc tggccggaac catttgatat tgatatgaca ttctcccctt cacctgaggt
      361 cggcaaaaga tccaaagtcg agtttacaat atcgccgctg atttcagatg ttgccgatct
      421 gcaataccag attatcgggg cgactaatct tcaaatcgat tccctctctc cttctccggg
      481 aacaatgcct gaaaatatca acagattcga ggcctctttc cagatgattc ctcagaagac
      541 cgggagatca tacatctcat ttgaaatttt cgggcataga ccggacgcgc tgcacggcag
      601 taggccattt tccagaatag actatcattt agtgtttgga agtgacagca gccttcttta
      661 tatgggtgaa gtcgatccat ttgctgttgg tttcgagact ggcaatccgg cttatgaggc
      721 aattgacgag atcgcagatt tctctgaaac cctctacggc gaaaaagttt accgatcaga
      781 gccggacttt gagtatgacc gcataaatca gcagcggatc atagattcga ttgaagccag
      841 caagctgatc gatactctgg ataccgggga ccaggaataa cccgcaataa cgccgtcatc
      901 acatcagcag ggcggttttc cttgaaaaac acagcttctt ttgttacatt cgtaaattaa
      961 acttagtgct acaggtgcga gtatttaaaa aaaggaaagg atagattcat ggcagaagat
     1021 gcaaagaagc tgtatgagga gctgatagaa aaaaggcgca aagctgcgct tttgggttcg
     1081 gccggaagta ttctgggatg ggatgagcgc acatatatgc cgcgcggagg tgtggaacat
     1141 cgcgccaatc agatggctct catttccggc atggcgcacg agatgttcac ctctcccgag
     1201 atcggggaat tgctttcgaa gctggaggat tccgaattgg tcaaggagcg gacttccccg
     1261 gaagcggcaa atatcaggga gatcagacgt tcttacaaca aagacacaaa gctgcccaga
     1321 gaactggtgg aggagatttc ctcgaccacc acgaaagctc agggaatctg gcaggatgcc
     1381 cgcaagaaag cagatttcag cattttcaaa gacattctcg gtaaagtctt cgagctgaat
     1441 ttccgcgtgg cggaagccta cggctacgaa aaagacccat atgacgcgct tctggatgat
     1501 tacgagcctc atgccaccac tgaagaagtg gcaagggttt ttgcgggact gcgtgatgat
     1561 ctggtaccgt ttgtggagaa gatcaaggat tcgggcaaac atccggacat gtccattatc
     1621 gaaaacgact acgacattca tcgccagaaa ttatttgcca aagcggcctc ggcggcaatc
     1681 ggattcgact attccaaagg ccgtctggac gaaaccactc atccattctg caccggaatc
     1741 ggatatggcg ataccaggat tctgacccgc tacaatccca aacatttcgg ccaagctttc
     1801 ttcggtgtga tccatgaatc ggggcatggc atctatgaac agggcctgcg caaggacgag
     1861 aatttcgggc ttccgatggg ccagtcggtt tcactcggaa ttcatgaatc gcagtcattg
     1921 tgctgggaga atattgtcag ccgcggcaaa ccgttctgga agcatttctt cccgcgcgcc
     1981 cagcagacat atcccgagat tctaggcgat gtgaaattcg atgatttcta ttttgcgatc
     2041 aatgacgtac gtccttcatt cattcgtgtc gatgcagatg aggtgacata ctgcctgcat
     2101 attctccttc gattcgagat cgagcagggt attattcaca aggagatcaa ggttgcggat
     2161 attcccggta tctggaacga gaagtttacc gagtattttg gtatcactc
//
LOCUS       DSEB01000218            2514 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1006107, whole genome shotgun sequence.
ACCESSION   DSEB01000218 DSEB01000000
VERSION     DSEB01000218.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1006107"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1010
                     /locus_tag="ENO07_03915"
     CDS             <1..1010
                     /locus_tag="ENO07_03915"
                     /inference="COORDINATES: protein motif:HMM:PF07228.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDS01144.1"
                     /translation="FLSAVREMYPDLPRILLTGYADKENAIKAINEVSLFQYLEKPWD
                     NEQLKLVLKNAIESKSLREHLHMKIKELDEVLRHRDELFEHNNMIARELKLARKLQQA
                     LLPRKLPQIDGVKFETYCKPAIEIGGDFYDIIPMQDGCWSVLVADATGHGIQAALSTA
                     LLKFAFVSFAGGNHQPSQILSGMNEILEKGLPEELYVAALVARIDPGKREITISNGGL
                     TYPYLIRKQENKSELVASNGMMLGFIRPEMYKPGPDKAVMLDPGNSLILYTDGLTEAV
                     NKDGDFFESKNLPEVLKKLGSSACGEIINGLAESLGDFNGGVYSDDVTLFGIEINTDR
                     R"
     gene            1013..1495
                     /locus_tag="ENO07_03920"
     CDS             1013..1495
                     /locus_tag="ENO07_03920"
                     /inference="COORDINATES: protein motif:HMM:PF00072.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDS01145.1"
                     /translation="MVDDKEKSTVVIVDDEEMVLTSLNSFLSLETEYTVETFTSAKDA
                     LEYIKKNEIDVVISDYLMPEMDGISFLAKVKEMRPQVPRIILTGYADKENAIKAINDV
                     GLFQYIEKPWDNDDLLIVLRNGIERQKLMKKLEEKIAEINKAYGELQGLQTEIVKTFI
                     "
     gene            complement(1492..2343)
                     /locus_tag="ENO07_03925"
     CDS             complement(1492..2343)
                     /locus_tag="ENO07_03925"
                     /inference="COORDINATES: protein motif:HMM:PF01569.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDS01146.1"
                     /translation="MKKVGSYKILLILSILFFSFRPLPADDREYLSSTEITGISAGSI
                     ALGAAGLWALDFDSTRRPVISGPAPWEESIQRFLGGRYYPGKTNWLDHSFGSSITPIL
                     MGGLLLGADISWPRYDRAKDVWQDMFLYGAGLLATKGVTGITKGFFARPRPMVALEPE
                     LYNRNKNNDYAQDRASFFSGHTSSAFFSTAFLNMRIRQIMRQEMTYNDYKDWRWLPPT
                     ILFGWASYVGYTRIDAYKHYITDVVFGALAGWVMAELFYHFGDNIEKPRDSSSRGKVL
                     FKIGFSF"
BASE COUNT          692 a          565 c          685 g          572 t
ORIGIN      
        1 agtttttgag tgcagtgcgg gaaatgtatc ccgacctgcc ccggattctt cttaccggtt
       61 atgccgacaa ggaaaatgcc atcaaggcca tcaacgaggt aagcctgttc cagtacctgg
      121 aaaaaccatg ggacaatgaa cagcttaagc tggttctcaa aaatgctatt gaatccaaga
      181 gcctgagaga gcatctgcac atgaagatca aggagctgga tgaagtcctg agacatcgtg
      241 atgagctctt tgaacacaac aatatgattg cacgtgagct gaagcttgcc cgcaagctcc
      301 agcaggcatt gctgccgcgt aaacttcccc aaattgacgg cgtgaaattt gaaacttact
      361 gtaagccggc gatagagatt ggcggtgatt tttatgatat cataccgatg caggacggct
      421 gctggtcggt attggtggcg gatgccaccg ggcatggcat acaggcggct ctgagcaccg
      481 cgcttctgaa attcgccttc gtatcatttg ccggaggaaa ccatcaaccc tcgcaaatcc
      541 tgagcggcat gaacgaaatc ctggagaagg gcctcccgga ggaactctat gtggcggcac
      601 tggttgccag gatcgatccg ggcaaaaggg agatcaccat atctaacggc ggcttgactt
      661 atccgtatct tattcgcaag caggaaaaca aatcggaact ggttgcgtct aacggtatga
      721 tgctgggctt tataagaccg gaaatgtata agccggggcc ggataaagca gtaatgcttg
      781 acccgggcaa cagtttgata ttatatactg acggactgac cgaggcggtc aacaaggatg
      841 gagacttttt tgaaagcaaa aatctgcccg aagtcttgaa gaaattaggc agctccgcct
      901 gcggggaaat aattaatggc ctggcggagt ccctgggtga ttttaatggc ggggtgtata
      961 gtgatgatgt tacactgttc ggaatcgaaa ttaacacaga caggagatag agatggttga
     1021 tgataaggag aaaagcacgg tcgtaatcgt tgatgatgaa gagatggtcc tgacgagcct
     1081 caattcattc ttgagcctcg agacggaata cactgtcgag accttcacct cagcgaaaga
     1141 cgcgctggaa tatatcaaga agaatgagat tgatgtggtg atttccgatt atctgatgcc
     1201 ggagatggat gggatatcct ttctggcaaa ggtcaaggag atgcggcccc aggtgcctcg
     1261 tattattctg accggttatg ccgacaagga aaatgcaatc aaggctataa atgacgtcgg
     1321 actgttccag tatattgaga aaccgtggga taatgatgat ctcctgatcg ttcttcggaa
     1381 tggcatcgaa agacaaaagc tcatgaaaaa gctggaggag aagatagcgg aaatcaataa
     1441 ggcctatggt gagcttcagg gtttgcagac cgagatcgtg aagacattta tctaaaagct
     1501 gaatcctatt ttgaatagaa ccttgcccct tgatgatgag tcccggggct tttctatatt
     1561 gtcgccgaaa tgataaaaca gctccgccat cacccatcct gccagagctc caaaaacaac
     1621 atcggttata tagtgtttat aagcatcgat acgggtgtag cccacgtagg aggcccagcc
     1681 gaacaggatt gtcggcggca gccagcgcca gtccttgtag tcattatagg tcatctcctg
     1741 gcgcatgatc tggcggatgc gcatattcag aaaagcggtg gagaaaaagg cgctggaggt
     1801 atgacccgag aagaatgaag cccgatcctg ggcgtaatca ttatttttat tccggttgta
     1861 aagctcaggc tcaagcgcga ccatgggacg cggacgtgcg aagaaaccct tggtgatccc
     1921 ggtaacgcct ttggttgcaa gaaggcccgc gccgtacaaa aacatatcct gccagacatc
     1981 tttggcgcga tcataccggg gccagctgat atcggcgccc agaagcaaac cgcccatcaa
     2041 aatgggagtt atggaagagc cgaagctgtg gtccagccag ttggttttgc ccggatagta
     2101 tctcccgccc agaaaacgct gaatcgactc ttcccatggc gccgggcctg aaattaccgg
     2161 acgacgggtg ctatcgaaat cgagtgccca cagccccgcg gctcccagcg caatcgagcc
     2221 ggccgatatg ccagtgatct ctgtgctgga gagatattca cggtcgtctg ccggtaaagg
     2281 ccggaaggag aaaaagagta tggatagaat caataatatc ttgtaactgc cgactttttt
     2341 catatccgct taccgctcaa gccatcattg gagtttactt atgagaatct tatcgcaggg
     2401 aatttgcagg ctgtcaaggg ccttgaaaaa atgacccagc gtggtcaggt aatggtcgga
     2461 acgttcggag atatcctcgc ggatagtcgg ggaaacattg ctgtagaaat aatg
//
LOCUS       DSEB01000219            1758 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1013464, whole genome shotgun sequence.
ACCESSION   DSEB01000219 DSEB01000000
VERSION     DSEB01000219.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1013464"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            27..623
                     /locus_tag="ENO07_03930"
     CDS             27..623
                     /locus_tag="ENO07_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01147.1"
                     /translation="MSIETLIAAVLTLAIFSFLYKENPFYRAAEHLLVGVSVGYYLVI
                     LVEETIWSKVARPLAYDQNWIVIIPAVLGLMMFLRLSRNRAYLTRIPLALLIGAGAGV
                     SIPAMLWARVLKQMSASMLPLYTVSGMIVLVGVITTIIYFYFSREQEGWFGYLAKTGT
                     YFLMVFFGATFGYTVMSRVTLLIGRMEFLLTDFLGVVG"
     gene            785..1582
                     /locus_tag="ENO07_03935"
     CDS             785..1582
                     /locus_tag="ENO07_03935"
                     /inference="COORDINATES: protein motif:HMM:PF01904.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDS01148.1"
                     /translation="MPKNFNTKIEIGTSGYSFIDWIGPFYPAGIQKGKMLDYYVQHFS
                     TVEINSSYYRIPHPRVFEAMIKKVPEDFEFTIKAHKSLTHERRDIMEPTARFREALLP
                     FKGTNLLSGILLQFPYSFKYSAENLNYVRRAGDLLIGQDVFVEFRHKGWIRDEVFQAF
                     ESEGISFVSVDEPSLEGLIKPEAYTTSDTGYIRLHGRNSRTWWSGGSERYDYLYNERE
                     LSEWSKKIEEMKKKTRKIYIFFNNCHHGQAVQNAKMMLDLLKSEKGE"
BASE COUNT          454 a          384 c          455 g          465 t
ORIGIN      
        1 atctcggcct atgtctggag aaagaaatga gtattgaaac gctgattgcg gcggtactga
       61 cactggctat attctccttt ttgtacaagg agaacccgtt ctatcgcgcc gcggaacatc
      121 tgcttgtcgg tgtctcggtc ggatattatc tggtaattct tgtggaggag acgatctggt
      181 ccaaagtggc cagaccatta gcctatgatc agaactggat tgttataatt ccggctgttc
      241 tggggctgat gatgttcctc cgattatcac gcaatagggc ttacctgaca cgaataccat
      301 tggcactgtt gatcggcgcc ggggcgggtg tctcaattcc agccatgctc tgggcgcggg
      361 tcttgaagca gatgtcggcc tccatgctgc cgttatatac tgtcagcgga atgattgttc
      421 ttgttggagt tatcaccaca atcatctact tttacttctc gcgcgagcag gagggctggt
      481 tcggctacct ggcaaaaaca ggcacctatt ttcttatggt tttcttcggg gcgactttcg
      541 gttataccgt tatgtcccgc gtgacccttt tgatcgggcg gatggaattt ctgctgaccg
      601 atttcctcgg ggtcgtggga tgatctgccc tcgctgcagc gctcccatga aagagctgaa
      661 acgtacgtat cataaacgga gaaaatgggt ttgtcccaaa tgcaaatatg tccgctttca
      721 ggatatctcc aaaagaaaat gacatttttg ccgattaaat taacagcatg ggtttcattt
      781 tggtatgccg aaaaatttca atacgaaaat agagatagga acttccggct actcatttat
      841 tgactggatt ggcccttttt atcctgcggg gattcagaaa ggcaaaatgc tggactatta
      901 tgttcaacat ttcagcacgg ttgaaatcaa ttcgtcgtat taccgcatac cgcatcccag
      961 ggttttcgag gccatgatca aaaaagtacc ggaggatttt gagttcacga tcaaggcaca
     1021 caaaagcctg actcatgaga gaagagatat tatggaaccg acagcccgct tcagggaggc
     1081 actgctgccc ttcaagggga ccaatctgct ttcaggcatc ctcctgcagt ttccatactc
     1141 attcaaatat tcggccgaga atctgaacta tgtccggcgt gccggcgatc ttctgattgg
     1201 acaggatgtt ttcgtggaat tcaggcataa gggatggatt cgggacgaag tttttcaggc
     1261 ttttgaaagc gagggaatct cattcgtcag tgtcgatgaa ccctcgctgg aaggtttgat
     1321 caagccggaa gcatacacca cttcggatac cggatatatc cgcctgcatg gacgcaacag
     1381 ccggacctgg tggtcagggg gctcggaacg ttacgattat ctgtacaatg aacgtgaatt
     1441 aagtgaatgg tcgaaaaaga tcgaggagat gaagaagaaa acccgcaaga tttatatatt
     1501 cttcaacaac tgccaccatg ggcaggcagt gcagaatgca aagatgatgc tggatctgct
     1561 gaaatcggaa aagggagaat gaggatgaga aaaaaggtaa tgggattttt ggtcataggg
     1621 gtgatagctt atatcggcct tggatttatg gaggggtcgg gtagtgttgc tgtaaattac
     1681 atcagtactg gcacaaccat cgacacaata aactcacttc cgccggtcga actcgtaaag
     1741 accgacatgc acctgacc
//
LOCUS       DSEB01000220            2015 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1009907, whole genome shotgun sequence.
ACCESSION   DSEB01000220 DSEB01000000
VERSION     DSEB01000220.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1009907"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            41..1411
                     /locus_tag="ENO07_03940"
     CDS             41..1411
                     /locus_tag="ENO07_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01149.1"
                     /translation="MIEFMRFAYTIGSRTLFKEIKRLRPGEALRYQAENGQIRFDDQS
                     PLWTEEYSGSKKRLVEEGCELLIDACKTMANSMLMMSAGWDSRTLLAGAFAAGVGKTV
                     KFYHHGDLRSRETEMVKAIAQKTGNELILRRIETHFFDSEFLEKCFSEYNNVLFPTWH
                     VAGETSRQDSFQIDSVCAGIPGEILGGHFGPPRTMHGLRKVASTFYYIADLPGFIDPR
                     KRLKQTNPSEAIMLLHQPEYPTPWYFNADLWAERFVRIDKAVNNDIEQEIKRYITRGI
                     ENRDSAFEAFFSERSSQYIAGQVLSSATHKPLVAPYCNRDFITFASCVSFRDKANNSL
                     NRGIVKRLCPDLLQFPTAAILCKANRPVILQEVSRVHRKAIQKWQNAKHKISKGKSEI
                     PIWGWANFGCLDSPNAYDKLIESFESPLWDKAKIKNSIPNINAPSFGIMGMLLRMKTL
                     DLFGVK"
BASE COUNT          555 a          456 c          512 g          492 t
ORIGIN      
        1 actcaattca gtctgtaact gcgcaacaga cgacattggt gtgattgaat tcatgcgttt
       61 cgcctatacg ataggttcac gtactttgtt taaagagatt aaacggctca gacccggaga
      121 ggcgcttcga taccaggcgg agaacggaca aatcaggttc gatgatcaaa gccctctctg
      181 gacagaagaa tattcgggga gcaaaaaaag gctggttgaa gaaggatgcg aacttctcat
      241 tgatgcctgc aagacgatgg ccaatagtat gctgatgatg tcggctggct gggattcacg
      301 aactcttctt gcaggcgcct tcgctgctgg cgtaggaaaa actgtaaaat tctatcatca
      361 cggggatttg agaagccggg agactgagat ggtaaaggcg atcgcgcaga aaaccggcaa
      421 tgaacttatc ctgagacgaa tagaaactca cttcttcgac tctgaattcc tggagaaatg
      481 cttttcagaa tacaataacg tactatttcc cacctggcat gtcgccgggg aaacatccag
      541 gcaggattca ttccaaattg attcagtatg tgccggaatc cctggggaaa ttcttggcgg
      601 ccattttggt ccaccccgca ccatgcacgg tttaagaaag gtagcttcca cgttttatta
      661 tattgccgat ttaccgggct ttattgatcc gcgcaaaagg cttaaacaaa ccaaccccag
      721 tgaagctata atgctgctcc atcaaccgga ataccccaca ccgtggtatt tcaatgcgga
      781 tctctgggct gagagattcg tgaggattga caaagcggta aataatgaca tcgagcagga
      841 aatcaaaaga tatataacgc gggggattga aaatagagac tctgcatttg aagccttttt
      901 ttcagaacgg agcagtcaat acatagcagg gcaggtactg agttctgcaa cacataaacc
      961 tctcgtcgcg ccatactgca atcgggattt tatcaccttt gcgtcttgtg tttcatttcg
     1021 tgacaaagct aataactcac tgaatcgtgg gatcgttaaa cgtttatgtc cggatttgct
     1081 tcaatttcca actgcagcga ttttatgcaa agcgaataga cctgttatac ttcaggaggt
     1141 ttcgcgagtc cacagaaaag caattcaaaa gtggcaaaat gcaaagcaca aaatttcaaa
     1201 ggggaaatcg gaaataccga tatggggctg ggcgaatttt gggtgtctcg acagcccaaa
     1261 tgcttatgat aaattgatcg aaagttttga atcgccgctg tgggacaaag caaagataaa
     1321 gaactccatc ccgaacataa atgcgcccag ctttggtatc atgggcatgc ttctcagaat
     1381 gaagaccctc gacttgttcg gggtcaaatg agatcacaag ccatagctac tttaaaccga
     1441 ccgcagttga tatattttaa ggctcggaga cgggccggaa cgagttattt aatacccgtc
     1501 taaatcgcca acatcgatgg gcggagtacc gtcaatctta taagacacag acaccgtttg
     1561 gggactgtta tcggcattac tgctgctgat cgtgattgca gcgttatatt gaccgaccgg
     1621 aagatctgta tcattgatgt acacagatac gacctggctg ttgctgctgc cggacacagg
     1681 atcgacagac aaccagcctg cgttatcgga aatactccac tccagcgtgc cggcgcccgc
     1741 attcgtgatg ctgaaggatt ttgactcggg cggggtgccg ttcggctgcg cggcgaaagc
     1801 caggctgttt ggacttacag atatatgcgc cgaagaaaat ggattgaacg cgcccatgta
     1861 ggtcggatat gcccctcccc gtccggaagt agccgcgggt gaggtggtac tgatcttcag
     1921 aaaatcagcg ctttgcggat tggtgctcag gaactgcggg tcggcccaga tggaattatt
     1981 gtcgatgcca tgcgcgctga cgtgctccgc taagg
//
LOCUS       DSEB01000221            2872 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004528, whole genome shotgun sequence.
ACCESSION   DSEB01000221 DSEB01000000
VERSION     DSEB01000221.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004528"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(123..743)
                     /locus_tag="ENO07_03945"
     CDS             complement(123..743)
                     /locus_tag="ENO07_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01150.1"
                     /translation="MLSNICKRGFPEDRLDKIPEPKIKRDENGWYIVSISEGVKVYIE
                     KYYDFLEKLWDRAAPLYQEVENKLADTPEDSMEAKAFYRSRKLILEIVLGTIKKFYTA
                     DDSLGVFMSPWCFGTVVLEKVEQVRDMIKKGEPVKHDVGDYPYYVVRYIDETHRKALL
                     DLFSFPEKAFSMRWQYSELLKRYSKTLTNITTSLQSVLLMVRSYTS"
     gene            972..1439
                     /gene="ruvX"
                     /locus_tag="ENO07_03950"
     CDS             972..1439
                     /gene="ruvX"
                     /locus_tag="ENO07_03950"
                     /inference="COORDINATES: protein motif:HMM:PF03652.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDS01151.1"
                     /translation="MLTVLMLHMANGLRIYLRMRIMAIDFGTKKIGLAFTDPTLSFVS
                     RTLTLRVKSEKEAAQKAVEFVSSEDVHKIILGYPLNDDGTKSEMTHRVEDFRKRLEQL
                     VDIEIEYSDERYTSEEAEKYLHQMGKSIKGNKNRIDAISAALILNDYLGEQRR"
     gene            1560..2552
                     /gene="mltG"
                     /locus_tag="ENO07_03955"
     CDS             1560..2552
                     /gene="mltG"
                     /locus_tag="ENO07_03955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDS01152.1"
                     /translation="MKRILLIILVIALGMLVYFGLYLGGDANPTNGNIYLMVHPGDGA
                     GTVLHRADSLDLKLSKTAAKLYLRIYGAERRIVPGRYTVEPDDSRLDLLKRIRNGEID
                     YVWITVPEGLTVNRTIEIMADRSGRTAQEFLLLANDPEFLSSLPFSPEALEGYLFPET
                     YKAPYHAEPDYLINIMVNALYEFLDSRLMARAEEIGLDVKELLTLASLIEAETAAREE
                     MPVISSVFHNRLKRDMLLQCDPTVIYALGGLDRPLYTKDLKVDSPYNTYLYKGLPPGP
                     INSPGRDAILAALYPDSTNYLFFVADISGRHVFSATNAEHEKARREIKRKRRLE"
BASE COUNT          799 a          637 c          687 g          749 t
ORIGIN      
        1 agcctgcgct gcggcccgct tcggcgaggc cccgaggtga tatcagacgc cataatgtgc
       61 atatattata aaagaatccc gtcaagctga cgggattctt tttgaaaggc gggtagagta
      121 tttcaagagg tgtaacttct caccatcagc aagaccgact ggagagaggt tgtaatattg
      181 gtcagggttt ttgagtaacg tttcaaaagt tccgaatact gccagcgcat ggaaaaagct
      241 ttctccggaa agctgaaaag atccagaaga gcttttcgat gggtctcgtc gatgtatctg
      301 acaacgtaat aagggtaatc accgacatca tgctttacag gttcgccctt cttgatcata
      361 tcgcgaacct gttccacttt ttccagcacc actgtgccga aacaccaggg cgacatgaaa
      421 actcccaggc tgtcatcggc cgtatagaat ttttttatgg ttcccaggac aatttcgaga
      481 atcaatttgc ggctcctgta aaaagccttg gcttccatgg aatcttcagg agtgtcagcc
      541 agcttattct caacttcctg gtagagcggt gcagctcggt cccagagctt ttccagaaag
      601 tcgtagtatt tttctatata aaccttaacc ccctctgaga tgctgactat ataccagcca
      661 ttttcatctc gttttatctt gggttccgga atcttgtcca gtcggtcttc aggaaaacct
      721 ctcttacata tgttgctcag cattgctgac ctccttaaac gaccttttgt tctaatcctt
      781 atttcagcaa cagttgtgcc atcgtcctgg atttgcggag gccaaaatcg aggagcccgg
      841 cacaccgtcg cattccttta aatagcaata agatggatgg attatgtaag ctcctgcagc
      901 gggtcagtag cgggctgttt ccagacgtat agcattactt tgcatttatc agtatgcatt
      961 aaaatgcata aatgttgact gttctgatgt tgcacatggc caatggattg cgtatatatt
     1021 tgcgtatgag aataatggca attgatttcg gcacgaaaaa gatcggcctg gcatttacag
     1081 atccgacact tagtttcgtt tccaggacat tgacattaag ggttaaatca gaaaaagagg
     1141 cggcccagaa ggcggtcgaa tttgtttctt cggaggacgt tcacaagatt attctgggat
     1201 atccgctaaa tgatgatggc acaaagagcg agatgaccca cagagtggaa gatttcagaa
     1261 agcgcctgga gcagcttgtc gatatagaaa tcgaatattc ggatgaacgt tacacctccg
     1321 aagaggcgga aaaatatctg catcagatgg gtaaaagtat taagggaaac aagaacagga
     1381 tcgatgctat aagtgcggcc ctgattctga acgattacct cggggaacag aggcgatgat
     1441 ttaatgctga gagcggtttt ctcaatttta gtttctgtaa taataatgct tgcggcagtt
     1501 ctttattttc ctatgagctg gctggcagac atttttgcgc acctcgggag agcgaaaact
     1561 tgaaaagaat tttgctgatt atcctggtta ttgcactggg aatgctggtt tatttcgggc
     1621 tttacctagg gggcgacgct aatcccacca atggaaatat ttatctaatg gttcatccgg
     1681 gagatggtgc gggcaccgta ctgcatcgcg ccgacagcct tgatctcaag ctctcaaaaa
     1741 cagcggcgaa actttacttg agaatctatg gcgcggagag aaggattgtc ccgggcaggt
     1801 acactgtcga accggatgac tcgcgtctgg acctgttgaa acgaattcgt aacggagaaa
     1861 tcgattatgt ctggatcaca gttccggaag gcctgaccgt taatcgtacg atcgagatta
     1921 tggccgaccg ctccggaagg acagcacagg agtttttatt gctggccaat gatcccgagt
     1981 tcctgagttc gctgcctttt tctccggagg ccctggaggg ttatctgttt ccggagacat
     2041 acaaagcccc atatcacgcc gagccggatt acctgatcaa tattatggtt aatgcgctgt
     2101 atgaatttct ggacagccgg cttatggccc gtgcggagga gatcggtctt gatgtaaagg
     2161 agcttttgac cctggcatca ttgatagaag cagagacagc cgccagagag gagatgccgg
     2221 ttatatcgtc ggtctttcac aaccggctga aaagggacat gctgcttcaa tgtgatccga
     2281 cagttatcta tgcgctgggc ggactggatc gtcctctcta cacaaaggat ttgaaagtgg
     2341 attcccctta caatacatat ttatataaag gtctcccgcc cgggccgatt aactctcccg
     2401 ggcgcgatgc gattctggca gccctctatc ccgacagcac caactactta ttctttgtag
     2461 ctgacatcag cggcaggcat gtcttcagcg ctaccaatgc agagcacgag aaagcaagac
     2521 gcgaaataaa aagaaagcgg cgccttgaat aattaaagtc ctgctctcaa tcctctctat
     2581 ttttgcgcac aaacttccgg gataaatgat ggactctatt ctaaaataaa tttttgcagc
     2641 aatagttttt gttgactata tgttaactaa aaacctaact tgtcgaaaat tagaattaga
     2701 taacaacctt tttattagga ggagggtatt caatgcctgc caaaaagaaa gccgcaaaga
     2761 aaaaaacagc cacgagaaaa aaagctgcca agaaaaaagt agccaagaga aaacccgcgg
     2821 ctaagaagaa aactgctgcc aagaaaaaac cggccaaaaa gaaagctgca gc
//
LOCUS       DSEB01000222            1804 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1012709, whole genome shotgun sequence.
ACCESSION   DSEB01000222 DSEB01000000
VERSION     DSEB01000222.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1012709"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..278)
                     /locus_tag="ENO07_03960"
     CDS             complement(<1..278)
                     /locus_tag="ENO07_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01153.1"
                     /translation="MKKGNETIYVFLVAILLIPLSLFASRDFEPVFKPDLAIAKLTES
                     IKIDGKLDDRQWAMASQITQFHERHPRELAEPEVSTRALLAYDEDNLY"
     gene            749..>1804
                     /locus_tag="ENO07_03965"
     CDS             749..>1804
                     /locus_tag="ENO07_03965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HDS01154.1"
                     /translation="MAGINDHKTTGLKKQPGYIFDIDDIPHLSDREKARLKEVTRFFA
                     FRSNEYYLRLIDWDNPNDPIRRIIIPDEDELNDWGEMDACNERSVTVRKGVQHKYGST
                     VLLLVHEVCNSFCRYCFRKRLFINGGNEVSYNVDEGIEYIKKHKEVNNVLLTGGDPLV
                     LPTRRLEKIIKALREIEHVRIIRLGTKMTAFNPYRFINDPELLEVLSRYSEPDRKIYV
                     VCHFDHPREITPQARQAVHLLQKAGCILTNQNPIIRGISDKPSVMAELWNELSYMGMP
                     QYYIFQCRPTAGNEPYAVPITEAYFKIEEAKRRCSGLAKRLKYVMSHETGKIEVVGVD
                     DNFIYMRYHRAKLEDDEQ"
BASE COUNT          493 a          408 c          439 g          464 t
ORIGIN      
        1 atatagaggt tgtcttcgtc ataggccagc agggcccgtg ttgaaacctc cggttccgcc
       61 agctcccggg gatgcctctc gtgaaactgg gttatctgcg atgccatcgc ccattgccgg
      121 tcgtcaagct ttccgtcgat cttaattgat tcagtcagtt tggcaattgc aaggtcaggc
      181 ttgaaaacag gctcaaagtc ccttgaagca aaaagagata atggaattaa tagaatggcc
      241 accagaaata cgtaaatggt ttcgttacct tttttcataa acgtctcctg gttatggtaa
      301 tcattacgtc atttttcacg attggttgcg ggaatggatc aaaacaggaa taaagtatgc
      361 cgttggagtc catttatttt caaaaaatat tgctttgagg ggcaagaagt taccatagca
      421 tgtgaatgat tttacacata gaaaaaccct catgtgaaat taatcacatg actcctaaat
      481 ataattcaaa gatctatttg aacttagacg tattttattg gcataaaata ttgacagatt
      541 aaccgtaacc tgttatatta ttacttaaag gggatcggag ttttggcttc aatggatttg
      601 ccatattata tgacttcgac cgaatcacgc ggatcgcttt cctgattgag acgcttttgt
      661 atccgcacga gagcttcaat gcaggctcgc ttttacaacc aggtgacatc gcgattgatg
      721 ttgctacctt tttgggaaag gaggttttat ggccggaata aacgatcata agacaaccgg
      781 actaaaaaag cagcccgggt atatcttcga catcgatgac atacctcatt tgagtgatag
      841 agaaaaggcg cgtttgaagg aagtcacacg cttctttgcc ttccggtcca atgaatatta
      901 tctccgcctg atcgattggg ataatcccaa tgatccgatc cgtcgcataa taattcctga
      961 cgaggatgag ttaaacgact ggggggagat ggacgcctgc aatgagcgtt cggtcacagt
     1021 ccgaaagggc gtacagcaca aatatggctc aaccgtactg ctgctggtgc atgaggtctg
     1081 taactcattc tgccgctact gcttccgcaa acgactcttt attaatggcg gcaacgaggt
     1141 gagctataat gtcgatgagg gcatcgagta cataaaaaaa cataaagagg tcaataatgt
     1201 ccttctgacc ggcggcgacc cgctggtcct gccgacacga aggctggaga agataataaa
     1261 ggccctgcgc gaaatcgagc atgtgcgcat cattcgtctc ggaaccaaga tgaccgcctt
     1321 caatccgtac cgctttatca atgacccgga gctactggaa gtgctttcgc gctattccga
     1381 gccggacagg aagatatacg tagtctgtca tttcgatcat ccccgggaga ttactcctca
     1441 ggcccggcag gcggtacatc tcctgcagaa ggcgggatgt atcctcacaa accagaatcc
     1501 gattatcagg ggcatctcgg ataagcccag tgtcatggcg gagctgtgga atgagctctc
     1561 ctatatgggc atgccccagt attatatctt ccagtgccgt cccaccgccg gcaacgagcc
     1621 ttacgcggta ccgattaccg aggcctattt caagatcgag gaggccaagc ggagatgttc
     1681 cgggctggcc aagcgcttga aatatgtcat gtcgcatgaa acgggcaaaa tcgaagttgt
     1741 gggtgtggac gataatttca tttatatgag ataccatcgc gccaaactcg aagatgatga
     1801 gcag
//
LOCUS       DSEB01000223            1939 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1010811, whole genome shotgun sequence.
ACCESSION   DSEB01000223 DSEB01000000
VERSION     DSEB01000223.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1010811"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..417)
                     /locus_tag="ENO07_03970"
     CDS             complement(<1..417)
                     /locus_tag="ENO07_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HDS01155.1"
                     /translation="MVVPKKFDWSQFTLNIAIKAAPSKVYKAWTDSKEICKWFAYDAE
                     VEPKKGGKFSLTDIYGNSFDMQMLGARKDSRIHFTFGMNDEQVEVKLKKSGRYTVCEL
                     HQFNMKTSPNHKWDTHMEQRAGWTFFLTNLKAWLEHG"
     gene            complement(486..1358)
                     /locus_tag="ENO07_03975"
     CDS             complement(486..1358)
                     /locus_tag="ENO07_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDS01156.1"
                     /translation="MGELEFYIVDVFAEEKYAGNQLAVFRNAGHLSSEQMQKIANETH
                     FSETTFILSDQENNGGYDVRIFTPVEELPFAGHPTLGTAFIIKNEILKQPAEKIILNL
                     GVGQIPVTLAGKLLWMKQIEPEFGQTAEAKKVAGAIGIDFEYIDQRFPIEEVSTGMFC
                     LIVPLKNLNAVRQAAVDREKFRKLIETLLARSVLIFCPEAYDENNDLNVRFFAEELGV
                     AEDPATGSANGCLAAYLVKNRYFDEDRIDIKVEQGYEISRPSLLYLRANEEEGRINVH
                     VGGKVEMIAKGRFI"
     gene            complement(1373..1924)
                     /locus_tag="ENO07_03980"
     CDS             complement(1373..1924)
                     /locus_tag="ENO07_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01157.1"
                     /translation="MTDSSISVGFIPDYSYEGVGVKVESVTEGETLANSIGLQSGDII
                     IGGNTMKIEDMGDLEEFKSQLHRGGGAVLAIDRNSEKFTVYGILPPPERKPLFVYKKY
                     SAMARIKHRGNGIAIEGSRVGAFTIKVDPELLDLDMRIVVRLNLKKYQDDYIEPNLRY
                     ILENFLENRDRKLLYVAEINVDV"
BASE COUNT          466 a          519 c          396 g          558 t
ORIGIN      
        1 cccatgctcg agccatgctt tcagattggt caggaagaat gtccatccgg cgcgctgctc
       61 catgtgggta tcccatttat gattggggct ggtcttcatg ttgaattgat gcagctcgca
      121 gacagtgtat ctgccgctct ttttcaattt tacctccacc tgttcatcat tcatgccgaa
      181 agtaaaatga atccggctgt ccttgcgcgc ccccagcatt tgcatgtcga aagagttgcc
      241 gtaaatatcg gtgagactga atttcccgcc ctttttgggc tctacctcgg catcatatgc
      301 aaaccattta cagatttctt ttgaatccgt ccaggcctta tatactttcg agggcgcagc
      361 ttttatggct atgttcaatg taaactggga ccagtcaaat tttttgggta caaccattat
      421 ccctccttta ttatcatatc cggctgtatc aagctttctt atgtattctg aaagtcgtta
      481 aatactcaga taaatcgtcc ctttgcgatc atctcaacct ttccaccgac atgcacatta
      541 atccgacctt cctcttcatt ggcacgaaga tacaggagtg agggccggga aatctcataa
      601 ccctgctcga cctttatgtc tattctatcc tcatcgaagt aacggttttt taccagataa
      661 gccgccaggc agccattggc cgatcctgtg gccgggtcct cggccactcc aagttcctcc
      721 gcgaaaaacc tgacatttag atcattattt tcatcgtagg cttccggaca gaagatcaat
      781 accgacctgg ccagtagtgt ctcgatcaac ttcctgaatt tttccctgtc cacagcggcc
      841 tgcctgaccg cattcaggtt cttcaacggc actatcaggc aaaacatgcc ggtcgaaacc
      901 tcctctatgg gaaagcgctg atcgatatac tcaaagtcaa ttccgattgc ccccgccacc
      961 ttcttggctt cggcagtctg gccgaattcc ggctcaatct gcttcatcca taaaagcttt
     1021 ccggccaggg tcaccggaat ctgtcccact cccagattga gtataatttt ctcagccggc
     1081 tgctttagaa tttcattctt aattataaag gctgtgccca gggtgggatg ccccgcaaaa
     1141 ggaagttcct cgacaggtgt gaatatgcgg acatcatatc cgccgttatt ctcttgatca
     1201 gacaagatga atgtggtttc agaaaaatgg gtctcattgg ctattttctg catctgctcg
     1261 cttgagagat gacccgcatt gcggaatacc gccagctggt tgccggcata cttttcctct
     1321 gcaaaaacat cgacaatata aaattccagc tcgcccatag tccaaacctc ctctatacat
     1381 caacatttat ctcggcgaca tagagcaact tacggtcgcg gttctcaaga aaattctcga
     1441 gaatgtatct cagattcggt tcaatatagt cgtcctgata ttttttaagg ttgagccgta
     1501 ccacgatcct catgtcgagg tcgagtaatt cgggatcaac cttgatggtg aaggccccta
     1561 ctcgcgaacc ttcaattgct atcccattgc ccctgtgttt gatccgggcc atagccgaat
     1621 acttcttata tacaaacaag ggttttcttt ccggaggagg aaggatgccg taaactgtaa
     1681 atttctcgct gttacggtca attgccagga ccgctcctcc gcctcgatgc aactgggatt
     1741 tgaattcctc cagatcaccc atatcctcga ttttcattgt atttccaccg atgataatat
     1801 cccctgactg caaaccaatg ctattggcca gagtctcgcc ttcggttaca ctttcgacct
     1861 taactccgac gccttcataa gaatagtcag ggataaatcc gacagaaatc gaactgtctg
     1921 tcatggtggt attaagcac
//
LOCUS       DSEB01000224            2689 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1005241, whole genome shotgun sequence.
ACCESSION   DSEB01000224 DSEB01000000
VERSION     DSEB01000224.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1005241"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..460)
                     /locus_tag="ENO07_03985"
     CDS             complement(<1..460)
                     /locus_tag="ENO07_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDS01158.1"
                     /translation="MEVPLLDLTRQYKGIKSEIDQAVHKVLEHTRFIMGPEVEQFEEQ
                     AARYCGSKYAIGVASGTDALLIALRACGVKAGDEVITSTFSFFASAGVISRLGATPVF
                     VDIDPDTYNLKPEEVKKAVSDKTRVIMPVHIFGQCADMDQILQIARENNIM"
     gene            complement(497..1717)
                     /locus_tag="ENO07_03990"
     CDS             complement(497..1717)
                     /locus_tag="ENO07_03990"
                     /inference="COORDINATES: protein motif:HMM:PF13807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01159.1"
                     /translation="MPEKKNSLLIVLRSLVEHRGLILINVFVVTVAAVIISLLLPKWY
                     KATAVILPPEKSESLSEIAASMGLQSLALSGTGFALPVMASPSDLLASIAESRTVAEG
                     VVEDMDLVNVYGVEKKRSAIGILQKQMDVNVRADGIIDISYEEKDPELAANVANSITR
                     QLDNINRLTKVQKATELKRFIMEELEKNEVNLRKAELALQEFQRTHKAISLDEQTAAS
                     INAAAELYSQLTLDQINLKVMEKSHSPDHPDVINLRYKISEIKKRLKEIQEGSSAPED
                     TSTAFLAIPFSEIPEITLKYMQLVRNLKREEALHEVLNTQLEQAKIMQAKDTPTISVL
                     DWAVPPAHKFKPKRVYIVITAMILSFVFSMVYAVAHDRWRDYKLSNPERYKDISVIFS
                     TLKKDLFGFKKRKS"
     gene            complement(1723..>2689)
                     /locus_tag="ENO07_03995"
     CDS             complement(1723..>2689)
                     /locus_tag="ENO07_03995"
                     /inference="COORDINATES: protein motif:HMM:PF10531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01160.1"
                     /translation="PRRTKVGLVEIYGAVQLPGEYEYVSGETINDLIMLAGGITLDAN
                     LNSASLVRFDEAGDSSITLNIPLEAILEDPGNPGNMLLMPDDRIFIRSIPDYHRKAQV
                     TIEGEVLYPGVYAVKEDTTTLTEVIAWAGGFTEEASLAEARMFRSGYEAIRDTELDRQ
                     IKLSVDQLSEIEREYLLLKSDPQQGRVSIDFVELFSNGARGLDVRLKDKDSIIIPKTS
                     LSVRIMGRVLKPGLLTYKSGADVKYYLNQAGGLTKSADKGKIRIIKGASGAILKPSSR
                     VQIEVGDEILVPEKKDVNWWEVTKDVGLFLANLATLYIVIDQILE"
BASE COUNT          619 a          711 c          620 g          739 t
ORIGIN      
        1 ccataatatt gttttctctg gcgatctgca gtatctgatc catatcagcg cactggccga
       61 aaatatgcac aggcatgatt acgcgggttt tatcgctgac agcttttttg acctcttcgg
      121 gcttgagatt atatgtatcc gggtcaatat cgacaaagac aggtgtggct cccagcctcg
      181 agattactcc cgccgaagca aaaaacgaaa aggtcgaggt aatcacctca tcccctgctt
      241 tgactccgca ggcgcgcagt gcgatcagca gagcgtcggt gcccgaggcc acgccgatgg
      301 catatttact gccgcaatag cgggccgcct gttcctcgaa ttgctctacc tccggcccca
      361 ttatgaatct ggtgtgctcc agaaccttat gaacggcctg atctatctcg gattttattc
      421 ccttatactg gcgggtcaaa tctaagagcg ggacttccat tttcctcctc acaattttgg
      481 gtcatgcgac tggatatcag gatttgcgtt ttttgaatcc aaaaaggtct tttttcaggg
      541 tactgaatat gaccgatata tctttatagc gttcgggatt tgatagctta taatcccgcc
      601 acctgtcatg cgccacggca taaaccatcg agaatacaaa actcaaaatc attgcggtaa
      661 taactatata gacgcgtttg ggtttgaatt tatgcgcggg aggaaccgcc caatccagaa
      721 cagagattgt cggggtatct ttggcctgca tgattttggc ctgctcgagc tgggtgttca
      781 ggacctcatg cagtgcctcc tcacgcttta gattgcggac cagctgcata tatttcaggg
      841 tgatttccgg aatttccgaa aacggtatcg caagaaatgc tgtagatgta tcctccggcg
      901 cagagctgcc ctcctgaatc tccttcagcc tttttttaat ttccgagatt ttgtatcgca
      961 ggttgatcac atccggatgg tcgggggaat gagatttttc cattactttc agattaatct
     1021 ggtccagggt aagctggctg taaagctcgg cggcggcatt tatggaagcg gccgtctgtt
     1081 catcgaggga gatagcctta tgggtgcgct ggaattcctg cagagcaagt tccgccttac
     1141 gcagattaac ctcatttttt tccagctctt ccataataaa tcttttcagt tcagtggctt
     1201 tctgcacctt ggtaaggcgg ttaatattat ccagctgtct ggtgattgaa ttcgccacat
     1261 tcgccgccag ttccggatcc ttttcctcat aagaaatatc tatgataccg tctgcgcgca
     1321 cattcacgtc catctgcttc tgcagtattc cgattgcgct gcgtttcttt tcgaccccgt
     1381 agacattgac cagatccata tcctccacca cgccttcggc cacggtacgg ctctcggcta
     1441 tggaggccag aagatcggaa ggggaagcca tgaccggcag ggcaaaaccg gtgccgctta
     1501 acgccaggct ctggaggccc atcgaggccg caatttcgct taaggactcg ctcttttccg
     1561 gagggagtat tactgccgtg gccttgtacc atttaggaag caacaggctt attataaccg
     1621 ccgcgacggt tacgacaaaa acatttatca gtatcagccc gcggtgctct accagcgatc
     1681 ttaagacaat gagaagactg ttcttttttt ccggcataat gattattcaa gaatttgatc
     1741 aattactata taaagcgtgg cgagatttgc aaggaacagc cccacatcct tggttacctc
     1801 ccaccaattg acatcctttt tctccggtac cagaatctca tccccaactt cgatctgaac
     1861 cctggaggag ggtttgagta ttgcccctga ggcacctttg atgattctga tttttccctt
     1921 gtcagcgctt tttgtcaatc ccccggcctg gttcaggtaa tattttacat ccgccccgga
     1981 cttgtaagtc aacagccccg gcttgaggac acgtcccata atacggacgc ttaaagatgt
     2041 tttgggaatt attatactgt ctttatcttt cagcctgaca tccagcccgc gcgcgccatt
     2101 actgaataac tcaacgaaat cgatggacac tcttccctgc tggggatcgc tcttgagcag
     2161 aagatattca cgttcaatct ctgatagctg atctaccgac agctttatct ggcgatcgag
     2221 ttcggtatcg cggatggcct cgtatccgga acgaaacatc ctggcctcag ccagtgaagc
     2281 ctcctcagtg aatcctcccg cccaggctat gacctcggta agggtggtgg tatcctcttt
     2341 gacagcatac acccccgggt aaagcacctc accttcgatt gttacctgag ccttgcgatg
     2401 gtagtccggg atcgagcgga tgaaaatacg atcgtcaggc attaacaaca tattgccggg
     2461 attcccgggg tcctccagta tcgcctccag aggaatattt aatgttatac tcgagtctcc
     2521 ggcctcgtca aatcgtacca ggctggcaga attgagattc gcatcaagcg ttattccgcc
     2581 cgcaagcata atcagatcat tgattgtctc tccgctgaca tactcatact caccgggtaa
     2641 ctgcaccgcg ccgtagatct caaccaatcc aaccttggtc cttctggga
//
LOCUS       DSEB01000225            3701 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002563, whole genome shotgun sequence.
ACCESSION   DSEB01000225 DSEB01000000
VERSION     DSEB01000225.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002563"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..738)
                     /locus_tag="ENO07_04000"
     CDS             complement(<1..738)
                     /locus_tag="ENO07_04000"
                     /inference="COORDINATES: protein motif:HMM:PF13476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01161.1"
                     /translation="MHFYPVKTLFEVLHMQITKLELNRYKNLRSFRETFDRGINLIKG
                     PNEAGKSSIVEAITDLFFTDPGSSRKELKEKIGWDSERSFDLTMEFSIEGQMYRLHKD
                     FEKGETSLLKQSSGEEIVDKKNILNIIESGLGMANRDIYLATTTIRQDEIGTVSKSAD
                     AIKDKLEGMITAGQEEVLASDAINKLESSIRDINKQGSKHLGVIQKLEKNKDELIYEL
                     DKAKREIDLVGKNRAKLKETQGLLEGIR"
     gene            complement(707..1828)
                     /locus_tag="ENO07_04005"
     CDS             complement(707..1828)
                     /locus_tag="ENO07_04005"
                     /inference="COORDINATES: protein motif:HMM:PF00149.26"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HDS01162.1"
                     /translation="MAKILHTGDIHLGAKLAGLGKSGDKVRAALKDAFSKMIDICLES
                     KVDAFVVAGDLFDSNHISKPLLDFAMREMGRLGDIPAIVIPGTHDCLEDNCLYSTLHA
                     NECPENLYLFNNPEKTKFAFPDRGLTFYANSNEGTRSKQSPLAGLTRDSSPGYHIAIA
                     HGSLAIPGKSAADDFPMEVEEIENSGFDYIALGHWHSHMRAPTQKVPASYCGSPEPMS
                     FKQTDAGWAAMVELAEGKVLVEKIQVGRLEWKTIELPCTSYKYTLELEREISKYVGEN
                     KLLKVILTGIFPADGFIDFDRLYEHMMDSFLYLDLDNRSQSIPENLQELNLPETTILG
                     QYIKMLSADIKEEQDPEQQELLRNGLKLGYALLSGKDAI"
     gene            complement(2276..2497)
                     /locus_tag="ENO07_04010"
     CDS             complement(2276..2497)
                     /locus_tag="ENO07_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01163.1"
                     /translation="MRILTWIIHNPLRFASLALMFLGAVAVVVGAVTLASSASAAVGS
                     AWLFGGLLIYLSGSTINRYLRGDFYYQRL"
     gene            complement(2940..3689)
                     /locus_tag="ENO07_04015"
     CDS             complement(2940..3689)
                     /locus_tag="ENO07_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01164.1"
                     /translation="MSRRKIQISILTLIILILAFSNLSAQWEIDLEFGPVFNGYNDVQ
                     IPNETGTRFSLTDDLSVDPDFFFRTRVSFIIDGMHVISGFAAPLRLDAEGSVGKEILF
                     EGTTFPANTELRGFYRFDSYRLTYRYNFIREPDLTIGLGFTAKVRDAEIRIESEDQIA
                     SKTNTGFVPLINFRIDWLFAERFGLLFAGDALAAPQGRAEDVILALKYHIDENLALKS
                     GYRILEGGADVDDAYTFALLHYISFGLILSF"
BASE COUNT          855 a          973 c          877 g          996 t
ORIGIN      
        1 cctgatcccc tccaggagcc cctgggtctc tttcagcttg gcccggttct tgccgaccag
       61 gtcgatctcc cgcttggcct tgtccagctc gtatattaat tcatctttgt tcttttccag
      121 cttctggata acccccagat gtttgctgcc ctgcttgttt atatcccgga tactgctctc
      181 gagcttattt atcgcatccg aggccaggac ctcctcctga ccggctgtta tcatcccctc
      241 cagtttgtct ttgatagcgt cggctgattt ggagactgtg ccgatttcgt cctgccttat
      301 ggttgtggtg gccaggtaaa tatcccggtt ggccatgccc agtccggatt cgataatatt
      361 caggatattc ttcttgtcta caatttcctc accgctgctt tgcttcagaa gcgaggtttc
      421 acccttctca aaatccttgt ggagacgata catctggccc tcaatggaaa attccatggt
      481 caggtcgaat gacctctcag aatcccatcc gatcttctct ttcagttcct ttctggagga
      541 gccgggatcg gtaaaaaaga gatcggttat ggcttcgaca atggatgatt tgccggcctc
      601 attggggcct ttgatcaagt tgattcccct gtcgaatgtc tccctgaatg atctcaggtt
      661 tttgtacctg tttagttcga gctttgtgat ctgcatatgc aatacctcaa atagcgtctt
      721 taccggatag aagtgcatag cccagcttca agccgttgcg cagcaactcc tgttgttcgg
      781 gatcctgttc ttcttttata tcggccgaaa gcatctttat gtactggccg agaatcgtgg
      841 tctcgggcag attaagttcc tgcagattct caggaatcga ctgggagcgg ttatccagat
      901 ccagatacag gaaggaatcc atcatgtgct cataaaggcg gtcgaaatcg atgaaaccgt
      961 cggcaggaaa gatgcctgtc aggatcacct tcagaagctt gttttctccc acatatttgg
     1021 aaatctcacg ttccagttcc agggtatatt tatagctcgt gcacggcagt tcgatcgtct
     1081 tccattccag cctgcccacc tgaatctttt caaccagcac cttgccctcg gccagctcaa
     1141 ccatcgcggc ccatccggca tcggtctgct tgaacgacat cggttcggga gagccgcaat
     1201 atgaggccgg gactttctgc gtcggggcac gcatatggga atgccagtga cccagcgcga
     1261 tataatcgaa gccggagttc tcgatctcct ccacttccat cggaaaatca tcggcggccg
     1321 acttgcccgg tatcgccagt gagccatggg caatggcgat atggtaaccg ggagaggaat
     1381 cccgggtcaa tcccgccagc ggtgactgct ttgaacgtgt gccctcgttt gaattggcat
     1441 agaaagtcag gccccgatca gggaaagcaa atttcgtctt ctcgggattg ttgaaaagat
     1501 aaagattttc cgggcattca tttgcatgca gggtgctgta caggcagttg tcttccagac
     1561 agtcatgggt tccggggata acgattgcgg gaatatctcc cagccgtccc atctcgcgca
     1621 ttgcgaaatc caggagcggt ttggagatgt ggttggaatc gaaaaggtct cctgccacga
     1681 cgaaagcgtc aactttcgat tcaagacaga tatctatcat tttgctgaaa gcatctttaa
     1741 gcgcggcacg gaccttgtct cccgatttac ccagaccggc caattttgcg cccagatgaa
     1801 tatcgccggt atgaagtatt ttagccataa tatttcctgt tttgttagtt aaagacctct
     1861 ttaaacttat cggtctatcc cgccggtatc tgaatccccg cagtagaatt tttgaacaga
     1921 tgggctattt ttttgagagg tatggattta aggaaatcta aaagatcgtg cgcggcccgt
     1981 cagtcggccg ggcaggagta cttcctcgtg ctccgccccg gcggcctgcc aaattcatct
     2041 tctcatctgc cgccgggaac cctttgccgg cggttatctg tcgggttggg gaacccgaca
     2101 gcacaataaa acgccgttcc gtcagttcct cagccaaatg cggttaaccg acggcaaaca
     2161 aaaaagcgat gaatcattta attttgcttg gattcatcgc ttttgatagc cgcaacatgg
     2221 ttcatctatc aaagctctct ccttcccgag tgaggattct tgatcttttc aaacattaca
     2281 atctttgata atagaaatca cctcgcagat atctgttgat ggtggatccg ctcaggtaga
     2341 tcaggagtcc cccgaacagc catgctgatc caactgcagc cgaggcgctc gacgccagag
     2401 ttaccgctcc caccaccacg gcaaccgccc ccagaaacat caatgccagg cttgcaaacc
     2461 tgagcggatt atggattatc catgtcaaaa tccgcatttt tcatcacctc gattcctgtt
     2521 ccggtctgtt aacggtttga ttcttacctt ttcaatgccc cgtcttttga tttacaggtc
     2581 atactacgat ttccggtcga ttttgttgcg attttccgag ggggtggtga tgatttcgga
     2641 gatctgcccg gtttgttttg gggatgcccg ggacaattca tgcgcggcgt acatccccat
     2701 acgccatggc gatgtgactc tcgcttctgc cagggtgccc tgcccaaatt tatttggcgg
     2761 ggctcatgct gcagtaacac gcctaaagcc agttagggcg tgccatcccg acgatctgca
     2821 tttcaatcgc aatagaagac aggcagaatc gctccgcccg tcaaaagccg gatcttcgac
     2881 tttgctgtgg attctaccct gcgctgagcc cgcatttcat gtgcgataca attgtaagtt
     2941 caaaagctga gaatcagacc aaatgaaata tagtgcagca gcgcgaaagt gtaggcatcg
     3001 tcgacatccg cgccgccctc cagaatccgg taacccgatt tgagcgcaag gttctcatcg
     3061 atatgatact tcagcgccag aatgacatct tcagcacgtc cctgaggcgc agcgagcgca
     3121 tcaccggcaa ataatagccc gaatctttcg gcgaaaagcc agtcgattct gaaattgatc
     3181 aatggaacaa atccggtgtt agtcttggag gcaatctgat cttccgattc aattctgatc
     3241 tcggcatcac gcactttggc ggtgaaaccc aaaccaattg tcaggtcggg ttctctgatg
     3301 aaattgtatc ggtatgtcag acgatatgaa tcgaagcggt aaaacccgcg cagctcggta
     3361 ttggctggaa atgtcgtacc ctcgaacaat atttccttgc cgacacttcc ttcggcatcc
     3421 agccgcaacg gcgcggcgaa acccgatatc acatgcatgc cgtcaattat aaaggagact
     3481 cttgtcctga aaaagaaatc gggatcgacc gaaagatcat cagtcaatga aaaacgtgtc
     3541 ccggtttcat tgggaatctg cacatcgttg tagccgttaa aaaccggacc gaactcgaga
     3601 tcaatctccc attgtgcgga aagatttgag aacgccaata tcagaattat cagcgttagt
     3661 atggagattt gtatttttct tctgctcatt tcaatattcc c
//
LOCUS       DSEB01000226            2616 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1005577, whole genome shotgun sequence.
ACCESSION   DSEB01000226 DSEB01000000
VERSION     DSEB01000226.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1005577"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(186..1310)
                     /locus_tag="ENO07_04020"
     CDS             complement(186..1310)
                     /locus_tag="ENO07_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF373 family protein"
                     /protein_id="HDS01165.1"
                     /translation="MQTGRTLVLSVDRDDDIGFKADLVSPIIGRDSCLAAATSLGLAD
                     PEDSDLNAIFQAVQIYDELKEKGEDVEIAIVTGNHMHMIEGDRKIASSLEKVVMETKT
                     SSCIVVTDGAEDEFVIPIVQSKIAVSSIRRVVVNQIPNLEGTYYIIKKLLNDPKVARL
                     FLAPLGLAMMLWAVAYLMNRPQIATIVVVGGIGIYLLYRGLGIDEVFEGFVNALRISL
                     RRAKFSFVTYIAGVLLVIIGVVMGLMNVLVFYAEVGILIYLLIFVFGAVLWLALAGVV
                     SSVGIIIDSYFYDRPNLAKVVAFPFFIGAIGLITYGASIYAITASNVPEFPYTADTAL
                     QYILIGTVGGLICAFAGIGIQYLVQKYLASRRKAELPDAA"
     gene            complement(1374..2234)
                     /locus_tag="ENO07_04025"
     CDS             complement(1374..2234)
                     /locus_tag="ENO07_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HDS01166.1"
                     /translation="MLNELIEKRKKILAESEQHKNRRNELNALASSHARERNTLNGQT
                     REYVEEAQKNKELRDHYNNEVQSLKDQRNEINEQANILFEEIEAFKKDHGGIKNRGIK
                     ELQKQIEHLEFRQQTEVISTDKERELIDKIKQMKESMKEQEAELEQNKEIKAKINSAR
                     DFRKEASELHAKVTEMAELAQKHHDMMVEFYRKADKSREEADNAHKNFVEAQESADAE
                     HKYFIACQKELRDYDKVISGLRKKTKKTKVTKEQKAVRKEAEHLFKMFRAGEKLTTDD
                     ILLLQRSKLI"
BASE COUNT          582 a          661 c          669 g          704 t
ORIGIN      
        1 ggtgtttgaa ttatcagccc cggttctgcc tgccacacac cagtgcgtgg cggtctattc
       61 ctggtatcca acgcggggcg gaaaaaacac ctgattctca tcgagccaca tgcatacaaa
      121 atgaaaatgg agtatgagcg ttcgcgatta tttctcttca caggtccggt gacgtttccg
      181 ggttttcagg ccgcatccgg gagttcggcc tttcggcggg atgcaaggta tttctggacc
      241 aggtactgga tgccgatacc cgcgaaggca cagatgagcc cgccgaccgt gccaatgagg
      301 atgtattgga gggcggtgtc ggcagtatag ggaaattccg gaacattgct cgcagtaatt
      361 gcataaatgc ttgctccgta cgtgataagc ccgattgcac cgataaagaa cgggaaggcc
      421 acgaccttgg ccaggttagg gcggtcatag aagtaactgt cgatgataat cccgacagac
      481 gagaccactc cggcaagggc gagccagagc actgctccaa acacgaagat gagaaggtat
      541 atcagtatgc ccacttcggc gtagaatacc aaaacgttca tgagacccat cacaaccccg
      601 atgatcacca ggaggactcc cgctatgtag gtgacaaagg agaactttgc ccgtcgaagc
      661 gatatccgca atgcattgac aaatccttcg aagacctcgt caattccgag tccgcggtac
      721 aggaggtaga tcccgattcc tccgacaacc acgatcgtcg cgatctgggg gcggttcatg
      781 aggtaggcga ctgcccagag catcatggca aggccgagcg gtgcgagaaa caggcgcgca
      841 acctttggat cattcaggag tttcttgatg atgtaatagg tcccttccag gttcgggatc
      901 tggtttacca ccaccctcct gatacttgat acggcgatct ttgactggac gatggggatg
      961 acaaattcgt cttctgcccc gtctgtaacg acgatacagc tggacgtttt ggtctccatg
     1021 acgacctttt cgagagagga ggcaatcttc ctgtcacctt cgatcatgtg catatgattt
     1081 ccggtcacga tggcgatctc tacgtcctcg cctttttcct ttaattcgtc atagatctga
     1141 acggcctgga agatcgcgtt caggtccgag tcttccggat cggcaaggcc aaggctggtg
     1201 gcagcggcga gacatgaatc ccttccgatg atgggactca ctaagtctgc cttgaaccct
     1261 atatcgtcgt cacgatcgac actcaggaca agcgtccgtc ccgtctgcat cacaatagaa
     1321 atcgtaatca atctattaaa tcgttacttt cgctggggag taaaaagggt atattatatg
     1381 agttttgagc gctggagaag caggatgtca tcagtggtga gcttctctcc tgcccggaac
     1441 atcttgaaga gatgttcggc ttccttgcgc acggccttct gctccttcgt aaccttggtc
     1501 ttcttggtct tcttgcgcag gccgctaata accttgtcat aatccctcag ttccttctgg
     1561 caggcaatga aatacttgtg ctcggcgtcg gcagattcct gggcttcgac aaaatttttg
     1621 tgagcgttgt cggcttcttc ccgtgatttg tcagctttcc ggtagaattc aaccatcatg
     1681 tcatggtgct tctgggccag ttcagccatc tcggtgacct tggcgtggag ttcggaggcc
     1741 tctttgcgga aatccctggc gctgtttatc tttgccttga tctccttgtt ctgttcaagt
     1801 tcggcctcct gctccttcat cgactccttc atctgcttga tcttgtcgat caattcccgc
     1861 tctttgtcgg tgctgatgac ctcggtctgc tgacggaatt ccaggtgctc gatctgtttc
     1921 tggagctcct tgatgccccg gttcttgatt ccgccatggt cttttttgaa tgcctcgatc
     1981 tcttcgaaga gaatattggc ctgctcattg atctcgtttc gctgatcctt gagactctgg
     2041 acctcgttat tgtaatggtc gcggagttcc ttgttcttct gggcctcctc gacgtactca
     2101 cgggtctggc cgttcaaggt gttcctctcg cgtgcgtggg aactggcgag cgcgttcaac
     2161 tcgtttctgc ggtttttatg ctgttcagac tcggcaagaa tcttttttcg tttctcaatt
     2221 aattcgttca acatgcatta ccatcctcga cagggtcggg tcggataccc ggtagaaagg
     2281 agttggagaa taaatgaaaa acagcatgaa ttggactaaa attaagcgtg gtaatactac
     2341 ccaaaacagc caaaatcagt ccatgcaatg aagagaattg ccggtcacca gctgatatgc
     2401 cggatttgtg ggtgttttgg cccttattca tctgcctgtt cattatgttc aactcccgat
     2461 accagaaggg tgcggaaaat cttggtgtgt ctggcgccct tcaaaccagc actcctttcg
     2521 tgagggatat tccaaagatt ttctattatg tagaagacac ggatcctatt aaggatttcg
     2581 ggagatcgga aaaaaagaaa atcaggtggc gaaacc
//
LOCUS       DSEB01000227            2688 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1005249, whole genome shotgun sequence.
ACCESSION   DSEB01000227 DSEB01000000
VERSION     DSEB01000227.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1005249"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..209
                     /locus_tag="ENO07_04030"
     CDS             <1..209
                     /locus_tag="ENO07_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDS01167.1"
                     /translation="LDVIAAVLLVVGGLNWGLVGFFGFDLVASIFGPMSFLSRIVYIM
                     VGIGALYQAAQWKAIQRRWQPVTA"
     gene            complement(475..846)
                     /locus_tag="ENO07_04035"
     CDS             complement(475..846)
                     /locus_tag="ENO07_04035"
                     /inference="COORDINATES: protein motif:HMM:PF13473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01168.1"
                     /translation="MQYFKPFILLLAIVLFLGCSDDNGDTGTNPPPGNGTAARIDVSM
                     VNTTFSPADITINVGDTVVWTNNDQIIHTTTSTGGVESWDSGNMSSGETFRKVFNNVG
                     TSNYRCEIHPVVMTGTVTVEQ"
     gene            1156..1410
                     /locus_tag="ENO07_04040"
     CDS             1156..1410
                     /locus_tag="ENO07_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HDS01169.1"
                     /translation="MPDEPVGCARPTGKVVGEETPSSEVSEEKTKTGVQNMSMAKVGG
                     KAPDFEAPAYHRGKFINVKLSDYLGKWVLICFYPGDFTFV"
     gene            1420..1869
                     /locus_tag="ENO07_04045"
     CDS             1420..1869
                     /locus_tag="ENO07_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HDS01170.1"
                     /translation="MSAVAGKFDQLKELGVEVLSISTDSQFVHKIWEEEELSKMTKDG
                     IPWPMVADGAGNLGRVYGVYDEANGVNIRGRFLIDPDGVIQAMEVLTPPVGRKFEETI
                     RQIQAFQHVRNTKGAEACPAGWEPGKPTLKPGPDLVGKVWKVWQPEK"
     gene            complement(1984..>2688)
                     /locus_tag="ENO07_04050"
     CDS             complement(1984..>2688)
                     /locus_tag="ENO07_04050"
                     /inference="COORDINATES: protein motif:HMM:PF07478.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDS01171.1"
                     /translation="GGTPGFLHVGLDKRLSGKLLAVRGIKVPQGALITLEEPDIPDDM
                     EYPVFIKPNYEGSSMGITQESVAETPEEAKKVVKKLLNEYSEGVIVEQYIEGRELTVP
                     MLQAFSGYLLEIVEYKFEGKEHGIFDYDTKASGGKDESLNTICPPELTPEERRDVLTL
                     ADRAFNVMKIPDFARADIRLTKDGTPYLIEVNPLPGLRPVSPLITGAEAKGLSYNDVI
                     DLIIKSAKERYGIKVD"
BASE COUNT          666 a          642 c          697 g          683 t
ORIGIN      
        1 cattagatgt aatcgcagca gttctgctgg tagtcggcgg tctcaactgg ggcctggttg
       61 gatttttcgg atttgacctg gtggcctcga tctttggacc gatgagcttc ctgagccgca
      121 tagtctatat tatggtcggt atcggcgcgc tgtatcaggc tgcccagtgg aaagcgatcc
      181 agcgccgctg gcagccggtg actgcctgac gggcactttt tcagcaaaat gatgtttact
      241 aaaatggaaa ggttttaacg aagagggtag ttgagaaaac taagagacga gttgtgcaag
      301 caagagattt ttaatcagaa agcgcagtta tcagctgcgc ttttttttcg acatagcctt
      361 gagcttgccg ttcaagccct gatatgtccc tgccacaacc cggcacgagt aaacacttaa
      421 ccaggtcacc aaaccctcag aaattcttta atctcggggt tacaggtatt aatgctattg
      481 ttcaaccgtg acggtaccgg tcatgactac cggatggatc tcgcagcggt aattactggt
      541 accgacatta ttgaatactt tcctgaaggt ctcgccgctg ctcatattgc cggaatccca
      601 tgattccaca ccgccggtcg aggtagtggt gtggattatc tgatcattgt tggtccagac
      661 cacagtatca ccgacattga tggttatatc agccggcgaa aaggtggtgt tgaccatgct
      721 gacatcgatc cgtgccgctg tgccgttgcc cggcggcgga ttggtgccgg tatcaccgtt
      781 atcatcactg catcccagaa ataagactat tgcgagtaaa agtataaacg gtttgaagta
      841 ctgcatacta tcctgccttt ttattaatta aaaagtggta actttcaacc atctaaaaca
      901 ggataaatat agtctttgtt ccactcaact ggttctcaag atagcggcgg ggggcgatat
      961 tattcggatc aggcgggaaa ccattccgca ctgcagtaaa tttccccggg gttacaggcg
     1021 gatatccgac cgcagagata aacatatccg gcatttatac ttagattagt cttgcgaaac
     1081 cctcaaataa atgataattt aggtttgaag gtgacttgcg attcgacatg atcatgtcag
     1141 agacaggagg tgcttatgcc ggatgaaccg gttggatgtg cgcggccgac aggcaaggtt
     1201 gtcggtgagg agacgccgtc cagtgaagta tcagaggaaa aaaccaagac aggagtacag
     1261 aatatgtcta tggcaaaagt gggcggaaag gcgcctgatt tcgaggcacc agcctatcat
     1321 agaggaaaat tcataaatgt taaattatcg gattacctgg gcaagtgggt gctgatatgc
     1381 ttctacccgg gtgatttcac atttgtctga ccgaccgaat tgtcggcggt cgccggcaaa
     1441 ttcgaccagc tcaaggaatt gggcgtagag gtactttcga tttcaaccga ctctcaattc
     1501 gttcataaaa tctgggaaga ggaagagcta tccaagatga ccaaggacgg catcccctgg
     1561 ccgatggtgg cggatggggc cggtaatctt ggcagagtat atggcgtcta cgatgaggcc
     1621 aatggggtca atattcgcgg acgtttcctg attgacccgg acggcgtgat tcaggctatg
     1681 gaggtcctga ctcctccggt cggccgtaag ttcgaggaga caatccggca aattcaggcc
     1741 ttccagcatg tacgcaatac caagggcgcc gaagcctgcc cggcgggatg ggagcccggc
     1801 aaaccgacat taaagcccgg ccctgatctt gtgggcaagg tctggaaagt ctggcagcct
     1861 gagaaataga aatggatctt aaaagtcgtt ggtcaggatc cccgctttcc tgacaggact
     1921 gtaagcggcg tgcataacag cgcgccgctc gattttttgt aagcttgctt tttctgatga
     1981 agtctaatct acttttatcc cgtagcgttc tttggcagat ttgattatca ggtcaatcac
     2041 atcattataa ctcaggccct tggcttcggc gccggtgatc agcgggctta ccggcctcaa
     2101 gccgggcaat ggattgactt cgatcagata gggggtaccg tctttggtca gtcggatgtc
     2161 cgcacgcgcg aaatctggaa tcttcatgac attgaaagcg cggtcggcca gggtcagaac
     2221 atcgcgcctt tcctcgggag tcagttctgg aggacaaatc gtattcagcg actcatcttt
     2281 tcctccgctg gccttggtat cgtaatcaaa gataccatgc tccttgccct caaacttata
     2341 ttcgacaatc tccagcagat agccggagaa ggcctgtagc atcggaacag tcaactcacg
     2401 tccttcgata tactgttcga caataacgcc ctccgagtat tcattcagca atttcttgac
     2461 cactttctta gcttcttcag gtgtctcggc cacagattcc tgagtgatcc ccatgctgga
     2521 accttcataa ttaggcttga taaaaacagg atactccatg tcatcgggaa tatccggttc
     2581 ctcgagtgta atcaaagcgc cctgaggtac ttttatgcct ctgaccgcaa ggagctttcc
     2641 tgacagacgt ttatccaaac cgacatgcag gaacccgggg gtaccgcc
//
LOCUS       DSEB01000228            3075 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003906, whole genome shotgun sequence.
ACCESSION   DSEB01000228 DSEB01000000
VERSION     DSEB01000228.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003906"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(193..1245)
                     /gene="hypE"
                     /locus_tag="ENO07_04055"
     CDS             complement(193..1245)
                     /gene="hypE"
                     /locus_tag="ENO07_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015171967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HDS01172.1"
                     /translation="MDADPREKECPIPKDKYPHIRMAHGGGGRLMHELIEKLFVAAFY
                     SSSLESEHDSAVIHAPSGRLAYTTDSYVIQPIFFPGGDIGKLAVCGTVNDLAMSGARP
                     LYISVGFILEEGLEIETLWKICLSMRREAANAGAKIITGDTKVVDKGKCDQIYINTAG
                     IGIIEHDLNIRPSSIRPGDAVILNGDIGRHGMAVMAKRERLSFDTEIESDCISLSDVI
                     MKLLEAGIEIHCLRDLTRGGLATACVEIARSSKFRIELEEKSIIISKGVRGACEMLGF
                     DPIYVANEGRFVMFVKPGDAGKALVIINAADHDFSPAIIGKVTKEESGLVTLKTRIGT
                     ERIVDMLSGEQLPRIC"
     gene            complement(1246..2355)
                     /gene="hypD"
                     /locus_tag="ENO07_04060"
     CDS             complement(1246..2355)
                     /gene="hypD"
                     /locus_tag="ENO07_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007735088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HDS01173.1"
                     /translation="MKYIDEYHNPRAVAAFAEKIKSITSRDWNIMEVCGGQTHAIVRH
                     GLDQLLPDRITLLHGPGCPVCVTPLSIIDKAIEIAGRENVIFCSFGDMMRVPGSAGDL
                     FSVKSAGGDLRVVYSPLDALRIAQENPDKEVVFFGIGFETTAPANAMSILQAGLVGLE
                     NFSVLSAMVLIPPAMEVILSSDNNKVDAFLAAGHVCTVSGFEQYEELSAGYSVPIVVT
                     GFEPLDIIQGIYMSLAQLEDGRAMVENQYSRSVKREGNAEARRVMEDVLTIVDRRWRG
                     LGMIESSGLAISDKYRQYDAEEKFGKVDDISDQSGECISAEILKGTRKPFDCPAFGTR
                     CTPENPLGATMVSSEGACSAYYSYRKLDLQKTGLE"
     gene            complement(2352..2603)
                     /locus_tag="ENO07_04065"
     CDS             complement(2352..2603)
                     /locus_tag="ENO07_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HDS01174.1"
                     /translation="MCLAIPGKIIGIERDDSLMHTGRVNFGGVIKQINLSLVPDANIG
                     DYVIVHAGFAISMVDEDEAQRVFEYLDEIDGMMEEADRQ"
BASE COUNT          779 a          843 c          622 g          831 t
ORIGIN      
        1 ccataatttc tagtcctctt tttctcacta acttcatcat atcgcccatt aatagtcaaa
       61 ttgcatgcca aagagcgtaa cctattaata gacaataaga agagtttaat gcgggaaata
      121 aacgggatga aaattgtgga atattctaca tttttgcaga ctattctcca gaagattgga
      181 ggtatctgga aatcagcaga ttctgggaag ctgttctccg cttaacatat caactattct
      241 ttcggtgcct attctggttt ttaatgtaac cagacccgat tcttcttttg taaccttccc
      301 gatgattgcg ggtgaaaaat cgtgatcagc cgcattgata atcacaagtg ctttcccagc
      361 atcgccgggc ttaacaaaca tgacaaagcg cccctcattg gcaacataaa tcggatcaaa
      421 cccgagcatt tcgcaggccc cgcgcacacc cttgctgatg atgatggact tctcttccaa
      481 ttctatccgg aatttcgaac tgcgggcaat ttcgacacaa gctgtggcca gacctccgcg
      541 ggtaagatcg cgcaggcagt gaatctcgat gccggcttcc agcagtttca tgataacatc
      601 tgaaagggat atacaatcgc tttctatttc agtatcgaac gacagcctct cacgttttgc
      661 catcaccgcc attccatgcc ttccaatatc cccgttaagg attacagcat cccccggcct
      721 tatacttgag ggcctgatat tcaaatcgtg ttcaataatt ccgattcctg ccgtattaat
      781 ataaatctgg tcgcatttac ctttatcgac tactttcgta tcgccggtaa taattttagc
      841 tccggcattt gcagcctccc ttcgcatcga caggcagatt ttccagagtg tctcgatttc
      901 cagaccttct tccagaataa atccgacgct tatatacagg gggcgcgccc cgctcatggc
      961 cagatcattg accgtaccgc aaacagccag cttgcctata tcgccgcccg gaaaaaagat
     1021 cggttgtatg acatatgaat cggtggtata agcaagcctt ccagacggtg catgaataac
     1081 cgcgctgtca tgctcgcttt caagactgga agaataaaag gcggctacaa aaagcttttc
     1141 tatcaactca tgcatcaggc gtccaccgcc cccatgagcc atcctgatat gcggatattt
     1201 atcttttgga atggggcact ctttttctct cgggtcagca tccatctatt caagccctgt
     1261 cttttgcaga tccaacttac ggtagctata gtaagcgctg catgcgccct cggaggaaac
     1321 cattgtcgcc ccaagcggat tttcgggagt acatctggtg ccaaatgccg gacagtcgaa
     1381 tggtttccgt gtgcctttca gaatctcagc gctaatacat tccccgcttt gatccgaaat
     1441 atcatcgact tttccgaatt tctcctcggc atcatactgt ctgtacttat cgcttattgc
     1501 aagaccgctg gattctatca tcccaagccc gcgccatctt ctatccacga ttgtcaatac
     1561 atcttccata accctgcgcg cctctgcatt tccctctcgt tttaccgaac gggaatactg
     1621 attttcaacc atagctcttc catcctcaag ctgcgctaaa gacatatata ttccctggat
     1681 gatatccaga ggctcgaatc cggtgaccac aattggaaca ctatatcctg cagacaattc
     1741 ctcgtactgt tcgaagccgc tgacagtgca tacatgaccg gccgcaagaa aagcgtcgac
     1801 tttattatta tctgatgaca atatcacttc catggccgga ggaatcagaa ccatcgcaga
     1861 taaaaccgag aagttctcga gtccaaccag accggcctga aggattgaca tcgcattggc
     1921 aggcgccgtg gtctcaaatc cgatcccgaa aaagactact tccttgtcag gattttcctg
     1981 tgcgattctg agcgcgtcaa gaggagaata gactacacga agatcgcccc cggcagactt
     2041 aacactaaac agatctcctg ctgatcccgg cacgcgcatc atatccccga atgagcagaa
     2101 tatcacattt tcgcggccgg cgatctcgat tgccttgtcg ataatgctga gcggggtgac
     2161 acagaccggg catccggggc cgtgcaaaag cgtaatcctg tcgggcaaga gctgatccag
     2221 cccgtgtctg acaatggcat gggtctgtcc cccgcagacc tccattatgt tccagtctcg
     2281 gcttgttatg ctttttattt tctctgcaaa agcggccaca gcccggggat tatgatattc
     2341 gtcgatatat ttcattgcct gtccgcctct tccatcatgc cgtcaatttc atcgagatat
     2401 tcaaagactc tctgcgcctc atcctcatca accatgctga tagcaaaacc ggcatgcaca
     2461 atcacgtagt caccaatatt agcatccggt acaagtgaca ggtttatttg cttgataacc
     2521 ccgccgaaat tgacacggcc ggtgtgcatc aaagaatcgt ctctttcaat gccgataatc
     2581 tttcccggta tggccagaca catttatctt actccataga ttcttctctg gaaactgcca
     2641 taatctgacc caacgagatc ccgccgtcat taggtggaac caggttgtgc cagtatggca
     2701 gaaagccctc tttcttcagg cggtcaaccg ctttttcggt caaatacttg ttctggaaac
     2761 atccgcccga aagcacgaca cgcttcttcc cataaatctt cgcaatctca acaatcatct
     2821 cagccagtgt attatgaaat cttgcggcaa ttcggtttat ggaaatatct ctaccatagt
     2881 ccttgttgat gtcattgatc atcttttccc aatctataat atgtatatct ccctttctgg
     2941 caagatcaaa tgagtagctc ccgagatcat cagacttctc cgccgcaaat tccagcagca
     3001 tggctgcctc tccctcgaag tcgatttcgt ggcaaagtcc gattattgag gcgaccgcgt
     3061 caaacaggcg tccca
//
LOCUS       DSEB01000229            3091 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003861, whole genome shotgun sequence.
ACCESSION   DSEB01000229 DSEB01000000
VERSION     DSEB01000229.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003861"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="ENO07_04070"
     CDS             complement(<1..1152)
                     /locus_tag="ENO07_04070"
                     /inference="COORDINATES: protein motif:HMM:PF07676.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01175.1"
                     /translation="MYALKSSHLFQTPKWVAVLISSALLVIMLSASVLSRGMTVEDVA
                     NLQYAYEIAISPDGQLIAYTLIVRPNPFVEGDGEANRHLYVTDREGNPRPYITGEVTI
                     KNIAFTPDGQYITYIAERKNDNGDEDKDDALYKIPIAGGESRKIIEFATDIRDYTWSP
                     DGRQVAFLAKDSLADEEEELEEKGFDQEIYEEDWRHVRVWILDTDDPGAKPRRLELPG
                     TASSITWSPDGKTLAMALAPTPLIDDTYMYRRVTTVDAKTGGIIYKFDNPGKLGDIVW
                     SPDSRHLAIISARDINDPKEGEIYILNAEGGQLQPVLTDVDGHIRDIFWIDSQTILYL
                     EHQNVHSIIGTINASGTVQKLLISEPRPVFDGLKLSSNKKDVVLLGESPT"
     gene            complement(1220..>3091)
                     /locus_tag="ENO07_04075"
     CDS             complement(1220..>3091)
                     /locus_tag="ENO07_04075"
                     /inference="COORDINATES: protein motif:HMM:PF03169.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HDS01176.1"
                     /translation="FTIKAILVGAFFGILFGAANAYLGLLVGITVSTSIPVAVMSVGL
                     FRFIQKISGGRASILESNIAQTTGSASSSLASGVIFTIPALYLWGFDPSLWQMAGLAL
                     AGGLLGVLFMIPLRRFLIKEEHGRLPYPEGTACAKVLISSDKKGASAKNVFLGLGIGL
                     VYKFVTGMLFLWKEKVAVNLPFINKAQLGLNATPALLGVGYILGFRIATIMVAGGLIS
                     WLFLIPLFGYYGDQWAGSLFPAGETSISSMDPSTIWNNYIRYIGAGAVAFGGIITIIR
                     SIPTMIRSFKIGMEQIKSRIEKNRRGEEDQTERVDQDLSLKYVLFGVIIIVLAILLIP
                     RLLGHGTTFSMRLIAAPGIAIFAFFFVTVSSRIVGLIGVSSNPTSGMTIVTLLAISMI
                     FVGLGWTDIASKAAVLTVGTVVAVAASIAGDTSQDLKTGFLVGATPSKQQISELIGAA
                     SSAVAVCFAITILAKTYTFGSEQLPAPQALLMKTIIEGVLETGIPWGLVLIGVGFGVL
                     AELVRIPSLPFAVGIYLPVSTMTPIFFGGAIRKYIESRQKHDAAERVRRREAGVLFGS
                     GLIGGQGFASVIIAFYAFYAGAPKGLGFEWPAPWGDLVSFAIFLILGYVLFRRTRFS"
BASE COUNT          756 a          823 c          775 g          737 t
ORIGIN      
        1 agttggcgat tcaccgagaa gcacaacatc ttttttgttg ctggaaagct taaggccgtc
       61 aaaaacaggc cgcggctcgg aaatcagcag cttctgtacc gtacccgagg cgttgattgt
      121 gccaatgatc gagtggacgt tctggtgttc aagatacagg attgtctggc tgtcgatcca
      181 gaagatgtcc ctgatatgac catcaacatc ggtcaggaca ggctgcagct ggccgccttc
      241 tgcatttagt atatagattt cgccctcctt gggatcattg atatccctgg cgctaatgat
      301 tgccagatgc ctgctgtccg gactccatac tatatctccc agcttgccgg gattatcaaa
      361 cttatatatt atcccgccgg ttttcgcatc cactgtcgtc acccgccggt acatataggt
      421 atcatcgata agcggagtgg gcgccagcgc cattgccagg gtttttccat caggactcca
      481 ggtaatgctc gaagctgtgc cgggtagctc cagtctcctg ggtttggccc cgggatcatc
      541 ggtatcaagt atccagactc tcacatgccg ccagtcctcc tcgtagattt cctggtcgaa
      601 tcctttttcc tcaagttctt cttcttcatc cgccagtgaa tctttggcca gaaaagcgac
      661 ctgacgtccg tcgggcgacc aggtgtaatc cctgatatct gttgcgaatt cgattatttt
      721 gcgtgattcg cctcctgcga tcgggatttt gtaaagggcg tcgtccttgt cttcatcacc
      781 attatcattt ttacgctcgg cgatataggt gatgtattgg ccgtcaggag tgaaggcaat
      841 attttttatt gtcacctctc cggtgatata tgggcgagga ttaccttccc gatcagttac
      901 ataaagatgc cggttggctt caccgtcccc ttccacaaac gggttggggc ggacgatcag
      961 ggtgtaggct atgagctgtc catcgggaga tattgcgatt tcataggcat attgcagatt
     1021 cgccacatcc tccactgtca tacctcggga caggaccgaa gctgaaagca tgatgaccaa
     1081 aagagcgctc gaaattaata ccgcaaccca tttcggtgtt tgaaataaat gggatgattt
     1141 taatgcatac attgatttat gacctccatt tgttctgcca acacaaatct attattgaaa
     1201 taaggaacta caacatatct caactgaatc tggtgcgccg gaagagaaca tatcccagga
     1261 tcaggaatat cgcaaatgat accaagtctc cccatggcgc cggccattcg aaaccaagac
     1321 ctttgggcgc gccggcataa aatgcgtaga atgcaatgat caccgaagca aatccctgac
     1381 cgccaatgag tccggagcca aaaagcacac ccgcctcacg gcgtctgacc cgttcggcgg
     1441 catcatgctt ctggcgcgat tcaatatatt ttcggattgc cccgccgaaa aagatcggag
     1501 tcatggtcga gaccggaagg taaattccca cagcaaaggg gagcgagggt atccgcacca
     1561 gttccgcgag cacaccaaaa cccacgccta tcaaaaccag gccccaggga atgccggtct
     1621 ccaaaacccc ttcgattatt gttttcatca ggagggcctg cggcgcagga agctgctccg
     1681 aaccaaatgt gtaagttttg gccaaaatag taattgcaaa acaaactgcc acggcagagc
     1741 tggccgctcc aatcaattcg cttatctgct gcttgctggg tgtggctcct accagaaatc
     1801 ctgttttcag gtcctgtgag gtatcccccg caatcgaagc cgctaccgcc accactgtgc
     1861 ctactgtcag cactgccgcc ttgctggcga tatctgtcca tcccagtccg acgaagatca
     1921 tactgatagc cagaagcgtg acgatcgtca ttccggaggt gggattcgag gacacgccga
     1981 tcagtcctac aattctggat gatacagtaa cgaagaagaa ggcgaagatt gcaatgccgg
     2041 gcgcggctat caggcgcatg ctgaatgttg taccgtgccc caggagcctg gggataagca
     2101 ggatcgcaag cactattata atcacgccga agaggacata ttttaatgag agatcttggt
     2161 cgaccctctc ggtctgatct tcctcaccgc ggcggttctt ttctattctt gattttatct
     2221 gctccatgcc gattttgaaa gaacggatca tggtcggtat ggatcttatg atggtgatga
     2281 ttcctccgaa agcgacagct cccgcgccaa tatagcggat gtagttgttc catatcgttg
     2341 acgggtccat gctgctgatt gaagtctcgc ccgccggaaa aagggaacct gcccattgat
     2401 cgccataata gccgaacagc ggaatcagga acagccatga gatcagcccg ccggccacca
     2461 tgatcgtggc aatcctgaag ccgagaatgt agcccacgcc gagaagcgcc ggggtggcat
     2521 tcagccccag ctgcgccttg ttaataaatg gcaggttgac ggcgactttc tctttccaga
     2581 gaaagagcat accggtgaca aatttgtata ccagccctat tcccaggccg agaaatacgt
     2641 tcttggcgct ggcgcctttc ttgtcgctgg atatcagaac cttggcgcag gcggtgccct
     2701 cgggataggg aagccggcca tgctcttcct tgatgaggaa tctccgtaat ggaatcataa
     2761 aaagtacccc cagcagtcct cccgcaagcg ccagcccggc catttgccac aggctcggat
     2821 cgaatcccca gaggtagagg gctggaattg tgaaaatcac tcccgatgcg agcgaggaac
     2881 tggctgaacc ggtggtctgg gcaatattgc tttcgaggat actggcacgc cccccggata
     2941 tcttttgaat gaaacgaaag agccccaccg acataaccgc taccggtatt gatgttgaaa
     3001 ctgttatccc caccaggagt ccaagatagg cgttagcagc gccgaaaagg atgccaaaaa
     3061 aagcgcctac caggatggct ttgatagtaa a
//
LOCUS       DSEB01000230            2080 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1009228, whole genome shotgun sequence.
ACCESSION   DSEB01000230 DSEB01000000
VERSION     DSEB01000230.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1009228"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            331..1374
                     /locus_tag="ENO07_04080"
     CDS             331..1374
                     /locus_tag="ENO07_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018397422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase"
                     /protein_id="HDS01177.1"
                     /translation="MAYKELPIPENFNEEKVPEVWRIEYQELSKRAERWAKEHSLKAA
                     AEDKKTICLLLVDCQNTFCTPGFELFVAGRSGNAAVEDSKRICKFIYRNLGIISNLIV
                     SMDTHKIMQIFHPFFLVDSNGNHPDPMTIVSSEDVENGRWMVNPDVAKNIPDRDYDSL
                     QKFLYHYCRELEESGKLQLMIWPYHSMLGGIGHALVSAIEEALFFYSIARKKQPKIEI
                     KGENQLTENYSVFRPEVTEDEKHQQIASENKAFFDNLLSHDRIIIAGQAKSHCVSWTV
                     QDLLTEIERRDRGLARKIYLMEDCSSAVVIPDSVDFTEKADETFQRFSDAGMNLVKSS
                     DPVASWPGMDFLN"
     gene            1429..>2080
                     /locus_tag="ENO07_04085"
     CDS             1429..>2080
                     /locus_tag="ENO07_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDS01178.1"
                     /translation="MKPVNGYVKPLLTDLYELTMAYAYFNADRHEDFAVFDLFFRKNP
                     FGGEFTIFAGLSEALKFLQDYHFEDDHIDYICEQLPQCSDKFRSWLKGLDCSRVKMNS
                     LREGDLAFPRIPMMRVEGPLGICQLLESTLLNLVAYPSLIATNAMRMRLAAGKDKKLI
                     EFGMRRAQGPDGAVSASRYSYLGGFDGTSNMMAGYLFGMPVSGTQAHSYISSYTSID"
BASE COUNT          572 a          453 c          514 g          541 t
ORIGIN      
        1 cgtatccgat gcggtctggg tcatcaacta cgcctttgtc ggcggtgatc ctcctaacaa
       61 ttgttcacct ggatcatggc ccggcggaga ctgctgtccg ttctaaataa aaagtcatca
      121 gagtgagaag agaccattct ttctcattcg aatgatacaa ttaaagccgt ccggctaaaa
      181 ccgggcggct ttttagttga aaatattgtt ttataatttg caatatacct gctatagtcc
      241 atattgtgaa tagcgaacaa ttgttatttc gttctgttgc agaggatata aactggacaa
      301 atgacaactc agaaaaaggg ataaaggcaa atggcttata aagaattgcc gataccggag
      361 aatttcaatg aagaaaaagt ccccgaggtc tggcgcatcg aatatcagga actttcaaaa
      421 agggccgaaa gatgggcaaa agagcatagc ctaaaagccg ctgctgagga taaaaaaaca
      481 atatgccttc ttttggtgga ctgccagaat acattttgca ctcctggatt tgagctcttt
      541 gtagcgggcc gatccggtaa tgccgcggtt gaagacagca aacgcatctg caaattcata
      601 taccgcaatc tgggcattat ttccaatctg atcgtttcta tggatacaca caagattatg
      661 cagatatttc atcccttctt cctggtagat tcgaacggca atcatcccga tccgatgacc
      721 attgtatcct cagaggatgt tgaaaatggc aggtggatgg ttaatccgga tgttgccaaa
      781 aatatccctg acagagatta cgacagcctt cagaaattcc tttatcatta ttgcagggag
      841 ctcgaggaga gcggtaaact tcaattgatg atctggcctt atcactctat gctgggtggt
      901 atcgggcatg cgttagtatc tgcgatcgag gaggcgctct ttttttacag cattgctcgc
      961 aagaagcagc caaaaatcga aataaaggga gaaaaccagt tgaccgagaa ctactctgtt
     1021 tttcgtcccg aggttaccga agatgaaaag catcagcaga ttgcctctga gaacaaagct
     1081 ttttttgaca atcttctcag ccacgatcga ataataatag ccggccaggc caaaagccat
     1141 tgcgtatcct ggaccgtgca ggatctttta accgaaattg aaaggcgtga tcgagggctt
     1201 gcccgaaaaa tctatctaat ggaggattgt tcatcagctg tggttatacc ggattcggtg
     1261 gactttaccg aaaaagccga tgagacattt cagaggtttt ccgacgcggg tatgaatctg
     1321 gtgaagtcat ccgatccagt ggcttcctgg ccggggatgg acttcctgaa ttaggatttg
     1381 aatcgccaaa ccagacttgc atataaaatg ctgtcaaagg aggataatgt gaagccggta
     1441 aatggctacg tcaagccgct tttaacggat ctatatgagc tcacaatggc ttatgcctat
     1501 tttaatgcgg acaggcatga agatttcgca gtattcgatc tattttttcg caagaatccg
     1561 tttggcggag aattcactat ttttgcaggc ctctccgagg cgctgaaatt cctccaggat
     1621 tatcattttg aggatgatca tatcgattac atctgcgagc agttaccgca atgcagcgac
     1681 aaattccgga gctggctgaa aggactggat tgctccaggg tgaaaatgaa ttccctgagg
     1741 gagggggacc tggcatttcc acggatacca atgatgcggg tggaggggcc tctcggaatc
     1801 tgtcagcttc tggaatcgac actgctgaac ctggtggctt atccttcttt aattgcaacc
     1861 aatgccatgc gcatgcggct cgctgccggt aaggacaaaa agctgatcga attcggcatg
     1921 cgccgtgcgc aggggcctga cggagcggtt tcggcctcac gctacagtta cctgggaggc
     1981 tttgacggaa caagtaatat gatggcgggt tatctcttcg gcatgccggt cagcgggacc
     2041 caggcgcact catatatctc gtcatatacc agcatagata
//
LOCUS       DSEB01000231            3089 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003867, whole genome shotgun sequence.
ACCESSION   DSEB01000231 DSEB01000000
VERSION     DSEB01000231.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003867"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1977)
                     /locus_tag="ENO07_04090"
     CDS             complement(<1..1977)
                     /locus_tag="ENO07_04090"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00593.22,HMM:PF13620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDS01179.1"
                     /translation="MTDKTAKLLSPGFKPAFLFLTLLILIPIAVFARSGLSLWGCITD
                     LESGLPIENATVQIVPEGRIVRTDKDGRYQIYNLKYESVTIEVHAEAYESVRSEKVSL
                     EAALSTRQDFALEKRVYYDPDQIVVAASDGEAAGKVVIADSSPEFRNARTVAEILEFM
                     PQIIVSRAGGTNQTASLSIAGAPAKHTGIFIDGMPVNSRLTGEFDLNSIPKQAVKQIE
                     IYTDGAGAEMGTGSLAGAVNIVTRKAEFEGEIGFEKSLGSFNFDETNMTIQNIFHQKL
                     SGLFVFSRNTASNDFKYNDPKAGESMRQNNYRDLDSRFASLNYKINRHDNVYLTFSDV
                     GSRAGLPGAMYSLTPTAGKSESFRTWNLGTELSPLKNVKLSASTQYYLSHQHFTDYDS
                     FIPYDSKYRDSRLSFNLNPRYIFDPDHFLSARLSLNFDRFKQKYMLGHEQEPIEVGED
                     RMESNLTYNGRLGINDARLFFDFLNVKAAFSQTASELFRPLISPLARVDLEKGRSDKL
                     MLHFSYSNSYRAPTYASLFWSEDAFSVGNPDLKPEKSEDFRTGLRFLFRCAGRWNLGL
                     EYEHSYIKDLIYWERRFDGKYSPRNLSAARVAAVRWNAEWKSSNNLVNIAFSYSLSDP
                     RDRSWEPNQHDNLLIFRPRKMSDLKLSLSPGTIFI"
     regulatory      complement(2007..2204)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(2245..>3089)
                     /gene="rlmN"
                     /locus_tag="ENO07_04095"
     CDS             complement(2245..>3089)
                     /gene="rlmN"
                     /locus_tag="ENO07_04095"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDS01180.1"
                     /translation="PKVLEVKVSRDQTVKYLFELEDGNLIEAVKIPDHPRLTLCMSSQ
                     VGCPLGCKFCKTAQIGFKRNLEVGEILSQLAHVQKTLEPEERISNLVFMGMGEPFLNY
                     DNVKTAVEIIMSVLAFGIGHSKITISTAGHVPGIYRMTDDGLKVRLAVSLNSADPETR
                     RRLMPITRKYSLDELKKSLTYHTSQSRRRVTFEYLLISGITDTINAAKKLVKFVDGIP
                     CKINLINYNPSPGLPSEFRPSTENDINRFRDYLYPRTPAVTIRASKGIDIKAACGQLA
                     AEYQ"
BASE COUNT          753 a          778 c          767 g          791 t
ORIGIN      
        1 gataaaaatc gtccccgggc tgaggctcaa tttcaaatca gacattttcc gcggacggaa
       61 aataagtaga ttatcatgct gattcggctc ccagctcctg tccctgggat cagaaagcga
      121 gtatgaaaat gctatgttca ccaggttatt tgatgacttc cattcggcat tccatcgtac
      181 ggctgcaact cgggcggctg acaaattccg cggtgaatat ttgccgtcaa acctgcgctc
      241 ccagtaaatc aagtctttta tgtaggagtg ctcgtattca agccccaggt tccatcttcc
      301 ggcgcatctg aaaagaaagc gcagcccggt tctaaaatcc tcggactttt ccggcttaag
      361 atccggattg ccgaccgaga aggcatcctc actccagaaa agcgaagcat aggtgggagc
      421 gcgataggag ttgctgtaag aaaaatgtaa catcaattta tcacttctgc ctttttccag
      481 atcgactctt gccaggggcg aaatcagcgg ccggaaaagt tctgacgctg tctgcgagaa
      541 ggccgctttc acgttgagaa aatcgaagaa cagccgcgca tcattaatcc ccagtcttcc
      601 attataagtc aggttggatt ccattctgtc ttcgccaacc tcaatcggct cctgctcatg
      661 accgagcata tatttctgct tgaaacggtc gaaattcaaa ctcagcctgg ctgaaagaaa
      721 gtggtcagga tcaaatatat acctgggatt cagattaaaa ctcaacctgc tgtcacgata
      781 tttgctgtcg tagggtatga agctgtcata gtccgtaaaa tgctgatgcg aaaggtaata
      841 ctgcgtggaa gcggacagct ttacattttt caaaggagaa agttctgtgc caaggttcca
      901 tgtccggaac gattctgatt ttccggctgt gggggtgaga gaatacatgg cgcccggtaa
      961 tcccgcccgg gagccgacat ccgaaaaggt caggtataca ttatcgtgcc gattgatttt
     1021 gtagttaagg ctggcaaacc tgctgtcaag atctcgatag ttgttctggc gcatactctc
     1081 gcccgccttg ggatcgttat acttgaaatc atttgaggcc gtatttctag agaacacaaa
     1141 aagcccggat aatttttgat gaaagatgtt ctggatcgtc atattggtct cgtcgaaatt
     1201 gaagcttccg aggctcttct cgaagccaat ttctccctca aactccgctt tgcgggtaac
     1261 aatatttaca gcccctgcca atgacccggt gcccatctcg gcgcctgcgc catcggtata
     1321 aatctcaatc tgctttacag cttgtttggg tatgctgttt aagtcgaact cccctgtgag
     1381 acgggaattg accggcatac cgtcaataaa aatccctgta tgcttggcgg gggctccggc
     1441 aatggatagc gaggcggtct gattcgttcc gccggcccgg gaaacaataa tctggggcat
     1501 aaattccagt atctccgcga cagtgcgggc gtttctgaat tccggcgaac tgtcagcgat
     1561 gacgaccttc ccggcagctt ctccatcaga agccgccaca acgatctggt ccggatcata
     1621 atagactctt ttttcaagag cgaaatcctg cctggttgag agcgccgcct caagcgatac
     1681 cttttccgat ctgacggatt cgtatgcctc ggcgtgaact tcaatggtca ccgattcata
     1741 tttgagatta tagatctggt agcggccgtc cttgtcggtc ctgacaatac gcccttcggg
     1801 gactatctgc accgtggcat tctcgatcgg cagtccagac tcaaggtcag tgatacagcc
     1861 ccagagcgaa agaccggacc gtgcgaaaac cgcgatcggt ataagtatga gcaacgtcag
     1921 aaataaaaat gccggtttga atcccggcga aagaagcttt gcagttttat cggtcataaa
     1981 gacttccctc gcccacgcaa ggtttatatt tgccttccat aaccggcagg tctcctgact
     2041 tcacggcatc aaacccggag tttaaagcct tcccatctga tgacagtggc tctcccgcag
     2101 atctgcggtt caaactccag tagcgcgcat acagtagcgg ggctgtggcg gactctcacc
     2161 gccttccctg ttaggctgaa tgcaccggtt atatatcttc aagattaaaa tatgggctga
     2221 ttgcccagtg tcaagcaaaa acaactattg atattctgcg gcaagctgac cgcaggcggc
     2281 tttgatgtca atacctttgg aggcccgtat ggttaccgcc ggcgtgcgcg ggtaaaggta
     2341 atcccggaat ctgtttatgt cattttccgt ggaaggccga aactccgagg gcagcccggg
     2401 agagggattg taattgataa gattgatttt gcatgggata ccgtccacaa atttgaccag
     2461 cttttttgct gcgttgattg tatctgtgat gccggatatt agaagatact cgaaggtcac
     2521 ccgcctgcgg ctttgcgagg tatggtaagt caatgatttt ttcaattcat cgagggagta
     2581 ttttctggta atcggcatca gtctgcgcct ggtttcggga tcggccgagt tcagcgacac
     2641 cgccagcctg accttgaggc cgtcgtcggt catgcggtat atcccgggaa catgaccagc
     2701 cgtggagatt gtaattttgc tgtggccgat tccaaacgca agcactgaca tgataatttc
     2761 gacagcagtt ttgacgttat cgtaattcag aaatggttca cccataccca tgaaaaccag
     2821 gttggaaatc cgttcctccg gctcgagcgt cttctgcaca tgggccagtt gcgacaaaat
     2881 ctcacccacc tccaggttgc gcttgaatcc gatctgagcg gtcttgcaga acttgcaccc
     2941 caggggacat ccgacctgcg aggacatgca caaagtcagg cgcggatggt ccgggatttt
     3001 aaccgcctcg atcaggttac cgtcctcgag ttcaaaaaga tacttcactg tttgatccct
     3061 ggagactttg acctcgagca ctttaggaa
//
LOCUS       DSEB01000232            1749 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1013609, whole genome shotgun sequence.
ACCESSION   DSEB01000232 DSEB01000000
VERSION     DSEB01000232.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1013609"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..>1749)
                     /locus_tag="ENO07_04100"
     CDS             complement(<1..>1749)
                     /locus_tag="ENO07_04100"
                     /inference="COORDINATES: protein motif:HMM:PF04453.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HDS01181.1"
                     /translation="LFTESRLNARKITFLFEDEELTQIQSAGNSYSEYIPVAGDSLVG
                     GRNVASGDSIKLFFDNRNLNEVEVITSVEGRYFSSAEYDSAGGLISEDTILYNSEKLI
                     YNIDGQIINLYDSARVIHETVTLEADTIRYGTESKVVRAESFWEVPPAGDSTYHPVVL
                     RDRNDVIYGERLAYNVDTRRGKIKESDTQLEEAYYHGNIIRKEDEDVLFVEGGRYTTC
                     ELDDPHFHFYSSDMKLITDDRVVAKPIVLYIDKIPVFYLPYFVFSIKKERHSGFLPFQ
                     IGNFERGSRFVNRLGYYWALSDYFDLETSIDYNDEVGVTFNLGFRYALRYKFSGSVDA
                     SYARETQHTLSGLNRRDRWRLNLSHRHTLTETASLNGNGNFVSDASYYTDVSTDPEER
                     LNRSLRSQLNFRKSWGRASVTAVVQGTNNLDQNNTTYNLPRLSFSLPSRAVFSAEDPS
                     EKRWYQNLMLSYSANSDNFISNSGSGEERTSRHYANIRHNISMSMPTTILKYLTISPN
                     ATINENWFYIFDTDQSRAANLMTETGLRRAALTFGMSSNTKLYGMFGPPISGLIAMRH
                     VVTPTLSYSYRPKSDRH"
BASE COUNT          433 a          461 c          406 g          449 t
ORIGIN      
        1 atgacggtcc gatttcggcc gatacgagta ggaaagcgtc ggcgtaacca catgccgcat
       61 tgctatcaga ccgctgatgg gcggcccgaa catgccatag agcttggtgt tcgaactcat
      121 gccaaaggta agtgcggcac gccgtagacc tgtttcggtc atcagattgg ctgccctgct
      181 ttgatccgta tcgaagatat aaaaccagtt ctcatttata gtcgcattcg ggctgatcgt
      241 caaatatttc agtatcgtgg tcggcattga catgctgata ttgtgccgga tattggcata
      301 atgtctgctg gtcctttcct cgcccgatcc gctgttgctg ataaaattat ccgagtttgc
      361 actatacgac agcatcaaat tctggtacca tcgcttttca gaaggatcct ccgccgaaaa
      421 tacggctcgc gaaggaagcg agaatgagag tctgggaagg ttgtaggtgg tattgttctg
      481 atcgagatta ttggtaccct gcaccacggc tgtgacggag gccctccccc agctttttcg
      541 gaagttcaat tgtgaacgca gtgaacggtt cagcctttct tccggatcgg tggagacatc
      601 cgtgtaatag ctggcatcgg aaacgaaatt accgttaccg ttcaggctcg cggtttctgt
      661 caatgtatgc ctgtggctga ggttaaggcg ccacctgtcc cgccggttga gtccactcaa
      721 ggtatgctgt gtttcacggg cataggaggc gtccaccgag ccggaaaatt tatatctcaa
      781 agcatacctg aatcccaggt taaaggtgac ccccacctca tcgttgtaat cgatactagt
      841 ctccagatcg aaataatcgc tcaaggccca gtaatatccc agcctgttca cgaatcgcga
      901 accgcgctca aaattgccga tctggaatgg aagaaaaccc gagtggcgtt ccttcttgat
      961 cgaaaacaca aaatagggca ggtaaaatac cggtatttta tcaatataca acacaatcgg
     1021 cttcgccacc acacgatcgt ccgtaatcag tttcatatcg ctggaataaa aatggaaatg
     1081 cggatcatcc agttcacagg tcgtgtacct tcctccttcg acaaagagca cgtcctcatc
     1141 ctccttgcga ataatattac cgtgatagta ggcctcctcc agctgagtat cagactcctt
     1201 gattttcccc cgacgagtat ccacattata agccagacgc tctccgtaaa ttacatcatt
     1261 cctgtccctg agcacgaccg gatggtaagt tgaatccccg gccggcggga cctcccagaa
     1321 tgactccgcc ctcacaacct tggattcggt gccgtaacgg atcgtatccg cctcgagggt
     1381 cacagtttca tgaatgaccc tggcgctgtc gtacagattt ataatctgcc cgtcaatatt
     1441 gtagatcagc ttttccgaat tgtataagat cgtgtcctcg gagattaatc ccccggcgga
     1501 atcatattcc gcggatgaga aatacctgcc ctcaacggaa gtaattacct ccacctcatt
     1561 caaattcctg ttgtcaaaaa aaagcttgat tgaatcgccc gaggccacat tcctgcctcc
     1621 caccaggctg tccccggcaa cgggaatata ttccgaataa ctgttgccgg cagattgaat
     1681 ttgagtcagt tcttcatcct cgaatagaaa tgttattttc ctggcattca gccggctctc
     1741 ggtgaacag
//
LOCUS       DSEB01000233            4446 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001722, whole genome shotgun sequence.
ACCESSION   DSEB01000233 DSEB01000000
VERSION     DSEB01000233.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001722"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1330)
                     /locus_tag="ENO07_04105"
     CDS             complement(<1..1330)
                     /locus_tag="ENO07_04105"
                     /inference="COORDINATES: protein
                     motif:HMM:PF01743.18,HMM:PF13735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDS01182.1"
                     /translation="MQGLSKKVVDEILKHGNLYEVGGCVRDSLLGMGIAEKDRDYLVT
                     RIEFERLVKVLKKYGHVDLVGKSFGVIKFTSCNNGSDHNVTYDIALPRSEFSTGIGHR
                     DFQVDFDHTLPVEEDLKRRDFTINAIARNLRDNELVDPYGGQEDLKHGLIRMLAPRTF
                     EDDPLRMLRAVQFAARFEFEIEPQTFEAIRQNAGLISAVSPERIQEELNKLLTKAKKP
                     SRGFILMDQTRLLKIILPELALAIGVDQPGGYHAHDVFLHSIYTVDELPQDNLTLRLA
                     GLLHDIAKPRTKIVRDEKANFYGHDQQGAEMAAEILQRLRYSNQVIKDVKVLIKEHMF
                     TSEMSDKGVRRLIKRTGIDRIYDLLELRRSDVRAQGMGNTTPEIDELEGRIRSELEEQ
                     VPLGVKQLAIDGNDIMQEFDIPPSPLVGEILSHLLEIVLDYPEKNNKEVLI"
     gene            complement(1333..2283)
                     /gene="arcC"
                     /locus_tag="ENO07_04110"
     CDS             complement(1333..2283)
                     /gene="arcC"
                     /locus_tag="ENO07_04110"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDS01183.1"
                     /translation="MSPKKQTAVIALGGNAITKKGVPDTIANQFANTRQSLSGIIPLI
                     KNGYNIAITHGNGPQVGNALLRVELARNKAPILPLGICVADTEGGMGYMIEQSLQNRL
                     RKERIKRDVVTIVTQVIVDKDDPSIRNPSKYVGQFYDYNTARKYAKERGWQIKKDANR
                     GWRRVVASPIPIEIVEARQIKILVDKRVIVIAAGGGGIPIYVEENGDFEGVDAVIDKD
                     RASAVLAKSIGAELLLILTGVDNVALNFGKLDQKLLGEVRYKEMKKYLEQGHFPPGSM
                     GPKVEAALGFLEMGGKKVIITSIENAYEAVIGNAGTTITM"
     gene            complement(2283..3605)
                     /locus_tag="ENO07_04115"
     CDS             complement(2283..3605)
                     /locus_tag="ENO07_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HDS01184.1"
                     /translation="MTKNVIIMGAAGRDFHNFNTFYRDNKSYKVVAFTATQIPNIEGR
                     KYPAKLAGKLYPRGIPILPEEDLEKLIAKHKVDEVVFSYSDVPQQHVMHAAAVVDRAG
                     ASFVLLGSKETMIKSKKPVIAITAIRTGCGKSQTTRRVCAVLKEMGKQPVAIRHPMPY
                     GDLSKQISQRFAKLSDLKKHNCTIEEMEEYEPHIKAGTVCYAGVDYAEILKNAEKEAD
                     VIVWDGGNNDTPFYKPDLWIVVTDPHRAGHELTYFPGFTNFISADVIVMNKMETADPE
                     DIEELRENISIFNPLATVIEGVSPVFVDDPEVIRGKSVLVVEDGPTLTHGEMRYGAGI
                     VAAQKFGAIDFVDPRPFLKGTLKSTFEKYPNIGPLLPAMGYGDQQIKDLSATINATEC
                     DSVVIATPIDLRRIINIKKPSTRVSYELQEIGAPDMTMVISEFFKKKK"
     gene            3900..>4446
                     /locus_tag="ENO07_04120"
     CDS             3900..>4446
                     /locus_tag="ENO07_04120"
                     /inference="COORDINATES: protein motif:HMM:PF06089.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDS01185.1"
                     /translation="MPMQEILAEVYRGKGIESVHFGSVVVVDNRGQVQYYAGDPDLVS
                     FTRSSLKPFQAVPLTERGGLEKYGFDLKALAIMCGSHSGSPIHTEQVKKNLDKIGLDE
                     SYLQCGTHPPIYYTSKNILPRREETFTPIQHNCSGKHSGQLALALMLGCDPKRYLDPN
                     YEPQKLVKQTVSEICEVSPDDM"
BASE COUNT         1057 a         1157 c         1044 g         1188 t
ORIGIN      
        1 gtattagtac ttctttatta tttttttcag gataatcaag gacaatttcc aaaagatgcg
       61 ataatatctc ccccaccagc ggcgagggcg ggatatcgaa ctcctgcatt atgtcatttc
      121 catcgatcgc aagctgttta actcccagcg gaacctgctc ctccagctcg cttcgaatcc
      181 gtccctccag ctcatcgatt tccggcgtcg tatttcccat cccctgggcc cggacatcgc
      241 tccggcgcag ttccagcagg tcatagattc tatcgatacc ggttctcttt attaggcgac
      301 ggacgccctt gtcggacatc tccgaggtga acatatgttc cttaatcaag acctttacat
      361 cttttatgac ctgatttgaa tagcgcagac gctgcaggat ttccgccgcc atctcggctc
      421 cctgctggtc atggccataa aaattggcct tttcatcccg gacaatcttt gtccggggct
      481 tggcgatatc atgcagcagc cctgcaagcc tgagtgtcaa attgtcctgg ggcaattcgt
      541 cgacggtgta gatagaatgc agaaaaacat catgggcgtg atatcctccg ggttgatcca
      601 cccctattgc gagcgccagt tcaggcagaa taatcttgag taacctagtc tggtccatga
      661 gaataaaacc ccgggaaggt ttttttgcct tggtcagaag cttgttcagt tcttcctgga
      721 tacgttccgg agagaccgcg ctgatcaatc cggcgttctg gcggatcgct tcaaatgtct
      781 gcggttcgat ttcgaattcg aaacgggccg cgaactgcac cgccctgagc atgcgcaaag
      841 gatcgtcctc aaaggtgcgg ggagccagca tgcggatcag gccgtgcttc agatcctctt
      901 ggcccccata aggatcaacc agttcattat ccctgagatt gcgggcaatg gcattgatgg
      961 taaaatcacg gcgtttcaaa tcttcctcaa ccggaagagt gtggtcgaaa tcaacctgga
     1021 aatcgcgatg accaatgccg gtggaaaact cgctccgcgg caaggcaatg tcataggtga
     1081 cattatgatc cgagccgtta ttgcaggagg taaattttat tacaccgaat gatttcccga
     1141 ccagatcgac atgcccatac ttcttcagaa ccttgacgag tctctcaaat tctatcctgg
     1201 tcacaaggta atcgcggtct ttttcggcaa tacccattcc cagaagggaa tcgcggacac
     1261 atccgcccac ctcgtagaga ttcccatgtt tcaggatctc atccacaact tttttgctca
     1321 ggccctgcat gattacatgg ttattgtggt accggcattg ccgataaccg cctcatacgc
     1381 attttcgatt gaggttatta taactttctt gccgcccatc tccaaaaatc caagggctgc
     1441 ttccactttg ggtcccatac ttccgggtgg aaaatgcccc tgctccaaat atttcttcat
     1501 ttctttatat ctaacttctc ccagcagctt ctgatccagc tttccgaaat tcagggcaac
     1561 attatccacg ccggtcaaaa tcaagagcag ttctgccccg atggatttag ccagaaccgc
     1621 acttgcccgg tccttatcga taaccgcatc gacgccctca aaatcgccgt tctcctcgac
     1681 ataaatagga attcctcctc ctccggcggc tattactatc acccgtttat ccaccaggat
     1741 tttgatttgc ctggcctcga caatttcgat cgggattggt gaagccacca cccgtcgcca
     1801 gccgcggttg gcatcttttt ttatctgcca gccccgctct ttggcgtatt tgcgggcagt
     1861 gttgtaatcg taaaactggc ccacatattt gctgggattt cttatggagg gatcgtcctt
     1921 atcgactatc acctgggtta ctatggtgac gacatcgcgc ttgatcctct cttttctgag
     1981 acggttctgg agggactgct cgatcatata gcccataccg ccctcggtat cggccacaca
     2041 gattcccaaa gggagaatgg gggctttgtt ccgggccagt tcaacacgca gcagagcatt
     2101 gcccacctgg ggcccattgc cgtgagttat tgcgatgttg taaccgtttt ttatcagcgg
     2161 gataatacca gatagtgatt ggcgtgtatt cgcaaattga tttgcaatgg tgtcgggaac
     2221 gcctttttta gttatggcgt tcccgcccaa tgctattacc gctgtctgtt ttttaggaga
     2281 cattatttct tcttcttaaa gaactccgag atgaccatgg tcatatcggg ggcgccgatc
     2341 tcctgcaatt cgtagctcac cctggtcgaa ggcttcttga tatttatgat gcgccttaaa
     2401 tcgataggag tggcgataac gactgaatca cattcggtcg cattgatagt cgcgctcaga
     2461 tcttttatct gctggtcgcc gtagcccata gccgggagca agggcccgat gttcggatat
     2521 ttctcgaaag tgcttttcag agtgcctttc aggaatgggc gcgggtcgac aaaatctatc
     2581 gcgccgaatt tctgggcggc cactatacct gcgccgtacc gcatctcgcc gtgcgtaagc
     2641 gtgggaccgt cctcgaccac cagaacactt ttgccgcgga tgacttcggg gtcatccaca
     2701 aacaccggag agactccctc gatcacagtt gcgaggggat tgaagatcga gatattctca
     2761 cgcagttcct ctatatcctc cgggtcggca gtttccattt tattcatgac gattacatcg
     2821 gccgagatga aattcgtgaa gcccgggaaa taggtcagct cgtggccggc gcgatgagga
     2881 tcggtgacca ctatccagag atcgggtttg tagaatggag tatcgttgtt gccgccgtcc
     2941 catacgatca catcagcttc cttctcggcg tttttcagga tctcggcata atcgactccg
     3001 gcataacaga ccgtacccgc cttgatatgc ggctcgtatt cttccatctc ttcaatcgta
     3061 caattatgtt ttttaaggtc agacagtttt gcaaaacgct ggctgatctg cttggaaaga
     3121 tcgccgtagg gcatgggatg acggattgca accggctgct tgcccatttc cttcaagacc
     3181 gcacaaaccc ttcgcgtggt ctggcttttg ccgcacccgg tccggatcgc ggtgatcgca
     3241 ataacaggct ttttggattt gatcatggtc tccttggagc ccagaagcac aaacgaagct
     3301 cccgcccgat cgacgactgc ggcggcatgc ataacatgct gctgcggaac atcagaatag
     3361 gagaaaacca cctcatcaac tttgtgtttt gcgataagtt tttccaggtc ctcttcgggc
     3421 agaatcggta tgcccctggg gtatagcttg ccggcaagtt tggcgggata ctttctgcct
     3481 tcgatattgg gaatctgtgt ggccgtaaag gcgaccactt tgtacgactt attatccctg
     3541 taaaaagtgt tgaagttatg aaaatctctt cctgccgcac ccatgataat tacgttcttc
     3601 gtcattaaat cctcctatta aatcatagaa atagatggct tgttgagtag ctgactgata
     3661 acacaggcac agtcagatcg aggacatgtt ccagtagtgc acaacaagat aataatcaaa
     3721 acgcataata tcatctcacc ccatcttaaa ttagtaaaga gaatctatca gaatgcgcaa
     3781 gaatttcaag cactttttga catttttttg aataaacaat tcgtttaatt tgacttataa
     3841 aaatagcctc aaaagaccat aaaataacgg ttgaattata atcggctgtt tgttataata
     3901 tgccgatgca agagatactg gccgaggtat acagaggaaa gggcatcgaa tcggtccact
     3961 ttggatcggt cgtggtggtc gacaacaggg gccaggtgca atactatgcc ggagaccctg
     4021 atcttgtgtc cttcacgcgt tcatccctga aaccattcca ggccgtgcca ttaacagaga
     4081 gaggcgggct ggaaaaatat ggattcgact taaaagctct tgccatcatg tgcggttccc
     4141 attcaggcag cccaatccat accgagcagg taaagaaaaa tctggacaaa atcgggcttg
     4201 acgaaagtta tcttcaatgc gggacacatc cgcccatcta ctataccagc aaaaatatac
     4261 ttccccggcg ggaggagact ttcacaccga tccagcacaa ctgttccggc aagcattcgg
     4321 gacaactggc gctggctttg atgctgggct gtgacccgaa gcgttatctc gatcccaatt
     4381 acgagccgca gaagctggtt aagcaaaccg tctcggagat atgtgaggtt tctcctgatg
     4441 atatga
//
LOCUS       DSEB01000234            4443 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001723, whole genome shotgun sequence.
ACCESSION   DSEB01000234 DSEB01000000
VERSION     DSEB01000234.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001723"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..554)
                     /locus_tag="ENO07_04125"
     CDS             complement(<1..554)
                     /locus_tag="ENO07_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01186.1"
                     /translation="MKRSLFLTFLMLLLTFGFATAANRIVTPPFGDMMGDPYSLNNAG
                     LTGVTVYWQFENDHIIIGWSNGFSVEATGNVTASIVNGSGNYVVGSRFEPIFNVQGMP
                     WNWYYEPTGDTIGYGGVWTPPNTFPGFATGPLADACYMDLDITLTGGTPETNTGTICV
                     DSVIKFGPAGDWLWDGWDGETSPPV"
     gene            complement(816..2009)
                     /locus_tag="ENO07_04130"
     CDS             complement(816..2009)
                     /locus_tag="ENO07_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01187.1"
                     /translation="MASGPVNKLLCLVLLTVVLILFGPVDGNGQLITYIDSVETQPDN
                     DSIVINMKIDNFSGDKITGFTLWISLQYADFIGFYADSGWLVDTTYETCLEDSCIFYD
                     YVGDSCLLSTCLAWADTTIDSSWVEKGAVDTAGSLTAGWDFMDVAILDNENKNIKVNA
                     IANPPSGDPVNGIPSPTSQGLLCRIRAEVKPYTIYDSCEVQDGDSTIMLPWEECFCDS
                     IIRITFDTAQTRFSNDSGQFIGWVWSDTAYACATVPPDLHKECVRKCASDELNNYCLD
                     SVCYYEYGGSCYLWECTNCLLWDSTGYVDPSQVLFSHGQIEVLCPEYLCGDANADGSA
                     NVSDAVWIINYVFVGGPPPEPLSSGEVNCDGSVNVSDAVWIINYVFVGGGIPCDINGD
                     GIPDC"
     gene            complement(2981..4174)
                     /locus_tag="ENO07_04135"
     CDS             complement(2981..4174)
                     /locus_tag="ENO07_04135"
                     /inference="COORDINATES: protein motif:HMM:PF00465.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDS01188.1"
                     /translation="MESFVFRNPVKIIFGVGEVKKAGEEAAGIGKKALIVTGQSHVKK
                     SGLFDRIAGYLTDAGVEFVHFEGIEPNPRAATIDRAGEIARQNDCDMVLGVGGGSTMD
                     ASKAIAVVTRSAHPIWDYVYNEDSEKKREVEEALPIMLIPTVAATASEGNRGGIITNW
                     ETNQKAGLFSDHMYPKVSIVDPELTATLPRQTTIDGAIDIISHVIESYFTGPDNTPLQ
                     DRFSEGIMRTVMENLEILLEEPGNLDARATMSWCSTMALLGPVNLGRPGPFPLHFIEH
                     VISGHYDISHGRGLAIMLPPLMRYTFRDRPEKYAQLAKNIFFIDTDKMDLQKAAETFI
                     SRFEDWMKKVGMYARLSDEGIGDERLEKMVEDTIEVYGGGKDHIGSYRPLYRDDLAAI
                     FRMGL"
BASE COUNT         1223 a         1151 c          919 g         1150 t
ORIGIN      
        1 actggaggag aggtttctcc atcccagccg tcccagagcc agtcacctgc aggtccgaat
       61 ttgatgaccg agtcgacaca aatcgtgccg gtgttagtct ccggagtacc tccagtcaag
      121 gtgatatcca gatccatata acaggcatca gccagggggc cggtggcgaa accagggaag
      181 gtgttcgggg gtgtccagac accgccgtac cctatggtat cacccgtagg ttcataatac
      241 cagttccagg gcatgccctg cacgttgaag atcggctcaa aacggctgcc tacaacgtaa
      301 ttgccggatc cattgactat ggaggcggtg acgttgccgg tggcctcgac tgagaaaccg
      361 ttcgaccaac caattataat atggtcattc tcgaactgcc agtagaccgt cacacccgtc
      421 agacccgcat tattgagaga atacggatcg cccatcatgt caccgaaagg cggtgtcacg
      481 atacggttcg cagccgtagc aaaaccaaaa gtgagaagaa gcatcaaaaa ggtaagaaac
      541 aaactgcgct tcatagtaaa ctccttaact aaagaagtat taggttaata ttgttttttc
      601 tacctttccc ccctcaagga aaactagttt ggcttgtatt gctgaatttc agaaattttt
      661 cgtccattca gcggtggctt gaggttacag ggcctcttct tcttcttctc tttccacctc
      721 ctttcttttc ctaaatgaat tacctttttg tatctcacta aaggtaatta cttcctaagt
      781 aataaaatat gatcagcaaa aggttttttc atagttcaac agtccggaat accgtcccca
      841 tttatatcac agggtatccc ccccccgaca aagacgtaat tgataatcca gacagcgtcg
      901 gagacattga ccgagccgtc acaattgacc tccccactgg acaatggttc gggcggcgga
      961 cctccgacaa agacatagtt tattatccag acggcatcgg aaacattggc cgagccgtcc
     1021 gcatttgcat ccccgcataa atattcggga catagcacct caatctgccc gtgcgagaac
     1081 aaaacctggc tcggatcgac atatccggtc gaatcccaca agagacagtt tgtgcattcc
     1141 cagagatagc aggaaccccc atattcatag taacataccg aatccaggca gtagttgttt
     1201 aactcgtcgc tggcgcactt gcgcacacat tccttgtgca ggtcaggcgg tacggtggcg
     1261 caggcataag ctgtatcact ccagacccag ccgataaact gccccgagtc attcgagaat
     1321 ctggtctggg cggtatcaaa tgtaatcctt ataatagaat cacagaaaca ttcctcccag
     1381 ggcagcataa tagtgctgtc tccatcctgc acttcacaac tgtcataaat cgtataaggc
     1441 ttcacttcag ccctgatcct gcataaaagt ccctgcgaag tgggcgatgg tatgccgttc
     1501 accggatcgc cactgggcgg attggcaatc gcattgacct ttatattttt gttctcgttg
     1561 tcaagaatgg caacatccat aaaatcccat cctgcagtca gtgaaccggc agtatcgaca
     1621 gcgcccttct ccacccagct tgaatcgatc gtagtatcgg cccatgccaa gcaggtcgac
     1681 aaaagacaac tgtcacccac gtaatcatag aagatacacg aatcctccag gcaagtctca
     1741 taagtcgtat caaccagcca tccggaatcg gcataaaagc cgatgaaatc cgcatactgc
     1801 agcgatatcc agagagtaaa gccggttatc ttatcgccgc taaagttgtc gatcttcatg
     1861 tttatgacaa tgctatcgtt atcaggctgc gtttccacag aatcgatata tgtaatcaat
     1921 tgaccattcc catcgacagg accgaataat ataagtacaa ccgtaagtaa tactaaacag
     1981 agcagtttat ttacaggtcc gctggccata aatctcctcc ttaattaatg attgggagcc
     2041 ttagttttaa ttcccgtagc aatcatgtac aatagtaata tcgcctccat tgccgacttg
     2101 gtttagcttt tcgatatccc aatcattaca cgagtatgat aatggcctcg tttttaagta
     2161 ttctcgatga agtaacttcc actcaaaatg ttcagtactg gttgatagag cccagaaact
     2221 ctgttttctt tcgagacaaa agtttcagtt tacaactgta tcaacgaaga ctcaatacat
     2281 ccgccaggta acgtaccaaa atgagtattt tgtgaaaggt tgaggattaa ttcttgtcca
     2341 taattaaatt ctgtcgggct aaatataagt ccgcttgcgt cttttgtcaa cagaaaataa
     2401 atcctttttt gaatattttt gtttgaccat tttattgcaa tccagacgat tcaggcgcta
     2461 ttttaagcca aatcgactcc tctaaggtga ccttccaaaa agcaagtaat atgccagctg
     2521 aaaaaagagc cattaagtaa ttggtacagg tgtatgatac aaaaagcagc tattttcgca
     2581 atatcttttt tgaaaaaaat aaaaaaggcc gaaaaactga ccggttgctt gagagggggg
     2641 cataaactca aactggaccg atcagccctc agcccgtata ttttccaaag tatgaaacat
     2701 atcaaacatt gttttgttcc ttcttttctt caaatatact tcgcatggca ttctgccgcc
     2761 ggatacggct ttgataccat aaaagaccca atctattgca ctctatacat ttacaagcaa
     2821 tacccctcca gatacgattc cccgccctga ccgacaatcg ataaatccgg tcataacctg
     2881 gaaaatggag caaaaatatg aatcattttg caaaaaagtt gaaattgacc acctgaaatt
     2941 ccaagaggca gagacgtcct gaccggattt tgaagcaatc ctacaggccc atccggaata
     3001 ttgccgcgag atcatctctg taaagcggcc tgtaacttcc aatatgatcc ttaccgccgc
     3061 cgtaaacttc gattgtatct tccaccatct tctccagcct ttcatccccg attccctcgt
     3121 cagaaagtct ggcatacatg cccacttttt tcatccaatc ttcaaaacgg gagataaagg
     3181 tctcagctgc tttctgcaga tccattttat ccgtgtcaat aaagaagata tttttggcta
     3241 actgcgcata tttttccggg cgatcccgga aggtatacct cataagcgga ggcagcataa
     3301 tcgccagccc gcggccatgc gatatatcgt aatggcccga gatgacatgt tcgatgaaat
     3361 gcagcgggaa tggccccgga cggcccaggt tcaccggccc gagtaaagcc atagtcgagc
     3421 accaggacat agtggcccgg gcatccagat tccccggctc ctccagcaga atttccagat
     3481 tctccatcac tgtccgcata atgccctcag aaaaccggtc ctgcaggggc gtattgtccg
     3541 gcccggtaaa ataggattcg atcacgtggc tgattatatc aatggcgccg tctatagtgg
     3601 tctggcgcgg aagcgtagcg gtaagctcag gatccacaat ggaaaccttt ggatacatat
     3661 gatcggaaaa aagacccgcc ttctggttgg tctcccagtt cgtaattatt ccgccccggt
     3721 tgccctcgga ggcagttgca gccacagtcg gaatcagcat aatgggcagg gcctcctcaa
     3781 cttcacgttt tttctcagaa tcttcattgt aaacatagtc ccagatcggg tgcgcggatc
     3841 tggtaaccac agcaatcgcc ttggaagcat ccatggtcga tcctcctccc actcccagca
     3901 ccatatcgca gtcattctgg cgcgcgattt cgcccgcccg gtcgattgta gcggcacggg
     3961 gattgggctc gattccttca aaatggacaa attcgacccc tgcatctgtt aaatatccgg
     4021 cgattctatc aaacaaaccg cttttcttaa catgcgattg cccggtaaca ataagcgcct
     4081 tcttcccgat tccggcggcc tcttccccgg cctttttgac ctcgcccacc ccgaatatta
     4141 tcttcacagg atttctgaat acaaagcttt ccatgcgcag ctccttttta ttggtcctgt
     4201 ccacacagga ttctatgttc taaagaatct ataagataca ttatttcaac catttcacaa
     4261 cagatagaag actgaaatgt tttggcaaaa gtaaaaaaac cgccacttca gaaagcggcg
     4321 gtcatattgc tgtcgaacca agggagtagt gcagataatc ttataattct aactctcctg
     4381 ggaggaaatc tctgccatcg ctcttccttt cttgctgtag aagagcctgc tgataaccat
     4441 ctc
//
LOCUS       DSEB01000235            7997 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000488, whole genome shotgun sequence.
ACCESSION   DSEB01000235 DSEB01000000
VERSION     DSEB01000235.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7997)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7997)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7997
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000488"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="ENO07_04140"
     CDS             complement(<1..1185)
                     /locus_tag="ENO07_04140"
                     /inference="COORDINATES: protein motif:HMM:PRK00286.2-3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDS01189.1"
                     /translation="MEAEILTVTQLTDLIKGTLEETFPGLWVTGEISNYLHHSSGHRY
                     FTLKDPGAQIKCVIWKFVGQHLTFEPENGMKVKLFGDVTVYSRGGVYQIRVTRLLPLG
                     VGELEAKFQELKQKLYEEGLFDEEHKKPLPLFPSTIGIVTSPTGAAIRDMINILTRRA
                     PWVRIILAPVHVQGEGAAEEIARAIGNFNRFKAVDLIIAGRGGGSLEDLWAFNEEVVA
                     RAIYASDIPVVSAVGHQIDYTISDFVADLRAPTPSAAAELVAPDIIDLRAALRNMRLK
                     LSRGAMAYIELAREKLSGLTRSPVFRRPMEFIYARQQEIDDFKMALQKSMTAYMKYQR
                     QELSSMTEKLVALSPEGILQRGYAVVRSIPDGKILKRAEETSEGKAVSIKLYKGSLDA
                     TVD"
     gene            complement(1185..2591)
                     /locus_tag="ENO07_04145"
     CDS             complement(1185..2591)
                     /locus_tag="ENO07_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01190.1"
                     /translation="MALSLTIAVEKNEYLPGESIPVEMTLVNITDSVVNIPNFAYNSD
                     LTEIIISDSEGNEIGRLNDPVRQLLLGFMQVEVIKPPEQSLSPGSPQKAEFDVSQYVR
                     LTKAGKYQLQGSFRYDDEQVLSDPVEINILEGKASGLYHKWQYQMGGKWRCQMVYRDH
                     DKLMHMLGYRHNPGVIDYNLPLMDDFTVDEYALAFSCYDKDDYLPWILGVKDKKLVGG
                     KIDTASFVTGLEELEFKGKTFLNAAVETYDRTLILPEVLKDKILFHLFDAEGKKNAQK
                     EIGIGSGTEVLDASAYKSGEYLMVYRETTSKGSDIYAAVSKKADLSDMEQRKLVGSSK
                     EFDAAVISPTVKMPSDFYAVSFEPEEKALMIYTVSLNPDIRTQVPPKVILKSQGELQL
                     HSFAVEQNGAAYVLFIEDGKKLLYYNVSDNSFEFITEEEFSDPQLIITESDGVFLGYI
                     DHDGIIQFKHMEKVIKRH"
     gene            complement(2635..5070)
                     /locus_tag="ENO07_04150"
     CDS             complement(2635..5070)
                     /locus_tag="ENO07_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HDS01191.1"
                     /translation="MQIGLPGVVIEGIDEQFAPHQEQLEIRCRFIDIHNEIQGARLKV
                     YASNYEDSNYPDDGALVYSEDIVTDDRPHNVIRTFRWYGNTTAEAGALQQPGEDLIYV
                     NPLFAPYTVEIKISTANEDPDSDSDAKINYKRILEDGSEVDWQFKVEYHSIEFELGEH
                     IDLENAPDPQSTAWVQWRLNELGYPAGPVDGTGGDMTERAVKNFQRAHWQVPWPEDEN
                     ADRTPLVVDGNAGDNTMAVLESEQVEQRELFSSVDDLFDPGAGVKLYSWANIFYQYQS
                     NVEDGEQEWDDSNRDEWWDSRFSPRMFPRHDKEELVLNRPWIPIVAKLMIKNRSGDPV
                     FCPQGVGNVRINFIIRDPSEDMEVTSTDAISTPRQYVDHARQEVDTATGDNCPEPHEG
                     IRVTDEGENYKKYIHVGDALEPWEGQDDSSNHVVYVECPTQGRGRGMAGIYFIPSYQA
                     GDNFKIRAQMELTGHPNRENIEPADGPLSAESATITNWRKMKVAAFLRFCTRAAAGED
                     EPSLADRITSGLGEFENAYIEFEDPAFNESVTEFLTEEEYLDVVCTAYNLTTDEERAT
                     VRFRPNTCLYGFDLREQNDGETPAAYRNFIRSTVETFCDRVMEPLGKVIINNVRKRCR
                     EGSVVIDFQPHAPATVYNNKGAATETVVDNNYRASTYAVGLHNGLVYLDIILNEESGM
                     DFAYLLAHEIGHTVFLYHWENTDTGTLSNAGDYENRVNFRVNADHHDHNDNNCIMSYP
                     LDFGVIPNPPAGLVLDGTPINSVWDYIRYVQAQGATHQRHHQLAINYRPDFCGKCLLK
                     LRGWNVYNLPQSY"
     gene            complement(5478..7268)
                     /locus_tag="ENO07_04155"
     CDS             complement(5478..7268)
                     /locus_tag="ENO07_04155"
                     /inference="COORDINATES: protein motif:HMM:PF05954.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HDS01192.1"
                     /translation="MPRSQTQDQEQQNNQLLTPSFKILVDGQPLDTRIESRVLFIKAE
                     SNLEILDMFEIRFYDFDLELVNSDDLAIGKSVEVKLGYQESQLSTVADCEVVSLEPEY
                     PPGGAAYLTIRGYDKSFRLSREKKSRSFVEMKDSDIANQIAREMGLSAHVDATTEVHP
                     YVFQRNQANLEFLLERAQRIMYEMYIEGNDLYFRRPQSDQARRVTLKWGESLASFSPR
                     LSSFNQVSEVVVRGWDPKSKKEIVGRAGGGDEHTTLGGAQTATQIAEAAHGSTKSFKV
                     NKPIHSQAEADALARAHFNKLSMNFITGEGKAPGEPELKAGTVIDIEGLGDKFSGSYY
                     VTRATHIFSASGYSTKFEVKKNSLGRPPAPPEEQAEEEQEQNQHYLDVTLQDSAEEAV
                     SGLDYIIIAPDGTRYTGSVGSDGKIHKDNLPQGESQIIFKQLANAHWEVDQVNCGDEV
                     RLLVECPAHDEGDSITFEIYKLFKEESGDEITSIDGTVDANSQAEAVWTYEFQEEDEG
                     TKPRFIFKAKSGSLETKSDVLTVVDVFEATLTDSDGNPVANTGYVLILPDGSTREGST
                     DEQGRILEEEVPVGNIKVRLDDGSTLEQSS"
     gene            complement(7272..7973)
                     /locus_tag="ENO07_04160"
     CDS             complement(7272..7973)
                     /locus_tag="ENO07_04160"
                     /inference="COORDINATES: protein motif:HMM:PF13189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HDS01193.1"
                     /translation="MPSIEQIVDRQIRKWELLKEKRENGEVERQLPPPIITVSRMRGS
                     RGSYLAQRLAEELGYQLMHKEIIEHIVNSSGIRRRLIESLDEKYRNQVELWLEGIFKG
                     RYSDASDYFRHLYRAIITLSRLGGVVVVGRGSNFILTIQTGFHLRVVASSAIREKNLM
                     KYENLSQEGARQQIADFDKERQDFIKRNFKRDINDPEYYDMILSTNYIDIEDAVAFIK
                     EAIEAKFTKQKYLAE"
BASE COUNT         1866 a         2277 c         1747 g         2107 t
ORIGIN      
        1 gtccacagtc gcatccagac tgcctttata caactttatg ctgacagctt ttccctcaga
       61 tgtctcctcc gcccgcttca atatttttcc atcaggtata gacctgacta ccgcatatcc
      121 tctctgcaga atcccctcgg gtgaaagcgc taccagtttt tcggtcatgg atgatagctc
      181 ctgccgttga tatttcatat aggcggtcat gcttttctga agcgccatct tgaagtcgtc
      241 gatctcctgc tgacgggcat agataaattc catcggccgt ctgaagaccg gcgatcttgt
      301 caatcccgaa agcttctccc ttgccagttc aatatatgcc atcgcccctc gcgagagctt
      361 gagccgcata ttgcgaagtg ccgctctaag atctattata tccggcgcca caagttcggc
      421 cgcggccgag ggcgtcggcg cacgaagatc tgccacgaaa tccgaaatcg tgtaatcgat
      481 ctgatgtccc accgccgata caaccggaat atccgaggca tatatcgccc gcgctacaac
      541 ctcctcattg aaggcccaca aatcctccag gctgcctccg ccccgcccgg cgataatcag
      601 gtccaccgct ttgaagcgat tgaaatttcc gatcgcccgg gcaatttcct ccgccgcacc
      661 ctctccctga acatgtaccg gagccaggat tatccttacc cagggagcac gccgggtgag
      721 aatatttatc atatcccgga ttgccgctcc ggtgggagag gtcacgattc caatagtcga
      781 tggaaaaagc ggcaatggct tcttatgctc ttcatcaaat aatccttctt catatagctt
      841 ctgtttcagc tcctgaaatt tggcttccag ttcgccgaca ccaagcggca gaagacgcgt
      901 caccctgatc tggtaaacgc cgccgcgaga atacaccgta acatccccga acagcttgac
      961 cttcatcccg ttttcaggct cgaatgtcag atgctgtccg acaaatttcc agatcacaca
     1021 tttaatctgt gcacccggat ccttgagcgt gaaatagcga tgccccgagg agtggtgcag
     1081 atagttggaa atctccccgg ttacccacag gcccgggaag gtctcttcca atgtcccctt
     1141 gatcagatcg gtcagttgcg ttacggtaag tatctcggct tccatcagtg cctcttgatc
     1201 accttctcca tatgcttgaa ttgaatgatt ccatcatgat caatataacc aaggaacacg
     1261 ccatctgatt cggttattat caactgcgga tcagaaaatt cttcctcggt aatgaattcg
     1321 aagctgttat cggaaacatt ataatacagc aatttcttcc catcttctat gaacagcaca
     1381 taagcagcgc cattctgttc aaccgcaaac gaatgcaatt gcagttctcc ctgagatttc
     1441 aatatcacct ttggcggaac ctgggtacgg atatccggat tgagactgac tgtatatatc
     1501 atcaaagcct tctcctcagg ctcaaacgac accgcataga aatcactcgg catcttaaca
     1561 gtgggtgaaa tgactgcggc atcaaactcc ttgctcgatc ccaccagctt ccgctgctcc
     1621 atatcggaca aatccgcctt tttgctgacc gccgcatata tatccgaacc tttgctggta
     1681 gtctctctat agaccatcag atattcgccg gatttatacg ccgatgcatc gagtacttca
     1741 gtcccactcc cgatcccgat ctccttctgt gcattctttt taccttccgc atcaaacaga
     1801 tgaaacaaga ttttatcttt caatacttca ggtaaaatca gagttctatc gtatgtttcc
     1861 actgccgcat tgagaaatgt tttgcccttg aattctaatt cttccagccc ggtaacaaaa
     1921 ctggctgtat cgatctttcc accaacgagc tttttatcct tcacgccaag aatccatggc
     1981 aggtaatcat ctttatcata gcatgaaaac gccagcgcat attcatctac tgtaaaatca
     2041 tccatgagtg gaaggttata gtcgattacc ccgggattat gacgataacc cagcatgtgc
     2101 atgagtttgt catgatcccg atataccatc tgacatctcc atttaccacc catctgatac
     2161 tgccatttat gataaagacc cgaagccttg ccctcgagga tattgatctc caccggatct
     2221 gacaaaacct gctcatcatc ataccggaaa ctaccctgca attgatactt tcccgctttt
     2281 gtcagacgaa catactggct gacatcaaac tcagccttct gcggagagcc ggggctcaag
     2341 gactgctccg gcggtttaat tacttccact tgcatgaatc ccagaaggag ctggcgcacc
     2401 gggtcgttca gacggccgat ctcatttcct tccgaatcgg aaataataat ttccgtcaga
     2461 tcgctgttat aggcaaaatt ggggatattg accaccgaat cggtgatatt gacgagagtc
     2521 atctctaccg ggatagattc gcccggcagg tactcatttt tctcgaccgc tattgttaac
     2581 gacagcgcca tcttactctc cccgcattga tttacaaaca acagcaaaca taaactaata
     2641 actctgaggc aaattatata cattccatcc cctaagttta aggagacact ttccacagaa
     2701 atcggggcgg taattgatcg ccagctgatg atgtctttga tgcgtcgcgc cctgggcctg
     2761 gacatacctg atataatccc aaactgaatt gataggagtg ccgtccagta ccagcccggc
     2821 aggcggattc ggtatcacac caaagtccag tggataactc ataatgcaat tattgtcatt
     2881 atgatcgtga tgatcggcat tgaccctgaa attcacccga ttctcgtaat cgccggcatt
     2941 gctcaatgta cctgtatcgg tattttccca gtggtacagg aaaactgtat gtccgatctc
     3001 atgcgccagc agataggcaa aatccatacc cgattcttca ttcagaataa tatccagata
     3061 cacaaggccg ttatgcaatc ccaccgcgta ggttgaggct cgataattat tatccaccac
     3121 tgtctcagtt gcagcgcctt tattattata aacagtagcc ggagcatgag gctggaaatc
     3181 tattaccacg ctgccctcac ggcatctctt tctgacatta tttattatta ccttgcccag
     3241 cggctccatc accctgtcac aaaatgtctc cacagtggac cggataaaat tgcgataggc
     3301 cgccggcgtt tcgccatcat tctgctcacg cagatcgaat ccataaaggc atgtgttcgg
     3361 cctgaagcgg actgtggcac gttcctcgtc ggtagtcagg ttataggcgg tacagaccac
     3421 atcaagatat tcctcttcag tcaggaattc ggtcacggat tcattgaagg ctggatcctc
     3481 aaattctata tacgcattct caaattctcc cagaccggaa gtaattctgt cagccagcga
     3541 gggttcatct tcaccggctg cagcacgggt gcagaatctc aggaaggcag ccaccttcat
     3601 cttgcgccag tttgtgatag tggcggattc ggcgctcagg ggaccatctg caggctcgat
     3661 attttcacgg ttgggatgac cggtcagttc catctgtgct cgaatcttga aattatcccc
     3721 ggcttgatat gacggtataa agtaaatccc ggccatgccg cgccccctgc cctgcgtggg
     3781 gcattcgaca tagacgacat gattggaact gtcgtcctgc ccctcccagg gctccagcgc
     3841 atcaccgaca tgaatatatt tcttgtaatt ctcaccctca tcggtgacac ggatgccttc
     3901 atgaggctca gggcaattat ctcctgtagc agtatccact tcctgacggg cgtgatcgac
     3961 atactgcctc ggcgtcgaga ttgcatccgt agaagttact tccatatcct cacttggatc
     4021 cctgattatg aaatttatcc tgacattgcc gacaccctgc gggcagaaaa ccggatcgcc
     4081 gcttctgttt tttatcataa gcttggccac aatcggaatc cagggacgat tcaacaccag
     4141 ctcctcctta tcatgacgcg gaaacatgcg cggactgaag cggctgtccc accattcatc
     4201 ccgattggaa tcatcccatt cctgttcgcc atcctcaaca ttggactggt actggtaaaa
     4261 tatattggcc catgagtata gtttcacgcc ggccccggga tcaaagaggt cgtccaccga
     4321 agaaaaaagc tccctctgtt ccacctgctc cgattccagc accgccattg tattatctcc
     4381 cgcgttgccg tccacaacta atggcgtacg atcggcgttc tcatcctccg gccaggggac
     4441 ctgccagtgg gcgcgctgaa agttcttgac agcgcgctcg gtcatatccc cgcctgtacc
     4501 atcaaccggc ccggccggat aaccgagttc attcaaccgc cactgcaccc aggctgtgga
     4561 ctgcgggtca ggtgcatttt ccaggtctat atgttctccc aactcgaatt caatggaatg
     4621 atattcgact ttaaattgcc agtcaacttc ggatccatcc tccaggatgc gtttgtaatt
     4681 gatcttcgca tccgaatcgg aatccggatc ttcatttgcg gttgatatct ttatctcgac
     4741 ggtgtatggc gcaaataacg gatttacata tatcaaatcc tctccgggct gctgcaatgc
     4801 cccggcttcc gcggtggtat tgccgtacca tctaaaagtc cggatgacat tatgcggcct
     4861 gtcatcggta actatatctt ctgaataaac cagcgcgccg tcatccggat aattgctgtc
     4921 ctcgtaattt gaggcataga ctttcaggcg cgctccctga atttcattat gtatatcgat
     4981 gaagcggcac ctgatttcaa gctgctcctg atgcggtgca aattgttcat cgattccctc
     5041 tatcaccacc ccgggaaggc cgatctgcat ggcattatcg gagctttcgt actcagcgcc
     5101 cagggactcg atcaccgccc ggaatcggaa aattcggaat aggtctggtc ccagatgatc
     5161 tttcagtgcc tcttcatccg tttcaccgcc aagcggtgta tatgcaatcc gcaaacgatt
     5221 tgagctgatc tgcccgctta agctatccgg ctgaacatta tcgggatcgt cttcagcaaa
     5281 gacagtgata ctgacttcac tgttgtcgcc gacattttct gtttcgatga taatatgtat
     5341 ttcctgaccg ggtctgacat tctcggagtc gaaataggcg cgccttatgg ttgccggcag
     5401 agtctcgaca tgctcggtat cagttgatct gctgattcga tcgataccca attgtaacta
     5461 tccctgttta atctgtttta actggactgc tccagcgtcg aaccgtcatc cagccggact
     5521 ttaatattgc cgaccggaac ttcctcttcc agtatccggc cctgctcatc ggtcgaaccc
     5581 tcgcgagtgg aaccatcagg caatatcagc acataaccgg tattggccac cggattgcca
     5641 tccgaatcag tcagggtggc ctcgaataca tccaccaccg tcaggacatc gcttttggtc
     5701 tccagcgacc cggatttggc cttgaaaata aacctgggct tcgtgccctc atcctcttcc
     5761 tgaaattcat aggtccagac cgcctcggcc tgggaatttg catccacagt gccgtctatc
     5821 gatgttatct catcgcccga ttcttccttg aacagcttat aaatctcgaa tgttattgaa
     5881 tctccctcat catgtgccgg acattcaacc agcagcctga cctcatcccc gcagttgacc
     5941 tgatccacct cccagtgggc attggcaagc tgcttgaaaa taatctggct ctcgccctgg
     6001 ggcaggttat ccttgtgaat cttgccgtcg ctgccgactg atccggtgta acgcgtgccg
     6061 tctggcgcga tgataatata atccaatccc gagaccgcct cctcggccga atcctgcagc
     6121 gtaacatcca gatagtgctg gttctgttcc tgctcctctt cggcctgctc ctcgggcggg
     6181 gccggtggac ggcccaggga atttttcttg acctcaaatt ttgtggaata acccgaggcg
     6241 gaaaatatat gtgtggctcg agtaacataa taggaaccgg agaacttgtc acccagccct
     6301 tcaatatcga ttacagttcc agccttgagc tccggttccc ccggagcctt gccttcaccg
     6361 gtgataaaat tcatcgagag cttattgaaa tgagcgcggg ccaatgcatc cgcctcggcc
     6421 tggctgtgaa tcggcttgtt tactttgaaa ctcttggtcg atccatgagc ggcctcggca
     6481 atctgggtag cggtctgcgc tcctcccagc gtcgtgtgct cgtcaccgcc tccggcacgt
     6541 cccacaattt ccttcttgga tttcggatcc cagcccctga caaccacctc cgaaacctga
     6601 ttgaacgatg acagccgggg tgaaaaggag gccagcgatt cgccccattt tagagtcacc
     6661 ctccgggctt gatccgactg cggccttcgg aaatacagat cattaccctc gatatacatc
     6721 tcatacatta tcctctgcgc ccgctccagc agaaactcga gattggcctg atttctctgg
     6781 aagacatagg gatgaacctc ggtggtggca tccacatgcg cgctcagccc catctcgcgg
     6841 gctatctggt tggcaatatc cgaatccttc atctccacaa aggaacggct cttcttctca
     6901 cgcgacagcc ggaatgactt gtcgtaccca cgtatggtca aatatgccgc ccctcccgga
     6961 ggatactccg gttccaaaga aacaacttcg caatcggcaa ctgtgctcaa ctggctctcc
     7021 tgatagccca gcttcacctc aactgatttt ccaatagcca ggtcatcgga gttgaccagt
     7081 tccaggtcaa aatcgtagaa ccggatttca aacatatcca ggatttccag attactctcc
     7141 gctttaatga aaagtaccct ggattcgatt cgggtgtcca ggggctggcc gtctacaagt
     7201 atcttgaatg atggtgtcag gagctgattg ttctgctgct cctgatcctg agtttgtgac
     7261 cggggcatat attattcagc caggtatttt tgtttcgtga atttggcttc gatcgcttcc
     7321 tttatgaaag caactgcatc ctcaatgtca atataatttg tgctgaggat catgtcgtaa
     7381 tattcgggat cgttgatgtc gcgtttgaaa ttgcgcttga taaaatcctg ccgctccttg
     7441 tcgaaatctg caatctgctg ccgggccccc tcctgcgaca gattctcata tttcatcagg
     7501 ttcttctccc tgatcgcact ggaagccacc acgcgcagat ggaaaccggt ttgtatggtc
     7561 aatatgaaat tcgaaccccg gccgaccacc accactcccc ccaggcgcga cagggtaatg
     7621 atcgcccggt agaggtgcct gaaataatct gacgcatcgg aataccgccc tttgaaaata
     7681 ccttccagcc agagctcaac ctggttgcgg tatttctcat ccagcgattc tatcagacgc
     7741 cttcttattc ccgacgagtt gacaatatgt tcaattattt ccttgtgcat taactggtac
     7801 ccgagttcct cggccaggcg ctgggccagg tagcttcccc ggctcccgcg catacgggat
     7861 actgtgataa tcggcggcgg aagctggcgt tcgacctcgc cattctcacg cttctctttc
     7921 agaagctccc atttccgaat ttgacgatct acaatctgct ctattgatgg catggcacat
     7981 tctcctgctt ataaaac
//
LOCUS       DSEB01000236           10911 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000244, whole genome shotgun sequence.
ACCESSION   DSEB01000236 DSEB01000000
VERSION     DSEB01000236.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10911)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10911)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10911
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000244"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            3..623
                     /locus_tag="ENO07_04165"
     CDS             3..623
                     /locus_tag="ENO07_04165"
                     /inference="COORDINATES: protein motif:HMM:PF16653.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01194.1"
                     /translation="MKVIKNGKVVEVDAAGDLESFKFDKLGKNEILECAVTPGMPSFI
                     YTRPKLREFAEKTVRWPGHWQGVYTLKECGLLDVEPVVFNGSKIAPREFLLSLITPRL
                     RASEGETDVCVMYNTLTGKKGGKNVKIEYFMWDVADTKNNISSMARVTGFPVALALRL
                     ILNGEIKERGIVPPEDCIKGKIYNWFMKELKKRNIRILEVSNLKKK"
     gene            complement(725..3046)
                     /locus_tag="ENO07_04170"
     CDS             complement(725..3046)
                     /locus_tag="ENO07_04170"
                     /inference="COORDINATES: protein motif:HMM:PF00069.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HDS01195.1"
                     /translation="MTQSDKFGNSDPNQKNDRPSDDKTETAGHTDFGNRAKLPAKIGQ
                     YVIKRVIASGGMGTVYEALQENPRRPVAVKVIKDAFTNENAIQRLEYEAQLLARLRHP
                     GIAQIYETGSYNDGGNQIPFFAMEYIPNARSITVYAADKKLSIKDRLRLFLQVCDAVH
                     HGHQRGIVHRDLKPSNILVDSSGRIKIIDFGVARATDADMRQAASHTKVGQIVGSIQY
                     MSPEQFDADPHDIDTRSDIYALGLILYELLSEALPYECSSDRIFDFASEVREGKLTPL
                     RARNKSLKGEIEAIVHKALKRNREERYQSAYGLESDIKRYLAGDAISARTLGLSYQFR
                     VFARKNKAVMALLGSAFVLLLIGIIITTALFVRVDRERQKAEIATQKAARGQKFLTDL
                     LTSSFPSGFGDDYSVVDILDHASEKLYDAFPDDPELEWNLRSSIGHAYLNLDHYRQSE
                     EEFLRAYELIKSIYDVTHDKTLETLEQLSLVYSILGDERSKLEILRTYTNAIIERYGE
                     DSPQASSGRYSLAAILESNGLISEARQMAEKAWISFKKSSGPDGGSTLFAQIRYAWLL
                     MKSDDMEKAFQIAHDAFDRARRSYDITHSVYKNVQSCLAGLYIAEAKIDSARILYGDR
                     RIPEDFGVELSFQGDLDLNEKPFQLLVFFEAWCPFSKHAMTELGRVDIHYSEIGLDIY
                     GFTKVTKNLKDEDIYPYLSNLDIDFAVLKENGDCGKYFDVSGYPSIRLLCNGYLIWEK
                     QWFTSDFISSRMLNGIVAAQTCSPFVADGLAKN"
     gene            3559..4788
                     /locus_tag="ENO07_04175"
     CDS             3559..4788
                     /locus_tag="ENO07_04175"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00072.22,HMM:PF13189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDS01196.1"
                     /translation="MSVITIFSASYCHGEEIAKLLAEKLGYRFVSSEVLEMAADEYSI
                     SKDKLMASITGSSSLLGKILEDRDRNVAYVRVAAAKMASKDNLVYLGYAGLLLPKEIS
                     HILRVCLVADSAYRVKRAREIEDISEDKAEAIIKKDDDQRLNWTNYLYNVVPWDESLH
                     DIILPMHTLKVNDAVALIIENARKDVVKTTPESKKAIEDFILAAQVKLLMVEKKQNVD
                     VSAENGKIIITLKKYVMRLEHHKDELRKIAKKVPGVEEVEFKIGPKFRMPATYPPVEL
                     GVPKKTLLVDDEVEFVQTLSERLQSRQIKSSVAYNGEEALSVVEKDAPEVMVLDLKMP
                     GIDGIEVLRRTKRDHPETEIIILTGHGSERERQRALELGAFAYLEKPVDIDVLAETMK
                     KAYAKVNLSKQSDETTE"
     gene            4873..6603
                     /locus_tag="ENO07_04180"
     CDS             4873..6603
                     /locus_tag="ENO07_04180"
                     /inference="COORDINATES: protein
                     motif:HMM:PF02518.24,HMM:PF02743.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDS01197.1"
                     /translation="MKLSEEWHKKDLDYSSDEDDFFQEGRFRYRRLKRYSILLTALVA
                     ITPLIIMTIINSHQYQKALKADLIYPISRQTSNTKRSLESFIEERRAALDLISKEKNY
                     EELYDHDKLANTLRHLKESIDGFVDLSLIDSRGNQVAYVGPYKLEGKNYSDQDWFHEV
                     SIKGVYVSDVFLGYRDLPHFVIAVLHEKTEGDYYVLRATIDSDMLYRKIVSQILRPTS
                     DAFLINKAGILQTPSNDHGAILEKSPIPVPPYSPHSEVVEQKDSARNLYILGYAFIEN
                     SPFILIEVIQPEAFMQNWLTLRNNLIWFLGISIILILIVVIWGSSHMVDKIREADMHR
                     CKVLHQVEYTNKMASIGRLAAGVSHEINNPLAIINENAGMVNDVLSVSNDFPMREKVT
                     KSVNSILRNVERCSRITHRLLGFAKRMETKIETLDLRELITEVLGFLEKEALHRNIKI
                     NTNFPENLPGIRSDRGQLQQVFLNILNNAIDAISEGGKIDIDIELRKDNKIDVTIRDT
                     GRGISKPDLERIFEPFFSTKKEHGTGLGLSITYGIVQKLGGQIRATSELGEGTTFTVT
                     LPIEASFAEE"
     gene            6615..6977
                     /locus_tag="ENO07_04185"
     CDS             6615..6977
                     /locus_tag="ENO07_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDS01198.1"
                     /translation="MHILLVDDEEELVSTLADRLEFRGYHADAVLSGADAIKKIGQKS
                     YNVAVIDVKMPEINGIEVMKMMLKLQPDIKIILMTGHGSTEEGERGMAEGAFDYMVKP
                     INIDLLTDKIKTAVGIRE"
     gene            6985..7668
                     /locus_tag="ENO07_04190"
     CDS             6985..7668
                     /locus_tag="ENO07_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDS01199.1"
                     /translation="MSSTDQPDSRAQSLAFFGSITASVTHEIMNVIAIINELTGLLDD
                     MRYSADQGQVIESERLESLHERFSKQVSRGEKIIKRLNKFAHSADYDLIEFDLNETFR
                     NLIGLMQRLADMKRINLSFEPSPAELRCTASPFELQHLIFRCGRMLLNSGEEGATVSL
                     GVQKQADCYSINVTCSDIPDQGPERDELELVTELAAAQGWNLQSRKEDSKFLFILTMP
                     VRQTKDGDC"
     gene            7745..8176
                     /locus_tag="ENO07_04195"
     CDS             7745..8176
                     /locus_tag="ENO07_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDS01200.1"
                     /translation="MAETKVLLIDDEVEFTRTLSERLESRGLKVDSANSGEEALKKID
                     EVTYDAIFLDLAMPGMDGIETLKNLLAEKPDLQVILLTGHASIDKSVEAVKLGAKDFL
                     EKPANIDKLMAKIKEAETDRMLIVEKRSEDEIKKILKSKGW"
     gene            8399..10534
                     /locus_tag="ENO07_04200"
     CDS             8399..10534
                     /locus_tag="ENO07_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HDS01201.1"
                     /translation="MLQRIFPFLAWFKGYSLPFMRADFVSGLTVALVLIPQSMAYAQL
                     AGLPAYYGLYAAFLPPLVASLFGSSRQLATGPVAVVSLMTATALEPIATAGSEAYIAY
                     AILLALLVGIFQFLLGLFRLGLVVNFLSHPVVNGFTNAAAIIIATSQLSKLFGVNVDK
                     AEHHYMTVYNVVEAALAHTHWPTLVLAILAFAIMIGLKRMSPKIPYVLVAVIITTIIS
                     WAIGFEHNYNTAGENLLCERTKELIQDYNGALTDIKLRSQKRLLLRDELDSMKQEYSE
                     DSPHILNLQHQISLLNVEINELKEKVKHDRSLLRESFFKASENPDGSLVFCNTENLLP
                     QREYGHRDWQLKVGNGKLDMSSLTFVGGGAVVGKIPAGLPGFKKPQLDFKVMIDLFPM
                     AVIISLLGFMEAISIAKAMAARTGQRLDPNQELIGQGAANIVGCFGQSYAVSGSFSRS
                     AVNLQSGAVSGLSSVISSIFVMITLLLFTPLLYHLPQSVLAAIIMMAVIGLINVSGFI
                     HSWKAQKYDGVISIITFVCTLAFAPHLDKGILIGVGLSLILFLLRGMRPNIAMLSKHP
                     DGTYRNLERFGLAQCRHIAVIRYNGSLYFASVNYLERQVLDVVACMPELRHVLIVGNG
                     INELDASGEDMLSHLTDRLREAGYDLSISGLNDHVLDTMRRTYLYYKIGEDHLFRNAD
                     RAIAAIHASAHKDSSERKCPLLEVVYGNK"
BASE COUNT         2965 a         2487 c         2631 g         2828 t
ORIGIN      
        1 agctgaaagt tattaagaac ggcaaagttg tcgaggttga tgccgccggt gacctggaga
       61 gcttcaaatt cgataagctt ggcaagaatg agattcttga atgtgcagtt actcccggaa
      121 tgccgagctt catctatacc aggccgaaac tgcgggaatt tgcagagaaa acggtaaggt
      181 ggcccggcca ctggcagggc gtgtataccc tcaaggaatg tggcctgctc gatgtcgagc
      241 cggtagtttt caatggcagc aagatcgcac ccagagaatt cctgctttca ttgatcactc
      301 cccgtctcag agccagcgaa ggagaaaccg atgtctgtgt tatgtataac actttgaccg
      361 gtaaaaaagg cgggaagaat gtcaagattg aatactttat gtgggatgtg gccgacacca
      421 aaaacaacat ctcatccatg gctcgcgtga ccggctttcc ggtggcgctc gccttgcggc
      481 tcatcctcaa tggagaaatc aaagagcggg gtatcgtgcc gccggaagac tgtatcaagg
      541 ggaagatata caactggttc atgaaagaac tcaaaaaacg gaatatccgg atacttgagg
      601 tgtctaatct gaagaaaaag tgatgtcgat tctgattgaa gattggctgc cccggtgcaa
      661 gccggggctg ttgcttcacc caaaatcaga acatcttttc acatactttc tacattttca
      721 gtgatcaatt cttggccaga ccatcggcga caaacggtga gcaggtttgg gcggcaacaa
      781 ttccgttaag cattcgtgat gaaatgaaat cgctcgtgaa ccattgtttt tcccagatca
      841 ggtaaccgtt gcacaagagc ctgatggaag gataaccgga cacatcaaaa tattttccac
      901 aatccccatt ttctttcaaa acagcgaaat ctatatcaag attgctcagg tagggataga
      961 tgtcttcatc tttcagattt tttgtgacct ttgtgaagcc atatatatcc aggccgattt
     1021 cgctgtaatg gatgtccacc cgccctaact cggtcatagc atgcttagaa aatgggcacc
     1081 aggcctcaaa gaaaactaga agctgaaacg gtttttcatt taggtccaaa tccccttgaa
     1141 aagataattc aaccccaaaa tcttccggta tcctcctgtc accatacaaa atccgagcgg
     1201 agtctatttt tgcctccgcg atatataagc ctgccagaca gctttgtaca ttcttataaa
     1261 cgctatgagt aatatcatac gaacgtctgg cgcggtcgaa agcgtcatga gcaatctgga
     1321 aggctttttc catatcatcg gatttcatta gaagccaggc atatctaatt tgagcaaata
     1381 gtgtgctgcc gccatcagga ccggagcttt tcttgaaact aatccatgct ttttccgcca
     1441 tctgcctggc ttcagatatc agtccattgc tttcgagtat tgcggccaga ctatacctgc
     1501 cggaggaagc ctggggagaa tcctcgccat agcgttcaat aatcgcattt gtataggtcc
     1561 gcagtatctc taatttcgat ctctcatcac cgagtatgga gtagacaagt gaaagctgtt
     1621 caagtgtctc taaagtttta tcatgggtaa cgtcatatat gctttttatc aattcataag
     1681 cgcgcagaaa ctcctcttcg ctctgtctat aatgatccaa atttaaatag gcatgtccaa
     1741 ttgagctgcg caaattccac tccaattcag gatcgtcagg aaaagcatcg taaagtttct
     1801 cgctggcatg atccagaata tccacgacgg aataatcatc gccaaagcct gagggaaacg
     1861 aggaagttag gagatcagta agaaactttt gacctcgggc cgctttttgg gtggcaatct
     1921 cagccttctg cctttcccga tccactctga cgaatagagc tgtagtaata attataccaa
     1981 taagcagcag tacaaacgcg cttcctaaca gcgccattac cgctttgttt ttccgggcaa
     2041 aaaccctgaa ctgataagat aatccgagag ttcgtgcgga gatcgcatcc cccgcaagat
     2101 agcgctttat atctgattcc aggccataag cagattgata acgttcttcc ctgtttctct
     2161 taagagcctt gtgaacgata gcttcgattt cccctttcaa tgacttattc ctggccctca
     2221 atggcgtcaa ctttccttca cgcacctcgg atgcaaaatc gaatattcta tcagagctgc
     2281 attcataagg caacgcctcc gaaagtaatt cgtatagtat caggcccagc gcataaatat
     2341 cgcttcgagt atcaatatca tggggatcag catcaaactg ctcgggactc atgtactgaa
     2401 tggaacctac aatctgaccg acttttgtat gggatgcagc ctgtctcata tcggcgtcgg
     2461 tggcgcgcgc gaccccgaaa tcaataattt ttatgcggcc gctggaatcg accagaatat
     2521 tggatggctt gagatcgcga tggactatgc cgcgttgatg gccatgatga acggcgtcgc
     2581 acacctgcag aaataatctc aagcgatctt ttatgctgag tttcttgtcg gcggcataaa
     2641 ccgtgatgga tcgtgcattg ggaatatatt ccattgcgaa gaaaggaatt tgattgcccc
     2701 cgtcattata agagccggtt tcatagattt gcgcaatgcc gggatggcgc aggcgcgcca
     2761 gaagctgtgc ttcatattcc agtcgctgga tcgcattttc atttgtgaaa gcatctttga
     2821 tgactttgac ggctacaggt cgacgcggat tctcctgcag cgcctcatag actgttccca
     2881 tccccccgga agcgatgacc cgcttgataa catactgacc tatcttggca gggagcttag
     2941 ccctgtttcc gaaatcagta tgcccggctg tttctgtttt atcatcgctt gggcgatcat
     3001 ttttttggtt cgggtcggag tttccaaatt tatcggattg agtcatagag cacctgaaat
     3061 tacccgtttt cttgacaagg aataattatt aatgattata aaaataaaac gagataagtc
     3121 aagtcctatt tgtggccaag tatttaaatt ttatttaaca ggttccgaca catagggtta
     3181 tagaatatca cttaatgaac gaatcacatt aattcttacc ttttttctct acattacctt
     3241 tagcgggaca acacgcatca agtaattcaa ccgtactcga gctttatcca gtctttgaca
     3301 gctaaccttc ccccggcgcc tgctttactc tcccctgaag cggccgtttt gattgataca
     3361 ttgctttcgc ttcttgtgtc ggttggagca aagcaatttt cgagtaagca tcgcgcaaat
     3421 ttaggactca tgcctgttta catacagatt tcaggcccta tattaccaaa atgaacttgc
     3481 atttatttta aaagatgcat tatttgaaac attatatatt tttgacggtt ctgccgcggt
     3541 ttaaattcag gagatccaat gtcagtcatt acaatattca gcgcttccta ttgccacggt
     3601 gaggagattg ccaaactgct ggccgagaag ctgggctatc gctttgtatc atccgaggtg
     3661 ctggagatgg cggcggatga gtattcaatt tcaaaagaca aactgatggc ctctataacc
     3721 ggctcttcct ccctgctggg caaaattctc gaggacagag accgcaatgt tgcatatgtc
     3781 agggttgcag cggcaaagat ggcgagcaag gacaatctgg tatatctggg atatgccggg
     3841 ctcttgctgc ctaaggaaat ttctcatata ttacgcgttt gccttgtggc cgatagcgcg
     3901 tatcgagtca aacgagcccg cgagatagag gatatttccg aagacaaggc agaggcgatc
     3961 ataaaaaaag atgatgatca gcggctgaac tggaccaatt acctctataa tgtagtgccc
     4021 tgggatgaat ctctgcatga tataattttg cccatgcata ccttaaaggt aaatgatgct
     4081 gtggcactga taatcgagaa tgcccggaag gatgttgtca agaccacgcc cgaatcgaaa
     4141 aaggctatag aggatttcat tctggcggca caggttaagc tcctcatggt ggagaaaaag
     4201 caaaatgtcg atgtttcagc cgaaaacggt aaaataatta taacactgaa aaaatatgta
     4261 atgcgtcttg agcatcataa ggacgagcta cgtaagatag ccaaaaaggt cccgggtgtt
     4321 gaggaggtcg agttcaaaat cggccccaaa ttcagaatgc ctgcgaccta tccccctgtc
     4381 gagctgggtg tgcccaaaaa gaccttgctt gttgacgatg aggtggaatt tgtgcagacc
     4441 ctttcggaaa ggctccagag ccgccagata aagtcgtctg tagcctataa tggcgaagag
     4501 gcattatcgg tcgtggaaaa ggatgcaccc gaggtaatgg tactggacct gaagatgccc
     4561 gggattgacg ggatagaggt cttgcgccgc accaagcggg atcatcccga aacggaaatt
     4621 atcattttga ccgggcatgg ttcggaacgg gagaggcagc gcgccctgga actgggtgct
     4681 tttgcatatc tcgaaaaacc ggttgatata gatgttctgg cagagacaat gaaaaaggct
     4741 tacgcaaaag taaacctgtc aaagcaatct gatgaaacta cggaataatg cttttgattt
     4801 cgtagtattc taagattccc tgaggagcct tcgagtcaag gaagtgcttc taaattcgat
     4861 tttgagtgtg gtatgaaact ttccgaggaa tggcacaaaa aggacctgga ttactcatcc
     4921 gacgaagatg atttttttca agaagggcgc tttcgttatc gccgtttgaa aaggtattcg
     4981 attctgctga ccgcgctggt tgccatcacc cccctgataa tcatgacaat catcaactcc
     5041 catcaatatc aaaaagctct gaaagccgat ttgatctacc cgatttcccg gcagacctcc
     5101 aataccaagc gctcgctgga atcattcatc gaggagcgcc gggccgcact ggacctgatc
     5161 agcaaagaaa aaaactacga ggagctttat gatcatgata aattggccaa cactctcagg
     5221 catctgaagg aatcaatcga cggtttcgtc gatctcagcc tgatcgactc gagaggtaac
     5281 caggtggcct acgtggggcc atataaactg gagggcaaga attatagtga ccaggactgg
     5341 tttcatgagg tcagcataaa gggcgtttat gttagcgatg tcttcctggg ctaccgcgac
     5401 ctgccgcatt ttgtgatcgc agtccttcac gagaagacag aaggggatta ctatgttctg
     5461 cgagccacga tcgacagtga tatgctgtat cgcaagatcg tgtctcaaat tcttcgtccc
     5521 acaagcgacg cttttctgat caataaagcc ggaatcctgc agaccccctc aaatgaccat
     5581 ggagctatcc tggaaaaatc accgattccg gtacctccgt actctccgca tagcgaggtg
     5641 gtggagcaaa aggattcggc ccgtaattta tatattttgg gctatgcgtt catagaaaat
     5701 tcgcccttca tcctgataga ggtaatccag ccggaagcat tcatgcagaa ttggctgaca
     5761 ctgcgcaaca atcttatctg gtttttggga atcagcataa tcctgatcct gatcgtggtt
     5821 atctggggct cttctcatat ggttgacaaa atccgtgagg cggacatgca tcgttgtaaa
     5881 gttctgcacc aggttgaata caccaacaag atggcttcta tcggcaggct cgccgcggga
     5941 gtatcccatg agattaataa cccgctggca attataaatg aaaatgccgg tatggtaaat
     6001 gatgtactct cggtgtcaaa tgattttccg atgcgtgaga aagtcactaa aagcgtaaac
     6061 tccatactca gaaatgtgga gcgctgcagc cgaattaccc atcggcttct tggcttcgcc
     6121 aagcggatgg agacaaaaat tgaaacactg gatctacggg aattgatcac cgaggtgctt
     6181 ggatttttgg aaaaggaagc tctccatcgc aatataaaga tcaatacaaa ttttccagag
     6241 aatcttcccg gaattagaag tgacagggga caactccagc aggtctttct aaatatctta
     6301 aataatgcca tcgatgcaat ctcggagggg ggcaagatag atattgacat cgaattacga
     6361 aaagacaata aaattgatgt tacaataaga gatacaggac gcggaatctc aaagccggac
     6421 ctggagcgaa tcttcgaacc atttttcagc accaagaaag agcatggcac gggactgggt
     6481 ctgtcgatta catacgggat cgtgcagaaa ctgggcgggc agatccgcgc cacaagcgaa
     6541 ttgggtgaag ggacaacttt cactgtcaca cttccaatcg aggcatcttt tgccgaggag
     6601 taaatatggc tgatctgcac attttactgg tcgatgatga agaggaactg gtctccaccc
     6661 tcgccgatcg gcttgaattc cgaggctatc atgccgatgc ggttctgagc ggagccgatg
     6721 ctattaagaa aataggacag aaaagctaca atgtggcggt catagacgtt aaaatgccgg
     6781 agattaacgg tatcgaggtt atgaagatga tgctgaaatt gcagccggat attaaaatca
     6841 tactgatgac cgggcacggt tcgactgagg agggagaacg gggcatggcc gagggagcct
     6901 tcgactatat ggtaaaaccg atcaatatcg acctcctgac agacaagatc aaaaccgcag
     6961 ttgggattag agagtagcag tatcatgagc agcacggatc aaccggactc aagagcccaa
     7021 agtctggcat tttttggatc gataaccgcc agcgtcaccc atgaaataat gaatgtcatt
     7081 gcgatcatca atgaattgac cggtttgctg gatgatatga ggtattctgc ggatcaggga
     7141 caggtaatcg aaagcgagag gctcgaaagt ctgcatgagc gcttttccaa acaggtgagc
     7201 cgcggtgaaa agataattaa gaggctcaat aaatttgcgc attctgcgga ctatgacctg
     7261 atcgaattcg atttaaacga gacttttcgg aaccttatcg ggctcatgca aaggctggct
     7321 gatatgaaaa gaattaatct cagcttcgag ccttccccgg cggagctgcg ctgtaccgcc
     7381 agcccttttg agcttcagca tttgatattc agatgcggcc ggatgctttt aaactccggt
     7441 gaggaaggtg caaccgtcag tcttggagta caaaagcagg ctgactgtta tagtataaat
     7501 gtgacctgct cagatatacc ggatcaggga cctgagcggg atgaacttga gctggtcaca
     7561 gagctggcag cagctcaggg ctggaatctg caatccagaa aagaggacag taaattttta
     7621 tttatcttga ccatgccggt cagacaaact aaagacggag actgttaagc cgcagatttg
     7681 tatatgtaga attaacataa agagatgatt ttttgcaatt tgagctgtaa taaggaggcc
     7741 ccgggtggct gaaacaaaag tattactgat tgatgacgag gttgaattta ccaggaccct
     7801 atctgaacgt ctggagtcgc ggggtttgaa ggtggattca gccaacagcg gtgaggaggc
     7861 cctcaagaaa attgatgaag ttacttatga cgccatcttt cttgatctgg cgatgcccgg
     7921 tatggatggt atcgaaaccc tcaaaaacct gctggccgag aaacctgatc ttcaggtaat
     7981 tcttcttaca gggcacgctt ccattgacaa gagtgtcgag gcagtgaagc tgggggccaa
     8041 agatttcctg gaaaagcccg ccaacatcga taagctgatg gccaaaatca aagaggctga
     8101 aacggacaga atgttgattg tggaaaagcg cagtgaggat gagatcaaga aaattctgaa
     8161 atccaaggga tggtaaaagc aaaccgtgag acaatatttt ttcgaggatt aatctgtttg
     8221 cttttccttg atatgtgagg atgagattct gagttgataa ataaagatca tacgctaaaa
     8281 gtgcatttac gtgcccgaat cagtcgggct gcgaagtcag gagaaaattt aacaaccgga
     8341 ttcggcaatg gttaagcagc ctgctaataa tatttgcgac agccagggaa aatctccgtt
     8401 gctgcagcgt atcttcccct ttcttgcgtg gtttaaggga tattcattgc ccttcatgag
     8461 ggcggatttt gtatccggtc ttacggtggc cctggtgctt attccgcaat ccatggccta
     8521 tgcgcaattg gcggggctgc cggcatacta tggtctctat gcggcctttc tccccccgct
     8581 ggttgcatcc ctgttcggct ccagccgtca gcttgccact ggaccggtcg ctgttgtttc
     8641 gttgatgacg gctaccgccc tcgagcctat tgctaccgct ggaagtgaag cttacatagc
     8701 ttatgccata cttctggctc ttctggtcgg tatatttcaa tttttgctgg gactattcag
     8761 gctcggcctg gtggtcaatt ttctgtccca tcccgttgtc aatggcttta ccaatgccgc
     8821 cgccatcatc attgccacat cacaactctc caagctgttc ggagttaacg tggacaaggc
     8881 ggaacatcac tatatgaccg tttacaacgt ggtcgaggct gcgcttgctc atacccactg
     8941 gcccactctg gttctggcaa ttctggcttt cgccataatg atcggcctga aacgtatgag
     9001 ccccaaaata ccatatgtgc tggttgcggt tataatcaca actatcatct cctgggcaat
     9061 cggcttcgag cacaattaca atactgcggg agaaaacctg ctttgtgaga gaaccaaaga
     9121 gttgattcag gattataatg gtgcgttgac tgacattaaa ctccgatctc agaaaaggct
     9181 tttactgcgc gacgaactgg atagcatgaa acaggaatat agcgaggatt cacctcatat
     9241 tctaaacctg cagcaccaga tatcgcttct caatgtcgag attaacgaat tgaaagagaa
     9301 ggtcaaacat gaccgcagtc tgctcaggga gagctttttc aaagccagcg aaaatcccga
     9361 tggcagcctg gtgttctgta atacggaaaa tctgctgccg cagagggaat atggtcaccg
     9421 cgactggcag ctcaaagtcg ggaatggcaa actcgacatg tcgtccctga cattcgtggg
     9481 cggaggagct gtggtaggca aaattcccgc agggctgccc ggattcaaga agccccagct
     9541 cgattttaaa gtaatgatcg atctgttccc catggccgta atcatctctc tattgggatt
     9601 catggaggcg atatcgattg caaaggcgat ggctgccaga accggacaaa ggcttgatcc
     9661 caaccaggaa ttaatcggcc agggtgccgc caatattgtt ggatgctttg gtcaaagcta
     9721 tgcggtatca ggctctttct ctcgttcggc agtaaatcta cagtccggcg ccgtctcggg
     9781 cctgtcaagc gtaatttcca gtatttttgt gatgatcacc ctgctcttgt tcacgccgct
     9841 tctgtaccat ctcccccaat cggttctcgc ggcgatcatc atgatggcgg taatcggcct
     9901 tattaacgtc agcggtttta tacactcctg gaaggctcaa aaatatgacg gcgtcataag
     9961 catcataacc ttcgtttgca ctctggcctt cgcgccccat ctcgataaag ggattctgat
    10021 cggtgtggga ttgtccttaa ttttattcct gttgcgcggt atgcgtccca atatcgccat
    10081 gctatcgaaa catcctgatg gaacatatcg aaatcttgaa cgcttcgggt tggcacagtg
    10141 ccgccatatc gcggtgatcc gctacaatgg ctcgctgtac tttgccagcg tcaattatct
    10201 cgagcggcag gtactggatg tcgtcgcctg catgcccgaa ttaaggcatg tgttgatagt
    10261 cggaaatggt atcaacgaac ttgatgcttc gggcgaggac atgctctcgc acctgaccga
    10321 ccggttgcgc gaggcgggtt acgatttgtc cattagcggg ctcaacgatc atgtcctgga
    10381 taccatgcgc agaacctatt tgtattataa aatcggcgaa gaccatctgt tccgaaatgc
    10441 tgaccgggcc atcgcggcaa tacatgccag cgcacataaa gattccagcg aaagaaaatg
    10501 tcccctcctg gaggttgtat atggcaataa gtaaattggt tgggacatct catacatgtg
    10561 cttcgatcag attcgagata acaaaaaagc ggaaacctga tgtttccgct tttttttaga
    10621 attcgattat aatttacaat ttcaatctat ctgcgatagg caactccaaa acaaaagtct
    10681 ttaacataaa tatcgtcacc gtcatatccc tcggtgaatt ttattttcgt gatcggtatg
    10741 gtcgaaacta ttcccatgaa actcagaccg ccgctgggca tctggctata aggaatttcc
    10801 tcaatcttcc ggtcatggta aaatatttcc agtttttcgt ctgatgaaac gccgttatcc
    10861 ccgacataga tgccaacggc aaagacataa ctgtcattaa ggaagttgga g
//
LOCUS       DSEB01000237            1581 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1017054, whole genome shotgun sequence.
ACCESSION   DSEB01000237 DSEB01000000
VERSION     DSEB01000237.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1017054"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            142..>1581
                     /locus_tag="ENO07_04205"
     CDS             142..>1581
                     /locus_tag="ENO07_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01202.1"
                     /translation="MKDQIISQEHFLSGECAFTGRVDADGKITAVKEDSLSKKIGRAF
                     FSHVKYLALPEANIRAGQEIRDSLRQQYPKRQLKLIPLEWLSEALCNRNIIITEKVCL
                     LNRVSKGTRKYSRMAKVQVPLLGILVVILAAFTAIQIDPRLSPWFDYHISGIKVMGNQ
                     FQTINSEGKKIWDSEKFDRPFNKAQYENSAQLIQVFRPVDIDRNGRDELFFVPVFDKE
                     ESLLFFYDSRGRLEWKVTPFQKTAYPGDKAYPGVKENLHYHSVNVFPIIDNQGETFAL
                     TAAFTSLPAREQIILYDCDSNIVSGPYILTGSFIFETSIKLDINRDGQQDVLVYGTNN
                     RHEGCGIVVLDPKNLQGVSPPYDNKLFIMSGMPKGSQLRYIAFPETQLSEKEDVRTGI
                     LHLEFDEASGAIKVPILEGRGLIVDGSFYEPRDQMSPMLFYDLDSNFIPTGITFNDGT
                     KDHINDLLKRTNRAPIEDFEDLRDSLLSEV"
BASE COUNT          465 a          330 c          370 g          416 t
ORIGIN      
        1 cgaaaagtaa ttggcaggta tggtcacaag gaagcaagca aacgatttta caatgcacat
       61 ttctctatcg agaacagcaa gcagactttc accggcgatt caatcggcct ggctgcgggg
      121 ctggtgacat ttgtgcagct cttgaaggac cagataatat cgcaggagca ttttctttcc
      181 ggggagtgtg ccttcaccgg ccgcgttgat gcggatggaa agataactgc cgtgaaggag
      241 gatagtctgt cgaagaagat cggacgcgct ttcttctccc atgtcaaata tttggcattg
      301 cctgaggcga atatcagggc gggtcaagaa atacgtgatt ccctgagaca gcaatatccc
      361 aagcgccagc ttaaattgat tcctcttgaa tggctcagcg aggccctctg caaccgcaat
      421 attatcatca cggaaaaagt ctgcctcctg aatcgtgtca gcaaaggcac tcgaaagtat
      481 agccgtatgg ccaaggtaca ggtgccactt ttgggcatat tggtggtcat attggctgcc
      541 tttaccgcca ttcagattga cccaaggcta tcaccctggt tcgattatca catttcaggt
      601 attaaagtaa tgggaaatca attccagacc ataaattcag agggaaagaa gatctgggat
      661 tcggagaaat tcgatcgacc atttaataaa gctcaatatg aaaactccgc tcagttgatt
      721 caagtattta gaccagtgga tatcgaccgt aatggtcgag acgagttgtt ttttgtgcca
      781 gtatttgata aagaggaaag tctcttattc ttctatgaca gcagaggtag gctagaatgg
      841 aaagtaactc ctttccagaa gacagcatac cccggagata aagcctatcc cggtgtaaaa
      901 gagaacttgc attaccacag cgtaaatgtt tttccaatca tcgataatca aggagaaacc
      961 tttgcattga ctgcggcttt tacatctttg cctgctaggg aacagataat actctatgac
     1021 tgtgattcca atattgtgtc cgggccatat atcctgacag gtagcttcat ttttgagaca
     1081 agcattaagc tggacataaa tcgcgatgga cagcaagatg tactagttta cggaaccaac
     1141 aacagacatg agggttgtgg tatagtagtt ctcgacccga aaaatcttca aggtgttagt
     1201 ccgccctatg ataataaact tttcataatg tctggtatgc caaaggggag ccaactccgt
     1261 tatattgcgt ttcccgaaac ccagctgtct gaaaaagaag atgtccgtac tgggattctg
     1321 catcttgaat ttgatgaagc cagtggggcg atcaaagtgc caatacttga agggcgagga
     1381 ttaattgtcg atggcagttt ctacgaaccc cgagatcaaa tgagcccaat gctattctat
     1441 gatttggata gcaatttcat tccgaccggt atcactttca atgatgggac caaagatcat
     1501 ataaacgatc tcttgaaaag aactaataga gcaccgatcg aggattttga ggatttaaga
     1561 gattcactct taagcgaagt c
//
LOCUS       DSEB01000238           10995 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000242, whole genome shotgun sequence.
ACCESSION   DSEB01000238 DSEB01000000
VERSION     DSEB01000238.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10995)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10995)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10995
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000242"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1399)
                     /locus_tag="ENO07_04210"
     CDS             complement(<1..1399)
                     /locus_tag="ENO07_04210"
                     /inference="COORDINATES: protein motif:HMM:PF07969.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDS01203.1"
                     /translation="MNKERKTENLLLYNGRIYTQVPEAPFYQALGIAGGKIVWLGMDD
                     ELYQLPPDKFKIINLRGKTVLPSFFEAHMHFAFWAWSLGYIELSGCKSYQETLRAIKS
                     RSQELERGQWLVGQGWFKDGWENPVWPHKKDLDRLVPNHPAVMMSRDQHAIWVNSRAL
                     LKAGIRADTPDPRGGEIVRDESGEPTGILKDTAAQQVYRIMKPPTDVRGSLLLKTAEM
                     HAHSLGITGVASFDSIDGFRRVQSFHRKIGLKLRVSQFVPSDHLDELISLGIQSGFGD
                     RFLRIQGVKYFADGALGSQTALMFQPYKGTKSDYGLALYDDDELKAEVKRCARSGLNV
                     AIHAIGDRANRMALDAIIKAQGRSAGKFRNRIEHCQLLKKEDIERFGPHRIVGSVQPV
                     QMPLDIDMIKKYWGRRGRYSYALKSMLRSGARLAFGSDAPIEPPNPLLNIYLAVSRHP
                     RQTDKPFYPSERIRVE"
     gene            complement(1392..3263)
                     /gene="selB"
                     /locus_tag="ENO07_04215"
     CDS             complement(1392..3263)
                     /gene="selB"
                     /locus_tag="ENO07_04215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDS01204.1"
                     /translation="MIYTLATAGHIDHGKSTLVKALTGINPDRLPEEQQREMTIDLGF
                     AWFDLDDETEVGIVDVPGHERFIRNMISGVGGIDYVLFIVAADDGWMPQSQEHLEILD
                     FLGHKHGAVVITKVDLVREEWIELVEEDIRGKIEGSFLEGAPIFKFSSRGSRGLEEIL
                     DHLKGVLPGLPERYNPQKPRLYIDRVFSLTGIGTVVTGTMRDGSFKIGDEVRIISSGR
                     TARIKSIQTFKQQREVSFQGVRTALSLTGVSKEEITRGDCLAVPDQWQGTRNVALRVS
                     LSDLNHIPLSHNRKVTVLIGTTEIESSARVFKEDDFSKSDSGVCIFEFENRIMARLGD
                     SLILRYPTPDILVGGGVVTDVDLSRFIRRDKGMRKFYFERRLDSVRELIVSELRKYKT
                     REAGRLLEASNLHDDDINGAAKDLIESGEVEKYGNYLITAEYKISLFDKIIDYLKKFH
                     ADHPSRRGVSKSEITSKLKLEQAVIDFAVKQLQIEGRINISNNLLSMSEHETSLKPQQ
                     QKIRQDILDQLAVPDLMTPTRKEIEKNMHQIREVLNYLIQSGEVIELSDGILYRSEDF
                     DEIKHTVTDLIRKNGSVEVKDIKNAFGLTRKYTIPILEYLDRQGFTRREGDKRVLNE"
     gene            complement(3264..4634)
                     /locus_tag="ENO07_04220"
     CDS             complement(3264..4634)
                     /locus_tag="ENO07_04220"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:PRK04311.1-3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDS01205.1"
                     /translation="MQKSEIKTYKDIPQVEKILAWGELTEYVQKYSRPLVTIVVRKAV
                     EDLRTRIKDGKTSDRAKLLKSITRELDKIRFELTNKVINATGIVVHTNLGRAPLGEKT
                     FERLKDKISGYSSLEFDVLKGERGSRGSYLFELLSAMTGAEAGIAVNNNAAAVMLILN
                     TFGLNKKCVVSRGEQVQIGGGFRMPEVATRSGVNLIEVGTTNRTVIDDYRDAVDDETS
                     ILFKVHLSNFRLVGFTEETSLKELVALGKEKGLTVVHDLGSGAFVDTSEFGLGREPTI
                     QDSLMAGADLVCFSGDKLLGGPQAGMILGKKEKVAAVKKNPLYRTVRLDKMVIAILEE
                     LILSYMKSDYENIPAIALLSADNDELRKRAEKIRTALKDLKNEIEVVETEAFCGGGSL
                     PEESFPSVGIKVIDSKVTDLAKRLRMNNPPLIGRIKDDQLILDIRTIFPHEDKIVIEA
                     LQRALE"
     gene            complement(4992..5714)
                     /gene="bshB1"
                     /locus_tag="ENO07_04225"
     CDS             complement(4992..5714)
                     /gene="bshB1"
                     /locus_tag="ENO07_04225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HDS01206.1"
                     /translation="MSDDKLDVLAFAAHCDDIEITSGGLLIKLVDSGHKVGACDLTAG
                     EMGTKGSKEERLNESQCAAKVMGLTIRENLEFPDAQLEFKREYFMKMAEVIRKYQPHM
                     VILPHWEQRHPDHRICSQLGQDACYFSGLKKLDLPGEPHRPHKIIFSTYYRTMALPTF
                     YVDISQQFERKLEAVKCYKSQFDSTPESKQIFHPGTDIFDYIRTRDHNQGITIRTEYA
                     EAYIQKEPLQVDDPMTLKVRSV"
     gene            complement(5711..6832)
                     /gene="bshA"
                     /locus_tag="ENO07_04230"
     CDS             complement(5711..6832)
                     /gene="bshA"
                     /locus_tag="ENO07_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HDS01207.1"
                     /translation="MKIGIVCYPVIGGSGVVATELGIYLAQRGHQVHFFSYALPFRWD
                     PDLRNLFYHQVETSNYPLFKYPPYTLTLAAKIASIAVQENLDIVHAHYAIPHAICGYL
                     AREMIPEKNLKVITTLHGTDVTLVGSEKSFFDITRFSIVSSDASTAVSRHLKEEVCRT
                     FDLCNDIEVIYNFIDPAKYRPCDPCNLRKTYAPDGEKIVIHMSNFRSLKRIPDVVDTF
                     AGIHQKIKSRLILIGEGPDTVTAAQMAEKYGISKDVIFLGSRHDVASILSAGHLFLLP
                     SEHESFGLAAMEALCCGVPAIGTDSTGLPELIDDGKSGFLCKLGDTEDMAEKAVKLLS
                     DEDMWQSFSDHARKSVSEKFHYEKIVAQYEKMYEKVLSK"
     gene            complement(6834..8483)
                     /gene="bshC"
                     /locus_tag="ENO07_04235"
     CDS             complement(6834..8483)
                     /gene="bshC"
                     /locus_tag="ENO07_04235"
                     /inference="COORDINATES: protein motif:HMM:PF10079.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HDS01208.1"
                     /translation="MGCRTLSYNEIKGTSQLFKDFLFDFKKVAPYYCADFKDLDRFRG
                     KASKLAESFYARTELADILLDQNRQYGLGQKSLENVEKLKDEKSCVVFTGQQVGMLTG
                     PLYTIYKTLTSIKLANLLESEIGIPVIPIFWLAADDHDFEEVRHVYIIDKGGKPVRIY
                     YEPESDLGDVPMGFVKCDKKINTFLDEVLEALPKTEYSDDVERKVRESYREGTLLVDA
                     FGKMLGKFFENSGLVIVNPSDIRVKQLAAPIYAREIEEYESSNQIISIANTELDSLGY
                     HLQVHHREDYLNLFYYSGKRVRIGHNHEGYFIDGVDRKYTREELIDAVHQSPVYFSPN
                     VLLRPVTQDYLFPTLAYIAGPAEDAYFAQIKDLHGHFDVECPIVFPRISATILDRHAT
                     KTLDKYELKLPDLMDDRSLQAAIKEIVEEQVPDEVMEKFDEGREEITSRILEFEKYLE
                     NLDEGVRKTVHKTKGRVDYELKSLQEKIVKAYKKKNDNVVRAVQKCADFVFPEASLQE
                     RHLNVLTFINKYGPDFIDRISEHLKLDAYDHQVLCLEDIGY"
     gene            complement(8483..10993)
                     /locus_tag="ENO07_04240"
     CDS             complement(8483..10993)
                     /locus_tag="ENO07_04240"
                     /inference="COORDINATES: protein motif:HMM:PF00873.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDS01209.1"
                     /translation="MVSENFELPGGRLKKERSELLVRTMGKVNTVEDLKDFIIDTPKG
                     RFIKLSDIANISDGIKEQRSLARLGVREEDGFEIRRAISMDIITQSGANVVDVADAVK
                     EELEQLDQELPDDIILTVVQDRSTFTRDAVHDVVINLIYGGILAILTVFMFLANIRST
                     IITGIAIPVSIIATFTFMNALGFTLNFMTLLGLSLAVGLLIDDAIVVIENIYRHLSMG
                     KSGADAASDATSEIGLAVTATTFAIVVVFLPVAFMKGIAGRIFYSFGMTVAFSVLVSL
                     FVAFTLTPMLSSRFLKEEKSHKGSRNPIFIATNAWNRAFGWIGDIYQHMLRWSLDHRW
                     AIIVLATVFFVGALALYQVLGFEFMPRTDESQFYVNFKTAPESTIEKTEALVKKAESI
                     ISEFPEVITIYSVVGGSETMGQSQVNEGYIYAQMVPMDERDISAQEFIIDVRKALKDI
                     AGIKMAVALEPAEGGSAPINFAIRGESEERLEYYTEAVDDIFRSTPGVVDVDNTLTKV
                     KPEVQIEIDRKKAADLGVNLQELAMAVRILVEGEKVTRLRDEDEEYDILLRLKPDQRD
                     EIDDLESFMIPSRKEIAGMQDPAFPLSRLAKFVTRGAPPEINRYDRQRYYEVSANAAG
                     RFVGNVRSEIEVRADSIDLRPGYSIQQIGEGEWQKESFQYLLEALILAIIFIYLVLAS
                     QFNSFFDPVTIMTSLPLSLIGAFLGLLIFNSPVSIISFIGVIMLMGLVTKNAILLIDF
                     IKQRREAGMDRREAIMEAGPIRLRPIMMTALSLILGMTPLALALGPGAELRAPIARVV
                     IGGMISSTFLTLIVIPVVYTIIDDILKWPGKIVRRIKG"
BASE COUNT         2753 a         3019 c         2430 g         2793 t
ORIGIN      
        1 cttccaccct gatccgttca gacggataaa acggtttatc agtttgacgg gggtgacggg
       61 agacggccaa gtagatatta agcaacggat taggtggttc tatcggggcg tcggaaccga
      121 atgccagcct tgccccgctc ctgagcatgg acttcagggc ataggaatag cggccccttc
      181 tgccccaata tttcttgatc atatcgatat ccagcggcat ctgcaccggc tggaccgagc
      241 ccacgatccg gtggggccca aatctctcga tatcctcttt tttcaaaagc tgacagtgct
      301 cgatcctgtt cctgaacttc ccggctgatc ttccctgtgc cttgataatc gcatccaggg
      361 ccatacggtt ggcgcgatcg ccaatcgcat gaatagcgac attcagtcct gacctggcgc
      421 atcttttaac ctcagccttc agttcatcat catcataaag agccagacca taatcggatt
      481 ttgttccttt atagggttga aacatcaggg cggtctgcga gccgagagcg ccgtcggcaa
      541 aatacttgac cccctggatt ctcagaaatc tgtcaccgaa tccgctctga ataccaagtg
      601 atatcaattc atccagatga tccgaaggca caaactggct cacccgcagt ttgaggccta
      661 ttttcctgtg gaagctctga actctcctga atccatcaat agaatcgaag ctcgccacac
      721 ctgtgatacc cagagaatgc gcatgcatct ccgccgtttt taaaagcagc gaccctcgta
      781 catcggtggg cggtttcatt atcctgtaaa cctgctgggc ggcggtgtcc ttaaggatgc
      841 cggtgggctc accggactca tcacgaacaa tttcgccccc tctgggatcg ggggtatcgg
      901 cacggatacc agccttcaaa agagccctcg aatttaccca gatggcatgc tgatcgcggc
      961 tcatcatgac cgcaggatgg tttggaacca gcctgtccaa atccttttta tggggccaga
     1021 ccggattctc ccagccgtct ttgaaccatc cctgtccaac cagccactgg ccgcgctcga
     1081 gctcctgcga tctgctcttg atagcgcgca atgtctcctg atacgacttg catccggaca
     1141 gttcaatata tcccagcgac caggcccaga aggcgaagtg catgtgagcc tcgaagaatg
     1201 atggaagcac cgttttgccg cgaagattta tgatcttgaa tttgtccggt ggaagctggt
     1261 aaagttcatc atccatgccc agccagacga tcttaccgcc ggcaattccc agcgcctgat
     1321 aaaatggcgc ttccggcacc tgggtataaa tgcggccgtt gtaaagcagc agattctctg
     1381 ttttccgctc tttattcatt cagaacccgt ttatcgcctt cacgacgggt gaatccctgt
     1441 cgatcgagat attccagaat cgggatcgtg tatttacggg tcagtccgaa ggcatttttg
     1501 atatctttga cttccacaga accatttttc ctgataagat ccgttacagt atgcttgatt
     1561 tcatcaaaat cctcgctcct gtaaagaatc ccgtcagaaa gttcaataac ttcaccggac
     1621 tgtatcagat aattcaggac ttcccggatc tgatgcatgt tcttttctat ctcttttcgg
     1681 gtgggagtca tcaggtccgg taccgccagt tgatcaagga tatcctgccg gattttctgc
     1741 tgctgcggtt tcaggctggt ctcatgctcc gacatggaaa ggaggttatt ggagatattt
     1801 atcctgccct caatttgaag ctgcttgact gcaaaatcga ttacggcttg ctccagtttg
     1861 agcttggagg tgatttcact ttttgaaaca cctctgcggc tgggatgatc cgcatgaaat
     1921 tttttcaggt aatcgattat tttatcaaaa agacttattt tgtattcagc ggttataaga
     1981 taatttccat acttctcgac ctcgccgctc tcgatcaaat ccttcgcggc cccatttatg
     2041 tcatcatcat gtagattgga tgcttcaaga agtctgccgg cctcccgggt tttgtatttc
     2101 cgcagctctg atactatcaa ctcccggaca gaatccagcc tgcgttcaaa atagaacttt
     2161 ctcatacctt tatcacggcg aataaaacgc gacaggtcaa catccgtaac cacgcctccg
     2221 cctacaagga tatcaggagt cgggtagcgc aatattaagc tatcgcctaa acgcgccata
     2281 atcctgttct cgaattcgaa tatgcaaacc ccgctgtcgc ttttggagaa atcgtcctcc
     2341 ttaaataccc gggcactcga ttcaatttcc gtggtgccga tgagtactgt cactttgcga
     2401 ttgtgcgaaa gcgggatatg atttagatcg gaaagactta ccctgagagc gacattcctt
     2461 gtgccctgcc actgatccgg aactgccaag cagtcccccc gggtaatctc ttctttggac
     2521 acccccgtca atgacagcgc cgtcctgacg ccctggaaag atacttccct ctgctgcttg
     2581 aaagtctgga tagatttgat gcgcgctgtc ctccccgatg atataatccg cacctcatcg
     2641 ccaattttaa aactgccgtc acgcatggtg ccggtcacaa cagtgccgat acctgtgaga
     2701 gaaaacactc gatctatata caatctcggc ttctgcgggt tataacgttc gggaagaccg
     2761 ggaagcactc ccttgagatg atccagtatt tcctccaaac cgcgggaacc tctggaggaa
     2821 aatttgaaga tcggggcgcc ttccaaaaaa ctcccctcta ttttacctct gatatcttcc
     2881 tctacaagct cgatccactc ctccctgacc agatcgacct tggtgataac cactgctcca
     2941 tgcttgtggc cgagaaaatc aagtatctcc agatgctcct gggactgcgg catccagccg
     3001 tcatccgcgg ccacaatgaa aaggacataa tcgataccgc ccaccccgga gatcatattg
     3061 cggataaagc gctcatgtcc gggcacatcg acaatgccga cctcggtctc atcatcaaga
     3121 tcaaaccagg caaagcccag atcgatagtc atctccctct gctgctcctc gggcagacga
     3181 tccgggttga ttccagtcaa agccttgacc agagtggatt tgccatgatc gatatgtccc
     3241 gccgtggcca gagtatagat catctattcc agcgcccttt gcaaagcttc aatcacgatt
     3301 ttgtcctcat gcggaaatat tgtgcgaatg tccaggataa gctgatcatc cttgattctt
     3361 ccaatcagcg gcggattatt catgcgcaga cgttttgcca gatctgttac ctttgaatct
     3421 atgactttga ttccaacaga tggaaacgat tcctcgggaa gtgatccgcc tccgcaaaac
     3481 gcctcggtct cgactacttc gatctcattc ttcaaatctt tcaaggccgt tctaattttc
     3541 tccgcccgct tcctgagctc atcgttatca gccgacaaaa gcgcaatcgc cgggatattt
     3601 tcgtaatctg acttcatgta gctcagtatt aattcttcca ggatcgcgat caccatttta
     3661 tccaggcgga ccgtgcgata aagcggattc ttcttaaccg ccgcaacctt ctccttcttg
     3721 ccaaggatca taccagcctg ggggcctccc aggagtttat ccccggagaa gcagaccaga
     3781 tcggcaccgg ccatgagcga atcctgaatt gtaggctcac ggcccagtcc gaactctgat
     3841 gtatccacaa acgctcccga tccgaggtca tgcaccacag tcaggccctt ttctttgccc
     3901 agcgcaacca gttctttgag cgaggtctcc tcggtgaaac cgaccagcct gaaattggag
     3961 agatgcacct tgaaaagtat gctcgtttca tcatcaacag catcgcggta atcatcgatg
     4021 acggtacggt ttgtggtccc cacctcgatc aaattgaccc cgcttctggt ggccacctca
     4081 ggcatccgga atccgccccc gatctgtacc tgctcaccgc gcgatactac gcactttttg
     4141 ttcaagccaa atgtattcag gatcagcata acagctgcgg cattattgtt gaccgcaatt
     4201 ccagcttcgg ctccggtcat agcggacaaa agctcaaaca gatacgatcc ccggctcccc
     4261 cgttcccctt tcaggacatc aaactcgaga gatgagtaac ctgaaatttt gtcctttaag
     4321 cgctcgaatg ttttctcacc caggggagcc cgtcccagat ttgtatgtac cacaatcccc
     4381 gtagcattga tgaccttatt ggtcagttca aagcggattt tatcaagctc tcttgtgatc
     4441 gatttgagga gttttgctct atcggaggtc ttaccatcct taatccgggt gcgaagatct
     4501 tcaactgcct tgcggacaac gatagtgacc agcggcctgg agtacttctg gacatattcc
     4561 gtcaattccc cccaggccag gattttctcc acctggggaa tatctttgta tgttttgatt
     4621 tcggatttct gcataacctg caaaatatat taagcttacc cgcgacacac aagcattaac
     4681 tggcaaaaat aaaagcggaa ggggaaggaa tcgaaccctc cgctgccttt tggggcagcc
     4741 aacgggtttg aagcccgcgg gggccaccag accccatcct cttccgaatg ctgaatatat
     4801 ccattatcgc cccaaagtca aacttattga atacaatcgc cccgacatag ttccagcgaa
     4861 agcctgaaat tacagtgcca gtttaatctc aaacgattct ctcacataaa tagggtatga
     4921 tggcaacttt gaaggagaga acatgaaatc gcgtcaataa aattcataac tgaagagcgg
     4981 taaaacagat tctataccga cctgaccttc aaggtcatcg gatcatcaac ctgcagcggc
     5041 tctttctgga tgtaagcctc cgcgtattcg gtgcggatgg ttataccctg gttatggtct
     5101 ctggtcctga tataatcgaa aatatcggtg ccgggatgaa atatctgctt cgattcagga
     5161 gtgctgtcaa actgcgattt atagcatttt acagcctcca gcttacgttc aaactgctgc
     5221 gaaatatcga catagaatgt cggtagggcc atcgtacggt aataagttga aaaaataatc
     5281 ttatgtggac gatgcggctc gcccggcaga tcgagttttt tcaaaccgga aaaataacag
     5341 gcatcctggc ccagctgcga acagattcta tgatcgggat gacgctgttc ccagtgtgga
     5401 agtatcacca tgtgcggttg atacttcctg atcacctcgg ccatcttcat aaaatattcc
     5461 cgcttgaact cgagctgcgc atcaggaaat tccagattct ctcggattgt cagccccatt
     5521 accttagccg cacattgaga ctcattcaga cgctcctctt ttgagccctt cgtgcccatc
     5581 tcgccggcgg tcaaatcgca tgcgcccacc ttatggccgc tatcgaccag ctttatcagc
     5641 agcccccccg aagttatttc gatatcgtcg caatgcgccg caaatgccag gacatcaagc
     5701 ttgtcatcac tcatttactc aacaccttct catacatctt ctcatactga gcaacaattt
     5761 tttcgtaatg aaatttttcg gagaccgact tgcgcgcatg atccgaaaaa gactgccaca
     5821 tatcctcatc cgacagaagc ttcacggcct tctcggccat atcttctgta tcacccagct
     5881 tacacaaaaa gcccgacttg ccatcatcga tcaactcggg caaaccggtg gaatctgtgc
     5941 caatcgccgg caccccgcaa cacagggctt ccattgccgc caggccgaat gattcatgct
     6001 ccgacggcag caggaacagg tgtccggcag aaagaatgct agcgacatca tggcggcttc
     6061 caagaaagat cacatccttg gagataccgt atttctccgc catctgtgcc gcggtaacgg
     6121 tatccggacc ctcgccgatc aggatcagcc tgctttttat cttctgatgg attcctgcga
     6181 atgtatcgac aacgtccggg atccttttaa gcgacctgaa attggacata tgtattacaa
     6241 tcttttcacc atcgggggca taggtcttgc gcagattaca tggatcgcag ggcctgtatt
     6301 ttgcaggatc gatgaaatta tatatcactt ctatgtcatt gcatagatca aaagtcctgc
     6361 aaacctcttc cttgagatgg cgcgatactg cggtggaagc atccgagctt acaatcgaaa
     6421 atctggtaat atcaaaaaat gatttttcag aaccaaccag agtcacatca gtgccatgaa
     6481 gagtggtgat gaccttcagg ttcttctccg ggatcatctc cctggccagg tatccgcaga
     6541 tagcgtgagg tatggcataa tgggcatgca ctatatccag attctcttgg accgcaatac
     6601 tggcgatctt ggccgccagt gtcaatgtgt aggggggata cttgaagaga ggataattgg
     6661 aggtctccac ctgatgataa aacagattcc gcagatcggg atcccacctg aacggcagag
     6721 catacgagaa gaaatgtacc tgatgccccc gttgtgccag gtatataccc agctctgtag
     6781 caaccacccc ggagccgccg ataaccggat aacagacaat accgattttc acatcaatac
     6841 cctatatctt ccaggcagag cacctgatga tcgtaggcgt ccagcttgag atgttcagaa
     6901 atgcggtcta taaaatcggg tccgtattta tttatgaatg tcaggacatt caggtgacgc
     6961 tcctgcaggg acgcctccgg aaagacaaaa tccgcgcact tctgtacggc acgaactaca
     7021 ttgtcatttt tcttcttata agcctttacg attttttcct gcagcgattt caactcgtag
     7081 tcaactctgc ccttggtctt gtgcacagtc tttctgacac cctcgtccag attctcgagg
     7141 tatttctcga attcaagaat cctggaagta atctcttccc ggccttcatc aaatttttcc
     7201 attacctcat cgggcacctg ctcttcaaca atctctttta tggccgcctg aagtgacctg
     7261 tcatccatca agtcaggcag cttcagctcg tatttgtcaa gggtcttggt ggcatgccta
     7321 tccagtatgg tggcggagat gcggggaaat acgattggac attccacgtc gaaatgaccg
     7381 tgcagatcct taatctgggc gaagtacgca tcttcggccg gtccggcgat ataggccagg
     7441 gtcgggaata gataatcctg ggtaaccgga cgcagaagaa cattgggcga gaaatatacc
     7501 ggcgactgat gtacagcatc gatcagctct tccctggtat atttcctgtc gacgccgtca
     7561 atgaagtaac cctcatgatt atggcctata cggacacgtt ttccgctata ataaaacagg
     7621 ttcaaatagt cttcccggtg gtgtacctgc aggtgatacc ccagactgtc aagttcggta
     7681 ttggcgatgg agattatctg atttgatgat tcatattcct cgatctcccg ggcatatatg
     7741 ggcgcagcca gctgcttgac gcgaatatct gacgggttca ctatcaccag cccggagttc
     7801 tcaaagaact ttcccagcat cttaccaaag gcatccacca agagcgtgcc ttcccggtac
     7861 gattcccgaa ccttcctctc aacatcatcg ctatactcag ttttcggaag ggcttccagg
     7921 acttcatcca gaaatgtgtt tatcttctta tcacacttaa caaatcccat cggcacatcc
     7981 ccgagatctg attccggctc ataataaatc ctaaccggct taccgccctt atcgataata
     8041 tacacatggc gcacttcctc gaaatcatga tcgtcggcgg ccagccagaa gataggtata
     8101 accggaatcc caatttctga ttccagaaga ttggccagtt taatcgatgt cagggtctta
     8161 taaatggtat agaggggccc ggtcagcatt ccaacctgct ggccggtgaa aaccacgcat
     8221 gatttttcat ctttcagctt ttcaacgttc tccagagact tctggcccag gccatattgt
     8281 ctattttgat ccaggagaat atccgccagt tccgtgcggg catagaagga ttctgccagc
     8341 ttcgaagcct tgccccggaa tctgtccagg tccttgaaat cggcgcagta atagggcgcc
     8401 acctttttga aatcgaaaag gaaatctttg aataactggc ttgtcccttt tatctcatta
     8461 tagctaagcg tcctgcaacc catcaacctt ttatccttct gacaatcttt cccggccact
     8521 taagtatatc atcgattatt gtataaacaa caggtattac aataagtgtc aggaaagttg
     8581 aggaaatcat gccgcctata acgacacgtg cgattggtgc acgcagctcg gcgcccgggc
     8641 cgagtgcaag ggccagcggt gtcatgccca gaataagcga gagggccgtc atcataatcg
     8701 gcctgagcct gatcggcccg gcctccataa tcgcttccct tcggtccata ccggcctcac
     8761 gccgctgttt gataaaatct ataagcagaa tggcattctt ggtaacaagc cccattaaca
     8821 taataacccc aatgaacgaa ataatcgaaa ccggcgaatt gaaaatcaga agccccaaaa
     8881 atgcgccgat cagagacagg ggcagcgaag tcatgatcgt aaccggatca aaaaaactgt
     8941 taaactgtga ggccaggacg agatagatga agataattgc caggattaaa gcttccagaa
     9001 gatactggaa cgattccttc tgccactcgc cttcaccaat ctgttgaata gaatacccgg
     9061 gcctgagatc gatcgaatca gcacggactt ctatttccga gcgcacattg ccgacaaaac
     9121 gtccggcagc attggcgctg acctcataat acctctgccg gtcatacctg tttatctcag
     9181 gcggagcccc ccgggtaaca aacttcgcca gtctggaaag gggaaaggcc ggatcctgca
     9241 ttcccgcaat ttccttgcgc gagggaatca tgaaactttc cagatcatca atctcatccc
     9301 gctggtcagg cttcagcctg agaagaatat catactcctc atcctcgtcc cgcagacggg
     9361 taaccttttc accctcaacc agaatcctga ccgccattgc cagctcctga aggttcactc
     9421 ccagatctgc ggccttcttg cggtctatct ctatctgcac ctccggcttg accttggtaa
     9481 gggtattatc gacatccacc acaccgggtg tggacctgaa aatatcgtcc actgcttcgg
     9541 tataatattc aagccgctct tccgattctc cgcgtattgc aaaattaatc ggcgccgaac
     9601 ctccctccgc aggttccaga gccaccgcca tttttatccc cgcgatgtcc tttaatgctt
     9661 tcctgacatc tataataaat tcctgggcag aaatatcccg ttcatccatg ggaaccatct
     9721 gggcataaat atagccttca ttcacctggc tctgccccat tgtctcggag cctccaacca
     9781 ccgaataaat cgtaattacc tctggaaatt cggagatgat actttcggct tttttgacca
     9841 gcgcctcggt tttctcgatt gtactttcag gcgccgtctt gaagttcaca taaaactggc
     9901 tttcgtcggt ccggggcatg aattcaaacc ccaaaacctg gtaaagagcc agggcaccaa
     9961 caaagaacac ggtggccagc acgataattg cccatctgtg atccagagac caccgcagca
    10021 tatgctggta gatatccccg atccagccga aggcacggtt ccaggcgttg gtggcaataa
    10081 aaatcgggtt cctgctgccc ttatgtgatt tttcttcctt cagaaatctt gaagacagca
    10141 tcggggtcag agtaaacgcc acaaaaagtg agaccagtac cgaaaatgcc acggtcatac
    10201 cgaatgaata gaaaatccgc ccggcaatac ctttcatgaa ggccaccggc aaaaacacca
    10261 ccacaatcgc aaatgtggtc gccgttaccg caagaccgat ctccgacgtg gcgtctgacg
    10321 ctgcatctgc ccccgatttc cccatcgaca ggtgccgata aatattttca ataaccacaa
    10381 tcgcatcatc gatcaaaagt cccacagcca gcgacagtcc cagaagcgtc ataaagttca
    10441 gagtgaagcc aagcgcgttc atgaatgtga aggttgcaat aatcgaaacc ggaatcgcaa
    10501 ttcccgtgat aatggtcgaa cggatattgg ccaggaacat gaacaccgtc aggatcgcca
    10561 gaattccgcc gtaaataaga ttgataacca catcatgaac ggcgtcgcgg gtaaaggttg
    10621 atcgatcttg cactacggta aggatgatgt catcgggaag ctcctggtca agctgttcca
    10681 gttcttcctt aacggcatcc gcgacatcca ccacattggc gccggactgt gtaataatat
    10741 ccatcgaaat cgcccgcctg atctcgaaac catcttcttc ccggacacca agtcttgcta
    10801 atgagcgctg ttcctttatt ccgtctgaaa tattggcaat atcagagagc ttgataaagc
    10861 gtcccttggg ggtgtcaatg ataaaatctt tcaggtcctc cacagtgttg accttaccca
    10921 tggtcctgac cagaagttcg ctgcgttcct tctttaagcg tccccccgga agctcgaaat
    10981 tctccgagac tataa
//
LOCUS       DSEB01000239           11039 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000240, whole genome shotgun sequence.
ACCESSION   DSEB01000239 DSEB01000000
VERSION     DSEB01000239.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11039)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11039)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11039
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000240"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..465
                     /locus_tag="ENO07_04245"
     CDS             <1..465
                     /locus_tag="ENO07_04245"
                     /inference="COORDINATES: protein motif:HMM:PF01048.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01210.1"
                     /translation="THDYMYIGPDSMVVMNPVVYSPESDSMIQMRIFEVDDSLFAKTS
                     GLDEVVLDSIGGRRPRIIIGGTGVSGNQFIDNKDKRLWLNEKFDALIVDMESSAIAQT
                     CYVSEVPFIIFRSASDLAGGAETETAGEQLERFLEVAADNSSSVVIKFLEAL"
     gene            complement(816..1298)
                     /locus_tag="ENO07_04250"
     CDS             complement(816..1298)
                     /locus_tag="ENO07_04250"
                     /inference="COORDINATES: protein motif:HMM:PF13577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HDS01211.1"
                     /translation="MLLRYFAMLLAISLLLPGCENEDEQAAVDTAAEKEAVGDVLVAY
                     ISSVENENMADYAKNIGHDPEMVNFGAFGEPIRGRDDLEIVMTNQNEMLDNIQIAEND
                     PRIHISPSGDLAWATSLWQFHATAGADTMNLPVRCTWVLQRQDGQWKIVHFHKSLPAG
                     "
     gene            complement(1695..2225)
                     /locus_tag="ENO07_04255"
     CDS             complement(1695..2225)
                     /locus_tag="ENO07_04255"
                     /inference="COORDINATES: protein motif:HMM:PF01885.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HDS01212.1"
                     /translation="MTKKQSKFLAFLLRHGADEFNLRLDNHGWAQVHDVVGIMQRKNP
                     GLTVDDLKYIVETDSKERYQIENDRIRARYGHSIKVESREIPSKPPEVLYHGTARRNL
                     GLIFSKGLRPMRRQYVHLSTNIEDARNVGRRHSQNVAVLKIRAEDAHKSGITFFRESN
                     TYLTKEIPVEFIEMME"
     gene            complement(2376..3284)
                     /gene="murQ"
                     /locus_tag="ENO07_04260"
     CDS             complement(2376..3284)
                     /gene="murQ"
                     /locus_tag="ENO07_04260"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007331380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDS01213.1"
                     /translation="MSKKVFNEIKDLATESVNKWTRSIDILSTRKVLELINVEDHQVA
                     PAVRKVIPQVEKAVELVVKSFRSGGHLYYIGAGTSGRLGVLDASECPPTFGVKPSLVQ
                     GIIAGGRRTLVRSKEGIEDDAEAGRRDIEKKDVSSKDTVIAIATSNRTPYTLAGLKQA
                     KKMRASTVFLCCNRKVRLLFKPDVIINPITGPEAIAGSTRMKAGTATKLILNMITTTA
                     MVKIGKTYGNLMVDLQATSEKLVQRSIKTLITVCDLNYSQAKKLLERAEGHVKSAIVM
                     HFKRTDLKTAKNMLQLADGRLSRILK"
     gene            complement(3284..4060)
                     /locus_tag="ENO07_04265"
     CDS             complement(3284..4060)
                     /locus_tag="ENO07_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01214.1"
                     /translation="MLRKVSISIFLIGALVLAGCEGTVDGELDSEFAVNGLLLHNPND
                     NTSFAYAFILEDGEPIPALTSFLVTSNEDSTQLVPIGGGAYLTNEGMLNIAPDSSYVF
                     WAGEDLGDFYFSKTLDIADTFSVVVTRPATRIYSGGTVSIEWDPPSAEFGYFVTIEPP
                     SDAASPFWEFANSTNEFVIGAEAFTTSTGQRVTGIYKIYVVAYSETFYSDALIENDDR
                     VFFPYPDTGFANNIDLVDVKGRLGSAIISFYDSVTVIAQP"
     gene            4215..4364
                     /locus_tag="ENO07_04270"
     CDS             4215..4364
                     /locus_tag="ENO07_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012821725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDS01215.1"
                     /translation="MKRTYQPSRRKRLNDHGFRKRMSTKNGRKVLKRRRSKGRKRLTP
                     QITKK"
     gene            4291..4704
                     /gene="rnpA"
                     /locus_tag="ENO07_04275"
     CDS             4291..4704
                     /gene="rnpA"
                     /locus_tag="ENO07_04275"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:PF00825.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDS01216.1"
                     /translation="MGGRSSKDAGQKGASASRRRSPRNKGEYSFGREYSLKEDLAIKR
                     VFRSGRKFEGRCLVIYHYGAGSLPLKVALKVRRNIGSSPRRNRIKRIIREIIRTNKNK
                     FKHGGHLVISVIVDPGDETLFEAIEKDLFDFAQAR"
     gene            4709..4918
                     /gene="yidD"
                     /locus_tag="ENO07_04280"
     CDS             4709..4918
                     /gene="yidD"
                     /locus_tag="ENO07_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001295520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDS01217.1"
                     /translation="MAYPLILIIRIYQVAISPLFPTQCRFHPSCSNYAIEALNKHGLK
                     GIFMSIKRILKCHPFSKKSGYDPVR"
     gene            4923..6617
                     /locus_tag="ENO07_04285"
     CDS             4923..6617
                     /locus_tag="ENO07_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDS01218.1"
                     /translation="MDRKTVLALIIIGIIFVGWIYLTQPEPRPQPVETPVETDTLGVR
                     EATGDEAQTFEEQVDTAVAEGFEPIGGDSLYAAFDTLRVDTVQVETDKYIALFSSRGA
                     GLVSFKFKNYNYNNGDTTMVEMVPPWAEAALKFQFPDARDSFYFNRIVFMPEKNNIRL
                     SGGNKDKLTFTANLANQGSIMVEYEFYADRYDFSVRLDFQNASAFDLGRYYYFGWEPG
                     LESTEKNRQDDFNSFKAYVMWDTGLEGYSKFQNGQMSQEIEGNLQWVATKTKYFFVGI
                     APDRAPQGVRIQGREISVNEKAGQPGMKKIGVQAEMEIHQRKSDLFDKYLIYVGPIDY
                     KVLRSYNRGFEETVDLGWPIIKTISRFILWLMEVLYGIFHNYGVVIIIFSILMKVVFY
                     PLTSRSLRSMKKMQELQPKLKALQEKYKKEPQKMQAEIMKLWKENKVNPMSGCLLMLP
                     QLPIFFALFTVFRNTILLRGSEFMLWMKDLSQPDQTMILPLIMAGTMFLQQKMTMQDP
                     KQKMLVYILPIVFFFLFKGFPTGLVLYWTMFNVLSVLETLLIRKPQQQKELALKTE"
     gene            6614..8005
                     /gene="mnmE"
                     /locus_tag="ENO07_04290"
     CDS             6614..8005
                     /gene="mnmE"
                     /locus_tag="ENO07_04290"
                     /inference="COORDINATES: protein motif:HMM:PRK05291.1-3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDS01219.1"
                     /translation="MISYSEDTIAAVSTPPGEGGIAVVRISGKSSKQIADRIFRGRIT
                     PSEAGSHTVHFGKIVDPDSEELVDEVLLTVMWGPNSYTAEDVIEISSHGGQLVAKKIL
                     DLCLKTGARHALPGEFTQRAFLNGTIDLAQAEAVADMISARTDMSLRSAARQLGGNVS
                     QTVASLGNKIRNLLALLEAYTDFPEEEMGHADIQKLTDDLNIIKDEINRLVLSYEEGK
                     VLKSGLKLPIVGRPNVGKSSLFNRLLDESRTIVTEIPGTTRDTVSEFINIAGIPVELI
                     DTAGIRTTGDVVEAEGIKRARKEIENADLVLALVDMTDPEILDDIRSLTEALGSVNYI
                     LLANKADLIDETMLEARKISLAEYNPLPVSAETGIGLEQLRDLIIKSAGIVKKSSLKD
                     ETIITNVRHRDALSKGLGFLDKVEQGLEEKASYEFLAFDLKNAVNALEEIIGKTTPED
                     ILNKIFSQFCIGK"
     gene            8122..10005
                     /gene="mnmG"
                     /locus_tag="ENO07_04295"
     CDS             8122..10005
                     /gene="mnmG"
                     /locus_tag="ENO07_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDS01220.1"
                     /translation="MTNLHYEIVVAGGGHAGAEAALAASRMGCSTLLVSLRKDTIGQM
                     SCNPAIGGQAKGHLVKEIDALGGEMGLAIDRTGIQYRRLNLSRGPAVHSSRAQADRKL
                     YREYITGAVLNQGNLDFLEDAVVDLEVNGSHVSAAITEGGRKINCRCLIITAGTFLNG
                     LIHIGRKKIPAGRMGEKPSLGLSEKLIALGFKSGRLKTGTPPRLDGKTIDYSRTESQY
                     GDPDFPPFSFRSNSINGNKAICHITFTNQDTHRIILDNLDSSAMYSGQIKGIGPRYCP
                     SIEDKVVRFRDKDRHQIFLEPEGLDTDLIYPNGFPTSLDEEVQKKAIKTVIGLEEVEF
                     TQPGYAIEYDFFFPYQIYTTTETKLIEGLYFSGQVNGTSGYEEAAAQGLMSGINAALK
                     IKNEKPFSLLRSEAYIGVLMDDLTTKSTEEPYRMFTSRAEHRLYLREDNADERLFKYG
                     YKFGLIAEEHYQKFLEESAEQKKHRQSLKRLFIEVKKLPADFLNNGRTKITFEKALKV
                     PGVGIDLLKEYDDALAELSGRILKKIEIEVKYQGYLERQMREIEKFDKLEHERIPDNF
                     DYAFCKGLKTEAAEKLNRLRPATVGQASRISGISPGDITVLTIYLKKFKEGLRVALKE
                     KEH"
     gene            10139..10777
                     /gene="rsmG"
                     /locus_tag="ENO07_04300"
     CDS             10139..10777
                     /gene="rsmG"
                     /locus_tag="ENO07_04300"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDS01221.1"
                     /translation="MIFRDDIETYLHQFGVEYSGEILAALDSYARLVEKFNASLNLVS
                     KTDTGSEIVKQIIDSSALTKFMSPEKNSTLLDIGSGAGFPGIVLKIFWPEIHLISLDS
                     SPRKITFQDEAASKLNLRDCEFVNLQFQEYRPEREIDYITVKALGNFKKVLAFAGRAL
                     REEGSLIFYLGETVPEELKEIEDAPFRLGSENLYSLPNYPGDLRFLKLIKTV"
     gene            10855..>11039
                     /locus_tag="ENO07_04305"
     CDS             10855..>11039
                     /locus_tag="ENO07_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01222.1"
                     /translation="MTRVIAIANQKGGVGKTTTAINLSACLAVAEKSVLLIDFDPQAN
                     TTSGMGLDKNNLPKTIY"
BASE COUNT         3031 a         2614 c         2698 g         2696 t
ORIGIN      
        1 acacatgatt atatgtatat cgggccggac agcatggttg tgatgaaccc ggtggtatat
       61 tctcctgaat cagacagcat gatacaaatg aggatttttg aagtcgatga cagccttttt
      121 gcgaagactt cagggctgga cgaggttgtc ctggattcga tcgggggacg caggccccgt
      181 attatcatcg gcgggacagg ggtcagcgga aaccagttca tcgacaacaa ggacaaacgg
      241 ctctggctga atgaaaaatt tgatgctctg attgtcgaca tggaatcctc ggccatcgca
      301 cagacatgtt atgtcagcga ggtacccttt atcatcttcc gttcagcttc ggacttggcc
      361 ggcggagccg agaccgagac cgccggtgaa cagctggaaa gatttctcga ggtggcagca
      421 gacaattcct cgagcgtggt gatcaagttt ttggaggcgc tctgattgaa tgaaaccttc
      481 gagcagcctc ttgatcgttg aacggggttt tgattcggcc tatggccgaa gtgtgaccct
      541 gctcctgacc gcatactcca caagaccgct acctgttaga cagaatcacg accgagattt
      601 tcgacatgga aagcaattgc ttgtatcaag ggtgttacgg agagtatctg gagagttcta
      661 gtaatcatta acaggtacaa atgcagtcct ttcctgtgac tgaccaaata ttaaaaactc
      721 cgccagattt cgtagatacg atttgaataa tacatttgga aaactgtctc gaatcggaaa
      781 tccaattcag atgttatatt aatccagatt taatatcaac ccgccggaag cgacttgtga
      841 aaatgaacga tcttccactg accatcctgt ctctgtaaaa cccatgtgca tctcaccggg
      901 agattcatgg tgtccgcacc cgcagttgca tgaaactgcc acagcgatgt cgcccaggct
      961 aaatcacctg atggactgat gtgtatcctg ggatcatttt ccgcaatttg aatattatcc
     1021 agcatttcgt tctgatttgt cataacaatc tcaagatcat cccggcccct gatcggttcg
     1081 ccgaaggctc cgaaattcac catttcggga tcatgaccaa tgtttttggc ataatctgcc
     1141 atgttttcgt tttcaacgct gcttatatag gcaacgagaa catcaccaac cgcttctttt
     1201 tcagccgctg tatctactgc agcttgctca tcctcattct cgcatccggg aagaagaagg
     1261 ctgattgcaa gcagcatagc aaaatacctg agtaacatat tacacctcaa atgagaaaaa
     1321 tcctggagcg aaagcgttgt tgaagatcag atattttaag taattcagat gtgccttgat
     1381 tgtcaatata taatatgact ccgacggcgc accgggaggt acgccgcgca aaaatatatg
     1441 cctacttcaa aatccggctc agatggccat ctgcagaacg taataactgg taccccacat
     1501 gaaatgtggg tataatcctg ctcctgctat gatgatttcc agaaaaagga ctcacgagat
     1561 cggtaacagt ccatgtttta aatcagaata tttcacgcgc tgccttaagc tccaactgca
     1621 aggaacataa ccctgaggca ttatccaatc caataattaa attacttgct gagtagagag
     1681 ttgtcgatat gtgactattc catcatctca ataaactcga ccggtatttc cttggttaga
     1741 taggtatttg actcacggaa aaatgttatt ccggacttat gtgcatcttc tgcgcgaatt
     1801 ttcaagaccg ccacattctg cgaatgcctc cgtccaacat tacgcgcatc ctcaatattt
     1861 gtactcaggt gcacatactg ccgccgcatc ggccttaggc ctttggaaaa tatgagccca
     1921 agattccttc tggctgtgcc gtgatataga acttccgggg gcttcgaggg gatttcacgc
     1981 gactccactt taatcgaatg tccgtaacga gcccttatac gatcattttc aatctgatag
     2041 cgttccttgg aatcagtctc cacaatatat ttcagatcat ctaccgtgag gccaggattt
     2101 tttctctgca tgattccgac cacgtcgtga acctgcgccc atccatggtt gtcaagccta
     2161 agattaaact catcggctcc atgccgcaaa agaaacgcca gaaatttgct ttgcttcttc
     2221 gtcatatctt acatcattaa ctatttgaat gcgtggcacg cacaaaccca ctttgggcgt
     2281 gatataatac catgcactgg tccaagtttg aacgactact cggatcagga aaaccccgcg
     2341 gcgcatcagg acgtacgccg cgcaaaaata tatgcctact tcaaaatccg gctcaaacga
     2401 ccatcagcta attgcagcat attcttcgct gtcttcaggt cggtccgctt gaaatgcatc
     2461 acgatggcag acttgacatg cccctcggcg cgctccagga gcttcttagc ctgactgtaa
     2521 ttcagatcgc agaccgtaat cagagtttta atcgatcgct gtaccagctt ctccgaagtc
     2581 gcctgcagat caaccatcaa atttccgtat gtcttgccga tttttaccat cgcggttgta
     2641 gtgatcatat tcagaatgag cttggtggcg gttccggcct tcattcgggt cgagccggca
     2701 atcgcctccg ggccggtgat cggattgatg ataacatcag gcttaaacaa aagcctgact
     2761 ttgcggttgc agcacaaaaa gactgtgctc gcacgcatct tttttgcctg cttcagtcca
     2821 gccaatgtgt acggcgtccg attggaggta gcgatggcaa tcacagtatc tttcgacgag
     2881 acatcttttt tctctatatc gcgcctgccg gcctcagcat catcctcgat accttcctta
     2941 gacctgacca gcgttcgcct gccccctgct attattccct gcaccagcga cggtttcact
     3001 ccgaatgtgg ggggacattc ggaagcgtcc agcaccccca gccggcccga ggtacccgcg
     3061 ccgatatagt agagatgccc gccggaccta aaagacttaa ccacaagctc gaccgccttt
     3121 tcaacctgcg gaattacctt tctgactgcg ggggctacct gatgatcctc gacatttata
     3181 agctcgagca ccttgcgggt ggacaatata tcaatagacc gagtccattt gttcacggac
     3241 tcggtcgcta agtcctttat ctcgttaaat actttctttg acatcagggc tgtgctataa
     3301 ccgtaaccga atcgtagaat gaaatgatgg ccgaacccag ccttccctta acgtcaacca
     3361 gatcgatgtt attcgcgaat ccggtgtcgg gatagggaaa gaatacccgg tcatcattct
     3421 caataagagc atcggaatag aaagtttcgg aataggccac tacatatatt ttatatattc
     3481 ctgtcaccct ctggccggtg ctggtggtga aagcctcggc gccaatcaca aattcattgg
     3541 tggagttggc aaattcccag aatggggagg cggcatcaga aggcggctca atagtcacaa
     3601 aatagccaaa ctcagcgctg ggcggatccc actcaattga aactgtcccg cccgaataaa
     3661 ttctcgtggc gggcctggta accaccacgc taaatgtatc cgcgatatcc agcgttttgc
     3721 tgaaatagaa atctcccaga tcctccccgg cccagaagac atagctggaa tcgggggcga
     3781 tattcagcat accctcattg gtgaggtaag ctccccctcc aatcgggaca agctgggtgg
     3841 aatcctcatt actggtgaca agaaaacttg tcagggccgg tatcggttcg ccatcttcca
     3901 ggataaaggc ataggcaaaa gaggtgttgt cattgggatt gtgaagcagc agcccattaa
     3961 cagcaaactc gctatcaagc tcaccgtcga cggtgccctc acagccggcc aggaccagag
     4021 cacctatcag gaagattgag attgatactt ttctgagcat aatatgtccc ttcatttata
     4081 ttctacttaa attatcgtaa agatttccaa aattttatag ttaatagatt ataatgtcaa
     4141 gaaaaagaat tgaaaccgct tgacaaaagt ccggtttttg ctattataag tggtttttaa
     4201 agaagaggtg aattgtgaag agaacatatc agccatcgcg aagaaaacgc ctgaacgatc
     4261 acggcttccg aaaaaggatg tctacaaaaa atgggcggaa ggtcctcaaa agacgccggt
     4321 caaaagggcg caagcgcctc acgccgcaga tcaccaagaa ataagggtga atatagtttc
     4381 ggcagagaat attctctcaa ggaagacctg gcgattaaga gggttttcag atccggcagg
     4441 aaatttgaag gcagatgcct ggtcatatat cattatggtg ccggtagtct gcctttaaaa
     4501 gttgccctta aagttagaag gaacattgga tcatctccaa gaagaaacag gattaagagg
     4561 ataataagag agatcatacg cactaataaa aacaaattca agcacggggg acatttggtg
     4621 atttcggtca tagttgatcc cggcgatgag actctctttg aagcgataga aaaggattta
     4681 tttgattttg cgcaggctcg gtaaggttct ggcatatccg ctaattttga tcatcagaat
     4741 atatcaggtg gcgatatccc cactttttcc gactcaatgc aggtttcatc ccagctgctc
     4801 caattatgct attgaagcct tgaataaaca tgggcttaaa ggtatattta tgtccataaa
     4861 gcggatttta aagtgtcatc ccttttcgaa gaagagcgga tatgacccgg tgaggtaacc
     4921 ctatggatcg caaaacagta ctcgcattaa taattatcgg aataatcttt gtcggatgga
     4981 tatatctgac ccagccggag ccccgtcctc agccggttga aacacccgtt gaaaccgaca
     5041 ctctgggagt tagagaggcg actggcgatg aggcgcaaac gtttgaagag caggtagata
     5101 cagccgtagc ggagggtttc gagccgattg ggggcgattc attatatgcc gcattcgaca
     5161 cccttcgggt cgatacagta caggtggaaa ccgataaata tatagccttg ttctcctcgc
     5221 gcggggccgg tctggtcagc ttcaaattca agaactataa ttataataat ggcgatacaa
     5281 ccatggtcga gatggtaccg ccctgggcag aagcggcatt aaaattccag tttcctgacg
     5341 cccgggacag cttctatttc aaccgcattg tctttatgcc cgaaaaaaac aatatcaggc
     5401 tttccggggg aaataaggac aaactgacat ttaccgctaa tcttgcaaat cagggctcca
     5461 taatggttga atacgaattc tacgccgacc gctacgactt ttcggtccgg ctagacttcc
     5521 agaatgcgtc agccttcgat ctggggcgct attattattt cggatgggag ccggggcttg
     5581 aatcaacaga aaaaaaccgt caggacgact tcaacagctt caaagcttat gtcatgtggg
     5641 atacaggctt agagggttac agcaagttcc agaatggcca gatgtcacag gaaatcgagg
     5701 ggaatctgca atgggtggca accaaaacca aatatttctt cgtcggcata gcccccgacc
     5761 gcgctcccca aggcgtcaga atacagggac gcgaaatcag tgtcaatgag aaagccggtc
     5821 agccggggat gaagaaaata ggtgtgcagg cagagatgga aatccaccag cgcaaatccg
     5881 atctatttga caaatatctg atttacgtcg gcccgatcga ttataaggtc ctgagaagct
     5941 acaatcgcgg cttcgaggag actgtcgatc tcggatggcc gattataaaa acaatctccc
     6001 gctttattct gtggttgatg gaagtgctct atggtatatt ccataattac ggcgttgtaa
     6061 ttattatctt cagcatcctg atgaaggtcg tattctaccc gctgacatca cgaagtctca
     6121 ggtctatgaa gaagatgcag gagttgcagc ccaagctcaa ggcgctccag gaaaaataca
     6181 aaaaagagcc ccagaaaatg caggccgaga ttatgaagct ctggaaggag aacaaggtca
     6241 atccgatgag cgggtgtctt ctgatgcttc cgcagcttcc catatttttt gcactcttca
     6301 cggtattcag gaataccatt cttttgcggg gctccgaatt catgctctgg atgaaagatc
     6361 tctctcaacc ggaccagacc atgattctac ctcttatcat ggcggggacc atgttcctgc
     6421 agcagaagat gaccatgcag gatcccaagc agaagatgct cgtgtatatc ctgccgatag
     6481 tatttttctt cctgttcaaa ggctttccta cagggctggt gctgtactgg acgatgttca
     6541 atgtgctctc ggttctggaa acgctcctta taagaaaacc acagcagcaa aaagaattag
     6601 cgcttaaaac tgaatgatat catatagcga agatacaatc gccgccgtct ccacacctcc
     6661 cggagagggc ggtatcgccg ttgtgcgtat atccggaaaa tcctcgaagc agatcgcaga
     6721 caggatcttc cggggcagaa taacgccctc tgaggccggc agccatacgg tgcacttcgg
     6781 gaagatagtc gatcccgaca gcgaggagct cgtggatgag gttttgttaa ccgtaatgtg
     6841 ggggccgaat tcttataccg ctgaagatgt tattgaaatc agttcgcatg gcgggcagct
     6901 ggttgcaaaa aaaatactgg atctgtgcct gaaaaccggg gcgcgtcatg cccttccggg
     6961 tgagttcaca cagcgtgcct tcctgaatgg aactatcgac ctggcgcagg ccgaggcggt
     7021 ggctgatatg atctcggccc gtaccgatat gagcctcagg tcagcagccc ggcaattggg
     7081 aggaaatgtg tcccagacag tagcttctct gggcaataaa atcagaaatc tccttgccct
     7141 tctggaagcc tacaccgatt tcccggaaga ggagatgggg catgctgaca tacaaaaact
     7201 gactgatgac cttaatatca taaaggatga aataaacaga ctggtattgt cttatgagga
     7261 aggcaaagtc ctcaagagcg gtttgaagtt gcctattgtg ggccgtccca atgtgggaaa
     7321 atcgtcgctc ttcaatcgac tcctggacga aagccgtacg atcgtgaccg aaattccggg
     7381 cacaacacgt gatactgtct cggagtttat caatattgca ggcattccgg tggaattgat
     7441 cgacaccgcc ggcatccgta ccaccggaga tgtggttgag gcggaaggca taaaacgcgc
     7501 cagaaaagag atagagaatg ccgacctggt cctggctttg gtggatatga cagatcccga
     7561 gattctggat gatatcagga gcctgactga ggcgctgggc tcggtcaatt atattctgct
     7621 ggcaaacaag gcggacctga tagatgagac tatgctggag gcccgcaaaa tctccctggc
     7681 tgagtataac ccgctgccgg tttccgcaga gaccgggatc ggtcttgagc agcttcgaga
     7741 cttgatcatc aaatctgccg gcatagtgaa aaaatccagt ctcaaagacg agactattat
     7801 cacaaatgtc cgccatcgcg atgcccttag caaggggctc ggatttctgg ataaggtgga
     7861 gcaggggctg gaagagaagg ccagctatga attcctggca tttgatctga agaatgctgt
     7921 caacgcattg gaagagatta tcggcaagac tactccggaa gatatcttaa acaaaatatt
     7981 ttcgcaattc tgcatcggta aataattgtc atgccaacta aaatcaaaat gtttcacgtg
     8041 aaacattaag ctttatagaa aaattttgag cttgtcacaa actaaaaaca ggctattttc
     8101 tgtatcatat actaataaga tatgacaaat ctgcattatg aaatagttgt tgccggcgga
     8161 gggcatgccg gagcggaggc cgccctggcg gcgtcaagaa tgggatgttc caccctgctg
     8221 gtgagcctca ggaaggacac gatcgggcag atgtcctgta atcccgctat cggggggcag
     8281 gccaagggac atctggtgaa ggaaatcgat gccctggggg gtgagatggg tctggcgatt
     8341 gaccgcaccg gtatccagta ccgcaggttg aatctctccc gcggcccggc agttcattcc
     8401 tcgcgcgcac aggccgaccg taaattatat cgcgaatata tcaccggcgc ggtcctgaat
     8461 cagggcaacc tggattttct cgaggatgcc gtggttgatc tggaagtcaa tggcagtcat
     8521 gtcagtgcgg cgataaccga gggcggcaga aaaatcaact gccgatgttt aattataaca
     8581 gcagggacat ttctaaatgg cctgattcat atcggcagaa aaaagattcc ggccgggcgg
     8641 atgggcgaga aaccctctct gggattatcg gaaaagctga ttgccctggg attcaaaagc
     8701 ggccgcctga aaacaggcac cccgccccgt ctggatggaa aaacgataga ctactccaga
     8761 accgaatcgc agtatggcga cccggatttt ccgccttttt cattcaggtc gaattccata
     8821 aatggcaata aagctatttg ccatataact ttcacaaatc aagatactca tcggattatt
     8881 ctcgataatc tggattcctc ggccatgtac tccggacaga tcaagggtat cggtccgcgc
     8941 tattgtccct cgattgaaga taaggtcgtc agattcaggg ataaggaccg ccaccagata
     9001 tttctggagc ccgagggatt ggatacggac ctgatatatc ctaacggttt cccgaccagc
     9061 ctcgatgaag aagtccagaa gaaggcgatc aaaacggtca tcgggctcga ggaggtggaa
     9121 ttcactcagc cgggatatgc gatcgaatac gatttcttct ttccctacca gatttatacc
     9181 accaccgaga ccaagctgat cgagggactg tatttttcgg ggcaggtaaa cggtacctcc
     9241 gggtacgaag aagcggctgc gcaggggctg atgtcgggaa taaatgccgc tcttaagatt
     9301 aagaacgaaa agcctttcag cctgttacgt tcggaggctt atatcggtgt gctgatggat
     9361 gacctgacca ccaaatcgac cgaggaaccg taccgtatgt tcacctcacg cgccgagcat
     9421 cggctgtatc tgcgcgagga taatgccgat gagcgccttt ttaaatacgg ctacaagttt
     9481 ggactgattg ccgaagaaca ttaccagaaa ttcctggagg agagcgcgga gcagaagaaa
     9541 catcgtcagt ccctaaagag gctatttatt gaggtcaaga aacttccggc cgatttcctg
     9601 aataatggcc gcaccaaaat caccttcgaa aaggcgttaa aagttcccgg ggtcggtatt
     9661 gatctcctga aagaatacga tgatgccctg gcggagcttt ccggccggat attgaagaag
     9721 atcgaaatcg aagttaagta ccagggctat cttgagcggc agatgcggga gatcgagaaa
     9781 ttcgacaaac tggagcatga gaggattccc gataatttcg actatgcctt ctgcaaaggg
     9841 ttgaagaccg aggcggccga aaaactgaac cgcctgcgcc ccgcgacagt cggccaggcc
     9901 tcacgaatat ccggcatatc gccgggagat ataacggtat tgactatata tctgaagaaa
     9961 ttcaaggaag gattaagggt agcgctaaaa gagaaagagc actgactttt tatatctgtc
    10021 attttgagcg aagcgaagaa tctgtttgat tctaaagaga ttcttcggtc gctgcgctcc
    10081 ctcagaatga ctggatcatc aacacagatg tttcacgtga aacattgaca ggaagaacat
    10141 gattttcagg gatgatatag agacctacct tcaccaattc ggagtggagt attccgggga
    10201 aatactggcg gcgctggatt cctacgcccg cctggtggag aagttcaatg catcgctcaa
    10261 cctggtttca aagacagata ccggaagcga aatcgtcaag cagattatcg acagctcggc
    10321 gctgacaaaa ttcatgagtc ctgagaagaa ctccaccctc cttgacatag gcagcggcgc
    10381 cggctttccg ggcatagtac tgaaaatatt ctggccggag attcatctga tctcgctcga
    10441 ttccagtccc agaaaaatca cttttcagga cgaggccgcg tccaaattga atctgaggga
    10501 ctgcgaattt gtgaatctcc agtttcagga atacagaccc gagagagaaa tagactatat
    10561 tacagtcaag gctctgggca atttcaaaaa agtgctcgca tttgccggca gggcattgcg
    10621 cgaggaggga agcctgatct tctacctggg agagacagtg ccggaggagc tcaaagagat
    10681 cgaagatgcg ccattcagac tgggatcgga gaatttgtat tcgcttccca attatcccgg
    10741 cgatcttcgc ttcctaaaat taattaagac tgtttaaaaa attcttgcat taattataaa
    10801 acattctttt aatattcctg ctcaaagcta tatgtataag agggacattt ttgtatgacc
    10861 agggtaatcg caattgcaaa tcaaaaaggg ggtgtgggta aaaccaccac ggcaatcaat
    10921 ctttccgcct gcctggctgt tgctgaaaaa tcggtacttc tgatcgattt cgatccccag
    10981 gccaatacga caagcgggat gggtctggac aaaaacaatt tacccaaaac gatttatga
//
LOCUS       DSEB01000240            2148 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1008650, whole genome shotgun sequence.
ACCESSION   DSEB01000240 DSEB01000000
VERSION     DSEB01000240.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1008650"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..870)
                     /locus_tag="ENO07_04310"
     CDS             complement(<1..870)
                     /locus_tag="ENO07_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01223.1"
                     /translation="MTQPEAINSGVQLSDAEKREIANLQALGRDLATQLYVCLKTASF
                     YDPRNDNFKKQISKFLDLLHVATDHESHLSITDVDGYLFLNDQRLKIKLDGYLAAKYL
                     QSQFENLGVAGMEFSVKTELEELEKAFSQVVRSDLKVRNAENLNDYFRGLEIKNIKFI
                     PSLTIDDCRDDDDDESERTSAKKTFFQALSVVQQVMGGFAQQKKRNTNFFKAKRAIHG
                     IIDEIIKNESYMLELTVLRNYDQYTYKHSVNVSVFAVALGIRLGLSKARLAELGFAAL
                     FHDFGKTRIPIDLL"
     gene            complement(870..>2148)
                     /locus_tag="ENO07_04315"
     CDS             complement(870..>2148)
                     /locus_tag="ENO07_04315"
                     /inference="COORDINATES: protein motif:HMM:PF13646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HDS01224.1"
                     /translation="GFSAPEQQEVEYSEIILSESQVETKNALDALLDGSEKELAGLSE
                     DDDFLGIFRNIQEFGPSEKAYLEDLMAADGELIIEFESIELLLDILLSEDELGNFDES
                     LLTLDNMFDKMLDSEQFPLLVYLIQGMKKCYAELQKKSGPRADKFKDSLARVGDRIRI
                     SRITNLLNSSEWQDFDGIRLYLEELEWKSMPSLIWMLGELNNFPARKMVIEALINKGR
                     DRIEIIGNAIHDSRWYVVRNAVLILGEIGDNKGLSYMRKALEHFDERVRWEAVVAVEK
                     LASKSDLKILIPYLKDESERIRHKVIEMFSQNRYDPAFEAIQEIVKSSQFNGMGFEEQ
                     KNLLKALAFTGGDNALPVLKKIVKKRSFFSARASQKKKEAAVLAISLIETEDAWKLIE
                     EAANKKKGPLANLAQLILDKKGRVAADRNTEGD"
BASE COUNT          499 a          577 c          462 g          610 t
ORIGIN      
        1 gaggaggtcg atgggtatcc tggtcttgcc gaaatcatgg aaaagggcgg cgaatcccag
       61 ttccgccagc ctggctttgc tcaatcccag tcggataccc agcgcgaccg caaacacgct
      121 cacgttaacc gaatgtttgt aggtatattg atcatagttg cgcagaaccg tcaattcgag
      181 catgtaggat tcgttcttga tgatctcatc gatgatcccg tgtatggctc tttttgcttt
      241 gaaaaaattt gtgtttctct tcttctgctg ggcaaaacct cccataacct gctgcaccac
      301 gctcagggcc tggaagaatg tcttctttgc gctggtgcgc tcgctttcgt cgtcatcatc
      361 atcgcgacaa tcatcaatgg tcagggaggg gatgaatttg atgtttttta tctccagtcc
      421 cctgaagtag tcgttgaggt tctcggcgtt tctgaccttc agatcagatc tgaccacctg
      481 agaaaatgcc ttctccaatt cctcaagttc ggtcttgacc gaaaattcca tcccggctac
      541 gcccaggttc tcgaattggc tctgcaggta ttttgccgcc aggtacccgt caagtttgat
      601 cttcagcctc tggtcattca ggaataaata cccgtcgaca tcggttatgg atagatggga
      661 ttcatggtcg gtggccacat gcagcagatc caggaatttt gagatctgtt tcttgaaatt
      721 atcgttacgc ggatcataga atgaagcggt cttcaggcag acataaagct gggtggccag
      781 atcccgtccc agagcctgca ggtttgcaat ctctctcttt tcagcatcgc tcaattgcac
      841 gccgctgttt attgcttctg gttgcgtcat taatctccct cagtgttcct atctgcagcg
      901 acccgccctt tcttgtccag gatgagctgg gccagatttg ccaatggtcc cttcttttta
      961 ttggcggcct cttcgataag tttccaggca tcctcggttt ctatcagaga gatagccaga
     1021 acagctgctt ctttcttctt ctgagaggcc ctggctgaaa agaaagacct cttctttact
     1081 atcttcttga gaacaggtag tgcattatcc ccgccggtaa aggccaatgc tttcaaaaga
     1141 tttttctgct cttcaaagcc catgccgtta aactggctgg acttgacgat ctcctggata
     1201 gcttcaaagg ccggatcgta gcgattctgc gaaaacatct caataacctt gtgccggatc
     1261 cgttctgatt catctttcaa atagggaatc agtattttaa gatcggattt cgaggccagt
     1321 ttttcgaccg ccaccaccgc ttcccagcgc acgcgttcat caaaatgctc cagcgctttg
     1381 cgcatgtagc tcagtccctt gttgtctccg atctcaccta aaatcaacac cgcattgcga
     1441 acaacatacc agcgtgaatc atgaattgca ttacctataa tctcaatcct gtccctgccc
     1501 ttatttatca gggcctcgat caccatcttc ctggccggga aattgttgag ctcgcccagc
     1561 atccagatta gcgagggcat gcttttccat tccagttctt ccaggtatag ccgtatgcca
     1621 tcgaaatcct gccattccga agaattcagg agattggtta tgcgggaaat cctgatcctg
     1681 tcccccaccc tcgccaggga gtctttaaac ttatccgcac ggggcccgga ttttttctgt
     1741 aactccgcat aacatttctt catcccctga atcagataaa ccagaagggg aaactgctca
     1801 gaatcaagca tcttatcgaa catgttatcc agtgtcagca gactctcatc gaaattcccg
     1861 agctcatctt cagacagcag tatgtcaagc aaaagctcga ttgactcgaa ttcaatgatc
     1921 agctccccgt cagccgccat gagatcttcc agataagctt tttcggacgg gccgaattct
     1981 tgaatgttcc tgaatatacc caggaaatca tcatcctctg ataaacctgc cagttccttc
     2041 tcggaaccat cgagcaatgc atccagagca tttttggttt caacctgaga ctcggataat
     2101 ataatttcgg aatattcaac ctcctgctgc tccggagcgc taaatcca
//
LOCUS       DSEB01000241            3131 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003761, whole genome shotgun sequence.
ACCESSION   DSEB01000241 DSEB01000000
VERSION     DSEB01000241.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003761"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            24..1115
                     /locus_tag="ENO07_04320"
     CDS             24..1115
                     /locus_tag="ENO07_04320"
                     /inference="COORDINATES: protein motif:HMM:PF00496.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01225.1"
                     /translation="MPILFAHYQGEKEPEYDTLSKFLISSYHIYEIDNFEVDTTHRVS
                     SGPYRIQNLNQNEILLLKNRYYRGSAKFPDTILIKIEPDYLKRKLMFQLGEIDMLDLH
                     FSDIDQFKGDYEIKEVVLDEAAYLSINNIKEYLSDGVLAAALSYLINKDAVCRVALNK
                     SAEMIDNLLPSWAPVAEPMYAYNKSKGRSLIRNMGGVNRYLSLYVSPDDFWGRRMAEY
                     IKGILERENIYVTIYSELLEGEGRDYDLFQQFDMMIGRIDLFNDHPVYIMNQMVFHYD
                     LADISKNRSLHYSPEHDSIMTAYFENPSENEDMLKEYYRNLVEFPVGIPLFKPIRQTA
                     ISGRIKNIEFNDQGLIDFSTIEIEDETQR"
     gene            1099..2334
                     /locus_tag="ENO07_04325"
     CDS             1099..2334
                     /locus_tag="ENO07_04325"
                     /inference="COORDINATES: protein motif:HMM:PF00672.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDS01226.1"
                     /translation="MKLKGKLSLYFVLATGLPLIAAVLILYFYLIGGLNRIETDNIRA
                     ALKSVKTHFEREGDNLSQRVARALRADDYQLLQILLTKNRRGQIDQSKLIDLTGEYQN
                     LLKLDFLEIVSPDGTILASGIRPTDFNRPTDFEFFDLINENGSVTGFTSYRVGEDKYL
                     SYINGQVLTYNDSTVAIFIGGIFLDDYYLREMQLPYNVQSAVLSGRELYASTFDPNLE
                     ELLNLELKNRMAEEFTDDFALGGVDYSPYYQLLYSLNDDIRYQIMLLYPPSYARLLTA
                     QSFRIYAVVAIAGILIAGLLGYAFAQKLSAPIMELARASEKISQGKFDRKIIWFSNDE
                     MGTLVDGFNEMYDRLKRSQQKLIQAEKLAAWNQMARKVAHEIKNPLTPIKISIEDLRR
                     SYQKNEKDYDHILENASST"
     gene            2334..2840
                     /locus_tag="ENO07_04330"
     CDS             2334..2840
                     /locus_tag="ENO07_04330"
                     /inference="COORDINATES: protein motif:HMM:PF02518.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDS01227.1"
                     /translation="MNEIDKLKRIVDEFSSFARLPSPELSQIRVVDLVKASLKLYDNL
                     LQEGRLIIKTEKDPGNVLADKGLFSQALVNIVKNAIEADPEGRIEVTVSREEDQALIS
                     VRDHGPGISEKIRAQMFTPYFTTKAGGSGLGLVITQRILFDHEADISVNSEEGKGTEF
                     IIRMPVVK"
     gene            2842..>3131
                     /locus_tag="ENO07_04335"
     CDS             2842..>3131
                     /locus_tag="ENO07_04335"
                     /inference="COORDINATES: protein motif:HMM:PF00072.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDS01228.1"
                     /translation="MSAKILIVDDEESILASLSGPLEREGYEVITCENAARAKMENGR
                     DVDLSLLDVWLPDGDGIELLEQFRKLYPLQGFIMMSGHSDIETAVKAVKMGA"
BASE COUNT          856 a          718 c          759 g          798 t
ORIGIN      
        1 atcagtgttt atccactgtt aatttgccca tattatttgc acattatcag ggagaaaagg
       61 aacctgagta cgatacgctc tctaagtttc tcatctcatc ataccacata tatgaaatcg
      121 ataatttcga agttgacacg acccacagag tgtccagtgg gccatatcga attcagaatc
      181 tcaaccagaa tgagatacta ttattaaaaa acagatatta tcgcggttct gccaagttcc
      241 ccgacacgat cctgatcaaa atcgagccgg attatctgaa acgaaagctg atgttccagc
      301 tgggcgaaat cgacatgctc gacctgcatt tctcggatat cgatcagttc aaaggcgatt
      361 atgaaatcaa agaggttgtt ctcgatgagg ccgcatattt atcgatcaac aatatcaagg
      421 aatatctttc ggatggagtt ctggcggcgg ccctctccta tttgataaat aaagacgctg
      481 tatgccgtgt tgcgctgaat aaatccgcgg aaatgattga taatcttctg ccctcatggg
      541 cgccggttgc ggaaccgatg tacgcctaca acaaatccaa aggccggtca ttgattcgta
      601 atatgggcgg cgtgaaccgc tacctttcac tttatgtcag ccctgatgac ttctggggac
      661 gtcgcatggc cgaatatatt aagggtattc tggaacgcga aaatatctat gtgaccattt
      721 actcggaact tctggagggt gaggggcgcg actacgacct tttccagcag ttcgatatga
      781 tgatcggaag aattgatctt ttcaacgatc acccggtcta tatcatgaac cagatggtat
      841 ttcactacga cctggcagat atcagcaaaa accgcagcct tcactactca ccggaacacg
      901 acagcattat gaccgcatat ttcgagaatc catctgaaaa tgaagatatg ctcaaagaat
      961 attatcgcaa tttggtcgaa ttcccggttg gaataccgct gttcaagccg attcgacaaa
     1021 cagcaatatc aggtcgtatt aaaaacattg aatttaacga tcaggggctg atcgacttct
     1081 ccaccatcga gattgaagat gaaactcaaa ggtaaacttt cattatattt cgtcctggcc
     1141 accggccttc cgcttattgc cgccgtgctg atcctgtatt tctatttgat cggcggcctg
     1201 aacaggatag aaaccgataa tatccgcgcg gccctgaaga gcgtgaagac ccatttcgaa
     1261 cgcgaggggg acaatctttc tcaaagagtg gcgcgtgcgc ttcgggccga tgattatcag
     1321 cttctgcaaa tactcttaac gaagaaccgg cggggccaga ttgaccagag caagctgatt
     1381 gatctaaccg gcgaatacca gaatcttctc aagctggact ttctggaaat agtatctccc
     1441 gatggaacaa tactggcctc cggcatccgt cccaccgatt tcaatcgtcc cactgacttt
     1501 gagtttttcg atctaatcaa tgaaaatggt tctgtaaccg gatttacttc atacagagtt
     1561 ggtgaggata aatacctgag ttatatcaac ggccaggtcc tgacttacaa tgactcaact
     1621 gtggcgattt ttataggtgg catcttcctt gacgattact acctgcgcga gatgcagctg
     1681 ccgtataacg ttcaatctgc ggtgctgtcc ggaagggagc tttatgcctc gacgttcgat
     1741 ccaaatcttg aggaattact gaatttggaa ttgaaaaaca ggatggccga ggaattcacg
     1801 gatgatttcg cgcttggcgg ggttgattat agtccttact atcaattact ttacagcctt
     1861 aatgacgata tccgttacca gattatgttg ctctatcccc cctcctacgc ccgcctcctg
     1921 accgcccaga gcttcaggat ttatgctgtt gtcgcaatcg caggaatcct gatcgccggt
     1981 cttctgggat atgccttcgc acagaagctc tctgcgccga tcatggaact ggcccgggca
     2041 tccgagaaaa tttcacaggg caaattcgac cgcaagataa tctggttctc caatgatgag
     2101 atgggtactc tggtggacgg cttcaatgag atgtacgacc gtctgaaaag atcacagcag
     2161 aaactgatcc aggccgagaa gctggcggca tggaaccaga tggcgcgcaa ggtggcgcat
     2221 gagattaaga atcctttaac gcccataaag atttccatcg aggatttgcg ccgcagctat
     2281 caaaaaaatg aaaaagatta tgatcatatt ttggaaaacg cctcttccac ttaattaatg
     2341 agatcgataa attaaagcgc atcgttgacg agttctcatc atttgcgagg ctgccctccc
     2401 cggagctgag ccagatacgg gttgtggatt tagttaaggc ctcgttgaag ctgtatgaca
     2461 atctgttgca ggaaggcagg ctgatcataa aaacggaaaa agatcccgga aatgtcctgg
     2521 ctgataaagg tcttttctcg caggctctgg ttaatatagt gaaaaatgcg atcgaggctg
     2581 atccggaggg cagaatcgag gtgacagtca gccgtgaaga agatcaggct cttatatcgg
     2641 tcagggatca tggcccgggg atttcggaaa aaattcgcgc gcagatgttt accccgtatt
     2701 tcaccaccaa ggccggtgga agcggattgg ggctggtaat tacgcaaaga attctgtttg
     2761 accatgaggc cgatatttcc gttaatagtg aggaaggaaa aggaaccgag tttataatca
     2821 gaatgccggt ggtgaaataa catgtccgca aaaatactta ttgtcgacga cgaagaatcg
     2881 atcctggcct ccctctcagg gccgctcgag cgcgagggct acgaagttat tacatgtgag
     2941 aatgccgcga gagcaaaaat ggaaaacggc cgtgatgttg acctgtcgct tctggatgtc
     3001 tggcttcctg atggtgatgg gatcgagctt ctggaacagt tcaggaaact ctacccgctc
     3061 cagggcttca tcatgatgtc cgggcattcc gatatcgaga cggctgtgaa agcggtcaag
     3121 atgggcgcgt a
//
LOCUS       DSEB01000242            3179 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003644, whole genome shotgun sequence.
ACCESSION   DSEB01000242 DSEB01000000
VERSION     DSEB01000242.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003644"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..480)
                     /locus_tag="ENO07_04340"
     CDS             complement(<1..480)
                     /locus_tag="ENO07_04340"
                     /inference="COORDINATES: protein motif:HMM:PF00149.26"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDS01229.1"
                     /translation="MSIRFVHMADTHLGAGGFGNKLSKNGINQREEDICKAFSNAVEK
                     TLELKPDFVIHAGDLFHMVRPTNRIINIAIIEILRLTRAGIPVIIISGNHDAPKQRSV
                     GHVLSIFENLDNVFPVYKSKYEVVKMQDVSVCCLPHCLTPEILQEQFEHVRPELPGLN
                     "
     gene            complement(493..2067)
                     /locus_tag="ENO07_04345"
     CDS             complement(493..2067)
                     /locus_tag="ENO07_04345"
                     /inference="COORDINATES: protein motif:HMM:PF01935.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDS01230.1"
                     /translation="MSMMDDFLGRITGRKIGEIYFRLSYSTRIDLGDLAIAEDAETGI
                     KFYLRVVDISYGAEGADLGWFERTAGNLMLLDESEDEYGLFEKERRLYKIARCNLLGY
                     SRDDRFFKPKTIPSHFTRVRRPQGKDFDFLKSLGANLLFSRLRSGESVLDFEIGISSK
                     SVASHIGVFATTGMGKSNLMKRLAGSILENGNVGLLIFDPHGEYFDGGGEAHLKGLIH
                     HAMADQNLRIYSSRPLERRYQKLQISASEIVPEDLNHIYEFSEAQHEALSALSYRFGK
                     NWLSELRASDISELSQRITTQGGFAESTLGVLKRRAESLLKQNYIHTDSEVSTSRNII
                     EKLKDGKVALVDSSNLTTRDELLIGSVLCRAVFHHYRSLYSRPDDFKHVTPILVAIEE
                     AQRVLRKSSGGFANIFARIAREGRKFKIGLCAITQQPKLIDEELLSQFNTFLILGLAD
                     ESDRQIIRSSSKQDISDLGTEIQMLSAGEALITSPEVPFALPVKIDLYEEYLKKAEAH
                     YHKSERKTSSVDEEFF"
     gene            complement(2077..3108)
                     /locus_tag="ENO07_04350"
     CDS             complement(2077..3108)
                     /locus_tag="ENO07_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HDS01231.1"
                     /translation="MDSTVDYIARLLSVDPSLKLLEPAEEDSEPLELENELDEIDYVV
                     IEDSRHEFSAGAVDGGQGTIIRNNVFQAGVYRAGYVKFRGGERTDEYSEKLLMVNLTR
                     ENCVEIYAEAFSHFHGGYPAWIPAFGETLGAMRRLAEEKAIIECLDNLKAGDLLLLDG
                     SLRGDGYGFIQSMIKRAASRQINVASVAKANSLLWRGGANLAAVIRQRGDELHQKKSW
                     YARVGRVAPEKDQSWLGNIYIAKLNPHSGITFRVDLSRSNKKPPSDIFAQLSGLATDP
                     FFLGYPYPLAAVHQMVRLSEDELYGFMMRLQDIALQKGIEQSRWDLLFADYHRILNFD
                     LRENQFRRL"
BASE COUNT          790 a          846 c          738 g          805 t
ORIGIN      
        1 attgagtccc gggagctccg ggcgcacatg ctcaaactgc tcctgaagta tctccggtgt
       61 aaggcagtga ggcagacagc aaacggaaac atcctgcatc ttaaccacct cgtatttgct
      121 cttatatacg ggaaagacat tatccagatt ctcgaatata gaaagcacat gcccgaccga
      181 ccgctgcttg ggggcatcat gattccccga aattataatc accggaatac cggcgcgcgt
      241 caatctgagg atctctataa tggctatatt gatgatcctg ttggtgggac ggaccatgtg
      301 aaagaggtcg ccggcatgaa taacgaagtc cggtttcagc tccaacgtct tctcgacagc
      361 attgctgaat gctttgcaga tatcctcctc gcgctgattg atgccgtttt tggagagctt
      421 gtttccaaag cctccggcgc ccaggtgggt gtccgccatg tgtacgaatc tgattgacat
      481 aatatcggct ttctaaaaaa actcctcatc cactgaggat gtttttcttt cggatttatg
      541 gtagtgtgcc tcggcttttt tgagatactc ttcgtacaga tctatcttga ccggtaatgc
      601 gaacggcacc tcgggagaag ttatcagcgc ctcgccggcg gaaagcattt gaatttctgt
      661 gcccaggtca gatatgtcct gcttggagga tgacctgatt atctgcctgt cgctttcatc
      721 agcaaggccg agaatcaaga aggtattgaa ctgcgacaat agctcttcgt ctataagctt
      781 gggctgctgt gttatggcac acagtccgat tttgaacttg cggccttcac gagcaatgcg
      841 cgcaaatatg ttggcaaatc cgccgcttga ttttctcagg acccgctgcg cttcctcaat
      901 tgcaacgagg atcggcgtga catgcttgaa atcgtcaggc cgtgaataga gactcctgta
      961 atgatgaaaa accgcgcggc ataagaccga tccgatcaac agctcgtcac gggtggtcag
     1021 gttggagctg tcaaccagcg ccaccttgcc atccttcaac ttctcaataa tatttcggga
     1081 tgtagacacc tcactgtcag tatgaatata attctgcttg agaagactct ccgcacgtct
     1141 tttcagcact cccaatgtag attccgcgaa tccaccctga gtcgtaattc tctgcgaaag
     1201 ttctgatata tctgatgctc tcagctcgga gagccagttt ttcccgaagc ggtaagatag
     1261 cgccgagagc gcctcatgct gggcctcgga aaactcatag atatgattca agtcctcggg
     1321 tacaatttcc gaagcagaga tctgcagttt ttgataacgc ctttcaaggg gacgggagga
     1381 gtatatgcgc agattctgat ctgccatagc atgatggatc agtcctttta gatgtgcctc
     1441 tcccccgccg tcgaaatatt cgccatgcgg atcgaagatg aggaggccga cattaccatt
     1501 ctccagtatt gatcctgcaa ggcgcttcat caaattactc ttgcccatac cggtggtggc
     1561 gaagacacct atatgggagg cgaccgattt agaggaaata ccgatctcga aatccagcac
     1621 agactcaccc gaacgcagac ggctgaacaa cagattagcc ccgaggctct tcagaaagtc
     1681 aaaatccttg ccttgcggtc tgcggacacg cgtgaaatgg gaggggatcg tcttgggctt
     1741 gaaaaacctg tcatcccggc tgtatcccag gagattgcag cgggcgattt tataaagccg
     1801 cctctctttt tcaaaaagcc cgtactcatc ctcagactca tccaataaca tcaagtttcc
     1861 cgcagtcctc tcgaaccatc ccagatcagc tccttcagcg ccgtaagata tatccaccac
     1921 acgcaggtag aactttatgc cggtctcggc atcttctgca attgccaggt ctcccagatc
     1981 gatcctggtg ctgtatgaca gcctgaaata aatctcccct atctttcttc cggttattct
     2041 tcccaggaaa tcatccatca tactcatcca ttcttctcac aaacgccgga attgattttc
     2101 ccgcagatca aaattcaaga tgcgatggta atccgcgaaa agcagatccc atctcgattg
     2161 ttctatgcct ttttgaagcg caatgtcctg caaacgcatc atgaaaccgt aaagctcatc
     2221 ctcggagagc ctgaccatct gatgcaccgc cgcaagcgga tagggatagc ccagaaaaaa
     2281 gggatcggtg gcaagcccgg ataattgagc gaagatgtcg gatggcggct ttttatttga
     2341 cctgcttaaa tcaaccctga aggtaatacc ggaatgagga ttgagtttgg cgatgtagat
     2401 attccccagc cagctctgat ccttttccgg ggcgaccctt ccaaccctgg cgtaccagct
     2461 ttttttctga tgcagttcgt cccctcgctg cctgattacc gccgccagat tagcccctcc
     2521 cctccagagc aggctgttgg ccttggcgac agaagccaca tttatctgcc tggaagcggc
     2581 tctctttatc atgctctgaa taaagccgta accatctccg cgcagggacc cgtcaagcag
     2641 tagtaaatcc cccgctttca gattatcaag gcattcaatt atagcttttt cctcggccag
     2701 cctgcgcatc gcgccaaggg tctcgccgaa agcaggtatc caggccggat atccaccgtg
     2761 aaagtgagag aacgcttccg cataaatctc aacacaattc tcccgtgtaa gattgaccat
     2821 cagaagcttt tcggaatatt catcagtgcg ttcgccgccc cggaatttta catatcccgc
     2881 ccgataaacg cctgcctgaa agacattatt tctgataatc gttccctggc cgccgtccac
     2941 agctcccgcc gaaaactcat gccgggaatc ttcaatgacg acataatcaa tttcgtcgag
     3001 ttcgttttca agctcaagcg gctcggagtc ttcttcggcc ggctcaagaa gtttcaggga
     3061 aggatcgact gataagagcc ttgcgatata atcgacagtg gaatccatgc cggcatatac
     3121 atgcgagtaa tcatatagca tagattaagg ataaagattg tttgcttaga gggcaataa
//
LOCUS       DSEB01000243            4227 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001942, whole genome shotgun sequence.
ACCESSION   DSEB01000243 DSEB01000000
VERSION     DSEB01000243.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001942"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            151..2067
                     /locus_tag="ENO07_04355"
     CDS             151..2067
                     /locus_tag="ENO07_04355"
                     /inference="COORDINATES: protein motif:HMM:PF12969.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HDS01232.1"
                     /translation="MSAISFFRSVLLLTSLLFFTFGYLSAQEWGKVEGWEWALEPPPE
                     IPDEPALIIFDRGDMKVDLEGITLMRHVRIKVFDKDNIDDVANIDISYHGDQKFSKFK
                     AHTILPNGDKNKIKDEFEKKAGNLKTVAFTFPSIINGCILEYQYQIFHENFGFLEPWY
                     FQNDIYTLKSIFSVTLYPGFEYATWPINIPAHQQQSSRLHVHEFGRLTTRYTWELDSL
                     IPVKAEPFAGAVSNYTAALHFQIRSFKGTGIRFDFPTKWKEIGIELNKIIDEFIDDED
                     ELEELSGKLTAGALNEDEKIAHIYDYVRDSILTRGSGMLDFSEDKFSNLMDNRYGSAT
                     AKNLLLTALLQKAGFKAHPLLIGTRGFCKFNPAHVQLNQFNHLVCYVNLKIREYLLDA
                     GNRAVAFPYVPASDRIRSGLLIDAEASRPISLLPPPKRKSSTQLKANINLREDGSAAC
                     STFICVQGYENARYDDYLLDTISQQQIATDILDEFELNYDVEEAVSRYFPEGDSLSIK
                     LVIEIPEFASALGDHMLISPFLIPLVNNPFKSEERQYPIDFQYEFTRYHEITINLPEN
                     MIVGDLPDKINRNTDCGKYTRSFLTTGNSIAAILYFKLNKETFLPSEYPGVKEMFDEI
                     TETSKDEIAVIVQE"
     gene            complement(2068..2661)
                     /locus_tag="ENO07_04360"
     CDS             complement(2068..2661)
                     /locus_tag="ENO07_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01233.1"
                     /translation="MRKKNRTVFAALSVIFLLALVMSCGKGLEGKMKAYEEAINSHDV
                     DAVLAFYAEPVTVEMGRVSNPLTETELRPLAEWDSIVNTKVSFTVKETRGDTVFCTKT
                     ETNDWFKAFTIDTLYFDPWIAVFEEGKIRQIIYVHTPESIRRMAEAYKSFYDWAYEER
                     PIKLKELMPEGQPIRGVTSAKKWMEILAEWQEETGGI"
     gene            3273..3956
                     /gene="thyX"
                     /locus_tag="ENO07_04365"
     CDS             3273..3956
                     /gene="thyX"
                     /locus_tag="ENO07_04365"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HDS01234.1"
                     /translation="MEELKENVMEKMAKEALPEVVLMAITPDGEDVIERACRTCYLSF
                     HRYNPPSSTEDLIKKVIRKGHHSVLEHAAATFRIKGGSRTFTHEFVRHRLASPSQESQ
                     RYVEYGVRKQFEVVVPESMKKHGFSEKYLQLAKETHELYEQMLEAGVPKEDARYIFPG
                     GITSEIVMTANFREWRHICKIRCHERAHWEIRRICLEILKILKKEAPIVFWDFEIDEE
                     NCTARQVSS"
     gene            3971..>4227
                     /locus_tag="ENO07_04370"
     CDS             3971..>4227
                     /locus_tag="ENO07_04370"
                     /inference="COORDINATES: protein motif:HMM:PF00317.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01235.1"
                     /translation="MHIDRQLGLTENAMKVLERRYLIKDDEGKVVESPKDLFTRVARH
                     IAKAEYKYGLDDADVKRTEERYYNAIANREFMPNSPTLMNAG"
BASE COUNT         1212 a          929 c          964 g         1122 t
ORIGIN      
        1 attattgaca aatatcaatc agcaaattaa attattttcg attgaaaaaa ccatcaatat
       61 gacatttgtg catccggggg caatggctgc agagggaaaa tattttattg ctgcacgtga
      121 cccaggggca ttttatcggg ggagggaaac atgtccgcca tatcgttttt caggtcagtg
      181 ctgcttctga cttccttatt atttttcaca ttcggatatc tatcggctca ggagtggggc
      241 aaggtcgagg gctgggaatg ggctctcgag ccgccgccgg agatccccga tgaacccgcc
      301 ctgatcatct tcgacagagg cgatatgaag gtggaccttg aaggcataac attgatgaga
      361 catgtgcgca tcaaggtatt tgacaaagac aatattgatg atgtggccaa tatcgacata
      421 agttaccatg gcgatcagaa gttcagcaaa ttcaaagctc atacaatttt acccaatggc
      481 gacaagaata agatcaagga tgaatttgaa aaaaaagctg ggaatctaaa gacagtggca
      541 tttacatttc cgtctataat caatggttgt attttggaat atcaatacca gatcttccac
      601 gaaaatttcg ggtttctcga gccgtggtat tttcaaaatg atatttacac gttaaaatct
      661 atcttctctg tcacgctata tcctggtttt gagtatgcta cctggcccat taatatccct
      721 gctcatcagc agcaatcgag caggttacat gtgcatgaat tcgggaggtt aacaactagg
      781 tatacgtggg agctggacag cctaatacct gtaaaggccg aaccttttgc cggagcggtc
      841 agcaattata ccgctgcttt gcattttcag ataagatcct tcaagggcac tggtataaga
      901 tttgactttc ctacaaaatg gaaagagatt ggcatagagt taaacaagat aattgatgaa
      961 ttcatagatg atgaggatga gttagaggaa ctttccggga aattgacagc aggcgcgctc
     1021 aacgaagatg aaaagatcgc tcatatttat gattatgtgc gggattcgat tttgacaagg
     1081 ggctctggca tgcttgattt ttcggaggat aagttctcca atttgatgga taatagatac
     1141 ggttcagcta cggccaagaa ccttctccta acagccctgc tgcagaaggc cgggttcaag
     1201 gcccatccgc tcctaattgg aacgagaggt ttttgtaaat tcaacccagc ccatgttcaa
     1261 ctaaatcaat tcaatcatct agtctgctat gttaatctta agataaggga atatttgctc
     1321 gatgcaggta acagagctgt cgctttccca tatgtccctg cctccgatcg catacgcagc
     1381 ggcctgctta tcgatgccga ggcatcccgc ccgatatcgc ttctgccgcc cccgaaacgc
     1441 aaaagcagca cccaattgaa agcgaatatc aatctgcggg aggatggttc ggccgcttgt
     1501 tcaactttca tctgcgttca ggggtatgaa aatgcccggt atgatgatta tttgctggat
     1561 actatttcac agcagcagat cgccacagat attttggatg aatttgagct gaattatgac
     1621 gtggaagagg cggtttcccg ctatttccct gagggggaca gcctcagtat taaattggtg
     1681 atagaaattc cggaatttgc atccgctttg ggagatcaca tgcttatttc tccctttctt
     1741 ataccattgg tcaataatcc attcaaaagc gaggagaggc agtatccgat cgatttccag
     1801 tatgaattca cccgatatca tgagataaca ataaatctgc ctgaaaatat gatagtcggg
     1861 gatcttcccg ataagattaa tagaaacacc gattgtggaa aatataccag gtcttttctg
     1921 acaactggca attcaattgc ggctatcttg tacttcaaac tcaataaaga gacattcctc
     1981 ccgagcgaat atcctggcgt aaaagaaatg ttcgatgaaa ttaccgagac cagcaaggat
     2041 gaaatagccg tgatagtgca agaatgatta tatcccgcct gtctcctcct gccattcggc
     2101 cagtatctcc atccacttct tggcgctggt gaccccgcgg atcggctgcc cctccggcat
     2161 aagctccttc agcttgatgg gacgttcttc gtacgcccaa tcatagaagg acttgtaggc
     2221 ctcggccatt ctgcggatgc tctccggggt atggacatag atgatctgcc tgatcttgcc
     2281 ctcctcaaat acggcaatcc agggatcgaa atagagggta tcgatggtga aggctttgaa
     2341 ccagtcattg gtttcagttt tggtgcagaa aacagtatcc cccctggttt ctttgacagt
     2401 gaaagagact ttggtattca cgatcgaatc ccattccgcc aggggacgaa gttcggtttc
     2461 tgtcagcgga ttggaaaccc gccccatctc gacagtaacc ggctcggcat aaaaggccag
     2521 cacagcatca acatcgtggc tgttgatggc ctcctcgtag gccttcattt ttccttccag
     2581 ccctttcccg caggacataa caagcgccag cagaaaaata actgataatg cagcgaaaac
     2641 agttctattc ttttttctca cttccaatgc tttcctcctg tcggattgcc tgttgcttcc
     2701 tgtttatata aacaatcccg agcggcaatg caatcccata agctccccac attaaaaatc
     2761 tccagggatt catcagatca ggacttaaat cggcagaagt aaacattgat ttaatgggaa
     2821 gaagagtgcc ggaaatagta acccattgtt ttccaaaccc ttagaattag ccaatccacg
     2881 cacatagaag cccaaaactg catcttttaa tccatcaaca ctgcaattcc atccaggtca
     2941 acagcttaaa aattaagcca aaaaactcga ttttcctgca aggcaaaaat acaatataat
     3001 gtggtctata atcggttgat atacaatata ttgtattttt tttaacattt ttttaatatt
     3061 ttgcttgact caatatgatt tagattttat tttaagcacc gcgagtactt ggttaaagta
     3121 tctctttaag taaaagctat aaacttactt agaatctcaa aataattata aaaaagtgac
     3181 cccctctgag ggcttttttt cgcaaaattg caagttacaa tatcaaaatg atttacaggg
     3241 gatcaaaaaa atagaagatt ggattgagtg tcatggaaga attgaaagag aatgttatgg
     3301 aaaaaatggc taaagaagcg ctgcctgagg tagttctgat ggcaattact cctgacggtg
     3361 aggatgtgat cgagcgcgcc tgccgcacat gctacctctc ctttcacagg tataacccgc
     3421 cctcatcgac tgaggacctg atcaagaagg taattcgtaa aggtcatcat tctgtcctgg
     3481 aacatgctgc cgcaacattt cgtatcaagg gtggatcgag aacatttaca cacgaattcg
     3541 tgcgccaccg tctggcctca ccatcacagg aatcgcagcg ttatgtcgag tatggcgtgc
     3601 gcaagcagtt cgaggttgtg gtcccggaga gcatgaagaa acacggcttc tccgaaaaat
     3661 atctgcagct tgccaaagag actcatgagc tctatgagca gatgctggag gcgggtgttc
     3721 ccaaggagga tgcacgctat atatttccgg gcggaatcac ctcggagata gtgatgactg
     3781 cgaatttccg tgaatggcgc cacatctgca aaatcagatg tcatgagcgc gcgcactggg
     3841 aaatccgtag aatttgtctg gaaattctga aaattttgaa aaaggaagcc ccgatagttt
     3901 tttgggattt tgaaatagat gaggaaaact gcacagcacg gcaggtttcc tcctaaggag
     3961 gagggaaatt atgcatatcg acagacagct gggtctgacc gaaaatgcga tgaaggtatt
     4021 ggaacggcga taccttatca aggatgatga agggaaagtt gttgaaagcc cgaaggacct
     4081 gtttacccgg gtggcccggc acattgccaa ggcggagtac aaatacggtc tggatgatgc
     4141 cgatgtcaaa cgtaccgagg agcgttatta caatgctatc gccaaccgcg aattcatgcc
     4201 caattcgccg accctgatga acgccgg
//
LOCUS       DSEB01000244            4224 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001945, whole genome shotgun sequence.
ACCESSION   DSEB01000244 DSEB01000000
VERSION     DSEB01000244.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001945"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            116..1426
                     /locus_tag="ENO07_04375"
     CDS             116..1426
                     /locus_tag="ENO07_04375"
                     /inference="COORDINATES: protein motif:HMM:PF07690.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDS01236.1"
                     /translation="MGIGFRQHPTGFWFIFWGELAERASFYGMKALLVLYMIDKLGFS
                     DGDSSQIASLFTALCYILPIVGGYIADRWLGKFKTIIYFALPYIMGHLILGSFETRAG
                     LFVALVFLAGGSGAIKPNISTLMGLMYEREGKKKLMSQAFSWFYMAINIGAASTTISL
                     PFIRDHYGYSAAFMAPTILMVVSLGIFYLGKKHYPVENVKQVREEHVSVEQKKIDRNI
                     VWRIVGLFSLIVFFWSIFDQAYSTWTLFARDYMILETPFGFRIPPDAIQGLNPVLIVI
                     MTPFFAWLWSKTDKSETVRLSSPKKMLIGFVLVIFCMGVMTVAGFIAVERKISILWEV
                     FAYIMITAGELCISVVGLQLAYEEAPSHMKSRITGLWLATVTLGNVFAGFFARIYTDV
                     LNPGEYFGLMTILVVVVTIGFYFVGRKFEQKPERQEKATSPPRT"
     gene            complement(1442..2878)
                     /locus_tag="ENO07_04380"
     CDS             complement(1442..2878)
                     /locus_tag="ENO07_04380"
                     /inference="COORDINATES: protein motif:HMM:PF08668.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HDS01237.1"
                     /translation="MIRLILSNGKLVSATVFQDCKLNHRNPNNLLTSACFCRYLNGAT
                     MEEKQVLKRVARLTQLATLPEVLSQILKIADDPESPLDDLAKVILKDISLTARILSVA
                     NSSRHGRDSEVSSIRDAIMVLGSRTVKAIALSVSVVNVINRLDTQVNLQTYWKHSLEV
                     AMISELIASRVGLKEKEEAYIAGIFHDIGILSLDACFPREYARVWKDAQKGGSILDLE
                     ESIFGTNHCRVGSYLLSKWNLPEIFSHAISRHHDAFKSEEPVNDQIIPQIINLAEGLA
                     KYRLDINSPLEDIELANRKVVMKNLGLDNADLSEIEAEIIPKFHDAASCLEIEVGSPM
                     ELLTEANSRLFEVYHEMQEMLDSFKKNANFKDHIEYDKLAVEILHTVVATFSHYFNNA
                     CASMLGRAQLIEIALKKGELEDNRDILHNSLIAIHNGVNNITGTISELKQVKSFKTTL
                     YHDKTLIIDLEDSLKKYKPQKAKMKARV"
     gene            complement(2793..4025)
                     /locus_tag="ENO07_04385"
     CDS             complement(2793..4025)
                     /locus_tag="ENO07_04385"
                     /inference="COORDINATES: protein motif:HMM:PF13482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01238.1"
                     /translation="MGIEEQFRRFEKFIKTDLPAQKSLHLDKISQDLSAGIRETPEGN
                     FLVKETIYGPGDLHGQFSFSDLDLSHPLRFDSFSCPPDEGTFNLKNIVVIDTETTGLS
                     GGVGTVPFLVGLGFFRDGTYVVRQHFLPDYSEEHGFYKYMFDDLPGNMIVSFNGKAFD
                     LPLLTNRFLIQRTGSEFFSRKHLDVLFTLRRFFRLKLPDCTLKTAERALLEFERLNDI
                     PSEQIPQTYFNYLQTGETSEIYQVIKHNIWDIVSTMGVMVELERYVESAEKMDKLSDV
                     DSWAIGKFYLQRKDYDRAFSSFSRSEGTEQEFHFKNLFQASMISKKLCDFETAVSYWK
                     MLSESFNPHYFAALEEMAKLFEHRSREFDKAISCCRRALDSIYQVSQLHDQADFEQWQ
                     TRFSHRLSRLQTKSSKSK"
BASE COUNT         1048 a          991 c         1005 g         1180 t
ORIGIN      
        1 actaatacca gttcgacaat atgattataa aaatcattgt ctctgaggat atttattatt
       61 ataatatcct atccaaatgc gcaatggtat ttgcacatgc agtgagggat aattagtggg
      121 aattggattc agacaacacc cgaccggatt ctggttcata ttttggggcg aactggcaga
      181 gcgtgccagc ttctatggca tgaaagccct gctcgtttta tacatgatcg ataaacttgg
      241 tttctccgac ggggatagct cgcagatagc ctccttattc actgcacttt gctacattct
      301 tccaatcgtg ggcggataca ttgccgatag atggctggga aaatttaaaa ctataattta
      361 cttcgcactt ccctatataa tggggcatct tattctggga agtttcgaaa cccgcgccgg
      421 tctctttgtt gccctcgtat ttcttgccgg cggatcgggc gcgatcaagc ccaatatcag
      481 caccctgatg ggtctgatgt atgaacggga aggcaagaag aaattgatgt ctcaagcctt
      541 ctcatggttc tacatggcca tcaatatcgg tgccgcatcg accacaatct cgctgccatt
      601 tatacgggac cattatggct attcggctgc ctttatggcg cccacgatcc ttatggtcgt
      661 ctctctgggt attttctatc tcggcaagaa gcattatccc gtggagaatg taaagcaggt
      721 ccgcgaggaa catgtctcgg tcgaacagaa aaaaatcgac cgaaatatag tctggaggat
      781 tgtcgggctg ttttcactga tcgtattctt ctggtcaatt ttcgatcagg catactcaac
      841 ctggacattg tttgcacgcg attacatgat tctggaaaca ccgttcggct tcaggatacc
      901 gcctgatgcg attcaggggc tgaatcctgt actgatcgtc atcatgacac ctttctttgc
      961 atggctctgg tcgaaaactg acaagagcga gacagtacga ttatcctcgc ccaagaagat
     1021 gctgatcgga tttgttctgg tgatcttctg tatgggcgta atgactgtgg cgggctttat
     1081 tgccgtggag cgcaagatat ctattctatg ggaggttttc gcctatataa tgattaccgc
     1141 cggagaattg tgtatctctg tcgtgggcct gcaactggcc tatgaagagg ctcccagcca
     1201 catgaaatcc cgaatcaccg gtctctggct ggcgacagta accctgggaa atgtcttcgc
     1261 cggatttttc gcacgtattt ataccgatgt gttgaatccg ggcgagtatt ttgggctgat
     1321 gaccatttta gtggttgttg tgactattgg attttacttt gtgggcagaa aattcgagca
     1381 gaaaccggag cgccaggaaa aggccacctc tcccccgcgg acatagcagg tgaatttaga
     1441 attacacccg cgccttcatc ttcgcttttt ggggcttgta cttcttgaga gagtcttcca
     1501 gatcaattat cagagttttg tcgtgataga gcgtggtctt aaaagatttg acctgtttca
     1561 gttctgagat agtcccggtg atgttgttta caccattgtg aatggcaatc aaggagttat
     1621 gcaggatgtc tctgttgtcc tccagttccc cttttttgag agcgatttca atcaactggg
     1681 cacgtcccag catcgaggcg caggcattgt tgaaatagtg cgagaatgtc gccacgacgg
     1741 tatgcaatat ctcgaccgcc aatttatcgt attcgatatg atccttgaaa ttagcattct
     1801 tcttgaaact atccagcatt tcctgcatct cgtggtacac ctcaaataac ctggaattcg
     1861 cctcggtcag aagctccatg ggagaaccga cttctatttc caggcaactg gcggcatcat
     1921 ggaattttgg aatgatctcc gcttcaatct ctgacaggtc ggcgttatcg aggccgagat
     1981 tcttcatcac cacttttcta ttcgctaatt cgatatcctc gagaggagaa ttaatatcca
     2041 gtctatattt tgccagcccc tccgccaggt ttattatctg cggtataatc tgatcattta
     2101 caggctcttc cgacttaaaa gcatcatggt gcctgcttat ggcatgcgag aagatttcag
     2161 gcagattcca tttagagaga agatatgatc ccacccggca atgattggtc ccgaagattg
     2221 actcctccag gtcgaggatt gagccgccct tctgagcatc tttccagaca cgcgcatatt
     2281 cacgcgggaa acaggcatcc aacgagagaa tgccgatatc atgaaaaatg ccggcaatat
     2341 aggcctcttc cttctctttc aagccgaccc tggaggcaat taattcactg atcatggcaa
     2401 cttcaagaga atgcttccag tatgtctgga ggtttacctg cgtatcgaga cggttgatta
     2461 cattcacaac ggagaccgag agcgcgatgg ccttaacagt acgtgatccc aggaccatga
     2521 tggcatcgcg tatggaagag acctcagaat ccctgccatg acgagacgaa ttggccacgc
     2581 tcagaatcct ggcggtcagt gatatatcct tgaggataac cttggcgaga tcatcaagag
     2641 gcgattcggg atcatccgct atcttcaaaa tttgcgaaag cacctccggc agtgtggcca
     2701 gctgtgttaa ccgtgctact ctttttaata cctgtttctc ttccattgtt gctccattta
     2761 agtatcggca aaaacaggcg ctagttaaaa gattatttgg atttcgatga tttagtttgc
     2821 agtcttgaaa gacggtggct gaaacgagtt tgccattgct caaaatcagc ctgatcatgg
     2881 agctgggaga cctggtatat tgaatcgagg gctcgacggc agcatgagat ggctttatca
     2941 aattccctgg agcggtgttc gaagagcttg gccatttcct ccagcgcggc aaaataatgg
     3001 ggattgaatg actccgaaag catcttccag tagctgacgg ctgtttcaaa atcgcacagt
     3061 ttcttggaaa tcattgaggc ttgaaacagg tttttaaagt gaaactcctg ctcggtaccc
     3121 tccgatcggg agaaacttga aaacgccctg tcgtaatcct tgcgctgaag atagaatttg
     3181 cctatggccc agctgtccac gtcggataat ttatccattt tctcggcgct ttcaacatag
     3241 cgttccagct cgaccattac gcccattgtg gatacaatat cccatatatt gtgcttaata
     3301 acctgataga tctccgaggt ctcgccggtc tggagataat tgaaataggt ctgcggaatc
     3361 tgctcggagg gaatatcatt taagcgctca aattccagaa gggctctttc tgcggtcttc
     3421 aaggtgcagt cgggcagttt caacctgaag aagcggcgca aggtaaacaa cacatccaga
     3481 tgcttcctgg agaagaattc cgatccggtg cgctgtatca aaaagcggtt tgtcaggagc
     3541 ggaaggtcga acgccttgcc gttaaaggag acaatcatat tacccggaag atcatcaaac
     3601 atatatttat aaaaaccgtg ctcctcgctg taatcgggga gaaaatgctg cctgacaacg
     3661 taagtgccgt cacggaagaa gcccagtccg accagaaacg gcacagtacc cacgcccccg
     3721 gagagaccgg ttgtctctgt gtcgattacg actatatttt tcaggttgaa tgtgccctca
     3781 tccggcggac aggagaagct gtcgaatctg agcggatggc tgaggtccag atcagaaaat
     3841 gaaaactggc cgtgaaggtc tccgggccca taaatcgttt ccttgaccag gaaatttccc
     3901 tccggtgttt ctcttattcc ggcagacaaa tcctgcgata ttttatcaag atgcagggat
     3961 ttctgcgccg ggagatcggt cttgatgaac ttctcgaaac ggcggaattg ttcttctatt
     4021 cccatctatt tgcttttctc ggcagctatt ctgcgagctt tctcgaaact cgagagcacg
     4081 aatgaatctt tttcatcttt actatattta cgaacaagtt ccccggtttt ttcaggcttt
     4141 atttttgcga ttgcggtaat tgcaaacccg cgcgcccggt tatccggtga attcaataaa
     4201 tctttcagcc tgtcgaatgc atcc
//
LOCUS       DSEB01000245            5710 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000981, whole genome shotgun sequence.
ACCESSION   DSEB01000245 DSEB01000000
VERSION     DSEB01000245.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5710)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5710
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000981"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            224..1342
                     /locus_tag="ENO07_04390"
     CDS             224..1342
                     /locus_tag="ENO07_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01239.1"
                     /translation="MNTGRSYVNVFRGLQTIGENSRIGFLASDRRFEHDGSGTVLALD
                     FDIRLAQNYRIDGQYIGTHTREPDDSSFDSMLGDLKFDSDKYTAAFDGESYYGTAFIT
                     RLLRRSRSFNVMLDYNQISPTYRTQLGYDPINSHRTFSAGAEYVFYPKNSIFQRITPS
                     AFQFRRWEFEGGDLRNSSSNISLNMQTRFAQTYFNIYYNRDWQRYRDIEFDRLWSIGL
                     DMGSRLSNRLGYNLSLRRGVGVSYYDLARGRETGAFFGLDLKPLDRLIIEPYINYSRS
                     EHKETGEVLFDGYIFRTRLRYQANREMSIRLVLQYNDFRERWDIDPLLTYRLGSFTVF
                     YVGSTYDYDNIEYDPGKVRQWKLSSRQFFMKLQYLFRS"
     gene            complement(1447..2319)
                     /locus_tag="ENO07_04395"
     CDS             complement(1447..2319)
                     /locus_tag="ENO07_04395"
                     /inference="COORDINATES: protein motif:HMM:PF13557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HDS01240.1"
                     /translation="MHKIYQITAIALFTAFLLFTELFSFQSASAAGISIDAGLTPAEN
                     RWIFRSQVRYMQRDNDQLGSQREMKAYMFPVVIAYGLRSDLTVMARQAFIRREMSMMG
                     QSTTNSGLGDLLMLSKYRLIRINTPGYTLGVAPTLGLEFPTGKDEFTSSSYDLHFGSF
                     FSGRLRSLGMDLNITYIWNGMAETGGAAVDPGNEFSLQAAFAYQVGIGSYSNFALAPV
                     LESSYLKISADSEDGETLADTGESILWLSPGFKITWSSFIFESLVQFPVWQDQEGLQT
                     ERDPTFLVGIRLMN"
     gene            complement(2323..2658)
                     /locus_tag="ENO07_04400"
     CDS             complement(2323..2658)
                     /locus_tag="ENO07_04400"
                     /inference="COORDINATES: protein motif:HMM:PF00403.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01241.1"
                     /translation="MKNFIIVSALFTASVFLFITMIACGSSQKNISDTSYEESEVRIY
                     EVYGMDCPGCHGGLENLVNKVPGVKSSRANWEKQSLQVVLDSENDVDDQSIYEAIKKA
                     NFTPGERLQ"
     gene            complement(2797..3669)
                     /locus_tag="ENO07_04405"
     CDS             complement(2797..3669)
                     /locus_tag="ENO07_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01242.1"
                     /translation="MTRYARIDDYITQIKDILRGKSNQKAADLRKLIYKSRKILNRHS
                     GKQYSVDQAVEKLLHPGWIKSVARFNPDEWQDIADRMYIRARKILGNVQKPEIILYPS
                     FQRFNGRVYEVDRTPYIAASPDFPHTTGKNLQVLMAHEYAHFARWRKTGIPSENVPIY
                     ACIFEEGWATWMSIKLLPEYTLTHLFMSNLHKSIHMPDPKGGYLRWCKKHLKSIASEA
                     AKSLNSKDHKALGRFFQCQRFGNDNTPIRTGYYLGYRMIDMLAGQMPARKLMLEKPTR
                     RKVSGWLDQLLDQP"
     gene            complement(3766..5121)
                     /locus_tag="ENO07_04410"
     CDS             complement(3766..5121)
                     /locus_tag="ENO07_04410"
                     /inference="COORDINATES: protein motif:HMM:PF05576.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDS01243.1"
                     /translation="MKRIFLLLGLLALILSCSDKTSGPEPQDLLQRLQALPDLEVVEI
                     TPPYGYNRAFELTITQPLDHQNPGGQTFEQKFYISHLDESGPMVMHISGYAIGSNYAF
                     EMARILDANQIYVGHRFFSGARPITLEWQYLTLEQAAADHHRIAELLKPIYDGIWVNT
                     GVSKGGMTALFHRRFYPDDVDATVAYVAPLPLSIDDARFDNFLENEVGDEACRQKIKS
                     FQRMALENKAALYPYLEDYAQESGYAFNMDFNLILEYLVLEYPFAFWQYGDGDCAIVP
                     DQGAPVSAIWNHLNYVVSMNYFSEELISYFEPHYYQAFTQFGYYRLITDHLEDLLTEA
                     DNYSYSILAPQDVQLVFDASIMPDIVSWLQNSGDNTIYIYGGQDPWTAGAIDLNGTAE
                     ALKIIEPGGNHGINIAQLTQRDLVYTTLENWLGIEINPETPAFAQPQPSIKELPPFYR
                     K"
BASE COUNT         1487 a         1395 c         1352 g         1476 t
ORIGIN      
        1 caatactaca tttgccctat tttatcccga gcggcggccc tttttccagg agggcagcga
       61 cctttttcgc acgctttaca attcttttta tacccgcacg atcaatgacc cgaaatttgc
      121 agcaaaaatg attggacgtc tgggcagaac aaatatttcg gcattatccg cagttgatga
      181 aaattcccct tatataatcc cgctccggga aagcagcatt cttatgaata ccggccgaag
      241 ttatgtcaat gtttttcggg gcctgcagac aatcggtgag aattcacgta tcggctttct
      301 ggcttccgac cggcgctttg agcatgacgg ttccgggact gtgctggcgc tggactttga
      361 catcaggctt gcgcagaact accggattga cggacagtat atcggcaccc atacccgcga
      421 gcccgatgac agttcatttg actcgatgct cggcgatttg aaattcgata gcgataaata
      481 tacggcggcc tttgatggcg aatcctacta tggaacagca tttataacca gactgttgcg
      541 gagatcccgc agtttcaatg ttatgctgga ttacaaccag ataagtccaa cctacaggac
      601 ccagctcggt tacgatccga taaacagcca tcggacattt tcagcaggcg ccgaatatgt
      661 cttttacccc aaaaactcaa tattccagag aatcacacct tcggcatttc aattcaggcg
      721 atgggaattc gagggcggtg atctgaggaa cagcagctct aatatcagcc tcaatatgca
      781 gaccagattt gcccagactt atttcaatat atattacaat agagactggc agagatatag
      841 agatattgaa ttcgacaggc tctggtcaat tggtctcgat atgggaagcc gtttgagcaa
      901 caggctggga tataatctat cactgcggcg cggcgtcggg gtttcctatt acgatcttgc
      961 caggggacgc gagacaggag cattcttcgg cctggatctg aagccgctcg accgcctgat
     1021 aatagagccg tatataaatt actcccgaag cgaacacaag gagaccgggg aggtcttatt
     1081 tgacggctat atcttcagga caagattacg ataccaggcc aaccgtgaaa tgtccataag
     1141 acttgtgctg cagtataatg acttcaggga acgatgggat atagatcctt tgctgaccta
     1201 caggctgggc tccttcactg tcttctatgt cggttcaaca tacgactatg acaatatcga
     1261 atatgatccg ggcaaggtca ggcagtggaa gctgagttca cgtcaattct ttatgaagct
     1321 gcagtacctg ttccggtcat gatggtgaat atgaaataat ttgaagacgg gaattcactt
     1381 taatctactc gggaccggtg agatagactc ttgccggtct tcttattcag gaagcagtat
     1441 gatggcctaa ttcataagcc tgatgcccac aagaaaagtc gggtcacgtt cggtttgcag
     1501 accttcctga tcctgccaga ccggaaactg caccaggctt tcgaaaatga aagatgacca
     1561 ggtgatcttg aaaccgggcg acagccagag gatggattcg cctgtatctg ccagagtctc
     1621 cccatcttcg gaatccgccg aaatcttaag gtaggatgat tccagaaccg gggccagggc
     1681 aaaattagaa tacgatccaa ttcccacctg ataagcgaaa gctgcctgta gcgagaattc
     1741 attccccgga tcaaccgcgg cgccgcctgt ttctgccatg ccattccaga tatatgtaat
     1801 attcaggtcc atacccaggg agcgcagccg cccggagaaa aagctgccga agtgcagatc
     1861 gtaactactt gatgtgaact catctttgcc cgttggaaat tccagaccaa gtgttggtgc
     1921 aactcccaga gtatatccgg gagtatttat ccggatcagc ctgtacttgc tgagcataag
     1981 caaatccccc aggcccgaat tagttgtact ctgccccatc attgacattt cacgccggat
     2041 aaaggcctgc cgcgccatca ctgtcagatc cgatctcagt ccgtaggcga taaccacagg
     2101 aaacatgtaa gccttcatct ctctttgaga tccgagttga tcattgtccc tctgcatata
     2161 gcgcacctgt gaccgaaaaa tccagcggtt ttcggcggga gtcagaccgg catcaatgct
     2221 gataccggca gcggaagcgc tttgaaagct gaataattct gtaaatagca gaaatgcggt
     2281 aaagagcgct attgcagtta tttgataaat tttatgcata ggctattgca aacgctctcc
     2341 cggggtgaaa ttcgctttct tgatagcctc gtaaatagac tgatcatcta cgtcattttc
     2401 agaatcaagt acaacctgca agctctgctt ctcccaattc gcccttgagc ttttcactcc
     2461 ggggaccttg ttgacgagat tctccaaacc tccatggcat ccggggcagt ccatgccgta
     2521 gacttcgtat atcctgactt cactttcttc gtaacttgta tcgctaatat tcttttggct
     2581 ggagccgcag gcaatcatgg ttatgaataa aaaaacagag gcggtaaaca gtgctgaaac
     2641 tattatgaaa tttttcataa gaagctgtcc ctcagattac aattctgtaa catatacaaa
     2701 attagtccgg tatagtttta gaaacaattc aaatatccgg aggttccgcc tggcaattgc
     2761 actttgcgta tttcaataat tggcagctgc ccgttcttat ggttgatcca gcaactgatc
     2821 cagccagccg gagacttttc tacgggtagg tttttcaagc attaatttcc gagcgggcat
     2881 ctgtccggcc agcatatcga tcatcctgta gccgagataa tagccggttc tgatgggagt
     2941 attatcattc ccaaaacgct ggcactggaa gaatcgccct aaggccttat gatcctttga
     3001 attcagcgat tttgccgcct cgcttgcaat agatttcaga tgctttttgc accagcgcaa
     3061 gtatccgcct tttggatcgg gcatatggat ggacttatgc agattcgaca taaataaatg
     3121 ggtcaatgta tactctggca acagcttaat agacatccag gtagcccagc cttcttcaaa
     3181 gatgcaggca tagataggca cattttccga aggtattccg gtttttcgcc agcgggcaaa
     3241 atgcgcatat tcatgagcca taagaacttg caggtttttg cctgtggtgt gaggaaaatc
     3301 cggagatgcg gctatgtatg gcgtgcggtc aacttcatat accctcccat tgaacctctg
     3361 aaagctgggg tacagaatta tttccggctt ctgtacattt ccaagaatct ttcgtgcgcg
     3421 aatatacatc cgatcggcaa tatcctgcca ttcatcagga ttgaaccggg ccacgctttt
     3481 gatccagccc ggatgaagga gtttctcaac agcctgatca acagaatact gcttgccgga
     3541 atggcgatta agtatttttc gggacttgta aatcagcttc ctgaggtcag ccgctttttg
     3601 atttgatttt ccccgcagga tatcctttat ctgtgtgata taatcgtcga ttctggcata
     3661 tctggtcatg tcttctcctg atcgtggtca aagatatata caatacattg cctgctacat
     3721 tatgtcaaca tgtcagagcg aagataaata aaatcccaat cacgattatt ttctatagaa
     3781 cgggggaagt tcttttatgc tcggctgtgg ttgagcgaaa gccggagtct ccggattgat
     3841 ttctatgccc agccagtttt ccagagtggt ataaaccaga tcacgctggg taagttgagc
     3901 gatgttgatg ccgtgatttc cgcccggttc tatgattttc aaagcctcgg cagttccatt
     3961 taggtcaatt gcgcctgcgg tccatggatc ctggccgcca tagatataga ttgtattatc
     4021 tccgctgttt tggagccagg agactatatc gggcataata gaagcatcaa atacaagttg
     4081 gacatcctgc ggagccaata tactgtagct gtagttatcc gcctccgtca ggagatcctc
     4141 gagatgatcg gttatcaggc ggtaataccc aaattgcgta aaggcctgat aataatgcgg
     4201 ctcgaaatag ctgatcagct cttcggaaaa ataattcatg ctgacgacat agttcagatg
     4261 attccagatg gctgagaccg gggcgccctg atccgggaca atggcgcagt cgccatcgcc
     4321 gtactgccag aaagcaaacg gatactcgag caccagatat tccaggatca gattaaaatc
     4381 catgttaaac gcatacccgg attcctgagc ataatcctcc aaataaggat acaatgcggc
     4441 cttattctcc agagccatcc tctggaacga tttgattttc tgccggcagg cctcatcccc
     4501 gacttcattt tcgaggaaat tatcgaaccg ggcatcatca attgacaacg gcagaggggc
     4561 gacatatgcc acggtagcat caacgtcatc cggatagaat ctgcgatgaa acaacgctgt
     4621 cataccgccc ttgctgactc cggtattgac ccagatgccg tcatatatcg gttttaaaag
     4681 ctccgctatg cggtgatggt cggcggcagc ctgctctaat gtcaggtatt gccattcgag
     4741 cgttattggt ctggcgccgg aaaaaaaccg gtggccgaca taaatctggt tggcatccag
     4801 aatgcgcgcc atctcaaaag cataattgct gccgatggca taaccggaaa tatgcatgac
     4861 catcggcccg gattcatcaa ggtgcgagat gtaaaacttc tgttcaaaag tctggccccc
     4921 gggattctga tgatcaagag gctgggtgat ggtcagctcg aaagcccgat tgtatccata
     4981 gggtggtgtt atctcgacaa cttcgagatc cggaagagcc tgcagtcgct gcagaagatc
     5041 ctgcggttcg ggaccggagg ttttgtcact gcaggataat atcagggcca gaaggcccaa
     5101 taacaggaaa attcttttca tatttgaagc acctttttct tttatctgta atacggccaa
     5161 gaatgaatgt tgtttaaaga caagccagag taatacacgc ccaaactaat cccgaacatg
     5221 cagtccagcg ccattgccgg tatttcattc tgtatcatag cccacgccca gctcgacttt
     5281 gggtaatggc ttctcagcat ttgtgccggg ctggatccag aggttgttgt tgcgggcgaa
     5341 gtcgtattcc tcgggataga gatcattccc gcccgtatca agaaacagcc ccaggcagat
     5401 gatatgatcc cgcaggctgc cccggctggc aacattggcg cgtcccagag tggtggcagc
     5461 ggtaacataa taagcgtcat ccccggattt gtcccagaag atgccgaaac cattggcatt
     5521 cccgccgccc aacgatagat tgggggcatt atagatatca ttgccgcttt cttcgatgaa
     5581 gatgcccagg gtgaaatcat gcccagcgcc aatagccata ttcatctcag ccgtataacg
     5641 gtcgtctccg gccgattcaa aaaggatgcc caagccgaaa tgcgctcccg agccctgcac
     5701 ataccagacg
//
LOCUS       DSEB01000246            4010 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002168, whole genome shotgun sequence.
ACCESSION   DSEB01000246 DSEB01000000
VERSION     DSEB01000246.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002168"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1143)
                     /locus_tag="ENO07_04415"
     CDS             complement(<1..1143)
                     /locus_tag="ENO07_04415"
                     /inference="COORDINATES: protein motif:HMM:PF13231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HDS01244.1"
                     /translation="MDTKQISARQELYFLLAVTAIHILFRVVYIWQYQGSEYWGHLTV
                     DARFHYLWAQAIASGDFWGSQVFFRAPFYAYFLALLQLIFSDSILGIVIAQNIIGIIS
                     FLLTYKLCKEIFGARFAQIAAILYIFTFNFYFFESELLLDFLLLFFLPLFFLTIMRAE
                     KSHGLLFWLLAGVILGLATATRPTVLILAVIVPLFFLRPSSKLFNFKSWIKRSALFLA
                     GLIIILLPIAIRNQVVGGQFTALPTQGGINFYIGNNQQADGWSAAMPDSLGGAFWQYA
                     ECKQIAERETGRELNPSEVSRFWYRKGLEEMADKPGRALQLYLKKITLLFTNDNISNN
                     RNIPQFKNSISVSAILQISWWLLFPFGLVGMIAGLKRNYKARLMALF"
     gene            1420..>4010
                     /locus_tag="ENO07_04420"
     CDS             1420..>4010
                     /locus_tag="ENO07_04420"
                     /inference="COORDINATES: protein motif:HMM:PF00082.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01245.1"
                     /translation="MERLKENAMNSKHSTEITKIKFLLALLPLILLFAPTREIQCGEL
                     SSSLHGISPAMNDARIQVLVFLEDDGVSILSKSSSNYNHSRSQLHKELFTELKSRSNA
                     NINRFEGRLRDSDLPADVIRTFWITSAALIDVPVAELDRLAGLEGVEFVAPDTTLALI
                     DPVSISMSSEFSEGASEHLYSIGADKLWRRGLTGKGRIVGSFDTGVEGSHPALNSAWR
                     GNSVDGYSSAWFDPYGSTFPFDDNGHGTHTMGIMVGLDGSDTIGIAFNAEWISAGVID
                     RGSGLSQTISDILAAFEWAADPDGDPNTISDVPDVICHSWGIPQSIFPPCDNTFWSAI
                     DNLESLGIVCVFAAGNEGPDSMSLRVPADRASGPLNAFSVGAIDQTDPNFVVAAFSSR
                     GPSSCDNSRTKPEVVAPGVSIRSSYKGGTYRTISGTSMAAPIVAGAVALLREYNPEAT
                     SEQIKQAIFQSAVDLGARGADNSYGNGLIDLEAALALIPPPDSPHIQISSFVAGDGND
                     DIFESSEIVDLTLNVISQYASINSLWARIFIADEYARVLTDSAYFGYLESDQEGNNSF
                     SPFVIQLSEQVRPGGRMPVDIDFYDVNGEVINAGRIEIVVGQSQNAVFHTLSNGSFKL
                     TVDNFGGIGHGENSPSPAGVIGFSPVESEFDILPEFSLMIATGNDARVSDASRSETEF
                     ISDNDFLASREMEAVFQNPGSFGAADLFGYYSDSLATEPLGVSIRQRTSIFDDPELEN
                     CVIIEYSILPENGLNGDLYYLGFLMDWDLADGGGGVEQSAFDVSGDFCYFYNQDDDLY
                     VGLRFLNKPVYGYKILPNIPGGKSLLSESSKYQHLTSGEINGGVEKWTDYFSIISARE
                     TYNGSGD"
BASE COUNT         1014 a          967 c         1009 g         1020 t
ORIGIN      
        1 aaacagagcc atcaatctgg ccttatagtt gcgtttgagc ccggcgatca tacccaccag
       61 cccgaagggg aacagaagcc accaggagat ctgcaaaatc gccgagaccg aaatcgaatt
      121 cttgaattgg ggaatatttc gattattcga gatattatca tttgtgaata gcaatgtgat
      181 tttctttaga taaagctgca aagcccttcc gggtttatcg gccatctctt caagtccctt
      241 tctgtaccag aatcttgaga cctcggatgg gttcagctcc cggccggttt cccgctccgc
      301 tatttgcttg cactcggcat attgccagaa ggctccaccc agtgaatccg gcatagccgc
      361 cgaccatcca tcagcctgct gattattccc gatatagaag tttattccgc cctgggtcgg
      421 aagggcggta aactgtcctc cgacaacctg gtttcgtatt gcaatcggca ggagaattat
      481 gatcagcccc gcaaggaaaa gcgcgcttct tttgatccag cttttaaagt tgaacaattt
      541 tgaggagggt cgcaaaaaaa acaggggtac tattaccgcc agtatcaaaa ctgtgggacg
      601 ggtggcggtt gccagcccca ggatcacgcc cgccagcagc cagaataaga ggccgtgtga
      661 tttttcggcg cgcataattg tcaagaagaa caggggcagg aaaaataaca gcaaaaaatc
      721 cagcagcagc tcagattcga agaaatagaa gttgaaggta aatatataga ggatggccgc
      781 tatttgagcg aacctggcgc caaagatctc tttacagagc ttgtatgtca ggaggaagga
      841 aataattcct atgatgttct gggcgatcac gatccccagt atcgagtccg aaaatataag
      901 ctgcagcagg gcaagaaaat aagcgtaaaa aggggcccgg aagaatacct ggctgcccca
      961 gaaatcgcct gaagcaatcg cctgggccca gaggtaatga aagcgggcgt caacagttaa
     1021 atgaccccaa tattcggaac cctgatactg ccagatgtag acgaccctga aaagaatatg
     1081 aatcgccgta actgcaagca gaaaatagag ttcctgccga gccgagatct gttttgtatc
     1141 catcaaataa tattttaagc tatttaaata tcttgaacaa gtattttcac agatattgca
     1201 aaggtatgca tgcaaagagg ggatgtgaaa tcgctttcat atcgtcaaat ataaaacatt
     1261 tttgcctgcg aataactgag ctctttaatg tgccggattt aagctcttga catcatataa
     1321 cttatatccg taattgtacc ggcagaatag atgaattgga tatgatttcc atggatattg
     1381 ccagggttca tctgcggcat cgcttttgct cgattaggca tggagcgttt aaaggagaat
     1441 gcaatgaatt ccaagcacag cacagagatc acaaaaataa aatttctttt agctctttta
     1501 ccattaattc tcctttttgc tccgaccagg gaaatccaat gcggggagct ttccagctcc
     1561 ctgcatggta tttctcccgc catgaatgac gcaagaatac aggtgctggt gtttcttgag
     1621 gatgatgggg tttcgatttt gtccaaatcc tcttctaatt ataaccatag ccgcagccag
     1681 cttcataaag agctcttcac cgaactaaaa tcgcgctcaa atgccaatat aaatagattc
     1741 gaaggtagac tcagagattc agatctgccg gccgatgtca tccggacctt ctggataaca
     1801 tctgcagccc tgattgatgt tccggtcgct gaacttgaca gactggctgg tcttgagggt
     1861 gtagaatttg tggctccgga tacgactctg gcgctgatcg atccagtctc gatttcgatg
     1921 tcctcggaat tttcggaggg cgcctccgaa catctttact caatcggagc cgataagctc
     1981 tggagacgtg gattgaccgg caagggcaga attgtcggca gtttcgatac cggagtggag
     2041 ggctctcatc cggcgctcaa ctctgcctgg cggggtaact cggttgacgg ttattcctca
     2101 gcctggttcg acccctatgg cagtacgttc ccatttgacg acaatggcca tgggacccac
     2161 actatgggga ttatggtcgg cctggatggg agcgatacaa taggtatagc tttcaatgcc
     2221 gagtggatct cggccggcgt tatcgatcgg ggctcaggcc tttcgcagac catctcggat
     2281 atcctggccg cttttgaatg ggcagccgat ccggatggtg atcccaacac tatcagcgat
     2341 gttccggatg taatctgcca ttcatggggg atcccccaga gtattttccc cccctgtgac
     2401 aatactttct ggtcggcgat cgacaatctt gaaagtctgg ggatagtatg tgtttttgcg
     2461 gccggtaacg agggtccgga ttcaatgtcc ctgcgcgtcc cggccgacag ggccagcgga
     2521 cctttgaatg ccttctccgt tggagctatt gaccaaaccg atccgaactt cgtagtagcg
     2581 gcgttctcca gcaggggacc ctcaagctgc gacaattcca ggaccaaacc ggaagttgtg
     2641 gccccggggg tcagtatccg ttcatcctac aaaggcggaa cttacaggac tatcagcggt
     2701 acctcgatgg ccgcgccgat tgtggccggc gctgtagcgc tattgcgtga atataatccc
     2761 gaggctacct ccgaacagat caaacaggct atttttcagt ccgcagtcga cctcggtgcc
     2821 aggggagccg ataattccta tggcaacggt ttgatagatc tcgaggcagc cctggcattg
     2881 attcctcctc ccgattcgcc tcacatccag atttcgagtt tcgtggctgg cgacgggaat
     2941 gatgatattt ttgagtcaag cgaaatcgtc gacctgacgc ttaatgttat cagccagtat
     3001 gcctcaatca atagtctctg ggccagaatt tttatcgccg atgaatatgc ccgggtgctc
     3061 acagattctg cctatttcgg ttatctggaa tcggaccagg aagggaacaa cagcttcagt
     3121 ccgttcgtaa ttcagctttc cgagcaggtc aggcccggcg gtagaatgcc cgtggatatc
     3181 gatttttacg atgtcaatgg cgaggttatc aatgccggac gcatcgagat tgtagtcggc
     3241 cagagccaga atgctgtatt ccatactttg tccaacggca gtttcaagct taccgtcgat
     3301 aatttcggag gaatcggaca cggcgaaaat tcaccatcgc ctgccggcgt gataggtttc
     3361 agccccgttg aaagtgagtt cgatatactt cccgaattct cgctgatgat tgccaccggg
     3421 aatgatgccc gggtttcaga tgcatcccgc tcggagaccg aatttatctc cgacaatgat
     3481 tttctggcca gccgggaaat ggaagccgtt ttccagaatc ccggttcttt tggtgcggcc
     3541 gatcttttcg gatattacag cgactcgctg gcgaccgaac cgctgggagt ttccatcagg
     3601 cagcgtacat ctatattcga cgatcccgaa cttgaaaatt gcgtaattat cgaatattcc
     3661 attttgcccg aaaatggcct caacggagat ctttattatc tcggattctt gatggattgg
     3721 gacctggctg atggcggagg cggtgtcgaa caatcagcct tcgatgttag cggtgatttc
     3781 tgttatttct ataaccagga tgatgatctt tatgtcggcc tgcgcttcct caacaagcct
     3841 gtttatgggt ataaaattct gcccaatatt ccaggcggaa aatcacttct cagcgaatca
     3901 agcaagtacc agcatcttac ctcgggagag attaatggcg gtgtagagaa gtggactgat
     3961 tatttcagca taatctcggc cagggaaacc tataatggca gcggcgacag
//
LOCUS       DSEB01000247            1981 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1010311, whole genome shotgun sequence.
ACCESSION   DSEB01000247 DSEB01000000
VERSION     DSEB01000247.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1010311"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..553
                     /locus_tag="ENO07_04425"
     CDS             <1..553
                     /locus_tag="ENO07_04425"
                     /inference="COORDINATES: protein motif:HMM:PF08402.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDS01246.1"
                     /translation="RTELKNLQRRLEITTLYVTHDQTEAMTLGDRVAILKEGEIEQIG
                     TPLELYDKPRNIFVARFLGSSPINIFDAEITAEEDKIFIRIAGNRIELPHNRMEEVRG
                     FKADKVIFGIRPEDVEPQSDAEGSPVAGEIQDIELLGRETLLHISVDDNTVRALSESS
                     KWEIGQAVGIKFVMEKAHFFESE"
     gene            825..1850
                     /locus_tag="ENO07_04430"
     CDS             825..1850
                     /locus_tag="ENO07_04430"
                     /inference="COORDINATES: protein motif:HMM:PF03372.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01247.1"
                     /translation="MKKSSNENQLTLNVMFYNIHLFGSTVPGADFFMRHVYRSPLIYE
                     DEARAKIAARKINKIGRDVDGCDIVGLCELWDDSMADIISNQVKQIFPYNYRPGRKSS
                     IRYVIGPGLMLFSKYPIIEMDFVPFKTNGNIIEKSCLKGVLRAVVELENNFPVTILMS
                     HFQAGQRSKERKVRLGQLSEIGAVLENIKSGPYGNAPIVLMGDFNIIAEDRNGNPRPE
                     YNAMTGVLGLKDIYRTKFKSALRNPGYTSHGGENNLLHLFHRNTRTRKRIDYILSNNY
                     RQMSIEDCGVDEFRTNNPIGNGTKKVRNPIRHLSDHYAVRARFLFEPDYVHGNVDRKF
                     SDQRWMN"
BASE COUNT          598 a          386 c          497 g          500 t
ORIGIN      
        1 gcgtacggaa ttgaaaaatc tgcaacgcag gctggaaatc acgactcttt acgttactca
       61 cgaccagacc gaggcgatga cattgggcga cagggtggcg attctgaagg agggggaaat
      121 cgagcagatc gggacgccgc tggagctata cgataagccc cggaatattt ttgtggccag
      181 gttcctgggt tcgtcaccga taaatatttt tgatgcagaa ataaccgctg aggaggataa
      241 aatcttcatc cggattgcgg gaaatagaat cgaactgccg cataaccgca tggaggaagt
      301 tcgtggattt aaggcggata aggtgatttt cggaatcagg ccggaggatg tggagccgca
      361 atctgatgcg gaaggatctc cggtcgcggg tgaaatacag gatatcgagc tcttgggcag
      421 agaaacctta ctgcatatct ccgttgacga taacactgtc agggccctga gcgaatcatc
      481 aaagtgggaa atcggacagg ctgtcgggat aaaatttgtg atggaaaagg cgcacttttt
      541 tgaatcagaa tgaaggctca atattctaag aaatatttcg acaatattta agatgcccgg
      601 gataccataa atatcaaatt tggaaattca aatcgccgta taaatccaaa cgtttcagca
      661 tataatggga acattcaata tggtgttcgg tgtttattaa agcttaactg ttcttggtta
      721 tcagcgggat aattgagaaa ataactggat cattttctga tttgaaagta taactttttt
      781 gatattttga gattgcaata gggctgtttc agtaaagggc gaccatgaag aaatcatcaa
      841 atgaaaatca acttacatta aatgtaatgt tttacaatat tcatctgttc ggcagcactg
      901 ttcccggagc agactttttt atgagacatg tttaccgcag tccgctgatt tatgaggatg
      961 aggcgagggc caaaattgcc gcgcgaaaaa tcaataaaat cggaagggat gtggatggtt
     1021 gcgatattgt agggctgtgc gaattatggg acgattctat ggctgacatt atcagcaatc
     1081 aggtgaaaca gatctttcca tacaattacc gtcccggcag aaagtccagt atcaggtatg
     1141 ttatcggtcc gggactcatg cttttcagca aatatccaat catagagatg gacttcgtgc
     1201 cgtttaaaac caatggcaat attatcgaaa aaagctgcct gaaaggtgtc ctgagggcgg
     1261 tcgtggaatt ggagaataat tttccggtga caatcctgat gtctcatttt caggccggtc
     1321 agagatcgaa ggagcgcaag gtcaggctgg gacaattgag cgaaatcgga gcggtcctcg
     1381 aaaatatcaa gtcaggcccc tatggcaacg ctccaattgt tttgatgggg gattttaaca
     1441 taatcgctga agatagaaat ggaaatccgc gtcccgaata taatgccatg acaggagtgc
     1501 tcgggctgaa agatatttac cgcacaaaat tcaaatcggc gcttcgcaac ccgggatata
     1561 catcacacgg cggggaaaat aatttactgc atttattcca tcgcaatacc aggacccgta
     1621 agcggatcga ttatatcctg tccaataatt atcgtcagat gagcattgag gattgcgggg
     1681 tggatgaatt cagaacaaat aaccctatcg gcaatggcac aaaaaaggtc agaaatccga
     1741 tcaggcatct ctccgatcat tacgcggtaa gggcgaggtt cttattcgaa ccagattacg
     1801 ttcatgggaa cgttgacaga aaattcagcg accagagatg gatgaattag agccttgctg
     1861 agctaatcaa tcctcccgat tcacgccagc atcaaataaa acttgaatat ggcatgcctt
     1921 gatagtaaat tcagggcatg ctgggtctta tcagacgaca tccactggcg gcaatactga
     1981 c
//
LOCUS       DSEB01000248            4012 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002165, whole genome shotgun sequence.
ACCESSION   DSEB01000248 DSEB01000000
VERSION     DSEB01000248.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002165"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(570..1469)
                     /locus_tag="ENO07_04435"
     CDS             complement(570..1469)
                     /locus_tag="ENO07_04435"
                     /inference="COORDINATES: protein motif:HMM:PF00482.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDS01248.1"
                     /translation="MDIYLIISFVFVSGFLLAFTLLYMYSRAKNPLRRRLGELQNNAE
                     YLKKKSNAARKKGLSLWGENNQQSSYIKLWLAKAGYRKPQSVSNYYGSRLLTTLFFTV
                     SAAMLGFYLHLNSAEMTAIIFVGVVVGAVIPKIWVMHKIVKRREEIRRAVPNVLDLMV
                     VCAEAGLSFTAAIHRIVNENRGSRNPLYEELHLLTQEFLIGGSKQDAFRNLADRTGVE
                     EIRSLAITLIQAEKLGTSIAESLRVLADSMRFKRRQNAEERANKTSVKLVFPLVLLIF
                     PELLVILVGPALIIMYKTLSTVV"
     gene            complement(1479..2450)
                     /locus_tag="ENO07_04440"
     CDS             complement(1479..2450)
                     /locus_tag="ENO07_04440"
                     /inference="COORDINATES: protein motif:HMM:PF00482.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01249.1"
                     /translation="MLIKIAILVFTAVFFVTFGLFLVLRKRMSSESRRLKMRMNGIAA
                     GGESQDDFLHSLIKDNKLSKIPLLNSFLMKFRFSRNLQLLITQSGVSVNLGTLVLSML
                     SLGALLLLFGYHVLSDMAIALALGTAGVAIPYLYLLLKKRKRLEEFENLLPEALDMIV
                     NALKAGFSFDSALKMVVQEIPDPLGLEMAITYEEQNLGIELPEALNNLKTRVPSDDLE
                     IFLTALIIHKKTGGNLAEVLGKTARTIRDRLRLKREVKTKTVHGRFSGMILILLPIIM
                     AAAIYLLNPDYVMVLIEEKIGNYLLIAAVIMQIIGIFVIRKIVNIRL"
     gene            complement(2462..3808)
                     /locus_tag="ENO07_04445"
     CDS             complement(2462..3808)
                     /locus_tag="ENO07_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HDS01250.1"
                     /translation="MNFRKNSEGERIPASQPFQSGDSETLQQRRDSFEELKTQLHQKL
                     VERLNLAALANLDQALLRAQIREITEILVAEENIFLSQENKERLINEIQNETLGLGPI
                     ERYTHDQDISDILVNTHGQVYIEKNGKLMLTETKFKSDSHVMLIIDRILSKVGRRVDE
                     SSPMADARLPDGSRVNAVIPPIAIDGPMLSIRKFRTDVLSMNDLLKNNSISMPMLQFL
                     QGAVKSRLNIIISGGTGAGKTTLLNILSGYIPTEERIITIEDSAELKLQQPHVIRLET
                     RPANIEGRGTVEQRDLVRNALRMRPNRIIIGEVRGAEIYDMLQAMNTGHDGSLTTVHA
                     NSSRDVLLRLETLMLLAGIEIREKAIRQLISSAIDIVIQINRFSDGSRKISSISEIVG
                     MEHDTITMQEIFKFDRTGVGEEGEVIGQYRATGIRPRSLDRILSSGVKLQTDVFNL"
BASE COUNT          966 a          999 c          931 g         1116 t
ORIGIN      
        1 actcattata ttataataga taacagggga agggtgcaat tgtgagcgaa ggtgcgtggt
       61 gaagaatttg atcctgaatt caaccggttc tgactttgta agggaatcgc cagaaactga
      121 attcgccata agcggttgct attattgcag tttagcccct ggcgccggct attgggtgca
      181 gagtttaaaa cggtggtgat ccgaaggtcg ccggttcaga tgcagttcct taacaaagat
      241 aagtttatga agtgcaccga tatcggggat atttttcggg attacgctcg aaaacatgac
      301 gatcttttgc ccgattcccg gagatccggg caataacaaa atgcccgatt ctcatgtctg
      361 aacacaagca tcaggcacct gtctcattat tgggaaggat ggataagtta tgaaatatgc
      421 cggatcaact tccgggattt caaaagttcc tgtgggtctg ctttttcagc ctcgaatttc
      481 tcctggcgaa gtaaagcaat cccaggatga ccgccacgac gatcagaatt atggttgttg
      541 acatgggaat ctccttacaa ttggttttct tataccacag ttgaaagtgt tttatacata
      601 ataatcagcg caggcccgac cagaatgacc agcagttcgg gaaatattag aaggaccagc
      661 gggaatacca gcttcaccga ggtcttgttg gctctttcct cggcattctg cctgcgtttg
      721 aacctcattg aatccgctaa gactctcaat gattcggcga tgcttgttcc cagcttctcg
      781 gcctggatca gggtgattgc caacgacctg atttcctcca ccccggtcct gtcggccaga
      841 ttcctgaagg catcctgttt ggaaccgccg atcaggaatt cctgggttag aaggtgcagc
      901 tcctcgtaca gggggtttct gctgccgcga ttttcattca ctattctatg aatggccgcg
      961 gtgaaactca ggccggcctc agcacagaca accatcagat ccaggacatt gggaaccgct
     1021 cttctgattt cttctctgcg cttaacaatt ttgtgcatga cccagatttt ggggataacg
     1081 gctcccacaa caacacccac gaatataatt gcggtcattt ccgccgaatt gaggtgcagg
     1141 tagaaaccga gcatcgctgc tgatacagta aaaaacaagg tggtcagcag cctgctgccg
     1201 taataattcg atacactctg gggctttctg tatccagcct tggccagcca taatttgata
     1261 taagatgact gctgattgtt ctccccccag agagagagac ctttcttgcg tgcggcattt
     1321 gacttcttct tcaggtactc cgcgttattc tgcaactctc ccaacctgcg cctgagagga
     1381 tttttggccc tggagtacat gtagagtaat gtaaatgcca gcaaaaagcc ggatacgaaa
     1441 acgaaggaaa taattaaata tatatccatt acatccacct atagcctgat gttgactatt
     1501 tttcttatga caaagattcc aattatctgc atgattaccg cagcaataag aagataatta
     1561 cctatcttct cttcaataag caccatcacg taatcagggt ttagcagata aatagctgcg
     1621 gccattatta tgggcagaag gatcaggatc ataccggaga acctgccatg cacggtcttt
     1681 gttttcactt ctcttttcag tctcagcctg tccctgattg tcctggctgt tttgcccaga
     1741 acctcggcca ggttgccgcc ggttttctta tgaataatca gcgcagtcaa gaatatttcc
     1801 aggtcatcgc tggggaccct ggtcttgagg ttattcaggg cctcgggcag ctcgatgccg
     1861 aggttttgtt cctcgtaggt tattgccatt tcgagtccca gaggatcggg tatttcctgc
     1921 accaccattt tcaatgccga atcgaaagag aatcccgcct tgagggcatt gacgatcata
     1981 tccagagcct ctggaagcag gttttcaaat tcttccagcc tcttccgctt tttcagaagg
     2041 agataaaggt atggtattgc tactccggct gttcccaatg ccagcgctat cgccatatcg
     2101 ctgagcacat gatatccgaa taacagcagc agagctccca gggagagcat gcttaaaacc
     2161 agtgtgccca ggtttaccga aactcccgat tgagtgatca ggagctgcag attcctggag
     2221 aacctgaatt tcattaagaa actgttcagc aatggtatct tgctgagctt gttatctttt
     2281 ataagcgagt gcaggaaatc atcctgactt tcgcccccgg cagcaattcc attcattctc
     2341 atcttcaagc gcctgctttc gctgctcatt cgcttgcgca ggaccagaaa gaggccgaat
     2401 gttacgaaga ataccgccgt aaaaaccaga attgctatct tgattaacat ggatagtgct
     2461 cctacaaatt aaatacatcc gtctgaagtt taactcccga cgaaaggata cggtccagcg
     2521 agcgcggacg gatccctgtc gctctgtatt gaccgattac ttccccctcc tcgccaactc
     2581 cggttctatc aaacttgaaa atttcctgca tcgtgatcgt gtcatgctcc attccgacta
     2641 tctcggaaat gctcgagatc tttcgggatc catcgctgaa gcggttgatc tgtattacaa
     2701 tgtcgatagc agagcttatc agctgccgaa tagctttctc gcgaatctca atgccggcca
     2761 gaagcatcag ggtctcaagt cgcagcagaa catccctgga gctgttggca tgaactgtgg
     2821 tcagggagcc gtcatgacct gtgttcatgg cctgtaacat gtcgtaaatt tcggctcctc
     2881 tgacctctcc gatgattatt cggttgggtc tcatcctgag cgcgtttcgg accagatctc
     2941 tttgctcgac cgtgcctctg ccctcgatat tggcaggcct ggtctcgagc ctgatcacat
     3001 gcggctgttg cagcttcaac tcggccgaat cctcaatagt aataatccgc tcctcggtcg
     3061 gaatatatcc cgacaagata ttcagaaggg tggttttacc ggcgccggtc ccgcccgaga
     3121 taatgatgtt caatctgctt tttaccgcgc cctggaggaa ttgcagcatg ggcatggaga
     3181 tagaattgtt tttcaaaagg tcattcatgc tgaggacatc agtcctgaac tttctgatag
     3241 aaagcatcgg accatcgatc gcgatgggtg gaattaccgc attcacacgg gagccgtctg
     3301 gcagccgcgc atcagccatg ggcgaagatt catccaccct tcggccgact ttggataata
     3361 ttctgtcgat gatcagcatg acgtgactgt cgcttttaaa ttttgtttcg gtaagcatca
     3421 atttgccgtt tttctcgata tacacctggc cgtgggtatt gaccaggata tcggatatgt
     3481 cctgatcatg ggtatatctt tcaatcggcc ccagcccgag agtttcgttc tggatttcat
     3541 ttatcagccg ctctttgttt tcctggctta gaaaaatatt ctcttccgcc accagtattt
     3601 cggtgatctc ccgaatctgt gcgcgcagca aggcctggtc cagattggcc agcgctgcca
     3661 aattgagcct ttcgaccagc ttctggtgga gctgggtctt caattcctca aagctatcgc
     3721 gcctttgctg cagggtctct gaatccccgc tctgaaaggg ctggctggct ggaattctct
     3781 ctccctctga gttctttcta aaattcataa cttaaatccc aatctaagat taactgcttc
     3841 ttttggtttt gtggctaaag tttccattag tcataaccct ggcaagtttg aaaatgctct
     3901 tgcctacatt ggtcatgcgt ttctccccga tcaaaggcaa tccggagtcg atagcgttga
     3961 ttgaagcctt gtaattgttc ggaatcgacc agaacaccgg gaaatcaaga at
//
LOCUS       DSEB01000249            1873 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1011721, whole genome shotgun sequence.
ACCESSION   DSEB01000249 DSEB01000000
VERSION     DSEB01000249.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1011721"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(36..1427)
                     /gene="dnaB"
                     /locus_tag="ENO07_04450"
     CDS             complement(36..1427)
                     /gene="dnaB"
                     /locus_tag="ENO07_04450"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDS01251.1"
                     /translation="MTAERSHQGSNPHSIEAEQAVLAAVLNFRDAIFEVTDVIRQPEV
                     FYDPRHSFIFEASIQLFEDNKPIDLITISEQLQKNQKLEKIGGRSYLAELTGHIASTA
                     NVRHHANIVYDKYLLRRLIEISHEITEDCRSGTLEAEDLLDSAENSIFSLSQNRLGDG
                     FINIGSILPDTFEQIEEYHKSGGGITGIATGYIDLDNITAGLHPGDFIVVAGRPSMGK
                     TGLGLNILENIAIDSKKSVAMFSLEMSKQQIALRLLCSKARINAHKMRTGRLPDSDWP
                     KLSSAVGPLSEAGIFIDDTPNIGILELRSKARRLKAKEDIQLLIIDYLQMMQGPRGAD
                     NRQQEIAMISRSLKGIAKELDIPVIAVSQLSRGVEIRGGDKRPQLADLRESGAIEQDA
                     DVVMFVYRPEFYGIEFEKDGTPTDKLAEIIIAKQRNGPTGKVKLTFLKDFIRFENRTE
                     IYSEVEEATDTPF"
     gene            complement(1514..>1873)
                     /locus_tag="ENO07_04455"
     CDS             complement(1514..>1873)
                     /locus_tag="ENO07_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDS01252.1"
                     /translation="AIDFERSEVYIANIVKCRPPNNRDPESSEMETCMPYLLKQIEMI
                     NPHFICCLGRIAAQALLDTRLPLGKLRGGFHDWNDRKVMVTYHPAALLRFPQYKPHTW
                     EDVQMLRKAYDEFKGSS"
BASE COUNT          440 a          484 c          469 g          480 t
ORIGIN      
        1 atccgcccaa gactttcaat tagttttctc atggcttaga acggcgtatc cgtagcctcc
       61 tcaacctcag agtatatctc ggtcctattt tcgaaacgga tgaagtcttt caggaatgtc
      121 agctttacct tgccggtcgg accatttcgc tgcttggcaa tgataatctc ggccagcttg
      181 tccgtggggg taccgtcttt ctcgaactca atgccgtaaa attccggcct gtaaacaaac
      241 atgacaacgt cggcatcctg ctcgattgcg ccggattcac gaagatcggc gagctgggga
      301 cgtttgtcgc ccccgcgtat ctccactccg cgcgataact ggctgaccgc tatgaccggt
      361 atatccagct ctttggcgat gcctttcagc gagcgggaaa tcatggcgat ttcctgctgg
      421 cgattatccg cacctctcgg accctgcatc atctgcaggt agtcgatgat cagaagctga
      481 atatcctctt tggctttcag gcgcctggcc ttggagcgga gttcaagaat cccgatattg
      541 ggcgtgtcat cgatgaaaat tcccgcctcg gaaagagggc cgacagcgct ggaaagcttg
      601 ggccagtcgg agtcgggaag ccgcccggta cgcatcttgt gcgcattgat ccgcgcctta
      661 ctgcacaata atctcagcgc aatctgctgt ttggacatct ccagcgaaaa catggccaca
      721 gatttcttgc tgtcgattgc gatattttcc aggatgttca ggcccaggcc ggtcttgccc
      781 atcgatggtc ttccggctac aactatgaag tcccccggat gaagccccgc ggtgatattg
      841 tccagatcaa tatagccggt ggcaatgccg gttatgcctc cgccagattt atgatactcc
      901 tcgatctgtt cgaatgtatc cgggaggatc gagccgatat tgatgaaccc gtctcccaga
      961 cggttctgtg ataagctgaa aatcgaattc tctgccgaat ccagcaggtc ctcggcttcc
     1021 agagtacccg aacggcaatc ctcggtgatt tcgtgcgaaa tttcgattaa acggcgcagg
     1081 agatatttat catagactat attggcatgg tgacggacat tggcggtgga ggcgatgtga
     1141 ccggtaagtt cggccagata tgaacgcccg cctatttttt cgagcttctg atttttttga
     1201 agctgctcgg aaattgtaat caggtctatc ggcttattgt cttcgaaaag ctgaatggag
     1261 gcctcaaaaa taaagctgtg gcggggatca tagaatacct caggctgcct gatcacatcg
     1321 gtaacttcaa agatcgcatc cctgaagttc aggacagccg ccagaaccgc ctgttccgcc
     1381 tctattgaat ggggatttga tccctgatgt gaacgttctg ccgtcatatc cctgacctgt
     1441 attcagcaaa ttaagatatc ttgaataaaa tttaattata caaaaagccc cgcaaaaatc
     1501 caagcttata aaactatgat gaccccttaa actcgtcata tgccttgcgg agcatctgca
     1561 catcctccca tgtatggggc ttatattgcg gaaagcgcaa aagagcggca ggatggtaag
     1621 tcaccatcac cttacgatcg ttccagtcat ggaaacctcc ccgcagcttt ccgaggggaa
     1681 gcctggtatc cagaagcgcc tgagccgcga tccgccccag acagcagatg aaatgcggat
     1741 taatcatctc aatctgctta aggaggtatg gcatgcaggt ctccatttcg gatgattccg
     1801 gatcgcggtt gttgggagga cggcatttga cgatgttggc gatgtatact tcggatcgtt
     1861 caaagtctat agc
//
LOCUS       DSEB01000250            3977 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002204, whole genome shotgun sequence.
ACCESSION   DSEB01000250 DSEB01000000
VERSION     DSEB01000250.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002204"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..637
                     /locus_tag="ENO07_04460"
     CDS             <1..637
                     /locus_tag="ENO07_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDS01253.1"
                     /translation="PYSVVLLDEIEKAHPDVFNILLQLFDDGSLTDAFGRRVDFRNTI
                     IIMTSNIGTRRIGEGKSMGFAKEKVESDFEIMKKNVLEELRKIFNPELLNRIDETIIF
                     HSLTREHIKEIVEIRLLEVSQRLAEKGISFYLTEKAKDFLADKGYDKTFGARPLNRAI
                     QKFLEDPLAEEILRGQYAGDCEVEVDYEDGDALTFKITTKIESEDKKPVSQ"
     gene            724..978
                     /locus_tag="ENO07_04465"
     CDS             724..978
                     /locus_tag="ENO07_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDS01254.1"
                     /translation="MAHKKGVGSSRNGRDSHGQRRGVKAFAGQNVTAGSIIVRQCGTR
                     IHPGENVGMGKDYTLFSKIDGIVHFDRKGKDKKVCSVAAN"
     gene            complement(1162..2331)
                     /locus_tag="ENO07_04470"
     CDS             complement(1162..2331)
                     /locus_tag="ENO07_04470"
                     /inference="COORDINATES: protein motif:HMM:PF00155.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDS01255.1"
                     /translation="MSTKPRISNRVSQIPKSAIHEMTRLSKEVEDVAFLSWARPTAGT
                     PKHIGEAAIKAIIDGSVGGYSENAGLVRLREAIVEKLKRDNQVEAEISQILVTVGAIE
                     GLSAAVMAIIDPGDEVLLFTPTYSTHIRQVVIASGTPVLVPTIEEKGFALDFDAIKKA
                     ITPKTKAIFCCSPNNPTGTVFSKEDLQKLAEIALQHNLMIITDEAYEYFVYDGLEHFS
                     LASIPEMKRHVISCYTFTKTYAMTGWRIGYLHADESLIPQITKAHIPFAICAPVVSQY
                     AALAALEGPQDCISEFKAHYLAARDLMCQRLDQLDSIFSYHKPGGSYLMFPKILLEEG
                     RDSMTFCKDLLERGKVSTTPGIAFGPTGESHLRLSFCVSDEEINKAFDRIENLFL"
     gene            2580..3421
                     /locus_tag="ENO07_04475"
                     /pseudo
     CDS             2580..3421
                     /locus_tag="ENO07_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
     gene            3434..>3977
                     /locus_tag="ENO07_04480"
     CDS             3434..>3977
                     /locus_tag="ENO07_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HDS01256.1"
                     /translation="MSNGKRTIETPLTDELVRELTIGTEVLIRGTIYTGRDSAHKRMT
                     ESLAKGEELPFDPKGQIIYFAGPTPPKPGKVIGSAGPTTSYRMNKYAPDMLDAGLKGM
                     IGKGEMSPEVAGKLKEHTAVYFLAIGGAGALISKSIQAAEVIAYEDLGPEAVRKLTVS
                     EFPAIVVMDCFGGNLFREGVE"
BASE COUNT         1071 a          911 c         1012 g          983 t
ORIGIN      
        1 tccatattcg gtggtacttt tggacgaaat cgagaaggcg catccggatg tattcaatat
       61 cctgcttcag ctttttgatg acggttcgct taccgatgcg ttcggcagaa gggtcgattt
      121 cagaaacacc attataatca tgacctcaaa tatcggtact cggcggattg gcgagggcaa
      181 atcaatgggc tttgccaaag agaaagtcga aagcgatttc gagatcatga agaagaatgt
      241 tctggaagag ctgagaaaga tattcaatcc ggagcttctg aatagaatag atgaaaccat
      301 tatcttccat tcacttacga gagagcacat taaagagatc gtggaaatca ggctgctcga
      361 ggtttcgcag cgtctggccg agaagggcat ctccttctac ctgactgaga aagccaagga
      421 ctttctggcc gataagggct atgacaagac tttcggcgcc cgtccgttga atcgcgccat
      481 ccagaagttc ctcgaggatc ctctggcgga agagattctg cgcggccagt atgccggtga
      541 ttgtgaggtc gaggtcgatt atgaggatgg cgatgcgctg accttcaaga tcacgaccaa
      601 aattgaatcg gaagataaaa aaccggtttc acagtaattt tgacttgcca gagttatggt
      661 aattgattat aatctaagac ccaaatacat gatattcggg ttttgggttt caggaggaaa
      721 aaaatggctc ataaaaaagg tgtcggttca tccagaaatg gccgcgattc acacggacag
      781 agacgtggtg ttaaggcttt cgccggacag aatgtaaccg ctggatcgat tatcgtgcgt
      841 cagtgcggca ccagaataca tcccggtgag aacgtcggca tgggcaaaga ttacactttg
      901 ttttccaaaa ttgacgggat tgttcatttc gaccgcaaag gcaaagacaa aaaagtctgc
      961 agtgtagcgg ccaattaaga tagatattgg gagattatct cctcttttcg acaccccgtc
     1021 tacggggtgt tttttttgga gagatcctta ccacggtcaa ttctctaaga cgcttctgta
     1081 agaggtgcag actctcaaag ctgaaaagat atcagaataa actgctataa atcaagtctg
     1141 tccgttgcag attccgcagt attacaagaa aagattctct atcctgtcga aagccttgtt
     1201 tatttcttca tcggaaacgc agaacgatag ccttagatgg ctctcccctg tcggcccgaa
     1261 ggcgattccg ggggtcgttg acactttccc cctttccaat agatccttgc agaaagtcat
     1321 ggaatccctg ccttcctcca gaaggatttt gggaaacatc agatacgaac cgcccggctt
     1381 gtgataagaa aaaatcgaat ccagctgatc cagacgctgg cacataagat cacgtgcggc
     1441 gaggtaatgc gccttgaatt cggaaatgca gtcctgtggg ccttccaggg cggcaagagc
     1501 agcatactgt gatacaaccg gggcgcagat cgcaaacgga atatgtgcct ttgtgatttg
     1561 aggaattaaa gactcatccg catgcaaata gccgatacgc catccggtca tggcataagt
     1621 tttggtgaac gtatagcagc ttataacatg tctcttcatt tcaggtatcg aagccagcga
     1681 gaaatgttcc aggccatcat agacaaagta ctcgtaagct tcgtccgtta ttatcatgag
     1741 gttatgctgc aaagcaattt ctgctaattt ttgcagatcc tctttggaaa aaacagttcc
     1801 ggtcgggttg ttgggtgagc agcagaaaat tgctttcgtt tttggcgtaa tcgccttctt
     1861 tatcgcgtca aagtcaagtg cgaaaccctt ttcctcgata gtgggaacta ataccggtgt
     1921 acccgaggca atcacaacct ggcggatgtg ggtagaatat gttggtgtga acaggagcac
     1981 ttcatccccg ggatcaatta tcgccatgac agcggcggaa agaccctcga tagcgcccac
     2041 tgtcaccaga atctgggaaa tctccgcttc gacctggttg tctcttttaa gcttttctac
     2101 gatcgcctca cgcaacctga caagtccagc gttttcggaa taaccgccga cagaaccgtc
     2161 tatgattgcc ttgatcgccg cctccccgat gtgctttgga gttcctgcgg tcggcctggc
     2221 ccaggagaga aatgcaacgt cttctacttc ttttgataac cgcgtcatct catggatggc
     2281 cgattttggt atttgtgaca ccctgttaga tattcttggt tttgtgctca cttcgcgacc
     2341 ttcctttatt taattttcaa agtagctaca ttaaatccgg acagttttgt ccgcttccag
     2401 tttgaaactt aagcaagata tccagctttt gcaagtagtt tgaaatgata ccgcaaatgg
     2461 gtttaatatc cgtgcctcaa tattcatccg tattaaatga taatcttatc ttcaatccat
     2521 tgacaaattc cgtttttgta atcatattcc aaaaaaccga aacagagagg atggcataaa
     2581 tgagagagct acatgtttct gagattaccc ctgcggtcag gaaactctgc atggaagcca
     2641 attatgatct gggagatgat gtttataaca ggcttaaaag tgcaatcgat acagagaaat
     2701 cgccactggg gaaatcgatc ttaaacgaca tcgtaaaaaa tgcagaaata gccagagaag
     2761 agcaggtacc gatctgccag gataccggtt tctcggtgtt cttctcaaga tgggacagga
     2821 cctgcatata accggcggag acttcaatga ggccataaac gagggggttc gccagggtta
     2881 taaagaaggc tatttgcgta aatcgattgt gaccgatccg cttgaacgaa aaaacagcgg
     2941 tgacaacacc ccggcctcaa taattgtgga gatagtcccg ggtgacaagc ttgaaatcat
     3001 tttcgccccc aaaggcggcg gagcggaaaa tatgagcgag gtcaagatgc tcaaaccttc
     3061 tgacggtgtt gaggggctga aggattttgt agtcgagcgt gtggccaaat ccggaggcaa
     3121 tccctgtccg ccagttgtcg taggagtcgg tatcggcggc acattcgacc tgtgcgccca
     3181 gctggccaaa aaggcgctga tgcgcgaaat cggcagcaaa catccccagg agttttacgc
     3241 caaactggaa gaggagctgc tggagctggt taataaaaca ggtgttggcc ctcagggcct
     3301 cggtggtcgt acaaccgcgc tggctgtaca tgtggaggta catccctgcc acatcgccag
     3361 tttccccgta gccgtcaata tgcagtgcca tgccgcccgt cacaaaaaag tcgttttata
     3421 aggagaattg acaatgagca atggaaaaag aactatagag actcctctga ccgatgagct
     3481 ggtcagggaa ctcacgatcg ggactgaggt actgatcaga ggcactatat acacaggccg
     3541 tgattcggcc cataaacgta tgaccgagtc cctcgccaaa ggtgaggagc tgccattcga
     3601 tcctaagggg cagattattt atttcgccgg acctacgccg cccaaaccgg gcaaggttat
     3661 cggatcggcc ggccccacca cctcctacag gatgaacaag tatgctcccg atatgctgga
     3721 tgccggtctt aagggcatga tcgggaaagg cgagatgtcg cctgaggtgg ccggcaagct
     3781 gaaagagcat accgccgtat atttccttgc aataggcgga gcaggggcat tgatatcaaa
     3841 atcgatccag gcggctgagg tgatagccta cgaggatctt ggcccggagg ctgtcaggaa
     3901 attgaccgtc agtgaattcc cggctatcgt tgtcatggat tgctttggcg gcaatctctt
     3961 cagagaaggc gtcgaga
//
LOCUS       DSEB01000251            4274 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001897, whole genome shotgun sequence.
ACCESSION   DSEB01000251 DSEB01000000
VERSION     DSEB01000251.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001897"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            32..892
                     /locus_tag="ENO07_04485"
     CDS             32..892
                     /locus_tag="ENO07_04485"
                     /inference="COORDINATES: protein motif:HMM:PRK04375.1-2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HDS01257.1"
                     /translation="MKSNWRSYIDLTKPRVMVLVVLSGITALFLEGTAVSHPLDALLI
                     TLGIYLAGGSANALNQYFEREIDARMSRTRNRRPLPLGNIEPPNALSFAIIIGLASIA
                     LFGLGYNSLSALLALGTIIFYGFFYTLWLKPNTDQNIVIGGAAGAMAPLIAWAAASGS
                     LAIAPAILFLIVFIWTPPHFWALALYFKDDYRKVGLPMLPIARGEKSTLNQIFFYTIA
                     LFAVSLALLAVSAGPIYAIAAILLGGIFVKKAYSAKSKKSRELQWALFGYSIVYLFGL
                     FFAMIIDSFI"
     gene            926..1495
                     /locus_tag="ENO07_04490"
     CDS             926..1495
                     /locus_tag="ENO07_04490"
                     /inference="COORDINATES: protein motif:HMM:PF04442.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01258.1"
                     /translation="MKRDSKILLVLVAVAIAMFIFGYANVPLFKRFCSAIGIDLSPNT
                     EVAAETSEIEIDSTRQLKVLFTTSVNDDLPIVFESKKHQSEVFVGESDKNMYHFVNMT
                     DDTLYFRPVHSVFPADASKKYSMIECFCFRDMMLLPHQEVEVPLVYYFKPSLSEEVNR
                     VTMHYTLFKRDRDDVDLTGTPGEFNMETN"
     gene            1661..2455
                     /locus_tag="ENO07_04495"
     CDS             1661..2455
                     /locus_tag="ENO07_04495"
                     /inference="COORDINATES: protein motif:HMM:PF00510.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HDS01259.1"
                     /translation="MNEITARDHGIHLPPKSIWPLILALGIGFLTFGVIFIASSRPIF
                     AELSFIVGGSVTVVSLMGWAHSVVKEKAEPGEEARAVQQKDLLMFLKYFLISEAAIFG
                     ALFVHYYNNRFGQEYWPLAGSPEIHTTLPAVATLILMFSSLTCEIGHLALKKGRRFRS
                     KTFILITAVLGLIFLGFQGYEWGLLINQYSFTAETNIYGTLFYMLTGFHGLHVSVGIL
                     FLILVYGRLEMGQMDQKRHFSMIAATWYWHFVDVIWILLFFSIYLI"
     gene            complement(2436..3413)
                     /locus_tag="ENO07_04500"
     CDS             complement(2436..3413)
                     /locus_tag="ENO07_04500"
                     /inference="COORDINATES: protein motif:HMM:PF00892.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDS01260.1"
                     /translation="MTLKPLLPARGGFSYNSVTMNQAPAGKKIISDAGLLYAAAVWGS
                     TFFIVKGSLEFIDPIILVGYRFIIASALVALLCLIGKRPLFRNMKKGMTLGIFIWLLY
                     VPQTIGLGITTASNSGFITGLFVAFVPIFSFVIFRRVPSLLGIIATLISLSGLWILTG
                     GLTDINRGDILTLLAAMTYAIHILYVDKFLKEGDDPFTLCFQQFLFVGIASVAVGVIA
                     GLPFGWGNAGTGWTVLFLAVFPTFSAFFIQVVAQKYTTPIRVSLIFAFEPVFAAIFAW
                     TLGGEQIILHRAMGGLLIFLAMIISGLPRDILNFEKRKAVSEEIKSDKY"
     gene            complement(3431..4234)
                     /locus_tag="ENO07_04505"
     CDS             complement(3431..4234)
                     /locus_tag="ENO07_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01261.1"
                     /translation="MSMINEFAGKLDSETKSERIAYAGRKRIVERLNDREKGVSDPVY
                     VGLVNNNTSNGVMVFNARSADKIDTTDESVLRYLAGNLYGGGGGHGLFMRTWGAGLAY
                     SNGYGAGALSGLVSYYAERCPNIAETMRFVVDVLNNAETDPKLVDYAIAQAFSHSRAP
                     SPYESRGSQMASDLEDGYYPEKVKAYRQKILELKENRDLTKELFSRMEEAYGPVLIGY
                     GKPSSESRDGSFFIIGPDAQFQTLEEYIETVEGPQKVYKLYPRDFWLTI"
BASE COUNT         1073 a         1047 c         1038 g         1116 t
ORIGIN      
        1 atgctatata taacctatga gttgaagaat catgaagtcc aactggcgta gttacataga
       61 tcttaccaaa cctcgcgtta tggttctggt tgtgctttcc ggaataacgg ctttgttcct
      121 ggaaggtacg gcggtttctc atccacttga tgccctgctg ataaccctgg gcatctatct
      181 ggcgggaggt tccgccaatg cgctgaacca gtatttcgag cgggaaatcg atgccaggat
      241 gagccgcacg cgcaacagac ggccgctgcc tctgggaaat atagagcccc ccaacgcact
      301 ttcttttgcg atcatcattg gtcttgcatc gattgcgcta tttggtctgg gatataactc
      361 gctctccgcg cttctggcgc tgggtacgat aatattctac ggctttttct ataccttatg
      421 gctcaagccg aataccgatc agaacatcgt aatcggcgga gctgccgggg ccatggctcc
      481 cttgatcgca tgggcggcag cttccggatc gctggcaatc gcgccggcta tactatttct
      541 gattgtgttt atctggaccc cgccgcattt ctgggctctg gctttgtatt tcaaggacga
      601 ttacaggaaa gtcggcttac cgatgctgcc gatagccagg ggggaaaaga gcacattgaa
      661 ccagatattc ttctacacca ttgccctttt cgcagtcagc cttgcgcttt tggcggtcag
      721 cgccgggccg atatatgcga tagcggcaat cctgctgggc ggtatatttg tcaaaaaggc
      781 ctatagcgca aagagtaaga agagccggga gctgcaatgg gcactattcg ggtattcaat
      841 cgtttattta tttggattgt tctttgcaat gataatagac agcttcattt agaagttgta
      901 atataaattg atcgaggtga gcgagatgaa gagagacagt aagatattat tggttttagt
      961 tgcggtcgca attgccatgt ttatatttgg atatgccaac gtaccgcttt tcaagcgctt
     1021 ctgttccgca atcggaatag atctttctcc caataccgag gtggcggcgg agaccagcga
     1081 gattgaaatc gattctacaa ggcaattgaa agtgctgttt acgacatcgg tcaatgatga
     1141 tttgccgatc gtttttgaat ctaaaaagca tcaatccgag gtttttgttg gcgaaagcga
     1201 caaaaatatg tatcacttcg ttaacatgac ggacgatact ctgtattttc gccccgtgca
     1261 ttcggttttt ccggccgatg cttcgaaaaa atacagcatg attgaatgct tctgcttccg
     1321 ggatatgatg cttcttccgc atcaagaggt cgaggtgccg ctggtttatt atttcaaacc
     1381 ctctctcagc gaggaggtta atcgcgtaac tatgcactat acgcttttta agcgggatag
     1441 agatgatgtc gatctgaccg gtacaccggg cgagttcaac atggagacca attaggatgc
     1501 gcaaagagcc gctggatcca aaaaccagaa agcgaaatat catcctggcg attatcctgg
     1561 ccgttatcat tatattcgtg gcggttttca cagcccagag tatctacaaa gcggatttcg
     1621 gcggtcagga acaggatgaa aatgtagaga tgggatattg atgaacgaaa ttaccgccag
     1681 ggatcacggc atacatctgc cgcccaaatc aatatggccg ctaattcttg cgctgggcat
     1741 cggctttttg acgttcgggg tgatcttcat agcctccagc agacctattt ttgccgaact
     1801 cagctttatt gtaggaggtt cggtcacagt tgtgtccctg atgggatggg cgcatagtgt
     1861 ggtgaaggaa aaggccgagc cgggcgagga ggcgcgcgcc gttcagcaaa aagatttgtt
     1921 gatgttcctc aaatatttcc tgatttcgga ggccgctatc tttggcgcct tattcgtcca
     1981 ctactacaac aacagattcg gacaggaata ctggccgctt gcaggttcgc ccgaaatcca
     2041 taccaccttg cccgcagtcg cgaccttgat tctgatgttc tcctcgctga catgtgaaat
     2101 cggccacctg gccctgaaaa agggcagacg attccgttcc aagacattca tcctcattac
     2161 agccgtattg ggtctgatct ttctcggctt ccagggctat gaatggggcc ttctgatcaa
     2221 ccagtacagt tttacagcag agacaaatat ctacggcacg ctattttaca tgctgacagg
     2281 ctttcacggt ttacatgttt cagttggcat ccttttcctg atccttgtct atggcaggct
     2341 ggagatggga cagatggatc agaagcgcca tttcagcatg atagctgcga cctggtactg
     2401 gcattttgtg gacgtcatct ggatcctgtt attcttcagt atttatctga tttaatctcc
     2461 tccgacacgg ctttcctctt ctcaaaattc agtatatctc ttggcaggcc ggaaattatc
     2521 attgccagaa atatcaagag acctcccatg gcacgatgca gtattatctg ctcgcctccc
     2581 agggtccacg cgaatatagc cgcgaacaca ggctcgaacg caaagatcag ggagaccctg
     2641 atgggcgtgg tatatttctg agccacaacc tgaataaaaa aggccgagaa agttgggaag
     2701 accgccagga acaagaccgt ccatccggta ccggcattac cccacccaaa cggcagcccg
     2761 gcgattactc cgactgcaac gctggctatt cccacgaata aaaactgctg gaagcacaat
     2821 gtgaacggat cgtcgccttc tttcaaaaat ttatcgacat ataaaatatg tatggcatag
     2881 gtcatggctg caaggagcgt caggatatca cctctattga tatcggtcaa tccgcccgtt
     2941 agaatccata aaccggacag cgaaatgagg gtcgctataa tccctaacag tgatggtacc
     3001 ctgcggaaga tcacgaatga gaagatgggc acgaaggcta caaaaagccc tgtaataaag
     3061 ccggaattcg aggcagtggt gattcccagg ccgatcgtct gcgggacata caggagccag
     3121 atgaaaattc cgagtgtcat gccctttttc atatttctga acaggggcct tttgccgata
     3181 aggcataata gcgccaccag cgctgaagcg ataatgaatc tgtacccgac caggatgatt
     3241 ggatcgatga attccaggct gcccttgaca ataaaaaacg tggagcccca gaccgcagcc
     3301 gcatacagca gcccagcatc ggaaatgatt ttttttccag cgggagcttg attcatagtg
     3361 acgctattat acgaaaaacc gcccctggca ggcaagagcg gttttaatgt catttaattt
     3421 atagagaata ctaaattgta agccagaaat ctctgggata cagtttatag accttctgcg
     3481 ggccctcgac tgtctcgata tactcttcca gggtctggaa ctgcgcatcg gggccgatta
     3541 taaagaagct gccgtccctg ctttccgatg agggcttgcc gtacccgatc agcaccggcc
     3601 cgtaggcttc ctccatgcgc gagaaaagtt ctttggtgag gtcacgattc tcttttaatt
     3661 ccagtatctt ctgacggtag gcttttacct tctcaggata ataaccgtct tccagatcgg
     3721 aggccatctg cgaaccgcgg gattcatatg gggacggcgc ccgtgagtgt gaaaacgcct
     3781 gggcaatggc ataatccaca agcttaggat cagtttcggc attattgaga acatccacaa
     3841 caaaacgcat ggtttctgct atatttggac atctttcggc ataatatgag actaaaccgc
     3901 ttaaggctcc cgcaccataa ccattgctgt aggccagccc cgcaccccat gtacgcataa
     3961 acagaccatg tcctccgccc ccgccataaa gattgccggc caggtatcgc aataccgatt
     4021 catcggtagt gtcaatctta tctgcgctac gggcattaaa gaccataaca ccgttgctgg
     4081 tattattatt taccaggccg acatagacag gatcgctgac acccttttca cggtcattaa
     4141 gcctctcgac aatccgtttt ctgccggcat aggcaatccg ttccgatttc gtttctgaat
     4201 ccagtttgcc cgcaaattcg ttaatcatac tcatagtggc ggcgcggtct gttgaattcg
     4261 agatcatata cata
//
LOCUS       DSEB01000252            1861 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1011929, whole genome shotgun sequence.
ACCESSION   DSEB01000252 DSEB01000000
VERSION     DSEB01000252.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1011929"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            560..925
                     /locus_tag="ENO07_04510"
     CDS             560..925
                     /locus_tag="ENO07_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01262.1"
                     /translation="MEGISSDFDIFARGILDSLLLFLVIPFSIPTFIGYHFLALKRNK
                     HAGIAFIFSFLIALVYIAWIGFLPTLVIFFYISAFYLLVMGPFWLLDKLIRWYENKYI
                     YEKYRKYLDDRKTESNSNG"
     gene            complement(928..1791)
                     /gene="prmC"
                     /locus_tag="ENO07_04515"
     CDS             complement(928..1791)
                     /gene="prmC"
                     /locus_tag="ENO07_04515"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDS01263.1"
                     /translation="MPVKLIDIINSFDKILDVAGVEAPRQNVERMLEKILGLSKVDLY
                     LDPKRQVSDEDAAQLRGLIDRRLNNEPLQYILGETEFYGIRLKCDRRALIPRPETEFV
                     VSKGLNLIKDKKGLNILDLACGSGNISIAMAASSPYQNYYASDLSLDAVSLARENAEL
                     NDLADRIEFHQGNLFEPFKKKDIVFDMILANPPYIKESELAKLHEQVRKYEPQLALTS
                     GQDGMDFIRQMLTEAPEYLNRRAYLIFEVAFDQMDTIRDLAENETKFDVVEVVEDYSG
                     IERVIVLRMGE"
BASE COUNT          497 a          467 c          358 g          539 t
ORIGIN      
        1 ccataagatg ccccgaaaca tacggatatc gattcataac tacaaatact ttctctccgc
       61 gatagagcac ataattctgt ctatccttct cttccctgaa aatcctgcag aataaacagc
      121 ccttctcctt ggggcccaga atatactctt ccctccacgg cgcgtacatc ttcctgtcca
      181 taaaaccata ataaaaaagg cggcttcttt tggcaaataa aaataaaagc ctggaaatac
      241 aatctgttta tcgacagtat cttcgggact gatactgaga ttcatccaat tattggcctt
      301 acctaatatc tgctatgcag ttacatccac tcaataataa taacggtcag gaaagtctga
      361 attttcggga tatgaaccgc aacttgctca tatacttttg attagagaca atcgatcccg
      421 cgataatcct tcgcggcttc atgcaggctg aattttttcc acggctttca tgcaattgcg
      481 caggaagata tcaagacagc atccattttt tattaaacat tgcaatcaag atttcatata
      541 aacttaatga ggaatataga tggaaggcat tagttctgat tttgacattt tcgcacgcgg
      601 aatactggat tcgctgcttc tgttcctggt gatacccttt tctatcccga cctttatcgg
      661 ttaccatttt ctggccctca aaagaaacaa acatgccggt atcgccttta tcttcagctt
      721 cttgatagcg ctggtgtata tcgcctggat cggctttttg ccgacactgg taatattctt
      781 ttacatttcc gcattttatc tattggtgat ggggcctttc tggcttctcg ataaacttat
      841 cagatggtat gaaaacaaat atatttacga aaaatacagg aaatacctgg atgacaggaa
      901 aacggaatca aattcaaatg gctgaatcta ctcgcccatt ctcagcacaa tcaccctctc
      961 gatcccggaa tagtcttcaa ccacttcgac cacatcaaac ttcgtttcat tttctgccaa
     1021 atcccttatg gtgtccatct gatcgaaggc gacctcgaaa ataagatagg cccggcgatt
     1081 caagtattcc ggagcctcgg tcaacatctg cctgatgaaa tccatgccgt cctgccctga
     1141 tgtcagggcc agctggggtt catattttct gacctgctca tgcagttttg cgagctcact
     1201 ttccttgata taagggggat tcgccaggat catatcgaag actatatctt tctttttaaa
     1261 tggctcgaat aggtttccct gatgaaattc tattctgtcg gccaggtcat taagctcggc
     1321 attctccctg gccaatgaga ccgcatccag ggatagatca gaggcatagt aattctggta
     1381 gggactgctg gccgccattg caatcgaaat attacccgag ccacaggcca aatcgagtat
     1441 attcagtccc tttttgtctt ttataaggtt caatcctttt gatacgacaa actccgtttc
     1501 gggacgggga atcagcgccc gcctatcaca tttcaacctt atgccgtaga attccgtttc
     1561 gcccaggata tactgcagcg gttcgttgtt caacctgcgg tcgatcaggc cccgcagctg
     1621 tgcagcatcc tcgtccgaga cctggcgctt tggatcgagg tacaaatcga ccttgctcag
     1681 gcccagaatt ttctccagca tccgctccac attctggcgc ggggcctcca ccccggctac
     1741 atcaagaatc ttatcaaatg aatttatgat gtcgatcaac ttgactggca tagattataa
     1801 aacttcagaa ggaagattgt aatcgttaca acagctccag cccgaacatc catgatactt
     1861 a
//
LOCUS       DSEB01000253            3831 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002382, whole genome shotgun sequence.
ACCESSION   DSEB01000253 DSEB01000000
VERSION     DSEB01000253.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002382"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1174
                     /gene="priA"
                     /locus_tag="ENO07_04520"
     CDS             <1..1174
                     /gene="priA"
                     /locus_tag="ENO07_04520"
                     /inference="COORDINATES: protein motif:HMM:PF00271.29"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDS01264.1"
                     /translation="GKSVSCPVVMGSATPSVESWHNVKSGKYKLLRLGSRWDSRTLPK
                     IQPVEFAFSPAGSSISDELLQKMNDVLKKDGQVMLLLNRRGFAPTVKCSDCGHSLKCP
                     NCSVGLVYHRSSNSLKCHFCDYQSRTTDTCPQCSGRSFQYYGVGTQKLEDEIAVAFPN
                     VTYARIDLDSVKQGSNLPRILNDFRKGKIKILLGTQMIAKSFDFPHVALMGILSADSY
                     LEFPDFRSREKTFALLLQASGRAGRGKFPGEVIIQHSEVYKQFIENISEDRVSEFLDC
                     ELESRRELQFPPHKHLIIMHLKSASKLNGETAVNALDAVFRQHEKHYSNVFDLLGPAE
                     SPLFKVRNNFRWQFLMRTKSVFKALEIIDYILDLDYTKKILSRMTISVDVDPMDML"
     gene            1256..1939
                     /locus_tag="ENO07_04525"
     CDS             1256..1939
                     /locus_tag="ENO07_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_430537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDS01265.1"
                     /translation="MEKNKVATSLALQDIKDDPMVRALIFKADENLGVMGYTEHGDRH
                     CSLSANIAYNILKRLGYPERKAELAAIAAYMHDMGNVVNRDYHAQTAALLAYDVLSKK
                     GMSSDEIAEIMAAIGNHDEKDGSPVSAVSAAVILADKTDVHFSRVRTTDLIKQDIHDR
                     VNYAAKSSFLNVDAEKKRAMLQIKIDNTVSSVMEYFEIFLSRMVVCRRAAEYLGLKFE
                     VEINGYKLL"
     gene            1936..2979
                     /locus_tag="ENO07_04530"
     CDS             1936..2979
                     /locus_tag="ENO07_04530"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDS01266.1"
                     /translation="MNRKKSFTYASAGVDIDKADAAKSKIKALIESTFNENVVGGFGH
                     FGGCYSGKFEGFDDPITVSSSDGVGTKIKIACMMKKFDTVGIDIVHHCVNDIAVMGAH
                     PQFFLDYIGIGKLEPGTIEEIIKGLVAGCKNANMALIAGETAEMPDLYNPGEFDLAGF
                     IVGVVDRNKIIDGSKIEPGDIVLGFKSNGLHTNGYTLARKIVFEHMGWAPDKHFDELG
                     MSIGDALLVPHKLYLPVIEKLREQSHVKGFAHVTGGGIVGNLKRIFREGLTFEIDAGA
                     WEIPPIFRILQEAGNVEPEEMFRAFNMGIGLVCVVSEDSAGKVEDSFGDSENKPIRIG
                     HVMKGERPRLNLN"
     gene            3004..>3831
                     /locus_tag="ENO07_04535"
     CDS             3004..>3831
                     /locus_tag="ENO07_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01267.1"
                     /translation="MLKKSDHKNIIVRIPLKIANFFKSLILNARRRLILISALLFILA
                     YPPVPLGFLGYFCLVPMIAVTSAAGFKKGFKAGYLFGVIQGAILLYWVGVGIRSYAAS
                     AELENVFIEIFLNWIAPIGAPIALSLIHALFLALAFGIYGWISKGGNGYLFAFPFVWI
                     SVEYIRTLSEFAFPWVQLAYTQSMYVPMIQTASWWGDLGVGFWIALINVLLYLAWKYR
                     KSAGLAIGFIVPVLVLIAVNLIYGSTVRYEPVGEKIDVALIQGNITLAEKFSDGSLMF
                     "
BASE COUNT          991 a          887 c          959 g          994 t
ORIGIN      
        1 cggtaaatca gtgtcatgcc ctgtggttat gggaagtgcg accccctcgg tggaaagctg
       61 gcataatgtc aaaagcggca agtacaagct gttgcgtctg ggatcacgct gggatagccg
      121 tactcttccg aaaatacagc cggtggaatt cgctttttct cctgccggaa gctccatctc
      181 cgatgagctt cttcaaaaga tgaacgatgt tttaaagaaa gacggccagg tcatgctgct
      241 gttgaatcgt cgcgggtttg ctcccacggt caaatgttcc gactgcggcc acagcctgaa
      301 atgtccaaac tgttctgtcg gcctggtata tcatcggagt tccaattcgc tcaagtgcca
      361 tttttgcgac taccaatcaa ggactaccga tacctgcccg caatgctcgg ggagatcgtt
      421 tcaatattat ggcgtcggga cacagaagct ggaggatgaa attgctgtcg cctttcccaa
      481 tgtcacttac gccagaattg acctcgattc ggtaaagcag ggaagtaacc tgcccagaat
      541 cctgaatgac ttccggaagg gaaaaatcaa aatcctcctt ggcacgcaga tgattgccaa
      601 atcgtttgat tttccgcatg tcgcattgat gggaatactc tcagccgatt catacctgga
      661 atttcctgat tttaggagcc gggaaaagac ctttgcgctg ctcctgcagg cctcgggccg
      721 ggctggaagg gggaaattcc cgggcgaggt aattatccag cattcggaag tctacaaaca
      781 gtttatagaa aatatctccg aggaccgtgt cagtgaattt ctggactgcg aactcgaatc
      841 ccgccgggaa cttcaattcc ctccccacaa acacctgata ataatgcatc ttaaatcagc
      901 cagcaagctt aatggagaaa cggcggtaaa tgctctggat gccgtattcc gtcagcatga
      961 gaagcactat tcgaatgttt tcgatcttct ggggcccgcc gaatccccgc ttttcaaggt
     1021 tcgcaataac ttccgctggc agttcctgat gcgcacaaaa tcggttttca aggcgctgga
     1081 aataatagac tatatactcg atctggatta caccaaaaaa atcctctccc gcatgacaat
     1141 ttcggttgat gtcgacccga tggatatgct ctgaattcga gctgaatgcg gatttgcagt
     1201 gaaaataaaa gcttgaccgt gagaactgaa aagattagct tgatggccta tgaaaatgga
     1261 gaaaaataaa gtcgcaacgt cacttgcgct tcaggacatc aaggacgatc ctatggtcag
     1321 ggctttgatt ttcaaagccg atgagaacct tggagttatg ggctataccg agcatggcga
     1381 ccggcattgc agcctctccg ccaatatcgc ctataacata ctcaaacgcc tgggctaccc
     1441 ggagagaaaa gcagaacttg cggctattgc agcctacatg cacgacatgg ggaatgttgt
     1501 taatcgtgac tatcatgccc agactgcggc tcttcttgct tatgatgtcc tctcgaagaa
     1561 gggcatgtcc tccgatgaaa tcgccgaaat tatggccgcg attggaaacc atgacgaaaa
     1621 agacggttct cctgtttccg ccgtatctgc cgcggtaata ttggcggata agaccgatgt
     1681 gcatttttcg cgtgtcagaa ccactgatct gatcaagcag gatatccatg accgcgtcaa
     1741 ctatgccgcc aaatcatcat ttttgaacgt agatgcagag aaaaaacgcg ccatgctgca
     1801 gataaaaatc gataatacag tttcctccgt gatggaatat tttgaaatct tcctgagccg
     1861 catggtggta tgcaggcggg cggcagaata tctcggttta aagtttgagg ttgaaataaa
     1921 cggatataag ctcctatgaa ccgcaagaag tcatttacat acgcctccgc aggagtggat
     1981 atcgataaag ccgatgccgc caagtcaaag atcaaagccc tgatagaatc gacgttcaat
     2041 gaaaatgttg tgggcggatt cggccatttc ggcggatgct attccggcaa attcgagggt
     2101 tttgatgacc ccataacagt ctcctcatct gacggcgtcg gcacaaagat caagatcgcc
     2161 tgcatgatga aaaaattcga cactgtcggg atagatatag tccatcattg tgtcaacgat
     2221 atcgccgtca tgggcgctca tccgcaattt tttctggact acatcggaat tggtaagctg
     2281 gaaccgggca ctatcgagga aatcatcaag ggcctggtag ccggatgcaa aaacgcaaac
     2341 atggccttga tcgccggcga gacagccgaa atgccagacc tctataaccc gggtgaattt
     2401 gatctggccg gatttatagt gggagtggtt gaccgaaata aaattatcga tggctcgaag
     2461 atcgaaccgg gcgatattgt actcggattt aaatccaatg gtctgcatac caacggttat
     2521 accctggctc gcaagatcgt gttcgaacat atgggctggg caccggacaa gcattttgat
     2581 gaactcggga tgtcaatcgg cgacgccctg ctggttcccc ataagctcta tcttcctgta
     2641 atcgagaaac ttcgagagca aagccatgtc aagggattcg ctcatgttac aggcggcgga
     2701 atagttggaa atctgaagcg gatttttagg gagggcctga cctttgagat cgatgctgga
     2761 gcatgggaaa tcccgcccat attcagaatc ctgcaggaag ccggaaatgt tgagcctgaa
     2821 gaaatgtttc gggcctttaa tatgggtata ggactggtgt gtgtagtttc agaggattca
     2881 gccggaaagg tcgaagatag tttcggtgat tctgagaata agccgatcag aatcggccat
     2941 gtaatgaagg gcgaacggcc gcgattaaat ttgaattaat aaatcttccg ggataaatcc
     3001 aatttgctca agaaatccga tcataaaaat atcattgtgc gaatacctct caagatcgca
     3061 aatttcttta aatctttaat tcttaacgcc aggcgcaggc tgatcctcat atccgcctta
     3121 ttattcattc tggcttatcc gccggttccg ctgggatttt tgggttattt ctgtcttgtg
     3181 ccgatgatcg cggtgacctc tgcagcggga tttaaaaaag gcttcaaggc tggatattta
     3241 tttggtgtaa tccagggcgc tatcctgctt tactgggtcg gggtggggat tcggtcatat
     3301 gcggcttcgg ccgagctgga aaacgtgttt attgaaatct tcctcaactg gattgctccc
     3361 atcggcgcac cgattgcact ttccttgatt catgccctct ttctggctct tgccttcggc
     3421 atatacggat ggatttcgaa aggcggaaat ggataccttt tcgcctttcc ctttgtgtgg
     3481 atatcagttg aatacatccg cactctctcc gaatttgcgt ttccatgggt acagttggcc
     3541 tacactcagt cgatgtacgt tccaatgatt caaacagcct catggtgggg cgatctcggc
     3601 gtgggcttct ggattgcgct cattaatgtg ctcctctatc tggcctggaa ataccggaag
     3661 tcagccgggc ttgctatcgg ctttatagtg ccagttttag ttcttatcgc ggtaaacctg
     3721 atctatggca gcacggttag atatgaaccg gtgggagaga aaatcgatgt agccctgatt
     3781 cagggtaata ttactctggc cgaaaaattc agtgacggaa gtctcatgtt c
//
LOCUS       DSEB01000254           14888 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000117, whole genome shotgun sequence.
ACCESSION   DSEB01000254 DSEB01000000
VERSION     DSEB01000254.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14888)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14888)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14888
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000117"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..388
                     /locus_tag="ENO07_04540"
     CDS             <1..388
                     /locus_tag="ENO07_04540"
                     /inference="COORDINATES: protein motif:HMM:PF14361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01268.1"
                     /translation="TETILNGLIENVKAVEMMESMDNFVRVRSVQDFTPSQAVNFMYL
                     LKDILKEACAEKLEEPGIIRELIDFEIKIDRLTFLAFDVYVNCREQINRIRLKEVRDR
                     SAVLFEKSQRKSDEAENNNNPNGGVG"
     gene            416..1414
                     /locus_tag="ENO07_04545"
     CDS             416..1414
                     /locus_tag="ENO07_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinol oxidoreductase"
                     /protein_id="HDS01269.1"
                     /translation="MSALFALLMIIVLLLIPIIGTEVLGLNYVFGVVIPYAAIAMFLI
                     GFVYRIVRWASSAVPFKITTTCGQQKSHPWIKSSSLEAPHNTWGVIGRMALEVLFFRS
                     LFRNTKADLREGPKLVYGPSKWLWAAGLVFHWSFLIILLRHFRFFAEPAPFFVSGLQA
                     LDGFFQIGLPIIYLTNVAIVAAVTFLFLRRLYDPKLRYISLGSDYFALFLILSIAASG
                     ILMRYTGFRADIVDVKQLAMGLVSFHPVVPETIGVMFYIHLFFVAVLFGYFPFSKLMH
                     MGGIFMSPTRNMANNNREERHVNPWNYPVKTHTYEEWEDEFRDVMKAAGMPLEKEK"
     gene            1417..3069
                     /locus_tag="ENO07_04550"
     CDS             1417..3069
                     /locus_tag="ENO07_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDS01270.1"
                     /translation="MAEKIISPEELAGGIDYKPKKKTWFDPTVEFRRGTWNYAAPAKN
                     LEYLDLPNPRKWSPTDEDWKLPENWKEIILEGLRVRIKRFRSLQLFMDICVRCGACAD
                     KCHFFIGSGDPKNMPVLRAELLRSVYRKDFTMAGKLMGKMVGARELTVDVLKEWFYYF
                     YQCTECRRCSVFCPYGIDTAEITMIGRELMNLVGINIEWICTPVANCFRTGNHLGIQP
                     HGFADSIEFAVDELEEITGIRVDAPINKKGAEILFVAPSADYFASPHYYTLLGYLALF
                     HEIGLDYTWSAYASEGGNFGLFHSHEMMKRLNAKIYAEAKRLGVKWILGGECGHMWRV
                     IHQYMDTLNGPADFLEEPVSPITATKFENARNTKMVHIMEFTSDLIRNNKLKLDKSRN
                     DHWHPTFHDSCNPARAMGLIEEPRYVIRACCNNFTEMPENTIKEQTFCCGSGAGLGTD
                     ENLEMRLRGGFPRANATKFVHERDGVNILLCICAIDKATLPPLLEYWVPGVEVGGVHE
                     MLGNALVMKGGKPRTTDLRGEPLVGVAQAEEEKKEQEVSEDA"
     gene            3062..3448
                     /locus_tag="ENO07_04555"
     CDS             3062..3448
                     /locus_tag="ENO07_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01271.1"
                     /translation="MHNTGKIIIGLLIFLVIAAFPIWYAVAGGKTGEMPVLEKAARGE
                     NCIIDSVLMKETHMFLLDDWRDEVVREDDRWYRSMTLDSTIEKSLTRTCLGCHASKAN
                     FCDRCHDYLAVVPYCWDCHVDPKEFE"
     gene            3448..4218
                     /locus_tag="ENO07_04560"
     CDS             3448..4218
                     /locus_tag="ENO07_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDS01272.1"
                     /translation="MGIDRRGFMKVSGLTILGIAGGASIDGLASPQTPRKKAAEAGTR
                     RYAMVVDLKKCHSQEGCRDCVQACHLYHNVPDFDNRKDEIKWIWKDSFHHAFHSMEHE
                     HLEESITEGPALLLCNHCDNPPCTRVCPTKATWKREEDGIVMMDWHRCIGCRYCVVAC
                     PYGSRSFNWRDPRPHIKEVNPDFPTRTRGVVEKCTFCELRLAKGQKPACVEACKEGAL
                     IFGDLEDPESEVRKALQDRFSIRRKPELGTEPEVYYLV"
     gene            4226..5425
                     /locus_tag="ENO07_04565"
     CDS             4226..5425
                     /locus_tag="ENO07_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinol oxidoreductase"
                     /protein_id="HDS01273.1"
                     /translation="MIENALRGSKRYWTWVFFLLAVIFVGFLFYLKQYHEGLGITGLS
                     RDVTWGFYIAQLTFLVGVAASAVMVVLPYYLHNYKAFGKITILGEFLAISSVIMCMLF
                     VVVDIGQPSRMFNLFLHPSPNSMLFWDTVVLSGYLILNIVISIVTLGAERKGIAPPGW
                     IRPVIVFSIPWAVSIHTVTAFLYAGLGARPFWMTAILAPRFLASAFAAGPALLILLVL
                     LMRKITKFHVGDEPIKKLTVIVTYAMIINVFFVLMEFFTAFYSAMPEHVAHFKYMYFG
                     LDGNANLVPWMWTSVVLSIFALVMLIVPKFRYNFKLLPVTCVAVFIGLWIDKGIGLII
                     TGFVPSVLGEVVSYKPTFPEIMITIAIYAVGALLVTVFYKVALTVRGEVTYDRVRPLE
                     TKAVGTK"
     gene            5506..7182
                     /locus_tag="ENO07_04570"
     CDS             5506..7182
                     /locus_tag="ENO07_04570"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00581.18,HMM:PF07992.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HDS01274.1"
                     /translation="MEKSDVVIIGGVACGCKAAATLARRKPDLKITLFQKEKDLSYAT
                     CGLPYFASGDVDSFRALTETSYNVVRDVDYFRKSKGFNAVTESEVIEINRKEKKVKVK
                     DLRTGDSRDHGYDKLVIATGATPTKLPVDIPKTDRITPFTRPDDARNFRKLAQTGKIG
                     SALVVGGGFIGCEMAEATASLWGIETTLVEMESQLLPYALDEDMAIPVAKEMEANDVK
                     LYLNTAVTDFELDDDKVKVILSNDKQLQVDYVFLCVGVDPASHLAEAVGLELGQSGGI
                     KVNEYMQTSDPEIYAGGDVVELTHQITGNPIYIPLGSLANRHGRIIAEHIAGGEEKFP
                     GVLGAFMVKVFDLNVGSVGLNQQAADEAGMNANSVWGSFVDKPDYYPESKPISLKMVF
                     NPEDHSLLGIQAVGKGDVVRRIDVFSSLLQKKARIDDLLCFEHGYAPPYSEAIDPLYH
                     LGAIAKSLEHGLEQESPGGDYSEGDFILLDVREPEEVEAIPFKAKNIVHIPLNDLADN
                     LQNLEKSKRIVILCHRGMRSHQASHILRTAGFEHISYIAGGTTLAVKSLA"
     gene            7232..8464
                     /locus_tag="ENO07_04575"
     CDS             7232..8464
                     /locus_tag="ENO07_04575"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:PRK00045.2-2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDS01275.1"
                     /translation="MTRKCIDCDNKESWYLAVCGLNHRTATIEERAPLALGHDEIPSA
                     HLELANFENVYEAVAISTCNRVEFYLVLKAGVEPFNVVKNFYRNFRKLDISYLEPKCY
                     VKKELLAVEHLFKVAGGLDSMVLGESQIFGQIKESYSSACMLKTAGKIIHRLFHLSFR
                     TGKLVRSQTSLTEGASSVSGAAVSLLKNQINGNKEIPILFIGVNQMIHIAATALHKSG
                     YSNFMFANRTESKALEMAEKYGGSGHAMSELDKLLKSSEVVITCTGSPEPILDESNLL
                     PAVENSNGRKMIIMDMAIPRDTVPLPDHRDKIKIFDLEDIDFYLKETQKSREDAIPQA
                     EEIINRKLAEFGYWYEHAKSEPLVMRVEHELERIRREELAEITRELDDDTTKKIEEFS
                     KRLIDRLLTTNRRCKKGH"
     gene            8401..9129
                     /locus_tag="ENO07_04580"
     CDS             8401..9129
                     /locus_tag="ENO07_04580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HDS01276.1"
                     /translation="MFKTPDRSFANYKSTLQERTLETVKHSIEEYLPVNLALEDKNFL
                     VVGGGKVAARKIDLLVPRGTHLTVVAREPDQKIRELEKNSLLILREKEYETSDLENMD
                     YVIAATDDSKLNEEIYEECKKRNILINVVDDPGHCDFIFPSILRRGPITFTVSTAGKS
                     PFLSAFLRQIMENAFTEEWVFIGELAGKYRKYVLRKFKDREDLKAECFKRFLDVNWME
                     ILRQHDEHAAEEYYNKLLDSLEEI"
     gene            9133..10671
                     /gene="cobA"
                     /locus_tag="ENO07_04585"
     CDS             9133..10671
                     /gene="cobA"
                     /locus_tag="ENO07_04585"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein
                     motif:HMM:PF02602.13,HMM:PRK06136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDS01277.1"
                     /translation="MAENSKKKGKVYLIGAGPGDPDLITIRGSSLLSSCDVIIYDNLI
                     PHELIVTAPPHIEKIYVGKSASAHTLPQEKINELLVEHARQGKNVARLKGGDPFVFGR
                     GGEEAEYLRKNDVDFEVVPGVTAGLAAAASAGIPPSHRDMSSAILLLTAHGAADHDLF
                     VDWDWVGKVKKGTVIGYMGVRQLPTVVEKLIEGGMPPGIEAAMIERCCFSTQKVVRAR
                     LEDLPAEAERQNVNPPAIFIIGEVAALSEKLEWYNRKMLSGVRIMITRPADQSRWIYQ
                     LFRNMGAEVIAYPTIKTRYNDDKEGWEKFIRSGGKDNWLFFSGENGVRYFFGYLKSKQ
                     IDLRLVGRYAIAAAGHGTLRALRDLGYQPDYAPSVSGMVKFAQQMCEDIEVHKIKLIR
                     VRGQLPHDNIAEILKKGGAEVHPLTLYKTEYADWRENEIKRLFMHPPHMIMFSSGKSV
                     DGLVNILGHDKALELAAKSKIATIGPTTSEVVRKYKMEVELEATRHSIPDLINQIVQY
                     YQKK"
     gene            10674..12329
                     /gene="hemC"
                     /locus_tag="ENO07_04590"
     CDS             10674..12329
                     /gene="hemC"
                     /locus_tag="ENO07_04590"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:PRK00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDS01278.1"
                     /translation="MLKIATRGSRLAMRQSGMIRDMLLEKAGLEAELMVVRTAGDVIQ
                     NKPLSEVKGAGFFTKEIEQALFDGRADIAVHSLKDLPIQQPPGIDVVAIAERENPSEA
                     LLTRTENIDDSRPLNLNEGLTIGTSAIRRKAQIRALRPDLKVKDLRGNVTTRLEKLRE
                     GEYDAIIIAYAGLKRVGVEIDDLELRVLKRDEFVPAPAQGALAVETRIKDYDVASRAA
                     KINHADTKSAVDAERRLLLLCGGGCHLPLGANLTRAEEGWVMTVFWSYLLPDKTEKYL
                     RAKISSEDFDQLVEESYAYIKSTEISDLLNFKGRSKDLPRKLLITRPSEKTDELGEKL
                     AAKDIELIPYPVQSLRKNFDEKEWIDIEQEFEAYEYIILTSANAVQFFKEILEDSDIA
                     PEKYSHKKIATVGVKTAEACSEAFPDSEIIVSHISTGAGLGAHLLDYRKGKALFPCAR
                     EAGHDLQRVLGDAGWHVRRFEIYESIPEKKENLPDVNPGEVAYYCFTSPLAAEYTFDK
                     IDLPENSIIISIGPRTSEKIKKSGYKVDWELPNSNLEWLWQVL"
     gene            12314..13312
                     /gene="hemB"
                     /locus_tag="ENO07_04595"
     CDS             12314..13312
                     /gene="hemB"
                     /locus_tag="ENO07_04595"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDS01279.1"
                     /translation="MAGALKDNSNIRPRRLRSAQLIRDLTAETRLNLPGMIQPYFVIP
                     GKNKIEKIESMPGIERHTADRLVKQIEKDVKLGIKNVMLFGLSDKKDSRATMAYSKQN
                     PVVKAIRKLRTEFANDVMISCDICLCAYTDHGHCGLIKDDYVDNDASLDALSRMAVVH
                     AEAGADIVAPSDMMDFRVGAIREALDSRGFINTLIMAYTAKYASAYYGPFREAADSAP
                     SFGDRKTYQMDYRNSREALKELWLDIDEGADIVMVKPALPYLDIISLFRQNTDLPVAA
                     YNVSGEFSQAKLAVKAGLADEKALVLENLTAITRAGADIILTYHLRDLLKNRWFDE"
     gene            13305..14597
                     /gene="hemL"
                     /locus_tag="ENO07_04600"
     CDS             13305..14597
                     /gene="hemL"
                     /locus_tag="ENO07_04600"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde-2,1-aminomutase"
                     /protein_id="HDS01280.1"
                     /translation="MSKIEKSEKLFEKACSLIPKGVNSPVRAFGQVGGTPIFMERGEG
                     TYIYDVDGNKYVDYCQSWGASIMGHAHPVVVEAVKKQAEKGTSFGIPTEVEVQLADIV
                     VSNFKKIDMVRFVSSGTEAVMSAVRLARGFTGKDSVLKFEGGYHGHVDYLLVEAGSGL
                     ATLGLPASAGVTEGNAQNTYNIPLDDTDKLDQLFEKHGNDIACILVEGIPANNGLLIQ
                     SREFMHHLQQKAHKHNALLVVDEVITGFRVGKGGATEMYCIDPDIVTFGKVIGGGLPV
                     GAFGAKREIMEKIAPLGPVYQAGTLSGNPLAMSSGIAVLSYLIEYDSWKYLEEIGKFW
                     EDNIREIITKNGYPMNIARKGSIFWFSFAEGDLPTQGKQITPESIKLYAKFFHAMLEQ
                     GFYFAPSGYEVGFLTISHTKEILEVTLDAMEKGLQKIM"
BASE COUNT         3788 a         3515 c         4036 g         3549 t
ORIGIN      
        1 aaccgagact atcctgaacg gcctgatcga aaatgtcaaa gccgttgaaa tgatggagtc
       61 aatggataat ttcgtgcggg tgcgttcagt gcaggatttc accccctcgc aggcggtcaa
      121 tttcatgtat ctcctgaaag acatactgaa agaagcctgt gcggagaaat tagaggagcc
      181 cgggataata agagagctga tagacttcga gatcaaaata gacagactta cttttctggc
      241 atttgatgtt tacgtgaact gccgcgagca gatcaacaga atcaggctga aggaagtaag
      301 ggatcgttct gctgtactgt tcgagaaatc tcagagaaaa agcgatgaag ctgaaaataa
      361 taataatccc aatggcggcg ttggatagat attcgttgaa gggaggtctt catagatgag
      421 cgcccttttt gcattattaa tgatcatagt tttgctcctc atacctatca ttggcacgga
      481 ggtcctggga ctgaactatg tcttcggggt tgttataccc tatgcggcca tagccatgtt
      541 cttaatcggt tttgtttacc gcattgtcag atgggcaagc tctgcagttc ctttcaagat
      601 caccaccacg tgcggacagc aaaaatccca cccctggatc aaaagctcca gcctggaagc
      661 gccgcacaac acctgggggg tgattggaag gatggctctg gaggtgctct ttttccgttc
      721 cctgttcaga aataccaaag ctgatttgag agagggaccc aaactggttt atggccccag
      781 caaatggctg tgggccgccg gacttgtatt ccactggtca tttctgataa ttctgctgcg
      841 ccatttccgt ttctttgcgg agccggcgcc gtttttcgtc agcggcctgc aggcgctgga
      901 tggctttttc cagatcggac tgcccatcat atatctgact aatgttgcga tcgtggcagc
      961 ggtcacattt cttttcctgc gcaggcttta cgatcccaaa ctcagatata tatcgcttgg
     1021 ctccgactac ttcgcgctgt tcctgattct ttcgattgcg gccagcggga ttctgatgcg
     1081 ttatacagga tttcgggcgg acatcgtcga tgtcaaacag ctcgcgatgg gtctggtcag
     1141 tttccatccg gttgtgccgg agacgatcgg tgtgatgttt tatattcacc tgttcttcgt
     1201 ggctgtcctg ttcggctatt tcccgttcag caagctcatg cacatgggcg gcatatttat
     1261 gtctcctacc cgcaatatgg ccaacaacaa ccgtgaagag agacatgtta atccctggaa
     1321 ttatcctgtc aagacacata catacgaaga gtgggaagat gagtttagag atgtaatgaa
     1381 ggcggccggt atgccgttgg agaaggaaaa atagatatgg ctgaaaaaat tatatcaccg
     1441 gaagagttag ccggcggcat cgattacaaa ccaaagaaaa agacctggtt tgatccgaca
     1501 gtcgagttca ggaggggtac ctggaactat gctgctcctg ccaagaatct ggaatatctg
     1561 gatcttccta atccaagaaa gtggtccccg actgatgaag actggaagct gccggagaac
     1621 tggaaagaaa ttatactgga gggcctgagg gtaagaatca aaaggttccg ctccctgcag
     1681 ctttttatgg atatctgcgt tcgctgcgga gcctgcgccg ataagtgcca ctttttcatc
     1741 ggctccggag atcccaagaa tatgcccgtg ctgcgtgccg agctcctgcg ttcggtctac
     1801 cgcaaagact tcaccatggc cggcaaactt atgggcaaga tggtgggtgc gcgggaactg
     1861 acggttgatg tgctcaagga atggttctac tacttctatc agtgcaccga atgccgccgc
     1921 tgttcagtgt tctgtcctta tggcatcgac acggccgaga tcaccatgat aggccgggag
     1981 ctcatgaatc tggtgggcat caatatcgaa tggatctgca cccccgtcgc caactgcttc
     2041 aggaccggca atcacctcgg tatccagccc catggtttcg cagacagtat tgaattcgca
     2101 gtcgatgagc tggaggaaat tactggaatc agggtggatg ctcccattaa caaaaaaggc
     2161 gcggaaatcc tgtttgtggc gccctcggct gattacttcg cctcaccgca ttactacaca
     2221 ctgctcggtt acctggcgtt gttccacgaa atcggcctcg attacacctg gagcgcctat
     2281 gcctctgaag gcggtaattt cgggctgttc cattcgcatg agatgatgaa acgtctcaat
     2341 gccaaaattt atgccgaggc caagcgcctg ggcgtgaagt ggattctggg cggggaatgc
     2401 ggtcatatgt ggcgcgtgat tcaccagtac atggacacgt taaatggccc ggccgatttc
     2461 ctcgaggaac cggtctcgcc cataaccgca accaaattcg agaatgccag aaataccaaa
     2521 atggttcaca tcatggagtt cacctccgac ctgatccgaa ataacaagct gaaactggac
     2581 aagtcaagaa atgaccactg gcatccgacc ttccatgatt cctgcaatcc cgcccgggcc
     2641 atgggcctga tcgaggagcc gcgttacgtt atcagggcct gctgtaacaa ttttaccgag
     2701 atgcccgaga atacgattaa ggaacagaca ttctgctgcg gttccggggc cggtctcggt
     2761 actgatgaaa atctggagat gagattgcgg ggaggtttcc ccagggccaa tgccaccaaa
     2821 ttcgttcatg agagagacgg tgtcaatatt ctgctctgca tctgtgcgat cgataaagcc
     2881 actctcccgc cccttctgga gtactgggtt cccggcgtgg aggtcggagg agtgcatgag
     2941 atgcttggca atgcccttgt aatgaagggc ggaaagccga ggaccaccga cctgcgggga
     3001 gaaccgctgg tcggagttgc gcaggcagag gaagagaaga aggaacagga ggtgagcgag
     3061 gatgcataat accgggaaaa ttataattgg tcttctgatc ttcctcgtga ttgccgcctt
     3121 tccgatctgg tatgcggtag cgggcggtaa gacgggggag atgcccgtgc tggagaaggc
     3181 cgccagaggc gaaaactgca taatcgacag tgttctgatg aaagaaacgc atatgtttct
     3241 tctggatgac tggcgcgatg aagttgtgcg tgaagatgac cgatggtacc ggtcgatgac
     3301 ccttgacagc acgatcgaga agagcctaac ccgaacatgc ctgggctgcc atgccagcaa
     3361 ggccaatttc tgcgaccgct gtcatgatta tctggcagtg gtgccctatt gctgggattg
     3421 tcacgtcgat ccaaaggagt ttgagtgatg ggtatagata gaagaggatt catgaaggta
     3481 tccggcctga ccatactggg gatagccggg ggcgcctcta tcgatgggct ggccagcccc
     3541 caaactcccc ggaaaaaagc cgctgaagcc ggaaccagga gatatgctat ggtagtcgat
     3601 ttgaaaaagt gccatagcca ggaaggctgc agggactgtg tccaggcctg ccacctgtat
     3661 cacaatgttc ccgatttcga taatcgtaag gacgaaatca agtggatctg gaaagattca
     3721 tttcatcatg ccttccacag catggaacac gagcacctgg aagagagcat caccgaggga
     3781 cccgcgctgc ttttgtgtaa tcattgcgat aatcctccct gcaccagggt ctgccccacc
     3841 aaggccacct ggaagcgtga ggaggacggg atcgtgatga tggactggca tagatgtata
     3901 ggctgccgct attgcgtagt cgcctgtccc tacggttcca ggagcttcaa ctggagagat
     3961 ccccgtccgc atataaaaga agtcaatccg gactttccga cacgcacccg gggggtagta
     4021 gagaaatgta ccttctgcga actgcgcctg gcgaaagggc agaagcccgc ctgcgtggaa
     4081 gcttgcaaag agggcgcttt gattttcggc gatctggagg atccggaatc ggaagtcagg
     4141 aaggcgcttc aggatagatt ctcaatcaga cgtaaacccg aactcgggac tgagcccgaa
     4201 gtctattatt tagtgtgagg tgcccatgat agagaatgca ttaagaggaa gtaagagata
     4261 ctggacctgg gtttttttcc tgctcgcggt aatattcgtc gggtttctgt tttacctcaa
     4321 gcagtatcat gagggtttgg gaataacggg tctgtcgcgt gatgttacct ggggattcta
     4381 tatcgcccag ttgacattcc tggtaggtgt ggcggcctcg gctgttatgg tcgtactgcc
     4441 ctactatctg cataattata aggccttcgg caagattacg atcctggggg aattcctggc
     4501 tatcagttct gttatcatgt gcatgttgtt tgtggtggtg gatatcggtc agccctccag
     4561 gatgttcaac ctttttctgc accccagtcc caactccatg ctgttctggg atacagtggt
     4621 gctgtcggga tatcttattc tgaatattgt tatcagtatc gtaaccctgg gcgctgaacg
     4681 caaaggcatc gcgcctcccg ggtggatcag gccggtgatt gtcttttcca tcccctgggc
     4741 cgtttcgatc catactgtca cggcgttttt gtacgccggt ctgggagcga ggccgttctg
     4801 gatgaccgct atcctggcgc cccgatttct ggcctcggcc tttgcggccg gtcctgcgct
     4861 cttgattctg ctggtcctgc tcatgagaaa aattacgaaa ttccatgtgg gtgacgaacc
     4921 catcaagaag ctcaccgtga tcgtcaccta cgccatgatc atcaatgtct tcttcgtgct
     4981 gatggagttc ttcaccgcat tctattccgc catgcctgag catgtcgccc atttcaaata
     5041 catgtatttt gggcttgatg gcaatgccaa cctggtgccc tggatgtgga cctcggtcgt
     5101 gctttcgatc ttcgccctgg tgatgctgat tgtgccgaag ttccgctata atttcaagct
     5161 tctgcccgtg acctgcgtgg cggtatttat cggcctctgg atcgacaagg gcatcggcct
     5221 gatcataacc ggctttgtgc cctcggtatt gggagaggtt gtatcttata aaccgacctt
     5281 cccggagatt atgatcacta tagcgattta cgcggtgggg gcgctgctgg tcactgtgtt
     5341 ctacaaagtt gcgctgactg ttcggggcga ggtaacctac gacagggtgc gcccgctcga
     5401 aacgaaggcg gttggaacga agtagggaga ttttataaaa tagattctaa gtgccggaca
     5461 agaactcatc ttctccggca ttgtttttaa aaaaggatat ggacaatgga aaaatcggat
     5521 gttgttataa ttggcggggt ggcttgcgga tgtaaggctg cagccacact ggctagacga
     5581 aaaccggact tgaaaattac gctctttcaa aaagaaaagg acctttccta tgccacctgc
     5641 gggctgccct actttgcatc aggtgatgtc gattcattca gggcactgac ggagacttcc
     5701 tataatgtcg tccgcgacgt tgattacttc cgaaaaagca aaggctttaa tgccgttaca
     5761 gaatctgaag taattgaaat caatcgcaaa gagaaaaagg tgaaggtgaa ggacttgaga
     5821 accggagaca gccgcgatca cggctacgac aagctggtaa tagccaccgg cgccactccc
     5881 acaaaattgc ccgtagatat cccgaaaaca gaccggatta ctccgtttac ccgcccggat
     5941 gatgcccgga attttcgcaa gctggcgcaa accggtaaaa tcggcagcgc actcgtcgta
     6001 ggaggcggat tcatcggctg cgagatggcg gaggcaaccg ccagcctgtg gggcatcgag
     6061 accaccctgg tggaaatgga atcacagctt ctgccctatg cattggatga ggatatggcc
     6121 attccggtgg cgaaggagat ggaggctaat gatgtcaagc tttatctaaa taccgccgtg
     6181 actgattttg agctcgatga tgataaggtg aaggtcattc tttcaaatga taagcagctt
     6241 caagttgatt atgtgttctt gtgtgtggga gtcgatccgg cctcacatct ggcggaagca
     6301 gtcggtctgg aactgggcca gagcggagga atcaaagtaa atgaatatat gcagacctcc
     6361 gatccggaaa tctacgccgg gggggatgtt gtcgagctga cccatcaaat caccggtaat
     6421 ccgatatata ttcctttagg ctcgctggcc aatcgccacg gacggattat cgccgagcat
     6481 atcgccggag gagaggaaaa atttccaggt gttttgggcg cctttatggt caaagttttt
     6541 gacctcaatg tcggttcggt tgggctgaat caacaggccg ccgatgaggc cgggatgaat
     6601 gccaactcgg tatggggcag ctttgtcgat aaaccggatt attaccccga aagcaaaccg
     6661 atttccctga aaatggtatt taatccggaa gatcattccc tgctcgggat tcaggctgtg
     6721 ggcaagggtg atgttgtacg tcgaatcgat gtcttctcct ctcttttgca gaagaaagcc
     6781 aggatcgatg atctgctctg tttcgagcat ggttacgccc cgccctattc ggaagcgatt
     6841 gatccccttt atcatctggg agctatcgcc aaatcgctgg agcacggatt ggagcaggaa
     6901 agtcccgggg gcgactattc cgagggagat ttcatccttc tcgacgtgcg tgagccggag
     6961 gaggtggagg cgattccgtt caaagctaag aatattgtac atataccttt gaatgacctt
     7021 gcggataacc tccagaacct ggaaaagtca aaacgaattg tgatcctgtg ccatcggggc
     7081 atgcgctcgc atcaagcctc ccacattctc aggactgccg gttttgagca tatttcgtat
     7141 atcgccggag gtaccacatt ggcggtaaaa tcattagctt gacagccgct tttattcgta
     7201 tattttagaa cgtcgataaa aatggtatat tatgactcga aagtgtatag attgcgacaa
     7261 taaagaaagc tggtatctgg cagtttgcgg cctcaatcat cgcactgcga cgatcgagga
     7321 acgggcgccc ctggccctgg ggcatgacga gattccaagc gcccatctgg agctggccaa
     7381 ctttgagaat gtctacgagg cggtggcgat ctcgacctgc aaccgggtgg aattctatct
     7441 tgtgctgaaa gccggggtgg agccttttaa tgtggtcaag aatttctacc gcaatttccg
     7501 caagctggat atctcatatc tggaaccgaa atgctatgtc aaaaaggagc ttctggcagt
     7561 cgagcacctg ttcaaggtcg cggggggcct ggattccatg gttctgggcg aatcgcagat
     7621 cttcggccag atcaaggaat cgtacagctc ggcctgcatg ctcaaaaccg ccggcaagat
     7681 tatccaccgc ctgttccacc tcagcttcag gaccggaaaa ctggtccgca gccagaccag
     7741 cctgaccgaa ggggcaagct ctgtttcggg agcggcagtc agcctcctga aaaaccagat
     7801 taacggcaat aaggagatac ccatactctt tatcggcgta aatcagatga ttcacatcgc
     7861 cgccaccgcc ctgcataaat ccggctacag caatttcatg ttcgccaacc gcaccgaatc
     7921 gaaagcgctc gagatggccg aaaaatacgg cgggtccgga cacgccatgt ccgaacttga
     7981 caaactcctt aaatcctctg aggtggtgat tacatgcacc ggttctccgg aaccgattct
     8041 ggatgagtcc aatctcctcc cggcagtgga aaacagcaat ggccggaaaa tgataattat
     8101 ggacatggcc attccaagag atacagttcc gcttcccgat cacagggaca agattaagat
     8161 cttcgacctc gaagatattg atttctattt gaaagagacc cagaaaagca gggaagacgc
     8221 tatcccgcag gccgaggaga taatcaaccg caagctcgcc gaattcgggt actggtacga
     8281 acatgccaaa agcgagccgc tggtcatgcg cgtagagcat gagctggaaa gaattcgacg
     8341 cgaggagttg gccgagatta ccagagagct ggatgatgat actacaaaga agatcgagga
     8401 attttcaaaa cgcctgatag atcgtttgct aactacaaat cgacgctgca agaaaggaca
     8461 ttagaaactg tgaaacattc tattgaggaa taccttccgg taaaccttgc ccttgaagat
     8521 aaaaattttc tggtggtggg gggcggcaag gttgccgcac gcaaaatcga cctgcttgtt
     8581 ccacgcggca cccacctgac tgtggtcgcc agggaaccgg atcaaaagat acgcgaactg
     8641 gaaaagaact cgctgctgat tttgcgagaa aaagaatatg agacctctga cctggaaaac
     8701 atggattatg ttatcgcggc gactgatgac agcaaattaa atgaagaaat ttatgaagaa
     8761 tgcaaaaaac gcaatatcct gataaatgtt gttgatgacc ccgggcactg tgacttcatc
     8821 tttccttcaa tattgagacg cggtccgata accttcacgg tttcaacggc cggcaagtcg
     8881 ccctttctct cagcattctt gaggcagatc atggaaaatg cttttaccga ggaatgggtc
     8941 tttatagggg aactggccgg taaataccgc aagtatgtcc tgcgcaagtt caaggatcgt
     9001 gaggacctca aagctgaatg cttcaagcgt tttctggatg tcaactggat ggaaatactg
     9061 cgccagcatg atgaacatgc ggccgaagaa tattataata aactgctgga cagtctggaa
     9121 gaaatttaat tgatggctga aaacagcaaa aagaagggca aagtttatct catcggcgcg
     9181 gggccgggcg atccggacct gattacaatc aggggtagca gcctgctcag ctcctgcgat
     9241 gtaattatct atgacaacct gatccctcat gaattgatcg tgacggcgcc tccgcacata
     9301 gaaaaaattt acgtgggaaa atctgccagc gcacacactc ttccccagga aaaaatcaat
     9361 gagctgctgg ttgaacatgc cagacagggc aaaaatgtcg cccgtttaaa aggaggcgat
     9421 ccgtttgtct tcggaagagg cggggaagaa gcggaatatc tcaggaaaaa tgacgtcgat
     9481 ttcgaagttg tccccggagt taccgccggg cttgccgccg cggcctcagc cggaattccc
     9541 cccagccaca gggatatgtc cagcgccata cttttgctca ccgcgcacgg cgccgccgat
     9601 catgatttat tcgtggattg ggattgggtc ggaaaagtca agaagggtac tgtcatcgga
     9661 tacatgggcg taagacagct tccaaccgtg gttgaaaaat tgatcgaggg tgggatgcct
     9721 cccggaatcg aggcggctat gatcgaacgc tgctgcttct caactcagaa ggtggtgcgg
     9781 gcccgcctgg aggatttacc cgcggaagcg gaaagacaga atgttaatcc tccggcaata
     9841 ttcattattg gcgaggtggc cgcattgtcc gaaaagctgg aatggtataa tcgcaagatg
     9901 ctttccggcg tacgcattat gatcacccgt ccggccgacc agtccagatg gatttaccag
     9961 ctcttccgca atatgggcgc ggaggtgatc gcatacccca ccatcaaaac cagatataat
    10021 gatgataaag agggctggga gaaatttatt agatccggcg gcaaagataa ctggcttttc
    10081 ttttccggtg agaacggcgt gcgctacttc ttcggctacc tgaaatcaaa acagatcgat
    10141 ctgcgtctgg tgggacgtta tgctatagca gcggcggggc atggcaccct gcgggctttg
    10201 agagatctcg gctatcagcc ggattatgct ccctctgttt ccggtatggt gaaattcgca
    10261 cagcaaatgt gcgaggatat cgaagtacat aagataaagc tgatcagggt gcgcggtcag
    10321 ctgccgcatg ataatattgc ggaaattctg aaaaagggcg gagcagaggt ccatcccctg
    10381 acgttatata agaccgaata tgcagactgg cgggagaatg aaatcaaaag gcttttcatg
    10441 catcctcccc acatgataat gttctcgagc gggaaatcgg ttgacggtct ggtgaatatt
    10501 ctcgggcatg ataaggccct ggaacttgcc gcaaaatcga aaatagcgac tattggcccg
    10561 accacatcgg aagtggtcag gaaatacaaa atggaggtcg aactggaggc aaccaggcat
    10621 tcaatacctg acttgataaa tcagatagtt caatattatc aaaagaagta accatgttga
    10681 agatagcgac aaggggaagc aggctggcca tgcgccagtc gggaatgata cgggatatgc
    10741 ttttggagaa ggccggtctg gaagcggagc ttatggtggt caggactgcg ggtgatgtta
    10801 tccagaataa accgctctca gaggtcaaag gtgcgggctt cttcaccaaa gagatagagc
    10861 aggctctttt tgacggccgc gctgatattg ccgtgcactc tcttaaggac ttaccgatac
    10921 agcaacctcc cggaatcgat gtcgtcgcta tcgccgaaag agaaaatccc tcggaggcgc
    10981 tgcttaccag gactgagaat attgatgatt cccggccctt gaacctgaat gaggggttaa
    11041 ctatcggtac cagcgcaatc aggcggaagg cccagatcag ggcgctgcgg ccggatctga
    11101 aggtcaagga tcttcgcgga aacgtgacca cgcgcctgga gaaactgcgg gagggcgaat
    11161 atgatgcgat aattatcgct tacgccgggc tcaagcgagt cggggtggag attgacgatc
    11221 tggagctgcg ggtgctgaag agagatgaat tcgtacctgc cccggctcag ggtgcattgg
    11281 cggttgaaac acggatcaaa gattatgatg tggcaagcag ggcggcaaag ataaatcatg
    11341 ctgataccaa atcggccgtt gatgccgaga gaagacttct gctcctgtgc ggcggcggat
    11401 gtcatctccc cctgggagcg aatctcacca gagcagaaga aggctgggtg atgacggtct
    11461 tctggtccta cctgcttccc gataaaaccg aaaaatatct tcgtgctaag ataagctcgg
    11521 aagactttga tcaactggtt gaagaaagtt acgcatacat taaatcgact gagatttctg
    11581 atcttttgaa ttttaagggg cgctccaaag atctcccgcg gaaactcttg atcacccgtc
    11641 cctcggaaaa gacggatgag ctgggtgaaa agctcgccgc aaaggatata gagcttattc
    11701 catatccggt ccagagctta agaaaaaatt ttgatgagaa ggaatggatc gatatcgagc
    11761 aggaatttga ggcttatgaa tatataattc taaccagcgc caatgcggtc cagttcttca
    11821 aggagattct ggaggacagt gatattgctc cggaaaaata ctctcataaa aaaatcgcca
    11881 ctgtgggagt gaaaaccgcg gaggcctgca gtgaagcctt ccccgattcc gagattatcg
    11941 tttcccatat ttccactggt gcggggttgg gcgcccatct gcttgattac aggaagggca
    12001 aggccctttt cccttgcgcc cgggaagccg ggcacgatct ccaaagagtg cttggcgatg
    12061 ccggatggca tgtcaggagg ttcgaaatct atgaatcgat tcccgaaaaa aaagagaatc
    12121 ttcccgatgt caatcccgga gaagttgcat attattgttt caccagcccg ttggcggctg
    12181 aatacacatt cgataagata gatttacctg aaaatagtat cataatatca atcggcccca
    12241 ggacatcgga aaagattaag aaatcgggct ataaggttga ctgggaactt cccaattcaa
    12301 atctggagtg gttatggcag gtgctttaaa agataattcg aacattcgtc ccagaaggct
    12361 gaggtcagcg caattaatcc gtgacttgac tgcggaaacg cgccttaacc ttcccggcat
    12421 gatccagccc tattttgtta tccccggaaa aaataagatt gagaaaatag aatcgatgcc
    12481 cggtatcgag aggcatactg ccgatagact cgtcaagcaa atcgaaaagg acgtcaagct
    12541 tggtataaag aatgtcatgc tcttcggtct gagcgataag aaagactcca gggccactat
    12601 ggcatactcg aagcagaatc ccgtggtgaa agctatccgg aaattgagaa cagaatttgc
    12661 gaatgatgtg atgatatcct gcgatatctg cctttgtgct tacaccgacc atggtcattg
    12721 cgggttgatt aaggatgatt atgttgacaa cgatgcatct cttgatgctc tttccagaat
    12781 ggcggtcgtg catgccgagg cgggggccga tattgtagcg ccgtcggaca tgatggactt
    12841 tcgcgttggc gcgatcaggg aggcgctgga ttcgagggga tttattaata ccctgatcat
    12901 ggcttacacc gccaaatacg cttcggctta ttatggcccg ttccgcgagg cggcggactc
    12961 tgcaccgtca ttcggcgacc gtaaaaccta ccagatggat taccgcaaca gccgtgaggc
    13021 tttgaaggag ctgtggctgg atatcgacga gggggcggat attgtcatgg tcaagccggc
    13081 cctgccctat ctggatataa tatcactatt cagacagaat accgacctgc cggtcgcggc
    13141 ttataatgtt tccggtgaat tctcgcaggc aaagctggcg gtgaaagccg gcctcgctga
    13201 tgagaaggcg ctcgttctgg aaaatctcac cgccatcacc cgtgcaggcg ccgacataat
    13261 actgacttac catctccgtg atttattgaa gaacaggtgg ttcgatgagt aaaattgaaa
    13321 aatcggaaaa actattcgag aaagcatgca gtctcatccc caaaggtgta aattcgccgg
    13381 tgcgggcctt cggccaggtg ggcggtacgc cgatctttat ggagcgcggc gagggcacat
    13441 atatatatga tgtcgatggt aataaatatg tcgattactg ccagtcatgg ggcgcatcta
    13501 ttatgggcca tgcccatccg gtggtcgtgg aggctgtcaa aaaacaggcc gagaaaggta
    13561 ccagtttcgg aattcccacc gaggtcgagg ttcagctcgc ggatattgta gtgtccaatt
    13621 ttaagaagat cgatatggtg cgttttgtat cctccggtac cgaggctgtc atgtccgcgg
    13681 tccgtcttgc ccgaggcttc accgggaagg atagtgtctt aaaattcgag ggcgggtatc
    13741 atggtcatgt ggactatctc ctggttgaag cgggttcagg tctcgccact ctgggccttc
    13801 ccgcatccgc aggtgtgaca gaaggaaatg cgcagaacac atataatatc ccactggatg
    13861 ataccgataa gctggatcag ctttttgaaa agcacggcaa tgatatcgcc tgcattcttg
    13921 tcgagggcat tccggctaac aacggcctct tgatacagtc ccgagaattc atgcatcacc
    13981 tccagcagaa agcacataag cataatgcgc ttctggttgt cgatgaggtt atcaccggat
    14041 tccgtgtcgg caaaggcggg gcgacagaaa tgtattgcat cgatccggat atagtgacct
    14101 ttggaaaggt catcggcggc gggcttccgg taggagcatt cggagcgaaa cgggagatca
    14161 tggagaagat tgccccgctc ggaccggttt atcaggctgg gaccttgtct ggcaatccgc
    14221 ttgcaatgtc ttcaggtatt gccgtgttgt cttatctaat cgaatatgat tcatggaaat
    14281 atctggagga aattgggaaa ttctgggaag ataatataag ggaaatcata acgaagaacg
    14341 gctatcctat gaatattgcc aggaaaggtt cgatcttctg gttttcattt gccgaaggcg
    14401 atcttcctac tcaaggcaag caaatcactc cagaatcaat aaaactctat gccaagttct
    14461 tccatgccat gctggaacag ggcttctatt tcgctccctc gggctatgaa gtcggatttc
    14521 tgaccatttc gcatacaaaa gagattttgg aagtgacctt ggatgcaatg gaaaagggcc
    14581 tccaaaagat catgtagatt aaatctatgg gggattcgac atcaccccgc ccggagtggg
    14641 ttcccggcgg cactatatgt aagcggtcga agctatgtgc cggcaggtcg tagatccggc
    14701 tcttgacggg gatcctttcc tgccggctac tttcctgttg cagcaggtcc tcagacctgc
    14761 cgtccgccaa ttgcgcgatt cacaatccgc cggttctgag caactggcgg aactctgtta
    14821 gtctgaaact ccacaccata ccgctcaaaa acaatttgat ttattaggtt aaatatatat
    14881 atcttaat
//
LOCUS       DSEB01000255           15123 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000114, whole genome shotgun sequence.
ACCESSION   DSEB01000255 DSEB01000000
VERSION     DSEB01000255.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15123)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15123)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15123
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000114"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1408)
                     /locus_tag="ENO07_04605"
     CDS             complement(<1..1408)
                     /locus_tag="ENO07_04605"
                     /inference="COORDINATES: protein motif:HMM:PF00082.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01281.1"
                     /translation="MRSRILMAACLFTLFSAFPAESSNPSKIHPALEEKLAQSQGEFQ
                     EIIIRLGDRLDKQKLDQNLSLASYTRQAAHSLAVTALKFHADNTQAGLLEFLSNERNK
                     GNVRAFKSYWIDNLIWASVRPELLVELEKFDEIDLIYPEVKVSIVEPITHASPFSPGQ
                     VGVTDELKAMGADSAWKLGYTGAGTVVASFDTGIEGDHPALQGSYRGTYGFSHEQCWF
                     DPISNDTFPHIFSSIGELTRRAHGTATMGLMVGKDDVNGDTVGVAFDARWISAGVIDI
                     PNDQFNHQFYLLDAFQWAADPDGDPNTIEDVPDVLSNSWGYQNTALGCSDLFYGVIDN
                     LETLGIVVMFSAGNFGSNYRTLVNPANRATSPYNSFAVGMISDDYVDIAVDPTSSRGP
                     SNCNPEIIKPNVVAPGRNVTTTGPIAIYPGGIVTNFTGTSFSCPLAAGAALILRQAFP
                     NAPVDSIKKALMLSATDID"
     gene            complement(1488..1802)
                     /locus_tag="ENO07_04610"
     CDS             complement(1488..1802)
                     /locus_tag="ENO07_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDS01282.1"
                     /translation="MILSTTPALEGKKIEEYIGIVTGEAILGANIVRDIFASITDIVG
                     GRSGKYEKELAKARQIALKEMSDEAQSIGANAVVGVDLDYEVIRDGMLMVSASGTAVK
                     IR"
     gene            complement(1946..2725)
                     /locus_tag="ENO07_04615"
     CDS             complement(1946..2725)
                     /locus_tag="ENO07_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004400800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase"
                     /protein_id="HDS01283.1"
                     /translation="MAYNNILVEKDGHLAILTINRPKAMNALNNETLTEIDKAYDELM
                     EDGQTRVVIITGAEKAFVAGADIKELMEADGEKAKVISSKGNTLFRKIESCDLVSIAA
                     VNGFALGGGCELAMACDIRIASEKAKMGQPEVNLGLIPGYGGTQRLTRLVGKGKAKEL
                     IFTADMLDARDALKVGLVEHVTEPGELMDEAKKMAKKIISKGPIAVKLAKQAINFGSE
                     TSLPDGIAFEIEKFGQVCSTEDKNEGTKAFLEKRAPEFKAK"
     gene            complement(2732..3586)
                     /locus_tag="ENO07_04620"
     CDS             complement(2732..3586)
                     /locus_tag="ENO07_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HDS01284.1"
                     /translation="MVIKKVGIVGFGQMGTGIAQVSASAGYNVLARDIDSNYLDKGVA
                     RITKNLDKAIEKGKLKEEDKSKVLGNINTTTHLEEFKDCDLVIEAVIENMDEKVDVFM
                     ELDKIVQKDAIFASNTSSLSITDIAARTTRPEQFMGLHFFNPVHIMKLVEVVKTIATS
                     ENTFKHGKEFAESVGKTSVTCGDRPGFVVNRLLIPYLLDAVRILEDGTASREDIDNGM
                     MLGCGHPMGPLTLIDFIGLDTVLYIGDIMFEEFKGSHFAAPPLLRRMVNMGYLGRKSG
                     RGFYDYKK"
     gene            complement(3674..5716)
                     /locus_tag="ENO07_04625"
     CDS             complement(3674..5716)
                     /locus_tag="ENO07_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HDS01285.1"
                     /translation="MYRHLIYMLIALALLLSTLQARRLEPGSDDHDRYLTACEHYKKG
                     DYEEARNILYEMLRDHDNDPYLNHILGMSFAKMGGVHNRNYARKYLKQAVRLEEDSLD
                     WRYDLGLLLLESDMEAAAEEQFQELVKRDSNYVRAYYHIMDICIERFCHNGNRSKLAQ
                     GYRTALVSQGRYPGDDVIAYKKALIEMLFKSDNQAVKTISPPHRPDTLQTEIRLLDAY
                     LKYRLRDFDQSYALFSSALSDLSEDESSGYRDITLLLTNQQKREYEELPESQKAQFED
                     NFWAGLDPDLTTRVNEKIVEHFARVYFAEIMFARPEADVHGWEMDQGKLYIRYGQPLS
                     AKWYLPDDNDPLLSCRWSWVYMFDGRPVELEFLNIFGGDNYQLAPMNYGGDVKIADDL
                     VNEIPNVATFAQSSNLINSIFSYFIYKGPEGESMLDLFVATPYDQFVYEPVEGHAVCS
                     VEYRTALIDLEGAILDRKTAGQQIVISPTLSKNPDFYNFATISMKAPADSLNIACAIE
                     QKSAQRVNVYERPLRIYDFRDSGFHMSSLILASKVGEKEKDSLFNRRDIKLIPNFTNT
                     YRTDDTLIVYYEIYDLPLNIRGRSHYRMTYTIQEQDIPGNIFGMLASVLSSKQKTGIT
                     HTADRGDIRTDRFEPLKIDISSLEPGLYEFTLTIEEFVLGKTLTRSARFSVVDESG"
     gene            5980..6438
                     /gene="mscL"
                     /locus_tag="ENO07_04630"
     CDS             5980..6438
                     /gene="mscL"
                     /locus_tag="ENO07_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDS01286.1"
                     /translation="MMKEFKEFAVKGNVVDMAVGIIIGVAFGTVVSSLVADVIMPPIG
                     LLLGNVDFSNLFFVLKQGVTAGPYATVMEATEAGAVTVNYGVFINTIISFIIVAFAVF
                     MLIKNVNRMERKQPPPPPPSTKECPICFTNIPIKATRCPNCTSELQTAEG"
     gene            complement(6616..7206)
                     /locus_tag="ENO07_04635"
     CDS             complement(6616..7206)
                     /locus_tag="ENO07_04635"
                     /inference="COORDINATES: protein motif:HMM:PF02525.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDS01287.1"
                     /translation="MKVLIVYGSPDGKGNSRRLADALQEGLKTENTEVEEIDIHDYKV
                     SFVWSNYFGDALQNNFEKAGDDDMPKLKDKMFAADILILVSPVYWYQLGGRMKTFVDR
                     WADTINTDFSSDLMGKGLALVTTHSGLNIMNSSNYLQLAMEATARFMGMVWMGAVDAP
                     TSLPISSGPIDAHYQIAKNFGEKLARGENLIGQKVL"
     gene            complement(7378..8625)
                     /gene="rho"
                     /locus_tag="ENO07_04640"
     CDS             complement(7378..8625)
                     /gene="rho"
                     /locus_tag="ENO07_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDS01288.1"
                     /translation="MDIVDLKNKTIAELLKTAEELDIPGVSGLRKQELIFKILEANTE
                     KDGLIFGEGVLEILDEGYGFLRSPDYNYLPGPDDIYVSPSQIKRFDLKTGDTVSGQVR
                     PPKESERYFALLKIEAVNFENPEIAKHKILFDNLTPLYPNLKLKLEFKNDDWTTRIMD
                     LMTPIGKGQRGLIVSPPKAGKTIILQKIANAIISNQPEVKLIVLLIDERPEEVTEMAR
                     SVSGEVISSTFDEPAERHVQVADMVINKAKRLVEHGQDVVILLDSITRLARAHNAVVP
                     HSGKILSGGVDSNALHRPKRFFGAARNIEEGGSLTIIGTALIETGSRMDEVIFEEFKG
                     TGNMEIVLDRRLSDRRIFPAMDINRSGTRKEELLLSSNDLNKVWILRKFLAEMNPVEG
                     MEFLLDRIKKTKTNKAFLDSMKG"
     gene            complement(8726..9076)
                     /gene="rsfS"
                     /locus_tag="ENO07_04645"
     CDS             complement(8726..9076)
                     /gene="rsfS"
                     /locus_tag="ENO07_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDS01289.1"
                     /translation="MAAEKSYDLARKAGEFALLRKANEVQILDLRKITSITDYFVICS
                     GDADVQVRAIAETIIEKLKEEDIRVWNVEGFDTGRWILLDFVDVVVHVFLQEAREFYG
                     LERLWGDAPTESIQ"
     gene            complement(9107..9934)
                     /locus_tag="ENO07_04650"
     CDS             complement(9107..9934)
                     /locus_tag="ENO07_04650"
                     /inference="COORDINATES: protein motif:HMM:PF13399.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HDS01290.1"
                     /translation="MLRKKKSKIPRQKKYRQRIAPKTKGRLFDITLIVLSVLVVVLLS
                     STAIRLVRAETKEITPELTVLRVQIANGSGINGAAGKMAEWVEKQSSDILKYDVIDIT
                     NFENEAMSQTIVLVRDPMALDKKDMIAEQLGIPESNISMNELKNNFLALDITIVVGRD
                     YEKYESHPELILTEILNGCGIKGAANQFAMHLTQLSDESMTFEIVKTENFSNFNVNES
                     MIIVKTDKAEGISARLARKLDIKKDNIIDDRSGKEAPQSDLTIVVGHDWGKRLTASN"
     gene            complement(9958..10434)
                     /locus_tag="ENO07_04655"
     CDS             complement(9958..10434)
                     /locus_tag="ENO07_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01291.1"
                     /translation="MRHLVITTLFILLLSIPVLGQSGLERQEEATSADAIGKARIDNK
                     FSLLDLSRLDMSHSYTISYFSANGQGTTIGMYMNSIRYRISNPLTLNVNLAWVHQPGQ
                     LLGGDRGTPTDYGSIYPSFSLEYRPSDKFYLEIGYHSVPAYMYYYNTDRMRRNWFY"
     gene            complement(10508..11191)
                     /locus_tag="ENO07_04660"
     CDS             complement(10508..11191)
                     /locus_tag="ENO07_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine pyrophosphorylase"
                     /protein_id="HDS01292.1"
                     /translation="MKSDLPKVLHDLKGKPIIQYLAETLKDMGLAKVVVVVGYKRHLV
                     EEALADYEYDFAVQEKQLGTGHAVMMAEEALSDFEGDVLVLAGDVPFIRKETIKNLLD
                     LHNRKDAAATVLTSEPPDPTGYGRVIKKSQDFVEKIVEHKDCSGEELRIGEINTGTFV
                     FRKRELFENLKHIDNSNAQGEYYLTDLMEIFLKQGKKTAAFKTDDYREALGINSREQL
                     EELAKAYNL"
     gene            complement(11244..11597)
                     /locus_tag="ENO07_04665"
     CDS             complement(11244..11597)
                     /locus_tag="ENO07_04665"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011918194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDS01293.1"
                     /translation="MTVEYLKSKIHRATVTEANLEYEGSITIDKTLLEATGLLEYEKV
                     QVLNVNTGARLETYVIEGEADSGVICLNGAAARLGQKGDKLIIISYCSLSEDEAQGHK
                     PRVISVDNDNRIKSA"
     gene            complement(11594..12451)
                     /locus_tag="ENO07_04670"
     CDS             complement(11594..12451)
                     /locus_tag="ENO07_04670"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013291054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDS01294.1"
                     /translation="MKTVKSLKSMQRLAGRLKCEGKKIAFVPTMGALHEGHLALIRKA
                     KKLGDVTVVSIFVNPAQFGPSEDFSKYPRTFKEDRRRLRDLKVDYVFYPDRESTYPPD
                     YETYVNLEKMSKVLEGQFRPIHFRGVATIVTKLLNIVQPDIAVFGQKDYQQSVIIKKM
                     VRDLNLPVKIVIGKTVREESGLALSSRNKYLDEEQKKRATVLYKSLMHARDMIRNGER
                     NASKIKRSMRLMIDRMAGTKIDYIAITDDERLEQVRRLSGKFTISLAVWLDEVRLIDN
                     ISVFLGDEK"
     gene            complement(12485..13273)
                     /gene="panB"
                     /locus_tag="ENO07_04675"
     CDS             complement(12485..13273)
                     /gene="panB"
                     /locus_tag="ENO07_04675"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDS01295.1"
                     /translation="MTVPGLIKKKSEGEKIVFLTAYDYFTAKQLDAAGADGLLVGDSL
                     NMVVYGHENTLSLPLDQILYHTEAVSRGAKRAMVVGDMPFMSYQPSLEDAVRNAGRFL
                     KEAGAHAVKLEGGIEMIDVVMRIVESGIPVMGHIGLTPQSIFKFGGYRVQGKEETEKT
                     YLFESAEALQEAGCFSIVLEAVKSKIAEEITKRIEIPTIGIGAGPGCDGQIMVINDLM
                     GMDEDFYAKFVRRYFNLGQKIREVGEQYAKDVRSGDYPSKDESY"
     gene            complement(13314..13961)
                     /locus_tag="ENO07_04680"
     CDS             complement(13314..13961)
                     /locus_tag="ENO07_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDS01296.1"
                     /translation="MKSVNYIAVEGPIGVGKTSLAKLLAEKMDAHLIMEEATENPFLA
                     DFYRNKKKYAFQTQIFFLLARYQQLAKLNTHDLFHSLIVSDYTFEKDQLFAKVNLEER
                     ELILYDKIAASLSRDVPKPDLVIFLQAATETLYQRIKQRGVPFERNIDIYYLDELNEA
                     YNYYFFEYDLTPLLVVKTDEIDFVNNANDFNDLVEQIQKPLSGTHYYVPPGSLEL"
     gene            complement(13948..14478)
                     /gene="folK"
                     /locus_tag="ENO07_04685"
     CDS             complement(13948..14478)
                     /gene="folK"
                     /locus_tag="ENO07_04685"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDS01297.1"
                     /translation="MPEDRIYLGLGSNLGQRENFLKSALEKMKASDKIGVLRVSPVYE
                     TEPVGFTDQDSFLNIVVEISSKLPPEELMADLEEIESRVGKKAEFKNGPREIDIDILL
                     YGKQVHESEKLKIPHPGLSRREFALRPLLDLEPYIADPTTNMPYRAYLKRIKGEKQVS
                     IKREIDLGFRNEDEIS"
     gene            complement(14520..14876)
                     /gene="folB"
                     /locus_tag="ENO07_04690"
     CDS             complement(14520..14876)
                     /gene="folB"
                     /locus_tag="ENO07_04690"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDS01298.1"
                     /translation="MADKIKVSNMVFYAYHGTSSAERKTGNRFEVDVEVQADLSKAAK
                     SDKLADTISYTRIYHMTNELFSFKKYNLIEKVGYLLADKIMEEFSPEKVTIRIRKKIP
                     PIPGNLDHVEIEIEKP"
BASE COUNT         3540 a         3906 c         3454 g         4223 t
ORIGIN      
        1 catcaatatc cgtggccgaa agcatcaaag ccttcttgat tgaatcaacc ggggcattgg
       61 ggaaggcctg acgcagaatc agggccgcgc cggccgcgag cgggcatgaa aaggaggtgc
      121 cggtgaagtt agtgactatg ccgcctggat aaatagcgat cggcccggtt gtggttacat
      181 ttcttcccgg agccacaacg ttgggcttga ttatctccgg gttacaattt gaggggccgc
      241 gtgatgatgt cgggtcaacc gcgatatcga cataatcatc acttatcatt cccacagcaa
      301 aagaattata tgggctcgta gcgcgattgg ccggatttac aagcgtgcgg taattagaac
      361 cgaagttgcc ggccgagaac attacgacta ttccgagcgt ctctaaatta tcaatcaccc
      421 cgtaaaacag atccgaacac cccagggcgg tgttttggta tccccatgag ttcgagagaa
      481 catctggtac gtcctcaatc gtattaggat ctccatcagg atcggccgcc cactggaatg
      541 cgtccagcag atagaactgg tgattaaact gatcgttggg aatatcaatt actccggccg
      601 aaatccaccg ggcgtcaaag gccacaccta cagtatcgcc gtttacgtca tccttgccca
      661 ccatcaggcc catggtcgcg gttccatgtg ccctgcgagt aagttcgcca atactgctga
      721 atatatgcgg aaatgtgtcg ttcgatattg gatcgaacca gcactgttca tgagagaatc
      781 cgtatgttcc cctgtaactt ccctgaaggg cgggatgatc tccttcgatt cccgtgtcga
      841 agctggccac aacagtaccc gcgcccgtgt atcccagttt ccaggcgctg tccgcgccca
      901 tggctttcag ctcgtcagtt actccaacct gtcccggtga aaatggggat gcatgcgtga
      961 tcggttctac gattgaaact ttaacctccg gatatatcaa atctatttcg tcaaacttct
     1021 ctaattctac cagcagctcc ggcctgacac tggcccagat cagattatcg atccagtagc
     1081 ttttgaaagc acggacattg cccttattcc gttcattgga aagaaactcc aaaaggccag
     1141 cctgagtatt gtctgcatgg aatttcaacg cagtaaccgc cagagaatgt gccgcctggc
     1201 gtgtatatga tgccagcgac aggttctgat ccaatttctg cttatcaagt ctgtcgccca
     1261 ggcggattat gatctcctga aattcgccct ggctttgagc gagcttttct tcaagcgcag
     1321 gatgaatctt cgaggggttc gatgattccg cgggaaacgc ggaaaacaag gtaaacaaac
     1381 atgcggccat taatattctg gatctcatat tttctccctg gtggatttca gccagttaaa
     1441 caatgctaat aatataacgt ttcaaaggca ttatcgtttt gaacgggcta acgtattttt
     1501 acagcagttc ccgaagccga taccatcagc atgccgtcgc gaataacctc gtaatcgaga
     1561 tcgaccccga ccacagcatt ggcgccgata ctttgggcct cgtcggacat ctcctttaat
     1621 gcaatctgcc tcgctttggc cagttctttt tcatatttgc cggacctgcc gccgacaatg
     1681 tcggttatgg aggcgaatat atcccggaca atgttggcgc ccagaatcgc ctcgccggta
     1741 acaatgccta tatattcttc gattttcttt ccctcgagtg cgggtgttgt agataagatc
     1801 atcttagtct cctgatttta tcgatgctct aaaatagcga tttgattccg tttttcaacc
     1861 caaatttcag caaagattct gggcaaaaag agagcgatgg tatcctctga taccaccgct
     1921 caatttttaa catcggtttt gtagcttatt tggccttgaa ttccggggcg cgcttctcca
     1981 ggaaggcttt ggtgccctcg tttttatcct cggtggagca aacctgtccg aatttctcga
     2041 tttcgaatgc aattccatcg ggaagactgg tctcagaacc gaaattaata gcttgtttgg
     2101 ccagtttgac cgcaatcggg ccttttgaga tgatcttttt ggccattttt ttggcctcat
     2161 ccataagctc gcccggttcg gtcacatgct cgaccaggcc gaccttcagg gcatccctgg
     2221 catccagcat atcagcggta aaaatcagtt ctttagcctt gcctttgccc accagacggg
     2281 tcagtctttg tgtgccgccg tacccgggaa taagtcccag gtttacctcg ggctgtccca
     2341 tctttgcttt ttctgatgca atccggatgt cgcaggccat ggccagttcg catccgccgc
     2401 ccagcgcaaa accgttgacc gcggcgatag agaccaggtc gcagctttcg attttcctga
     2461 acagggtatt gccttttgat gatatcacct tggccttctc cccgtcggcc tccatcaatt
     2521 ctttgatgtc cgctcctgcc acaaaggcct tttcagcccc ggttattatt accacacgag
     2581 tctgaccatc ttccatcaat tcgtcatagg ccttatcgat ttctgtcagg gtttcattat
     2641 tcaaggcgtt cattgccttg ggacggttga tagtcaatat cgccaggtgg ccgtcctttt
     2701 ccactaaaat attattgtaa gccattttcc ctcacttttt atagtcgtaa aatcctcttc
     2761 cgctctttct gcccagatat cccatattca ccatgcgcct cagaagcggc ggagcggcga
     2821 aatgggagcc cttgaattcc tcgaacatta tgtcgccgat atagagcacc gtatccagtc
     2881 ctatgaaatc gatcagtgtc aacggtccca tgggatggcc gcagccgagc atcataccgt
     2941 tatcaatatc ctccctgctg gcggtgccat cttccagaat ccggaccgca tccagaagat
     3001 acggtatcag gagcctattg accacaaagc cgggacgatc cccgcaggtc accgaggttt
     3061 tgcctaccga ttcggcgaat tccttaccgt gcttgaaggt gttctcggat gtcgcaatcg
     3121 tcttcacgac ctccaccagc ttcatgatgt gcaccggatt gaaaaagtgc aggcccatga
     3181 actgttccgg tcgggttgtg cgggccgcta tatcagtgat cgatagtgag gaggtgttgg
     3241 aggcgaagat tgcatccttc tggactatct tatcaagctc catgaataca tcaacctttt
     3301 cgtccatatt ctcgatcacc gcctcaatca ccagatcgca gtccttgaac tcctccagat
     3361 gcgtagtggt attgatattt cccagcactt tggatttgtc ttcttccttg agcttgcctt
     3421 tttctatagc tttgtcgagg tttttagtta ttcttgcgac tcccttgtcg agataattgc
     3481 tgtcgatatc ccgcgccaga acattatatc cggccgaggc cgagacctgg gcaatcccgg
     3541 ttcccatctg gccgaagcca actatgccga ccttttttat gaccatcact ttctccttcc
     3601 atgttttcgg atttcgctca aaataagccg ataacgcgga tttgtcaaga tgcatgagga
     3661 gcgctggatc cgctcaaccg gattcatcca ccacgctaaa gcgggcgctg cgggtaagtg
     3721 ttttgcccag cacgaattcc tcgatcgtaa gggtgaattc atacaatccg ggctcaagtg
     3781 aggaaatatc gatcttcaat ggctcaaatc gatcggtgcg gatatcaccc cggtcggcag
     3841 tatgcgtgat gccggtcttt tgctttgagg ataatacgct cgcaagcatg ccgaagatat
     3901 ttccggggat atcctgctcc tgtatagtat aggtcatgcg gtaatgtgag cgtcccctga
     3961 tattcaaggg aaggtcgtag atttcgtaat agactatcag ggtatcgtcg gtcctgtatg
     4021 tattggtgaa attcggaatg agcttgatat cgcgccggtt aaagagcgag tctttttctt
     4081 tttcacccac ctttgaggcc aatatcaggc tggacatatg aaacccgctg tccctgaaat
     4141 cataaattcg taaaggtctc tcatagacat tcactctctg agccgatttt tgttcgatgg
     4201 cacaggcgat attcaggctg tcggccggag ctttcataga gatggtcgcg aaattgtaga
     4261 agtccggatt ttttgacagg gtgggggaaa tcacgatctg ctgaccggca gttttccgat
     4321 ccagaatggc gccctccaga tctataagtg ctgtcctgta ttctacggaa catacggcat
     4381 gtccctccac aggctcatag acaaattgat catagggtgt tgccacaaac aggtcgagca
     4441 tcgactcgcc ctcgggcccc ttgtaaatga agtatgaaaa gatcgagtta atcaggttgc
     4501 tgctctgggc gaatgtggcg acattgggaa tctcattcac cagatcatca gcgatcttca
     4561 catccccgcc gtaattcatg ggcgcgagct gataattatc tcccccgaaa atattcagga
     4621 actctaactc gaccggacgg ccgtcgaaca tatacaccca gctccagcgg cagctgagca
     4681 ggggatcgtt gtcatcggga agataccatt tcgcagataa gggctgacca tagcgaatat
     4741 agagtttgcc ctgatccatc tcccagccgt gcacgtccgc ttcgggacgg gcgaacataa
     4801 tttccgcgaa atagacacgc gcaaaatgct cgactatttt ttcgtttacc cgggtggtga
     4861 gatcggggtc gagaccggcc cagaaattgt cctcgaattg agctttttgg ctttccggta
     4921 actcctcata ttctctcttc tgctgatttg tcaaaagcag cgtgatatca cggtaaccgc
     4981 tgctctcatc ctcagacaga tcagagaggg cgcttgagaa aagagcgtag ctctggtcga
     5041 aatcccgcag cctatatttc aaataggcgt cgagcagcct tatttccgtc tgcaatgtgt
     5101 ccggccggtg tggtggggat atggttttaa cggcctggtt gtcggatttg aaaagcattt
     5161 cgatcagcgc ttttttatag gcgatgacat catcaccggg atagcgtccc tgcgagacca
     5221 gggcggtcct gtagccctgc gccagcttgg agcggttgcc gttgtggcag aagcgttcga
     5281 tacaaatatc catgatatga taataagcgc gcacataatt tgagtccctt tttacaagct
     5341 cctgaaactg ctcctcggcc gccgcctcca tatccgattc aagcagcagc aggccgagat
     5401 catagcgcca gtcgagcgaa tcctcctcca gccgaaccgc ctgcttgaga tatttgcggg
     5461 catagttgcg gttatgtaca ccccccatct tcgcgaagct catgcccagg atatgattta
     5521 ggtagggatc attatcatga tccctgagca tctcatacag aatattgcga gcctcttcat
     5581 aatcgccctt tttgtaatgc tcacatgccg tcagatacct gtcatgatcg tcagagcccg
     5641 gctccagcct gcgggcctgg agcgtgctta aaagtaacgc aagcgcgatc agcatgtaga
     5701 tcaaatgtct gtacataagc ctataatata taccacaaaa ccccgctttc gttgaataaa
     5761 aaggcaatat cttgggaaac cgcctctggg atttagatgg gggcgctctt tccgggtccc
     5821 attttatatt cccgaacagg cgttttttgt ccaaaaaaga ctaggcaaaa gctgttgagg
     5881 attttttatt tattgcaccg gtttggtgga aatattcatc tgagagaatt taggatagtg
     5941 gggtatttaa ccatctgcaa ggagttgtaa tttatgggta tgatgaagga gtttaaagaa
     6001 tttgcggtca agggcaatgt tgtggacatg gcagttggta tcatcatcgg tgtagctttt
     6061 gggactgttg taagctcgct ggttgccgat gtgatcatgc cgcctatcgg gcttctgctc
     6121 ggcaatgtcg acttcagcaa tttatttttt gttctgaagc agggggtcac agccggaccc
     6181 tatgctacag tgatggaagc gacggaggct ggtgcagtga cggtcaatta tggtgtcttt
     6241 ataaatacca taatcagttt tatcatagtg gcctttgcgg tatttatgct gatcaagaac
     6301 gtcaatcgta tggaacgcaa acaaccgccg ccgccccccc cctcaaccaa ggaatgcccg
     6361 atctgtttta ccaacattcc tattaaggcc acgcgctgtc cgaactgcac atctgaactg
     6421 cagacagcag aaggttaaaa ttctgattct ccggcaggag cccctgtttc tgccggatct
     6481 attggtccgt taatagctgt tttttaggca gggcaggacc cttcagtggt tctgccaata
     6541 gatatattgg ataggcagat tcaccgcgct tgtccagggc tatgcctttg atttcactcc
     6601 ggattctgcc cttgcttaca aaaccttctg cccgatcaaa ttctcaccac gcgcgagctt
     6661 ctctccgaaa tttttagcga tctggtaatg tgcatcgatc ggcccggatg atatcggaag
     6721 tgaggtcggc gcatccaccg cacccatcca gaccatgccc ataaagcgtg cggtggcctc
     6781 catggcaagt tgaaggtaat tggagctgtt catgatattc agtcccgaat gtgtggtgac
     6841 cagcgccaga cccttgccca tgaggtcaga tgaaaaatcg gtgtttattg tatccgccca
     6901 gcggtcgaca aaggtcttca tcctgccccc cagctgatac cagtaaaccg gactgaccag
     6961 gatgagaata tcggcggcaa acatcttatc cttgagtttc ggcatgtcgt catcgccggc
     7021 cttttcaaaa ttattctgaa gcgcgtcccc gaaataattg gaccagacaa aacttacctt
     7081 ataatcatga atatcgatct cttcgacttc agtattctcg gtcttgaggc cttcctggag
     7141 cgcgtccgct aaccttcggc tgttgccctt tccatccggg ctcccatata caatcaaaac
     7201 tttcatcagc ctcctcctcc tcagaatttt agatatctat ctatgacaga taacagcggg
     7261 gaggattcga tggttctttc aaaggatgat ttaaacctga caggggatta ttcggcggca
     7321 ggcttttaaa cctgccgcca aattcataga attgattgcg agtttatatt atgcagatta
     7381 tcctttcatg gaatcgagga aggccttgtt ggtcttggtc ttcttgattc gatccagcag
     7441 gaactccata ccttcgacgg gattcatctc cgccaggaac ttgcgcagaa tccagacctt
     7501 gttgagatca ttgctggaaa gcagaagctc ttccttacgg gtgccggaac ggtttatatc
     7561 catggccggg aagatacgtc ggtcggagag cctgcggtca agcacgatct ccatattacc
     7621 ggttcccttg aattcctcga agataacctc gtccatcctt gagccggtct cgatcagggc
     7681 ggtaccgata attgtgagcg agccgccctc ttcaatattt cgggcggcgc cgaagaatcg
     7741 tttcggcctg tgcagggcgt tggagtccac accgccggag aggattttgc cggagtgggg
     7801 cactaccgcg ttatgcgcac gagccagacg tgtaatcgag tcgagcagga tgactacatc
     7861 ctggccatgt tcgaccagcc tcttggcctt attgatgacc atgtcggcca cctgcacgtg
     7921 acgttccgcg ggctcatcaa aagttgacga gatcacctcg ccggaaacag aacgcgccat
     7981 ctccgtcacc tcttccgggc gctcatcgat cagaagcaca atcagcttga cttcgggctg
     8041 attgctgatg atggcgttgg cgattttctg caggatgatg gtcttaccgg ctttgggcgg
     8101 cgatacgatc agcccgcgct gtcccttgcc gatgggagtc atcaggtcca ttatccgggt
     8161 ggtccagtca tcattcttga attcgagttt cagcttgagg ttgggataaa ggggtgtgag
     8221 gttatcaaat aaaattttgt gtttggcgat ttcaggattt tcgaaattca ccgcttcgat
     8281 tttgagaagc gcaaaatagc gctccgactc cttgggcgga cgaacctgtc ccgatactgt
     8341 atcacccgtc ttcagatcga atcttttgat ctgtgagggc gagacgtaga tgtcatccgg
     8401 tccgggaaga taattgtaat cgggtgagcg caggaagccg taaccctcgt ccaggatttc
     8461 cagcactccc tcaccgaata tcagcccgtc tttttccgta tttgcttcca ggatcttaaa
     8521 tataagctcc tgttttcgga gtcccgatac cccggggata tccagctcct cagccgtctt
     8581 caaaagctcg gctatggttt tgtttttcag atcgacaatg tccattatcg caatcctttc
     8641 tatgaatgaa gaatataatt ttaaattttc tcaagataaa attgttactg ccttaaagga
     8701 ggattagtga tggtcgctcg gagaattact ggatgctttc cgtcggagca tcaccccata
     8761 atctttccag cccgtaaaac tcccgagctt cctgtagaaa cacatgcaca accacatcta
     8821 caaaatcgag aagaatccat cttccggtat cgaaaccttc gacattccat actctaatat
     8881 cttcttcttt cagtttttcg attattgttt cggcgattgc ccgaacctga acgtcggcat
     8941 cgccagagca gattacaaaa tagtcggtta tggatgtgat cttgcgcaaa tcaagaattt
     9001 gaacctcatt ggcttttcta agcagggcga attcgcctgc tttacgggcg aggtcgtatg
     9061 atttttctgc tgccaaatgc gctaattgat ctcctttcga ataggattaa ttgcttgccg
     9121 tcaagcgctt gccccagtca tggccgacta cgatggtgag atctgactga ggagcctcct
     9181 taccggacct gtcatctata atattatctt ttttgatatc gagctttcgg gccagccggg
     9241 ccgaaatacc ctcggctttg tctgtcttta ctattatcat gctttcattg acgttgaaat
     9301 tactgaaatt ctcggttttg acgatctcga atgtcatgct ctcgtccgaa agctgagtaa
     9361 gatgcatcgc aaactggttt gcagcccctt taattccgca gccgttcaaa atctctgtca
     9421 aaattaattc gggatggctt tcatattttt cgtaatctct tccaaccaca atcgtaatgt
     9481 ccagcgccaa aaagttgttt ttgagttcat tcatcgatat attgctttcc ggaatcccca
     9541 gctgctctgc aatcatatcc ttcttatcga gggccatggg atcccgcacc agcacaatag
     9601 tctgggacat cgcctcattc tcgaaattgg taatatcgat gacatcatat tttaagatat
     9661 cgctcgactg tttctcgacc cactcggcca ttttcccggc ggccccattg attccgctgc
     9721 cattcgctat ttgaactcgc aggacggtca gttccggcgt aatttcttta gtttcggccc
     9781 tcacaagcct gatggctgtg gaactcagga gcaccaccac aagaacagaa agcactataa
     9841 gcgtaatatc gaatagcctg ccctttgttt tgggtgcaat tctctgccgg tatttttttt
     9901 gtcgtggaat tttgcttttc tttttgcgaa gcatctaatc tacttctgaa gcaaccgtta
     9961 atagaaccag ttacggcgca tccggtcagt gttataataa tacatatatg ccggaacaga
    10021 atggtacccg atctcgagat agaatttatc ggacggcctg tattccaggc tgaagctggg
    10081 ataaatcgag ccataatctg tcggagtgcc cctgtcaccg cccagaagct gtccgggctg
    10141 atggacccat gccaggttga cattcaaggt caacgggttc gatatcctgt atctgatcga
    10201 gttcatgtac attccgatag tagttccctg tccgttggct gagaaatatg aaatcgtata
    10261 tgaatggctc atgtccagac gggacaggtc caaaagcgaa aatttattat ctattctcgc
    10321 ctttccgatt gcgtccgcgc tggtagcctc ttcctgacgt tccaggccgg attggcccaa
    10381 cacagggatc gacagaagca atatgaagag tgttgtgata accaaatgtc tcataaaatg
    10441 ctccttcacc accccctgtt atactgatat gaatcgaatt tgtttaagtt tgtcaagctt
    10501 taaattgcta taaattatac gccttagcaa gttcttctaa ttgttctctt gaatttatgc
    10561 ccagagcctc acggtaatca tcggttttga aggctgcggt ttttttgccc tgctttaaaa
    10621 agatctccat caggtctgtc aggtagtatt ctccctgggc gttactatta tcaatatgtt
    10681 tcaaattctc gaatagttcc ctcttccgga aaacaaatgt accggtgttg atctccccga
    10741 tccgcagttc ctcgccgctg caatccttat gttcgacaat cttttccaca aaatcctgtg
    10801 atttttttat gactctcccg taaccggtgg ggtcaggcgg ctcggaagtc aagacggtgg
    10861 cggcagcatc ctttctatta tgcaaatcca gcagattctt tatcgtttct ttgcggataa
    10921 agggcacatc tcccgccagc acgagaacat caccttcaaa atccgatagc gcttcctcag
    10981 ccatcattac agcatgtccg gttcccagct gtttctcctg caccgcaaaa tcatattcat
    11041 aatcagccag cgcctcttcg accaggtgcc gtttatatcc gaccacgacg accacctttg
    11101 caagccccat atctttcagg gtttcggcca gatattgaat tatcggctta ccctttaaat
    11161 catgcaatac cttgggaaga tctgatttca tcctgacccc cttgccggcg gccagtacta
    11221 tagctgctgt ctttttgatt tgatcaggca ctttttattc tgttgtcgtt atcgacagaa
    11281 atcacgcggg gtttatggcc ctgagcctca tcctcgctca agctgcaata agagattatt
    11341 atcagcttat cccctttttg acccagccgg gccgccgcgc cattgaggca aatcactccg
    11401 ctgtcggcct cgccctcgat tacatacgtc tccaaacgcg ccccggtatt gacattcaag
    11461 acctgcacct tctcatattc aagcaaaccg gtggcctcaa gcagggtctt gtcgattgta
    11521 atcgaaccct catactcgag gttggcctcg gttacagtgg ccctgtgtat tttggatttc
    11581 aaatattcaa cagtcatttt tcatcaccca gaaaaacgct aatattatca attagacgaa
    11641 cttcgtcaag ccataccgca agcgaaattg taaattttcc cgatagtctc ctgacctgtt
    11701 ccagcctctc atcatctgtt atagcgatat aatcgatctt tgttcccgcc atcctgtcga
    11761 tcattaatct catcgaccgc ttgattttag atgcattgcg ctcgccattt cggatcatat
    11821 ctcgggcatg catcaagctc ttgtatagaa cagttgcgcg cttcttctgt tcctcatcca
    11881 gatatttatt ccgggaggac aatgccaggc cggattcttc cctaactgtc ttgccgataa
    11941 cgattttgac cggcagattt aaatccctga ccatcttttt gatgattacc gactgctgat
    12001 aatccttctg cccgaaaacc gcaatatccg gctggacgat gttaaggagc ttggtgacaa
    12061 tggtcgcaac ccctctaaaa tggatcggcc tgaactgccc ctcgagaacc ttcgacatct
    12121 tttccagatt gacataggtc tcataatcag gcggatacgt cgattccctg tcgggataga
    12181 aaacataatc caccttcaaa tccctcaacc ggcgccggtc ttctttgaat gtccgcggat
    12241 atttcgaaaa atcttcagac ggcccgaact gggccggatt tacaaagatt gaaacgaccg
    12301 taacatcccc gagctttttg gctttccgga tcaaagccag atgaccctca tgaagcgcgc
    12361 ccatggtagg cacaaatgcg attttcttgc cctcgcattt gagccgtccg gccagtctct
    12421 gcatagattt taatgatttt acagtcttca tggcgattga gaaattgtgc ggttaacttt
    12481 tgagctaata gctttcatct ttggagggat agtcgccgga cctgacatct ttggcatatt
    12541 gctcgcccac ctcacggatt ttctgcccga gattgaaata gcgccttacg aatttggcat
    12601 agaaatcctc gtccatgccc atcaaatcgt ttatcaccat aatctggccg tcacaaccgg
    12661 ggccggcgcc aattccaatg gtgggaattt caattcgctt cgttatctcc tcggcaatct
    12721 tgctcttgac cgcttccagt acaatagaga aacatccagc ttcctgcagc gcttcagctg
    12781 attcgaagag gtaggtcttc tcggtttctt ctttgccctg cacacggtag ccgccgaatt
    12841 tgaagatcga ctgcggcgtc agtccgatat gccccatgac cggaatgccc gattccacaa
    12901 tccgcatgac cacatcgatc atttcaatcc cgccctcgag cttgaccgca tgcgctccgg
    12961 cctctttcag gaaccttccc gcattcctga cagcatcctc cagggagggc tgataggaca
    13021 tgaagggcat atctcccaca accatggcac gtttggcgcc acggcttaca gcctcggtat
    13081 ggtataagat ctgatccaac ggcagggaga gggtgttctc atggccatac acaaccatat
    13141 tgagcgaatc gcccaccagg aggccatccg ctccggcggc atccagctgc tttgcggtga
    13201 aataatcata ggcggtgaga aagacaattt tctcaccttc agactttttc ttgattaaac
    13261 ccgggactgt caccttatca gttttcacat atacctctaa tcgactttaa tgattaaagt
    13321 tccagcgaac cgggaggaac ataataatgc gttccggaga gcggtttctg gatctgctcg
    13381 accaggtcgt tgaaatcatt ggcattattg acaaaatcta tctcgtcggt tttgaccacc
    13441 agaagcgggg tcaggtcata ttcgaagaaa taatagttat aggcctcatt caactcgtcc
    13501 agataataga tatcgatatt tctttcaaac ggcacgcctc tctgcttgat tctctggtag
    13561 agagtctcgg tggcggcctg caggaaaatc accagatcag gtttgggtac atcccttgat
    13621 agcgaggcgg cgattttgtc ataaagaatc agctcccgct cctccaggtt gacctttgca
    13681 aaaagctgat ccttttcaaa ggtgtagtcg gagacaatga ggctgtgaaa aaggtcatga
    13741 gtgttgagtt tggccagctg ctggtaacgt gccagtaaaa agaatatctg ggtttgaaag
    13801 gcatacttct tcttatttct gtagaagtca gccagaaatg gattttctgt ggcttcctcc
    13861 ataatcaggt gtgcatccat tttctccgcc agcagcttcg ccaggctggt cttgcccact
    13921 cctattgggc cttccactgc aatgtaatta actgatttca tcttcattcc taaatcccag
    13981 gtctatttcc cgtttaatac tgacttgttt ttcccctttg atcctcttga gataggctct
    14041 gtagggcata ttggtggtgg gatcggctat atatggttcc aagtccagaa gcggcctcag
    14101 cgcaaactcg cgacgcgaaa ggccggggtg cgggattttc agtttctcgg actcgtggac
    14161 ctgtttcccg tagagcaaga tatcgatatc gatctcgcgc ggaccgtttt tgaattccgc
    14221 ttttttcccc acccgggatt caatctcctc caggtcagcc atcaattcct caggcggcaa
    14281 tttactgcta atctccacca ctatattcag aaagctgtcc tgatcggtga agcctaccgg
    14341 ctcggtttca tagaccggtg aaactctcag cacccctatt ttatcagagg ctttcatctt
    14401 ctccaatgcg gatttcagaa aattctcccg ctgtccgaga ttcgatccca ggccaaggta
    14461 aattctatct tcaggcataa attgaattta caaaagattg agcagttgtc aatcctaagt
    14521 tatggttttt ctatttcgat ctcgacatga tcgaggtttc cgggtatcgg ggggatcttt
    14581 ttgcggattc ttatagtcac tttttctggc gaaaactcct ccatgatttt atctgcaagc
    14641 agatatccca ccttttctat cagattatat ttcttgaagc taaaaagctc gttggtcata
    14701 tgataaattc tggtataact tatggtatcg gccaacttgt cgcttttggc cgctttagac
    14761 aaatcggcct gcacttcaac atcaacctca aaacgattgc ctgtctttct ctcggccgag
    14821 gaggtgccgt gataagcata gaataccata ttcgagactt taatcttgtc cgccatcggt
    14881 tcttctcttc gatttcatct tcttcggcac tttgtatatt ttggaataat tctcgatccg
    14941 tttgaaagcc tcctctattt tctcggaggg ttgtcccagc gccagacgta tgtaaccctc
    15001 gccttcctcg ccgaaaaacg atcccggcag cattataaca ccggtacgct tcaggagcat
    15061 gcggcagaag gtcagggcca cacggcgtcg atggggaagc tttatccaga caaaaggcgc
    15121 ata
//
LOCUS       DSEB01000256           14857 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000118, whole genome shotgun sequence.
ACCESSION   DSEB01000256 DSEB01000000
VERSION     DSEB01000256.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14857)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14857)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14857
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000118"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="ENO07_04695"
     CDS             complement(<1..1157)
                     /locus_tag="ENO07_04695"
                     /inference="COORDINATES: protein motif:HMM:PF02397.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01299.1"
                     /translation="MLKEKMLLLRRLHTVSDIILTILAYIAADVLISLLVHGNLPEFS
                     RVTISTQNLLVISGIWGFLALNQNSFYAYRAKNYGDMIKPVAIMVLKGISLFLTWIFA
                     AGSHLPGRLFIASFLALDFALLFSLRVLVVKFLRFSRARGKNCQQIIVVGKGVIARKI
                     IDDIKDNPEWGVRIRGILDVEDFDQLWRYRDIPQIGRLQQLPDIVQCNQVDYVVFAAN
                     RKYLDRFQEAVSLCEKMGVKACVLTDFFNLEYAEMQAGEFLFRPAIVYSRVREDRASN
                     FVKAVFDRVAASVVLLLASPVMALVALLIKITSGGPVFFSHERCGLNGRRFRLFKFRT
                     MVENAEQLKEKLRSKNEMDGPVFKISDDPRITRIGKFLRRTYMDELPQLFN"
     gene            complement(1219..3366)
                     /locus_tag="ENO07_04700"
     CDS             complement(1219..3366)
                     /locus_tag="ENO07_04700"
                     /inference="COORDINATES: protein motif:HMM:PF07940.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HDS01300.1"
                     /translation="MISPEAVFEPLSANEFERILCGRGGYSSARAFCDYASSREWILR
                     SSEIEDVCHLLRKNYPAFVKETIGGAEQVYENCFDVLGSGPTYLGAKIDWHLDFKSGR
                     IWKRDFYQDVPLMFWGDDSDARVPWELSRFQYLVGLALAYKLTNLNKYHHKFTALVED
                     WIYENPCPYGINWANAMEISIRVVNWLAAYELFDPKSFTDAFKLKFFHALYQHGHLIW
                     QNLAHCTPGLNNNHFLFDLAGLMVLGRLFYRTPDGRLWYEFARRELEKEVLNQISPDG
                     TCYESSLNYQVMMLEICLFVINFERSFGSDFPDEWKARIMQSCAALYTLSKNDHTVPN
                     FGDGGSDRLFKIVKRNERDITNILDLACVVIGLKGYCKNKISPVPELLLWTGKRGLRL
                     YFERLVSKQKEKRSMFFEDSGLAVIRDERSYLAFFANAAADLDFAGHKHNDLLAIEFS
                     YGNENFIVDSGTYVYTGDAACRNYFRKTASHSTLEVDGQEINRFLPKILFSVRKDAEI
                     NDVVWDSTPEFDSISVSHSGYMRLDGPIMTRRRIHFDKAESIYIMKDEFLGAGRHFFS
                     GNIILNHNITAGILDNQIVLKSASGPMAMIVMVSGKWHLEKIPHYISSSYGEKLESWK
                     IRYSLIDEAPQACIWGMFGADSYREMQQKSVQFYSILNRIGWRTKTVRKLELKRGSEQ
                     LLPERVIDQILNPGLEQLRESRVVMEEADKSSE"
     gene            complement(3462..4637)
                     /locus_tag="ENO07_04705"
     CDS             complement(3462..4637)
                     /locus_tag="ENO07_04705"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDS01301.1"
                     /translation="MIKILLVAGARPDFMKLAPLYFELRKYPSVFSLRIVHTGQHYDY
                     AMSRVFFDQFGLPEPHFFLEAGSGSHAQQTGNIMIRVEDIMRSEKPNMAVVFGDVNST
                     LAAVLAASKLCIPIAHVEAGLRSHDRSMPEEINRVVCDTLADMLFTTCDDANLNLIKE
                     GIDVGRIFLVGNIMIDTLKYFLPQAEKSRVLDKLGIDEGKYILVTLHRPSNVDNLENL
                     DRLAEILSAAAERCKIIFPIHPRTRKNLENNGGNSAILNNRNIILTDPLGYFDFIKLQ
                     KNAFIVMTDSGGIQEETTYLGVPCLTLRKNTERSVTVTDGTNKVVALNLKKIISLIDY
                     YMQDGVLSRRRPFLWDGQTAERIVRILRERFADEAKKEDEAERLTSTDKIVLKNITE"
     gene            complement(4634..5638)
                     /locus_tag="ENO07_04710"
     CDS             complement(4634..5638)
                     /locus_tag="ENO07_04710"
                     /inference="COORDINATES: protein motif:HMM:PF02706.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01302.1"
                     /translation="MAARKIKNKVRSQEGSSEVYLSVLSVLSDKKLIVLSILVAAFFC
                     AFIFGIIYKRNYTASVYIYSKPGNEIASDLNRNAQDDHSIFLENGEIPIETYSAILRS
                     PRIQMEILQAKYSVNFKYKHYDMDLLQYFSLNNVSYALQELNDITEINALDNSGVLKI
                     SVTTQYPDLSQQIAESYVNALEEYLEKRRQQKIEEKISLLDKADEAAPEHEQAEPGEG
                     GGLTEKLEGQETPLMLARSSGKFRPGSIRSARITSNNGLLKSAVTAMSLAALLILIPS
                     IVALNWRKSTGAIKMTGNGMRRVILGRGAELSPEKAAPQTAKKTATREGTQNKRIVKV
                     "
     gene            complement(5648..8044)
                     /locus_tag="ENO07_04715"
     CDS             complement(5648..8044)
                     /locus_tag="ENO07_04715"
                     /inference="COORDINATES: protein
                     motif:HMM:PF02563.14,HMM:PF06251.9,HMM:PF10531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01303.1"
                     /translation="MNKDAVLAYTLLFFFCVTVISLPAQSGSDTEGVLITPQSMGAGQ
                     EKTQSERYSTPPIYENIPSTGSIRVEQSNGALNIPRETERGDTGTDGNADESVSKTDP
                     GEQVIPKPGPDGLELFGANLFSSASADFSPAPIATVPPDYKLGPGDNLIVYLWGRIDL
                     EMDLTVDREGKVFVPKAGEIVAFNLTLEQFKQRLSKKLSTIYSDFELGVMLGRIRTIR
                     VYVYGEVKKPGAYTVSSLSTLFNALHYSGGPNENGSLRKIRLSRGKETNATVDLYDFL
                     LHGKNTAGIKLQSEDVIFVPVVGPKVSVRGEVKRPAIYELKNQEKLLDVIELAGGAAD
                     EAYLGRVMIDRINNGQGRKLIDLSLATAEDKKTNNIEIKAGDDISVFSIYERRENVVW
                     LGGHVKHPGAYEMDNGLTISELLEDGRQLKENPYMERADLIRTSPDSQKELIAINLAW
                     ALNKSAEHDLKLRINDSLRIYGSNEIARKKYVNIDGEIARPGEYQLFKNMRISDLIFM
                     AGGLQRGAYTLTAEVARRLPGKEIELIYLDLEKMKNGGSGEDILLAEDDYVFIRRIPS
                     WELNNFVSITGEVEFPGKYALYRKDETLYDLIRRAGGLTPEAYAFGTVFIRGEIEDKL
                     KRMNLPGILDRSNPVRFDSAGNYSQTSILKYNPGELSRLIIDVDLILASKGEEGDVTL
                     QMGDEVYIPPRPSGVQVTGAVASSGTIHFAPEKKAQYYIDRAGGLLPHADKDNIRVIR
                     ANGQVVPNGKARRYKLGLGDVVLVPSKIIKEKSLGKTILTSVSIISSIATTIFILDRL
                     "
     gene            complement(8232..9134)
                     /locus_tag="ENO07_04720"
     CDS             complement(8232..9134)
                     /locus_tag="ENO07_04720"
                     /inference="COORDINATES: protein motif:HMM:PF04321.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDS01304.1"
                     /translation="MANSPQKLLLTGAAGFLGQALLKKFHAHYDMAAVINRTEIDDPP
                     DGVEIVRTDLSEKKAVNELVRTQEPDIIVNTAAWVDVDGCESDRKRALKSNFSIVENL
                     INAIKDSRIYFIQLSTDYIFNGVDHPANVDDGPAPLNYYGRTKLMAEEYITDNYARYL
                     IARTCALFGMPNRGQTNLINYFYDSLKAGKEVQAPEDMYANPIWVENLAELLLEAIQK
                     GYKGMLHLGGKDYLSRYEFARIFARIFGFDKDLVKPIAAIYHKRPARRPRYAGLNVEQ
                     THEKLDIPLLSADEALSAIHDRMK"
     gene            9264..9986
                     /locus_tag="ENO07_04725"
     CDS             9264..9986
                     /locus_tag="ENO07_04725"
                     /inference="COORDINATES: protein motif:HMM:PF03009.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDS01305.1"
                     /translation="MIPLICAHRGASHKYAENTLEAFRKAVEQRADSIEVDVRQTRDG
                     ELVACHDFNLSRLTGIAADLSEISLDDFKKLRIMGQEPPATLEEIVSEIGNPVSIVFD
                     IKENHLEQKILKLIRDKSLEDRAIVSSFDPRILANIKLLNPEIRTALIAGPISIMPLA
                     VNVCFYLRRVSEYIKSDYMHLNYMNILYRGYRTLSRWGYKISYWTVDHPRDIRNAVSL
                     KPELIITNRPGLARAVLEKAMR"
     gene            complement(10025..10498)
                     /locus_tag="ENO07_04730"
     CDS             complement(10025..10498)
                     /locus_tag="ENO07_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligand-binding protein SH3"
                     /protein_id="HDS01306.1"
                     /translation="MSPYIQAFIISLLPVAELRGGIPLAVANGISLVTAFFLCVIANM
                     LVIPIFYTFLKTLHNLFYKVRVYQLVFDRYVHRTLNRAEQKVRRYGYWGVMLFVAIPL
                     PVTGAYTGTLAAWLLKLDMKKAFWFLALGVVIAGIIVSIAVMTGVGILRIFVKPS"
     gene            complement(10652..11830)
                     /locus_tag="ENO07_04735"
     CDS             complement(10652..11830)
                     /locus_tag="ENO07_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01307.1"
                     /translation="MKRALIILAFALFALGTTAGNGASIDFDLYGSLKLDGAYDQNLT
                     SHGNFVMWVDPQQFEDNDEQYNMTANESRFGIMLTGNEYENAEVNGKIEFDLYAGSSS
                     GAVAENEPMLLLRHAYFSVQMGGTKIIAGQTYDMISPLNPSTLNYPVLWGCGNIGYRR
                     PQISLWQSFGSGENTDMTIAGGFFRTIGSDLTPTFTLAAGESADGTDDGTDAGIPSFQ
                     GMFDVKHNFSSGSSARFGISGLWGTLKSETNLGGSENYDSWAINGHMSFAFPNGYGFM
                     GEIYTGSNLQSYLGGILNNSSIEGVNSTGGWASAWMQASNRVKLSAGFGMDTVDKEDI
                     GTGRSKNQSIFGNVNYTLVENVILGAELSYWDTEYVNIDESTDSYDNLRLQTSMILNF
                     "
BASE COUNT         3781 a         3811 c         3409 g         3856 t
ORIGIN      
        1 atattaaaaa gctgaggcaa ttcatccatg taggtcctcc tcagaaattt gcctattctg
       61 gtaatgcggg gatcatcact gatcttaaac accgggccat ccatctcgtt tttggatcgc
      121 agtttctctt taagctgttc ggcattttca accatcgtgc ggaacttgaa taaccggaaa
      181 cggcgtccat taaggccgca tctctcgtgg gagaaaaaca ccggaccgcc ggatgtgatc
      241 tttatcagaa gggccacgag agccataacg ggcgaagcca gaagcaggac aacagatgcc
      301 gcgacccggt cgaaaacggc tttaacaaaa ttcgaggcgc ggtcctccct tacccttgaa
      361 tagacaattg caggacggaa taaaaactct cccgcttgca tttcagcata ttccaagttg
      421 aagaaatcgg tcaggacaca ggccttgaca cccatcttct cacacaggga caccgcctcc
      481 tgaaatctat ctaaatattt tctgttggcc gcgaatacaa cataatcaac ctgattgcac
      541 tggactatgt caggcagttg ctgcaggcgg ccgatctgcg ggatatcacg atatctccag
      601 agctgatcaa aatcctcgac atcaaggatg cccctgatcc tgacgcccca ttcaggatta
      661 tccttgatgt catcgataat ctttctggcg atgacgcctt tgccgaccac aataatctgc
      721 tggcagtttt tgccgcgagc gcggctgaac cgcagaaatt tgaccaccag taccctgagc
      781 gaaaatagca gtgcgaaatc aagggcgaga aaggatgcta tgaatagtcg tcccggaaga
      841 tgactcccgg cggcaaaaat ccaggtcaaa aacagtgata tacccttcaa aaccatgatt
      901 gcaaccggct tgatcatatc accatagttt tttgcacgat aggcgtagaa tgaattctga
      961 ttcagggcca ggaaacccca gatgccggag atgaccagga gattttgagt ggaaattgta
     1021 acccgggaaa attcggggag attgccgtga accagcagag atatcagcac atccgctgcg
     1081 atgtaagcga ggattgtcag gattatatcg ctgaccgtgt gcagacgcct gagcaatagc
     1141 atcttctctt tcaacatgac tgaacttctc tttgttgatt tccctcttac attctggcaa
     1201 taatttcgat atgtttgcct attccgaact cttatccgct tcttccatga caacgcggct
     1261 ttcccgcaat tgctctaatc ccggattgag aatttgatct ataacccgct ccggtaatag
     1321 ctgctcagaa ccgcgtttga gctccagctt gcggaccgtc ttcgtacgcc atccgatacg
     1381 attgagaata gaataaaact gaaccgactt ttgctgcatc tctctgtacg aatcggctcc
     1441 aaacatgccc cagatacagg cctgcggggc ctcgtcgatc aaggaataac ggattttcca
     1501 gctctccagt ttctcaccgt agctgctgga aatatagtgg gggatctttt ccagatgcca
     1561 ttttcccgaa accatcacga tcatcgccat agggccggag gcagatttga gcacgatttg
     1621 gttatccaga attcctgcgg ttatgttgtg atttagaata atgttcccgc tgaaaaagtg
     1681 ccggcccgct ccaagaaatt catctttcat aatatagatt gactcagcct tatcgaaatg
     1741 tatcctgcgc ctggtcatta tcgggccgtc gaggcgcata tatcccgaat gcgaaactga
     1801 gatagaatca aattcgggag ttgaatccca gacaacatca ttaatttccg catcttttct
     1861 taccgagaac aggatcttcg gcaaaaatcg gtttatctcc tggccatcaa cttccagcgt
     1921 ggaatgcgag gctgtctttc ggaaatagtt tctgcaggcc gcatcgccgg tatatacgta
     1981 agtcccggaa tcgacaatga aattttcatt tccataggaa aactctattg ccagcaggtc
     2041 attgtgtttg tgaccggcga aatcaaggtc cgcggcggca tttgcaaaaa aagccagata
     2101 gcttcgctca tcacgtataa cggctaagcc ggaatcctca aaaaacatgg aacgcttctc
     2161 tttctgcttt gaaacaagcc gctcgaaata cagcctgaga cctcttttcc ctgtccataa
     2221 caggagttca ggtactggac ttatcttatt cttgcaatat cccttgagtc cgattaccac
     2281 acatgcgaga tcaagaatat tggtgatgtc acgctcatta cgcttgacaa tcttaaataa
     2341 tctatcagaa ccgccgtctc caaaattcgg aacggtatgg tcatttttgg atagagtata
     2401 gagcgcagca cagctctgca taatccgcgc tttccattca tccgggaaat ctgacccaaa
     2461 acttctctca aaattgatta caaagagaca tatttccaac atcatgacct gataattcag
     2521 agatgactca tagcaggtac catcgggact tatctgatta agtacctctt tttcgagttc
     2581 tcttctcgcg aactcatacc agaggcggcc gtccggagtt cggtagaaca gcctgcctaa
     2641 gaccataaga cccgccagat cgaacaagaa gtgattgttg ttcaagccgg gggtgcagtg
     2701 cgccaggttc tgccagatca aatggccatg ttgataaaga gcatggaaga atttcaattt
     2761 gaaagcatcc gtaaaagatt ttggatcgaa aagctcataa gctgccagcc agttcacgac
     2821 gcggattgaa atctccatgg cattggccca gttgattccg taagggcagg gattctcata
     2881 aatccaatcc tcgaccagcg cagtaaactt gtgatgatat ttgttcagat tggtgagttt
     2941 gtatgccagc gccaggccca ccaggtattg aaagcgggag agctcccacg gtaccctggc
     3001 atcagagtcg tcgccccaga acatcaaggg cacatcctga tagaaatctc tcttccagat
     3061 tctccccgac ttaaaatcaa gatgccagtc aatttttgcc cccaggtagg tgggaccgct
     3121 gcccagaaca tcgaagcagt tttcatagac ttgctccgcg cctccaatcg tctctttaac
     3181 aaaagcggga taattcttgc gaagcaaatg acagacatcc tctatttcag aggagcggag
     3241 aatccactct cttgaagagg catagtcaca aaaagcccga gccgaggaat agccgcctct
     3301 tccgcacaat atgcgctcaa attcattggc gcttaagggt tcgaaaactg cttcagggct
     3361 aatcataaat ccccaaaaat cccccgatgg aaaaagcctc ttgccggtca ctttcaggtg
     3421 aatgcaatac gctttcccat tatgaaattc aagatcaaaa tctattcagt aatattcttc
     3481 aatactattt tatcggtcga ggtcagtctc tcggcttcat cctccttttt ggcctcatct
     3541 gcgaaacgtt cacgcaggat tcgcactatc ctctcggccg tctgaccatc ccacaggaaa
     3601 gggcgtctcc gggacagcac accgtcctgc atataataat ctatcaggct tattattttc
     3661 ttcaggttca atgccactac tttattcgtt ccatcggtta ctgtaactga acgctcggtg
     3721 tttttgcgca gcgtcaaaca gggtacgccc agataagtgg tttcttcctg gatgcctccc
     3781 gaatccgtca ttacaataaa tgcatttttc tgcagcttga tgaaatcgaa ataccccagc
     3841 ggatctgtga gaataatatt cctattattc agaatggcgc tgttccctcc gttattttcg
     3901 agattttttc ttgtcctggg atgaatcgga aatattattt tgcagcgctc ggccgcggcg
     3961 ctgaggatct cagcgagcct gtcaagattc tcgagattat ctacgttcga gggccgatgc
     4021 agggtgacga gtatatattt accctcatca atccccagtt tgtcgagaac cctggatttt
     4081 tccgcctggg gtaagaagta tttcagggta tcgatcatga tatttccgac caggaatatc
     4141 ctgcccacat cgataccctc tttgatcagg ttcaaattag catcatcgca ggttgtaaac
     4201 agcatatccg cgagcgtatc gcagacaacc ctgtttatct cttcgggcat actcctgtca
     4261 tgggagcgca gaccggcttc cacatgggca atcgggatac acagctttga ggccgccagc
     4321 accgcagcca gggtggaatt gacatcacca aaaacaaccg ccatattcgg cttttcggat
     4381 cgcataatat cttcgactct gatcatgata ttaccggttt gctgcgcatg gctgcccgat
     4441 cccgcctcca ggaagaaatg cggctcgggc aggccaaact gatcaaaaaa cacacgcgac
     4501 atggcgtagt cataatgctg cccggtatgg acgatgcgca aactaaaaac ggatggatat
     4561 ttgcgaagct caaaatacaa gggcgccagc ttcataaaat caggccttgc acctgctaca
     4621 agtaatattt ttatcatact ttgactatcc ttttgttttg agtaccctct cgggtggctg
     4681 tctttttcgc ggtttggggc gcagcttttt cgggactcaa ttccgcgccc cgtccgagaa
     4741 ttaccctgcg cattccgtta cccgtcatct ttattgcccc tgtacttttt ctccaattca
     4801 aagcgactat ggatgggatc agtatcaata aggctgccag agacatagca gtaacggcgg
     4861 attttaacag accattattg gacgtgatac gggccgacct gatactcccc ggcctgaatt
     4921 taccgctgct cctggccagc atcagcggtg tctcctgacc ttccaacttt tctgttaagc
     4981 cgcccccctc ccctggttct gcctgctcat gctccggggc tgcctcatca gccttatcca
     5041 gcaggctgat cttctcttcg atcttctgct gccgtctttt ctccagatat tcctccagtg
     5101 cattcacata actctctgca atctgctgcg ataaatcggg gtattgcgtt gttacagata
     5161 ttttcagcac accggaatta tcgagcgcgt ttatttccgt aatatcattt aattcctgca
     5221 aggcatagct gacgttattg agggaaaagt actgcaacag gtccatgtca tagtgtttat
     5281 acttgaagtt caccgagtat tttgcctgca gtatttccat ctggatccgg ggagatctta
     5341 gtattgcaga atatgtttca atcggtattt cgccattttc gagaaagata ctgtgatcat
     5401 cctgggcatt gcggttcagg tcactggcga tttcattgcc gggcttggag tagatataaa
     5461 ccgaggctgt ataattgcgc ttgtagatga tgccgaaaat aaaagcacag aaaaaggcgg
     5521 ccaccagaat cgacaacacg atcagtttct tatctgaaag aacgcttaaa accgagagat
     5581 atacttcact gctgccttct tggcttctta ctttattttt aatttttctc gctgccatga
     5641 cctccgccta cagcctgtcc aaaataaata tcgtggttgc tatgctggat atgatcgaaa
     5701 ctgatgtcag gatcgtctta cccaggctct tttccttaat aatcttggat ggcaccagga
     5761 ccacatctcc gagccccagc ttatagcgtc tggctttgcc attaggcaca acctgaccat
     5821 tggccctgat taccctgata ttatccttat cggcatgcgg cagcaaacct cccgccctgt
     5881 cgatgtaata ttgggccttc ttctcggggg cgaagtggat cgttcccgac gatgccacag
     5941 cgcctgttac ctgcactccc gaggggcgcg ggggtatata gacttcatcc cccatttgca
     6001 aagtcacgtc tccttcttct cctttgctgg caagaatcaa gtcaacatcg attatcaaac
     6061 gcgaaagttc tccgggattg tacttgagaa tcgaagtctg gctatagttc ccggccgaat
     6121 caaatctgac cggattggag cggtccaata tgccgggcag gttcatcctt ttcagtttat
     6181 cttctatttc tccccttata aacactgttc cgaaagcgta ggcttccggg gtcaaaccgc
     6241 ccgctctccg gataagatcg tagagcgttt catccttgcg atagagagca tatttgcctg
     6301 gaaattcaac ctcacctgta atgctcacaa aattattcaa ttcccaggag ggaattctgc
     6361 ggatgaaaac ataatcatct tccgccagga gaatatcctc acccgaaccg ccattcttca
     6421 tcttttccag gtcgagataa atcagctcga tctcttttcc gggcagacgc cgcgcgacct
     6481 cggccgtgag ggtgtaggct ccgcgctgga gacccccggc cataaaaatc aaatcggata
     6541 tacgcatatt cttgaagagc tggtattccc cgggcctggc gatttctcca tcgatgttca
     6601 catatttctt cctagcaatt tcattacttc catatatcct caggctgtca ttgattctga
     6661 gcttgagatc atgttccgca ctcttattga gagcccatgc caggttgatg gcgatcagct
     6721 ccttctggct gtcagggctg gtcctgatca ggtcggccct ttccatgtag ggattctcct
     6781 tgagctgcct gccatcctcc agaagctcgg aaatggtcaa tccgttatcc atttcatagg
     6841 cgcccggatg tttcacatgt cccccgagcc agacaacatt ttcgcgcctt tcatatatgc
     6901 tgaagactga gatatcatcc ccggccttaa tttcgatatt gttggttttt ttatcctcgg
     6961 cagtggcgag ggataaatct atcaatttgc gtccctggcc gttattgatg cgatcgatca
     7021 tcacccggcc gaggtaagcc tcgtcggccg caccaccggc cagttcaatc acgtccagaa
     7081 gcttttcctg attcttcagt tcatagattg cgggacgttt tacctctccc cggaccgaga
     7141 ctttaggccc gacaacaggg acaaagatga catcctcgga ctggagtttg attcccgccg
     7201 tgttttttcc gtgaagcagg aaatcgtata aatccacggt tgcgttcgtc tctttgcctc
     7261 ggctcagacg gatttttcgc agggagccat tttcattggg gccgcccgaa taatgcaggg
     7321 cgttgaataa ggtggaaagc gagctaacag tatatgctcc gggtttcttg acctcgccat
     7381 agacgtacac cctgattgtg cggattcgtc ccagcatgac ccccagctcg aaatcagaat
     7441 agatggttga caattttttg cttaaacgct gcttgaactg ctccagggtc aggttgaagg
     7501 ccacaatttc gcccgccttg ggcacaaaga ctttcccctc acggtcgaca gtcaggtcca
     7561 tctcaagatc gattcgcccc cacagataga caatcagatt atcgcccgga cccaatttat
     7621 agtcaggcgg gactgtggcg atcggggcgg gcgaaaaatc cgcagaggcg gaactgaaca
     7681 gattggcacc gaagagttcc aggccgtccg gaccgggttt ggggatgacc tgctccccgg
     7741 gatcagtttt ggaaactgac tcatccgcat ttccatcagt tccggtatcc cctctttctg
     7801 tctctctcgg gatattcaat gccccgtttg attgctccac ccggattgaa cccgtggatg
     7861 gaatgttttc atatatgggc ggtgtggaat aacgctcgga ttgtgtcttc tcctgcccgg
     7921 cacccatgga ttggggggta atcagaacac cttcagtatc cgagccgctt tgggcaggaa
     7981 gggatattac tgtgacgcag aagaaaaaca aaagagtgta agctaaaacc gcatctttat
     8041 tcattcatgc ctctctcaaa tcctatttta aggcagattc tctgggacta gtctatgcaa
     8101 gctcagtgcc gaacttggcg gcaatagcta tccgtgtgcg gcagaatgaa ttacaagcaa
     8161 ggcaacgtgg gatgaaaatt tctattgttg aaattgagag aaaaaattaa cgatcggcat
     8221 ggaaattttt tctacttcat gcggtcatga attgcagaca gagcttcatc cgcgctcaac
     8281 agagggatat ccagcttctc atgagtctgt tcgacattca agccggcata tcgcggcctg
     8341 cgggcgggac gtttatgata aatagcggct attggtttga ccaggtcctt atcaaatccg
     8401 aaaatccggg caaaaatacg cgcaaactca taacgggaaa ggtagtcttt accccccaga
     8461 tggagcatgc ccttatagcc tttctgtatc gcttcgagca gaagctcggc cagattttcg
     8521 acccagatcg gattggcata catatcctcc ggggcctgaa cctccttgcc cgctttcagg
     8581 ctgtcgtaga aatagtttat caggttggtc tggccccgat tgggcatacc gaaaagcgcg
     8641 caggttctgg caatcaggta gcgggcataa ttatcggtga tgtattcctc tgccatcaat
     8701 ttggttcggc cgtaataatt caatggggcc ggcccgtcat ccacattagc aggatgatcg
     8761 acaccgttaa aaatgtaatc ggttgacagc tgtatgaaat agattcttga gtccttgata
     8821 gcgttgatta gattttcgac gattgaaaaa tttgacttga gagcgcgttt gcggtccgat
     8881 tcgcatccat ctacatcaac ccaagccgca gtattgacaa taatatcagg ttcctgcgta
     8941 cgaacaagtt cattgacagc tttcttctcg gacaggtcag tccgtacgat ttcaacgcca
     9001 tccggcggat catctatctc ggtcctgttg attaccgccg ccatatcata atgagcgtgg
     9061 aatttcttga gcagagcctg ccccagaaat ccggcggccc cggtcaaaag caatttttga
     9121 ggagaattag ccatatcatc attctcacta acgatgcaaa aagattttat aatctactca
     9181 ttctcagttt tcggtcaagt aaactttttt gctttataca gattaaactt gccaaataaa
     9241 aatattttgc tcaacttctg gaaatgattc ccttgatctg cgcccataga ggcgcctcgc
     9301 acaaatatgc agagaataca ctcgaggcct tcagaaaagc ggtcgaacag agggctgatt
     9361 cgatcgaggt cgatgtgcgc cagacccggg atggagagct ggtggcctgc catgatttca
     9421 atctctcgcg ccttaccggg attgccgctg atctctcgga gataagcctg gatgatttca
     9481 aaaagctccg gatcatgggc caggagcctc ccgcaacgct ggaggaaatc gtatcggaaa
     9541 tcggaaatcc tgtttccatc gtcttcgata taaaggaaaa tcatctggag caaaaaatcc
     9601 tgaagttgat tcgggataaa tcgttggaag acagggcgat tgtctccagc ttcgatcccc
     9661 gcatcctggc caatataaaa ctcctgaatc cggagataag gaccgccctg attgccggac
     9721 ctatttccat tatgcccctt gcggtgaatg tctgcttcta cctgcgcagg gtatcagaat
     9781 atataaaatc agattatatg catttgaact atatgaatat tttgtaccgc ggttaccgca
     9841 ccctttcacg ctggggctac aaaatatctt actggacagt ggaccatccg cgcgatatca
     9901 ggaatgccgt aagtctcaag cccgagctca taatcaccaa ccgtccgggg ctcgccagag
     9961 cggtactgga aaaggccatg cgataaaggc cgctctgtta ccctgtgctt ctgaataatg
    10021 acttttatga gggcttcacg aagattctca gaatgcccac accggtcata accgctatac
    10081 ttactattat tccggcaatt acaactccca gggcgagaaa ccagaaggcc tttttcatgt
    10141 cgagtttaag cagccaggcg gccagagtgc cggtataagc tccggtcacc ggcaggggta
    10201 tggccacaaa gagcatcacg ccccagtaac catatctcct gaccttctgc tcggcccggt
    10261 tcagggtcct gtgaacatat ctgtcaaaaa caagctgata aactcggact ttataaaaaa
    10321 ggttatgcag ggttttcaga aaagtataga agatggggat cacgagcatg ttcgcgatga
    10381 cacacagaaa aaaagcggtg accagggaga ttccattcgc aacagccagg ggaattccgc
    10441 ccctcagttc cgctacagga aggagagaga ttatgaaagc ctgaatataa ggactcatcc
    10501 aatattatac ttttttgcgt tcatttgaat acagtatttt gatttcagga ttataaccct
    10561 gttaaaaaaa agatgcaaac catatctgaa agattcagat acggtttgca tccaaaatta
    10621 acctgctctt attcctgttt taatctcagg atcagaaatt gagaatcatc gaggtctgca
    10681 gacgcagatt atcatagctg tctgtagatt catcgatatt cacatattcg gtatcccagt
    10741 acgacagctc ggcgcccagg ataacattct cgaccagcgt gtaattgaca ttgccgaaaa
    10801 tgctctggtt cttggaacgt ccggtgccga tatcctcttt gtccacagta tccatgccga
    10861 aaccggcaga gagtttgact ctgttggaag cctgcatcca ggcggaggcc cagccgccgg
    10921 tggagttgac gccctcgatg ctgctgttat tgagtatgcc tcccaggtag ctctgcaaat
    10981 ttgaaccggt atagatttcg cccatgaaac catagccatt cgggaatgcg aaagacatat
    11041 ggccatttat ggcccagcta tcataattct ccgatccgcc taagttggtc tcggacttga
    11101 gagtacccca gagtcccgag ataccaaagc gggccgatga acccgagctg aaattgtgtt
    11161 tcacatcaaa cattccctgg aatgacggaa ttccggcgtc agtaccgtca tcagttccgt
    11221 ccgcactctc gcctgcggcc agggtgaatg tcggagtgag gtcgctgccg atagtgcgga
    11281 aaaatccgcc ggcaatggtc atgtcagtat tttcaccgct tccaaatgac tgccagagac
    11341 tgatttgcgg cctgcgataa ccgatatttc cgcaacccca gagaaccggg tagttcaatg
    11401 ttgacggatt gagcggcgag atcatgtcat aggtctggcc agcaatgatc ttggtcccgc
    11461 ccatctggac cgaaaaataa gcatgcctga gcagaagcat aggctcattc tcggctaccg
    11521 ctcctgagga ggagcccgca taaagatcga attcgatctt accgttgact tcggcattct
    11581 catactcatt cccggtaagc ataatgccga atctggattc gttggcagtc atgttatact
    11641 gctcgtcatt atcctcgaat tgctgaggat cgacccacat gacaaagtta ccatgactgg
    11701 tcagattttg atcataggct ccatcgagct tgagggaacc atagagatca aaatcaatag
    11761 aggctccgtt ccctgcggtt gtgccgagag caaaaagagc aaaagctaaa atgataagtg
    11821 ctcttttcat catccctcct tagattaacg attttttagt tgaattctca gattatccta
    11881 ctcaccctac tattgataga atagcggacg tgctattccc tttggatgtc aacctttatg
    11941 attaatttcg gctaaaatcg ccaatactta agaaaaaaga gactaaaaaa aggccccgtc
    12001 aggttggaaa taacggggcc aggatagact tataagttat tcaatacaat gggattaagg
    12061 acattccgca cacggctcgg ctccgcctgc gaagacataa ttgattatgt aaaccgcatc
    12121 tgagacattg acgtcggcat cacagttcac atccgcagcc tgtaacggct cgggcgctgt
    12181 tcctccaatg aaaacaaaat tgataatgta aacggcatcg ctcacattta cagtctcgct
    12241 gccgtcagca tcaccacaaa tatactgcgg ttcagccata acctccagtt cagccggaat
    12301 gaccacggca gcgttatccg ggtcattgtt ggcaatgata atatcggaga tgtaggttcc
    12361 gggttccagg gactcggttg ttacaaaatc gcaatggatt gtatctgccg cacctcccgg
    12421 tatttcgcct tcggtcttgt cgagaagcag ccagtcatag ggcttgaaga attcgattgc
    12481 aaggttctct tgcaggtaag gggcatcata aaccacctcg agaccgtcat tacccagagc
    12541 attttcaata cccacgctgg cattgttgac atcaaatccg ggagctattg atctatattg
    12601 atatttaatg cgaccatcac tgcttataat gacttccatt gtgatcacat cgcccggatc
    12661 tgccctgtat tcaggataat cctcaaactg gatcacacag cgctcgccgt tggtatgcag
    12721 gaatacatgc gcattcgtat tgtcatagtc agttgggtcg agatcatccc acataaatgc
    12781 gagaatatta tttggtgccg tagcggtcgg aatcggcctt cctatacggg cttccatact
    12841 ggtttcgtca aaaccgatta ttccgttgga gccgatataa atctggctgt aggtgttgcc
    12901 gtagtaagtg aactcgaatc cgaggtcaaa cggtccgata tagttgtcat catccagctg
    12961 gccaaccagg tcggttccca tcgaggagac atcgatccac tcgtaaaccg ggcctccgga
    13021 ttcatccgaa tccagccagt agtatccgaa atcatcggga ccgcccttac tctcctccac
    13081 cgagctgccg agataatcaa tatcgcttcc cttgggctcg tcatcggtgt aaacataatc
    13141 gggataatcg cggctggccg gggcaaattc tccggtgccg gaccccttcg caaaaatacc
    13201 atcttttaca acatgcgggc tgattctgat attgtataac aatccccccg gaccggtatt
    13261 ttcaataatc aactcagcct ggctggagtc tccctgcatc aggctttcgc tgaaagcccc
    13321 gggtgaagca tttatttccg gagcccgatc gagacggatg ttgtcgataa accagtcatc
    13381 ctctcccgca tcggatccga atgtgcggaa cctgatctgg agattgctgt gatgagcatc
    13441 cacaggcagg gcaatattta catattcaaa atcagtcatc gcttcgcccc cgccttcatg
    13501 aaccataagg ttggtccaga ctcccgccga attgcggtaa tcgatccaga gattatcgcc
    13561 gggctcgggc acatcctcat caccgcccat ctcataataa tatgaaagaa gcacatcatt
    13621 gaaggctgaa atatcgatta actgggttgc catagtatcg ctgccgccat cgagattgta
    13681 gctgtacggc agggacggcg tccccggagc ggaatagcgt atcaagcccc cgatataagt
    13741 gaaccagcgc tgggtataga gtgaatcatc ggggaatgtt tcgtaccagg gaatttccgc
    13801 cggtgtgccg gctgaaactg tctcgatcat ggcataagcg gtaatattat tgtcaccctg
    13861 agatatcaca gtgacagttg tggaattatt tgtgtgaagc ggggtacccg ccggaacctc
    13921 cacctttacc acaatatcaa ttgaatcacc gcccgccacc ggcgcggtcg aggttatctg
    13981 gttagcgccg gaggcatcaa agaaagctac attccaggcc ccgctggagg tcagatcaaa
    14041 aatatcctgc aggaagccgt cattatagac tctcaggcta tagactgcgc tgtcaccggc
    14101 agggccgtat tcactctgga atgaggggcc caccgcaaca tcataatcag aaggatgacc
    14161 cacgtaaatg tcatctacaa accagtcatc gtagttgccc gacgtcgccg tgcacctgat
    14221 actcaagcgg aatccggcat gcatggcgcc cgcgggaagc tgtaattcaa cttcttcaaa
    14281 ttcggtcata tccggatcag cgccaagatg ccggtgcagc tcgatccagt tgctgtcagc
    14341 atcaaggtat tcaataatca ggtcgtcatt ggcgtccgga gattcagcat cgcccgtctg
    14401 ctgataggca tattctacca cgacattgga ctcgtctttg aggttgatca tctcggtgat
    14461 tatctgatct ccaccattgg gatcgccatt cagatcgacg ctgtatggcg agctcggttc
    14521 atctatcccg agcgtattga tctccgcgcc gtcccaagat tcccatttgg tattatcgaa
    14581 agtcgtggta gcgaaaatat cggtgaaggg gatttcccac ggttcaccgg tggagatcgt
    14641 cttcaagata gcggttttac tcagtccggg ctggctaaat gactcggcta cgatatcaac
    14701 cgaatcatat tctccttcaa gactcgaggg caccagaacc cttacataaa acggcaggct
    14761 gtcccccgac agcatgaggg tggtctggcc gatttgatta attctgggct catcccagat
    14821 cgatgtctgc cagacattgc cggccagggt taatgaa
//
LOCUS       DSEB01000257            3656 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002663, whole genome shotgun sequence.
ACCESSION   DSEB01000257 DSEB01000000
VERSION     DSEB01000257.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002663"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            55..1107
                     /locus_tag="ENO07_04740"
     CDS             55..1107
                     /locus_tag="ENO07_04740"
                     /inference="COORDINATES: protein motif:HMM:PF04464.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01308.1"
                     /translation="MNIQDYILPLFYKPAWHLSKLFGKNRWVGFYCADPLDYHMFEPI
                     KKHLPIPITYIAKNLKTGHFLKEINIPYKRYPAYPDVVIMARQTAYKFPVRKIVKIGF
                     DHGLYQFKRWTSSKYYNLFDVYFVSSENQVELAKERGITTTVAIGYPKLDKAFDGTYD
                     EQALEKLRRDLGLDPEKKTVIFSSTWDVAGLSALTRWIDHVHTLTDKYNTLLTAHPWT
                     DERLLVKLKSINGAVYLDEIDITRYLLIADVFVGDYNSLIGEFCALDKPIITFRTPDS
                     DRSIPAVKQLLSKISLQITDFDQVETAIENCLANPDQLSEGRRYGNMIMHHKLDGRAG
                     QRAAEIIMKLAHDIKK"
     gene            complement(1290..1832)
                     /locus_tag="ENO07_04745"
     CDS             complement(1290..1832)
                     /locus_tag="ENO07_04745"
                     /inference="COORDINATES: protein motif:HMM:PF01797.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01309.1"
                     /translation="MKLHHYDHDGRARFVTFCTHKKLQLLTNNCFRKKVTESIDWARH
                     EFGLKLLGYVIMPEHIHMVIVPKEESRLGQIIGELKRLSAKKILAEIKNDSLLGKLSV
                     ERDGVKRLAFWQRRCYDHNCRSDEFVWQKVNYCHYNPVKRGLVRSPEKWEWSSYRWYY
                     GYPNALLEMDVAGERSKPTC"
     gene            complement(2451..3335)
                     /locus_tag="ENO07_04750"
     CDS             complement(2451..3335)
                     /locus_tag="ENO07_04750"
                     /inference="COORDINATES: protein motif:HMM:PF00348.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDS01310.1"
                     /translation="MAEFKLDPVWEKDIAEKRELVNLWLERLLPPEDSVPVQLHEAMR
                     YSVMAGGKRFRPLLALYAYGLSGNDPVDIIKPACALEYIHTYSLIHDDLPCMDDDDLR
                     RGRPTVHKKFNETIAVLAGDALHAYAFELLASSGNSEIVSEVARAIGTYGMLGGQICD
                     ILAENKEINIEELKHIHTHKTGALIRASVRIGAILGGISGDDLEVLSGYGEKIGLAFQ
                     IVDDILDEVSDTETLGKTAGKDKETGKATYPRVVGLEKSRQIAEQLIASARDDLSRMN
                     IPVEVFHIFADLIISRQY"
     gene            complement(3337..3588)
                     /gene="xseB"
                     /locus_tag="ENO07_04755"
     CDS             complement(3337..3588)
                     /gene="xseB"
                     /locus_tag="ENO07_04755"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDS01311.1"
                     /translation="MAESKKAKDIDFKAGIERLEEIVENLESGEIDLEEALKVFEEGI
                     RISRSLSEKLEKAEKQIRKLVEESDGTLTTEVMEEIEEE"
BASE COUNT          964 a          923 c          748 g         1021 t
ORIGIN      
        1 ggccacagaa tatctgttgt cagaatagct ttaaatttct attatcatag ggacatgaat
       61 attcaggatt acatactccc ccttttttat aaaccggcct ggcatttatc caagctcttt
      121 ggcaagaaca gatgggtcgg cttttattgt gccgatccgc tggattacca tatgtttgag
      181 ccgataaaaa aacatctgcc aatccccata acatatattg ccaaaaacct aaaaaccggt
      241 catttcctga aagagatcaa tatcccctat aaacgctatc cggcttatcc cgatgttgtc
      301 ataatggccc gtcagaccgc ctataaattt ccggtcagga agatcgttaa gattggtttt
      361 gatcacggct tataccagtt caaacgctgg acctcgagca agtattacaa tctcttcgat
      421 gtgtattttg tttccagcga gaatcaggtg gaattggcca aagaacgcgg tatcaccacc
      481 acggtagcaa tcggctatcc gaaattggat aaggcttttg acggcaccta tgatgagcaa
      541 gcgcttgaaa agttaagaag ggatttggga cttgatcccg agaaaaaaac agtaattttc
      601 tcatctacat gggatgttgc cggactttcg gccctgacac gctggatcga ccatgtgcat
      661 acattgacag acaaatacaa caccctcctg acagcacatc cctggactga tgagcgtctt
      721 cttgtgaaat taaagagtat aaacggagcg gtctatcttg atgaaatcga cattaccaga
      781 tatttgctga ttgctgatgt tttcgtgggg gattacaatt cgttgatcgg cgaattttgc
      841 gccctggata aaccgatcat aactttcagg actcccgaca gcgacaggtc gattccggca
      901 gtcaaacagc tgctctccaa aatcagcctt cagatcaccg actttgacca ggtcgaaacg
      961 gctattgaaa actgcctggc caatcccgat cagctgagcg aggggcgacg ttacggcaac
     1021 atgatcatgc accacaaatt ggatggccgt gccggacaac gcgccgcgga gattatcatg
     1081 aagctggccc atgatattaa gaaataactc taaaatatta gaattcgaag tagtgcatag
     1141 tcgctgcgca atccacactt tgtaccccac ccaccgggtt ggaaatttcg atcatacatg
     1201 gtaccccaca tgaaatgtgg gtttagccgg gcatactgct atatcttatt ttgaatagca
     1261 acacagcagg tgaaattttc ttcgcccacc taacaggtgg gtttactccg ctcacctgcg
     1321 acatccattt caagaagtgc attggggtaa ccatagtacc acctgtagct gctccactcc
     1381 catttttctg ggcttctaac caaacctctt ttcactggat tataatggca ataattgact
     1441 ttttgccaca caaattcatc actacggcag ttatggtcat agcatcttct ctgccagaat
     1501 gccaaacgct tcactccatc gcgttccaca ctgagcttgc ctagcagcga atcatttttt
     1561 atttcagcca atatcttttt tgcggataaa cgctttaatt cgccaataat ttggccaagt
     1621 ctggattcct cttttggcac tattaccata tgtatgtgtt caggcataat tacatatccg
     1681 agaagtttca agccaaattc atgccttgcc caatcgatag attcagtcac tttttttcta
     1741 aagcaattat ttgtcaataa ttgcaatttc ttatgagtac agaatgtgac aaatctagct
     1801 ctgccatcat ggtcgtagtg atgcagcttc atactgcaaa aaatagtatc gccaatatat
     1861 aatgtcaatc ataaatcgca gcatagcagg tgggcgattt aaacccacct gatgggtggg
     1921 gtacattgta tttttgtgta ttgcacgcaa aaacagagaa taaaatggta attattagta
     1981 tcaatccaca gccaatagat tggcttttgg aagattttcc tttttgacat gaattcatcc
     2041 gggtttcctt tccaattaca ctttgtaccc cacccagcgg gtgggaaatt tcggtcatcc
     2101 tgctatgcct gatcttgaaa agcagcacag caggtgaaat tttcttcgcc cacctgttag
     2161 gtggggtaca aatgtgggcg atttaaaccc acccgatggg tggggtacat tgttaggtgg
     2221 gatacagtgt gtttaatcga tcatacaccg gtaccccaca tgaaatgtgg gaaattccgt
     2281 catactgcta tgtcaatctt cagtacaatt tcgtctaact tccacccttg ctgtattgac
     2341 gaccggcgta ttcgagtgtc aattgaaaaa taaatacatg ctctataatg tcaattataa
     2401 tcttgcgcaa ttctttgatc cgggttaatc cctcgggaaa cctcttttat ctaatactgc
     2461 cgcgaaatta tcaaatctgc gaagatgtgg aagacttcga ccgggatgtt cattcttgaa
     2521 aggtcatccc tggctgaagc aatcaactgc tctgcaatct gccgcgattt ttccagtcca
     2581 acgacacgcg ggtaggtggc cttgccggtt tccttgtcct taccggcagt cttgcccaga
     2641 gtctcggtgt cggagacctc gtccagaata tcatcgacaa tctgaaaggc cagcccgatc
     2701 ttctcaccat aaccggaaag aacctcgaga tcatctccac taataccacc cagaatcgcc
     2761 ccaattcgaa ctgaggctct tatgagcgcg ccggtcttat gtgtatgtat atgtttcagc
     2821 tcttcgatat ttatctcttt attttccgcc aggatgtcgc aaatctgtcc ccccagcata
     2881 ccataggttc cgatcgcccg cgcgacctca cttacaatct ccgaattacc ggatgaggcc
     2941 agaagctcga aggcgtaagc atgcagagca tcccctgcca gcaccgcaat agtctcgttg
     3001 aatttcttat gcaccgtggg acgacctcgc ctcagatcat catcatccat acatggcaga
     3061 tcatcatgaa tcaatgaata tgtatggata tattccaacg cacaggcagg tttgatgata
     3121 tcgaccggat cattgccgga gagcccgtaa gcatacaggg ccagcagagg acgaaaacgt
     3181 ttgccgccgg ccatcacgct gtagcgcatg gcctcatgaa gctggaccgg gaccgaatcc
     3241 tcgggcggaa gcaatctctc cagccagaga ttgaccagct cccgtttttc agcgatatcc
     3301 ttttcccaga ccggatcgag cttgaactct gccatactac tcttcttcaa tttcttccat
     3361 cacctcggtc gtcagcgtgc cgtccgattc ctcgaccagc ttgcgtatct gcttttcggc
     3421 tttttccagc ttctcggaaa gactgcgtga aatccgtatg ccctcctcga ataccttcaa
     3481 cgcctcctca agatcgatct cgccgctttc aagattctcg acaatctcct caaggcgctc
     3541 gattccggct ttgaaatcta tatctttcgc ttttttcgat tcagccataa ataaactccg
     3601 ggttcaggca taaactaatt tctttttgcc ccaagtcaat cgctttcttt ctcgac
//
LOCUS       DSEB01000258            1506 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1018952, whole genome shotgun sequence.
ACCESSION   DSEB01000258 DSEB01000000
VERSION     DSEB01000258.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1018952"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..300)
                     /locus_tag="ENO07_04760"
     CDS             complement(<1..300)
                     /locus_tag="ENO07_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photolyase"
                     /protein_id="HDS01312.1"
                     /translation="MSISIDDRRIRVLAKGEHRKGPVIYWMSRDQRVEDNWALLFAQQ
                     LAVEYKAPLAVIFCMVPEYLDATMRQYGFMIEGVQEVEKALQKKNIPFFLLLGEPE"
     gene            complement(284..1258)
                     /locus_tag="ENO07_04765"
     CDS             complement(284..1258)
                     /locus_tag="ENO07_04765"
                     /inference="COORDINATES: protein
                     motif:HMM:PF02310.17,HMM:PF13411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDS01313.1"
                     /translation="MEHKHPIKVVEKRTGLSSHVMRIWERRYSAVTPERTDSNRRMYT
                     DGEIERLILLRKATQAGESIGQIARLSDPQLKMLVESSASNFNIDPRYSSGGGNGESY
                     HEPRLYLKECLDAIVDLDSEKLEKILLRASSELTQPEMMEKVLEPLMYKIGDLWREGT
                     LRVVHEHLASAVVRSFLGNMVGALKVDESAPKIIVTTPPGQIHEFGALMVTIAASSAG
                     WHSIYLGPELPAEEIANAVKNIEARAVALSIVYPPDDPRLGPELRRLRQILDDEVVLL
                     VGGRSSGAYNNVLEDLGIEAMQRLSDLHKHLEKIRSKSNGVKTDVDFN"
BASE COUNT          391 a          399 c          331 g          385 t
ORIGIN      
        1 ctccggctca ccgagaagca ggaagaacgg gatgttcttc ttctgcagag ctttttcaac
       61 ctcctgcaca ccttctatca taaagccgta ctggcgcatg gtggcatcca gatattccgg
      121 gaccatgcag aatattaccg ccagcggggc cttatattcc acagccagtt gttgggcgaa
      181 aagaagggcc cagttatcct caacacgctg atcacggctc atccagtaaa ttaccggacc
      241 cttcctgtgt tcgcccttcg ccaggacacg gattcgtctg tcatcaattg aaatcgacat
      301 cagtcttcac cccattggat ttactcctga tcttttccag gtgtttgtgc aggtccgaaa
      361 gacgctgcat cgcttcaata cccaggtctt ccaatacatt attataagca ccggaagatc
      421 gtccaccaac taaaagaaca acctcgtcat cgagaatctg gcgcagtctg cgaagttccg
      481 gtcccagacg aggatcatcc ggaggataga caatacttaa tgccaccgcc cgggcctcaa
      541 tatttttgac cgcattggca atttcctcgg ccggaagttc cggtccgaga tagatcgagt
      601 gccatcccgc agaggaggcc gcgattgtta ccatcaatgc tccgaactca tgaatctggc
      661 cgggcggtgt ggtaacaatg atcttcggag cggattcatc aactttaagc gccccaacca
      721 tatttcccag gaacgagcgc actactgcag aggccagatg ctcatgtacc acgcgcaatg
      781 tgccctcgcg ccagagatcg ccgatcttgt acatcagggg ctccagcact ttttccatca
      841 tctccggctg ggtcaattct gatgaggccc tgagcagtat tttctcgagc ttctccgagt
      901 caagatcaac tatcgcatcc aaacattcct tcaggtagag cctcggctca tgataagatt
      961 ctccattgcc gcccccactc gaatatcggg ggtcgatatt gaagttagag gcgctcgact
     1021 ccaccagcat tttcagttgc gggtccgaga gcctggcaat ctggccgata ctctcacccg
     1081 cctgcgtggc tttgcgcaga aggatcagcc tttcaatctc tccgtcggta tacattctgc
     1141 ggttcgaatc tgtacgttca ggggtgacag cgctgtaacg ccgttcccat atcctcatca
     1201 catgagagga aagtcccgtt ctcttttcca cgaccttgat tggatgttta tgttccataa
     1261 ttcaccttgc aaactaatgt tctaatatcg tctaagattg ataacacaag aatttgacaa
     1321 aaagttcgat gaaaagcata attattgagg aatttcatca aaataagctc gatagtcaaa
     1381 acgatttcat aaatctacaa caataactta ttgcggtaca tcataaaagg gagaattatg
     1441 agtatgttta gacatctttg gaacattatc aagacattat aggttgcaga agtcgaaagt
     1501 tgaaaa
//
LOCUS       DSEB01000259            3341 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003245, whole genome shotgun sequence.
ACCESSION   DSEB01000259 DSEB01000000
VERSION     DSEB01000259.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003245"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            198..881
                     /locus_tag="ENO07_04770"
     CDS             198..881
                     /locus_tag="ENO07_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01314.1"
                     /translation="MGELDLGYVFGDDFKFENSAFFLRGKTYIERTDISGMVIGFREN
                     LLLGFDLTRYIGGAGFWVEGAYVFSGALSHDGFDEDESYFRGSVGLDYSFRSGTYSFI
                     EYHYNQAGSNDPDDYYSLLNQTAYSEGSVYFLGEHYLAPGFSYQITPLITGYAEAIIN
                     LGDPSLLAAPSVEYNIEENIYLSAGGFIGIGEGFREREAIPIVPPVVPQSEFGSYGDI
                     YFVSFKIYY"
     gene            975..2363
                     /locus_tag="ENO07_04775"
     CDS             975..2363
                     /locus_tag="ENO07_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HDS01315.1"
                     /translation="MKIVKRVESVINPKILKPGSVIYTSGNAATPQVLLEQLARDMDI
                     KHVALFCVLPLGERIRPLFSKERCQSITHRVIFNSLLTREAVNQGWAKYHPMHLSEIP
                     RYAPEPDGPNVVLCSVAGPDNGGNYSLGTTVEAVLAAIRSARDKGGVVIAERNARMPF
                     VGGTAIPQRYIDYLIDADYPLPVSPVQKPDKKAMRIGEIIAALYIKDGSGKTPGSTLQ
                     YGIGEVPEAVTSAILKKNCQHLGIHTELFADAMMRLVKKGLVTNKWKPRINFSVSSIF
                     LAKDQKGYEWLHFNSAVQSRPSDYTNNAFVIAQQPNMVAINSAIGVDLHGNIWADSLD
                     ARRIYSGIGGQSDFIRGAQYSPGGVAIIAMKSVTRDGRSKIVDRCPAGITTTAIPADR
                     VILVTENGAFDPKSLSMGERAVGIAHLATDEERERLLKTIRDDPAFHKPDLTMHKGVP
                     GFTPYKTAIDRQ"
     gene            complement(2384..>3341)
                     /locus_tag="ENO07_04780"
     CDS             complement(2384..>3341)
                     /locus_tag="ENO07_04780"
                     /inference="COORDINATES: protein motif:HMM:PF02518.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDS01316.1"
                     /translation="SDAALANIFVRNPSGGCSYNIDKAPWQSVFSRYPILAFLAGFIP
                     LSIIIAVTWIILLKFRQKNKTIAQQRDSLDNTLKELRQTQQKLIEAEKYKQAKDMAGG
                     FAHEIRNALFPAKASIKKIESDLRKSDQADSGIPKLTLLASTAISKAIELTNLISQYT
                     RLDSIYQPENVNIHSIIEEVLETNELRLKENSVEINLEGSDQVCIESNRRQFFSVINN
                     LLLNSIDAMKDKSERRIDISWSHNESSVYLKFRDSGGGIPLELQESVFNAFRSYKKEK
                     GIGLGLAIVKRVVEMYGGTISVTSQPGTGTSFNLKLKSSKCP"
BASE COUNT          806 a          805 c          878 g          852 t
ORIGIN      
        1 aatcagaatc tcgaccgggc atatttgagc ctgtcatttc cgaagtttga cctgtatttc
       61 ggccgccagc tatagcctgg ggttcggccc atgtaataaa tcccactgat gtcctggcgc
      121 ctttcccata taatgagctc gatatcgaaa atcgcattgg cgttgatgct ctgcggattc
      181 gctatcccct gggctttatg ggcgaacttg acctgggata tgttttcgga gatgatttta
      241 aattcgagaa tagcgccttc ttcttgcggg gcaaaacata tatagagcgc acggatattt
      301 ccggaatggt tataggattc cgggaaaatc tcctgctggg atttgatctg acgcgctata
      361 taggcggggc aggtttctgg gttgagggtg cgtatgtctt ttccggcgcc ttgtcacatg
      421 atggatttga tgaagacgaa tcatacttcc gcggctctgt cggcctggac tatagtttta
      481 gaagcggaac ctacagcttt atcgaatatc attataacca ggccggcagt aatgaccccg
      541 atgattatta cagcctgctg aatcaaaccg cttatagcga gggctcggta tattttctgg
      601 gagaacatta tctggcaccc ggattttcat accagattac gcctcttata acaggctacg
      661 cggaggcgat catcaatctt ggcgatccgt cactgctggc agcgcccagc gttgagtata
      721 atatcgaaga gaatatatac ctgagtgcgg gcggttttat cgggataggc gagggcttca
      781 gggagagaga ggcaataccg attgtacccc cggttgtccc tcaaagcgaa ttcggaagct
      841 atggggatat ttattttgtc tcgtttaaga tatattattg aaagtgttta tcttgatccg
      901 gacttaaggg atgcaaacct gatttgatat aaaattgctg gaaattattg ttgtaacata
      961 ttatctataa cctcatgaaa attgtcaaaa gagttgaaag cgtaataaat ccgaagatcc
     1021 tgaagcccgg aagcgtgatc tatacttccg gtaatgccgc tactccgcag gtcctcctgg
     1081 agcagcttgc cagggatatg gatatcaagc atgtcgcttt attctgtgtg ctgccgcttg
     1141 gtgagaggat caggcccctg ttttccaaag agcgctgtca gagtataacc cacagggtga
     1201 tattcaacag cctcctgacc agggaagcgg tcaatcaggg ctgggccaaa tatcatccca
     1261 tgcatctttc cgagataccg cgctatgcgc ccgagcctga cggtcccaac gtggttctgt
     1321 gttcggtggc cggacctgat aacggcggca attacagcct gggtacgacg gtggaggcgg
     1381 tcctggcggc aatccgctcc gcccgcgaca agggcggtgt ggtgatagcc gagcgcaacg
     1441 cccgcatgcc ctttgtaggt ggcaccgcaa ttcctcagcg atatatagac tatttgattg
     1501 atgcggatta tcccctgccg gtaagcccgg ttcaaaaacc cgacaagaag gccatgagaa
     1561 tcggtgaaat tattgccgcc ctgtatatca aagacggcag cggcaagacg cccggttcca
     1621 ccctccagta tggtatcggg gaagtgcctg aagcggttac ctcggccatt ctcaaaaaaa
     1681 actgccagca cctggggata cacacggagt tgtttgccga tgctatgatg cggctggtga
     1741 agaagggtct ggtgaccaat aagtggaagc cgcggatcaa cttctcagtc tcctcgattt
     1801 tcctggccaa ggatcagaaa ggttacgagt ggcttcattt caatagtgcg gtgcaaagcc
     1861 ggccctcgga ttacaccaat aatgcttttg tgatcgccca gcagcccaat atggtggcga
     1921 tcaattcggc tatcggggtc gacctgcacg gtaatatctg ggccgattcg ctggatgccc
     1981 gcaggattta cagcgggatc ggcggccaaa gcgactttat caggggggcg cagtattctc
     2041 ccggaggggt tgccataatc gctatgaaat cagtcacccg cgacggcagg tcgaaaatcg
     2101 ttgacagatg cccggccggg atcactacaa ctgcaatacc agccgatcgt gttatactgg
     2161 tgacggagaa tggcgccttt gatcccaaga gcctcagtat gggtgaacgg gctgtgggga
     2221 ttgcacatct tgccactgac gaagaacgag agaggctcct taagacgatc agggacgatc
     2281 ccgctttcca taagcccgac ctgaccatgc acaagggagt ccccggattt acaccttaca
     2341 aaacagccat cgataggcag taatagctct atcagtgaaa agactacggg cacttcgaag
     2401 attttagctt cagattgaag ctcgtgcccg tcccgggttg gctcgtgacc gaaattgtgc
     2461 ccccatacat ctcgacaacg cgcttgacta ttgccaggcc caggccgatt cccttttctt
     2521 ttttatagga tcggaaggca ttgaacaccg attcctgaag ttcgagcggg atgccgccgc
     2581 cgctatcgcg aaatttaaga tataccgaac tctcattatg ggaccaggaa atatcaatcc
     2641 ttcgctcgga tttatccttc atggcatcta tgctgtttag aagcagattg tttatgaccg
     2701 aaaagaactg ccggcggttc gactcgatgc agacctgatc cgaaccctca agattgattt
     2761 caacagaatt ctctttgagc cgcaactcgt tggtctccag cacttcctct ataatcgagt
     2821 gaatatttac attctcgggc tggtaaatcg agtcaagcct tgtatattga gaaatcagat
     2881 tggtcagctc gatagctttg ctgatggcgg tactggcaag cagggtgagc tttggaatac
     2941 ccgaatctgc ctgatcgctt ttgcgaagat cgctttcgat ctttttgata gaggccttgg
     3001 cggggaaaag ggcattgcga atctcatgtg caaagccgcc agccatgtcc ttggcctgtt
     3061 tgtatttttc ggcttcaatc agtttctgct gtgtttgcct caattctttc aaggtattgt
     3121 caagcgaatc tcgctgctgt gcaatggtct tattcttctg cctgaatttc aagagaataa
     3181 tccaggtaac cgcgatgatt atcgataagg gtatgaatcc ggccaggaag gccaggatcg
     3241 ggtagcggga gaaaacgctt tgccagggag ccttgtcgat attgtacgag cagccgccgg
     3301 atggattccg tacaaagata ttcgccagcg ccgcatcaga c
//
LOCUS       DSEB01000260            1598 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1016642, whole genome shotgun sequence.
ACCESSION   DSEB01000260 DSEB01000000
VERSION     DSEB01000260.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1016642"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            741..1598
                     /locus_tag="ENO07_04785"
     CDS             741..1598
                     /locus_tag="ENO07_04785"
                     /inference="COORDINATES: protein motif:HMM:PF00877.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01317.1"
                     /translation="MEMSITMGGHSNPPIFYVGQRFKIYIIDIMAKKVVKVVVPVADI
                     WGKPAFESSRISQAVFGQKAEILKRKQKYFYIQCEDGYRGWVNQSHVVKLKTGSPLYA
                     QSVVRVPVAEIYRMPKSRTVSGRLSFGSRIEYDQKLSGMLHLVDVEGWISEADLRLKK
                     PPRISARAIVTTLKMFLGIPYLWGGKSGFGLDCSGLVQIVYGFYGYDLPRDSKDQRNK
                     GRRVSRKNLKPGDLLFSPGHVAVYLGKGSIIHSSLRAGGVKIESIDSKSKLYREDIEK
                     KIIEMRRVI"
BASE COUNT          407 a          340 c          429 g          422 t
ORIGIN      
        1 atcatcaatt atatctttct cgatgggagc ggtccgctga gcaatgactg cattcatctc
       61 gagaaaaccg gcagttgcct tgaacgggac ctggcgcccg attcgggcta tgtttatatc
      121 gaagtactcg gtaatgatct gcacgttcat catatggacg cttactacca gtgctgcctg
      181 gaattcttca tggagtacag cttcgaagaa agcattataa cagcacagga aaccgacata
      241 ggcgatccat gtgactgtat ctgctacttc gattccctcc gttcggttta ttatgatctc
      301 gatgacggcc agtacactgt gatagtcctg ggcatatatg gtgacaccat ggctgtcgag
      361 gaaattgtcg tggatggtga ttacggcctc tcggggcaca gttcatacgg gtgcctcaag
      421 ggagattttg acggcgatcc tccgataata gattatactt ttgaaaacgg cattttgatc
      481 atgagtcacg gcaatgccta tttcaattgc ggcggcaaga tcgtggccgc gtttgaacag
      541 gcctccgata ccctgaggtt tttcgagatt aatgtctcgg acctgtgggc ttattgcatg
      601 tgctattttg ccgtcagcgc gagtgtggac ggtctggcgc ccggggtata tacagccgag
      661 gtctatgctc gcgagccgat ggatgctccc gatgcgcttg ttgaccgaag ggaaattatt
      721 ttgccataga agcatttcat gtggaaatga gcattacaat gggcggacat tctaatccgc
      781 ccatttttta tgttggacaa aggtttaaaa tatatattat agatatcatg gcaaagaagg
      841 tcgtaaaagt cgtggttccc gtagcggata tctggggcaa gccggccttt gagtcctccc
      901 gcataagcca ggcggtcttc gggcaaaagg ctgaaatcct caagcgaaaa cagaaatatt
      961 tttatattca atgcgaggat ggatacaggg gctgggtaaa tcagtcccat gtggtcaaat
     1021 tgaaaacagg cagtccgctg tatgctcaat cggttgtgcg tgtaccggtg gctgagattt
     1081 accgtatgcc caagtcacga accgtgtccg ggagattatc cttcgggtca agaatagagt
     1141 atgaccagaa gctgtcgggg atgcttcatc tggttgatgt tgagggctgg atcagcgaag
     1201 ctgatttaag attgaaaaag cctccgcgca tttctgccag agcaatagtc acgacgttaa
     1261 agatgtttct gggcattccc tatctctggg gagggaaatc cggattcggt ctggactgct
     1321 caggcttggt gcagattgtt tatgggtttt acggttatga ccttccccgt gactcgaaag
     1381 accagagaaa taagggcaga agggtctccc gcaagaactt aaaaccgggt gatctgctat
     1441 tctcaccggg tcatgtggcc gtttatctgg gaaaagggtc gatcattcat tccagcctga
     1501 gagcaggcgg tgtcaaaatt gagtctatcg acagtaagtc aaaactctac cgtgaagaca
     1561 tagaaaaaaa aataatagaa atgcgcaggg ttatatga
//
LOCUS       DSEB01000261            3376 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003162, whole genome shotgun sequence.
ACCESSION   DSEB01000261 DSEB01000000
VERSION     DSEB01000261.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003162"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..417)
                     /locus_tag="ENO07_04790"
     CDS             complement(<1..417)
                     /locus_tag="ENO07_04790"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822466.1"
                     /note="Catalyzes the reversible oxidation of malate to
                     oxaloacetate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDS01318.1"
                     /translation="MRKKVSVIGAGNVGASCAMYLAARNICDVVLVDIIEDMPAGKAL
                     DLAQAGPLVGYDVMVHGTNDYADIKDSDMVVVTSGLARKPGMSREDLLKKNAEIIKSV
                     TENIVKYAPNAYILMVANPLDLMTYHAAQVSGFPKNR"
     gene            complement(436..1659)
                     /gene="larA"
                     /locus_tag="ENO07_04795"
     CDS             complement(436..1659)
                     /gene="larA"
                     /locus_tag="ENO07_04795"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HDS01319.1"
                     /translation="MKVPLRYADEEYELDVPDKNLAGVIEPSKTEPVDLEDAIEKNRL
                     PERFNSGSLRILIIINDQYRSTPSHLILNRILPSLLDRHEVRIIVATGLHDEPAMEEY
                     EKLIGENFEKMDRKFAWSNSRDYVSFRNVGFWPDGGEVYIHNYYFWADDVIIIGSVEP
                     HYFAGFTGGIKSIVPGLSYYRTIEHNHNKAVSVKSQPGVTEGNPVWEELWKTLDLIDE
                     KRIFSYQLVQDSERNIIGLFSGSIRESYFRAVKFCQDIYFKNTPQKCDLLVAEHSPPL
                     DRNLYQLQKCFENTKSGVKDGGTLLMLSGCRDGIGTRDFYDLAEKFPDPNDLLNQEIK
                     RCDLGIHKLYRTAILTNRVNLCLMSFLQEEAVRRIYIEPVADANELVRSRVAADGNLK
                     ILVVKDAGHTVINTE"
     gene            complement(1656..2072)
                     /locus_tag="ENO07_04800"
     CDS             complement(1656..2072)
                     /locus_tag="ENO07_04800"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020412168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDS01320.1"
                     /translation="MTTQKTLTIIKPDAVGKNALGEIIHRFELAGFRIVAMTMEHLTR
                     ERAEGFYQVHSERPFFSELVEFMTSGPCVPMVLEKENAIADLRILIGVTDSAQAAPGT
                     VRQNFGTDIQCNAVHASDSVENARFEVNYFFPDIKV"
     gene            complement(2082..2672)
                     /locus_tag="ENO07_04805"
     CDS             complement(2082..2672)
                     /locus_tag="ENO07_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_952521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     gamma"
                     /protein_id="HDS01321.1"
                     /translation="MSKNKNHQAQSDRYEIRLSGSGGQGLILAGVMLAEAMGVEDGKN
                     VVQTQSYGPEARGGASRADVVVSSDTIYYPKPMGLDLLLALTQEACDTYFSALRENGI
                     LIIDSDLVHQPPTPDCYSFPFTMLAKNEIGHVMVANVISLGAIVEITRLASKDSIKTV
                     VKSRAPVGTEEKNLKALELGFKIAKEKMKSTRTAAV"
     gene            complement(2686..>3376)
                     /locus_tag="ENO07_04810"
     CDS             complement(2686..>3376)
                     /locus_tag="ENO07_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159425.1"
                     /note="catalyzes the coenzyme A-dependent formation of
                     succinyl-CoA from 2-oxoglutarate and ferredoxin; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HDS01322.1"
                     /translation="DDIAVLSGIGCSSRLPVYVDFNTLHTTHGRAIAFATGVKVAKPK
                     LNVIVITGDGDCLAIGGNHFIHACRRNINITTILVNNHIYGMTGGQFSPTTPYTKRGS
                     TAPYGNLEHPFDAAKLAIAAGASYVARGTVYHVGQLTRLIADGISNNGFSLIDALSNC
                     HTYYGRWNKEGDPVKMIEWFKSHVVPAATADKMPPEKKEGKWLTGVLHQEDKPEYTEN
                     YARLRAELKKK"
BASE COUNT          797 a          907 c          797 g          875 t
ORIGIN      
        1 acggtttttg gggaagcccg aaacctgggc cgcatgatag gtcatcagat cgagcggatt
       61 agcgaccatc agaatgtaag cgttgggggc atacttgact atattctccg tcaccgattt
      121 tatgatttct gcattcttct tgagcaggtc ctcgcgggac attcccggct tgcgggccag
      181 accagaggtg accacaacca tgtcggaatc cttgatatcc gcgtaatcgt tggtaccatg
      241 aaccataacg tcataaccca ccaggggccc ggcctgggcc agatccagag cttttccggc
      301 aggcatatct tcgataatat caaccagcac cacatcgcag atattgcggg ccgccagata
      361 catcgcacat gaggcaccaa cattaccggc gcctattaca gaaaccttct tgcgcatctt
      421 aatagtcctc ctactttatt cagtatttat caccgtatga ccggcatcct taactactag
      481 tattttcaaa ttgccatcag ccgcaactct tgacctgacc aactcgttgg cgtcagcaac
      541 cggctcgata taaatccttc tgacagcctc ctcttgaaga aatgacatta aacagagatt
      601 tacccgattt gtcaatatcg cagtgcgata aagtttatga attcccaaat cacacctctt
      661 tatctcctga ttcagaaggt catttggatc aggaaatttc tcggccagat catagaaatc
      721 acgcgtcccg ataccgtccc ggcagcccga gagcatcaaa agggttccgc cgtctttgac
      781 gcctgattta gtattttcaa aacatttctg gagctggtag agatttctgt ccaggggcgg
      841 tgagtgttcg gccaccagca gatcgcactt ctgaggggtg tttttgaaat aaatatcctg
      901 gcagaacttc accgcccgaa aatagctctc cctgatcgag cccgaaaata aaccaatgat
      961 atttcgctcg gagtcctgca cgagctgata cgagaaaatc cgcttttcgt ctatcaagtc
     1021 cagggtcttc caaagctcct cccagaccgg attaccctcg gttacccccg gctgcgactt
     1081 tactgagacg gccttattat gattgtgctc gatggtcctg taatagctga gtccgggtac
     1141 aatcgatttt attcctcccg taaaacctgc gaagtaatgc ggttcgactg atcctataat
     1201 gatgacatca tcggcccaga agtaataatt atggatataa acctctcctc catcgggcca
     1261 aaatccgaca ttccgaaaac tgacatagtc gcgggagttg gaccaggcga atttcctgtc
     1321 cattttctcg aagttctcac cgatcaattt ctcatattct tccatcgcgg gttcgtcatg
     1381 gaggccggtt gcaactataa tcctcacttc atgcctatcc agcagagaag gcagtatgcg
     1441 gtttagaatc agatgtgaag gggtggagcg atactgatca ttaatgatta tcaaaatacg
     1501 aaggctgccg gaattgaatc tttccggcag cctgttcttt tctatcgcat cttcgagatc
     1561 gacgggctcg gtttttgagg gctcgattac gccggcaaga tttttgtccg gcacatcaag
     1621 ctcatattcc tcgtccgcat atcgaagggg aactttcata ctttaatatc tgggaaaaaa
     1681 taattaacct cgaacctggc attttccaca gaatctgaag catgtacggc gttgcactga
     1741 atatcggtcc cgaaattctg cctgaccgtt cccggggcgg cctgggccga atcggtgacg
     1801 ccgatcagga tacgcagatc agcaatcgca ttttcctttt ccagcaccat cggcacgcag
     1861 gggcccgagg tcatgaactc gaccagttca gagaaaaacg gcctctcgct gtgcacctgg
     1921 tagaatcctt cggcacgttc cctggtcagg tgttccatgg tcatggccac gatcctgaaa
     1981 ccggccagct caaagcggtg tatgatctcg ccgagtgcat ttttgccgac cgcatcgggc
     2041 ttgatgatcg tcagggtctt ctgggttgtc ataatcgctt tttacaccgc agccgttctt
     2101 gtggatttca ttttctcttt ggcaatcttg aagcccagtt caagggcttt gagatttttc
     2161 tcctcggttc cgaccggggc cctggatttt accactgtct tgatagaatc tttggaggcc
     2221 agcctggtaa tctctacgat cgctcccagt gaaatcacat tggcaaccat cacatgtcca
     2281 atctcgttct tggccagcat ggtgaatgga aacgaatagc aatccggcgt gggcggctga
     2341 tggacaagat cgctgtcaat aatcaatata ccgttctcgc gcaaagccga gaaatacgta
     2401 tcgcaggctt cctgggtcaa agccagaagc aggtccaggc ccatcggctt gggatagtat
     2461 attgtatcgg atgagacgac cacatcggct cgtgaagccc ctccacgggc ctccggacca
     2521 taggactggg tctgcacaac attcttacca tcctcgaccc ccatagcctc ggccagcatg
     2581 actccagcca gaatcagccc ctgccctcct gagcccgaga gccttatctc ataccggtct
     2641 gactgtgcct ggtgattttt atttttggac atctataact cccgtttatt tctttttaag
     2701 ctcggctcta agcctggcat agttttcggt atattccggc ttatcctcct gatgcagaac
     2761 cccggtaagc cacttgcctt ctttcttttc gggcggcatt ttatcggcgg tggcagcggg
     2821 caccacatgg cttttaaacc attctatcat cttcaccggg tcgccctcct tattccagcg
     2881 gccataataa gtatgacagt ttgaaagggc atctatgagc gagaagccgt tgttggagat
     2941 gccatcggca atcagcctgg tcaattgtcc cacatgatag acagtgccgc gcgccacata
     3001 cgatgctcca gcggctatgg ccagtttggc agcatcgaag ggatgttcca gattgccgta
     3061 gggtgcggtg gaaccgcgct tggtataggg cgtggtgggc gagaactgcc cgccggtcat
     3121 gccatagata tggttgttca ccagaatcgt cgtgatattt atattgcggc ggcaggcatg
     3181 gataaagtga ttccctccaa tcgccaggca gtcgccatcg ccggtaatca cgatcacgtt
     3241 cagtttgggc ttagccactt tcaccccggt cgcaaatgcg atagcccggc catgggttgt
     3301 atgcagagta ttgaaatcaa cataaaccgg aagccgtgaa gagcatccaa ttccagatag
     3361 caccgcgata tcatcc
//
LOCUS       DSEB01000262            4104 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002061, whole genome shotgun sequence.
ACCESSION   DSEB01000262 DSEB01000000
VERSION     DSEB01000262.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002061"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..726
                     /locus_tag="ENO07_04815"
     CDS             <1..726
                     /locus_tag="ENO07_04815"
                     /inference="COORDINATES: protein motif:HMM:PF12801.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDS01323.1"
                     /translation="DYFIPLHPSPMCAVTNLFMFKFTMGKFFPAFLVLFLAMIIPSLF
                     GRKLFCGWVCPLGAYQEVINKIPHKFKWKNFNFAIFNTVRFSLLILFILTFFAVKDHV
                     AYLAENVGADTSSGIWTAFSAYTVYQPINFFELLHWNIDTIFLIMMPILVIASLIIYR
                     PFCYLICPIGALTWLCEKIAPGRVRVDHNKCTLCGNCEEQAPCPTIKPMLEENSRVLP
                     DCTSCGDCLGSCEEDAIKFSFKR"
     gene            889..3876
                     /locus_tag="ENO07_04820"
     CDS             889..3876
                     /locus_tag="ENO07_04820"
                     /inference="COORDINATES: protein
                     motif:HMM:PF13360.4,HMM:PF13517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01324.1"
                     /translation="MFKLNRREKPSGMIAFWAFFWLMFLALSLCAEDVGFWPPGQKSS
                     DYSSSPIWTYGGDLPVGHIEVADLNGDNLKDIIAAEYNPDNYSDSALVYALDGFTGDT
                     LWTFWFDDGVRVLISGDINLDNVPDIIGGAALGIDIAVDGHVRAIDGATGDEIWSFAT
                     GGSITDLAIGNFNNSGNLDVAVVGYDDYVYAVDGSTGDDIWSRYLTSVYINAVAVGDV
                     NEDGIDDVGYAHEKLTGFDNYMGVLNGVDGSVIWEQINTYFGTDLLIDDIDDDGLLEA
                     VFGLHFDDDHGEIQVRNALTGGLEWSYDFGPMNSTYGEFYLNSIDIDDDKDKDLIVGN
                     YAAQKKLYIFDGDNNIPAVVSPEVPSYIYQTAAGDVDNDGFLDIIAASSDRVQIFNSA
                     TGNLMWYFGVNGNINDVACADFDGDLWTDIAAGGGANFSPSPLQTVWALKTIQTPLLW
                     QYNFGRYGNALTVVDLDQDGHDDVVTVASLDDRATAIDGKTGNSILWSWTGTENLYAV
                     THGDFDNDGDQDIAVAGYDQTVTALDGLSGEVLWQFTTPTAQIYRSCLVSTDLNGDGA
                     DDVIAGSDDSFVYAINGLNGMELWNYPPGDALEEVALGQMNGTGPLDVVCGLTNSITG
                     SRVVVLDGSDGSWLWQYNCNYRVEYVEVFDVNGDEVPDVAAGVTYTDPTLYMLDGATH
                     TPLWTIPVDINPNNYGLAHGDVNGDKDPDVIVAGTSATNKVFVYDGPTGALLWDFTIG
                     APVLCVLGYDVNGDGDKEVIAGGDDDKIYVIDGDGTEMFSYYCAERVKHIQVGDISGN
                     GDPNIACVTFGSSGIAYAFKSLVPEPNVPPYTPTSPDPEDGAIGISVTASLSWAGGDP
                     NANDQVHYDVYFGTDEPLPLVSDDQLVPSYDPPGDLEYGTEYLWQIIAFDEAEESTAG
                     PIWSFKTEYAVMCGDCNDDLSVNVSDAVFIINYVFIGGTAPDPLCVGDTNADGSVNVS
                     DAVAIINYVFVGGDAPDPNCCGK"
BASE COUNT          977 a          930 c         1095 g         1102 t
ORIGIN      
        1 gattatttca ttcccctgca tcccagcccg atgtgtgcgg tcaccaatct ttttatgttc
       61 aagttcacaa tgggcaagtt cttccccgca tttctggtat tattcctggc gatgattata
      121 cccagcctct tcgggcgcaa gctgttctgc ggatgggttt gcccgctggg agcttatcaa
      181 gaggttatca ataagatacc gcataaattc aaatggaaaa atttcaattt cgcgatcttc
      241 aatacggtgc gtttctcctt attgattctg tttatactca cgttttttgc agtgaaagat
      301 catgttgcct atctggctga aaatgtgggc gcagacacct ccagcggaat ctggaccgct
      361 ttttcagctt atactgttta tcaacctata aatttctttg agctcctgca ctggaatatc
      421 gatacgattt tcctgatcat gatgccaatt ttggtcattg ccagcctgat aatctaccgt
      481 cccttctgct acctgatctg cccgattggt gcgctcacat ggctgtgtga gaagatcgct
      541 cccggacggg tgcgggtaga tcacaataag tgcacgctgt gcggaaactg cgaggaacag
      601 gccccatgtc ctaccatcaa gcccatgctg gaggaaaata gcagggttct accggactgt
      661 acatcctgcg gagactgcct gggatcctgc gaagaagatg caatcaaatt cagttttaag
      721 cgctaattta aaaaaatcag gttatcaaat aacctgaatt accttgcata ttgttagaaa
      781 tctgttagtt tatatttata acggataccg ggacctttac ctgcgagtgt gcgcagagca
      841 gcaagaataa tatacatttc gcaattttac tgcaatcgga ggaaaattat gtttaagcta
      901 aaccggagag aaaaaccgtc cggcatgatt gctttttggg cgtttttctg gctgatgttc
      961 ctcgctctca gcctgtgcgc tgaggatgtg gggttctggc ccccagggca gaaatcttcg
     1021 gattattcca gctccccaat ttggacctac gggggagatc ttcctgtcgg gcatatcgag
     1081 gttgctgacc tcaacgggga caatcttaag gatatcatag ctgcagaata taaccccgac
     1141 aactatagcg actcagcgct ggtctatgcc ctggatggtt ttaccggaga tacgctgtgg
     1201 acattctggt tcgatgatgg cgtcagagtg cttattagtg gcgatatcaa tctggacaat
     1261 gtgccggaca ttatcggagg tgcagccctg ggtatcgata tagccgtgga tggccatgtt
     1321 cgcgctattg acggcgctac cggcgatgag atctggagtt ttgcaacagg aggttcaatc
     1381 acggaccttg ccatcggaaa cttcaataac agcggtaatc tggatgtggc tgtggtcggc
     1441 tatgacgact atgtctatgc tgtcgacggc tcgaccggag atgatatctg gagcagatat
     1501 ctgacatcgg tttatatcaa tgccgtggcg gttggagacg tcaacgagga cggcatagat
     1561 gatgtcggct acgcccacga gaagctgacc ggttttgata attatatggg agtgctgaac
     1621 ggcgtggacg gttctgtgat ctgggagcaa atcaacacct atttcggaac ggatttgctg
     1681 atcgacgata tcgatgatga cggcctcctg gaagcagttt ttggtttgca tttcgatgat
     1741 gatcatggcg agatccaggt tcgtaatgcc ctgacagggg gacttgaatg gagctatgat
     1801 ttcgggccta tgaacagcac ctatggcgaa ttttacctga attcaatcga tatcgatgat
     1861 gataaggata aggatctgat agtcggaaat tatgcggccc agaagaaact ctatatattt
     1921 gatggggata ataatattcc cgctgtggtt tcacccgagg tgccgagcta tatttaccag
     1981 actgcggccg gtgatgtcga taatgatggt ttcctggata ttattgcggc ttcttctgac
     2041 agggtgcaga tcttcaattc cgccacaggt aatttaatgt ggtactttgg cgtaaacgga
     2101 aacatcaatg acgttgcctg cgccgatttt gacggcgatc tctggacaga catagccgcc
     2161 ggagggggag ccaatttcag tcccagcccg cttcagactg tctgggctct gaaaacaatt
     2221 cagaccccgc tattatggca gtacaatttc gggcgctacg gtaacgccct tacagtggtc
     2281 gatctcgatc aggacggcca tgacgatgtg gtcacggttg cctcgctgga cgacagagcc
     2341 accgccatcg acggcaagac cgggaatagc attctctgga gctggaccgg taccgaaaat
     2401 ctctacgctg tcacacacgg agattttgat aatgacggag atcaggatat cgccgtagcc
     2461 ggatatgacc agacggttac cgccttagac ggtctgagcg gagaagtgct ctggcagttt
     2521 accacaccga cagcgcagat ataccgttcc tgcctggtct cgactgactt gaatggcgat
     2581 ggggccgatg atgtgattgc aggatcggat gacagctttg tatatgcgat caatggcctg
     2641 aacgggatgg agctctggaa ttatccgcca ggcgacgccc tggaggaggt ggcgctcggt
     2701 caaatgaacg gtaccggtcc gctcgatgtg gtctgcgggt tgaccaattc cataaccggg
     2761 agccgggtgg ttgtactgga cgggagtgac ggttcatggc tctggcaata caactgcaat
     2821 tacagggtgg aatatgttga ggtcttcgat gtcaacggtg atgaggttcc cgatgtggcg
     2881 gcaggagtga cctatactga tccgacactc tatatgctcg acggagccac ccatacgcct
     2941 ctctggacta tacctgtgga tatcaatccc aacaactacg gtttggcgca tggtgatgtc
     3001 aatggcgaca aagacccgga tgttatcgtg gccgggacct cggcaactaa taaggttttt
     3061 gtatacgatg gtcccaccgg cgcgttgctc tgggacttca cgatcggcgc cccggtctta
     3121 tgtgtcttag gatatgatgt caatggggat ggtgataagg aagtgatcgc cgggggcgac
     3181 gatgataaga tatatgtcat cgatggtgac ggcacagaaa tgttcagcta ctattgtgcg
     3241 gaacgggtca aacatatcca ggttggcgat atatcgggta atggcgaccc taatatcgct
     3301 tgtgtcactt tcggctcttc cggtatcgcc tatgccttca agtcgctggt gcccgagccc
     3361 aatgtcccgc cttatacgcc cacatcgcct gatcccgagg atggtgcaat cgggatttcg
     3421 gttacagcct cgctgagctg ggcgggaggc gatcccaatg ccaatgatca ggtgcattac
     3481 gatgtctact tcggcactga tgaaccgctt ccgcttgtga gtgatgatca gcttgtgccg
     3541 tcctatgatc cacccggtga tctcgaatac ggtaccgagt atttgtggca aattattgct
     3601 tttgatgaag ccgaggaatc gaccgccggc ccaatctggt ccttcaagac cgaatacgca
     3661 gttatgtgtg gcgattgtaa cgacgatttg agcgtgaatg tttccgatgc tgtctttatc
     3721 ataaactatg tctttattgg tggaaccgct cccgatccgc tctgtgtcgg tgataccaat
     3781 gctgacggat ctgtaaatgt ttccgatgcg gtagcgataa tcaactatgt tttcgtcgga
     3841 ggggatgctc ctgatccgaa ttgttgcggg aaataggaga ttttgtggca acaaaaaagc
     3901 ctcccggaat cggatccggt gggtttgaca tatttcgaca ttaatttccg tattgaggag
     3961 aatctattca tagtgcttga ttatctggct aatttgttta gattgactga aagcaaccag
     4021 aaaagttctt acctgcgaag tacacgcagt aaaatatata aagcaattaa atctttgtgt
     4081 taacgccatt tttggaggat atta
//
LOCUS       DSEB01000263            2126 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1008839, whole genome shotgun sequence.
ACCESSION   DSEB01000263 DSEB01000000
VERSION     DSEB01000263.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1008839"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(22..1011)
                     /locus_tag="ENO07_04825"
     CDS             complement(22..1011)
                     /locus_tag="ENO07_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDS01325.1"
                     /translation="MNAVIRGTGACTPEKVLTNADFEKMVDTSDDWIVSRTGIKERRV
                     VSSGESNSDLCFKAGQNALEMANISAEDLDMIIVGTVTPDYYLPSTAALVQDKLKALN
                     SAAFDVVAACAGFLHGLSIARGFILNGNVKRVLVIGSEVLSRITDYTDRSTCVLFGDG
                     AGAVVVEAEETGDQNGSGLLSSYLRADGSKKELLWIPIGGSTNPITPHNVEARERYIM
                     MSGNEIYKLAVRAMCDAALRALDQVGINTEQISWLIPHQANIRIIEGVARRLKIPDRK
                     VYLNIEKYGNTSAASVPIALDEANRKGMLKKGDYILMVAFGGGLTWGASLVRW"
     gene            complement(1020..2024)
                     /gene="plsX"
                     /locus_tag="ENO07_04830"
     CDS             complement(1020..2024)
                     /gene="plsX"
                     /locus_tag="ENO07_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDS01326.1"
                     /translation="MGGDNGPEVVLQGAYNYLRDHVGNGVKLHIVGKKDLLSDIWNSF
                     KKTDSFPIEFVHASEIVGMDEPISRGLKKRDSSLAVALRMHKMGEVDSVVSAGNTGVC
                     MAAGIRSLGRLEGVSRPAITSSFPTLKDKSVVVLDVGANVDSPPQNLYQFAVMGSIYA
                     SYINQLNRPTVGLLSIGEEKVKGNESTLQAYKLLERSSLNFIGNIEGNDILTGKCDVV
                     VTDGFAGNIILKFGESIKTYLFTKMKRQVSSNLFSRAGAFLLAPFLRRLKNTFDPAKY
                     GGAPLLGVNGVVIISHGSSNDLAIRNAIRVAVRMVNENVNEHISGKLAGHNIAENVKS
                     "
     gene            complement(2065..>2126)
                     /locus_tag="ENO07_04835"
     CDS             complement(2065..>2126)
                     /locus_tag="ENO07_04835"
                     /inference="COORDINATES: protein motif:HMM:PF01783.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDS01327.1"
                     /translation="VCPHCGYYKGREIYNPSSE"
BASE COUNT          519 a          569 c          480 g          558 t
ORIGIN      
        1 aatcagaatt atactcctgc atcaccatct gaccaaggat gctccccagg ttaatcctcc
       61 gccgaaagca accatgagaa tataatcacc tttcttgagc atcccttttc gattggcttc
      121 atccagtgca atcgggaccg aggccgccga cgtatttcca tatttttcta tgttgagata
      181 taccttacgg tccggaatct tcagccgtct ggccactccc tcaattattc tgatattggc
      241 ctgatggggg atcagccagg agatttgctc agtatttatt ccgacctgat ccagggctct
      301 taaggcggca tcacacattg cacgaaccgc gagcttatat atctcgttgc ccgacatcat
      361 aatatatctt tcgcgtgcct cgacattgtg tggagtgatg ggatttgttg agccaccgat
      421 gggaatccaa agcagttctt tcttgctgcc gtcggccctc agatacgagg aaagcaagcc
      481 tgagccgttc tgatcaccgg tctcttcggc ctccaccacg acagctccgg ccccatctcc
      541 aaaaagcacg caggttgaac ggtcggtata atcagtgatt ctggaaagca cttcggaacc
      601 tataaccaga acccgtttga cattgccgtt taagataaag ccccgggcaa ttgaaaggcc
      661 gtgcagaaaa cctgcgcagg ccgccacaac atcaaaagcc gctgaattaa gagccttcag
      721 cttgtcctgc accagggcgg cggtggaagg gagataataa tcgggggtga ccgtgccgac
      781 tattatcatg tccagatcct ctgctgaaat gttggccatc tccagagcat tttgcccggc
      841 tttaaaacag agatcggaat tgctttctcc gctggaaact actcttctct cttttattcc
      901 agtgcgggag acaatccagt cgtctgaggt atccaccatt ttctcgaaat cggcatttgt
      961 gaggactttt tcgggcgtac aggcgccggt acccctgata acagcattca tccgaaggct
     1021 cacgatttta cattttctgc aatattgtgt ccagcaagct tcccgctgat atgctcattg
     1081 acattctcat ttaccattct taccgcgacc ctgatagcat tcctgatagc cagatcgttt
     1141 gaggaaccgt gagatataat caccacgcca tttactccca aaagcggggc tcccccgtat
     1201 ttggccggat caaaagtatt cttcaggcgc ctgaggaagg gcgccagcag aaatgctcct
     1261 gctcttgaaa acaggtttga ggagacctgt ctcttcattt tggtgaacag ataagtcttg
     1321 atagactcac cgaatttcag gatgatattt ccggcaaagc catcagtaac gacgacatca
     1381 cattttccgg tgaggatatc gttcccttct atattaccga taaaattaag cgatgatcgc
     1441 tccagcagct tataggcctg caaggtcgac tcgttgcctt taaccttctc ttcgccgatc
     1501 gataagagcc ctacggtggg tctgttcagc tggttgatat acgaggcata aatcgagccc
     1561 atgaccgcaa actgatacag gttctgcggg ggtgaatcga cattggctcc gacatccaga
     1621 accacgacgc ttttatcctt gagagtggga aaggaactgg tgatcgccgg tctgctgacg
     1681 ccctccaggc gtcccaggct gcggatgccg gcggccatac ataccccggt gtttccggca
     1741 gaaacgaccg agtccacctc gcccattttg tgcattcgca gggccactgc aagcgatgaa
     1801 tcacgctttt tcaaaccgcg cgaaatcggt tcgtccatgc cgacgatctc gcttgcatgc
     1861 acaaattcta ttggaaagct gtcggtcttt ttaaaagaat tccagatatc ggaaagtaaa
     1921 tcctttttac cgacaatgtg cagttttaca ccgtttccaa catgatccct gaggtagtta
     1981 tatgcgcctt gaagcacaac ctcagggcca ttatcgcctc ccatcgcatc taaagcaatt
     2041 ctgacagttg ttttcaaatt ctacctattc tgatgagggg ttataaatct ctcttccctt
     2101 gtaatatccg caatggggac atacat
//
LOCUS       DSEB01000264            4973 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001336, whole genome shotgun sequence.
ACCESSION   DSEB01000264 DSEB01000000
VERSION     DSEB01000264.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001336"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..235)
                     /locus_tag="ENO07_04840"
     CDS             complement(<1..235)
                     /locus_tag="ENO07_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018400822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HDS01328.1"
                     /translation="MKKNVAQKLIESHLVSGKMEPGNEIGLKIDQTLTQDATGTMVML
                     ELEAMDLDRVKTELSAQYIDHNLLQTDFKNADDH"
     gene            complement(359..811)
                     /gene="rpiB"
                     /locus_tag="ENO07_04845"
     CDS             complement(359..811)
                     /gene="rpiB"
                     /locus_tag="ENO07_04845"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019979343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HDS01329.1"
                     /translation="MNDNIKNIALASDHAGFEYKEEIKKHLKGKGYEVMDFGTDSILP
                     VDYPLFIKVAAEAVALKKCQAGIVLGGSGNGEAMAANKVKGIRCAVCWNRESARLAKE
                     HNNANMISLGQRLIPLETAIAIVDEWLQAEFEGGRHKRRIDMLEKPLP"
     gene            complement(968..1717)
                     /locus_tag="ENO07_04850"
     CDS             complement(968..1717)
                     /locus_tag="ENO07_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HDS01330.1"
                     /translation="MNLTLYVWRQKDAKDKGYMEKFDAKDISPDMSFLEMLDVVNEEL
                     ESQGKDPIAFDSDCREGICGMCSLNINGVAHGPERATAACQLHMRHFKDGDTIYIEPW
                     RARAFPVVKDLIVDRSAFDRIIEAGGYISVGTGGAPDANAIPISKDDAETAFDAAACI
                     GCGACVAACKNASAMLFVAAKVSHLVNLPQGKVEAKRRVVNMVAQMDKEGFGNCTNYY
                     ECQAACPKEISVKFIAQMNREYLRSLRYKDK"
     gene            complement(1717..3630)
                     /locus_tag="ENO07_04855"
     CDS             complement(1717..3630)
                     /locus_tag="ENO07_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HDS01331.1"
                     /translation="MALDSKVPSGPISEKWDKYKFDLRVVSPANKRKFHIIVVGTGLA
                     GGSAAASFAELGYRVTAFCYQDSARRAHSIGAQGGINASKNYQNDNDSVYRLFYDTIK
                     GGDFRSREANTYRLAQLSENIIDQCAAQGVPFAREYGGKLANRSFGGALVSRTFYARG
                     ATGQQLLLGCYQALSRQIAQGNVELHVRTEMLELVVVDGHAKGIVVRDMVTGEIRSYA
                     ADAVVLATGGYSNIFFLSTSAKGCNATAMHRAYKKGALLANPCFTQIHPTCIPVHGDY
                     QSKLTLMSESLRNDGRIWVPKKKDEDRPPNEIPEDERDYYLERKYPSYGNLAPRDISS
                     RSAKEVCDEGRGVGETGVGVYLDFSDAIERLGEDTIRSRYGNLFQMYEKITAENPYEV
                     PMRIYPAPHYTMGGLWVDYNLMSNLPGLFVIGEANFSDHGANRLGASALMQGLADGYF
                     VIPYTIGHYLAGTTPGKVRTDHEEFKKAEEDVKQRVHKLLSIDGNRTVDDFHRHLGRL
                     MWEECGMARNEEGLKKALEEIPKMRQEFWENVKVVGENEELNQCLERAGRVADYLEFA
                     EVMCIDALDRDESCGAHFREEHQTEEGEAVRDDENFAYVSAWEFTEIDKKPILHKEPL
                     EFEEVKPATRSYK"
     gene            complement(3631..4356)
                     /locus_tag="ENO07_04860"
     CDS             complement(3631..4356)
                     /locus_tag="ENO07_04860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HDS01332.1"
                     /translation="MQDVNAAPTTASMPARLLNRSIGKKLLMSLSGLVLLGYVVGHLI
                     GNLQIFVGQNALNAYAVFLRSIGAWLWIIRAFLLGMLIIHVWFAVKLYFENRSARPIR
                     YKRKVYQEATLSSRTMIWTGIGVFLFVVYHLLHFTFIVTNPQYSHLEDALGRHDVYSM
                     VILGFQNYLISAVYLVSIAIVSYHLVHAAKSMFQTWGLNDTNFEKILRPATVIFAWLI
                     LLGYIAIPIAVLAGIVKLPEGVI"
     gene            complement(4499..>4973)
                     /locus_tag="ENO07_04865"
     CDS             complement(4499..>4973)
                     /locus_tag="ENO07_04865"
                     /inference="COORDINATES: protein motif:HMM:PF00361.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HDS01333.1"
                     /translation="VSLYLVVYFVTTLGAFGIISLLADQEGEKFMLESYRGMFWRRPW
                     ISLAFSGMLLSLAGVPLTAGFIGKFFVLLAGVGTALWTLVIILVITSAIGLYYYIKII
                     ATMFSRVELEAEQKPGALPVAGSTVLAGLTILLLYFGIYPVHLIRLIRDMVLAIS"
BASE COUNT         1285 a         1354 c         1052 g         1282 t
ORIGIN      
        1 gatgatcatc agcgttttta aaatctgtct gaagaagatt gtggtcgata tactgggccg
       61 acaactccgt tttcacgcga tccagatcca ttgcctccag ctccagcatg accattgtgc
      121 cggtggcatc ctgggtaagt gtctgatcga ttttcagtcc gatctcatta cccggttcca
      181 tcttgcccga caccagatgg ctctcgatca gtttttgtgc aacattcttt ttcataactc
      241 tatccctttt ataatttcat ccgaaataga tcgaatctta ttgtattaca aatgcccctg
      301 taaatactaa aatccattta cagcgccgat gttccgttta tatcagcgcc gcccatatct
      361 aaggcaatgg cttttccagc atgtctattc tgcgcttatg ccttccgccc tcaaattccg
      421 cctgcagcca ttcatccaca atcgcgatgg cggtttcaag cggaatcagc ctttgtccaa
      481 gcgagatcat gttagcgtta ttgtgctctt tggcaagacg tgccgattcc cggttccagc
      541 acaccgcgca ccttatgccc ttcaccttat tggcggccat ggcttccccg ttaccgctcc
      601 cgcccagtac aataccggcc tgacattttt tcagggccac tgcctcggcg gcaaccttta
      661 tgaaaagagg ataatcgact ggcagaatcg aatctgtgcc gaaatccata acctcatacc
      721 cttttccttt aagatgcttc ttaatttctt ctttgtattc aaacccggca tggtccgagg
      781 cgagggctat atttttgata ttatcattca taagcattcc ccatttttgt ataagttaac
      841 atgcgcttca atccagactt tcctccgata caaataaaac aaaaaaatac cgattgagca
      901 aatatctcaa ccggcgaaac atacaatttt cagccatgga actcaatgaa agcggctatt
      961 ccttaaatca cttatcttta taccttaatg aacgcaaata ttcacggttc atttgcgcga
     1021 tgaatttcac gctgatttct ttcgggcagg cagcctggca ttcataatag tttgtgcagt
     1081 ttccgaatcc ttctttatcc atctgcgcca ccatattgac cacgcgccgt ttggcctcca
     1141 ccttaccctg tggaaggttt accagatgcg agaccttggc cgccacaaaa agcatggcag
     1201 aggcattttt gcaagccgcc acgcatgctc cgcaaccgat acatgcggcc gcatcaaatg
     1261 cggtttcggc atcatctttt gatatcggga tagcattggc atccggggct ccgccggttc
     1321 caaccgagat atatccgcca gcctctataa ttctatcgaa tgccgaccga tccacaatga
     1381 gatctttcac aaccggaaat gctcttgcac gccagggttc gatatagatc gtatcgccat
     1441 ccttgaaatg ccgcatgtgc agctgacatg ccgcagttgc ccgctctgga ccgtgagcga
     1501 cgccgttgat gttcaacgag cacatcccgc atataccctc acggcagtca gagtcaaatg
     1561 ctatcggatc cttgccctgg ctctcgagtt cctcgttgac aacgtcaagc atctcaagaa
     1621 atgacatatc cgggctgata tctttggcat caaacttctc catgtaccct ttatctttgg
     1681 catccttctg ccgccaaaca tataatgtca gattcattat ttataactcc ttgtagcagg
     1741 cttcacttct tcgaactcca gaggttcttt gtgaagaatg ggcttcttat cgatttctgt
     1801 gaactcccat gccgaaacgt aagcgaagtt ctcgtcgtcg cgtacagcct caccttcctc
     1861 tgtttgatgt tcctcgcgga aatgtgcccc gcaggattca tcccgatcga gggcatcaat
     1921 gcacatcacc tcggcaaact caagatagtc ggcaactcgg ccggcacgct caaggcactg
     1981 gttcagttct tcattttcac ccacaacctt gacattctcc cagaattcct gcctcatttt
     2041 tggaatttcc tccagcgcct tcttcaggcc ttcctcattt cgagccattc cacactcttc
     2101 ccacatcagc cgtccgagat ggcggtgaaa atcatcaacc gtacggttgc cgtcgattga
     2161 aagaagttta tgaactctct gcttgacgtc ctcctcggcc ttcttgaatt cctcatgatc
     2221 tgtcctgact ttgcccggag tagtgcccgc gagataatgc cctatggtgt aaggaattac
     2281 aaaatacccg tcggcaagtc cctgcatcag ggcgctggcc ccgagacggt tggcgccgtg
     2341 atccgagaaa ttagcctcgc ctattacgaa taatccggga agattgctca tgaggttgta
     2401 atcgacccac agccccccca tggtataatg tggcgcgggg taaattctca ttggtacttc
     2461 gtaaggattc tcggccgtaa ttttctcata catttggaaa aggttgccat agcgtgagcg
     2521 aattgtatcc tctcccagcc gctcaatggc gtctgaaaaa tcgagataaa ccccgacacc
     2581 ggtctcgccc acaccgcgtc cttcgtcgca aacttccttg gccgaacgcg aggaaatatc
     2641 tctgggagca aggttgccgt aagagggata cttcctttcc agatagtaat cgcgctcatc
     2701 ttcgggtatc tcattgggcg ggcgatcttc atccttcttc ttcggcaccc agatacgtcc
     2761 gtcattacgc aatgattctg acatcaatgt cagcttggac tgataatctc catgcaccgg
     2821 gatacaggtt ggatggattt gcgtaaaaca gggattggcc aataaggcgc cttttttgta
     2881 tgcgcgatgc atcgcagttg cattgcatcc cttggcactg gtagaaagaa aaaagatatt
     2941 actgtacccg ccggttgcaa gaacaacagc atcagccgca taggatctaa tttcccccgt
     3001 aaccatgtcg cgaacaacaa ttcccttggc gtgaccatcc accaccacaa gctcaagcat
     3061 ttcagtgcgc acatgtaatt ctacattgcc ctgcgcaatt tgacgcgata atgcttgata
     3121 acatcccaga aggagctgtt gcccggttgc gccgcgggca tagaaagtgc gggaaaccag
     3181 cgcgccgcca aaggagcggt tggccagttt gccgccatat tctcgggcaa agggcactcc
     3241 ctgggccgca cattgatcga tgatattttc cgaaagctgc gccaggcgat aggtatttgc
     3301 ctcgcggctg cggaaatcgc cgcccttaat cgtatcgtaa aacaacctgt aaacagaatc
     3361 attatcattc tggtaattct tggaggcatt tatcccgccc tgagcgccaa tactatgcgc
     3421 gcgtctggcg ctgtcctgat agcaaaaggc tgtcactctg tagcccagct cagcaaatga
     3481 ggccgcggca gaaccccccg ccagacccgt tccaaccaca ataatatgaa attttcgctt
     3541 gttagctggg ctgacaaccc tgagatcaaa tttatattta tcccattttt cagagatcgg
     3601 tcccgagggt acttttgaat ccaatgccat ttaaatcacc ccctcaggaa gtttgactat
     3661 cccggccagc actgcgatcg gaatagcgat atagccgagg agtatcagcc aggcaaaaat
     3721 cacagtggcc ggacgcagta ttttctcaaa gttagtatca ttcagtcccc acgtctgaaa
     3781 catacttttg gcggcatgta ccagatgata acttactatt gcaatcgata ccagataaac
     3841 cgcggaaatc agataattct gaaagccaag tatcaccata gagtaaacat catgccgtcc
     3901 cagcgcatcc tccagatgcg aatattgggg attggttact atgaaggtga aatgcaagag
     3961 atgatagacc acaaacagga aaaccccgat accggtccag atcattgtgc gcgatgacag
     4021 cgtcgcctcc tgatatacct tgcgtttata gcgtatcggg cgggcagacc tgttttcaaa
     4081 gtataatttt actgcgaacc agacatgaat aatcagcatc cccagaagaa aagcccttat
     4141 gatccacagc cacgctccta ttgaacgcag aaaaacagca taggcattca aagcattctg
     4201 tcccacaaaa atctgcaggt tgcctataag atgtccgaca acatagccca gtagaacaag
     4261 gccgcttaaa gacatcagga gctttttgcc gattgatcta ttgagaagac gggcaggcat
     4321 gcttgcagta gtgggagccg catttacatc ttgcatagat acccaacctt tctttattat
     4381 ttgcaattaa ataatagata atctgttgtt acgtccgcaa caagactttt ttacagcttc
     4441 gaaaatgaag attttttatt agcaagagtc caatctgcca aaactatgtt atcgcttttt
     4501 aagagattgc cagtaccata tccctgatca gccttataag atgtacggga taaataccaa
     4561 aatagagcaa taaaatcgtc aggcccgcaa gaaccgtact tcccgcaaca ggaagagctc
     4621 caggcttttg ttcagcttct aactcaaccc tggagaacat ggtcgcaata atcttgatat
     4681 aataatacag cccgattgcg ctggttataa ccagaataat taccagtgtc cagagtgcag
     4741 ttccgacacc ggccaaaagc acgaagaatt tgccgataaa tccggcggtc aacggcaccc
     4801 ccgccagcga caggagcatg ccggaaaatg ccagcgaaat ccatggccgc cgccagaaca
     4861 tgcccctgta actctccagc atgaatttct cgccctcctg gtcggccaga agagagataa
     4921 tcccaaaggc gccgagagtg gtcacaaaat agaccaccag atacaggctt acc
//
LOCUS       DSEB01000265            3492 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002943, whole genome shotgun sequence.
ACCESSION   DSEB01000265 DSEB01000000
VERSION     DSEB01000265.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002943"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(187..1023)
                     /locus_tag="ENO07_04870"
     CDS             complement(187..1023)
                     /locus_tag="ENO07_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HDS01334.1"
                     /translation="MQQVEIADILARHGEKEGGLISILESIQAKYGYLPREALEEVAE
                     KTGHALVDVYGVATFYKAFSLKPRGKHLISVCLGTACHVRGGPQIAKEVKRQLGIRPG
                     ETTPDKEFTLETVNCLGACALGPVVVIDGHYFPNVKTSMVKGIIDRARDGLDKVEVDK
                     DARVFPIEVSCARCNHSLMDTEHKLDGYPSVRVTVSFGQKHGWLELSSLYGSYNTSSK
                     YEIPENTIVNFFCPYCNAELMGGSLCSECGAPMVPMIVRGGGMVQICSRKGCKGHMLD
                     LL"
     gene            1780..>3492
                     /locus_tag="ENO07_04875"
     CDS             1780..>3492
                     /locus_tag="ENO07_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01335.1"
                     /translation="MLKKFSVILFLTLFVLSMSVAAKDSAVNPQLSKTVEIRDAKLFV
                     DYPDQMQMMGVKPYASQSIAQTFAPTRQLYSNTVSSTDGQANTALGRQIAYGGYYNAG
                     CDYVYMIYMDQFIVGSSTLRQNKQAVFDWSGGVWDFTVGGLAAISDDRSGYVNADTDP
                     KNGYSVTGYHFTNGTRTWSMGGVAGPCPSDMYNFAADSMPGPPNTGNIQTGYCGDQNA
                     QTTPYIWPHIDVDTNSAGEVVIHAASNEFGCGNEPDAAIETSTLVYYRKVGANPWEGP
                     IFMDSAYLISQLVVADKTTDDVYYCYLKPLGYYTGTQDPCHANGVGHYQLGSDVVYRK
                     SIDGGSTWGPITYVTDYLSGFATQNTDPATYDLSAMVDPNGNLHLVWFSVNRGTPDNS
                     DDTQCTLYYTGKLYHYDSGNDCISVAYDASHPTFYSGSLPTFHWTVAKPNVSWCNDKL
                     YISFMRYGANPVGDTSFEIGAGIVGDTALYQVADLLVVGSDAASGSMGKTWTEAINLS
                     QTPSDSCLVGECFHETYPSMARYSTDSLMIFYMEDKDPGIWYGRGEGHETDNPLMFMT
                     WPCFT"
BASE COUNT          868 a          893 c          824 g          907 t
ORIGIN      
        1 cgccgctggc ctggccgcag gtaccggtcg gaattaccat tatcgtgcgc ggatcctttt
       61 cactggtaat cctgtcgcgc aataatttaa attcgtcaag tgatctcagt ctttgcatag
      121 agctatcccg attatatttt tgtctaattc cagttgattt attttaaacc acatctgctt
      181 gaaacattac agcaaatcca gcatatgccc tttacagcct ttgcgtgagc agatctgcac
      241 catgcctcct ccccgcacaa tcatcggaac catgggcgcc ccgcattcac tgcacagaga
      301 tccgcccatc agctcggcat tgcaataggg acagaagaaa ttcacgatcg tattttcagg
      361 aatctcatac ttggagctgg tgttgtaact gccgtaaagg ctggaaagct ccagccagcc
      421 gtgtttctgt ccaaacgata ccgtcacgcg aaccgacggg tacccatcga gcttatgttc
      481 ggtatccatc agactgtgat tacagcgcgc acagctgacc tcgatcggga acaccctggc
      541 atctttatcg acctctacct tgtccagtcc atccctggcc cggtcgatga tacctttcac
      601 catggaggtc tttacatttg ggaaataatg gccgtcgatg acgaccaccg ggcccagggc
      661 acatgccccc aggcagttca ctgtctccag tgtaaattcc ttgtcggggg tggtttctcc
      721 cggtctgatg cccagttgcc gtttgacctc cttggcaatc tgcggacctc cgcgcacatg
      781 gcaggccgta cccagacata cggatatcag gtgttttccc ctgggcttga ggctgaaggc
      841 cttatagaaa gtcgccaccc cgtagacatc aaccagcgca tgcccggttt tttcggcgac
      901 ttcttccagt gcctcgcggg gaagatagcc gtatttggcc tggatactct cgaggatgga
      961 aatcagccca ccctccttct cgccatgccg cgcaaggata tcagcgatct caacctgttg
     1021 cataacatga cctctctaat tataagatat acagatccta ttttcaaaac tgggcgagta
     1081 acccgatatg ctccggttac cggcgggtgt cgataatccg gaacccctgg atggtctgcc
     1141 gagatggagc aacttcggac agaaaacagt agtccccggg cacaaaatct cagagtcagg
     1201 attcgccttc aggcaatgca caagcaaaaa aacccgttgt aaaaagtatc cacatgcttt
     1261 attgactgca aacacccctt gccaggcgtc aagatgtttg agtaaagtaa tattgtaaaa
     1321 attttcacaa aatccgtcaa gcagaatcgc tcaaaaatct caaaaatcga cggcttaagt
     1381 ctatgaaatc aagccaaata ggttcaattc aatcaaagcg gctaaaaata atccaaattc
     1441 agaggcaata tacattcctc cgattgcgaa ctccagacta aatagattct taatatgata
     1501 cgaatactac ttgatttttt taaaattatt attattattt aggttagctt agaggcaaca
     1561 ctgggcagtc aacagtggac gagatacatg ccgaactatt tgtttttacc cgtaagccgc
     1621 caacgagtag agagttttaa tctatatatc atttgttgtg ggaaaggagg tggatggtca
     1681 cgattgagca gtcgggggtc ctttatgaca gagggactcc agaattttat tgaataaagg
     1741 tttgtctttt cttgaagtaa tcttgaaagg agttgagaga tgctgaagaa attttcagtt
     1801 attcttttcc tcacactttt cgttctaagc atgagtgttg cggcaaagga ttcggctgtc
     1861 aacccccagc tgtcaaaaac ggtcgaaatc cgtgatgcca agctgtttgt tgattatccg
     1921 gatcagatgc agatgatggg tgttaaaccc tatgcttcgc agtctattgc tcagacattt
     1981 gcaccgacac ggcagttgta tagtaacaca gtatcgagca cggacggcca ggctaataca
     2041 gctctgggac gtcagattgc ctatggcggt tactacaatg ccggatgtga ctatgtttat
     2101 atgatctata tggatcagtt tattgtgggt tcttctactt tacgccagaa caaacaggcc
     2161 gtatttgact ggtcgggagg agtctgggac tttacagtcg gaggccttgc ggctatcagc
     2221 gatgatagat caggatatgt taatgcagat actgatccga agaacggcta ttctgtaacg
     2281 gggtatcatt ttactaacgg tacccgcacc tggtcaatgg gcggcgttgc cggaccctgt
     2341 cccagcgata tgtacaattt tgctgctgac agtatgcccg gtccccccaa tactggcaac
     2401 atccagaccg gttattgcgg tgaccaaaat gcccagacta ccccttatat ctggcctcac
     2461 atcgacgttg acacaaattc agccggcgaa gttgtgatcc atgcagcctc gaatgagttt
     2521 ggctgcggca acgaaccgga tgccgcgata gagacatcga cacttgtcta ctaccgcaag
     2581 gtaggtgcca atccgtggga aggcccgatc ttcatggatt ctgcctattt gatctcgcag
     2641 cttgttgtgg ccgacaaaac cactgacgat gtttactatt gctacctgaa gcccctgggt
     2701 tactatactg gcacccagga tccctgccat gccaatggcg ttgggcatta ccagctgggc
     2761 tccgatgttg tttacaggaa gtccattgac ggcggatcca catggggtcc gataacctat
     2821 gtaaccgact acttgagcgg ctttgcaacg caaaatactg acccggccac ctatgatctt
     2881 tcggccatgg tcgatcccaa tggcaacctg cacctggtct ggttctcggt taaccgcggc
     2941 acgccggata attccgatga tacccagtgc acgctttact acaccggtaa gctgtatcac
     3001 tacgacagcg gcaacgattg tatctcggta gcttatgatg ccagccaccc gacgttctat
     3061 agcgggtccc tcccgacttt ccactggacg gtggccaagc ctaatgtctc ctggtgtaat
     3121 gataagctgt atatttcttt catgcgttat ggcgccaatc ctgtcggcga cacttcattt
     3181 gaaatcggtg caggcatagt gggagacaca gctttatacc aggttgctga ccttctggtt
     3241 gtcggttccg atgccgcctc gggcagcatg ggtaagacct ggaccgaagc aatcaacctt
     3301 tcacagacac cgtcagacag ctgtcttgtt ggcgaatgct tccatgaaac ttatccaagc
     3361 atggcgcgtt actccaccga cagccttatg attttctata tggaagataa ggatccggga
     3421 atatggtatg gccgaggtga gggccacgag actgacaacc cgcttatgtt catgacctgg
     3481 ccctgtttca cc
//
LOCUS       DSEB01000266            2908 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004405, whole genome shotgun sequence.
ACCESSION   DSEB01000266 DSEB01000000
VERSION     DSEB01000266.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004405"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            2..484
                     /locus_tag="ENO07_04880"
     CDS             2..484
                     /locus_tag="ENO07_04880"
                     /inference="COORDINATES: protein motif:HMM:PF01594.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDS01336.1"
                     /translation="MTEINRQISSYFALKFIITVGLAIVYTAGLLLMRIDYAYIWGPL
                     AAVITLIPIIGAYAGGIPPMIVAVIQYDSAIWFLWVSLFFLVVQFIESYIISPKVFGD
                     QANLNLTTVIVSTILWGWMWGIIGIILAVPMTAAIKVICSHIEPLHPIARILEGRIKF
                     "
     gene            complement(553..1674)
                     /locus_tag="ENO07_04885"
     CDS             complement(553..1674)
                     /locus_tag="ENO07_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HDS01337.1"
                     /translation="MAKKRIAWLPGDGVGVDVMEAARIVLDKVGLDAEYIHGDIGWEF
                     WCKEGDPLPQRTIDLLKDTDCALFGAITSKPKEEAEKELVPELQGKGLVYSSPIVRLR
                     QEFDLRTNLRPCKAYPGNPLNYKDGIDLVVFRENTEDLYSGVEFFPFPEDVFEVIEKK
                     NKKISRFKKYGLENVAVSLRIISEHAARNIITDAFEYAKKFGYKTVTIVEKPNVIRET
                     SGLMVRTARKVAQNYPNIELWETNIDAMCMWLIKNPFDYGVLVTSNMFGDIVSDLCAQ
                     LVGGLGFAASGNIGDDYAVFEPTHGSAPKYAGQYKVNPMAMLISCKLMLDWLGEVDKG
                     AAIDVAIAAVIKKGEFRTYDMGGTNTSLEVAQAVADHIV"
     gene            complement(1674..2588)
                     /locus_tag="ENO07_04890"
     CDS             complement(1674..2588)
                     /locus_tag="ENO07_04890"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HDS01338.1"
                     /translation="MVKVLKYSILILLTLTVFHTTSSGQVRKDDISGFSTLLVSPSAR
                     HAAMGESVGASGRDLFTLYSNPAGILNIPYLTVGAAHNEWIADFRSEYVGLVYHPSNF
                     AIGFSINYGTVDDIERRTGPTDEPEGYFDLNDLVAGVTIARNLSEKLKLGLTSKIVYE
                     KLDVYSSTGVAFDFGMQYMLMPEFMLGASFSNLGSSMKLDDEEFDLPRILRAGGSYRI
                     KDFLISGDVVYPTDDDPHLHIGGEYSLSQIFFLRSGFQSGYDEKALAFGLGFEQKGFN
                     IDYAYVPFKSDLGDTHRISLTYSLLKED"
     gene            complement(2595..2891)
                     /locus_tag="ENO07_04895"
     CDS             complement(2595..2891)
                     /locus_tag="ENO07_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01339.1"
                     /translation="MLLGFKGGVSALAFDSTTLVSVDVRKIEDHDIKFFAGGEYVFNR
                     LFALRAGYADNRVAFGGGISKTFVYYRFKLDYAFAAPVDGEAPDHLFTIGFEFR"
BASE COUNT          749 a          781 c          665 g          713 t
ORIGIN      
        1 aatgaccgag atcaaccggc agatatcatc atattttgcg ctcaaattca taattaccgt
       61 ggggcttgcc atagtttaca cagcgggact gttgttgatg cgtatcgatt atgcctacat
      121 ctgggggccg ctggcggctg tgataaccct cataccgata ataggtgcct atgccggcgg
      181 aataccgccc atgattgtgg cagtcattca gtatgactcg gcgatatggt ttttgtgggt
      241 atccttgttc ttcctggtcg tgcaattcat cgagtcctat attatcagtc ccaaggtctt
      301 cggcgatcag gccaatttaa atcttactac cgtgattgtc tcaaccatcc tgtggggatg
      361 gatgtggggc attatcggga ttattctggc ggtaccgatg acggcggcaa ttaaggtgat
      421 ctgctcccat atcgaaccgc tccatcccat agccagaatc ctggaaggcc gaataaaatt
      481 ttaagatgcc cgcgcagtct ataaatgaaa aacgcccgca ccggacgggc gttttttttg
      541 ataatctata atttacacaa tatgatcggc gaccgcctgc gctacttcca gggaggtgtt
      601 ggtgccaccc atgtcataag tacggaattc gcccttttta attaccgcgg caatcgccac
      661 atcgatcgcc gcgcctttat cgacctcgcc cagccaatcc agcatcagct tgcatgaaat
      721 caacatagcc atcggattga ctttgtactg cccggcatac ttgggcgcgg aaccatgcgt
      781 tggctcgaag acggcgtaat catcaccgat attgcccgag gcggcgaagc cgaggccacc
      841 cacaagctgg gcgcagaggt cggaaactat gtctccgaac atatttgatg tcaccagcac
      901 gccgtaatcg aaggggttct ttatgagcca catgcacatg gcatcgatat tggtttccca
      961 cagttcaata ttcggataat tctgggccac cttgcgcgcg gtacggacca tcagaccgga
     1021 ggtctcacgg attacattgg gcttctcgac aatcgtaacg gttttatatc cgaatttttt
     1081 tgcgtactcg aaagcatcag tgataatatt tctggcggca tgctccgaaa taattctcag
     1141 cgaaaccgca acattttcga gcccgtactt tttaaatctc gaaattttct tattcttctt
     1201 ttcaatcacc tcaaaaacat cttcagggaa tggaaagaac tcgactcctg aatacaagtc
     1261 ctcggtgttt tcacggaata ccaccagatc aataccgtct ttataattca gaggattacc
     1321 cggatatgcc ttgcagggac gcaggttggt gcgcaggtcg aattcctggc gcaggcgcac
     1381 tataggtgag gaataaacca gccccttacc ctgcaattcg ggaaccagct ccttctcggc
     1441 ctcttccttg ggcttggagg tgatcgcgcc gaacagcgcg caatcggtgt ctttcaataa
     1501 atctatggtg cgttggggaa gcggatcccc ctccttgcac cagaattccc agccgatatc
     1561 gccgtggata tattcggcat ccagaccgac cttgtccagc acaatcctgg cagcctccat
     1621 aacatcaaca ccgaccccgt cgccgggcag ccaggcaatt ctcttcttag ccactaatcc
     1681 tcctttaaca gcgaatatgt aagtgaaatc ctgtgcgtat cccccaaatc cgatttaaag
     1741 gggacgtatg cgtagtctat attgaaaccc ttttgttcga aaccgagccc gaaggccaga
     1801 gccttttcat catatccgct ctggaatccg cttcgcagga agaaaatctg actgagactg
     1861 tactctccgc caatatgaag atgcggatca tcatcagtcg gataaaccac gtcacccgag
     1921 atcagaaaat ccttgatccg gtatgatccg ccggcccgca aaattcgggg caggtcgaac
     1981 tcttcatcgt cgagcttcat ggaggagccc aggttggaga agctggcgcc cagcatgaac
     2041 tcgggcataa gcatatactg cattccaaaa tcaaaggcca ctccggttga ggaataaaca
     2101 tccagctttt cgtaaacaat cttcgaggtc agtcctaact taagtttctc gcttaaattt
     2161 cgtgctatag ttacacccgc gacaagatca ttcaggtcaa aataaccctc cggctcatca
     2221 gtggggccgg tgcgtctttc gatatcatcc accgtcccat aatttataga aaaaccgata
     2281 gcgaaattcg agggatgata gaccagtccg acatactcgc ttcgaaaatc ggcaatccat
     2341 tcattgtgag cggcccccac agtaagatag gggatattga ggatacccgc cggattggaa
     2401 tagagcgtaa aaagatccct cccgctcgca cccacactct cacccatggc cgcgtgacgg
     2461 gccgagggtg ataccagcag ggtcgaaaag cctgagatat catcctttct gacctgtcct
     2521 gaggaggtag tgtgaaagac ggtcagggtt agcaaaatta aaatcgaata tttcaaaacc
     2581 ttcacgataa cctcttatct aaattcaaat ccaattgtaa acaggtgatc cggggcctcg
     2641 ccgtcaacag gtgcggcaaa ggcataatcc agtttgaatc tatagtaaac aaatgtcttc
     2701 gatatccctc ctccgaaggc gacacgatta tcagcatagc cggccctcag ggcgaacagt
     2761 ctattgaaaa catattcacc gcccgcaaag aacttgatat catggtcctc tatcttgcgc
     2821 acatccacac tcaccagagt cgtgctgtcg aatgccaggg ccgagacgcc gcccttgaat
     2881 cctaacagca aatcctcatc ctgggcca
//
LOCUS       DSEB01000267            5546 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001043, whole genome shotgun sequence.
ACCESSION   DSEB01000267 DSEB01000000
VERSION     DSEB01000267.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5546)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001043"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..335)
                     /locus_tag="ENO07_04900"
     CDS             complement(<1..335)
                     /locus_tag="ENO07_04900"
                     /inference="COORDINATES: protein motif:HMM:PF01225.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01340.1"
                     /translation="MITGKLSDLKKVTNGSLFSDKAATAEFTGLSIDTRTIADGNLFV
                     AIKGELDDGHKYLRHAFDKGAGACVVQSGYEEHLPAEYRDRCLIVNDTQKGLRQIALW
                     WKDKFNPRY"
     gene            complement(307..1887)
                     /locus_tag="ENO07_04905"
     CDS             complement(307..1887)
                     /locus_tag="ENO07_04905"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:PRK00139.1-4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDS01341.1"
                     /translation="MLSLRCRSAVSPSSSGTMSCLFVKTAKRRVTIKISDLIQILPEK
                     QVSGETEREISKIEYDSRLIEPEDMFVAMPGSRDDGHDYIPDAIRAGAVCVVCERDFE
                     NSSVCKVIIPDSRHALSLLAAKFYDFPSRKMLVAGITGTNGKTTTVYMVKSILDAGNK
                     RTGLMGTIEYLAGKYQFNAPNTTPESLHIERLLSIMQAERIRNVVMEVSSHGLKTGRV
                     NMIDFNVAGFTNLTQDHLEFHGTMEEYREAKAMLLDKVKGKDRWAVLNMDDPSYDFFL
                     GRTECSCLTYSIENTRADLHITGIEKTDRGYRFKLISPLGSEEIHLKLPGRYNLNNAL
                     CAAGVALASGVNTDTVRSGLEAMTFVPGRMERVDINRDFKVFVDFAHTPDALGHVMKS
                     AREIAGDRNLIAVFGCGGDRDKGKRPLMAKAVSEYADIIFLTSDNPRTEDPEAIINDA
                     EAGLNNSKETHLVLDRKEAITRAVLKASEGDVVVIAGKGHENYQIVGTEKIHFDDREV
                     VREALKGQNSDNREVKRS"
     gene            complement(1790..3814)
                     /locus_tag="ENO07_04910"
     CDS             complement(1790..3814)
                     /locus_tag="ENO07_04910"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00905.20,HMM:PF03717.13,HMM:PF03793.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HDS01342.1"
                     /translation="MKNNKLRLSRFMVFFIFVAGCFIVIAGRFFQVQVLQGEEYARKF
                     RNQCYLNVPVKAHRGAIYDRNGNPLAYTTETENLLVYTDSDETIEKIAREAAPILDVS
                     ESSLLKKLMARKGKQVLIARKIDPGVSTRINALKIPEIYSKTEFDRIYPYNSAVSALV
                     GYLNHEFEAKAGAEVYCDKYLKGKDGLRACMKAGSGQLYPIFSEPLIEPVEGNDVYLT
                     IDIEYQQILSEEIRTAVDEWSAVSGMGVLMEVATGKILAVYHYDPELKDPDYKCPKAR
                     AITDLFEPGSTFKTVVFGALLEEGLINLADTIYAGNGSFKFNGISIHDDKKHDIISVA
                     EAFIISSNVATGRLANILGPKMLYRYAREMGFGLPTGLGFPGEVNGRLHEPEVWSEYY
                     CAMLSIGHEVSASTLQMARMFGCIANDGRLMKPILLDRVISPTGGTQQRFYPEQERKI
                     FSESTIASLKKLCEIVVDTGTAKYAKIDGIDFAGKTGTAEKPSETGGYDKSRYIASFG
                     GYFPADDPQICGIIIIDEPKEIHYGGITAGPAFAKTAKRITDLENRKNETSGRTIYAS
                     IDALKSGALNLLDEDYEGDPDVMIKHADYKLDTKAEINPVGESEWVRLPDLRNKSARD
                     AFTILNSMGLKCSLKGAGYVIATVPERGESVVLGDHVMLVCEDSKKEGDD"
     gene            complement(3811..4203)
                     /locus_tag="ENO07_04915"
     CDS             complement(3811..4203)
                     /locus_tag="ENO07_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01343.1"
                     /translation="MKRLLPEIKIYVLVGLAILLAYAFIWQRITLQNLSAEIARMEEE
                     IRQLEKNKDYLKGEILYRSSLEQVRKKAMEELDMINSSGTDIIPYSDSLLQMVKDQNS
                     SGGDGPPAFVEKNIQNGENDIIRDRIVK"
     gene            complement(4266..5162)
                     /gene="rsmH"
                     /locus_tag="ENO07_04920"
     CDS             complement(4266..5162)
                     /gene="rsmH"
                     /locus_tag="ENO07_04920"
                     /inference="COORDINATES: protein motif:HMM:PRK00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDS01344.1"
                     /translation="MGKEVVEYLVHDPSGIYIDGTCGTGGHARLIAENLSDKGRVICC
                     DLDPRMLELAKRRNVEVGDKIKHLQCGYHEVSEQLGSEDKPIAGFLLDLGISSVQLEQ
                     GYGFAFKSDNPLDMRFDPESSLTAADIINHYPLQELKKIFKEYGELSQAGPISHAIIR
                     AREKNRIKTTYQFKIILEPFFKKEKLNKGLAQVAQALRIAVNNELENIKKGLRSLTKL
                     LHSGGRLVVISYHSLEDRIVKNYIRTYSRESGLPPDLEEVLENREFRLKPLFKKALTP
                     TSQELENNPRARSAKLRAAAKV"
     gene            complement(5182..5520)
                     /locus_tag="ENO07_04925"
     CDS             complement(5182..5520)
                     /locus_tag="ENO07_04925"
                     /inference="COORDINATES: protein motif:HMM:PF02381.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01345.1"
                     /translation="MLAKWLDGCLGLFIESEWIKKFNELQSEHSGMTREGRNFIRKLS
                     TNTYEVVPDTQGRINIPRDLIQEADLKGETLMIGSTTHIEIWNPERFEKLSNANPSLE
                     NMLDQLFAKK"
BASE COUNT         1264 a         1502 c         1255 g         1525 t
ORIGIN      
        1 atatatcgag gattaaattt gtctttccac cagagagcta tctggcgcag tcccttctgg
       61 gtgtcattta ctatgaggca gcgatccctg tactcggccg gaaggtgctc ttcatacccg
      121 gattgcacca cacaagcccc ggcgccttta tcgaaagcgt gcctgagata cttatgcccg
      181 tcatccagct cgcctttgat ggcgacgaac aggttaccgt cggctatcgt gcgggtatcg
      241 atagaaagcc ctgtaaattc tgcggttgcc gctttatctg agaacagcga accattggtg
      301 acctttttaa gatcgcttaa cttccctgtt atcactattc tgtcccttta gagcctcccg
      361 gacaacttca cggtcgtcaa aatgtatttt ctcggttcca acaatctgat aattttcatg
      421 ccccttaccg gcaatcacta ccacatcgcc ttcggaggct ttcaaaaccg cccgggtaat
      481 cgcctccttg cgatcgagca ccagatgtgt ctccttgctg ttattcaacc cggcctcggc
      541 atcatttatt atggcctcgg gatcctcggt gcggggatta tccgaggtca ggaaaattat
      601 atctgcatat tccgataccg cctttgccat caggggccgt tttcccttgt cgcggtcccc
      661 accgcagccg aagaccgcga tcagattcct gtctcccgcg atctccctgg cggacttcat
      721 gacatgaccc agggcatcgg gggtatgagc aaagtccaca aagaccttga aatcccgatt
      781 gatatcgacc ctttccatgc gtcccggcac gaacgtcatc gcctccaaac cgctcctgac
      841 cgtatcggta ttaacgcctg acgcaagagc cactccggcg gcgcagagag cattattcag
      901 gttgtacctg cccggcagtt tcagatggat ttcctcgctg ccgaggggtg aaatcagctt
      961 gaacctgtat cctctgtcag ttttctcaat acctgttata tgcagatcgg ccctggtatt
     1021 ttcgattgaa taggtcaggc aggagcattc ggtgcgcccc aggaagaaat cataactggg
     1081 atcatccatg ttcaatactg cccatctgtc cttaccctta accttgtcga gcagcatagc
     1141 tttggcctcg cgatactcct ccatggtgcc gtgaaactcc agatgatcct gggtcaggtt
     1201 ggtgaacccg gcgacattga agtcgatcat atttactctg ccggtcttga ggccgtgcga
     1261 agaaacctcc atcacgacat tgcggattct ttccgcctgc ataatcgaaa gcaggcgttc
     1321 tatatgcaac gattccggag ttgtgttggg cgcattgaac tggtattttc cggccaggta
     1381 ttcgatagtg cccatcaggc cggtgcgctt gttgcctgcg tcgagaatcg acttgaccat
     1441 gtaaaccgta gtggttttgc cattggtgcc ggtgataccc gccaccagca ttttacggga
     1501 agggaagtca tagaatttag ccgccaggag cgagagcgca tgacggctgt ccgggataat
     1561 taccttgcag accgaactat tttcgaagtc cctttcgcac actacgcata cggccccggc
     1621 ccggatggca tcaggaatgt aatcatggcc atcatccctg cttcccggca tggctacaaa
     1681 catgtcttcc ggctcaatta aacgcgaatc gtattctatt ttggaaatct ccctctccgt
     1741 ttccccggag acttgttttt ctggaaggat ctgtatcaaa tcggaaatct taatcgtcac
     1801 cctccttttt gctgtcttca caaacaagca tgacatggtc cccgaggacg acggactcac
     1861 cgcgctccgg caccgtagcg ataacatatc cggcaccttt aagggaacat ttcaggccca
     1921 tgctattgag tattgtaaaa gcatcacgag ctgatttatt tctcaaatcg ggcaaccgaa
     1981 cccattctga ttctccaacg ggattaattt cagcttttgt atccagctta taatcggcat
     2041 gtttgatcat gacatctgga tctccctcgt aatcttcatc cagcagatta agtgcgccac
     2101 ttttgagagc atcgatcgag gcataaattg tcctgcccga agtttcattt tttcgattct
     2161 ccaggtctgt tattctcttt gctgttttag caaatgcggg gccggcagta atcccgccgt
     2221 agtgaatttc tttgggctca tcgataatta taatcccgca aatctgcgga tcatcggccg
     2281 gaaaatagcc gccgaaagaa gcgatgtatc tggacttatc atatcctcct gtctcggagg
     2341 gtttttcggc ggtgccggtt tttcccgcaa aatctattcc gtcgattttt gcatacttgg
     2401 cagtaccagt atcaactaca atttcacaca atttttttaa gctggctata gtggactcgg
     2461 aaaatatttt tcgttcttgt tcggggtaga agcgctgctg ggtgccacct gtgggcgaaa
     2521 tgacacggtc cagcaaaata ggcttcataa gacggccgtc attggcaata catccaaaca
     2581 tgcgtgccat ctggagggta ctggcagaga cctcgtgccc gatagaaagc atggcgcagt
     2641 aatactcgga ccagacttcc ggctcatgca ggcggccgtt gacctctccc gggaatccca
     2701 gtccggtggg caatccgaag cccatctcgc gcgcataacg atagagcatt ttggggccca
     2761 gtatattcgc cagacgaccg gtggcaacat ttgaggagat tataaacgcc tctgccacgc
     2821 tgataatatc atgtttcttg tcgtcatgga tgctgatacc attgaattta aaactgccgt
     2881 ttccggcgta gatcgtatcc gcaaggttga tcagtccttc ctctaagagc gcgccgaata
     2941 caaccgtctt aaatgtactc ccgggctcga acagatcagt aatcgccctg gctttgggac
     3001 atttgtaatc aggatctttc agttccggat cataatggta aaccgccaga atctttccgg
     3061 tagccacttc catcaggaca cccatcccgc tgacagcgct ccactcatcc accgctgtcc
     3121 ggatctcctc cgagagaatc tgctggtatt cgatgtcaat cgtcaaatag acatcattac
     3181 cctcaacggg ctctatcaat ggctcagaga aaatcgggta aagctggccg gaaccggcct
     3241 tcatacaggc gcgcaagcca tccttgccct tgagatattt gtcacagtac acctccgcac
     3301 cggctttggc ttcgaactcg tgattcagat atccgaccag ggccgaaacc gcgctgttgt
     3361 atggatagat ccgatcgaat tccgtcttgc tgtaaatctc gggaattttc agcgcgttga
     3421 tcctggtgga gacaccggga tcgatcttcc gtgcgataag gacctgtttg ccctttctgg
     3481 ccatcagttt tttcaataat gagctctcgg aaacgtccag aataggtgcc gcttccctgg
     3541 caatcttttc aatcgtttcg tccgagtcgg tatagaccaa gagattttcg gtctcggtgg
     3601 tgtaggccag cggatttcca ttacggtcgt aaatcgcgcc tcgatgagct ttaaccggca
     3661 catttagata acattgattc cggaacttgc gtgcatactc ctctccctgc agaacctgca
     3721 cctgaaaaaa acgtccggca atcacaataa aacagccggc cacaaatata aaaaatacca
     3781 tgaagcgtga gagcctgagc ttattgttct tcatttaact atcctgtccc tgataatatc
     3841 gttttctcca ttctggatat ttttttccac gaaggccggc ggaccgtccc ccccgctgct
     3901 attctggtcc ttcaccatct ggagaagtga atcggaatat ggtatgatat cggtcccgct
     3961 ggaattaatc atgtccaact cttccatggc tttctttctc acctgctcaa gcgagctgcg
     4021 gtagaggatt tctcccttga gatagtcctt gtttttttcc agctggcgaa tctcctcctc
     4081 catgcgggcg atttcggctg acagattctg caatgtgatt ctctgccaga tgaaggcata
     4141 agccaggagt atggccaggc cgaccaagac atatattttt atttccggca agagtctttt
     4201 caaactgtcc ctcattcagt caggcgggac ggttatcccg cctgactgcc tctaacaagt
     4261 gcccttcata ctttcgccgc cgcccgcagt ttggcgctgc gagcccgtgg attattctcg
     4321 agctcctgac tggtgggagt cagagctttt ttaaaaagcg gcttcaacct gaactcccgg
     4381 ttctcgagaa cctcctctaa atcaggcggt aaaccagatt cgcggctgta tgtacgaata
     4441 tagttcttta ctattctatc ttcgagggag tgataagata tcaccactaa tctgcccccc
     4501 gaatgcagta attttgtgag agaacgaagc ccctttttta tattctcaag ttcattattg
     4561 acagcaattc tcaatgcctg cgcaacctgc gccaggccct tgtttaactt ttcttttttg
     4621 aagaatggct caagtattat cttgaactga taagtagttt ttatcctgtt cttctcccgc
     4681 gcccttatga tggcatggga tattggcccg gcctggctca actcgccgta ttccttgaaa
     4741 atctttttga gttcctgcag gggataatga tttattatat ctgccgcggt caggctgctc
     4801 tcgggatcga aacgcatgtc aagcggattg tcggacttaa acgcaaatcc gtagccctgt
     4861 tcaagctgca cagagctgat tcccagatcg agcagaaaac ctgcgatcgg cttgtcctca
     4921 gaaccaagct gctcggagac ctcgtgatat ccgcattgca ggtgcttgat cttatctcct
     4981 acttccacgt ttctcctctt cgccagctca agcatgcggg gatcgagatc acagcagata
     5041 actctcccct tatccgaaag attctcagct atgagcctgg catgaccgcc tgttccacag
     5101 gtgccatcta tgtagattcc tgacgggtcg tgaaccagat attcaacaac ctctttcccc
     5161 attacgggta aatgatactc ctcatttctt tgcaaatagc tgatccaaca tattctctaa
     5221 actagggtta gcattcgaga gtttttcgaa tctctcagga ttccagatct caatatgagt
     5281 ggtggatcca atcatcagag tctcgccctt taagtccgct tcctgaatca ggtccctcgg
     5341 tatattgatc cttccctgag tatcgggaac gacctcatac gtattggtgg atagtttacg
     5401 gatgaaattg cgtccttcgc gagtcatgcc ggaatgttcg ctctgaagct cattaaattt
     5461 cttgatccac tctgattcga tgaacaatcc caggcatcca tccagccact tcgccagcac
     5521 gtaagccttc actgcctttt tgggag
//
LOCUS       DSEB01000268            1894 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1011426, whole genome shotgun sequence.
ACCESSION   DSEB01000268 DSEB01000000
VERSION     DSEB01000268.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1011426"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..484
                     /gene="groEL"
                     /locus_tag="ENO07_04930"
     CDS             <1..484
                     /gene="groEL"
                     /locus_tag="ENO07_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525521.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; many bacteria have multiple
                     copies of the groEL gene which are active under different
                     environmental conditions; the B.japonicum protein in this
                     cluster is expressed constitutively; in Rhodobacter,
                     Corynebacterium and Rhizobium this protein is essential
                     for growth; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HDS01346.1"
                     /translation="TETEMKEKKARVEDALHATRAAVEEGIVPGGGVAYLRALNALEK
                     VNLDEEEKVGVMIVRKALEEPIRQIAFNSGIDGSIVLNKVAEQNGAFGYNALTDTYED
                     LLKAGVIDPTKVARTALENAASIAGLLLTTEALVTEKPEKKKEMPGAGMPPGGMDEWD
                     "
     gene            645..1274
                     /locus_tag="ENO07_04935"
     CDS             645..1274
                     /locus_tag="ENO07_04935"
                     /inference="COORDINATES: protein motif:HMM:PF00497.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDS01347.1"
                     /translation="MSNPKMIVNSSNLRTICILTISISIILSAAALCQQTGGEKLIIG
                     TKPTAPFAMKAEDGSWYGISIELWRTIASDLELEYVFEERDLQGLLEGLEDGSLDLVV
                     SALTINSEREEKFDFSHPFYSAGLTIASSASGGASFLNVAQGLLSSQFIRVVAILVFI
                     LLVTGFLVWAFERKRNAEQFGGGIGKGIGSGFWWSAVTMTTVGYGDKAP"
     gene            1292..1750
                     /locus_tag="ENO07_04940"
     CDS             1292..1750
                     /locus_tag="ENO07_04940"
                     /inference="COORDINATES: protein motif:HMM:PF00497.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDS01348.1"
                     /translation="MALIWMFAAIIIISSLTAAIASALTVSKLEARISGPEDLPRVEV
                     GTIASSTSEKYLQNNKINFSTYDSPGDGLKAVAQGEIDAFVYDAPILRYLINNNFKGK
                     LKTLPSTFLHQDYGIALSNGRPLREAINIVLLREIARPEWESLLDRYMGK"
BASE COUNT          486 a          437 c          502 g          469 t
ORIGIN      
        1 caccgagact gagatgaaag agaagaaagc gcgtgtcgag gacgcccttc atgccacccg
       61 tgcagcggtt gaggagggca tagtgcccgg aggaggtgtg gcctacctgc gtgctctgaa
      121 tgctctggaa aaggttaatc tcgatgaaga ggagaaagtg ggtgtaatga ttgtccgtaa
      181 agctctggag gaaccgatca ggcagatcgc gttcaactcc ggcattgacg gttcaattgt
      241 tctcaataaa gtggcagagc agaacggcgc cttcggctat aatgcgctga ccgacaccta
      301 tgaggatctt ctgaaggcgg gtgtaataga ccccaccaag gttgcccgga cagcgctcga
      361 aaatgccgct tctattgcag ggctgctgtt gaccacagaa gctctggtta ccgagaagcc
      421 cgaaaagaaa aaggaaatgc ccggcgcagg catgcccccg ggagggatgg atgaatggga
      481 ttgattccgc gtatgacact ataatcaggt aactactcga gcccggcttt ttatgccggg
      541 cttttattct gccggattcc atcgctgaga aaaatcttgt tctattaagt tgttttgagt
      601 acagtagagt acaggcccag tatcagtaaa tcggggaggc tctaatgagt aatccgaaaa
      661 tgattgtaaa ttcatcaaat ctgcgaacaa tttgtatcct gaccatatcg atttcaataa
      721 ttctttcagc tgctgcctta tgccagcaga ccggcgggga gaaactgatt ataggcacca
      781 aaccgaccgc tccattcgcc atgaaagccg aggatggttc atggtatggc atcagtatcg
      841 agctctggcg cacgattgcc tcggatctgg agcttgaata tgtgttcgag gagcgtgatt
      901 tgcagggatt gctggaggga ctcgaggacg gctccctcga tctggtggtc tcggccctca
      961 ccataaattc cgagcgcgag gaaaaattcg atttctctca tcccttctac agcgccggtc
     1021 tgacgatagc atcatctgca tcgggagggg ccagctttct caatgtcgcc caggggcttc
     1081 tttcctccca gttcatccgg gtggtagcca tactggtctt catactgctt gtcaccggtt
     1141 ttttggtgtg ggcctttgaa cgaaaacgca acgctgaaca attcggcgga ggaatcggaa
     1201 aaggtatcgg ttccggattc tggtggtcag cagttaccat gaccaccgta ggttatggag
     1261 acaaggcgcc ataactttcg gcgggcggat tgtggccctg atctggatgt ttgccgctat
     1321 cattatcata tccagtctta cggcagctat tgcctcggcc ctgacggtct ctaaactcga
     1381 ggccaggata agcggaccgg aggatttgcc ccgggtggag gtgggtacga ttgccagttc
     1441 caccagcgag aaatatcttc agaataataa gattaacttc agcacttatg atagtcccgg
     1501 agacggcctt aaagcagtgg cccaggggga gatagatgcc ttcgtttatg acgcgccaat
     1561 tctcagatat ttgattaaca ataacttcaa gggtaagctg aaaaccctgc catcgacttt
     1621 cttgcaccag gattacggca tcgctctttc aaacgggagg ccactacgag aagcgataaa
     1681 tattgtcctc ttgcgcgaaa tcgcccgacc ggaatgggaa agccttctcg atagatatat
     1741 gggaaaatga atgtgcggaa ttctgtatcg ggattatcaa taatcataag cgtcttcctt
     1801 tcaacaagtt aaaaaattcc gacaaaaagt gtagaacttt ttaatccagt tgttgttgaa
     1861 tattctacat aattgttgat ccatatacta aaga
//
LOCUS       DSEB01000269            5541 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001046, whole genome shotgun sequence.
ACCESSION   DSEB01000269 DSEB01000000
VERSION     DSEB01000269.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001046"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(176..622)
                     /locus_tag="ENO07_04945"
     CDS             complement(176..622)
                     /locus_tag="ENO07_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01349.1"
                     /translation="MAENIRKQFLKGFGARLLKLYSESNFRSYAHFADKLRETGFEVT
                     NDTVSKWMSKEPSEPRASAIVALSKVLNVSADYLLTGKEPESKDENLAKLLKYLNDIK
                     NQANDLSDPRLQQYARRFAALPDNLREAAEQLLRAMEEVAKKKLEE"
     gene            655..1023
                     /locus_tag="ENO07_04950"
     CDS             655..1023
                     /locus_tag="ENO07_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01350.1"
                     /translation="MSNRIRKSIKKTEFALDNAEYIIIYRGVADDLNNILLIEREYMN
                     WTKLINVSAGSQPTAAYVPKWWLEAKGIEPGDNRLKMTINSDGDLVISENDMTPKASD
                     SEIKRSSGNSAKQENNELRA"
     gene            1252..1461
                     /locus_tag="ENO07_04955"
     CDS             1252..1461
                     /locus_tag="ENO07_04955"
                     /inference="COORDINATES: protein motif:HMM:PF12728.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDS01351.1"
                     /translation="MDKLLTSKEVCEILGIKLNTLRKWTSIGKLPVIRLSGKGGPVRW
                     RARDIQSFIDGCYVPEDPIHGRDAY"
     gene            1458..1943
                     /locus_tag="ENO07_04960"
     CDS             1458..1943
                     /locus_tag="ENO07_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01352.1"
                     /translation="MKIQVEIPEVFKASELMPRDDPFEIYRDIIEESGGTQLNARRVW
                     ALLKKHRSRDNAIKQTRMSEILGLNGAMIRACVNALRRMGFYVCSGSKGYYVSREDIQ
                     ITIDHLESRAKSEWVTAACLKRIRDQDRLYEANRDKFQKPPRTINGNLFDAGINGEAG
                     I"
     gene            2179..2466
                     /locus_tag="ENO07_04965"
     CDS             2179..2466
                     /locus_tag="ENO07_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01353.1"
                     /translation="MRLCSKKHLEEIREEAYQQGRRDKSDQLRPIIKELIRNNDELNR
                     HLAGMIADRKIPVLDLPPYRDKKKHEITSCPTGRSGSNLTSLRDHETKHQE"
     gene            2512..3456
                     /locus_tag="ENO07_04970"
     CDS             2512..3456
                     /locus_tag="ENO07_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01354.1"
                     /translation="MSTKNYPTVISERTLDEGPVQLLKEFPEDQFNLAMPARVIQYLP
                     PGHKIVLSTIKLNVEDEKKGDFYPTPKQSGRISPSSQALIKIGNAAGINWFKTHITKE
                     RQPESYELLAVRATVWGQYIDMTGDLKIVTNSKSIDRKSLEATGLSKADIAKRIEFAV
                     ERCISGAMARAVKKEFGIPSSFLPKELGRPFVAGRLMPEWDMNNPLHAKLLMERGQHA
                     ADRLYGKGRTEEPEITESTVLRRSEEEPAGSETKPQKASPKPKSQRADLLEVKAEIKR
                     LCQQLGKKPPQPLDDMTEPNLRGFYQYLLNQEAEHGRK"
     gene            3443..>5541
                     /locus_tag="ENO07_04975"
     CDS             3443..>5541
                     /locus_tag="ENO07_04975"
                     /inference="COORDINATES: protein motif:HMM:PF00149.26"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01355.1"
                     /translation="MAENKLKLIHFADLHARRDNLDKVEKFFEFALQVAEDKEPDIVV
                     IPGDIWDGEVAVNENSPLYMVIRYIRRLAMLQDVYMIPGTPSHDRPGSLNIFKSLHTE
                     RQIFVLDRPELFSMCIDIKRKERGNLAVFPIPGVTKSGLMTWAKSRGLDLSPDRADVE
                     AAGLVRELFLGFRAEMARFATRHPEFIPLLLGHFTVYGSETPTGQVMTGGDIVISTTD
                     IREAGAVINCLGHIHKPQQIGENIFYSGSPFPHNWGEKEQKSVLYAEVNRHEILQLER
                     IPTPFPAMVDFDLIYYPEDSDDQPFRFLDKKTGGPAGFPDFNPHGCNQADTRIRLHMP
                     PEAKDLVNEDHIKKWLSWNDLHPNSVKFEKIIKPQSRQRCEVINEAITNLERLKAWAE
                     TTETDLAEDIPAKLHKLQSELVLEDDAGLTARGTIRLKSLRLRGSKGITWTDEIKIDF
                     EKFNSDMILFDGDNGAGKSTTFDNLHPYPFSFYYDGLNWNAKLSDHFRLEDSCRELVV
                     SIDGIDYMFLLVVDGVRGKAEYYAYRLDGEDWTTLNDGKKPSYKRVVLELFGSPELFQ
                     QGVYQVQGTNGLINKTNAELKDMFYDFLGFTEYQAIAEKAKESAQALQNEISLTEAKL
                     SEDRRALEFIPEFDAELEKQQKAIAEHKANKFLATSELEELEFKLKEREKKAATQESV
                     KKQIADLDQQLEELSTR"
BASE COUNT         1521 a         1336 c         1429 g         1255 t
ORIGIN      
        1 cccctaaagt gatcttcatt tgattacaaa atgactcttg tttttagatt ttttttaaga
       61 tttgcatgac atactgccaa tgacaaaaca taccctaaaa cgcagtcctt ccgtgtccgc
      121 acattggact tgttcgacat aatgatcccc gatcgtggtt ctatggttgg gtcatctatt
      181 cttccagctt tttcttggct acctcctcca tagccctaag aagctgctcc gctgcttccc
      241 tcagattatc aggaagagcc gcaaatctcc tggcgtactg ctgtaaccgg ggatcggata
      301 aatcgtttgc ttggtttttt atatcattta aatacttcaa taacttcgct aaattctcat
      361 ctttggactc tggttctttt ccagtcaata gataatctgc tgagacattc aacactttgg
      421 ataatgccac gatagccgat gctcttggtt cgctgggctc cttactcatc catttggaaa
      481 cagtatcatt cgttacttca aagccggtct ctcgcaattt atctgcaaag tgagcataag
      541 atcgaaaatt tgactctgaa tagagtttta aaagacgggc tccgaatccc tttagaaact
      601 gctttctaat attttctgcc atttgcataa tatatttgac ctattaactt gttgttgtca
      661 aatagaataa gaaaatccat aaaaaaaacg gaatttgccc ttgacaatgc cgaatatatt
      721 attatatatc ggggtgtagc tgatgatttg aataatatat tattgattga aagagaatat
      781 atgaattgga caaaattaat aaatgtctcg gccggaagcc agcctacggc tgcgtatgtg
      841 ccaaaatggt ggcttgaagc aaaaggaatc gagccaggag ataataggtt aaaaatgact
      901 attaactcgg atggcgatct cgtcataagc gagaatgaca tgacaccgaa ggcctctgac
      961 agcgagatca agcgatcatc aggtaatagc gctaagcagg agaataacga acttagagca
     1021 tgatggaatt tacgcataaa agaactccgg ataatttatc ggcgggaaat gcgacccagc
     1081 ccttctaagc cggacctgga tttaagaaca tgaacaagca tacccaatcc ttacacataa
     1141 gaagtgattt cttgaggggg gaaggttgag gaagcggagt ccggagaatg gcctctccgg
     1201 gctccttctt aaaaatatat caagactcct atttgtcaag gaatatggta gatggataag
     1261 ctcttaacat caaaagaagt ctgcgagatt ctgggcatca aattgaatac ccttcgtaag
     1321 tggacctcga tcggaaagct gccggtgatt cggctctccg gcaagggtgg accggtcagg
     1381 tggcgcgccc gggacattca gtcattcata gatggatgct atgtcccgga ggatccgatt
     1441 cacgggagag atgcatattg aagattcagg tcgaaatacc ggaagtcttc aaggcctcgg
     1501 agcttatgcc ccgggatgac cctttcgaaa tttaccggga catcattgaa gaatccggcg
     1561 gtactcagtt gaatgcccgc cgggtctggg ccctgctgaa gaagcaccgc agcagagaca
     1621 atgcaatcaa gcagaccagg atgtcggaga tcctcgggct gaacggggcc atgatcagag
     1681 cctgcgtgaa cgccctgaga aggatgggct tttatgtctg ctccggatcc aagggttatt
     1741 acgtcagcag ggaggacatc cagatcacga tcgatcacct ggaatcgagg gccaagagcg
     1801 agtgggtaac ggcggcctgc cttaaacgca taagagatca ggatcggctt tatgaagcca
     1861 atcgagataa atttcaaaaa cctccccgga ctataaacgg caatctgttt gacgcgggga
     1921 taaatgggga ggccggaata tgaatcagca gttcaccccc tcctttgttg taggaagagg
     1981 ccccggatgg gtgtcaacat actacctgat actccggaat ctaatttgtc ggggcctctt
     2041 tttttggttg cctccctcgc cggattgtgg attgaaacag aataaaccct atgtgatgga
     2101 aaagaggccc cgaattgcaa tgcgtctgtc tgatcagcag gtgtgccggc ggggcctcgt
     2161 gatttagaaa ggatcgatat gagactttgc agcaaaaagc atcttgaaga gattagggaa
     2221 gaagcgtatc agcagggacg cagagataaa agcgatcagc ttcggccaat tattaaagag
     2281 ctcatcagga ataatgacga attaaacagg catctggccg gaatgattgc agacagaaaa
     2341 atcccggtct tggatcttcc accctaccgg gacaaaaaga aacatgagat cacaagctgc
     2401 ccgaccgggc ggtcgggcag caatctaaca tcgctgagag accacgagac aaaacatcaa
     2461 gagtgaaatg tgatctacga tgcactttca gattaattaa ggagaactcg gatgtcaacc
     2521 aaaaattatc caaccgtaat cagtgagcga actctggacg aggggccggt tcaattactg
     2581 aaagaattcc cggaggacca gttcaatctg gccatgcccg cccgggtgat tcaatacctt
     2641 ccaccagggc acaagatcgt cttaagtacg atcaagctca acgtggagga tgagaaaaag
     2701 ggcgactttt atcctacccc taaacagagc ggccgaatct ctcccagctc ccaggcttta
     2761 atcaagatcg gtaacgccgc cggaatcaac tggtttaaga cgcacatcac caaagagcgc
     2821 cagcccgaga gctatgagct tctggcggtc agggccactg tctgggggca gtacatagac
     2881 atgaccggcg acctgaagat cgtcaccaac agtaaatcca tcgaccgcaa aagcctggag
     2941 gccacaggcc tttccaaggc ggatattgcc aaacggattg aatttgcagt ggagcgctgt
     3001 atctccggcg cgatggcccg ggcggtcaag aaagagttcg gcattcccag ctcctttttg
     3061 ccgaaggagc tgggccggcc ttttgtggcc ggaaggctga tgccggaatg ggatatgaac
     3121 aaccccctgc acgccaaact gttgatggag cggggccagc atgcggccga ccgcctgtat
     3181 ggcaaagggc gcacggagga gccggaaata actgagagca cggtcttgag aaggtcagag
     3241 gaggagcctg ccgggtccga gacaaagccc caaaaggcct ctcccaaacc caaatcacag
     3301 agagccgatc ttctcgaggt caaggccgaa atcaaaaggc tctgccaaca gctcggcaag
     3361 aaacctcctc agcccctgga cgatatgacc gaaccgaacc tgcggggctt ctaccagtac
     3421 cttctcaacc aggaggccga gcatggcaga aaataaattg aagctgatcc actttgccga
     3481 tctgcatgcc cgccgcgata atctggataa ggtcgaaaag ttctttgagt ttgccctgca
     3541 ggtcgcagaa gataaggagc cggacattgt agttattccc ggcgatatct gggacgggga
     3601 agttgcggtt aatgaaaaca gccccttgta tatggtgatc agatatattc ggcgcctggc
     3661 aatgctccag gacgtttaca tgatacctgg cacaccttcc catgatcggc cgggttcatt
     3721 gaatatcttc aagagcctgc ataccgagag gcagatattt gttttggaca ggccggagct
     3781 tttttcgatg tgcatcgata taaaaaggaa agagcgggga aatcttgcag tatttcctat
     3841 tcctggagtg accaagtccg ggcttatgac ctgggcgaag tccagaggtc ttgatctttc
     3901 tccggatcgg gctgatgttg aagcggccgg tttggtgcga gaactgtttc tgggattccg
     3961 agccgaaatg gcacggttcg cgactcgcca tcccgaattc atccctttac ttctcggcca
     4021 ctttactgtc tatggcagtg aaacaccgac cggccaggtt atgactggtg gtgatattgt
     4081 tatatctacc accgacatcc gagaggccgg ggcagtcata aactgcctcg gtcacattca
     4141 taagccgcag cagatcgggg agaacatctt ttattcgggc agtcccttcc ctcataactg
     4201 gggtgagaag gagcagaaat ccgtgctcta tgccgaagtc aatcggcatg agattctgca
     4261 gctggagcgg attccgacac cgttcccggc catggtcgat tttgatctca tttattaccc
     4321 cgaagacagc gacgaccagc cttttagatt tctggacaaa aagaccggcg gcccggccgg
     4381 ctttccagac ttcaatcccc atggctgcaa tcaggccgac accaggatcc gcttgcacat
     4441 gcccccggaa gccaaggacc tggtcaatga agatcatatc aagaaatggc tgagctggaa
     4501 cgacctgcac cccaattcgg tcaaattcga gaagatcatc aaacctcaga gccgccagag
     4561 gtgcgaggtc atcaacgaag cgataacgaa tctggagcgc ctgaaggcct gggcagagac
     4621 gaccgaaaca gatctggcgg aagacatacc ggccaagctc cacaagctac agagcgagct
     4681 ggtgctggag gatgacgccg ggcttaccgc ccggggcacc atccgtctca aatcactcag
     4741 gctgcgcggt tccaaaggga tcacctggac tgatgagatc aagatcgact ttgaaaaatt
     4801 caacagcgac atgattctct tcgacggaga taacggagcc ggaaaatcaa ccacttttga
     4861 caacctgcat ccctatccct tcagctttta ctatgacggc ctgaactgga acgccaagct
     4921 ctccgaccac ttcaggctgg aggattcctg ccgcgagctg gtggtcagca tagacggaat
     4981 tgattatatg ttccttctgg tagtggatgg ggtccgcggc aaagctgaat attacgctta
     5041 tcgattggac ggcgaagact ggaccacctt gaatgatggc aaaaaacctt cctataagcg
     5101 ggtcgtgctg gagctcttcg gctcgcccga attatttcag caaggcgtct accaggttca
     5161 gggtacgaac ggtctgatta acaagaccaa tgctgagctg aaagacatgt tctacgactt
     5221 tttgggtttc acggagtatc aggcgattgc tgaaaaggcc aaggagtccg cccaggccct
     5281 ccagaatgaa atcagcttaa cggaagcgaa gctttcggaa gatcggcggg ccctggagtt
     5341 cataccggag tttgatgctg agcttgagaa acagcagaag gctatcgccg agcataaggc
     5401 taataagttc ctggcaacat cggaactgga ggagctcgag tttaaattga aggagcgcga
     5461 gaaaaaggcc gcaactcagg aatctgttaa aaagcagatt gccgacctcg atcagcagct
     5521 ggaggaactg tccaccagga a
//
LOCUS       DSEB01000270            2222 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1008018, whole genome shotgun sequence.
ACCESSION   DSEB01000270 DSEB01000000
VERSION     DSEB01000270.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1008018"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(154..1950)
                     /gene="ptsP"
                     /locus_tag="ENO07_04980"
     CDS             complement(154..1950)
                     /gene="ptsP"
                     /locus_tag="ENO07_04980"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDS01356.1"
                     /translation="MMNKHSAEREQLNGIGVSPGIACGKAFLHISGFPEYKEEVITEK
                     SIPLEIEKVKRAFEQTSHDMEQIKAQAAESENIDLTGVLDAQILIVADPEFKNQVIDK
                     IESLHYSAIFAFTSSVNDNIELLSRSKDPYMREMIADIETVSNHILKHLLGERHKELT
                     GFVEPAILFASYFNPGEILEMPNLNVSGFVTERGGPTSHMGLFAKSLGIPAVVAVKAN
                     LKQIPSGSKIIIDGSKGTVIINPGDEEWEEYQKISHERREARFKRFQKIHELPSATRD
                     SHHVDIMANLDLPTSLDEILAHEGVGIGLYRTEFLYLKRANFPSEDDQTKVYYDIAKK
                     FAPQNVILRTFDLGGDKLTDYFKSEFEINPALGWRAIRFSLDVAPIFEVQLRAMLKAS
                     TLKNVSIMLPLITTLDEVQKSKEILERIKNDLRQEKAPFDENIKFGIMIETPAAVFIA
                     DALAREVDFFSIGTNDLTQYTLAVDRNNKRVAKHFQALHPAVLHSIKKTVESAHKAGK
                     TVGICGEIAGDPLATKLLVGLGVDSLSMNPASIPVVKGVLPKIDFEEALKFAERVLSM
                     ASEKEITEILIKDFKESNLNNSANKKGELRNA"
     gene            complement(1940..2215)
                     /locus_tag="ENO07_04985"
     CDS             complement(1940..2215)
                     /locus_tag="ENO07_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDS01357.1"
                     /translation="MQSKEVKIVNKYGLHARPSAQLVKTAAFFKSDIYLEKDGLKVNG
                     KSIMGVMMLAAEFNSTVVISADGPDEKEAVEVLSKLLSSDFQEVYDE"
BASE COUNT          529 a          566 c          492 g          635 t
ORIGIN      
        1 ccgcagacta caatattttt aattacgccg ggatcaacgt ccggaagatc cgccgatttt
       61 cctatctccc agccttcttc gaactgcgag ggaaaatcga ctattttatc atacatcccc
      121 tctgaatcat acttgtcttt taaactcgca aagtcaggca tttctcaact ctcccttttt
      181 attggcggaa ttattaaggt tgctctcctt aaagtcctta atcaatattt ccgtaatctc
      241 cttctcgctg gccattgaaa gaaccctctc ggcgaattta agcgcctcct caaaatcaat
      301 cttcggtagt acacccttaa caacaggaat ggaggctggg ttcatggaga gtgaatccac
      361 cccaagcccg accagtaact tggtggccag aggatcgccc gcaatctcgc cgcatattcc
      421 caccgttttt ccggctttgt gggcggattc gaccgttttc ttgatagagt gaagcactgc
      481 cggatgcagc gcctggaaat gcttggccac ccgtttattg tttctatcca cagccaaagt
      541 gtactgggtt agatcgttgg tgccgataga gaagaaatcc acttccctgg ccaatgcatc
      601 agctataaaa acggcggccg gagtttcaat cataatgccg aattttatat tctcatcaaa
      661 gggcgccttt tcttgacgca gatcgttctt tattctctcc aatatttctt tggacttttg
      721 cacctcgtcc agggtcgtga taagcggcag cataatactg acattcttaa gggtggaggc
      781 tttcagcatc gccctcagct gaacctcgaa gataggcgcg acatcgaggg aaaaacggat
      841 agcccgccat cccagagcag gattgatttc aaactcggat ttgaaataat ccgtcagctt
      901 atctccgccc agatcaaagg tcctcaagat tacattctgc ggggcaaact ttttggctat
      961 gtcataatat actttagtct ggtcgtcctc agaggggaaa ttggcccttt tcagataaag
     1021 gaactcggtg cgatagaggc cgattcctac accttcatgt gccagtattt catccagcga
     1081 tgtgggcaaa tccagattgg ccataatatc cacatgatgc gagtcacgag tggcggaggg
     1141 caactcgtgt atcttctgga atcttttgaa acgcgcctcg cgcctttcat gcgagatctt
     1201 ctgatattcc tcccattcct catccccggg atttattatc acggttccct tgcttccgtc
     1261 aattatgatt ttgcttccag atgggatctg tttcaggttc gcctttactg ccaccaccgc
     1321 gggaataccc agtgactttg caaaaagccc catatgcgag gtgggaccgc ctctctcggt
     1381 cacaaaaccg ctcacattga gattgggcat ctccagtatt tcgcccggat tgaaatagga
     1441 ggcaaaaagg atcgccggct cgacaaatcc ggttaattcc ttatgccgct cgcccagtag
     1501 atgttttagg atatgattcg aaacggtctc gatatcagcg atcatctccc tcatataggg
     1561 atctttggaa cgggatagca gctcgatatt gtcatttact gatgatgtga acgcaaaaat
     1621 cgccgaataa tgcagggatt cgatcttatc aatcacctgg tttttgaatt ccggatcggc
     1681 cacgatgaga atctgggcgt ccagcacacc ggtcaaatcg atgttttcgg attcggccgc
     1741 ctgcgccttg atttgctcca tatcatggct ggtctgctca aatgcccgtt tgaccttctc
     1801 gatttccagg ggaatggatt tctctgtaat tacttcctct ttatattccg ggaatccgga
     1861 gatgtgcagg aaggcctttc cgcaggcgat tccgggggag accccgatgc cgttaagttg
     1921 ttctctttca gcagaatgct tattcatcat agacttcctg aaaatcgctg ctcaataatt
     1981 ttgagagcac ctccacagcc tctttttcat cgggcccatc ggcagagatt accacggtgg
     2041 agttaaattc cgcggcaagc atcataacgc ccataatgga tttgccgttg accttcaaac
     2101 catctttttc caggtaaatg tcggatttga agaaggcggc tgttttcaca agttgggctg
     2161 atggccgcgc atgtaagccg tatttattaa ctatttttac ttctttgctc tgcaaattgt
     2221 ta
//
LOCUS       DSEB01000271            1637 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1015730, whole genome shotgun sequence.
ACCESSION   DSEB01000271 DSEB01000000
VERSION     DSEB01000271.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1015730"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..923
                     /locus_tag="ENO07_04990"
     CDS             <1..923
                     /locus_tag="ENO07_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyl transferase"
                     /protein_id="HDS01358.1"
                     /translation="HTAPKALLHVAGKPLLWHILDSLKELDFEEITFVIGFLGDKIID
                     YVKKNYDFKSRFVEQEELLGLGHAINIAIDDDERDPVLAVLGDTIVEMNWDGIIGKDK
                     NFLGVRQVEDPRRFGVAEVEGNRILKLWEKPQDPPSNLALVGVYYIKDTQLLKDSLRE
                     IIKSNIKTKNEYQVTDAFQVMLEKGAEMYTFEIEGWYDCGKSETLLETNRHLLDVCKE
                     CAPDIEGSVVIPPVFIDKSAKIKNSIIGPYASIAAGVTIKNSIVRDSIISVDAIVVNG
                     LLTGSLIGDHAEVQGHFERLNVGDSSQIGY"
     gene            982..>1637
                     /locus_tag="ENO07_04995"
     CDS             982..>1637
                     /locus_tag="ENO07_04995"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163125.1"
                     /note="catalyzes the formation of S-adenosylmethionine
                     from methionine and ATP; methionine adenosyltransferase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDS01359.1"
                     /translation="MGDKTYLFSSESVTEGHPDKICDQISDAVLDDVLRQDINGRVAC
                     ETFVTVGLVLVGGEITTTGYVDLPTLVRQVIRDIGYTNPDFGFHYNTFALLNCIGKQS
                     PDIAQGVDVGGAGDQGLMTGYASNETDELMPMPIMLAHKLTKRMADVRKSGELSYLGP
                     DGKSQVSVEYEEGKPKRVDTVVIACQHTEEILDYVGTSITEKARQEMIDKFIRPVIPA
                     "
BASE COUNT          429 a          367 c          450 g          391 t
ORIGIN      
        1 cccataccgc ccccaaggcc ctgctgcatg tggccggtaa acctcttctc tggcatatac
       61 tcgactcgct caaagagctt gattttgagg agatcacatt tgtgatcggt ttcctgggcg
      121 acaaaataat cgactatgtt aagaagaatt atgactttaa atctcgtttt gtcgaacagg
      181 aagaactact ggggctggga catgccataa atatcgccat cgatgatgat gaacgggacc
      241 ctgtcctggc tgtactgggc gatacgattg tcgagatgaa ctgggatggg ataatcggca
      301 aggataagaa tttcctcggt gtgcggcagg ttgaggatcc acgccgtttc ggagtggccg
      361 aagtggaagg caatcgcata ttgaagctat gggaaaagcc gcaggatccc ccttcaaacc
      421 tggcccttgt cggcgtctat tatatcaagg acactcagct tttaaaagac agcctgagag
      481 agattataaa atcgaatatt aaaaccaaga atgagtatca ggttaccgat gctttccagg
      541 ttatgctgga aaaaggggcc gagatgtaca cctttgagat cgagggatgg tacgattgcg
      601 gaaaatccga aacgcttctg gaaaccaatc gtcatttgct ggatgtatgc aaggaatgcg
      661 cgcccgatat agaaggctcg gtcgtgatcc cgccggtctt catcgataag agcgcgaaaa
      721 ttaaaaattc gattatcgga ccatatgcct ccatcgcggc gggagtcacg ataaagaact
      781 cgatcgtgag agattcaatt atttcggtcg acgccatcgt tgtcaacggc ctcctgaccg
      841 gctcgctgat cggggatcat gccgaggtac aggggcattt tgagagattg aatgtggggg
      901 attcctccca gatcggttat taaaccggga ggagtctgtt aatgtggaat gcagtatttt
      961 ttcaatatag aaggagaaga tatgggggat aagacttacc tcttttcatc ggaatctgtt
     1021 accgaaggac atccggataa aatctgtgac cagatctctg atgctgtgct ggatgatgtt
     1081 ctccgtcaag atattaacgg ccgcgttgcc tgcgagactt ttgtgaccgt aggcctggtt
     1141 ttggtggggg gcgagatcac cactaccgga tatgtcgatc tgcccacact ggtgaggcag
     1201 gttatccggg acataggtta taccaatccc gattttggat ttcactataa cactttcgct
     1261 cttttaaatt gcatcggcaa gcagtcgccg gatatcgccc agggtgtgga cgtaggcggc
     1321 gccggggatc agggtctcat gacaggttac gccagcaatg agaccgacga gctcatgccc
     1381 atgccgatca tgcttgcaca taagctcacc aagcgcatgg cggatgtcag aaaatcgggc
     1441 gaattgagtt atctgggccc ggacggcaaa tcccaggtat cggtggagta tgaggagggc
     1501 aagccgaaac gtgtggatac ggtcgtcata gcctgccagc ataccgagga aatactcgat
     1561 tatgtcggaa catccataac agaaaaggcc cgccaggaaa tgatcgataa attcatcagg
     1621 ccggtgattc ccgccga
//
LOCUS       DSEB01000272            2921 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004355, whole genome shotgun sequence.
ACCESSION   DSEB01000272 DSEB01000000
VERSION     DSEB01000272.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004355"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(36..620)
                     /locus_tag="ENO07_05000"
     CDS             complement(36..620)
                     /locus_tag="ENO07_05000"
                     /inference="COORDINATES: protein motif:HMM:PF00226.29"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01360.1"
                     /translation="MAMRNFEPDYYERLHVGRGADTDEIRSAFRKMAKESHPDKSHGS
                     NDDFVKVRRAYEVLSDEALREEYNRYLDLLIGQDSFLEIRPAVRDLYDDMVGYLKQIT
                     GFRNRSEYELVLKKEYRDRDKIVKIGIPLEMVCRNCFGTGGTIFGDCPVCRGKGKHMY
                     QEDVDLFIPAGSEGGEEMKLTFSGQEVRLRLRFR"
     gene            complement(931..1242)
                     /locus_tag="ENO07_05005"
     CDS             complement(931..1242)
                     /locus_tag="ENO07_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01361.1"
                     /translation="MEKTFENVALVLDAQTFVADTDYGVIRLDKVMRPEHYNLDDGKL
                     KRKLCKLIEGRKVEVNTIDTDRIGRRIAHVTVDGMSVNEIMRREIIRLYGCDNQKVID
                     N"
     gene            complement(1507..>2921)
                     /gene="dnaK"
                     /locus_tag="ENO07_05010"
     CDS             complement(1507..>2921)
                     /gene="dnaK"
                     /locus_tag="ENO07_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDS01362.1"
                     /translation="IINEPTAASLAYGLDKKNDAKIAVYDLGGGTFDISILEIGDGVF
                     EVKSTNGDTHLGGDDFDKRIIDWLVDEFKKDQGIDLSQDPMAVQRLKEAAEKAKIELS
                     STMETTINLPFITADQSGPKHLNLTLSRSKYEQMVEDLVERTVGPCKAAIEDAKLTTA
                     DIDAVVLVGGMTRMPKVVETVKKLFGKDPHKGVNPDEVVAIGAAIQGGVLTGDVKDVL
                     LLDVTPLSLGIETLGGVFTRLIERNTTIPTKKTEIFSTAEDNQTSVEVHVLQGERPMA
                     SGNKTIGRFHLDGIPPAPRGVPQIEVTFDIDANGILNVSAKDKGTGKEQNIRIEASSG
                     LTEQDIEKMVNDAKAHESEDQKRKEEIDARNSADNIAYQTEKNIKQFEDKLDEDTKSK
                     LNAALERTKKAIKDNNVPEMKSASDDLQKIWYDASSKIYQQQTAGQQGQPGPEEQQQA
                     QGQEEGAVDADFEVVDDDKK"
BASE COUNT          654 a          862 c          590 g          815 t
ORIGIN      
        1 cacccagtat ttcataaaaa tcttttccag ccatattatc taaacctcaa tctgagtctg
       61 acttcctgtc cggagaatgt cagcttcatc tcttccccgc cctcgcttcc ggcgggaatg
      121 aaaagatcaa catcctcctg gtacatatgc ttccctttac ctctacaaac aggacaatca
      181 ccgaatatcg ttccccccgt cccgaaacaa ttcctgcaga ccatctccag aggaattccg
      241 atctttacaa tcttgtctct gtccctgtat tcctttttca gcaccagctc gtattcggaa
      301 cgatttctga atccggtaat ctgttttaaa tagccgacca tgtcatcata aagatcacgc
      361 acggccggcc tgatttccaa aaagctgtcc tgcccgatca aaagatcgag ataacggttg
      421 tactcttctc tcaaagcctc atccgataaa acctcataag cccgcctgac ctttacaaaa
      481 tcgtcattgc tgccatggga tttgtccgga tgagactcct tggccatctt cctgaaagcc
      541 gatctaatct catctgtgtc ggctccacgg ccgacatgca gtctttcata ataatccggt
      601 tcgaaattcc tcatagccaa ctcgctctcc gttttaaaga gctccctttg atgtcctcaa
      661 tctatatcaa caatttcaaa ccgcaacaga ttctattatt tgagcatgtc attctgagga
      721 gcgaagcgac gaagaatctt tttgcttcaa ccataatgat tgttccatac tgattcttca
      781 gtcatatcgt tccctcaata atcagcatga tcgtaacaca tgggtggcat gcccaaactt
      841 gtttgggcat gggtcatccc cgaaccaaac ggagtctgtc aggccttgac cgatttgacc
      901 cggccacccg aacgccgcgc acgataagaa tcaattatca atcactttct gattatcgca
      961 accataaagc cgtatgatct ctcgccgcat tatctcattt accgacatac catcgaccgt
     1021 cacatgcgca atccgtcgtc ctattctgtc tgtatcaatc gtattgactt cgaccttgcg
     1081 tccttcaatc agtttgcaca gcttccgttt aagcttaccg tcatcgagat tataatgctc
     1141 cggacgcatg actttatcga gacggatcac accataatcg gtgtccgcca caaatgtctg
     1201 cgcatctaac accagagcaa cattttcaaa agtcttttcc atctcacaac cctccatgga
     1261 tatttatgtt aactttctct cactcggaga gccctctcat ataatgtaca atcaggaatc
     1321 attaatattc ccatttctcg tcacgtgccg tcgggaactc atccctctcg gaatcagagt
     1381 gccgccgaca aatttatttg tcggcgaccc ttttatcatg cttccgccac aaaacaagtt
     1441 tgtggcgggc acccatttcc gagattattg gctggcaggt cagagacctg ccagctcatc
     1501 tatctcttac ttcttgtcgt cgtcgaccac ctcgaagtca gcatccacag ctccttcctc
     1561 ctggccctgg gcctgctgct gttcttccgg accgggctga ccctgctggc cggcagtctg
     1621 ctgctggtag atcttagagg aagcgtcata ccagatcttc tgcagatcgt cggaagccga
     1681 cttcatctca ggaacattat tatccttgat cgccttcttg gtgcgttcga gcgccgcatt
     1741 cagtttcgat ttcgtatcct cgtccagctt atcctcgaac tgcttgatgt tcttctcggt
     1801 ctggtaagcg atattatccg cactgttccg ggcatcgatc tcttccttac gcttctgatc
     1861 ctcactttca tgagccttgg cgtcgttaac catcttctcg atatcctgct cggtcagacc
     1921 tgaagaagcc tcgattctga tattctgctc ctttccggta cctttatcct tggccgaaac
     1981 attcaggata ccattggcgt cgatatcgaa ggtaacctcg atctggggca ctccccgcgg
     2041 cgccggcggt attccgtcca gatggaatcg tccgatagtc ttgttaccgg aagccatcgg
     2101 acgctcaccc tgaagcacgt gaacctcgac cgaggtctgg ttgtcttcag cggtcgagaa
     2161 tatctcggtc ttcttggtcg gaatagtcgt atttcgttcg atcagcctgg tgaatactcc
     2221 gcccagggtc tcgattccga gtgagagcgg ggtaacatcc aggagcagaa cgtccttgac
     2281 atcgcccgtc aggactccac cctgaatcgc cgcgccaata gcaactacct catccggatt
     2341 tacaccctta tgcggatcct tgccgaatag cttcttgaca gtttcgacaa ctttcggcat
     2401 acgggtcata ccgcccacca gcaccacagc atcaatatct gctgtagtca gcttggcgtc
     2461 ttcaattgcc gccttgcatg gacccacggt acgttctacc aggtcttcca ccatctgctc
     2521 gtacttggag cggctcaagg tcaggttcag atgcttggga ccgctttgat cagccgtaat
     2581 gaatggaaga tttatggtcg tctccatggt ggatgacaac tcgattttgg ccttctcggc
     2641 agcctctttc agcctctgca ccgccatcgg atcctgtgaa agatcaatac cctggtcctt
     2701 cttgaactca tccacgagcc agtcgataat cctcttatcg aaatcgtcac cgcccagatg
     2761 tgtatcgccg ttggtcgact tcacttcaaa cacaccatct ccgatttcca ggatggagat
     2821 atcgaaggtt ccgccgccca ggtcgtatac cgctatcttg gcgtcattct ttttatccag
     2881 gccgtaggcc agcgatgccg ccgtgggctc gttgataata c
//
LOCUS       DSEB01000273            2146 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1008663, whole genome shotgun sequence.
ACCESSION   DSEB01000273 DSEB01000000
VERSION     DSEB01000273.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1008663"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..644
                     /locus_tag="ENO07_05015"
     CDS             <1..644
                     /locus_tag="ENO07_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HDS01363.1"
                     /translation="VDIYLDNGDHNAKAVFAEISFDPKKLAYHATSFNTQLTETDRPV
                     FVKPLQDRDKVSISAAVLGRDQVFEGSGLIATLQFNLLTSGEAELVLSDADIRDNQNN
                     NLLGEGFISEPIASAAALPDRYELGQNHPNPFNPETEISYSLPEDTWVTIKVYNISGQ
                     LVKTLVDDVKPAGRHTVSWNGTNQSNSRVATGVYFYRMETERFQKTAKMILVK"
     gene            complement(703..>2146)
                     /locus_tag="ENO07_05020"
     CDS             complement(703..>2146)
                     /locus_tag="ENO07_05020"
                     /inference="COORDINATES: protein motif:HMM:PF00144.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HDS01364.1"
                     /translation="LLSMLAGPGLARVSGAEIPATPPGEILKLLLQSFNSGDEEAWKT
                     FIANNWKESNDPQSAERRLGFFRQVYDDTEGLQLQKITDSDSLIISALLHARKPEAGV
                     EWVDLTLFVDSTDTDKLLMISARPGEDPRYEVPEGKLSHVEMADFLDAYLKDLVARDQ
                     FSGTVLIALDGEPFYTRFYGQACKRYNAPNKLDTKFNLGSMNKMFTGAAIMQLVERGK
                     ISLDDKVGNYLPDIPRKEIAEKVTIHHLLTHTSGMQDYWEEMFDTSYWELKTVDGLAS
                     LIFEDTLLFEPGSDFHYSNSGPIVLGMIIEKVSGMSYYDFIAENIYKPAGMINSGCFE
                     VDTPVPNLAIGYTHMNYDGSMLPEGLWHNNLFMHAVKGGPAGGGYSTVEDLLAFDQAL
                     RSGKIMSKESFELMSIGKTSRRPDVSYGYLIQDQNVNSHRIVGHGGGAPGINAMLDMY
                     MDSGYTVAVMANYDGAASRVAEKIRELIAK"
BASE COUNT          537 a          572 c          488 g          549 t
ORIGIN      
        1 atgttgatat atacctcgat aacggtgatc ataatgcaaa agctgttttc gcggaaatca
       61 gctttgatcc gaagaagctg gcatatcatg ccacgtcctt caacacgcag ttgaccgaaa
      121 ccgaccgccc ggtctttgtc aagccgctgc aggatagaga taaagtctcg atttccgccg
      181 cagtgctggg acgagatcag gtctttgagg gttccggcct tattgctaca ttacagttca
      241 atctgctgac ctccggggaa gccgaactgg tattgagcga tgcggatatc cgcgataacc
      301 agaataataa tctgctgggt gagggcttca tttcagagcc gatagcatca gcggcagcgc
      361 tgccggatcg atatgaattg ggccagaatc atcccaatcc tttcaatccc gaaaccgaaa
      421 tatcatacag tctgccggaa gatacatggg ttacgatcaa ggtctacaac atttcaggcc
      481 agctcgtgaa aaccctggtt gacgatgtca agccggccgg caggcacacg gtatcctgga
      541 acggcaccaa ccaaagcaat agtagagttg cgaccggtgt ttatttctat cgtatggaga
      601 cagagagatt ccaaaaaacc gcgaagatga ttctggttaa gtaattcttt caacctttaa
      661 cccgggaggg gggatgccct ctcccggttt ttttattgga ttctatttag ctatcaactc
      721 gcggattttt tccgctacgc ggctggccgc gccgtcataa tttgccatca ccgctaccgt
      781 gtagcctgaa tccatataca tatccagcat ggcattgatt cccggcgcac cgccgccatg
      841 ccccacaatt ctatggctat ttacattctg atcctgaatt aaatagccat aacttacatc
      901 gggcctgcgg ctggtcttgc ctattgacat tagctcgaat gattccttgc tcatgatctt
      961 gccgctgcgc aaggcctgat caaaagccag cagatcttcc accgtggaat agccgccccc
     1021 ggcggggccg ccctttacag catgcatgaa caaattatta tgccataacc cttcaggaag
     1081 catgctcccg tcgtaattca tgtgagtgta tccgatcgcc agattcggga caggggtatc
     1141 cacctcaaag catccgctgt ttatcatacc cgccggtttg taaatatttt cagcgataaa
     1201 atcataataa ctcatgccgc ttactttttc gataatcatc cccaggacaa tcggcccgga
     1261 attgctgtag tgaaaatcgc tgccgggttc aaacaggagc gtatcctcaa agatcaggga
     1321 agccagccca tccacagtct ttaattccca ataagatgta tcgaacatct cctcccagta
     1381 atcctgcatc cctgaggtgt gggtcaggag gtgatggatc gttactttct ccgcaatttc
     1441 tttccgggga atgtcgggca ggtaatttcc gaccttgtca tcaagtgata tcttaccccg
     1501 ctcaaccagc tgcattatgg ctgcgcctgt aaacatcttg ttcatcgaac cgaggttgaa
     1561 ctttgtgtcc agcttgttgg gagcattata acgcttacag gcctgaccgt aaaacctggt
     1621 atagaacggc tcaccatcta atgcaatcaa taccgttccg gagaactgat cccttgccac
     1681 caggtcctta agataagcat ccagaaaatc tgccatttca acatgagaaa gcttcccttc
     1741 cggcacttca tacctggggt cctctcccgg gcgtgctgat atcatcaata acttatcagt
     1801 atcggtagaa tcgacaaaca gagtcagatc gacccactcc actccggcct caggctttct
     1861 ggcatgaagc agcgccgaga taataaggct gtctgaatca gttatctttt gcaattgaag
     1921 gccttcggtg tcatcataaa cctgtcgaaa gaatccgagc ctcctttccg cagattgtgg
     1981 gtcattagat tctttccagt tattggctat gaaagttttc caggcctcct catccccgga
     2041 attaaatgac tgcaagagta atttcagaat ttcgccgggt ggggttgctg gaatttctgc
     2101 cccggagacc cgggccagcc ccggccccgc gagcatgctc aacaac
//
LOCUS       DSEB01000274            3459 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003013, whole genome shotgun sequence.
ACCESSION   DSEB01000274 DSEB01000000
VERSION     DSEB01000274.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003013"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(54..929)
                     /locus_tag="ENO07_05025"
     CDS             complement(54..929)
                     /locus_tag="ENO07_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDS01365.1"
                     /translation="MSPQKLRQPLIGAHMSIAGGVFNAIAHGVEMDCATIQLFTKSNN
                     QWKAKILTDEEVEQYFKDRVKAGINPVVAHTSYLINIASPKEDIYIKSVESLIEEIER
                     CHVLGIDDLVLHPGSYLDTTEEIGIQKIVDTLNSIFEKTHKLKTRIALETTAGQGTNL
                     GYKFEQLARMIDGIENKTRVSVCMDTCHIFAAGYDIRDKKSYEKTIRQFDDIIGLSYL
                     KAMHLNDSKMELGRKRDRHEHLGKGFIGADAFRFIMQDKRLKNIPKLLETPKERDGIK
                     MDKVNLDLLRKFHIS"
     gene            complement(929..1840)
                     /locus_tag="ENO07_05030"
     CDS             complement(929..1840)
                     /locus_tag="ENO07_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HDS01366.1"
                     /translation="MPADTKSYRRYLKPEVVSRLKTMELRARMVVEGFMTGLHKSPYH
                     GFSVEFAEHRQYMPGDNIRYIDWKVYGKSDRFYVKEYEEETNLKAYLLLDTSASMGYT
                     SDKKYLQKLEYCSYLAAAFTFLMLKQRDSVGLVGFDERIHTYIPPRSSASHLHILLQH
                     LDKQKASDKTDIAETLHEMAERIKRRGLIIILSDLLDDPQKILAGLKHFRHRKHEVIV
                     LQTLDPAEREFSFPREAIFEDMETGEEITTLPWQIKKDYQNTFNAVFDEFARECRMSN
                     IDYHRIDTSQTYDYSLFAYLNKRSKLF"
     gene            complement(1850..2863)
                     /locus_tag="ENO07_05035"
     CDS             complement(1850..2863)
                     /locus_tag="ENO07_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDS01367.1"
                     /translation="MKGDCVPDNKDIETMEKLKDARDKIKSEVGKIIVGQDKVIEELL
                     IAILSNGHVLLIGVPGLAKTLLVSTLARVLDLKFSRIQFTPDLMPSDITGTEILEEDR
                     ATGGREFKFVRGPVFANIVLADEINRTPPKTQAALLQAMQEHEVTAGGQTFTLQEPFF
                     VLATQNPIEQEGTYPLPEAQLDRFMFNILIDYPSYNEENEIVRTTTALLEQNLEVVLR
                     GEDIADLQRLVRRVPVSDHVIDYAVRLVRATRYSSDGNSLDYIKDWVSWGAGPRASQY
                     LILGAKTRAILDSRPTPSVEDVREVAKPVMRHRIVTSFNAEADGVTAEHIVGRLIEDV
                     KEK"
     gene            complement(3143..3418)
                     /locus_tag="ENO07_05040"
     CDS             complement(3143..3418)
                     /locus_tag="ENO07_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01368.1"
                     /translation="MLFDEKEFSSTLSVSTSAFREKIEQLEELADNLMYTRMAESTTM
                     HFQIARVRGAHFESQEVKVPFTEETLKALKAETDRFRRKIIPTVSKI"
BASE COUNT          773 a          886 c          838 g          962 t
ORIGIN      
        1 caataaaccc gattttcata atttatttcc tgatggttga ttagcttctt aacttacgag
       61 atatggaatt ttctcagaag gtcaagatta actttatcca ttttaatccc atcccgctcc
      121 ttgggagttt ccaatagctt gggaatgttc tttaagcgct tgtcctgcat gataaacctg
      181 aatgcgtccg cgccgataaa gcccttgcca agatgttcat gacggtctcg ctttctgccc
      241 agctccattt tggagtcatt caggtgcatg gctttcaaat atgacagccc gataatatca
      301 tcaaactgcc tgatcgtctt ctcgtagctt ttcttatccc ggatatcata acccgcggca
      361 aatatgtggc aggtatccat acagaccgag acccgtgtct tgttttcgat accgtcaatc
      421 atccgggcca gctgttcgaa tttatagccc aggttggttc cctgcccggc tgtggtttcc
      481 agcgctatcc tggtttttag cttatgcgtt ttttcgaata tgctgtttaa cgtatctacg
      541 atcttttgaa taccgatttc ctcggtggta tccagatatg atcccggatg cagaaccaga
      601 tcatctatac cgagcacatg gcagcgctcg atctcttcta tcaatgattc caccgacttg
      661 atgtatatat cctctttggg ggatgcaata ttaatcagat atgacgtgtg cgcaactact
      721 ggattgatcc cggcctttac tctatccttg aaatactgtt caacctcttc atcggtcagg
      781 atcttggctt tccattgatt attggatttg gtgaagagct ggatcgtggc gcaatccatt
      841 tcgaccccat gtgcaatggc gttgaacacc ccgccggcga tggacatatg tgcgccgatt
      901 aagggctgcc tgagtttctg aggagacatc agaagagctt actcctttta ttcagatagg
      961 cgaaaaggga gtagtcgtaa gtttgtgaag tgtcgattcg atgataatcg atattggaca
     1021 tacggcattc ccgcgcgaat tcgtcgaaga ccgcgttaaa agtgttctgg tagtcctttt
     1081 tgatctgcca gggcagggtt gtgatctcct cacccgtttc catatcttcg aaaatagcct
     1141 ctcgcggaaa ggagaattca cgttcggcgg gatcgagtgt ttgaagcacg atcacttcat
     1201 gctttctgtg cctgaaatgt ttcagtccag ccagaatttt ctgcggatcg tcgagaagat
     1261 ccgaaagaat tatgatcaat ccgcggcgtt tgattcgctc ggccatttcg tgcaaggtct
     1321 cagcgatatc tgtcttatcc gatgctttct gcttatccag atgctgcagc agtatgtgca
     1381 gatgcgaagc cgaggaacgc gggggtatat aggtgtgaat cctttcgtcg aatccaacca
     1441 gtcctaccga atcgcgctgc tttaacatca aaaatgtaaa cgctgccgcc agatatgagc
     1501 agtattccag cttctgcaga tatttcttat ccgaggtata gcccatcgaa gccgaggtgt
     1561 ccagtagaag atatgccttc aggtttgttt cttcctcgta ttccttgaca taaaagcggt
     1621 cggatttgcc gtaaaccttc caatcgatat aacgtatatt atcgcccggc atatactgcc
     1681 ggtgctcggc gaattcgacc gagaagccat gataggggga cttgtgcaat ccggtcatga
     1741 agccctcgac caccatgcgc gcccggagtt ccatggtctt cagccgcgag actacctccg
     1801 gcttcaggta tcttctgtaa ctttttgtat ctgcaggcat tttatacggc tacttttcct
     1861 tcacgtcctc gatcagccgt ccgacaatat gctcggccgt aacgccatcg gcctcggcat
     1921 tgaaggaggt cacgatgcga tggcgcatga ccggtttggc gacctcgcgc acatcctcaa
     1981 ccgagggtgt gggacggcta tccaggatgg cacgggtttt ggcgcccagg atcaggtact
     2041 gcgaggccct cggtccggcg ccccagctaa cccagtcctt gatataatcc aggctgtttc
     2101 catccgatga gtatctggtc gcgcgcacca gacgcacggc gtaatctatg acatgatccg
     2161 agaccggcac ccgtctcaca agcctctgaa ggtccgcgat atcctcaccg cgcagtacca
     2221 cctctaaatt ctgctccaga agtgcggtgg tggtgcgcac gatttcgttc tcttcattgt
     2281 agctgggata atcgatcagg atattgaaca tgaacctgtc caactgggcc tcgggcaggg
     2341 gataggtgcc ctcctgctcg atcggattct gcgtggccag cacgaaaaac ggctcctgca
     2401 gcgtgaatgt ctggccgcct gccgtaacct catgttcctg catagcctgc agaagcgccg
     2461 cctgcgtttt gggtggggtg cggttgattt cgtcggccag cacgatattg gcaaaaacag
     2521 gtcctctgac aaatttaaat tcgcgccctc ctgtggccct gtcctcttcc aatatctccg
     2581 ttccggtgat atccgagggc atcaggtcgg gggtaaactg gattcgtgag aatttcaggt
     2641 ccagtactcg cgcaagggta gagaccagaa gggttttggc cagtccggga acgccgatca
     2701 agagcacatg accgttggaa agaatggcta tcagcagttc ctcgataacc ttgtcctgcc
     2761 ccacaataat tttgccgact tcggatttga tcttatcccg cgcgtccttc agcttttcca
     2821 ttgtctcgat atctttatta tcaggcacac aatctccttt catgaaaaaa cgaaatatac
     2881 atactattac agttatttca agcaattgtt tccgggaggg gaggattggc aggaatcatc
     2941 gggtggcctg catcttcaga tgtcgggaag aatgaaatga gattagatcc caccgctaaa
     3001 gcggatgggg cacccagcct ctgctgtccg gcaggggcat tttgattcat ggcgcagggt
     3061 cacatcctgc cagggccgga caagaccccg cgcgacgaaa atatagttct tgaatctgcc
     3121 gcttgaaact tcccctctgt ttttatattt tcgaaacagt tgggatgatt tttctgcgaa
     3181 acctgtctgt ttcggctttg agagctttga gggtctcttc cgtaaacggt actttgacct
     3241 cctgtgattc gaaatgcgct cctctgaccc gggcaatctg gaaatgcatg gtggttgatt
     3301 ctgccatccg agtatacatg agattgtcgg caagttcctc cagctgctcg attttttccc
     3361 gaaaagcgct tgtactgacg gacagggttg acgaaaactc tttttcgtca aatagcactt
     3421 tctcccactc tgcgaaaatc ttctcgcccc atgtacgag
//
LOCUS       DSEB01000275            2147 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1008653, whole genome shotgun sequence.
ACCESSION   DSEB01000275 DSEB01000000
VERSION     DSEB01000275.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1008653"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..718)
                     /locus_tag="ENO07_05045"
     CDS             complement(<1..718)
                     /locus_tag="ENO07_05045"
                     /inference="COORDINATES: protein motif:HMM:PF14532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDS01369.1"
                     /translation="MERKLMERAKILVVDDEESMRDFMSIMLSKEGYEVLQADSGPAA
                     LGQIKKSRPDVIITDIMMPEMSGLELMTKAQEMHEDLSFIVMTAFASVDSAIEAMKKG
                     AYDYITKPFKVDEIKLTIKKVLEKEQIARENKELKKELSRNHSLDSFIGNDPKIIKMK
                     EMIQRLAKTDSNALILGESGTGKDLVANAIHYHSNRVGKPFLTLNCAAIPETLLESEL
                     FGYKKGAFTGALKDKEGVFKA"
     gene            927..2045
                     /locus_tag="ENO07_05050"
     CDS             927..2045
                     /locus_tag="ENO07_05050"
                     /inference="COORDINATES: protein motif:HMM:PF01208.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01370.1"
                     /translation="MIEFFVAFCKLNDYHMWNKSGMIVIYVNNGTCSPNRIGDKTLTH
                     RERIEALLSGEKPDRMPVSFWRHFYDSESTAEQLKTSMVRFQEEFDWDLMKINPRASY
                     HMEDWGNAYEYTGNPEDKPKRLEYAVHKSKDWEKINRLDPSKAEVLADHLKAIRLIRK
                     SVGDDLPVVMTVFNPISIAGDLVKDDPFLVDQIRRDPDPLQRALERITETFIDFSKAA
                     VAAGADGIFFATTQWASTSRITEDEYDKFGRKYDFMLLDEVMPHTKINVLHVCDRCNM
                     LPLFKDYPIKIVNWDMNDETNPDAAKGAEILKDKVILGGVERKGLLQKGTHAELEAVV
                     EKYKAFAQKHPFMLGPDCSIPVTTPDENLRVIKAKIEH"
BASE COUNT          559 a          504 c          532 g          552 t
ORIGIN      
        1 cagccttgaa cacgccctct ttgtccttaa gtgcaccggt aaaagcgccc tttttgtatc
       61 cgaaaagctc actttccagg agtgtctccg gaatcgccgc gcagttgagg gttaaaaacg
      121 gtttgccgac acggttagag tggtaatgta tggcattggc gacaaggtcc ttgccggttc
      181 cgctttcgcc cagaatcaag gcgttgctgt cggttttggc cagcctttga atcatctcct
      241 tcatcttgat gatcttggga tcgtttccaa tgaagctgtc gagtgaatgg ttgcgggaga
      301 gctctttttt gagctccttg ttctctctgg cgatctgttc tttttccaga actttcttaa
      361 tagtcagctt tatctcatcg accttaaatg gtttggtgat ataatcatag gcgccttttt
      421 tcatggcctc gatggcggag tccacggagg cgaaagcggt catgacgata aaggagagat
      481 cctcatgcat ctcctgcgct ttggtcatca gctccagccc ggacatctcc ggcatcatga
      541 tatctgtaat tatgacatcg gggcgggact tctttatctg gcccagcgcg gccgggccgg
      601 agtcggcctg aagaacctca tacccctcct tggagagcat gatagacata aaatcacgca
      661 tcgactcttc atcatcgacc accagaattt tggctcgttc catcaacttc ctttccatcc
      721 aagccctctc tatatgatta tcggcaggtc ttgcaaaatt caacactccc ccgccggttg
      781 ttcaaaattc gcactgagtg ggcccgatca caccttaaag tactactcaa aatattatcg
      841 gtatgtcatt aattaacaac gcacatcaaa tgaaagactt cagcagagga atctgtccgc
      901 taatgtaatc gatctttgaa gaatcgatga tcgagttttt tgttgcgttt tgcaaattaa
      961 atgattacca catgtggaac aaatccggga tgattgtaat atatgtcaat aatgggactt
     1021 gttcacccaa tcggattgga gataaaacct tgactcacag ggaaagaatt gaagcgttat
     1081 taagcggcga gaagcctgac cgcatgccgg tcagtttctg gcggcatttc tacgacagcg
     1141 aatccacagc agaacagctc aagacttcga tggtcaggtt ccaggaggaa ttcgactggg
     1201 acttgatgaa gatcaatccc cgcgcctctt accatatgga ggactggggc aatgcttatg
     1261 aatacaccgg aaatcccgag gataaaccca aacggctgga atacgcggta cacaaaagca
     1321 aggactggga gaagatcaac cgtctcgatc cgagcaaggc tgaggttctt gcggatcatc
     1381 taaaagctat caggctcata cggaaatcgg tcggggacga cctgcctgtg gtcatgacgg
     1441 tattcaaccc gatttcgatt gcgggcgatc tggtcaagga tgatcctttt ctggtggatc
     1501 agatccgcag agatcccgac ccgcttcaga gggcgctgga gagaatcacc gagaccttca
     1561 tagatttttc aaaagcagcg gttgctgcgg gagcggacgg tatcttcttc gccaccactc
     1621 aatgggcctc gaccagcagg attactgagg atgaatacga taaattcggg cgcaaatacg
     1681 attttatgct cctggatgag gttatgccgc atacgaagat caacgttttg catgtctgcg
     1741 accgctgtaa tatgctcccg cttttcaagg attatccgat taagatagtc aactgggaca
     1801 tgaatgacga aaccaatccg gacgcggcaa agggggccga gatattgaag gacaaggtca
     1861 tactgggcgg cgtggaacgc aaggggcttt tgcagaaagg gactcatgcc gagctggagg
     1921 cggttgtcga gaagtacaag gccttcgccc agaagcatcc ttttatgctg gggccggact
     1981 gttctattcc ggttactact cccgatgaaa acctgagggt gattaaagca aaaatcgaac
     2041 attaatgcca taaaagccaa ataattgttg ttgatttttg agctgagatt gtggtatata
     2101 cagcgaggat caaaaggtaa ttattttacc catcactaat atagaac
//
LOCUS       DSEB01000276            3817 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002401, whole genome shotgun sequence.
ACCESSION   DSEB01000276 DSEB01000000
VERSION     DSEB01000276.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002401"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(36..740)
                     /locus_tag="ENO07_05055"
     CDS             complement(36..740)
                     /locus_tag="ENO07_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01371.1"
                     /translation="MQPLMDFVAGPLFRLTFAIMLLGLLRIVVLTVWGVIQARRQTDD
                     KVMPWKLITENTLGWLVPVLRLWKTRPIYSVVSFVFHIGLILVPIFLIGHIQLIRESI
                     GIGWPVLSDNLADILTIVTMVTAVALFLGRLFSKEARFISRPQDYVWPVLLAIPFATG
                     YVCNNMAIGAGVYQWTMLFHMLSAELIFVFIPFTKIAHCVLVPFSQMVSTLGWKFPPR
                     SGEGVAETLGKKEMPV"
     gene            complement(741..2225)
                     /locus_tag="ENO07_05060"
     CDS             complement(741..2225)
                     /locus_tag="ENO07_05060"
                     /inference="COORDINATES: protein motif:HMM:PF02754.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDS01372.1"
                     /translation="MIKPITEEERQHAFKTQRKPEVLEKQKLDVRYHIEDPVVDGRAQ
                     KFLEAFAAILKNTNYGFVLDQYVRSSAKCSRCATECQVYQATRDPKDIPCYRSELLLS
                     VYRRHFTVGGMLRGRVLGSKGLTDEKVNEMAESFWNCTACRRCTLQCPVGIDHGLVTH
                     LGRYILSEIGIAPRALVISTREQLEGKTGNTSAIPVPALLDTLEFLEEDMEEEKGVKI
                     KFPIDQEDCEYVFFPAVSDFLMEADTLMGNAAVFTATGGSWTIGTGYFDGINYGLFYS
                     DRMLDRIVKKVVEETRRLKGRKILIGECGHASRSAKYFVPVFCGGKDAPPVVNCMEYL
                     YDMLKEGKIKLDPNVITDPVTYHDPCNIARQGWIIDKPREILKSFCKSYVEMTPSGKN
                     NICCGGGGGTVSIDEIRPYRTTIGGKAKAEQIRKTGAKYVVAPCANCKKQLRELMEDQ
                     GMDDVEIVGLHDLVYKAIIFDESLKVKKEEKEEETPEAVEKEVE"
     gene            complement(2270..3010)
                     /locus_tag="ENO07_05065"
     CDS             complement(2270..3010)
                     /locus_tag="ENO07_05065"
                     /inference="COORDINATES: protein motif:HMM:PF02579.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01373.1"
                     /translation="MKLAIATYKDEIAPCFEAAKRFQICLLEEREVISKELLNCDRSG
                     PIARLRLLKDAGVEVLLCNGIRSFYKDMLEAENLMVYKDLTGKIEETLKLFIGGKIKH
                     TGKAEENKEAPCLFELGELVEMTREYLSKNGFVIENDESEFPVDIIATLKCPRCKKPI
                     RVAVCCAGHVFYWEKEIMELRSISENYDAAVYVHAAQDQVVKTCKDFNINLLDPWVLE
                     NPEMGSGKDPLPVFRIPVRGHEAVFDKR"
     gene            complement(3224..>3817)
                     /locus_tag="ENO07_05070"
     CDS             complement(3224..>3817)
                     /locus_tag="ENO07_05070"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00158.24,HMM:PF02954.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDS01374.1"
                     /translation="LQEREYEPLGSTTAIKADIRIVAATNQDLKEQISQGKFRDDLYF
                     RLAVVKFDLPPLSRRREDIPYLVDHFISNFNAKKGKNVIGVSPKVMEILMKHEYNGNV
                     RELENIIEYCFAVTRERIIQPSNLPSEVLEAAGELSQQEQAGKVSSSASKADSEEALI
                     RDTLRECQGSRKKAAEILGIDRTTLWRKMKKYGIEIP"
BASE COUNT          880 a         1049 c          877 g         1011 t
ORIGIN      
        1 gcctatacat ttcgttatat cagtcaggat agctttcata ccggcatctc cttcttacct
       61 aatgtttccg ctacaccctc gccggatctg ggcgggaatt tccatcccag tgtggagacc
      121 atctgcgaga atggcaccag cacacaatgg gcgatcttgg taaacggtat gaatacgaaa
      181 atcagttcgg ccgaaagcat gtggaaaagc atcgtccatt gataaacacc tgctccgata
      241 gccatattgt tgcaaacata tcccgtcgca aacggtatcg ccagaagtac cggccagaca
      301 taatcctggg gacggctgat gaaacgcgct tcttttgaaa agagccttcc cagaaaaagc
      361 gctaccgccg taaccattgt aacgatggtc aggatgtctg ccagattgtc gctcagtaca
      421 ggccagccga taccgatcga ctccctgatg agctgtatgt ggccaatcag gaatatcgga
      481 accagtatca atccgatatg gaagacaaac gaaacaacgc tgtaaatcgg tctcgtcttc
      541 cagagccgca gaaccggtac caaccagccc aatgtattct cggtaatcag tttccagggc
      601 attaccttgt catcggtctg acggcgggcc tgtatcacac cccaaacggt cagtacgact
      661 atgcgcagaa gccccaggag catgatggcg aaggtcagcc tgaaaagagg ccctgccaca
      721 aaatccatta atggctgcat ctactccacc tccttttcga ccgcttccgg agtttcttct
      781 tctttttctt cctttttcac cttgagcgat tcatcaaaaa ttatcgcctt gtaaaccaga
      841 tcatgcaggc cgactatttc gacatcatcc atgccctgat cctccatcaa ttcgcgcagc
      901 tgttttttgc agttggcaca gggcgcgacc acatatttgg cgcccgtctt cctgatctgc
      961 tccgccttgg ccttgccgcc gatagtcgtg cgatagggac ggatttcgtc tatcgaaaca
     1021 gtcccgcctc cgcctccgca gcagatattg ttcttgccgc tgggagtcat ctccacataa
     1081 ctcttgcaga atgacttcaa tatttcacgc ggcttgtcaa tgatccagcc ctggcgggcg
     1141 atgttacagg gatcatgata ggttaccgga tcagttataa cattgggatc cagctttatc
     1201 ttgccttctt tgagcatatc ataaagatac tccatgcagt tgacaaccgg cggagcgtct
     1261 tttcctccgc agaagaccgg cacgaaatac ttggccgaac gggaggcatg accgcattcg
     1321 ccgatcagga ttttcctgcc cttcagcctg cgagtttcct ccaccacctt cttgacaatt
     1381 cgatccagca tccggtcgct gtagaacagg ccgtagttga tgccgtcaaa ataaccggtg
     1441 ccgatggtcc aggagccgcc ggtggcggta aagactgccg cgttacccat cagagtatcc
     1501 gcttccatca aaaagtccga gacagccggg aagaaaacat attcgcagtc ctcctgatca
     1561 atcgggaact tgatcttgac gcccttctcc tcctccatat cctcctccag aaattccagt
     1621 gtatccagaa gggccggaac aggtatggcc gaggtatttc cggttttgcc ctccagctgt
     1681 tcacgggtgc tgatcaccag tgctcgcgga gcaatgccga tctcgctcag tatatatcgc
     1741 cccagatgcg tcaccaggcc gtgatcgatc ccgaccgggc actgcaaagt gcagcgccgg
     1801 caggcggtgc agttccagaa tgactccgcc atttcattga ccttctcatc ggtcagtccc
     1861 ttgctgccca ggacccggcc gcgtaacatg ccgccgaccg taaaatgacg gcggtacacg
     1921 ctcaaaagca gttccgagcg ataacagggg atgtccttgg gatcacgggt cgcctgataa
     1981 acctggcatt cagtggcaca acgcgaacat ttggcgctgc ttctgacata ctgatcgagc
     2041 acaaatccat aatttgtgtt cttcaatatg gccgcaaaag cttccagaaa cttctgcgcc
     2101 ctcccatcca caaccggatc ctcgatatgg taccgtacat cgagcttctg cttctccagt
     2161 acctccggct tgcgctgggt tttgaaggcg tgttgtcgtt cttcctctgt gattggtttg
     2221 atcatcgctt cctcatctat tgttgttgtt cacatttttc catttgattc tatctcttgt
     2281 caaaaactgc ctcatgtcct ctgactggaa tcctgaaaac cggcaacggg tcttttccgc
     2341 tgcccatttc cggattctcg agcacccagg gatcgagcaa atttatatta aaatccttgc
     2401 acgttttcac cacctggtcc tgtgccgcat gcacatagac agcagcatcg taattttcgg
     2461 atatggatct cagctccata atttcctttt cccagtagaa gacatgcccg gcgcagcaaa
     2521 ctgcaacacg gatcggcttt ttgcagcggg ggcatttcaa agtcgcgatt atatccaccg
     2581 ggaattcgga ttcatcattc tcaattacga acccgttttt ggataaatat tcgcgggtca
     2641 tctccaccaa ttcccccagt tcaaacaggc agggagcctc tttattctct tccgcttttc
     2701 cggtatgctt gattttgccg ccgatgaaga gcttcaatgt ctcctctatt tttccggtta
     2761 agtctttgta taccatcaga ttttccgcct cgagcatatc cttgtaaaag gacctgatgc
     2821 cgttgcatag aagcacctca actcccgcat ccttgagaag tctcagacgg gctatagggc
     2881 cgctgcggtc acagttcagc agctcctttg agatcacttc cctttcttcg aggaggcaga
     2941 tttgaaatcg cttggctgct tcgaagcagg gagcgatctc atccttgtat gttgcaatcg
     3001 ccaatttcat aaccagaagg gtatatatat gcgaaacatg tgccaaaatt cacaaattag
     3061 cttagtcgtt gttagacaaa ttattacata aaagggcaag aaccgccatg ttgcaataat
     3121 cataaattgc aacgtaatgt tgcagggtgt tcgttgcatt atgcagcata aaggcttatg
     3181 gggaggtgat tagtgcttcg gaaattaagt gcggttttca ggcttacgga atttcaatcc
     3241 catacttttt catcttgcgc cagagggtgg tgcggtcgat gcccaatatt tcggctgctt
     3301 ttttgcggct gccctggcat tcccggagcg tatcgcgaat caaagcttct tcgctgtcgg
     3361 cttttgaggc ggagctgctg acctttccgg cctgttcctg ctgtgacagt tcgccggcgg
     3421 cctccagtac ttcggatggt aaattcgagg gctgtataat ccgttcgcgc gtaaccgcaa
     3481 agcagtattc gattatattc tccagctcgc ggacattgcc attatactcg tgcttcatca
     3541 gtatctccat caccttgggc gaaacgccta taacattttt gccttttttg gcgttgaagt
     3601 tggagataaa atgatccacc aagtagggga tatcctcgcg gcgcctggaa agaggcggca
     3661 aatcgaattt gaccaccgcc agccggaaat agagatcgtc acgaaatttc ccctgtgaga
     3721 tttgttcctt caagtcttga ttggtggcag ccactatcct gatgtccgct tttatcgctg
     3781 tggtcgagcc cagcggctca tattctctct cctgcaa
//
LOCUS       DSEB01000277            3456 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003017, whole genome shotgun sequence.
ACCESSION   DSEB01000277 DSEB01000000
VERSION     DSEB01000277.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003017"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(105..986)
                     /locus_tag="ENO07_05075"
     CDS             complement(105..986)
                     /locus_tag="ENO07_05075"
                     /inference="COORDINATES: protein motif:HMM:PF13527.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDS01375.1"
                     /translation="MNNYRIHKNYRDDDILRNAFFDFIETVYPGLNFKIWFEKGFWRD
                     EYDPHSIMIDGRIVSNISTTRMNLFVDGKQVDGIQIGTVGTIPEYQRQGLSRTLMEHV
                     LDKYKGKTEIIFLYSSEEAFKFYPKFGFDRYNEVIFIQSKDIPKGRFSARKLDINSVA
                     DFELIKKWLSRRSIISKLFGASDYDFITMWHILNRYPANLFYLEEDEIIFIASEKNSQ
                     LHILDIIYHRQFDLTGALSRIIESNNLKSVHYYFSPDQLDFKYDEVVIDTDSPLFVRG
                     KFPIGGRHFKFPATAQT"
     gene            complement(1136..3205)
                     /locus_tag="ENO07_05080"
     CDS             complement(1136..3205)
                     /locus_tag="ENO07_05080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HDS01376.1"
                     /translation="MRNKQLLSCLKSFSSSLLLLFFVAFTTAASAQVPLDPLTLTKYV
                     DPLPVPGVMPQAAPNHYEIGMWEVSQQLHSELPPTTVWGYGISQATASFPGATIEAVR
                     GVPVTVDWVNNLPMTHLLDYAIDTTLHMAEWTSGVPTVVHLHGGEVEPQSDGGPDAWF
                     TQGFAETGPGWYKETYEYANEQLPTTLWYHDHVLGLTRLNVYAGLAGFYLLRDPAIED
                     PLNLPSGEYEIPMVIQDRMFNVDGSLHYPVGGDNPEIHPLWQPEFFGNTILVNGKVWP
                     YLEVEPRKYRFRILNGSNSRFYAMEIWEDVADTTGPGFWQIGSDGGYLDAPVLISDPN
                     DPNSPRLVIAPGERRDVIVDFSGYAPGTTFTLRNNARSPYPNGMTVNPLTDGQVMQFR
                     VVELTAPDNSSIATPGPGYVEKLTTWDNTRQLTLDEIMGPNGPIMAILNNTTWDAPTT
                     ETPELGSTEVWKIINTTGDAHPIHLHLVQFQLMSRQKFQANKYAKVYQSGQNAPAPDI
                     TPYLIGQPEPPEPGEDGWKDTYIMLPGEVTTVIARFAPQDGAPDYSFDATAEPGYVWH
                     CHILEHEDNEMMRRLKVMPAMGMFAAKSDVDGNRAPASFGLDQNYPNPFNPSTEISFS
                     LSEQSHVKLEVFNILGQNVSTLADEEYSAGRHTVTWDASQQASGVYFYRISTKDGVDA
                     RRMMLLK"
BASE COUNT          907 a          801 c          849 g          899 t
ORIGIN      
        1 ataaaatacc atgtataata agattgtcaa tttaaatggt acatcacaaa gaaatattgg
       61 aaaagccaga cctgaaatat gaccattttg agccactttt aaaattaagt ctgtgctgtg
      121 gccggaaact tgaaatgcct tccgccgatc ggaaactttc cccttacgaa aagcggcgag
      181 tcagtgtcta tgacgacttc gtcatattta aaatcaagct ggtcagggga gaaatagtaa
      241 tgaacggact ttagattgtt cgattcaatt atcctggaaa gtgcccctgt taagtcgaac
      301 tgccggtggt agattatatc caggatatgt aattgtgaat tcttttctga ggcgataaaa
      361 attatctcat cttcctccag ataaaacagg ttggccggat accggttgag aatatgccac
      421 atggtgatga agtcataatc ggatgccccg aaaagtttgg atattattga acgcctgctt
      481 agccacttct ttataagctc gaaatcagct acagaattga tatcgagctt ccgcgccgag
      541 aatcgtcctt tcgggatatc tttagattgt atgaatataa cctcattata tcgatcaaat
      601 ccgaatttgg ggtaaaactt aaatgcctcc tcgctcgaat acagaaatat tatttcagtc
      661 ttgcctttat atttatcgag aacgtgctcc atcaatgtcc tcgataatcc ctgtctctgg
      721 tattccggta tggttcccac tgtgccgatc tgaattccat ctacttgttt gccatcaaca
      781 aagaggttca ttcttgtggt ggaaatgttc gatacaatcc tgccatcgat cataatcgaa
      841 tgagggtcat attcatctcg ccagaatccc ttttcaaacc agattttaaa attgagtcca
      901 ggatagactg tctcgataaa atcaaagaag gcgtttctta aaatatcgtc atccctgtaa
      961 tttttatgga tcctgtaatt attcatgtta tttcccggct gccgttatcg caaaaaatct
     1021 ctcaaagata agcgcaaact tgatctatat aatatattat atagtactag ggcacaataa
     1081 aaaaagaggt accggtcaga accggcacct ctcgttttat aaatgtctac tttgattact
     1141 taagtagcat catgcgtctg gcatcgacac catctttggt ggagatgcgg tagaagtata
     1201 cgcctgatgc ctgctgcgat gcatcccagg taacagtgtg acgaccggca gagtattcct
     1261 catcggccag tgtcgaaaca ttctgcccga gaatgttgaa gacttccagc tttacgtgcg
     1321 actgttcgct taagctgaaa gatatctcgg ttgaaggatt gaatggattc gggtaattct
     1381 ggtcgagccc gaatgaagcg ggggcccggt taccgtccac atctgacttg gccgcgaaca
     1441 tacccattgc cggcataacc ttgaggcgac gcatcatttc attgtcttca tgctcgagga
     1501 tatggcagtg ccatacatat cccggctcgg cagtggcatc gaaactgtaa tcaggtgcgc
     1561 catcctgggg agcgaagcgg gcgatcacag tcgtcacttc accggggagc atgatatagg
     1621 tgtccttcca gccatcttct cccggttcag gcggctccgg ctgcccgatc agatatggag
     1681 taatatccgg agcgggagca ttctgacccg actgataaac cttggcgtat ttatttgcct
     1741 ggaatttctg gcggctcatc aactggaact gtaccaggtg caggtggatc gggtgagcat
     1801 caccggttgt attgattatc ttccagactt ccgtgctgcc cagttccgga gtctcggttg
     1861 tgggagcatc ccaggtggtg ttgttgagaa tagccataat gggaccattg ggccccataa
     1921 tctcatcaag cgttaactgg cgggtattgt cccaggtggt cagtttctca acatatcccg
     1981 gcccgggagt ggctatcgag ctgttgtccg gagcggtgag ttcaaccaca cggaactgca
     2041 tcacctggcc gtcggtcagc ggatttacag tcataccatt gggatatggc gaccgggcat
     2101 tgttgcgcag agtaaaggtt gttcccggcg cgtagcctga gaagtcgaca ataacgtcac
     2161 ggcgttcgcc gggagcgatg acgagtcttg gcgagtttgg atcattggga tctgaaatta
     2221 agaccggcgc atccaggtag ccgccgtcgg aaccgatctg ccagaaaccg gggccggtcg
     2281 tatcagccac atcttcccat atctccattg cataaaagcg cgaattggag ccgttcagga
     2341 tccggaaacg atatttgcgg ggctcaacct cgaggtaggg ccagactttg ccattgacca
     2401 ggatcgtgtt gccgaaaaac tccggctgcc agagcggatg aatctcagga ttatctccac
     2461 ctactggata atgcagggaa ccgtcgacat tgaacatgcg atcctgaatt accatcggaa
     2521 tctcgtattc acctgagggc agattgaggg gatcctctat ggccgggtcg cgaagaaggt
     2581 agaatccggc taatccggcg taaacattga gacgagtcag gccgagcaca tgatcgtggt
     2641 accagagagt ggtcggaagc tgctcgttgg catattcata ggtctcctta taccagccgg
     2701 gtcctgtctc cgcaaaaccc tgggtgaacc acgcatcggg gccgccatcg ctctggggct
     2761 caacctcacc gccgtgcaga tgaaccacgg ttggtacacc ggaagtccac tcggccatgt
     2821 gcagagtagt atcgatggcg taatccagaa gatgcgtcat gggcaggttg ttgacccagt
     2881 ccactgttac cggtacgccg cgcactgcct cgattgttgc tccggggaat gacgcggtgg
     2941 cttgagaaat accataaccc cagactgtcg taggcgggag ctcgctgtga agctgctgac
     3001 ttacttccca cattccgatt tcataatgat tcggtgcggc ctgcggcatc acgcccggca
     3061 caggcagggg atccacatat tttgtaagtg taagcggatc aaggggaacc tgggccgaag
     3121 ccgcagttgt gaaagccaca aaaaagagga gaagaagcga agaactaaaa cttttcaaac
     3181 aactcaatag ctgtttgtta cgcatggtag cctcctaagc tctaattaaa aattggcaga
     3241 ttcgaatgaa cctgctgttg acatggaaaa caaaacaaat tttatcttct tttagttccg
     3301 agttcttcac aaattcagaa aatatttctc aggctgtcgt caacaggcaa tgatattaat
     3361 gatagtgtat aaaagaagac tcattaattg ataccttgac tatttgttaa ttccggaagc
     3421 gaataagaaa gtggatccga ttcactatag agttag
//
LOCUS       DSEB01000278            2338 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1007164, whole genome shotgun sequence.
ACCESSION   DSEB01000278 DSEB01000000
VERSION     DSEB01000278.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1007164"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..819)
                     /gene="rfbB"
                     /locus_tag="ENO07_05085"
     CDS             complement(<1..819)
                     /gene="rfbB"
                     /locus_tag="ENO07_05085"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDS01377.1"
                     /translation="MSDLDKFEKRILITGGAGFIGSNLLHYMLIKYPRYLFVNLDKLT
                     YAGNLSNLQDVEMKENYIFARGDIADRDLVSELFQVYQFDGLINLAAETHVDRSILGS
                     GRFINTNIIGTHNLLEISRKHNHFGQEFRFHQVSTDEVFGSLGDTGKFSEKSCYAPNS
                     PYAASKASADHFVRAYHKSFGLNVVTSNCSNNYGPYQFPEKIIPLMINNALHHQPLPV
                     YGDGGNIRDWLYVADHCKAIDIVFHKGRTGKTYNIGADKEIRNIDLVRMICRIMD"
     gene            1056..2279
                     /locus_tag="ENO07_05090"
     CDS             1056..2279
                     /locus_tag="ENO07_05090"
                     /inference="COORDINATES: protein motif:HMM:PF13229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01378.1"
                     /translation="MKKIISATLYLLFSAIAAVASVLHVPDQYGSINQAVSSAQNGDT
                     VLVSPGIYFEHIDFNGKGIMLASNFILDSNLNTVKGTIIRGFDQMHPDTGTVVTFRSG
                     ETSSSILTGFTIRSGFGTLIADSYLGGGILCMNGSNPIICNNIIRENNAINGGACAFL
                     NSDPYFCHNTVIYNNALRGGGMYLEEAGAIIEHNIFADNSATTDGGGIYILLSDSSVI
                     KNSVIFNNNAAATGGISCVSCCPNISFNNLYNNQQADFGDCREGFGDTTVCLNFNLVP
                     SDSFFNFSRHPMFMDTIGADFYPQGQSLLIDAGSETTADFPWNGLRTDIGIYEVHYLV
                     GDSNGDKQLNISDVFFLIKYVFQRGPVPDPFYSGDFNCDRRLNLSDIMEMMNYIFANG
                     FGPCANVDWPLESAH"
BASE COUNT          611 a          527 c          558 g          642 t
ORIGIN      
        1 atccatgatt ctgcatatca tcctgaccag gtcgatattt ctgatttcct tatcagcgcc
       61 gatattgtag gttttacccg tccgtccctt gtgaaatacg atgtcgattg ctttgcaatg
      121 atcggccaca tacagccagt cgcggatatt tccgccatcg ccgtaaaccg ggaggggctg
      181 atgatgaagg gcgttgttta tcatcagcgg aatgatcttt tcaggaaatt gatagggacc
      241 gtagttgttt gaacagtttg atgttacaac attcaggccg aagcttttgt gataggcccg
      301 gacgaagtga tcggcggacg ctttggaggc ggcatagggc gaattgggag cgtaacagct
      361 cttctcgctg aatttgccgg tatcgcccag cgacccgaag acctcgtcgg tcgagacctg
      421 atgaaagcga aactcctgac cgaaatgatt atgtttgcgg gaaatctcca gaaggttgtg
      481 ggtgccaatg atattcgtat ttatgaatct gccggaaccc aggattgagc ggtcgacatg
      541 ggtctccgcg gccaagttta tgagaccatc aaactgatac acctggaaaa gctcgctcac
      601 caaatccctg tcggcgatat cgccccgggc gaatatgtag ttctccttca tttcgacatc
      661 ctgcaagttg gagagattgc cggcataggt aagtttgtcc aggtttacaa agagataccg
      721 gggatatttg atcagcatat aatgcagcag gttcgagcct ataaaaccgg ccccgcctgt
      781 aatcaaaatc cgcttttcaa atttgtctaa atcgctcata ttggatggtc atccccagct
      841 ttttcaagcc gaggaataat actgttcttc cggcagtttg tcaagcctga gagatatgtc
      901 cgcagatgat aagttcaaat agaaaatcag ggagttttcg ttaaaacatt aacttgtatt
      961 tccgcaaaaa acaattataa tagtttatca gcaccagggg gttagaactg acccatgaaa
     1021 aggtctaact tataacctgt tttgtgctgt agtttatgaa aaaaataata tcggccaccc
     1081 tgtatttact gttttccgca attgctgccg tggcaagtgt tctgcatgtc cccgaccagt
     1141 atggtagtat caatcaggca gtttcgagcg cccagaatgg ggatacagtt ttggtgtcac
     1201 cgggaatcta tttcgagcat atcgatttca acggtaaagg catcatgcta gcatccaatt
     1261 ttatcctcga ttccaattta aacactgtaa agggcacgat tatcaggggt tttgatcaaa
     1321 tgcatcccga tacggggaca gtagttacct tcagaagcgg agaaacaagc tcctccatct
     1381 taactggatt tactatccgg tccggtttcg ggacgcttat agcagacagc tacctgggcg
     1441 gcggcattct atgcatgaac ggttccaatc cgatcatctg taacaatata atcagggaga
     1501 acaatgctat caatggagga gcatgcgctt ttctgaattc cgacccatac ttctgtcaca
     1561 acactgtaat ttataataat gctctgcggg gcgggggcat gtatctggag gaagccgggg
     1621 caattataga acacaatatt ttcgccgata attccgccac aacggatggg ggagggattt
     1681 acattttact cagtgacagc tcggtaataa agaattccgt gattttcaac aataatgctg
     1741 ctgccacggg cggaataagc tgcgtctcgt gctgtccgaa catctccttc aacaatcttt
     1801 ataacaatca gcaagccgat ttcggcgact gccgcgaggg tttcggcgat accacggtgt
     1861 gcctgaactt taacctggtc ccctccgata gttttttcaa tttcagccgg catccgatgt
     1921 tcatggatac gattggagcg gacttctatc cccagggtca atcgttgctg attgacgccg
     1981 gctcagaaac taccgcggat tttccctgga acggcctgcg taccgatata ggtatatatg
     2041 aagttcatta tctggtggga gatagtaatg gcgataagca gcttaatata tcagatgtat
     2101 tctttttgat aaaatatgtt ttccagagag ggcctgtgcc agatccattc tacagcggag
     2161 atttcaattg cgatagaagg ctcaatctca gcgatataat ggagatgatg aattacattt
     2221 ttgcgaacgg cttcggtccc tgcgccaatg ttgactggcc gcttgaatca gctcattgac
     2281 cttgcagaat ctgccttccc gcttaatctg tcagcgccgt agaatataaa agcaactg
//
LOCUS       DSEB01000279            2361 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1007007, whole genome shotgun sequence.
ACCESSION   DSEB01000279 DSEB01000000
VERSION     DSEB01000279.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1007007"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..643)
                     /locus_tag="ENO07_05095"
     CDS             complement(<1..643)
                     /locus_tag="ENO07_05095"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HDS01379.1"
                     /translation="MMKKIIAISILLSLALSSGALADDGNAGGPAAFLKLGFSARVMG
                     MGGTYLAISDDATGMFYNPAGLAQLKWNSASASYRHMDFDRKFGYVAFGVQALEEATI
                     AGGWIHASDGNFEGRDNEGALTGEELSYSDNTVALSFAKKFGEIFMVGATGKYFISKV
                     ADITSNTVSFDLGAMAVLDKLNFFQPDAFFDLVRIGVVVENMGGTHRFNTGDYW"
BASE COUNT          593 a          679 c          543 g          546 t
ORIGIN      
        1 accagtagtc accggtgtta aagcggtgcg tcccgcccat attttcaaca acaacgccga
       61 tacgtaccag atcaaaaaac gcatccggct ggaaaaagtt caatttatcc agcacggcca
      121 tcgcacccaa atcgaaactc acagtattgg aggttatatc ggcaactttg cttataaaat
      181 atttaccggt tgccccgacc ataaaaattt cgccgaattt cttggcaaaa gacagggcga
      241 cagtgttatc ggaatatgat aattcttccc cggtaagtgc gccttcgtta tcgcggccct
      301 caaaattgcc gtcggaagca tgaatccagc cgcccgcaat ggtagcctcc tccagagcct
      361 ggactccgaa ggcaacatat ccgaatttgc ggtcgaaatc catatgccgg tatgaggcgc
      421 tggcagaatt ccacttaagc tgcgccagcc cggccggatt atagaacatg ccggtggcat
      481 catcggaaat tgccaggtag gttcccccca tacccatcac ccgagccgaa aaaccgagct
      541 tgagaaaggc cgccggtccg ccggcattac cgtcatcagc gagcgctcca gaagataggg
      601 ccagcgataa taatattgag atagcaatta ttttcttcat catacactcc tgcctacggt
      661 attaccatta ttttgcccca ggcctcttct cccgaggaat tttcgacctt aaagtaatac
      721 ggtccggtag cgactatatc accattacca tttcgcatat cccatgaatc cccgtcatgc
      781 tgaactccgc cttctctttg ttcaccgtca aggggagtgg ccacaaggtc catggcgaag
      841 tcatatacct ttattgtcac gcttccgctc tgatcgaacc tgtaatgaaa ttttacacgt
      901 ccgccgggag agacgaaggg ggatgatggt accggagagg catatgctgt aggaccggcg
      961 tcctcgggaa tatcctcaaa ggtcctgtaa accgcccagc tttctcctcc gtccgtgctt
     1021 tttgccatgc catcgttgga ggctgcccag atcacgccgt cggcaagacc gactccaaag
     1081 aactccgtgc cctcatatat cctctcgcct gtggcagcat caaccagctc cagcgtgtcc
     1141 caggtctcac cgaaatcgga ggtgcgcaga agaccgatgg aggtggccgc atagacatcg
     1201 gcgccgtcaa aagcgaagtt ccatgtattg ataccggtca aggcaatctt ccaggttcga
     1261 ccattgtcgg ttgagacatt aatgccgttg ctttctccca aaaggccggt ggagttccct
     1321 gctacccaga catatctttg accatcatag tactggactt caacagaaac cgcaaaattt
     1381 cccgaaagtc cctcttcatc tcccgaggtg gcacgaatat tatgatcgaa ttttgtctga
     1441 tccagattta ccgtgtcgat actccagagg ccgggcagag cactgccgct ggatgccagg
     1501 ccttcgactg aaccgaccca gaaaagggaa tcgatatcat tgaacagaag agatgtcatg
     1561 gttgtgtcga tgatagtctc ctcttgcgcg tcacttactt caacaggaat ccaggtcgca
     1621 ttcaaacgat tatatctttg cagcccgttg cggcttgcca gccagatggt aaaatcggta
     1681 tagatcagat cattgaccgt ctcaccaaca aatgttgttg tccaggtctg accgaaatcc
     1741 tgtgagagca ggacggcgag gccgccctgg ccgtcatacg gccgcgtagc catagcccat
     1801 tcgtaaattg tatcaaccac atcaccttct tcttcctccc agaagttagc gcccacagcc
     1861 tcaaccgcca atcccgcgtt ctcgatgcca tcaaacctga ataactcggc attcataaca
     1921 gcaccatcga aggggcatgg aatctccaat ttccacaggc ctcccgcgct cccggcgagg
     1981 acatataatg tatcatcacc ggtgcagggc tcctggtccg atagtgaata aaaactcgcc
     2041 agtgaaagaa tctggttaaa cgatgaatcc ggagaggttt cggccgcatt cagggtgatc
     2101 ttctcccagg tctgactatc atcctgcgaa cggatcaatc cgccctcggt acaggcggcc
     2161 cagattgacc tgctgccatt atcgtccaga gtgattaaaa tatcctcgac ttttttacct
     2221 gcgcccacag cttcaacagg caggctctgt atccaggttt ctccaaaatc cgcagaatac
     2281 gcaaagccct gactttcgct aaaatcagga tcgtatccgg cgcagatcac gaaatctccg
     2341 cgtgcggccg aagcgctggt g
//
LOCUS       DSEB01000280            2166 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1008498, whole genome shotgun sequence.
ACCESSION   DSEB01000280 DSEB01000000
VERSION     DSEB01000280.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1008498"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1109)
                     /gene="guaA"
                     /locus_tag="ENO07_05100"
     CDS             complement(<1..1109)
                     /gene="guaA"
                     /locus_tag="ENO07_05100"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDS01380.1"
                     /translation="MIEEILILDFGSQYTQLIARKIRELHVYCEIVPFNFPIEEARSK
                     NLKGLVLSGGPASVLADDAFFCDPAYFDMGLPILGICYGLQLIGITMGGELQRSRSRE
                     YGPAMLTPTETSLLFDDIEEPSRVWMSHGDSLDKLPPGFINIGRTSQVEHSAIANRDK
                     KIYGLQFHPEVYHTQNGKQILSNFLFKICKAAGDWTMESFIEDSVKRIREMAGRSRVL
                     CAVSGGVDSSVCALLLSKAVGKNLVAVFVNNGLLRKDEDTAVIKELADLNVDIRAVDA
                     SDIFLSRLKGVIDPEEKRKIIGRTFIEIFDEKAKELGQIDFLAQGTLYPDVIESVSFK
                     GPSATIKSHHNVGGLPEKMDLKLIEPLRELFKDEVR"
     gene            complement(1114..>2166)
                     /locus_tag="ENO07_05105"
     CDS             complement(1114..>2166)
                     /locus_tag="ENO07_05105"
                     /inference="COORDINATES: protein motif:HMM:PF02910.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDS01381.1"
                     /translation="VYKLLASITLIATGGIGQVYLHTTNPKIATGDGVAMAYRAGAKI
                     ANLEFMQFHPTALFHPEGEGFLISEAVRGEGGRLRLRTGKRFMKNYDPRMELATRDVV
                     ARAIDREIKLSGDPCVFLDLTHLKESFIRQRFPNIYGKLKNLGIDIAVDWIPVVPAAH
                     YMCGGIDVDLEGRSSIENLYVVGEAAHTGMHGGNRLASNSLLEAVVFAEIAAGSARKE
                     LENHAGNNEMVSEFDRVMAGKIHVSEEEKILIAHLMLDVKQVMSNYVGVVRSNKRLNW
                     ARRRLEIIKNDISDLWQDFPLSYDLIELRNLAAVAELIVECAQIRKESRGLHYNIDYP
                     DQDDKNWKKNTIIINN"
BASE COUNT          522 a          592 c          499 g          553 t
ORIGIN      
        1 cgcacctcat ccttgaacag ttcgcgcaaa ggttcaatca gcttcaagtc cattttctcg
       61 gggagcccgc ccacattatg atgcgacttg atcgtggccg acggcccctt aaatgatacc
      121 gattcgatca catcgggata aagggtcccc tgtgcaagaa agtcaatctg tcccagttcc
      181 ttagcttttt catcaaaaat ctctatgaag gtgcgtccga tgatctttct cttttcctcg
      241 gggtctatca ctccttttaa acgggaaagg aagatatcgg aggcgtcaac cgcgcgaata
      301 tcaacattca gatcggcaag ttctttaatg accgcggtgt cctcatcctt gcgcagaagg
      361 ccgttattga cgaaaaccgc caccagattc tttcctaccg ctttcgacag caataacgca
      421 cagaccgacg aatcaacccc tcccgaaact gcacataaaa cccggcttct gccagccatt
      481 tccctgattc gtttgaccga gtcctcaatg aaggattcca tggtccagtc tcccgcagcc
      541 ttacagattt taaacagaaa attcgaaagg atctgcttgc cgttctgggt atggtaaacc
      601 tccggatgga attgcaggcc atagattttt ttgtcccggt tggcgattgc tgaatgttcc
      661 acctgtgagg tcctgccgat attgatgaat cccggaggca gcttgtcgag gctgtcgcca
      721 tggctcatcc agacccggga aggctcctct atatcgtcga aaaggaggga tgtttcagtt
      781 ggagtaagca tggccgggcc gtattcacgt gaacgcgagc gttgcagttc gcctcccata
      841 gttatcccga tcaattgcag accataacat atccccagaa tcgggagacc catatcgaaa
      901 taagctggat cgcagaaaaa ggcatcatcg gcgagaaccg atgcgggccc gccggaaaga
      961 accaggccct tcagattttt gctgcgggcc tcctcaatcg ggaaattaaa cggtactatc
     1021 tcgcagtaaa catgcaattc cctgattttg cgcgcaatta gctgagtgta ttgcgagccg
     1081 aaatccagta ttaatatctc ttctatcatc gcttcagttg ttaatgatta ttgtattttt
     1141 cttccaattt ttgtcgtcct gatcggggta gtctatatta taatgaagac cccgggattc
     1201 ctttcttatc tgcgcgcatt ctacaatcaa ctccgccacg gccgccaggt tccgcaattc
     1261 aatcaaatcg tatgaaagag gaaaatcctg ccagagatcg gaaatatcat tcttgatgat
     1321 ctcgagtctt cggcgggccc aattgaggcg cttgttggaa cgcaccacac cgacatagtt
     1381 cgacataacc tgtttgacat caagcatcag atgtgcaatc agaatctttt cttcttcaga
     1441 gacatgaatc ttgccggcca tgacccgatc gaattccgaa accatttcat tatttccggc
     1501 atgattttcc aattcctttc tggctgatcc ggcggcaatt tcggcaaaca caaccgcctc
     1561 cagaagcgaa ttcgaggcca gacggtttcc tccatgcatg cctgtgtggg cggcctcacc
     1621 gaccacgtag agattctcaa tactgctgcg gccctcgaga tccacgtcaa ttcctccaca
     1681 catatagtgg gcagcgggca caaccggaat ccagtcaact gcgatatcaa tccccaggtt
     1741 tttcagcttg ccgtagatat tcggaaagcg ctgcctgata aaactctctt tcagatgggt
     1801 caggtccaga aaaacacagg gatcgcctga gagctttatc tcgcgatcta tggcccgtgc
     1861 cacaacatct cgtgtggcca gttccatgcg tggatcatag ttcttcataa accgctttcc
     1921 ggttctcaga cgcagcctgc cgccctcgcc gcgtactgcc tcggaaatca ggaaaccctc
     1981 accttccggg tgaaataatg cggtgggatg gaactgcatg aattccaggt ttgcaatttt
     2041 ggctcctgcc cggtaggcca tagccacgcc atccccggtc gctattttgg gattggtggt
     2101 atgcaggtat acctgaccga tcccgccggt cgctatcagg gtaatcgagg ccaggagctt
     2161 gtagac
//
LOCUS       DSEB01000281            3261 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003426, whole genome shotgun sequence.
ACCESSION   DSEB01000281 DSEB01000000
VERSION     DSEB01000281.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003426"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..568
                     /locus_tag="ENO07_05110"
     CDS             <1..568
                     /locus_tag="ENO07_05110"
                     /inference="COORDINATES: protein motif:HMM:PF04402.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF541 domain-containing protein"
                     /protein_id="HDS01382.1"
                     /translation="FEIETRDKDLEAAKKVNDEKLEKVKEIFKLFTIASEDIKVSSFK
                     VYPHYDYEDGKIKRFEISREVEVTLREINKYEPLIDALIEAEVYDIGGLSYELSNSAE
                     LNKLARENALDQARNKAREMAEYYGMKLGEVIYISEGSSPSFFNSFGFGASQARVNTL
                     TMPSDSPMILKGRKTIKASIIVKFELID"
     gene            586..1953
                     /locus_tag="ENO07_05115"
     CDS             586..1953
                     /locus_tag="ENO07_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDS01383.1"
                     /translation="MISLRILIVDDEKSQREMLGDFLSKKGYRVETAGSGEEALKKYE
                     GGNFEIALLDQKMPGMDGIELLGRLKEIDPELQAILITAHGTVETAVKAMREGAYHYI
                     TKPIVNLDELLELIRRAGEKHFLLKENKILKEELKENLGDFSIIGDSKAMRKVLSTVS
                     SVAAADTTILLTGESGTGKELVAHAIHGKSPRAENRFVTINCAAIPENLLESELFGHT
                     KGAFTGATSTREGKFSMADHGTLFLDEIGEIPVNIQVKLLRAIEEKSFEKVGGNTPIN
                     VDVRIIAATNRDLRAEMEKGNFREDLFYRLNVINLELPPLRDRREDIMPLVDHYAEHF
                     SRKLNKKVSGITPEAKDLLLRYPWPGNVRELVNVIERAIVLLRGDVIDASDLPLEVEE
                     KPARADKYFTEGEIPSIQEVEREHIKKTLDFTDWNFNKTADILGIHRNTLRLKIKEYN
                     LSKPE"
BASE COUNT          908 a          746 c          836 g          771 t
ORIGIN      
        1 atttgaaatc gaaactcggg ataaagacct cgaggctgcc aaaaaagtga atgacgaaaa
       61 gcttgaaaag gtaaaagaga tttttaagct ctttacgatc gcgagtgaag atattaaagt
      121 ctcgagcttc aaggtatacc cgcattatga ttatgaggat ggtaaaatca aaagatttga
      181 aatctcaagg gaggttgagg taacattgag ggaaatcaat aagtatgaac ccctgattga
      241 tgctttgata gaagcggagg tttatgatat cggagggctg tcctatgaac tctcaaatag
      301 tgctgaatta aataagctag ccagggaaaa tgcacttgac caagccagaa acaaagccag
      361 agaaatggct gaatattatg gcatgaaatt aggcgaggtc atatatatta gcgaaggaag
      421 cagcccttca ttttttaatt cctttggttt tggagcatcg caagccaggg ttaatacatt
      481 gactatgcca tccgatagcc ctatgatatt aaagggtcga aaaaccataa aggccagcat
      541 cattgtcaaa ttcgaattaa tcgactgaag aatgagatat tatctatgat ttccttgcga
      601 atactgattg tggatgacga aaaaagccag cgcgaaatgc tgggcgattt cctctcaaag
      661 aagggctacc gggtcgagac cgccgggtcg ggagaagaag ccctgaaaaa gtatgagggc
      721 ggaaacttcg aaatagctct tcttgatcaa aagatgcccg gcatggatgg gatcgagctt
      781 ctgggcaggc tgaaagaaat agatccggaa ctgcaggcta ttcttatcac ggcccatggc
      841 actgtggaga cggctgtgaa ggcaatgcgc gagggagctt atcattacat aacgaagccg
      901 attgtaaatc tcgatgagct cctggaactg attcgcaggg cgggggagaa gcacttcctt
      961 ttgaaagaga ataaaatcct gaaggaagag ctgaaagaga acctggggga tttttcaatt
     1021 ataggcgaca gcaaagccat gcgcaaagta ttgtcgactg taagttccgt ggcggcggct
     1081 gatacgacaa tactcttgac aggcgaatcc gggaccggaa aagaattggt ggcgcatgct
     1141 atccatggca agtctcctcg cgcggaaaat cgttttgtca ccatcaactg cgcagccatt
     1201 cccgaaaatc ttctggaatc cgagctgttc ggacacacaa aaggcgcatt tacaggggct
     1261 acttctaccc gtgagggtaa attctccatg gccgatcacg gcactttatt tctggatgag
     1321 atcggcgaga tcccggtgaa tattcaggtc aaacttctac gcgcaatcga ggaaaaatct
     1381 ttcgagaagg tggggggaaa tactccgatt aatgttgatg tacgaataat cgccgccacc
     1441 aatcgcgacc tgagagcgga gatggaaaag ggtaatttca gggaggacct gttttatcgc
     1501 ttgaacgtaa taaatctgga actgcctccg ttacgtgacc gccgcgagga tattatgccg
     1561 ctggtagatc attacgccga acattttagc aggaagctca ataaaaaagt ctccggtatt
     1621 acacccgaag ccaaggatct gctgttacgt tatccctggc cgggcaatgt gcgcgaattg
     1681 gtcaatgtga tcgagagggc aatagtgctt ttgagggggg atgttatcga tgcctccgac
     1741 ctgcccctgg aagtcgagga aaagcctgcc agggctgata aatatttcac tgagggcgaa
     1801 atccccagca ttcaggaggt cgaaagagag catataaaga agactttaga ttttactgat
     1861 tggaatttta acaagaccgc cgatattttg ggcatccaca ggaatactct ccgtttgaaa
     1921 atcaaggaat ataatctctc caaacccgaa taaaaaaatc ccgcccatta ttgcaggcgg
     1981 gattctgcat ttatctataa tattcaatta tcagcagtca ggctcaccgt cgccgtctgg
     2041 atcgcagggc gcgttaccgc tcacgaaaac ataatttata atacttactg catctgacac
     2101 attaacgctg ccgtcgcaat taggatcggc cgcttccagt ggatcggggg cggttgagcc
     2161 caggaaaacg tagttgatta ccatgacagc gtccgagaca ttaacggtct cgtctccatt
     2221 ggaatcaccg cagatatacg gtacgccgat attcaccgtg aattcctgct gatcaatgct
     2281 gcccgcaaca tctacaacct gcgcggtaaa tgtgaaaatg ccgggtccgg gaggtgtgcc
     2341 cgaaagcagg ccctccgagg aaagcgacaa tcccgtgcct tccagatcat tattgaggtc
     2401 ggaccaggtt aatggtgatg ttccgccctc tgactcgagc tgatgagaat atggttccgt
     2461 ttccagtcct tcaggaagga cggaagtggt aatgctgact tcgggattga tgactatctc
     2521 caggaccttc tcatccgatg atgatgtcgc atcgacggcg cgtgcagtga agctgatcgg
     2581 atctgtcgag gagggcgtgc cggatacaat tccatccagg gaaagtgtca ggccggtgcc
     2641 gtcaagccca tcgtcaaaat caaaccagtt caattcgccg gttccgccgg cggcttcaag
     2701 ctgctgctca tacgccattc cgaccgtcca ttccggcatg ctttcggtcg tgatagagac
     2761 cgccggattt atagtgaagt taaactcctg agtatcgaac gctccaacgt tgtcgattgc
     2821 ccgggcatta aaacttatcg gccctgaagc ggccggagta cctgatacaa gtccgttgac
     2881 ggaaacgctt agcccggtac cctccagccc caaactgaaa tcgctccata ccagatttcc
     2941 ggttccgcct gttgcgaaga gctgctcaga atatgctatt cccgtagtcc aatccggcag
     3001 caggacggta acgatattaa cggccggatt aatttcgaac tggtaagtgc tttcatccga
     3061 agctcccgca aaatcggtaa cctcggctgt aaagctgata gtcatttcgg agctgactgt
     3121 tcctgagagt aatccaccgg ccgacaggct caaaccggtt ccatccagat cgccaaattt
     3181 gtctgaccat gactgaggag aggtgccgcc cgtgaattca agttgctgct gatattcttt
     3241 gcctgctgtc cagtccggaa g
//
LOCUS       DSEB01000282            3443 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003041, whole genome shotgun sequence.
ACCESSION   DSEB01000282 DSEB01000000
VERSION     DSEB01000282.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003041"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..658)
                     /locus_tag="ENO07_05120"
     CDS             complement(<1..658)
                     /locus_tag="ENO07_05120"
                     /inference="COORDINATES: protein motif:HMM:PF13517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HDS01384.1"
                     /translation="MFTLDQKNPIGTVGVVVLCFILLFCSSLRSKPVFETRVDYQVGI
                     SPSSIISADLDGDADPDLAVANALSDDISVLINDGDGWYQPAQSYPVGDLPQSVYAAD
                     LDGDTDIDLVSANTESSNVSVLINNGDGTFQTALNYPAAAAPFSVFAADLDGDADADL
                     AVACYDADSVSVLKNNGDGTFQAAVDYVAGDRAETIFAADLDNDLDLDLAVSYSVGDP
                     T"
     gene            complement(884..2158)
                     /locus_tag="ENO07_05125"
     CDS             complement(884..2158)
                     /locus_tag="ENO07_05125"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HDS01385.1"
                     /translation="MASLKEKFASKIPVWGEDIKKILKEKGDVKISDVSVQQAYGGMR
                     GVKGMICDTSLVEPDKGLIIRGIPILELTDKLPEEIFFLLLSGDMPTDDDVKDLQDEY
                     KKRGDVPKYVWDVLKAMPKTSHPMAMFDTAVLVLENESIFRKRYAEGIPKGQYWDAVY
                     EDGLNLLAKLPAIGAGVYRMKYDKGGLIDPDPKLSWGANFAHMMGIDDETFYKAMELY
                     FTLHCDHEGGNVSAFSCHTIASALSDPYYAVSGGLNGLAGPLHGLANQECLKWVLEMM
                     DKLGGGIPTKDQIKDYAWETLNAGKVIPGYGHAVLRVTDPRYTAFLNFGKEHLPDSEV
                     LTTVARIYEVVPDVLKEHGKAKNPWPNVDAGSGAILYHYGITEFEYYTVLFSISRAMG
                     MVAQQVWNRAMMIPITRPKSVGTDWIKKQVGM"
     gene            2439..2768
                     /locus_tag="ENO07_05130"
     CDS             2439..2768
                     /locus_tag="ENO07_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008241681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HDS01386.1"
                     /translation="MSCIFCKIINKEADARVEYEDEDTLVIHDKHPAASTHLLVMPKE
                     HYEKLMDTPPEIMPKLFEAVQRVAKKLGIDEDGFRVIINNGRGGGQVIPHIHIHLLAG
                     GRLPGFH"
     gene            complement(2775..>3443)
                     /locus_tag="ENO07_05135"
     CDS             complement(2775..>3443)
                     /locus_tag="ENO07_05135"
                     /inference="COORDINATES: protein motif:HMM:PF02954.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDS01387.1"
                     /translation="FLDEIGNTSPAIQMKLLRVLDDQVIMPVGSTQTIKVDVRLVAAT
                     NANLEEEVMAKHFREDLFYRLNVFTVQIPPLRERRDDIVLLVDHFVKMYCRKMGIKAK
                     EVNPDALDILLGYEWPGNVRELENTIERSLVVSTSDKITADDLPDKVSGRVLTGHSAV
                     PEVQESVQTGDQGESPTLESIEKAYIFWVLNQTGWNKTRAAKILGIDASTLYRKIDRY
                     DLKQ"
BASE COUNT          896 a          892 c          834 g          821 t
ORIGIN      
        1 ccgtaggatc gccgacagaa tacgataccg ccagatcaag atccagatcg ttatccaggt
       61 ctgcggcaaa tattgtctca gctctatctc ctgctacata gtctacggcc gcctgaaaag
      121 tgccatcccc gttgttcttc aaaaccgaga cactatctgc atcatagcat gccacagcca
      181 ggtcggcatc agcatctccg tccagatcag cggcaaacac tgagaacggg gcagcggctg
      241 ccggataatt tagcgccgtt tgaaaagtac catccccatt attaatcaaa acggaaacat
      301 tgctggattc cgtgtttgca gagaccaggt caatatcggt atccccgtcg aggtcggctg
      361 catacacgga ttggggcaag tcaccaaccg gatagctctg agctggctga taccaaccat
      421 caccatcgtt gatcagaact gagatatcat ccgacaaagc atttgccacg gccagatcgg
      481 gatcagcgtc accatccaga tcagccgaga tgatcgagga gggcgaaatg cccacctggt
      541 aatcgacccg ggtttcaaaa actggtttgg agcgcaagct cgaacaaaac agcaaaatga
      601 aacataaaac aaccactccg actgtcccga tcggattttt ttgatccaga gtaaacatag
      661 atcctcccgg tataaaacgg cttacggctt tatattattg taataataag ttgtagaaac
      721 atatgtagca atcaatcaat ggaaatatat aaacctgagt catattgtca agaaaaaaat
      781 tgagatgaat atacatattt gagatgcgaa agactcggca caaaaaaagc gccctcttct
      841 caagggcgct gtgtatatcg aacagttgta tggtcttaga tgcttacata ccgacctgtt
      901 ttttgatcca gtcggtgccg accgatttcg gacgggtgat cgggatcatc atagcgcggt
      961 tccagacctg ctgcgcaacc atacccatgg cacgcgaaat cgagaacaga actgtgtagt
     1021 actcgaactc ggtgatgcca taatggtaca gtatcgcgcc ggaaccggcg tcaacattcg
     1081 gccatggatt cttggccttg ccgtgctcct tcaatacatc cggaacgacc tcgtaaatgc
     1141 gggccacggt cgtcagcact tcagaatccg gcaaatgctc tttgccgaag ttcaggaagg
     1201 ctgtgtaacg cgggtcggtc acacgcagaa cagcatgacc atatccggga ataactttac
     1261 ctgcattcag ggtctcccag gcataatcct tgatctgatc cttggtcgga ataccgccgc
     1321 ccagcttatc catcatctcc agcacccact tcaggcattc ctggtttgcc aggccatgca
     1381 gcgggccggc cagaccgttc aggccgccgg aaacggcata gtaaggatct gagagagctg
     1441 atgcaatggt atggcatgag aaggccgaga catttccgcc ctcatggtcg caatgcaggg
     1501 tgaaatagag ttccatggcc ttgtagaagg tctcgtcgtc aatacccatc atgtgggcaa
     1561 agttggcacc ccagctgagc ttgggatcgg gatcgatcag gccgcccttg tcatacttca
     1621 tgcggtaaac gccggcgccg atggcgggca gcttggccag aagattgagg ccgtcttcat
     1681 aaacagcatc ccagtactgc cccttgggga taccctcagc ataccttttt ctgaaaatcg
     1741 actcgttttc cagtaccaga acggcggtgt cgaacatggc catcggatgc gaggtcttgg
     1801 gcatggcttt caggacatcc cagacatatt tgggcacatc gccgcgcttc ttgtactcgt
     1861 cctgcaaatc cttgacatca tcgtctgtcg gcatatcgcc ggacagaagc aggaagaaaa
     1921 tttcttccgg aagcttgtcg gtcagttcca ggatggggat accgcggata atcaaacctt
     1981 tgtcaggctc caccagcgag gtgtcgcaaa tcatgccctt aacaccgcgc ataccgccgt
     2041 aggcctgctg gacagaaacg tcggaaatct ttacatcgcc tttttcctta aggatctttt
     2101 ttatgtcctc accccagaca gggatcttgg aggcaaattt ttcctttaaa cttgccatac
     2161 ttcctcctca catattatag gttataattg gttatcttat attcgctctt aaattttgca
     2221 atatagtcac tcgcgcctca aaacgcaacc ctgttgtgaa agaattcaca ataaatgtct
     2281 tacgccctgc actgtcaagc attatttggc tctatcgcat agttttatca gataaaataa
     2341 aggcatcgat aaagagctgc gattaattca gataatgcct tgcaaaatta tcaatccgga
     2401 ttattttaag ataaatttga caaaggggat aaatttttat gtcatgtata ttctgcaaaa
     2461 tcatcaacaa ggaagcggat gccagggtcg agtacgagga tgaggatacg cttgtcattc
     2521 acgacaagca tccggccgcc tcaacgcatc tgctggtaat gcccaaggaa cattatgaga
     2581 aactgatgga taccccgccg gaaatcatgc ccaaactgtt cgaggctgtt caaagagtgg
     2641 cgaaaaaact ggggatcgat gaggacggct ttcgggtgat aataaataac ggccgcggcg
     2701 gaggtcaggt cataccgcac atccatattc atctgttggc cggaggacgg ctgccgggtt
     2761 tccattaagc gaatctactg cttcagatca tagcggtcga tcttgcggta gagggtggaa
     2821 gcgtcgatgc cgaggatttt ggcggccctg gtcttgttcc agccggtctg attcaggacc
     2881 cagaagatat aagctttttc gattgattcc agagtcggag attcgccctg gtctccggtc
     2941 tggacagact cctggacttc gggcacagcc gaatgccctg tcaggacccg gccgctgacc
     3001 ttatccggca gatcatcggc ggtgatcttg tcagaggtcg aaacaaccag agaacgttcg
     3061 atcgtattct cgagctcgcg cacattcccg ggccactcat atcccagcag aatatccaga
     3121 gcatccgggt taacctcttt tgcctttata cccatcttgc ggcaatacat cttaacaaaa
     3181 tgatcgacca gcaaaactat gtcgtcgcgt cgttcgcgca ggggcggtat ctggaccgtg
     3241 aatacattca gacgatagaa caaatcctcg cggaagtgct tggccatgac ctcttcctcc
     3301 agattggcat tggtcgcggc cacaaggcga acgtcaactt taatcgtctg ggtcgatccg
     3361 accggcatga tcacctggtc atccagcacc cgcaggagct tcatctgaat ggcgggcgag
     3421 gtattgccga tttcgtccag aaa
//
LOCUS       DSEB01000283            2798 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004810, whole genome shotgun sequence.
ACCESSION   DSEB01000283 DSEB01000000
VERSION     DSEB01000283.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004810"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..732)
                     /locus_tag="ENO07_05140"
     CDS             complement(<1..732)
                     /locus_tag="ENO07_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01388.1"
                     /translation="MSAILKINNFISAFIDALKSIFYIRLWTPFFIFFLISIAFAYMI
                     LNPFSGPWSGFVTWIGASSLFGGGEAFTHYPQHLLISPSVHSHLNLVLSIIVDSLLTG
                     AAFIIFAGFFRAQKVKFSEAVSRAFSSYHWLVLASAVIYAILYAVQLLLPEIFMQMLI
                     GNPRRMFFFIVALKLFMFLVFSPLVYTIPYIVLKNENFFSAFLNSIRFFLKNIFTTFF
                     AIFITQLVVLPFSLSLDYSGWIADKF"
     gene            complement(749..1999)
                     /locus_tag="ENO07_05145"
     CDS             complement(749..1999)
                     /locus_tag="ENO07_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01389.1"
                     /translation="MTKNIIISVIILSLGFWLACDREIPSDFDLGEFPDAPPAPSNLR
                     IQVGDNILNLSWDMENPASISKYFIYRSSNVQDTVHLYDSSTTTGYVDDGVQNGNRYI
                     YQVSALSTQNIEGSSTAPVSAIAGVFSIAIDNDAEFTSSRDVFINPTAPERTAHIMLS
                     NSEDFAGAKWLNYSNSVSWQLTDGDGEKTVYARFKDSDGNQTAESFSDNITLDTRAEI
                     DTFYISGPASSFEPGDLIHFVLDSGEPGGSARVEISDLGRLDLFDNGSNGDAVAGNGI
                     YKFDYVIPPNTEVAGSSVTGMFTDRAGNEAPDFYLPYLIDITSDIAPDPVALAIQVVD
                     SVTFRLTWTQNENSDFESYRIYRSTSSGVTNIPENLIIIENRQNTTMFTDEGVIFGNT
                     YYYKVYVYDRFGASAGSNEVFAPK"
     gene            complement(2009..2722)
                     /locus_tag="ENO07_05150"
     CDS             complement(2009..2722)
                     /locus_tag="ENO07_05150"
                     /inference="COORDINATES: protein motif:HMM:PF08308.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HDS01390.1"
                     /translation="MRIRTCILLVLVLAAPLLAQTEGNLEVTSDPPGTTILLDGEFRL
                     AGVTPTVFSQPLNGRYTLKASREGYESYETELLLTGGTPLRVNITLAPKTRFKAFLRS
                     AVIPGWGQFYAGEKARGIFFGLAALGSGLVTLIAEEDFRDKKESYEDLLNEFNEERSI
                     EVRKQMEETINLAREEAYDAETLRNISFGILAAVWTYNLVDAVIFFPNKRYESYAPRV
                     SLNTGDDLSSVGLNLSFKF"
BASE COUNT          785 a          670 c          676 g          667 t
ORIGIN      
        1 gaatttatct gcaatccagc ctgaataatc aagactcagt gaaaagggca atacgacaag
       61 ctgcgttata aaaatcgcaa agaaggtggt gaaaatattt tttaagaaaa atcttatgga
      121 attcaaaaag gccgaaaaga agttctcgtt tttcaggact atatagggga tggtataaac
      181 taatggcgaa aagacgagaa acatgaaaag cttgagcgcc acgatgaaga agaacatcct
      241 cctgggattg ccgataagca tttgcataaa aatttccgga agcagcaatt gcacggcata
      301 taagatagca tagattacag cgctggcaag gaccagccag tgatagctgg aaaaagccct
      361 gctcacagcc tcagaaaatt tgaccttttg agcgcggaag aaaccggcaa aaatgatgaa
      421 tgcggcgccg gtcagtaatg agtccactat aatggaaagg acgaggttca aatgggaatg
      481 caccgagggc gatatgagca ggtgctgggg ataatgtgta aatgcttctc ccccgccaaa
      541 cagggaactg gcaccgatcc aggttacaaa gcccgaccag ggcccggaaa aaggattcag
      601 gatcatataa gcaaaagcga tcgagattag aaagaatatg aagaagggag tccatagccg
      661 aatatagaaa atcgacttca atgcatctat aaatgcggat ataaaattat tgatcttcaa
      721 tatggcagac atgccggcgg tccctcatct acttgggagc aaaaacttcg tttgaaccag
      781 cggatgctcc gaaacggtca tatacataaa ctttatagta atatgtgttt ccgaatatca
      841 ccccctcgtc ggtaaacatg gtcgtgttct ggcggttttc gattataatc agattttccg
      901 gtatattggt tactccactg gatgtagagc ggtaaatcct gtacgactca aagtccgagt
      961 tttcattttg tgtccaggtc agcctgaagg tgactgaatc aacgacctgg attgcgagag
     1021 caaccggatc cggagcaata tcggaagtta tatctataag gtagggcaaa tagaaatcag
     1081 gcgcttcgtt gccggcccga tcggtaaaca ttcccgtaac cgatgagcca gcgacctcgg
     1141 tatttggggg gatcacgtag tcaaatttgt aaataccgtt tccggcaacg gcgtcaccgt
     1201 tggagccatt gtcaaataag tcaagtctgc ccagatcgct gatctcaaca cgggcgctgc
     1261 cccccggctc acctgagtcg agcacgaaat ggatgaggtc tcccggctca aatgacgatg
     1321 ccgggccgct gatgtaaaat gtatctattt ccgcccgcgt atcgagagta atattgtcgc
     1381 tgaatgattc cgcggtctga ttgccgtcag agtccttgaa cctggcgtaa accgtcttct
     1441 caccatcacc atcagtcaat tgccagctaa ccgaattaga ataattgagc catttcgcgc
     1501 cggcaaaatc ctcgctgttg gaaagcatga tatgcgcggt gcgctcgggc gcagtggggt
     1561 tgatgaaaac gtcacggctg ctggtaaact ccgcatcatt atcgatggct atcgaaaaca
     1621 cccccgcgat agccgacacc ggcgcggtgg aactgccctc aatattttga gtcgaaagag
     1681 ccgaaacctg atagatatag cgattgccat tctgcacacc atcatcgaca tagccggtcg
     1741 tcgtgctcga atcatacaga tgcacagtat cctgtacatt actggatcta tatatgaaat
     1801 atttggagat gctggccggg ttttccatat cccagcttaa gtttagaata ttgtcgccca
     1861 cctgaatcct gagattggat ggagccgggg gagcgtcagg aaattcgccc aggtcaaaat
     1921 cggagggtat ttcccgatca catgccagcc agaacccgag agacagtata atcaccgata
     1981 taataatatt ttttgtcatc ttgccacctc agaatttgaa gctcaggttt aatccgacgc
     2041 tggataaatc gtcgccggta ttcaaggata ccctcggcgc gtatgattca tatctcttat
     2101 tcgggaagaa gataaccgca tcgaccagat tataggtcca gaccgctgcc agaataccaa
     2161 aggagatatt tctcaatgtc tccgcatcat aggcctcttc cctggccaga ttgatcgtct
     2221 cctccatctg ctttctaacc tcgatggagc gctcttcgtt gaattcattt agcaggtcct
     2281 cgtaactttc ttttttgtcc cggaaatcct cctcggcaat cagggtcaca aggccggatc
     2341 ccagtgcagc caggccgaag aagataccgc gtgctttttc gccggcatag aattggcccc
     2401 agcctggaat tactgcggac cggagaaaag ccttgaagcg ggttttcggg gcgagtgtta
     2461 tattcacacg gagcggggta cccccggtca atagcaattc ggtttcgtag ctctcgtaac
     2521 cctccctgct ggccttgagc gtatatcggc cgttcagggg ctgcgaaaat acggtcggcg
     2581 taactccagc cagcctgaat tccccatcaa gaagtatagt ggtgccgggc ggatctgacg
     2641 tcacctccag gtttccttca gtctgagcca gaagcggtgc ggccaacacc aaaaccagta
     2701 aaatgcatgt ccttattctc atgttttccc caacttccct gttatcataa atcgtcggac
     2761 aaatatagcc caagctaaag tcataagcaa gtaaaaac
//
LOCUS       DSEB01000284            2234 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1007913, whole genome shotgun sequence.
ACCESSION   DSEB01000284 DSEB01000000
VERSION     DSEB01000284.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1007913"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..939)
                     /locus_tag="ENO07_05155"
     CDS             complement(<1..939)
                     /locus_tag="ENO07_05155"
                     /inference="COORDINATES: protein motif:HMM:PF13365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HDS01391.1"
                     /translation="MPGNRLTDRLIALCIFLGLLIPASAAGQRLKNLENEYASIYETA
                     KHYAVTISVSAVPTARSNYFPPSRRGGYETAIGSGFIYDSLGHIVTSSSVTEQGNLFK
                     ITFADGSSRYADLLGVDLENGVAVLKVRQPPYAAPQFADSDRLVPGHWVGLVGNSFGI
                     FPSFAYGIAAGRNDNGDILVTADLSPGCAGGVVVNSEIKVVGMIAYKLTEPVSLGNLN
                     FAERSGMKEKSLLLTDSEIEFPVGGYSLMIPSNKIAESAEGIISGRIHHGAFLGVIPE
                     DLDIIWAKRVFNINYGVYITQVQDNSPAYRAGIREGD"
     gene            complement(947..1642)
                     /locus_tag="ENO07_05160"
     CDS             complement(947..1642)
                     /locus_tag="ENO07_05160"
                     /inference="COORDINATES: protein motif:HMM:PF13490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01392.1"
                     /translation="MNCRKARCYISAYYDDELSYSTKEELLKHIENCTNCQKEMLFHE
                     QVRAGLKALPREELADDFNDRLFARIYSAPRTETTRISNVPSVLTYRLRSLAPVFAAA
                     GFIVIAAFLAFSQFAIIGGGSDNIADRDRGTINSVNYRPPEMNFTRPTQNISASEMAL
                     NAERLDSLQVATFLKDQKRMIDWMRLNAANSFGGFQDRTHNLDQTVEEKYNESRKYIF
                     PVVTNAGSGRNPY"
     gene            complement(1599..2201)
                     /locus_tag="ENO07_05165"
     CDS             complement(1599..2201)
                     /locus_tag="ENO07_05165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDS01393.1"
                     /translation="MTSREKLTDVELMKRIQKQDMVAFNIFVDRYKNRLFTVINRMLD
                     SKEEAEEIVQETFLRVHQHCHTFDFRFAVSTWVYTIALNLARNELRRKKRIKFFDIED
                     FREKLSAPEKKQLGDPTKVMGMLKKALKKLPDKYKEAFILRDIDQLSYEEIAQILSVP
                     LGTVKSRVNRARNLLRDQIKPKMEEYYELSKGSLLHFGVL"
BASE COUNT          556 a          573 c          556 g          549 t
ORIGIN      
        1 atcaccttca cggataccgg cgcgataggc gggagagttg tcctgcacct gagtaatata
       61 gacgccataa ttaatattga aaacccgttt ggcccagata atatcgagat cttcgggaat
      121 gactccaagg aatgcaccat ggtgtatgcg accggaaatt attccctcgg ccgactcagc
      181 tattttgttg gagggaatca tcaggctgta gcctcccacc gggaattcga tttcagaatc
      241 tgtgaggagc agagatttct ctttcattcc ggatcgctcg gcaaaattta aattaccgag
      301 tgaaacaggc tcagttaatt tataggcgat catccctaca accttgattt cggaattgac
      361 caccacaccg ccggcacatc ccggagaaag gtcggcggtg acgagtatgt cgccattatc
      421 gttgcgcccg gcggcaatgc cataggcaaa cgaagggaaa attccaaatg aatttcctac
      481 cagccccacc caatgccccg gtacaagccg atcagaatcg gcaaactgcg gggcggcata
      541 gggcggctgc ctgactttaa gcacggccac accattctcc agatcgacgc ccaggagatc
      601 ggcataacgc gagctgccgt cagcgaacgt aattttgaaa agattgccct gctcagtcac
      661 cgaggaggag gtgacgatat gccccagact gtcatagata aatccgctgc ctatcgcggt
      721 ttcataccca cccctgcgtg acggaggaaa atagttgctt cgcgccgtgg gcacggcgga
      781 aacagatatg gtcacggcat aatgttttgc ggtttcatag atggaggcgt actcattttc
      841 gaggtttttc agacgctgcc cagcggccga tgcgggtatt aaaagaccca ggaatataca
      901 gagcgcaatt agcctgtctg tcagcctatt cccgggcata atccgcttag tatggattgc
      961 ggccagagcc ggcgtttgtg accacaggaa aaatatactt acgcgactcg ttatacttct
     1021 cttctacagt ctggtccaga ttatgggtcc ggtcctgaaa gccgccaaag ctattggccg
     1081 cattgagacg catccagtca atcatacgct tctggtcctt caggaaagtg gcaacctgaa
     1141 gcgagtccag cctttccgca ttcaaggcca tctcagaggc ggaaatattc tgagtgggcc
     1201 gggtgaaatt catttcgggc ggccgataat tgaccgaatt gattgtgccc ctgtcacggt
     1261 cggcaatatt atcgctgccg ccgcctatga tggcgaactg gctgaaggcc aggaaggccg
     1321 cgatgacgat aaaacctgcg gccgcaaaaa ccggggcaag cgatctcagt cgataggtaa
     1381 gaaccgaggg cacattggat atccttgtcg tctccgtacg aggcgcggag tagatccggg
     1441 caaacaaccg gtcgttgaag tcgtctgcga gctcttccct ggggagagcc ttgaggccgg
     1501 ctcttacctg ctcatggaaa agcatttcct tctggcaatt agtacaattc tcaatgtgct
     1561 ttaacaattc ttccttagta ctgtacgaaa gctcatcatc ataatacgcc gaaatgtaac
     1621 agcgagcctt tcgacagttc ataatactcc tccatcttgg gctttatctg atcccgcagg
     1681 agattccggg cacggttgac ccgggatttg acggttccca gcgggaccga gagaatctgg
     1741 gctatctctt cgtaagagag ctgatctata tcacgtaata tgaatgcttc tttatactta
     1801 tcagggagct tcttgagcgc tttcttgagc atgcccataa ctttcgtggg gtcgccgagc
     1861 tgcttcttct caggtgccga aagcttttcc ctgaaatcct cgatatcgaa aaatttgatg
     1921 cgcttcttac ggcgcaactc gttacgcgcc aggttcaagg caatcgtgta aacccaggtg
     1981 gataccgcga aacggaaatc gaaggtatgg cagtgctgat gcactctcag aaatgtctcc
     2041 tgaacgatct cctccgcctc ctccttggag tcgagcatgc ggtttattac tgtgaaaagt
     2101 cggtttttgt agcggtcaac aaatatatta aaagccacca tgtcctgttt ctggatgcgc
     2161 ttcatcaatt caacgtcagt caatttttca cgcgaagtca taaatatccc ttctgcttta
     2221 tattaaatat acac
//
LOCUS       DSEB01000285            1932 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1010913, whole genome shotgun sequence.
ACCESSION   DSEB01000285 DSEB01000000
VERSION     DSEB01000285.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1010913"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(63..1565)
                     /locus_tag="ENO07_05170"
     CDS             complement(63..1565)
                     /locus_tag="ENO07_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012663137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13/DASS family transporter"
                     /protein_id="HDS01394.1"
                     /translation="MAKKIAIIAGPLLALVIILFFDLDPDNRNVTLTAAVAVWMAVWW
                     ITEAIPIAATALLPVALFPLLGVAEGREIAGKYFNHIIFLFMGGFMIALALQRWNLHK
                     RIALKIMLLIGTSPSRIILGFMAASFFLSMWISNTATVMMMLPIAMAVIARLKDSFEK
                     QGVDKVATSLLLGTAYASSVGGMATLIGTPPNLAFAKIFEETFPMAPEISFAGWIIFS
                     LPISLVFLLFIWAFMSFKARMGKGRKAANRSIFIEEYKKLGPFSYEEKVVMIDFMILA
                     FLWLFRNPIPVGDFVIPGWSTLLPVPEFIDDGTVSISMALILFLIPSRTRPGRMIMDW
                     KTAVSLPWGIVILFGGGFALAYAFKASGLSVWIGSQLGGLAAFHPLLIIAVICLTMTF
                     LTELTSNTATTQTILPIIAGVAIGIGVNPLLLMVPATLSASCAFMLPVATPPNAIVYG
                     GGDFRIATMAKAGIVLNLFGVFLISLAFYFMGRIILGVELGAFPDWAVAP"
     gene            complement(1769..>1932)
                     /locus_tag="ENO07_05175"
     CDS             complement(1769..>1932)
                     /locus_tag="ENO07_05175"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014043074.1"
                     /note="heat shock protein involved in degradation of
                     misfolded proteins; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HDS01395.1"
                     /translation="IVAIGSGGPYALAAARALMKNTRMSAENIARKALEIAAEICIYT
                     NDNITVETL"
BASE COUNT          541 a          518 c          455 g          418 t
ORIGIN      
        1 cttatggatt ggtgggatga gaacattagc tgagctcagg ataagcgcgc gcctgcgcca
       61 tcctaaggtg cgaccgccca atcgggaaag gcacccagct ccacgcccag aattatacgt
      121 cccatgaaat agaaagccag acttatcaga aacacgccga agagattcag cacaattccc
      181 gcctttgcca tagttgcaat cctgaagtcc cctccgccat agactatcgc attgggcgga
      241 gtcgcaacag gaagcataaa tgcgcaggag gccgaaagag tcgcaggaac catcagaagt
      301 aacggattta ctccaattcc gatcgccacc cccgctatga tcggcagaat ggtctgtgtg
      361 gtggcagtat tcgaggtgag ttccgttaaa aaagtcatcg tgagacatat tacggctata
      421 atcaaaagcg gatggaaagc cgccagcccg cccagctggc ttccgatcca gaccgacagc
      481 ccggaggcct taaaggcata ggccagcgca aaaccgcccc cgaataaaat cacgatgccc
      541 catggcagcg agacagcggt tttccaatcc atgatcatgc gccccggccg cgttcttgac
      601 ggtatcaaga ataaaatcag agccatgctg atcgatactg tgccgtcatc tataaattcc
      661 ggtaccggca ggagggtcga ccatccggga atcacaaaat cccctaccgg aatcggattc
      721 cggaacagcc acaaaaaagc aaggatcata aaatcaatca tgaccacttt ttcctcatag
      781 gagaacggtc ccagcttctt gtattcctca atgaaaatgc tcctgttggc cgctttgcgg
      841 cctttcccca tccgggcttt gaaggacata aaggcccaga taaagagaag aaataccaat
      901 gatatcggca gggaaaatat aatccagccc gcaaacgaga tctccggcgc catcggaaat
      961 gtctcctcga atatctttgc aaaggccagg ttggggggtg tgccgatcaa cgttgccatg
     1021 ccgcccacag aagaggcgta tgcagttccc aggagcaggc tggtggcgac cttgtccaca
     1081 ccttgtttct caaagctgtc tttcagcctg gcgataacag ccatcgcaat cggcagcatc
     1141 atcatgacag tagctgtatt ggaaatccac atcgacaaaa aaaatgaagc tgccatgaaa
     1201 ccgaggatga tccttgaggg actggttccg atcaagagca tgattttgag agctatccgt
     1261 ttatgcagat tccagcgttg cagcgccagc gcaatcataa atccgcccat aaataagaaa
     1321 ataatatggt tgaaatactt gccggcaatt tcccgcccct cagcgacgcc cagaagcggg
     1381 aacagcgcca ccggcagaag agctgtggct gcaatcggga tagcctcggt aatccaccac
     1441 acagccatcc atacggcaac cgccgcagtc agggtaacat ttcgattatc aggatcgaga
     1501 tcgaaaaaga ggatgattac cagggccagg agaggtcctg ctataattgc aatctttttg
     1561 gccatacaaa cccgcggcaa aggactaact tatccggtaa tctcccgcat cagattctgt
     1621 aattcatcgg aatcgagaga tttaatcttt tttagaaaat ctgccgactc agtccccttg
     1681 agtacttttg ccaggccctt cttggaatat atataaacct ggcgatcccc ggttacacga
     1741 tagaaatatt tattttcaga catagattct aaagcgtttc aacagttata ttgtcattag
     1801 tgtaaatgca gatttcagct gcaatttcaa gcgccttgcg cgcgatattt tccgcgctca
     1861 tcctggtatt cttcatcagg gctcgtgccg ccgccagagc atagggcccg cccgaaccga
     1921 tagccactat ac
//
LOCUS       DSEB01000286            5847 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000935, whole genome shotgun sequence.
ACCESSION   DSEB01000286 DSEB01000000
VERSION     DSEB01000286.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5847)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5847)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5847
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000935"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..660
                     /locus_tag="ENO07_05180"
     CDS             <1..660
                     /locus_tag="ENO07_05180"
                     /inference="COORDINATES: protein motif:HMM:PF06267.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HDS01396.1"
                     /translation="VRQVAMIDNKGNIATHTGENCIAMAGHRVGPNYSVQANLMEKAT
                     VWDAMAQAFEKTEGELADRMMAALEAAQAEGGDIRGKQSAAMLVVTGEPTGMPWTDRI
                     VDLRVDDSPEPLQELKRLISINRAYDYMNLGDEYFAKGEIEKAKEAYSKSAKLAPGNA
                     EILFWQAATMVSAGEEDTAMTIFKKVFEMDENWRILVPRLVDSGLLPDDPELIERIVA
                     Q"
     gene            1368..2744
                     /locus_tag="ENO07_05185"
     CDS             1368..2744
                     /locus_tag="ENO07_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HDS01397.1"
                     /translation="MPEKAISRIWAEWVHGLKFEDLDDETIYHAKRILYDAMGCALGG
                     HRAEDCEISLAVIEHMGGNPEATLIATGFETNAYMASLANALMVRVMDFNDIYWKEDP
                     SHPSDIIPAALAVGERQGKSGKDLILGVVIGHELEMRLCEFFKPGIRERGWHHASLTQ
                     LVSPLVAGKMLDLDPDQMTNAVGISGCHNFSLGAVAAGKLTMMKNTVDPMATQSGVMA
                     ALLAQKGYIGTELIFEGKEGVCQQMGEGFDYNELTEGLGDSFKIRECYYKAFPTEFLT
                     QSPVTAALKLVKEKDIDWKDVTGVKVHTIARAVDILADPSKYRPTSKETADHSMPYCV
                     GAAIMDRMITPLQFKPEKISNPELLALIQKIEVVADKKLEEEFPVMQPSVVDIKVKSG
                     EEFSQRVDYAKGDPREPMTDDDLRDKFYGLTAEFMKDDQRNKIHEMIFNMENVPTIKE
                     FMKAMIVK"
     gene            2781..3335
                     /locus_tag="ENO07_05190"
     CDS             2781..3335
                     /locus_tag="ENO07_05190"
                     /inference="COORDINATES: protein motif:HMM:PF01613.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDS01398.1"
                     /translation="MKKVDFPIDKRKWHPGLIPGLIVLVSTYSKESIPNIAPISWIQM
                     VSFKPPVLMFSGSRGSRTVNNILETNCFCVNTVDSKMVTKVFNCIKWFGQERIKKCGF
                     NLSDAELIKAPLVDESRAHLKCTLYDTKEVGRAVIVFGEIKAASIWEEIALADTISAY
                     KKLDQVLYMEDGIYALFDRLHRIE"
     gene            3389..3760
                     /locus_tag="ENO07_05195"
     CDS             3389..3760
                     /locus_tag="ENO07_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01399.1"
                     /translation="MLKNLLLVLFMFVLATPLLSARTFDFMKLSDEVSFYFNSNDNFN
                     TKGRTLQIVAINQVKIGTWFQVEFTGDFNWETTIGVNYDYYIEVGVVKKVWRNLSFNY
                     QRIHGSFVDEPINQFGIRLAL"
     gene            complement(3764..4282)
                     /locus_tag="ENO07_05200"
     CDS             complement(3764..4282)
                     /locus_tag="ENO07_05200"
                     /inference="COORDINATES: protein motif:HMM:PF00583.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDS01400.1"
                     /translation="MDLSTQQAKTEHIEDIQKIARTAWGEMCPDALADSDVSQYFNEK
                     YGADDLKEWLIHDGTWFYVLLDSDRVVGFCQLTREQDEKNIAVFEKFLLYPDYQEQKI
                     AYRLFHQVIKAATSHGVTEVVIDIPECSKKWRDVFTHIGVEFETWRKFDKKVGSKEIT
                     FWPGLLTIVPGC"
     gene            complement(4306..5517)
                     /gene="lhgO"
                     /locus_tag="ENO07_05205"
     CDS             complement(4306..5517)
                     /gene="lhgO"
                     /locus_tag="ENO07_05205"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015152327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HDS01401.1"
                     /translation="MKNNNRYDIVIIGGGIIGTATAMAHCKKPGLSLAVLEAEECLAY
                     HQSGHNSGVIHSGIYYKPGSLKARLSVEGREALYEFCKEHNIPHERCGKVIVAVDEEE
                     LKYLDTLLERGRDNGMSEIKKLDADGVRKYEPHVAGIAGIHVPDTGIVDYIKVVEKYA
                     EIIRAAGSDIILNARVSAITRDENGITLSTRKGDFHASCIINCAGLNTDRVARMAGLK
                     PQLQIIPFRGEYYTIKPERRGLVKNLIYPVPDPRFPFLGVHFTRLIDGRVEAGPNAVL
                     ALSRRAYRKTDFSFLEMANLAAYSGFWKMARKYWKTGLEEYLRSWSKPAFVKALQKLL
                     PDLKEDDIIEGGSGIRAQALEPDGTLVDDFRIVEAEDMIHVLNAPSPAATASIRIGEY
                     IAELAERNFKL"
BASE COUNT         1563 a         1403 c         1405 g         1476 t
ORIGIN      
        1 gtgagacagg tggcgatgat tgataacaag ggaaacatcg ctactcatac aggagaaaac
       61 tgtattgcca tggcgggtca tcgcgtcggc ccgaactatt ctgtgcaggc caacctgatg
      121 gagaaggcta ctgtctggga tgctatggcg caggcattcg agaaaaccga gggcgaactg
      181 gcggaccgta tgatggctgc attggaggcg gcgcaggccg agggcggcga tatccgcggt
      241 aagcagtcgg cggccatgct tgttgtgaca ggagagccga caggaatgcc ctggacagat
      301 cggattgtcg atctgcgtgt tgacgactcc ccggagccgc tgcaagaatt aaaacgcctt
      361 ataagtatca accgcgctta tgattacatg aatctcggcg atgagtactt tgcgaagggc
      421 gagattgaaa aagccaaaga agcctactcc aaatcagcca agcttgctcc cggcaatgcc
      481 gagattcttt tctggcaggc cgcaactatg gtctctgccg gtgaagagga tacggctatg
      541 acgattttta agaaagtctt cgagatggat gagaactggc ggattctagt acctcgcctg
      601 gtcgactcag gtcttcttcc cgatgatccg gaattgatag agagaattgt ggcacagtaa
      661 gaaaagacat aaatcagggt gacccaatct tcagggtttg ttgaaaaagt cagcaacaca
      721 gaaataagaa ccgtacacgt cattccgagg gagtcccgcc gaaggcgagg acgaccgtgg
      781 gaatctccgc ggttgcctca agaataatcg cagaaatcgc cacgtccgtc aggagccgga
      841 ttcaaaatcg cttcgctcct cgcgatgacg aatttaggtg ttttcaggtt tatcaacact
      901 cccttcagat tggggagggt gttccggtta aaaatcctaa gctaaaacat ggactattac
      961 ccatatcgta agttttcttt tcatagtaaa catagtagga tggccagcta aacccacatt
     1021 tcatgtgggg tacaaatata agcaaaatag aaataagaac cgataaacgt cattccgagg
     1081 tagtcccgcc gaaggcgagg acgaccgtgg gaatctccgc ggttgcctca agaataatgg
     1141 cggagatcgc ctcgtccgtc aggagccgga ttcaaaatcg cttcgctcct cacgataacg
     1201 aatatcgatg ccttcagggt ttatcaacaa gggctaaagc ttgaggctcc ctggcatctc
     1261 accttatcga aaagcgagcg caaatcccct ccacatatcg cttgccccgg ccggaagaaa
     1321 atatatatta tgccacaaat cgattttcac acaaaaggag ataattaatg ccggaaaaag
     1381 cgatttcaag aatatgggct gaatgggttc atggacttaa attcgaagat ttagatgatg
     1441 aaaccatata tcatgccaaa cgaatccttt atgacgcaat gggatgcgcg ctgggcgggc
     1501 atcgcgccga ggactgcgaa atctccctgg ctgttattga acatatgggc ggcaatcccg
     1561 aagcaaccct gatcgccacc ggtttcgaga ccaacgccta catggcttcg ctggccaatg
     1621 ccctgatggt gcgggtaatg gattttaatg atatatactg gaaagaggat ccctcgcatc
     1681 cctcggatat aatccccgcc gcccttgcag tcggcgaacg ccaggggaaa tccggcaaag
     1741 atcttattct tggtgttgtg atagggcatg agctggagat gcgcctgtgc gaattcttca
     1801 aaccgggaat ccgtgaacgc ggatggcatc acgcctcgct gactcagttg gtatctccgc
     1861 ttgttgccgg aaagatgctc gatctcgacc cggatcaaat gaccaatgct gtcggtatct
     1921 ccggatgtca caatttcagc ctgggtgctg tggcggccgg caagctcaca atgatgaaaa
     1981 atactgtcga tcccatggct acccagtcgg gggttatggc cgcgctgctg gcacaaaaag
     2041 gctatatcgg tactgagttg atttttgagg gcaaagaggg tgtttgccag cagatgggag
     2101 agggtttcga ttataacgag cttacagaag gcttggggga cagtttcaag attagagagt
     2161 gctactacaa agctttcccg accgaatttc taacccagtc gccggtaaca gcggcgctca
     2221 agctggtcaa ggaaaaggat atagactgga aggatgttac cggagtgaaa gttcacacaa
     2281 tcgcgcgagc agtggatatc ctggccgatc cttccaagta tcgtcctacc tctaaggaaa
     2341 cggcagatca ttcgatgcca tattgcgtgg gcgcagctat aatggatcgc atgataactc
     2401 cgctgcagtt caagccggag aaaatctcaa atccggaact cctggcattg atccagaaga
     2461 tcgaggtggt ggctgataaa aagctggaag aggaatttcc ggttatgcag ccctcggtcg
     2521 ttgacataaa agtcaaatcc ggcgaggaat tcagtcagcg tgtggattac gccaagggcg
     2581 acccgcgcga gccgatgact gatgatgatc tcagggataa attctatggc cttactgctg
     2641 aatttatgaa ggatgaccaa cgcaacaaaa tccatgagat gatattcaat atggaaaatg
     2701 tgccgaccat caaggaattc atgaaagcta tgatcgtgaa ataatcataa atttcagata
     2761 tattccgaaa ggggcggatt atgaaaaaag tcgattttcc gattgataag cgcaaatggc
     2821 atcccggcct tattcccggc ttgattgtgc ttgtttccac ctacagcaaa gagagcatac
     2881 ccaatatcgc ccccattagc tggatccaga tggtttcatt caaaccgccg gtcttgatgt
     2941 tttccggatc gagaggcagc cggacagtaa acaatatact tgaaaccaac tgcttctgcg
     3001 tcaacaccgt tgattccaaa atggtcacga aggtttttaa ctgcataaaa tggttcggcc
     3061 aggagcgcat caaaaaatgc ggattcaatc tgagcgatgc cgaacttatc aaggcgcctc
     3121 ttgtcgatga gtcccgcgca catctgaaat gcacccttta tgatacaaaa gaagtaggac
     3181 gcgcagtgat agtctttgga gaaataaaag cggcctcaat ctgggaagaa atagcgctgg
     3241 ccgatacaat tagtgcctac aaaaagctgg atcaggtttt gtacatggaa gacggaatct
     3301 atgccctctt tgatcgtttg catagaattg aataaatctt aaaattatat ctttctgctc
     3361 gcaagtcgtt caaaccggga ggttgaaaat gctgaaaaac cttttattgg tgctgtttat
     3421 gtttgttctc gcaaccccgc ttctctccgc ccgaactttc gactttatga agctctctga
     3481 tgaggtcagc ttctacttta actccaatga taatttcaac actaagggac gcacgctcca
     3541 gattgtggcc ataaatcagg tcaagatcgg tacctggttt caggtggagt ttaccgggga
     3601 tttcaactgg gagacaacca tcggggtcaa ctatgattat tacatagaag tgggagttgt
     3661 aaaaaaagtc tggagaaatc tgagcttcaa ctaccagcgc atccacggct catttgtgga
     3721 tgagccgata aatcagtttg gaatacgtct cgcattatag agatcaacat cccggcacga
     3781 ttgtcaacag ccccggccag aatgtgatct cctttgaacc gaccttcttg tcgaatttgc
     3841 gccaggtctc gaattccacg cctatatgtg tgaaaacgtc gcgccatttt ttcgagcact
     3901 ccggtatatc gatcacaacc tcggtgacac catggctggt agcggccttt atcacctgat
     3961 gaaataatct ataggctatt ttctgttctt gataatccgg atatagcagg aatttctcaa
     4021 agaccgcaat attcttctca tcctgctcac gggtcaactg gcagaacccg accacacggt
     4081 cagagtcaag cagtacataa aaccaggtac cgtcatggat cagccattcc tttagatcat
     4141 ccgcgccgta tttctcattg aaatactgcg aaacatcgct gtcggccaga gcatcaggac
     4201 acatctcccc ccacgcggtt ctggcaatct tctgaatgtc ttcaatatgt tctgtttttg
     4261 cctgttgagt cgataaatcc aaaactaacc tccgcttttc tctattcaaa gtttgaaatt
     4321 tctttccgca agttctgcaa tatattctcc tattctgatt gaggctgtcg ctgccggaga
     4381 gggggcattg aggacatgaa tcatatcctc ggcttccaca atccggaaat catcaacaag
     4441 agtaccgtcc ggttcaagag cctgggctcg aattcccgag ccgccctcaa taatatcatc
     4501 ctccttcaaa tccggcaaca gcttttgcaa tgcctttacg aaagccggct tgctccagga
     4561 acgcaaatat tcttccaggc cagtcttcca atattttctg gccattttcc agaaaccgga
     4621 ataggccgcc aggttggcca tttccagaaa tgaaaaatct gtcttcctgt aagctctgcg
     4681 gctgagcgcc agaacagcat tgggcccggc ctccaccctg ccgtcaatca aacgggtgaa
     4741 atgcactccc agaaacggga aacgcggatc gggcacaggg tagatgagat ttttgaccaa
     4801 cccccgcctt tccggtttga ttgtgtaata ttcacccctg aaaggtataa tctgcagttg
     4861 gggttttagc ccggccatac gggccacacg atctgtgttc agaccggcgc agttaataat
     4921 acaggatgca tggaaatcgc ctttgcgggt tgaaagagta atgccgttct cgtcgcgagt
     4981 aatagccgac accctggcat tcaaaattat atcggatcct gccgcgcgga ttatttccgc
     5041 atatttctcg actactttga tataatctac aataccggtg tcgggtacat ggattccggc
     5101 aatccctgcc acatgaggct catatttacg gacaccatca gcatcgagct ttttgatttc
     5161 actcatgcca ttatccctgc ctctttccag aagggtatcg agatacttca attcctcttc
     5221 atcaacagca acaatgactt taccacatct ctcatgaggt atattatgtt ctttgcagaa
     5281 ttcatataga gcctcgcgtc cctcaaccga taatctggcc ttgagcgatc ctggcttgta
     5341 ataaatacct gagtgtatta cgcccgaatt gtgtccgctt tgatgatagg ccaggcattc
     5401 ctcggcctcc agtactgcca gagaaagccc cggcttcttg cagtgagcca tggcggtggc
     5461 agtgcctata attccgcccc cgataattac aatatcgtat ctattgttat tcttcatccg
     5521 tagactttcc catataacat tgttaatata acagttaaat tgtgcaaaag ttaatataaa
     5581 gtcgttctat ggctttgtcc ggaatggcct gaaaatcact gcctgcgagt tgaaaaaaat
     5641 atgtttttac tggattatat ggaagttata tttataaatt accgtcatgg aattagtcgg
     5701 cagaagaccg gaacgccgga ttttgatata ttttctctcc gccatacttt gcggtgcggt
     5761 tctgctcagc ctgcctgtaa gtgcggcgga ggagcccatc gcttttattg atgccttgtt
     5821 tacctcgacc tcggcggttt gcgtaac
//
LOCUS       DSEB01000287            3817 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002400, whole genome shotgun sequence.
ACCESSION   DSEB01000287 DSEB01000000
VERSION     DSEB01000287.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002400"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            1..258
                     /locus_tag="ENO07_05210"
     CDS             1..258
                     /locus_tag="ENO07_05210"
                     /inference="COORDINATES: protein motif:HMM:PF01075.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01402.1"
                     /translation="MKYYDFLITPDTSLTHLASAFDLPSAVMFSGDERNFGEWGPYNP
                     KIRAVRSPDLKHIEQLEPEALFSAFKGVVAEEKAASHEKAR"
     gene            242..994
                     /locus_tag="ENO07_05215"
     CDS             242..994
                     /locus_tag="ENO07_05215"
                     /inference="COORDINATES: protein motif:HMM:PF00535.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDS01403.1"
                     /translation="MKRRAELSVAMIARDAEGQIGRALDSVIWADEICVADTGSRDRT
                     KEVAARFGARVASVDFSSGFGRAKQAAVRMTTNDWILSLDSDEVVTPELRRSIEQFLL
                     DCDAYAGARVGRITNLCGHWIHHSGWYPEYVLRLFNKKHAGFDDSPLHESIKFNGKIK
                     RLSGCLLHYSYPDLAVYVRKTREYARWGALKKRQLPVFMKLLCMLIKPAAAFIRKFLL
                     QRGFMDGLPGLWIACFTSGGQFLKYYYALRRK"
     gene            975..2063
                     /locus_tag="ENO07_05220"
     CDS             975..2063
                     /locus_tag="ENO07_05220"
                     /inference="COORDINATES: protein motif:HMM:PF13439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDS01404.1"
                     /translation="MPSEENKTKIALCDLGRSFRGGQRQTLNLARTLQYNGHEVLVIC
                     HRNGELLKHCELNGIDVIASKYSILSLLLEGHRIYRELKKIEAQIFHASDSHSHSLGL
                     IVKRFNPSLKMVVTVRTAFDKSTSVSKRLKYNSPRVNAYVAISKAVFDVLLKKGIAPS
                     KIKVIPSSVDYAVFNPGDRVESKVFRIGTAGALEKDKGVDLILLALAKINSELGDYVF
                     KVAGEGPYRESLGLQAGKLGLSDKVRFLGFVNDMPDFYSNLDLFILASKSEGLGSSLL
                     EAGACGAVIAGAEIPGIQEIITDNENGFLFERHELNRLSDIILNLSGDHELGNKIRKE
                     FYNNLRKFDINNITGQYLNLYRKLLENN"
     gene            2065..>3817
                     /locus_tag="ENO07_05225"
     CDS             2065..>3817
                     /locus_tag="ENO07_05225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDS01405.1"
                     /translation="MIGRRPPQENFKYMEVDQFIEEVKKVNASVRTGLLRKAFEYADR
                     AHKDQMRASGEKYFTHSINVALILAAQKLDTTTIAAGLLHDVVEDTDVTVKDLRRDFG
                     DEIAELVDGVSRISTYQAKTSIETKAEYFRKMLLSMAKDIRVILIKLADRLHNMRTLE
                     YLSEKRQIKNAKETYEVFAPIAHRLGMALIKRELEDLSLKYLHPEEYQYIKNRLDNTL
                     QARESYVKDIIGPLQKELKANNVAAEVYGRAKSIDSIHRKMTRKERSFEEIYDLFAIR
                     IIVDSVAACYHALGVVHMLYVPVGGFDDYIAKPKQNGYQSLHTNVIGPDGHVLEFQIR
                     TREMHMRAEYGIASHWLYKEGARGIGEADKRLNWLREVLDWQKDMTNPAEFLEYLKID
                     LYHDEVFVFTPRGELKHLPSGSTALDFAFAVHTEVGLHCAGARINGKLVPLNTELRSG
                     DEVEVMTATNKQPSRDWLKMVNTSGARSKIRKFIKQQDFEKNRQMGKEMLERHLRKMR
                     LPYPSNKEILECAESFSYKNVKSFLAALGQGDVSVISAVNRLYPQALEESKESIEDII
                     TDRTRFRGGIKIDDIKSL"
BASE COUNT          988 a          911 c         1053 g          865 t
ORIGIN      
        1 atgaaatatt atgactttct tataacgccg gatacctccc tgacacatct ggccagtgca
       61 ttcgacctgc catcggcagt catgttctca ggggatgaaa gaaatttcgg agaatggggg
      121 ccatacaatc cgaagatcag ggcagtccgc tccccggacc tgaagcatat cgaacagctc
      181 gagcccgagg cccttttcag tgcgtttaag ggggttgttg ccgaggaaaa ggcggccagt
      241 catgaaaagg cgcgctgagt tatcggtggc catgattgcc cgcgatgcgg aaggtcaaat
      301 cgggagggct ctggattcgg ttatctgggc ggatgagatc tgcgtggccg acaccggctc
      361 ccgggataga acaaaagagg ttgcggcgcg attcggcgcc cgcgtggcaa gtgtggattt
      421 ctcttccgga ttcgggaggg ccaaacaggc ggctgtgcgg atgaccacca atgattggat
      481 tttatcgctc gactctgatg aggtggtcac tccggaactg cgcagaagca tcgaacaatt
      541 cctgctggat tgtgatgctt acgccggcgc cagagtgggc cggattacga atctgtgcgg
      601 gcactggatt catcattccg gatggtatcc cgaatatgtg ctgcggctgt tcaataagaa
      661 gcatgcgggg tttgatgaca gcccgctgca tgaatcgata aaattcaatg gaaagattaa
      721 aaggctttcg ggctgcctgc tgcattacag ctatcccgat ctggcggtct atgtgcgcaa
      781 gaccagggaa tatgcccgct ggggagcatt gaagaagaga caacttcccg tattcatgaa
      841 gctgctctgc atgctgatca agcctgccgc ggcattcata cggaagtttc tgctgcagag
      901 aggatttatg gacggccttc cgggtttgtg gattgcctgt ttcacgtccg ggggccagtt
      961 cctgaaatat tattatgccc tcagaagaaa ataaaactaa gatcgcatta tgtgatctgg
     1021 gacgcagttt caggggggga cagcgccaga ccctgaacct cgcccgcacg ctgcagtata
     1081 acggccatga ggtgctggtc atctgccaca ggaacggcga actgcttaaa cactgcgaac
     1141 tcaacggtat cgatgtgatt gcctcgaaat atagcatcct cagcctgctg ctggaaggac
     1201 atagaatcta ccgcgaattg aaaaaaatag aagcacaaat atttcatgcc tccgattcac
     1261 acagccattc gctgggactg atcgtaaaga gattcaatcc ctcgctgaaa atggtggtga
     1321 cggtccgtac cgcttttgat aaatccactt cggtgtcaaa acggctgaaa tataattcgc
     1381 ccagagtaaa cgcctatgtt gccatcagca aagctgtgtt cgatgtttta ttgaaaaagg
     1441 gaattgcccc ctcgaagatc aaggtcatac ccagttcagt tgattatgct gtattcaatc
     1501 cgggcgaccg ggtcgaatca aaggtcttca gaatcggcac tgccggagct ttggagaaag
     1561 acaagggagt ggatttaatc ctgctggcac ttgctaagat taacagtgag cttggagatt
     1621 atgtgttcaa ggtcgccggc gagggaccct atcgggaatc gcttggactt caggccggaa
     1681 aattgggttt aagcgataag gttcggtttc tggggtttgt caatgatatg ccggatttct
     1741 atagcaacct ggacctgttt atacttgcct cgaagtcgga aggactggga tcatcgctgc
     1801 ttgaagcagg cgcctgcggg gcggtgatag ccggcgccga aatacccggt attcaggaga
     1861 tcattacgga caatgaaaac ggcttcctgt ttgaaagaca tgagctgaac agactatcag
     1921 atataattct caatctgtca ggggaccatg agctggggaa caaaatcaga aaagaattct
     1981 ataataatct gcgtaagttc gatataaata atataaccgg gcagtattta aacctttaca
     2041 gaaagttgct ggagaataat tgatatgatc ggccgcaggc cgccgcagga aaatttcaaa
     2101 tatatggaag ttgaccagtt catcgaggag gtcaaaaaag taaatgcctc tgtccgcacc
     2161 ggtctgctgc gcaaggcatt cgagtacgcc gaccgggccc acaaggatca gatgcgcgcc
     2221 tcgggcgaga aatatttcac gcattcgatc aatgtagcgc tgattcttgc cgctcaaaag
     2281 ctggatacca ccacgattgc agccggcctt ctgcatgatg tggtcgagga taccgatgtg
     2341 acggtgaagg atctgcggcg ggatttcggg gatgagatcg ctgagcttgt ggacggggtc
     2401 agccggattt cgacctacca ggccaagacc tcgatcgaga ccaaggcgga gtattttcga
     2461 aagatgctcc tgtccatggc caaggatatt cgtgtaattc taatcaagct ggccgatcgg
     2521 ctgcataata tgcgcacgct cgagtacctt tccgagaaac gccagataaa aaatgctaag
     2581 gaaacctatg aggtgtttgc gccgatagcg catcgtctcg gaatggccct gattaagagg
     2641 gaacttgagg atctttcgct caaatacctg catccggaag agtaccaata catcaagaac
     2701 cgcctcgaca acacattgca ggcccgggag tcgtatgtca aagatattat cgggcccctg
     2761 cagaaggaac tcaaggccaa caacgtggca gccgaagttt acggccgcgc caaaagtatc
     2821 gactcgatcc atcgcaagat gacccgcaaa gaaagatcgt tcgaggaaat ttacgacctg
     2881 tttgccatac gaattatcgt cgattcggtt gcggcctgct atcatgctct gggagttgtg
     2941 catatgctct atgtaccggt gggcggcttt gacgattata tcgccaaacc aaagcagaat
     3001 ggctaccagt ccctccatac caatgtgatc ggtccggatg ggcatgtact cgaattccag
     3061 atcaggacgc gtgagatgca tatgcgcgcc gaatatggaa tcgcctcgca ctggctttat
     3121 aaagagggag ccaggggtat cggcgaggcc gataagcgcc tgaactggct gagggaagtg
     3181 ctggactggc agaaggacat gaccaatccg gccgagttcc tggaatatct caaaattgac
     3241 ctgtatcatg atgaggtgtt tgtcttcacc cccaggggcg aattgaagca tcttccttcc
     3301 ggttcgactg cactcgattt cgcatttgct gtgcataccg aggtgggcct gcattgtgcg
     3361 ggagccagga tcaacggcaa gctcgtgccc ctcaataccg agctcaggag cggggacgag
     3421 gtcgaggtca tgaccgccac caataagcag cccagccgcg actggctcaa gatggtgaac
     3481 acctctggcg cccgttcaaa aatcagaaaa ttcatcaagc agcaggactt cgaaaaaaat
     3541 cgtcagatgg gcaaggagat gctggagaga catctgcgca agatgcgtct gccatatccc
     3601 tctaataagg agattctgga gtgcgccgaa tctttctcat acaaaaatgt caagagcttc
     3661 ctggccgctc tcgggcaggg ggatgtttcc gtgatctcgg ccgttaaccg gctttatcct
     3721 caggctctgg aagagtccaa agaatcgatc gaggatataa tcaccgaccg cacccgtttc
     3781 aggggcggga tcaaaatcga cgatataaaa tcactgc
//
LOCUS       DSEB01000288            5846 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000937, whole genome shotgun sequence.
ACCESSION   DSEB01000288 DSEB01000000
VERSION     DSEB01000288.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5846)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000937"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..360
                     /gene="rplK"
                     /locus_tag="ENO07_05230"
     CDS             <1..360
                     /gene="rplK"
                     /locus_tag="ENO07_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015752502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDS01406.1"
                     /translation="PVGPALGQHGVNIMEFCKAFNARTQKQAGNIIPVEITVYQDKSF
                     TFITKTPPAADLLRKAAKLKKASAEPNRDKVGKVTLKQIKEIAELKMPDLNAADVESA
                     MKMIAGTARSMGIEVES"
     gene            376..1080
                     /locus_tag="ENO07_05235"
     CDS             376..1080
                     /locus_tag="ENO07_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001255973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDS01407.1"
                     /translation="MSTGKKYSEFRKKVNSRTAYDLDDAVRLLKENSFAKFDETVELA
                     VRLGVDPKKADQMIRGTVVLPHGTGKKVRVLVFTKGEKVTEAEEAGADYVGLEEYVDK
                     VQGGWFDFDVVVASPDVMGMVGKLGKALGPRGLMPNPKSGTVTMDIGKAVSDIKKGKI
                     AYRVDKVGNVHVGIGKVSFSESQIKENLLAFMDAIVRARPAAAKGQYLRNVSLSSTMG
                     VGIKLNPSGLVAELKS"
     gene            1101..1622
                     /locus_tag="ENO07_05240"
     CDS             1101..1622
                     /locus_tag="ENO07_05240"
                     /inference="COORDINATES: protein motif:HMM:PF00466.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDS01408.1"
                     /translation="MMKRPEKEELVEELGSLFEESSNIFVTDYLGLNVAQITEFRKML
                     REGNIKYRVAKNTLLKIAAKNKGHEEMVQYFEGPTAVAFGVDDPTVPAKTINDFLKKN
                     GKPEVKAFWADGVLYQSERLKELAELPTKDELIARIVGTISAPLTNLVGTLDGLIREF
                     CVTIDQIAKQKES"
     gene            1723..2106
                     /locus_tag="ENO07_05245"
     CDS             1723..2106
                     /locus_tag="ENO07_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDS01409.1"
                     /translation="MSEVIDQIIESIDKLTVMELAELSKALQEKYGVSAAMPVATSVA
                     PGAAAGAGEAEEEKTEFDVVLDAIGDKKIQVIKVVRELTSLGLKEAKELVDGAPNKVK
                     EGVTKEEAEAAKAKLEEVGATIKIQ"
     gene            2333..>5846
                     /gene="rpoB"
                     /locus_tag="ENO07_05250"
     CDS             2333..>5846
                     /gene="rpoB"
                     /locus_tag="ENO07_05250"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDS01410.1"
                     /translation="MAKVTTVDRMSFAKLSSPAEMPNLLEVQLASYEAFIQADMAPMK
                     RKNQGLQKIFNEIFPVTDVHENYTLEFVKYSMGTPRYTIRECQERNMTYAAPLKATLR
                     LVAREQDEKTSEVKDIIEQDVFLGELPLVTPGGTFIINGAERVVVNQLHRSPGVSFDD
                     DIHPNGKRLFFARIIPFRGSWVEFNIDINDIMYVHIDSRRKLPMTTFLRALGFSSNLD
                     LIQQFFEVTDFKLTARSAEKLVGKTAAQDIADPETGEVLVDVGNSFTGEQVEKLKEAK
                     IKDFKIVDLELSQEEKVVFNTFKKDNTTNAEEALFKLYSLIRPGDAPTLELAQNLVES
                     MFFSEKRYDLGEVGRYMVNQRLGLDVPLEQTVLDRRDFIAIIKYLINLRNGHGYIDDI
                     DHLGNRRARTVGELLANLFSVGLSRVARTIRERMSLKDAENITPHDLVNARTVSAVID
                     TFFGSSQLSQFMDQTNPLSELTHKRRLSALGPGGLTRERAGFEVRDVHHTHYGRMCPI
                     ETPEGPNIGLIASLATYARINKYGFLETPYRHVKKGKVTDEIVYLTADKEDNYYIAQA
                     NEPLDDKGNFVNNQITVRFRSDYPIVDPEKIDYMDVSPRQLVSIAASLIPFLENDDAN
                     RALMGSNMQRQAVPLLKTQPPLVGTGMEEKAAHDSMVALVSEVEGEVISVDANEIVIK
                     PSGKVKGDLLGYREEVVYDLIKYKRSNQDTCINYRPCVDVGDKVNVGTLLADGPASHG
                     GELALGYNVLVAFMPWRGYNFEDAIIISERMVKEDIFTSIHIEEFEIQVRDTKRGSEE
                     ITREIPNVSEDAIKHLDESGVVVIGSEVEAGDILVGKVTPKGETELSPEERLLRAIFG
                     EKAGDVRDASLKAPPGLKGIVIDTQVFSRKERTEEAKKREAAEKAKVKKQYNELIDNI
                     KRLTKARLTELLDGYTSNTIRDRDEGTVVIRAGHKYKEDSLDGIDFENVIAEDGWSQE
                     NRVNKKVDELLEEATKILAEKRGELEIEIEKITRGAELPPGVKQLAKIKVAVRRKLSV
                     GDKMAGRHGNKGVISKIVPEEDMPYLEDGTPVDIILNPLGVPSRMNVGQILETHLGWA
                     AQKMNQYYATPVFDGASIDEIKAELKKAGLPESGQVQIYDGRSGELFDNPVTIGYIYM
                     MKLVHLVDDKIHARSIGPYSLVTQQ"
BASE COUNT         1556 a         1307 c         1668 g         1315 t
ORIGIN      
        1 ccggttggtc ccgcactggg acagcatggc gtcaatatta tggagttttg caaggccttc
       61 aatgcccgca cgcagaagca ggccggaaat attattccgg tagaaattac tgtctaccag
      121 gataagtcgt tcacatttat taccaaaact ccgcctgccg cggatcttct gaggaaagcg
      181 gccaagctca aaaaggcctc ggcggagccg aatcgtgaca aggtcggcaa agtaacattg
      241 aaacagataa aagaaatcgc tgagttgaag atgcccgatc tgaatgccgc ggatgtagag
      301 agcgcaatga agatgatcgc cggcacagcc cgcagtatgg gaattgaagt ggaaagttaa
      361 ggagtcgaag tcaagatgag taccggaaaa aaatacagtg agttcaggaa aaaggtcaac
      421 agtcgtactg cttacgatct ggatgacgcg gtcaggcttt tgaaggaaaa ttcgtttgca
      481 aaatttgatg agaccgtgga acttgcggta cgcttaggtg ttgaccctaa gaaagctgac
      541 cagatgatca ggggcacagt tgtgctgcct catggtaccg gcaagaaggt ccgggtactg
      601 gttttcacca aaggtgagaa ggtaaccgag gcggaggaag ccggtgctga ttatgtcggc
      661 ctggaagaat atgtggacaa ggtgcagggc ggctggtttg atttcgatgt ggtggtggcc
      721 agtcccgacg ttatgggtat ggtcggtaag ctcggtaagg cgctgggccc gcgcggcctg
      781 atgccaaatc cgaagtccgg cacggttacc atggatatcg gcaaggctgt gagtgacatc
      841 aagaaaggta aaatcgctta tcgtgttgat aaagtcggca atgtgcatgt gggaatcggc
      901 aaggtctctt tcagtgaaag tcagatcaaa gagaaccttc tggcatttat ggatgccata
      961 gtcagggccc gtcccgcagc ggccaaggga cagtatttga gaaatgtctc gctttcttcc
     1021 accatgggag ttggcataaa attgaatccg tccggtcttg tggccgagct taaatcttaa
     1081 tctgactgga gatagctatt atgatgaagc gtccggaaaa agaagaattg gtcgaagagc
     1141 ttggcagtct ctttgaggaa tcgagcaata tattcgtaac cgattatctt ggattgaatg
     1201 tggcgcagat taccgagttc aggaagatgc tgcgcgaggg aaacattaaa tatcgggtcg
     1261 ccaagaatac gctcctgaag atcgcggcaa agaataaggg gcacgaagaa atggtacagt
     1321 atttcgaggg cccgacggcg gtggcgttcg gagtcgatga tcccactgtt ccggccaaga
     1381 ccataaatga tttcttgaag aagaacggca agcctgaagt caaggctttc tgggccgatg
     1441 gtgttcttta ccagagcgag cgattgaagg agctggccga actgcccacg aaggatgaat
     1501 tgatcgccag gatagtaggt acaatcagcg cgccactgac aaatctggtg ggaacccttg
     1561 atggtttaat aagagaattt tgtgtaacaa tagatcaaat cgcgaaacaa aaagaaagtt
     1621 aattgctgct tctaagccta gatgaattac gcatggctgg atgtgggccg ctgaggtccg
     1681 aggcaacttc cggtcagcaa tgtaacgatt agggagattg aaatgagtga agttattgat
     1741 cagataattg agtcgattga caagctaact gtcatggaac tggccgaact ttccaaggca
     1801 ctgcaagaga agtatggtgt ctcggctgcc atgccggttg cgacctctgt cgctccgggc
     1861 gctgccgcgg gtgccggcga ggctgaagag gaaaaaaccg aatttgatgt tgtcctggat
     1921 gctatcggag acaagaagat tcaggtcatc aaagtagtgc gcgagcttac cagcctgggt
     1981 ctgaaagagg ccaaggagct ggttgacggc gctcccaaca aagtgaaaga gggagtgacc
     2041 aaagaagaag ccgaagcggc caaggcgaaa ctggaagaag tcggcgctac cattaaaatt
     2101 caatagcatt tgccccggcc atacaccggg gtaaatgcca aaaaaatggc ggttatgaag
     2161 ggtggaatgg cggttggaaa tctgttccca agtttcagta tcacccagat cttacccggg
     2221 gtattcagga acatgtgtgc ctgaaatgcc gttagggtga tggccataat gtttatataa
     2281 attgatacag ggctctcggg ctctttaatg tttttaaagg agtgtggttc ctttggctaa
     2341 agtaacaacg gtagaccgta tgtcttttgc caagctttcc tcgccggcgg aaatgcccaa
     2401 tctgctggag gtgcagcttg catcctatga ggcctttatc caggctgaca tggccccgat
     2461 gaagcgcaag aatcagggtt tgcaaaagat ctttaatgaa atttttcccg ttaccgacgt
     2521 tcacgagaat tataccctgg aatttgtgaa gtacagcatg ggaacgcccc ggtataccat
     2581 cagagaatgt caggagcgca acatgactta tgccgctccg ctgaaagcga ctctgaggct
     2641 ggtggccagg gaacaggatg agaagaccag cgaagtaaag gatattattg agcaggatgt
     2701 tttcctggga gagctacccc tggttacacc gggtggaacg ttcatcatca atggcgccga
     2761 gagggttgtg gtcaaccagc tgcaccgttc gcccggcgta tcgttcgatg atgacattca
     2821 tcccaacggc aaaaggttgt ttttcgccag gattattccc ttccggggat cctgggtgga
     2881 attcaatatc gatatcaacg atataatgta tgtgcatatc gattcccgca ggaagcttcc
     2941 catgaccacc ttcctgagag cgctgggatt ttcatcaaat ctggatttaa tccagcagtt
     3001 ctttgaagtt acggatttca aactgaccgc caggagcgca gagaaactcg tcggaaagac
     3061 tgccgctcag gatatcgccg acccggagac cggggaggtc ctggttgatg tcggcaattc
     3121 cttcaccggg gagcaggttg agaagctcaa agaagccaag ataaaagatt tcaagatcgt
     3181 cgatctggaa ctctctcagg aagagaaggt tgtcttcaat actttcaaga aggacaacac
     3241 tacgaatgcc gaggaagcgc tcttcaagct atactccctg atcaggcctg gtgatgctcc
     3301 cactttggag ctggcccaga atctggtgga aagcatgttt ttctccgaaa aacgctatga
     3361 tctgggagag gtcggacgct atatggttaa ccagaggctt ggtctcgatg tgcccctgga
     3421 acagaccgtc ctcgaccgcc gggatttcat cgcaataatt aaatacctga tcaacctccg
     3481 taacggacac ggttatatcg acgatatcga ccatctcggc aaccgccgcg cccgtactgt
     3541 cggcgagctg cttgccaacc tgttctcggt cggtttatcg cgcgtggccc gtacgatcag
     3601 ggagcggatg agcctgaagg atgcggaaaa cattactccc catgatctgg tcaatgccag
     3661 gactgtctcg gctgtaattg ataccttctt cggctcctcg cagctttcgc agtttatgga
     3721 tcagaccaat ccgctctcgg aattgactca caagagaagg ctgtcggccc tgggtccggg
     3781 cggattgacc agggagagag cgggatttga ggtgcgggat gtgcaccata cgcattacgg
     3841 ccgcatgtgt ccgatcgaga ctcctgaggg cccaaatatc ggtcttatcg catccctggc
     3901 gacctatgcc agaattaaca aatatggatt tttggaaact ccttaccggc atgtgaaaaa
     3961 aggcaaggtc acggacgaga tcgtctatct gaccgccgac aaagaagaca attattatat
     4021 cgcgcaggcc aacgagcctc tggatgacaa gggtaatttt gtaaataatc agattacggt
     4081 acgtttccgc agcgactatc cgatagtcga tccggaaaag atcgattata tggatgtttc
     4141 tccccgtcag ctggtcagca tcgctgcctc cctgatacca ttcttggaga atgacgacgc
     4201 caaccgtgcc ttgatgggtt ccaacatgca gaggcaggcg gttccgctct tgaagactca
     4261 gcctcctctg gtgggaacgg gtatggaaga aaaagccgcc cacgattcca tggtggcatt
     4321 ggtctcagag gtggagggcg aggttatttc agttgatgca aacgagatag tcatcaaacc
     4381 ctccgggaaa gtcaaagggg atcttctggg atatcgagaa gaagttgtct atgatctgat
     4441 aaagtataag cgttcgaacc aggatacctg tatcaactac cgtccctgcg tggatgtggg
     4501 cgacaaagtt aatgtgggaa cgctgctcgc cgacggcccg gccagtcatg gaggcgagct
     4561 cgccctgggc tataatgttc tggtggcgtt catgccctgg agaggctaca actttgagga
     4621 cgccattatt atctccgaga gaatggtcaa agaggatatc ttcacctcga tccacatcga
     4681 ggaatttgag attcaggtta gagataccaa gcgcggctcg gaagagatta ccagagagat
     4741 tccgaacgtc tccgaggacg ccatcaagca tctggatgag tccggtgtgg tggtgatcgg
     4801 ttcggaagtt gaagcgggtg atatcctggt tgggaaagtc actcccaagg gcgagacaga
     4861 gctttcgccg gaggagagac ttctgcgggc gatctttggc gagaaggccg gcgacgttcg
     4921 tgatgcctcg ctcaaggctc ctccgggatt gaaaggtatc gtgatcgata cgcaggtgtt
     4981 ttcgcgcaag gaaagaactg aagaagccaa gaagcgcgaa gccgctgaaa aggccaaagt
     5041 caagaagcag tacaatgaac ttatagataa tatcaagagg ctcacaaagg cccgtctgac
     5101 cgaacttctg gatggctata catcgaatac gattcgggat cgtgatgaag gtacggttgt
     5161 cataagagcg ggccataaat ataaagaaga cagcttggat ggaatcgatt ttgagaacgt
     5221 tatcgctgag gacggctggt cccaggagaa cagggtaaat aagaaagtcg atgaactcct
     5281 ggaagaggcg accaaaattc tggccgagaa gcggggcgag ctcgaaatag agatcgaaaa
     5341 aatcactcgc ggtgccgagc tccctccggg ggtcaagcag cttgccaaaa ttaaggtggc
     5401 tgtcaggcgc aagctctcgg ttggcgacaa gatggccggc cgccatggaa acaagggcgt
     5461 gatttcgaag attgttccgg aagaggatat gccctatctc gaggatggaa cgccggtaga
     5521 tattattctc aatcctctgg gcgtgccgtc acgtatgaac gtcggacaga ttctggagac
     5581 tcatttgggc tgggcagccc aaaaaatgaa ccagtattat gccacccctg tcttcgacgg
     5641 ggcctcgatc gacgagatta aggcagaatt gaagaaggcc ggtttgcccg aaagcggtca
     5701 ggtgcagatt tacgacggcc gttcgggcga gctcttcgat aacccggtta caatcggata
     5761 tatatatatg atgaagctgg tacatcttgt ggacgacaag attcatgccc gttccatagg
     5821 accctattcg ctggtcacgc agcagc
//
LOCUS       DSEB01000289            2213 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1008085, whole genome shotgun sequence.
ACCESSION   DSEB01000289 DSEB01000000
VERSION     DSEB01000289.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1008085"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(162..500)
                     /locus_tag="ENO07_05255"
     CDS             complement(162..500)
                     /locus_tag="ENO07_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HDS01411.1"
                     /translation="MKTFVLMTKLAPQSASLIEVGHKHTDRAKCNQEWLDQINEQCPE
                     VEFLAHYALMGTWDFMDIYQAPDERTAAKVSLITREYGACQVESWLAIPYDQIAEITK
                     EIKRPEKAPQ"
     gene            complement(684..1244)
                     /locus_tag="ENO07_05260"
     CDS             complement(684..1244)
                     /locus_tag="ENO07_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01412.1"
                     /translation="MKRVALLIVSAILILALPLAAQDDGRPSYAVDRGSILAGGSAGF
                     VSVGGDARADERISIFTFNPAVLYFAIPNVGVGGQLMFKRESTEGDTKTFYGAGPTAA
                     YFFGGEKSVVFPFLSMSFIYGKKTDEYTETVLRFTGGTTYMLARNVGMTGEIFYLIEN
                     FDMESSDVSVSGNTFGVAFGLSAFIF"
     gene            complement(1320..>2213)
                     /locus_tag="ENO07_05265"
     CDS             complement(1320..>2213)
                     /locus_tag="ENO07_05265"
                     /inference="COORDINATES: protein motif:HMM:PF01916.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HDS01413.1"
                     /translation="LFGYAGSLSTTGQWKIINWLLKQGFIDILVPTGANISEDIVESM
                     GGAYLQGSHVADNEKLFHEGYNRYYDVYGSEKEYLKMTELIADFIMTLDENHKYSSRE
                     FLGLFGKWLKKKSIRSIVTAAYEANVPVFCPAIIDSPYGDAALIAKSRGFELTLDGVK
                     DYIEFMQLGEKVKSTGVVYIGGGVPKDFIQLFAVTGDLLYKDREVPGRSGGHKRQGTD
                     ETYYPHKYAIQVTADAPQWGGLSGCTFEEAVTWGKETNKGEYVQCYCDSTIALPIISH
                     ALAERIKSKRSGKRLSRNFNG"
BASE COUNT          562 a          598 c          484 g          569 t
ORIGIN      
        1 ctattacata ttgcagaaag ctctcgctga cagccataga aactcaacta ctattttcct
       61 taacttatac tcttagtcat tcttaatcgt gttcccgaat ataaaaaaag aaccgcggaa
      121 aatcaaatcc gcggctcatt ccaaaaaacc aaacttcgca cttactgggg ggctttctca
      181 ggccttttaa tttccttggt aatctccgcg atctgatcat agggaatcgc cagccagctt
      241 tccacctggc aggcgccata ttcccttgta atcagcgata ctttggcggc ggtacgttca
      301 tcgggagcct gataaatatc catgaaatcc catgtcccca taagggcata atgcgcaaga
      361 aattccactt ccggacactg ctcgttgatc tgatccagcc attcctggtt gcatttggcc
      421 cgatcggtgt gcttatgacc cacctcaatc aggctcgcgc tctgtggtgc aagcttggtc
      481 atcagaacaa aggtcttcat aataacctcc tgttcttact ataaatatac catctaatcc
      541 ttaatttgaa acaaaaattc ggaattaatg ctgattttgt ttctttgtgc agatacgaga
      601 tgtgtaagta atctattacg cttattgaac ttatgaagac cgggagctta tttttgaaaa
      661 tggattggcc gggataaatc ggatcaaaaa ataaaggcgc tcaggccaaa ggcaaccccg
      721 aaggtattgc cggaaaccga aacatccgag ctctccatat cgaaattttc gatcagatag
      781 aatatctcgc cggtcatacc cacatttctg gccagcatat aggtcgtacc cccggtgaag
      841 cgcagaaccg tttcagtgta ctcatcggtt ttcttgccat aaatgaagct catcgataga
      901 aatggaaaga ccacactttt ctcgcccccg aagaaatatg ccgccgtggg cccggcgccg
      961 taaaatgttt tcgtatcccc ctcggtcgat tccctcttaa acataagctg tccgcccacg
     1021 cccacattgg gaatggcgaa atacagaaca gccggattaa atgtgaagat gcttattctc
     1081 tcatccgcgc gtgcatcccc gcccacactt acaaacccgg cgcttccgcc cgcaagaata
     1141 ctgcccctgt caactgcata gctcggcctg ccgtcatcct gtgcggcgag aggcaaggcc
     1201 aatatcaaaa ttgcggatac tatcaacaga gcgactcttt tcattatccc tcccaattca
     1261 atttgcatta ttctccaaaa tgtaccgcgc cgcctgattt gtcaatttaa tataaacagc
     1321 taaccattga aattccgcga aagccttttg ccggagcgtt tgctcttgat ccgttccgcc
     1381 agggcatgcg aaattatcgg gagcgcaatg gtcgaatcgc agtagcactg cacatactcg
     1441 cccttgttgg tctccttgcc ccaggtcaca gcctcctcga atgtgcagcc ggaaagcccg
     1501 ccccactgag gagcatcagc ggtaacctgg atcgcatatt tgtgcgggta ataggtttca
     1561 tcggtaccct gtcgtttatg cccgccggaa cggccgggaa cctcgcggtc cttgtagaga
     1621 aggtcgccgg ttaccgcgaa aagctggatg aagtccttgg gaactcctcc gccgatatag
     1681 accacgccag tgctcttgac tttctctcca agctgcatga actcgatata gtccttgacc
     1741 ccgtcaagtg tgagttcgaa tccccggctt ttggcgatca gggcggcgtc gccataaggg
     1801 ctgtcgataa tcgccggaca gaaaaccggc acattggcct catatgcagc ggtcacaatc
     1861 gagcggatgc ttttcttctt gagccatttg ccgaataatc ccagaaattc gcgcgaggag
     1921 tatttgtgat tttcgtcaag cgtcatgata aaatctgcga tcaactcggt catcttcaaa
     1981 tattcttttt ccgaaccgta aacatcatag tagcggttat aaccctcgtg gaaaagcttc
     2041 tcgttgtcgg ccacatgcga gccctgcagg taagcccctc ccatagattc gactatgtct
     2101 tccgagatat tggcgccggt aggaaccaat atatcgatga agccctgttt caggagccag
     2161 ttgattatct tccactgccc ggtggtggac aatgatccgg catagccgaa aag
//
LOCUS       DSEB01000290            3061 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003942, whole genome shotgun sequence.
ACCESSION   DSEB01000290 DSEB01000000
VERSION     DSEB01000290.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003942"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            437..>3061
                     /locus_tag="ENO07_05270"
     CDS             437..>3061
                     /locus_tag="ENO07_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01414.1"
                     /translation="MLRGNKLLLALLLPVFVLAFCFAAFALDEDDFIIIDGEHFTPEQ
                     WAAKQEAMADMKVMKTDVQKPIISQAPQDGIDVNDETLRFNPRQHEIVNKAAPATNLL
                     FEDFEGGVVPPTGWTLDAQNTSYTWKLATYDPYNGSNYTNCEYDPALTPQDEWLISPV
                     VDLTTSGSTWQLEFHWYMSYYWGVSPYDNYDLEVWISTDGGTTFSTMLWTEPTTTFTN
                     WTWYDVIIDLTPYLTETNVAFGFRYVGSDGAEGAFDYISVNDTPPPTGRCCYGDPNTP
                     SCADESQAACQARPDWLSWDEGLDCTNNPCPIVGQGDDCTNPITATFPADFAGTQYLN
                     NNYTCGRIDDYDATACLGYYDGGEDIIYEFTVTQDIYINITLDPKGTTWNGFALGTDC
                     PPNSCVAVSTSSAGSPHGVSNVILTAGTYYVMVDTWPSPDCIPNFDLIIDYLGDPPPT
                     PDNDECANAELVAGPFPQTVTGSTVNSTIDCPGLLDWYAVWYYFENPYSCANLVIDWC
                     GTTPDLAVVGVVVMDDCACDDYILHNNTTWDECGDGNPTTYWNGLAAGTYYYAAFSGP
                     NSSGTGQTQDHTITFDLQECPPAPPNDNCAGAEVITLGTPVSGTTAGATPDCPSLFTD
                     WYGVWYQFTLSSSTNCNDVSASYCGSTTGSPGSISATLIAENSEPDCCDANSQYFYTS
                     GAFDCPNGWPQIYWDDIPDGTYYYIVAMPDAVFYYDFVLEMNASECPPPQPGDNCNFP
                     LEISISGPGFAPYVDNNYTCGRGNFTSSTCLSLYDGGEDIFYEITVDAPVKCKFTMDP
                     QGTTYTGMALGASCPPPGTTYSNCLAEAHGSSSAPKSFVYEFTSPGTFYLMIDTWPTP
                     DCIPAFTLTIEELIPQPGD"
BASE COUNT          746 a          828 c          740 g          747 t
ORIGIN      
        1 atattatatt gacaaataat tagaaatctc tatattctta gtgtactggc cataaggtgc
       61 cagatgcact tctaatacat catcgattcg acaacgtggg agcaagtgca gggttaagct
      121 gttttgcgat ggcttgattt tcggcacggg attgcaatca atttggtact attatgccat
      181 tgagataaat gaccgaaaac gtggttaacc ttatcagtag cttagatgta attaaactaa
      241 ataagttcac aaactgcaca cacctgggat tttgcacctc gcgattgcaa taactggtac
      301 ttccctgatc tattgggagg agtagatcag gcaaaataca tatctgtgaa atattctacc
      361 ttttacccgc atggtgcctg gtagaagcag attaacaaaa tttagtttta acccgcctta
      421 agcgaaagga gagtcgatgc taagaggtaa caaattactg ctagcattac tgctgcctgt
      481 cttcgtactg gccttctgtt ttgctgcttt cgctcttgat gaagatgatt tcatcattat
      541 tgacggcgaa cattttacgc cggaacaatg ggccgcaaag caggaagcaa tggccgatat
      601 gaaggtcatg aaaactgatg tccagaagcc gattatctct caggctcctc aggatggcat
      661 tgatgtcaat gatgagaccc tgagatttaa tcccagacag catgagattg taaacaaggc
      721 cgctccggcc accaatctgc tgtttgagga cttcgagggt ggagttgtgc cgcccaccgg
      781 ctggactctg gatgcgcaga atactagcta cacctggaaa cttgcaactt atgatcccta
      841 caacggatca aattatacca actgcgagta tgatccggcg ctcacccctc aggatgagtg
      901 gctgatcagc ccggtcgtcg acctgaccac aagtggatcg acctggcagc tcgaattcca
      961 ttggtatatg agctattact ggggtgtgag tccctatgac aactatgatc tggaagtctg
     1021 gatctccacc gatggcggca cgaccttcag cacgatgctg tggaccgagc cgacaactac
     1081 attcaccaac tggacctggt atgatgtcat tattgacctg acaccatacc tgactgaaac
     1141 caacgttgca ttcggtttcc gttatgttgg ttcggatggt gccgagggtg cctttgacta
     1201 tatctcggtc aatgatactc ctccgcccac cggccgctgc tgctatggtg atcccaacac
     1261 tccatcctgt gcggatgaga gccaggctgc ctgtcaggca cgtccggatt ggctgtcatg
     1321 ggatgaaggc cttgattgta ccaacaatcc ctgcccgatc gtgggacagg gggatgactg
     1381 tacaaatcca atcacggcca cattcccggc cgatttcgca ggaactcaat atcttaataa
     1441 caattacacc tgcggcagaa tcgatgatta tgatgccacc gcctgtctcg gctactatga
     1501 cggcggcgaa gacataatct atgaattcac cgtgacccag gatatttata tcaacattac
     1561 cctggatccc aagggcacga cctggaacgg ctttgccctc ggaaccgact gcccgccgaa
     1621 cagctgcgta gctgtaagca ccagcagtgc aggttcacca cacggagtct cgaatgtaat
     1681 tttgactgcg ggaacgtact atgtgatggt cgacacatgg ccctcaccgg attgtatccc
     1741 caatttcgat ctgataatcg attatctggg tgatcctccg ccgacaccgg ataacgacga
     1801 gtgtgccaat gcagaactcg tagccggtcc tttcccgcag acggtgacag gctcgaccgt
     1861 gaattccact atcgactgcc ccggccttct ggactggtat gcagtatggt actacttcga
     1921 aaatccatat agctgcgcca acctggtgat cgactggtgc ggaaccacgc ctgacctcgc
     1981 agtggttggt gtcgttgtta tggatgactg tgcctgcgac gattacatcc tgcacaacaa
     2041 taccacctgg gatgagtgcg gcgatggcaa tccgaccaca tactggaacg gtctggcagc
     2101 gggcacatac tactatgccg ccttctccgg cccgaattca tccggtaccg gtcaaaccca
     2161 ggatcatact attacgtttg atctgcagga atgcccgccg gcaccgccta atgataattg
     2221 cgcaggcgcc gaggttatta cgcttggaac acccgtttca ggcactacag cgggcgcaac
     2281 accagactgc ccatcactct tcactgactg gtacggcgta tggtatcagt tcaccctcag
     2341 cagttccaca aactgcaatg acgtaagtgc aagctactgt ggttcaacta ccggcagccc
     2401 cggaagtatc agcgccaccc ttatcgctga gaactccgag ccggattgct gcgacgcaaa
     2461 ttcgcagtat ttctacacct cgggcgcttt tgactgcccg aacggctggc cgcagatcta
     2521 ttgggatgac attcccgatg gtacctatta ctatatcgtg gccatgcccg acgccgtttt
     2581 ctactatgac tttgtgctgg agatgaacgc ctctgaatgt cctccgcctc aacccggcga
     2641 caactgtaac ttcccgctgg agatttcgat cagtggcccc ggcttcgcac cgtatgttga
     2701 caacaactat acctgcggcc gaggcaactt cacaagctca acctgtttga gcctctatga
     2761 cggcggcgag gacatattct atgagatcac tgtcgatgct ccggtgaaat gtaaattcac
     2821 gatggatcct cagggaacca cctataccgg tatggctctg ggcgcttcct gcccgcctcc
     2881 gggaacaacc tacagcaact gcctggctga ggcgcatggt tcatccagcg ctcccaagag
     2941 cttcgtttat gaatttacaa gtcccggcac attctatctg atgatcgaca cctggccgac
     3001 accagactgt atcccggcct tcacactgac gatcgaggag ctgattcctc agcccggcga
     3061 t
//
LOCUS       DSEB01000291            2206 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1008153, whole genome shotgun sequence.
ACCESSION   DSEB01000291 DSEB01000000
VERSION     DSEB01000291.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1008153"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1167
                     /locus_tag="ENO07_05275"
     CDS             <1..1167
                     /locus_tag="ENO07_05275"
                     /inference="COORDINATES: protein motif:HMM:PF00266.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDS01415.1"
                     /translation="REIVAEFIDADLDKNVIIFTKNSTEALNKLARRYPFQRNDVVLT
                     SLMEHHSNELPWRNRAFFDHVGLKPDGTLSIEDLKEKLKFYNGRVKLVAITGASNVTG
                     YINPVYKIAKIAHDAGAEILVDAAQIAPHRPIHMNNNGMEDIDYLVLSAHKMYAPFGV
                     GVLVGSKSIFEIGDPSDVGGGVVDIVTLEEAYWTDLPEKEEAGTPDIIGVVALTESIR
                     MFQAIGWEDIIEHEAKLTAHLLRRLKEIDKIIIYGDADPANSHNRLGVVPFNMAGLSH
                     ALLAAILSYEGGIGVRNGCFCAHTYVKEILKVKAEEARKLEEEIKRRDRSHLPGTVRA
                     SFGIYNALEEVDRMCDLLEKISADGHKGKYILNPEKGEYMPEGFKIEVTDYLRL"
     gene            1295..1558
                     /locus_tag="ENO07_05280"
     CDS             1295..1558
                     /locus_tag="ENO07_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01416.1"
                     /translation="MESQKDQQADNYRSLKYLITGIDDMRDVISMIWGLINTKIPRDE
                     RVNMARMIIPGSVSLKQNSTSFILDLKVPPALMENKDAKRRKK"
     gene            1536..1853
                     /locus_tag="ENO07_05285"
     CDS             1536..1853
                     /locus_tag="ENO07_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01417.1"
                     /translation="MQKEEKNKAVRQVPRPEIKIAGKNTILTYHLVSFYELYLAISQI
                     NGMLSNISTRRDSFTIKRDIKIEASDEEAGPGITLRITIPKRINDMSLIGTAISSSQS
                     LSK"
BASE COUNT          636 a          498 c          561 g          511 t
ORIGIN      
        1 cgcgagatcg tggccgagtt tatcgacgcc gacctggaca aaaatgtcat aatatttacc
       61 aaaaattcaa ccgaggctct gaataagctg gccaggagat atccattcca gcgcaatgat
      121 gtggttttga cctcgctcat ggaacatcat tcaaatgagc tgccctggcg caatcgggcc
      181 ttctttgacc atgtgggcct gaaaccggat ggaaccttga gtattgagga cttgaaggaa
      241 aagctcaaat tttataacgg ccgggtgaag ctggtggcaa ttaccggggc ctccaatgta
      301 accggctata taaatccggt atacaaaata gctaaaatag cgcatgacgc cggcgcggaa
      361 atccttgtgg atgccgccca gatagcgccc catcggccga ttcatatgaa taacaatgga
      421 atggaagata tcgactacct ggtactttcg gcacataaga tgtacgctcc cttcggggtg
      481 ggggtgttgg tgggcagcaa gagtatattc gaaatcggtg acccctctga tgtaggcggg
      541 ggagtggttg atattgtgac gctggaagag gcttactgga ccgatctgcc ggagaaagag
      601 gaagccggca caccggatat catcggcgtg gtggccttaa ccgagagcat ccgcatgttc
      661 caggccatag gctgggaaga tattatcgag catgaggcaa agctgaccgc tcatttgctg
      721 agacggctga aggagatcga caagattata atctacggcg atgccgatcc ggctaacagc
      781 cataatcgtc tgggggtggt acccttcaac atggccgggc tttcccacgc gctgctggcg
      841 gccattttaa gctatgaggg cggcatcgga gtgagaaacg gctgtttctg cgcgcacacc
      901 tatgtaaaag agatcctgaa ggtcaaggcc gaggaagccc gcaagctgga ggaggaaatc
      961 aaacggcgcg accgcagcca tctccccggt acagtccggg cctcattcgg tatctataat
     1021 gcgctggagg aggtcgaccg catgtgcgat ctgctcgaaa agatcagcgc tgacggccat
     1081 aagggcaaat atattctcaa tcctgaaaag ggcgaatata tgcccgaggg attcaagatc
     1141 gaggttacag attatttacg gctgtgatga taatccactg aatttcaagc attagcctga
     1201 tcttttatca ctttttccgc tcaattgccg tttgatacct gccgatatcc taaagaaggg
     1261 gttaaagcgg gttattaaac gagaattttc ttatatggaa tcgcaaaaag accagcaggc
     1321 tgacaattac cgaagtctca aatatctgat aaccgggatt gatgatatga gggatgtaat
     1381 cagtatgatc tggggtctga taaataccaa aatccccagg gatgaaagag tgaatatggc
     1441 tcgaatgata atacccggtt cagtctctct caagcaaaac tcgacttcat tcatattgga
     1501 tttaaaagtt cctcccgccc ttatggaaaa caaggatgca aaaagaagaa aaaaataaag
     1561 ctgtcaggca ggtgccccgc ccggaaatca agatcgccgg caagaatacg attctgacct
     1621 atcatctggt ctctttctac gagctctatc tggcaatcag ccagatcaat ggaatgctca
     1681 gcaatatcag caccaggcgt gattcgttta cgatcaaaag agatataaaa atagaagcca
     1741 gcgatgagga ggccggacca ggcatcaccc tgcgcattac tattcccaaa cgcattaacg
     1801 atatgtcgct gatcggaaca gcaataagca gctcgcaatc attatcaaaa taaaatccgc
     1861 cgcttccata tactgctgac tttataatgc tgaattatcc tgaggtttct tttgcagaag
     1921 agtatcaaaa tcaggtcacg caagccgcgg caaacccggt ataatggcta agccatttat
     1981 tagcaacaga gtaacataag tgcgcgccgg gggtggagca aaaaagcaca gtatcgaggg
     2041 ctattttcct gttgccaaat atggtattaa tgatatatta aatgagaaga aatttacttg
     2101 cctgttgggc caatacacat atcctgctca tctattccgg ttccgggcaa acaatgttta
     2161 tatctgcagg agcgggggac aacgggcctt gatcgggaat atagcc
//
LOCUS       DSEB01000292            1973 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1010406, whole genome shotgun sequence.
ACCESSION   DSEB01000292 DSEB01000000
VERSION     DSEB01000292.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1010406"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            161..856
                     /locus_tag="ENO07_05290"
     CDS             161..856
                     /locus_tag="ENO07_05290"
                     /inference="COORDINATES: protein motif:HMM:PF13489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDS01418.1"
                     /translation="MEFTSSGRKQVPANDAEKVRDYFERASDLFNSIYTGEKSAFGRL
                     LDKLLRPDLKERFVLTLREIQHEKPQSLIDIGVGPGQYLKAYVELGVPRITALDFSKP
                     MLDLAENLVGEPPGGVQVSYVVADFMEYEPDQNYDISIAMGVFDYVADPETFLLKMKN
                     ISNRCVLASFPSISVWRTPIRKVRYAYKRCPVYFYNRARIETLARKIGFKDFEITKIR
                     GSGMDYFVRFDCD"
     gene            complement(853..1965)
                     /locus_tag="ENO07_05295"
     CDS             complement(853..1965)
                     /locus_tag="ENO07_05295"
                     /inference="COORDINATES: protein motif:HMM:PF00534.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase WbuB"
                     /protein_id="HDS01419.1"
                     /translation="MTALPNYPTGKIFDDYRNHYKYKENIDGVEVIRSWLYVHRSRNA
                     LSSALSYLSFALTAYDVGSREIASANLLLWEYPPPFLGHTAGKLAKRWNAGLVTNIAD
                     LWTHALEEQKVLKSRYILEKISAFEKSIMQKSMAVTGQTEGVLSDIKARLPESDPILW
                     PNGADPDLFRPMTPSSQMQTRYDVRGKFVVGYSGLHGRNHNLKLLLDAARILQPENDI
                     VFTLCGDGFEKQKLITYAMQNGLSNVHFHDPIPHSRLPELLSLFDVGVVIHRDLPGLK
                     VVRSAKLFELMSMQIPILHCPVSEGAEIVRKADAGLVVEEEDPRKIADTIISMRNSAK
                     LAEWGKNGRKHVIENFDRNEISLELVQKLEKLVGQA"
BASE COUNT          489 a          485 c          508 g          491 t
ORIGIN      
        1 cgcccggcgg ctgcatactg tcatgaacaa tcttctggag gaggagatgt tcagccttcc
       61 ggagggcggc gccaaaaaga tcaggatttc cagggagaag gtcaaaaatc gcctggctga
      121 aattgtcgag gacgaggatt tgagcaaata tattctctaa ctggaattca cctcttcggg
      181 gagaaagcaa gtgcctgcca acgatgccga aaaagttcgc gactactttg agcgagcctc
      241 agatcttttc aattcgatat atacggggga gaaatcggct ttcggaagac tgctggataa
      301 gcttctgcgt cccgacctta aagagagatt tgtcttgacc ctgcgcgaga tacagcacga
      361 aaagccgcag tccctgatcg atatcggcgt ggggccgggg cagtatctga aggcctatgt
      421 tgaactggga gtgcctcgga tcacagccct tgatttttca aaaccaatgc tcgacctggc
      481 cgaaaatctg gtgggtgagc cgcccggggg agtgcaagta tcctatgtag ttgctgattt
      541 tatggagtac gagccggatc agaattatga tatttcaatt gccatggggg tcttcgacta
      601 tgtcgccgat cccgagacgt tccttttaaa aatgaaaaat atcagcaacc gatgcgtgct
      661 ggcgagtttt ccctcgattt cggtctggcg cacccccatt agaaaagtgc gctatgccta
      721 caaacgctgc ccggtctatt tttataatcg cgcaagaatc gaaacgctcg ccaggaagat
      781 aggctttaaa gattttgaaa ttaccaagat caggggcagc gggatggatt actttgtaag
      841 gttcgactgc gattaagcct gcccgactaa cttttccagt ttttgcacca gctccagtga
      901 aatctcattg cggtcaaagt tctcgatcac atgcttgcgg ccgtttttcc cccattcggc
      961 gagctttgca gaattgcgca tggagataat agtatcggct atcttccgag gatcctcctc
     1021 ctccacgacc agaccggcat cagcctttcg cacaatctca gccccctcgc ttacagggca
     1081 atgcaggatt ggaatctgca ttgacatgag ctcaaacagt ttggcagacc tgaccacctt
     1141 cagtccgggc aggtcgcggt gaataaccac gccgacatca aacagagaga gaagttcggg
     1201 gagccgactg tgggggatcg gatcgtggaa atgaacattg ctcagcccgt tctgcatggc
     1261 ataggtaatc agcttctgtt tttcaaatcc gtcaccgcac aacgtgaaaa caatatcatt
     1321 ttctggttgc aatattctgg ccgcatccag gagcagcttc agattatgat tgcgtccatg
     1381 cagacccgaa tagccgacca caaatttccc tctgacatcg tagcgagtct gcatttgaga
     1441 ggagggagtc atgggcctga acaaatccgg gtcggcgcca ttgggccaca gaatcggatc
     1501 gctttccgga aggcgcgcct taatatcgga cagcaccccc tcggtctgac cggttacggc
     1561 catgcttttt tgcataatgc ttttctcgaa ggcgctgatc ttttccagaa tatatctgga
     1621 cttcaaaacc ttttgttcct ccagcgcatg agtccagaga tcggcaatat ttgtcaccag
     1681 tccggcgttc catctttttg caagtttccc ggcagtatgt cccaaaaagg gaggagggta
     1741 ttcccacagg agcaggttgg cggatgcaat ttctcgcgag ccgacatcat aagctgtcag
     1801 tgcgaagctg agatatgaca gcgctgaaga cagggcattg cgggatctgt ggacatagag
     1861 ccaggagcgg attacctcga ccccgtcaat gttttcctta tatttataat gatttctata
     1921 atcatcgaaa attttgccgg tgggatagtt tggtaacgcg gtcaataccg taa
//
LOCUS       DSEB01000293            2757 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004962, whole genome shotgun sequence.
ACCESSION   DSEB01000293 DSEB01000000
VERSION     DSEB01000293.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004962"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            1605..2327
                     /locus_tag="ENO07_05300"
     CDS             1605..2327
                     /locus_tag="ENO07_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HDS01420.1"
                     /translation="MSEKGCVIIPTYNERENVEEITKAVLGQDERLHVLIVDDSSPDG
                     TGEVADKMAQNNPRIDVLHRDKKEGLGQAYIAGFKHALQSDFDYIFEMDADFSHDPKY
                     LPYFLEAIKEADLVLGSRYISGVNVINWPMSRLLLSYYANVYTRIVTGLPLRDATGGF
                     KCFRRQVLEAINLDEVRSNGYSFQIEMSFRAHKKGFKIKEIPIVFTDRIQGTSKMSKK
                     IVREAIWMVWRLRFWSLFGKIK"
     gene            2324..>2757
                     /locus_tag="ENO07_05305"
     CDS             2324..>2757
                     /locus_tag="ENO07_05305"
                     /inference="COORDINATES: protein motif:HMM:PF00535.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDS01421.1"
                     /translation="MTPGLTSIIIINYNTADLTLRLLRSIYEYCRKDDFEIVLIDNAS
                     RDSSLEELQSSYPELVLIKNKTNSGFARAANQGGKASAGEYLWFINSDCELTMPVLDI
                     LKNALINTPDAAAVTPKTVDKSGNFHSVCRNFPSHKNILFSRG"
BASE COUNT          681 a          629 c          713 g          734 t
ORIGIN      
        1 atttgtgcgc aacaagagaa cctggttctt catgggactg gcgctgttcg ccctgatata
       61 tgcgatgggt ggatccacac cgctgtacag aatattttac catctgatac cgaacgtcaa
      121 gcacctgcgc gccgcttcga tgatcatgtt tattttctcg ttctccttct gcctcctggc
      181 cggatttgga atcgactatc ttcgcgaagc gctgccaaag ctcaaggatg ccgtaaaaag
      241 gaaggtgttc ctgttttctg gaattgccgc gggcttatat ggatttctgg ccgtcgcctt
      301 cagtctggcg gggaagagca taatgaaggc ctttgcatcg atttcgccgc cttatacgcc
      361 tcagggcgta tcgattgaca ctatacttga gtatcgcgcc tttcctaatc tcggcgacat
      421 cgtgataggg ctctggatcc tggctgcggt tttcgttctg acatatatca tcattaaagc
      481 atatggcaac cgcactatag gattgtgggc cctggggatc atagtatttt tcggcattgt
      541 cgatgcctgg cgtataaatt ttaaattcat taccacgacc agtcccgagc aatattttgc
      601 caccaataat gtagtttcta tgatgaggga taccgataaa ccctatgttg accggctgct
      661 tgatgccgac cggggagtgc tgcgcaactc caattacttt gcctaccatg atatacccat
      721 gatgtttgga taccacggca atcagatgaa gatttatgac cagtactggt tcaggcaggg
      781 caagtcgaac gatcactcat tcatctaccg aattgaaaac gacccgcaac agggcaatcg
      841 cggaaatatg gtctggctca atactccatt tataggaatg gctggtgtca agtatctggt
      901 gaccgattac atgaccgacc tgggcgttta ccgggacagc ctgactcaga tatcccagaa
      961 tagcgatctc caggctgatg gtctgatctt ataccagttt cccgacaact tcaagagggt
     1021 gcggctgtat catgattatg tgttgtccga ggattatggg catggtgtag attacctgag
     1081 gctagtctat tatgattatg tgctgcgcga ggctcggggc gaagttgtga attatctgcg
     1141 gatgctgtat gatgagtata tattgcatgg ggaacttgaa aatgagctga actatctgcg
     1201 aaccctccag catgattgga ccaggcaggt cgttctggag aaagatcctg gatttgcagc
     1261 ggattccgcg gccgttaccg agggcgagta tgccgagatc gcctcgtatg atactgatga
     1321 agtcgtaata aatgtaaaat ctaacactga cggaattgta tattttgcag gctgctatta
     1381 tcccgcctgg aaggcctatg tgaacgggga ggaggccgag gtattgcttg ccaatgccgc
     1441 cttcagggga gtcgtggttc cggaaggtga acacgaggtt gtgttcagat acgagtcgga
     1501 ggtttacaat aactcacgag ctgtgacttt ggcaagttca gctttcgtag ttctggtgct
     1561 ggcattcagt ctgttcaggt gccgcagagt ggaggagaaa gcagttgagt gagaagggct
     1621 gcgttataat tcccacttat aacgagcggg aaaatgtgga agagattaca aaggctgtgc
     1681 tgggacagga tgagcgcctg catgtcctga tcgtcgatga cagctcgccc gacggcacgg
     1741 gagaagttgc cgacaagatg gcccagaaca atccgcgcat tgatgttctt caccgcgaca
     1801 aaaaagaggg actgggccag gcctatattg ccggattcaa gcatgccctc cagtctgatt
     1861 tcgattatat ctttgagatg gatgcggatt tttcgcatga tcccaaatat ctgccttatt
     1921 ttctcgaggc gatcaaggaa gccgatctgg ttctgggttc gcgttatatt tccggagtga
     1981 atgttatcaa ctggcccatg agccgcctgc ttctgtctta ttacgccaat gtctatactc
     2041 gaatcgtgac cggtttgcct ttgcgggatg ccaccggcgg cttcaaatgt tttcggcgtc
     2101 aggtgcttga ggctatcaat ctggatgagg ttcgttccaa cggttattct ttccagattg
     2161 aaatgagctt ccgggcccat aagaagggct tcaaaatcaa agaaattccc atagttttca
     2221 ccgatagaat acagggcaca tccaaaatgt ccaaaaagat tgtgcgcgag gcgatctgga
     2281 tggtttggcg gctgaggttc tggtcgctgt ttggtaaaat aaaatgactc ccggcctgac
     2341 cagcataatc atcattaact acaacaccgc cgatctgaca ttaagacttc tccgatcgat
     2401 atatgaatac tgccggaaag acgatttcga aattgtgctg atcgataatg cttcaaggga
     2461 ttcatcgctc gaggaacttc aaagcagcta tcctgaactc gtattgataa agaacaaaac
     2521 taacagcggc ttcgcccgag ccgccaatca gggaggcaaa gcgtccgcag gcgaatacct
     2581 gtggtttatc aattccgatt gcgagttgac catgcccgtt ctggatatcc tgaaaaatgc
     2641 attgattaat actccggatg ccgctgccgt gacaccgaag acagttgata aaagcggtaa
     2701 tttccattca gtctgccgaa attttccgag tcataaaaac atcctgttct cacgcgg
//
LOCUS       DSEB01000294            2758 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004961, whole genome shotgun sequence.
ACCESSION   DSEB01000294 DSEB01000000
VERSION     DSEB01000294.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004961"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1062
                     /locus_tag="ENO07_05310"
     CDS             <1..1062
                     /locus_tag="ENO07_05310"
                     /inference="COORDINATES: protein motif:HMM:PF13205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01422.1"
                     /translation="TITNSGSGVLNWNATKTQTWLNLSPASGVAPSNMQIAVNTSGLS
                     VGTYYDTITVTGAGATNSPQVAEVILTLNPATPLVLTTDPADGATEVPIDALISASFS
                     IDMDGSTINESSMLVRTGGGEYYGGTVNYIPLSRTAAFVCDSNFDKDQRIYVDLTTDI
                     QSSQGMSLGGTYTFDFFTVTNNPPNTPSDPFPADDAVDVPITADLSWSGGDPDGDDVE
                     YTLYFGQQNPPQSYATSLTEPTFDLPELENETHYYWYVKAFDGYGGIASMPICDFTTE
                     IGYICGDANGDLTVNVSDAVSIINFVFVGGQEPDPYQSGDTNCDGSINVSDAVWIINF
                     VFVGGNDPCDTNGDGEPDC"
     gene            1260..2009
                     /locus_tag="ENO07_05315"
     CDS             1260..2009
                     /locus_tag="ENO07_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDS01423.1"
                     /translation="MLEFKGKKALVTGSTRGIGLETARILAKGGADVCVNYFRSRKNA
                     DGAVEELKSYGSDAFAVRANVGNHEHIPPIFEQIKERWGKLDILVSNAALGLPTKAIE
                     MTDKAWHLSMHTNAQALLVLVQKGEFLMPDGSKIVALSSLGSNRYIPGYAAIGVSKAA
                     IETLTKYLAYELAERRINVNTVSGGFIDTDALKSFESYEHMLNEAIRRTPYGRAGKPE
                     EVAEVVAFLCSDRASWVTGQVLVVDGGYSLA"
     gene            2063..>2758
                     /gene="alr"
                     /locus_tag="ENO07_05320"
     CDS             2063..>2758
                     /gene="alr"
                     /locus_tag="ENO07_05320"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDS01424.1"
                     /translation="MNNTKISTSLSWIEVSRENILNNIRVIRKKIGPDIKIAAVVKAN
                     AYGHGYHEIAQILEDEQIEFLAVHSLEEAFILNEGGYSPELLIVGYVPYDQLNEAVES
                     GFHLTVYNTDTIRQLEKLKTSKKAKIHLKLETGTNRQGIDEKELPSFIGLLKNTKKID
                     LIGASTHFANIEDTTDHSYAQSQLDRYKEILAEIENAGLEIPIKHTASSAASLLFSKT
                     HYNMIRFGISLYGL"
BASE COUNT          746 a          672 c          670 g          670 t
ORIGIN      
        1 acaataacaa actccggcag tggagtactc aactggaatg ctaccaaaac tcagacctgg
       61 ctgaacctct cgcccgcgag cggtgtggcg cccagcaata tgcaaatcgc ggtaaacacc
      121 tccgggcttt cggtgggaac ttattacgac actatcacag taacaggcgc tggagcgaca
      181 aattcccctc aggtcgcgga agttatactt acgttaaatc cggcaacacc tcttgtgtta
      241 acaaccgatc ctgccgacgg cgctaccgaa gttccgatag atgccttaat ctcggcctcc
      301 ttctcaattg atatggacgg ctccacaatt aacgaaagca gtatgctggt gagaactggc
      361 ggaggtgaat attacggcgg cactgtcaac tacattccct tatcgagaac tgcggctttt
      421 gtatgtgaca gcaatttcga taaggatcag aggatatacg tggatctgac aacagatatt
      481 caatcctcgc aggggatgtc gctcggcggc acttatacat tcgacttctt caccgtgacc
      541 aataatccac ccaatacgcc gtccgatccg ttcccagccg atgacgcagt cgatgtaccg
      601 atcacagcag acttgagctg gagcggcggt gatcctgacg gagatgatgt cgaatatacg
      661 ttatatttcg gccagcagaa tccgccccag tcttacgcta ccagcctgac cgaaccgact
      721 ttcgacctgc ctgaactcga gaacgaaaca cattactact ggtatgttaa ggcatttgac
      781 ggatacggcg gaattgcctc catgccgatc tgcgatttca ctactgaaat cggctatatc
      841 tgcggtgatg ccaatggtga tttgactgtg aatgtcagcg atgcggtttc tatcatcaat
      901 tttgtctttg tgggcggtca ggagccagat ccctaccaat cgggtgacac caattgcgac
      961 ggatcaatta atgtgagtga tgcggtctgg atcatcaact tcgttttcgt gggtggaaat
     1021 gatccctgcg ataccaatgg cgacggggag cccgactgct aaaacgtgag ttctatatat
     1081 agcctgctcc gaaacccgcc cagacatggg cgggtttttt aatgctgtaa gacatccagg
     1141 cctgatattt ccaatgtttt gcggaacaag atgtctatta tcctgttttt ttgcaaacaa
     1201 tgattgacat attaaactat taagagtata attgtccaca ttacatcatc aaggaggaga
     1261 ttttggaatt caaaggtaag aaggcgcttg ttaccggatc gacccgaggt atcggtcttg
     1321 agactgcacg tatactggca aagggcggag ctgatgtctg tgtaaattat ttccgttctc
     1381 gcaagaatgc cgatggtgca gtcgaggaat tgaaatcata cggttcggat gcattcgctg
     1441 tcagggccaa tgtgggcaat catgaacaca tcccccccat cttcgaacag atcaaggaac
     1501 gatggggcaa actggatatt ctggtttcca atgcggcact gggcctgccc actaaggcga
     1561 tcgagatgac cgacaaggcc tggcatcttt ccatgcatac caacgcccag gcgcttctgg
     1621 ttctggtcca gaagggcgag tttttgatgc ctgacggttc caagatcgta gccttatctt
     1681 cgctcggatc caacaggtat atccccggtt atgcggccat aggtgtctcc aaggccgcaa
     1741 tcgagactct cacaaagtat ctggcatacg aacttgcgga acgcaggatc aacgtgaata
     1801 ccgtttcagg cggttttatc gacaccgacg ccctgaagag cttcgaaagc tatgaacaca
     1861 tgcttaacga agcgatccgc cgcaccccct atggccgggc aggaaaacct gaagaggtgg
     1921 ccgaggtggt tgccttttta tgcagcgaca gggccagctg ggtaaccggc caggtgctgg
     1981 tcgtagacgg aggatattca ctggcataat cggttttgtg ttaaaatttg acttatacct
     2041 ttaacagcat atattcagcc acatgaataa tacaaaaatc tcgacatcat tgagctggat
     2101 cgaagtcagc cgggagaata ttctcaataa tatcagggtc atccggaaaa aaatcggtcc
     2161 tgatatcaag atagcggcgg tggtcaaggc caacgcttac gggcacggct atcatgaaat
     2221 tgcacagatt cttgaggacg agcagatcga atttctggcg gtccatagcc tggaagaggc
     2281 attcattctg aatgagggcg gatactcgcc cgaacttctg atagtcggct atgtaccgta
     2341 tgaccagctc aacgaggcag ttgagtcagg atttcatcta accgtctata acactgacac
     2401 tatcagacaa ctcgagaaac tgaagacatc aaaaaaagcg aaaatccacc ttaagctgga
     2461 aacgggaaca aatcgtcagg gaattgatga aaaggaactg ccttccttta tcggactttt
     2521 aaaaaataca aaaaagatcg atctgatcgg agcatccact cattttgcga atatcgagga
     2581 cacaacagat cattcctatg cacagtctca gctcgaccgc tataaggaga ttttagctga
     2641 aatagagaac gccggtctcg agatcccgat aaaacatacg gcctcttcag cggcctccct
     2701 tctgttttca aagacccact ataatatgat caggtttggc atttctctgt atgggctc
//
LOCUS       DSEB01000295            6151 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000839, whole genome shotgun sequence.
ACCESSION   DSEB01000295 DSEB01000000
VERSION     DSEB01000295.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6151)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000839"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            405..2771
                     /locus_tag="ENO07_05325"
     CDS             405..2771
                     /locus_tag="ENO07_05325"
                     /inference="COORDINATES: protein motif:HMM:PF04371.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01425.1"
                     /translation="MPVMSLLLQKSKLIVGSMIFLSFLLTLSTAAQSGEGFNRFEEEK
                     QAELGLLPRWRDGGTPYDRSQSLEPFKIVLPDKDMPTSGYISSPPEYSPSRGVLFSYF
                     SSGWPGVVRDMVVALTSDPDYDEIAYVCVANSSQQSSAYSTFSSAGADMSKVEFIIEP
                     MNALWIRDYGPHLIWQENTLGIVNSQYYPTRPLDNFIPELVGTDHFQIFTYDMGLYYS
                     GGNFQPTQNRNGFVTALIQLDNTASGGFDANFIAELYHTYQGIDTLHIMPQLPFSVDG
                     TGHIDMWMYIIDEDDVIISEFLPGSDPTAIAVTNNAVPYMESLGYTVHRTPAWNSSHP
                     STGYWTHWTYTNALRVNNRILIPSYGDTYLDYADEDAQALAVWEAAAGPDVEIIPINS
                     YDIIWAAGALHCIAMQVPRYEEPAPSANIIWPDGGELFAGGTVETIQWEATDSNNAEL
                     SEAVLYYSVDDGAGYILIDTVPDTGNYDWTVPDIYAEQCRIKVVAVSQDSDEYEAVSA
                     GPFEIASCQQSVYDFSTGAGVDKFAFGHQTSNWAAVSGIRTPVNTEIENLISGAYDKI
                     AHSDATGNDYDPNRYLSLDPNNGWESTHIFEFTLTEDVSEVDDIEVFWEGYSDQCSMM
                     EMYIWDYTSGEWCDGEGLFDQNRFMDNWAGNRDGYLEKHIRSDFGRFVDGSGQLTVLL
                     YSERGPDGSYVNYNPTLHDYISVTVSTKLPQYMCGDADASEAVNVSDAVMITNYIFVG
                     GAAPEPFESADTNCDGTVNISDVVVIINFVFLDGNTPCDTDGDGEQDC"
     gene            complement(3272..3730)
                     /locus_tag="ENO07_05330"
     CDS             complement(3272..3730)
                     /locus_tag="ENO07_05330"
                     /inference="COORDINATES: protein motif:HMM:PF07238.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HDS01426.1"
                     /translation="MKVLDQDMQPKVRRKAAETLTAFDLNTRQPMGIVVDMSARGMKL
                     KSEIPATVAEVYYCRIPLKNKVDGRDEVFFDAECRWCKKYPETGRFYSGYILRFPSKK
                     DAAVVRKLIHDWMVRYNEELNSRYMAPEEDAEEAEDRRGLLTRLFKLGEK"
     gene            4076..5581
                     /locus_tag="ENO07_05335"
     CDS             4076..5581
                     /locus_tag="ENO07_05335"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HDS01427.1"
                     /translation="MLMPKLTNLIAVLALFLFLLAGCSQQDNPAGAVDSETKEISDDA
                     WVKIPAAAGLDSAFLFLTVSMNNGETVYVHRITSAWNEANVTWDNFNSSYDELPEAAF
                     VPDSYAEIRIDVTSLAAKWFNGVYPNHGMLLRQGASVSQYRSSEFFEVSSRPRLELFV
                     STPSGPQSFGIQETGLGEAEDAFIGGNFPTGNNGSADMLMVGNTFGFEQNALIKFNFV
                     VEEPEEYCSIGDLVWEDLNFNGLQDPGEPGLADVRVDLYSCDGQMVEGALTTPEGYFL
                     FDSLELGEYYLIFHIPPDYVFSIYDQGGNDNIDSDADPAMGATVCFAITAPGKYTSWD
                     AGFYLPTIDDDDDDGCTRGMGYWKNLCRNPSDKLITDDAGVFLPLWLGTEGGQNSIRI
                     GTIDEAEQIFTFEDDKTSNGIVKLCAHLLAAKFNLANGAGGDDISQELADADQFLAQY
                     GFEHWQEMSDEDRRLILIWKDTFEAFNEGEIGPGACPEDQDGDDIESSSAH"
     gene            complement(5674..>6151)
                     /locus_tag="ENO07_05340"
     CDS             complement(5674..>6151)
                     /locus_tag="ENO07_05340"
                     /inference="COORDINATES: protein motif:HMM:PF12844.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDS01428.1"
                     /translation="SRIKNNNYQIIILDIIMPGVDGVKLLQSIKGLDSDLCVIMLSGF
                     PTFERARETFKNGCFDFITKPFENEALLETINGEIKEYGFITNLNQKATSIIAQRVHD
                     LRAERKLSLRQLANRTGLSPSLIYQVEHGQTTPSIATISRIASALNSEMEYFFKGI"
BASE COUNT         1502 a         1501 c         1538 g         1610 t
ORIGIN      
        1 ctcaacaact gaatgataac tgctgacaaa actaagcgca tctcaaaccc tcctggttat
       61 ttttgttcag attaatcctg attatgtaag cacttacgta atataaaacg aaaagttaca
      121 tattgccggc ctcccgctgc ctatatggtt tttcgaatac agctcaaaat atagggcttg
      181 ccgggcttgt tctccaaata attaatcttg actggaaaag aaaattcagt ataataatca
      241 ggtacgcacc tttcgcccga atttatcggg gatcactgcc atttgtgact ttgcgaagcg
      301 gattttaata gtccgggttg tttcatgaac cccaggcccg tgatgaggta ctctatagcc
      361 tgtcaacaat ttcaatatga aaactctcaa tccaagcagg aggtatgccg gtgatgtcgc
      421 tgttattgca aaaatcaaaa ttaatcgtcg gatcgatgat atttctttct ttccttctaa
      481 ccctttcaac agccgctcaa agcggggagg ggttcaacag gttcgaagag gagaaacagg
      541 ctgaattggg cctgctcccg cgctggcgag atgggggcac gccctatgat cgcagccagt
      601 cgctcgagcc attcaagatc gtcctgccgg acaaggatat gcctacttca ggatatatat
      661 cctccccgcc ggaatattct ccctcacgcg gcgtcctctt cagctatttt tcaagcggtt
      721 ggccgggagt tgtccgggat atggttgtcg cattgacttc ggatccggat tatgatgaga
      781 tcgcctatgt ttgtgtcgcc aactcttctc agcagagcag cgcctattcc acattctctt
      841 cggcgggggc ggatatgagc aaggtggagt ttataatcga accgatgaac gcactctgga
      901 tcagagatta cgggccgcat ttgatctggc aggagaatac cctgggaata gttaacagcc
      961 agtactatcc cacccgcccc ctggataatt tcatcccgga gctcgtgggc accgatcatt
     1021 ttcagatctt cacctatgat atgggacttt actactccgg gggaaatttt cagccgaccc
     1081 agaaccgaaa cggttttgta actgctctaa tccaacttga taataccgcc agcggagggt
     1141 tcgatgccaa ctttattgcc gaactctatc atacctacca gggcattgac acactgcaca
     1201 taatgcccca gcttcccttc agcgtggacg gcaccgggca tatcgatatg tggatgtaca
     1261 ttattgatga agatgatgtc atcatatccg agtttcttcc cggctccgat ccgactgcaa
     1321 tcgccgtaac aaacaacgcc gtgccatata tggaatccct tggatatacg gtgcatcgca
     1381 ctccagcctg gaattcctct catccatcca ctggatactg gacacactgg acctatacca
     1441 atgccttgcg agtcaataac cgtatactca taccgtcata tggcgacacc tatctggact
     1501 atgccgatga ggacgctcag gcgctggcag tctgggaagc tgcggccggc ccggatgttg
     1561 aaatcattcc cataaattca tacgatatta tctgggcggc gggcgcgttg cattgcattg
     1621 ccatgcaggt gccgcgctat gaggaacctg cgccctcggc gaatataatc tggcctgacg
     1681 ggggagagct gtttgcgggc ggtactgtag agacaatcca gtgggaggcc acagattcga
     1741 ataatgctga gctctcggaa gcagtgctgt attattctgt ggatgatggt gcaggttata
     1801 tcttgataga tactgtgccc gacaccggca attatgactg gactgtacca gatatatacg
     1861 ctgaacaatg caggataaaa gtggtcgctg tctcgcagga cagcgacgaa tacgaagctg
     1921 tctccgcggg ccccttcgaa attgcctcct gccagcagtc ggtctatgat ttttcgaccg
     1981 gggccggcgt ggataagttc gcctttggac atcagacctc aaactgggct gccgtcagcg
     2041 gaatccgtac tccggtcaac acggaaattg agaatctgat cagcggcgcc tatgataaaa
     2101 tcgctcattc tgacgccacc ggcaatgatt atgaccccaa ccgctattta tccctggacc
     2161 cgaataatgg ctgggaatca acccatatct tcgaattcac tctgacagag gatgtctccg
     2221 aagtcgatga tattgaggtc ttctgggagg gatattccga ccagtgctca atgatggaaa
     2281 tgtatatctg ggactacaca tccggggaat ggtgtgatgg tgaaggatta ttcgaccaga
     2341 accgttttat ggacaactgg gcaggaaatc gcgacggtta tctggaaaag cacatccgtt
     2401 cagatttcgg ccgttttgtg gacggcagcg gacagctcac cgtgctcctg tattcggaac
     2461 gcggccctga cggtagctat gtcaattaca atcctaccct gcatgactat atatcggtaa
     2521 ccgtttcaac caagctgccc cagtatatgt gcggcgatgc tgatgccagc gaggctgtaa
     2581 acgtatcgga tgctgtcatg ataacgaatt atatatttgt cggcggggcc gctccggagc
     2641 cgtttgaatc cgccgacacc aattgtgacg gaacggtaaa tatttcagat gtggttgtta
     2701 ttatcaattt cgtttttctc gacggcaata cgccatgtga taccgatggc gatggagagc
     2761 aggattgctg agccaattaa atatatagca taaacataag ccgatcccgg ttcccgggat
     2821 cggcttttgc gtggctgaaa ttttttcaat taaaccttgc tcaacgggtt tgatttccgc
     2881 atcttaacag ggcataggtg tccacattgc agcaaagatc agcaccatgg acggtcacta
     2941 tattctttca gggaaggagg tgaaacccga tgaaagctgc gcgaaaagtc aaggcactgg
     3001 ctttattcct cgcgctaatc gcgctcggcg ggctggtgtt cagcgccgtg accgccgaaa
     3061 cggttacggc caaaccgtgc tgctgggtaa tggtttgcac ggtaacgccc ccgattgtct
     3121 gctgggaggt gtgtgtgccg tgcccgacgc tgccgccgcc gcccggctca tagcgccttt
     3181 tttgggtcta ttcgggaagt attgaggaag tcttttcaat tgggactttt tcagcaagca
     3241 cgccgatccg ggatcggcgt gcccttattt tttatttttc gccaagttta aatagacgtg
     3301 tcagcaatcc ccgtctatct tctgcctcct ccgcgtcctc ttccggagcc atgtaacggg
     3361 agttcaactc ctcgttatat ctcaccatcc agtcatgaat caatttcctg actacggcgg
     3421 catctttttt tgatggaaat ctgaggatgt agccggaata gaacctgcct gtctcagggt
     3481 atttcttgca ccagcggcat tcggcatcaa aaaagacctc atcacgccca tcaactttat
     3541 ttttcaatgg gatgcggcaa taatatactt cggccacggt cgccggaatt tcgcttttga
     3601 gcttcatgcc gcgggcagac atgtcgacca ctatgcccat tggctgccgc gtattcaaat
     3661 caaatgctgt cagcgtttca gccgctttac gcctgacttt tggctgcatg tcctgatcta
     3721 atactttcat aatactacat agacctcctc atcatttcta tcggaaaaaa acggctcaat
     3781 ttgaagaatt atgactgatt gtcaaattag atacagacct gtttaagatc tgccgggact
     3841 gtgctgaaaa taagcaccag gggcatgttt gagttttgga catctttacc ccgcgctgtt
     3901 caagccgcgt ggtcattaaa agtgcataga gtataatgat ttaactatct gcatccggcc
     3961 ccaaagatgt cagttattgc ttccgatatt tttaatggat agacacaaca tagcttttga
     4021 ttgacaattg tagacccagg ccctatggcc atggatttta atcagaaaag aggtgatgct
     4081 tatgcctaag cttaccaatt taatcgcggt tttagcgtta ttcctgtttt tactggcggg
     4141 atgttcgcag caggataatc ctgcaggggc tgtcgattcg gagacgaagg agatctccga
     4201 tgatgcctgg gtgaaaatac cggccgcggc tggactcgac tcagcatttc tatttctgac
     4261 ggtgtctatg aacaacggag agacggttta tgtccaccgc atcacatctg cgtggaatga
     4321 agcgaatgtg acctgggaca atttcaacag cagttacgat gaacttcctg aggccgcttt
     4381 cgtccctgat tcgtatgctg aaatccgcat tgacgtgact tctctggcag ctaaatggtt
     4441 caatggtgta tacccgaacc acgggatgct gctgcggcag ggagcatcgg tatctcaata
     4501 ccgcagcagt gaattctttg aggtttcctc acggccccgg ctggaattat tcgtctccac
     4561 accatccggc ccgcaatcat ttggtatcca ggaaactggt ttgggagagg cggaggatgc
     4621 tttcataggc ggtaatttcc ccacgggaaa taatggcagc gccgatatgc taatggttgg
     4681 aaatacattc ggtttcgagc agaatgccct gattaaattt aacttcgtcg ttgaagagcc
     4741 cgaggagtat tgttccatcg gggacctggt ttgggaggac ctgaatttca atggccttca
     4801 ggatcccggc gaacccggac tggcagacgt cagggtcgat ctgtacagct gtgatggcca
     4861 gatggtcgaa ggcgccctga caacgccaga ggggtatttc ctgttcgata gtctcgagct
     4921 gggcgaatac tacctgatct tccatattcc gcccgattac gttttcagta tttatgacca
     4981 gggaggcaat gacaatatcg atagcgatgc cgacccggcc atgggggcaa ctgtgtgctt
     5041 cgctataact gctcccggta aatacaccag ttgggatgcc ggattttatc tgcccactat
     5101 tgatgatgac gatgacgacg gttgcacccg tggtatgggc tactggaaga atctgtgccg
     5161 caatcccagc gataagctga ttactgatga tgctggtgtc tttcttccgc tctggctcgg
     5221 taccgaaggc ggccagaaca gcatcagaat cggcaccatt gatgaagccg agcagatctt
     5281 cacctttgaa gatgataaaa cctcaaatgg catagtaaaa ctctgtgccc atttgctggc
     5341 tgccaaattc aacctggcca atggagccgg cggggatgat atctcgcagg agctggcaga
     5401 tgccgaccaa ttcctggcgc agtatggttt cgaacattgg caggagatga gcgacgaaga
     5461 ccgaagactt attttaatct ggaaggatac atttgaggcc ttcaacgagg gtgaaattgg
     5521 accgggagct tgccccgaag atcaagatgg agatgatata gaatcaagca gcgcccactg
     5581 acccggtaag ggaagacagg accccataga aaaggcggag acaatccttg tctccgcctt
     5641 taatattatt tattggcagt ggcggggatc atttcatatg cccttaaaga aatactccat
     5701 ctccgaattc agggcagagg cgattcttga aatggttgcg attgaaggag ttgtctggcc
     5761 gtgttcgacc tggtagatga ggctgggcga caatcctgtt cgattggcaa gctgccgtaa
     5821 gctcaacttc ctctcggcgc gcagatcgtg cacccgttgt gcgataatgc tggtcgcctt
     5881 ctggttcaaa ttggttataa acccatattc ttttatttcg ccgttgatag tttccagcaa
     5941 tgcttcattt tcaaaaggct tggtgataaa gtcgaagcag ccgtttttga aggtctctcg
     6001 cgcccgttcg aacgttggaa atccagagag catgatcacg cataaatcac tatccagccc
     6061 cttgatactc tgcaatagct ttacaccatc cacaccgggc atgatgatat caaggataat
     6121 aatctggtaa ttattattct ttatgcggga g
//
LOCUS       DSEB01000296            1771 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1013259, whole genome shotgun sequence.
ACCESSION   DSEB01000296 DSEB01000000
VERSION     DSEB01000296.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1013259"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..237)
                     /locus_tag="ENO07_05345"
     CDS             complement(<1..237)
                     /locus_tag="ENO07_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01429.1"
                     /translation="MILILSGALLAEDRLFRFPDVSEDMIAFSYAGDIWTVDIEGGLA
                     RKITTDRGLEIFPKFSPDGRSIAFTGQYDGNTHVY"
     gene            407..814
                     /gene="acpS"
                     /locus_tag="ENO07_05350"
     CDS             407..814
                     /gene="acpS"
                     /locus_tag="ENO07_05350"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-[acyl-carrier-protein] synthase"
                     /protein_id="HDS01430.1"
                     /translation="MNEENLQSSFFEKNVSLGVDIESIERFKEMISRFKRSTLKRIYT
                     ETELKYCFSKINPAPSLAARFAFKEAAFKALSPLGERIYHRQVEINNSSSGAPQARFV
                     SEELNSKYLLKVTLSHSRHDAIAFVAAIKRDDS"
     gene            complement(811..>1771)
                     /gene="kbl"
                     /locus_tag="ENO07_05355"
     CDS             complement(811..>1771)
                     /gene="kbl"
                     /locus_tag="ENO07_05355"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015132662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDS01431.1"
                     /translation="KKLEAKIAEFLGMEDTILYSSCFDANGGLFETLLDENDAVISDA
                     LNHASIIDGIRLCKAERRRFAHNDMNDLEKHLKDTQNKKVRMVATDGVFSMDGDIARL
                     KDICDLAEKYDAMVMVDDSHATGFVGDTGRGSYEFRGVEGRIDIITSTLGKALGGASG
                     GFTSGKKKLIELLRQRSRPYLFSNTVAPVVVGVSMEVLEMLTTTSDLRQKLNENTKYF
                     REKMTDAGFDIREGIHPIVPIMLGEARLAKNIASDMFKEGIYVIGFSFPVVPRGQARI
                     RVQLSAAHEKEHLDKAINAFVKIGKKYDIIGKKMDEIVEMFKS"
BASE COUNT          456 a          466 c          399 g          450 t
ORIGIN      
        1 atatacatga gtgtttccgt catattgacc ggtaaaggca atactccggc catcaggaga
       61 gaattttggg aatatttcga ggcccctgtc ggtggtaatc ttgcgcgcca agcccccttc
      121 aatatccaca gtccagatat ccccggcata ggaaaaggcg atcatatctt cggaaacatc
      181 cggaaatcta aacagcctgt cctcggccaa aagcgcgccg gacagaatta gaatcatgaa
      241 gatagtgatg caaatcttgt aaacgtatga cattttacct ccaatatatt tttcacagta
      301 ttcatgactc acgaaagcag ggtcgtaaat catattatat tttctcccgt atattgactt
      361 ccgctgatga tctaactatc attccggcaa agacagtaat tataatatga atgaggaaaa
      421 tttgcaaagc agcttttttg aaaagaacgt tagcctcgga gtggatatcg aatcaattga
      481 gcgcttcaag gaaatgatta gtagattcaa gcgctccaca ttaaagagga tttataccga
      541 gactgaattg aagtactgtt tttccaagat aaatcccgct cccagcctgg ccgcccgctt
      601 tgcattcaag gaagctgcct tcaaggccct gtcaccattg ggagaaagaa tctatcaccg
      661 ccaggtcgag ataaacaaca gctcctccgg cgcgccgcag gcccgcttcg tgtcggaaga
      721 gctgaattcc aaatatctct tgaaagtaac tctctcgcac agccgccatg acgccatcgc
      781 atttgtggcg gcaattaaaa gagacgattc ttaggacttg aacatctcca caatctcgtc
      841 catcttcttg ccgattatat cgtatttttt cccgattttc acgaaggcgt tgatggcttt
      901 atccagatgt tccttctcgt gggcggcgga gagctggaca cggatcctgg cctggccgcg
      961 gggcacgacc gggaatgaga acccgataac atagattccc tccttgaaca tatccgaggc
     1021 aatattcttg gccaggcgag cctctcccag catgatcggc acgatggggt gaatgccctc
     1081 cctgatatcg aaaccggcgt cagtcatttt ctcgcggaag tatttcgtat tttcattaag
     1141 cttctggcgc agatcggagg tcgtggtaag catctccagc acctccatgc tgactcccac
     1201 caccaccggg gcaactgtat tggagaaaag ataggggcgc gatctctgtc gcaggagttc
     1261 gatcagcttc ttcttaccgc tggtgaatcc tcccgaggct ccccccagag ctttgcccag
     1321 ggtactggta ataatatcga tcctgccttc gacgccgcgg aattcatagc tgcccctgcc
     1381 ggtatccccc acaaatcctg tggcatgaga atcgtcaacc ataaccatgg catcgtattt
     1441 ttcggccagg tcgcagatat ccttcagcct ggcgatatcg ccgtccatgg agaagacccc
     1501 atccgtggcc accatgcgca ctttcttatt ttgagtatcc ttaaggtgtt tttccagatc
     1561 gttcatatcg ttatgggcaa aacggcgccg ttcagccttg cacaggcgga ttccgtcgat
     1621 aatgctggca tgatttagag catcggaaat aacggcatca ttctcatcca gaagcgtctc
     1681 gaacaacccg ccattcgcat caaagcagga ggaataaaga attgtatctt ccatacccag
     1741 aaactcggca atctttgcct cgagcttctt a
//
LOCUS       DSEB01000297            2736 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1005042, whole genome shotgun sequence.
ACCESSION   DSEB01000297 DSEB01000000
VERSION     DSEB01000297.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1005042"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..595
                     /locus_tag="ENO07_05360"
     CDS             <1..595
                     /locus_tag="ENO07_05360"
                     /inference="COORDINATES: protein motif:HMM:PF13489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDS01432.1"
                     /translation="ESITDIAEDLFIAGIFLEEYARDEKIFCDVCNGLGVNFEKASVL
                     DIGCGTGFYTDILERQGVKDYTGVDITNHHFEKLRERFPDYSFIKKDITSEKHEGDYD
                     LIIMIEVLEHIVNDDKLDFAMENVKRCLKENGVFLISSIWEKGRKHMFYVRKWTLDEI
                     RKRFRDYHFSEPIPFRGSYMVAIRKSQQSDKSFINNK"
     gene            731..1156
                     /locus_tag="ENO07_05365"
     CDS             731..1156
                     /locus_tag="ENO07_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01433.1"
                     /translation="MKKMIVLLLLAGLAASLVITCNETRIINTNEDDLILKKAAAFSI
                     ILPSQMVAGEDMQFRIILDAGFPCYTFARYDIEEVGGEIFITPMVYQRGTSCTNYTSR
                     IDRSMFYRIDEPGKYLFHFYESELSYKHYEIEVEPASGE"
     gene            1273..1650
                     /locus_tag="ENO07_05370"
     CDS             1273..1650
                     /locus_tag="ENO07_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01434.1"
                     /translation="MWNSGEKYEGAYLGTEMDELWWKRYKKDKIFARGNGKFWFDEMG
                     IYFHRYLTRRPLYIPFNQMRSFKIGQWHAGRWAMHIPILKIIWYKDNQKLSSGFALTK
                     KRRELAKLLEIFQQKTSAVFENV"
     gene            complement(2047..2481)
                     /gene="rpiB"
                     /locus_tag="ENO07_05375"
     CDS             complement(2047..2481)
                     /gene="rpiB"
                     /locus_tag="ENO07_05375"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_602674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HDS01435.1"
                     /translation="MKIALGADHKGYPLKEKIKRFLTERHDEVLDFGTDAEESCDFPD
                     FGLKVAHAVADGIVDYGINICWTGNGMIMAANKVKGIRAGLCLNAEMAKLTRLHNDAN
                     VLSLASKYVPEEDALEIVETFLTTDFEGGRHIQRLEKIAAEE"
BASE COUNT          758 a          650 c          592 g          736 t
ORIGIN      
        1 cgagtccatt acagacatcg cagaagatct tttcatcgcg ggcatattcc tcgaggaata
       61 tgcccgcgat gaaaagatct tctgcgatgt ctgtaatgga ctcggcgtca attttgagaa
      121 agccagtgtg ctcgacatcg gctgcggcac gggattttac accgatattc ttgagaggca
      181 gggagtgaag gattataccg gtgtagatat caccaaccat catttcgaaa aactcagaga
      241 gcgctttccg gactacagtt ttataaagaa agatatcact tcagaaaagc atgaaggtga
      301 ttacgacctg atcataatga tcgaggtgct ggaacatata gtcaatgacg ataagctcga
      361 ttttgccatg gagaatgtaa aaagatgcct gaaggaaaac ggggtcttcc ttatatcctc
      421 catctgggaa aaaggccgca aacatatgtt ctatgtgcgc aaatggacgc tggatgaaat
      481 taggaagcgc ttcagggact atcatttctc agagccgata ccattccgcg gcagctatat
      541 ggtcgccata cggaaatctc agcagtcaga taagtcgttt attaataaca agtagcacta
      601 ttgcggggtc atttctgcat cctttctttt cagaaaaatc ctgtctattc tttaaacatt
      661 ttcttaaatt gcaggtagaa caggtgagga tgagtatgaa ttctatcctc aatcaggacg
      721 gagtaagcat atgaagaaaa tgatagtatt acttttactt gctggcctgg ccgcatcgct
      781 ggttataacg tgcaatgaga ccagaattat caatactaat gaagatgatc tgattttgaa
      841 aaaagcagcg gcattttcaa ttattctccc cagccagatg gttgccggag aggatatgca
      901 gttccgcatt atactcgacg ccggttttcc ctgctataca tttgctcgtt acgacatcga
      961 ggaagtagga ggcgagattt ttattacacc tatggtatac caaagaggta cgtcctgcac
     1021 caattacact tcgcgaatcg acaggagcat gttctataga attgacgagc ccggaaaata
     1081 tctgttccat ttctatgaaa gtgagctgag ctacaagcat tatgagatag aagtcgagcc
     1141 cgcttcggga gaataacgag cttcgttccc gtttttcatt atccccctga gccttacaaa
     1201 tcttcccgag aagtattgaa tttttgtttc agataagcgc attttctttc gaaaatatat
     1261 atagagcgat ttatgtggaa ttcgggtgaa aaatatgagg gtgcttacct gggcacggaa
     1321 atggacgagc tctggtggaa gcggtataag aaagacaaga tatttgcccg gggaaacggc
     1381 aagttctggt ttgatgaaat gggcatatat tttcatcgct acctgacgcg ccggccgctt
     1441 tatatcccct ttaatcagat gcgctcgttc aagatcggcc agtggcacgc cggccgctgg
     1501 gccatgcaca taccgatcct caaaataatc tggtacaaag acaatcaaaa gttgtcatcg
     1561 ggcttcgcgc tcaccaaaaa gcgcagggag ctggcaaagc tcctcgaaat tttccagcaa
     1621 aaaacttcgg ctgtcttcga gaatgtctga gatattgttt cctcttgact accaaatttt
     1681 atttaatttg taagccgtct tacagctaat gccgtactct cggcacaatc ccgccataga
     1741 atcaacctca ttcaaatagt ccaatagaaa tctctctcgt tcgtccatga tatagacctc
     1801 cctccaaagc atgctacctt cttcgcatgc cataatatag agtgttaccc atcatcccgg
     1861 cctattttgt aacctatgtc tccggtctgt accatcactt aaacccacct ttcaggtggg
     1921 gtacaaatat tacaaaaaaa cggcaggccg tttatagcct gccgttttaa ttcaacgtga
     1981 tcaaatgtct tcgacatttg acgcaatgtc aaaactcaca gagttttgac ctacgatact
     2041 ttatatttat tcttccgctg cgatcttctc caggcgctgg atgtgacgac cgccttcgaa
     2101 atcggttgtt aggaatgtct ccactatttc aagcgcatct tcctccggca catacttcga
     2161 ggccaaagac aatacattgg catcattatg caaacgtgtc aacttagcca tttcagcgtt
     2221 gaggcataat cccgcacgaa tgcccttgac cttatttgcg gccataatca tgccgttgcc
     2281 ggtccagcag atatttatgc catagtcaac aataccatca gcgacagcat gagccacttt
     2341 caaaccgaaa tccggaaagt cgcaggattc ctcggcgtcg gtgccgaagt ccaaaacctc
     2401 atcatgccgt tcggtcagga agcgcttgat cttctccttg agcggataac ccttatgatc
     2461 cgctcccaat gctatcttca tatcctatgc cgtcactccg gcttctttgt acacaaaggt
     2521 cagccagccg taaggatcat tacccgcgac aacgacgtca aagaaagcgt tctggagctt
     2581 ctcggtaatc gggccgcgtt cgccagttcc gatcttgaca ccgtcaatcg aggaaatagg
     2641 ggtgatttcc gctgccgatc ccgtgaaaaa cacttcatca gcgagataca gagcttcacg
     2701 aggaatattc tcctctttga ccggaatagc catctc
//
LOCUS       DSEB01000298            3098 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003837, whole genome shotgun sequence.
ACCESSION   DSEB01000298 DSEB01000000
VERSION     DSEB01000298.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003837"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..230)
                     /locus_tag="ENO07_05380"
     CDS             complement(<1..230)
                     /locus_tag="ENO07_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HDS01436.1"
                     /translation="MMDRKTKNLFEARAKIIKALAHPARLFIVDQLSHGEKCVCELTG
                     MIGSDVSTVSKHLAVLKNSGIVLDEKRGTQVF"
     gene            complement(337..1371)
                     /locus_tag="ENO07_05385"
     CDS             complement(337..1371)
                     /locus_tag="ENO07_05385"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDS01437.1"
                     /translation="MKYIDLRSDTVTRPSDEMRRIIAQAEVGDDVFGDDPTVNKLQKK
                     VAGLFGKEAALYVPSGSMGNLVAIKTHTNPGEEAIFEEDCHSLNYEAGSMGAVAGLVS
                     HTFRGRHGVMTREQVEPYIKKKSLHTPPTTLIALENTHNHAGGTIYPLEEIKKLREMA
                     EEHELRMHLDGARIWNASVATGIPLDEYGRYFDSIQCCFSKGLGAPIGSIVVGDADFI
                     ERARRFRKMFGGGMRQVGIIASAAIYALDNNMSRMSEDHENAGFLAQNLAGIDGIDID
                     LDSVQTNIVIMNIASSGKQVPDVLSKLKEMGILAVQFGATKIRCVTHLNVNRTDIETA
                     IEKFKAVFAN"
     gene            complement(1439..2077)
                     /locus_tag="ENO07_05390"
     CDS             complement(1439..2077)
                     /locus_tag="ENO07_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01438.1"
                     /translation="MNQEDLLSDAPAKKEISYNEEAMRKAVHVFSIVIPVCYLFLPRS
                     IMLVVLLIASSVAVTIDLSRITGLALWSKFLVKVLGSMIRPKEKEGFTGASYILSTDF
                     LVILLFDKPVAIAAIAYIALGDTAAAMVGRRWGFHRYGDKSIEGSLAFFIVTAGAGIG
                     FHYLYPGALPVIAAVAGAAVATIVEAMTVRSDDNMTVPIVSGLFMQILIAFI"
     gene            complement(2083..>3098)
                     /locus_tag="ENO07_05395"
     CDS             complement(2083..>3098)
                     /locus_tag="ENO07_05395"
                     /inference="COORDINATES: protein motif:HMM:PF13145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01439.1"
                     /translation="FRVPQRSGDDYAPLDSNLKAFLHGELVSAKTRSEAARYFDSLVA
                     VSDVQYNMEFIEGGQEMDDDDWVVVMRESDTIYFPEYKKWRDNELKKDPRIRETMQFR
                     RELIGQIASTWMLIFEAREQGYHESDEYVKAKNEFLYQEKLNKLMSQRHGDGYEPTDS
                     QIAAYYEAHKEEFTEDEMISIQQVILNDLATANEVKARLDNGANFYQTALEYQPGEVE
                     EIRDMAINLGWISREEISPAFFEKIYALEPGQISEPIRTDWGYHIVHVTGKKGIKPLE
                     TVRIEIRKKLIQNHKDSLEAEWAERMLEGVEVKVDNELFEEFIFHKEWLPKPDFSKLA
                     PRY"
BASE COUNT          752 a          835 c          710 g          801 t
ORIGIN      
        1 tagaaaacct gggtgccgcg tttctcgtct aggacaattc cgctattctt gagaacagca
       61 agatgtttcg agactgtgga gacatcggaa ccaatcatcc cggtcagctc gcagacacat
      121 ttctctccat gtgaaagctg gtcgacaatg aaaagccggg ctggatgtgc cagggccttg
      181 ataatcttgg ccctggcttc aaataggttt tttgtttttc tgtccatcat aaaaaaatac
      241 gaaagcaaat tattggttat ttggcaaaat agccaaataa tataaaaaag caataatatt
      301 ttcatgaatt tatggccggg tagaagaaat caggcactaa tttgcaaaaa cagccttgaa
      361 tttctcaatc gccgtctcaa tatccgtgcg gttgacattt agatgagtga cacagcggat
      421 cttggttgcc ccaaactgca ccgccagtat tcccatctct ttcaatttgg aaagtacatc
      481 cggaacttgc tttcccgaag aagcgatatt catgatcact atattggtct gcacactgtc
      541 gagatcaata tcgattccgt cgatccccgc cagattttgc gccagaaatc cggcgttttc
      601 atgatcctcg gacatgcggg acatattgtt gtccaatgca taaatagcgg cggaagctat
      661 tatccccacc tgcctcattc ctccaccgaa catcttgcgg aaacgtcggg ccctctctat
      721 aaaatcggca tccccgacca cgatcgatcc gattggcgcc cccagaccct tggagaagca
      781 gcattgaatt gaatcaaaat aacggccgta ttcgtccaga ggaatcccgg tggctactga
      841 ggcgttccag atccgcgcgc cgtccagatg catccggagc tcatgctcct cggccatctc
      901 ccgcagcttc ttgatctcct ccaggggata gatcgtaccg ccggcatgat tatgtgtatt
      961 ttccagcgcg atcagcgtgg taggcggagt atgcagggac ttttttttga tatatggttc
     1021 cacctgttca cgggtcatga caccgtgtct gccccggaat gtatgcgaca caagcccggc
     1081 caccgcgccc atggaaccgg cttcatagtt cagcgaatgg cagtcctctt caaaaatagc
     1141 ctcctcgccg ggattggtat gagtttttat tgcaaccaga tttcccatcg accccgaggg
     1201 cacatacagg gccgcttctt tgccgaataa tccagccact tttttctgca atttattgac
     1261 tgtgggatca tcgccgaaaa catcatcgcc gacttcagcc tgtgcaatta tccggcgcat
     1321 ttcatccgag ggacgggtga ctgtgtcgga gcgcagatcg atatatttca taacagactc
     1381 ctgtgttctc ctgaaaattc agtctattaa ggtagattga gggagcctgg ctgtcaagtc
     1441 aaataaaggc tattagaatc tgcataaaga ggccggatac tatgggcacg gtcatgttgt
     1501 cgtcggagcg gactgtcatc gcctccacta tcgtggctac cgcagctccg gccacggcgg
     1561 cgataacggg cagagctccg ggatagaggt aatgaaatcc aatcccggca cccgcggtca
     1621 cgataaagaa ggccagagag ccctctattg atttgtctcc gtacctgtga aaaccccagc
     1681 gtcgccccac catggcggcg gctgtgtccc ccagggcgat ataggcgatg gccgcaatcg
     1741 ccaccggctt atcgaacagg agtatgacca gaaaatcggt actgaggata tagctcgccc
     1801 cggtgaagcc ctctttctcc ttgggacgaa tcatggatcc aagaacctta accagaaatt
     1861 ttgaccatag agccaagccg gtaatccggg aaagatcgat agtaacggca acagaactcg
     1921 caatcaaaag caccacgagc atgatggatc ggggaagaaa aaggtagcag accggaatca
     1981 cgatgctgaa gacatgtacc gccttccgca tcgcctcttc attgtacgaa atctccttct
     2041 tagcaggagc atctgagagg agatcttcct ggttcatttg aattaatacc ttggggccag
     2101 cttggagaaa tccggcttag gcagccattc cttgtgaaag atgaactctt caaagagctc
     2161 gttgtccacc ttgacctcga ctccttccag catgcgttcg gcccattctg cttccaggct
     2221 gtccttgtgg ttctgtatca gtttttttct aatttcaatc cgaacagttt ccagcggctt
     2281 aatccccttt ttgccggtga cgtgcacaat atgatagccc cagtcagtcc ttatcggttc
     2341 ggagatctga cccggctcca gcgcataaat tttttcaaaa aatgcgggtg agatttcctc
     2401 cctcgaaatc catcccaggt ttatcgccat gtccctgatc tcctcaacct cgccgggctg
     2461 atactccaga gcggtttgat aaaaatttgc gccgttatcg agcctggctt taacctcgtt
     2521 ggccgttgcc agatcattca atattacctg ctgaatactg atcatttcgt cctcggtgaa
     2581 ctcctcctta tgcgcctcgt aataagccgc aatctgtgaa tcggttggct catagccgtc
     2641 tccgtgccgc tgggacatca acttattcag tttttcctgg tagagaaatt cattcttggc
     2701 cttaacatat tcatccgatt cgtgatagcc ctgctcccgg gcctcaaata tcaacatcca
     2761 ggtgctggca atctggccga tcagttcgcg cctgaattgc atagtctccc tgatcctggg
     2821 atcttttttc agctcattat cccgccattt tttatactca ggaaaataga tggtgtcgct
     2881 ctcgcgcatg acaactaccc agtcgtcatc atccatttcc tggccgcctt ctatgaattc
     2941 catgttgtac tgtacgtcag agacagcaac cagactgtca aaatatctcg ccgcctccga
     3001 ccgggtcttg gcggaaacca gttcgccatg cagaaatgcc ttcaagtttg aatcgagcgg
     3061 ggcataatca tcaccgcttc tctggggcac tctaaaga
//
LOCUS       DSEB01000299            1797 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1012818, whole genome shotgun sequence.
ACCESSION   DSEB01000299 DSEB01000000
VERSION     DSEB01000299.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1012818"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            118..1191
                     /locus_tag="ENO07_05400"
     CDS             118..1191
                     /locus_tag="ENO07_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01440.1"
                     /translation="MNGRNSLNISEKSIIALSGGLDSRAVLGILKAAGVGMLAASSIN
                     YSRSNLFDVNIAEKVAAVQDVEFIRIQLENPKFGDLEKIAEIKPGLNNISMANALGLY
                     TNLLDRFPYGADFYTGDGGASMKTPYRSQGGIKSKDQLLQQIFNTPAVFDDDEMKRLL
                     GLGISDLVDDFEGEVSSYPPESWREKYLYYTVFDYLTNFGYEGEDRSRYFFWTQTPFE
                     AIPFYINALRIPDRFKSGLKLMNRFLKLCDPALLNVVYADYGAKPESSRIRTKLLVRR
                     FMKRHPALLRRMRRVVFPRTYRPKPIEDHLLDYALGSLRHAGFLSNIIDSDYLDKLLR
                     RPISRSRFESLLSLILACRKMHG"
     gene            1327..>1797
                     /locus_tag="ENO07_05405"
     CDS             1327..>1797
                     /locus_tag="ENO07_05405"
                     /inference="COORDINATES: protein motif:HMM:PF13668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01441.1"
                     /translation="MQDVFDFAMQMEQDGRDYYEEMASKTKIPALKKILTGLADDEKR
                     HYQIFKNLKEQHSDQVSQLSEKSTTIMSDARNIFEQLSGDEVEVDTAGDAIEVWEHAQ
                     EIEKKSEDFYREKAGVVPDKTARDLLNKIADEEHKHWALIESVMQFLHRPDRWLE"
BASE COUNT          517 a          377 c          453 g          450 t
ORIGIN      
        1 gatacgagaa ctttctcaga gcccatcaga taccatcaat gggatattaa aacagtgcat
       61 gataaatctt caatagaaga gtctgctgag agactattat ttcaggcaaa ggatgctgtg
      121 aatggccgca attccttaaa catatctgaa aaatcaatta tcgctttatc cggcggcctc
      181 gactcccggg ctgtgcttgg gatattgaag gcggcgggtg tgggaatgct ggccgcaagt
      241 tcgatcaatt acagccgcag caatctattc gatgtcaata ttgcggaaaa agtagcggcc
      301 gtccaggatg tggaatttat cagaatacag ctggagaatc cgaaatttgg tgatttggaa
      361 aagattgcgg aaatcaaacc gggattgaac aacatttcga tggccaatgc tctggggcta
      421 tacacaaact tgttggacag gtttccgtat ggcgctgatt tttataccgg tgacggtggc
      481 gcttccatga aaacgccata tagaagccag ggcggaatta aatcgaaaga tcagttgctt
      541 cagcagatat tcaatactcc tgcagttttt gatgatgatg aaatgaagag gctgcttggc
      601 ctggggatct cagatttagt tgatgatttt gaaggagaag tatcaagcta cccgcctgag
      661 agctggagag aaaaatacct ttattatacg gtcttcgatt acctcacaaa cttcggatac
      721 gagggcgagg atcgcagccg ttatttcttc tggacccaga cgccctttga agcgatcccg
      781 ttttatatca acgccctcag aattccggac cgattcaaat ccggactcaa acttatgaat
      841 cggtttttga agttgtgtga tcccgcactt ttgaatgtgg tatatgccga ttacggagcc
      901 aaacctgaat catcccgtat acggacaaaa ctgctggtga gaagatttat gaaaaggcat
      961 ccggcgcttt tgcgaagaat gcggagagtt gtatttccgc ggacatacag gccaaagcca
     1021 atcgaagacc atctgctcga ttatgcattg ggctcattga ggcatgccgg ttttctatcc
     1081 aatattattg actctgacta cctggataag ctcttacgga gacccatcag caggtcccgg
     1141 tttgaaagcc tgctgtcgct gattttggcc tgcaggaaga tgcatggatg aaacataaaa
     1201 ctgaccacat caatcctctt gcgaagcagg tgcattttta ttagattaag agcctttggc
     1261 agaacaaatt gccgacattt gtgttgcttg attattgaag ataattaagt agaaaggaaa
     1321 tagaaaatgc aggacgtatt tgatttcgcc atgcagatgg agcaggatgg ccgagattat
     1381 tacgaggaga tggcctccaa gactaaaatc ccggctctga agaagattct tacgggcctg
     1441 gccgatgatg aaaagcgtca ttaccagata ttcaagaatc ttaaagagca gcactcggat
     1501 caggtctcgc agctttcgga aaagtcgacc accatcatga gcgacgccag gaatattttt
     1561 gagcagttat ccggagacga agttgaggtt gatactgccg gtgacgctat cgaggtctgg
     1621 gagcatgccc aggaaatcga gaaaaagagc gaagatttct atcgcgaaaa ggccggtgtc
     1681 gttcccgata agaccgccag ggacctgctg aacaaaatag cagacgagga acataagcac
     1741 tgggccctga tcgaatctgt catgcagttt ctgcatcgac ccgacagatg gctcgaa
//
LOCUS       DSEB01000300            2282 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1007544, whole genome shotgun sequence.
ACCESSION   DSEB01000300 DSEB01000000
VERSION     DSEB01000300.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1007544"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..637)
                     /locus_tag="ENO07_05410"
     CDS             complement(<1..637)
                     /locus_tag="ENO07_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008629752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HDS01442.1"
                     /translation="MTRYMGRLTILGILLVVFLTMNCDSNPAESEIILELVWQDEFEG
                     PAGQLPDPAKWGYDIGTDWGNAQLEYDTDRPENVSLDGDGNLAITAREESYMGQSYTS
                     ARIVTRDKFEPTYGRIEARIKLPTGQGIWPAFWMLGNDIETVGWPQCGEIDIMEYRGQ
                     EPQIIHGSLHGPGYFGAEALTSRFYLENGRFDTDFHVFAVEWRAGEIRWYVD"
     gene            complement(618..1883)
                     /locus_tag="ENO07_05415"
     CDS             complement(618..1883)
                     /locus_tag="ENO07_05415"
                     /inference="COORDINATES: protein motif:HMM:PF00480.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HDS01443.1"
                     /translation="MKVKLTLSNSSGKRAVLSRERPLANSVLKLIWEKKKTSRAEIAR
                     LAGLSRSTVSEIIDSILTTGLIAEVGAGKSRGGRRPIVLEFQDEARWILGVDLGATHV
                     SVALTDLRGRVLSWQEKKHPVRTDPRGTRELVISLCDECLKEWGNGTRRLNSIGVAVP
                     SPVDPLNPRLLSEVVIPAWQGESGLEQLQIRYGVPVYVDNDANLGALAEHWWGAGKGI
                     NDFVYIKIAHGIGAGYILNGEIYRGAGGVAGEMGHLPIDPNGPLCVCGLRGCLATFVG
                     KPAIAERTKKLLKKYPGSQLKGQDHTLDAITKAALDGDALALKIVSEVAEYLGVAISG
                     WFNLMNPNLAILGGGFAGLGELLLEPLRAKVRNSTLVSKAAVDIKTSDLGPRAVAIGA
                     ATLALEAMFADPIEVHTRANGRINDSVHG"
BASE COUNT          577 a          625 c          488 g          592 t
ORIGIN      
        1 catcaacata ccatctgatt tcgccggctc tccattccac agcgaaaaca tggaaatctg
       61 tatcgaagcg gccattttcc agataaaatc tggaagttaa tgcttccgct ccgaaatatc
      121 ccggaccgtg caaacttcca tgaataattt gcggttcctg gccgcggtat tccataatat
      181 ctatttcgcc gcattgcggc cagccaacag tctcgatatc gttgccgagc atccagaagg
      241 cgggccaaat tccctggccg gttggaagtt taatgcgggc ttcaattctg ccataagtcg
      301 gttcgaattt atcgcgggtt acaattcgtg ccgaggtata actctgcccc atgtatgatt
      361 cttcacgagc tgtgatggcc agatttccgt cgccatccag cgagacgttt tccggccggt
      421 cggtatcata ttcaagctgg gcattacccc agtcggtacc aatatcatag ccccatttgg
      481 caggatcggg taattgaccg gccggcccct caaattcatc ctgccagact aattccaaga
      541 ttatctcgga ttcagccgga ttgctgtcgc aattcatggt cagaaacact accaaaagta
      601 tgccaagaat tgtcaatcta cccatgtacc gagtcattta ttctcccgtt tgcccgtgta
      661 tgtacctcga tcggatctgc aaacattgcc tccagagcca aagtggcggc gccaatggca
      721 accgcgcggg gaccaagatc acttgtttta atatcaactg cagctttgct taccagtgtg
      781 ctgttacgta ctttggctcg caaaggttcg agtagaagct cgcccaatcc tgcaaatcct
      841 ccacctaata tggcaagatt cggattcatt aaattaaacc agccagagat agcgacaccc
      901 aaatattcag caacctcgct tacaatcttt aaagccagtg catctccatc gagagcggct
      961 tttgtgatgg catccaaagt gtgatcctgc cccttgagct gactgccggg atatttcttt
     1021 aacagctttt tggttcgctc cgcaattgcc ggttttccca caaaagttgc caggcatcct
     1081 ctcaaaccac agacgcaaag tgggccgttg ggatcaattg gaagatgtcc catctctccg
     1141 gccacaccgc cggccccgcg gtatatttct ccgtttaaaa tatatccggc acctattccg
     1201 tgagctattt ttatatagac aaaatcatta atgcctttgc ctgcacccca ccagtgttct
     1261 gcaagagcgc caagattggc gtcgttatcg acatagaccg ggacgccata tcgtatttga
     1321 agctgctcca gtccgctctc accctgccag gcgggtatta caacctcaga cagcaatctg
     1381 ggattcaaag gatccaccgg actgggaacc gcgacaccga tactgttcaa tcgccttgtg
     1441 ccatttcccc attctttgag acattcatcg cataaactga tgacaagctc gcgcgtacct
     1501 ctcgggtctg ttcttacagg atgtttcttt tcctgccagg agagtacacg ccctcgcagg
     1561 tcggtgaggg ccaccgaaac atgggtggcg cccaaatcaa caccaaggat ccagcgagcc
     1621 tcatcctgaa attctaatac tatcggacgc cgaccacctc ttgatttccc ggccccaact
     1681 tccgcaatca gaccggtcgt tagaatactg tcgataatct ccgagaccgt tgaacgggac
     1741 aagcctgcca ggcgcgcgat ttcagctctt gaagttttct tcttttccca gattaatttc
     1801 aagaccgaat tagcaagggg ccgttctcgt gaaagaacgg ccctttttcc agaagaatta
     1861 ctcaatgtta gcttcacttt catcgcttat acgatatctt tacaatcaca atctacttat
     1921 gaaaatagac attgtcaaca aagactgtac cataagtgcc tgaaagaatc agctgagcaa
     1981 aatgttcatt ggtcgtcatg ccagcagtgg caaaatcggc taacggaata tccaagctga
     2041 tccaggtgcc agtgctcatc gtagtattat caaaaaccaa ctctccctcg gtatcatcac
     2101 cagctgcatc ccagactccg ttgggaccat agtcaaccaa tttgatcttg aactctgaag
     2161 atccatcagt ggcaaccggg gtccagacat caatatggaa atgggtcatc gtgcttatgt
     2221 tttgagtccc tgtcgttgta tactcggcaa tcgagaaaac cagattagtg tatttcttga
     2281 gg
//
LOCUS       DSEB01000301            4965 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001340, whole genome shotgun sequence.
ACCESSION   DSEB01000301 DSEB01000000
VERSION     DSEB01000301.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001340"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            1003..2544
                     /locus_tag="ENO07_05420"
     CDS             1003..2544
                     /locus_tag="ENO07_05420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HDS01444.1"
                     /translation="MWRSGLRDNNSSHYLRGIIVFFISAVIIIGLGIVSTQFLVPGKA
                     SYNWDSGGTSVLPVASTREYPVTEEGESPFVAVVEKTRDAVVNIYAEGENPNARNMDP
                     MWRRFFGLPPRVSSYGSGFVIRPDGYILTNNHVVQGGDKITVTLSDDRTFDAELIGND
                     PQTDLAVVKIDVRDSLPFIELGNSDDIRVGDWVVAIGNPFPEQGLDRTVTVGVVSAKS
                     RRDLRFGSDTPEYQDYIQTDASINPGNSGGPLVNLRGEAIGINSAIASPNRGSVGIGF
                     AIPSNLARIVASDLIEEGKVSRGWLGVMLSELTQDQREASGVDADAGVRIADVIPGSP
                     ADAGGLEPNDIVLDFNGKEVPDLYTFRLMVAEREMGEKVDLKINRDGKEKNISIILGD
                     RDMSLAQSQGQMFPDQDRGDDDPQNTASWLGMSVETATPILARQYEVDYHQGVIVTDV
                     EPGSPAYRKNIVPGTIITKIDFRDVRSKEDFVRISNELQDREKAIAFYIFDLNGNIGY
                     VALKP"
     gene            2606..3460
                     /locus_tag="ENO07_05425"
     CDS             2606..3460
                     /locus_tag="ENO07_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDS01445.1"
                     /translation="MPRQSRKYRDEDRSLDLYLQEIGETPLLTPDEEVSLAKRIHQGD
                     ELALEKLTKANLRFVVSVAKQYQNQGMGLPDLINEGNLGLMKAAQRFDETRGFKFISY
                     AVWWIRQAILQALAEQSRIVRLPLNRVGTLHKIGKISSSLEQEYGREPSADEIANELA
                     LTASEVTDTLKISNTHLSLDAPFSSSEDNSLIDVLEDEYQPSPDEALLSQSLMTEIEK
                     ALHTLTPREAEVINLYFGLNHEKALTLEEIGARFSLTRERVRQIKEKAIRRLRHASRS
                     RSLRAYLN"
     gene            3664..4737
                     /gene="hemE"
                     /locus_tag="ENO07_05430"
     CDS             3664..4737
                     /gene="hemE"
                     /locus_tag="ENO07_05430"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:PF01208.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDS01446.1"
                     /translation="MRDYNNIQNEGLSRVDSMSKKELFLANLEGRWTARTPVWMMRQA
                     GRYMQEYRNVRQRHSFEEICKTPEIAADVTCTPVTLFDLDAAIIFSDILFVVEPFGFK
                     LTYEPGPAIDPSLSSRDQAGRFRPYDPVDHLSFVGDAIVDAKRCLGPDIPMIGFCGAP
                     FTIFCFLCGVQAARGFFKPYKYMMNYTEETREILQVISEVSLEYLKMQADAGADYVQL
                     FDTFAGELSAEEFEIWSKPYLDYIINGLNESGIPSGIFIRNSNHLIDSISSMNMNSFC
                     VDWKGDLRSISNRLNPKILQGNLNPNLMLGPKEKVIDKTLRILESMRDYPGYIFNLGH
                     GVLPDTPPENVRAVVQTVHAFER"
     gene            4742..>4965
                     /locus_tag="ENO07_05435"
     CDS             4742..>4965
                     /locus_tag="ENO07_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDS01447.1"
                     /translation="MADNSNLDKLLKKYDRPGPRYTSYPMVPVWKSDFGHEEYVRALE
                     NSSKKPDEPLSIYLHIPFCRQRCWFCGCNTT"
BASE COUNT         1314 a         1125 c         1278 g         1248 t
ORIGIN      
        1 cgactatata gataatatca tatattccac acttcccggt attggacgag atctggacag
       61 gctgaaaatt gactatgcca ttcccgaatt gacaaatccg ggcagcctgg acaagtatga
      121 gacactgatt atcccggttt ccgagtatat ggatgcctcg gagcaggagc tgataataga
      181 acacataaaa cagggaaaga atgtaatatt tgcagggctg gtgcccagat atgattccaa
      241 cggcaacacc tgcaacatct tatcttcata tttgaagctc aggtcaacct cctcttttga
      301 ggtggcggac gtcaaattta acggccagaa ttttccggct ctgctttacg gcaccctgag
      361 atccagcgat tcacaggcga agacgatagc caaggccgga aagagcgtgg ttgcggtcag
      421 atccagccgc ttcaagggcc gggttatatt cgtcagtttt gatttatcga cccagtatta
      481 tcacaccaaa atggcctttt tagaggagtt gctggcggac tgcaagatcg agcccttcat
      541 ctacacctca aatccggatg tgcgggccat aatcagaacg gacaagaatc gcgccctttt
      601 atatctctta tattcgcgtc cgcaattgcc tttcaagaag atcgagcgcc atcctctgca
      661 ggttgcggtt caggcggaca tgaagaaggt gggcatacgt tcggccaaac tgggtatggt
      721 ggagctgttt tcgggtgaga agaagctcat aacctctcgt aatctggctt ccggactgat
      781 aatcgaattg cgcagcctgg acagccatgt ctggctcata gcgccggcac aaaaatcttc
      841 gtcaaagaca caaaaaaatc aataaaataa ccagttatat gagtatataa ttggatcggc
      901 aaaaaaaagg gaacaattgc ggcattttct ggtagtagag aatgtataat aaaaaagtat
      961 tgacatataa aaatttagca gtatattact tgtttccaaa ctatgtggag gagcggcttg
     1021 cgggacaata atagcagtca ctatctcagg ggcatcattg tcttttttat ttcagctgta
     1081 ataatcatcg gtctggggat tgtgtccaca cagtttctgg tgccgggaaa agccagctat
     1141 aactgggatt caggcgggac atccgtcctc ccggtagcat cgacaaggga ataccctgta
     1201 accgaggagg gtgaatcacc ctttgtggcg gtggtggaaa agacccgtga cgccgtggtg
     1261 aacatatatg ccgaggggga gaaccccaat gccaggaata tggatccgat gtggaggcgc
     1321 tttttcggtc ttcccccgcg tgtttcttcc tacggctccg gtttcgttat caggcccgac
     1381 ggctacattc tcaccaataa tcatgtggtc cagggcggag acaaaatcac tgtcacatta
     1441 tcggacgaca ggacattcga tgctgaatta ataggcaatg atccgcagac agatctggcg
     1501 gttgtcaaga tcgatgtccg cgacagcctg ccatttatag aattgggcaa ttccgatgat
     1561 attcgagtag gggactgggt ggttgcaatc ggtaatccct tccctgagca ggggctcgat
     1621 cggactgtta cggtcggcgt ggtttcggcc aagagccgcc gtgatctgag gttcggttcc
     1681 gacacgcccg aatatcagga ttacattcaa accgatgcct caatcaaccc gggcaattcc
     1741 ggtggccctc tggtaaatct gagaggcgag gccatcggta tcaattcggc cattgccagc
     1801 ccgaaccgcg gttcggttgg aatcggcttt gccataccct cgaacctggc gcgaattgtc
     1861 gcctccgatc tgatagagga gggcaaggtc tcaaggggct ggctgggtgt gatgctgtcc
     1921 gaattgacac aggatcagcg tgaggcctcc ggggtggatg cagatgcggg agtcaggatt
     1981 gcagatgtta tccccggttc accggctgat gccggcggac tcgagccgaa tgacatcgtc
     2041 cttgatttta acgggaagga agtacccgat ctgtacacct tcaggttgat ggtggcggag
     2101 agggaaatgg gagaaaaggt agatttaaaa attaatcgag acggcaaaga gaagaacatc
     2161 agtattattc tgggcgacag ggatatgagc cttgctcagt ctcaagggca gatgttcccc
     2221 gaccaggatc gcggggatga cgatccgcag aacactgctt catggctggg tatgagtgtg
     2281 gaaaccgcaa ctccgatcct ggctcgccaa tatgaagtcg attatcatca gggggtaata
     2341 gtaaccgatg tcgagccggg ttcaccggct tacagaaaaa atatcgttcc tggaacaatt
     2401 ataaccaaaa ttgattttag agatgtccgt tcaaaggagg attttgtacg tatatctaat
     2461 gagcttcagg atcgggagaa agcaattgct ttctatattt ttgacttaaa cgggaatatc
     2521 ggttacgtgg cattaaagcc gtaaatgact caaatatcac aataaatcat ttttaacaaa
     2581 aatcaatagg aggaaggtag cacttttgcc aaggcagtca agaaagtatc gcgatgagga
     2641 caggtcatta gacctctatc ttcaggagat tggcgagacc ccgcttctta ctcctgatga
     2701 agaggtttca cttgccaaac gcattcacca gggggatgag ctggctctgg aaaaacttac
     2761 caaggccaac ctgaggtttg ttgtttctgt tgccaagcag tatcagaatc aggggatggg
     2821 ccttcctgac cttattaatg aaggtaatct tggactgatg aaagcagccc agcgttttga
     2881 tgaaacccgg ggcttcaagt ttatctcgta cgccgtctgg tggatcaggc aggcaatcct
     2941 gcaggcactg gcggagcagt cccgtattgt acgcctgccc ctgaacaggg tgggaacctt
     3001 gcacaagatt ggcaagattt cctcctctct ggagcaggag tatggacgcg aaccgtcagc
     3061 cgatgagatt gccaatgagc tggcgctcac agcctcggaa gttaccgata cgcttaagat
     3121 ctccaacacg cacttatcat tggatgcgcc cttttcctca tcggaagaca atagtctgat
     3181 cgatgtgctg gaggatgaat atcagccctc gcccgacgag gcgctcctca gccagtcgct
     3241 catgactgaa attgaaaaag cccttcatac cttaacacca agggaagcgg aagtcatcaa
     3301 tctttatttt ggccttaacc atgagaaggc attaacattg gaagaaattg gggcccgctt
     3361 ttcgttaacg cgtgagaggg ttagacagat taaagagaaa gcaatcaggc gtttaagaca
     3421 tgcctcacgt tcgcgctctt taagagccta tcttaactaa gaaaattgaa agttcctatt
     3481 gtaaagagaa agcgctgtca gcgctttctc tttttttgta gttagaaacg ataaactctc
     3541 actatattca cagatgtcat attacggcgc tgatttatga aactattatc ccccgataca
     3601 ggtttaaaaa aagaacggtt tatgtagatt tatggcgata aattttatat actggctgat
     3661 tcgatgcgag actataacaa catccaaaac gaaggtttat ccagagtgga ttcgatgagc
     3721 aaaaaggagc tgtttcttgc caacctggaa ggtcgctgga ccgccaggac gccggtatgg
     3781 atgatgcggc aggccggccg atatatgcag gaataccgca atgtgcgcca gaggcattcc
     3841 ttcgaggaaa tctgcaagac gcccgagatc gctgccgatg ttacctgtac cccggttacc
     3901 ctgttcgatc tggatgcggc gattattttc tccgatatcc tgtttgtggt cgagccgttc
     3961 ggatttaaac ttacttatga accgggaccg gctattgatc cctctctctc gtccagggat
     4021 caggctggca ggtttagacc gtatgatcct gtcgatcatc tcagttttgt cggtgatgct
     4081 attgtggatg cgaaacgctg cctgggtccg gatataccca tgatcggttt ttgcggggcg
     4141 ccatttacga tattctgctt tctgtgcggg gtccaggccg cccgtggatt ctttaaaccg
     4201 tataaataca tgatgaacta tactgaagag accagggaga ttcttcaggt tatatccgag
     4261 gtcagcctcg agtatttgaa aatgcaggcc gatgccgggg cagattatgt gcagctgttc
     4321 gatacttttg cgggtgaatt atctgctgag gaattcgaaa tctggtccaa accatatctg
     4381 gactatatca tcaatggcct taacgagtcc ggcattccca gcggcatttt catacgaaat
     4441 tcgaaccatc ttatcgatag tatctccagc atgaatatga acagcttctg tgtggactgg
     4501 aaaggcgacc tgagaagcat ctcgaatcgg ctcaatccaa agattctgca gggcaatcta
     4561 aatcccaacc ttatgcttgg gcccaaagaa aaggtaatcg ataagacttt gcggatattg
     4621 gagagtatga gagattatcc cggctatatc ttcaatctgg ggcatggagt tcttcccgat
     4681 actccccctg aaaatgtcag ggcagttgtc cagacagtgc acgcattcga aaggtgatga
     4741 agtggctgat aactcaaatc ttgataaatt gttgaaaaag tacgaccgtc ccggaccgcg
     4801 ctatacgagt tatcccatgg ttccggtttg gaagagcgat ttcgggcatg aagaatatgt
     4861 ccgtgccctg gagaacagct ctaagaagcc cgatgagccc ttatcgatct atttgcatat
     4921 accgttctgc cgtcagagat gctggttctg cggatgcaac acgac
//
LOCUS       DSEB01000302            4581 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001610, whole genome shotgun sequence.
ACCESSION   DSEB01000302 DSEB01000000
VERSION     DSEB01000302.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001610"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(18..893)
                     /locus_tag="ENO07_05440"
     CDS             complement(18..893)
                     /locus_tag="ENO07_05440"
                     /inference="COORDINATES: protein motif:HMM:PF00005.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDS01448.1"
                     /translation="MAAIEIKNLKKHFGPTRAVDGISFDVEKKEIFGFLGPNGAGKTT
                     TIRCMMDFIRPTDGSVSILGIDSRGAAVELKNKIGYLPGTVRLYDKWTGEDHIDYVRR
                     LRKCNDNARDMAKFLDLDISVKTRHLSLGNSQKLGLILALMGDPEILVLDEPTLGLDP
                     LFQDSVYELLKKANSEGKTIFMSSHYLGDVERICDRVEIIKEGKLVAAEGVRDLKQKR
                     IYKVQAFFAGSFDRNAFEIEGVEITSETSESLSMDVKGDINPLIARLGNFELRDLQIE
                     HASLKDIFLEFYEKR"
     gene            1502..2038
                     /locus_tag="ENO07_05445"
     CDS             1502..2038
                     /locus_tag="ENO07_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDS01449.1"
                     /translation="MPDDVYELWPELEWIEDEKLRHATADTWALALEKSSLDAKDLNE
                     IPFTLLIENCKVTFMQHKRAVVHLARDCAAQMQKFFKDNLPIDMDVVIAGAILADVGK
                     LLEYEKVGGRVVQSDYGKVLRHPFSGVGLAMQCGVPREVCHIIAAHAGEGDMVKRTPE
                     AYIVHHCDFMSFEPFKER"
     gene            2089..2325
                     /locus_tag="ENO07_05450"
     CDS             2089..2325
                     /locus_tag="ENO07_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01450.1"
                     /translation="MFWKNAVLDALRRYSERHNTNTVIRSKLIREEIGNIVKDTRSKG
                     RTPEQTLSRIMQELRDDGYVHFVDDDGTYEINNL"
     gene            complement(2541..3809)
                     /locus_tag="ENO07_05455"
     CDS             complement(2541..3809)
                     /locus_tag="ENO07_05455"
                     /inference="COORDINATES: protein motif:HMM:PF12686.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HDS01451.1"
                     /translation="MCDSSNLDNFWKFNINPFLNSFRLLYCHQNRRNISYFTRSFKSV
                     NRKFAFIDESGDNGLNFSKSGTHFLVTAVIVNESDYDAVEKGTLKLRSHYFRGGELKS
                     SKIGKNAKKRKDILKDISGLKVRVFTVAIDKRHLVSKGFKYKKSFYKYIHRILDSELI
                     KVFEELTISSDSYGDAEFMEGFQAYIKKHHPPDLFNNFNFKFEGSKSNTFLQLADFIC
                     GSIGRVYNSNDDDSDKSQYIEILKPVLILIYHWPPRYEKYINDLKDSGHIFNEIITDV
                     SCRIALNYISAWSSTTDQVKKYQVECLKYLLFNFYINPDKYISTKELIRHLSPLESDT
                     IKPTTFRLQVMAKLRDQGVLVASNERGFKIPTTEKDCLNFIETSNKTIAPMLTRIQRF
                     RNSILLATNGQFDVLGPSQFSYLKKVFDDI"
     gene            complement(3898..4128)
                     /locus_tag="ENO07_05460"
     CDS             complement(3898..4128)
                     /locus_tag="ENO07_05460"
                     /inference="COORDINATES: protein motif:HMM:PF04191.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HDS01452.1"
                     /translation="MIIYGPFKLSRHPMYLGMTAILPGEAILLGSLTAFIAPLGFFLV
                     MQAVFIPLEERSMSQTFGEEYTLYRKKARMWF"
BASE COUNT         1225 a         1032 c         1026 g         1298 t
ORIGIN      
        1 caatatcaca gacataatca ccgcttttca taaaattcca ggaagatgtc tttcaatgat
       61 gcatgttcaa tctgcaaatc cctcaattca aaattaccga gtctggcgat aagaggattg
      121 atatcccctt tgacatccat tgaaaggctc tcggatgtct cgctggtgat ctcgaccccc
      181 tctatctcga atgcatttct gtcgaatgat cctgcaaaga aggcctggac cttgtatatc
      241 ctcttctgct tcaaatccct caccccttcc gccgccacca gtttgccttc cttgattatc
      301 tccacccgat cgcatatccg ctcaacgtcg cccagataat gcgaggacat aaaaatggtc
      361 ttgccctcgg aattggcctt cttgagaagt tcatagactg aatcttgaaa taacggatcg
      421 aggccgagtg tgggctcatc gagaaccaga atctccgggt cacccataag tgccaggatc
      481 aatcccagct tttgactatt tccgagagag aggtggcggg ttttgacgga aatatccaga
      541 tccagaaatt tagccatgtc cctggcattg tcattacatt ttcgcagcct gcggacatag
      601 tcgatatggt cctcacccgt ccatttatca taaagccgca cagtccccgg gagatagccg
      661 attttattct tgagctcgac cgcagccccc cgtgaatcta tacccagaat gctgacactg
      721 ccgtcagtgg gacggatgaa atccatcatg cagcgtatcg tggtggtctt acccgcgcca
      781 ttgggaccca gaaagccgaa gatttccttt ttctcgacat cgaacgaaat gccatcgaca
      841 gcccgtgtcg gcccgaaatg ctttttcagg tttttgatct cgatagcggc catgcagaaa
      901 ttataaaata ttcaaatcga tatggaaatc tttaaatttg atggggccgc gggtccgtgt
      961 caacctcata attcggatgg atggttccac ctggtcattc agggggggca tctggaccaa
     1021 gtcattccga ggagatcata atctcggatt atgatcgacg tgggaatctc ctccgctgtg
     1081 ctatcagact aaactaagag attgccacgc ccggctgcag ccaggctcgc aatgacgata
     1141 gggtggcacg ccctaattca tccgggcctg ggcagattat aatcaagcgc tcggccaacc
     1201 gcctatgagc aggccaccct ctatcatatc gctccgaaca ctccaatctg tagcgcaggg
     1261 tcttgtccgg ccctggccgg atgtgaccct gcgcatccat acggaggacc gcacctgctc
     1321 tgacatcggc cgtcgctcag tttcttgcct tgcctcgggc atggtgtgaa cccgcgcatc
     1381 tctgacacca ccacacactt tcatttgaca atccctttaa catcaataaa ttgatttccg
     1441 aatcacttta taccgggtgc cccagccgct ttagcggtgg gaaccataga aggagaaagt
     1501 tatgcctgat gatgtatatg aactctggcc ggagctggag tggatcgagg atgagaaact
     1561 gcgtcatgcc accgcggaca catgggcgct ggcgctggaa aaaagttccc tcgatgccaa
     1621 agacttaaat gagataccat tcacgctctt aatcgaaaac tgcaaggtga cctttatgca
     1681 gcacaaacgt gcggtggtgc atctcgcgcg cgactgtgcg gctcagatgc agaagttttt
     1741 caaggataac ctgccgatcg atatggatgt tgtgattgcc ggggcgatcc tggcggatgt
     1801 cggcaagctg ctggagtatg agaaggttgg aggacgggtg gtgcagtcgg attacggcaa
     1861 ggtgctgcgt catccctttt cgggtgtggg gctggcgatg cagtgcgggg tgcccaggga
     1921 ggtctgccat ataatcgcgg cgcatgccgg tgagggcgac atggtcaagc gcacccccga
     1981 ggcctacata gtccaccact gcgacttcat gagctttgag ccgtttaagg agaggtagga
     2041 tgggattgac ttaaatatat tattacgaag attaagcgag gagttaaaat gttctggaaa
     2101 aatgctgttc ttgatgcttt aaggcgctat tcagaaagac acaatacgaa tactgtaata
     2161 agaagcaagc taattagaga agagattgga aatattgtga aagatacaag gtcaaaaggc
     2221 agaacgcctg agcagacact cagtcgtatt atgcaggaat tgcgggatga cggttatgtg
     2281 cattttgttg atgatgatgg tacgtatgaa ataaacaatt tatgacatta acgaatatgc
     2341 ctaattggac ttaaacttaa cttctgcaag cttcttgagg tttccttgga ttcgattacc
     2401 gtgcaatgta ttataatctc tattagacta ggcaagcttt cctttttatg gtttgtataa
     2461 tccgaggact tttttgtgtc aggtaaaatc aaaaaattca gtactcaagt tcttttgcac
     2521 acagtagtta tggaaatagc ttagatgtcg tcaaagactt ttttgaggta gctgaattga
     2581 gatggtccaa gaacatcgaa ctgtccatta gttgccaaca gaatgctatt tctaaatctc
     2641 tgtatccttg ttaacatggg cgcaattgtc ttattgcttg tttcgataaa attaagacaa
     2701 tccttttctg tggttggtat tttgaagccc ctctcattac ttgctaccag aacaccttga
     2761 tccctcaatt ttgccatcac ttgtaatcgg aaggtcgttg gtttgatggt gtctgattca
     2821 agaggagata ggtgtctaat cagttctttg gtgcttatat atttatcggg attgatataa
     2881 aaattgaaca ataaatattt taagcattca acttgatatt ttttgacttg atccgtcgta
     2941 gaactccagg cactaatata gtttaatgca attctgcaag atacatccgt tatgatttcg
     3001 ttgaatatat gaccggaatc tttgagatca ttgatatatt tctcatatct tggcggccag
     3061 tgataaatta gaataagaac tggttttagt atctcaatat attgcgactt atccgaatcg
     3121 tcatcgttgg aattatatac tctcccgata cttccacaaa tgaaatccgc caattgaaga
     3181 aatgtgttgg atttactacc ttcaaacttg aagttaaagt tgttgaacag atcgggtgga
     3241 tgatgctttt ttatataagc ttggaaacct tccatgaact ccgcgtctcc atagctatcg
     3301 gacgaaatgg tgagttcttc gaaaactttg attagctctg aatctaaaat tctatggata
     3361 tacttataga atgacttctt atatttgaat cctttggaaa caagatgccg tttatcaatt
     3421 gctacagtaa agaccctgac ttttaatcca gaaatatctt ttagaatgtc ttttcttttt
     3481 tttgcattct tcccgatctt acttgacttt agttcgccgc ctctaaaata atgactccgc
     3541 aatttgagag tgcccttttc cacagcgtca taatccgatt cattcacaat aactgcggtg
     3601 actaaaaaat gtgtgcctga tttagaaaaa tttagaccat tgtctccaga ttcatctata
     3661 aatgcaaatt tacggttcac agatttaaaa ctccgagtaa agtatgaaat attgcgccta
     3721 ttttgatgac aatataataa acggaaggaa tttagaaaag gatttatatt aaatttccaa
     3781 aaattatcta gattagatga gtcgcaaatc cactagtttt cagatcttaa gaagcagtcg
     3841 ctatcagagc cgtcatcata ttcttaaagg ctataattca ccaagatgtt gttacgccta
     3901 aaaccacatc ctggcctttt ttcggtacag ggtgtattcc tcaccgaagg tctgggacat
     3961 ggaacgttcc tcgaggggga taaagaccgc ctgcatgacc agaaaaaatc ccaggggggc
     4021 tatgaacgca gtcagcgatc caagcaagat cgcctctccc ggaagaattg cggtcatacc
     4081 gaggtacatg ggatggcggc ttaatttgaa cgggccatag attatcatgg cgctgggctt
     4141 ctcatgtggt ttgactgccg tttcggccgg taacggacag ctaccggggt aagccgattt
     4201 actttagctt cctgacttcg atgaataatg tatcggattg gcagatcggc ctgcctttca
     4261 tctcatccag aaaatcatcc agccgcttca tccagtaatc cccgcgcccg gtcttgaagg
     4321 caaattcctc aaactggtcg aaatgatgct catatgtttc cggctcatcg cacttctcaa
     4381 tttcgatttc gaaatttaag cccttatgtt tcaaatagcc tttcagggtc tcgagtgaaa
     4441 tatatttaaa atgcgaatag ccggcctctc gctgcacctc caggaagaac tcctgggcca
     4501 tgatggcgtt gcgcttggct tcactgcggg gatcattcaa cggttcctca atcagaacat
     4561 agttcgagga cagcctgagc a
//
LOCUS       DSEB01000303            7098 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000625, whole genome shotgun sequence.
ACCESSION   DSEB01000303 DSEB01000000
VERSION     DSEB01000303.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7098)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7098)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7098
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000625"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..2652)
                     /locus_tag="ENO07_05465"
     CDS             complement(<1..2652)
                     /locus_tag="ENO07_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01453.1"
                     /translation="MSISITTTAMADEYLESLTENQKAAGFSAVNVYENALGNIMGAR
                     FISDKYGFIVDLLKIQSVPQGFFWVKTPPSSDMGESHTCEHLLLGKGTQGRYVSVLED
                     MSLGNSSAWTAQTNTVYHFNTVAGDEAFYNLFEAKLNALLNPDFTDEEIRREVCHVGV
                     NLDPRDSSLSLEEKGTVYTEMVSAFERPQRFLWKELGETLYGKNHPLGNVSGGYPDDI
                     RKMTPEDMWHFHEESYYLGNMGVIVAIPNDIKTEDFLTKVSAILERCQSFEKNSTNIG
                     MNNFDFPPPAALDMAAVTRVVPAPSQNPDDPGDLMAAWPADLEYDLFENMVLETFLST
                     FAGGPTSNLYDLFINSETRKIDLGANSVWGYVSSEMGHPIIFGLNGINSDYITEEMLD
                     SIRGMIIEEIRNVHNYNNGSEQLAKFNEYARGRVIERQKQLEKLLNTPPMFGFRRAGG
                     QWQDILTMLEMSDEFRKSVVLKNRIPKLQQLLSSDTNFWKEYIDRWNLLSAKPHVLAS
                     KPDPSMLTKFAEEKQKRISGYLAEFKKKYNVDSDEGAIAKFKEEFDRKTAELEAIAAN
                     DEMPEFIDNPPMTLDDQLDYEVMELDNGVEMVASTFENMTSNQIAIALRLDVIPESQM
                     VYVPFLPDILTDVGVVKDGQVIRYEEMRNRLREDVLNFGSFFEYGLDEGRVELVLRAS
                     GNNQQELKNGIEWMNAALYSPYLDTNNLSRMMDIVDQSLVSLRNRMKGREEDWVRSPA
                     NGYRYQTNPLIMSTNCFLTRTHHYQRLKWLLTDPGSEDDRVMLSSYLAELKEKGKGLD
                     RESLKNLIDNPPAFPSSEKCEKIIPQVLRALGNSLADVPDANLSEDWQYLLQETEIDL
                     MVEPSRAIEDMNNILAALRRADNARMYM"
     gene            complement(2822..3730)
                     /locus_tag="ENO07_05470"
     CDS             complement(2822..3730)
                     /locus_tag="ENO07_05470"
                     /inference="COORDINATES: protein motif:HMM:PF14219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HDS01454.1"
                     /translation="MALEFTCIRCGNKIIVKYIRKGEIAVCRSCGAKNVVPETAAGVA
                     ELDSRLEDLKVFNERPSPTLEDINPSRITPLDFSAKAAIFFVGLSIVLWAANGILDII
                     LIDTLKKLEAVTVIDLANRAVQLEKWGNTIFILSLGEFIASAITFFLWFYRAHKNLYR
                     ARVPFLKYASGWTIGGFFVPFLNLVRPFSMMKEIWRGSVIMSSIRNTVEMEYLETSKK
                     IGWWWGFFLSSRFFSSLSRRFLRMAEEPGEFINPAWLELISSILTVVSGIILILLVKE
                     ITSHQMKARERSVPGVEIPDQISSDT"
     gene            complement(3841..5352)
                     /locus_tag="ENO07_05475"
     CDS             complement(3841..5352)
                     /locus_tag="ENO07_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDS01455.1"
                     /translation="MSWEGIVSQLDLLPNYLGSHLLLTMLALALGIFICLPLGCLVTR
                     VKSLQWPVLSIAGILQTIPGIALLALMVPILGTIGFVPALVALTIYSFMPILRNTVTG
                     ILGVDPAVVEAARGIGMTNVQLLLKVELPLALPVIIAGIRTATVWVVGTATLATPVGA
                     TSLGNYIFSGLQTQNLSAITVGCVSAAVLAIVLDQLTRLIEVAASKRSLKIGIAAGAA
                     LIVLLIAGISPAISSNREGHKLGSVTIGSKPFTEQYILADLLSDIVIRAGYNADKRSG
                     MGSIILFEALSNNSIDCYVDYTGTIWTNVMKRQDIPSRESILQEMTDWLDDEHGILCL
                     GALGFENTYALAVKVDFAGEHNLETIVDLAQLSSQLVMGSDYEFFSRPEWESLESTYD
                     LNFKDLRTFDPSLMYGAIAGGNVEVISAYSTDGRIAAYNLKVLDDPHQAIPPYDAVLL
                     LSPEASQDMNLINQLKVLIGGITDSEMRQANKIVDLDKLPVDSAAEYLKENIR"
     gene            complement(5349..6122)
                     /locus_tag="ENO07_05480"
     CDS             complement(5349..6122)
                     /locus_tag="ENO07_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDS01456.1"
                     /translation="MISVKNLSKSYDRISYAVDDISFNVNPGEILILAGESGCGKTTT
                     LKMINRLIEPSSGEIIVNDVNILDLNPVELRRRIGYVFQEIGLFPHMTISENVGIVPK
                     LLGWSRANIKARVDELMELTGLDPDKYAQKMPSELSGGQKQRVGVARALAARPDVMLM
                     DEPFGALDPITRSGIQDEFKNLQTELKLTVIMVTHDMMEALILADHVAIMQEGKIVVM
                     GTPGELMSKAGDAYAERLLHAPRQQSEKLAEILKRGDKK"
     gene            complement(6214..6498)
                     /locus_tag="ENO07_05485"
                     /pseudo
     CDS             complement(6214..6498)
                     /locus_tag="ENO07_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561442.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            complement(6522..6594)
                     /locus_tag="ENO07_05490"
     tRNA            complement(6522..6594)
                     /locus_tag="ENO07_05490"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(6559..6561),aa:Asn,seq:gtt)
     gene            complement(6739..>7098)
                     /locus_tag="ENO07_05495"
     CDS             complement(6739..>7098)
                     /locus_tag="ENO07_05495"
                     /inference="COORDINATES: protein motif:HMM:PF01569.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDS01457.1"
                     /translation="DQASSGIIKPLVGRMRPCKTLEGLHVLIDCSSGKSFPSGHATNS
                     FGQAAIWAWRYPKYKWFFIISAAIIALSRIFVGVHYSFDVLAGAILGTICGLIVFIFW
                     TLAKRIAVANKALDKRA"
BASE COUNT         1812 a         1772 c         1610 g         1904 t
ORIGIN      
        1 catatacata cgggcgttat cggccctccg cagtgccgcc agtatattat tcatatcctc
       61 aattgccctg gagggctcca ccatcaggtc tatttcagtt tcttgcaaca gatattgcca
      121 gtcctcggaa aggttggcat caggaacatc agccagcgag ttacccagag cccttaaaac
      181 ctgtggaatt atcttttcgc atttttctga tgatgggaac gcaggcggat tatctataag
      241 atttttgagg ctctctctat ccaaaccttt acctttttcc ttgagctcag ccagatatga
      301 cgataacata acccggtcat cctcgctgcc gggatcggtc agaagccatt tcaggcgctg
      361 atagtggtgg gtcctggtca ggaagcaatt ggttgacata atcaggggat tggtctggta
      421 acgatagcca ttggccggag acctgaccca gtcctcctcg cgccctttca tcctgtttcg
      481 caatgacacc agtgattgat ccacaatatc catcatgcgg gagaggttgt ttgtatcgag
      541 atagggtgag tagagcgccg cattcatcca ctcgataccg tttttcaatt cctgctgatt
      601 gttgccggag gctctcagca ccagttcaac acggccctcg tccaggccat attcgaagaa
      661 agagccgaag ttgaggacat cttcacgcag acggttgcgc atttcctcat acctgatgac
      721 ctgaccatct ttgacaaccc cgacatcggt cagaatatcg ggcaggaaag gtacataaac
      781 catctgcgat tcaggaataa catcaagcct cagcgctatg gcgatctggt ttgaggtcat
      841 gttctcgaat gtcgaggcca ccatctcgac gccattatca agctccatga cttcatagtc
      901 aagttgatca tccagagtca tgggcggatt atcgataaac tccggcattt catcattggc
      961 ggcaatggcc tcgagttcgg ctgtcttcct gtcgaattcc tctttgaatt ttgctatggc
     1021 tccctcatca gaatctacat tgtatttctt cttgaattcc gccagatatc cggaaatacg
     1081 cttctgtttc tcttcagcga atttcgtcag catggaggga tccggtttgg atgccagcac
     1141 atgcggtttc gctgagagca gattccatct atcaatatat tctttccaga agtttgtatc
     1201 cgaggataga agttgctgaa gcttgggaat tctgttcttc aataccacag attttctgaa
     1261 ctcatcgctc atttcaagca tagtcaggat atcctgccat tgccctccag ccctccggaa
     1321 gccgaacatg gggggagtgt ttaagagctt ctcgagctgt ttctgacgtt cgattactcg
     1381 gccgcgggca tattcattaa atttggcaag ctgctctgaa ccattgttat aattgtggac
     1441 atttcgaatt tcctcaatga tcatgccccg gatactgtca agcatttctt ctgtgatgta
     1501 atcagagttt atcccgttta atccgaaaat aatcgggtgt cccatttccg aactgacata
     1561 gccccagacc gaatttgcac ccagatctat tttgcgggtc tctgaattga taaacaggtc
     1621 atagagattg gaggtcgggc ctcccgcgaa ggtcgataaa aatgtttcca gaaccatgtt
     1681 ctcaaaaagg tcatactcga gatctgccgg ccaggcggcc attaaatccc cgggatcatc
     1741 aggattctgg gagggcgccg gcaccactct ggtcaccgcc gccatatcaa gcgcggccgg
     1801 gggcgggaaa tcgaaattgt tcatcccgat gtttgtgctg ttcttttcaa aactctggca
     1861 tctttcgagg atagccgata cttttgtcag aaaatcctcc gttttgatgt cattcgggat
     1921 ggcgacaatc acgcccatat taccgagata gtatgattcc tcatgaaaat gccacatatc
     1981 ttcgggggtc atcttgcgga tatcatccgg atagccgccc gagacattgc caagcggatg
     2041 attcttgcca tataaagtct cgcccagttc tttccataga aaccgctggg gacgctcaaa
     2101 agcggagacc atctctgtat aaacagtacc tttctcttcg agcgacaagg agctgtcgcg
     2161 cggatcgaga ttgacgccca catggcagac ctcacggcgg atttcctcat cagtaaaatc
     2221 cggattcaaa agcgcattga gtttggcttc gaagaggtta taaaaggcct catcaccggc
     2281 tacagtgttg aaatgataga ccgtgttggt ctgagcggtc caggcgctgc tgttcccgag
     2341 ggacatatcc tccagcacgg aaacataacg cccctgggtg cctttaccca gaagcagatg
     2401 ttcgcaggta tgggactcgc ccatatcaga gctgggcgga gtcttgaccc agaaaaagcc
     2461 ctgcggaact gactgaattt tcaaaaggtc gactataaag ccgtatttgt cagaaatgaa
     2521 tcgggccccc attatattgc cgagagcatt ttcatagaca ttaacagcag aaaaaccggc
     2581 tgctttctgg ttctcggtca acgattcaag gtactcatcg gccatggccg tcgtggtgat
     2641 gctaatactc ataagcaggc aaagtcccgt caacaatgtt ttccttagca cgctctttcc
     2701 tttctatatt actattatct gcaaattgtt ctaagtaaaa taatacgaac cagtaaacta
     2761 aatgttctgg agttttcctg tcaattagaa aatggatatg gtgttggtgt gtctatttag
     2821 tctatgtatc tgaagatatt tgatccggta tttccacacc cggaacagat ctttctctgg
     2881 ccttcatctg gtgactggtg atctccttga ccaacaaaat caaaatgatc ccgctgacaa
     2941 cggtcagtat cgatgaaatc agctccagcc aggcaggatt tataaattca ccgggctcct
     3001 ccgccatacg aagaaatctc ctcgatagag agctgaaaaa tcgtgaagac agaaagaagc
     3061 cccaccacca cccgattttt ttgcttgttt cgagatattc catctccact gtgttcctga
     3121 tgctcgacat aataaccgat cccctccaga tttctttcat catggagaac ggacgcacga
     3181 ggttcaggaa aggtacaaaa aaaccgccga tagtccagcc ggaggcatat tttagaaatg
     3241 gtacccgggc tcgatagagg ttcttatggg cacggtagaa ccacaagaaa aaagtgatag
     3301 cgcttgcgat gaactcgccc agagagagga tgaagattgt attcccccat ttctccaatt
     3361 gaacagccct gtttgccaga tcgataacag ttacagcttc gagtttcttt aaagtatcaa
     3421 tcagtataat atccagtatt ccatttgctg cccagagtac aatcgacagt ccgacaaaga
     3481 agatagctgc tttcgcggag aaatccagag gcgttattcg ggagggattt atatcctcca
     3541 gagtcgggga gggacgttca ttaaaaactt tcaagtcctc caggcgagaa tctaattctg
     3601 ccactcccgc ggctgtttca gggacgacat tctttgctcc gcaagaccga caaacagcga
     3661 tctctccttt gcggatatat ttcactatta tcttatttcc gcaccttatg cacgtaaact
     3721 ctaaggccat gtcctgccat ccattataaa ctttgcatgc ggctttatct ataacagtgt
     3781 caaaggcatt ttacattata gaggactaca atgtcaagtt taaattgtct atcccgcaat
     3841 ttaccggata ttttctttca gatactctgc cgcgctgtcc acagggagct tatcgagatc
     3901 tactatctta ttagcctgtc tcatttcact atcagttatg ccgccaatca acactttaag
     3961 ctgattgatt agattcatat cttgggaggc ttccggcgaa agtaacagaa cggcatcata
     4021 gggtggaatt gcctgatgcg gatcatcgag taccttcaga ttataagcgg caatgcggcc
     4081 gtcagtagaa taggccgaaa ttacctcgac atttcctcct gcaatagcgc catacatcag
     4141 cgatggatcg aaagtacgca ggtctttgaa atttagatcg taagtgcttt caagtgattc
     4201 ccattcaggg cgtgaaaaga attcatagtc gctgcccatg acaagctgcg atgacaactg
     4261 tgcaagatct actatcgttt ccagattgtg ttcaccggca aagtcaactt ttacagccag
     4321 cgcgtaagta ttctcgaagc cgagggcccc caggcacagg atgccatgtt catcatccag
     4381 ccaatccgtc atttcctgaa gtatgctctc ccgggacggg atatcctgcc gcttcatgac
     4441 attggtccag atagtgccgg tgtagtccac atagcagtct atcgaattat tggataatgc
     4501 ttcaaataga ataatggagc ccattcccga acgtttatcg gcattatatc cggctctaat
     4561 gacaatatcg gatagcagat cggccagaat gtactgctcg gtaaacggct tggagccgat
     4621 tgtgactgaa cccagcttat gcccctctct atttgatgat atggcaggtg atattccggc
     4681 gattaaaagc actatcagcg ctgctcctgc tgcaatgcca atcttcaggc ttcgtttaga
     4741 tgccgctact tcgattaatc tggtgagttg gtccaggacg atcgctaaca ccgcagctga
     4801 aacacatccg actgtaattg ctgagagatt ctgggtctgc agaccgctga aaatataatt
     4861 tccgaggctt gtggcgccca caggtgtcgc cagggttgca gtgccgacca cccagacggt
     4921 ggcggtacgt attcctgcga taattaccgg gagggccaga ggcagttcga ctttgagcag
     4981 tagttgcaca tttgtcatgc cgattccacg ggccgcctcc accaccgccg gatcaactcc
     5041 aagaatgccc gtgaccgtat ttcgaagaat cggcatgaag ctgtatattg taagcgcaac
     5101 caatgccggt acaaagccga tcgtccccag aataggaacc ataagggcta aaagagctat
     5161 cccgggaatg gtctgcaaaa tccccgctat tgaaagcaca ggccattgca gagattttac
     5221 gcgggtaacc agacatccca gcggtaggca gataaatatt cccagcgcca gggccagcat
     5281 ggtcaggagc agatgactgc ccagataatt ggggagcagg tcgagctggc tgacaatacc
     5341 ctcccagctc atttcttgtc gccccgtttc aggatttccg ccagtttctc tgattgctgt
     5401 cggggcgcgt gcagaagtct ctctgcatag gcatcgccag cttttgacat caattcgcct
     5461 ggtgtgccca tgactactat tttgccctcc tgcatgatcg ccacatggtc cgccagtatc
     5521 aaagcctcca tcatgtcatg agtaaccatg atgaccgtta gcttaagttc tgtctgcaag
     5581 ttcttgaatt catcctgaat cccggacctt gtaatcggat cgagcgcgcc gaagggctca
     5641 tccatcagca tcacatcggg acgtgccgcc agtgctcgtg ccacccccac acgctgtttt
     5701 tgtccacccg ataattccga gggcattttc tgagcatatt tatcgggatc aaggccggtc
     5761 agctccatta gttcgtcaac tcttgctttt atattggctc ttgaccagcc caatagcttc
     5821 gggacgatgc cgacattttc ggatatcgtc atatgcggaa acaggccgat ctcctgaaag
     5881 acatacccga ttctcctgcg gagttcgacc ggatttaaat caaggatatt tacgtcattg
     5941 actataattt cccctgagct tggttcgatc aggcgattta tcattttcag agtagtagtc
     6001 ttgccgcaac ccgattcgcc cgccagaatc aggatttcgc cgggattgac gttgaatgaa
     6061 atatcatcga cagcgtagct gatacgatcg taggattttg aaaggttttt gacagagatc
     6121 ataagcccgg ctcagaaagt ttgtctgcag aacagtataa gaagatatta cctctatcgc
     6181 cactaaaaat cgggggatgc cggctcaaaa atattaatcc ggcgactgcc ggactctacc
     6241 aactgagcaa agtgttctaa tgaattttct accttggtga gatttcaatt gtttttccct
     6301 tttgagcgct tttcctctgg tatcaaattt ttcgaagtgg atcagtttcc agggaatcta
     6361 ggctttggtg gattttactt ttccgagatt gtgtttttcg gtcctgattt tcaaattatt
     6421 agtctgtccg atataaacgc ggtcatattt ctcagaatag agaacgtaga cgtaatgatt
     6481 attgccagtg gcatccacga taatttctgg acacaaaaaa gctcctgctg caggactcga
     6541 acctgcgacc cggtggttaa cagccacctg ctctaccaac tgagctaagc aggagtactc
     6601 aattttatgt accttctatg ttcctgcgac cctgtgtggt taatccggcg actgccggac
     6661 tctaccaact gagctaagca ggagtatact aaatatatac gagtcttctc ctttatttcg
     6721 gccaattatt ataaataatt atgccctctt gtcaagtgct ttatttgcaa cagctatgcg
     6781 ttttgccaat gtccaaaaaa taaatacaat caagccgcaa attgttccca gaatggcccc
     6841 ggctaaaaca tcaaaggaat aatgtacacc cacaaaaatt cttgataaag ctataatcgc
     6901 cgctgatatg ataaagaacc acttatactt aggatagcgc caggcccata ttgccgcctg
     6961 accgaaagag ttggtggcat gccctgaggg aaaggactta ccgctggagc agtcgatcag
     7021 cacatgcagg ccctccagcg tcttgcaggg acgcatacgc ccgaccagcg gcttgatgat
     7081 tcctgatgag gcctggtc
//
LOCUS       DSEB01000304            1536 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1018120, whole genome shotgun sequence.
ACCESSION   DSEB01000304 DSEB01000000
VERSION     DSEB01000304.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1018120"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..>1536
                     /locus_tag="ENO07_05500"
     CDS             <1..>1536
                     /locus_tag="ENO07_05500"
                     /inference="COORDINATES: protein motif:HMM:PF01326.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDS01458.1"
                     /translation="HLGTISDFDPETFYSSSNFARIGRGSMGGKARGLAFANSIINDF
                     KLADFFEDIAITVPPSLVLATDIFDRFLEINELKDFAIKCRDDEELRSMFLAADFPPE
                     VASSLKVFLGLITDPLAVRSSSLLEDSRYLPFAGIYSTYMLPNNNVDDSVRLKNLLDA
                     IKLVYASTYAELAKGYIRATPYRLEEEKMGVLIQKLVGARYVDHFYPNFSGVVRSHNF
                     YPTAPMKASDGIAAVGLGLGRIVIEGEATVRFCPKYPRHVIQFSDVKDTLDYSQKEFY
                     ALDLNDSVNDPEDYEELKLVKYGLDTAEKDGTLTYVGSTYSVENNVITDGLSRSGPRV
                     ITFAPILKNRLFPLPEILRMIMEMGSWGMNSPVEIEFAVNLNTAGEKRREFAFLQIRP
                     LVKSHEADELNVDDVEEERLICQSPQVLGNGLIDDIYDVVVVDRDSFDRSKSSEVADE
                     VAKYNLELLSKDIPYILIGVGRWGSGDPWLGIPVTWDQISGARVIVETGFKGFKVVPS
                     QGSH"
BASE COUNT          371 a          337 c          425 g          403 t
ORIGIN      
        1 catctgggga caatctctga tttcgatccg gagacgtttt acagcagctc aaatttcgcc
       61 agaattggcc ggggctcaat gggagggaag gccaggggcc tggcatttgc gaattcaatt
      121 atcaatgatt tcaagctggc ggattttttc gaggacattg cgatcacagt gcccccctcg
      181 ctcgtattgg ccaccgatat ttttgaccgt tttctggaaa tcaatgagct gaaggatttt
      241 gccataaaat gccgggatga tgaggagctt agaagcatgt ttctggcggc ggatttcccg
      301 cctgaggttg ccagcagcct gaaagtattt ctgggattga ttaccgatcc tctggcggtg
      361 agatcatcat cgttgctgga ggactcgcgt tatctaccat ttgcgggcat ctattcgacc
      421 tacatgctgc caaataataa tgtcgatgat tcggtcaggt taaagaatct cctcgatgcg
      481 atcaaattgg tttatgcctc cacttatgct gagctggcca agggatatat ccgtgccacc
      541 ccctacaggc tggaagagga gaagatgggt gtcctgatcc agaagcttgt gggcgccagg
      601 tatgtcgatc acttctatcc gaatttctcg ggcgtggtca gatctcataa tttttatccg
      661 accgcaccca tgaaggccag tgatggtatc gcggctgtcg gtttggggct ggggaggatt
      721 gttatcgagg gtgaagcgac agttcgtttc tgtcctaaat atccgcgtca cgtaattcag
      781 ttttccgacg tcaaagacac gctcgactat tcacagaagg agttctatgc gctcgatctg
      841 aacgacagcg ttaatgatcc tgaggattat gaagaattaa agctggttaa atacgggctc
      901 gacaccgccg aaaaggacgg taccctgaca tatgtcggtt ccacatactc tgtcgaaaac
      961 aatgtgataa ccgatggtct ttcgcgcagc ggcccgcgag tgataacttt tgccccgatt
     1021 ttgaagaacc gtctatttcc gctgcccgag atattgcgga tgataatgga gatgggaagc
     1081 tggggcatga attcaccggt tgagattgaa tttgccgtaa atctcaatac tgcgggagag
     1141 aagcgcaggg aattcgcatt tcttcagatt cgtccactgg taaaaagcca tgaggccgat
     1201 gagctcaatg tcgatgatgt agaggaagaa aggctgattt gccagtctcc acaggttctt
     1261 ggaaacggcc ttatagatga tatctatgat gttgtggtgg ttgacaggga cagctttgac
     1321 cggtctaagt cgtcagaagt tgccgatgag gtggcaaaat acaatctgga gctgctctcg
     1381 aaagatatac cctatatttt aataggtgtg gggcgctggg gttcgggcga tccatggctc
     1441 ggcataccgg tgacatggga ccagatttcg ggcgccaggg tcatagtaga gaccggattc
     1501 aagggattta aagtggttcc ttcccagggc tcacac
//
LOCUS       DSEB01000305            5145 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001227, whole genome shotgun sequence.
ACCESSION   DSEB01000305 DSEB01000000
VERSION     DSEB01000305.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001227"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..877
                     /locus_tag="ENO07_05505"
     CDS             <1..877
                     /locus_tag="ENO07_05505"
                     /inference="COORDINATES: protein motif:HMM:PF02085.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01459.1"
                     /translation="AEWGLSLGVIAAAALVFMFISEQFPIFDESWRRRMEKSDVFTYA
                     FDKLSHVWNTVLMSGLQRVTLIAVLAIPLAWLLFYPPFKADDTTDGRIKPAIGLDQTR
                     TVLLIDGNRDGVKTEFPHVDHQNRLGKEQSCQKCHHIAMPRDNATPCYRCHSNMLDSA
                     EIFDHFGHMQLVAEKEKLDGLHPKNHSCSNCHNPALPNTASDAKACTECHKEDMKLGN
                     EPYARLQLASASPYRSAMHENCIECHKKEEIKQNKPNLAHCSTCHKSLEPVNLKIAKS
                     ADTIKALPEPLTLAR"
     gene            complement(894..1826)
                     /locus_tag="ENO07_05510"
     CDS             complement(894..1826)
                     /locus_tag="ENO07_05510"
                     /inference="COORDINATES: protein motif:HMM:PF04055.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDS01460.1"
                     /translation="MFVYGPVPSRRLGRSLGVSPIPPKTCSYSCVYCQLGRTTALESS
                     RKSYYPREKILDEILRKTPSSNADFITFVGDGEPTLCADLGWLIGKTKEQAGLPIAVI
                     TNGSLFFMEDVRRDLMPADVVLPTLDAGDQDTFKRINRPHKDIDYSTMLQGQIDFRRE
                     FRGRIWLEIMLVKGLNDTDEQLEKIKKAIDRIKPDRIYISVPIRPPAEQWVEIPLPES
                     ILKAQKILQASNPLDMLESGEFGLGEFSDAREAILEIGSRHPLRKSQAHEIETRLAGS
                     GVVEHMIEDGELFEIKYNNEIYLMPSHLKHYKNR"
     gene            complement(1828..2733)
                     /locus_tag="ENO07_05515"
     CDS             complement(1828..2733)
                     /locus_tag="ENO07_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDS01461.1"
                     /translation="MTELVIISGKGGTGKTSLVASIGALAENKVMVDCDVDAADLHLI
                     LNSNLLESHEFIGGKKAEIIEERCNSCAICLDYCRFDAISENEDKFKIDPLACEGCGI
                     CKHYCPEDAIAFEPVVCGHWYMSETIHGPLVHARLGIAQSNSGRLVSHLRKQARELAA
                     ENGHEMIIVDGPPGIGCPVIASITGSDYVAIVTEPSLSAFGDMKRVHELIQHFNVSCG
                     VIINKYDINPHMSAGIEAFAEENGIAVLGRIPFDKDMVAAQVAGKTIIDYQPGGLSDS
                     IREIWKNLQADLKKVEVKKIDLKAL"
     gene            complement(2726..3580)
                     /locus_tag="ENO07_05520"
     CDS             complement(2726..3580)
                     /locus_tag="ENO07_05520"
                     /inference="COORDINATES: protein motif:HMM:PF01656.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDS01462.1"
                     /translation="MIKISVASGKGGTGKTTIATNLAAALSARGKHVAYLDCDVEEPN
                     GGIFLKPTISAKSAVSVLVPQIDLEKCTFCGDCAAICEFNAIAVLKDNVIVFDSLCHS
                     CGGCYHVCPEDAIKEVPRQIGEIQIGAANGLKFMEGRLNIGESQAPPVTRQLKRGMPA
                     SDIVIIDAPPGTSCPVIEAISNTDYLILVSEPTPFGLNDLRLAVGMARELKLPFGVII
                     NRSDLGDNLTEDYCKNEGIEILLRIPFNRKIAEAYSDGRMACHQSDNYGENLYRLYEN
                     IAGRITND"
     gene            complement(3623..4021)
                     /locus_tag="ENO07_05525"
     CDS             complement(3623..4021)
                     /locus_tag="ENO07_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01463.1"
                     /translation="MPGGDRTGPLGLGPRTGRAAGYCSGYPAPGYANNRFGRGYGFGF
                     GFGRGRGFGRGLGMGYGRGWRHRNFAYGAYDIDYPYDPDYRDYPEAQARSADITKDEL
                     RYLRREARDLNKTLDDINARIKDLEEKKEA"
     gene            complement(4062..4424)
                     /locus_tag="ENO07_05530"
     CDS             complement(4062..4424)
                     /locus_tag="ENO07_05530"
                     /inference="COORDINATES: protein motif:HMM:PF02579.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dinitrogenase iron-molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HDS01464.1"
                     /translation="MILAISSQGPTLESMVDPRFARAPYFIIYDTEKEEFEAINNSEN
                     ANAMHGAGVQASQELMSHKPDIAISGNFGPRAFQVLNSSGVRTALWAEGKVSEAIELA
                     KSNQLEFTQNANVRGHWQ"
     gene            4843..>5145
                     /locus_tag="ENO07_05535"
     CDS             4843..>5145
                     /locus_tag="ENO07_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01465.1"
                     /translation="MKKLFAFTISLAISFCFLILASPAEAGTRFGGGIHYLRTLGDIK
                     DAPEFDENAVGFMGSVLFTHTMFRLEGAIEVIPDFGASEKTMFEPQAYAMIGQFIYG"
BASE COUNT         1259 a         1308 c         1207 g         1371 t
ORIGIN      
        1 ggcggagtgg ggcttgagcc tgggtgttat cgccgcggca gcgctggtgt ttatgtttat
       61 atcggagcaa tttccgatct tcgatgaaag ctggagacgc cgcatggaaa agtcggatgt
      121 ttttacctac gctttcgata aattatcaca cgtctggaat accgtcctga tgagcggcct
      181 gcaaagagtg accctgatcg ctgtgctggc gattccgttg gcctggcttt tgttctatcc
      241 gccttttaaa gcagacgaca ctactgacgg cagaatcaag ccggctatcg gtctcgatca
      301 aacgcgcacg gttctgctga ttgacggaaa ccgggatggt gtcaaaactg aatttcccca
      361 tgtggatcat caaaatcggc tgggtaaaga gcaatcgtgc cagaaatgcc atcatatcgc
      421 catgcccaga gataatgcca ctccctgcta ccgctgtcat tcaaacatgc ttgattcggc
      481 tgagatattt gaccatttcg gccatatgca gctggtggcg gagaaggaaa aacttgacgg
      541 cctgcatcca aaaaatcatt cctgctccaa ttgtcacaat cctgctctgc ccaatactgc
      601 ctccgacgca aaagcctgta cggagtgcca caaggaggac atgaaattag gtaatgaacc
      661 gtatgccaga ctccagctgg caagcgccag cccctatcgc agcgccatgc acgagaactg
      721 tatagagtgt cataagaagg aagaaataaa gcaaaataag ccaaacctcg cgcattgttc
      781 aacctgtcat aaatcactgg agccggtaaa tctgaagatt gcaaaatcgg ccgatacaat
      841 taaagcactg ccggagccat taacgcttgc tcgttgaggg taacaaaact atatcatcga
      901 ttcttgtaat gcttaaggtg tgagggcatc aaatagattt cgttattata ttttatttcg
      961 aacagctcgc catcctcgat catgtgttca acgacgcccg atcccgccag ccttgtttcg
     1021 atttcgtgcg cctgagattt tcttaaagga tgccgtgagc ctatttccag aatagcctcc
     1081 cgggcatcag aaaactctcc gagtccgaac tctcccgatt caagcatatc cagaggattc
     1141 gatgcctgaa gaattttctg tgctttaagg atagattcgg gtaagggtat ttccacccat
     1201 tgttctgcag gcggacggat gggcacgctt atgtaaatgc ggtccggttt tatacgatca
     1261 atagccttct tgatcttttc caattgctca tcggtgtcat ttaaaccctt caccaacatt
     1321 atttcgagcc atatccggcc cctaaattct ctccggaaat caatctgtcc ctggagcatg
     1381 gtcgaataat ctatatcttt atgtggtctg tttattctct tgaacgtgtc ctgatcgccg
     1441 gcatcgagtg ttggcagaac aacatctgcg ggcatcaggt cccggcgcac atcctccata
     1501 aaaaacagcg agccgtttgt aatcactgca atcggcagtc ccgcctgctc ctttgttttg
     1561 ccgataagcc atcccagatc agcgcacaga gtcggttcgc catcgccgac aaatgtaatg
     1621 aaatccgcgt ttgaggaagg cgttttacgc aggatttcat ccagaatctt ttccctcggg
     1681 tagtaacttt ttctggatga ttccagggcg gtggttcttc caagctggca atacacgcat
     1741 gagtaggaac aggttttggg tggaatggga cttactccca atgagcgtcc cagtcttcgc
     1801 gatgggaccg ggccgtagac aaacatatta tagtgctttc aaatcgatct ttttgacttc
     1861 cacttttttt aaatcggcct gcagattctt ccatatctct ctgatgctat cagaaaggcc
     1921 gccgggctgg tagtcaataa tggttttgcc cgctacctgg gccgctacca tatctttgtc
     1981 aaaaggtatc cggcccagaa cagcaatgcc gttttcctct gcaaaggctt cgattccggc
     2041 ggacatatgg ggattgatat cgtacttatt gataatgact ccgcaggaga cgttgaaatg
     2101 ctgtataagc tcatgcactc ttttcatgtc cccaaaggcg gaaagtgatg gttcggttac
     2161 aatggcaaca taatctgaac cggtgattga tgcgatcact gggcatccga tcccgggagg
     2221 accatcaact ataatcatct catggccatt ctcggccgcc agttcacggg cttgcttgcg
     2281 cagatgtgaa accagccgtc cggaatttga ttgggcaatt cccagacggg catgtacaag
     2341 cgggccatga atagtttccg acatgtacca gtgaccgcat accaccggtt caaacgcaat
     2401 cgcatcctca gggcagtagt gcttgcaaat tccgcatccc tcgcaggcta agggatctat
     2461 tttaaattta tcctcatttt cggagatggc atcgaaacgg cagtagtcca ggcagatagc
     2521 acaggaattg cagcgctctt cgataatctc ggcttttttc ccgccaatga attcatggct
     2581 ctcgagcaaa ttgctattca gtatcagatg aagatcggca gcgtcaacat cacagtcgac
     2641 cattaccttg ttctcggcca gcgctccaat tgaagctacc aggctggtct tcccggtacc
     2701 gcccttaccg ctgatgatta ctaactcagt catttgttat cctgcctgca atattttcgt
     2761 aaagcctgta tagattttcg ccataattat cgctctggtg gcacgccatc ctgccgtcag
     2821 aataggcttc ggcaattttg cggttgaacg ggattcgcaa aagaatctct attccttcat
     2881 ttttacagta atcctcggtg aggttatcgc cgagatcgga acggtttatg atcaccccaa
     2941 agggcagctt cagctcacgg gccatgccca ccgccagcct gagatcgttt aagccgaatg
     3001 gcgttggttc cgagaccagg atgagataat cggtattgct gatcgcctcg atcacggggc
     3061 atgaggtccc gggcggggca tcgataatga ctatatccga ggcgggcatc ccccgcttca
     3121 attgcctggt aacaggaggg gcctgggatt cgccgatatt aagccggccc tccataaact
     3181 taaggccgtt ggcggctccg atttgaattt ccccaatttg cctgggaacc tcttttatcg
     3241 cgtcttccgg gcagacatga tagcatcccc cgcaactgtg acagagagag tcgaagacta
     3301 taacgttatc ctttaaaacg gcaatggcgt tgaactcaca aatcgcagcg cagtcgccgc
     3361 agaaggtgca tttctccaga tcgatctgcg gcaccagcac gctgaccgcg gatttagcgc
     3421 tgatggtagg cttcaggaaa attccgccat taggctcctc gacatcgcag tcgaggtaag
     3481 ccacatgttt gcccctcgca ctcagcgccg cagcaagatt tgtggcgatg gttgtcttac
     3541 ctgtaccgcc ttttccactg gcgacagata tcttgatcaa gtcaaatcct ttccggccgg
     3601 ccgaaaattt tgcccgatct gatcaggctt ctttcttttc ttcaaggtct tttatgcgtg
     3661 cattaatgtc atccagagtc ttatttaaat ctctggcttc gcgccttaaa tatcgcaatt
     3721 catcttttgt aatatcagcg ctcctcgcct gtgcctcggg gtaatcgcgg taatccggat
     3781 cataaggata atctatatca taggctccat aggcgaaatt gcggtgacgc catccacgtc
     3841 cataacccat gcccaagcct cgtccgaatc cccgacctcg cccgaatcca aagccgaaac
     3901 cgtatcctct tccaaaacga ttgttggcat aaccgggtgc cgggtaaccg ctgcagtaac
     3961 ctgccgcacg gcctgtcctt ggtcctaatc cgagaggacc tgtcctgtct ccacctggca
     4021 tcttatactc cttgcttaaa aaattaaagt aatacctata tttattgcca atgtcccctg
     4081 acattggcat tctgcgtaaa ttccagttga ttcgatttgg caagttctat cgcctcggaa
     4141 accttgccct cggcccagag cgccgtcctg actccggagc tgttcaagac ctgaaatgcc
     4201 ctgggtccga aattcccgga tattgcaatg tccggcttgt gggacatcaa ctcctgcgaa
     4261 gcctgaacac cagcgccatg catcgcgttg gcattttcag agttgttgat tgcctcaaat
     4321 tcttccttct cagtatcata aattatgaag tagggtgctc gcgcgaatct cggatcaacc
     4381 atactttcga gagtggggcc ctgtgatgaa atcgccaaaa tcatatgtcc tccaaatggc
     4441 tttcaggttt cgagacttat ttcacatctc ttctattgat atgcataagg catgcctatt
     4501 agcatatgag aataccgata tatgtaaggc gttgatttga agcaaattgc aagtaatcaa
     4561 ccttgactga tgttgctgga gggcaactcc ataattgcaa tattgcaaca tagctggttt
     4621 tagagggttg cattaatgca aaaggccgta atatgtaatt aagtttccac catggctcct
     4681 gtcggtagaa tcaagaatga ccaaactccc tccgagggga acaggattcc ctgcaaatct
     4741 gttctagact aaaatcttac aaaaggacgg taggtcttaa tggtagcaac atccatatct
     4801 tcagcacaca tattgtaaaa tagatcatga aaggagtgga tcgtgaaaaa attattcgca
     4861 tttacgatct cgctggcgat cagcttctgt ttcttgatac ttgcgtcgcc cgccgaggcc
     4921 ggtacgcgtt tcgggggagg cattcactac ctcaggaccc tgggtgatat taaggatgct
     4981 ccggaattcg atgagaatgc tgtcggtttc atgggctcgg tcttgttcac gcatacgatg
     5041 tttaggctgg aaggagctat tgaagtgatt cccgattttg gtgcgagtga aaagactatg
     5101 tttgaaccgc aggcgtacgc tatgatcggg cagtttatct atgga
//
LOCUS       DSEB01000306            4612 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001582, whole genome shotgun sequence.
ACCESSION   DSEB01000306 DSEB01000000
VERSION     DSEB01000306.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001582"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..209)
                     /locus_tag="ENO07_05540"
     CDS             complement(<1..209)
                     /locus_tag="ENO07_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDS01466.1"
                     /translation="MDNKVRIIAGDLKNRPIKIPRDTETRPALVLVRRMVCDTLAPDI
                     PDARVLDLFAGTGAFVFEMISRGAK"
     gene            complement(266..517)
                     /locus_tag="ENO07_05545"
     CDS             complement(266..517)
                     /locus_tag="ENO07_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01467.1"
                     /translation="MSAEPVKKLRGEHCLNIFISYELKKRINALAHKYDRTMADIVRM
                     LMRVGIPIMEGLSKAEEEMMKDYIQLFRKMRRVKELKDM"
     gene            complement(514..1011)
                     /locus_tag="ENO07_05550"
     CDS             complement(514..1011)
                     /locus_tag="ENO07_05550"
                     /inference="COORDINATES: protein motif:HMM:PF08281.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01468.1"
                     /translation="MPRRIVRHNWIYQMLSPELWGEDEISGRQMRIGQAVRLELEKLP
                     DSEREFVELYWFQGRSTSEIARLLNCKPYNLVNLNRRIMRKLKNSLADFVEEEFRLRA
                     DHQGKCRICCHPDVEKINLKLKSKKPHETYRGLIRLLKTDYGIDIKTPQAIIGHLKYH
                     LREEL"
     gene            complement(1136..1948)
                     /locus_tag="ENO07_05555"
     CDS             complement(1136..1948)
                     /locus_tag="ENO07_05555"
                     /inference="COORDINATES: protein motif:HMM:PF04055.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDS01469.1"
                     /translation="MDNSRKIEGFENRRVLPLQKGNIYGPVKSRRLGSSLGLNLLPRS
                     YKLCSFNCIYCQYGLNPPQSPDMVSALYDELPTVSAIKKAILAALASLREKIDYITFS
                     GNGEPTLHPDFPAIVEVVRDIRDRYASHVKLAILSNSTTINKPEMVEALMQLDTPIMK
                     LDAGSEKLYKRINRPAGGVDFNKIVEGLIEFNHPNLIIQALIMGGPDLNASDDNIIKW
                     ASLLEKIKPVEVQIYSLDRPPARPDIIKLSRKELEEIAGRASELSGVPVRAF"
     gene            2062..2775
                     /locus_tag="ENO07_05560"
     CDS             2062..2775
                     /locus_tag="ENO07_05560"
                     /inference="COORDINATES: protein motif:HMM:PF02517.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDS01470.1"
                     /translation="MTAAIDQDFKINFLSVILIIFLCLIWPLSISLLFEPPADFAPAE
                     IENPVFVFYLPTIGFQLIVFFFVFLVSRGEGSGIVALGYRNFKLTHVIYAIVFLFASL
                     FLLKIVKLFLDKLDFMKFHDPSPLLPQTLEGRLVWILMCLVVAVNEETAYRGYLITRL
                     NKILRSMPLAIIIATAGFAAGHLYQGVGGTVLLFVYGLMFVFLYLATKSLWPCVIAHF
                     LHNALAIFVQMPIILPPFC"
     gene            complement(2812..>4612)
                     /locus_tag="ENO07_05565"
     CDS             complement(2812..>4612)
                     /locus_tag="ENO07_05565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HDS01471.1"
                     /translation="NIYVQHFDGNANKVEDNFLVSEGLGNEAFDLFFAQNPGDSLLAV
                     WANIGVRSEIVAQVFDQTGKVSGGNFTVSDGLVLGERFSPNAIFRVDGSVFISFTDTR
                     SAFIDIYAQILNSDFSLSGNNFKLTTGATGAQQVGSDIVRMAGDDFGVVWTDLRNDDG
                     DIFFQRGDGFGNPYGSNLKLNDDLGRSLQDDPSIGSATNGLALAAWVDGRSSGELTGI
                     NIFAQRIGANGQKVGSNFPVNDDPAGTAVLQAEPECDIAPSGKAVVAWRDRRNGVDDI
                     FAQLYDASGSPVGMNFKVNLETLPCKNPKIAMANDESFMIAWLIELEAKSYIKFQLYS
                     AQGTPAGGNMLIPADTSINQQLDFDLSVNPYFGFYVLAWINQNNAEREINAMLLNPDG
                     SSDSTVMVISDAVNLGFEDISVDMDTDNSYVVAWSDMRSGVRRSYLSFVDAGLTVYPD
                     QLISQKAGVAREQEPAIAINGRTWFASWSDNRNVGDGYDIYANSNLYNPTSAGEDGDS
                     PVPDLLALSQNYPNPFNPITRIDFNLPRDMTEVSFEVYNVLGRKVYSETMKNMSAGSY
                     TIEFNGEGLSSGIYLYRLKADDRIMTRKMSLLK"
BASE COUNT         1137 a         1200 c         1013 g         1262 t
ORIGIN      
        1 cttttcgcac cgcgcgagat catctcaaat acaaatgctc cggtgccggc aaaaagatcg
       61 agcaccctgg catccggaat atcaggcgcg agagtgtcac agaccatccg cctcaccaat
      121 accagggccg gacgggtctc ggtatccctg ggaattttga tcggccggtt ctttagatcg
      181 ccggcaatga tcctgacctt attatccatg ccgtgatgtt ataacattaa tacggtttcg
      241 tcaatctgat aatgtcatgt cattatcaca tatcctttag ttctttcacc ctgcgcatct
      301 tacgaaaaag ctggatataa tccttcatca tctcctcctc ggccttggac agcccctcca
      361 taatcggtat ccccactctc atcaacatcc gtacgatatc agccatggtc cggtcgtact
      421 tgtgcgccag ggcattgatg cgctttttca gctcatacga gataaagata tttaagcagt
      481 gttcgcccct cagttttttg acaggttctg cgctcataat tcctccctta gatgatattt
      541 aagatgcccg ataatagcct gcggtgtttt tatatcgatt ccatagtcgg tcttcagaag
      601 cctgatcagg ccccgatagg tttcatgcgg cttttttgat ttcagtttca ggttgatttt
      661 ctcaacgtcc ggatggcagc aaatccgaca tttgccctga tggtcagccc tgagtctgaa
      721 ttcctcctcc acaaaatcgg ccagactgtt tttcagcttg cgcatgatac gcctgttcag
      781 gtttaccaga ttgtagggtt tgcagttcag aagcctggct atctccgatg ttgatcggcc
      841 ctgaaaccag tacagctcaa caaactcccg ctcagaatca ggcagctttt ccaactcaag
      901 cctgaccgcc tgaccgatac gcatctggcg cccgctgatt tcatcctcgc cccagagctc
      961 cggactcagc atctggtaaa tccaattatg tcgtactatt ctccgcggca tacggcctcc
     1021 tttcggagac caatattaaa tcatccagga attatgtaaa acgcatcata attacatgaa
     1081 atcagaggaa ttgagctgag aatttcctgg gaatcacatg aaaaatgatg ggcccttaaa
     1141 aagctcttac cggaacgccg ctcaattctg atgcacgacc ggcgatttct tccagttcct
     1201 ttcgggaaag cttaataata tccggcctgg cagggggacg atcgagggaa taaatctgga
     1261 cttccacagg cttgattttc tcgagcaggc tggcccattt tatgatgttg tcgtccgagg
     1321 cattcagatc gggcccgccc atgatgaggg cctgaataat caaattagga tgattgaatt
     1381 ctatcaaacc ttcaactatc ttattgaaat caactccccc ggccggacga tttattctct
     1441 tgtacaattt ctccgaaccg gcatcgagct tcatgatagg tgtgtccagc tgcattaatg
     1501 cttcgaccat ttcgggcttg ttgatcgtgg tggaatttga caagatcgca agtttaacat
     1561 gggatgcata tctgtcgcgt atatccctga caacttccac aattgctgga aaatcgggat
     1621 ggagagtcgg ctccccattg cccgagaatg taatatagtc gatcttttcg cgcaatgatg
     1681 ccagcgccgc caaaattgct ttttttatgg cagagactgt gggaagttca tcgtaaagcg
     1741 cggaaaccat atccgggctc tgaggaggat tcaatccata ctggcaataa atgcaattga
     1801 atgaacacag cttatagctg cgcgggagca ggttcagacc gagagatgat cccagccgac
     1861 gcgatttgac cggcccataa atattgccct tctggagcgg aagtactctg cgattttcaa
     1921 agccctcaat tttacgggaa ttgtccataa ttgcctatat tatccatcat tttataaaaa
     1981 gcaagttttt atttgccctg atcgcgtaaa cccttacctt ttatcagagc gcgacttcga
     2041 aagaaaaggc aggttatcag aatgaccgca gcaattgatc aggatttcaa aatcaacttc
     2101 ctttcagtga tcctgattat attcctctgc ctgatctggc ccttatcgat ttcactattg
     2161 ttcgagcccc cggcagattt tgcgcccgca gagatcgaaa atccggtctt cgttttctat
     2221 ctgccaacta tcggatttca gcttatcgtt ttctttttcg tattcctcgt gagccgtgga
     2281 gaaggcagtg gaattgtcgc gcttggctat aggaatttca agcttactca tgtaatatac
     2341 gcaattgtat ttcttttcgc atcacttttc ttattgaaaa tagttaaatt atttctcgat
     2401 aaacttgatt tcatgaaatt ccatgacccc tcccccctct tgccccagac tctggagggt
     2461 aggctggtct ggattttgat gtgcctggtg gtcgcggtaa atgaggaaac cgcctatcgg
     2521 ggatacctga taacccggct taataaaata ctcaggtcaa tgcctctggc aattattatc
     2581 gcaaccgcgg gctttgccgc gggccatctc taccagggcg tcgggggtac ggtgctgctg
     2641 tttgtttacg gcctgatgtt tgtcttcttg tacctggcta caaagtcgtt atggccctgc
     2701 gtgattgcgc attttctcca taacgccctc gctatcttcg tccagatgcc gataatattg
     2761 ccgccatttt gttaacgccc ccgggatttc ggcatattgt gcgagggtga tttatttcaa
     2821 caaagacatc ttcctcgtca tgattctgtc atccgctttc agcctgtaga ggtagattcc
     2881 ggatgacaac ccttctccat tgaattcaat cgtgtatgat ccggcggaca tatttttcat
     2941 tgtctccgaa tatactttcc ggcccagaac attataaacc tcaaaactca cttcggtcat
     3001 gtcacgaggc agattgaaat caattctggt tatcggattg aatggattag gataattctg
     3061 tgagagtgcc aataaatcgg gaacagggga atcgccgtcc tccccggcag aagtcggatt
     3121 atacagattg gaattcgcat atatatcata accgtctccg acattacggt tatcgctcca
     3181 cgaggcaaac caggtacgtc cattaatcgc tattgccggc tcctgctcac gcgcgacacc
     3241 tgctttttgc gaaatcagct gatcgggata taccgtcaaa ccggcatcga cgaaactgag
     3301 gtatgatctc cttactcccg aacgcatatc gctccaggcc acaacatagc tgttgtcagt
     3361 atccatatcg accgagatat cttcaaatcc gaggttgacc gcgtcggata tgaccatcac
     3421 cgtggaatca gacgatccgt caggattgag cagcattgcg ttgatctccc tttcagcatt
     3481 attttgattg atccatgcca gcacgtagaa tccaaaatag ggattcaccg agagatcaaa
     3541 atcgagctgc tgatttattg atgtgtccgc gggaatcagc atgttcccgc ctgccggggt
     3601 cccctgcgca ctatataatt gaaacttgat ataagatttg gcttccaact caatcagcca
     3661 ggcgatcata aagctctcgt cgttggccat ggcaatcttt ggattcttgc atggcaaagt
     3721 ttctaggttc accttgaagt tcataccgac ggggctcccg gaagcatcat aaagttgagc
     3781 gaaaatatca tcgacaccgt tgcgcctgtc tctccaggcg accaccgcct ttcccgaggg
     3841 agcaatgtcg cactcaggct cggcctgcaa tacggcagtt ccggcaggat cgtcattaac
     3901 cggaaagtta ctgcctactt tctgcccatt ggcaccgatc cgctgagcga agatattgat
     3961 tcctgtcagc tcgccggaag atctcccgtc aacccaggcg gctaaagcca acccgttagt
     4021 ggcggatccg atcgagggat cgtcctggag cgatctaccg agatcgtcat taagtttcaa
     4081 attcgagccg tatggattgc cgaacccatc cccgcgctga aagaaaatat cgccatcatc
     4141 atttctcaga tctgtccaga ccacgccaaa atcatcaccc gccatccgca caatatccga
     4201 tccaacctgc tgagcgccgg tcgcacctgt ggtcagcttg aaattgtttc cggataagga
     4261 aaagtcgctg tttaaaatct gtgcgtaaat atctatgaat gcggaacggg tatccgtgaa
     4321 gctgataaaa accgatccat caacacggaa tatggcgttc gggctgaatc tttcacccag
     4381 taccaggccg tcagacacag tgaaattacc gccgctgact tttccggtct ggtcaaaaac
     4441 ctgggccacg atttcggacc tcacaccaat attggcccag accgccagca ggctgtcccc
     4501 gggattctgg gcgaaaaata aatcaaaagc ttcattgccg agcccctcgc tgaccagaaa
     4561 gttatcctct actttatttg catttccatc aaaatgctga acatagatat tc
//
LOCUS       DSEB01000307            3029 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004037, whole genome shotgun sequence.
ACCESSION   DSEB01000307 DSEB01000000
VERSION     DSEB01000307.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004037"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..394
                     /locus_tag="ENO07_05570"
     CDS             <1..394
                     /locus_tag="ENO07_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="triosephosphate isomerase"
                     /protein_id="HDS01472.1"
                     /translation="KPIVCVGEQLEEREADKTEEIIRTQVEGFSAEVDYSRVEEIVIA
                     YEPVWAIGTGKTATPDQAVEVHKLIRKIWKEKYDKQIADGLQILYGGSVKPENSDDLL
                     GEEEIDGALIGGASLSKDKFLKIISSAT"
     gene            364..822
                     /gene="secG"
                     /locus_tag="ENO07_05575"
     CDS             364..822
                     /gene="secG"
                     /locus_tag="ENO07_05575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDS01473.1"
                     /translation="MLENHIISDLRRKLLFSILIVLHIFISLGLIISVLMQSAKGEGL
                     AGAFGGGGGVSGAVFGGRGAATFLSKATTVLAVAFMINCILLTFSGGGTGVPSSRITG
                     EAQKAMEDAQVSPMQLPAEGQPQQQVQPGQQQGQQEIQVEPIMPPQTEGE"
     gene            839..920
                     /locus_tag="ENO07_05580"
     tRNA            839..920
                     /locus_tag="ENO07_05580"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:873..875,aa:Leu,seq:gag)
     gene            1280..1519
                     /locus_tag="ENO07_05585"
     CDS             1280..1519
                     /locus_tag="ENO07_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01474.1"
                     /translation="MKPLAGILIVISGIGLIGGIILQLTAASKAESDEFHFKPWRWKV
                     KKEWFRAPGYSLYIASGIMFFTGCITGIIYWLFFA"
     gene            1538..2170
                     /gene="nth"
                     /locus_tag="ENO07_05590"
     CDS             1538..2170
                     /gene="nth"
                     /locus_tag="ENO07_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDS01475.1"
                     /translation="MDKKDRARKAVSLLKEEYPDSRCTLDFKNPHQLVVATILSAQST
                     DERVNMITPALFKKYKSPRDFAEADLEELQENIKTAGLYRNKAKSIKNCMKTVVEEYN
                     GKIPKTMEELVKLPGVGRKTANVILGAAYNIASGIAVDTHVTRLANLLNLTNRKEAPK
                     IEKDLMELIPRKDWIIVSHLLIDHGRKVCIARNPRCHECVLNKICPSSKV"
     gene            complement(2172..2789)
                     /gene="amrA"
                     /locus_tag="ENO07_05595"
     CDS             complement(2172..2789)
                     /gene="amrA"
                     /locus_tag="ENO07_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HDS01476.1"
                     /translation="MSYLSAVVYKSGDGSDDGNAALTGEDYLSEKQQQKLLEIARKSI
                     EAYIANQSVPEFDINDSILEAPGAAFVTINKNHQLRGCIGYTEPILPLYQTVSTCAIK
                     AASEDPRFEPLSKNEYADIELEISVLTPLKKIEDVNKIEVGKHGLMMQRGYNRGLLLP
                     QVAVDFGWDRMQFLDATCRKAGMPPGCWKENCDIYIFSAEVFEEK"
BASE COUNT          805 a          706 c          758 g          760 t
ORIGIN      
        1 gaaaccgatc gtatgtgttg gggaacagct tgaagaaaga gaggccgata aaaccgagga
       61 gataatcagg actcaggtgg aaggtttttc tgccgaagtt gattatagcc gggtcgagga
      121 gattgtgatc gcatatgagc cggtctgggc gatcggcacc ggcaaaaccg ccaccccgga
      181 tcaggccgtt gaggtacata agcttatacg caaaatctgg aaagagaaat acgataaaca
      241 aattgccgat gggcttcaga ttctgtatgg cggctcggta aaaccggaaa attcggacga
      301 tttacttgga gaagaagaga ttgacggcgc tcttattggt ggagcttcgc tttcaaaaga
      361 taaattcttg aaaatcatat catcagcgac ctgaggagga agcttttgtt tagtattctt
      421 atagttctgc atatattcat cagcctgggg ctgataatct cagtcttgat gcagtctgcc
      481 aagggcgagg gcctagcggg cgcgtttggc ggcggcgggg gagtctccgg agccgtattc
      541 ggaggacgcg gcgcggccac atttctgtcc aaagcgacta ctgttctggc ggtggccttc
      601 atgattaact gcatcttgct gactttctcc ggtggaggca ccggcgtacc ctccagccgt
      661 atcaccggcg aggctcagaa ggcaatggag gatgcccagg tttcaccgat gcagctcccg
      721 gcagaagggc agccgcaaca gcaggtgcag cccggccagc aacagggtca gcaggaaatt
      781 caagttgagc ctatcatgcc gccccagacc gagggtgaat aacagataga ttataaatgc
      841 cgaagtggtg gaattggtag acacgccatc ttgagggggt ggtaccgcga ggtgtgcggg
      901 ttcaaatccc gccttcggca tttagtttct cagccccatt tcaggggcat ttttatgtct
      961 gataagtcct cagaagagcc cgaaatatgg ttgcaaatat gcaacgcctg cggaaaaaat
     1021 ctgtaatatt ctaatcaaat aatcccttgc gagccaagaa agtgcgttta aaatcaggcc
     1081 cgcagccggc ctcaggaggc cctctgaagc cttatttttc ttgacttttt gggaggtcgt
     1141 gatattatta aaaacgacag tatatctgtc tacccccctc cttaggctgg ttgcattctg
     1201 taaccagccc tttttttgtt gactgcactt tgtgagtcat ttatttgtaa tttatggata
     1261 ttcataggag gacatcgcaa tgaaaccgct ggcaggaatt cttattgtta ttagtggaat
     1321 tggattaata ggcggaatta ttttgcagct gacagctgcg agcaaggctg aatcagatga
     1381 attccatttc aagccctgga ggtggaaagt aaaaaaggaa tggttcaggg cgccgggata
     1441 ttcgctttat atcgcctcag gtataatgtt cttcaccgga tgcataaccg gcataattta
     1501 ctggttattt tttgcttaaa atcgattata attccgtatg gacaaaaaag accgcgccag
     1561 gaaagcagtc tcgcttctga aagaagaata tcccgattcc agatgtacgc ttgacttcaa
     1621 gaatccgcat cagcttgtgg tcgccacgat actttccgct caaagcactg atgagcgtgt
     1681 gaatatgata acgcccgcgc ttttcaagaa atacaaatcc cccagagatt ttgccgaagc
     1741 tgacctggag gaattgcagg aaaatatcaa gacagcggga ttatatcgca acaaggccaa
     1801 atcgatcaaa aattgcatga agacggtggt ggaggaatac aacgggaaaa tcccgaaaac
     1861 aatggaggag ttagtcaagc tgccgggtgt gggacgaaag acggccaatg ttattcttgg
     1921 tgcagcttat aatatagcat ctggaatagc ggtcgacacc catgtcaccc gtctcgccaa
     1981 tctcctcaac ctgacaaaca ggaaggaggc gcccaaaatc gagaaagatt tgatggaact
     2041 gattccgagg aaggattgga taattgtctc acatctattg atcgatcatg gacggaaagt
     2101 ctgtattgca agaaatccaa gatgccatga atgcgtatta aataagatct gcccctcaag
     2161 caaagtgtag cttatttctc ttcaaagacc tcggccgaga agatataaat atcgcagttc
     2221 tctttccagc atccgggagg catgcccgct ttgcggcagg tggcgtcaag aaattgcatc
     2281 ctatcccatc caaaatcaac cgcaacttga ggcagaagca acccgcgatt atagcctctt
     2341 tgcatcatca agccatgctt gccgacttcg atcttattga catcctcgat tttcttgaga
     2401 ggtgtcagca ctgaaatctc taattctata tcagcatact catttttgct caagggctcg
     2461 aaacgcggat cctcggaggc cgccttaatc gcgcatgtgg aaaccgtctg gtaaagcgga
     2521 agaatcggtt cggtataacc gatacatccc cgcagctgat gattcttgtt tatggtaaca
     2581 aacgctgctc ccggcgcctc cagaatgctg tcgtttatgt caaattccgg aaccgactgg
     2641 ttcgcaatat aggcttcaat agatttgcgg gcaatttcca ggagtttctg ctgctgcttt
     2701 tcagaaagat aatcctcacc cgtgagcgcc gcgtttccat catctgagcc atcgccggat
     2761 ttatatacga ccgcagataa atagctcacc accgattgtg tatttcctgc atattcgccc
     2821 gaatcggaat gcttgaggat tttagcctta tcagccccca gttttttagc aatttcaagc
     2881 gctaccgcca ccggcgccgc cccgcaaagc tgggctctgc cattttcaga ggcacgccat
     2941 aaaccttcac cgtctatcga ggcgatgtac tcggccgaga ctgaatctat ggaagcgcat
     3001 tgcctgcggc tcttgtaatg ggtcatatc
//
LOCUS       DSEB01000308            3031 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004031, whole genome shotgun sequence.
ACCESSION   DSEB01000308 DSEB01000000
VERSION     DSEB01000308.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004031"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..367
                     /locus_tag="ENO07_05600"
     CDS             <1..367
                     /locus_tag="ENO07_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HDS01477.1"
                     /translation="VIGTIKGDLHDIGKNLVGIMLKGAGFEVIDLGNDVTPDRFVQAA
                     KDEKADAVGMSALLTTTMTGMKDVIELMKSEGIYGKIKVIIGGAPVNREFAEQIGADS
                     YAYDGMKAVEILKEYVKDK"
     gene            369..1259
                     /locus_tag="ENO07_05605"
     CDS             369..1259
                     /locus_tag="ENO07_05605"
                     /inference="COORDINATES: protein motif:HMM:PF02574.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydrofolate--homocysteine
                     methyltransferase"
                     /protein_id="HDS01478.1"
                     /translation="MRDFLYRIRAGEIIVADGAMGTMCFEAGLRVGDCPELLNIENPQ
                     LIEDITRSYLEAGSQIVQTNTFGGTALKLAPYGLQKRSAEINEAAVKIARRAAGDRAY
                     ISGSCGPSGKILQPYGDTEPEIIYASFREQINALIEAGADIITVETMVDLEEAKLAIK
                     AARQISSTIPVMAAMTFDSTPNGFFTIMGISIEDAAVGLKEAGADIIGSNCGNGIRDM
                     VMIASGFRQATDMPLIIQSNAGLPKVENGKTVYTETPEMMAEHAKDLMVLPVSVIGGC
                     CGTTPEHIRALRKMVDSWAA"
     gene            1247..1756
                     /locus_tag="ENO07_05610"
     CDS             1247..1756
                     /locus_tag="ENO07_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01479.1"
                     /translation="MGSLIGRFLTLAGAAIVVISLFTPVIQMEFEVPEQRLGQIVLKP
                     RPVMVESSLYELGDKLSDLDGSGAELPEVLDYLWLIFLGLAVINGLLAIKPSFPRILR
                     ALVGILPLALLVIVIWQTVSNPNVAIGYADLLNYFVKGFYVLLAGTLIIFLGTLMTKG
                     KRIHSSRPS"
     gene            1907..2998
                     /gene="nadA"
                     /locus_tag="ENO07_05615"
     CDS             1907..2998
                     /gene="nadA"
                     /locus_tag="ENO07_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDS01480.1"
                     /translation="MALTFNPIPASYRRLSDDELTERIRRKKQASGDKLVILTHHYQR
                     KEVAALGDYIGDSYALSKIASELEAEIIVFCGVHFMAEAADILSTDKQTVYLPNPLAG
                     CPMADMAAITNVMHAWDQLKELLPDTKIIPIAYMNTAADLKAFCGRNDGLICTSSNAT
                     GAFRWGLERGDKVFFYPDEHLGRNTANKLAIPENEIAVWDYRLRRLGGNTPEKIKNAK
                     VIIWKGYCHVHTTFTPEHVQRMREKFPDVKVVVHPECPQEVVKLADADGSTAFIVKYV
                     EEAEPGATIAIGTEINLVDRLAHENPDKKIMELSGNTCAMCVNMYRTTLNDLAWCLEN
                     LDDIRPIEVRPEIKEEARLSLSRMLEVPR"
BASE COUNT          818 a          667 c          799 g          747 t
ORIGIN      
        1 agtaatagga acgataaaag gcgatctgca tgatattgga aaaaacctgg tggggattat
       61 gctcaaggga gcaggctttg aggtgataga cctgggaaac gacgtcactc ctgacagatt
      121 tgtgcaggcg gccaaagatg agaaagccga tgctgtcgga atgtctgcgc ttttgactac
      181 cactatgact ggtatgaaag atgtgataga gctgatgaaa tccgaaggga tttatggcaa
      241 aataaaggtg ataataggag gcgctcctgt taatagagaa tttgcggaac aaatcggcgc
      301 cgattcctat gcatatgatg gcatgaaggc ggtggaaatt ctcaaggaat atgtgaaaga
      361 taaatagtat gagagacttt ttatatagaa taagagccgg tgagattatt gtcgcggacg
      421 gcgccatggg cacgatgtgc tttgaagcgg ggctgagagt tggtgattgc ccggagcttc
      481 tgaatatcga aaatccgcag ttgatcgaag atattaccag gtcttatctt gaagccggtt
      541 cccagattgt ccagacgaat acattcggcg gcactgcgct caagctggct ccgtatggac
      601 tccagaagcg ttccgcggag attaacgaag cggctgtgaa gattgcgcgc cgggcggcag
      661 gtgatagagc ctatatctca ggctcatgcg gaccctcggg aaaaatcctt caaccctatg
      721 gcgataccga gccggagatc atctatgcct cgtttagaga gcaaataaat gccctgattg
      781 aggccggggc cgatattatt accgtcgaga caatggttga cctggaggag gccaaactgg
      841 ccattaaagc ggcacgccaa atatcatcga caattccagt gatggctgcg atgaccttcg
      901 attctacacc caatggattt ttcaccatta tgggcatatc gattgaagac gcggcggtcg
      961 gattaaaaga ggctggtgca gatattatcg gctccaactg cggcaatggt atccgggata
     1021 tggtcatgat agcctccggg ttcaggcagg ctaccgatat gccattgata attcagtcaa
     1081 atgctggttt gcctaaagtc gaaaatggaa agactgtata tacagaaacc ccggaaatga
     1141 tggccgaaca tgcgaaagat ctgatggtat taccagtttc agtaattggt ggatgttgcg
     1201 gcaccacgcc cgagcatatc agggccctgc ggaagatggt ggattcatgg gcagcctgat
     1261 tggacgcttt ctgacattgg ctggggcggc gatagtggta atcagcctct ttacgccggt
     1321 aattcaaatg gagtttgagg ttccggaaca gagactgggg cagattgtcc tcaagcctcg
     1381 gcctgtcatg gttgaatctt ctctttacga gctgggagat aagctgtcgg atttggatgg
     1441 ttcaggcgcg gagctgcccg aagtgctgga ctatctctgg ttgatctttc tcggcctcgc
     1501 ggtcataaac gggctgctcg caatcaagcc gtcattcccc cgaatattaa gagcgctggt
     1561 tggcatcctg cccctggcat tgctggtaat tgtgatctgg cagacagtat caaatccaaa
     1621 tgtagcaatt ggatatgccg atttgttaaa ctattttgtg aagggattct acgttcttct
     1681 ggccggaacc ctgattatat ttctcggcac tttaatgacc aaaggcaaac gaatacattc
     1741 ctccaggcct tcctgatgtg acgctttttt tcaaaaatgc ttgaaataag tccacatttt
     1801 ccattatatt tgcagaccga cgggagatag tgtatcgaga acaatataag ataaatattg
     1861 ttatcattag taaatgattt acatggtgga gcggtatatc taaactatgg ctttaacatt
     1921 caatcctata cctgcctctt accgcaggct gtctgacgat gagctgaccg agcggattag
     1981 aaggaagaaa caggcatcag gtgataaact ggttatcctg actcatcatt accagcgcaa
     2041 agaagttgca gccctgggcg actatatcgg cgattcgtat gctctctcga aaattgcctc
     2101 cgagctcgaa gccgaaatca tcgtattttg cggcgtgcat tttatggccg aggcagcgga
     2161 tattctatcc acagataaac agactgtata tttgcccaat ccgttagcgg gatgtcccat
     2221 ggctgacatg gctgccatca cgaatgttat gcatgcctgg gatcaattga aggagctttt
     2281 accggacacg aagataatcc caattgccta tatgaatacc gcggccgatc tcaaagcttt
     2341 ctgcggaaga aatgacggcc tcatatgcac ttcctccaat gccaccgggg cattcaggtg
     2401 gggtcttgaa agaggggaca aggtgttctt ctacccggat gaacacctgg gtagaaatac
     2461 ggccaataaa ttggctatcc ctgaaaatga aatagctgtc tgggactatc ggttgcgccg
     2521 actgggagga aatacccccg aaaaaataaa aaacgcaaag gtcatcatct ggaagggcta
     2581 ctgccatgtc cacaccacat ttacaccgga acatgtacag cgcatgcggg agaaattccc
     2641 cgatgttaaa gtcgtggtac acccggaatg tcctcaggaa gttgtaaagc ttgccgatgc
     2701 cgatggttct acagcattta tagtcaagta tgtggaggaa gctgagccgg gggcgaccat
     2761 tgcgattggc accgagataa atctggtcga ccgcctggcc catgaaaatc ctgacaagaa
     2821 aataatggaa ctgtccggaa atacctgcgc catgtgtgtc aatatgtacc gcaccaccct
     2881 caatgatctg gcctggtgcc ttgagaatct ggacgatatc aggccgatcg aagtccgccc
     2941 ggaaataaaa gaagaggcgc gtctgtcatt gtcccgcatg ctggaagtcc ccaggtagtc
     3001 caatttactg atattgttat ctctacatag c
//
LOCUS       DSEB01000309            1540 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1018043, whole genome shotgun sequence.
ACCESSION   DSEB01000309 DSEB01000000
VERSION     DSEB01000309.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1018043"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..>1540
                     /gene="thrS"
                     /locus_tag="ENO07_05620"
     CDS             <1..>1540
                     /gene="thrS"
                     /locus_tag="ENO07_05620"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDS01481.1"
                     /translation="IKEDHPFKRVELPLEEARKYYEERDAIYKEEILDEIEDDTVTLY
                     QQDSFTDLCRGPHLPSTGKVKAFKLLSSSGAYWRGDERNKMLQRIYGVSYPSKKQLDE
                     YLNMLEEAKKRDHRKLGRELDLFSINENIGSGLVLWHPKGAVIRNIIEEHWKKEHIDN
                     GFQLVFTPHIARLKLWEISGHTGFYAENMFQPNEVEGDLYQIKPMNCPFHIEIYRSAI
                     RSYRDLPIKYAELGTDYRYERSGVLHGLMRVRGFTMDDAHIFCTPEQLHQQILEVCQM
                     SLRFLKMFGFEDYKIYLSTRPEKYVGEAERWETAQDSLAKALDELGLEYDMDEGGGAF
                     YGPKIDIKIKDTIGRLWQCSTIQFDFNLPDRFGIYYIDRDNTQKRPYMVHRAILGSLE
                     RFFGVLIENYGGAFPLWLAPVQVKIMTITDNQNDYAKKIAEELKKHDIRCELDLRSEK
                     IGFKVREAETSKTPYMFVIGQKEVDDNKVSVRHYGQVDLGSMNLEEIISRIKEEIENK
                     YPRM"
BASE COUNT          430 a          341 c          409 g          360 t
ORIGIN      
        1 tcattaagga ggatcatccc ttcaaaaggg tcgaactccc cctcgaagag gccaggaaat
       61 actatgagga aagggatgcc atttataaag aggagatcct ggacgagatc gaggatgata
      121 cggtcaccct ttatcagcag gattcattca ccgacctgtg ccgcggcccg catcttccat
      181 caaccggtaa ggtcaaggca ttcaagctct taagctcctc cggcgcatac tggcgtgggg
      241 atgagcgcaa taagatgctg cagcgcatct acggcgtttc atatccttcc aagaagcagc
      301 tcgacgaata tctcaacatg cttgaggagg ccaaaaaacg cgatcacaga aagctgggaa
      361 gggagctcga tctcttttcg atcaatgaga atatcggcag cggcctggtg ttatggcatc
      421 ccaaaggagc cgtaatccgc aatatcatcg aggaacactg gaaaaaggaa catatcgata
      481 acggtttcca gctggtgttt actccccata tagcgcgcct gaaactgtgg gagatttcgg
      541 ggcataccgg tttctatgcc gaaaatatgt ttcagccgaa tgaggttgag ggcgacctgt
      601 accagatcaa gccgatgaac tgccctttcc atatcgagat ttacaggagc gcaatcaggt
      661 cttaccgcga tctcccgatc aaatatgccg aactgggcac ggactaccgt tacgagcgtt
      721 ccggcgtact tcacgggctc atgcgtgtgc gcggctttac catggacgat gcccatattt
      781 tctgtactcc ggaacagctt catcagcaga tattggaggt ctgccagatg tctttgagat
      841 tcctgaagat gtttgggttc gaggattaca agatttatct ctccacccgg ccggagaaat
      901 atgtgggcga agccgagaga tgggaaaccg cgcaggattc gctggcgaag gcgcttgatg
      961 agctgggact cgaatacgat atggatgagg gcgggggagc cttctatggc cccaagatcg
     1021 acataaaaat taaggatacg atcggaagat tatggcaatg ttctacaatt caatttgatt
     1081 ttaacctacc ggatcgcttt ggaatttatt atattgacag ggataatacg cagaagaggc
     1141 cctatatggt acatcgggcg atcctgggat cgctggaacg attcttcggc gtattgatag
     1201 aaaattacgg cggagcgttt cctctctggc tggcgcctgt gcaggtcaag atcatgacca
     1261 tcacggataa tcaaaatgat tatgccaaaa agattgctga ggaattgaag aaacacgaca
     1321 tccgctgtga acttgatttg aggtcagaaa agatcggctt taaagtccgt gaggcggaaa
     1381 catcaaagac cccctatatg tttgtaatag ggcagaagga agtcgatgac aataaggtct
     1441 ctgttcgtca ctatggacag gtagacctcg gttctatgaa tttggaggag ataatctcca
     1501 ggatcaaaga ggagattgag aacaagtatc caaggatgaa
//
LOCUS       DSEB01000310            5014 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001302, whole genome shotgun sequence.
ACCESSION   DSEB01000310 DSEB01000000
VERSION     DSEB01000310.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001302"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(406..1266)
                     /locus_tag="ENO07_05625"
     CDS             complement(406..1266)
                     /locus_tag="ENO07_05625"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDS01482.1"
                     /translation="MKFEFIQTHRKEYRISRMCEVLGVSNSGYYDWLHRPESARSREN
                     RRLTAKIRQSHQRSRGIYGSPKIYRDLKADGETCSENRVARLMKVDGIQSKMAPRFVI
                     TTDSKNTSQPAPDRLQRQFTVTRQDMAWVSDTTFIATREGWLYLAVILELFSRQVIGW
                     AMGNRNNSELVQEALTMALWRRGKVRDVIVHSDQGSTYASGDYQRQLQDNHLQCSMSR
                     KGECLDNAVAESFFGTLKNELVYHEDYRTRAQAKQSLFEYIEVFYNRQRRHASLDYRT
                     PVDFEEQYAC"
     gene            complement(1263..1571)
                     /locus_tag="ENO07_05630"
     CDS             complement(1263..1571)
                     /locus_tag="ENO07_05630"
                     /inference="COORDINATES: protein motif:HMM:PF01527.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDS01483.1"
                     /translation="MTKQRKPYKTYTREFKLEALRLMESSGRSASEIAMQLGIRRNQL
                     YKWKEQMTQKGDVPTAKRGRPRKEEQSETARLRQENKRLTEENDILKKAAVFFAKEAK
                     "
     gene            1915..2340
                     /locus_tag="ENO07_05635"
     CDS             1915..2340
                     /locus_tag="ENO07_05635"
                     /inference="COORDINATES: protein motif:HMM:PF12568.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDS01484.1"
                     /translation="MIKIREAEQIELGHLAEIYSAARVMAGCFSKPAADAQQLKRLVE
                     GEEIFVAELDGEVVGFVSVWVADNFIRHLYVAPAAQGKGVGSRLLQFCTREYGHPLSL
                     KCEAQNVQAQRFYRNKGWFDREIGEGDDGVWERLYSKNA"
     gene            2518..3186
                     /locus_tag="ENO07_05640"
     CDS             2518..3186
                     /locus_tag="ENO07_05640"
                     /inference="COORDINATES: protein motif:HMM:PF14094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4272 domain-containing protein"
                     /protein_id="HDS01485.1"
                     /translation="MFCANCDNEIEPENSLCPLCGFDLTHIIALLNESDDDCADIEDE
                     VRAPGEIAKRALSLAAVISCAYGDSKKDVVAWLKKENLWDAVSPSEKEFLENETTKEQ
                     NAKFTWRIEALVPLLWSINKIGKMPGIKSECNTEPLKKAVIWPPNSTKEYIASSKIRA
                     EDELFEEYEKVYQAHWKVRDAQLNNKPVPGKYNPEVVYERHYGFNWLTGYMGQDWDDI
                     TTDT"
     gene            3260..3718
                     /locus_tag="ENO07_05645"
     CDS             3260..3718
                     /locus_tag="ENO07_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HDS01486.1"
                     /translation="MLPSELEHYLHEHIPLSKAMAVSVVVAGPEEVVLSAPLTPNINH
                     RETVFGGSASALATLAAWSLLHIRLRAEGFSSRLVIQRNTMDYDLPIDGTFTARASVD
                     QVSDWSRFVRMLSRRGKARVAVASVLEYNGQVAGRFSGEFVAFGGNRDNG"
     gene            3739..4206
                     /locus_tag="ENO07_05650"
     CDS             3739..4206
                     /locus_tag="ENO07_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01487.1"
                     /translation="MDVSIPTVLKALSSAGTAMKEFGAWKARARGDERALIAELKDNL
                     HYLDLVAEDGVLLREVVGKLSVAEYKKLANRGFNFDTIKKKPIENYPSLAETDLAAWP
                     GKHTGELIESIYDKINELKIKFPYVENNKHYRWNARVNNIRKRIWLLLRHVRG"
     gene            4218..4997
                     /locus_tag="ENO07_05655"
     CDS             4218..4997
                     /locus_tag="ENO07_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01488.1"
                     /translation="MSLKLYLLIPGCSNGFAGVLISLFLTVTSTVLADGPVATFKSAM
                     QHTVTGDLYVSTGYPVNHDGYEAALDRLWEKAGDKLPRDSYRQEWKQIEPGELRSVFG
                     HFDTQYYLLFPIVKRVDSGNGYGRIEFAGYAIERREVTGAALFRWGCGGEVIPVLNLG
                     PWPSDTYREWGPLAISIHGKFGEARLVELDKSLVPEAYARQVANTEQWVTIEWPDVKY
                     RATVTHHTFATGEASQHIFATGDSPGYFSFLEPRWPMDYRC"
BASE COUNT         1230 a         1123 c         1387 g         1274 t
ORIGIN      
        1 acagatggta ttgaaataca aaaagggcga ttttcgaatg tttttgcatg gaataaaatt
       61 ggggatatca agagcagcga aatatttcag gttttaaaga tatatgatct tgctggagag
      121 ttgatttacg cggtcgatta ttacgcggat aactttggca agttttataa gacaatcaat
      181 gaagtatatg ggtaaatcct gataagcaac gccgaatccc gaaggcagta tatctgttta
      241 cagggttgag ggcgaaacag gcctcaagcc ttaacaaggg aatgtcagtg ctagcgaacg
      301 aacttgaaga ttatctccac gaacacatcc cgctgtccag ggcgatgggg gtgtccaagg
      361 aaaaggggac ggagtgaggt tcccccaata aaacggacga tttaattagc aggcgtactg
      421 ctcctcaaag tctaccggtg tccgatagtc aagtgaagca tgacggcgct gccggttata
      481 aaatacttcg atatattcaa acaggctttg tttggcctgg gccctcgtcc ggtagtcttc
      541 gtggtagacc agttcattct ttagcgtgcc aaagaagctc tcggccacag cgttgtccag
      601 gcattcgcct ttgcgactca tgctgcactg caagtgatta tcctgtaact ggcgctgata
      661 gtcaccggag gcataggtac tcccctggtc cgagtggaca atgacatccc tcactttacc
      721 tcgacgccag agcgccatgg tcagcgcctc ctggaccagt tcactgttgt tgcggttgcc
      781 catcgcccag ccaatgactt gccgtgagaa cagctccagg attaccgcca ggtataacca
      841 gccttcacgg gttgcaataa aggtggtgtc cgacacccag gccatatcct gtcgcgtaac
      901 agtgaattgg cgctgcagtc ggtcgggcgc cggttgtgat gtgtttttcg agtcggtggt
      961 aatcacaaat cgcggcgcca tttttgactg tatcccgtcg accttcatca gccgcgccac
     1021 ccggttctcg ctgcaggttt cgccgtcggc tttcagatcc cgatagattt tcggggaacc
     1081 gtagatcccc cgggagcgtt gatgggactg ccggattttc gccgtgagtc ggcgattctc
     1141 ccggctgcgc gcactctcgg ggcggtggag ccagtcataa tacccactat tcgagacgcc
     1201 caatacttca cacatgcgac tgattctgta ttctttgcga tgcgtttgaa tgaattcaaa
     1261 cttcatttgg cttccttcgc aaagaatacg gcggcctttt ttaggatgtc attctcctct
     1321 gtcaggcgtt tattttcttg ccgcaagcga gccgtttcac tttgctcttc tttcctgggc
     1381 cggcctcgct tggccgtggg gacatcacct ttttgagtca tttgttcttt ccacttgtaa
     1441 agttggttgc ggcggatacc taactgcatg gcaatctcgc tggctgaacg gcccgaggac
     1501 tccatcagtc gcagagcttc cagtttaaac tctcgtgtat aggtcttgta gggctttcgt
     1561 tgtttggtca ttgggacact ccttccggga gaccattgcc tccgatttaa agtgtccgtt
     1621 aaactggggg aggttcagag ggattaaaaa gtgagcaatc tgaaaaggtg gttaaaatta
     1681 aatccctccg tcccctttac gggtgattta attcccaagc tgaaagggta ccgtcttatt
     1741 tatgcatagg gctgaaagcg tccgaattaa aatcaaaatg gtatttcgag tgatctggcc
     1801 ccttgattct gccttgattc cgcggagact cgtgttaata aatctaacaa acacatctat
     1861 taggacactc gcaagctcgc gcctattacg taagtgttat gcatcacatt gagcatgatc
     1921 aaaatacgag aagctgaaca gatagagcta ggtcatctgg cagagattta tagcgctgct
     1981 cgggttatgg cgggttgttt ctcaaagcct gctgctgatg cgcagcagct taagcgtttg
     2041 gtggagggcg aggaaatatt tgttgctgaa ctcgatggcg aagtcgtagg ttttgtctct
     2101 gtctgggtgg ccgataactt tatacgtcat ttatatgtag cgccagccgc gcaaggaaaa
     2161 ggtgtaggta gtagattatt acagttttgt acgcgagaat acggtcatcc attgtcattg
     2221 aagtgtgaag cacaaaatgt ccaggctcaa cggttttatc ggaataaggg atggtttgac
     2281 agggaaattg gagaaggcga tgacggggta tgggagaggc tgtattcaaa aaatgcataa
     2341 caaaccggtc aaaacaaaca caatagactc cgcacaattt gcgcatagta aaattggcat
     2401 ctgagaccaa ctgtttataa tattagagat cattcgtctc tgacgcctgt ttcttgtatt
     2461 ttcaagagtc aatcttttgg cagtaaacgt cactgtcgga tgcaataagg aaggattatg
     2521 ttttgtgcta attgcgacaa tgaaatagaa cccgagaatt ctttatgtcc tctttgcggt
     2581 tttgatctca cgcacataat tgcattattg aatgaatctg atgatgattg tgctgatatc
     2641 gaagatgaag tgagagcgcc gggcgagata gcgaaaaggg cgctgtcgct tgcagcggta
     2701 atttcatgtg cgtatgggga ttctaaaaaa gatgttgtgg cctggttaaa aaaagaaaat
     2761 ctttgggatg ctgtttcacc ttctgagaaa gaatttttgg aaaatgaaac cactaaagaa
     2821 caaaatgcca aattcacgtg gagaatagag gcgcttgtac cacttttatg gtcaattaat
     2881 aaaatcggaa aaatgcctgg cataaaatcg gagtgtaata cagagccatt aaaaaaagct
     2941 gtaatctggc cgccaaactc cacaaaagaa tacattgcct catcaaaaat tcgagcagaa
     3001 gatgagctgt ttgaagagta tgagaaagtc tatcaagcac attggaaagt aagagatgcg
     3061 caattaaata acaaaccggt gcctggaaaa tacaacccgg aagtcgttta tgagcgtcac
     3121 tatggtttca actggcttac tggctatatg gggcaagatt gggatgatat cacaactgac
     3181 acctagggaa tttcgggggc agtatacctg tcccaaatac tgaggatggt acaagaccaa
     3241 agcaacatag aaaatacaga tgcttccgag cgaacttgag cactatctcc acgaacacat
     3301 cccgctgtcg aaagcaatgg cggtgtcggt ggttgtcgcg gggccggagg aggtggtatt
     3361 gagtgcgcca ctgacgccga atattaatca tcgcgagacg gtgttcgggg gcagtgcctc
     3421 ggcactggcg acgctggcgg cctggtcctt gttgcatatc cggcttcggg cggagggctt
     3481 tagcagccgc ctggtgatcc agcgcaatac gatggactat gatttgccga ttgacgggac
     3541 gttcacggcg cgggcatcag tggatcaggt atcggactgg tcgcggtttg tgcggatgct
     3601 gtcgcgccgg ggaaaggccc gggtggcagt cgcatcggtg ctggagtata acgggcaggt
     3661 ggcggggcgg ttctctggcg aatttgtcgc ttttggggga aatcgtgaca atggttgatt
     3721 ggacaggttg aacgatccat ggacgtgagt attccgacgg ttctcaaggc gctatcctcg
     3781 gccgggacgg cgatgaagga gttcggcgcc tggaaggcgc gggcgcgcgg ggatgaacgg
     3841 gcgttgatcg cggagctgaa ggataatctg cattatctgg atctggtggc ggaagacggt
     3901 gtgctgttgc gggaggtggt cggcaagctc tcggtggcgg aatacaaaaa actggccaat
     3961 cgcggcttca atttcgacac catcaagaaa aagccgatcg agaactatcc gtcactggcg
     4021 gagacggatc tggctgcctg gccgggcaag catactggcg agttgatcga gtcgatctac
     4081 gacaagatca acgagttgaa aatcaagttt ccctacgttg aaaataacaa gcactaccgc
     4141 tggaacgccc gggtgaacaa catccgcaag cggatctggc tgttgctcag gcacgtgcgc
     4201 ggctgaggag gcagagaatg tcattgaagc tgtatttact tattcctggt tgcagtaacg
     4261 ggtttgccgg ggtattgatt tcgctgtttt tgaccgtcac ttcgaccgtg ctggcggatg
     4321 gcccggttgc aaccttcaag tctgccatgc agcacacggt taccggcgat ctctatgttt
     4381 caaccggtta cccggttaac cacgacggtt atgaggccgc gcttgatcgg ttatgggaaa
     4441 aagcgggcga taaactgccc agggacagtt accgtcagga atggaagcaa atagaacctg
     4501 gtgaattacg atcagtattt ggccatttcg acacacaata ctatttattg tttccgatag
     4561 ttaaacgggt cgattcgggt aatggttacg gccgtatcga gtttgccggt tatgcaatcg
     4621 agcgccgtga ggttaccggt gccgccctgt tccgctgggg gtgtggtggc gaggtgattc
     4681 cggtgctcaa cctcggaccc tggccgtcgg acacctaccg tgagtggggg ccgctggcaa
     4741 tatccataca tggcaagttc ggtgaggccc ggctggtcga gctggacaag agcctggttc
     4801 ctgaagcata cgcccggcag gttgctaaca ccgaacaatg ggtgacgatc gagtggccgg
     4861 atgtgaaata ccgtgcaaca gttactcacc ataccttcgc gactggcgaa gcctcccagc
     4921 atatttttgc caccggcgat tcacccggtt atttttcctt tctcgaaccg cgctggccga
     4981 tggattatcg ttgctagtct tgtttgagtt ccgc
//
LOCUS       DSEB01000311            3877 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002318, whole genome shotgun sequence.
ACCESSION   DSEB01000311 DSEB01000000
VERSION     DSEB01000311.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002318"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            101..3535
                     /locus_tag="ENO07_05660"
     CDS             101..3535
                     /locus_tag="ENO07_05660"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDS01489.1"
                     /translation="MSKADFIHLHNHTQYSLLDGACHIDKFLELARELNFPALAITDH
                     GNMFGAIEFYKKARNKGIKPIIGCEVYVAPQDRFKKERLPGEPDGGYHLILLAKNNAG
                     YKNLIKLVSAGYLEGFYRKPRVDKELLRQYSEGLIATSACLQGEISRKIANNDFKKAE
                     EIARQYADMFGQENFYLEIQDHGIDKEKKDKAALTKIARKLGFGLVATNDCHYLHRED
                     HNAHEALLCIQTGKSLDDTDRMSYATDQIYVKSADEMKALFADTPEAIENTLKIAEQC
                     NVDIEIGKLMIPKFDLPKDFDNPEDYVRHLCEEGLKKLYDDPGDKIYERLNYELDVIK
                     KMGFSGYFLIVKDFIDYARSQNIPVGPGRGSAAGSLVSYTLGITTIDPLKYDLLFERF
                     LNPERVSMPDIDIDFSDRGRDKVIDYVTNKYGQDNVSQIITFGTLAARGVVRDVGRVM
                     GIPYAEVDKIAKMIPFKVGQTIAGAMEEEPDIGRLAKTDEKVSTLLEYSMTLEGLARH
                     TSIHAAGVVIAPEPLVEHVPLFKSNKDEITTQYDMKGVEDIGLLKMDFLGLRTLTVIA
                     DTVQMIKEKHGVDIDLDKVPLDDEKVYELFGNGNTIGIFQFESSGMQDYLKKLHPESL
                     DDLAAMNALYRPGPLDSGVINLYIDRKQGREKIEYLHPDMEPILKNTFGVIVFQEQVL
                     QLASKLAGFSLGKADILRKAMGKKIAKLMAQMKKDFINGCVENGISKKMAREIFHQIE
                     KFARYGFNKSHSVGYAYLAYQTAYLKAHYPREFMAALMTSEMGNTDRIIILKEECRNM
                     EIEILPPDINRSYANFNVEGDSIRFGLAAIKNVGMNAVSKIIEEREANGEFKSIFDFT
                     SRCDLSSINKRTIESMVMAGALDSVGKDRGLLYASVEDAVSFGQSVQIDRLKGQTSLF
                     GEAENNILPNPEPRISEEGSWSRSKVLSMEREALGFYISGHPMEKYRPQLKLFATTNS
                     EKMNDMPDGVQAALGGIITRLKINTDKRGRHMGFAGVEDFYGSFEAIVFSDPFEKYKK
                     LLINNNMLLFTGRISKREEEKPKLIVNELRPLESLSQNSNLLLKLTVSGRRIKKVEEI
                     KRILDKFPGEAKVIILLKQNDITMEIATSQQVNPNGELMESLSEILNSENLSLSAG"
     gene            3640..>3877
                     /locus_tag="ENO07_05665"
     CDS             3640..>3877
                     /locus_tag="ENO07_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01490.1"
                     /translation="MKLRLLLAFGIILLAGIFVIAQAGDEKEENAGVTYYGYEEGLEK
                     AKQDSLHVVVFFETTWCTWCKKMDQTTLVDDDVVG"
BASE COUNT         1104 a          867 c         1012 g          894 t
ORIGIN      
        1 cggaaatatg aaaaggcgat tgaacttgat cccgacaacc aggaatataa acagatctat
       61 gacgacgtac tacaagcttt agaggaattg gaacaacaaa gtgagtaagg ccgacttcat
      121 acatttacat aatcataccc agtattctct actggacggc gcatgtcata tagacaagtt
      181 ccttgagctc gcccgggaac ttaactttcc ggcactggcc atcaccgatc acggcaatat
      241 gttcggcgcg atcgagtttt ataaaaaagc caggaataaa ggaatcaagc cgattatcgg
      301 ttgcgaggtc tatgtggcgc cgcaggacag gttcaaaaaa gaacgtctgc ccggcgagcc
      361 ggacggcggg tatcatctga tacttttggc caagaacaat gccggctaca aaaacctgat
      421 aaagctggtt tccgccggtt acctggaggg attctaccgc aagccgcgtg tggacaagga
      481 actgcttcgc cagtatagtg aggggctgat tgccacctcc gcctgcctgc agggtgaaat
      541 ctcgaggaaa attgccaaca atgatttcaa gaaggcggaa gaaattgccc gccagtacgc
      601 cgatatgttc ggccaggaaa atttctacct tgaaatccag gatcatggaa tcgataaaga
      661 gaaaaaggat aaggcggctt taacgaaaat cgccagaaaa ctcggctttg gcctggttgc
      721 caccaatgac tgccactacc tgcaccggga ggatcacaac gcacatgagg cgctgctatg
      781 catacagaca ggcaaatccc tggatgacac cgaccgaatg agctacgcca cagatcagat
      841 ctatgtcaaa tcggctgatg aaatgaaggc ccttttcgca gatacaccgg aggcaatcga
      901 aaatacactc aagatagctg agcagtgtaa tgtcgatatc gaaatcggca agctgatgat
      961 cccgaaattt gatttgccga aagatttcga caatcccgag gattatgtcc ggcatctctg
     1021 tgaggaggga cttaagaagc tctatgatga cccgggagat aaaatctatg aacggctgaa
     1081 ctatgagctg gatgtgatca aaaagatggg tttttccgga tacttcctga tcgtgaagga
     1141 tttcatcgac tacgcccgct cgcagaatat tccggtgggg ccgggacgcg gttcggccgc
     1201 cggcagcctg gtttcgtata cgctcgggat taccacaatc gatccgttga agtatgatct
     1261 tctcttcgag cgttttctaa atccggaacg tgtgagtatg cccgatatcg atatcgattt
     1321 ctccgaccgc ggccgggaca aggtgattga ttatgtaacc aacaagtatg gtcaggataa
     1381 tgtatcacag attatcacgt tcggcactct ggcggcgcgg ggagtagtaa gagatgttgg
     1441 acgggtgatg gggattccct atgcggaagt ggacaaaatc gctaagatga tccccttcaa
     1501 agtcgggcag acaatagcag gggctatgga ggaagaacct gatattggac ggcttgccaa
     1561 gaccgatgaa aaagtcagta cgctcctgga atattccatg accctggagg gactggcaag
     1621 gcacacctcc atccacgccg ctggagtagt gattgcacct gagccgctgg tggaacatgt
     1681 tccactcttc aagagcaaca aggacgaaat caccacccag tacgatatga aaggtgtcga
     1741 ggatatcggc cttttgaaaa tggatttcct gggtctgaga acattgactg ttattgccga
     1801 tacggtccag atgatcaaag agaaacacgg tgtcgatatt gatctggaca aagtccctct
     1861 ggacgacgaa aaagtatacg agctttttgg caatggcaat acaatcggga tcttccagtt
     1921 tgaatcatcc ggtatgcagg actatttgaa gaagcttcat cccgaatctc tcgatgatct
     1981 ggcggccatg aatgccctct atcggcccgg accgctggat tcaggcgtga tcaacttata
     2041 tatcgaccgc aagcagggcc gcgaaaaaat cgaatatttg catcccgata tggagccgat
     2101 cctcaagaac acctttggcg tgatcgtatt ccaggagcag gttctgcagc tggcctccaa
     2161 acttgccgga ttcagccttg gaaaggcaga cattctcaga aaagctatgg gcaagaaaat
     2221 agcgaagttg atggcgcaga tgaagaagga tttcatcaac ggctgcgtgg agaacggtat
     2281 ctcaaagaag atggccaggg aaatatttca tcagatagag aagtttgccc gctacggctt
     2341 caacaaatcc cattcggtcg gctatgctta tctggcttat caaactgcat atttgaaggc
     2401 gcactacccc agggaattca tggcggcctt gatgacgtcc gagatgggca acacagaccg
     2461 gatcataata ctcaaggaag aatgccgtaa catggaaatc gaaatcctcc cgcccgatat
     2521 caatcgcagt tacgccaatt tcaatgtcga gggggattca atccggttcg ggctggcggc
     2581 tatcaagaat gtgggtatga atgcggtttc aaaaattatc gaggaacgtg aggcgaatgg
     2641 cgaatttaaa tcgatctttg atttcacctc acgctgtgat ctgtcctcga tcaataaacg
     2701 cacgattgag tcgatggtga tggccggggc tttagactca gtgggcaaag atcgcggttt
     2761 attatatgct tcggttgagg atgcggtctc atttggccaa tcggtgcaga tagaccgcct
     2821 caagggccag acctcacttt tcggggaggc cgaaaacaac atcctgccga atcccgagcc
     2881 ccggatttcc gaggagggca gctggagccg ctccaaagtt ttatcaatgg agcgagaagc
     2941 gctgggattc tatatttccg gccatccaat ggagaaatac cgtccccagc tcaaactctt
     3001 cgctaccacg aattcggaga agatgaatga tatgcctgac ggcgtgcaag ccgcgctggg
     3061 gggaattata acccggctca aaatcaacac cgacaagcgc gggcggcata tgggatttgc
     3121 aggggtggaa gatttttatg gaagcttcga agccatagtc ttttccgatc cgttcgagaa
     3181 atacaagaaa ctgctgatta acaacaacat gcttctattt accgggcgca taagcaagcg
     3241 cgaagaggaa aagcccaagc tgattgtaaa tgagctcaga ccgctggagt cattgtcgca
     3301 gaattcgaac ctgctcctca agctaaccgt ctcgggcaga aggattaaga aagttgagga
     3361 aatcaagagg atcctggata aatttccggg tgaagcaaaa gtaattatcc ttctgaagca
     3421 aaatgatatc acgatggaaa ttgcaacgtc ccaacaggtt aatccgaacg gtgaattgat
     3481 ggaatccctt tctgagattc tgaactctga aaatctgtcc ctttcagccg gataagggaa
     3541 ggaactcata tcggttaaca gtgttaaagt agaaagaatt tgatttttta cgatttttgt
     3601 atagattaag aaaaatgtta catcagaccg ggagtgccca tgaaacttag acttttgctc
     3661 gcttttggaa ttatactttt ggccgggata ttcgtgattg cccaggctgg tgatgaaaaa
     3721 gaagaaaacg ccggtgtgac ttattacggg tatgaggagg gcctcgaaaa agccaaacag
     3781 gattctctgc atgtggtagt gttttttgaa accacatggt gcacctggtg caagaaaatg
     3841 gaccagacta ctctggtgga tgacgatgtt gtggggc
//
LOCUS       DSEB01000312            2101 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1009054, whole genome shotgun sequence.
ACCESSION   DSEB01000312 DSEB01000000
VERSION     DSEB01000312.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1009054"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..925
                     /locus_tag="ENO07_05670"
     CDS             <1..925
                     /locus_tag="ENO07_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDS01491.1"
                     /translation="SFYTMGINDLLKEAGSQIPGITKILSVHKDDQIKKPIAFNIDYE
                     NDDLIVTFDGLVSDGKFIPQVRTLMKLLEKELDRPVDIEFAHDGKNFYLLQCRPQSYV
                     EQDTGDKIPREIEPDDVIFSAHKYISNGKVPEITHIVYVDPGGYADINELNELLAVGR
                     VVNKLNKLLPEHGFILMGPGRWGSRGDIKLGVNVTYSDINNTAMLIEIARQKGNYVPD
                     LSFGTHFFQDLVESNIRYLPLYPDDPGIKFNEDFLLNSPNLLPELLPDYAHLSECVHV
                     IDVPAVTGGKVLRVYLNADQREGVGVLEDPR"
     gene            complement(1282..>2101)
                     /locus_tag="ENO07_05675"
     CDS             complement(1282..>2101)
                     /locus_tag="ENO07_05675"
                     /inference="COORDINATES: protein motif:HMM:PF16138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01492.1"
                     /translation="GITIIVVACSLSWAQDNPYPWLEEYDSSDAIVQRIDVPDGYKRV
                     SPMEGTFAHWLQHLPLKQDGSTVYLYNGDKKADQNYHYAIINIDRSEQDLMQCADAII
                     RLRAEYLYSIGEFDMIHFNFTSGHEAKYTSWIKGYRPKVDEMRVNWVKNAPLDSSYES
                     FREYLDSVFMYAGTYSLDNELEKVEDVSKMRVGDVFIKPGFPGHALIVVDMAVDKDNG
                     RSLFLIAQGYTPAQDIHIIKNLHDEDLNPWYPLDFGDTLKIPEWEFPKEELKRF"
BASE COUNT          536 a          501 c          500 g          564 t
ORIGIN      
        1 cagtttctat acgatgggga tcaatgatct tttgaaagag gccggttctc aaatcccggg
       61 catcactaaa attctctcgg ttcacaaaga tgaccagata aagaagccga ttgcgttcaa
      121 tatcgattat gagaatgacg atcttatagt gactttcgac ggcctggtct ccgacggcaa
      181 gtttattccg caggtgcgta ctcttatgaa gctccttgag aaagagctcg acaggccggt
      241 ggatatcgaa ttcgcccatg acggtaaaaa tttctacctg ctccagtgca ggccgcagag
      301 ttatgtggaa caggataccg gcgacaaaat accgcgcgaa attgagcccg atgatgttat
      361 cttctccgcc cataaatata tttccaatgg taaggtgccg gagataaccc atattgtcta
      421 tgtcgatccg ggcgggtatg ctgatataaa tgaattgaat gagctgctgg ctgtgggacg
      481 agtggtcaat aagctgaata agctcttgcc ggaacatgga ttcattttga tgggtccggg
      541 gcgatggggc agccgcggcg atattaagct gggcgttaat gttacctatt ccgatatcaa
      601 taacactgcc atgttgatag agatcgcccg ccagaagggc aattacgtgc ccgacctctc
      661 attcggcacg cattttttcc aggatctggt ggaatcgaat atcagatatc ttccacttta
      721 tcctgatgac ccgggtatca agttcaatga ggattttctt ttgaattcgc ctaatctcct
      781 gcctgaactt ttgcccgact acgcccatct ttctgaatgc gtgcatgtca tcgatgttcc
      841 cgcagttacc ggcggcaagg ttctgcgggt ttacctcaac gccgaccagc gcgaaggcgt
      901 cggtgttttg gaagacccca ggtaaaatag ccaactcttt ggagcgggca atagtagatt
      961 tttcagtttt gaattgtttt gaaattatca gttggaaacc catggctaaa gcgagtttgt
     1021 tggaaaaacg cattcatcag tctgtgatcc tgagtcccga tttatcggga cgaaggatct
     1081 cgttaatgcg caagagattc ttcggtcact acgttccctc agaatgactg cagatagtag
     1141 tgctcttcat attccatatt ttctcaacat tcccctaaag cgaaggggca cctgcggacc
     1201 tgcccaacaa agcagacccg ccgagagcct ggtctctgag cgatttgtgg gcggcacaac
     1261 gtccgcgcat ttttatttga atcagaaccg cttcaattct tctttgggga attcccattc
     1321 agggattttg agggtgtcgc caaaatcgag cgggtaccat ggattcaaat cctcatcgtg
     1381 cagattcttt ataatatgga tatcttgagc aggcgtatat ccctgggcga taagaaacaa
     1441 actcctgccg ttatctttgt caaccgccat atcgaccaca atgagcgcat gcccgggaaa
     1501 acccggcttg atgaaaacat ctccgacacg cattttggaa acatcttcga ccttctcaag
     1561 ctcattatcc agagaataag ttccggcata cataaaaacc gaatccagat attcacggaa
     1621 gctttcatag gatgaatcga gcggggcgtt cttgacccag ttgacccgca tctcatcaac
     1681 tttggggcga tatcccttga tccagctggt gtattttgcc tcgtgtccgg aggtgaaatt
     1741 aaaatgaatc atgtcaaatt cgccaataga ataaaggtat tcagctctta acctgattat
     1801 ggcgtcagcg cactgcatca gatcctgctc gctgcggtcg atattgatta tggcgtaatg
     1861 ataattctga tcagctttct tatcaccatt atacaggtaa actgtcgagc catcctgctt
     1921 gagaggtaga tgctgcagcc agtgtgcaaa tgttccctcc atcgggctca cgcgtttgta
     1981 accgtctgga acgtcgatac gttgtacgat cgcgtccgag ctgtcatatt cctcaagcca
     2041 gggatacggg ttatcctgag cccagctgag agagcaggca accacaataa tggtaatacc
     2101 c
//
LOCUS       DSEB01000313            4325 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001831, whole genome shotgun sequence.
ACCESSION   DSEB01000313 DSEB01000000
VERSION     DSEB01000313.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001831"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            305..1582
                     /locus_tag="ENO07_05680"
     CDS             305..1582
                     /locus_tag="ENO07_05680"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HDS01493.1"
                     /translation="MKADLYIRHIGQLATCRPPDGKLPLKGSGMNQLEIIENGALACL
                     AGEIILVGTTADVDRQIDLVNRAVVIDADDKVVTPGLIDPHTHPVFAKTREDEFEMRN
                     MGKSYMEIAQAGGGIRNSTRALRETSKDALYDNAKKYLDLFLQYGTTTIEAKSGYGLT
                     TQDEIKQLEVIQMLSENHALDLIPTFLGAHEIPDEYRDDKDAYVELIINEMIPVVAER
                     NLAVFSDVFCEKGVFELEESERIQVAAKEHGLELKFHADEIVNLGGAQLAAKLGAVSA
                     DHLVYISEEGIAAMAEAGTMAVMLPGTSFYLDLRDYAPARRMIEAGVPLALSTDFNPG
                     SSATVSLQMIMTLAGNKLKMSTNEIINAVTINSACALKQEKNIGSIEPGKKADIVVWN
                     VSNLKQLPYFYAINQVEKTIKNGRVVYDSQGTK"
     gene            1610..2098
                     /locus_tag="ENO07_05685"
     CDS             1610..2098
                     /locus_tag="ENO07_05685"
                     /inference="COORDINATES: protein motif:HMM:PF04390.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01494.1"
                     /translation="MRVKNIILALLLISFLASCGPYSFSGATTGGIETVYIPVFENET
                     IEYGLGEELTTKITSAIVADNTLKVVDQNDADAFISGKVISYKKSSETYNIQDQVQEY
                     RVDVAVNVSFVKPNGDVIWEEPRISSFGVYEAETETEDEGKTEALEKLAEIIVNRTVR
                     NW"
     gene            2137..3084
                     /locus_tag="ENO07_05690"
     CDS             2137..3084
                     /locus_tag="ENO07_05690"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HDS01495.1"
                     /translation="MKNKFALSLLAALSLMIFTNANLSAINSKAGTAAYSFLKIGVGA
                     RAVSLGNSFVGLADDESAIYYNPAGMSRIPGYALSTSYHNYVADIQGGYLSAVLPWGD
                     GRKLGFAINYLSFGDTPETNNAGEVIGNFGGGDLALTAAYSQSLMEQLYLGLAGKFIY
                     ESIDSFHSTGMALDIGLLYESPDGYTSLGAVASNLGFQLSGLSEEHKDPLPIMFKLGV
                     AHQMHGAPLNIVADVGKFSDNDFFIGGGVEYVGIEAIRLRLGYNTVGSDYKTGGDSES
                     LAGLSFGLGINVSKLTFDYAYVPFADLGNSHRIYIARRW"
     gene            3103..3546
                     /locus_tag="ENO07_05695"
     CDS             3103..3546
                     /locus_tag="ENO07_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01496.1"
                     /translation="MKRALLIIAAAILALAIAACEKEPEARARSTVLDFVKELRSEGT
                     IELDNYLILNELVRENAENVYTYNDSLSVSENIEQFRELFEPNGRVRRLWTQKQIIVG
                     ESEVLGDTAYVEVSFIDREERRQFYNKMGLKRTDEGWKIFAFKLL"
     gene            3842..4090
                     /locus_tag="ENO07_05700"
     CDS             3842..4090
                     /locus_tag="ENO07_05700"
                     /inference="COORDINATES: protein motif:HMM:PF00313.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HDS01497.1"
                     /translation="MEFGTVTKLEKGHGYGFIQTEDKKEVFFHQRWLRYIKFRDINIG
                     SKVVFDLQKGHRGLKALNMRFAPDDENFLKNFTPRNRK"
     gene            4169..>4325
                     /locus_tag="ENO07_05705"
     CDS             4169..>4325
                     /locus_tag="ENO07_05705"
                     /inference="COORDINATES: protein motif:HMM:PF00929.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01498.1"
                     /translation="MALYRDMMQFCFIDTETTGLFTSYGDRICEIGLLAVDETLEIIE
                     SFESLVNP"
BASE COUNT         1178 a          937 c         1109 g         1101 t
ORIGIN      
        1 cttcttcatc cagtatatcg gatagggtct cgcatatgtc ctgatcatcg tcaacgatga
       61 gtatcttttt ggtttttgtt ttagttttca taatgcccca actggctatt taaatattct
      121 atcggactga gaaatgccgc ccttaatttt taaaatcgga ttcacgaatt tctaacattt
      181 taatcgtcag gttgattatg ccgcagaaaa ctgttgcaat cggccttaat tttcctaaat
      241 ttccgcatct acacccggca tttcaattat gccatcagaa aaatagccta agaaggatgg
      301 gcctatgaaa gcagatctct atatcaggca tatcggacag ctggcaacat gccgtcctcc
      361 cgatggcaag ctgcccctta aaggttctgg aatgaatcag cttgagatta ttgaaaacgg
      421 cgctcttgcc tgcctggcgg gagagatcat cctggtcggc acgaccgccg atgttgaccg
      481 gcagatagat ctggtcaacc gggctgttgt gatagatgct gatgacaagg ttgtaacgcc
      541 cggtctaatc gatccccaca cccatccggt cttcgccaaa acgcgcgaag atgaattcga
      601 gatgcgtaat atgggcaaat cgtatatgga aattgcccag gcggggggcg ggatcagaaa
      661 ctccacaagg gcgctgagag aaacgtcgaa ggatgcgctt tatgataatg cgaagaagta
      721 cctggattta ttcctccagt atggaacaac cacaattgaa gcgaaatccg ggtatggcct
      781 gaccacacag gatgagataa aacagctgga ggtgatccag atgctctccg aaaatcatgc
      841 cctggacctt atccccactt ttctgggggc gcatgagatt cctgatgaat accgcgatga
      901 caaagacgcc tacgtggaac tgattataaa tgagatgatt cctgtggttg cggagcgcaa
      961 tctcgccgtg ttttctgatg ttttctgcga aaagggtgta tttgagctgg aagagtcgga
     1021 aagaatccag gtggccgcca aagagcatgg tctggagctg aaatttcatg ccgatgagat
     1081 cgtgaatctg ggaggcgccc agcttgcagc gaaactgggg gcagtttcag ccgatcatct
     1141 ggtctatata tccgaggagg gaatcgcggc catggctgag gcggggacta tggcggttat
     1201 gcttcccgga acatcatttt acctcgattt gcgggattat gcccctgcca gacggatgat
     1261 cgaggccggg gtgccgctgg cgctttccac cgatttcaat ccgggaagtt cggccacggt
     1321 ctcgcttcag atgattatga cattggcagg caacaagtta aagatgagca ccaatgagat
     1381 catcaatgcc gtgacaatca attccgcctg tgccctgaag caggaaaaaa atatcgggtc
     1441 gatagagccc ggtaaaaaag ccgatattgt agtctggaac gtatcaaatt taaagcagct
     1501 tccctatttc tatgctatca atcaggtcga aaaaaccata aaaaatggaa gagtggtgta
     1561 cgattcccaa ggaacgaaat agtggaagcg gcactataat cattgaggta tccgggtgaa
     1621 aaatattatt ctcgcacttc ttctcatatc attcttagcc tcctgcgggc catattcatt
     1681 ctcaggcgca accacaggcg gaatcgagac ggtttatatt ccggtttttg aaaatgagac
     1741 tattgaatac ggcctgggcg aggagctgac aaccaagata acttcggcta ttgtggccga
     1801 taatacgctt aaagttgttg atcaaaacga tgcggatgct tttatttcgg gcaaagttat
     1861 atcgtacaaa aagtccagcg agacgtataa tattcaggat caggttcagg aatatcgcgt
     1921 agatgttgct gtaaatgtca gttttgtaaa gcctaacggg gatgtgatct gggaagaacc
     1981 gcggatttca tcattcggag tctacgaggc cgagaccgag accgaggacg agggaaaaac
     2041 cgaagctttg gaaaaactgg ccgaaataat agtaaacagg acagttcgaa actggtaaat
     2101 acacttttta caaatagaat ttagcagagg aatatcatga agaataagtt tgcactttcg
     2161 ctcctggcgg cactttcgct tatgattttt acaaacgcaa acctttcggc gataaactca
     2221 aaagccggaa ccgcagccta ttcattcctg aaaatcggag tgggggcgcg ggcggtttcc
     2281 ctgggtaact cgtttgtggg cctggctgat gacgaatcgg caatttacta taatccggcc
     2341 ggcatgagcc gtattcccgg ttatgccctc tcgaccagtt atcacaatta tgtggccgat
     2401 attcagggcg gatatctcag cgccgtactg ccatggggcg acggccgcaa actgggtttt
     2461 gcaatcaatt atcttagctt tggcgacacc ccggaaacca acaatgccgg tgaggtaatc
     2521 ggtaatttcg gcgggggcga cctggcactc accgccgcct actcgcagtc attgatggag
     2581 cagctttatc tggggctggc gggaaagttc atctacgaaa gcatcgattc ttttcattcc
     2641 accggcatgg ctctggatat cgggctgctt tatgagtctc cggacggata caccagcctt
     2701 ggagctgtgg cctcaaatct gggatttcag ctttccgggt tgtcggaaga gcacaaggat
     2761 ccactgccga tcatgttcaa gctcggagtt gcacatcaaa tgcatggggc tccccttaac
     2821 atcgtggccg acgtcggaaa attctcggat aacgatttct ttataggcgg cggcgtggaa
     2881 tacgtgggaa tcgaggccat ccggctgcgt ttgggctaca atacggtcgg ctctgactac
     2941 aagaccggcg gagatagtga aagcctggcc gggctgtcat tcggcctggg tatcaatgtc
     3001 agtaaactga cattcgacta cgcctatgtg ccgtttgcag atctgggcaa ttcgcacagg
     3061 atatatattg caagaaggtg gtagaaaaat ctgaggggga gtatgaaaag agcactttta
     3121 ataattgcgg cggcaattct cgcattagca attgcagcct gcgaaaaaga acctgaggcc
     3181 agggcgcgat ccacggttct ggattttgtt aaggaactgc gttcagaggg cactattgaa
     3241 ctggacaact atctcatact gaatgagctg gtgcgtgaaa atgccgagaa tgtttatact
     3301 tataatgatt ccctgtcagt ttccgagaac atcgagcagt tcagagagtt gttcgagccg
     3361 aacggcaggg tgcgccgtct ctggacccag aaacagataa tcgtgggaga atcagaggtt
     3421 ctgggcgaca ccgcttatgt cgaggtctcc ttcattgacc gggaagaaag aagacagttt
     3481 tataataaga tgggtttgaa gaggaccgat gagggctgga aaatctttgc atttaaactt
     3541 ctttgagggg taaagaagtt aaccgaaaat ttcggtctct gggattctat tgctgatatc
     3601 gtaatttcac tgtttttaca ggttttctca gaatccagcg gtcaaataga gacacatctg
     3661 ctgaaagtca gcgccggaat taacaatatt gaggtaactg ccacggaaac cagggcagaa
     3721 aaatggtctc ctgagattag tcccggaata atcttatttt taaaaaaccg tttgcattat
     3781 atcaagatac acttaagttt ttctggtcat gggggcgtag attatggagt tttgagcatt
     3841 tatggaattc ggaacggtaa caaagctcga aaagggccac ggatacggct tcatacagac
     3901 cgaagataaa aaagaggtat tttttcacca gcgctggcta aggtacatca aattccgtga
     3961 cataaatatc ggttccaaag tggtctttga tctccagaaa ggacatcgcg gccttaaagc
     4021 attaaatatg cgtttcgcac ccgacgacga gaatttcctg aagaatttca ccccgcgaaa
     4081 tagaaaataa aatcggcttt tcggaattaa tatccggctt tgctgatccg ccaaaaatca
     4141 ccattttttg ctggtaaaag cagatgcatt ggctttatat agagatatga tgcagttttg
     4201 ttttatcgac accgagacaa ccgggctttt tacctcatac ggggacagga tttgcgagat
     4261 cgggcttctg gcggtcgacg agactctgga gataatcgag agctttgaaa gcctggtaaa
     4321 tcctc
//
LOCUS       DSEB01000314            3544 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002839, whole genome shotgun sequence.
ACCESSION   DSEB01000314 DSEB01000000
VERSION     DSEB01000314.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002839"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            40..1866
                     /locus_tag="ENO07_05710"
     CDS             40..1866
                     /locus_tag="ENO07_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01499.1"
                     /translation="MNSKVRISGLRIEFETISTGGERVSTPDNPDPDRPGYPSFRGFD
                     HLQSYYSEIEAKPSENVRGVLTLNYLGHVPENPIDEIFYENRGRVKTVLTDEGTMELN
                     DIERLKVYNASISWEDQWFNAEGFYRSGHYHWGYEGDFFGLYREANYGPNIDIYNADA
                     PLGFEFAGKKAIDGLKSAIGPQLWWGANPAVLMKYRRTIGSVTATGIYQEDLEDRTDA
                     VSSVAIPLPKTRKATIHLETQTGPFKIEIGGIWSGENKSGQTYQLVEGESGDYRLFQA
                     RIQSSDAFGAKFKLSYSKGRLNWYLQGASMGLVADGGPTATQTFTGWWLKDSGKGNQR
                     NILTGLSVRFGNLEVAPNFLWQKPLEGPIPGDVPEPGRPRNVLDDPFAVRENREATAF
                     EFLLTYDPTPATWMYTWDSDMREDARLAATFGFIFRHFPTTQDASIGFLADGRTTFAF
                     DGATPPRDLWEAYARIVSKARPDLGLIANLYAGEGEPNGNDDRLIKRYGADIRLVYGS
                     AKFITSVKLNDWGPYDYHRDFNLTYPLQLSADVSKILGKPEWFDLPQTRLGISATYRT
                     LDQYSPRYCPIKIDGECVPDAPGFDNGSEWEIRTYLHMNIGM"
     gene            1944..>3544
                     /locus_tag="ENO07_05715"
     CDS             1944..>3544
                     /locus_tag="ENO07_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008843718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDS01500.1"
                     /translation="MTVAEKIGQLSLVNSIYGKITKPLRQTIAEGKIGGILNEVDVNV
                     VNELQQTAVEESRLGIPLLVGRDVIHGFKTIFPIPLGLASTWNPEIPERCGRISASEA
                     SATGINWTFAPMIDISRDPRWGRIAESFGEDPYLTGVLAKAIVYGFQGDDLSSPGCIA
                     ACAKHFAGYGACESGKDYNTANIPENELRNVYLPPFKAAFDAGAASVMTSFSDLDGIP
                     ATANEFLLKQILRQEWQFDGFVVSDWEAISQLSVHGLTASDKDSACEAANAGVDMEMV
                     SRTYADHLADLLIEGKISQDRIDTMVSNILRAKFKLGLFDRPYTETSSVNTDKSHEYL
                     EAARCAAVQSCVLLENKHNILPLSKDKLSSIAVIGPLADDRYEQLGTWIFDGDIELSQ
                     TPLQAIKAFMKKSAEVNYCRAMATTRSRSREDFGEAVETVRKSDVVILFLGEESILSG
                     EAHSRADISLPGIQNELIEEIAALGKPIVLVIMAGRPLLLEKARKNVDAILYAWHPGT
                     MAGPAIAELLFGIASPSGKLPVTFP"
BASE COUNT          920 a          811 c          962 g          851 t
ORIGIN      
        1 ctggaagctc gaggtgacaa ggcggctcgc ttatctgaga tgaacagcaa agtcaggatc
       61 agcggcctcc gaatagaatt tgaaaccatc agtaccggtg gcgagcgggt cagtacaccc
      121 gacaaccccg atccggacag accggggtat ccttcgtttc gtggatttga tcatctgcag
      181 tcttactatt ctgagataga agccaaaccg tcggaaaatg ttagaggtgt gctaactctg
      241 aattatctcg gccatgttcc tgaaaatccc atagatgaaa ttttctacga gaatcgcggc
      301 cgggtcaaga cagttttaac tgatgaaggg actatggaac taaacgatat tgagcgctta
      361 aaagtttaca acgcttcaat atcgtgggaa gatcagtggt ttaatgccga aggattttac
      421 cggagcggac attatcactg gggttatgaa ggtgacttct tcggtcttta cagggaagcc
      481 aattatggtc cgaatataga tatttataac gccgatgctc cgcttggatt tgagtttgca
      541 ggcaagaagg ctattgacgg gctaaaatcg gctattggtc cgcaattgtg gtggggcgcc
      601 aatccggccg ttttgatgaa atatcggcga accatcggct ctgttacagc aaccgggata
      661 tatcaggaag atctcgagga ccgaacggat gccgtcagtt cggttgcgat cccgctcccg
      721 aaaacaagaa aagcgacaat tcatctggag acccaaacgg gaccgtttaa aatagaaatt
      781 ggcggaatct ggtcggggga gaacaagtcc ggccagactt atcagctcgt tgagggcgag
      841 tctggagatt atcgcttatt tcaggcccgg attcaatcct cggacgcctt tggcgcaaaa
      901 tttaagcttt catattcgaa ggggaggctc aactggtatc tgcagggtgc gtccatggga
      961 ttggtcgctg acgggggtcc gacggccact cagacattta cgggttggtg gctgaaggac
     1021 agcggcaagg gcaaccagag gaatatcctg accggtctct cggtcagatt tggcaatctt
     1081 gaagttgctc caaacttcct ctggcagaaa ccgctcgagg gtccaatacc gggcgacgtt
     1141 ccggaaccgg gacggccccg aaatgtgctg gatgacccgt tcgcggtccg cgaaaatcgt
     1201 gaggccacag cattcgagtt tcttctgaca tatgatccga ccccggcgac ctggatgtac
     1261 acctgggata gcgatatgcg ggaagatgca agactcgcgg cgactttcgg tttcatattc
     1321 agacatttcc cgacaacaca ggatgcatca atcggatttt tggctgacgg cagaacgacg
     1381 ttcgcatttg acggtgcaac tccgccccga gatctgtggg aagcatatgc tcgaattgtg
     1441 tccaaagccc gcccggattt agggctgata gctaatttgt atgccggtga gggagaacct
     1501 aacggcaatg atgaccgcct gattaagcgc tatggcgccg atataaggct cgtttacggt
     1561 tcggcaaaat ttattacatc ggtgaaactg aatgattggg gtccctatga ttatcacagg
     1621 gatttcaatc tgacttatcc tctgcagtta tccgcggatg tatcgaagat attgggtaaa
     1681 ccggaatggt ttgaccttcc tcagacacgg cttggcatta gcgcgaccta tagaacgctg
     1741 gatcagtatt cgcctcgcta ttgcccgatc aagattgacg gtgaatgtgt ccccgacgcg
     1801 ccggggtttg ataacggtag tgagtgggaa atcaggactt atctgcatat gaatatagga
     1861 atgtaagcgg aatggagatt cttggaaaaa caacaaagca ttcgagagct atagaaaagc
     1921 ggattgaaga gctgatacaa gaaatgaccg ttgctgaaaa aatcggtcag ttgtcattag
     1981 tgaatagtat atatggcaag attaccaaac cgctcaggca gacgatcgcc gaaggtaaaa
     2041 tcggcggtat tcttaatgaa gttgacgtca atgttgtaaa cgagcttcag caaacagcgg
     2101 tagaagagag ccgcctgggc attcccctgc tggtcgggcg agatgtcata cacggattta
     2161 agacaatttt ccctatcccg ctgggcctgg cctcgacctg gaatccagag attcctgaac
     2221 gatgtggacg tatttcagca agcgaagcgt cggcgaccgg tatcaactgg acctttgctc
     2281 caatgattga catcagtcgt gaccccaggt ggggaagaat cgctgaatct tttggtgaag
     2341 atccctatct cactggagtt ttggccaagg caatagttta cggttttcaa ggtgatgatc
     2401 tcagcagtcc gggttgcatt gccgcctgtg ccaagcattt tgccggctac ggcgcctgcg
     2461 aaagcggcaa agactataat accgccaata tccctgaaaa cgaactcagg aatgtatatc
     2521 ttcccccatt taaggcagct tttgatgccg gggcggcatc ggtgatgaca tcattcagtg
     2581 atctcgacgg cattccggcc acagccaacg agtttctctt aaagcagatt ctccgtcagg
     2641 agtggcagtt tgacggattc gtggtcagcg attgggaagc aatcagccag ttgtcggtgc
     2701 atggtttgac ggcaagtgac aaagattctg cctgcgaagc agccaatgcc ggggtcgata
     2761 tggaaatggt cagtcgaaca tatgccgatc atcttgccga tctgctgatt gagggcaaga
     2821 tttctcagga tcgtatcgac actatggtct caaacatact ccgggcaaaa ttcaaactgg
     2881 ggctgtttga cagaccctat accgaaacaa gcagtgtgaa taccgacaaa tctcatgagt
     2941 atcttgaggc cgccaggtgt gcagccgtgc aaagttgtgt tttactggaa aataaacata
     3001 acattcttcc cctgtcgaaa gacaagcttt catcgattgc ggtaatcggt cctctggctg
     3061 atgatcgtta tgagcaactt ggtacctgga tttttgacgg cgatatcgaa ttgagtcaaa
     3121 cccccctgca agctatcaaa gcattcatga aaaaatccgc ggaagtcaat tattgccggg
     3181 cgatggcaac aacccgaagc agaagccggg aggattttgg cgaagccgtt gagacagtca
     3241 ggaaatcgga tgtggttatt ctatttctcg gggaagaatc aattttatcc ggtgaggcac
     3301 actcccgggc cgatataagt ctgccgggca ttcaaaatga actgatagag gaaattgccg
     3361 cactgggcaa gccgatagtc ctggtaatca tggccggaag gccgctgctg cttgaaaaag
     3421 cgagaaaaaa tgtcgatgcc attttgtatg cctggcatcc cggaactatg gcgggcccgg
     3481 ctattgccga gctcctgttt ggtattgcgt cgccttccgg aaagctgccg gtcacatttc
     3541 ccag
//
LOCUS       DSEB01000315            3546 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002838, whole genome shotgun sequence.
ACCESSION   DSEB01000315 DSEB01000000
VERSION     DSEB01000315.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002838"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            120..845
                     /locus_tag="ENO07_05720"
     CDS             120..845
                     /locus_tag="ENO07_05720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01501.1"
                     /translation="MTKKVLTWPAVASIIFLFFLLTGCGDDDSINPESLTITDIDGNI
                     YSVVKIGDQLWMADNLKVTHYRNGDPIANVTVVAEWSSMTAGAYCDYNNEVTNAAVYG
                     RLYNWYAVNDSRGIAPEGWHVPSDSEWKQLERHLGLSNEEADSAGWRADGLGGKLKAT
                     YNWQLPNDGATNETGFTALPGGNRYFGSGNTFNSINQAAFFWTAGESEGRTWHRALLY
                     DRSGIFRYYEPKNVGLSVRCVKD"
     gene            complement(1008..>3546)
                     /gene="uvrA"
                     /locus_tag="ENO07_05725"
     CDS             complement(1008..>3546)
                     /gene="uvrA"
                     /locus_tag="ENO07_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDS01502.1"
                     /translation="VGTVTEIYDYLRLLFARIGVPHCFNCGKKIAQQTSSQMVDQIME
                     LKEGTRLQILAPLVRGRKGEHKEIVEKAGKEGFVRIRIDGEVYPIDEAPDMEKNKKHD
                     VEIVIDRLVISEKVRRRLADSVEIALKIGGGMAMVDIIGGEEMLFSENYACVDCGISY
                     EELTPRMFSFNSPYGACPACDGLGHRMEIDESLVIPNPDLPINEGAIAPWGADLANWY
                     RYQLRGLANHYGFKLSSHFSKLPRKVQNVILHGSGNTEIKFAYEHASGRGNARGEFVS
                     SFEGVIPHLERRYRQTESSGVRNWIEQYMSITECPLCGGARLRRESLAVYIADKNIHD
                     ITRMSIKAAREFFEMIKLSKRQQKIAHQVMKEIRERLGFLVNVGLEYLTLDRAASTLS
                     GGEAQRIRLATQIGSRLTGVLYILDEPSIGLHQRDNNRLLSTLTELRDLGNTVVVVEH
                     DRDTILAADYVVDLGPGAGKHGGEIVAAGTPAQIKRQTKKSLTGKYLAGKSKIPVPKH
                     RNGGNGDMLNLKGAAGNNLKEINIDIPLGKFICVTGVSGSGKSTLIEETLYPILSSRF
                     YNSRRKPLPYKKILGLEHIDKVIDIDQSPIGRTPRSNPATYTGTFTFIRDLFSQTPEA
                     KLRGYKPGRFSFNVKGGRCEACEGDGIIKIEMHFLPDVYIPCEVCKGRRYNNETLEIR
                     YKGKNIAEVLDMTVAEALEFFGNIPPLKRKLMTLYNVGLGYIHLGQQATTLSGGEAQR
                     VKLSTELSKTATGRTFYILDEPTTGLHFEDIKMLLNVLNELVNRGNTVLVIEHNMDVI
                     KTADWIIDLGPEGGDDGGYVIATGTPEQVAKNGKSYTGQFLKQELGD"
BASE COUNT          800 a          961 c          888 g          897 t
ORIGIN      
        1 ctcatgcgcg gcgcatcggc ctcggtgcgc cgcaaccctg gttcaaagct gaacgcattg
       61 aactcacaat tcacgccgat tcttcaattt tatgggattt ttttaggagg agttatttta
      121 tgacgaaaaa agtcctcacc tggcccgcgg tcgcttcgat cattttcttg ttctttttgc
      181 tgacaggatg cggtgatgat gatagtatca atcctgagag cctcacaatc accgacattg
      241 atggtaatat atatagcgta gtcaaaatcg gtgaccaatt gtggatggcc gataatttaa
      301 aagtgacaca ttatcgcaat ggcgatccta tcgccaatgt caccgttgtt gctgaatggt
      361 cctcgatgac tgccggggca tattgtgatt acaataatga agtcaccaat gccgcagtat
      421 atggcaggct ctacaattgg tatgccgtga atgacagcag agggattgct ccggagggat
      481 ggcatgtgcc ctcggattct gaatggaagc agcttgagag gcatctgggc ctgagcaatg
      541 aggaagccga ttccgctgga tggcgggctg acggattggg cggcaagctc aaagcgactt
      601 ataactggca gcttcccaac gatggcgcca ccaacgagac cggtttcaca gccctcccgg
      661 gaggcaaccg ttattttggc agcggcaata catttaactc aataaaccag gcagcgtttt
      721 tctggaccgc cggggaatcc gagggcagga catggcaccg tgccctctta tatgaccggt
      781 caggcatctt ccgctactac gaacccaaaa atgtcggcct ctcggtccgc tgtgtgaagg
      841 attgatggta accttgcctg gggccacatc cggctcaagg ctggaagaga catcgcgcga
      901 cagattgatg tgtcgtgatg aataggtaag gggttgactg ttgtcgtcgt gcgaggcctt
      961 ggtcccgatt tatcgggatt gtgatctcgc actcaaatga tcctttatta atcccctaat
     1021 tcctgcttca aaaactggcc ggtgtatgat ttgccgtttt tggctacctg ttcgggggtg
     1081 ccggtggcga tgacgtaacc gccgtcatct ccgccctccg ggccgagatc aatgatccag
     1141 tcggcggtct tgatcacatc catattatgc tcgatcacaa gcaccgtatt gccgcggttg
     1201 accagctcat ttaagacatt cagaagcatc ttgatatcct cgaaatgcag tccggtggtg
     1261 ggctcatcga gaatatagaa tgtcctcccg gtggcagtct tggaaagctc ggtggaaagc
     1321 ttgaccctct gcgcctcgcc gcccgataat gtggtggcct gctggccgag atgaatatag
     1381 cccaggccga cattatacag tgtcatcagc ttgcgcttga gcgggggaat atttccgaaa
     1441 aattccagtg cctcggccac agtcatatcc agcacctcgg caatattctt gcccttgtac
     1501 ctgatctcca gcgtctcatt attatagcgc cggcccttgc agacctcgca ggggatataa
     1561 acatcgggca ggaaatgcat ctcgattttt attatcccgt ccccctcgca ggcctcgcat
     1621 cgcccgccct tgacattgaa cgagaaccgt cccggcttgt aaccgcgcag cttggcttcg
     1681 ggagtctggg agaaaaggtc gcggataaat gtgaatgtcc cggtatatgt agcgggattc
     1741 gaacgcgggg ttcttccaat cggcgactgg tcgatatcga tcaccttatc aatatgctcc
     1801 aggccgagaa ttttcttata gggcaacggt ttccgtcgtg aattgtagaa tctgctgctc
     1861 agaatcggat acaatgtctc ctcgatcaat gtcgatttgc ccgatccgga gactccggtc
     1921 acgcagataa acttgcctaa cgggatatcg atattgatct ctttcaggtt atttccggcc
     1981 gcacccttga gattgagcat atccccgttg ccgccgttac gatgtttcgg cacagggatc
     2041 ttcgattttc cagccagata ttttccggtc agcgattttt tggtctggcg cttgatttgt
     2101 gcgggagtgc ctgccgccac aatctcgccg ccatgtttac ctgcgccggg tcccagatcc
     2161 accacataat cggccgcaag tatcgtatcg cgatcatgct caaccaccac aacagtattg
     2221 cccagatcgc gaagttctgt caaagtgctc aagagacggt tattatcccg ctgatgaagg
     2281 cctattgagg gttcatcaag tatatataaa acccctgtca ggcgtgaccc tatttgggtt
     2341 gccagccgta tcctttgagc ctccccgccg gagagggtgg aagccgcgcg gtcaagggtc
     2401 aaatactcca gccccacatt gaccagaaac cccagccgtt cccggatttc cttcataacc
     2461 tgatgtgcta tcttctgttg gcgtttgctg agtttgatca tttcgaagaa ctcacgggcc
     2521 gccttgatcg acattctggt aatatcatga atatttttat cagcgatata gaccgccagc
     2581 gactcccgcc tcaatctcgc gcccccgcat aacgggcatt ctgtaatcga catatactgc
     2641 tcgatccagt tgcggactcc tgaagattcg gtttggcggt atcttctttc cagatgtggg
     2701 attacaccct caaatgagga cacaaattca cctcgggcat tgccgcgtcc ggaagcatgt
     2761 tcataggcga attttatctc agtattaccg gagccatgca aaatgacgtt ctgaaccttt
     2821 cttggcaatt tgctgaagtg actggagagt ttaaaaccgt aatgattcgc aagtccgcgc
     2881 aactggtacc gataccagtt ggccagatcg gcaccccatg gcgcaattgc accctcgttt
     2941 atcggcaggt caggattggg aatcacgagg ctctcgtcaa tttccatccg atggcccagg
     3001 ccgtcacagg ccgggcaggc cccataaggc gaattgaacg aaaacatgcg cggagtcagc
     3061 tcttcatacg atataccaca gtcaacacag gcgtaattct cggagaagag catctcctct
     3121 ccgccgataa tatcgaccat agccataccg ccgcctatct tcagggcgat ttcgaccgaa
     3181 tcggccaggc gccggcggac tttttccgaa atcaccagcc gatcaatcac tatttcgacg
     3241 tcatgtttct tatttttctc catatccggg gcctcgtcga tcggatagac ctcgccgtcg
     3301 atgcgaatgc gcacgaaacc ctcttttccc gctttttcga cgatttcctt atgttcgccc
     3361 ttgcgtcccc gcaccagagg cgccagaatc tgcagcctgg tgccctcttt cagctccatg
     3421 atctggtcca ccatctgcga ggaggtctgc tgggcgatct ttttaccgca gttgaaacag
     3481 tgcggcacgc cgatgcgcgc gaacagaagt ctaagataat catagatttc cgtcacagta
     3541 cccaca
//
LOCUS       DSEB01000316            6583 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000718, whole genome shotgun sequence.
ACCESSION   DSEB01000316 DSEB01000000
VERSION     DSEB01000316.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6583)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6583)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6583
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000718"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            812..2161
                     /locus_tag="ENO07_05730"
     CDS             812..2161
                     /locus_tag="ENO07_05730"
                     /inference="COORDINATES: protein motif:HMM:PF04055.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HDS01503.1"
                     /translation="MYKISKFNHIRPWGEGHYIAYNARSGALGLMDEKNYQTCQNVFG
                     KIRNGAESEFTPEEAHLVRQLEYGRFIHHDYYDELEELKFQHHQARFDQSSLGLSIAP
                     TLACNMDCGYCYEGNKKGRMQPEIVETILDFIETRAAGIRSLDINWYGGEPLLAMEII
                     EDLTESMLDMAEEYKFAFNASMITNGYLLTNENTDRLLDNLKVSNIQITLDGPSDIHN
                     KKRPLKSGKDSFDTILKNIQYASRKMQVNIRVNIDKSFTSETIQRLLNELDEAGLKNN
                     VGLYFGRIEAATSACSNISESCYGAEDFSKVETDFYRLLLENGFSITSLPQPMGTVCF
                     SQLISSHLLDPEGYIYKCYNYVGDIPRAMGNIKENINYNHPNFTRLFNFDAFEHEECR
                     NCSILPICMGGCPARRADGDLKSGEACETWKHNLGPMLEIIARSRQSQAQKAAKETT"
     gene            2158..3291
                     /locus_tag="ENO07_05735"
     CDS             2158..3291
                     /locus_tag="ENO07_05735"
                     /inference="COORDINATES: protein motif:HMM:PF04055.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDS01504.1"
                     /translation="MSQEVQERKYYPHAVVWEITFACNMRCLHCGTAAGQKRSDELTH
                     EEAIALVDELCGLGAQEITLSGGEPLLRKDWPQLAQRIIDGGSKAYLITNGYAVDEKI
                     VDEFERINFTNIGVSFDGTEPTHNHIRQREKSFKRAKNAMQMMSDRGNIRFCAITQVS
                     NMNLNEMDEIKQILQDVGCRQWRIQMTTSTGRMKEQDNMVLTTDKFPYLVDKLLEYKE
                     EGKIDIDVGENIGFYGCKGTQLWDGKPYFGCFAGMRVAGITSNGEVRGCLSMPEYFNE
                     GNIRDSSFTEIWDDPNNFMYNRVFRSSDAGGWCKECKYLPLCRGGCATTSFSATNMRA
                     NNPYCMYQIEKANGIECEDNEMIQSLLDHVHKQLGGKVETVEK"
     gene            3245..4714
                     /locus_tag="ENO07_05740"
     CDS             3245..4714
                     /locus_tag="ENO07_05740"
                     /inference="COORDINATES: protein
                     motif:HMM:PF02310.17,HMM:PF04055.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HDS01505.1"
                     /translation="MYTNSWAGRSRRLRSSGAPSILLAYPSAFYAAGWGTRLELKSSQ
                     LLLASYLAQYFSVEFADFEISIGAPNSASQVRRFERKVKKYLAESNFDILALSCWASL
                     SYTAALRVARICRELYPDKLIVVGGYHPTACPHDFLLPENLFDYVIIGEGENALREIA
                     EGFSTTGRPSQTQIVKSDVFKNEQFVGFNWDIVENFTNAHYPDGLPNIYLYLSRGCPF
                     GCSFCMEPLKDRSWRAFSPERSVEEMFNAAERFNSIGVAICDACFGKSARWRKEFFRR
                     LVDADPDFWIVVETRHEYLDQEDIELLSNIKAEIQFGLESASPEMLLLMKKCKNPDKY
                     LEKFSRVSNMLSDNGILHRANLIFNHPGETQKSLEETFRFIDNELTKTGRYLIWASAE
                     YMHFPGCELYTNMDFFKKRFGSKFKGGEWWKKEEDQFGASIANEPSADLAGERAKLWN
                     KMFIQRDEAMKAALAPQAFKFAAAKYFPDWKNDPRFRQN"
     gene            4689..6449
                     /locus_tag="ENO07_05745"
     CDS             4689..6449
                     /locus_tag="ENO07_05745"
                     /inference="COORDINATES: protein motif:HMM:PF00664.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDS01506.1"
                     /translation="MTQDSGKIRRLLAYLKPYWLLEIVTFIVMAAIAVLGIAVPAALQ
                     YMIDTLIPSIASQAGEAVKIRPVIIFGLVLVAIYVGQFLLAWLRDYLAAYIGANIIMN
                     MRSDLFIHVERLSLSFFQRSQVGEIMSRVLSDVNRIQGLLTTTLLMFFTNVFMLIAII
                     IYLLNLNWYLTLVALIPVPFTILLTHKFGKRLHQIVRMLQEKLAHLSARLQETLVAIK
                     TVKAFGQEKREKKHVDHVMKDLTGLYIKNSVVTSVTTQIINFVSMLGPIIILAWGTYL
                     IAGGSMQIGELMAFYILLTYLYSPVEGLARINIEVQSAMASVNRVYEYLDIPPAVEEA
                     PEPVEIEKARGEIEFKDVSFAYETGGFKFEHLNLKIAPGEKVAIVGPSGSGKSTIINL
                     LMRFYDPLSGEITVDGIDLRKMSFKSLRDNIVLVDQEPLLFRSTIYENIVYGIPDVDE
                     KDVIEAAKNANIHAYIEALPEKYESQVGERGVTLSGGEKQRICLARAIIRDPSILILD
                     EATSALDSVSEQLIQDSLSRVLRDKTAIMIAHRLSTIQHADRIVVLQNGRIIDQGTSK
                     EIMQSCELYRELVEKQQILP"
BASE COUNT         1775 a         1578 c         1639 g         1591 t
ORIGIN      
        1 atataaggta ttctatgtat caagcatcgg gctttgacag cgttgccttc agactgcatg
       61 atgatacttc agcgcacact acttatatga tgtgcaaaat aggcgatttg tcactaaatg
      121 tggcaaatcg tttgcagtta ggtaaaggga tctaaacctt aaagaggagg tgtgaatgga
      181 tttcatcatt gatcccatgg atattacaca gggatggtgc acacttaagt gcacaactct
      241 ttgtaagaac attgcttacc cgatgtacgg ggtgcctatt tagagagatt gaaaccaggg
      301 ccaatattgc cctggttttg cactgaaagg cataagccgc ttcgatttca gctattgata
      361 tcggagcatt ttgatgtgcc gcagttaagg aaaaccgagc ctaacctatc atgggaaaaa
      421 tgagaggagg aagcatgaac tatcttattg atcctatgga aatcaccgcg ggctggtgtc
      481 cgctcaaatg ctcgaccttg tgcaagagca tcgtatatcc gatgtatggc gttcccccgg
      541 gaccgataga gatgtaatac tcttttcgta acggtggaca aaggcgtcct tccgacgtgg
      601 aactatccgg ctattcgaag gcatcgctga agaatagctt aatttcgggg gacgctctca
      661 aggctttttc cgattttgat aatccttttg gcggattttg acgttcgaag cggaaatccc
      721 tttaactgca aaatccaatg gtctccgact gcggcacatc ataaatttac aggagtcgga
      781 tccaacatta aatactattc gggattctaa catgtacaaa atatcaaagt ttaatcatat
      841 caggccctgg ggagaaggac attatattgc ctataatgct cgcagcggcg ctctgggctt
      901 gatggatgaa aagaactacc aaacctgtca gaatgttttt ggaaaaattc ggaacggcgc
      961 tgagtccgag ttcacgccgg aagaagcgca tctggtaaga cagcttgaat acggcagatt
     1021 catacatcat gattactacg atgaattgga agagctgaaa ttccagcatc accaggccag
     1081 atttgaccag agctcactgg gcctgtccat agcccccacc ctggcctgca acatggactg
     1141 cggctattgc tatgaaggca ataaaaaggg acgaatgcag cccgagattg tggagacaat
     1201 cctggacttc atcgaaacac gcgctgcggg catacgcagc ctggacataa actggtatgg
     1261 cggcgaaccg cttctggcca tggaaatcat cgaggacctg accgaatcga tgctcgatat
     1321 ggcagaagaa tacaagtttg cattcaatgc ctcgatgatt accaacggct acctgctgac
     1381 aaacgagaac accgaccgtc ttttagataa cctcaaagta agcaacatac agatcactct
     1441 tgacggtccc tcggacatac ataacaaaaa aaggccgctc aaaagcggca aggacagttt
     1501 cgacacaatt ctcaaaaaca ttcaatatgc ctcccggaag atgcaggtca atattcgggt
     1561 caatatcgat aagagcttca catctgaaac tatccagaga ctgctgaatg aacttgatga
     1621 ggccgggctc aaaaataatg tcggactcta ttttgggcgt atcgaggcgg ccacctcggc
     1681 ctgctccaac atttccgaga gctgttacgg cgcagaggat ttttccaaag tcgaaaccga
     1741 tttctaccgc ctgcttctgg aaaacggatt ttcgattacc agccttcccc agccgatggg
     1801 tacggtttgt ttcagccagc tgatcagttc acaccttctg gatccggaag gatatatata
     1861 taaatgctat aactacgtcg gtgatattcc cagagcaatg ggcaatatca aagagaatat
     1921 aaattacaac catcccaact tcacacgtct tttcaacttt gacgccttcg agcacgagga
     1981 atgcagaaat tgcagtattc ttccgatctg catgggcgga tgtccggcgc gccgtgccga
     2041 cggcgacctg aaaagcggtg aggcatgcga aacctggaaa cataaccttg gacctatgct
     2101 cgaaattatt gcacgttcgc ggcagagtca ggcccaaaaa gccgcaaagg agacgacatg
     2161 agccaagaag tacaagaacg gaaatattat ccccatgctg tggtatggga aattacattc
     2221 gcctgcaata tgcgctgcct gcattgcggg actgcagccg gccaaaagag gtccgatgag
     2281 ctgacccatg aggaggcaat tgctctggtg gacgaactct gcggcctggg cgcgcaggag
     2341 atcacgcttt ccggcggtga gccgcttctc agaaaagact ggccccagtt ggcccagagg
     2401 attatcgatg gcggttccaa ggcatacctg attaccaatg gttatgctgt cgacgaaaaa
     2461 atcgtcgatg agttcgaaag aattaacttt accaatatag gcgttagctt tgatggcacc
     2521 gaacccactc ataaccatat tcgtcaacgt gaaaagagct ttaaaagggc caagaatgct
     2581 atgcagatga tgtccgacag gggcaacatt aggttctgcg caatcacaca ggtctcgaat
     2641 atgaatttga acgaaatgga tgagatcaag cagattctgc aggatgtggg atgccgtcag
     2701 tggcggatcc agatgaccac ctccactgga cgtatgaaag aacaggataa tatggttctg
     2761 accacggaca agtttcccta cctggtggat aagctcctgg aatacaagga ggaaggcaag
     2821 atcgatatcg atgtgggcga gaatatcggc ttctacggct gcaagggaac tcagctctgg
     2881 gacggcaagc catatttcgg atgtttcgcg ggcatgcgtg tggccgggat aacatcaaat
     2941 ggcgaggtgc gcggatgcct ttccatgccg gagtacttca atgaaggcaa tatcagggat
     3001 tcatccttca ccgagatctg ggatgaccca aataatttca tgtacaaccg cgtgttcagg
     3061 agctctgatg ccggcggatg gtgcaaggaa tgcaagtatc ttccgctttg ccgcggcgga
     3121 tgcgccacga cctcattttc cgccacaaat atgcgcgcca ataatcctta ctgcatgtac
     3181 cagatcgaga aggccaacgg tatcgaatgc gaagataacg agatgatcca gagccttctg
     3241 gatcatgtac acaaacagct gggcgggaag gtcgagacgg ttgagaagta gcggagcgcc
     3301 gtcaattctt ctggcatatc catcagcatt ttacgccgcc ggctggggta cgagattaga
     3361 attaaagagc tctcaattgc tgctggcttc ctatctggcg cagtattttt ccgtggagtt
     3421 tgccgatttc gaaatatcga tcggcgcccc caattctgcc tcacaggtca ggaggtttga
     3481 acgcaaggtc aaaaaatatc tggctgagag caattttgat atcctggccc tgtcctgctg
     3541 ggcgagcctt tcgtatactg cagcgctacg agtggcccga atctgccgcg aactttatcc
     3601 cgataaattg atagttgtgg gcggatacca tcccaccgcc tgcccccacg atttcctcct
     3661 gcctgaaaac ctcttcgact atgttattat cggtgaaggt gagaacgcct tacgagaaat
     3721 cgctgagggc ttctccacca caggccggcc atcgcagacg caaattgtaa aatcggacgt
     3781 attcaaaaac gagcagttcg tgggcttcaa ctgggatatt gtcgaaaatt tcacaaatgc
     3841 ccattatccg gatggcctcc ccaatatcta tctgtatctt tcacgcggct gtcccttcgg
     3901 ctgttccttc tgcatggaac cattgaagga tagaagctgg cgggcttttt cgccggaaag
     3961 atcggtggag gagatgttca acgctgcgga aaggttcaat tcgatcgggg tcgcgatctg
     4021 cgatgcctgc tttggcaaaa gcgccagatg gcgcaaggag ttcttccggc ggcttgttga
     4081 tgcagatccc gatttttgga ttgttgtcga gactcgtcat gaatatctcg atcaggagga
     4141 tatcgagctt ctttcgaaca taaaagcgga aattcaattt ggattggaaa gcgcctcgcc
     4201 tgaaatgctg ctcttgatga aaaaatgcaa aaatcctgat aagtatctgg agaaattcag
     4261 ccgggtcagc aatatgctct cggataatgg catactgcac cgtgccaacc tgatcttcaa
     4321 ccatcccggc gaaacacaga aaagccttga ggaaacattc cgttttatag acaatgaact
     4381 tacaaagacc ggcagatacc tgatctgggc ctcggcggaa tatatgcact tccccggatg
     4441 cgaactttat acaaatatgg acttttttaa aaagcgtttc ggcagtaaat tcaagggcgg
     4501 agaatggtgg aaaaaggaag aggatcaatt cggcgcaagc attgcaaatg agccctcggc
     4561 cgatctcgcg ggcgaaagag caaagctctg gaataaaatg tttatccaaa gagatgaggc
     4621 catgaaagcg gccctggctc ctcaagcatt caaatttgcg gcagccaaat atttcccgga
     4681 ctggaaaaat gacccaagat tcaggcaaaa ttagaaggct tctggcatat ctcaagccct
     4741 actggctgct cgagatcgtt acatttatag ttatggctgc aatcgcagta ctgggcattg
     4801 ctgtccccgc cgccctgcag tatatgattg ataccctgat tccctccatt gcgtcgcagg
     4861 cgggtgaggc ggtgaagatt cgtccagtaa ttatattcgg cctggtgctt gtggctattt
     4921 atgtcggaca gttcctgctg gcatggctgc gcgattatct ggccgcttat atcggcgcaa
     4981 acatcataat gaacatgcgc tctgatttgt ttatccatgt cgagcggctg tcgctctcct
     5041 ttttccagag gagccaggtg ggcgagatca tgtcccgcgt gctttccgac gtcaaccgta
     5101 tccaagggct tctgaccaca acacttctga tgttcttcac caatgtcttt atgctgattg
     5161 ctattatcat ctaccttttg aatcttaact ggtatttgac acttgtagct ttgattccgg
     5221 tacctttcac tattctgctt acccataagt tcggcaaacg cctgcatcaa atcgtccgca
     5281 tgctccagga aaaacttgcg catctttcag cacgactgca ggaaacactg gtggccatca
     5341 aaacagtcaa ggccttcggg caggagaaac gtgaaaagaa gcatgtcgat catgtcatga
     5401 aggaccttac aggtctctat atcaaaaaca gcgtggtaac ctcggtcacc acccagataa
     5461 tcaacttcgt gagcatgctc ggtccgatca ttattcttgc atggggaacc tacctgatag
     5521 cgggcggctc catgcaaatc ggcgagttga tggcgtttta tatcctgctg acctatctgt
     5581 attcccctgt tgagggtctg gcacggatca atatcgaggt gcagtcggcc atggcctcgg
     5641 tcaacagggt ctatgaatat cttgatatcc ccccggcggt tgaagaagct cccgaacctg
     5701 tcgaaatcga aaaggcccgg ggtgaaatcg agtttaagga tgtttccttt gcatatgaaa
     5761 ccggtggctt caaattcgaa catctgaatc taaagattgc tcccggcgaa aaagtggcca
     5821 tcgtcggccc ctcgggatcg ggcaaaagca cgattataaa cctgctcatg cgcttttatg
     5881 atcctctttc cggcgaaatc acggtagatg gcatcgattt gagaaagatg tcatttaagt
     5941 ccttgcgcga taatatagtt ctggtcgatc aggaacctct tctattccga tcgactatat
     6001 atgagaatat cgtctacggc atacccgatg tggacgagaa agatgtcatt gaagcggcaa
     6061 aaaacgccaa tatacatgcc tatatagagg ctcttccgga gaaatatgaa agccaggtag
     6121 gtgaacgcgg agtaacatta tccggcggcg aaaaacagcg gatatgcctg gcccgcgcaa
     6181 tcatcagaga cccctcgatt ttgattttgg atgaggccac ctcggcgctc gactcagtct
     6241 cggaacaatt gattcaggac tccctttccc gagtcttaag agataagacc gcaatcatga
     6301 tcgcccacag gctttccacc atccagcacg ccgaccgaat cgtggtcctt cagaacggcc
     6361 ggataatcga ccagggcacc agcaaggaga tcatgcaaag ctgcgagctg taccgcgagc
     6421 tggtcgaaaa acagcagata ttgccctgat gcattggata aatcatcctg ggaagttggc
     6481 acctgaatag tgtcatcacg aggaatgaag cggccggttt gcggcctctg agtgacgtgg
     6541 tgatctcctc gatagatata agggaaacac catcccccat ttc
//
LOCUS       DSEB01000317            4676 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001534, whole genome shotgun sequence.
ACCESSION   DSEB01000317 DSEB01000000
VERSION     DSEB01000317.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4676)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001534"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..440)
                     /locus_tag="ENO07_05750"
     CDS             complement(<1..440)
                     /locus_tag="ENO07_05750"
                     /inference="COORDINATES: protein motif:HMM:PF00892.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01507.1"
                     /translation="MKSWLLASLAAAFLFGAYNIFAKMAAGRLTDAMAAFILEFSAAM
                     LVLAYILFGRSIKLDIASVTSKGMLYAVIGGLFIGGGSIFYFYAFRHHAPLAIAGPII
                     FAGATLIMVLSGFIFFKERLDLVTAAGIILTLAGIFLLSISANRG"
     gene            complement(453..2645)
                     /locus_tag="ENO07_05755"
     CDS             complement(453..2645)
                     /locus_tag="ENO07_05755"
                     /inference="COORDINATES: protein motif:HMM:PF01564.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01508.1"
                     /translation="MLTRYDLFILPFLYGFVTLGYQILYVRRLVSIFGSSEIGYTLII
                     TIWLFFTGLGAYMAEKTGIRRGSRYISVAIYGLVAVVFYGAIPRLYDIWNLQPGEVTG
                     LLTMAATSVLALVILCLFSGALFVGIVRTVSAHSRITSLSFVYVVESLGSLAGGVALT
                     FVCLTLIPDRYTALVLFLLIVAFMFVKMDKKLLLMPPRVTVGVMCVFAGVAFALGQFG
                     HQYENQEVVAQTNTAYQHLTLTEYESQYTLYSDGLSFFSYPQVQEAEEIHLTMWQKEE
                     IENIAMIGICPPDKIAEILKYENVRIDIYENDRTLLEFLVKHLPEPAGQYYANPQIKY
                     DWGDPYIDITKNGQDYDFVIINISNPTDAVSNRYFTVDFFEHLSTRLPEDCVLSFSLP
                     GRTHLLGRDLQEFLGTIYNALRTGFNNIKIIPGDDYIFLASNDSSMIDLDVNALLAAK
                     EKNQIQTTSVDSVYLSYRLNEFNLLKAQPIYNNELSAANTVSRPVVYYYNSILWASRF
                     SGLEEAVLKFWKENKYLIYILLFLPLISLLNLVKRSVHRPAYFSICFLAGFSGITMEM
                     SLLILYQSHLGNVYSQIGLLIGLFMVGMALGGALPLRSKGTTIFMKKWFYLGAAIGVV
                     VLFGLAMITRLEGHQFLNYLLILGLSFLSGGFSGYLFNYAAVSYKHKYGAEAPGRFYL
                     ADLLGASLAGVFISVLFIPLIGFGSILALFFVLYLVYVILHRFVIIGV"
     gene            complement(2688..3827)
                     /gene="amrS"
                     /locus_tag="ENO07_05760"
     CDS             complement(2688..3827)
                     /gene="amrS"
                     /locus_tag="ENO07_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HDS01509.1"
                     /translation="MKRRTLIKAMACGAGCACLPFNPVENGLALFQNAYALETTDTLS
                     HVKAMYYKKLEGTAIECELCPRHCLVTDLERGYCGVRENQGGEYYTLVHSRACAMNID
                     PIEKKPLFHFHPGTAAFSIATAGCNVNCKFCQNWDISQVRPEQVTNVELPPDEIVRIC
                     QARSVPTVAYTYSEPIVFYEYMHDTCVEGRKFGIKNVMITGGYIEKEPLKKIIPLMDA
                     IKVDLKSYSQQYYEDVVNGELQPVLDALKIIHDDGMWLELVYLVVPTMNDSTKELTDL
                     CRWVKTNLSDHVPIHFTRFHPTYLMTNLPPTPVKTLEKAYDIARQSGLKFPYIGNVPG
                     HSAEHTYCPGCGELLIERLGFRINKINLKDGMCGKCGTHIPGVWS"
     gene            complement(3831..>4676)
                     /locus_tag="ENO07_05765"
     CDS             complement(3831..>4676)
                     /locus_tag="ENO07_05765"
                     /inference="COORDINATES: protein motif:HMM:PF04015.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDS01510.1"
                     /translation="GLKRDQTNKLISHAMVRLTGSTDSKSAWKKIAAQGDRFGIKPNC
                     LSGRMLSSSPVMADAIVAGLQSAGLRESDIIIWERTNRELERAGYNLNYARNSGLRCF
                     GTDSRGVGYSDSFYQKGKVASLISKIFEQEIDKNINFPILKDHSVAGVSGGLKNLYGL
                     VHNPNKYHPDNCNPYVAELSALPIVREKNVLTILDMTRMQYNGGPGYMPRYLVKPGTV
                     VMSFDPIAIDAYGYKIIEDYRRKNGLPSLKEDKREPLWLETGKRLGLGESDLSQVEII
                     EEDIT"
BASE COUNT         1321 a         1176 c         1054 g         1125 t
ORIGIN      
        1 cccctgttgg ccgaaatcga gagcaggaat atacccgcaa gggtcaaaat tattccggcg
       61 gcggtcacca ggtcgagccg ctctttgaag aatataaatc ccgaaagaac catgatcaat
      121 gtcgcgccgg caaatatgat cggcccggcg attgccagcg gagcatgatg cctgaaggca
      181 tagaaataaa agatggagcc gccgccgatg aagagacctc cgatgacagc atagagcatt
      241 cccttgctgg ttacggaagc tatatccagc tttatcgaac ggccgaataa tatataggcc
      301 agcaccagca tggccgccga aaattccagg ataaaagccg ccatagcatc ggttaacctt
      361 cccgcagcca tcttggcaaa aatattataa gcaccaaaca ggaatgccgc cgccagtgat
      421 gcaagaagcc atgatttcat gataactaac ctctaaactc ctatgataac aaatctatgc
      481 aggatgacat atacaaggta taacacaaaa aacagcgcca gaatcgaacc gaagccgatc
      541 agcggaataa agagcactga tatgaatacc cccgccagtg acgctcccag taaatcggct
      601 aggtaaaagc ggcccggagc ctcggctcca tatttatgtt tatagctgac agctgcataa
      661 ttaaacagat agccggaaaa tccgcctgat aagaacgaca atcccaggat caaaagatag
      721 ttgagaaatt gatggccttc caggcgcgta atcatggcca gaccgaacag tacaactact
      781 cctattgccg ctcccagata aaaccacttc ttcataaata tagtggtgcc cttcgaacgc
      841 agcggcaatg ctccccccag agccatgccc accataaaga ggccgatcag gagaccgatc
      901 tggctgtaaa cattgcccag atgcgactgg tagagaatca agagggacat ctccatagta
      961 ataccggaaa atccggccag aaagcaaatt gaaaaatagg ccggcctgtg aacagatctc
     1021 tttaccagat tcagcagtga aatcagagga aggaacagca gaatatatat cagatactta
     1081 ttctccttcc agaatttcag gacggcttcc tccaaccctg aaaagcggct ggcccagaga
     1141 atggaattgt aatagtagac taccggacgg gataccgtgt tcgcagccga aagttcgtta
     1201 ttatatatag gttgtgcctt cagcagatta aattcgttca gccggtagga caggtagacc
     1261 gaatctacag aagttgtctg gatttgattt ttctctttag cggccagcag agcatttaca
     1321 tcaagatcga tcatagatga atcgttgctg gcaaggaaga tgtaatcatc ccccgggatt
     1381 atctttatat tgttgaaacc ggtgcgcagc gcattgtata tggtacccaa aaactcctgc
     1441 aaatccctgc cgagcaagtg ggtcctgccg ggcagcgaga atgacagaac acaatcctcc
     1501 gggaggcggg ttgaaaggtg ctcgaaaaag tcaactgtaa aatagcggtt gctgaccgcg
     1561 tcggttggat tggatatatt aataatgacg aaatcataat cttggccatt cttggttatg
     1621 tcaatatagg ggtcgcccca gtcatatttg atttgcggat ttgcataata ctgcccggca
     1681 ggttccggca aatgcttgac caaaaattcc agaagagtcc tgtcgttttc atatatatcg
     1741 atccggacat tttcatattt caatatctcg gcgattttat cgggcgggca gataccgatc
     1801 atggctatat tttcaatttc ttccttctgc cacatcgtca gatggatttc ctccgcctct
     1861 tgaacctgcg gatatgagaa gaagcttaag ccgtctgaat acaaggtata ttgagactca
     1921 tattccgtca gagtcagatg ctgatatgct gtattggtct gtgcaaccac ctcctgattt
     1981 tcatattgat ggccaaactg tcccagggca aaagccacgc ccgcgaatac acacatcaca
     2041 ccaacagtca ctcttggcgg catcagtaaa agcttcttat ccattttgac aaacataaat
     2101 gcgacaataa gcagaaatag aaccagggcg gtgtatctgt ccgggataag ggtcaggcag
     2161 acaaaagtta atgctactcc tcccgcaagg gagcccagcg attccactac atatacaaac
     2221 gatagagaag ttatcctgga atgggccgag actgtccgaa caatgccaac aaataaagcc
     2281 cctgaaaaaa gacagagaat taccagtgct aaaacagagg tggcagccat tgtcaacagc
     2341 ccggtaactt cacccggttg aagattccag atatcataca aacgggggat cgccccgtag
     2401 aatacgaccg ccaccaaccc atagatggcg accgagatat agcgcgagcc tcttctgatt
     2461 cccgttttct cggccatata tgcgcccagg ccggtgaaaa aaagccagat ggttattatc
     2521 aatgtatagc cgatttcaga ggagccaaaa attgatacaa ggcggcgcac gtaaaggatt
     2581 tgatacccga gcgtcacaaa tccgtagaga aatggtaaaa taaaaaggtc atagcgtgtc
     2641 agcatgacct aatagtaatc atttaaaatg agatcacaaa acaaaatcta tgaccagacc
     2701 cccgggatat gggtgccgca tttaccgcac attccatctt tgaggttgat cttattaatt
     2761 ctgaatccaa gcctttcaat aagaagttct ccgcatccgg gacagtatgt atgctccgcg
     2821 ctgtgaccgg ggacattacc gatataagga aatttcaggc cggactgccg ggcaatatca
     2881 taggccttct ccagagtctt tacaggagtg ggcggaagat tcgtcataag gtaggtagga
     2941 tgaaatcttg tgaaatgtat cggcacgtga tcggaaaggt tagttttgac ccaccggcac
     3001 agatctgtca gctctttcgt gctgtcattc atagtcggaa cgaccagata gacaagctcc
     3061 aaccacatcc catcgtcatg gatgattttc agagcatcga gcacaggctg aagctccccg
     3121 ttaacaacat cctcataata ttgctgcgaa tatgatttca gatcgacctt gatagcatcc
     3181 ataagaggta taatcttctt cagcggttct ttctcgatgt agccgccggt aatcataaca
     3241 ttcttgattc caaatttccg tccttcaaca caggtatcat gcatatattc ataaaataca
     3301 atcggctccg aatatgtata ggccactgtt ggcactgagc gcgcctggca aatcctgact
     3361 atctcatccg ggggcaattc gacattggta acctgctccg gacgtacctg ggagatatcc
     3421 cagttctggc aaaacttgca attgacattg cagccggcgg tggcgatcga aaaggccgca
     3481 gtgccgggat gaaaatgaaa gagcggtttc ttctcgatcg gatcgatgtt catggcgcag
     3541 gccctgctgt gcacaagagt ataatattcg ccgccctggt tctcacggac tccgcagtat
     3601 cccctttcga gatcggttac aaggcaatga cgcgggcata actcacattc gatggcagta
     3661 ccctcgagct tcttgtaata catggccttg acatgcgaaa gagtatcggt tgtctccagc
     3721 gcataagcat tctggaacag cgccagcccg ttctcaaccg gattaaacgg caaacaggcg
     3781 catcccgccc cgcatgccat agctttgatc aatgtccttc ttttcataac ttaagttata
     3841 tcttcttcta tgatctccac ctgcgaaaga tcggattcgc ccaaacccag ccttttgcct
     3901 gtttccagcc agagcggctc acgtttgtcc tccttcaggc tcgggagtcc attctttcta
     3961 cgataatcct ctatgatttt atagccgtaa gcatcgatcg caatcggatc aaacgacata
     4021 accacagtcc ccggctttac cagatagcgt ggcatataac ccgggccacc gttatactgc
     4081 atcctggtca tatcgaggat cgtaagcaca tttttctctc tcacaatcgg aagcgccgac
     4141 agttcagcga catacggatt gcagttgtcc ggatgatatt tattgggatt atgaaccaga
     4201 ccatagaggt tcttaagccc gcccgacact cccgccacgg aatgatcttt tagaatcgga
     4261 aaattaatgt tcttatcgat ttcctgctcg aatattttag atatcagcga ggccaccttg
     4321 cctttttgat aaaagctgtc gctgtaaccc acacctctgg agtcagttcc gaaacaccgc
     4381 agcccgctgt tgcgtgcata attcaggtta tatccggccc tctcaagctc cctgttggtt
     4441 ctctcccaga tgatgatatc ggactcccgg agacccgccg attgcagacc tgcgactatg
     4501 gcatcggcca taaccgggga actggaaagc atgcgtccac tcagacaatt cggcttaatc
     4561 ccgaatctgt ccccctgggc ggcgattttt ttccaggcgg attttgaatc tgtcgatcct
     4621 gtgagacgta ccatggcatg actgatcaac ttatttgttt gatccctctt caaacc
//
LOCUS       DSEB01000318            9783 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000307, whole genome shotgun sequence.
ACCESSION   DSEB01000318 DSEB01000000
VERSION     DSEB01000318.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9783)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9783)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9783
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000307"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..864)
                     /locus_tag="ENO07_05770"
     CDS             complement(<1..864)
                     /locus_tag="ENO07_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01511.1"
                     /translation="MHYSPLQFARIFEEMKGRLKRAPGMDSKLLVLTNCWPDLQNDLQ
                     SKSYHGYLQEYASLLKHYLDAASLLDLEISEIRNIVSILIRLTKIDSKLGLDELNKLA
                     LKRLAMLYFYVGEVKSGLEACQGIMNREVDMSFEIDDTPGSSEYEYFDAVCKYYETHD
                     SGMHEILIQMRDEWKAKSTSLDYDYALCLFVEKGDSGRGVRGRMRTLKASLELASKAS
                     PDDKVSFDNQTKSPDDPFVGSVYNSLKAVRKVIGRYGHKEASKRFYNAHFSIENSKQT
                     FTGDSIGLAAGL"
     gene            complement(865..1719)
                     /locus_tag="ENO07_05775"
     CDS             complement(865..1719)
                     /locus_tag="ENO07_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01512.1"
                     /translation="MHPSRFEIEMYLAGRLDDDKKRAEIAEHLKLCEFCSEIAEEFRE
                     YLELLYEEDPEIPLRAYRQAELLANQALEGTIIPLKPIESACDEEIAYLAADGPEGEL
                     PEIVNLVTFYSDDPELVLKVMRNNREKRDYLELIGDEPFQVANVMIRIPEIEHDLITD
                     TNGIASLADLKNSQYEQFSWQIKMPDAVFHLEPLEYDPDKTEYSKEMELETDHGDKIR
                     IRFEGKTEGKQISVTIMQLEGESDFGGLKVLISQENLRLIKSISETGDVVFDLADKKA
                     GIDIRLFK"
     gene            complement(1747..2820)
                     /locus_tag="ENO07_05780"
     CDS             complement(1747..2820)
                     /locus_tag="ENO07_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01513.1"
                     /translation="MGNKYQDRIDRDSGMIMDFLRGPHKHFMQFYKLCFNHTRKHLCS
                     LQRRGAALPQIGSDPEKNLDDVTIDVLGVLLSSASGPYNIVFDYFAKHGINPDKKLDG
                     FLVYHRFTVLIRGFANQELYKIRGYENPQTRKIKRRFKDILKGSGYHESNIGGREFFT
                     LEATQNLREDKPVIPYEKLLQIVENAYHENISYVKWCRKIFEAIDKWGEYQNGIFRHE
                     LFQAAIEISSKHMEINFVERNNLSSIELALIRQAVERAIPKTLDWVSENASRGFVEKK
                     RITKEESQLFLKAIELYLRDFGCDGSPDKYPRYYFALFPGNKQDFYLKNHKYPFETMI
                     NSARDYFTNLLRNDSTINGIGDY"
     gene            3010..3219
                     /locus_tag="ENO07_05785"
     CDS             3010..3219
                     /locus_tag="ENO07_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01514.1"
                     /translation="MSDKEKVEAARRNYGFLEARRPVGNVGYLKFNYFTHLSIGGPTA
                     AAATQFLANTNAIINDLRGDRGGEV"
     gene            complement(4027..4299)
                     /locus_tag="ENO07_05790"
     CDS             complement(4027..4299)
                     /locus_tag="ENO07_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01515.1"
                     /translation="MIYRAGSRGYGKSELPKNQHGLLFMEFVTTGRIYYVDRKLLENP
                     VFEGNSAKSETSEVLQPQRLKFGSGGPLFDKVAEICPVIEIELQAK"
     gene            complement(4259..7357)
                     /locus_tag="ENO07_05795"
     CDS             complement(4259..7357)
                     /locus_tag="ENO07_05795"
                     /inference="COORDINATES: protein motif:HMM:PF00873.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDS01516.1"
                     /translation="MTLSEFALKNKHTVYALAIAAIFFGVLSYFSLPIQLFPETAPPL
                     VNILTPYPGAAAEDVADMVSDPIEEECAALEGVYKVSSSSQDGLSLVTVEFRYDVDVD
                     MAALDAQNAISRIREQLPAGIQEPQVLKFSTSDRPVLTVGITGENLTDVRRLAEEKLA
                     PELQRVEGVALVDVFGGHEPELSVLLDRNRLEAYSLPIAAVANAIKSHNISVPAGKIN
                     SEESQYSFRIDEESKTIQDIENIPIPLHDGSRLLLRDVAAIRYGSAEDLSKYHVNGQA
                     AIAVQIFKQDDANTVDVVSNAQEKFDELDHRYPQLEFIEAEESASFTRQVVSNMFGSV
                     AQALLLAAVIIFLFLGSLRRAVVVAISMPMSFLLTFAGMKFFGIQIDLVTLTAIILAV
                     GMVIDASVVVLENIARRYQLEDLTPEEAARVGASEIQFAIIAGNATTLTVLIPLLFLY
                     GFVGKTFGPLAATLIIAFLSSLLVALVMVPILTTFTAGEGGRFERLSRKISGPWNWLM
                     DRVRMAYLFLLTYSLSHRWAVFVSAVILLFAGLLLLRNQGTELLPKMDSGTSFVTVET
                     TSGTSLDHTETIVKEVEEIILEEPGVLLVSTQIGFEPGMHSFGGGGVQGPTQGYISIT
                     LSPRTDRNETIWEIQDRIREKIKKIPGIENLVVRESGSTARSTTASSIVVSIRGDDPL
                     VLDQLGDQVLNAISIVPSVVNPYRSWRRDQRAVYLNVNRERALDLAMTPLGLSRDLTY
                     SLDGVMAGTFEGQDGEQTPIRVRYDRRYRATEPDAYAVRAISPAGETSIPVSAIINGR
                     EATVQGLVTRENLQPTLDILALHQGRPLNFVAADVSKAVNELTIPQGYTAELLGEDKD
                     MAESRSELVFALVIAFVTVYLLLVAQFRSFLHPVTVLMSIPLSLIGVGIALWVAGKAV
                     SMPVMVGLILLIGILVNNSIILIDLIRQRRIDGVGRREAIIESVETRFRPIMMTSFST
                     IVGMIPLAMEWALGAERFSPLAIAVIGGMTASTLLTMIVIPIIYDWLDDLQGWFSRIW
                     KK"
     gene            complement(7363..8439)
                     /locus_tag="ENO07_05800"
     CDS             complement(7363..8439)
                     /locus_tag="ENO07_05800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDS01517.1"
                     /translation="MKGLATLIIGVSITPLRHAAIVLMMFSLAFLTGCSGESESPKAD
                     APVAVRVTSPQVRDMTDHVTYTGIVRARQEYTVIARVQGTVGSLPIAEGKAIHTGDTL
                     VALSTPELEAVVARLEAEFEYWDERLAEDKRLEAKGAISREQVDASKRAFASSKAALQ
                     EAESQLAKATELASFDGYMLKHFVDPGQTVMPGQPLLQIGSDELEIQAEVVQEDIGPS
                     IKPGTVAVVHDSEGAQHRTRVAQVASMTSKKSRTFTVTVSLAPSFGEQYRVGEAVVVD
                     FIVKSSEQVLSVPVTALLNTGTDPAIYLIIDNRAVRTPVSVGIQQSEYVAVQFDWNGS
                     DPVAISNIAGLSDGDPVYPVYVER"
     gene            complement(8436..9776)
                     /locus_tag="ENO07_05805"
     CDS             complement(8436..9776)
                     /locus_tag="ENO07_05805"
                     /inference="COORDINATES: protein motif:HMM:PF02321.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDS01518.1"
                     /translation="MKHHYIFTYLIMAAVLLASLAPASGQRAVTVSEAIDSSLAFNPQ
                     LLMTDAKLDASEATVRQATGSLLPQLYATAGYTRYEEPNIVTPIHEQGVFPPLDDEIY
                     EANLQLSIPIFDGGRRLTRRKAASADVDESRATDDLVRNEILQQVAEIFLYARRAEDQ
                     SALINKRLTSLYRQLHDLQSLEKEGRASKGDLALVSSLIASLKSDSSAIARSRHQLGI
                     RLSALMGTDDEVTPAISENGMDLEALIQSYPLTDFEADGQSGPSVRVSEARLKKAGAY
                     QSLASRELWPEISGFGVYSYRTGSDWDPVGEWTVGIKVSLPLFTGGSRISNIEEAASL
                     RRASENALRSAKLEQQSILQAAYNDYISAANRRDYLSTAVKEKLISVQAHLDLYEAGR
                     IPLRDLHVQETELLQLQIEKNAQQYEARLALLRYEAAAGTLTQSKVISLTGGVQ"
BASE COUNT         2546 a         2512 c         2190 g         2535 t
ORIGIN      
        1 cagccccgca gccaggccga ttgaatcgcc ggtgaaagtc tgcttgctgt tctcgataga
       61 gaaatgtgca ttgtaaaatc gtttgcttgc ttccttgtga ccatacctgc caattacttt
      121 tcgcacagct ttcagtgagt tataaacaga accgacaaat ggatcatccg gcgacttggt
      181 ctggttgtca aaggaaacct tgtcatcggg agaagctttc gatgccaatt ccagcgaggc
      241 tttcagcgtt cgcatgcgcc cgcgcacgcc cctgccggaa tcaccttttt caacaaataa
      301 gcagagcgca taatcataat ccaggcttgt cgatttagcc ttccattcgt ccctcatctg
      361 aatcagaatc tcatgcatcc cggaatcgtg tgtttcgtag tacttgcaaa ccgcatcaaa
      421 atattcatat tcgcttgaac cgggggtgtc gtcaatttca aagctcatat cgacttcccg
      481 attcatgatg ccctgacatg cttcaaggcc tgatttaact tccccgacat aaaaatagag
      541 catcgccagc cttttaaggg ccagcttgtt caattcatcc aggcccagtt ttgaatcaat
      601 ctttgttaat cggatcaaaa tagagacaat attgcggatc tccgaaatct ccaggtccag
      661 cagtgaagcg gcatccagat aatgtttcag gagggaagca tattcctgca ggtagccatg
      721 gtaagacttg ctctgcaggt cattctggag atcgggccaa caattagtca aaaccagcag
      781 ttttgaatcc atgccgggcg cccgcttgag cctgcccttc atctcctcaa agatccgagc
      841 gaattgcagt ggggagtaat gcatttactt aaaaagcctt atgtctattc ctgctttttt
      901 atcagcaaga tcgaaaacca catcgcctgt ttcagatatt gactttataa gtctcaaatt
      961 ttcctgggaa ataagaacct tgagtcctcc gaaatccgat tctccttcca gctgcattat
     1021 cgtgacgctg atctgtttgc cctcggtttt gccttcgaac ctgatcctga ttttatctcc
     1081 atggtctgtt tcaagttcca tctctttgga atactctgtt ttgtcgggat catattcaag
     1141 tggctccaga tgaaatacag catcgggcat tttgatctgc caggagaatt gctcatattg
     1201 gctgtttttt aaatccgcca aagaagcgat tccgtttgta tctgtgatta agtcatgttc
     1261 aatttcgggg attctgatca taacgttagc tacctgaaat ggctcatcgc ctatcagctc
     1321 cagataatcg cgtttctccc ggttattccg catgaccttc aataccagct cgggatcatc
     1381 ggaataaaat gtgaccagat taacgatctc cggcagctca ccctctggcc cgtcagccgc
     1441 aaggtaggct atctcctcgt cacaggcaga ttcaataggc tttaaaggta taatcgtgcc
     1501 ttccaatgcc tggttagcca gcaactcggc ctgccgatag gcgcgaagag gtatttcggg
     1561 atcttcttcg tacagcaact caagatattc cctgaattct tcggcaatct cgctgcagaa
     1621 ctcgcatagt ttcaaatgtt ctgctatttc cgctcttttt ttatcgtcat caagccttcc
     1681 ggccagatac atttctatct caaagcggga agggtgcaca aatcaccgcc tttctccaca
     1741 catatatcaa tagtccccaa ttccattgat tgtagaatca tttcttaata aatttgtgaa
     1801 ataatccctg gcagaattaa tcattgtctc gaatggatat ttatgatttt tcagataaaa
     1861 atcctgttta ttgcccggga aaagcgcaaa atagtagcgg ggatatttat caggagaacc
     1921 atcacagcca aagtcccgga gatataattc aatcgccttc aaaaataatt gggattcctc
     1981 tttggttatt ctcttttttt ccacaaatcc cctactggca ttttcactaa cccaatcaag
     2041 agtctttgga atcgccctct ccacagcctg cctgatcaat gctaactcga tcgaggatag
     2101 attgttgcgc tcgacaaaat taatttccat atgcttgctg cttatttcaa ttgctgcctg
     2161 gaaaagttca tggcggaaaa tgccgttttg atattctccc catttatcta tcgcttcaaa
     2221 tatctttcgg caccatttga catagctgat attttcgtgg taagcgtttt ctacaatctg
     2281 caaaagtttc tcgtagggta taaccggctt gtcctcccga agattctggg tagcttcaag
     2341 cgtgaagaat tctctaccac caatattgct ctcgtgataa cctgagcctt tcaatatatc
     2401 cttgaaacgc cttttgattt tcctggtctg gggattttcg taaccacgga ttttatataa
     2461 ctcctgattg gcaaaacctc taatcagcac tgtaaatcga tggtagacca aaaaaccgtc
     2521 tagcttctta tcaggattaa tgccatgttt cgcaaagtaa tcgaatacta tattgtatgg
     2581 cccactggcg ctgctgagca acacaccgag tacgtctatt gtcacatcat ccagattctt
     2641 ctcagggtcg gatcctattt ggggaagcgc ggccccacgg cgctgaagag agcagagatg
     2701 ttttctcgtg tggttaaagc agagtttata gaattgcatg aaatgcttgt gcgggcctct
     2761 caagaagtcc atgatcattc cggaatcacg gtcaatcctg tcttgatact tgttccccat
     2821 caggtatgat ccccaattat gtgccctgac atgaagcccg ccgcgtcaaa ttaaacttat
     2881 atggtcgtca taaatatact aaaatcagaa tgagtgtcaa tagttgacat taagagatct
     2941 taatggtgcg gatagaatcc tatactcgtg cgcggcacat tcaatttcct caaggctgga
     3001 ggttatgcca tgtcagataa ggaaaaagtc gaggctgcca ggcgcaatta tgggtttctt
     3061 gaggcaagaa gaccggttgg gaatgtcggt tatcttaagt tcaactactt cacacacctg
     3121 tctattggcg gcccgaccgc tgcggccgcc acgcagtttc tggccaacac taatgctatt
     3181 ataaacgacc ttcgcggtga taggggaggc gaggtgtaaa aagatggtta gaagtctatt
     3241 ctccgaattt aagccggatt atgtcctgca tcatccgcat accaccgtca agacaaggat
     3301 ctggattgct gcctggaaga ctctagaaat ggaagctagt acagatatta aatgggccag
     3361 tgcagggaag ttctgcgaag cgcatcgtga caagagcgat tatgataata tgattggcgt
     3421 gatgcaggcg agcaaaagta agaacataag ctctatggat ttgatatgtt ccaaattagg
     3481 cacatatttt ccgattaagt ctgacctatt gcgtcttaat aatcaatatt acatacagaa
     3541 tcgaatctag aatacccgtg aaagacttcg agcacagtca attccaaaca gcttctatat
     3601 aaattctcgg agggatggga acccgaattg ttgtgaaccc cctgctttgt aggggcatgc
     3661 caggaaccgc gcacgctata gtacatgaac ttcacacctg tgatttgggg gctggtcttt
     3721 atgatctctc acacttttgt gatgggattc tccactggag aaggtcattc cattccccgc
     3781 tgtctcggat cgagaaggat gaatggtcca cttttcaatc ctatggtttg ggatgggttg
     3841 tccaaaatca tgctgcacgt gttgggcagg atgcagtttt ctgagattgt ctagaagtga
     3901 agaataccca tcaatcggaa agaattgcca cagaagctgt atttttggcc cccgcagagc
     3961 caaaatcagt cgagatcatg gtgaaaacaa agaaagagaa tgaacgaaca agcctttatc
     4021 aaatattcac ttagcttgaa gctctatttc tattacaggg catatctctg caactttatc
     4081 gaaaagcggg cccccgctac caaatttaag cctctgaggt tgaagaactt cggaggtttc
     4141 actttttgcg gaattaccct cgaataccgg attttctaac agttttctgt caacatagta
     4201 aattcttcct gtggtaacaa attccatgaa aagtaaaccg tgttgatttt tagggagttc
     4261 actttttcca tatccgcgag aaccagccct gtaaatcatc cagccagtca tatatgatcg
     4321 gaatcacaat catggtcagc agggtagagg ctgtcatgcc tccgatgaca gctatggcaa
     4381 gcggcgagaa cctctctgct ccaagcgccc attccatggc aagtggaatc atacccacga
     4441 tggtcgaaaa cgaagtcatc ataatcggcc tgaagcgggt ctcgaccgat tcgattatag
     4501 cttcccggcg gccaacgcca tcaatacgtc gttgacggat taaatcaatt aagataatcg
     4561 agttgttcac caggatgccg atcagaagaa tcagtccaac catcaccggc atcgagactg
     4621 ctttaccagc tacccacaag gcaatgccca cgccaattaa actgaggggg atgctcatca
     4681 ggactgtaac ggggtgcaga aatgatcgga actgtgccac caataataga tacacggtga
     4741 caaatgcaat aaccagcgca aaaaccagct cgcttctgga ttctgccatg tctttgtcct
     4801 ctcccagtaa ttccgccgta taaccctgcg gaattgtcaa ctcattaaca gctttgctga
     4861 catcagcagc cacaaaattc agcggtcgcc cctggtgaag tgcgagaata tcaagagttg
     4921 gttgtaaatt ctctctggtc acaaggccct ggacagtcgc ctcccggcca ttaataatcg
     4981 cacttaccgg aattgaagtt tccccggccg gcgatatcgc tctgaccgca tacgcatccg
     5041 gttccgtcgc gcggtagcgc cggtcatatc tgaccctgat cggagtctgc tcaccgtcct
     5101 gaccctcaaa agttcccgcc ataacaccgt ccaaagagta ggtcagatcc cgcgaaagac
     5161 ccagcggtgt catcgccaga tcaagcgcac gctctctgtt gacatttaaa tagacggcac
     5221 gctggtcgcg acgccaactc ctgtatggat tgaccaccga gggaactatg ctaatggcat
     5281 tcaatacctg gtcccccagt tgatcaagaa ccaggggatc gtccccccga atactaacca
     5341 cgattgagga agccgtggtt gaccttgctg tgcttccgga ttcgcgcact acaagatttt
     5401 caataccagg aatttttttt atcttttctc taatacggtc ttgaatttcc caaattgttt
     5461 cattgcggtc agtacggggg gaaagtgtta tcgatatata accctgtgtc ggaccctgga
     5521 caccgcctcc cccaaaggaa tgcattccag gctcaaaacc aatctgggtt gaaaccagaa
     5581 gtactccggg ctcctcaaga attatttctt caacctcttt aacaattgtc tctgtatgat
     5641 ccagtgaggt tccggaggtt gtctcgacgg tcacgaagct cgtcccgctg tccatcttgg
     5701 gcagcaactc agttccctga ttcctcagaa gcaaaagacc ggcaaagagc aatatgacag
     5761 ccgagacaaa gacagcccag cgatgtgaga gggaatatgt taatagaaac aggtaagcca
     5821 ttcggacccg atccataagc cagttccacg gtccggatat cttgcgggac agcctctcaa
     5881 aacgtccgcc ctcgcccgcg gtaaaggtgg tcagaattgg aaccataacc agagcgacca
     5941 acagcgatga caagaaagca attattaaag tcgcagccag aggaccaaaa gtctttccga
     6001 caaatccgta caagaaaagc aaaggaatca gaactgtcag cgtcgtcgca ttaccggcga
     6061 taatagcaaa ctggatttcc gaagctccga cccgagccgc ttcttcggga gtgagatcct
     6121 ccagctgata tctccttgca atattttcca gaactacaac agaggcatcg ataaccatgc
     6181 caacagccag gataatcgct gtcagcgtaa ccaggtcgat ttgaatcccg aaaaatttca
     6241 tgcctgcaaa agtaagcagg aaagacatgg gcattgatat cgcaaccacc acagccctgc
     6301 gcagacttcc cagaaaaaga aatatgatta cagccgcgag caacagcgct tgcgcgacac
     6361 ttccaaacat gttgcttaca acctgtcgcg taaacgaggc cgattcttca gcttcaatga
     6421 actcgagttg cggatatcgg tgatccagtt catcgaactt ctcctgagca ttcgaaacta
     6481 catcgacggt attcgcatca tcctgcttga atatctggac agcaatagca gcctggccgt
     6541 taacatggta cttggacagg tcctccgcgc tgccataacg aatcgcagcc acatcacgca
     6601 acagaagtcg gcttccatca tgaagcggga tgggaatatt ttcaatatcc tggattgtct
     6661 tgctctcttc gtcaatgcga aacgaatact ggctctcttc ggagttgatt tttcctgcgg
     6721 gaacactgat gttgtgggat tttatggcat tggcaacagc cgcgatgggc aggctgtatg
     6781 cctccagccg gttgcgatca agcagcaccg atagttcagg ttcatgaccc ccgaaaacat
     6841 ccaccagcgc aaccccctcc acacgctgca gttcaggagc aagtttctcc tcggctaacc
     6901 tgcgcacatc agtaagattt tcacctgtaa tgccaacagt caaaaccggc ctgtcgcttg
     6961 tagagaactt taagacctgt ggctcctgta taccggctgg caattgttcc cggattcgag
     7021 atatcgcgtt ttgcgcatcc aatgctgcca tatcgacgtc aacatcgtac cggaattcaa
     7081 ccgtcacaag tgataatcca tcctgcgacg aagaggatac cttatagact ccttccaagg
     7141 cagcgcattc ctcctcgatc ggatcagaga ccatgtcagc aacatcttca gccgcggctc
     7201 cggggtatgg tgtcaatatg ttcaccagcg gcggagccgt ttcggggaag agctggatcg
     7261 gaagcgagaa atacgacaaa acaccgaaga atatcgcggc aatcgccaac gcgtatacgg
     7321 tatgtttatt tttcagagca aattctgata atgtcatggt tactatctct ccacataaac
     7381 cgggtataca ggatcgccat cagagaggcc ggcaatgttc gaaattgcca ccggatcaga
     7441 gccattccaa tcgaactgca cggcaacata ttcagattgc tgaattccca ccgatacggg
     7501 agttctgacc gcacgattat caattatcag atagatggct ggatcggtgc ctgtgtttaa
     7561 cagtgcagtt accggaacgg aaaggacctg ttccgaactc ttcactataa agtcaaccac
     7621 aacagcctcg ccgacgcggt attgctcacc gaatgacggc gccaatgaaa ctgtaactgt
     7681 aaacgtacgg ctcttcttgc tcgtcatcga tgcgacctgt gccacccggg tcctgtgttg
     7741 cgcgccttcg ctgtcgtgaa caacggcgac cgtaccgggc ttgatgctcg ggccaatgtc
     7801 ctcctgaaca acctcggctt gaatttcaag ctcgtcactg ccgatttgaa gaagcggctg
     7861 tccgggcatc acggtctggc cgggatccac aaagtgcttg agcatatagc cgtcaaatga
     7921 tgccagttca gtagccttcg ccagctgtga ttcggcttcc tgtaaggcgg ctttggagct
     7981 tgcgaaagcc ctctttgatg catctacctg ttcacgtgaa atcgcccctt tggcctccag
     8041 tctcttatcc tctgccaatc tttcgtccca gtattcgaat tccgcctcca gtctcgccac
     8101 aaccgcttca agttccggag tagataatgc aaccaaagtg tcgcctgtat gaattgcctt
     8161 accttcagct atcgggagag agcccacggt tccctgcacc cgcgcgataa cagtgtactc
     8221 ctgtctggcc cggacgatac cggtataggt tacatgatcc gtcatatccc ttacctgcgg
     8281 actggttacc ctcacagcca ccggagcatc cgctttcggg ctttcgcttt cccccgaaca
     8341 gcctgtcaag aacgccagtg aaaacatcat aagcactatg gctgcgtgcc tgagcggcgt
     8401 tattgagact cctattatta aagttgctaa acctttcatt gaactcctcc tgtcaaactt
     8461 atcaccttgc tctgagtaag agtcccggcg gcagcctcgt aacgcaggag cgccagacgc
     8521 gcctcatatt gctgagcgtt tttttctatc tgcaactgga ggagttctgt ctcctggaca
     8581 tgcaaatcgc gcagcggtat tcgcccggcc tcatacaggt caagatgagc ctgcacagaa
     8641 atcagctttt cctttacagc cgtagataaa taatcacgcc tgtttgctgc cgatatgtaa
     8701 tcgttatatg ctgcttgaag aattgattgc tgttccaatt ttgcgcttct gagagcattt
     8761 tcagaggcgc gcctgagtga ggcagcctct tcgatatttg agattcttga tccccctgta
     8821 aagagcggca aagagacttt tatgccaacg gtccattcac ccaccggatc ccagtcggaa
     8881 ccggtacggt aggaatagac tccaaaaccg gaaatctcag gccacaattc tcgagatgct
     8941 aaggactggt aagccccggc ttttttaaga cgagcctcag aaacacgtac actgggcccg
     9001 ctctgcccat cagcctcgaa atctgtcagc ggataagatt gaataagtgc ctcaaggtcc
     9061 attccgtttt cagagattgc cggtgtcacc tcgtcatccg tgcccataag agctgaaagc
     9121 cgtattccaa gctgatgccg cgatcgtgca attgcgcttg agtccgattt cagtgaagct
     9181 atgagggagg ataccagagc aagatcacct ttggacgctc gaccttcctt ctccagactt
     9241 tgcaggtcgt gcaattgccg atacagggaa gtcagtcgtt tgttgatgag cgccgactga
     9301 tcttcagccc ggcgggcata taagaatatc tcagcaacct gctgcaatat ctcattccga
     9361 acaagatcat ctgttgcccg gctttcatcg acatcggcag aggcagcttt acgcctggtt
     9421 aagcggcggc ctccatcaaa aataggtatg gacaattgga ggttggcctc ataaatctca
     9481 tcatcaagcg gcgggaaaac accctgctca tggattggag taacaatatt gggttcctca
     9541 tatcgggtgt atccggccgt agcgtaaagc tgcggcagca gcgatcctgt tgcctgacga
     9601 accgtggcct cagaggcatc cagcttagca tctgtcatta gaagttgagg attaaacgcc
     9661 aaagaggaat cgatcgcctc cgagacagtt actgctcgct gacctgaagc tggcgccagt
     9721 gatgccaaca gcacagctgc cataatcaga tatgtaaata tataatgatg tttcattctc
     9781 ttc
//
LOCUS       DSEB01000319            1856 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1011995, whole genome shotgun sequence.
ACCESSION   DSEB01000319 DSEB01000000
VERSION     DSEB01000319.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1011995"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            58..708
                     /locus_tag="ENO07_05810"
     CDS             58..708
                     /locus_tag="ENO07_05810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HDS01519.1"
                     /translation="MDRKPNAVLPAAILGTADFDVSEIDPATLMLEGVPVLRWSIEDV
                     GTPVNPDAPECECNTLGGDGYTDLTLKFDMEGVVATLGEVYDGNEIALNISGQLYDGT
                     EITGSDCVIIRAERAVDGIFARSNEVPESFELKQNYPNPFNPETEIVFDLPKGCQVKL
                     EIFNITGQRVAVLANRYFDAGSYSVIWNAVGQTSGVYFYRIATDNYIESKKMILLK"
     gene            982..>1856
                     /locus_tag="ENO07_05815"
     CDS             982..>1856
                     /locus_tag="ENO07_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01520.1"
                     /translation="MFRKPSLLCLAVVITFIGLTTIHGRAENVCGDVNNDAEVNVSDA
                     VWTANYIFIGGPEPVPFLSGEVNCDGSVNVSDAVWIANYVFTGGNDPCDSDGDGIPDC
                     HPDCPPTVTDIDGNVYQTVLIGDQCWMMENLKVTHYRNGDPIPEVTDGGSWGMLSTGA
                     YCNYDNNESYVAVYGRLYNWYAAADSRNIAPDGWHVPSDAEWKQLEMYLGMSQADADA
                     TGWRGTDEGGKLKEADTTHWQSPNNGATNESGFTALPGGYRSSSGRFYFEHFDAFFWT
                     QTDYDILTVWYRYLN"
BASE COUNT          479 a          399 c          488 g          490 t
ORIGIN      
        1 ccttatagat cggaggattt gaccgcggcg aatgtatccg aaaagttggt tccatatttg
       61 gatcggaaac ctaacgccgt cctgccggcc gctatcctgg gaacagccga ttttgatgtc
      121 tcggaaattg acccggctac attgatgctt gaaggagtcc ctgtcttaag gtggagtatt
      181 gaagatgtgg ggacaccggt caacccggat gcgcctgaat gcgaatgtaa tacattggga
      241 ggtgatggct ataccgatct aaccctgaaa ttcgacatgg aaggcgtggt ggctactttg
      301 ggagaggttt atgacggaaa tgaaattgca ttgaatatca gcggtcaact ttacgatggc
      361 actgagatta ccggttccga ctgtgtgata attagagcgg aacgggcggt tgatggcata
      421 ttcgcacgtt caaatgaggt gcctgagtcg tttgaactca aacagaacta cccaaatcca
      481 ttcaatcctg aaacagaaat agtatttgac cttccaaaag gctgccaggt caaactggag
      541 atattcaata ttaccggcca gagagttgct gtgctggcca ataggtactt tgatgcaggc
      601 agctattctg taatttggaa tgccgtcggg caaacgtccg gagtttattt ttatagaata
      661 gcgacagata actatattga gtccaagaag atgattctgt tgaagtaaca ctatttaagg
      721 aagtccaaga cctgttcgtg cgcggcgtac ctccggtgcg ccgcgttttt gtggatcttc
      781 ttatatagat caacatctga acgttaaaac accgggaaac cggttagtca acgtctcatt
      841 tgggatcggc tgacaggcag aaaatcattg acagtttaat gcatttttat atatttcgcc
      901 aagcttacca gatcgcagct ttgggccaaa tctgtaatga atgattgtat ttaaaatatc
      961 cataagcgga ggcatatcat aatgttcaga aaaccctctt tgctctgcct tgcagtggtg
     1021 attacattta tcggcttgac caccattcat ggacgtgctg agaatgtttg cggggatgtc
     1081 aacaatgatg ctgaggttaa tgtcagtgat gcggtctgga ctgctaatta tatttttatc
     1141 ggcgggcctg agccggtgcc attcttatcg ggagaagtca attgcgatgg ttccgttaat
     1201 gtcagcgacg ctgtctggat agccaattac gtattcacgg gaggcaatga tccatgcgat
     1261 tccgatggtg acggcattcc ggactgccac cctgattgtc ctccaacagt gaccgatata
     1321 gacggcaatg tttaccagac agttctgatt ggtgaccagt gctggatgat ggaaaatctg
     1381 aaagtaacac actatcgtaa cggtgatccg attcctgagg tgaccgacgg aggcagctgg
     1441 gggatgcttt caacaggcgc ctactgcaac tatgataaca acgagagcta tgtagctgtc
     1501 tatggccgat tatacaactg gtatgccgcc gctgacagcc gcaatattgc gccggatggt
     1561 tggcatgtcc ccagcgatgc cgagtggaaa cagttagaga tgtatctcgg catgagccag
     1621 gctgatgccg atgctacggg ctggcgcggt accgacgagg gcggaaagtt gaaggaagct
     1681 gatacaaccc attggcagag ccccaacaat ggcgctacca acgaaagcgg ttttactgcg
     1741 ctacccggcg gctaccgcag cagctccggt cgtttttact ttgagcattt cgatgctttt
     1801 ttctggaccc agacagatta tgatattctg acagtgtggt atcgctatct taatta
//
LOCUS       DSEB01000320            2122 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1008878, whole genome shotgun sequence.
ACCESSION   DSEB01000320 DSEB01000000
VERSION     DSEB01000320.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1008878"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..507
                     /locus_tag="ENO07_05820"
     CDS             <1..507
                     /locus_tag="ENO07_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01521.1"
                     /translation="TDDIEWMELTAAAYWDYDNDDANSDVYGRLYNWYAIDDPRGLAP
                     EGWHVPTDEEWKQLEMYLGMSQAEADATGWRGTDEGGKLKEAGTIHWYAPNTGATNES
                     GFTALPAGMRHPFALFEGLFLYTTFWTSTEDSDFSAFIRQLYNNYEQVWRFLFDKKSG
                     CSVRCVKD"
     gene            complement(586..1419)
                     /locus_tag="ENO07_05825"
     CDS             complement(586..1419)
                     /locus_tag="ENO07_05825"
                     /inference="COORDINATES: protein motif:HMM:PRK06181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDS01522.1"
                     /translation="MSAKVFKDNVVIITGASSGIGQELALQLADQGAWLVLAARSEER
                     LEEIATECRNRGGKALVVTANVAREEECKKIIEETLEEYKRIDTLINNAGYGSSGHLS
                     DLPDLRGFENVMDVNFRGSVYCTYYALPHIIRMRGRIVGISSVLGKVAAWGSTGYCAS
                     KFAMAGFFDALRHELHDRYVSVTVIYPGLVVTNFAGNIIQPSGEKSGEAGLRFYNNRM
                     MSAEKAAKLIIEAAAKRKRQKVLSGLGKLVVFLNKLSPALVDRIGLKIQAVRRKRIEG
                     K"
BASE COUNT          516 a          524 c          515 g          567 t
ORIGIN      
        1 accgatgata ttgagtggat ggagctcacc gctgcagctt attgggatta tgataatgat
       61 gatgccaact ctgatgtata tggcagatta tacaactggt atgccatcga tgaccctcgc
      121 gggttggctc cggagggctg gcatgtgcct actgatgaag agtggaagca gctggagatg
      181 tatcttggaa tgagccaggc agaggccgat gccacgggtt ggcgtgggac tgatgagggc
      241 ggcaaattaa aagaggcagg cacaatccat tggtatgcac caaatactgg cgctacgaac
      301 gaaagcggtt ttacagcatt gccggcagga atgcgccatc cctttgcgct gttcgaaggg
      361 ctatttctct ataccacctt ctggacctcc accgaggaca gtgacttttc agcatttatt
      421 cgtcagcttt acaataatta cgagcaggtc tggcgtttct tatttgataa aaaatccgga
      481 tgttcagttc gttgtgtcaa agattgattg tttgaatccg atattaatac ccggcggagc
      541 acattgaatt ttcgtgcgcg gcgtacctcc gggggcgccg cgattctatt tgccctcgat
      601 ccttttcctt cttacggcct ggatcttaag gccgatgcgg tcaaccagcg caggagagag
      661 tttatttaag aacactacca gcttgccaag tccgctgagg actttctgtc gtttgcgctt
      721 agctgcggct tcgataatga gcttagcggc tttctcggcg gacatcattc tattattata
      781 aaatcgcagt cccgcctcgc ctgacttctc accgctcggt tggataatat ttccggcaaa
      841 attcgtcaca acaagtccgg gatatatcac tgtcacagaa acatatctgt catgcagctc
      901 atggcgcagg gcatcgaaaa atccggccat ggcgaactta ctggcacaat aacctgtact
      961 gccccatgcc gcaaccttgc cgagcacact ggagatgccc actatgcgac ctctcattct
     1021 tataatatgc ggcagggcat agtaggtgca gtaaaccgaa ccccggaagt tgacatccat
     1081 gacattttcg aagccacgca gatcgggaag atcgctcaga tgtcctgatg agccgtaacc
     1141 cgcattattt atgagggtat caatacgctt atattcttcc aatgtctcct cgatgatctt
     1201 cttgcactct tcctcgcgcg ccacattggc tgtaaccaca agcgctttac cgccgcggtt
     1261 tctgcattcc gtggcgatct cctccagccg ctcttcgctc ctggcggcaa ggaccagcca
     1321 ggcgccctgg tcggcaagct gcaatgccag ctcctggccg attccgctcg aggcgccggt
     1381 tatgatgact acattgtctt tgaatacttt tgccgacata taattacccg ggtataagca
     1441 aagatatcat gttctattgt cgtgaatgta agcaaagaat gataattaat gcaagagatt
     1501 tataagtaat gatgtgcagt ggcaaaggtc attcttgagt ttcttcgctg taaacaccgg
     1561 tctgcatgac ctcgataatg cccagatcat tgtaggtgat ctctactgtc ttggaggtgt
     1621 ccagagttaa cgggtctgtc agaatcgtct ttgtgctggc caccggcagg atccgtttca
     1681 ggtctttgat tagcccgatc atgctctttt cttcatttcc cagttgagtg gtcctgaact
     1741 ggtcatcact gtatatgtaa tcctcgatat agattacccg ggagaagcga tataattcat
     1801 ccaggcccag gagattcccg agcctcctga acttgatgac ttccatctcg atttgaaatg
     1861 cgctgtcatc cagatagact tcatctgcca ggagaggacg atttctttca tcgaaagtct
     1921 gcaggaccag aagcggcttc tcactctcgg actgactgaa cgatatttgc gccaggggaa
     1981 tattgcggtt caatacaaaa tagttcttca aactcgaatt aaaccagaag aacgactggg
     2041 aaacaagaat caggattaag cccgaaacga tcagaatggc gcgtacccat ttattcgcct
     2101 ttttgggggc atatccctga ga
//
LOCUS       DSEB01000321            2488 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1006246, whole genome shotgun sequence.
ACCESSION   DSEB01000321 DSEB01000000
VERSION     DSEB01000321.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1006246"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1517)
                     /gene="topA"
                     /locus_tag="ENO07_05830"
     CDS             complement(<1..1517)
                     /gene="topA"
                     /locus_tag="ENO07_05830"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:PRK05582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDS01523.1"
                     /translation="MGVKKKKRTGSKTGSKAATDLLVVESPTKTKTLKKFLGKKYKVM
                     ATKGHIIDLPKSRLGVDVDDDFTPNYMVIRGKKKVIDEIAREARKSGNVYLAPDPDRE
                     GEAIAYHLYGRLSGVNNNIYRVSYNELTKSAVKKAMDNAGPINDDKVMAQQARRILDR
                     LVGYKVSPILWKTVLRGLSAGRVQSVALKILCEREEEIEKFEPEEYWKVKAELLSGKE
                     NKFYAMLHKIEGEDFKIGSEKESDEICNDIRQQEFKIASVKKEKKKRNPYPPFITSTM
                     QQDASSRLRFAPQKTMYIAQQLYEGIDLGKAGSVGLITYMRTDSVRLSNEALGQARSY
                     IKERYGEKYLPDSPRVYKSRKSSQEAHEAIRPTSLEHHPDEIKEFLTRDQYRLYNLIY
                     ARFVASQMVPAVYENISVDIEAGKYLFRAGTSQLVFDGFLKVYKAANGNGKDNGEDEP
                     QVLPELEEGEKLKLEDLLPTQHFTKPPPRFSEASLVRELEQNGIGRPSTYAQIIYT"
     gene            complement(1507..1734)
                     /gene="rpoZ"
                     /locus_tag="ENO07_05835"
     CDS             complement(1507..1734)
                     /gene="rpoZ"
                     /locus_tag="ENO07_05835"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDS01524.1"
                     /translation="MGGSMARVKLDELDKVIKNRYAAVIVAAKRARRINAERVAKLEL
                     MPENDEIDIDPRKVTTRAIEELIDGKIKIGR"
     gene            complement(1743..2354)
                     /locus_tag="ENO07_05840"
     CDS             complement(1743..2354)
                     /locus_tag="ENO07_05840"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:PRK00300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDS01525.1"
                     /translation="MSTRKKYIKGPMLVVISAPSGAGKTTICRGLLKRHDNIRMSISA
                     TTRPKAKGEREGRDYFFMKNEEFETKLEQGWFVEWARVYGHYYGTPVEQVEKYLAQKK
                     VVLFDIDVQGGRAIKKSYPDAAAIFILPPSLNELKRRLKLRKRDSREEMDKRLALALK
                     EIEMRTKYDYTVVNADVDTTVSQIESILEAESLKTSRNVVTKI"
     gene            complement(2355..>2488)
                     /locus_tag="ENO07_05845"
     CDS             complement(2355..>2488)
                     /locus_tag="ENO07_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HDS01526.1"
                     /translation="ILQELYREANTIGSKSISPEISRNVIMMKEEIEKVREQVQNLE"
BASE COUNT          535 a          676 c          536 g          741 t
ORIGIN      
        1 gtatatataa tctgtgcata agtcgacggg cgcccgatac cgttttgttc cagttcacga
       61 accagactgg cctcggaaaa cctcggcgga ggttttgtga aatgctgtgt cgggagcagg
      121 tcctccagct tcagcttctc tccctcttcc agctccggca gtacctgggg ttcatcctca
      181 ccattatcct tgccattacc attggcggcc ttgtacactt tcaggaagcc gtcgaaaaca
      241 agctgcgagg tgccggcgcg gaaaagatat ttgccggctt ctatatctac tgagatattt
      301 tcatagacgg ccggtaccat ctgcgaggcc acaaagcggg catatatgag attatagagg
      361 cggtactggt cgcgggtcaa aaattcttta atttcatcgg gatgatgctc gagcgatgtc
      421 ggacggatcg cctcatgcgc ttcctgggag gatttcctgg atttatagac ccggggtgaa
      481 tccgggagat atttttcacc ataacgttcc tttatatagc tcctcgcctg tcccagggct
      541 tcgttggaaa gccgcaccga atctgtacgc atgtaggtta tcaggccgac cgacccggcc
      601 ttgcccaggt cgataccctc gtaaagctgc tgggctatat acatggtctt ctgaggggcg
      661 aatcttagac ggctggaggc atcctgctgc atggtcgagg ttatgaaagg aggatatgga
      721 tttcttttct tcttctcttt cttgaccgag gcaatcttaa attcctgctg cctgatgtcg
      781 ttacagatct catcagattc tttttctgat ccgattttga aatcctcgcc ctcaatttta
      841 tgcagcatgg cataaaactt attctcctta ccggacaaaa gctcagcctt taccttccag
      901 tattcttccg gctcgaactt ttctatttcc tcttcgcgct cgcacaggat tttcagcgcc
      961 accgactgca cccgtccggc cgaaagaccg cgcaggacgg tcttccacag gatcggggaa
     1021 accttgtaac ccaccaggcg gtcgagtata cgccgcgcct gctgagccat aaccttatca
     1081 tcattgatgg gtccggcatt gtccatggcc ttcttgacag ctgatttggt gagctcgtta
     1141 taagatacgc gatatatatt attgttgacc ccgctcagcc tgccatacag atgataggca
     1201 atcgcctctc cctcacggtc agggtcgggt gcaagataaa cattgcccga ctttctggcc
     1261 tctctggcaa tttcatcgat cacctttttc ttgcccctga tgaccatata gttgggagta
     1321 aagtcatcat ccacatcaac acccagccgg ctcttgggaa ggtcgatgat gtggcccttg
     1381 gtggccataa ccttatactt ctttcccaaa aacttcttga gagttttggt cttggtgggg
     1441 gactccacca ccagcaggtc ggtagcagct tttgacccgg tcttagaccc ggtcctcttt
     1501 ttcttcttaa cgcccaatct ttatcttccc gtcgattaat tcctcaatcg cccgcgttgt
     1561 cactttacgg ggatcaatat cgatttcgtc attttccggc attaactcca gcttggcaac
     1621 cctttcggcg ttgatacgcc tggcgcgctt ggccgccact atcacggccg cgtagcgatt
     1681 tttgattacc ttatcaagtt catctaattt gactcgtgcc atcgatcctc ccatgaaaat
     1741 ggctatattt tagtaactac gtttctggat gttttcaatg attccgcttc caatattgac
     1801 tcaatctggc tgacagttgt atccacatct gcgttaacca ccgtgtaatc atatttcgtc
     1861 cgcatctcaa tttcctttaa tgccagggcc agccttttat ccatttcctc tctggaatca
     1921 cgctttctta acttcagtct tcttttaagt tcatttaacg atggtggaag tataaatatt
     1981 gcagctgcat cgggatacga cttctttatc gcacggccgc cctgcacatc aatatcaaac
     2041 aacacaacct tcttttgggc caggtatttt tccacctgtt ccacaggtgt cccataataa
     2101 tgaccatata ccctagccca ttcaacaaac cagccttgtt ccaattttgt ttcaaattct
     2161 tcatttttca tgaaaaaata atctcgcccc tcccgttccc cctttgcctt gggacgggtg
     2221 gtggcagata tcgacattct tatgttgtca tggcgtttga gcaggcccct gcagatagtg
     2281 gtcttgcccg cacccgaggg cgcggagatc accaccagca tcggaccctt gatatatttt
     2341 ttacgcgttg acatctattc cagattctgg acctgctccc tgactttctc gatctcctcc
     2401 ttcatcatga ttacgttcct ggagatttca ggcgagatcg actttgaacc aatggtattg
     2461 gcttctctat aaagttcctg taatataa
//
LOCUS       DSEB01000322            2075 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1009285, whole genome shotgun sequence.
ACCESSION   DSEB01000322 DSEB01000000
VERSION     DSEB01000322.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1009285"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..661
                     /locus_tag="ENO07_05850"
     CDS             <1..661
                     /locus_tag="ENO07_05850"
                     /inference="COORDINATES: protein motif:HMM:PF09684.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01527.1"
                     /translation="NIYRQYDMREGSFLKRFLWLLKHPQIPIDRTIRDIHTYFEPFNT
                     PDKFLPYLASVLALVLDENWDEAKKRELIANIVKIYKLRGTSRGLALYLKIYTESTPE
                     IIENYWPFNGFQVGVSSTIGVDSIIIGYIDRSHCFTVRIPQTIDDTPVEMIRKIHAIV
                     QAEKPAHTNYYMIFAEPELEEADFMQVGVASMIGVESWVGETDNVHEEIEEGKQDDRG
                     E"
     gene            645..>2075
                     /locus_tag="ENO07_05855"
     CDS             645..>2075
                     /locus_tag="ENO07_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01528.1"
                     /translation="MTEESKEYKRVSFFRGFFASEEDFNLLVDYSREKDKLHNQLFHS
                     PGVVLNFSGELKVTAREKGDFSIEVAPGYATDGQGNDIMLWETKVLAIDVSKYKLPMV
                     VYVVLKYYDEPVDFITNKANPQYKGHKRIAERAKVEILPNPPELDEGIELARVRLEEE
                     LKDVKNPADPSHPETGEIDARFVPIAGTFGWILSPEVVSRIFAVYNDMKLVFMQLNKL
                     YDLKYSLEAYQSAITAEMVSIAGKLDYRNAFKLLKIIVDLEKEISNELENTPNLSQRK
                     EVGEYKDNLQALLNLTSATDITSEDLNNLIIYQAKASGALKKLLAPRVAEIRAEVEGE
                     LEEFKGERLSMDEIKIWPKEFPEEIMVDNVRYKLVDKIDILDDASEKEHEFKLGGVKE
                     ELRQKQNFFFPDGTRISDRGRLHWEGFAQFKIKNLKPELDVLVIRRIDYAYGGLKTEI
                     EVDGEKVGVWEITGNDRKYRWRNMPYI"
BASE COUNT          567 a          452 c          561 g          495 t
ORIGIN      
        1 gaatatatac cgtcaatacg atatgcgcga gggctcgttc ctgaagcggt tcctgtggtt
       61 attgaaacat ccgcaaatcc cgatcgaccg tacaatcagg gatatacaca catatttcga
      121 gccgtttaat accccggata aattcctgcc ctatctggca tcggttctgg cgctggttct
      181 cgatgaaaac tgggatgagg ccaagaaacg cgaattgatt gccaatatcg taaagatata
      241 taagctgcgg ggaacctcgc ggggcttggc attatatctg aaaatttata ctgaaagcac
      301 cccggagatt attgagaatt actggccctt taacggtttt caggtggggg tgtcctcgac
      361 cattggagta gattcaatta taatcggcta tatagatcgt tcgcattgct ttacagtcag
      421 gattccgcag acaatcgatg atacgccggt tgagatgatt cgcaaaattc atgcgattgt
      481 acaggcggaa aaacccgcgc atacaaatta ctacatgatc ttcgccgagc ccgagctcga
      541 ggaggccgac ttcatgcagg tcggtgtggc ctccatgatc ggcgtggaat catgggttgg
      601 tgaaacggat aatgttcatg aagaaataga ggaagggaaa caagatgacc gaggagagta
      661 aggaatataa aagggtatca tttttccgcg gcttctttgc ctcggaggag gattttaacc
      721 tgcttgtcga ttattcccgg gagaaggata agctgcacaa tcagctcttt cattcgccgg
      781 gagttgtgct caacttttcg ggcgagctga aagtaaccgc aagggagaag ggcgattttt
      841 cgattgaagt tgcgccgggg tatgctaccg atggacaggg gaacgatatc atgctctggg
      901 aaacgaaggt ccttgctatt gatgtgtcca aatataagct tcccatggtt gtctatgttg
      961 tcctgaaata ctatgatgaa ccggtagatt ttatcaccaa caaggctaat ccgcaataca
     1021 aggggcataa gcgcattgcc gaaagagcca aggtcgaaat tcttcccaat ccgcccgagc
     1081 ttgatgaggg aatcgaactc gccagagttc ggctggagga ggaacttaag gatgtcaaaa
     1141 atccggcaga tccgtcacat cccgaaaccg gtgaaatcga tgcccgcttt gtgcccatag
     1201 ccggtacctt cggatggatt ctctcgcccg aggtggtctc ccgcatcttt gcggtctaca
     1261 atgatatgaa gctggtcttt atgcagctca acaaacttta tgatctaaag tactctctgg
     1321 aggcctacca gtcggcaatt actgccgaga tggtcagtat tgccgggaag ctcgattacc
     1381 gtaacgcctt taaactgctg aaaataatcg tcgatctgga gaaagagatt tccaatgagc
     1441 tcgagaatac ccccaacctc tcacagcgca aagaggtcgg cgaatacaaa gataatcttc
     1501 aggcgcttct gaatctgacc tcggcaaccg atataacctc ggaggatttg aataacctga
     1561 taatctacca ggccaaggcc tccggagcat tgaaaaagct cctggcgccc agggtggcag
     1621 aaatcagggc tgaggtcgag ggcgagctgg aggaatttaa aggcgaacgc ctgtcgatgg
     1681 atgagatcaa aatctggccc aaggaatttc cggaagaaat tatggttgac aatgtgcgct
     1741 ataaattagt tgataagata gatatactgg atgatgcttc cgagaaggaa catgagttca
     1801 agctgggagg agtgaaggaa gagctgaggc agaagcagaa tttcttcttc cctgacggca
     1861 cccggatttc cgaccgcggc cgtctgcact gggagggctt cgcgcagttc aagatcaaga
     1921 atctgaagcc ggagctggat gtcttggtga tacggagaat cgattatgcc tatggcgggc
     1981 ttaagactga gattgaggtc gatggcgaaa aagtgggggt ttgggagatt accggcaatg
     2041 accgcaagta tcgctggcgg aatatgcctt acatc
//
LOCUS       DSEB01000323            3358 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003206, whole genome shotgun sequence.
ACCESSION   DSEB01000323 DSEB01000000
VERSION     DSEB01000323.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003206"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..705
                     /locus_tag="ENO07_05860"
     CDS             <1..705
                     /locus_tag="ENO07_05860"
                     /inference="COORDINATES: protein motif:HMM:PF00534.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HDS01529.1"
                     /translation="DYDLWSPENDNLIPHKYSANDISGKELNKKALLDEAGLRINGHR
                     VPLIGTISRLADQKGFDLIEKIANRLFSLDIAFVLLGTGNENYHILFREMEKIFPERV
                     RAFLKFDNRLAHLIEAGADMFLMPSRYEPCGLNQMYSLKYGTVPVVRKTGGLADTIKD
                     FNMYPLEGNGFVFEEYEADRLLDAIQRAILTYKDQATWKKIMKRGMQADFSWDSSAKK
                     YVRIYEKALEKEKVTI"
     gene            829..2061
                     /locus_tag="ENO07_05865"
     CDS             829..2061
                     /locus_tag="ENO07_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDS01530.1"
                     /translation="MTMDKYYFSTMASRMRRSEIRELLKLTRQPGTISFGGGLPDPNI
                     FPRKAVEEAAVRAIREQGNLALQYSPTEGEPFMKEEAAAFMTRLGDEISAEEIIVVSS
                     SQQALDLLSKVFIDPGDAVIIERPSYVGALQSFRAFGAEFHGVDIDSEGIIPSALEEK
                     ILELRKADKKARFIYLIPDFQNPSGINLSIERRKQVIEIASRHNILIVEDSPYRELRF
                     EGELLPSVRSLDKEGRVIQMKTFSKIFVPGFRIGWLSAPPEVLDKLVMSKQSTDLCTS
                     AFVSMISGYLLKDGHIEKQIEISKEVYVKKCRTMLDALEKYMPELEGLSWSKPEGGMF
                     LWVRLPEYMDTLEMIPDAVEAKVAYVIGSAFYTDGSGKNTMRLNYSYPSHEQIREGIK
                     RLAGIVEKREAARSGAKD"
     gene            complement(2751..2999)
                     /locus_tag="ENO07_05870"
     CDS             complement(2751..2999)
                     /locus_tag="ENO07_05870"
                     /inference="COORDINATES: protein motif:HMM:PF09413.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01531.1"
                     /translation="MTDDGNELVCLRTFNERTQAELTKAYLEAHGIEVIVRADDAGGM
                     IPSITMSASRPCILVRVKDAAEAQRLLKDAEDKLKEQE"
     gene            complement(3193..3264)
                     /locus_tag="ENO07_05875"
     tRNA            complement(3193..3264)
                     /locus_tag="ENO07_05875"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(3230..3232),aa:Cys,seq:gca)
     gene            complement(3272..3347)
                     /locus_tag="ENO07_05880"
     tRNA            complement(3272..3347)
                     /locus_tag="ENO07_05880"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(3312..3314),aa:Gly,seq:gcc)
BASE COUNT          875 a          808 c          821 g          854 t
ORIGIN      
        1 gattatgatc tctggtcacc ggaaaatgat aatctgattc cgcataaata ttccgccaat
       61 gatatctccg gcaaagagct caacaagaaa gccttgctgg atgaggccgg gctgcgcatt
      121 aatggccacc gcgtcccgtt gatcggcaca atctcccgcc tggcggatca aaagggtttt
      181 gacctgatag aaaaaatcgc caaccgcctg ttctcgttgg atattgcttt cgttcttctt
      241 ggtaccggca atgagaatta tcatattctc ttcagagaga tggagaagat attcccggaa
      301 cgtgtgcggg cgtttttgaa attcgataat cgcctggcgc atctgataga ggcgggcgcc
      361 gatatgttcc tgatgccgtc tcgttatgaa ccctgcggcc tgaaccagat gtacagcctg
      421 aaatacggta ctgtgcctgt cgtcaggaag accggcggtc tggcggatac gattaaggac
      481 ttcaatatgt atccgctgga gggaaatggc ttcgtcttcg aggaatatga ggccgacaga
      541 ctgcttgatg ctatccagcg tgcgatcctg acttacaagg atcaggccac ctggaaaaaa
      601 atcatgaaac gcggcatgca ggccgatttc tcctgggatt cctctgcaaa gaaatatgtc
      661 aggatttacg aaaaggctct ggagaaggaa aaagttacga tttgagaaat ctgatcttca
      721 aaggttctct aatatcctcg atctttcctc tcagttttag ttgttttttg ttcacatatt
      781 tttgtaaatt gtgagtaaat ttttgcccgg gtttccacct gaaggagaat gactatggat
      841 aaatactatt tttcgacgat ggcctcgcgc atgcgcagat cggaaatccg tgaactcctg
      901 aagctgacgc gacaacccgg caccatatcc ttcggcggcg ggcttcccga tcccaatata
      961 ttcccacgca aggcggttga ggaagcagcc gtaagagcga ttcgggagca gggcaacctg
     1021 gcattgcagt atagtcccac cgagggagag ccgttcatga aagaagaggc ggccgctttc
     1081 atgacccgcc tgggggatga aatatccgcc gaagaaataa tagtagtgtc ctcctcccag
     1141 caggcgctag acctgttatc aaaggtattt atcgatcccg gcgatgctgt cataattgaa
     1201 cgtcccagct atgtgggcgc cctgcagtcg ttccgtgcat tcggtgcgga attccatggc
     1261 gtggacatag attctgaggg gataatccca tccgcactgg aggaaaaaat cctggaacta
     1321 agaaaggccg ataagaaagc ccggtttatc tatctcattc ccgatttcca gaatccctcg
     1381 ggtataaatc tttctattga aagacgcaag caagtcattg aaattgcctc caggcacaat
     1441 attctgattg tcgaggacag cccctacaga gagcttcgtt ttgaagggga actccttcca
     1501 tcagtaagat cactcgataa ggaaggccgg gttatccaga tgaagacctt ctccaagata
     1561 ttcgtccccg gttttcgcat tggatggctt tccgcgccgc ctgaagtcct ggataagctg
     1621 gtgatgtcca agcagagtac cgatctctgt acctcggctt ttgtttcgat gataagcggc
     1681 tatcttctga aggacggcca tatcgagaag cagattgaaa tcagcaaaga ggtctatgtc
     1741 aagaaatgcc gcactatgct tgatgctctg gaaaaatata tgcccgaact cgagggcctg
     1801 tcatggtcga aaccagaggg cgggatgttt ctgtgggtcc ggctgccgga atatatggat
     1861 acgctggaga tgataccgga tgcggttgag gctaaagtcg cttatgttat cggctcggca
     1921 ttctacactg acggcagcgg gaaaaatacg atgcgtctga attattccta tccgtcacat
     1981 gaacagatca gggagggcat caaacgtttg gccggaattg tggaaaagcg ggaagcagcc
     2041 agatccgggg caaaagattg atccagtgga aaaaaacggc aggccgatag agacctgccg
     2101 cttgcatttg cagtgctaag agctaatatc tttcatcgta ctcttcttcg tcttcctcct
     2161 cgtaatcata gtcatcatca taatcaccat cgtcctcaaa atcgtcgtcc tcatcctcaa
     2221 aattttcgtc atcggcttca tccaaaagct taacagcctc gcgtgagtca tcgggatgga
     2281 ctctcaattc gatccattcg gaagattcgc cgtcatctgt ttcggagtcc cacagctccg
     2341 cgtgaatatc gcatgaaacc agatactccc tggccatctc agcttccgtg cggtcatcaa
     2401 actctcttat tactaccagc ttcttggttt tgttactcat agcaaccttc ttgaatatga
     2461 ggtttaatgt tactaatgtc agtcaatcac ataaaacatg aaaatctatc tatgagcggc
     2521 ttctcctttt acagatgaaa tatcttaacc tgccgactcg gggcagggct tcctgttctc
     2581 tgcagaggcg gcctccggcc tgactgcagc aagcccggca tttgaaagat cggctttatg
     2641 gaaaattaca ttatggctta aaaaagggaa ataaaaatat tctatttttg gaaaaagctg
     2701 cgaggggatt gccatataag acaggaatgt caacaatcga taaaaatcgg ctattcctgc
     2761 tctttcagtt tatcctcggc gtccttgaga aggcgctggg cctcggcggc atccttgacc
     2821 cgcaccagga tgcagggtcg gctggcactc atggtgatag atggaatcat gcctccggca
     2881 tcatcagccc tgactataac ctcgattcca tgggcttcca gatatgcttt cgtcaattcg
     2941 gcctgggtgc gctcgttgaa ggtgcgcagg cagaccagct cgttgccgtc atcggtcatt
     3001 gtcagattcc tttatctaaa gagaaattac agtttttatc caagaaggaa cagcacgaaa
     3061 atgtcattct gaatgagtcg agactttggt cgaagacgac tgaagaatct catggaaatc
     3121 aataagattc ttcgcttcac tcggaatggc gattttactg ttcaggttac caataattcc
     3181 ctgacataaa aaaggcggct tccggattcg aaccggagaa tagaggtttt gcagacctcc
     3241 gccttaccac ttggctaagc cgcccaaaaa atggagcggg aaaccggact cgaaccggcg
     3301 acaatcacgt tggcaacgtg aggctctacc aactgagcta ttcccgcccg aataaatc
//
LOCUS       DSEB01000324            3116 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003801, whole genome shotgun sequence.
ACCESSION   DSEB01000324 DSEB01000000
VERSION     DSEB01000324.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003801"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1200
                     /locus_tag="ENO07_05885"
     CDS             <1..1200
                     /locus_tag="ENO07_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDS01532.1"
                     /translation="PTQFLSTVQIGITAIGILNGIVGEAAIAAPLAELLHGLGLDRNS
                     SSVGATTIVVVSITYLTIVIGELVPKRIAQFNAEGIARLMARPISLLAQLSRPFVFLL
                     SISTDGILRLMGRKELSSANLTEEDIHAILVEGSRAGVIEKYEHDMVRNVFRLDDRQI
                     ASLMTPRNEIVSLDLEQPFEESLDALISSDHSRFPVCRGGMSDILGVITTKRLLRQHL
                     KGEAPASITKYLLPAVYVPESLTGMKLLEQFRESGTQMVFVVDEYGEILGLITLQDFL
                     QALAGEFKPRHPEDVWAVQREDGSWLLDGMIPILELKDRLHLKAVPEEEKGKYHTLSG
                     MMMWLFGKVPRTGDVAEWQGWRLEVVDLDGNRIDKVMASRLPDTGSEGKTEPRAKEDI
                     LNNSIDG"
     gene            1453..1920
                     /locus_tag="ENO07_05890"
     CDS             1453..1920
                     /locus_tag="ENO07_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01533.1"
                     /translation="MVKLKLTVLLFLFSAAAAHGAPIAPKAEPPGKTSDPEVQMQLQS
                     LQDDRIPDERELGIPAYPGARTANVTENSINGLNWIIILSEDPPEKVKEFYENSLSAE
                     WDHWEKFGIFYFSTADRNAAEQHEEPVMEIFKPFGSQAVLMPSAQTIMNIFYR"
     gene            complement(2299..>3116)
                     /locus_tag="ENO07_05895"
     CDS             complement(2299..>3116)
                     /locus_tag="ENO07_05895"
                     /inference="COORDINATES: protein motif:HMM:PF13418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01534.1"
                     /translation="ENIVLPFDPIKRKWPEVTSERVNYSQYSYSYRTIYNTEVPPMNV
                     HRIDHEVVTTSDGKIFVMGGRHEKLKIGIRNEYVGTGEIEVLDSMECYDPTTNKWEFR
                     KPMFNKRFLFAVVVGPDDKIYVFGGGDKYNEDPRSRKVYDTTEVYDPKTDTWTYRAPM
                     PEPRFAHAGALGADGKIYIMGGSLGQDESPPLRGVFIYDPAQDTWEKGPSMLLPRSTL
                     AAVATPDGKIYAIGGTDAGAYKIKKAISLYLPPPPKDYEGRVQETVEMLNITK"
BASE COUNT          792 a          753 c          755 g          816 t
ORIGIN      
        1 cccacccagt tcctctccac cgtgcagata ggcatcaccg caatcgggat cctcaacggt
       61 attgtcggtg aggcggcgat tgccgcccct cttgccgaat tgctccatgg tttgggcctt
      121 gatcggaaca gcagttctgt cggcgcgaca accatcgtgg tggtgagcat cacgtattta
      181 accattgtga taggcgaatt ggtgcccaaa cggatcgccc agttcaatgc cgaagggata
      241 gcgcggctga tggctcgccc gatatcgctg ctggcgcagc tgtcacggcc attcgtcttt
      301 ctgctctcga tatcaacgga tggaatttta cggctgatgg gcagaaagga actgagcagc
      361 gccaatctta cggaagaaga cattcatgcg atcctggtgg agggttcccg ggcgggagtc
      421 atcgaaaaat atgagcatga tatggttcgt aatgtttttc gtctcgatga ccggcagata
      481 gcatctctga tgactccgcg aaacgagatt gtcagtcttg atcttgaaca gccgtttgaa
      541 gaaagcctgg acgcgcttat cagctccgac cactcacgtt ttccggtgtg ccggggaggg
      601 atgagcgata tattgggagt catcaccaca aaacgtctgc tcaggcagca tttaaaggga
      661 gaagccccgg cctccatcac caagtatctg ctgcccgcgg tttacgtgcc tgagtcgctg
      721 acgggaatga aattgctgga gcagtttcgt gagtcgggaa cgcagatggt ttttgtcgtt
      781 gatgagtatg gcgaaattct cggcctgatc actctacagg atttcctgca ggcgctggca
      841 ggggaattca agccgcgcca cccggaagat gtgtgggccg tgcaacgtga ggacggctcc
      901 tggctgctgg acggcatgat tccaatcctg gagctgaagg atcggcttca cctgaaagcg
      961 gtccccgagg aagaaaaagg aaaataccat accctcagcg gcatgatgat gtggctcttt
     1021 ggcaaggttc cgcgcacagg cgatgttgcc gaatggcaag gttggcggtt ggaagtggtc
     1081 gatctcgacg gcaaccgaat cgacaaagtg atggccagtc gtttgcctga caccggcagc
     1141 gaagggaaaa cggagccacg tgcaaaggag gacattttaa ataattcaat tgacggttaa
     1201 caagttgatc gagatggcga attacttaat ttcttaggat gtcccttgga ctggtgctgg
     1261 actggtgccc ctggaacttc gtagaattct ctgaccagat ttgacaagag gggcagaaaa
     1321 agcgcaccgt aacctaaaag aataccttaa aagcaacgaa tcctccctgc atatttgatt
     1381 ccggcttcat gcgatgatag tattcaacaa aaaattgaaa cgatcccgtt aaatcaaccc
     1441 aaggagaaaa cgatggtaaa actgaaattg acggtgcttc tttttctttt ttccgctgcc
     1501 gccgctcatg gggctcccat agctcccaag gcagagcccc ccggcaaaac ctcagatccg
     1561 gaggttcaga tgcagctgca gtctttgcag gatgatcgaa ttcccgatga aagagaattg
     1621 ggaataccgg cctatccggg agcccggacc gccaatgtta cggaaaactc aatcaacggg
     1681 cttaactgga taatcatttt gagtgaagat ccgcccgaaa aggtgaaaga attttatgaa
     1741 aacagccttt ctgccgaatg ggaccattgg gagaagttcg gtatatttta tttttccact
     1801 gctgatcgca atgccgcaga gcagcatgaa gaaccggtga tggaaatctt caaacccttt
     1861 ggcagtcagg ctgtgctgat gccttccgcc caaaccataa tgaacatttt ctatcgatag
     1921 gatcggtctc ggattgccgc tatattggga aattactatg gagccgatga gcggacttga
     1981 accgctgacc tactgattac gaattgattt cctccgagct cacacaacct ccggcactcg
     2041 gcaacatgat gaaattatta ggtttaaatt ttacacattt tttccgattt tatacaaaac
     2101 agcttatttc ggaatgtttt tctcacacga atccacacac gggaaatggg atttttcggg
     2161 ataattcttg aattcggcaa ttcgctcctt cgatcctgtc cggcattttt cgctccagcg
     2221 aggatgtcgc ttaaaaagca aaatgggtca tttattaaaa aacccatttt cttctgtaaa
     2281 acacctaaaa ctttcaattc atttggtaat atttaacatc tcaacagttt cctggactct
     2341 cccctcataa tctttagggg gtggcgggag ataaagacta atagcctttt ttattttata
     2401 tgcccccgca tctgtaccgc cgatggcata tattttcccg tcaggagtag caactgctgc
     2461 cagtgtcgag cgggggagca acattgacgg ccctttttcc caagtgtcct gagcagggtc
     2521 ataaataaac acacctctca aaggtggact ctcatcctgc cccaggctgc cccccattat
     2581 gtatatcttt ccatcggcac cgagagcccc ggcatgggcg aatctcggct caggcatagg
     2641 agcccggtat gtccaggtat cagtcttcgg atcatagact tccgttgtat cgtagacttt
     2701 tctggacctg ggatcttcat tatacttgtc gccaccgccg aaaacataga ttttatcgtc
     2761 cggtccaacc acaactgcaa aaagaaaccg cttattgaac attggttttc tgaattccca
     2821 cttgttggtt gtgggatcat aacattccat tgaatccaga acttctattt cgccagttcc
     2881 aacgtattcg tttctaattc caattttaag tttttcgtgg cgaccaccca tcacgaatat
     2941 cttgccgtcc gaggtggtaa caacttcatg atctattcta tggacgttca taggtggaac
     3001 ttctgtatta tagatagttc tatagctata agaatattgc gaataattta cccgttcgct
     3061 tgtaacttcc ggccattttc tttttatggg atcaaaaggc aatacaatat tttcac
//
LOCUS       DSEB01000325            2895 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004448, whole genome shotgun sequence.
ACCESSION   DSEB01000325 DSEB01000000
VERSION     DSEB01000325.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004448"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1622
                     /gene="recJ"
                     /locus_tag="ENO07_05900"
     CDS             <1..1622
                     /gene="recJ"
                     /locus_tag="ENO07_05900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDS01535.1"
                     /translation="NILKILINRGLDTAEKVNHFINPSMSDLKDPFLLPDMEKGVERI
                     VEALRENEKMMVYGDYDVDGITAAGLMFLVLNKLGAQISYYLPNRLVEGYGISEDGLA
                     EAVKQGVTLIISVDTGVTAVDEVERARELGIDIIVTDHHEPGPNLPKAVALINPKLHD
                     EDHGSHELSGVGVAFKVAQALYRRLNQPESELEEHLDLVALGTSADIVPLVDENRILT
                     KFGIKQIIRTTKPGLKSLTFVSGLLGKEISTGQVVYVLAPRINAIGRLGNAEKAIRLL
                     TTRDEAVASKIAKFLEEENRRRKSIDEKTLTEALEMIETEVDLKNDRAIVLASKGWHQ
                     GVIGIVASRLVEKFYLPTILIAIDGDEGKGSARSIPNFHIYNALNFCSDDLLRFGGHK
                     YAAGLTIKPSKIEIFSRKFKQVALEELTDEDLTPKLKIDAEIDLDDIDEKFIDTIDMF
                     APFGPANMRPIFLTRNLEAVGTPHVVGKNHLKMKVKKGEKVFDVIGFGFGEHAHQLAL
                     RSVAVDLVYVAEMNYFGDQSKIQLRIKDLRY"
     gene            1625..2569
                     /gene="meaB"
                     /locus_tag="ENO07_05905"
     CDS             1625..2569
                     /gene="meaB"
                     /locus_tag="ENO07_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014122484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HDS01536.1"
                     /translation="MSLLDRFKKREPRALSRIISYVENREENYRKVLSTLYKDTGKAY
                     KIGLTGPPGAGKSTLMDSITEILVNDGNRVGIIAVDPSSPFTGGAFLGDRIRMQNLSS
                     NENVFIRSMATRGSMGGLARATKDVSMVYDAYGFDYILIETVGVGQVELDIVDAADTV
                     VVAIVPESGDVIQAMKAGLMEIANVFCVNKADREGSDRVISELNHLLQLKRERADWGY
                     PVIPTSAVKKEGIDLLIKAIMQHKDYLMESGRFKANRKEQIRKDIIEHIRTMLSANIE
                     DQLHSAETFENLLEDIYNRKNDPVKTAMELYDRHYSKK"
     gene            2685..>2895
                     /locus_tag="ENO07_05910"
     CDS             2685..>2895
                     /locus_tag="ENO07_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HDS01537.1"
                     /translation="MFDKETIKKIKEAKAKWKAEAEKTENKDKKFVTISSEPVDFMST
                     PDRIDDMDYFEDINFPGEYPYTRGVH"
BASE COUNT          797 a          662 c          766 g          670 t
ORIGIN      
        1 ataatattct taaaatcctt attaaccggg ggcttgatac tgcagaaaaa gtcaatcatt
       61 tcattaaccc atctatgtcg gatctcaagg atccgttttt gctgccggat atggaaaaag
      121 gggtggagcg catcgttgag gcgctgaggg aaaatgaaaa gatgatggtt tacggggact
      181 atgatgtcga tggcatcact gccgcgggat tgatgtttct cgtgctcaac aagctgggag
      241 cccagatcag ctattatctt ccaaaccgtc tggttgaggg atatggtatt tctgaggatg
      301 gcctggctga agctgtcaag cagggggtga ccctgattat ctctgtcgat acgggtgtaa
      361 ctgctgtcga tgaggtggag agggcacgcg aactgggtat cgatataatc gtgaccgacc
      421 atcatgaacc cggtccgaat ctgcctaaag cggtcgcgct catcaatccc aagcttcacg
      481 atgaggatca tggcagccat gagctttcgg gcgtgggcgt ggccttcaag gttgcccagg
      541 ccctttacag acggctcaat cagcctgaga gcgaactgga ggaacatctg gacctggttg
      601 ccctggggac atcagccgat atagtaccgc ttgtggacga gaaccgtatc ctgaccaaat
      661 tcggtataaa acagatcatc cgcaccacca agccgggcct gaagtctttg acctttgttt
      721 ccggtctgct gggcaaggag atatccaccg gacaggtggt ctatgtgctg gctccccgta
      781 ttaacgccat cgggcggctg gggaatgccg aaaaggctat caggcttctg accaccaggg
      841 acgaggcggt ggcctcgaaa attgccaagt tcctggagga agaaaacaga cgccgtaaat
      901 cgattgacga gaagaccctg actgaagctt tggaaatgat cgaaacggag gtcgacctga
      961 aaaatgaccg cgcaattgtg ctggcctcca agggatggca tcagggcgtc attggaatcg
     1021 tggcctcacg cctggtcgaa aagttctacc tgcctaccat attaatcgca atcgatggcg
     1081 atgagggcaa gggttctgcc aggtcgattc caaatttcca tatttacaat gcactgaact
     1141 tctgttccga tgatctgctg cgcttcggtg ggcataaata tgcggcgggc ctgacaataa
     1201 aacccagcaa gatcgagatt ttctcacgca aattcaaaca ggtcgccctg gaagaattga
     1261 ccgacgagga cctcacacca aaacttaaaa ttgatgctga aattgatctt gacgatatcg
     1321 atgagaagtt tatcgataca atcgacatgt ttgccccgtt cggtccggct aatatgcgcc
     1381 ccatcttcct gaccagaaat ctcgaggccg taggcacgcc tcatgtagtc ggaaagaatc
     1441 acctcaagat gaaagtgaaa aagggcgaga aggtttttga tgttatcgga ttcggtttcg
     1501 gcgaacatgc ccatcagctt gccctccgtt ctgtggcggt tgacctggtc tatgtggccg
     1561 aaatgaacta tttcggcgat caatcgaaga ttcagcttcg cattaaggac ttgagatatt
     1621 agctatgtcg ctgcttgaca ggttcaagaa aagggagccg agggcgctat caagaatcat
     1681 cagctatgtc gaaaaccgcg aggagaatta ccgcaaggtt ctctccactc tctacaaaga
     1741 taccggaaaa gcttataaaa tagggctgac cgggccaccc ggggccggca agagcacact
     1801 catggactcc ataacggaaa ttcttgtcaa tgacggcaac cgcgtcggca taatcgcagt
     1861 tgatccatcc tcgcccttca ccggcggagc gttcctgggc gatcgaatcc gcatgcaaaa
     1921 tttaagcagc aacgaaaatg ttttcatccg cagtatggca acgcggggtt ctatgggcgg
     1981 tctggcccgc gccacaaaag acgtcagcat ggtttatgat gcatatggtt tcgactatat
     2041 attgatcgag actgtaggag tggggcaggt ggagctcgat atcgtcgatg ccgctgatac
     2101 agtcgtggtt gcgattgtgc cggaatcggg ggatgtgatc caggctatga aggcggggct
     2161 gatggagatt gccaatgtct tctgcgtaaa taaggcggat cgcgaaggct cggatcgggt
     2221 gatatcggaa ttgaatcacc tgctgcaact taagcgtgag agggctgact ggggctaccc
     2281 ggtcataccg acctcggcgg tcaaaaaaga aggcattgac ctgcttataa aggcgatcat
     2341 gcagcataag gattacttga tggaatccgg tcgattcaag gccaaccgca aagagcagat
     2401 caggaaagat atcatagaac atatccgcac catgctttcg gccaacattg aggatcaact
     2461 gcattcagcc gaaacattcg agaacctctt ggaggatata tacaaccgta aaaacgaccc
     2521 ggtcaaaacc gctatggaac tttacgacag gcattactct aaaaagtaga acaatactcc
     2581 attcccagtg ttaattatta cttgaaataa gcgccacaaa agcttatttt taatgtttat
     2641 attgcgatga aaaatatcta atcctctaac atctcgaggt gagtatgttc gataaagaga
     2701 ctattaaaaa aataaaagag gccaaggcca aatggaaagc cgaggccgaa aagacggaaa
     2761 ataaagataa gaaattcgtg accatatcct ccgagccggt agattttatg agtacgccgg
     2821 atcgaattga cgatatggat tacttcgagg atataaattt tcccggcgaa tatccgtata
     2881 cgcgcggggt gcatg
//
LOCUS       DSEB01000326            5351 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001125, whole genome shotgun sequence.
ACCESSION   DSEB01000326 DSEB01000000
VERSION     DSEB01000326.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5351)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5351)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5351
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001125"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..698
                     /gene="prs"
                     /locus_tag="ENO07_05915"
     CDS             <1..698
                     /gene="prs"
                     /locus_tag="ENO07_05915"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDS01538.1"
                     /translation="DSVTLIMPYFSYAKGDKKDEPRVSIRARVCADAIEAAGADRVVA
                     MDLHAPQIQGFFRVPVDNLYALPVLCDRIMELKLKNIVVVSPDTGFANQARRYASRLD
                     ASVAIGDKRRTAHDEKAEILEVIGDVEGKTAIVVDDFTISGGTLVDVSRKLEEMGAAE
                     IYAMVTHGVFGEGSMAKIDDSPIKKLLVTDTVENQPEEFSPKIEIVSAAPLFGEAIKR
                     IHDRDSISSMFTD"
     gene            complement(825..2045)
                     /gene="trpB"
                     /locus_tag="ENO07_05920"
     CDS             complement(825..2045)
                     /gene="trpB"
                     /locus_tag="ENO07_05920"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_800095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDS01539.1"
                     /translation="MQVRKRIENQPDNKGYFGEYGGRYVPEILIEILDEITTAYLNLK
                     DQKGFKEELSYLYRHYVGRPSPVFYAQNLSARLGGAKIYLKREDLNHTGAHKINHCLG
                     EALIAKKMGKKKIIAETGAGQHGVALATAAAIVGLECEIYMGEVDMAKEAPNVTRMRI
                     LGTNVVPVGFGLKTLKEAVDAAFEAYMKDPVDQFYAIGSVVGPHPFPMMVRDFQQVVG
                     EEAMVQFMELTGRLPDNLVACVGGGSNAIGLFSAFLDEDRVEMYGVEPAGLSFKKGEH
                     AATLTLGSPGMIHGFKCYLLQDDQGRPLPVYSIASGLDYPGVGPQHSYLKDIGRVQYK
                     TINDKEALDAFFTLSREEGIIPALESAHAVAYAIKMAPDLDQDKTILVNLSGRGDKDI
                     EYVVEKYGKKYGIA"
     gene            complement(2008..2319)
                     /locus_tag="ENO07_05925"
     CDS             complement(2008..2319)
                     /locus_tag="ENO07_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDS01540.1"
                     /translation="MRSVNKVIKIFSEINDPTVMRKLFSEIFTPAEIQDVALRWRLME
                     MLDQGIPQRKIASDLGISLCKITRGSKILKKRNSVSKRLLNQTTGGRNAGKKTNRKPA
                     G"
     gene            2614..4308
                     /locus_tag="ENO07_05930"
     CDS             2614..4308
                     /locus_tag="ENO07_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HDS01541.1"
                     /translation="MNKNDNEKPGKPSNFIREIIDRDLEQGKNNSRVHTRFPPEPNGW
                     LHIGHAKSICLNYGLAQDYNGKFNLRFDDTNPLKEESSYVESIIKDVEWLGAKWEDRL
                     FYASDYFEQLYEWAIKLIKEGKAYVDDLNAEEIREYRGTLTEPGKNSPFRDRSIEENL
                     KLFEKMKNGDFPDGSKVLRAKIDMASGNINLRDPVMYRIKHAAHHRTGDKWCVYPSYD
                     WAHGQSDSIEGITHSICTLEFEDHRPLYDWFLDQLEVHHPRQIEFARLNLNYTIMSKR
                     KLLQLVQEKYVSGWDDPRMPTISGLRRRGYSPESIREFCERIGVAKRESVVDIALLEH
                     TLREDLNKRAVRMMAVLRPLKVVIENYPEDKVEEFEAVNNPEDESAGTRTIVFTREIY
                     IERDDFLEDPPRKFFRLAPGREVRLKHAYFITCNEAVKNDAGEITELRCSYDPESRGG
                     STPDGRRVKGTLHWVSAEHAVDAEVRLFDRLFDKEDPTEGGDFLKNINPDSLEILTGC
                     KVEPGLKDAKSGEYYQFLRQGYFVADSEDSTPDHPVFNRSVGLRDTWARIQQKGAK"
     gene            4421..5167
                     /locus_tag="ENO07_05935"
     CDS             4421..5167
                     /locus_tag="ENO07_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDS01542.1"
                     /translation="MPVHEFDIITLDQKPNLKHEIDKLSELSWPKFLLHGDVRYWSSL
                     FNVFAQYQIAFVLDDDRVIAAGHTVPLRWDGSPDDLPSSLDALMHRAFRDRQVKSKPD
                     SISALAIMIHPDFRNEKLSARMIKAMIWLGKKYGMGNLLAPLRPTLKSNYPSMPFDEY
                     IDKKAENGETFDPWIRIHLRLGGEIIKIMDKSITVTGTLSEWEEWTDMKFPESGDYTI
                     KGGMQPLVIDEAKNVGIYTEPNLWVRHRIR"
BASE COUNT         1376 a         1235 c         1364 g         1376 t
ORIGIN      
        1 ccgattctgt gaccctaata atgccgtatt tcagctatgc caagggggac aaaaaagatg
       61 agccgcgggt atcgatacgc gcccgtgtat gcgccgatgc tatcgaggct gccggcgcag
      121 accgcgttgt ggcgatggat cttcatgccc cgcagattca gggatttttc cgtgttccgg
      181 tggataatct ctatgccctg cctgttttat gcgaccgaat catggaactg aagctcaaga
      241 acattgtggt tgtctctccc gataccggct ttgccaatca ggcccgcagg tatgcatcac
      301 ggctcgatgc ctcagtcgcc atcggggata aacgccgcac cgcacatgat gagaaggccg
      361 agattctgga agttatcggc gatgtggaag gtaagactgc gattgtggtt gacgatttta
      421 cgatttcggg cggcacgctt gttgatgtat cgcgcaaact ggaggagatg ggcgcggcag
      481 agatttatgc catggtgact catggagtct ttggagaggg atcgatggcc aagatagatg
      541 acagccctat caagaagctg cttgttacag acaccgtgga aaatcagccg gaagaatttt
      601 ctcccaagat tgagattgtc tctgcggcgc ctctattcgg ggaagccata aagcgcatcc
      661 atgaccgcga cagcatcagt tcgatgttca cggactaaaa tttaagccgg aatttataga
      721 cagagagatt gaaaccccga cccaatccac acgctaatta aaaccggaca gcgtatcaaa
      781 ccgctgcccg gtcgcatcaa agaagtattg agtcttcaca ttcctcatgc tatcccatat
      841 ttcttgccat atttttcaac cacatattct atatccttgt cccctcttcc ggagaggttc
      901 acgaggatgg tcttatcctg atccagatcg ggagccatct tgatggcata agcaacggca
      961 tgcgcgcttt caagagccgg aatgatcccc tcttccctcg aaagcgtaaa gaaggcatcc
     1021 agcgcctctt tatcattaat ggttttatac tgcacccgcc cgatgtcttt caggtagctg
     1081 tgctgggggc cgactccggg gtaatcgagg ccggaagcaa ttgaatagac cgggagtgga
     1141 cgcccctggt catcctgcag aagatagcat ttaaagccat gaatcatccc cggagaaccc
     1201 agggtcaggg tcgcggcgtg ttcacctttc ttgaagctaa gccctgctgg ttcaacgcca
     1261 tacatctcga cccggtcctc gtcgaggaag gccgagaaaa gtccaatggc gtttgatccg
     1321 ccgcccacgc atgccaccag attatcgggc agcctgccgg tcagctccat gaactgaacc
     1381 atagcctcct cgcccacaac ctgctggaaa tcgcgcacca tcattggaaa tggatgcggg
     1441 cccactactg agccgatggc atagaactgg tcgaccggat ccttcatata agcctcaaag
     1501 gcggcatcaa cagcttcttt aagcgtcttc aggccgaatc cgaccggcac gacattggtg
     1561 ccaagaatac gcatacgggt gacattgggc gcctccttgg ccatatccac ctcgcccata
     1621 tagatttcgc attccagccc gacaattgct gcggcggttg ccaatgcgac gccatgttgt
     1681 cccgctccgg tttcggcgat tatcttcttc ttgcccatct ttttggcaat cagcgcctcg
     1741 cccaggcagt ggttgatttt gtgggcgccg gtatggttta aatcttcgcg ctttagatag
     1801 attttggcgc cgcccagtct ggctgagaga ttttgagcat agaaaaccgg actggggcgt
     1861 ccgacataat gccggtataa ataggagagc tcttccttga aacctttctg gtcctttaag
     1921 ttaagatatg ctgtggtgat ttcatccaga atttcaataa gtatttcggg gacatatcgt
     1981 ccgccgtatt ctccaaaata acctttatta tccggctggt tttcgattcg ttttcttacc
     2041 tgcatttctt cctcctgttg tttgattcaa gagtctcttc gatacagaat ttcgcttttt
     2101 gagaattttt gagccgcgcg ttatcttgca caggctgata ccgagatccg aagcgatttt
     2161 acgctggggg ataccctggt cgagcatctc catcagacgc catcgaagag cgacatcctg
     2221 aatctcagca ggagtaaaaa tctcgctgaa gagtttccgc atgactgtcg gatcattaat
     2281 ttcgctgaat attttgatta ctttgttgac tgatcgcata gctcatctac aagtgtttct
     2341 atttatagaa acattatata ttaaatgccg attttgtcaa gccctttttg tgggcttttc
     2401 gagagaattt ttgtcatcct gcccacgatt tgagatttgc tgatttatgt tgtggagcgg
     2461 ccgatttttt atatatttag gctttccaaa attggaagac tggagagatt tttgttttga
     2521 acgaaaaaag acattaatta ttttccggat tgttcgaatt gtatgatatg agaatgctca
     2581 aaggtggata ttaatattat ggaagacaat aagataaata agaatgataa cgagaagccg
     2641 ggaaagccgt cgaatttcat aagggagatt atcgacaggg acctggagca gggcaaaaat
     2701 aacagccgcg tacatacccg ttttccgccc gagcccaacg gctggctgca tatcgggcat
     2761 gccaagtcga tttgcctgaa ctatggcctg gcgcaagatt ataacggcaa attcaatctc
     2821 aggttcgatg atacaaatcc gttgaaagag gaatcctcct atgtggagtc gatcataaag
     2881 gatgttgaat ggctgggggc aaagtgggag gaccgccttt tttatgcctc ggactacttc
     2941 gagcagctct atgagtgggc catcaagctt atcaaagagg gcaaggcgta tgttgatgat
     3001 ctgaatgccg aggaaatcag ggaatatcgc ggtaccctga cagagccggg aaaaaatagt
     3061 cccttccggg atcgttcgat tgaagaaaat ctgaaattat ttgagaagat gaagaatggc
     3121 gattttccgg atggttccaa ggttcttcgg gcaaaaatcg atatggcatc cggcaatatt
     3181 aatcttcgtg atccggttat gtaccgtatc aagcatgccg cgcatcatcg tacgggagat
     3241 aaatggtgcg tgtatcccag ctatgactgg gcgcatggcc agtcggactc gatcgagggc
     3301 ataacccact cgatctgcac actcgaattc gaagatcatc gcccgctcta cgactggttt
     3361 ttggatcagc tggaggtaca tcatccccgt cagatagaat ttgcgcgtct gaacttgaat
     3421 tatacgatta tgagcaaacg gaagcttctg cagctggtgc aggagaaata tgtgagcggc
     3481 tgggatgatc cgcgcatgcc cacaatttcc ggtctcaggc gccggggcta ctcgcccgag
     3541 tcaatccgtg aattctgcga gagaatcgga gtggccaagc gcgagagcgt ggtcgacatt
     3601 gcgcttctgg agcataccct gcgcgaggac ctgaataaac gcgcagtccg aatgatggcg
     3661 gtgcttcggc cgctgaaagt tgtaatcgag aattaccccg aggataaggt agaggagttc
     3721 gaggctgtca ataatcctga ggatgaatcc gccggaacca ggactattgt attcacgcgt
     3781 gaaatatata tagagcggga tgattttctg gaggatccgc ccaggaagtt tttccgcctg
     3841 gcgccgggac gcgaggtgcg cctgaaacat gcctacttca ttacctgcaa tgaggctgtt
     3901 aagaacgatg cgggggagat tacggaattg cgctgcagtt atgatccgga atcccgggga
     3961 ggaagcactc ccgatggaag aagagtgaag ggcacgttgc actgggtttc tgccgaacat
     4021 gctgttgatg ccgaagtcag attgttcgac cgtctattcg acaaagagga tccaaccgaa
     4081 ggcggcgact ttttgaagaa tatcaatccg gactcgctgg aaatactgac tggctgcaag
     4141 gtcgagccgg gattgaaaga tgcaaaatcg ggggaatact accaattttt gcgacaggga
     4201 tacttcgttg cggattccga ggattcgact cctgatcatc cggtttttaa ccgttctgtc
     4261 ggtctgcggg atacctgggc aaggattcag caaaaaggcg cgaagtaact tacgatctga
     4321 ataatagagg cttaaaaagt ttagttcccg tccctttgaa gaagggatga gaactgacag
     4381 tatgtgaaat ttctttcgct cagtccaagt agagctgcag atgccggttc atgaatttga
     4441 catcatcact ctggatcaaa aacccaattt gaagcacgag attgacaaac tcagtgaatt
     4501 gtcgtggccg aagtttttgc tgcatgggga tgtgcgctac tggagttcat tattcaatgt
     4561 attcgcccaa tatcagatcg cttttgtttt ggatgacgat agagtaattg ccgccgggca
     4621 tacagtaccc ctcaggtggg atggctcgcc tgatgatctt ccctcgagcc tcgatgcact
     4681 tatgcatcga gcattccggg atcgtcaagt caaaagtaaa cccgacagca tttcagctct
     4741 ggctataatg attcaccccg atttcagaaa tgagaagtta agtgccagga tgattaaggc
     4801 catgatatgg cttggaaaaa aatatggtat ggggaatctg cttgctcctc tccgacccac
     4861 attgaagagc aactaccctt caatgccctt tgatgaatat atcgataaaa aggccgaaaa
     4921 tggagaaaca ttcgatccct ggatccgcat acatttgaga ctgggtggcg agataattaa
     4981 aattatggac aaatctataa ccgttacggg tactttatct gaatgggagg aatggacgga
     5041 catgaaattt cccgaaagcg gtgattacac cataaaaggg ggcatgcagc cccttgtaat
     5101 tgatgaggcg aaaaacgtcg gtatttatac cgagcccaat ctgtgggtgc gtcacagaat
     5161 aaggtaaaca tcctatcttt tttcccccat tatacttgca ataatgcctc cgatgtttta
     5221 taatcagaga taatagaacc gacttattcg tgctgtcaag tgccaacgta agttttaaaa
     5281 gcgggtatta tctggaacac tttgagaccg ggttctgttt ttgaattcaa agaggagcac
     5341 cagtttatac c
//
LOCUS       DSEB01000327            5355 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001122, whole genome shotgun sequence.
ACCESSION   DSEB01000327 DSEB01000000
VERSION     DSEB01000327.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001122"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..608)
                     /locus_tag="ENO07_05940"
     CDS             complement(<1..608)
                     /locus_tag="ENO07_05940"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:PF03853.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HDS01543.1"
                     /translation="MKIVTAEQMQDIDKAAIEGRGIPGLELMENAGRGIAEYLYEISD
                     DDVTAKSVAVICGKGNNGGDGFVVGRYLAEWGADVRFYLLGKKDDLKGDAATNLKRAQ
                     EMDLPIHEISSAEELDLDEETDLIVDAIFGTGFHGEIRSPMDKIVDKINSCDIPIAAV
                     DCPSGLDVSTGEVSRSAIMANFTATMALPKIGQFLHPGVEYVG"
     gene            855..1253
                     /locus_tag="ENO07_05945"
     CDS             855..1253
                     /locus_tag="ENO07_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01544.1"
                     /translation="MAGFEKAVYEFYAAIDDGRDSARAEMFTEDALMLPNHWDYTRGK
                     EAITRSITGSQGWVFKLKDLKHLDMGVSADLGYTVNEYYYTWHPEGQEPQWHKTKNIH
                     IWKKQPDGIWKLHADLWNSSEPLARPGSKE"
     gene            1432..2298
                     /locus_tag="ENO07_05950"
     CDS             1432..2298
                     /locus_tag="ENO07_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01545.1"
                     /translation="MNRALVFVMVLAVGVVFLIAQGQHNESEIASEASAMLPYALDSL
                     YPPVAKSPVYLEEMVSLSNSFAAIMIDFQANDNANAMKHFEEFKAKYEKCSELVPEWK
                     NKYSTEHVNALGEALKSGTPEAVMRSYELVGANCHSCHLQYMSDVQYKYHWPDFADFI
                     ITDPILKKDLSYARFMMFIVTSMIGIEVDLQEGQIENARNHLSRFASRFETLTESCEL
                     CHADQRHHFVDNSMQEVLLSLQEAFEAEPIDIQLLRTSLQTLGTESCYKCHLVHIPPT
                     YAKARWRPAGPH"
     gene            2488..4374
                     /locus_tag="ENO07_05955"
     CDS             2488..4374
                     /locus_tag="ENO07_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01546.1"
                     /translation="MQTTRLVLLIAVLMLFIHVIALSEVPNMFNYQGRLLDDAGNPLN
                     GTVRLDFGIYADTGGTTQLWVETHTSVPVTDGLFKVQLGSATTLPNSVFDGSLRYLGI
                     RVNSGTMLRPLTPIVASPYSFRALHADTAAYAMSAPGGVDGWSQSGNYVYLADIGDSV
                     GIGTISPGSPLEVQGARTAIVGQSTGSGIMAGAITGLNTGSGHGVMGISSDGPGVYGL
                     STNGPGVHGFSSSGFGGYFDAPKHYFTANVGIGTTEPEYPLDVHSSAVSVRGYTTGGP
                     GTSGIYGRAENNAYGVMGTSSSGRGVYGASTTGFGGYFMGPKHYLNGLTGFATEEPTH
                     RVTVNGAIGIQQSGTTKYHLNYYNNGFNISETNVADYRLYIKEGGNVGIGTSNPTQKL
                     YVNGNAHVAGTFHADAFEANAIGKENIIDEVGLAHASNSGSSSLTTSYAAYLTKQITV
                     PTAGYILAIGCAHFMFDHGVSGSSGGYLAFSDLPDNINGGINQLFMLGHSVSAGYYRV
                     SIPCQRLFYVSGAGTYSFYVIAKREADNSASIAAPQMALLFIPTAYGSKDGVLSIDPE
                     DDIYTFSGRQELEEKLTGASQLEEPVAGSSAEGDNGALIKQVKSLTAEIEVLKSRMEA
                     YEGH"
     gene            4623..>5355
                     /locus_tag="ENO07_05960"
     CDS             4623..>5355
                     /locus_tag="ENO07_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01547.1"
                     /translation="MKAAKVFLTIAVFVFVVFVSLIADVPHFFNYQGRLMDDAGNPLN
                     ATVELDFGIYTGESGTGLLWQEVHPSVSVVDGLFNVYLGTGNMLPDSLFDGSVLWLGI
                     KVNDGPIMQPLTPFVASPYSFRAIHADTAAYALDAPAGSSVWSQSGNYVRLPDVGDSV
                     GIGVLNPVEKLHVGGNILLGSYGDIRFSPDNTRINCESNDLQIKAQTDLYLEPYDDLY
                     IRRRDGGSSWAHFDNSTQRLGIGVLD"
BASE COUNT         1330 a         1256 c         1359 g         1410 t
ORIGIN      
        1 cccacatatt caacgcccgg gtgcaggaat tgtcctatct tgggtagagc catggtggcg
       61 gtgaagttgg ccatgatggc acttcgagaa acctcgccgg tagagacatc cagcccgctg
      121 ggacaatcga ccgcggctat cgggatatca caggaattta tcttatcgac tatcttatcc
      181 atcggagacc gaatttcgcc atgaaacccg gttccgaaaa tggcatcgac tatcaggtcg
      241 gtctcctcat ccagatcgag ttcttcggca gaagagattt catgtatcgg cagatccatt
      301 tcttgagcgc gcttaaggtt ggttgcggcg tcgcccttga ggtcgtcctt cttgccgagc
      361 agatagaacc tgacatccgc gccccattcc gcaagataac gtcccacgac aaaaccgtca
      421 ccgccattat ttcctttacc gcagattacc gccacactct tggcagtaac atcgtcatca
      481 gagatttcat aaagatactc agcaataccg cgcccggcgt tctccatcaa ttccaggccg
      541 gggatgccgc gtccctcgat ggcggccttg tcgatatcct gcatctgttc tgcggttact
      601 atcttcatgc tgttcagaat ataaaaataa gctgtacttg tcaatttgat atttgatgtt
      661 gtttattctt gaccgtcatt tcagtgcagt tatttttaga aagacttggg tgccccgcca
      721 aagtgaatta ataaatctca cgcattaact ttcgggagtg cattatgaat gccaggacgc
      781 tatttgcagc agttttcgca gcaataacac tgttatgttt atcctgttca tctgaacaac
      841 agcagacatc ggacatggcc ggttttgaaa aggcagtcta cgaattctat gccgccatcg
      901 atgatggcag ggactcggca cgggcggaga tgtttactga ggatgctctc atgctcccca
      961 atcattggga ttacacgcgc ggcaaggaag ccattacccg cagtataacc ggcagtcagg
     1021 gctgggtatt caaacttaaa gatctgaagc atctggatat gggagtgagc gcagatctgg
     1081 gctatactgt caatgaatat tattatacct ggcatccgga gggccaggaa ccgcaatggc
     1141 acaaaaccaa aaacattcat atctggaaga agcagcctga cggtatctgg aaactgcatg
     1201 ccgacctctg gaattcaagc gagcccctcg ctcggcccgg gagcaaagaa tagcctttat
     1261 tcagcccagt tccttaagcg gtctgtaccg cttgtggaaa gcggattaaa ttagtcagca
     1321 attggcatgc ctggattgaa agaaaacttt tattgtcctg aaatattttt cttgtttagc
     1381 attccgataa ggattaattt tgattatgca aattttaaaa aggaggaatt agtgaacaga
     1441 gcgctcgtct ttgttatggt actcgctgtt ggagttgtgt ttcttattgc ccagggccag
     1501 cacaatgaaa gcgaaatcgc ttctgaggca tcagccatgc tgccttatgc actggacagc
     1561 ctttacccgc cagtagcaaa atccccggtt tatctggagg agatggtcag cctgtcaaat
     1621 tcgtttgcag ctatcatgat tgattttcag gccaatgaca acgccaatgc catgaaacac
     1681 tttgaggagt tcaaggccaa atatgagaag tgttcagagc ttgtgccaga atggaaaaac
     1741 aagtactcaa cggagcatgt caatgctctt ggtgaggcac ttaaatcagg cactccggag
     1801 gccgtcatga ggtcctacga gctggttggt gcaaactgcc acagctgcca tctgcagtac
     1861 atgtcagatg tacaatataa ataccactgg cctgattttg ccgattttat cataactgat
     1921 ccaatcctga aaaaggatct ttcctatgcc cggttcatga tgtttatcgt caccagtatg
     1981 attggtatcg aagtggatct gcaggaggga cagatagaga atgcaagaaa tcatctatcc
     2041 cggttcgcct caagatttga gacgctgact gagagctgcg agttgtgcca tgcagaccag
     2101 agacaccact ttgtcgataa ctccatgcag gaagtcctgt tgagtttgca ggaggcattt
     2161 gaagcggagc cgatagacat ccagcttttg agaacatcac tgcagactct cggcacggaa
     2221 agctgctata aatgtcatct cgtacacatc cctccgacct atgccaaggc gcgctggaga
     2281 cccgcaggac ctcattaata gaccctggtc agccctcccg ggttgactca cagctgtaac
     2341 cagccacaac ctggattagt taaatttgct tgactaatcc aatttcgatg gttttcttgt
     2401 agatacacac ccggggcaga agcacctgcg acaagcatta cttaaacggt ggatctataa
     2461 ctgctattta tactttggag gtatatcatg caaaccacaa ggcttgtatt attgattgca
     2521 gtcttgatgc tttttattca cgtgattgcg ttatccgaag tcccgaatat gttcaactac
     2581 cagggacgcc tcctggatga tgcgggaaac ccgcttaacg gaacggtgcg tctagacttt
     2641 ggtatatatg ccgacacggg cggcacgacc cagttatggg tagagacgca tacgtcggtc
     2701 ccggttacag acggcctgtt caaggttcag ttgggatcgg ccacaacttt gccgaattca
     2761 gtgtttgacg gttcgcttcg atatttggga ataagagtga attcaggtac gatgctgcgt
     2821 ccattaacac ccatagtcgc ttcaccctat tcatttcgag cgctgcatgc tgatacagca
     2881 gcctatgcca tgagtgcgcc gggcggtgtt gacggctggt ctcagtcggg caactatgtc
     2941 tatctggctg atattggcga ctcggttggt atcggcacga tcagtcccgg ttcgcctctg
     3001 gaggtacagg gtgccaggac tgcgatagtc gggcaatcga cgggcagcgg tattatggcg
     3061 ggtgcgatta cgggattgaa tactggtagt ggtcatggag taatgggaat aagcagtgac
     3121 gggccgggtg tttatggatt gagcacgaac gggccgggtg ttcatggttt cagttcgagc
     3181 ggttttggtg gatactttga tgctcccaag cattatttta cagctaatgt cggaatcggc
     3241 actaccgagc cggagtatcc ccttgatgtg cattccagtg cggtttcagt gagaggctat
     3301 actaccggag ggccggggac atcaggcatc tatggtcgag ctgaaaataa tgcctatggt
     3361 gtgatgggca ccagcagcag cggccgcggc gtatacggcg ccagcacgac cggattcggc
     3421 ggttatttta tggggcccaa acattattta aatggtctga ccggattcgc gactgaggag
     3481 ccgacccatc gggttactgt caatggggct ataggaatac agcagagcgg gacaaccaaa
     3541 taccatctca attactacaa caatggtttt aatatttccg agacaaatgt tgccgactat
     3601 cgtttgtaca taaaagaagg cggtaatgtc ggtatcggta ccagcaaccc gactcagaag
     3661 ctatatgtca acggcaatgc ccatgttgcg gggaccttcc atgcagatgc atttgaagcc
     3721 aacgccatcg gcaaggagaa tattatcgat gaagttggac ttgcccatgc ctccaattca
     3781 ggctcttctt ctttgacaac gagttatgca gcttatctga ccaagcaaat aaccgttccc
     3841 actgcaggat atattctggc aatcgggtgc gcacatttca tgtttgatca cggggtcagt
     3901 ggaagcagcg gcggctatct tgcgttctcg gatcttcccg ataatattaa cggaggcatc
     3961 aatcagctgt ttatgcttgg gcacagtgtc tcggccgggt attaccgtgt ttcaattccc
     4021 tgccaacgcc ttttctatgt ttcaggtgca ggaacataca gcttctatgt aatcgcaaag
     4081 agagaggcgg acaattcggc aagtattgca gctccacaga tggccctgtt gtttatcccg
     4141 accgcttacg gcagcaagga tggagtgctt tctatcgacc cggaagacga catctacacg
     4201 ttctcgggga gacaggagct ggaggagaaa ctcactggtg ccagccaatt ggaggaacct
     4261 gttgccggat cgtcggccga aggtgacaat ggtgctctga ttaaacaggt caaatcgctg
     4321 acagcagaaa tcgaagtatt aaaaagccgg atggaggctt acgaaggcca ttagaataat
     4381 tcttatgggt tacgcctctt ccagcgcaag ctactcggca ctttggggga tttcaaggat
     4441 tacactgcgc attggtcctt atgcgcacgc ctgggtgcct ttgtatatcc attaatctat
     4501 ttttgcttga ccaatccagt ttttattgtt ttttacagat gcacaccggg tcataaacac
     4561 ctgcgacaag cgtaaatgag atggtagata cataacagct atttataatc aggaggttta
     4621 gcatgaaagc cgcaaaagtc tttctaacca ttgctgtttt tgtttttgta gtctttgtaa
     4681 gtctgattgc cgacgtccca cattttttta actatcaggg gcgtcttatg gatgatgccg
     4741 gcaatcccct gaacgctact gttgagctag atttcggcat atataccgga gaatccggaa
     4801 ccggtctgct ctggcaagag gtacatccat cagtttctgt agttgatggt ctctttaatg
     4861 tctatctcgg gacgggcaat atgcttcctg attccttatt cgatgggtcg gttctatggc
     4921 tgggaataaa ggtcaatgat ggtccgatca tgcagcccct cacgcctttt gtcgcatcac
     4981 cctattcgtt ccgcgctatt catgctgaca cggctgcata tgccttggat gcgccggccg
     5041 gatcgagcgt atggtcgcag tccgggaatt atgtcagact tccggatgtc ggcgattcag
     5101 tgggtatagg cgtgctcaat ccggtcgaga aattgcatgt aggaggcaac attctattag
     5161 gctcttatgg cgatatcagg ttcagcccgg ataacacgag aataaactgt gagtcaaacg
     5221 atctgcagat aaaagcgcag accgatcttt atcttgagcc ttacgatgat ctgtacatca
     5281 gaaggcggga tggcggttca tcctgggctc attttgacaa cagtactcag aggcttggaa
     5341 tcggtgtgct tgatc
//
LOCUS       DSEB01000328            5378 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001109, whole genome shotgun sequence.
ACCESSION   DSEB01000328 DSEB01000000
VERSION     DSEB01000328.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5378)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001109"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..253)
                     /locus_tag="ENO07_05965"
     CDS             complement(<1..253)
                     /locus_tag="ENO07_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01548.1"
                     /translation="MKKILNAVCITLALSIFACPGANADITVEMKSSVSGIPFLRVFE
                     INQITGIRGNDVITATEGQMDVGDTAISFRSSTYMNTVNE"
     gene            complement(278..1207)
                     /locus_tag="ENO07_05970"
     CDS             complement(278..1207)
                     /locus_tag="ENO07_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01549.1"
                     /translation="MKITVWLLVVGVMLCQTARADLTVDKQITITDPVSGKTVSHETV
                     LVREDDYAVNDMQFQGHEVRTVVKGHGEELLLINYEDLTYSLFDDYLIDSLISGRKGL
                     DASESDSIRKHIKLNRLELDFTGRTRDFYGIEARELQISVDLTAKAPLLDESIMPLRI
                     VIRGNEWISTDFPVADTYSHSRRFLSGMPSSMTRNLFGDYSEILRSMDASDSVIDHVF
                     DIFTYLVLEGSLYLTIEMGASGQSDLKGEPVPAMSWTMKFETSCLNISFDKVLDEEFE
                     IPRGFRKVHNAFSKIGTFVIPGLEKWFALDKDV"
     gene            complement(1271..2173)
                     /locus_tag="ENO07_05975"
     CDS             complement(1271..2173)
                     /locus_tag="ENO07_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01550.1"
                     /translation="MRFCKALVLAALISVFLCSAVSADFTAKTKTTTSGIMGMGGMTS
                     GTTYLIKGDEVAIASTMEMPSMGQGQKGGNTKMILRKNGKELVMINYNQGTYSILDEK
                     LIDSMAAVIGNLGGLADSLKEQMTVQTMEAKITDSTRKIQDLKAEEMSLNMHVVMHVQ
                     MMGPEPTQIDVKMTGSMWGTKDFDEHGTYAKMIEDISALMMSGSGGGFSGLMPLMETF
                     GIDQATMDEVMKFALYIPLEGAFNVKMEVPNMPMNVTMNTELIETSKEAIPDSEFEIP
                     EDMEQVEPDLDFSSGGMGFPGMGQ"
     gene            complement(2249..2683)
                     /locus_tag="ENO07_05980"
     CDS             complement(2249..2683)
                     /locus_tag="ENO07_05980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDS01551.1"
                     /translation="MPNRRKHDHIGAYNFKIEIDGVTQAAFKAVDGLDAETEVIEYQD
                     GDDLTLRKRPGQTRYSNIILKRGYVNTQELWLWRKAVMEGRVERKSGSVIICDDSGNE
                     IMRYNFFEAWPCKWRGFDLDGRNSATAVEEIEIAVERLERAV"
     gene            complement(2733..3950)
                     /locus_tag="ENO07_05985"
     CDS             complement(2733..3950)
                     /locus_tag="ENO07_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HDS01552.1"
                     /translation="MPEYLSPGVYVEEVDKGTKPLEAVGTSTAGFIGQSKQGPVNEAT
                     LITNWGQFTRTFGEFADSPYLAHAVYGFFNNGGARCFVTNVGMEGAPPPPPPPKKEDE
                     DKKSPPPPSPGSKGGPSPALFIGKDGGPGNKTGLVTFEDIDEIAIVAAPGMTEPAIQD
                     AVISHCEKMNRFAILDSEETIEKGGVDKIKKPRDSQYAGFYFPWIEVYDPAKDKNVYV
                     PPSGHMAGIYARSDNTRGVHKAPANEVVRGALGLRYTISKGEQDILNPRGINCIRAFR
                     DRGIRVWGARTVSSDPSWRYINVRRLFIMVEESIKNGTQWVVFEPNDHTLWKRIKRNI
                     TAFLLRVWNDGALFGQTPEQAFYVKCDEETNPPEVVDAGMVVIEIGMAPVKPAEFVIF
                     RISQWQSGSSVNE"
     gene            complement(3995..4585)
                     /locus_tag="ENO07_05990"
     CDS             complement(3995..4585)
                     /locus_tag="ENO07_05990"
                     /inference="COORDINATES: protein motif:HMM:PF14065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HDS01553.1"
                     /translation="MNYNVIQLVTTSVLSLLKEDLKRNDIKGVNCLNRQPNAQNLAGE
                     MPCCLFYLYGISENQELKERDPYQVRDKDADGNTYEFLREAPIKLDLNYLLCTYAEDI
                     DMEQKILGRCILTLYDNSELSRLDDLKKLAKHPSDRIGMRMLPISREEQVRFWSSLRE
                     PMRPAAFYEVTVRMESERQRKFRRVEERVLDIHQKE"
     gene            complement(4883..5362)
                     /locus_tag="ENO07_05995"
     CDS             complement(4883..5362)
                     /locus_tag="ENO07_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01554.1"
                     /translation="MEIKRTGKTKEIDGFKCEEIYFKLDKSSSTGVGEAKVKHYFEGT
                     MWVTNDIPNGELYYNYNAFAQNYFRGSNYNSGGFFDILARLDVDSYNLIRLVEALNGV
                     PVQASFVAQLPSATGGNVFETKIKLIEHSTEPFEKGHFTAPEDEKYNQVPVGEFRAF"
BASE COUNT         1316 a         1456 c         1165 g         1441 t
ORIGIN      
        1 gctcgttcac agtattcata taggtgcttg acctgaacga gatggcagta tccccaacat
       61 ccatctgccc ttcggtcgca gtgataacat catttccgcg aatgcctgtt atctggttaa
      121 tctcgaatac tctcagaaac gggattccgg acacagagga tttcatctct accgtgatgt
      181 cggcgttcgc gccggggcat gcaaaaatac tgagcgccag agtaatacat accgcattta
      241 atatcttttt catctctcgt cctgttgttt atttttatta tacatcttta tccagggcaa
      301 accatttctc aagtccgggt ataacaaaag tcccgatttt gctaaaggca ttgtggacct
      361 ttcgaaatcc tctggggatc tcgaattcct catccagtac tttatcaaac gagatattga
      421 ggcagcttgt ttcaaacttc atggtccagg acatcgcggg cacaggctcg cccttgaggt
      481 cactctgacc ggacgctccc atctctattg tcagatacaa gctgccttcc agcacaagat
      541 aagtgaaaat atcaaacaca tggtcaatta cgctgtccga agcatccata ctgcgaagaa
      601 tttcagaata atcaccgaag agatttcttg tcatagagga gggcatcccg ctgagaaatc
      661 gccgggagtg agaatatgta tcggccacag gaaaatcggt cgaaatccat tcattccccc
      721 tgatgacaat tcgaagcggc atgatgctct catcaagaag cggggctttg gccgtcaggt
      781 ctaccgagat ctgaagttcc ctggcctcta tgccataaaa atctcttgtt cttcctgtga
      841 aatcgagctc aagccgattg agctttatat gttttctgat actgtcgctt tctgaagcat
      901 ccagtccctt tcttcccgag atcagggaat cgatcagata gtcatcgaaa agcgaatatg
      961 tcaaatcctc gtaatttatc agcagcaatt cctctccatg acctttaaca acggttcgta
     1021 cctcatgacc ctggaattgc atatcgttga cagcatagtc atcctctctt accagcacag
     1081 tctcatggct gacagtcttc ccgctgaccg gatcagtgat ggtaatctgc ttatccaccg
     1141 tcaagtcggc ccgagctgtc tgacagagca tgactcctac cacaaggagc caaactgtaa
     1201 tcttcattct cgccttcaca aatatcaata ttccccgctt actcaagcag tataatatct
     1261 gatctgcacg ttactgtccc ataccgggga agcccattcc tccactggag aagtccaaat
     1321 cgggctcaac ctgctccatg tcttccggaa tctcaaattc tgagtcgggg atagcctctt
     1381 ttgaagtttc aattaattcc gtgttcatgg tcacattcat gggcatattc ggcacctcca
     1441 tcttcacgtt gaacgcgcct tcaagcggaa tgtataaggc aaacttcata acctcatcca
     1501 tggtcgcctg gtcgatccca aaagtttcca tcaagggcat caggcccgag aagcccccac
     1561 cggaaccgct catcatcaaa gccgagatat cctcgatcat tttggcataa gtaccgtgtt
     1621 catcaaaatc tttggtcccc cacatgcttc cggtcatttt cacatcaatc tgggtcggtt
     1681 caggtcccat catctgaacg tgcatgacca catgcatatt cagcgacatc tcctcagctt
     1741 ttagatcctg gattttcctg gtgctgtcgg ttattttagc ttccatggtc tggacagtca
     1801 tttgctcctt taaactgtcg gccaaaccgc cgagatttcc gatcaccgca gccatggaat
     1861 caatcaactt ctcatccaga atggaatatg tcccctgatt gtagttgatc ataaccagtt
     1921 ccttgccatt tttgcgaagg atcatttttg tattgccgcc tttttggccc tgtcccatcg
     1981 aaggcatttc catcgtagaa gcaatcgcaa cctcatcacc cttgataaga tatgtagtcc
     2041 ctgaagtcat gcctcccata cccataattc ccgatgttgt ggtcttggtc tttgcagtga
     2101 aatcggctga gacagcactg caaagaaaaa cgctaatcaa tgcggccaga acgagggcct
     2161 tgcagaatct cacatcaacc tcctcctgaa gggttaaatt agcctggatt tctccagctc
     2221 ctttgcgaag ctgtagaatc atgatctttt atacagctct ttcgagcctt tcaaccgcta
     2281 tttcaatctc ttctacagcc gttgcggaat tgcggccatc caggtcgaaa ccgcgccatt
     2341 tgcatggcca ggcctcgaag aaattatagc gcatgatctc gttccccgag tcatcgcaaa
     2401 ttatgacgct tccgcttttc ctttcgaccc gtccttccat cacggccttg cgccacagcc
     2461 acagttcctg agtgttaacg taaccgcgct tcaaaatgat attggagtag cgcgtttgcc
     2521 cgggacgctt gcgaagggta agatcgtcgc cgtcctggta ctctataact tcagtttcgg
     2581 catccaggcc atccacggcc ttgaaggctg cctgggtaac gccatcaatc tctatcttga
     2641 aattatatgc gccgatatga tcatgtttcc ttctattcgg cataatttcc tcctgtatta
     2701 attaccgccg gcattacctg ccggattata tattattcat taacagacga cccggactgc
     2761 cactgggaaa tccggaagat tacaaattcg gccggcttca ccggagccat gccgatctca
     2821 atcaccacca taccggcatc cacaacctcc ggcggattgg tctcctcatc gcacttgaca
     2881 tagaaggcct gttccggagt ctggccgaaa agagccccat cattccagac acgcagcagg
     2941 aaggcggtga tattgcgctt gatgcgtttc cagagagtat gatcgttggg ctcgaacact
     3001 acccactggg tgccgttctt gatcgactct tccaccataa taaaaagacg gcgcacattg
     3061 atgtatctcc aggagggatc ggatgatacg gtgcgcgctc cccatacacg gatgccgcgg
     3121 tcacggaagg cccggataca gttgatgccg cgtggattca gtatgtcctg ctcgcccttg
     3181 gaaatagtat acctcagacc caatgctccc ctgacaacct cgttggcggg agccttgtga
     3241 acaccgcggg tattgtcgct gcgggcataa attccggcca tatgacctga cggaggaaca
     3301 tacacatttt tatccttggc cgggtcataa acctcgatcc aggggaagta gaaacctgcg
     3361 tactgggaat cacgcggctt tttgatttta tccacgccgc ctttctcgat ggtctcctcc
     3421 gaatcgagaa tggcgaaacg attcattttt tcacaatgag atatgaccgc atcctggatt
     3481 gcaggctcgg tcatacccgg cgccgccact atcgctattt cgtcgatatc ctcgaatgta
     3541 accagcccgg tcttgtttcc gggcccgcca tctttgccga taaatagcgc tggcgagggc
     3601 cctcccttgg agccgggaga aggcggcgga ggcgactttt tatcctcgtc ttccttcttc
     3661 ggcggaggag gcgggggagg agcgccctcc ataccgacat ttgtcacaaa acagcgggct
     3721 ccgccattat tgaagaaacc gtagaccgca tgtgccagat aggggctgtc cgcgaattcg
     3781 ccaaacgtcc gtgtaaactg gccccaattc gttataagag tggcctcgtt aacaggcccc
     3841 tgcttggact gcccgataaa tcccgctgtc gaggtcccca ccgcctcgag cggcttggtt
     3901 cctttgtcaa cctcttcaac gtatacaccc ggagacaaat attccggcat aaaaacctcc
     3961 atgttttaga aaaggtattc agtttatatt aatttcattc cttctgatgt atatccagaa
     4021 ctctttcctc aactctgcgg aacttcctct gcctctcgga ttccatccgc accgtgactt
     4081 cgtaaaaagc ggccgggcgc atcggctccc ggagagagga ccagaacctg acttgctcct
     4141 cccggctgat cggcaacatc ctcatgccga tcctgtcgga gggatgtttg gccagcttct
     4201 tcagatcgtc gagcctggaa agttcggaat tatcataaag tgtaagaata cagcgtccga
     4261 gaatcttctg ctccatatca atatcttccg catatgtgca gagcaggtaa ttgagatcca
     4321 gtttgatagg ggcctcccgc aggaattcgt aggtgttacc gtcagcatcc ttatccctga
     4381 cctgatatgg atcacgctcc ttgagttcct ggttctcgct gataccgtac agataaaaga
     4441 ggcagcaggg catttcaccg gcaagattct gagcattcgg ctgccgattc agacagttca
     4501 cccctttgat atcgttccgc tttaaatcct ccttgagcag gcttaatacg gaagtcgtta
     4561 ctaactgaat gacgttataa ttcatcctcg agtatggagt ggtctcgctt caatgactga
     4621 tatattatct cgattattcg gatcgcaatt gacgcctaat aataataaag gacccgtttt
     4681 ccatcaataa aaaacattat aaaagagcca taaaatccga tttaaatgca cacaaataga
     4741 aggtatttca tgagctgctc acggccagca tccttgatga tgacagcctt aataaaaaag
     4801 cccgcccaaa aactcaattt gggcgggaaa tctatccaat ttttcttaag atcccgtctt
     4861 taatgagata attttatcgg atttagaaag ctcgaaattc tccaaccgga acctgattat
     4921 atttctcatc ctcaggggca gtgaaatgcc ctttctcaaa cggctcggtg gaatgttcga
     4981 tcagcttaat ctttgtctcg aatacatttc cacctgtggc cgagggcagc tgcgccacaa
     5041 atgacgcctg caccggaacg ccattgagtg cttctaccag tcgaatcaaa ttatacgagt
     5101 caacatccag gcgggcgaga atatcgaaaa atccgccgga gttgtaattg gagcccctga
     5161 aataattctg tgcaaaggca ttgtagttgt agtaaagctc accattggga atgtcgttgg
     5221 tgacccacat tgtgccctcg aaataatgct tcaccttggc ctcgccgact cccgtgctcg
     5281 aagatttatc cagtttaaag tatatctctt cgcatttgaa gccgtcgatt tccttggttt
     5341 tacccgtgcg cttgatttcc atctgcggac ccttggca
//
LOCUS       DSEB01000329            2000 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1010099, whole genome shotgun sequence.
ACCESSION   DSEB01000329 DSEB01000000
VERSION     DSEB01000329.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1010099"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
BASE COUNT          476 a          564 c          471 g          489 t
ORIGIN      
        1 cgctgtaaac caatacctgt ggtcagttcg atattgctcc tgggtcgcgt attgacatca
       61 aaacctatac tgcgatgatt tccgccgcga tcatcgccgc cgcccacccg caggtggaag
      121 tagacattct tgcgctgatc ggtattgata tttaaccatg acgctgcggt gggatagacc
      181 ggccactgcc agattccgtt gccgcgagtc tccagatcgc tgaacttttc ggtctgaacc
      241 gccaggctcc attccatcga ccagtagttg gtgaattcaa tatatgtatt gaaattcccg
      301 cccagctcat ggttgatgcc ctcataattc caggatgcat ggaagttcag attattccag
      361 ctattacgga ttatccacca gtcatcacgg gtccggtact ggaaccagga ccagactgcc
      421 cgctggttat tgcgacccag atatcccagg cgattgagat ttagattggg atccataatc
      481 tccactccaa cagctccgcg gaaatgatcg cccgcagtct tctctatcgc ggcatccaga
      541 ccaaagccgg ttttctgatc atcaagccgg ctgaaaactg tctgaccgct caagacccac
      601 ctgctgttat tggtggccag ccgccagtcg acgccgccgg ttacggccgg atggaaactc
      661 tcctgggacg ccatggtgaa catgccgccc acatgcgaat ttccaaagat atcctgcttt
      721 acacgcagca cagaataatt cgctgacttt tccaccactc cgcttcgaaa ggcggtatcc
      781 agcggaacat tttcaacaac cgcttcacca ttgacccagg ttgtatcctc actccagttg
      841 gaaaagacag catactctgc ctcttcctcg gctgttacag cattaaggaa tgctaccgac
      901 gtgccggacg acaatttgcc ggtcagtttg gctgcgccca aaatggtggt ttcattggga
      961 tagtctttat aaaatccaaa atcttcatca tcgacgtgct gcctgggcgg ccgtccgatt
     1021 cttctcgaat aaaacagcga aaactccgta cgaaataaat cggagccctc cagaaagaag
     1081 ggcctttttt cggggaagta cgtttcatag gcagagaggt tcagcaccgg ctgatcgagt
     1141 tcaacctgtc cgaaatcagg attgatggtc gcatcaagaa tcaggctgga tgataatgca
     1201 tatttcatat cgaagcccat gtcgcccata aattcccggc cgtcaggatt accggcggtt
     1261 tttggctctg tctccgtact cgagacagcg tagggcaaaa gctctattcg cccgtggaaa
     1321 tgaatccccg tcaaacccgt aagatgtccg aaattggata caaaaccacc cttggagggc
     1381 ggtgtgaagg cccagatgtc ggtctcgctc ttatgactga tatagcgctg gaaatctata
     1441 ccccacgtat gttcttgctt ctccgtgaac ctgaggcagt gatatggaat tttaaattct
     1501 gccgcccagc cccagggatg tcttttgact tccgcctccc agaccgcatc ccaggacatg
     1561 tctgagccga catcattata atagcggcag tcgcgcagga ctcccgccga actgacctcg
     1621 aaagcatgac cgttctgatg gtcatgaaaa ggatccaggc ggactgtcac acggtccgat
     1681 tcggaccagc ggtcacgcct cacaagctga ttgattatct tatccggctc atcgtcgtag
     1741 caccagaatg ccacatagac tgcattatcg tcataggcaa ccgctaccat agtcgactcg
     1801 gacgcttctt ttccttcatc cggctcgcgc tgagtgaatt ttttcgccag cacaagctct
     1861 tcatgctgcc agacagaatc gtcaagattg ccgtcgatta cgggaggatg cgggtttatg
     1921 cgaaatgctt ctatttccgg gactaatctg tccccttctg atgtcccctg tgcagataaa
     1981 gcgagcgccc acgccataac
//
LOCUS       DSEB01000330            3604 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002752, whole genome shotgun sequence.
ACCESSION   DSEB01000330 DSEB01000000
VERSION     DSEB01000330.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002752"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
BASE COUNT          899 a          868 c          923 g          914 t
ORIGIN      
        1 gctctgggac gagacgatcg aggatttcaa agtcaaaact ctaatactcg agggaacagg
       61 ccagacctgg gaccgggact tccagaatgc gtacggggaa tatatgaatc tggatccggg
      121 tccgccgttt attattcagt gcaatgtgct tattgaaaac atccacgatg aggacggtaa
      181 tgtcacggac tggggatccc tgaacatcga gagcggagga gaaatccttg atcctttcga
      241 gattatcttt gctcccgatg aagaccagac gtactgtgag ggtgaaatcc gaagctacgg
      301 aaaccttaat tttgtgggga ctcccaccga cccgatcata ttcaagggaa agtacaaaat
      361 tcgtacacac cgcccgggta atcccgcccc tgatagctta gtaaatgaat ctcagataca
      421 tttcgaatac tgtgtgttcg actactggga accatatatc gccggtccac ctgttaattt
      481 cgacggcggc catgtttccg tagaaaattg catttttaga aatttccatg attgttccgg
      541 ggttctgagc ttttacgatc ctctctacgc cagcactcag ggcccggtct ggaactgcat
      601 tgtccgcaac tgcttctttg ataataattc tgttgtgggt cagcatctcg tcagtatgca
      661 gcacggcagg tttgtaatag acaacaacag gttcgaatcg aacataaccg aggctacgga
      721 taattcatcg atgatttata ttcttggtca catcgacact ctaaaaaata acgcggcttt
      781 gaacaacact gtcaactccc tcatcttcta ccatgtagtt accggcgcgc attcttacat
      841 taaatccagc gatgatctgc cgattgtagt tgacgacatg tttattcacg aggattctac
      901 ccttgtaatc ggccccggtt cagttattaa accgtatcat tacggcgact ggccaagaaa
      961 cgtatgggcc catgtcaacg gacatatgaa aatcgagaat gcaataatca cttcctggca
     1021 gaatgacgag gtgggaggtg atgttgatct gcagccacaa cctgattata tcccgagatg
     1081 caactacata tatgtagata caggcggttc ggttgagatc accaataccg atatcagtta
     1141 tgccgaaaaa ggtgtcgaag cgatcggcga tgtcgtcatg gatagctgcc ggttgcagat
     1201 tatcaaggat gaagggatag aatttcggcc gataggcaac aacattctgc aggtctcgaa
     1261 tactacaatt cgggcagtcg ggaattccgg catcgtggtg gacgcccgga acagccaggg
     1321 gctggaggtc agcctcgaca gcgtcagcgt aatccacacc gtcggggatg ggctggaact
     1381 gtatctttca gaatgctcac atgtgatgag tgtcgatatt aacggatgta tcatagctaa
     1441 caacaacggg tctggtattt attgccccgc ggactttggc ttgaactgct tcaatgtctc
     1501 caattccatc attgccggca actacaattc gggcattttg ctctacaaat ccggtgctga
     1561 gacggtcaac gtgaacctcc attccaatgc tgttataggg caccgcctgg acggcatttc
     1621 cgtgcaggcc ggcactccgt ccatcacaaa caatacagtc gggcaaaatg gccgcaaagg
     1681 catagcgatt agttccttta tatatcaacc tggatctatg ctggttgcga ataatatcct
     1741 tataaaaaac cagcgaggct ttgaaaaggg ccccgaggag ctttgccctt tccatcacaa
     1801 tgtcttctgg gacaatgaaa ttaccttcta tttcaatacc cccgatggag gtttgtggac
     1861 gatcgaagag ctccaggcat tgggaggaga ttatgtcacc aacatgcaaa ctgatccaga
     1921 gtttctttgt gaagtgaatg gtacagtaga tactgtcatc tgggatgaaa agagaaatat
     1981 ttcgatcgtg gtggatggct ccgccgattt cggagaatta gatcatcaat acgcaatcta
     2041 ccaggtggat accagttgga atatctggtt tatggtttcc gaggtgaagg atgattcaat
     2101 cttaattgac gctgatatta ccgagtcagt caataccggt gactcctata ttattttcga
     2161 ttaccatctg gcgccgtctt cacccctgat cgaagccggc aacaacccgg tggtcgcggt
     2221 tgcgacggat atcgatggtg aagagcgcat aatggacgga gatgaaagcg gtacagcagt
     2281 ggtggatatc ggagccgatg aattcaatcc ttatccggac gccccggagc caatcgacat
     2341 catctgcccg gtcgccggcc aggtctgtct tgcaggggat acgattgtca tcgaatggac
     2401 cgctgaagat gctaattcgg ttgatatctt ttatacgctt gattatcaac agggagagat
     2461 agtctggctt cccatcgcct ggagtcatcc ggctgactcc aacagcgttc catggcgaac
     2521 ccctcctgac gcgttggcgg cgaaatgttg tatcaggatt cagaattcag acgatgagaa
     2581 ccaccgggcc aaaagcgacc tgttccgcat caaaccctac ctgctcacgc gttttgacgc
     2641 cgacagtacc tatgatgcct atgcatccga tttggacgga tggtcaattc ccaatgattc
     2701 ggcttccatg tggccggttg aatggtacag tcagtttgat tatgcagagg gcatagaccc
     2761 cttcacggag gacgaatatc cgcttttctt cgagtggttg ggcgccgaat ccaagtcttt
     2821 tccagactgg cctttgtggg tgcaggcatt tggtatagat gcttgctttt acagcattcc
     2881 cggcaggctc ctccataaac ccgcaacagt gctttcatgg gcggagataa ttgatgagtg
     2941 gggaggttca tgcgcgggac tcacggcttc gagtctggct gcttttgatg attcagcact
     3001 gttcaagctg actttcccgg aagccggtaa cttttctgat ctctatacgc tcgcggtcaa
     3061 tgacgaccgg cgcctggtga taaaccttgt atggacacgc ctttttgcca aatccgcctg
     3121 ggatcatctt gtagctaact cgacgaagag accggcacag acgctgaagg aaatacgaga
     3181 tatgcttctg gcagatgaac gtctcgatgc cgacctgtac attgagcacg attctttgct
     3241 ggaggcccac gccgtcatgc cgtatcgtgt tgaccaggac agtgcaaatc ctaatatctg
     3301 gcgactgcgt gtgtacgaca gcaatgctcc gggggatacg aaccgtgtga tcgaaataga
     3361 taccgcggca gaacactggg attatttgcc catgccgggt tatcagggct ctgcgggatt
     3421 cttcctgcgc aatccggccc tcgatttcct tgatatcatg gagctgggtg ccggcgcgcg
     3481 aagcgctgtt ccggttcctc gtttggcgga caggtcagat tggatgacag tcggcctgcg
     3541 taacacggat aatgttacaa tcagggcaca ttcaggagat tcggcgggat tctcactcga
     3601 cgca
//
LOCUS       DSEB01000331            3601 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002756, whole genome shotgun sequence.
ACCESSION   DSEB01000331 DSEB01000000
VERSION     DSEB01000331.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002756"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            183..1760
                     /locus_tag="ENO07_06000"
     CDS             183..1760
                     /locus_tag="ENO07_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01555.1"
                     /translation="MKRMYLFILSPFLIIVMPFLDLSASAHRAPDPLFESYVEAVEAG
                     DWEAAEKCWLPEEVEKSKRLEITYQGVKAKYDCSSPLISLQAEISNGRVEVEIGKLEI
                     SDTLAVFDILLLAGGDTITVKYYALEKDAEWFLVYPMTALASGWDRLQTEYADIFYSD
                     KSMINDYAVDGLDDYIEFLIEFFELNPDKIDRLRTEKIDYYLCTKEDFKNITGYDAHG
                     LAYLSADAIITRHLPHPHELTHLIINYTLDSVPLYTLPFLQEGLACMCGGRWGKSPEV
                     INQLGSAILKNDLCNLDDILTLQGFNVTVGMPDISYPVSSLFSGYLVEEMGIQDFKRL
                     YLELSGSSEEVDDYSKEYVISKIWALSGLPFDSIRVGFLEYSAMHEGGGIFVYEDNPD
                     AVSIQELTSDSLEIEISESLDCYNFIIGGVKDKLDGTILLFDDSIRLPDQYQSWMFAE
                     QLPEHEYDGAVCGIKFDINEAGFYDYRTNILEAKFVSSFFPEAPYYDPVNKTVKFSLR
                     KPLIPKSLESYKIILSD"
     gene            1978..2490
                     /locus_tag="ENO07_06005"
                     /pseudo
     CDS             1978..2490
                     /locus_tag="ENO07_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012410977.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
     gene            2940..>3601
                     /locus_tag="ENO07_06010"
     CDS             2940..>3601
                     /locus_tag="ENO07_06010"
                     /inference="COORDINATES: protein motif:HMM:PF07719.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDS01556.1"
                     /translation="MKKATSLFVQTLIIILLSINLAAAQDQPETDFDAPYLIIAADSL
                     FNADQYEKAREWYMQAAEKAETDSLNSELTEANAMIARTYLVQGYVDEGMEYLRKTLQ
                     TATPDDPMGWSRYLSVRGRFEWNNGTLDKATETFKEMFYYCQEHELWDRAVDAAHMVA
                     ITSDLPGQVEWAHKGIEVAEKHEVTSWLGPLWNNLGATYEDLEQYPEALQAYKKAREY
                     HY"
BASE COUNT         1032 a          747 c          896 g          926 t
ORIGIN      
        1 gcgacaggat aagaaagagg cccgtcgcca gcagcgcgag tgggataaga aaatccgtca
       61 atatcatcag cagatgaagg agctgcggaa gcgcagaggc tattagaaac actgtttttt
      121 tgaagaaatg ccgtgtatcg gatgtataat cagtatcaga tcaacgtccg aggttttgcc
      181 gaatgaaaag aatgtacctg tttattctat cacctttttt gattattgtt atgccctttc
      241 tcgatttgag tgcctctgca catcgggccc ctgatcccct gtttgagtca tacgttgagg
      301 cagtagaagc gggggattgg gaggctgccg aaaaatgctg gcttcctgaa gaggttgaaa
      361 aatcaaaacg tctggaaatt acctatcaag gagttaaagc gaaatacgac tgctcctcac
      421 cgctgattag cttacaagcg gaaatttcaa atggccgggt tgaagtcgaa atcggcaagc
      481 ttgaaataag tgacacgctt gcagtatttg atattctcct tcttgcggga ggagacacca
      541 tcacggtaaa atattatgcc ttggaaaaag atgctgaatg gtttctggtt tatcccatga
      601 ccgctctggc atccgggtgg gacaggcttc aaactgaata tgcggacatc ttctacagtg
      661 ataagagcat gataaatgat tatgctgttg atgggctcga tgattacata gaatttctga
      721 tagagttttt cgagcttaat ccagacaaaa tcgaccggtt gcgaacagag aagatcgatt
      781 attatctctg cacaaaggag gatttcaaga atattaccgg ttatgatgct cacggcctgg
      841 cgtatctgtc agcggatgca ataattacaa gacatcttcc gcacccccat gaactgacac
      901 atctcatcat aaactatacc ctcgattcgg taccgctcta caccttgcct tttttgcagg
      961 aggggctggc atgtatgtgc ggcggcagat ggggcaagtc tcctgaagtc ataaatcagc
     1021 ttggaagcgc gatcctcaag aacgaccttt gcaacctgga tgatatcctc actttgcagg
     1081 gatttaatgt aactgtcggt atgccggata taagctatcc cgtgtcatca ttattctcag
     1141 gatacctggt cgaagagatg ggcatacagg atttcaagcg cctttatctt gagctatcgg
     1201 gaagttcaga ggaggtcgat gattactcta aggaatatgt tatctcgaaa atatgggccc
     1261 tttcgggatt gccctttgat tccataaggg taggatttct ggaatattct gcgatgcatg
     1321 agggtggggg catattcgta tatgaggata atcctgatgc ggtgagcatt caagaattaa
     1381 cgtcggacag cctcgaaata gagatttcag agtccctaga ctgctataat ttcataattg
     1441 gcggtgtaaa ggacaagctc gatggtacta ttcttctttt tgatgattct atcagactcc
     1501 ccgatcagta tcaaagctgg atgtttgcgg agcagctgcc ggagcacgaa tatgacggcg
     1561 ctgtctgtgg aattaaattt gatattaacg aggccggttt ctatgattat aggacaaata
     1621 ttctggaagc aaaatttgtt tcgagcttct tcccggaagc cccttattat gaccccgtta
     1681 acaaaaccgt caaattcagt ttgcgaaaac cgttgattcc caaatccctg gagagttata
     1741 agattattct ctctgactga cccctgcaat aaattcattc agacataaat gcccggaacg
     1801 tctcagccat ttgtcttgtt tttcattccg taacatatga aaaaaatttg ggccgttgaa
     1861 ataatggagg aggatatgaa atttaaaatt atatcaacaa cgattttggg aatgatcttt
     1921 attctttccg cagtactggt tggtcaatca agtatgacaa aaattccggt gccgggaatg
     1981 gacgaaaaga aggcggaaaa gactgataaa ttgaaccata tgaagataca gttcaatacg
     2041 attcagggcg ataccgtcag ccttgaggat tataaaggga atgtcgtact gatcgtcaat
     2101 gtcgccagcg aatgcggtta tactccacag tatgccgacc tggaaaagct gtaccaaaaa
     2161 tatgaagata agggattggt ggtgataggc tttccctcca acagcttcaa ctaggagccg
     2221 ggcacagacg agcagattta taaattctgc accagcaagt ttgatgtcac ctttccgatg
     2281 atgtccaagg tcagcgtcaa gggcgaggac cagcatcccc tgtttgttta cctgacagag
     2341 ggcaccgata tgaaaggcga gatcaaatgg aatttcgaga aattcattct tgataagagt
     2401 ggtaatctgg tggcaagatt cgccaccaag gtcagcccgc ttgatgatga agtcgtggcg
     2461 gtgattgaaa aagagctgga agcgaagtag gaaagtatac aggtaaaata tataagccgg
     2521 aattgcatgt ttccggcttt tgttatagcg gccgaatgta gttccgggtg gcacgcccaa
     2581 atttatttgg gcgtgttttg aattggatct tacaagggtg cccgccacaa atttatttgt
     2641 ggcggatgca gcgccgacaa aacaagtttg tcggcggcac ccattatagg aaaagcaaca
     2701 cctggccaaa cagagtttga gcaggccacc cataaaggcg tcagttcacc gcctgcggct
     2761 aaggaactga cgcaacagct tatgagatag cccgaaatcg ttgcggcaga tcctcagatc
     2821 tgccgcttta tgtaaatgtg acagaccacc tgattcaacc ggctccccac ttctgtcttg
     2881 acaaaaactg tcgggtttgc gataatattg tattaacttc atccggagga gaaaatctca
     2941 tgaaaaaagc gacatcatta tttgtgcaaa cattgataat tatcctgctt tcgataaatc
     3001 ttgccgcagc tcaggatcaa cctgagacag attttgatgc tccatatctg attattgcag
     3061 ccgacagcct gttcaatgcg gatcaatacg agaaagcgcg tgaatggtat atgcaggcgg
     3121 ccgagaaagc cgaaacagac agcctgaata gtgagttgac cgaagccaat gccatgatag
     3181 caaggaccta tttggtgcag ggttatgtag atgagggaat ggagtatcta aggaagacac
     3241 ttcagacagc gactcccgat gatcccatgg gatggtcgcg gtatctcagt gtacggggcc
     3301 gttttgaatg gaacaatgga accctggata aggctacaga aacattcaag gagatgttct
     3361 attattgtca ggagcatgaa ctctgggatc gcgcagtcga tgccgctcat atggtggcga
     3421 taaccagcga tcttcccggc caggttgaat gggcgcacaa ggggatagag gttgccgaga
     3481 aacatgaggt gacctcatgg ctggggccgc tctggaataa tctgggcgca acatatgaag
     3541 acctcgagca ataccccgag gcactgcagg catacaaaaa agcccgtgaa taccactata
     3601 a
//
LOCUS       DSEB01000332            3318 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003293, whole genome shotgun sequence.
ACCESSION   DSEB01000332 DSEB01000000
VERSION     DSEB01000332.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003293"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..785)
                     /locus_tag="ENO07_06015"
     CDS             complement(<1..785)
                     /locus_tag="ENO07_06015"
                     /inference="COORDINATES: protein motif:HMM:PF05368.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDS01557.1"
                     /translation="MKESADSQTALVTGANGFIGSHLCRELVNHGFRVRALVRKTSNL
                     QFLENLDLELVFGEITQPLSLPEAVRGVDYVFHPAGLVKARNRETYFSINRDGTRNLV
                     EAVLATAPDIKRFVHVSSQAAAGPSRSMTAVLESDPPSPVSFYGESKLASEKEVLKRK
                     DKIPVTIIRPPAVYGPRDIDVYKFFRLAKAGINPSIGIDPTYVSIVHVHDLVRCIRMA
                     SLSDKAAGETFFACNREWYSMDTLMRVIGKAVNRQPIKIAIPIP"
     gene            855..2066
                     /locus_tag="ENO07_06020"
     CDS             855..2066
                     /locus_tag="ENO07_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HDS01558.1"
                     /translation="MQGSEQVKIRDIFDKCREFTRATEVKQAGYYPYFNPISSAAANT
                     VMIDGKELIMIGSNNYLGLTTHPKVIEASREAVKKYGVGCTGSRFLNGTLDIHLELEE
                     ELAKFLRKEAVLVFSTGFQTNLGTISCLVNKNDLILADRSDHASIVDGCRLAFGKTLK
                     YKHNDIEDLERILVSRNNGDQGILLVVDGVFSMEGDIVNLPQLVKLRERFGFRLMVDD
                     AHSLGVLGEDGSGTATHFGLNDQVDLIMGTFSKSFASIGGVIAADAYVINYIKHFSRP
                     LIFSAALPPSAVAAVLECLKIIKAEPERREKLLLNGKKMRDGLRAMGYSIGTTETPIV
                     PVYIGEDMACFKFWRTLYDAGIFANAIIAPAVNPGNALIRTSYTATHTDEQLDFVLET
                     FEKVGKKLGLI"
     gene            2075..3226
                     /locus_tag="ENO07_06025"
     CDS             2075..3226
                     /locus_tag="ENO07_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDS01559.1"
                     /translation="MSFENVSIIEVKSSADMDRFIKFPFKLYADDENWVPPLISERRE
                     FFDKSKNPFFRYARVKYFMAEKDGRIAGRIASIVNFNHNAFHEDTVGFFGFFECIDDY
                     QVAQVLLRVALIELKKEGMTVMRGPTNFSTNYELGFVIDDFDAPPVINMPYNPGYYIS
                     FMDRFGFQKAKDLYAFKITRENKPPERVEKIVERIRKKENVNVRNLDLKDFDNELKIV
                     NKIYNNAWSRNWGFIPLPDDEFLHIARDMRQIVDPDLVFIAEVDGNPIGFSMALPNIY
                     QVLPYAKGRLFPFGALKLLWHTKIKNKINSMRVITMGIEHEYQKRGIDNIFYLDTFKR
                     GYDKGYEWAEISWVLEDNQLMIRAAELLGAKKYRTYRVYDLPLGADQND"
BASE COUNT          859 a          785 c          837 g          837 t
ORIGIN      
        1 ggtatcggga tggcgatctt gatcggctgc ctgttgacag ctttgccgat aacccgcata
       61 agcgtgtcca tcgagtacca ctccctgttg caggcaaaaa atgtctctcc tgctgctttg
      121 tcagataggg aggccatgcg gatacatcta accagatcat gaacatgaac gatggagaca
      181 taagtggggt cgattccgat cgatggattg ataccagctt tggccagcct gaagaacttg
      241 tacacatcga tatcccgcgg gccatagaca gccgggggtc ttataattgt aacaggaatt
      301 ttgtccttac gcttcaggac ttccttttcg gaagccagct tggactcacc gtaaaaggat
      361 accggagagg gtggatcgga ttccagcacc gcagtcattg acctggaagg gccggcagcg
      421 gcctgggagg agacatgtac aaatcttttt atatcgggag cggtcgccag cacagcttca
      481 accaggttcc gtgtaccatc tctgttgatg ctgaagtaag tttcgcggtt tctggctttg
      541 accagtcctg cgggatgaaa gacataatcg acaccgcgca ccgcctcggg cagagacagg
      601 ggctgcgtaa tttctccgaa aacgagctca aggtcaagat tctcaaggaa ttgcagattg
      661 gaggttttgc gcaccagggc ccggaccctg aatccatgat ttacaagctc ccggcaaagg
      721 tgacttccga tgaagccgtt ggcgccggtc acaagcgcag tctgactgtc agcactctct
      781 ttcatggatt taattttctt gacaaattac ttgtgtcaga ttacccttta gtttttgatt
      841 gaaagaataa ggaaatgcaa ggaagcgaac aagtaaaaat tcgagacata ttcgacaaat
      901 gccgggaatt tacccgggcc actgaggtca aacaggcggg ttattatccc tatttcaatc
      961 cgatctcctc ggctgccgca aataccgtta tgattgacgg caaagagctg attatgatcg
     1021 gctccaataa ttacctgggg cttacaacac atccaaaagt cattgaggcc tccagagagg
     1081 cagtaaagaa atatggtgtc ggatgtaccg gctcgcgctt tttgaatggt accctggaca
     1141 ttcatctgga gctggaagaa gagctggcaa agtttctgag aaaagaggcg gtgctggttt
     1201 tttccaccgg ttttcagacc aacctcggta ctatttcatg tttagtcaat aagaatgacc
     1261 tgatccttgc agaccgttcc gatcatgctt cgattgttga cggctgccgc ctggcattcg
     1321 ggaaaaccct gaaatacaag cataacgaca tcgaggacct ggaacggatt ctggtatccc
     1381 gcaataacgg tgatcagggg attcttctgg tggttgatgg tgtgttttct atggagggcg
     1441 atattgtcaa tcttccgcag cttgtcaaac ttagagagag attcggcttc aggctgatgg
     1501 ttgatgatgc ccattcgctg ggggtattgg gtgaggatgg ttccgggacc gccacccatt
     1561 tcgggttaaa cgatcaggtc gacctgatta tgggtacttt ctcaaagtcg ttcgcttcca
     1621 tcgggggagt gatcgccgct gatgcatatg taataaacta tatcaagcat ttctcgcgtc
     1681 cattgatatt ctcggcagca ttgccgccct cggctgtggc tgccgtgttg gaatgcctga
     1741 agatcataaa ggcggagccg gaacggcgtg aaaagctcct gctgaatggc aagaaaatgc
     1801 gtgatgggct gcgtgccatg ggctactcta tcggtaccac cgaaaccccc atcgtgccgg
     1861 tttatattgg cgaagatatg gcctgcttca aattctggcg caccctttac gatgcaggta
     1921 tttttgccaa tgcaatcatc gcacctgcgg tcaatccagg caatgcgctg atcaggacgt
     1981 catataccgc aacgcacact gacgaacagc tcgattttgt cctggagaca ttcgagaagg
     2041 taggcaaaaa actcggcctc atttagactg acctatgagc ttcgaaaatg tatctattat
     2101 agaagtcaag tcctccgcgg acatggacag gttcattaaa ttcccgttca agctctacgc
     2161 agatgacgag aattgggtgc cgccgctgat ttccgagcgc cgggaattct tcgacaaaag
     2221 taaaaatcca ttcttccgct atgccagggt caaatatttt atggcagaga aggatggcag
     2281 gattgccggg cgcatcgcct ccatcgtcaa tttcaatcac aacgcttttc acgaggacac
     2341 ggtcggtttc ttcggcttct tcgaatgtat tgacgactac caggttgctc aagtgctctt
     2401 gcgtgtggcc ctgattgaac tcaagaaaga gggcatgacc gtgatgcgcg ggccgaccaa
     2461 tttctccacc aattacgaac tgggatttgt catcgatgat ttcgatgctc ccccggttat
     2521 taatatgccc tacaatcccg ggtattatat cagcttcatg gaccgtttcg gattccagaa
     2581 agccaaagac ctctatgcct tcaaaatcac cagggaaaac aagccgcccg agagggttga
     2641 gaagattgtg gaacgaatcc gcaagaagga aaacgttaat gtccgcaatc tcgatctgaa
     2701 ggatttcgat aatgaactca agatcgtcaa caagatttat aataacgcct ggagcagaaa
     2761 ctggggtttt ataccgcttc ccgatgatga atttctgcat attgcccggg atatgagaca
     2821 gattgtcgac ccggatctgg tctttatcgc cgaggttgac ggcaacccga tcggcttctc
     2881 catggctctg cctaacattt atcaggttct accctatgcc aaaggcaggc tctttccttt
     2941 tggagcattg aaactcctgt ggcacactaa aataaagaat aagataaatt ccatgcgagt
     3001 catcacgatg ggtatcgaac atgaatacca gaaacggggt atcgacaaca tcttctacct
     3061 ggatacattc aagcggggct atgataaagg atacgagtgg gccgagattt catgggttct
     3121 ggaggacaat cagctcatga tccgcgccgc tgaactcctg ggagccaaaa aatatcgtac
     3181 ctaccgggta tatgacctgc cgctaggcgc cgaccagaac gattaaaatc ttgtattacc
     3241 acacctgagc gattatctta acttaccgtt aaatttaaat caggggaggg tatcttgaat
     3301 cataacagac gtttgata
//
LOCUS       DSEB01000333            4111 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002055, whole genome shotgun sequence.
ACCESSION   DSEB01000333 DSEB01000000
VERSION     DSEB01000333.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002055"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..756)
                     /locus_tag="ENO07_06030"
     CDS             complement(<1..756)
                     /locus_tag="ENO07_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499956.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HDS01560.1"
                     /translation="MRDVAIIGVGMTKWGEIWEKSLRQMFVETAYEAIADAGIEKLDG
                     MYVGSMSPGLFVGQEHIGALLADYLGQKNMPAGRVESACASGGLAVKCAFLDIASGNS
                     NIILAGGVEKMTDVSGDGATYALATAADQEYEVFHGATFPGLYAMMARRHMHEFGTTR
                     RQLSHVAVKNHENGSKNPLAQYPFKVTLEGVESSTMIADPLTILDCSPITDGAAAVIL
                     ADMETAKKVARNNPLVKIIGIGHASDSIALHDRP"
     gene            complement(769..1815)
                     /locus_tag="ENO07_06035"
     CDS             complement(769..1815)
                     /locus_tag="ENO07_06035"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDS01561.1"
                     /translation="MVGIVGYGAKIPRHRIKVEEIAKVWGADAESYKRGLQLTEKSVP
                     APDVDCVTLSVEATKYALRRAGVTGDQIDALYIGSESHPYAVKPSGTICAEAIGATPH
                     CHNADYEFACKAGSEAMFVCLGLVKSEFCNYAVGIGADTSQGAPGDALEYSAAAGAAS
                     FVMGRENILAECLYTHSWMTDTPDFWRREHQHYPQHGGRFTGEPAYFRTIMGCANALL
                     EKSGMKPADFKYAVFHQPNGKFPMRVGKMLGFEREQLETGWLVPTLGNTYSGASPIGL
                     TAILDVAQPGDKIFMVSYGSGAGSDGFVFKVTDRIKEVQDLAPKTRPQLDENKIYVDY
                     GTYAKYRKKILKND"
     gene            complement(2066..2659)
                     /locus_tag="ENO07_06040"
     CDS             complement(2066..2659)
                     /locus_tag="ENO07_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01562.1"
                     /translation="MGDEGRFDDSKKRFEEGKESTSDEKPEHESAAPKKLQIDPVRFR
                     MFQQQIKDKQNFSMGILAGFIAGSVGAALWGLISYFSSYQIGFMSIGVGFLVGIAIRK
                     FGAGMDVKFGIAGAILSLYGCLAGNIFMVALAVSREYNVPLSEIMSQMNFETMWIFLK
                     AGFEPMDVLFYGIALYFGYKYSFRRIKQEELEKLVIR"
     gene            3515..>4111
                     /locus_tag="ENO07_06045"
     CDS             3515..>4111
                     /locus_tag="ENO07_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01563.1"
                     /translation="MRFVKVILLTLSIIFIWADSGQATQYVNRKLITCEPLIYEVEPI
                     DIYGGSFKLDRSYRNDYGDCTGQIPGPPFVIELPVTIENTYNTDGSVAEFGYFEVDNQ
                     WGGGDPFEIIIYDGAYLKVNGNIVAKGKPGDPIIFNCEGRFYSDFQGGPLPPYSEFTF
                     DFSYCEFYVYPPLNSPTLEMYGGHLKLDNCTFGPTDDQQ"
BASE COUNT         1027 a         1065 c          997 g         1022 t
ORIGIN      
        1 gggacggtca tgcagtgcga tggaatctga ggcgtgacct atgccgatga tctttaccag
       61 gggattattc ctggcaactt tctttgcagt ctccatatcg gccaggataa cagctgccgc
      121 gccatctgtg atcggtgagc aatccagaat ggtcagcgga tcggcgatca tggttgagct
      181 ctccactccc tcgagagtaa ccttgaaggg atactgtgcc aggggattct tggaaccgtt
      241 ttcatgattc tttacggcca catgggagag ctgacggcgg gttgtgccga attcgtgcat
      301 atgccgcctg gccatcatgg cataaagtcc cgggaaggtc gcgccgtgga aaacctcgta
      361 ttcctgatcg gccgcggtag ccagcgcata ggttgcgccg tcgccggaaa catcggtcat
      421 cttctccaca ccgccggcca gaattatatt ggagttgcct gaggcaatgt ccagaaaagc
      481 acatttgaca gcaaggccgc ccgaggcaca ggccgactcg acacgcccgg cgggcatatt
      541 tttctgtcca agataatcgg ccagaagagc gccgatatgt tcctgcccca caaacaggcc
      601 gggagacata ctgcccacat acatgccatc aagtttttct ataccggcat cggctatggc
      661 ttcataagcg gtttcgacaa acatctggcg cagagatttt tcccatatct ctccccattt
      721 tgtcatgccg acgcctatta ttgcaacatc tctcatttta tatcactcct aatcgttttt
      781 caaaattttc ttgcgatact tggcgtaggt gccatagtcg acatagattt tgttttcatc
      841 gagttgagga cgggttttgg gagccaaatc ctgaacctct tttatgcggt cggttacttt
      901 gaacacaaag ccgtccgaac ccgcaccgga accataagag accatgaaaa tcttatcacc
      961 gggctgggcg acatcaagaa ttgcggtcag tcctatggga gatgcaccgg aataagtgtt
     1021 cccaagcgtc gggaccagcc atccggtctc gagctgttcg cgctcgaagc ccagcatctt
     1081 gcccaccctc attgggaact tgccgttggg ctgatggaaa accgcatact tgaaatcggc
     1141 cggtttcata cccgactttt caaggagcgc attggcgcaa cccataatgg tccggaaata
     1201 tgccggctca ccggtaaacc ttccaccatg ctgaggataa tgctgatgct cgcggcgcca
     1261 gaagtcggga gtatcggtca tccatgaatg cgtgtaaaga cattcggcca gtatattttc
     1321 tcttcccatc acaaatgagg ccgcaccggc ggcggcggaa tattcgagcg catcaccggg
     1381 agcgccctga gatgtatccg cgccgatacc aacagcataa ttgcagaact ctgattttac
     1441 caaacccagg cagacgaaca tcgcctccga gccggctttg caggcaaact cgtagtccgc
     1501 attatgacaa tggggtgtgg cgccgatcgc ctcggcacag atcgtgcccg agggcttaac
     1561 cgcataagga tgcgattcgg aacctatata gagcgcatct atctgatccc cggtaactcc
     1621 cgcgcgcctg agagcatact tggtggcctc aaccgagagt gtaacgcagt ccacatcggg
     1681 agcggggact gatttctctg ttagttgaag accgcgcttg tatgattcag cgtcggcgcc
     1741 ccagactttg gcaatctctt caactttaat gcgatgccgg ggtattttgg caccatatcc
     1801 aacgattcct accataaaac ctctctatct ttatttttta catttttctt ctataaccga
     1861 gtacaaggca acgttttccg gcagtggacc ggccacatta gaatttcaat agatagtgaa
     1921 aaaatggccg aaagtcaagc gattttggcc gtggagcctc gctgtttgca attattaata
     1981 atgtttttgc ggaccagaag tttccataaa gcagagaaaa atgattgcgc ttaatcatca
     2041 ggccctgtgc tggtgcccgg gtcatttatc ggatcacgag tttctcgagc tcctcctgtt
     2101 ttatccggcg aaaagaatac ttgtaaccga agtaaagtgc gatcccgtag aaaaggacat
     2161 ccataggttc aaaaccggcc ttcaggaata tccacatggt ctcgaaattc atctgcgaca
     2221 taatctcgga aagcgggaca ttatattccc tggagaccgc cagagctacc atgaatatat
     2281 tgcccgcgag acagccatac aaagaaagta tcgcccccgc aattccgaac ttgacatcca
     2341 tacctgcccc gaatttacgt attgctatcc cgacaaggaa gcccacaccg atcgacatga
     2401 agccgatctg ataactgctg aaatagctta tcagccccca cagtgcggcg cccacagagc
     2461 ccgcaataaa tccggccagg attcccatgc tgaagttctg tttatctttg atctgctgct
     2521 ggaacattct gaaccgtacc ggatcgatct gcaacttttt gggagctgcg ctctcgtgct
     2581 ccggcttctc gtcagatgtt gactctttac cttcctcaaa tctcttcttg gaatcgtcaa
     2641 atcttccttc atcccccata atagctcctt gtatatattc cgatccgccc gttcaggggc
     2701 tttaatatta agcggtggta cggatttata atatgctgtt attgaatagt agcaaggaga
     2761 ttttgtgcag aaattgacag cgggggtcct cgtgccagtt ctcgggttca accagtccgc
     2821 aaagctgatg gagggaatat tctgcggcag gatttttggc cacctcaaaa gtccaggcct
     2881 ccctctcttt tgcagcttgc attcttcgat ctatcaacac tcaggaaagc cagatatgga
     2941 ttgcagaaga acaaaacaat acacattacg aacgcggata aaagctcctt cagatttgtg
     3001 agaggtttga tcttaaaagc actgcgctcc gcctcgcgag taaagttttt cgcccgtcca
     3061 ggaaagaatc attctgaagc cagtatctta tgctgtatgg acttgtcaat gtcggacttt
     3121 ttccaggcat cattccagtc gggcacggtg ggaagtgtgc gccttccact tcgcgtcagg
     3181 tgcgacacgg tgcgcggttg aaaaagccgg agcagagatg atgattgcag gtcacaccgc
     3241 tctgatcgac agtgttgcag aatgcgacaa ccataatgcg acatattcag gacaatccgg
     3301 cagcagatca aataatcagc ctgcggagat aataagattg acagcggatt acaactcaaa
     3361 tattttgagc ttgtgcgggg tgactcagct gagcaatgga caatttgatt atccgataga
     3421 tcgggcgcca gagactacac cgctgctgaa agccgcgcac aaagcatgac caattgctgg
     3481 cgtgtgatct acaccgggaa gggagacatc agccatgcgt ttcgtcaaag taatcctgct
     3541 gacattgagc atcatattca tctgggccga ttcaggccag gccactcagt acgttaatcg
     3601 aaagttgatt acgtgtgagc cgctcattta cgaagtcgaa ccgatcgaca tatacggagg
     3661 gagcttcaag ctcgaccgca gttaccgcaa tgattacggt gattgcaccg gtcagatacc
     3721 cggtccgcca ttcgtgatag aacttccggt gaccattgag aacacataca atacggacgg
     3781 atctgttgcg gaattcggct atttcgaggt cgacaatcag tggggagggg gcgatccgtt
     3841 tgagataatt atctacgatg gcgcttatct caaggtcaac ggaaatattg tcgccaaggg
     3901 taaacccggt gacccgatca ttttcaattg tgagggccgt ttctatagcg attttcaggg
     3961 agggccgctt cctccctaca gcgagttcac ttttgatttc tcatattgcg aattctatgt
     4021 ttaccctcca ttaaattctc caacacttga aatgtatggt ggacatctta aactcgacaa
     4081 ctgcaccttc ggacccaccg atgatcaaca a
//
LOCUS       DSEB01000334            2006 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1010003, whole genome shotgun sequence.
ACCESSION   DSEB01000334 DSEB01000000
VERSION     DSEB01000334.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1010003"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..725)
                     /locus_tag="ENO07_06050"
     CDS             complement(<1..725)
                     /locus_tag="ENO07_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDS01564.1"
                     /translation="MAEVIKNKEIADIGLDYATRYGFRDPEEYFHKGARGVNHEVVEM
                     ISQMKGEPRWMADFRHKALDIFLSKPMPTWGNTKLLNSIDFDNIYYYIKPIEKQGKSW
                     DEVPESIKNTFDKLGIPEAERKFLAGVSAQYESEVVYHSMQKNLEDQGVIFKDMDTGL
                     REHEDIVKKYFSTVIPPNDNKFAALNSAVWSGGSFIYVPPGVDVKIPLQAYFRINAEN
                     MGQFERTLIIADKGSRVHYIEGCT"
     gene            complement(727..1500)
                     /gene="sufC"
                     /locus_tag="ENO07_06055"
     CDS             complement(727..1500)
                     /gene="sufC"
                     /locus_tag="ENO07_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDS01565.1"
                     /translation="MSDKKTIFSFSDIHVEVEGKEVVKGVSLDIKAGEIHAVMGPNGS
                     GKSSLANALAGHPSYEITGGKAIIDGQDLLEMDATERALAGLFLAFQYPLAIPGVTVA
                     NFLRAAVKARHAGDEEALRSFRKDLKEKFKLLEVDQSFATRYVNDGFSGGEKKRLEIL
                     QMAMLDPKIGILDETDSGLDIDALKVVSKGINSVASDKNGMLLVTHYQRILDYIKPDK
                     VHVMMNGKFVLEGGPELALELEEKGYDWLKAENQQAVEV"
     gene            complement(1517..>2006)
                     /locus_tag="ENO07_06060"
     CDS             complement(1517..>2006)
                     /locus_tag="ENO07_06060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDS01566.1"
                     /translation="NYKGLFGNKIVKVSALEEYGLRCMLQLARKNGGPMTLPEISKAE
                     GVSLSYAGKLLMILKKAGLVKAVRGRQGGYALVKPAEEYDLKEIFDALGEPLYNPKHC
                     RKFTGDLVKCAHSKDCTVRDIWKEFSKIIDGVLGNMTLADVASGSYDFGNMTSNIQVG
                     IK"
BASE COUNT          494 a          566 c          402 g          544 t
ORIGIN      
        1 gtacaaccct ctatataatg tacacgtgag cctttatcgg cgatgatcag ggttcgctcg
       61 aactggccca tattctcggc attgatccgg aagtaggcct gaagaggaat cttgacatcc
      121 acccccggcg gaacatagat aaatgaaccg cccgaccaaa ccgctgaatt cagggccgca
      181 aacttgttgt cgttgggcgg gataacggtc gagaaatact tcttgacgat atcctcatgc
      241 tccctgagac cagtatccat atctttgaat ataacaccct gatcctcgag attcttctgc
      301 atcgaatgat atacgacctc ggattcatac tgggccgata cacctgccag gaacttgcgt
      361 tcagcctcgg gaataccaag tttatcgaat gtgttcttga tagattccgg gacctcgtcc
      421 catgatttgc cctgcttttc aatcggtttt atataatagt atatattatc aaaatctata
      481 ctgttcagga gcttggtatt tccccaggtc ggcatcggct tggacagaaa gatgtccagc
      541 gccttatgac ggaaatccgc catccagcgg ggctcgccct tcatctggga gatcatctcc
      601 accacttcat gatttacccc cctggcgccc ttatggaaat attcctccgg atcacgaaac
      661 ccataccttg tggcgtaatc aagtcctata tctgcaatct ctttattttt aataacttcg
      721 gccatattac acctcgactg cctgctgatt ctctgctttt agccaatcgt aacccttctc
      781 ctcgagctcg agggcaagct ccggtccccc ctcaaggacg aatttgccat tcatcattac
      841 atgaactttg tcaggcttga tgtaatccaa aattctctga taatgagtca ccagaagcat
      901 gccgtttttg tccgaggcta cagaattgat gcccttagaa accaccttca atgcatcgat
      961 atccagaccg gaatcggttt catccagaat gccgatctta ggatcaagca tggccatctg
     1021 cagaatctcc agacgctttt tctcaccgcc cgagaaaccg tcattaacat aacgtgtggc
     1081 aaacgattga tctacttcca gtagcttaaa tttttcctta aggtcctttc tgaacgacct
     1141 caaggcctcc tcatcacctg catgcctggc cttgacagcc gcccgcagga aattggcaac
     1201 agtcacaccg ggaattgcca gcgggtactg aaaagccaga aataatcccg ccaatgcacg
     1261 ctcggtagca tccatttcaa gcaaatcctg cccgtcgatg atggccttcc cgcccgtgat
     1321 ctcatagctg ggatgcccgg ctaatgcgtt ggcaaggctg gacttacccg agccgttggg
     1381 acccattaca gcatgaattt cgcccgcctt tatatccagc gaaacaccct tgacaacctc
     1441 cttgccctct acctcgacat ggatgtcgga gaatgaaaat attgtctttt tatcgctcat
     1501 gattacctcg tcttattcat tttattccaa cttgtatatt cgatgtcata tttccgaaat
     1561 cataactgcc cgaggccaca tcggccagag tcatattccc cagaaccccg tcaataatct
     1621 tgctgaattc tttccagata tcacgcacag tgcaatcttt tgaatgcgca cattttacaa
     1681 ggtcgccggt gaatttacgg cagtgcttgg gattatataa gggctccccc agggcatcaa
     1741 aaatctcctt aagatcatat tcctcagccg gtttgaccag ggcatatccg ccctgacggc
     1801 cccttactgc cttgaccaga ccggcctttt tcaggatcat caggagcttc cccgcatacg
     1861 agagcgaaac accctcggct tttgatatct ccggcaatgt catcggcccg ccattttttc
     1921 tggcaagctg gagcatgcac cggaggccgt attcttctaa cgccgatact ttcacaatct
     1981 tatttccaaa taatccttta taatta
//
LOCUS       DSEB01000335            2454 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1006434, whole genome shotgun sequence.
ACCESSION   DSEB01000335 DSEB01000000
VERSION     DSEB01000335.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1006434"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            580..1959
                     /locus_tag="ENO07_06065"
     CDS             580..1959
                     /locus_tag="ENO07_06065"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00691.18,HMM:PF13505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01567.1"
                     /translation="MENTRTNFRIIHLALSILTLVLWAGTLHADFSLERKFSLFIIAG
                     ARQPAFNPGWQPWVISPAFSMGAGIGLNERLMLTGEFEFGKVYNDTVSNSIYKIGREN
                     ADRYWQINTIKTRLKYHFTGESKFVPYLASGIGLSFWSIKFTADDEAVQVRDGNGDIV
                     DYSATEFLLSGGLGCEWFVRENISVSVDACLNYLTGIGADFSDSVQDFRSRAYGDLKF
                     GVSLYFPLEKGEVSISYEEPEGSDTAEEQLLEEDSDLDGVPDAVDLCPDTPPEAGELV
                     DEHGCPLDSDEDGLPDYRDSCPDIFAEITLDSTGCPPDSDRDNVPDQIDRCSNTPPGY
                     PVDDSGCAILDSIFFSRTMHVDFSESGRGIDFRSLRTLDNIAMAMRDFPDVDVVVKAY
                     TDNTLSLEESKKRAELEAIKIKSYLVERRVEEGRIRAVGMGATDFIDTNTSAEGRANN
                     RRIVIEFRY"
BASE COUNT          591 a          579 c          654 g          630 t
ORIGIN      
        1 ggctgcaaat gtttgggatc agggcaataa cagttatctg cagagtattg atgatagtgg
       61 ctattgccag tggtcgaagt ttcatgattg ggaaatgacc tggaatccgg aagaactggc
      121 ctcgtacctg agggattacc tgcataaaaa tagaggcttt aatgatgatg cgcttgtaat
      181 aacagatatc gaggtggttg agcgctttcc gtcaggcagg atatcatctt tgaaagtcat
      241 gacagatcat ggcgagttcc tgttttttaa ggaccagatt cgatgggcat tccggcggcc
      301 ggaaaggccc gatttaatcc tgccttcatc gaatttcgaa atccggctct ggcaggattc
      361 cgttggaagt ctgtccgaaa tcatggccgc gggcaggggc tacggtcatg gcgtggggat
      421 gtgtcagact ggcgccatag gacgcgcgcg ggcggggcaa aactacgagc agatactcag
      481 aacttattac tccggagccg aactgaaaaa agcgtattga tattccgggc gtcagaaaag
      541 acttgccaac gagggtaaaa ctcatcatta ttgacgctta tggagaacac caggacaaat
      601 ttcagaatta tccatcttgc gctgtccatc ctcactctgg ttctgtgggc gggaactctc
      661 catgctgatt tcagcctgga gaggaagttc tcgcttttta taattgccgg ggctcgtcaa
      721 cccgcattca atcccggttg gcagccctgg gtcatatcac ccgctttctc aatgggcgca
      781 ggtattggcc ttaatgagag attgatgtta acgggagaat ttgagttcgg aaaagtatac
      841 aatgataccg tttcaaacag catatataaa atcgggcgcg aaaatgccga ccgttactgg
      901 cagatcaaca ccataaaaac caggctgaaa tatcatttta ccggagaaag taaatttgtg
      961 ccatatcttg catccggtat aggcctgtca ttctggtcaa ttaagttcac ggctgacgat
     1021 gaagcggtcc aggttcgtga tggcaatggt gatattgtgg attacagtgc cacagagttc
     1081 ctgctcagcg gaggccttgg atgtgaatgg tttgtgcgtg aaaatatttc tgtcagcgtg
     1141 gatgcctgcc tgaattatct caccggcatt ggcgcagact tctccgacag tgtacaggat
     1201 ttccgttcac gggcatacgg ggacctgaag tttggcgtct ccctatattt ccccctggag
     1261 aaaggcgaag ttagtatctc atacgaggag cccgaaggaa gcgatacagc tgaggagcag
     1321 cttttagaag aagatagcga ccttgacgga gtcccggatg ctgttgatct atgccctgat
     1381 actcctcccg aggcaggcga gttggttgac gaacatggct gtccgctgga cagtgacgag
     1441 gacgggctgc ccgactatcg tgacagttgt ccggatatat ttgccgaaat tacactggat
     1501 tcgaccggct gcccgcccga cagtgaccgt gacaatgtgc ccgatcaaat tgacaggtgc
     1561 agcaacaccc cgccggggta tccggtggat gattccggct gcgcaattct ggattcgatt
     1621 ttcttctccc gtacaatgca tgtggacttt tccgagtcgg gcagaggcat agatttcaga
     1681 tccctgcgca cacttgacaa tatcgccatg gccatgcgtg attttcccga tgtcgatgtt
     1741 gttgtaaagg cctacaccga taacacgctc agcctggaag aaagtaagaa gagggctgaa
     1801 cttgaggcga tcaagatcaa aagttacctg gtggaaagga gagtagaaga ggggcgaatc
     1861 agggccgttg gcatgggtgc taccgatttt atcgatacca atacctccgc cgagggccgg
     1921 gccaataacc gtcgcatagt gatagaattc agatactgat tcatttcttg cccttttcca
     1981 aaagctcttc ggcctgtttc aatgacagct ccgtcacttt ttcacccgaa atcatccggc
     2041 ccagttcttc cttgcgctgc tttaaggtca atttcctgat ctgtgtgatc atgcggccat
     2101 tggatttttc tttgaatacg agaaagtggt tctcgccgaa agccgctatt tgctgtaaat
     2161 gtgttatgac caggacttgg tggcccctgg aaagattctg aagacttctt ccgactgcgg
     2221 aggcggtttt gcccccgatt ccggaatcga tctcatcgaa gatcaggctt cggcccttgt
     2281 ttttgtccga cacgattgtt ttgatcgcca gcagcacgcg ggaaagctcc cctcccgaag
     2341 caattttagc cagcggtttt ttctcctccc cgggattggg cgagaagaga aattccacaa
     2401 tgtcgtatcc atccgggccg aggctgagaa tcgtgtcccc tttctcatat tcac
//
LOCUS       DSEB01000336            1934 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1010874, whole genome shotgun sequence.
ACCESSION   DSEB01000336 DSEB01000000
VERSION     DSEB01000336.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1010874"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(1..810)
                     /locus_tag="ENO07_06070"
     CDS             complement(1..810)
                     /locus_tag="ENO07_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01568.1"
                     /translation="MKAPGKTFLLWFVAVILTLSSAVYQRLTGPTHPVRGSVEVDGTE
                     ISYELLRSHYTSADAIMKIQLHDAEVEGEIRWRRYKSHDPWFTDKLPQRADTLIITIP
                     KQPSAGKVMYSLSLIDKSGNRYELLREPVIIRFKDPVPIYILIPHIIAMFGSMLLSTR
                     TGLEAIFRNRNTYRLTLWTIGFLFIGGLILGPIVQKFAFGAFWTGWPLGTDLTDTKTA
                     VALLFWLIAMWRLRRRSGGRVRTIIASVVTLIIFLIPHSLLGSEIDYTEIE"
     gene            1010..>1934
                     /locus_tag="ENO07_06075"
     CDS             1010..>1934
                     /locus_tag="ENO07_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDS01569.1"
                     /translation="MSDANNKDETKEIILDSIADGVFTIDKDWKITSFNRAAEQITSI
                     PREEAIGQRCCDVFRASICESSCALRETFRTGEPVVNKAVFIIDASGRKIPISISAAV
                     LRNKDGEIIGGVETFRDLSAIEELKRELEKKYSFQDIISKNSAMLKIFDILPSIAESD
                     STVLIEGDSGTGKELIARAIHNLSNRRDNPIVTINCGALPDTLLESELFGHKAGAFTD
                     ARRDKPGRFALAEGGTIFLDEIADTSPSLQVKLLRVIQEKVYEPLGGTESVEANVRII
                     TASNRNLQKLVKAGRFREDLFYRINVITVELP"
BASE COUNT          521 a          476 c          481 g          456 t
ORIGIN      
        1 ctattctatc tcggtgtaat ctatctcaga gcccagcagg ctatgcggta tcaggaagat
       61 tatcaatgta acgaccgatg ctataatcgt ccggacacgt ccgccgctgc gcctgcgcag
      121 gcgccacatt gcaatcagcc agaataaaag ggcaacggct gttttggtgt cggtcaaatc
      181 ggttcccagg ggccatccgg tccagaatgc tccgaaggca aacttctgca ctatcggccc
      241 cagaatcagc ccgccgataa acagaaaacc gatcgtccag agggtcagcc tgtatgtatt
      301 tcgattgcgg aatattgctt ctaatcccgt acgggtggac agaagcatgg atccgaacat
      361 ggctataata tgcggtatca agatatagat cggcaccgga tctttgaagc ggattattac
      421 cggctcccgt agaagttcat accgatttcc ggatttatcg atcaggctca gactgtacat
      481 tacctttccg gctgagggct gcttgggaat cgttatgatt agagtatcgg ctctttgggg
      541 caacttatcc gtgaaccagg gatcatgact tttatacctg cgccatctga tttcaccctc
      601 tacctccgca tcatgaagct gaatcttcat gatggcgtcg gcggaggtat agtgcgacct
      661 gagcagctcg taactgattt cagtaccatc aacctcgaca gagcctctga ccgggtgggt
      721 cggtcccgtc aggcgctggt atacagcaga agaaagagta aggattacag caacaaacca
      781 gagaagaaat gttttgccgg gtgctttcac ttttttcgaa tccaatcgtt cgtcctgtct
      841 gtttttcaag agagccaaga tacaaaaacc ggttataatg gcaagcggga ttttaagcgg
      901 atgtaatctt taatcccgaa gtaattagat taatatcatt tgacacccat ctttaactcg
      961 tatatatatg ttgaaatata acccattata attaagcata ttgggaacta tgagcgacgc
     1021 gaataataaa gacgaaacca aagagataat tttagattca attgccgacg gtgttttcac
     1081 gatcgacaag gactggaaga taacctcctt caaccgggct gccgagcaga ttacctctat
     1141 cccgcgcgag gaagccatcg gccagagatg ctgtgatgtc ttccgcgcct cgatctgcga
     1201 atccagctgc gcactgaggg aaaccttcag gaccggtgaa ccggttgtaa ataaggctgt
     1261 cttcattatt gatgcctcgg gacggaaaat cccgatcagc atctcggctg ccgtcctgcg
     1321 caataaggat ggagaaataa tcggtggtgt ggaaacattc cgtgacctca gcgcaatcga
     1381 ggagctcaaa cgcgaactgg aaaagaaata ctcgtttcag gatatcatca gcaaaaatag
     1441 cgcgatgctc aagatcttcg atatcctgcc cagtattgcc gaatccgaca gcacggtcct
     1501 tatagagggc gactcgggca ccggtaagga gctgatcgca cgcgcaatac ataatctatc
     1561 aaatcgcaga gacaatccga tcgtcacaat aaactgcgga gcattgccgg atacgctgct
     1621 tgagtcagaa cttttcgggc ataaagccgg agcattcaca gatgcccgca gagacaaacc
     1681 gggacgcttt gcgctggcgg aaggcgggac catattcctg gatgaaatcg ccgacacctc
     1741 accctcgctg caagttaagc tgctgcgggt gattcaggaa aaagtatatg agccgctggg
     1801 cggcactgag agtgtcgagg ccaatgtccg cattataacc gcttccaata gaaatctgca
     1861 gaagctcgtt aaggccggcc gcttcaggga ggatctgttt taccgtatca atgtgataac
     1921 cgtcgagctt cccc
//
LOCUS       DSEB01000337            2118 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1008903, whole genome shotgun sequence.
ACCESSION   DSEB01000337 DSEB01000000
VERSION     DSEB01000337.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1008903"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..714
                     /locus_tag="ENO07_06080"
     CDS             <1..714
                     /locus_tag="ENO07_06080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HDS01570.1"
                     /translation="TLMIEAWLKSNVDLSASSMGFVMNRWLFDIDTAYWTGNVILTIP
                     QMQWRADTLIMGGIAFAPQIMFVASEEYVHVASAVLIYNPDSAGVLVDNTVEFYMDSS
                     YFPPAGDFIVTNVGGVSVVPIIENDTVFLHRLEVTDINPGVIPAKFELGQNYPNPFNP
                     TTNIEFAVPSRTHVKLNVFNIAGQKVRTLVNEELEAGWKLVTWDGKTDSGDEVGSGVY
                     LYRVEAGDFVESKKMMLVK"
     gene            929..>2118
                     /locus_tag="ENO07_06085"
     CDS             929..>2118
                     /locus_tag="ENO07_06085"
                     /inference="COORDINATES: protein motif:HMM:PF02449.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01571.1"
                     /translation="MIALSDHKIIIGKEDYLPFSAEIHYFRVPKRYWSICFERIKRAG
                     FKIISTVIPWNHHEDKNRDFDFSGFADQSKDLIVFIELAREFGFKMIVRPGPFINSQV
                     RRNGLPTFLDKYPDIFALNSEGKVVKAVSQDDVEVCGFPSLMNPRYLNFVKHYLNGLT
                     EIIKNYIYPRGPIFMIELGSDNFFGGNFDPKSADFNEYNMRELYPEFLESLYPDIKSF
                     NKAYGLKIKDFSQLKELEEINQIQDHSLISKMDWLRFKRYLQTSYVNTLRDFYISFGC
                     NPLFLGTVSFRSNVQPPMNDFDPPEEDEPKILGATLDWNDTSSDILQKARYLAANSDF
                     PFATEISVGMSSSDPQKSKNFFPISDRATRYILTLALAGGIKGFNANMFVERDHWYDA
                     ALAN"
BASE COUNT          553 a          483 c          503 g          579 t
ORIGIN      
        1 accctgatga ttgaagcctg gttgaaatcc aatgtcgacc tgagcgcttc gtcgatgggc
       61 tttgtgatga acaggtggct atttgacatc gacacagctt actggacagg aaatgtgatc
      121 ctgactatac cacagatgca gtggagagca gacacactca taatgggagg aattgccttt
      181 gccccgcaaa ttatgtttgt tgcgagcgaa gaatatgtcc atgttgcctc cgccgtttta
      241 atttataacc ccgattcggc cggtgttctg gtagataata cagtggaatt ctatatggat
      301 tcctcatatt ttccacctgc cggcgacttt atcgtgacca atgtcggcgg cgtttcagta
      361 gttcctatta tcgagaatga tacagttttc cttcacagat tagaggtaac cgacatcaat
      421 ccgggtgtta ttccggccaa atttgaactg ggccagaact atcccaatcc attcaacccg
      481 accacaaata ttgaatttgc tgtgccctcc agaacacacg tgaagctcaa tgtcttcaat
      541 attgccggcc agaaggtgag aactctggtc aacgaagagc tcgaagccgg ctggaagctg
      601 gtgacctggg acggtaaaac ggattccggt gacgaggttg gaagcggcgt atatctctac
      661 agggtagaag cgggcgactt tgtcgagagc aagaaaatga tgctcgttaa gtaagaagat
      721 agcgttttat aagatataag gctgtgccat attgacatgc aatgcatgcc aatgcggcac
      781 agccgttttt tttgtcagca cctgccctcc atccccataa gttactatat ttcagatagt
      841 tacattcatg gtgttgccga tttgcatcga aatatttaat ttttattgac tttgagggcc
      901 gatttagata aaataggctc taattattat gatagcctta tcagatcaca aaataatcat
      961 cggaaaggaa gactatcttc cgttttctgc cgagattcat tattttcgtg tgcccaaaag
     1021 atattggtcg atttgcttcg agcgcatcaa acgggccggt ttcaaaataa tctctaccgt
     1081 aattccctgg aatcatcatg aagataaaaa tcgggacttc gatttctcgg gatttgccga
     1141 tcaatccaag gatctgattg ttttcattga attggcgcgc gaattcggat tcaaaatgat
     1201 agtgcgtccc gggccgttca taaattcaca ggtcaggcgc aacggcttgc cgacgtttct
     1261 ggacaagtat cctgacatat ttgctttgaa ttcagagggt aaggtcgtga aggctgtatc
     1321 gcaggatgat gtcgaggtct gcggctttcc ttcactgatg aatccgcgct atctgaattt
     1381 tgtgaagcat tacctgaatg gcctgaccga gatcataaag aattacatct atccacgcgg
     1441 tccgattttc atgatagagt tgggcagcga caacttcttc ggcggcaatt ttgatcccaa
     1501 aagcgccgac tttaacgaat acaatatgag ggagctttat ccggaatttc tggagagcct
     1561 gtatccggac atcaagtcat tcaataaggc ctacggcttg aaaatcaagg acttcagcca
     1621 gttgaaggag ctggaggaga tcaatcaaat tcaggatcac agcctgattt cgaagatgga
     1681 ttggctgcgc ttcaagcgct acctgcagac cagttatgtg aatactctca gggatttcta
     1741 tatctcattc ggatgcaatc cattattcct cggcactgtc agcttcaggt ccaatgtgca
     1801 gcctcccatg aacgattttg atcctcccga ggaggatgaa cccaagattc tgggagccac
     1861 cctggactgg aacgatacct cctcggatat cctgcagaaa gctcgttatc tggccgcaaa
     1921 cagcgatttc ccgtttgcca ccgagatttc ggtgggcatg tcttcctccg atccgcagaa
     1981 atcgaagaat ttcttcccca tatccgaccg cgccacgcgt tatattctga ctctggcgct
     2041 ggcaggggga attaaagggt tcaacgccaa tatgtttgtt gagcgggacc actggtatga
     2101 tgccgccctg gcaaatga
//
LOCUS       DSEB01000338            2153 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1008613, whole genome shotgun sequence.
ACCESSION   DSEB01000338 DSEB01000000
VERSION     DSEB01000338.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1008613"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..>2153
                     /locus_tag="ENO07_06090"
     CDS             <1..>2153
                     /locus_tag="ENO07_06090"
                     /inference="COORDINATES: protein motif:HMM:PF00128.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDS01572.1"
                     /translation="PNVRKEMFRVIDFWLKMGVDGVRLDAVPYLYEREDTNCENLPET
                     HEFLKELRSHIDSKFEDRMLLAEANQWPEDAAAYFGEGDECNMAFHFPVMPRMFMAIQ
                     MEDSFPITDILKETPEIPENCQWAMFLRNHDELTLEMVTDEERDYMYRVYARDPRTKI
                     NLGIRRRLAPLLSNNRRKIELMNILLMSMPGTPVIYYGDEISMGDNYYLGDRNGVRTP
                     MQWSPDRNAGFSTANPQRLFLPVIIDPEHHYETLNVENQERNLSSLLWWMRRVIAMRK
                     QYKAFSRGSFELIPSDNPKILSFARKFEDEAIIVIVNLSRFSQVVNLDLCEYQNCIPE
                     EVFSQNLFPAIKQEEYVITLGPHNHYWFLLHPEAEFEKEIEFDGDPVVSVRGEWLNLI
                     RGRQREKFEKAILPKYVKKARWFGAKTKKVRRVKIEEEGFTALTPTSSVVLIIRVTYT
                     DGSQDLFVLPLSFVTEKKAEELIADFPQSLVMRLRAEGEEGIVCDATYDERFHKALLG
                     SIWRRKKIKGRNSDFVGRPAGNLRKILADTGVEIDSWPMKAEQSNNSIVYDNRVVLKI
                     YRHMQEGMNPDYEITRYLTEKARFKNIPAYTGSIEYIDKSGDNYIIAMAQEFVENSGD
                     AWSYSSDVVSKYFDRALTKGKPAYEIMEEYPAFTEVNEAEIPHIMHELIGGLFLEMMS
                     ILGRRTGEMHLALIGEQDDKDFRPESFSTLYQRAL"
BASE COUNT          593 a          475 c          568 g          517 t
ORIGIN      
        1 atcccaatgt caggaaagag atgtttcggg tgattgactt ctggctgaaa atgggtgtgg
       61 acggtgtgcg cctggatgct gtaccctatc tttatgagcg cgaggataca aattgcgaga
      121 acctgccgga gacgcatgaa tttttgaaag aactgcgctc ccatatagac agcaaatttg
      181 aggacaggat gctgctggcc gaggccaacc agtggccaga ggatgcggct gcctatttcg
      241 gcgaagggga tgaatgtaat atggccttcc attttccggt aatgccgagg atgtttatgg
      301 caattcaaat ggaagacagt tttccgatta cggatatttt gaaggagact ccggaaattc
      361 cggagaactg ccagtgggct atgttcctca ggaaccatga cgagctgacg cttgagatgg
      421 taactgacga ggaacgggac tacatgtacc gtgtctatgc ccgcgatccg cgcaccaaga
      481 tcaacctggg aatacgcaga cgtctggccc cgcttttgag caataaccgc aggaaaattg
      541 aactgatgaa tatcctgctc atgtcaatgc cgggcacccc ggtgatctat tatggagatg
      601 agatttcgat gggtgataat tattatctgg gcgaccgtaa cggtgtacgc actcccatgc
      661 agtggagccc ggatagaaat gccggatttt ctaccgccaa tccacagaga ctgttcctgc
      721 ctgttataat cgacccggaa catcattacg aaacgctcaa tgtcgaaaat caggaacgca
      781 atctctcctc ccttttgtgg tggatgaggc gtgtaatcgc aatgcgcaag caatacaaag
      841 ccttcagccg cggcagtttt gaactgatac cctctgacaa tcctaaaata ctttcatttg
      901 ccagaaaatt cgaggacgaa gcgattattg taattgtcaa tctgtcgcgc ttctcacagg
      961 ttgttaatct tgatctatgt gaatatcaaa actgcattcc ggaggaggtg ttcagccaga
     1021 atctgtttcc cgccatcaag caggaggaat atgtgatcac attgggaccg cacaatcatt
     1081 actggttcct gttacatccc gaagctgaat ttgaaaagga gattgaattt gatggagatc
     1141 cggtcgtcag tgttcgggga gaatggttga atctcatcag ggggcgccag cgcgaaaaat
     1201 ttgaaaaggc gattcttccc aaatacgtaa aaaaagcacg ctggttcggc gccaagacta
     1261 aaaaagtgcg ccgggtgaag atcgaagagg aaggcttcac cgccctcacg ccaacttctt
     1321 ccgtggtcct gatcatacgt gtgacctaca ccgacggcag ccaggacctt ttcgtgctgc
     1381 ccctgtcatt tgtgacggaa aagaaagctg aggagcttat cgcagatttt ccccaaagcc
     1441 tggtcatgcg tctgcgggcg gagggtgaag aaggcatagt ctgtgacgcc acctatgacg
     1501 aaagattcca taaggcgctt ttgggctcga tctggagaag aaaaaagatt aagggccgga
     1561 attccgattt cgtcgggcga ccggcaggaa atttaagaaa gatactcgcc gatacgggag
     1621 tggaaattga ttcatggccc atgaaagccg aacagagcaa caactcgatc gtgtatgata
     1681 atagggtcgt cttaaaaata taccggcaca tgcaggaagg catgaatcct gattatgaaa
     1741 taacccgcta tctgaccgag aaggccaggt ttaagaatat acccgcatat accggatcga
     1801 ttgaatatat agataaaagt ggcgataatt atatcatagc catggcccag gaatttgtag
     1861 aaaattcagg agatgcctgg agttattctt ctgatgttgt ctccaagtat ttcgatcgag
     1921 cgctgaccaa aggtaaaccg gcttacgaga ttatggagga atatccggcc tttaccgagg
     1981 tcaatgaagc cgagattccg catatcatgc acgagctgat tggcggcctg tttctggaga
     2041 tgatgtcgat tcttggcagg cgtacaggcg agatgcatct ggctttgata ggagaacagg
     2101 acgataagga tttcaggccg gagagttttt ccacccttta ccagagagca ctc
//
LOCUS       DSEB01000339            2181 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1008373, whole genome shotgun sequence.
ACCESSION   DSEB01000339 DSEB01000000
VERSION     DSEB01000339.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1008373"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..360
                     /locus_tag="ENO07_06095"
     CDS             <1..360
                     /locus_tag="ENO07_06095"
                     /inference="COORDINATES: protein motif:HMM:PF00027.27"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HDS01573.1"
                     /translation="TEQLASLASIAEEVEYIKGDVIYKEHDPSDALYLVLEGKVKLHR
                     GDQEISTAEKDDAFGTWALFDEEPRVVTATAAADCRLLRVDREDFIDLLADHVQIAQG
                     VIKTVVKRLRNLVERVA"
     gene            389..1972
                     /locus_tag="ENO07_06100"
     CDS             389..1972
                     /locus_tag="ENO07_06100"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00512.23,HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HDS01574.1"
                     /translation="MVKADNSDLKSLRESEEKYRKMIEMASDAIFSIEPADGTILEAN
                     LRAEKLTGFSVDELVGKKVWELHPESEWEESRRLFNQVVESGKGYTGELHFLRKDKTL
                     LDIEISAGVINYGDKKIIQRICRDITSRVLLQRENRRLREYYEYILDLMPVGLGVRKN
                     INGEPKVEFENKKLKEIFDRARDEGCPCGWEQSIDDQRVESEQILSEDGVYVEERIFP
                     NNRVYQFTTGYFRDIKHNWRELNIVQDVTTRYRLEQELKKANEELEQKVEERTRELRQ
                     KQTQLVQAEKMAALGNLVAGVAHEINTPLGALNSNNDLFIRSIEKLKAMVSDSAAPTE
                     FRESKQLGKLLENMEDLTRINKTASSRIINIVSSLRKFARLDQAEMDTVNIHDGIENT
                     LTLVHHQLKDRVEVEKDYGDLPKISCYPNQLNQVFMNILVNASQAIEDKGKIIIRTRR
                     QGGDAVIEFSDTGRGIPEDKLERVFDPGYTTKGAGVGTGLGLSIVHQITEDHKGRIEV
                     ESRVNQGTTFRIIIPIRGR"
     gene            1969..>2181
                     /locus_tag="ENO07_06105"
     CDS             1969..>2181
                     /locus_tag="ENO07_06105"
                     /inference="COORDINATES: protein motif:HMM:PF00072.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDS01575.1"
                     /translation="MSDSNMDERSSKTIMLVDDDEIVVQSLGSFLELETEYNVIGYQS
                     PHEALERLKQKPVDVVISDFLMPDMNG"
BASE COUNT          611 a          478 c          634 g          458 t
ORIGIN      
        1 accgagcagc tggcctctct ggcctcgata gcagaggaag tggaatatat caagggggac
       61 gtgatctaca aggaacatga tccctcggat gcgctctacc ttgtgctgga gggcaaggtg
      121 aagctgcaca gaggcgacca ggaaatttcc actgcagaaa aagacgatgc ctttggtacc
      181 tgggctcttt ttgatgagga gccgagagtg gtaaccgcaa ccgcggccgc agactgccgt
      241 ctgcttcgtg tcgaccgcga ggatttcatc gatctgctgg ccgaccatgt gcagattgcg
      301 cagggcgtaa tcaagactgt ggtaaagagg ctcaggaatt tggtcgaacg agtagcctga
      361 catcggcgcc gggaccggat atgaaaatat ggttaaggca gataactccg atttaaaaag
      421 cctgcgggag tcggaagaga aataccgcaa gatgatcgag atggccagcg acgccatctt
      481 ttcgatcgaa ccggctgacg gcaccatact ggaggcgaat ctcagggccg agaagctgac
      541 cggcttcagc gttgacgagt tagtgggtaa gaaagtctgg gaactccatc ccgaatctga
      601 gtgggaggaa tccaggcggc tttttaacca ggtggtggaa agcggtaagg gctataccgg
      661 cgaactgcat tttctgcgaa aagataagac cctcctggat atagaaattt ccgcaggagt
      721 cataaattac ggggacaaga agatcattca gcgtatttgc cgtgacataa caagtcgcgt
      781 tcttttgcag agggagaata gacgcctgcg cgaatattat gaatatattc ttgatctaat
      841 gccggtcggt ctgggggtac gtaagaatat aaatggcgaa ccgaaagttg aatttgagaa
      901 taaaaagctg aaagagattt ttgaccgggc ccgggatgag ggctgcccat gcggttggga
      961 gcagtctatt gacgatcagc gggtggaaag cgagcagatc ctgagcgagg acggcgtcta
     1021 tgtggaggag aggatatttc ccaacaacag agtttaccag ttcaccaccg gctattttcg
     1081 ggatattaaa cataactggc gcgaactgaa tattgtgcag gacgttacga cccgataccg
     1141 gcttgagcag gagctgaaaa aagctaacga ggagctcgag cagaaggtgg aggaacggac
     1201 aagggagctg cgacagaaac agacgcagct ggtgcaggcg gaaaaaatgg ctgccctcgg
     1261 taatctggtg gcgggcgtag cccacgaaat caatacgcca ttgggagcac tcaacagcaa
     1321 caacgatctt ttcatccgct ctatcgagaa gctgaaggcc atggtctccg attctgctgc
     1381 gccgaccgaa tttcgtgaaa gcaagcagct cggtaagctg ttggaaaata tggaagatct
     1441 cacccggatc aataaaacag cctcttcccg aataatcaat attgtcagca gcctgcgcaa
     1501 attcgcccgc ctcgaccagg cggaaatgga tactgtaaat atccatgacg gcatagaaaa
     1561 tacgttgaca ctggtgcatc accagctcaa ggatcgggtg gaagttgaaa aggattatgg
     1621 ggatttgccg aagataagct gctatcccaa ccagcttaac caggtcttta tgaatatact
     1681 ggtcaatgcc agccaggcta tcgaggataa aggtaagatt attatcagaa cacgccgcca
     1741 gggcggcgat gctgttattg aattttccga taccggccgc ggaattcccg aggataaact
     1801 ggaacgggta tttgatccgg gatatacaac caaaggcgcc ggggtcggaa ccggcctggg
     1861 gctttcgatc gttcaccaga taactgagga ccataagggc cgaatcgagg tcgaatccag
     1921 agtgaatcag ggtacaacct tcagaatcat aattccaatt cgaggacgat gagcgatagt
     1981 aatatggatg agagatcgtc aaaaaccata atgctggtcg atgacgatga gattgtggtg
     2041 cagagcctgg gatcttttct ggaactggag accgaataca atgtgatcgg ctaccagtca
     2101 ccgcatgagg ctctggagcg gctgaagcag aagccggtgg atgtggttat atccgatttc
     2161 ctgatgccgg atatgaacgg t
//
LOCUS       DSEB01000340            2500 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1006184, whole genome shotgun sequence.
ACCESSION   DSEB01000340 DSEB01000000
VERSION     DSEB01000340.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1006184"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..425)
                     /locus_tag="ENO07_06110"
     CDS             complement(<1..425)
                     /locus_tag="ENO07_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015146930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylase"
                     /protein_id="HDS01576.1"
                     /translation="MKIILDTDTNLLIEENAGDIKKYDLYSRESFELISRYWVKVGWS
                     MKYPYTFSWLGRPVIQLPEDMIRVQEVLFKVQPDVIIETGVAHGGSLVFYASLCKALG
                     KGRVIGIDIEIRPHNRSAIESHFLFPYITLIEGSSTDVS"
     gene            complement(622..1542)
                     /locus_tag="ENO07_06115"
     CDS             complement(622..1542)
                     /locus_tag="ENO07_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017715484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDS01577.1"
                     /translation="MNKIIVTGATGFIGSHCLPLLVAEGYEVHAITTRKVRTEDRNII
                     WHCLDLFDSRMIETLFSEIRPTHLLHFAWTTEHGVYWNSMDNFNWLKAGVTILQKFAA
                     AGGERIVMAGTCAEYDWRYGLCSEQITPLNPHSVYGNCKKSLQQLTESFCTQTGLSWA
                     WGRIFFLYGPRENPLRLVPSVINALLQNREARCSAGTQIRDYLYVKDVASAFVKVMGS
                     GFNGSLNIASGKAVTVRDIVSAIAEKVGRPDLIKFGGVMENKDEPPMILADTRQLSVK
                     IGWQPEYDLKRGLAETISYWKGTAGAACDD"
     gene            complement(1539..2069)
                     /gene="rfbC"
                     /locus_tag="ENO07_06120"
     CDS             complement(1539..2069)
                     /gene="rfbC"
                     /locus_tag="ENO07_06120"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017715483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDS01578.1"
                     /translation="MIFTETTLKGAYIIDIEPIEDERGFFARSWCRKEFMDRGLNPNL
                     VQCNISYNKRKGTLRGMHYQAEPHQEAKLVRCTRGAIYDVIVDLRQGSSTCWQWIGVE
                     LSAGNRRMLYVPEGFAHGFQTLGDETEVFYQMSEFYFQEYTGGIRWDDPAIGIVWPEK
                     DPILSEKDMAFPPVII"
     gene            complement(2066..>2500)
                     /locus_tag="ENO07_06125"
     CDS             complement(2066..>2500)
                     /locus_tag="ENO07_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009768669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDS01579.1"
                     /translation="DRVGYLQEKEMNSGLDKISTYTDFAQQVHRTKRSLLRFLITAKN
                     EEKNVAGYGAAAKGNTLLNYCGIREDFLDYVVDRSPHKQGKYLPGSHIPVFSPQHISE
                     TRPDYLLILPWNLREEIMEQMRYIREWGGKFVVPIPEVQVFE"
BASE COUNT          646 a          686 c          498 g          670 t
ORIGIN      
        1 attgagacat cggttgaact cccctcaatc agggttatgt acggaaacaa gaaatgcgac
       61 tcaattgccg aacgattatg cggccggatt tcaatatcta tccctataac tctgccctta
      121 ccgagagctt tgcacaaact cgcataaaaa accagagacc ctccatgggc gacgccggtt
      181 tcaatgataa catccggctg gactttgaat agcacctcct gcacccggat catatcctca
      241 ggcaactgaa tcaccggcct ccccagccaa ctgaatgtgt acgggtactt catgctccaa
      301 ccgaccttaa cccagtatct cgaaatcaac tcgaacgatt cccggctgta gagatcatat
      361 ttctttatat cgccggcatt ctcttcgatc agtagatttg tatccgtatc aagtattatc
      421 ttcattagtc agctcgtaat caatgtaata tttagttttt cttgcttgat caaaaatgaa
      481 ttttcagcga ggtaattcgg attcatcaaa atcgcctgct gtatgcttct tctgccggct
      541 tctctgagcc cgacaatctg atatccactg ccaggaagaa acttgccctg cttattccga
      601 tttaagtcaa taatacaatc gttaatcgtc acaggccgcc cctgctgtac ctttccaata
      661 agagatggtt tcggcaagcc ctctcttcaa atcatattcc ggttgccaac caatttttac
      721 agacagttgc ctggtatctg cgagaatcat cggcggctca tctttatttt ccatcacccc
      781 cccaaatttg attagatccg gccgccctac cttctcagcg atagcactga caatatccct
      841 caccgtcaca gcctttcctg atgcgatatt caaagatcca ttaaacccgc tccccatgac
      901 tttgacaaaa gcgcttgcca catctttcac atagagataa tcacgtattt gagtaccagc
      961 actacagcga gcctctctgt tttgcagcaa cgcattgata accgagggaa ctaacctcaa
     1021 gggattttca cgcggaccat agagaaaaaa aattcttccc caggcccagc tcagcccggt
     1081 ttgtgtgcaa aacgattccg tcagctgctg caggcttttt ttacaattcc cgtacactga
     1141 atgaggatta agcggggtta tttgttcaga gcacaatcca tagcgccagt catattcagc
     1201 gcaggtgccg gccataacta tacgctcgcc acctgcggct gcgaacttct gcaggattgt
     1261 aacccccgcc ttgagccagt taaaattatc catggagttc caatagacgc catgttcagt
     1321 ggtccaggca aaatgaagga ggtgcgttgg cctgatctcg gaaaaaagtg tttctatcat
     1381 tctggaatca aaaagatcca gacagtgcca aattatattg cggtcttccg tgcgcacctt
     1441 gcgggttgta atcgcatgaa cttcataccc ctccgcaaca agaagcggca aacagtgaga
     1501 gccgataaac cccgtggcac cagtgacaat aatcttgttc atattattac cggcgggaaa
     1561 gccatatcct tctcggatag tatgggatct ttttccggcc agacaatccc aatcgccgga
     1621 tcatcccaac gaattccccc ggtatattcc tggaaataaa attctgacat ctggtaaaag
     1681 acctctgttt catctcccaa cgtctggaat ccgtgggcga atccctccgg gacatacagc
     1741 atgcgtctat ttcccgccga cagttcaacc ccgatccact gccaacaagt actggagccc
     1801 tggcgcagat cgacgatgac atcgtagatc gcccctctag tgcagcgcac cagctttgcc
     1861 tcctgatgag gctccgcctg gtaatgcatt ccacgcaatg ttcccttccg tttattatag
     1921 gaaatattgc attgaaccag gttggggttc agcccccgat ccatgaattc tttccggcac
     1981 cagctccggg caaagaaacc acgctcatct tctatgggtt caatatcgat aatatatgct
     2041 cccttgagcg tagtctcggt aaagatcact cgaacacctg cacttccggt atcggaacta
     2101 caaacttccc tccccattcc cgaatatacc gcatctgctc cattatctcc tcacgtaggt
     2161 tccagggcag gatgagcaga taatccggcc gggtttctga gatgtgttgc ggtgagaaaa
     2221 caggaatatg gcttcctggc agatactttc cctgtttatg aggactccgg tccacgacat
     2281 aatcaagaaa atcttctctg atcccgcaat agttgaggag ggtattacct tttgccgcag
     2341 ccccatatcc tgcaacattt ttttcttcat tttttgcagt aataaggaaa cgcagcaatg
     2401 acctcttggt cctgtgtacc tgttgagcaa aatcagtata tgtactgatt ttatcaagcc
     2461 cggaattcat ttctttttcc tgtagataac cgactctatc
//
LOCUS       DSEB01000341            6023 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000875, whole genome shotgun sequence.
ACCESSION   DSEB01000341 DSEB01000000
VERSION     DSEB01000341.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000875"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..2024
                     /gene="lon"
                     /locus_tag="ENO07_06130"
     CDS             <1..2024
                     /gene="lon"
                     /locus_tag="ENO07_06130"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDS01580.1"
                     /translation="PYLTAEVEELEEDSRSDVKLEALTRNVHEILKKVTELAPYLSEE
                     NYISAINQENPSKLADFVASNLNLNVEQKQEMLETSGVRERLEKLLAHLNKEYEVLEL
                     SRKIQAEAASEMGKLQKEYILKEQLKAIQKELGMTDERTEEINEFENKIRDARMPEVA
                     EEAAIKELDRLSKMNPSSAEYTVSRSYLDWMVSLPWRKESEDKLEIKPARKILDEDHY
                     DLEKVKDRILEYLAVRKLKTDLKGPILCFVGPPGVGKTSLGRSIARALGRKFERISLG
                     GMHDEAEIRGHRRTYIGSLPGRIIQGLKRAGTKNPVFMLDEIDKVGKDFRGDPASALL
                     EVLDPEQNDTFSDHYLDVPFDLSKVMFITTANMLDTIPDVLLDRMEVIRIPGYTDLEK
                     VQIAKKFLIPRELEEHGLKKSDLSFTSKALRNIINNYTRESGLRNLDRELATVCRKVA
                     KDVAMGKKGKVTVNPEDVQKYLGAPKFVRELAERVARVGVAPGLAWTPTGGDVLFIEA
                     TKMKGKGNLSLTGHLGDVMKESVQAAYSYIRTIAKKLDLDESAFENVDVHVHVPSGAV
                     PKDGPSAGIVMASAIASLFTGRPVKPRIAMTGEITLRGMLLPIGGLKEKSLGAYRAGI
                     KTIILPEANKKDIDEIPEELKKKLTYKFVKTIDEAIKLILEPPKKKSRK"
     gene            2577..3992
                     /locus_tag="ENO07_06135"
     CDS             2577..3992
                     /locus_tag="ENO07_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HDS01581.1"
                     /translation="MPDNVSFYQQVNDYFEKAAQYTKHPRGLLRQIKICNSVYHMMFP
                     VKRDNGEIEVIHSWRAEHSHHRLPVKGGIRYSPHANEDEVMALAALMTYKCAIVSVPF
                     GGAKGAIKIDRRQYSQAELERITRRYTYELIKKNFIGPGVDVPAPDYGTGQQEMAWIA
                     DTYRAISTDTLDAPACVTGKPITQGGVKGRVEATGRGVFFGIREACSDTDDMKRLGLS
                     FGLIGKSVVVQGLGNVGYHAAKFLQAGGAKIIGLAEYEGAIYDEKGLDVDAVVAHRKE
                     SGSILNFPGSKNIEKTHDALELECDILVPAALENQITEENASGIKAKIVAEAANGPVT
                     AKAHEILQKRNTMIIPDTYLNAGGVTVSYFEWLKNLSHVRFGRMGKRFEETTQRRILM
                     AIESATGKAFTDKEWQSLLHGADEEDLVNSGLEETMIGSYRKIREIKKQHNDEIDLRT
                     ASFINAIDLIAQAYMELGIFP"
     gene            4375..>6023
                     /locus_tag="ENO07_06140"
     CDS             4375..>6023
                     /locus_tag="ENO07_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015130764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HDS01582.1"
                     /translation="MIDDFLEGNTNAPDFPEGMDWLNSDRAFALKDFRGKMVLLDFWT
                     FCCINCMHVIPDLKKLEEKYDEELVVIGVHSAKFTNEKGTEAIRQAILRYGIEHPVVN
                     DRDFKIWRKYGVSAWPSFVLINPKGKIIGVHSGEGIYEPFDKIIGQAVEYFSARGELE
                     PRPLKLDLEKAEMLNTLLSFPGKVKADSASGRLIITDSNNDRIIITDPDGRIFEVIGS
                     GNNGQEDGSFEEAEFNRPQGTYMEDNILYIADTENHLIRRADLDNRTVETILGCGEQA
                     DRYNIPGIGTDVCLNSPWDLVKVGDILYIAMAGSHQLWTADLNTLQAKPFAGSAREAR
                     IDGPLAEAALAQPSGIATDGRKLYFTDSEISSIRSADIDPDGEIRTIVGEDLFEYGDI
                     DGDHTKARLQHPLGIVYHDGILYVADTYNSKIKKIDPEKRTSYTYAGTGEPGHQDGRL
                     NDAKFYESGGITYLNGKLYVADVNNHAIRVIDTKAGTVSTLQFSNLERLQREEEAEFT
                     GRIVELPKQNLKAGDSKVIFNLRLPEGYKYIENSPFSIDYKS"
BASE COUNT         1678 a         1409 c         1583 g         1353 t
ORIGIN      
        1 agccctatct gaccgccgag gttgaggaac tggaggaaga cagcaggtcc gatgtcaagc
       61 tggaagcgct gacgcgcaat gtgcacgaaa ttctgaaaaa ggtcaccgag cttgcgccgt
      121 atctgtcgga agaaaattat atctccgcca taaatcagga gaatccttcc aagctggcag
      181 actttgtggc ctccaatctg aatcttaatg tcgagcagaa acaggagatg ctggagacaa
      241 gcggtgttcg tgaacgtctc gagaaactcc tcgctcatct gaacaaagaa tacgaagtgc
      301 tggagctctc cagaaaaatc caggccgagg cggccagcga gatgggcaag ctccagaaag
      361 aatatatcct caaggaacag ctcaaggcga tccagaagga actggggatg accgatgagc
      421 gcacggagga gatcaatgaa ttcgagaata agatcagaga tgcacggatg ccggaagttg
      481 ctgaagaagc cgctataaaa gagcttgacc gcctttcgaa gatgaatcca tcgtcggcgg
      541 aatatactgt ttcgcgttca tacctcgatt ggatggtttc gcttccgtgg cgcaaagaaa
      601 gcgaggataa gctcgagatc aaacccgcca ggaagattct ggatgaggat cattacgatc
      661 tggagaaggt caaggatcga attctggaat atctggcggt acgcaagtta aagaccgatc
      721 ttaaaggacc tatcctctgt tttgtcggcc ctcccggagt tggcaagacc tccctgggac
      781 ggtcgattgc gcgcgccctg ggacgaaagt tcgagcggat ttccctgggg ggcatgcatg
      841 atgaggctga gatcagggga catagaagaa cctatatcgg atcactgccg ggcaggatca
      901 ttcagggttt gaaacgtgcc ggcacaaaaa atccggtctt catgctggat gagatcgata
      961 aggtcggcaa ggatttccgc ggtgatccgg ccagcgctct tctggaggtg ctcgacccgg
     1021 aacagaacga cacattttcg gatcattacc tcgatgtgcc ctttgatctt tccaaggtga
     1081 tgtttatcac caccgccaat atgctcgata caattccgga tgtgctcctg gatcgtatgg
     1141 aggtcatccg aattcccggg tataccgatc tcgaaaaggt gcagattgcg aagaaattcc
     1201 tgatacctcg tgagctcgaa gaacatggat tgaaaaaaag tgatctgagt ttcacctcga
     1261 aagcattgag aaatataatc aacaactaca cccgcgagtc cggcctcaga aatctcgacc
     1321 gtgagcttgc cactgtttgc cgtaaggtcg ccaaggatgt ggcaatgggc aagaagggca
     1381 aagttacggt caatccggag gatgtccaga aatatctggg cgcgcccaaa ttcgtgcgcg
     1441 agttggcgga acgtgtggcc cgtgtgggcg tagcgcccgg gctggcatgg accccgaccg
     1501 gcggtgatgt actctttatc gaggctacca agatgaaggg caagggcaac ctgtcattga
     1561 ccggtcatct gggtgatgtt atgaaggagt cggtgcaggc ggcttattcg tatatccgca
     1621 cgattgcaaa gaagctcgat ctggatgagt cggcatttga aaatgtcgat gttcatgttc
     1681 atgtgccctc gggtgcagtt cccaaggacg gtccctcggc tggaatcgtg atggcctcgg
     1741 ctattgcctc gctctttacc ggaaggccag tgaagccgag aattgccatg accggcgaga
     1801 taacgttacg cggtatgctc ctgccaatcg gcgggctgaa ggaaaagtcg ctgggcgctt
     1861 acagggcagg tatcaagact attatccttc cagaggcaaa caaaaaagat attgatgaga
     1921 tcccggaaga actcaaaaag aagctgacgt acaaatttgt caaaaccata gacgaagcca
     1981 taaagcttat cttagaacca ccgaagaaaa agagccggaa gtagaaatag atttagggta
     2041 ttgaatatat ccctgtgccg tcgggtcttt gatccggcca atgacgaaaa cgcattcccg
     2101 acggctcggg tgacccaatc gtcaggactt gttaataaac ctgaatgtac cggaatacgt
     2161 catcgcgagg agcgaagcga cgtggcgatc tccgcggtca ttctaagggc aaacgcggag
     2221 attcccacgg tcgtcctcga ccaaaggtcg ggactccctc ggaatgacgt aatacacact
     2281 ttcctgatat tgaggctttt tcagcaggcc ccaaagcttg gggcgcccga caactgatgg
     2341 atttcctgtt ttcaataatc ccacccctta cgggccgata aatcggcgcg acggagatgg
     2401 ggcacccaca tcagctttcc cggctccaag cttcaccact ccttcccatt ttctcccaaa
     2461 aaaatctaaa taccggcaac atttgttata atttgatgtt atattgaaaa tgttgaaaac
     2521 gtagattatt acggccataa atgcagccgt ttgcgggcat aaaaggagga gattcgatgc
     2581 ctgataatgt gagcttctat cagcaggtca acgactattt cgaaaaagcc gcacaataca
     2641 ccaaacatcc acgcgggctt ctgcgccaga tcaaaatctg caacagcgtc taccatatga
     2701 tgtttccggt caagcgggat aatggcgaga tcgaggtgat ccattcctgg cgcgccgaac
     2761 acagccacca ccgtcttccg gtcaagggcg gaatccgcta cagtcctcat gccaacgagg
     2821 acgaggttat ggcgctggcg gccttgatga catacaaatg cgcaatcgta agcgtgccct
     2881 tcggaggcgc caagggcgca atcaagatcg accgccgcca gtattcgcag gcggaactgg
     2941 aaagaatcac ccgtcgctat acgtatgagc ttattaaaaa gaattttatc gggccgggtg
     3001 ttgatgtgcc cgcacccgat tacggcaccg gccagcagga gatggcctgg atcgcggata
     3061 cgtatcgtgc gatctcaacc gatacccttg atgcgccggc atgtgtaacc ggcaagccga
     3121 tcacccaggg tggtgtcaag ggacgtgtcg aggcaactgg ccgtggtgtc ttcttcggta
     3181 ttcgtgaggc ctgttccgat accgatgata tgaaacgcct cgggctctca tttggactga
     3241 tcggaaaatc agtggttgtg caggggcttg gcaatgtcgg gtatcatgcc gccaaattcc
     3301 ttcaggcggg cggagcaaag atcatcggcc tggcggaata tgaaggcgca atttatgatg
     3361 agaagggtct ggatgttgat gctgtggtgg cgcatcgcaa ggaatccggc tcaatcctga
     3421 acttcccggg ctccaaaaac attgagaaga cccacgatgc gctcgaactc gagtgcgata
     3481 tccttgtacc cgccgcactg gaaaatcaga tcactgagga aaacgcctca gggatcaaag
     3541 ccaagatagt tgctgaggct gccaatggtc ctgttacggc caaggcccat gaaatactgc
     3601 aaaaaagaaa tacaatgatc attccggata catacctcaa tgccggcggt gttaccgttt
     3661 catattttga gtggctgaag aacctctcgc atgtccgttt cggacgcatg ggcaagcgtt
     3721 tcgaggaaac cacccagagg cgaattttga tggcgatcga aagtgctacc ggtaaagcat
     3781 ttacggacaa agaatggcag tcgcttttgc atggcgcaga tgaagaggat ctggtcaact
     3841 ccggactgga agagaccatg atcggctcat accgaaaaat cagggagata aaaaagcagc
     3901 ataatgatga gatcgatctc aggacggcct cattcataaa tgcaattgac ctgatagcac
     3961 aggcatatat ggaactggga atattcccgt aatacgacga ttatcgtcat cctgaacgca
     4021 ctgaaggata tggtttaatc ggaaagattc ttcggtcgtc ctcgcctgcg gcgggactct
     4081 ctcagaatga caattcaaat gttgcgtcat cctgaacaaa gtgaaggatc taattcgtta
     4141 gtaaaagcaa agactcccgg taaatagccg ggagtttttt tatttccacc atatgaacaa
     4201 atagaacaac taatcctgac aattgttgta tgatatacag gcaacaaaat aagagagaga
     4261 gattaagaaa tgagattttt cacgagtaca attatgcttc tggcattgat ttcagccgcc
     4321 ctggtcatcg cgtgtgatgc tcaggcgcag aatgatgtag aaagtgtgaa agttatgata
     4381 gatgattttc tggaaggaaa tactaacgct ccggattttc ccgaggggat ggactggctg
     4441 aattctgatc gcgcttttgc actgaaggat tttcgcggta agatggtact tcttgatttc
     4501 tggacattct gctgcatcaa ctgcatgcat gtcatccccg atttaaaaaa actcgaagaa
     4561 aaatacgacg aggaactggt cgtaatagga gttcattcag ccaaatttac aaatgaaaaa
     4621 ggcaccgagg caatccgtca ggcgatactt cgctatggta tagagcatcc ggttgtcaac
     4681 gacagggact tcaagatatg gcggaaatat ggagtcagcg catggccctc attcgtcctg
     4741 atcaatccca aaggcaaaat tatcggggtc cattccggtg aggggatata tgaaccgttt
     4801 gataaaatca tcggacaggc agttgaatac ttttccgccc gcggtgaact tgaaccgcgg
     4861 ccattgaagc ttgatcttga gaaagccgaa atgctgaaca cgcttctctc attccccggc
     4921 aaggtgaaag ctgattccgc atcgggcagg cttattatca cggattccaa taatgacaga
     4981 attattataa ctgatcctga cggcaggatt tttgaggtta tcggctcggg caataacggt
     5041 caggaagatg gctcattcga ggaagcggaa tttaatcgtc cccagggaac atatatggaa
     5101 gataacatcc tctatatcgc cgatacagaa aaccatttga tccgcagggc cgacttagac
     5161 aatagaactg tggagacaat ccttggatgc ggagagcagg ccgaccgtta caatattccc
     5221 ggtataggca ccgatgtttg tctcaattct ccctgggatc tggtcaaggt tggcgatatt
     5281 ttatatatcg ccatggcggg atcacatcaa ctatggacag ccgatctgaa tacactgcaa
     5341 gccaaaccat tcgccggctc ggcccgtgag gcacgtatag acggtcctct ggctgaggcc
     5401 gccctggctc agcccagcgg aattgcgacc gacggcagga aactttattt caccgacagc
     5461 gaaatcagct cgataagatc agcagatata gatccggatg gtgagatcag aactatcgta
     5521 ggcgaagatc ttttcgaata tggtgatatt gacggcgatc ataccaaagc tcgcctccag
     5581 catccattgg gaatagtcta ccatgatgga atcctgtatg tcgccgacac ttataattcc
     5641 aagatcaaga aaatcgatcc agagaagcgg acaagctaca catatgccgg taccggcgaa
     5701 ccgggccatc aagacggcag attgaatgat gcgaaatttt atgagtcggg cgggataaca
     5761 tatctcaatg ggaaattata tgtggccgat gtgaataatc atgctatcag ggtaatcgat
     5821 acaaaagccg gtacagtcag cacactgcaa ttttcgaatc tggaaagact gcagcgtgaa
     5881 gaagaggctg aattcaccgg acgtattgtg gaactgccca aacagaatct caaggcgggc
     5941 gacagcaaag taatattcaa cctcagactt cccgagggct acaaatacat cgaaaactct
     6001 ccattttcga tagattataa atc
//
LOCUS       DSEB01000342            2871 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004531, whole genome shotgun sequence.
ACCESSION   DSEB01000342 DSEB01000000
VERSION     DSEB01000342.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004531"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..946
                     /locus_tag="ENO07_06145"
     CDS             <1..946
                     /locus_tag="ENO07_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HDS01583.1"
                     /translation="HNLSMPEQEQICRKWIRQIARDIGPMRDVISPDIMVSPQHMIWM
                     MDEYETIQGERSAGITAGKPVRLAGSMGKEESTGYGLVYALREVLYDMGLSPAETTAS
                     VQGFGNVARHAIKLYHEIGGTTTCVSCWDQEDQTSYAYRKESGIDLNELSRVTDRFGG
                     INKEKARDLGYEVLAGESWIEQDVEILIPAALENQITEENVDKISKKVKIIAEGADGP
                     TTPAADKVIEERGIFVLPDLLANAGGAICSYFEQIQGNINYFWPQDEVLGKLDVKMTA
                     AFIKVSELAQKSKLYMRDAAYIIAVGRVAQACRDRGWV"
     gene            1020..>2871
                     /locus_tag="ENO07_06150"
     CDS             1020..>2871
                     /locus_tag="ENO07_06150"
                     /inference="COORDINATES: protein motif:HMM:PF01326.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDS01584.1"
                     /translation="MAVEHDPVEKLLLDLQERAKELNCLYRIEEVMNRPDATLDDVCE
                     GIIAAIPPGWQYPDICEAKLTLYEKVYTSRDFHETQWVQNAEIRVQDKTVGMLSVYYT
                     EEMPQADHGPFLKEETKLIRTIADRLGHFLMYRKVKGMFGEWRAAEEDLDGEGIKEWQ
                     VVLDLLHQTDRNLFLTVSLKMLNFLCWSGISEAEKLRRSSGADRKSDEDQIEKDSNRP
                     QQVRKFTISNELSKKIFSLASEHLTDDEISSKIEHWIQEDKLNFLIQVVNRNLSISEV
                     ADAIRRYHHIGDGVELHSPSRRGVLVSLIRRFLSVQLNYINIAKNYLEITDFYDLLEH
                     VIFSSESHGKLGGKAAGLTLASQIIRKSPEHADLLGDIKIPRTWYITSDMILHFMHYN
                     NFAEVVEQKYKDINQIRFEYPHIVQTFKNSHFPPEMVAGLSVALDDFEERPLIVRSSS
                     LLEDGVGMAFSGKYKSLFLANQGTKQQRLEALMDAIAEVYASVFSPDPIEYRTHNGLI
                     DFDEEMAIMIQEVVGNRVGDYFLPTFAGVGFSRNEFRWSPRIKRENGLLRLVPGLGTR
                     AVDRLSDDYPILIAPGQPDLRANTSIDEQLRYSPRQIDVINLKENSFYTMG"
BASE COUNT          748 a          677 c          811 g          635 t
ORIGIN      
        1 tcataacctc agcatgcctg aacaggagca gatctgccgc aagtggatca ggcagattgc
       61 ccgcgatata ggtcccatgc gggatgttat ctcgcccgac atcatggtca gcccgcagca
      121 tatgatctgg atgatggatg aatacgagac catccagggt gaaagaagcg cggggattac
      181 tgcaggaaag ccggtgcgtc tggcagggtc gatgggcaag gaagaatcga ccggatacgg
      241 cctggtttat gcgctgcggg aagttctcta tgatatgggc ctgagtcctg ccgagacgac
      301 agcatcggtt cagggtttcg gaaatgtcgc ccggcatgct attaagctct atcatgaaat
      361 cggcgggact acgacatgcg tatcctgctg ggatcaggag gatcagacat cctatgcata
      421 taggaaggaa tccggcatag acttgaatga gttgagccgt gtcactgaca ggttcggcgg
      481 aatcaataag gaaaaggcac gtgatcttgg ctacgaggtg cttgcgggtg aaagctggat
      541 cgaacaggat gtcgaaatcc tgataccggc ggctctggaa aatcagatta ccgaagaaaa
      601 tgtcgataaa ataagtaaga aggtgaagat tatagccgag ggcgctgacg gtcccaccac
      661 cccggcggcg gataaagtca tagaggaacg cgggattttt gtcctgcccg atcttctggc
      721 caacgccgga ggggctatct gcagttattt cgagcagatt cagggtaata tcaactactt
      781 ctggccccag gatgaggtct tgggcaagct ggatgtcaaa atgactgcgg cttttatcaa
      841 agtcagcgaa ctggcgcaaa aaagtaagct ttacatgcgc gatgcggcat acataattgc
      901 ggtaggacgg gtagcccagg cctgcaggga ccgtggatgg gtttagaacc cgggctggaa
      961 atttgcgccc gaatttcgta taataagatg gatacatcaa tgtctggagt gagaaaaaaa
     1021 tggccgttga acatgatccg gtagaaaaac tgcttcttga tttgcaggag cgcgccaaag
     1081 agcttaactg cctctaccgc atagaggaag ttatgaacag gccggatgct accctggatg
     1141 atgtttgcga gggcataatc gccgcaatcc ctcccgggtg gcagtatccc gatatatgcg
     1201 aggccaagct gacactgtat gagaaggtct ataccagtcg cgactttcac gaaacccaat
     1261 gggtgcagaa tgccgagatt cgtgtccagg acaagaccgt gggtatgctc agcgtttatt
     1321 atacggagga aatgccgcag gcggatcacg gccccttcct caaagaggag accaagctta
     1381 tcaggacgat agccgacagg ctcggtcatt tcctgatgta ccggaaggtc aaggggatgt
     1441 tcggtgaatg gcgtgccgca gaagaggacc ttgatgggga aggtattaag gaatggcagg
     1501 tggtgctcga tctgctccat caaactgaca gaaatctatt cctgacggtc tcattgaaaa
     1561 tgctgaattt cctgtgctgg agcgggatca gcgaggctga aaaactgcgg cgttcatcag
     1621 gagcggacag gaaaagcgat gaagatcaga ttgaaaaaga ttccaaccgg ccccaacagg
     1681 ttcggaaatt cacgatttcg aacgagcttt ccaaaaagat attcagcctg gcttcggagc
     1741 atttgaccga tgatgaaata tcgagcaaga tcgaacactg gattcaggag gataaactca
     1801 attttttgat ccaggttgtc aaccgcaacc tctcgatctc ggaggtggcc gacgccatcc
     1861 gccgatacca ccatatcggc gatggggtag aactgcattc acccagccgg agaggcgtgc
     1921 tggtttcgct tatacggaga tttttatctg tccagctaaa ctatataaat atcgcgaaaa
     1981 actacctgga aataaccgac ttctatgatc ttctcgagca tgttattttt tccagtgaaa
     2041 gtcatggcaa gctgggaggt aaggcggcgg gcctgacact ggcctcccaa ataatcagaa
     2101 agtcgcccga acatgctgac ctgttgggcg atataaaaat cccgcgcaca tggtatatca
     2161 cctcggatat gatcctgcat ttcatgcact acaacaattt tgccgaagtc gttgagcaga
     2221 aatacaagga tattaaccag attcgattcg aatacccgca catagtgcaa acattcaaga
     2281 attcacattt cccgccggag atggtggccg ggctatcggt ggccctggat gattttgagg
     2341 aacgtcccct gatcgtcagg agctccagcc tcctggagga tggggtgggg atggcctttt
     2401 ccggcaagta caagagcctg ttcctggcta atcagggcac caagcagcag cggctggaag
     2461 ctctgatgga tgccattgcg gaggtttacg cctcggtttt ctcccccgat ccgatcgaat
     2521 accgcaccca taacggcctg atcgattttg acgaggagat ggcgatcatg attcaggagg
     2581 tggtgggaaa ccgcgtgggg gattacttcc tgccgacctt tgccggggtg ggattcagcc
     2641 gcaatgaatt tcgctggtcc ccccggatca agcgtgaaaa cggactgctg cggctggtac
     2701 ccggactggg cactcgtgca gtcgatagat tatccgatga ttatccgata ctgatcgctc
     2761 ccggtcagcc cgacctgcgc gccaacacct cgattgatga acagcttcgt tattcgcccc
     2821 gccagatcga cgtgatcaat ctcaaagaga acagtttcta tacgatgggg a
//
LOCUS       DSEB01000343            6021 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000876, whole genome shotgun sequence.
ACCESSION   DSEB01000343 DSEB01000000
VERSION     DSEB01000343.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6021)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000876"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            736..1524
                     /locus_tag="ENO07_06155"
     CDS             736..1524
                     /locus_tag="ENO07_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01585.1"
                     /translation="MNKYFISTITLIVFLTSALFADDKSEAQDSTCHFFAPQEMSFFA
                     QTGDLDDQIYAFEDENAGNRKSVTTAVLLSLALPGLGELYVGRTGRAAGFFIAETGIW
                     ASFAYFKHKESWLEDDYINFAIQYAGVTPDGKDDFFYDMVGFYDNRDEYNKISRVYTR
                     TNPFFPETPDWDWEWESDELKTQFRDIKNDSRAQGRNANFTLGLALANRVISAVDAWW
                     SAKSYNRQFSPLMSKFHLKLTPSIADIVIGNGSPGFMLNFKHEF"
     gene            1751..2512
                     /locus_tag="ENO07_06160"
     CDS             1751..2512
                     /locus_tag="ENO07_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01586.1"
                     /translation="MYCDYSGNLFILREDRNSILKLSGEGAPIAHIGGFGFGVRQFNS
                     PVAMASSDGGLNLYVLDAENRRVVRLTNSLKWIDQFEIENEPNGKAIGELTGIAINSQ
                     KEIFISDPRNLRVIKFTQDGRFLEEIEGRSKFIEPGEIAIDDEDYLYVAGRDGKDVFL
                     FDNLGNYLKRYSPETNDSIGAIAVDRSYFCYLDSRSDKIKVFSKGLAYLFEIEPGRSG
                     DNRVVPVEIAPGQRGRIWVLDINNNRIYGYDPVEK"
     gene            2493..5837
                     /locus_tag="ENO07_06165"
     CDS             2493..5837
                     /locus_tag="ENO07_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01587.1"
                     /translation="MIRSRNRWTFRPHIFIAVLAACILSMSPSGKAAESRIDFPADTT
                     LTALTLPHANIFEGSIQLADSSGKLLEIQFERADYNRGLFYLAQPLRVTSRSTVRYRY
                     LGYDLPEYFFRRKLELERKEPEVIYREPEKDVYAESAPDFGRESFLLSGSKSIAVSAG
                     NAQDFDLDQTLNIQIQGEPVEGLTLTGSLSDRAQPQVAGLSSSLEDIESISVEARAKN
                     FRALLGDMDYNHEWGGISSFTKRLKGADAEFNLNAFDGRATVAGIKGRFRSVSFFARD
                     GISGPYSLKTPTGARTSIVSGTEKVYLDGRLLTPGASADYIIDYALGEITFNPKINLN
                     SRSRIVVDYEFLDQSYRRNYYAGELGFNAAAGSLRTSFGYLEIGDSKDNPVNLVLSDT
                     DREILEDAGDSRSQAVREGARFVGEGQGEYVLETDSLGNDYYRYAGDSLGNYDVSFSR
                     VDQGSGDYVYTGNGVYIYRGPNRGNYLPFEFIPLPSRTRAIYGAASGGAGDLLNFNIR
                     ASGSANDHNLFSALDDENNEGFMGEADVALIPVDTSKSNDIIRYAQTDLSITMQERSF
                     SLPGRSLQVELDRKWALNHDSIFKRSDQYEISQRLGLTDIFNLGGSLGRYRDGGYVSA
                     DKNLLSLDIQPTKGLIFEFKRDDRISDFKARDISSRLYQNSLSAGYSVKSLKITTGWA
                     DEKDTRVDTSGGIGFDRYYAHGSYAGFSAGISHKQQSRLDSVWRDDFEDFVIEAGMER
                     SFLNGKIRTESEIVRREVEYAEGGLPNLTETKSLSRINFKGPKNIFSGYLSYRFSRQL
                     VNRLARNFIKVEDGRGDYRLEDSIFVRDPFGDYIAVDELVESGGAGLSSEKSMNLRLD
                     FLRIIKPLKELSQLNSETHLNLEERGDNSFRLNLLYTLPFWETYPDNPIFSRFDLRQT
                     FNIGTSRGDIVSLGFEEIKTIDRIRDLQSDRYQRIIFEKVFVNVDQALNLRLEHRFKR
                     ERETSGYFGSADFGEHDILSELAFFSGRRFELSIRPRYLYDYSKTDDLTVNMYGAQIS
                     SAFGVAGQGRFAADFAYNRVESSEDGFIPYQFAAGNRPGDNFNWSTSFNFKYNKYITA
                     RLKYSADKIPGLQTRHRGTLSMRANF"
BASE COUNT         1521 a         1346 c         1594 g         1560 t
ORIGIN      
        1 ggaaaggata tccgatccgg atatgctgtt tgccgacaat tttaatgatt ttcccggttt
       61 tgaggatgat ctctataagc tcaagcatca ttctgtgggt cgggcttttc tgtattcatt
      121 gattcttccc ggcgccggtg aaatatacac aggttcaaag ctgaaggcaa gcctgttttt
      181 cgggatagag attttcagct ggtaccagta catcaacaaa tatggtgacg gacaggatct
      241 ggaagaccag taccgtgatt atgccaacca atactgggat cccggcgatt accgtgcctg
      301 gctggtggat gtcatgggtg taccgtctga tacactacca tacagaggcg aggatggcga
      361 accgcagaca tttacacatc atctgcccga tttcaaaacc cagcagtatt atgaaatgat
      421 cggcaaatat gaacaattcg tcttcggctg ggcggatact gattacaatg aaggcgattc
      481 gctctcgaaa acacatcggg tgacatatct tcaattgcgt gatgaatcca acagcaagtt
      541 tgatgcggcg cgcaattatg ctatcgtatc cattgccaac cgaattatct cggcttttga
      601 tgccgcactt tcagcccgca agctgaacaa gaaggcggat cgattttctg aagtccgttt
      661 gaaagccagg ctggccgact attacgggga gagaattccg aagattatct taacttataa
      721 cttctattaa tcagtatgaa taaatatttt atctcaacaa taacgctgat cgtgttttta
      781 acctccgcct tgtttgcgga tgataaatcg gaggcgcagg atagtacctg ccacttcttt
      841 gcaccccagg agatgagctt ttttgcccag acgggcgatc tggatgatca gatctatgcc
      901 tttgaggatg aaaacgccgg gaataggaaa tctgttacca ccgctgtttt gctttccctg
      961 gcgcttcccg gcctgggtga actctatgtg ggacgtaccg gccgtgcggc aggattcttc
     1021 attgccgaaa ccggtatctg ggcttcgttt gcctatttca agcacaagga atcctggctc
     1081 gaggatgatt atatcaactt tgcgattcag tatgcaggag taactccgga tggtaaagat
     1141 gattttttct atgatatggt cggattctat gacaatcgcg atgaatataa taaaatatcg
     1201 cgggtctata cccgcaccaa tccgtttttc ccggagaccc ccgattggga ttgggaatgg
     1261 gagtcggatg agctgaagac gcaattccgt gatatcaaaa acgactccag ggcacagggg
     1321 cgtaacgcca atttcaccct cggcctggcc ctggcaaacc gtgtaatctc cgctgttgat
     1381 gcctggtgga gtgcaaaaag ctataaccgg cagttttcac cattgatgtc gaagttccat
     1441 ctcaagctta cgccctcaat cgctgatata gtgattggaa acggctcccc gggattcatg
     1501 ctgaacttca agcatgagtt ttaaaattgc ttggcgcacg ccaaaaaata tgcataattg
     1561 gaacatgccc tgtttcaggt tcaatattaa taagatattg ctgaatttga tctttctttc
     1621 tcttttgctt cttttcgcat cgcaaaactc ctttgcggac aatccgccgg actctgctga
     1681 aaaatttttt gaaacctcgg cggtcataaa tattgacagt atcgaattat ctaagtcggg
     1741 gcggtatgat atgtactgcg attattccgg gaatcttttc atactcaggg aagaccggaa
     1801 ctcgatcctc aaactctctg gagaaggcgc cccgatcgcg catatcggag gatttggttt
     1861 cggagtccgt caattcaact cccccgtggc aatggcctca tctgacggcg gcctgaacct
     1921 gtatgtgctc gatgctgaga atagaagagt tgtcaggctg acgaattcgc tcaagtggat
     1981 agaccagttt gaaatcgaga atgagccgaa cgggaaggca atcggcgagc tgacaggaat
     2041 tgcaataaac tcccagaaag agattttcat atccgatcct cgtaatcttc gggttataaa
     2101 attcacccag gacggacgct ttctggaaga aatcgaggga cgttcgaaat tcatcgaacc
     2161 gggagagatc gcaatagacg atgaggatta cctgtatgtt gccggtcggg atggcaagga
     2221 cgtctttctt ttcgataatc tcgggaatta tctgaagcgc tattcacctg agacaaacga
     2281 ttctatcggg gcaattgcgg ttgaccggtc atatttttgt tatctggatt caagatcgga
     2341 caagatcaag gttttttcga agggattggc atacctgttc gagattgaac ccggcaggag
     2401 cggtgataac cgggtggtac cggtcgaaat cgcacccgga cagcggggtc ggatctgggt
     2461 tttggatatc aacaataacc ggatatatgg atatgatccg gtcgagaaat agatggacgt
     2521 ttcgtccgca catatttata gctgttctcg ccgcctgtat tctttcaatg tcgccctccg
     2581 gtaaggctgc tgagagcagg attgattttc cggccgatac cacattgaca gcactgactc
     2641 ttccgcatgc aaatattttt gaaggttcga ttcaattagc agattcatcc gggaaattgc
     2701 tggaaattca atttgagagg gccgattata accggggtct cttctatttg gcacagcccc
     2761 tcagggtgac atcgcgctcg actgttcggt atcgatatct gggttatgat ctgccggaat
     2821 attttttcag gcgcaagctc gagctggaaa ggaaggaacc agaggtaatc taccgtgagc
     2881 cggaaaagga tgtttatgcg gagtccgctc ccgacttcgg tcgagaatcc tttcttctga
     2941 gcggttccaa atcgattgcg gtatcggcag ggaatgcgca ggatttcgat ctggatcaga
     3001 ccctgaatat acaaattcag ggcgagccgg tagaggggct gaccctgaca ggttcgcttt
     3061 ccgaccgtgc ccagccgcag gtggccgggc tgtcgagttc gcttgaggat attgagtcta
     3121 tttcggtgga ggcccgggca aaaaacttcc gggccttact gggagatatg gattataatc
     3181 atgaatgggg cgggatatcc tcatttacaa aacgcctgaa aggggcggat gcagaattca
     3241 atctcaatgc ttttgatgga cgcgccaccg tggccggtat caagggcagg ttccgttccg
     3301 tgtccttctt cgctcgggat ggaatctcgg gaccttacag cctgaaaacc ccgaccggtg
     3361 cgagaacatc aatagtcagc ggcacagaga aggtttatct ggacggcagg ctcttgactc
     3421 ccggagcctc ggcggattac ataatcgatt atgcgctcgg ggaaataact ttcaacccta
     3481 agataaacct gaattctagg tcccggattg tggtggatta cgaatttctc gatcagtcgt
     3541 acagaagaaa ttactatgcc ggggagctgg gttttaatgc tgcggcgggc agcctcagaa
     3601 cttccttcgg gtacctggaa atcggtgatt caaaagacaa tccggtcaac ttggtattgt
     3661 ctgataccga ccgggagatc cttgaggacg ccggcgatag ccgcagtcag gctgtgcggg
     3721 aaggcgcgag atttgtgggc gagggccagg gcgagtatgt tctggagaca gattcgctgg
     3781 ggaatgatta ttatcgttat gcgggcgaca gcctgggcaa ttatgatgtc agcttctccc
     3841 gggttgatca gggaagcggt gattatgttt acaccggaaa cggtgtctat atttatcgag
     3901 gtccgaacag gggcaattac ctgccatttg agtttatccc gctcccctcc agaactcgag
     3961 caatctatgg cgcggcatcg ggcggagcgg gggatctgct caatttcaat atcagggctt
     4021 ccggatcagc aaatgatcat aatctattct ctgctctgga tgatgaaaat aacgagggat
     4081 ttatgggcga ggcagatgtg gcgcttatcc ctgtcgatac atcaaagtcg aatgatataa
     4141 tccgatatgc acagacggat ttgagcatca cgatgcagga gagaagtttc agccttccgg
     4201 gaaggtcgct tcaggtcgag ctggaccgca aatgggctct gaatcatgat agtatcttca
     4261 agcgttcgga tcagtatgaa atcagccaga gacttggatt gacagatatc ttcaatttgg
     4321 gcggttcgct tggaaggtat cgggatgggg gctatgtctc ggcggataaa aatctcttga
     4381 gcctggatat tcagccaacc aaaggattga tatttgaatt caagcgggat gatcgaattt
     4441 ccgatttcaa agcccgggat atttcaagcc gtctttatca gaacagcctg tcagcgggtt
     4501 attcagttaa aagcctgaag attacaacgg gatgggctga tgagaaagat acccgggtcg
     4561 acacatcagg cggaatcggt tttgaccgct actatgccca tggctcctat gcaggatttt
     4621 ctgccggaat atcccataag cagcagtctc gtcttgactc tgtatggcgg gatgattttg
     4681 aggattttgt tattgaggcg ggtatggaac ggtctttctt gaatggcaag atccgcaccg
     4741 aatcagagat tgtgaggcgg gaagtggaat atgccgaggg cgggctgccg aacctgaccg
     4801 agaccaaatc gctttccaga ataaatttca agggcccgaa aaatattttc agcggatacc
     4861 tttcataccg gttcagcagg cagctggtaa atcgtctggc cagaaatttc ataaaagtgg
     4921 aggatggtcg gggtgattat cgtctggagg attcgatttt tgtgcgcgat ccattcggag
     4981 attacattgc ggtggatgag ctggtcgaga gcggcggcgc cggactctcc tctgaaaaaa
     5041 gcatgaacct gcgcctcgat ttcctgcgga taataaagcc gctcaaagag ctgtcacagc
     5101 ttaattctga aacgcatttg aacctcgagg agcgcggcga taattctttt cgcttaaatc
     5161 tcctgtacac cctgccattc tgggagacat acccggacaa tccaatcttt tcacgcttcg
     5221 atttgaggca gactttcaat atcggcacca gtcgcggcga tatcgttagc ctcggattcg
     5281 aagaaatcaa aacaatcgac cggatccgtg acctccagag cgaccgttat cagcgcatca
     5341 tttttgagaa ggtctttgtc aatgtagatc aggccctgaa cctgaggctc gagcaccgct
     5401 ttaaacggga aagagaaaca tcgggatatt tcggctcggc cgatttcggc gagcatgata
     5461 ttctatcgga gctggccttt ttctcggggc ggcgattcga attatctatc cggccaaggt
     5521 acctgtatga ttacagcaaa acggatgatc tgaccgtaaa catgtacggt gcacagattt
     5581 cgtccgcatt cggagtggcc gggcagggaa ggtttgcggc tgattttgcc tataaccgtg
     5641 tggaatcgag cgaagatggg tttattccct accagttcgc ggcagggaat cgtcccggcg
     5701 ataacttcaa ttggagcacg agcttcaatt tcaaatataa caagtacatc accgcccggc
     5761 ttaaatacag cgccgataaa atccccgggt tgcaaacacg tcaccggggc acattgagca
     5821 tgagggccaa cttctgatga ggcatattat tcttacactt acggtgataa tggtttctgc
     5881 cgggtcctca tccggcgtta atattgtatt agagggggat aaaattttcg atcgaaagga
     5941 agcgtccgga atctgggatg aaatcaaaaa tgatagcaat aatgtggtga tgtttgagga
     6001 cagcctgtat atgagggccg a
//
LOCUS       DSEB01000344            5716 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000978, whole genome shotgun sequence.
ACCESSION   DSEB01000344 DSEB01000000
VERSION     DSEB01000344.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5716)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000978"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            89..2884
                     /locus_tag="ENO07_06170"
     CDS             89..2884
                     /locus_tag="ENO07_06170"
                     /inference="COORDINATES: protein motif:HMM:PF14522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01588.1"
                     /translation="MKASLNKFIVLAALIFAAAISGSMITAQEVDDCMMCHEDPDLTG
                     EVNGRETSVYVDRETYGNSIHGELECVDCHQDLYDAEFPHEEELDRVDCSMCHDDVAE
                     VYDASLHGQLVGQGVRLAPGCSSCHGSHDILPASNENSRTFKFNIPYMCGFCHKEGTE
                     VTQTYDIPEDSIVTHYSQSIHGVGLYQQGLMVTAVCTDCHTAHSVYNHANPKSSIHRN
                     NVANTCQQCHGQIEKVHTKVIRGELWKSEPDRVPVCIDCHPPHQIRQVFYEEGISNQD
                     CMECHGRPDLVGVQDGDTVSMYVDIDEHEHSIHRQVTCAQCHTGLSPEHEKRPCATVI
                     AKVDCSICHAEVVQTYGTSTHGKLFDRGDPDAPGCTDCHGEHSTLARRNPDSPTFPTN
                     VPRLCADCHRAGEVAAVRNQPEDRDVVAEYTMGIHGKGLLESGLVVTAMCTDCHTAHH
                     VLPKTDTASSVYKQNIPRTCAECHNGIYEKFSQSIHSPNISESEEILPVCDDCHQSHS
                     IIRADEDDFKLEIMSQCGQCHEDVTESYFDTFHGKVSKLGYTAAAKCYDCHGAHEILP
                     PDNPASTLSRQNIVETCGQCHPGSHRQFAGYLTHATHHDKTKYPVLFYTFWFMTTLLV
                     VTLTIAGTHTLMWLPKSFQMMKEHKAMRRAYRGRMEVRRFSTMHSVMHIFIIISFLGL
                     AITGMTLKFSYLGWAQFLSQVMGGFESTGYIHRICAIILIAVFGVHIYNVIKSKVQGR
                     KSWRHTIFGSESMMFNRRDAKEFFATIKWFIGAGPRPEYGRWTYWEKFDYFAVFWGVA
                     VIGSTGLMLWFPEFFTLFLPGWIINVATIIHSDEALLAVAFIFTVHFFNSHFRPDKFP
                     MDTVIFTGRVPVEELKNDRPREYKELIESRRIKHHMVEPLPPVVIRGLKLFGAIALIL
                     GLSLILLILYAEIFGYR"
     gene            3096..5693
                     /locus_tag="ENO07_06175"
     CDS             3096..5693
                     /locus_tag="ENO07_06175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HDS01589.1"
                     /translation="MNSKKSILRKRLLMPFILGWIFIISSMVDASHPNIFLFDKDFNE
                     INPITGENDDQPFSTKVTCGLCHDYDIITSGYHFQHGWDVISDTFGISRGKPWQLSPR
                     MMGGWSPPSFRQLAKKNNAHPDEIDLTAYDFIGNSSIGIQPSCGACHAGGGGMEFDRE
                     GNRYDEHLAENPELSETYDGDYYRSEWDKSGVVEADCFICHLVGYDFTSRVEQLEYRN
                     YEWAVVAGSRYGVIEGQVKYGDEPKVVYNTKYFNADGSISIDPSWPPPDDNCMFCHGA
                     SDSRKRGFSWNDIFNPDIHNQQGISCAACHPSGLDHQFAMGVADRAEPAGHIAHTMTE
                     CKECHEAGFLGALIPQHITVRPSHLRRITCEACHIPQLGRAAMQGYDVSTGDLETWTV
                     PDSVERIGERARWEPNFHRKQNKNIVPSNDMLGVFWGNIDSDGYIYILFMKEHEQAWD
                     LYSDQIEDDNDDGEPEVNTEAEIIAGLNAFKETLADNQRFSQVKPVYLKGNKVYGFDA
                     EGKLAILDEYEFETIFRYELHHNTAPARLALGYHGCADCHAPEAHFFKGELIKDPFDQ
                     YGKPVMALMGKELGCNRIAFDINAIHQKILSPIVSFLIIFVIFMVTLHYHRFGPKRIP
                     FVYGSGEVKRFSFLERAVHLFRLISFAFLALTGLIMAFNWYSWQNLLFASPKQMLDVH
                     IYMGIVFGVTTIMGIGLWSRDALFKAYDKVWMKMSGGYLGAKGEVPAGRFNAGQKMFY
                     WFTTIFGVLIIITGILLIIKYQFQLSTICLVSTIHNLIAFILIAGVLAHAYLGTIANP
                     GTWRVLVDGYVTKTWAKHHHPNWYQEVVKSKKQDIREEMSEAQTEGLPEEEIKKEESE
                     DGDDSEEEK"
BASE COUNT         1458 a         1374 c         1443 g         1441 t
ORIGIN      
        1 acattattct tgccattgct ctcgtatatc attaaaatag attctaatgt tttacggttt
       61 tgtaaagtat atgtaatcgg ggatatagtt gaaagcttcg ttgaataagt tcatcgttct
      121 tgcagccctc atctttgccg cggccatcag cggctccatg attacagccc aggaagtcga
      181 tgattgcatg atgtgccatg aagacccgga tctgaccgga gaggtcaatg gccgcgagac
      241 ctctgtctat gtggaccgtg aaacttacgg gaattcgatc catggcgaat tggaatgcgt
      301 ggattgccac caggatcttt atgatgccga atttcctcac gaggaagaac ttgacagagt
      361 ggactgcagc atgtgtcatg acgatgttgc ggaggtgtat gacgccagcc tgcatggcca
      421 gcttgtcggc cagggagtaa ggctggctcc cggatgttca agttgtcacg gttcccatga
      481 cattttgccc gccagcaatg aaaactccag gactttcaaa ttcaatattc cctatatgtg
      541 tggcttctgt cacaaggaag gcacagaggt tacacaaact tacgatattc ccgaagatag
      601 tattgtgacg cattattcgc agtctattca cggggtcggt ttgtaccagc agggattgat
      661 ggttacagca gtctgcaccg attgtcatac tgctcattca gtttataacc acgccaatcc
      721 aaaatcctcg attcaccgga ataatgtggc caacacctgc cagcagtgtc acggtcagat
      781 cgagaaagtg cataccaaag tcatccgtgg agaattatgg aaaagcgagc ccgaccgggt
      841 ccctgtctgt atagattgtc atcctcccca ccagattcgt caggtgtttt atgaggaggg
      901 catatcgaac caggactgca tggagtgcca tggccggccc gacctggttg gagtgcaaga
      961 cggggatacc gtttcaatgt atgtggatat cgatgaacat gaacattcca tccacaggca
     1021 ggtaacctgc gcgcaatgtc atacgggact gagcccggaa catgagaaga gaccctgcgc
     1081 gaccgtaatt gcgaaggttg actgctccat atgtcatgcc gaggtggtcc agacgtatgg
     1141 caccagcacc cacggcaagc tctttgaccg gggcgacccg gacgctcccg gctgtacgga
     1201 ctgccatggc gagcatagta cactggcccg cagaaatccg gattcgccaa cctttccgac
     1261 caacgtcccg cggctctgtg ctgattgcca tcgggccggg gaagtggccg ccgtccgcaa
     1321 tcagcccgag gatcgggatg ttgtggcgga atataccatg gggatccatg gcaaggggct
     1381 tctggaaagc ggacttgttg taaccgccat gtgtaccgat tgccatacag cacatcatgt
     1441 tctccctaaa accgacacag cgtccagcgt ttataaacaa aatatacccc ggacctgcgc
     1501 tgaatgtcat aatggcatct atgaaaaatt ctctcagagt atccactcgc ccaatatttc
     1561 agagagcgag gaaatattgc cggtctgcga tgactgccac caatcgcatt caatcatacg
     1621 cgccgacgaa gacgatttca agctggaaat tatgtcgcag tgcgggcaat gtcatgagga
     1681 cgtgacggaa tcctatttcg atactttcca cgggaaggtc tccaagctcg gttatactgc
     1741 tgctgctaaa tgttatgact gtcatggcgc tcatgaaatt ctgcctcccg ataatcccgc
     1801 ttcgaccctg tcacggcaga atattgtgga aacctgcggc caatgtcatc ccggttcgca
     1861 caggcagttt gccggctacc tgacccatgc cacgcatcac gataaaacca aatacccggt
     1921 gcttttctat actttctggt ttatgaccac cctcttggtg gttactctta cgatagcggg
     1981 cacgcataca ttaatgtggc ttccgaagtc atttcagatg atgaaagagc acaaagcgat
     2041 gcgccgggca taccgggggc gcatggaggt acggcgcttc agcaccatgc acagcgttat
     2101 gcacattttc ataataatca gctttctcgg ccttgccata accggcatga ctttgaaatt
     2161 ctcctatctt gggtgggcgc agtttttgtc gcaggtcatg ggaggattcg aatcgaccgg
     2221 gtacatacat cgtatctgcg ccataattct catcgccgta ttcggggtgc acatatataa
     2281 tgttattaag tccaaggttc aaggcaggaa gtcctggcgc catacgatct tcggatcgga
     2341 atcgatgatg ttcaacaggc gcgatgctaa ggagtttttt gccaccataa aatggtttat
     2401 cggcgccggg ccgcgtccgg aatacgggcg ctggacatac tgggagaaat ttgattattt
     2461 tgcggtcttc tggggcgtcg ccgttatcgg ctcgaccggt ttgatgctct ggtttccaga
     2521 gttctttaca cttttcctgc caggatggat aataaacgtg gccaccataa tccactcgga
     2581 tgaggcgctg ctggccgtgg cctttatctt taccgtgcat ttcttcaatt cccacttccg
     2641 cccggataag ttccccatgg atacggtgat atttaccgga cgtgttcctg tcgaagaact
     2701 gaaaaacgat cgtccccggg aatacaagga attgatagaa tcacgccgca taaaacacca
     2761 tatggtggaa ccgcttcctc cggtggttat caggggactg aaactttttg gagcgatcgc
     2821 tctcatactt gggctctcgc taatcttgct gattctttat gccgaaattt ttgggtatag
     2881 ataaatccga atctgttaaa attaagtcac atagtcgaat attctgctaa ggatggcagg
     2941 catttttatc tttttatata agtcatttgc attattgtcg tatataattt aacttgatat
     3001 gccgtgagta attcatctgg cccatacact tgcgaaatgt atctcacggc agcattaaag
     3061 atggcagtaa aatttcaaaa tagggattgc gccaaatgaa ctcaaaaaaa tcgatattgc
     3121 gaaaacgact gctgatgccc ttcattctcg gatggatatt catcatatcc tctatggtgg
     3181 acgccagcca tcccaatatt tttctttttg ataaggattt taatgaaata aacccgatca
     3241 ccggcgagaa tgacgaccag cccttcagca ccaaagttac ctgcggactg tgccatgatt
     3301 atgatattat aacttcggga tatcattttc agcatggctg ggatgtgatc tcggatacgt
     3361 tcggaatatc ccggggaaag ccctggcagc tctccccccg catgatggga ggatggtcgc
     3421 cgccgtcatt tcggcagctg gccaaaaaga acaatgctca tcccgatgaa attgacctta
     3481 ccgcatatga tttcataggc aactcttcga ttggtattca gccctcctgc ggcgcctgtc
     3541 atgccggagg gggcggcatg gaatttgacc gtgagggtaa ccgctacgat gaacacctgg
     3601 cagaaaatcc cgaactcagt gaaacatatg acggcgacta ttaccggagc gaatgggaca
     3661 aaagcggcgt ggtcgaagcg gactgcttta tttgtcacct ggtgggctac gattttacct
     3721 ccagagtgga acagctcgag taccgcaact atgaatgggc cgtagtagca ggctcgagat
     3781 atggagttat tgagggccag gtgaaatacg gcgatgagcc caaggtcgta tataatacaa
     3841 aatattttaa tgctgacggc agtatcagca ttgacccatc ctggccgccg cctgatgaca
     3901 actgcatgtt ttgccacggt gcctcagatt caaggaagcg aggattctcc tggaatgata
     3961 tcttcaatcc cgatattcat aatcagcagg gaataagctg tgccgcctgc catccctcgg
     4021 ggctggatca ccagtttgcc atgggcgtcg ccgacagagc cgaacctgca gggcatatcg
     4081 cccatacaat gaccgaatgc aaggaatgcc atgaagccgg ttttctgggt gccctgatcc
     4141 cgcagcatat caccgtgcgg ccgtcccatc tgcgacgaat tacatgtgaa gcttgccaca
     4201 ttccccagct cggaagagcg gccatgcagg gatatgatgt ttcaaccggg gacctggaaa
     4261 catggacagt tcccgactct gtcgagagaa tcggtgaaag ggccaggtgg gagcctaatt
     4321 ttcatcgcaa acagaataag aacatcgtgc cctccaacga tatgctggga gttttctggg
     4381 gcaacatcga ctctgatggc tatatatata tcctgttcat gaaggaacat gagcaggcat
     4441 gggaccttta ttccgatcag atagaggacg acaacgatga cggcgaacca gaagtcaata
     4501 ctgaagctga gatcatcgcg ggtctgaacg ccttcaaaga gactctggcg gataatcagc
     4561 gtttcagcca ggtcaaacca gtatatctca aaggcaataa agtttatggt tttgacgctg
     4621 agggtaagct ggctatcctg gatgaatacg aattcgaaac gatattcaga tatgagctgc
     4681 atcacaatac cgcaccggca cgcctggcat tgggttacca tggctgtgca gactgtcatg
     4741 ccccggaagc gcacttcttt aaaggtgagc ttataaaaga tccttttgac caatatggaa
     4801 agccagttat ggcattgatg ggaaaagagc tgggatgtaa tcggatagca tttgatataa
     4861 atgccatcca tcaaaagatt ctcagcccta tagtcagctt tctgattatt ttcgtgattt
     4921 tcatggtgac cctgcattat catcgcttcg gccccaagcg catcccattc gtctacggtt
     4981 ccggtgaggt gaagcggttt tcttttttgg aacgggcggt acatcttttc cgcctgatat
     5041 ccttcgcatt cctggccctg acaggcctga taatggcttt caactggtac agctggcaga
     5101 atctgctctt cgcatccccc aagcagatgc tggatgtgca tatatatatg ggaattgtat
     5161 ttggagttac cactattatg ggaatcgggc tgtggtccag ggacgccctt ttcaaagcgt
     5221 atgataaagt ctggatgaag atgagcggcg gatacctggg cgccaagggc gaggttcctg
     5281 ccggacgatt taatgcaggc cagaagatgt tctactggtt caccacgatt ttcggggtgc
     5341 tgataataat cacggggatt ctgctaataa ttaagtacca gttccagctt tccaccatct
     5401 gcttggtttc gaccattcac aatttgattg cctttatcct tatcgccggt gtacttgccc
     5461 acgcgtactt gggtacaatt gctaatccgg gaacctggcg tgtgttggtc gacggatacg
     5521 ttaccaagac ctgggccaaa catcatcatc ccaactggta ccaggaagta gtgaagtcaa
     5581 agaagcaaga cataagagaa gagatgtctg aagcccagac agagggattg ccggaggagg
     5641 agataaaaaa agaagagtcc gaagacgggg acgattccga agaagagaag taactcagtc
     5701 ttataagata taattg
//
LOCUS       DSEB01000345            1682 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1014791, whole genome shotgun sequence.
ACCESSION   DSEB01000345 DSEB01000000
VERSION     DSEB01000345.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1014791"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..524
                     /locus_tag="ENO07_06180"
     CDS             <1..524
                     /locus_tag="ENO07_06180"
                     /inference="COORDINATES: protein motif:HMM:PF08278.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01590.1"
                     /translation="LNSLKNELARLMQKGRGPAREEVKQTAPAALQRIQLQILAVLIE
                     NMNLLEKALLELSEDDFSDPVIKGIYNKMLLLRSKNPSMDVSYLLNHISDPEQSKIIS
                     QASNMELGTRDTERLFSDFARRLKALNLEGRRKTLISRIASAEKAGDLRRAEELTAEL
                     KELTEKSSAEEEA"
     gene            complement(528..1193)
                     /locus_tag="ENO07_06185"
     CDS             complement(528..1193)
                     /locus_tag="ENO07_06185"
                     /inference="COORDINATES: protein motif:HMM:PF02674.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDS01591.1"
                     /translation="MCVNWLDFVLVAGLLIALILGSKIGLVRSVMRFFALAAGIIITT
                     NNADIIAIEVARHIDASPMIIAIISFVILLAILYGIFRLVVIMFYKVSQIQSLGRTDK
                     IGGGVMGVVRGWVFFGFLFFLLALTPVPDGFHRMVDNSTLSEPMMRTITFLYDGTKML
                     HPESRSFVDQVQNSLEETNKTRFDSIGEPENSFERQRANDAVREKMQVIQRYFGPLHR
                     EET"
     gene            complement(1223..1669)
                     /locus_tag="ENO07_06190"
     CDS             complement(1223..1669)
                     /locus_tag="ENO07_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDS01592.1"
                     /translation="MTISQRIESDIKEAMKAREAQKLSYLRNFKSALKYKEIDKKDKL
                     TEEEEIQVLSSLVKQLRDSLEQAEKAGREDHASRAKDELDLAMTYMPEQMSEEEIEKL
                     AHEVIEEVDAKGPSDMGIVMKGLMPKVKGKADGSVVKAVVMRLLKG"
BASE COUNT          446 a          448 c          382 g          406 t
ORIGIN      
        1 atctgaacag tttaaaaaat gaactggccc gcttaatgca gaaaggacgc gggcccgcgc
       61 gggaggaagt caaacaaacg gcccccgcgg cattgcaaag gatacagctt caaattctgg
      121 cagtattgat cgaaaatatg aatttactgg agaaggcgct gctggagtta tccgaggatg
      181 atttcagtga ccccgtgata aaaggaattt acaacaagat gctgctgctg cgcagtaaaa
      241 atccctccat ggatgtttca tatcttctga atcatatcag cgacccggag cagtccaaaa
      301 taatctcgca ggcctcaaat atggaactgg gaaccaggga tacagaacgg ctcttcagcg
      361 attttgcccg aaggctgaaa gcgctgaacc ttgaaggcag gcgcaagaca ttaatatccc
      421 ggatcgccag cgccgagaaa gcgggtgatc ttagacgggc cgaagagctc accgccgaat
      481 tgaaagaact aaccgaaaaa tcttcagcag aggaagaggc ttaagggcta agtctcttcc
      541 ctgtggagcg ggccgaaata cctttgaatg acctgcatct tctctctcac agcatcattc
      601 gctctctgcc tctcaaaaga attctccggt tcaccgatcg aatcgaagcg ggtcttattg
      661 gtctcctcca gggaattctg cacctggtca acaaacgatc tgctttccgg atgcagcatt
      721 ttggtgccgt catacaggaa tgtgatcgtc ctcatcatcg gttcggagag cgtagagttg
      781 tccaccatac ggtgaaatcc atcgggcaca ggggtcagtg ccaggagaaa gaacagaaaa
      841 ccaaaaaaca cccatcctct tacaacgccc ataactccgc ccccgatctt atcggtccgc
      901 cccagcgatt gaatctggga aaccttgtag aacataatta caaccaggcg gaatatcccg
      961 tacagaattg ccagcaggat tacgaaactg attatggcaa taatcatggg cgaggcgtcg
     1021 atatgacggg cgacttcgat tgcaataatg tcggcattat tggtggtgat aataatgcct
     1081 gcggccaatg caaaaaatct catgaccgag cgcacaagac cgatcttgct gcccagtatg
     1141 agagctatca aaaggcccgc aaccagcacg aaatcaagcc aattcacaca cagcctccat
     1201 gcttataaat tgactgctgt tattatccct tcaaaagccg cattaccacg gctttgacaa
     1261 cgctgccatc cgctttcccc ttgaccttgg gcatcaatcc cttcataaca atgcccatat
     1321 ccgaggggcc cttggcatcg acttcctcga tgacttcatg agcgagtttc tcgatctcct
     1381 cctcgctcat ctgctccggc atataagtca tcgccaggtc gagctcgtcc ttcgccctgg
     1441 atgcatgatc ttcacgcccg gccttctcag cctgctcaag cgaatcccgc aactgtttaa
     1501 ctaatgaaga gaggacctgt atctcctcct cttcagtcag tttgtccttc ttatcgatct
     1561 ctttatattt cagggccgat ttgaaattgc gcaggtaact gagtttttgg gcttcccgcg
     1621 ctttcattgc ctcttttatg tcagattcga ttcgttggct gattgtcatc agatcaatta
     1681 tc
//
LOCUS       DSEB01000346            2919 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004360, whole genome shotgun sequence.
ACCESSION   DSEB01000346 DSEB01000000
VERSION     DSEB01000346.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004360"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            941..1738
                     /locus_tag="ENO07_06195"
     CDS             941..1738
                     /locus_tag="ENO07_06195"
                     /inference="COORDINATES: protein motif:HMM:PF02683.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HDS01593.1"
                     /translation="MQLFYALATLGGGSGGFGGGGGFDIGPLAPVADVGLWAAFIAGI
                     VSFISPCVLPLIPGYLSFISGVSIDKLHSREDRAEVVKKTALTSFIFVLGFSTVFILL
                     GASATAVGNILKEYQDIISKVFSVIIFLLALHFLGVYRLKFLAFEKKVHATVKPLNLI
                     SIYLVGMAFAFGWSPCVGPLLASVLAMAATREQVGQGVMLLSSYSLGLGIPFMATAIA
                     LNSLLGVFGWVKRHFRLIEIISGILLIIVAVLMFFGQLEVLANRLVQ"
     gene            1826..2575
                     /locus_tag="ENO07_06200"
     CDS             1826..2575
                     /locus_tag="ENO07_06200"
                     /inference="COORDINATES: protein motif:HMM:PF09837.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2064 domain-containing protein"
                     /protein_id="HDS01594.1"
                     /translation="MPAKKKQQALIIFLEDSNRDIIHSKLTDYLSQDEKNKLYSAFLE
                     DTIYTCLNLKDVLLKINYPSEAARCIINKSIQGLEKRLPPKLIKTLKASGVEAEMIKG
                     KSQGERLKHAFEQTFTNGHSPVLLIGCVTPTLSKKVLQDAYRKLKRSDVVFGPTLEGS
                     YYLVGLNRLENDVFENVEWAGDKPVYSQMVGAVSGGTLNWQELELWYDLRQPEDFEFL
                     IRDINFFRQIGDENSASKTEEVLNEILKKMP"
     gene            2575..>2919
                     /locus_tag="ENO07_06205"
     CDS             2575..>2919
                     /locus_tag="ENO07_06205"
                     /inference="COORDINATES: protein motif:HMM:PF02633.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HDS01595.1"
                     /translation="MAHKLERMLWQQIRDLVPDMIDTVVLPIGTIEAHGAACLGTDVL
                     IPEDISEFVCDKFNFIKAPPIWYGITKSLLDYPGSLTITPENLMNYVYDLLKSFQRHK
                     FRKIIIMNGHGGN"
BASE COUNT          767 a          651 c          743 g          758 t
ORIGIN      
        1 gattcatcaa tgctcggcac caaagcctta ttaatgcttt cgccgtcgga aattgtaaac
       61 cagcgtcagt tggccgatgc ccagcctgat ggtactctca acaagccttt taatccgccc
      121 gatcttctga cgaaggcagg cgaaattctg ggggaagatt tcgggatgaa gcagaataag
      181 cgtgatgacc cgcagcagat ttcagtttcc gatatgggca tgaacggtga ggattcttca
      241 gaagaaatcg attttgactc ggtttttgag aatgacgagg aggatcctgg gaccggggat
      301 gttcacttcg ccgagatact tgatgaggat aatgcctccg gcggcaaacc aaagcgaccc
      361 cgcgattcgg ctgaagataa ggatgatgag ggaatcaagc tggcagatga tcaattcggg
      421 ctggaagaac cgtttgatcc tcctgaagtt gaaactcccc atgattataa ctggtttatt
      481 cgcgagatga aaaatgaaat cgaggataaa ggcaaggata agaagaaggc ttcccagccg
      541 gctcagcccc gaaagtccga aaagaaggcc ggttccaaat cggcagagga tttaacaggg
      601 catttcatgg ttgaggaaat tgactccgcc aaagtgtatt ccgaggagcc cgaggagcag
      661 gagaagggct ttgctgatac ccctgatgaa tttgatcaat tctcctccgg gaagaagtcc
      721 ggtttggtag aagacgatga agagaacaag ctcctcatgg tggagagatt gctggtaaag
      781 gaactgtcca ggcaacttgc ccagaaaatt attgaccaga taccccgcaa aaaactacat
      841 caaattatcg acgaattatt tacggaacta aaaaagtatt gacaacgtta attttccgat
      901 gcacaattag gctttaataa ctgttccgca gaggagaatt atgcaactat tttacgcgct
      961 tgccacactt ggcggaggtt ccggaggatt tggcggaggc ggcggattcg atataggacc
     1021 gctggcgccc gttgcggatg tgggattatg ggccgctttt attgcgggaa ttgtgtcctt
     1081 tatctccccc tgcgttctgc ccctgatacc cggatatctt tcatttatat cgggggtctc
     1141 gatcgacaaa cttcattccc gtgaagaccg cgcagaggta gtaaagaaga ccgcgctgac
     1201 ttcctttatc tttgtgctcg gtttctcgac tgtatttatt cttctggggg catcggcaac
     1261 ggcggtcggc aacatcctga aagaatatca ggacatcata tcgaaagtgt tctcagttat
     1321 tatattcctg ctggcgctcc attttctggg agtataccgt ctaaaattcc tggcattcga
     1381 aaagaaagtg catgccacgg tcaaaccgct caacctcata agtatttatc tggtggggat
     1441 ggccttcgcc tttggatggt ccccctgcgt cggaccgctt ctggcatcgg tcctggctat
     1501 ggcggccacg cgggaacagg tggggcaggg agttatgttg ttgtcttcct attcacttgg
     1561 actgggaata ccatttatgg ccacggcaat cgccttgaat tctcttctgg gtgttttcgg
     1621 gtgggtcaaa agacatttcc gcctgataga aattatcagt ggaatacttc taattatagt
     1681 tgcggttctg atgttcttcg ggcagctgga ggttctggcc aacagattag tgcagtgatt
     1741 ttcggatgat accttatttt cgcttgaaat ttccggtgct atttttatat tgagtgccaa
     1801 aggttcaccc ttagaaaagg aaattatgcc cgcaaagaaa aagcaacagg cgcttatcat
     1861 ttttctggaa gatagtaacc gggatattat acacagcaag ctcactgatt atctttcaca
     1921 ggatgagaag aacaagctct attctgcatt cctcgaggat actatttaca cctgtttgaa
     1981 tcttaaggat gttttgctga agattaatta tccttccgaa gctgcacgtt gtattataaa
     2041 taaatcgata cagggtctgg aaaagagatt gcctcccaaa ctcataaaga ctctcaaagc
     2101 cagcggggtc gaggctgaaa tgatcaaggg caaaagccag ggcgaacggc tcaagcatgc
     2161 ttttgagcag actttcacaa acggccattc cccggttctt ctaatcggtt gtgttactcc
     2221 cactctatcg aaaaaggttc tgcaggatgc ataccggaag ttaaaaaggt ccgatgtcgt
     2281 cttcggtccc actctggagg gaagttatta tctggtcggc ctgaaccgtc tcgaaaacga
     2341 tgtcttcgaa aatgtggagt gggcagggga taagccggtt tacagccaga tggtcggcgc
     2401 tgtatcgggt ggtaccctga actggcagga gctcgagctg tggtatgatc tcaggcagcc
     2461 cgaggatttt gaattcttga ttcgcgatat aaatttcttc cgccagatcg gcgatgaaaa
     2521 ttccgcctcc aaaacggaag aagtactcaa tgaaattttg aagaagatgc cctgatggca
     2581 cataagcttg aaagaatgct ctggcagcag atcagggacc tggtccccga tatgatcgac
     2641 acggtcgtgc tgccgatcgg tacgatcgag gcccatgggg cggcatgcct cggcaccgac
     2701 gttctcattc ccgaggacat ctccgaattt gtttgtgata aattcaattt tatcaaggcc
     2761 cctcctatct ggtatggaat cactaaatcc cttctggact accccggctc gctcaccatc
     2821 acacctgaga atctgatgaa ctatgtttat gacctgctca aatcattcca gagacataaa
     2881 ttcaggaaga ttataatcat gaacgggcat ggggggaac
//
LOCUS       DSEB01000347            1929 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1010955, whole genome shotgun sequence.
ACCESSION   DSEB01000347 DSEB01000000
VERSION     DSEB01000347.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1010955"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            514..>1929
                     /locus_tag="ENO07_06210"
     CDS             514..>1929
                     /locus_tag="ENO07_06210"
                     /inference="COORDINATES: protein motif:HMM:PF00144.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C beta-lactamase-related serine hydrolase"
                     /protein_id="HDS01596.1"
                     /translation="MRHRLWQRLTVLVRFLLILFFTTGCILFPTGICFSGPNADYSYR
                     IPPVIDDGWKTESLYKTTIDADKLIDLIRHIRNGDYKNIHGVLLVKDGKLMLEEYFHG
                     FDREKPHQIRSATKSIGSILTGIAIDHRFIKDIKEKIYPYFKEYDIGQEWDARVRDVT
                     LESLLTMTSGYACNDHATPKFECEKAMYGADDWVEYALNLPMAHKPGEHWAYNSSSLM
                     LVSQIISKTSTMTIPDFAERYLFEPLGINEFHWGFSPKGRAWIAGNAKMKPRDMAKIG
                     LLMLEGGKWKGKQIVSEKWIGESTREHEKSENNWGYGYLWWVGEQLIGEQIITGYWAA
                     GNGGNYIFVCPGLDLVAVFTGGNYNSILELQTLGMLINYIIPAMLPPILPREIIKLDP
                     AILDSCVGDYQLRQGDIRVSVFRKSDTLYCTILERTMRMYPETDDLFFIPDDILGNWT
                     FKIERNEKGDVTSATGYTAFQA"
BASE COUNT          523 a          424 c          503 g          479 t
ORIGIN      
        1 atcgtggacg gactcagatc gtttggcata cagaagatcg ccgtcaactg cacctattac
       61 gacaccgctt ggcgcgatgg ttttgcagcc tttttgagac tatgcggttt cgatacgata
      121 catgtctcaa cgctctcaga ccaggggctc accgaatctg atgcgaaaat ggaagactac
      181 ggatggtcga tgacagatga actggcgatg agatcaattc ttgcagttgc ggatgcttcg
      241 ccagatgccg aggcaatggt ggtaaccggc gccgggacga gaacgctgga tatcctttcc
      301 gatctggaat caaagatcaa gtgtccgata attgctgccg atacgattct ttactgggca
      361 attgcccgtg agttggatct gacgctgaaa cccgcaatgg gttccttgtc aaagctggca
      421 aaaaaatact gaatcatcga acaacggtat tcacttggat atggcttagg ctcaccttcc
      481 ataacaggtc atactctgta ggagagaaaa ataatgagac atcgcttatg gcagcgactg
      541 accgttcttg tccgttttct tcttatactt ttctttacaa ccggttgtat cctgttcccg
      601 accggaatct gcttcagcgg accaaatgcc gattattcct accggatacc ccccgtgata
      661 gatgacggct ggaaaacgga atctctttat aaaaccacta tcgatgctga taaactcatt
      721 gacctgattc gtcatatccg gaacggcgac tacaagaata tccatggcgt gctcctcgta
      781 aaggacggga aactcatgct cgaggaatat tttcatggtt tcgatcgtga aaaacctcat
      841 cagatccgtt ccgccaccaa atcgatcggc tccatactga ccgggatagc catcgatcat
      901 cgtttcatta aggatataaa ggagaaaata tatccctact tcaaagagta cgacatcgga
      961 caggaatggg atgcgagggt cagggatgta acattggagt cgttattgac catgacgtcg
     1021 ggatatgcct gtaatgatca tgcaacgccg aaattcgagt gtgaaaaggc gatgtacggg
     1081 gccgatgact gggtggagta tgccctcaat ctcccgatgg cccataagcc tggggaacat
     1141 tgggcctata acagcagcag cctcatgttg gtgagtcaaa ttatctcaaa gacatccaca
     1201 atgaccatcc cggattttgc ggagaggtat ctctttgaac cattggggat caatgagttt
     1261 cactgggggt tttccccgaa agggcgggca tggattgccg gaaatgcgaa aatgaaaccg
     1321 agggatatgg cgaagatcgg ccttctcatg ctggagggcg ggaaatggaa gggcaaacaa
     1381 attgtctcag aaaaatggat aggagaatcg accagggagc atgagaaatc agaaaataac
     1441 tggggctacg ggtatctgtg gtgggtgggc gaacagctaa tcggtgagca aattattacc
     1501 ggatactggg cggccggtaa tggtggaaat tatatattcg tctgtccggg attggacctg
     1561 gtggcagttt tcacgggggg aaattataat agtatcctgg aattacagac cttgggaatg
     1621 ctgatcaatt acatcattcc cgccatgctg ccgccgattt tgccccggga gattataaaa
     1681 cttgatccgg cgattcttga ctcctgcgtt ggtgactacc agttgcggca gggtgatatt
     1741 cgcgtatcgg tctttagaaa gagcgataca ctttattgca caatactgga aagaacaatg
     1801 cggatgtatc ctgaaacgga tgatcttttt tttatccccg atgacatact gggtaactgg
     1861 acatttaaaa tcgagagaaa tgaaaagggt gacgtgacct ccgcgacagg ttataccgcc
     1921 tttcaggcc
//
LOCUS       DSEB01000348            2403 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1006759, whole genome shotgun sequence.
ACCESSION   DSEB01000348 DSEB01000000
VERSION     DSEB01000348.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1006759"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..414)
                     /locus_tag="ENO07_06215"
     CDS             complement(<1..414)
                     /locus_tag="ENO07_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01597.1"
                     /translation="MRTLDFEYFKNTVNAMRVDRIIGRPIGIIGLARSGLAAAKLLKR
                     LGGDPFVSDVRTGSELKKELAELREVNVPYETGGHTQKLLESSEFLIVSPGVPNDIPI
                     LQSAQEIGIPVFSELEVASWLCDANLAAVTGSNGKT"
     gene            complement(375..1460)
                     /locus_tag="ENO07_06220"
     CDS             complement(375..1460)
                     /locus_tag="ENO07_06220"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDS01598.1"
                     /translation="MFYHLFSSLVDSFSYFNLFRYITFRTAAATITALLVSLLLGPLI
                     VKLLQKKTVTEKIREEGPRSHYAKEGTPTMGGIIILASMIIPTLLWADLSNRFVQMII
                     LVTVWLGILGFMDDFMKAILKKPKGMVGKYKLAGQITLGIVFGGILYFFPPTENFNGY
                     TEIPFFKDYILNFGWLFIPFVILVLTASSNAVNLTDGLDGLAIGLCMMVFLTFAGFSY
                     VTGRADFSGYLDISYLEGAGELTIYCGAAIGAGLGFLWYNSHPAQVFMGDTGALALGG
                     ALGAIAIMLKKELLLVIVGGVFVAEALSVILQVASYRLRGKRIFKMAPLHHHFELSGW
                     PEAKVVVRFWILGALCALLTLSTLKIR"
     gene            complement(1460..>2403)
                     /locus_tag="ENO07_06225"
     CDS             complement(1460..>2403)
                     /locus_tag="ENO07_06225"
                     /inference="COORDINATES: protein
                     motif:HMM:PF02875.19,HMM:PF08245.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDS01599.1"
                     /translation="LSLALLNDSYEICVLEFGMSFPGEIEALTSMVKPQVALITNIGP
                     AHLETMGTIENIARAKFELLDHIDADSIRVLNLDDALLKARFDKEPEPKLGYAVRADA
                     QVRPTGFSSNSLGRIIFQYQGGEIHLKVPGLHTLYNALASCAVARIFEVEFEGVRTAL
                     QSYKGKSSRMEILELSGITVIDDSYNANPTSMGYALKALSQMQAKGRKIAILGDMREL
                     GEDEIELHHDVGSIVAEINPDRLITAGRLGMHIAAGANVNGYDSSRTETFMTAEEVIE
                     AMLPEIRDGDQILIKASRAMQFEKIVRAFKKKQEVRS"
BASE COUNT          593 a          650 c          574 g          586 t
ORIGIN      
        1 agtctttcca ttggaacccg ttaccgccgc caggttagcg tcacagagcc atgaagccac
       61 ctccagctcc gagaagacag gaatgccgat ttcctgggcg ctctggagta tggggatgtc
      121 attcggaacg ccgggtgaga ctattagaaa ttcagagctc tccaggagct tctgtgtgtg
      181 gcccccggtc tcatagggaa cattcacttc tctgagttca gccagctctt ttttcagttc
      241 tgatccggtc ctgacatcag agacaaaggg atcgccgccc agccgcttca acagctttgc
      301 ggcagccaga cccgaacgtg ccagcccgat gattcctatc ggcctgccta ttattctgtc
      361 cacacgcata gcgtttaccg tatttttaaa gtactcaaag tcaagagtgc gcataaagct
      421 ccaagaatcc agaacctgac gacgaccttt gcttcgggcc accccgataa ttcaaaatga
      481 tgatggagag gagccatttt aaagatcctc tttccccgca atctataaga ggctacctgc
      541 agaatgacag agagcgcttc tgcaacgaag actcctccca caatcaccag taataattct
      601 tttttaagca taattgctat ggctcccagc gctcctccca gagcgagtgc gccggtgtca
      661 cccataaaga cctgggccgg atgcgaattg taccacaaga acccgagccc cgcgccgata
      721 gctgcgccgc aatatatggt cagctctccc gctccctcga gataactgat gtccagataa
      781 ccggagaagt cggcgcggcc ggtaacatac gaaaatcccg caaatgtcag aaaaaccatc
      841 atacacagac caatagccag gccatcgaga ccatcagtta aattgacggc gttcgatgag
      901 gcggtcagga ccaggatcac aaaaggaata aacagccagc cgaagttgag gatgtagtct
      961 ttgaagaacg gtatttcagt atagccgtta aaattttcag tgggcggaaa gaaatacaat
     1021 atgccgccga agacgatgcc gagcgtgatc tggcccgcga gtttgtattt tccgaccatg
     1081 cccttgggct ttttcagaat tgctttcatg aagtcatcca tgaatcccag aattcccagc
     1141 cagaccgtaa ccagaatgat catctggaca aaacggtttg acagatcggc ccagagcaaa
     1201 gtggggatta tcatagatgc caggattatt attcctccca tggtcggggt accctctttg
     1261 gcgtaatggc tcctgggtcc ctcctcgcgt attttttccg tcacagtttt cttttgaaga
     1321 agctttacga tcagcggacc caggagaaga gatacaagca gggccgtgat agtggcagcc
     1381 gctgtcctga aggtaatata cctgaacagg ttgaaatagc tgaatgagtc aaccagggaa
     1441 ctgaacaggt ggtaaaacat cagcttctga cctcctgttt ctttttgaag gcccgcacaa
     1501 ttttctcaaa ctgcatggcg cgtgacgctt taatcaatat ttgatccccg tcgcgtatct
     1561 ccggcagcat ggcctcaatt acttcctcgg cagtcatgaa tgtctctgtc ctgcttgaat
     1621 cgtagccgtt gacattggcc cccgccgcta tatgcatgcc cagccttccc gcagtaatca
     1681 gccggtcagg attgatttca gcgacgatgc tgccaacgtc atgatgcagt tctatctcat
     1741 cttcgccaag ctcccgcatg tcacccagta tggctatctt ccgtccctta gcctgcattt
     1801 gagagagcgc cttgagagca tatcccatgg aggtcgggtt ggcgttatag ctgtcgtcga
     1861 tgacagtaat cccgctcaat tcgagaatct ccatgcgcga gcttttacct ttgtagctct
     1921 ggagtgcagt cctgacaccc tcgaactcga cttcgaatat ccttgcaacg gcgcaggacg
     1981 ccagggcatt ataaagggta tgcagtccgg gtactttcag atgtatttcc ccgccttgat
     2041 actgaaaaat aatccttccc aggctgttgg atgaaaatcc tgttggtctg acctgggcgt
     2101 cagcccgaac tgcataacca agtttgggct ccggttcctt gtcgaaccgg gcctttaata
     2161 gagcatcatc cagattcagc acgcggattg aatctgcgtc tatatgatcc agaagctcga
     2221 attttgctct ggcgatattt tcaatcgtac ccatcgtttc cagatgggcc gggccgatat
     2281 tggtgatcaa tgcgacctgc ggcttaacca tgctagtcag tgcctctatc tcacccggaa
     2341 agctcatgcc aaattcgagc acgcagatct catagctatc attgaggagc gccagcgaga
     2401 gag
//
LOCUS       DSEB01000349            3013 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004084, whole genome shotgun sequence.
ACCESSION   DSEB01000349 DSEB01000000
VERSION     DSEB01000349.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004084"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1199
                     /locus_tag="ENO07_06230"
     CDS             <1..1199
                     /locus_tag="ENO07_06230"
                     /inference="COORDINATES: protein motif:HMM:PF01520.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDS01600.1"
                     /translation="SLRNITYPILYQDADFYVPAITSVPVIADLAGITMVWDANELSI
                     RAAELQHNVLDLKFSPRSNGYLCEIILAHPLEYEVLVTEGSWINITLQGGNLNPGYIS
                     NRPHSSVIRKIRAFQFESSSQISINFRRDVNEMHHSLALNPPRIQISIIDTAFDYSAI
                     DTIFSDDEFDPIDVIVIDAGHGGAEDGAIGPKGTLEKEIVLDIALRLEKLFEKDDVRV
                     VLTRRDDTTLLLDERANLANSSGGEMFVSIHANWFEDGAAKGSQTFFLAAALNDAARA
                     TAMLENKSILLNEEGEITDTADELDLILFDLLQTEYLAESQALAREIQAEMIKNLRTR
                     NRGVDQAGFFVLNKVYMPSVLVEVAFISNKHEEALLGKESFRQKAAEGIYAGIKNFID
                     KYSPDR"
     gene            1208..2026
                     /locus_tag="ENO07_06235"
     CDS             1208..2026
                     /locus_tag="ENO07_06235"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDS01601.1"
                     /translation="MANIDSSQPIGIFDSGLGGLTVVKEILKILPNEDIIYFGDTGRT
                     PYGPRSKDIIIEFSRQDTNFLLEQNVKIIVVACNTASAQALDEIRKDYDIEMIGVIEP
                     GAISAVRATKNGKVGIIGTAGTISSDSYARTINRLDSSIKVFSMACPLFVPLVEEGYL
                     DKEATRLIAEDYLSIFTSNGIDTLVLGCTHYPLLKGIIHEILGRDIVLIDSAEETAQI
                     VYKKLIEMNLLREKTASVLHKFYVSDFPERFHQVARHFMGDRIQNVIRIDINKY"
     gene            2053..2667
                     /locus_tag="ENO07_06240"
     CDS             2053..2667
                     /locus_tag="ENO07_06240"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HDS01602.1"
                     /translation="MNQIVLATNNEHKIREISFILRDLPLSILSKKDFSDFPDVEETG
                     QTLEENAILKARAIFEATNIISIADDSGLEVAALGGRPGVNSARYAGPGCSFEDNNRK
                     LLKEMEGVPESQREALFRCVIAISFRPDDVELVEGRVTGKITTEIRGLQGFGYDPVFF
                     LPFYNKTFAELPPEEKNRISHRAIAINKARELLIKRLNLRVQNE"
     gene            complement(2746..3003)
                     /locus_tag="ENO07_06245"
     CDS             complement(2746..3003)
                     /locus_tag="ENO07_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01603.1"
                     /translation="MNGRMYDYGWITDKNSGAVIWEMTYMKTEHAGGALKNRLYDGVI
                     MLDKGTYEVYYVTDDSHSYAEWNDYAPPEPIDWGISLRFEK"
BASE COUNT          804 a          771 c          722 g          716 t
ORIGIN      
        1 acagtctccg caatatcact tatccaatcc tgtatcaaga tgcggatttc tatgttcccg
       61 ccatcacctc cgtaccggtg atagcggacc tggccggaat taccatggtc tgggatgcta
      121 atgagctctc catccgggcc gcggaactgc agcataatgt tctcgatctt aaattttctc
      181 ccaggagcaa cggttacctg tgtgagatca tcctcgctca cccgctcgaa tacgaggtcc
      241 ttgtaaccga gggaagctgg atcaatatta ccctgcaggg cggaaattta aatccgggct
      301 atatctcaaa ccggccccac agcagtgtta tcagaaaaat ccgcgctttt cagttcgaaa
      361 gctcttcgca gatttccata aatttcaggc gtgatgtaaa tgaaatgcac cacagcctgg
      421 ccttaaatcc gcctcgtata cagatatcca taatcgatac cgcttttgac tactctgcaa
      481 tcgacactat cttctcggat gatgaatttg atccgatcga tgtcattgtc atcgatgccg
      541 ggcacggcgg cgccgaggat ggcgctatcg gccccaaagg aactctggaa aaggagattg
      601 tactggacat cgctctgcgg ctggaaaagt tgtttgaaaa ggatgatgtg agagttgttc
      661 tcacgcgtcg ggatgatacg acacttctgc tcgatgaaag agccaacctg gccaacagct
      721 ctggcggaga gatgtttgtg tcgattcatg ccaactggtt tgaggacggt gccgcaaagg
      781 gctcacagac tttcttcctg gccgcggcac tgaacgacgc cgcccgcgcc accgccatgc
      841 tcgaaaataa atccatactt ctaaatgaag agggcgagat aaccgatact gccgatgagc
      901 tcgatctgat tttattcgac ctcctgcaga ccgaatacct ggccgaatca caggccctgg
      961 cgcgcgagat ccaggccgaa atgatcaaga acctgagaac ccgcaaccgc ggggtggacc
     1021 aggccggatt ctttgtgttg aacaaagttt acatgccctc agtccttgtt gaagtggctt
     1081 ttatcagtaa caaacacgag gaggcattgc tcggcaaaga gagtttccgg caaaaagcgg
     1141 ccgagggaat atatgccgga attaaaaatt ttattgacaa atattctccc gataggtaac
     1201 aaaccatatg gcaaatatcg atagttcgca gccgataggc atttttgatt cgggtctggg
     1261 cggcctgaca gttgtcaaag agatcctcaa gattctgccc aacgaggaca taatctattt
     1321 cggcgatacc ggacgcaccc cctatggtcc ccgctccaag gacataatca tcgaattctc
     1381 gcgccaggac acaaatttcc tgctggaaca gaatgtcaag ataattgtgg tcgcctgcaa
     1441 tacagcttct gcacaggccc tggacgagat aagaaaagat tatgatattg aaatgatcgg
     1501 cgtgatcgag ccgggagcga tttccgctgt gagggctact aaaaacggca aggtcggtat
     1561 aatcggaact gccggcacca tctcctccga ttcttatgcc cgtaccataa accgtctcga
     1621 ttccagtatc aaggtgttct ccatggcctg cccccttttc gtacccctgg tggaggaggg
     1681 ttatcttgat aaagaggcca cccgtttgat tgccgaagat tacctgtcca tattcacaag
     1741 caatggtatc gacactctgg tcctgggctg tacgcactac cccctcctga aggggattat
     1801 ccacgagatt ctgggcaggg acatcgtctt gatagattcg gcggaagaaa ctgcacagat
     1861 agtgtataag aaactgatcg aaatgaattt gctcagagag aaaaccgcca gtgtactcca
     1921 taaattctat gtctcggact ttcctgagag gtttcatcag gtagcccggc atttcatggg
     1981 cgacaggatt cagaatgtga tccgcatcga tataaacaaa tactaatcta aaaggggcaa
     2041 tacagagata caatgaatca gatagtactg gcgacaaaca acgagcacaa gatcagggaa
     2101 atctccttta tcctgcggga cctgccgctt tcaatactca gcaaaaagga cttcagtgac
     2161 tttccggatg tcgaagaaac cggccagacc ctcgaggaaa atgccatcct taaggccagg
     2221 gccatatttg aagccacaaa tataatttcc attgctgacg attccggact ggaggtggcc
     2281 gctctgggag gcaggccggg tgtaaattcc gcccgttacg ccggcccggg atgcagtttt
     2341 gaggacaata acagaaagct cttgaaggaa atggaaggtg tgcccgaaag ccaacgggag
     2401 gcattattca gatgtgtcat tgctatctct ttcaggccgg atgatgtcga gcttgttgag
     2461 ggccgggtga caggaaagat cacgaccgaa atcaggggcc tgcagggctt cgggtatgac
     2521 cctgtattct ttctaccctt ctacaacaag actttcgcag aacttccccc cgaagagaag
     2581 aacaggatct cgcaccgcgc catagctata aacaaggccc gggaacttct aataaaaaga
     2641 ctgaacctcc gtgttcaaaa tgaataactg cgaaactggt cccgctaaat aaaaaatgcc
     2701 gctctctccg gcggctcatt ttttatgcac gaaaaaaatc aagctctact tttcaaatct
     2761 caatgatatg ccccagtcaa tcggttccgg gggagcatag tcgttccatt ctgcatatga
     2821 atgcgagtca tcggtaacgt aataaacctc ataggtcccc ttatcgagca taatcacgcc
     2881 gtcatacagc ctgtttttca gcgcaccgcc ggcgtgttct gttttcatat aggtcatttc
     2941 ccagatgact gcgccgctgt tcttatcggt aatccagccg taatcataca tcctgccatt
     3001 cacgccctct ccc
//
LOCUS       DSEB01000350            1933 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1010891, whole genome shotgun sequence.
ACCESSION   DSEB01000350 DSEB01000000
VERSION     DSEB01000350.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1010891"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..290
                     /locus_tag="ENO07_06250"
     CDS             <1..290
                     /locus_tag="ENO07_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDS01604.1"
                     /translation="FKDTIIAKHTISPAAFESYNPNYIGGDINGGVQDLGQLFFRPVP
                     SLSPYRMPLKGHYICSSSTPPGGGVHGMCGYHAARRALKDIFDIHFDISKT"
     gene            complement(359..>1933)
                     /locus_tag="ENO07_06255"
     CDS             complement(359..>1933)
                     /locus_tag="ENO07_06255"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005003796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HDS01605.1"
                     /translation="AGSLGMLAMGAGGLEVATAMAGEPFYVKMPRIMGVKLTGKMPDW
                     VSAKDVILEMLRRHGVSGGVGKIIEYYGPGLDSLSAMDRHVIANMGAELGATTTVFPS
                     DNNTRIFLEQQERADVWTELVHDDGAEYDEHDEIDLSALEPLIAKPSSPGNVVPVTEV
                     AGKEIYQAMVGSSANPGFRDFAIAALIVDGKQVHNRVSFDINPTSRQITENLIELGYL
                     GKLIRSGGRVHQPGCNGCIGMGQAPATGKISLRTVPRNFPGRSGTKEDSVYLCSPETA
                     AASALTGVITDPRELGMEYPAYSEPDKIKINTSMLLAPAEKSEDVELEKGPNIKTLPE
                     LDPIPDNIQGPVLMKAGDDVSTDEIMPAGTKVLPLRSNIPEISKFTFAQIDDKYYQRA
                     MKYKDSGSFVVGGSNYGQGSSREHAVLGPRYLGVKVVLVKSFARIHWQNLINFGILPL
                     TFKNPAAYDRIGMEDVLVFRNIRKSIQDGGEIEVENKTKGEKFTAVHNMSERQVEMIL
                     KGSLINIFRERNKANS"
BASE COUNT          455 a          535 c          454 g          489 t
ORIGIN      
        1 gatttaaaga tacaataata gcaaaacaca ccatctcacc agctgctttc gagagctata
       61 atccgaatta tatcggcggt gatattaacg gcggagtcca ggatctgggg caattatttt
      121 ttcgtcctgt gccgagctta tcgccataca ggatgccgct taagggccat tatatctgct
      181 cctcgtcaac accgccggga ggcggggtgc atggcatgtg cggttatcat gccgcccgcc
      241 gcgcattaaa agacatattc gatattcatt tcgatatcag caaaacataa aagagaattc
      301 ccgacccaaa tcgcagaaat tcgagccggg aatatcacgc agaattgcgt atgggatttc
      361 aactattcgc cttatttctc tcgcggaata tattaatcaa acttcctttc aaaatcatct
      421 ccacctggcg ctccgacata ttatggacgg ccgtgaattt ttcacccttc gtcttgttct
      481 ccacctcaat ctcgccgccg tcctgaatcg atttacgaat attcctgaaa accagaacat
      541 cttccattcc gattctgtca taggcagcgg gatttttgaa tgtcagcgga agaatgccga
      601 aattaatcag gttctgccag tggatacgtg caaagctctt gaccaatacg accttgacgc
      661 ctaaataccg cggtcccaaa acagcatgct cacggcttga gccctggcca taattgcttc
      721 cgccaaccac aaatgatccg ctgtccttat acttcatagc gcgctgataa tatttatcat
      781 caatctgggc gaatgtgaac ttgctgatct cgggaatatt acttctaagc ggtaaaactt
      841 tggtacccgc gggcatgatc tcatcggtag agacatcatc cccggctttc ataagaaccg
      901 gcccctggat gttatcggga atcgggtcga gttcaggtaa tgttttgata ttgggaccct
      961 tttcaagctc gacatcttcg cttttctcgg ccggggcaag cagcatcgat gtatttatct
     1021 taatcttatc cggctcggaa tatgcgggat attccatgcc gagttcacgc ggatctgtaa
     1081 tcacgcctgt caaagcggag gctgcagcgg tttccgggct gcacagataa accgagtcct
     1141 cttttgtacc ggaacgtccg gggaaatttc tcggcacggt gcgcaggctt atcttgccgg
     1201 tggcgggcgc ctgtcccatc ccgatacatc cattacatcc gggctgatgg acccttccgc
     1261 ccgaccggat cagcttcccc agatatccca gttcaatcag gttctctgtt atctggcgag
     1321 aggttggatt tatgtcaaag gacacacgat tatgaacctg tttgccgtca acaataagcg
     1381 ccgcaatggc gaagtcacga aagccgggat tggccgatga tccgaccatc gcctggtaga
     1441 tttcttttcc ggccacttcc gtgaccggca caacattgcc gggactgctg ggtttggcta
     1501 tcaacggctc aagggcggag aggtcgatct catcatgctc atcatattca gcgccgtcat
     1561 cgtggacaag ctcggtccag acgtcggccc gttcctgctg ttcgagaaat attctggtat
     1621 tattgtctga cggaaaaaca gtggtggtgg cgcccagctc ggcgcccata tttgcaataa
     1681 catgacgatc catagcgctc aggctatcca gcccggggcc gtaatattcg attatttttc
     1741 caacaccgcc ggagacccca tgacgcctga gcatctccag aataacatcc ttggcgctga
     1801 cccagtccgg catttttccg gtgagtttga cgcccatgat cctgggcatc ttgacataaa
     1861 acggctcccc ggccatggcg gtcgccactt caaggccgcc cgcacccata gccagcatcc
     1921 ccagcgatcc ggc
//
LOCUS       DSEB01000351            2102 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1009042, whole genome shotgun sequence.
ACCESSION   DSEB01000351 DSEB01000000
VERSION     DSEB01000351.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1009042"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            138..863
                     /gene="trmD"
                     /locus_tag="ENO07_06260"
     CDS             138..863
                     /gene="trmD"
                     /locus_tag="ENO07_06260"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003145881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDS01606.1"
                     /translation="MRIEVLTIFPEIFDSPIKSSLLGKARDAGLLEINVSDIRDFSTD
                     KHRTVDDTPFGGGAGMVMKAEPIDRALQQIRENLTDPRLLLTSASGKKFDQKMANELS
                     REESIIIICGRYKGVDERILELHPIEEVSIGDYVLSGGEAAALVMIEAVARLVPGYMG
                     KIEAGEDDAFAWGILGFPNYTHPQEYKGLKVPDVLVSGHHAKIERYRRYSALKKTLQN
                     RPELLETIELSDIDKKLLDEIKK"
     gene            903..1283
                     /locus_tag="ENO07_06265"
     CDS             903..1283
                     /locus_tag="ENO07_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDS01607.1"
                     /translation="MDLMSKLEGEYRKKDVPKFSPGDTVKVHVRIKEGDKERIQVFQG
                     TVIKKRGSGLGASFTVRKVSSGIPVERVFPIHSPNVTRIERLRMGKVRRAKLYYLRNL
                     SGKQSRIVEKKEDKKTNNKRANEK"
BASE COUNT          592 a          480 c          539 g          491 t
ORIGIN      
        1 accaatgaca tattggtaat acgttccgac cggggaaggc ttctgattcc ggcaataaaa
       61 gagatagtca ggaaagtaga tcttaaaaac aacaaactgg aggtaacgct tctacccggg
      121 atggaatttg aggctgaatg agaatcgagg tcttgaccat ttttccggaa atcttcgaca
      181 gtccgattaa atcaagtctg ctgggaaagg cccgggatgc cggtcttctg gaaatcaatg
      241 tgagcgacat tcgggatttt tccacagaca aacacaggac agtcgatgat actcccttcg
      301 gcgggggagc gggaatggtc atgaaggccg aaccgatcga cagagcgctg caacagatca
      361 gggaaaatct cacggacccg cggctgcttt tgacctcggc ctcaggaaaa aaatttgatc
      421 agaaaatggc caatgaattg tcccgggaag agagtattat aataatctgc ggaagatata
      481 aaggcgtgga tgagcgcatt ctcgagctgc acccgattga agaggtctcc atcggcgact
      541 atgtcctttc cggcggggag gccgctgcgc tggtcatgat cgaggcggtc gcgaggctgg
      601 taccgggata tatgggcaag atagaagcgg gcgaggatga tgccttcgcc tggggaattc
      661 tgggttttcc aaattatact catccgcagg aatacaaggg cttgaaagtt ccggatgtcc
      721 tggtctcggg gcatcatgcg aaaatcgaga gatacaggag atacagcgcc cttaaaaaga
      781 ctttgcaaaa tcggccggaa cttttggaaa caatcgaact tagtgatata gataaaaagc
      841 ttttggatga aataaaaaaa tgaggaaaaa taactctgca aatagatttc tggaggtctg
      901 aaatggactt gatgtcgaaa ctcgaaggtg agtacaggaa gaaagatgtg ccgaaattct
      961 cccccggaga tactgttaag gtccacgtcc gcattaagga aggcgataag gagagaattc
     1021 aggtgtttca gggcacggta atcaagaaac gcggttccgg cctgggagca tcatttacgg
     1081 tcagaaaagt ttcgtccggt attccggtgg aacgagtatt ccccattcat tcgccgaatg
     1141 ttaccaggat tgagaggctt cggatgggta aggttagacg tgcaaaactc tattaccttc
     1201 gcaacctttc cggaaaacaa tcccggatag tcgagaagaa ggaagacaaa aagacaaaca
     1261 ataaaagagc gaacgagaag taattaccgc cgttcgcaaa atagtcaaat aaaaagcccg
     1321 ctcgcgggct tttttattcc tgtttgaaat tatcgaggtc gagcaggaga ttgccgaccc
     1381 ggaaagtctg gttcatcctg gcatacacca gcccctcatc atcgcgcaat tccagtatat
     1441 gataacccgg ttccagtccc agctggtctt tcataaactg cgagacagta ccctgcggta
     1501 tcatatccac atataaggtg tgatcgaaga tggaatcggg aaaattatga aatttaatga
     1561 ataccgtgcc cacagcctcg gcttgagcac cgacattttc attattagaa ccggtcaccc
     1621 ggctcataaa ttcatccacc ggcggcctga ccccgccgat attataggca tatgcgaggt
     1681 aagccaggat tgcaatcaat acaataaatg aaagcacata ccaggcaaag gcgcccttgc
     1741 tttcgggttt gtcccgataa acgggaaccg gtcctgttat ctgctcaccg ggagatttgc
     1801 tgtcttcggt ttcctgcctg gaggcgaatg cctttcgcag gatatcgtct tcaatcttta
     1861 gctcctggct cgtttcctcg tccacagtct gctgtaccag atccttggaa aaatcgtccg
     1921 ggctcttgat atccagaacc ttaactacag caacagtaat attgtcttcg cccccgctgg
     1981 cattggcgcg ttctatcagc tctttgcaga ttttgtcgag gctggcattt tccctggata
     2041 gcgtctggag aatattcgca tcggaaacaa atccgctcag gccgtccgag cacagcaggt
     2101 ac
//
LOCUS       DSEB01000352            2404 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1006753, whole genome shotgun sequence.
ACCESSION   DSEB01000352 DSEB01000000
VERSION     DSEB01000352.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1006753"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1471
                     /gene="uvrB"
                     /locus_tag="ENO07_06270"
     CDS             <1..1471
                     /gene="uvrB"
                     /locus_tag="ENO07_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDS01608.1"
                     /translation="HYSRNDIDFSRGTFRVRGDSVEIFPAYEVFPYRVEFFGDEVERI
                     VEIDHVSGKIRGEQPGLAVYPAKHFITSPRQLEAAIENIEKELDERLYELRSQNKLLE
                     AQRLESRTNFDIEMMREIGYCSGIENYSRHLSGRAAGEPPYTLIDFFPDDFLLIIDES
                     HQTIPQIRGMYAGDRSRKTVLVEYGFRLPSALDNRPLVFDEFEGHINKCIFISATPDE
                     YELERSEGVVTEQIIRPTGLVDPEIVVKPLATQVDDLIEEIRKRVTKKERILVTTLTK
                     RMAEDLTDYLKSLNIKVRYLHSEIDAIDRVEIIRDLRLAEFDVLVGINLLREGLDLPE
                     VSLVAILDADKEGFLRSEKSLIQTAGRAARNVSGQVILYADKITGSMQRAIDETNRRR
                     AKQLEYNEKHDITPATIFKSRDDIMRTTLFADSRTKEEEQILLPEFFKHLEHSAKINF
                     LKQSMKKAADNLNFEEATFYRDKLRELEKDHRTKSGQRR"
     gene            1488..2363
                     /locus_tag="ENO07_06275"
     CDS             1488..2363
                     /locus_tag="ENO07_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01609.1"
                     /translation="MKSFIFPIALIALAFLIAFGCDRSVDPPYTNPAAIIDLLKVDDI
                     AQEVEDFDFIDTGSYTTLADAQSGDFGTVQYKLEIDSALLTYRVDMGDTVIIDAIRAQ
                     EANAYSQYQIFYRLTLKNETGDSVVKHLVTDSTRARKRHYTLKLGESTQPFRGWVYWG
                     TNMIFNLSQTQPNIKWFSAEQGELPNSSEIIRKNEIPALKPGDRITVQYQGLPSDIGY
                     LHINETGTPRRIKFNRINDVIKEAEWTVSSNYQGRDYFYYAGIEIYRRNTLASADTSE
                     TDFLFMGLMYPISDQ"
BASE COUNT          678 a          564 c          615 g          547 t
ORIGIN      
        1 ccattattca cgcaatgata tcgatttcag ccgcgggacc ttccgcgtac gcggtgacag
       61 cgtggaaatc ttccccgcct atgaggtctt tccataccgg gttgagtttt ttggagatga
      121 ggtcgagagg attgtcgaaa tcgaccatgt cagcggtaaa atacgcgggg aacagcccgg
      181 cctggcagtc tatcccgcca agcatttcat aaccagcccc cggcagctgg aggctgcaat
      241 agaaaatatc gaaaaagagc tggacgagag actgtacgag ctgcgcagcc agaacaaact
      301 gctggaggcc cagcgcctgg aatcacgaac caattttgat atcgaaatga tgcgggagat
      361 aggctattgc agcgggatcg aaaattattc ccggcatctg agcggcagag ccgccggaga
      421 accgccctac acattgatcg acttctttcc tgatgatttc ctgctgataa tcgacgaatc
      481 gcaccagaca attccccaga ttcggggtat gtatgcaggc gacaggtcgc gcaagacagt
      541 tctggtcgag tatggtttca ggcttccctc agcgctggac aatcgccccc tggttttcga
      601 tgaattcgaa gggcatatca acaaatgcat attcatttcg gcgactcccg acgagtatga
      661 gctagagagg tcagaagggg tcgtgacgga gcaaattatt cgtccaacag gactggtcga
      721 tccggaaatt gtcgtcaaac cgctggccac tcaggtggac gatctgattg aggaaatccg
      781 caagcgggtg acaaaaaagg aacgcattct ggtcaccact ctgaccaaac ggatggccga
      841 ggacctgacc gattatctga agagcttgaa tataaaggtc agatatctgc attcggagat
      901 agatgcgatt gacagggtcg agataatccg cgacttacgg ctggcggaat tcgatgtcct
      961 ggtcggaatc aacctcctcc gtgagggcct ggatctgccg gaggtgtcac tggtggccat
     1021 tctggatgcc gacaaagaag gcttcctgcg gtctgagaag tctttaattc aaaccgcggg
     1081 acgcgcggcc agaaatgtct ccggacaggt tatcctgtat gcggacaaga tcacgggatc
     1141 gatgcagcgc gccattgacg aaaccaatcg aagaagggct aagcaattgg aatacaatga
     1201 gaaacatgac atcaccccgg ccacgatttt taaatcgagg gacgacataa tgaggaccac
     1261 cctgtttgca gactcccgca ccaaagagga agagcaaatt cttctgccgg aattcttcaa
     1321 gcatctcgaa cattcggcta aaatcaattt cctcaagcag tccatgaaga aggcggccga
     1381 caatctaaat tttgaggagg ccacattcta tcgtgacaaa ttgcgggaac tggaaaagga
     1441 ccatcgaact aaatccggtc aaagacgtta aatatacagg agttagcatg aagagcttta
     1501 tattcccgat agcattaatc gcactggcat ttctgatcgc atttggatgt gatcgttctg
     1561 tggacccgcc ctacactaat ccggccgcga ttatagattt gctaaaagtg gatgacatcg
     1621 cccaggaagt cgaggatttc gattttattg atacggggtc atatactact ctggccgatg
     1681 ctcaatccgg agatttcggg acagttcaat acaaacttga aattgattcg gctctattga
     1741 catatcgcgt cgatatggga gatactgtga ttatagatgc gatcagggca caggaagcaa
     1801 atgcttactc ccagtatcaa atattctaca ggttgacact taaaaatgaa accggggact
     1861 ctgttgtaaa acatctggtc acagattcga ccagggcaag aaaaagacat tacaccctca
     1921 aactcggaga atccacacag ccctttaggg gatgggttta ctggggtacg aatatgatct
     1981 tcaacctgtc ccagacacag ccgaatatta aatggttctc agcagaacag ggggaattgc
     2041 ccaattcaag tgaaattatc aggaaaaacg agattccggc gctcaagccc ggcgaccgta
     2101 tcacagtgca gtaccaggga ctgccgagcg atataggtta ccttcacatc aatgaaaccg
     2161 gaacccccag gaggattaag ttcaacagga tcaatgacgt aatcaaggaa gccgaatgga
     2221 cggtttccag caattatcag ggcagggatt acttttatta tgccgggatc gagatctacc
     2281 gcaggaatac cctggcgtct gcggatacct ctgagacgga ctttttgttc atgggcttga
     2341 tgtatcctat cagcgatcaa taaaattgtg ggaactttcc ggattatttc gtatattata
     2401 aggc
//
LOCUS       DSEB01000353            2523 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1006051, whole genome shotgun sequence.
ACCESSION   DSEB01000353 DSEB01000000
VERSION     DSEB01000353.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1006051"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..630
                     /locus_tag="ENO07_06280"
     CDS             <1..630
                     /locus_tag="ENO07_06280"
                     /inference="COORDINATES: protein motif:HMM:PF00795.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDS01610.1"
                     /translation="EEIPEGRTTKLFISLARQKKCAFVIGIAEKKDGRCFNSSVYITP
                     AGDVHLYRKVHLFYKEQFIFNRGNLKFKAFPFYEYRLGMMICFDYIFPEACRVLALRG
                     ANIVCHPSNLVLNYCQITMPIRSIENRIFTVTANRVGSDTVGDETLKFTGMSQITDVD
                     GNVLVRAGKHKPAVAVKDIDLRKSLEKMPTEFNDLIKDRRPDFYEELLR"
     gene            721..1095
                     /locus_tag="ENO07_06285"
     CDS             721..1095
                     /locus_tag="ENO07_06285"
                     /inference="COORDINATES: protein motif:HMM:PF00072.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDS01611.1"
                     /translation="MDREIKILSIDDEPEVLDSLSELLDAAGFDVRSSNDAERALEIA
                     SEFKPDAIVLDIIMPQMDGYQFCKLLRKNPETADIPVIFLTGVDPQDDSGKAFEAGGQ
                     LFVKKPFRIEELVEVIKIAASD"
     gene            1146..1565
                     /locus_tag="ENO07_06290"
     CDS             1146..1565
                     /locus_tag="ENO07_06290"
                     /inference="COORDINATES: protein motif:HMM:PF00072.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDS01612.1"
                     /translation="MMSTKIPIPKQKVKPAKILVIDDEPEITEVIEAFLKSSGYEVVT
                     ENTAIVAVERVLDYKPDLILLDIMMAGMDGYQICEELKKRPDTRNIPVLFLTGKDSRE
                     DSGKSFKVGGDMFIKKPFSGDRLLEIINVVLVSIGKR"
     gene            1630..2196
                     /locus_tag="ENO07_06295"
     CDS             1630..2196
                     /locus_tag="ENO07_06295"
                     /inference="COORDINATES: protein motif:HMM:PF07879.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01613.1"
                     /translation="MYLIKRYSNRRLYDPQANCSITLEDLVEIIRRGNRVKVVDSKSG
                     RDITSRVLGQALLSDMKRWKDTESKSEIIKLIIAEGEGAVDILKKTYLFGLGAFEMTR
                     EKAENLIDEMVKKGEVKKGERADAVIELMDKVDENVKTFRDKVSAEVEDKMSRMRVAR
                     KTDLEALEKKVDSLIETVSKLEEKLGEK"
BASE COUNT          701 a          543 c          660 g          619 t
ORIGIN      
        1 gaagaaattc cggagggcag gaccacgaag ttattcatct ccctggccag gcagaaaaaa
       61 tgtgccttcg ttatcgggat tgccgagaag aaagacggtc gctgtttcaa ttcgtcggtt
      121 tacatcacgc cggccgggga tgtgcatctt tatcgcaagg tccatctatt ttacaaggag
      181 cagtttattt tcaaccgcgg gaatcttaag ttcaaggctt tcccgtttta tgaatacagg
      241 ctgggtatga tgatctgctt cgattatata tttccggaag cctgccgcgt gctggctttg
      301 cggggagcca atattgtctg ccacccctcc aatctggtac ttaattactg tcagataaca
      361 atgcccatcc gttcgataga aaaccgcatc ttcacagtaa ccgcaaatcg cgtcggttca
      421 gatacggtcg gagatgagac ccttaaattt accggaatga gccagatcac tgacgttgac
      481 ggtaacgtcc tggtgcgagc cggaaaacac aaacccgccg tggcggtcaa agatattgac
      541 ctgcgcaaga gcctggagaa gatgcccacc gagttcaacg atctcatcaa agacaggcgt
      601 ccggacttct acgaggaatt gctgcgctga ttaattctcc cggcagcatt cttttttgtc
      661 cacatcgcta cattttgaag ctggttttcc gatagaaaca gtgagaggat gtagtgtttt
      721 atggatagag agataaagat attatcgatt gacgacgagc cggaagtgct ggattccctg
      781 tctgagctcc tggatgccgc agggtttgat gtcagatcat ccaatgatgc cgaacgggcg
      841 cttgaaatcg cgagcgagtt caagcccgat gcaattgtat tggacataat tatgccccag
      901 atggatggct atcaattctg caaattgctg cgaaagaatc ccgagacggc cgatattccc
      961 gtaatttttc tgacaggtgt cgatcctcag gatgactcgg ggaaggcttt tgaggccggc
     1021 ggtcagcttt ttgtaaagaa accgttccgg atcgaagagc tggtcgaagt gatcaaaatt
     1081 gctgcctcag attaggatac ctgaagttgc gttaaagcgg gctctgcaaa aggccgataa
     1141 tatcagtgat gagtacaaaa atacccattc ccaagcagaa ggtcaaaccg gccaaaatac
     1201 tggttatcga tgacgagccg gaaatcaccg aggtcatcga agcgttcctg aaatcctctg
     1261 gctacgaagt ggttacggag aatactgcaa tcgtggctgt ggaacgggtg ctggattata
     1321 agccggacct gatacttctc gatattatga tggcaggtat ggatgggtac cagatctgcg
     1381 aggaactgaa aaaaaggccg gataccagaa acatcccggt cctgttcctg accggaaaag
     1441 attcccgtga agattcaggg aaatccttca aggtgggcgg agatatgttc atcaaaaaac
     1501 ctttctccgg cgatcgcctg ctcgaaatca tcaatgtcgt cctcgtttcc atcggaaaac
     1561 gctgacccat aatatttcca gcccgcattt ttttattgac atttatggtg caatgcacta
     1621 aaatacaata tgtatctgat taagcggtat tcaaaccggc gcttatatga tccccaggcc
     1681 aattgctcga ttacgctcga ggatctggtt gagattatca gaaggggcaa tcgggtcaag
     1741 gtggtcgatt ccaaaagcgg acgcgatatc acctcgcggg tgctggggca ggcattgtta
     1801 tcggatatga agcgctggaa ggataccgag tcaaaatccg agataatcaa acttatcata
     1861 gcggaaggag aaggcgctgt ggatatttta aagaaaactt acctgtttgg tctgggggcg
     1921 ttcgagatga cccgggagaa ggccgagaat ctgatcgatg agatggtcaa gaagggagag
     1981 gtcaaaaaag gcgaacgtgc cgatgcggtc atcgagctga tggacaaagt ggatgagaat
     2041 gtcaagacct tcagggacaa ggtttcggcc gaggtcgagg acaagatgag caggatgcgg
     2101 gtggcaagaa agaccgacct ggaagctctg gagaaaaaag ttgacagcct gatcgaaaca
     2161 gtctccaagc tggaggagaa actgggcgag aagtaaatag cgcatatctg tcagaactct
     2221 gcacagattt taaccggatg tgcacatatc atcatgataa aggtctaaat cgcctccgat
     2281 cagactccgt ccggatagct ctaactcgtt agaatatatt aagttatgtg ctttcaggcc
     2341 ggaaaccccg ggttttggcc cttttcttgc aatgtttaac ttgcgaaaac acgtatatat
     2401 tgggaactcc cggaggtttt tgggttttaa tcagtaacta aaacttcttg acaattgggg
     2461 agttatccaa taaattccca ctcgtaattt gaaagagagg agtcgtatag agtaatcgtt
     2521 aaa
//
LOCUS       DSEB01000354            2334 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1007194, whole genome shotgun sequence.
ACCESSION   DSEB01000354 DSEB01000000
VERSION     DSEB01000354.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1007194"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..210)
                     /locus_tag="ENO07_06300"
     CDS             complement(<1..210)
                     /locus_tag="ENO07_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006104527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01614.1"
                     /translation="MDYEGHEYNFLGLEKEKSDYANSRFVILPVPYEQTTTYRKGACN
                     GPHAMISASREVELFDDELKFEAYLE"
     gene            complement(226..1266)
                     /locus_tag="ENO07_06305"
     CDS             complement(226..1266)
                     /locus_tag="ENO07_06305"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014405201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HDS01615.1"
                     /translation="MLKDYLSKPTTPFQVDKSSNVAGLLDNMKNISFQGRTLAQAFAV
                     WKNMLREDTLIFMGLSGAMVPAGMREIIVYLIKNRFIDCLVSTGANLFHDAHESLGNY
                     HYIGSQHVNDAELYEKKVDRIYDTFANENEFRKLERLIDNWAHANLDLSRAYTTREFF
                     YRWGEYLNSIKQADGIVTSAYESGVPIYCPSIADSSYGIALAERFKRNGKVVVFDVIG
                     DVEETALLAARAPQTGVIYLGGGVPKNFIQQAEVTAIIHLGFDSPGHKFAIQIITDAP
                     HWGGLSGCTFSEAESWGKINGDALTATCYCDVTIALPIIATALAQDGEETARKRNKPA
                     FDLGRKLEFSFK"
     gene            complement(1299..1841)
                     /locus_tag="ENO07_06310"
     CDS             complement(1299..1841)
                     /locus_tag="ENO07_06310"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HDS01616.1"
                     /translation="MTPKKLFLTKGVGIHKEKLNSFELALRNAEIAKYNLVAVSSIFP
                     PHAKIISRKEGLKLLRPGQIVYTVISKNSTNEPNRLISASVGIAIPQDRKQYGYLSEH
                     HAYGWTGRHAGEYAEDMAVDMLATTLGLEINLDKSWDENKEIWKVQGKIIRTRNITQS
                     AKGHKEGLWTTVVAAAILIM"
     gene            2031..>2334
                     /locus_tag="ENO07_06315"
     CDS             2031..>2334
                     /locus_tag="ENO07_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HDS01617.1"
                     /translation="MITVKAFRGLRPKEEEASKIASPPYDVLSSQEAREMARENPISF
                     LHINKPEIDLPEDTDAYSDSVYQKGRENLKRLMAEGHLIQDDKESIYIYRQTWRDHV"
BASE COUNT          579 a          563 c          558 g          634 t
ORIGIN      
        1 ctccagatac gcctcgaatt tcagttcgtc gtcaaaaagc tctacctccc gcgaagcgga
       61 aatcatggca tgcgggccgt tacaggcgcc tttcctgtat gtagtggttt gttcgtaggg
      121 aaccggcagg attacaaagc gggagtttgc atagtcggat ttctcctttt ccagccctag
      181 aaaattatat tcgtgtcctt cgtaatccat agatgaagat ccattttatt taaagctgaa
      241 ttccagcttc ctgcccagat cgaaggccgg tttattgcgc ttgcgggctg tttcctcgcc
      301 gtcctgggca agcgctgtgg cgattattgg caaggcaatg gtaacatcac agtaacaagt
      361 tgcagtcagc gcatcgccat tgattttgcc ccatgattcc gcctcggaga atgtgcatcc
      421 ggaaagcccg ccccagtgag gagcatcggt gataatctgg attgcaaatt tatgtccggg
      481 agaatcgaag ccgagatgta taatagcggt aacctccgcc tgctggatga aatttttagg
      541 tacacctccg cccagatata taactcctgt ctgcggcgct ctggcggcca gcaaggcagt
      601 ctcttccaca tccccgataa catcaaaaac aactactttc ccgttacgtt taaacctctc
      661 cgccaaagca attccataag atgagtcggc tattgagggg cagtatatcg gcaccccgga
      721 ttcataggcc gaggtgacta tgccgtcggc ctgcttgata ctgttaaggt attcacccca
      781 gcgatagaaa aattcgcgtg tcgtataagc acgtgataga tcgagattag catgtgccca
      841 gttgtcaatc agcctctcca gcttgcgaaa ttcattctca ttggcgaagg tatcgtaaat
      901 ccggtccact tttttttcgt aaagctcagc gtcattcaca tgctggctgc ctatataatg
      961 gtagtttccc agcgattcat gggcatcatg gaaaagattt gccccggttg atacgaggca
     1021 gtcgatgaag cggttcttta tcaggtaaac aattatttca cgcatgccgg ccgggaccat
     1081 ggcgcccgat aatcccatga atataagcgt gtcctcacgc agcatatttt tccagactgc
     1141 aaatgcctgc gccagggttc ggccctgaaa ggatatattt ttcatgttgt ccagaagccc
     1201 ggcgacattg gatgatttgt caacttgaaa tggtgttgtt ggcttagaga gataatcctt
     1261 caaaatccat actcctgaca aggattacga cttgatgttt acattatgag aatggccgcc
     1321 gccaccaccg tggtccagag gccctccttg tgccccttgg ccgactgggt gatgttacgg
     1381 gtgcggatga ttttgccctg aactttccag atctccttgt tttcgtccca gcttttatca
     1441 aggttgattt ccaggccaag tgtagttgcg agcatgtcga cggccatatc ctcggcatat
     1501 tcacctgcgt gacggccggt ccatccatag gcgtgatgtt ctgaaaggta gccatactgc
     1561 ttgcggtcct ggggtatggc aattccgact gacgcggata tcaggcggtt cggttcattg
     1621 gtagaattct tactgatcac ggtatagaca atttgccctg gacgcagcag tttaaggcct
     1681 tccttcctgg atatgatctt tgcatggggc ggaaatatgg aagatacagc gaccaggttg
     1741 tatttggcta tctcggcatt tctcagagcc agctcaaaag agttcagctt ttccttgtgt
     1801 atgcctaccc ctttggttaa aaacaacttt ttgggagtca tagcatccat ctttaatccc
     1861 tccgattttg tacactgaaa atatgctttt tgcagggaaa attcaacagc taaatgaagt
     1921 aatttgagaa acttatcact tgcaatttgc ccggaattcc ttaaatttcc gtctaaattg
     1981 aaaacgcttg aatgcagaca gaaagctaat ttgaacccgt cgaggagctt atgattaccg
     2041 tgaaagcctt ccgtggactg agaccgaaag aggaggaagc ctcaaagatt gcctctccac
     2101 cgtatgatgt tttgtcttcc caggaggccc gtgagatggc ccgtgagaat ccgatttcat
     2161 tcctgcatat taataaaccc gaaatagacc tgccggagga tacagatgca tattccgatt
     2221 cagtctatca gaaaggccgc gaaaacctga agagattaat ggccgagggt catctgattc
     2281 aggacgataa ggaatcaatc tatatctaca ggcagacctg gagggatcat gtcc
//
LOCUS       DSEB01000355            2140 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1008720, whole genome shotgun sequence.
ACCESSION   DSEB01000355 DSEB01000000
VERSION     DSEB01000355.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1008720"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1615)
                     /locus_tag="ENO07_06320"
     CDS             complement(<1..1615)
                     /locus_tag="ENO07_06320"
                     /inference="COORDINATES: protein motif:HMM:PF03739.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgP/YjgQ family permease"
                     /protein_id="HDS01618.1"
                     /translation="MGILSRYILREHLGPFIFALVITLFVLIIDLVPDIVELIIGKNL
                     DALTVLWVFLLNLAWMLALAVPMACLIATLMAFGRLSSDMEILAIKTSGINMLRIIAP
                     VFIASIILGGGLIWFNNEVLPDANHKARVLMGDIRVMRPTLSIQSNVFLTDIPGYFIL
                     LGDIDHETSRIRDVLIYDQRYSNVRRTITADRGYLEYLEGGQVLSFELEDGEIYESDL
                     TDPTRYKRVLFKKQVFNIRDVSRELRKTSSEYRGDREMSTAQMLAETEDLKGNISGYR
                     DEISKLILSHKDPNNVLRGETTTLREDKIEDIDEIKVSYVMDALNNMRNTMNILKNDF
                     RKINQVQKSINVYLLEVHKKFSIPAACVVFVLIGAPLGMLSRRGGMGTAIGISVGLFI
                     IYWAFLIGGEELSDRGITSPVVAMWAPNILIGAIGLLLLYQLITEKSVLQIINRFRDS
                     RLGARILDWMEKLSKLMKGELEKKGAEPKSRAIWRKHIRPIKILDSYLLGKFLKAVVL
                     SLFIFIIIMHLVHLIEHLDTYLDKRASITD"
     gene            1661..>2140
                     /locus_tag="ENO07_06325"
     CDS             1661..>2140
                     /locus_tag="ENO07_06325"
                     /inference="COORDINATES: protein motif:HMM:PF01336.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01619.1"
                     /translation="MMFKTSSLRLVSGVRQMIKDYILGEQITAFLLLKQIELKISESG
                     SRLALVLGDRTGDIEAVVWNEPEFVKSEIEGAEVVKVQGLVSSYRNKPQITIEKIRKA
                     EAGEFNPDDLKRASSVPPEKLKEEFLAIAESITEPNLRTLLLNFSGDEELFDKWLKRP
                     "
BASE COUNT          549 a          529 c          533 g          529 t
ORIGIN      
        1 catcagtgat acttgcgcgc ttgtcgagat atgtgtcgag atgctcgatc aggtggacga
       61 ggtgcattat gatgatgaaa ataaacagag aaaggactac cgccttgaga aattttccca
      121 gtagatagct gtccaggatt ttgatcggcc tgatatgttt gcgccagatc gccctgcttt
      181 ttggttcggc acctttcttc tccagttcac ctttcatcaa cttggacaat ttttccatcc
      241 agtccaggat tctggctccc aatcttgaat cccggaatct gttgataatc tgcagaaccg
      301 atttttctgt gataagttga tacaaaagca gaaggcctat ggcgcctatg agtatatttg
      361 gcgcccacat ggccacgacc ggcgaagtta tgccccggtc ggatagctct tcgcctccta
      421 tcagaaaagc ccagtagatt atgaacagcc ccaccgatat tccgatcgcg gtacccatac
      481 cgccccgccg cgacagcatc cccagcggcg ccccgatcag cacgaagacc acgcacgcgg
      541 ccgggatcga gaacttctta tgaacctcca gcagatagac attaatcgat ttttgcacct
      601 ggttaatttt gcggaaatca ttcttgagga tattcatggt attgcgcatg ttgttcaggg
      661 catccatgac atagctgact tttatctcat cgatatcctc gattttatct tcccgcaggg
      721 ttgttgtttc gcctcgcaga acattgttgg gatccttgtg gctgaggatc agcttgctga
      781 tctcatccct gtacccgcta atgttgcctt tcaagtcctc ggtttcggcc agcatctgag
      841 cggtggacat ctcacggtca ccgcgatact cggaggatgt tttgcgcaat tcgcggctga
      901 catcgcgaat attgaagacc tgcttcttga aaagcacacg tttgtaccgg gtggggtcgg
      961 tcaggtccga ttcatagatc tcgccatcct cgagttcgaa cgacagaacc tgcccgcctt
     1021 cgagatattc aaggtatccc cggtcggcgg taattgtcct tctgacattg gaataacgct
     1081 ggtcatagat cagtacatcc ctgatcctgg aggtctcgtg atcgatatcc cccaggagga
     1141 tgaagtaacc gggaatatcg gtcagaaaga cattactctg gattgacagg gtcggacgca
     1201 taacccgaat atcgcccatc aggacccggg ccttatggtt ggcatccggc agcacctcat
     1261 tattgaacca gattaatccg ccccccagaa taatgctggc aataaatacg ggggcaatta
     1321 ttctgagcat atttatgccg gaggtcttta tggccagaat ttccatgtcc gagctcaggc
     1381 ggccgaaagc catgagggtg gcgatcaggc aggccatggg aaccgcaagc gcaagcatcc
     1441 aggccagatt cagcaggaaa acccacagca ccgtgagcgc atccagattc ttgccgataa
     1501 tcagttcaac gatatccgga accagatcga taatcagcac aaacagggtg ataaccagcg
     1561 caaatatgaa cgggccgaga tgttcgcgta atatatagcg gctcagaatt cccatgcatg
     1621 ccaaattaag aagattgtta aatcgataca actattttcc ttgatgttta aaacctcgag
     1681 ccttagattg gtttcaggag tacgtcagat gataaaggat tatattctcg gtgaacagat
     1741 caccgcattt cttctgctaa agcaaatcga actcaaaatc tctgagtcgg gcagccggct
     1801 ggccctggtt ctgggcgata gaaccgggga tatcgaagct gtggtctgga atgagccgga
     1861 attcgttaaa tccgaaattg agggagcaga ggtggtcaag gtacagggtc tggtctcaag
     1921 ctaccgcaat aaacctcaaa taacgatcga aaaaatcagg aaagccgagg cgggtgaatt
     1981 taatccggat gatttaaaaa gagcctccag cgttccgcct gagaagctga aggaggagtt
     2041 tctggcaatt gccgaatcca ttaccgagcc caatctcagg acattgctgt tgaatttctc
     2101 cggggatgag gagctgtttg acaagtggct gaaaaggccg
//
LOCUS       DSEB01000356            1982 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1010308, whole genome shotgun sequence.
ACCESSION   DSEB01000356 DSEB01000000
VERSION     DSEB01000356.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1010308"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            8..829
                     /locus_tag="ENO07_06330"
     CDS             8..829
                     /locus_tag="ENO07_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDS01620.1"
                     /translation="MEPVWDKVVDIATVYGAKIIGAILILILGRIAVGIIANAIRRAM
                     VRSEADPTLTKFLVALTKIALFTFVVIAAIATLGVQTASFVAVLGAAGLAIGFALQGS
                     LANFASGVMLIVFRPFKAGDYIEAAGVAGSVEEIHIFNTILKSPDNRKIIIPNSQVTG
                     GSITNYSAKDTRRVDMVFGIGYDDDIKKAKQIMEQIVSEDSRVLKDPAPTIAVSELGD
                     SSVNFVCRPWVKTSDYWAVLFDIHEKVKLAFDEKGISIPFPQTDVHLYQETAAEK"
BASE COUNT          574 a          495 c          478 g          435 t
ORIGIN      
        1 gtttaaaatg gaacctgttt gggacaaggt ggtagatatt gccacggtct atggcgcaaa
       61 aataatcggt gccatactaa tactcatcct cggccgcatt gctgtgggaa ttatcgccaa
      121 tgctatcagg cgagccatgg tgcgctccga ggcagacccc acattgacca aatttctggt
      181 ggccctgacc aaaatcgctc tcttcacctt cgtagttatt gccgcaatcg caacccttgg
      241 tgttcagacc gcgtccttcg tggccgtact gggcgcagcc ggtctggcaa tcggttttgc
      301 cctgcagggt tcgttggcca atttcgcatc gggtgtgatg ttgattgtct tccggccctt
      361 taaagccggc gattatatcg aagccgccgg tgtggcgggt agcgttgaag agatccatat
      421 ctttaacacc atattgaaat ctcctgacaa cagaaaaatc attatcccca acagccaggt
      481 caccggcggg agtattacga actattccgc caaggacacg cgacgggtgg atatggtttt
      541 cggtatcgga tatgatgacg atatcaagaa ggccaagcag attatggagc agattgtcag
      601 cgaggattcg cgagtcttga aggacccggc acccactatt gcagtcagcg agctgggcga
      661 cagctcggtc aatttcgtct gtcggccctg ggtaaagact tctgattact gggcggtatt
      721 atttgatatt catgaaaagg tgaagctggc ctttgacgag aagggcattt ccatcccctt
      781 ccctcagacc gatgttcatc tttatcagga gaccgcggca gaaaaatagc gctgcttcgg
      841 taaatattct taaagctata aacaggatca ggcccgcctg atcctgtttc taaaccattt
      901 ccctgccgtc caaatcaaga gcagatagaa cgggatataa cacatcaacc gcaaatgtat
      961 attttcgctt aatgctccgg cccacggcgc gggatcatga atgctgatat atacattata
     1021 agagagcaga atcgcgcctg tcaggtacca gaaagccggc gacagattgc ggactataag
     1081 cgggtatatc cagaccagat gccagggaaa aagagccgga gtcaaaaccg cataaccgcc
     1141 gaacagcgca aacatcttct gatagacatt ggctcgaatg aataacagca tgagcaacaa
     1201 tgcgcccaat gctccccaga tgacgtatcc cgcagtatcg gttgcaaaga agtgcaataa
     1261 tatatcatag gctgaggcat agaaatgtgg atccatcaga tgtgcgggaa gggctccgaa
     1321 tacatcagga tccgtcccga ttgcataagg caggtacaga aacgccccaa acagcataaa
     1381 tgccgccagg aaaaacagcc gcgaccagct tttgaaatga aaaaacatga agggtagaaa
     1441 aaagaatcca tagaacttaa cgaaagcagc acacgccatc agaaggcccg acaccagcgg
     1501 cttttctctc tccatactta tgaaagccgc aatcaaaaaa ggcagcccca gaatattcag
     1561 atgtgccgat acatagaatt ccaccaatat caatggagag aacagataca acagaagatt
     1621 cgctctttta acgccgataa tctgcagcca cttgagcatc ataaagaatg tcagcacctc
     1681 aaagagcgca aacatcaatt taaatataat aagactgccg cctccgatca tatgagctgc
     1741 cagaaaaaaa tcctgagcca ggggcggata tgcagtgcgc atgtgcggga aatgtatatt
     1801 gggatggatt gtgctgtccc gcagatgagc cacctcatca tcaacggggc tgtatttgta
     1861 tggattgatt ccatccgcgc tgacctttcc atcccagaca taccggtata tatcatcaga
     1921 cagatagaaa tgatcccccg ctcccaccag gacgataagc cgcaccgcca gagcgagcag
     1981 ga
//
LOCUS       DSEB01000357            2430 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1006570, whole genome shotgun sequence.
ACCESSION   DSEB01000357 DSEB01000000
VERSION     DSEB01000357.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1006570"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..203
                     /locus_tag="ENO07_06335"
     CDS             <1..203
                     /locus_tag="ENO07_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004275856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HDS01621.1"
                     /translation="VTPFEPEWEGHATLEISNTTPLPAKIYANEGIAQIIFLEADEEC
                     ETSYKDKKGKYQKQLNITLPRL"
     gene            316..>2430
                     /gene="nifJ"
                     /locus_tag="ENO07_06340"
     CDS             316..>2430
                     /gene="nifJ"
                     /locus_tag="ENO07_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDS01622.1"
                     /translation="MSSDKKRRMVTIDGNTAAAYVAHATNEVIAIYPITPSSNMGELA
                     DAKSAAGERNIWGTIPTVVEMQSEGGASGAVHGAITTGALTTTFTASQGLLLMIPNMF
                     KIAGELTPVVFHVSARAVATHALSIFGDHSDVMATRTTGFGLMASGSVQEVMDLALIT
                     QAASLRSLVPFVHFFDGFRTSHEVAKVQEIAFDDMRAMLPYEIVKRHRDRAMNPEHPV
                     LKGTSQNPDVFFTSRETVNKYYDVAPEIVQKEMDKFAKIVGRKYKLFDYFGDPEAERV
                     VFIMGTGAETMHETVDYLNQQGEKVGLIKVRLYRPFSVKAMAEALPATVKKIAVLDRT
                     KEPGSVGEPLYTDVQTGVVEAMHKGWTKFKEKPVIVGGRYGLGSAEFNPAMARSVLDN
                     LKQDDPKDHFTAGIKDDVTMTSLDFDRDFDIEGEDVFRGMFYGLGADGTVGANKNSIK
                     IIGENTDNYAQGYFVYDSKKAGAITISHVRFGPREIQRPYLISKANFIACHNFTFLEK
                     IDMLENIIEGGTFLLNSMYGPDEVWNKIPKEVQKQIIDKNLEFYVIDGIGLAKELGLG
                     ARINMIMQTAFFAITEILPKDQAVKAIKDAIKKTYGRKGEKVVQMNYDAVDAALKNYH
                     KVDYPKEVTSEISMPPTVPENAPDFVKNVTAEIIKGKGHELPVSAFPFDGAYMTGTTQ
                     YEKRNIAVDIPVWEPDICIQCNI"
BASE COUNT          667 a          588 c          644 g          531 t
ORIGIN      
        1 atgttacacc tttcgagccg gaatgggagg gtcatgccac tctggagatc tcgaacacca
       61 cccccctccc cgcaaagata tatgccaacg agggaatcgc gcagataatt ttcctcgaag
      121 cagacgagga atgcgagacg tcatataaag ataaaaaggg aaaataccag aaacagttaa
      181 atattaccct tccgcgactt taatctcacg tatatagttg acaagccaga tgaagggcat
      241 taattaaaga taaaataaac agaaaagagc agatatatgg gtacagaaac aaaagaaaaa
      301 gaattggagc aagcgatgtc ttccgacaag aagcgcagga tggtcacgat agacggcaac
      361 actgccgccg cctatgtggc gcatgcgaca aacgaggtaa ttgctatata tccgattact
      421 ccctcttcga atatgggtga gcttgccgat gccaaatctg ctgcaggcga aaggaatatc
      481 tggggaacga taccgactgt ggttgaaatg cagtccgagg gcggagcctc gggcgccgtg
      541 cacggcgcta tcacgaccgg cgcgcttaca accaccttta ccgcctccca gggccttctt
      601 ctgatgatac cgaatatgtt caagatagcc ggcgaactga caccggttgt gtttcatgtt
      661 tcggcacgtg cagtagcgac acacgctctc tcaatatttg gagatcattc cgatgttatg
      721 gccacccgta ccaccgggtt tggcctgatg gcgtccggct cggtgcaaga agtcatggac
      781 ctggcgctca tcacccaggc cgcgtcactc aggagcctgg taccgttcgt gcatttcttc
      841 gacggattcc gaacctctca tgaggtggcc aaagtgcagg aaatcgcatt cgacgacatg
      901 cgcgccatgc ttccctatga gattgtaaaa cggcacaggg acagggccat gaatccggaa
      961 catccggtgc tgaagggcac ctcccagaat cccgatgtat tcttcacctc acgtgaaacc
     1021 gtaaacaaat attatgatgt cgcccctgaa atcgtccaga aagagatgga caaattcgcg
     1081 aagatagtgg gacggaagta caagctgttc gactacttcg gagatccgga agcggagcga
     1141 gtggtcttta taatgggtac cggcgcggaa actatgcatg agacagttga ttatctcaat
     1201 cagcagggcg agaaagtcgg cctgatcaag gtcaggctct atcggccatt ctcggtcaag
     1261 gccatggctg aggccctgcc cgcgacagta aagaaaatag cggtactgga cagaaccaag
     1321 gagcccggct ctgtaggtga gccgctttat accgatgtcc agaccggcgt ggtggaagct
     1381 atgcacaagg gctggactaa attcaaagag aagccggtca ttgtgggcgg ccgctacggt
     1441 ctgggttcgg ctgagtttaa tccggctatg gccaggtccg tgctggataa cctgaaacag
     1501 gatgatccca aggatcattt cacagccggt attaaagatg acgtcaccat gaccagtctc
     1561 gattttgatc gcgacttcga catcgagggt gaagatgtct ttcgtggtat gttctatggc
     1621 ctgggcgccg atggtacggt gggcgcgaac aagaactcga ttaaaattat tggcgagaac
     1681 actgataact acgcccaggg ctattttgta tatgactcca aaaaggccgg cgcgatcact
     1741 atctcgcatg tgcgtttcgg accgcgtgaa atccagcgtc cgtacctgat ttccaaggcc
     1801 aacttcatcg cctgtcacaa ttttacattt ctcgagaaaa tcgatatgct cgagaatatt
     1861 atcgagggtg ggacatttct gttaaacagc atgtacggcc cggacgaggt ctggaacaag
     1921 attcccaaag aagtgcagaa acagataata gacaagaatc tggaattcta tgttatcgat
     1981 ggtatcggcc tggccaagga gctcggactg ggtgcgagaa tcaatatgat catgcagaca
     2041 gctttctttg caatcaccga gattctcccc aaagatcagg cggtcaaggc catcaaagac
     2101 gctatcaaaa agacctacgg ccgcaagggc gagaaggtcg tacagatgaa ttatgatgcg
     2161 gtcgatgcgg cgctcaagaa ttatcataag gtcgattatc cgaaagaggt caccagcgaa
     2221 atcagcatgc ccccgacggt acctgaaaat gctccggatt ttgttaagaa tgttactgct
     2281 gagattataa agggcaaggg ccatgagctt ccggtttcag ccttcccgtt tgacggcgcc
     2341 tatatgaccg gaaccacgca gtacgagaag cgtaatattg cggtcgatat cccggtctgg
     2401 gagccggata tctgcataca gtgtaatatc
//
LOCUS       DSEB01000358            2897 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004439, whole genome shotgun sequence.
ACCESSION   DSEB01000358 DSEB01000000
VERSION     DSEB01000358.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004439"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            53..400
                     /locus_tag="ENO07_06345"
     CDS             53..400
                     /locus_tag="ENO07_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01623.1"
                     /translation="MSHSDKNAVILLSHKELGVLSTLELKPDILIIPEPAYISKGFIE
                     SIRHFKMETLIFCSYRSLYWSPPGYEKIINHISGKKIDFYNVRECGAVRIELKPSGIS
                     INPVLDCNYRQTD"
     gene            547..1737
                     /locus_tag="ENO07_06350"
     CDS             547..1737
                     /locus_tag="ENO07_06350"
                     /inference="COORDINATES: protein motif:HMM:PF14332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HDS01624.1"
                     /translation="MSFTGNLKTVAFPDILQLLSSGKKTGILVITKGKIQKEICFKDG
                     NIIGAYSKNAEEDFLGNLLLKQGRISRIDLERALHLHKSTGKKLGMVLVDMELIDHNE
                     IGKILKLQVEEIVFNLFSWHEGEFVFQEGKLPPEKEFFVELNTMNMLMEGTRRIDEWV
                     EITKGLPKDEELLRVVLTPETKTGEVTISLDQFQVLSLINGERTVPDIVSESPVGEFI
                     TKRGLHQLLQAGLIEVAGIKKESKTEQFGDDDSLWWLLLRLYSSCFAAIRRNLDQKLG
                     PDNQKVREHLSRYSTGVWGFFSNSTSSDFRTTFNKFKTTVQKIPKEAQVYRMLSGLNQ
                     ILTDQMSLVSSLLGDNVRRQVASEIRKEIAMPLAEKREINKKFGIERDLARALTEFRQ
                     VKNA"
     gene            1808..2113
                     /locus_tag="ENO07_06355"
     CDS             1808..2113
                     /locus_tag="ENO07_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01625.1"
                     /translation="MADAASGGSDVGVLFNGLILLVVLVCFFVAMKIFSLLRGGELAS
                     GWQMLAIAFIILCLGQLIDLMATLEIGGLNQAIVATVRLAGVFLLLLGVVRIKKVLS"
     gene            2151..>2897
                     /locus_tag="ENO07_06360"
     CDS             2151..>2897
                     /locus_tag="ENO07_06360"
                     /inference="COORDINATES: protein motif:HMM:PF13428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDS01626.1"
                     /translation="MLNQAALDERISKCRGILDENPNSQIFAALADAYRKNGELDKAF
                     RVCRQGLRIHPEYGAGHLIMAKINFDRKMYDWAESELKEAINYDGRTRATDMLNAKIK
                     TKQGKLDEAKVLVNELLSTDPQNEQLNELLGRIERGRLEQKKKEMESRRLFESRALAE
                     DKDLLQKENMQPTLNSTEALNKIASFPGMNACFFTNHNGMMVEADLPEYFDQDSYAAL
                     STEIYRFANENVGSVRFGRVKQVLIELEGEK"
BASE COUNT          813 a          589 c          770 g          725 t
ORIGIN      
        1 ggaaatatcg ctattaatgt tggaggagga aaacaggctt atcggagcgg ttgtgagcca
       61 ttccgataaa aatgcagtga tcttattgtc gcataaagaa ctgggtgtgc tgagcactct
      121 tgagctcaag ccggatattt tgattatacc ggagccggcc tatataagta agggtttcat
      181 agagtcaatc agacatttta agatggaaac attaatattt tgcagttaca ggtcgcttta
      241 ttggtctccc ccggggtatg agaagatcat aaatcatatt tccggaaaaa aaattgattt
      301 ttacaatgta cgcgagtgcg gggctgtcag aatcgagctg aagccttccg ggatcagcat
      361 aaatccagtt ctcgactgta attataggca gacagattag aactacatga gtattggaca
      421 gctccaataa aaatcagcgc atctttttgt gttaaatcca ctttattcgt aaagttaact
      481 caggtatatc ccgataaaat tagagaaaga gtaggtcgtc tatttgaaaa aaacaggatt
      541 aatcctatga gctttaccgg caatcttaag acggttgcgt ttccagatat tttacagttg
      601 ttgtcgagcg gaaagaagac tggaatcctg gtcatcacca agggaaaaat ccagaaagag
      661 atctgtttta aagacggcaa cataatcggg gcatactcca aaaatgccga ggaggacttc
      721 cttggcaatc tgcttttgaa gcaggggcgt atatccagga tagatctgga aagagcgctg
      781 catctccata aaagcacagg caagaaactg ggtatggttc ttgtcgatat ggagcttatc
      841 gaccataatg aaatcggtaa aattttgaag ctgcaggttg aagagattgt ctttaacctt
      901 ttcagctggc acgaaggcga attcgtcttc caggaaggca agctgccgcc cgaaaaggaa
      961 ttcttcgtcg agctgaatac aatgaatatg ctgatggagg ggacccgtcg aattgacgaa
     1021 tgggtggaaa tcacaaaagg gcttcccaaa gatgaggaac ttctcagggt ggtgctgact
     1081 cccgagacaa aaacggggga ggtaaccatc tccctggatc agttccaggt tttatcgctg
     1141 ataaatggtg agcggacggt tcctgatata gtctcggaat cgccggtggg agagtttatc
     1201 accaaacgcg gcttacatca gctcctgcag gccggactga tcgaggtcgc cggtatcaag
     1261 aaagagagca aaacggagca gttcggagat gatgacagcc tctggtggct tcttctgaga
     1321 ctttattcga gttgttttgc ggcgatcagg agaaacctgg atcagaaact tggccccgac
     1381 aaccagaagg tccgggaaca tctgtcacgc tacagcactg gagtctgggg ctttttctct
     1441 aactcgactt catccgattt ccgcacgact ttcaataaat tcaagaccac ggttcagaag
     1501 attcccaagg aggcccaggt ctatcgtatg ttgagcggtc tgaaccagat cctgaccgac
     1561 cagatgagcc tggtttcctc tttactggga gacaatgtgc ggcgtcaggt ggcctcagaa
     1621 ataaggaaag agatagccat gcccctggcc gagaagcgtg aaatcaataa gaaatttgga
     1681 atagaaagag atttagccag agccctgacc gaatttcgtc aggttaagaa tgcgtgaggt
     1741 ggagatgtat ataagcaggt tattaatatt tgccctggca gcactattat ttttgccgtc
     1801 cgctctgatg gcagacgcgg cttccggcgg ttccgatgtg ggcgtgctat tcaacgggct
     1861 gattttactg gtagtgttgg tctgcttttt tgtagcgatg aaaatatttt cgcttctgcg
     1921 cgggggcgaa cttgcctccg gatggcagat gctggcaatc gcttttatta ttttgtgcct
     1981 gggccagttg attgacctga tggctaccct tgagataggt ggtctcaatc aggcgatagt
     2041 tgcgaccgta aggctggcgg gagtattcct gctgcttttg ggagttgtaa gaattaagaa
     2101 ggtcttaagt tgaggcacag aagcgatgct tccttccggg aggagatgta atgcttaacc
     2161 aagcagccct cgatgagcgc attagcaaat gcagaggaat tctggatgag aatccaaatt
     2221 ctcagatatt cgctgcattg gccgacgctt atcgaaaaaa tggagagttg gataaggctt
     2281 tcagagtttg ccggcaaggc cttcgaattc atcccgagta cggcgcggga cacctgataa
     2341 tggccaagat aaattttgat cgcaagatgt atgattgggc ggaaagtgaa ttaaaggaag
     2401 ccattaacta tgatggccgc acacgcgcaa ccgacatgct caatgcgaaa attaagacca
     2461 agcaaggtaa actggatgaa gccaaggtcc ttgttaatga gcttctttct acagatccgc
     2521 agaacgagca gttgaatgaa ctgctgggta ggatcgagag gggccgactg gagcagaaga
     2581 aaaaggaaat ggagtcgcgc aggctgttcg agtctcgcgc tttggcggag gataaggatt
     2641 tgctgcagaa agaaaatatg cagcccaccc tgaattccac cgaagccctg aacaaaatcg
     2701 ccagcttccc cggcatgaac gcttgtttct tcacgaatca caacggcatg atggtcgagg
     2761 ccgatctgcc cgaatatttt gatcaggatt cctatgctgc tctgtcaacc gaaatatacc
     2821 gctttgctaa tgaaaatgta gggtcggtaa gattcggcag ggtgaaacag gttcttatcg
     2881 agctggaagg tgaaaaa
//
LOCUS       DSEB01000359            8670 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000409, whole genome shotgun sequence.
ACCESSION   DSEB01000359 DSEB01000000
VERSION     DSEB01000359.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8670)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8670)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8670
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000409"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1683)
                     /locus_tag="ENO07_06365"
     CDS             complement(<1..1683)
                     /locus_tag="ENO07_06365"
                     /inference="COORDINATES: protein motif:HMM:PF13432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDS01627.1"
                     /translation="MLKKNIFFLVIISLFVFSITVNALPPAETRTVERAQRYAELGRV
                     EEAIKMIEPLLDRFPADLKLLTSAHRIYLEAKDYEKALKILDMQKKVSLNGGRLYLDY
                     ADIFLKMGKMESADSVFQVFLKERGGGSQAYREISQALLNNGYYAEATQVYLDGRERL
                     MDSAQFALELGNLYQRQRQYANAAREYFRFMVYDTAMHRTGEAQIRFLINNADEIEEL
                     KQAFRDIIAGYPDNYQSYQFYAELLVREGQYDSAFENYKIVDSLHFRNGQHLYGFAEA
                     ALNDHEFEMAARTCRYLLEKYPEQGYRERAQLTLAAALIQLGQPDSAVLVYHQVIESS
                     PSPKLKFDGEYLLGILFLQELYQSDSARYYFNRILNSPQMQPWAEMVKIKIAESYIID
                     NELDTADSIFLSINTNGLKNDMKEELLFKRAQIKFFKQEFTDARGLYNHLTGLYPRSM
                     YVNDCLRKILIIDENQGTGVIDLDMYAAAERLLWQNEADSALTKLVDLSGRGNSNLSA
                     MATFLAGEILYEKEEYQQALEYFNRVLESFEESFYTAESQKYLGDLYYYHLND"
     gene            complement(1683..5081)
                     /locus_tag="ENO07_06370"
     CDS             complement(1683..5081)
                     /locus_tag="ENO07_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01628.1"
                     /translation="MPDDNRIALITSRLKRLGGYILGKRILYGLLLLLSLFLAASFLA
                     ALSHAVFYLPAWYRTAFVAAVMAAGVGLFLWFIAWPIIRRPSNEELALLVEKRYPDLK
                     NRMIAALQLERKLLNNKENYSPALIEQCIRQAEELSDQIEFKKSYQSSKVSSGFKYFG
                     ATLIMTFAFWLLSPGIFNSSMEVFSNPLKEIPRVLTYDLQVSPQNADILKFDDLEIDA
                     FVFGSKLPNKARIHWKLADDWRSDEIEKQDRIENVPETQSGLLAGAPDTSLFKYDFKE
                     IRHDFQYYVEAGDRISEIYNVRVVDKPRINNIKLTYFYPKYTGLAPVVLDENDGTIQA
                     LKGTRVKIEGELNKPVVEGRIVYEDGESDELQIDDNRIGKTLKIMGNGSYHLEVTDNI
                     GHTNPDPIEYKIYMTEDNYPSVEVVRPGNNIDLDDYLAFDLAATLSDDYGFSRLVLHY
                     TVHLSPMEASSDSIEFKFDKSKTDQLIEFYWDLSNKGLYPGSYVDYYFEVWDNDNISG
                     PKSAVSRTYSARHPTIEEMFVDIEEAREDMISEMVEAFRQEMQLQERAKELREDLQFQ
                     DETEWETQKDIEKMQQEQQEVMDNLEKMAEQFDKLNQEAKKNDLLTLEMIQKLNELQK
                     LFDEVATEEMKEAMKQLQEALENMDKSELEQAMKDFEMSTEEMIQNIERSIAQLKRFQ
                     IEQKMQAMIAMAEKILENQNAVNEKAEQSEESELPNLKSREDQNASDTKQLKEEASRL
                     REMLAENQLEMEPNAGEFCQSVENTDADMDMNEMSESLQNQQKPEATNSGQSASAKIE
                     DMLAQMKNAQAKFNNQMSAEMAQKMREAIDDLLYLSDEQEALYNEILPLNPHSQVLPE
                     YAQKQQDLRSEAERLRQELQEVAKQSVFIQNALDEYMKGVCNSMESSISSLTEKNGRS
                     AASHQSDGIYSLNQSAQTLIESLNSQSQCNSSCSNNQSMFQKMNKLSKGQQKVNKQTQ
                     SMCDNPGQKPGKPSEDAMRRLAAQQQGLRQGISEMIDEFGDRKDVAGRLDKMAEEMKK
                     VIEALESGDVGSPTLERQKRVYSRMLDFQLSLERRDYSEKRRADTGADVVRRSPAELE
                     LEKRMQESAYRARLEKFLDEAYPPEYEALIKDYYKALINNDRDK"
     gene            complement(5092..5781)
                     /locus_tag="ENO07_06375"
     CDS             complement(5092..5781)
                     /locus_tag="ENO07_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HDS01629.1"
                     /translation="MTKAFRFCFCTILPALILFTVPISAQTADDNPSAVQIARLKYFG
                     GGDWYWGPSALPNLIDFVNRNCPIEIASEEVRLTLTDEKLFHYPFLFLTGHGPIRFSE
                     EEAGRLREYLSAGGFLFANDSYGMKESFFESMKEVFPENDPVELPFSYGIFNCYYNFS
                     NGLPKIHKHEEKPPQAFGWFLDDRLVALFAYESDIGDGWEDPSVHNDPPEKRQAALRM
                     GANILTWVLLN"
     gene            complement(6023..6235)
                     /locus_tag="ENO07_06380"
     CDS             complement(6023..6235)
                     /locus_tag="ENO07_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01630.1"
                     /translation="MSMKPIDISGGESVYCFNEAQMKKALELANNNPESSERIQSHLK
                     RIESDLNRNEQAALAFVIVDSLIKSH"
     gene            complement(6259..6573)
                     /locus_tag="ENO07_06385"
     CDS             complement(6259..6573)
                     /locus_tag="ENO07_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HDS01631.1"
                     /translation="MEISRKARSELLSLKGDPDDFLRIRVITGGCAGLTYRPGFDCEM
                     GDDDVVLYENSGLKVVADMKSALYTHGLEIDYSDDLINSGFRFANRRARKSCGCGQSF
                     EA"
     gene            complement(6725..7537)
                     /locus_tag="ENO07_06390"
     CDS             complement(6725..7537)
                     /locus_tag="ENO07_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDS01632.1"
                     /translation="MNIIHFFEGLHPIVQALVGTLFTYFMTAAGSATVFFKGEFSRKT
                     LDGMLGFAAGVMIAASYWSLLAPAIAMSEGKSVPAYVPALVGFILGAICLRLIDMVLP
                     HLHAMYNDEYEGISTTWRRTTLLILAITLHNFPEGLAVGVAFGAAAAGLEEATIAGAV
                     ALALGIGIQNFPEGAAVSLPLRREQRSRLKSFFYGQLSGVVEPVAGVMGAALVLIAKP
                     LLPYALAFAAGAMIFVVVEELVPEASREHSHAATAGVILGFAVMMTLDVALG"
     gene            complement(7600..8622)
                     /gene="cydB"
                     /locus_tag="ENO07_06395"
     CDS             complement(7600..8622)
                     /gene="cydB"
                     /locus_tag="ENO07_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDS01633.1"
                     /translation="MSLDLNTIWFILVGVLFTGYAILDGFDLGTGALHLFTSTDKERR
                     IMLNAIGPVWDGNEVWLVTGGGALFAAFPEVYATAFSGFYMAIMLLLLGLIFRAVAID
                     FRSKRESTTWRQFWDISFSFSSILSSLLIGIALGNIAWGIPLDGDFEYAGRFIRLLHP
                     YALLVGITTVALFMMHGAIYLLMKTEGELQSKIRAWVNPTIIFFIICYILTTMATLLY
                     VPHMSDNLKAFPPLMVIPLLNALAIANIPREIHHGREFRAFLSSCAAMAALLALFGIG
                     MYPDLIMSNPLAANSLTIYNAASSAKTLKIMLIIAILGMPLVIAYTSSIYWIFRGKVK
                     LDSSSY"
BASE COUNT         2122 a         2276 c         1984 g         2288 t
ORIGIN      
        1 atcattgaga tggtaataat aaagatcccc cagatatttc tgcgactcag cagtgtagaa
       61 gctctcctcg aaactctcga gcacacggtt gaaatattca agtgcctgct ggtattcctc
      121 tttttcgtat aaaatctcgc ctgccaggaa ggtcgccatg gccgagaggt tggaattgcc
      181 ccggccggac aggtcgacca gctttgtcag cgccgaatcc gcctcgttct gccagagcag
      241 acgttctgcg gcggcataca tatccaggtc gataacacct gtaccctggt tctcatcgat
      301 aataaggatc ttcctgagac agtcattgac gtacatgctg cgcggatata ggccggtcaa
      361 gtggttatat agtcccctgg catcggtgaa ttcctgtttg aagaacttaa tctgagccct
      421 tttaaaaagc agttcctcct tcatatcatt cttcaggcca tttgtattaa tggatagaaa
      481 aatgctgtcg gcggtatcga gttcattatc gattatatat gattcagcta tcttaatctt
      541 gaccatctcc gcccagggct gcatctgggg actgttcaaa attctgttga aataatagcg
      601 tgccgagtcg ctttgataga gttcctgcaa aaacagaatc cccagcaaat attcaccgtc
      661 gaacttcagt tttggagatg ggcttgattc aattacctgg tgatagacaa gcacggccga
      721 atcaggctgg ccgagctgaa tcaaagccgc cgccagggta agctgagctc tttccctgta
      781 gccctgttcg gggtactttt ccagcaggta gcggcaggtc ctggcagcca tctcaaattc
      841 gtggtcgttt agagctgctt ccgcaaaccc atacaggtgc tgaccattcc tgaagtgcag
      901 ggaatcgaca attttataat tttcaaatgc gctgtcgtac tgtccttccc ggaccaggag
      961 ttctgcataa aattgatagg actgatagtt gtcgggatag ccggcaatta tatccctgaa
     1021 tgcctgcttc aattcctcga tctcatcggc attgttaatc aggaacctga tctgtgcctc
     1081 acccgtcctg tgcatggcgg tatcatagac catgaacctg aaatactctc gggcggcgtt
     1141 ggcatactgt ctctggcgct gatagaggtt tccaagttcg agggcaaact gggccgaatc
     1201 cattaacctc tcccggccgt cgagataaac ctgggtcgcc tcggcatagt atccattgtt
     1261 caataaggcc tgcgagattt ctctgtaagc ctgggacccg cctccgcgct ccttcaggaa
     1321 aacttgaaaa accgaatcgg cagattccat cttgcccatc ttaagaaaga tgtcggcata
     1381 atccaggtac agccgtccgc cattgagact gaccttcttc tgcatatcca gaatcttaag
     1441 ggccttctca tagtccttgg cttccagata aatgcggtgt gccgaggtca gcagtttaag
     1501 gtcggccgga aagcgatcca gaagcggttc tatcatcttt atggcttctt cgacacgccc
     1561 gagttcagca tatctctggg cccgctctac agttctggtc tcggccggtg gcagagcatt
     1621 aacggtgatc gaaaaaacaa atagagatat aataacaagg aaaaatatat tctttttcag
     1681 catcacttat cccggtcgtt gtttataaga gctttgtaat aatccttgat caaggcttcg
     1741 tattccggcg gataggcttc atcaaggaat ttttccagcc tggcccgata ggcggactcc
     1801 tgcatgcgtt tctccagttc cagttccgcc ggagacctgc ggacaacatc cgctcctgta
     1861 tccgccctgc gtttttcgga ataatcccgt ctttcaagtg aaagctggaa atcgagcatg
     1921 cgcgaataaa cgcgtttctg tctttccagt gtaggactgc ccacatcacc ggattcaaga
     1981 gcctcgataa ctttcttcat ctcctcggcc atcttatcca gacgaccggc gacatccttg
     2041 cggtcgccga actcgtcgat catctcgctg atcccctgcc tcaggccctg ctgctgagct
     2101 gccaggcggc gcatcgcgtc ctcgctgggc ttgcccggtt tttgtccggg attgtcgcac
     2161 atgctctggg tctgtttatt taccttctgc tgtcccttgg acaacttgtt cattttctgg
     2221 aacatggatt gattattgct gcagctcgaa ttgcactgtg actgactgtt taaggactcg
     2281 atcaatgtct gcgctgactg attgagagaa tagattccat cggactggtg actggcggca
     2341 gagcgcccgt tcttctcggt cagtgatgat atcgaactct ccatcgaatt gcagacaccc
     2401 ttcatatact cgtccagggc gttctggatg aagaccgatt gcttggcaac ctcctgaagt
     2461 tcctggcgca gacgctccgc ctcacttctc agatcctgct gcttctgggc atactccggc
     2521 agtacctggg agtggggatt aagcggaagg atctcgttgt atagtgcttc ctgctcatcc
     2581 gaaaggtaga ggaggtcgtc gatcgcctcg cgcatcttct gggccatctc ggccgacatc
     2641 tgattgttga atttcgcctg ggcattcttc atctgcgcga gcatatcctc gatcttggct
     2701 gaagcgctct ggccggaatt agtggcctcc ggcttttgct ggttctgcag gctctcagac
     2761 atctcgttca tatccatatc ggcgtcagtg ttttccactg actggcagaa ttcccctgca
     2821 ttgggttcca tttcaagctg gttttccgcc agcatttcac gcagcctgga tgcttcctcc
     2881 ttgagctgtt tggtatcgga ggcgttctgg tcttccctgc tcttgagatt aggcagctcc
     2941 gattcctcag actgctcggc tttttcgttt acggcattct gattctcgag aattttttca
     3001 gccatggcaa tcatggcctg catcttctgc tcgatctgga atcgcttgag ctgagctatg
     3061 gagcgttcga tattctggat catctcctcg gtggacatct cgaaatcctt catggcctgc
     3121 tcgagctcac tcttatccat attctccaat gcctcctgaa gctgtttcat cgcctctttc
     3181 atttcctcgg tagcgacctc atcgaaaagc ttctggagct cgtttaactt ctgaatcatt
     3241 tccagcgtga gaaggtcgtt tttcttggct tcctggttga gcttgtcgaa ctgctcggcc
     3301 attttttcca ggttatccat cacctcctgc tgttcctgct gcatcttctc gatatctttt
     3361 tgtgtttccc attcggtctc atcctggaac tggaggtcct cacggagttc cttggcgcgc
     3421 tcctgcaact gcatctcctg cctgaaagcc tcgaccattt cggagatcat atcctcacgc
     3481 gcctcctcga tatcaacgaa catctcctcg atggtaggat gacgcgcact ataggttctg
     3541 gaaactgccg acttggggcc ggaaatatta tcattgtccc agacttcgaa gtaataatcg
     3601 acatagctgc cgggatagag acctttattg gagagatccc agtagaattc tatcaactga
     3661 tcggtttttg atttgtcaaa tttgaactcg atcgaatcac tgcttgcctc catcggcgac
     3721 agatgtacag tgtaatgcag tacgaggcgc gagaaaccat agtcgtcgga gagcgtggcc
     3781 gccagatcga aggccagata atcatcgaga tcgatattat ttccaggcct gaccacctcg
     3841 accgatgggt aattgtcttc ggtcatataa attttgtact cgatcgggtc cggattggta
     3901 tgacctatat tgtcggtaac ttcaaggtgg tacgagccgt ttcccatgat ctttaatgtc
     3961 tttccaatcc tgttatcgtc gatctgcagt tcatcactct caccatcttc atagacaatt
     4021 ctgccctcca cgacaggctt gttcaattct ccctctattt tgaccctggt cccctttaaa
     4081 gcctggattg tgccgtcatt ttcgtccagc accaccggcg caaggccggt atatttggga
     4141 tagaaatatg tgagcttgat attattgata cggggcttat ccacaacgcg cacgttgtat
     4201 atctctgaga tcctgtcgcc cgcctcgaca tagtactgga aatcatgcct gatctctttg
     4261 aaatcatact taaagagcga ggtgtcggga gcccccgcca gcaagccgga ctgagtctca
     4321 ggaacattct cgattctatc ctgtttctca atctcgtctg aacgccagtc atctgccagt
     4381 ttccagtgaa ttctggcttt attggggagc ttactcccga atacaaaggc atcgatctcc
     4441 aggtcatcaa atttgagaat atcggcattt tggggagata cctgcagatc atatgtcagt
     4501 actctgggaa tctcttttaa cggattggag aaaacctcca ttgaagaatt gaatataccc
     4561 ggagaaagga gccagaacgc aaatgtcatt atcagggtcg caccgaaata tttgaagccg
     4621 ctggacactt tgctggattg atagcttttt ttgaattcta tctggtctga caattcctca
     4681 gcctgccgga tacactgttc gatcagagcg ggagaataat tctctttatt gttcaagagc
     4741 tttcgttcca actgcaatgc tgcaatcatc cgatttttca ggtcgggata ccgtttctcg
     4801 accagaagag ccaactcttc atttgagggt cttcggatta tgggccaggc aataaaccac
     4861 aggaaaaggc ccacacccgc ggccattacc gccgccacaa atgcggtacg gtaccaggcg
     4921 ggaagataga acacggcatg actcaatgcg gccagaaaac tcgctgccag aaaaagcgac
     4981 agcaacagta aaaggccgta caaaattctt tttccaagga tgtagccccc cagcctttta
     5041 aggcgcgatg ttataagcgc aattctgttg tcgtcgggca tacaatcctc actaatttaa
     5101 aagtacccag gttaatatat tggctcccat gcgcaaagcg gcctggcgct tctcgggggg
     5161 atcattatga acagatggat cttcccatcc gtccccgata tccgattcat aggcgaaaag
     5221 tgccaccagg cggtcgtcta aaaaccatcc gaatgcctgc ggcggctttt cttcatgctt
     5281 atgtattttc ggcagaccgt tggagaaatt gtaataacaa ttgaatatac cataactgaa
     5341 aggaagctcg accggatcat tttccgggaa gacctctttc atcgactcga agaaggactc
     5401 tttcataccg taagaatcgt tggcaaacag gaatcctccg gcggaaaggt attcccttaa
     5461 tctaccggcc tcttcctcgg aaaagcgaat cgggccatgt ccggtcaaaa acaggaaagg
     5521 ataatgaaag agcttttcat cagtcagggt caaccttacc tcctccgaag ctatctcaat
     5581 cggacagtta cggttaacaa aatctatcag gttcggcaga gctgatggtc cccagtacca
     5641 gtcccctccg ccgaaatatt ttaagcgggc aatctgcacc gcgctgggat tatcatcagc
     5701 ggtttgagca gagataggga ctgtaaatag tatcaaagcc ggaagtatcg tgcaaaaaca
     5761 gaatctgaat gcctttgtca tagtatatta aacagccttc cgaagtaaaa gtttggaatc
     5821 agccctgaaa aacctaaaag ataagtaatc ttgtgctatt tctatctatt gtacgcaaaa
     5881 atacaacttt gtcaagtcac taaattcttc aagattatct actgcataaa agcaattatt
     5941 ccccatcttt tttacattcc cgctaaagaa atgaccgcag tcaccaggag agttgacctg
     6001 ttttcatttc agaccgcggc cattaatggg attttatcaa actatccaca atcacaaacg
     6061 ccagggctgc ttgttcattg cggttcaaat ccgattcaat ccttttcaag tgactctgaa
     6121 tcctttcaga actctccgga ttgttattcg ccagttccag cgctttcttc atctgagctt
     6181 cattgaagca atagaccgat tccccgccgg aaatgtcgat tggcttcata ctcatataaa
     6241 caactcaggt ttataatgtt aagcctcaaa tgactgcccg catccgcagg atttccgggc
     6301 tcttctgttg gcgaaacgaa aaccggaatt gataagatca tccgaataat ctatctcaag
     6361 gccatgggtg taaagcgcac ttttcatatc cgcaaccacc ttcaggccac tattttcata
     6421 caggaccaca tcatcatccc ccatttcgca gtcgaaaccg ggacgatagg tcaaaccggc
     6481 acatccgcct gtaatcaccc ttatccgcag aaagtcatcc ggatcgccct tcaggctgag
     6541 caattcgctc cgagccttcc tgctaatctc tatattcacc ggtacaactc ctttcggttc
     6601 tatcctattt cacgggtata tacggcaagt atacaggatt atatttatcc tgtatatatt
     6661 aataccgatt tcccctgaaa gttccggaaa actggcgctg attgctaatt caacggggtg
     6721 aaactcagcc cagggcgaca tcgagggtca tcatcaccgc aaaacccagg attacaccag
     6781 ccgtagcggc atgggagtgt tccctgctgg cctccggcac cagctcctca accaccacga
     6841 agatcatagc gccggctgca aacgccagag catacggcag aagcggcttt gcgatcaaga
     6901 ccagggcagc gcccattacg cccgctacag gctcgaccac acctgacaac tgtccataga
     6961 aaaagctctt caggcgggat ctctgttctc ttctcaatgg cagggagact gccgcgccct
     7021 cagggaaatt ctggatgcct attcctagag ccagtgccac cgctccggca attgtggctt
     7081 cctcgagtcc ggcggcagca gctccaaagg ctacccccac cgcaagccct tcggggaaat
     7141 tatggagcgt gatagcaagg atgagcagtg ttgttctccg ccaggttgtg ctaattcctt
     7201 catattcatc attatacatg gcgtgaagat gcggtagcac catgtcgatc aggcgtaggc
     7261 atatggcgcc caaaataaag ccgacaagtg caggcacata tgcgggtacg gatttacctt
     7321 ccgacattgc aattgcagga gccagcagtg accagtagct ggcggctatc atcacgcccg
     7381 cggcaaatcc aagcatgcca tccagagtct tgcgactgaa ctctccttta aaaaagaccg
     7441 tagcggaccc ggcggcggtc ataaaataag tgaataatgt tccgaccagc gcctggacaa
     7501 ttggatgtaa gccttcgaaa aaatgtatta tattcattat atatttacgt ttttcttcta
     7561 ctccagcttc aactcaatct agagacctcc agcattgact cagtaactcg atgaatccag
     7621 ttttactttg ccccggaata tccagtatat gctgctggta tatgcgatca ccagaggcat
     7681 acccagtatc gcgataatca gcatgatctt caaagttttg gctgaagagg ccgcattata
     7741 tatggtcagg ctgttggctg ccaagggatt agacataata agatctggat acatgccgat
     7801 tccaaagagc gccagaaggg cagccatggc cgcacaggag gaaaggaagg ccctgaattc
     7861 ccggccgtga tgaatctccc gcgggatatt cgcaatcgcc agcgcattta agagcgggat
     7921 taccatcaag ggcggaaaag ccttcaaatt atcggacatg tgagggacat aaagcagcgt
     7981 ggccatggtt gtcagtatat aacatattat aaagaagatt atagttggat taacccatgc
     8041 cctgatcttg ctctgtagtt caccctcagt tttcatcagg aggtatatgg ccccatgcat
     8101 catgaataaa gccacggtgg tgataccgac aagaagtgca tagggatgca gaaggcggat
     8161 gaatctgccg gcgtactcaa aatccccatc gagcggaatc ccccaggcta tgttccccag
     8221 cgcgatgccg atcaggaggc tcgacaggat actggaaaag ctgaagctta tatcccagaa
     8281 ttggcgccag gttgtgcttt ccctcttgct tctaaaatca atcgccacgg ccctgaagat
     8341 caaccccagc aggagcagca taattgccat ataaaagccc gagaatgcgg tggcatagac
     8401 ttccggaaat gccgcaaaga gcgcaccccc gccggtcacc agccagacct cattaccatc
     8461 ccagaccggg ccgatggcgt taagcatgat gcgtctttct ttatcggtac tggtaaacag
     8521 atgtaatgcg ccggtgccca gatcaaagcc gtccagaatg gcgtatcccg taaaaagcac
     8581 acccacaaga ataaaccaga ttgtattgag atcgagactc atgccatatg cccctcttta
     8641 agttcgtact tctccggccc gcccttgatc
//
LOCUS       DSEB01000360            8679 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000405, whole genome shotgun sequence.
ACCESSION   DSEB01000360 DSEB01000000
VERSION     DSEB01000360.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8679)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8679)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8679
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000405"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..719
                     /gene="sufB"
                     /locus_tag="ENO07_06400"
     CDS             <1..719
                     /gene="sufB"
                     /locus_tag="ENO07_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDS01634.1"
                     /translation="IEGCTAPSYSTDSLHSAVVELISLEDSYIRYTTIQNWSQNVFNL
                     VTKRAHAHKNATVEWVDGNIGSKLTMKFPCVYLLGEGAKGEILSVAFAGDGQHQDAGA
                     KVIHVAPRTSSRVTSKSISKAGGRASYRGMAKVYPGAEGSKISVECDALLLDGASRSD
                     TYPTMEIDNDNVRIEHEARVSKVAEEQLFYLTSRGLTEDEARLLIINGFIEPFTKELP
                     MEYAVELNRLIELEMEGSVG"
     gene            712..2055
                     /gene="sufD"
                     /locus_tag="ENO07_06405"
     CDS             712..2055
                     /gene="sufD"
                     /locus_tag="ENO07_06405"
                     /inference="COORDINATES: protein motif:HMM:PF01458.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDS01635.1"
                     /translation="MAKLKIINDQGADMITETLTEQKVSFRPIAENEPEWLYDLRIRA
                     WNYFQEAEMPSQVEHLWKYTKPKWFMPDGLEQKMSVLPVVSEIKDEEIAPLKPEFAAF
                     GCNHGDRVTYTQLSDELKSSGIIFEDIQKAINNHGELVRKYLGQLIGYDFGKFEALNM
                     ALFNSGMFLYIPPDMELGKPIYMHRHPDDKYNMPRLLVIVGDNSRATIIDDYAGKSND
                     KPYNANSAVEIFGGQNSQIRFVSPQNLYKKSRSYVTQRARIGRHARMFSIFVSLGADV
                     SKYNLGTILNGQGAYSQMLGILFGDDNQHFDHHTMHHHKAGESFSNIDFKVVLKDKAL
                     SAYTGLIKIDENTLNCEAFQENRNLLLNKGARAESIPELEILNDQVRCSHGATVGPID
                     PEMVFYLKSRGYSTDDAVRAIVTGFIEPLLKSVPDDLRQMMQNMVQFKLRNGTGE"
     gene            2204..2698
                     /locus_tag="ENO07_06410"
     CDS             2204..2698
                     /locus_tag="ENO07_06410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDS01636.1"
                     /translation="MRISSRGINSMDSNLNELYRDILMDHYKYPRGKKTLDNPDLENQ
                     GQNPLCGDSLTVQVKISDSVIEDVGVSCVGCAICTASSSMLADIIKGKTLDEVKEIAR
                     VVKHLLKGDDFESDLDLGDLEALEGVKKFPVRIKCALLSWTTLIDSIESWEKGAELKV
                     ISTE"
     gene            2704..3009
                     /locus_tag="ENO07_06415"
     CDS             2704..3009
                     /locus_tag="ENO07_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004431774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF59 domain-containing protein"
                     /protein_id="HDS01637.1"
                     /translation="MITPEEIREKLAPIQDPEIGLGVVDLGLIYDIDVSDDGKVKILM
                     TLTTPACPYGPELVDAVRIATEKMEGVTEAEVEVVWEPQWNPEEMASDYAKDVLGIW"
     gene            3130..4221
                     /gene="pdhA"
                     /locus_tag="ENO07_06420"
     CDS             3130..4221
                     /gene="pdhA"
                     /locus_tag="ENO07_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDS01638.1"
                     /translation="MLLKTFDPLKGKQLRIMDENGEIVDEKNMPELTDDQVVEAYKYM
                     LYARQVDLKAVAYQRMGRLFTLPPNLGQEAAAFGSAYALDKKKDWMVPAFREMAAWLY
                     WGATAKDLYLYWAGSEDGNVTPDDVNFMPSAVPVASQVPHAAGLGYSIKYKGTDQVVI
                     TYFGDGGTSEGDFHEGMNFAGVFKLPVIFFCNNNQYAISVPRRMQSAAKNLADKAIGY
                     GMPGIQVDGNDFFAVYAATKEAAEYARDGNGPVMIEAETYRRGYHTTSDDPTKYRTAE
                     EEAEWEKKDPLIRMKKYLEKKGLWDDDKENDYVKHAKKDISAQFKEVEEYPRTSLEQI
                     FKYMYEEMPDILKKQKVEMEKYLAGKEGR"
     gene            4222..5199
                     /locus_tag="ENO07_06425"
     CDS             4222..5199
                     /locus_tag="ENO07_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016338687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDS01639.1"
                     /translation="MAVMNMVQAINSALDFKLKDDESVVVYGEDVGLEGGVFRVTEGL
                     QKKYGEKRVFDTPLAESMIIGTGVGMAAGGLRPVAEIQFSGFIYPGFNQIISHVSRMR
                     NRSRGTITCPMVIRAPYGGGVRALEMHSESLEAVYGHIPGLKVVMPATPYDAKGLMIA
                     AIEDNDPVMFLEPKKVYRAIKQEVPEDPYRIKIGKAKIAQEGEQITVVAYGAMLRTAQ
                     QGIAKAKKDGISVELIDLRTIFPMDTDTIIESVKKTGRILIVHEAARSFGVAAEIMSL
                     VNEEAFLYLEAPPTRLTGFDTIVPFPMGEDLYFPDADRVYHHIKKVVEF"
     gene            5209..6576
                     /locus_tag="ENO07_06430"
     CDS             5209..6576
                     /locus_tag="ENO07_06430"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00198.21,HMM:PF00364.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDS01640.1"
                     /translation="MLYVFKFPDIGEGIHEGKILKWHVKKGQEVMEGDPVVNVETDKV
                     VTDIPIPKDGVIKNTYGKEGEVINVGDALVEMEVEGEEAEEEAEVKAPPKEGREAEGE
                     PASRTEVAKAKTKTKKIEEKGFGVVGQIEEADTDAFLPATGEGMEAQAAVAAEKEPGR
                     KALATPVARKMAMDLGIDINTVRGTGPAGRVMKEDIKKAFDEKQAGPKVARKPGKIEI
                     PAEAEDMVEVEELTQIRKTIINRMVQSKFTAPHATAFEEVEVSRLVELRKEKKEELAE
                     QGVKLSYMPFIIKAVSLALRRHKSLNCKLDMENNRVIYQKFYNIGFATDTPEGLMVPV
                     IKNADRKSIIEIAESIKDLSDRARERKIQLDELKGGTFSITNYGSIAGTYGVPVINYP
                     EVAILGVGRIVQRPVVKGGEIVPGNVLPLSMSFDHRIVDGADAARFIKDLMAMLADPL
                     TMLMM"
     gene            6586..7935
                     /gene="lpdA"
                     /locus_tag="ENO07_06435"
     CDS             6586..7935
                     /gene="lpdA"
                     /locus_tag="ENO07_06435"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDS01641.1"
                     /translation="MTTFDLIIIGGGPAGYVGAIRAAQLGMKTAVIEKSRMGGMCLNW
                     GCIPSKAFIESAKLFKKIANAKSFGIDGVDKKALKFNWKKAAQRKDRIVMRLVKGVEY
                     LMKKNGVEVSNGEARILDKEHVGVGDTKYTCDKMLIATGSRPLRAEYKGIDQKKIMEI
                     DEFFALEEKPECVLVDGGRINACEMALMLRYTGSKVTMVTESESLIPFLDEEIRKFVM
                     DKIEKAGIKVYTRSEITKDAQDGVYVGDNFVECDIILNAKRRRAILPEMGELELEMNG
                     HFLQINEYMQTSVPNVYAAGDVTKQFFAQVASAQALTAVNHMNDIKETLDYNKLPVNI
                     YTDPEIAYVGLNEEQLKEKGIGYKVGKFPLSVNGKAMIEGETEGFVKVLSETKYGEVM
                     GVHIVAADATDMIAEAVMAMRLESTVDELARVVHAHPTISETFMEASYVAADRPVHI"
     gene            7942..8565
                     /locus_tag="ENO07_06440"
     CDS             7942..8565
                     /locus_tag="ENO07_06440"
                     /inference="COORDINATES: protein motif:HMM:PF03099.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01642.1"
                     /translation="MSIKISEDYNLPDSEMLRSGNSYKGLMVWVPENLMVVVGKGSDP
                     EEELLAENILLDRIPVIRRGTGGCAVVLSPEMAVVSLILENDPDKKQNEYFRQFVGVI
                     TNAFKKMGVENIEMKGISDIAINGLKVAGSSIFRSKDYVFFHSIINIAGNTRAMERYL
                     KFPPRQPDYRLNRSHRDFVTSLKEQGYEIDIHEFEENLKSEWLLHFR"
BASE COUNT         2376 a         1888 c         2423 g         1992 t
ORIGIN      
        1 atatagaggg ttgtacagct ccttcatact caacggattc actgcattcg gcggtggttg
       61 agctgatctc gctggaagat tcctacatac gatacacaac catccagaac tggtcgcaga
      121 atgtgttcaa tcttgtgacc aagagagccc atgcgcacaa gaacgcgacg gtggagtggg
      181 ttgacggtaa tatcggctcc aagctgacca tgaaattccc ctgtgtctat ctcctgggtg
      241 agggcgccaa aggtgagatt ctgtcggtag cgtttgccgg ggatggccag catcaggatg
      301 ccggcgccaa ggtgattcat gtcgcccccc ggaccagctc cagggtgacc tccaagtcca
      361 tctcgaaggc gggtggaaga gcttcctaca ggggcatggc caaggtatat cccggtgccg
      421 agggaagcaa gatatcggtg gaatgcgacg cccttcttct ggacggcgcg tcgaggtcag
      481 atacctatcc gactatggag atagataatg ataatgttcg catagaacac gaggcccggg
      541 tcagcaaggt tgccgaggag cagttgttct atctgacctc gcgcggcctg accgaggatg
      601 aggcgcgcct gctcattatc aacggcttta tcgagccgtt cacaaaagag cttccgatgg
      661 agtatgctgt tgaattgaac aggcttatcg aactggaaat ggaaggctcg gttggctaaa
      721 ctgaaaatta taaatgatca gggtgcagat atgataacag aaacattgac agaacagaag
      781 gtctccttca ggccgattgc ggaaaatgag ccggaatggc tctatgatct cagaattcgc
      841 gcctggaact atttccagga agcggagatg ccctcgcagg tcgagcatct ctggaaatac
      901 accaaaccca aatggtttat gccggatggc ctggagcaga aaatgtcggt ccttccggtg
      961 gtttcagaga taaaggacga ggagattgcg ccattaaagc ccgagtttgc ggcatttgga
     1021 tgcaatcatg gcgatcgggt tacctataca cagctttccg acgaacttaa atcatcgggg
     1081 attatatttg aggatatcca gaaagcgatc aataatcatg gcgagctggt gcggaaatat
     1141 ctggggcagc tgatcggcta tgatttcggc aagtttgagg cgctgaatat ggccctcttc
     1201 aacagcggga tgttccttta tattcctccc gacatggaac tgggaaaacc gatttacatg
     1261 catcgccatc cggatgacaa atataacatg ccgcgtctcc tggtcattgt gggggataat
     1321 tccagggcga ctataatcga cgactatgcc ggcaaatcga atgataagcc atacaatgcc
     1381 aatagcgcgg tggagatatt tggcgggcag aattcacaga tacgctttgt cagtccccag
     1441 aatctctaca agaaatcgcg ctcttatgtt acccagcggg cacggatcgg ccggcatgca
     1501 aggatgttct cgatttttgt gtctcttggg gccgatgttt ccaagtacaa tctcgggaca
     1561 attctcaacg gtcagggcgc ctacagccag atgctgggta tattattcgg tgatgacaac
     1621 cagcatttcg atcatcatac catgcatcat cacaaagccg gagaatcatt ctcaaatatt
     1681 gatttcaagg ttgttcttaa ggataaggcg ctgtcggctt ataccgggct tatcaagatc
     1741 gatgagaata cattaaattg cgaggcgttt caggaaaata gaaacttact tttgaacaag
     1801 ggcgctcggg cagaatcgat tccagagctt gagattttga atgatcaggt gcgctgcagt
     1861 catggcgcaa ctgtcggccc gattgatccg gagatggttt tctacctgaa gagccggggt
     1921 tattcgactg atgatgcggt tagagcaatc gtaacgggct ttattgagcc cctgttgaaa
     1981 tccgtacctg atgatttgcg ccagatgatg caaaatatgg tgcaattcaa gctgagaaac
     2041 ggaaccggag aataaaaagc ttgttgttta atcaaaatgg atagtgacaa acataaaaga
     2101 tggtgcgggc gcaagaatag tacatctcgc actggcatta agcccgcgcc atttgattta
     2161 aaaggtaaga tggggcatga aatgataaaa tctctgtctt caaatgcgca tctcctcaag
     2221 gggaatcaat tccatggata gcaatcttaa tgaattatac cgggatattc tcatggatca
     2281 ctacaagtat ccgcgaggga agaagaccct ggataatccc gatctcgaaa atcagggaca
     2341 aaatccgctt tgcggggact cgcttaccgt gcaggtgaaa atcagtgaca gcgtgatcga
     2401 ggatgtgggc gtaagctgtg tcggatgtgc gatctgtact gcctccagct cgatgctggc
     2461 ggatatcatt aaaggtaaga cactggatga ggtcaaggag atcgcccggg tggtcaagca
     2521 tcttttgaag ggcgatgact ttgaatcgga tttggatctt ggggatctgg aggcattgga
     2581 gggcgtgaag aaatttccgg ttagaatcaa atgtgctctt ctttcctgga caaccctgat
     2641 cgacagtatt gaatcatggg agaagggtgc agaattgaag gttataagca cggagtgaat
     2701 gctatgatta ccccggaaga gattagagaa aagctggcgc caattcagga ccctgagatc
     2761 ggcctgggag tggtcgatct gggtcttata tatgatatcg atgtttctga tgatggtaaa
     2821 gtgaagattc tgatgaccct gacaactcct gcctgcccgt acggcccgga gcttgtcgat
     2881 gctgtcagga ttgctactga gaagatggaa ggagtgacag aagctgaagt tgaggttgtg
     2941 tgggaaccgc agtggaatcc cgaagagatg gcctccgatt acgccaagga cgttctcggt
     3001 atctggtaaa attcaattga atttacgggg agatggatta ttttgacgaa aacttatata
     3061 ccgattctgg agtcggaata atactatagt gaaaaatcaa tgatgtttac gaaaggatag
     3121 aggtacccga tgttgttgaa aacgtttgat ccgcttaagg gcaaacagtt gcggatcatg
     3181 gacgagaatg gcgagatagt ggatgagaaa aatatgcccg aactgactga tgatcaggtc
     3241 gtcgaggcat acaagtatat gctctatgcc cgccaggttg atcttaaggc tgttgcctat
     3301 cagcgaatgg gaaggctttt tactctgccc ccgaatcttg gacaggaagc tgccgcattc
     3361 ggcagcgcct atgctctgga caaaaagaag gattggatgg ttccggcgtt tcgtgagatg
     3421 gcggcctggc tgtactgggg cgccacagcc aaggatctgt atctttactg ggccggtagc
     3481 gaagatggca atgttacccc tgatgatgta aacttcatgc ccagtgcggt tccggtagcc
     3541 tcacaggtgc cgcatgccgc gggtctcgga tattccatca aatacaaggg cacggatcag
     3601 gttgtaataa catatttcgg cgatggcgga acctcggaag gcgattttca cgagggaatg
     3661 aactttgccg gtgtatttaa actgccggtc atatttttct gcaataataa ccagtatgcc
     3721 atttcagtgc cgagaagaat gcagtccgcc gccaagaacc tggcggacaa ggcaatcggg
     3781 tacggcatgc cgggtattca ggttgacggc aatgatttct ttgctgtcta tgcggccaca
     3841 aaagaagctg ccgaatatgc ccgcgatggc aacggccccg ttatgatcga ggccgaaaca
     3901 taccgccgcg gttatcatac gacttccgat gatccgacca agtatcgtac agccgaggaa
     3961 gaggccgagt gggaaaagaa agatcctctt atccggatga aaaaatatct ggagaaaaag
     4021 ggcctctggg atgatgacaa agaaaacgac tatgtcaagc atgccaagaa agatatcagc
     4081 gcacagttta aggaagttga ggaatatccc cggacatcgc tggaacagat ttttaagtac
     4141 atgtacgagg agatgccgga tatactcaag aagcagaagg tggagatgga aaagtatctg
     4201 gccggaaagg agggccgtta aatggcggta atgaatatgg tacaggccat aaattcggct
     4261 ctggatttca agctcaaaga tgatgaatct gttgttgttt atggcgagga tgtcggcctc
     4321 gagggcggcg ttttccgtgt aaccgagggt cttcagaaga aatacggcga aaaaagggtt
     4381 ttcgatacac ctctggcaga atcgatgatc atcggcaccg gggtcggaat ggccgcaggc
     4441 gggctccgcc cggtagcaga aattcagttt tccgggttta tatatcccgg tttcaaccag
     4501 ataatttcac acgtttccag aatgcgcaac cgttcccgcg gcacaattac ctgccccatg
     4561 gtaatccgtg ccccctatgg cggcggggtg cgggcattgg aaatgcacag cgagtcactc
     4621 gaggctgtat acgggcacat tcccggtttg aaagtggtta tgccggcgac accctatgac
     4681 gccaagggcc ttatgattgc ggctatagag gataatgacc cggtcatgtt cctggaaccc
     4741 aagaaagtct atcgtgcgat caagcaggag gtccccgaag atccatatag aattaaaatc
     4801 ggaaaagcca aaatcgctca ggaaggtgag cagattacgg tcgtggctta tggcgcaatg
     4861 cttcgcacag ctcaacaggg aatcgccaaa gccaagaagg acggtatttc ggtcgagcta
     4921 atcgatttga gaacgatctt cccgatggat actgatacga ttatagaatc tgtcaagaaa
     4981 acgggccgaa ttctgatagt ccacgaagcc gcccgcagtt ttggggtggc agccgaaata
     5041 atgtccctgg taaacgaaga ggcgtttttg taccttgaag cgcccccgac ccgactgacc
     5101 ggttttgata cgattgttcc gtttccgatg ggtgaagatc tgtatttccc tgatgcggat
     5161 cgcgtctatc atcatatcaa gaaagttgtg gaattttaag gaggatttat gctgtatgta
     5221 ttcaagtttc ccgatatcgg tgaaggcatt cacgaaggca aaatcctcaa atggcacgtg
     5281 aaaaagggtc aggaggttat ggagggcgac cccgtagtca atgtcgagac cgataaagtt
     5341 gtaactgata ttccgattcc caaagacggt gttatcaaaa acacctacgg taaagagggc
     5401 gaagttatca atgtcggcga tgccctggtt gaaatggaag tcgagggcga agaggccgaa
     5461 gaagaagccg aagttaaggc tcctccaaaa gagggacgtg aggcggaagg cgagcccgct
     5521 tccaggacag aagtggccaa agctaaaacc aaaacaaaga agatcgagga aaaagggttt
     5581 ggcgtcgtgg ggcagattga agaggctgac accgatgcat ttcttccggc taccggcgag
     5641 ggcatggagg cccaggcagc cgtggcagcc gaaaaagagc ccggaagaaa ggctttggca
     5701 acaccggtcg cgcgcaaaat ggccatggac cttggcatcg atataaatac tgtccgcggt
     5761 acaggtcccg ccggccgtgt gatgaaagaa gacattaaaa aggccttcga tgaaaaacag
     5821 gccggtccca aagttgcccg gaagccgggc aagatcgaaa ttcccgccga ggccgaagat
     5881 atggtcgagg tcgaggaact gacccagatc aggaagacga tcatcaatcg catggtgcag
     5941 tccaagttca ccgcgcctca cgccactgca tttgaggagg ttgaggtctc gagactggtc
     6001 gagcttcgta aagagaaaaa ggaagaactg gcagagcagg gcgtcaagct gtcatacatg
     6061 ccatttataa tcaaagcggt atcgttggca ttgaggcgcc ataagagcct gaactgcaag
     6121 ctggatatgg aaaacaaccg ggtgatctac cagaagttct ataacatagg ctttgccact
     6181 gatactcccg agggcctcat ggttcctgtg atcaagaatg ccgaccgcaa aagcatcatt
     6241 gaaatagcgg aatcgatcaa agatctttcc gacagggcgc gtgagcgcaa gatccagctc
     6301 gatgaactca aaggcggaac cttttcgatc accaactatg gttcgatcgc gggcacctat
     6361 ggtgttcccg tgataaatta tccggaagtg gccatactgg gcgtgggacg aattgtccag
     6421 cgtccggtgg tcaagggcgg cgaaattgta cccggcaatg tcctgccgtt atccatgagc
     6481 ttcgatcacc gaattgtgga tggcgcagat gctgccagat ttatcaagga tttgatggct
     6541 atgctggccg atcctttgac catgttaatg atgtaggagg cagtcatgac gacatttgat
     6601 ctgataatca taggcggtgg ccccgcaggg tatgtgggtg ctatccgtgc cgcacagctg
     6661 ggtatgaaaa cggcggtaat cgaaaagtcc aggatgggcg gtatgtgcct taactgggga
     6721 tgcataccct ccaaggcatt catcgaatcg gccaagcttt tcaagaagat agccaatgcc
     6781 aaatcatttg gaatcgatgg tgtggacaag aaagccctga aattcaactg gaagaaggcg
     6841 gcccagagaa aggaccgtat agtgatgagg ctggtcaagg gcgtggaata cctgatgaag
     6901 aaaaacggag ttgaggtttc aaacggtgag gccaggattc tggataaaga gcatgtaggg
     6961 gtgggcgaca caaaatacac ctgcgacaag atgctgatcg ccacaggatc gcgtccattg
     7021 agagccgagt ataaaggcat cgatcagaaa aagatcatgg aaatcgacga gttttttgcg
     7081 ctcgaagaga aaccggaatg cgttctggtc gatggagggc gtatcaatgc ctgcgaaatg
     7141 gcattaatgc tgcgctatac cggcagcaag gtgactatgg taaccgagag cgagagcctg
     7201 ataccgtttc tggatgagga aattcgcaag tttgtgatgg ataaaattga aaaggccggg
     7261 attaaagtct atactaggtc agagattacc aaagatgccc aggatggagt atatgtcggc
     7321 gataacttcg tcgaatgcga tattatcctg aatgccaagc gccggcgtgc gattctgccg
     7381 gaaatgggcg agcttgagct cgagatgaac gggcattttc tgcaaatcaa cgaatatatg
     7441 cagacctctg tgcccaatgt ttatgccgcc ggtgatgtta ccaagcagtt ctttgcccag
     7501 gtggcctcgg cccaggccct gaccgcagtc aatcatatga acgatatcaa ggagactctt
     7561 gactacaaca agctgccggt taatatttac accgacccgg agatcgccta tgtcggcctg
     7621 aacgaggagc agttgaaaga gaagggaatc ggatacaaag taggtaagtt tccgctttca
     7681 gtcaatggca aggccatgat cgaaggcgaa accgaaggct ttgtcaaagt gttatcggaa
     7741 accaagtatg gcgaggtaat gggtgtgcat atagtcgcag ccgacgctac cgatatgata
     7801 gccgaggctg tgatggcgat gcgtctcgaa agtacggttg atgagctggc gcgtgttgtc
     7861 catgcccatc ctacaataag tgaaacattc atggaagcca gttatgtggc cgctgaccgt
     7921 ccggtgcata tttaaaatgc gatgagtatc aagatttccg aagattataa tttacccgat
     7981 tcggagatgc tccggtcggg caattcctat aaggggctga tggtctgggt gccggagaat
     8041 ctaatggttg tggtcggcaa aggctctgat cctgaagagg aactgctggc agagaatatt
     8101 ctgctggacc ggataccggt catacggcgc gggactggag gatgcgcggt tgtgctgtcg
     8161 cccgagatgg cggtcgtgtc gcttatcctc gaaaatgatc ctgacaaaaa gcagaatgag
     8221 tatttccgtc agtttgttgg agtcatcaca aatgctttca aaaagatggg agtcgaaaat
     8281 atcgagatga agggaatctc ggatattgcg attaacggtc tcaaggttgc gggatcatcg
     8341 atattcagga gtaaggatta tgtcttcttc cattcgatca tcaatattgc cggcaatact
     8401 cgtgccatgg aacgttatct caaattcccg ccccgtcagc cggattaccg cctgaaccgt
     8461 tcccacagag atttcgtaac atcactcaaa gagcagggct acgaaataga catccatgaa
     8521 ttcgaagaaa acctcaaaag cgagtggttg ctgcattttc ggtagggggc taattccgag
     8581 caatcatgtc aaatatgttc tctaacattc cagcatatcc ctctgaacat ttcccttccc
     8641 cgaccgttta gtagactatc atacaaagac aaataagtc
//
LOCUS       DSEB01000361            5300 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001153, whole genome shotgun sequence.
ACCESSION   DSEB01000361 DSEB01000000
VERSION     DSEB01000361.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5300)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001153"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            163..236
                     /locus_tag="ENO07_06445"
     tRNA            163..236
                     /locus_tag="ENO07_06445"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:197..199,aa:Met,seq:cat)
     gene            complement(263..1384)
                     /locus_tag="ENO07_06450"
     CDS             complement(263..1384)
                     /locus_tag="ENO07_06450"
                     /inference="COORDINATES: protein motif:HMM:PF00589.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01643.1"
                     /translation="MARPYKRGNKWYIYVTHKGQRVRKSIGTSKALAQKYQSMIETDL
                     MRGELKLDVSDCSIERLFTDYLEYSAINHRPSTTRRYEGAIRYFHIFLGHFYPRVKRV
                     GQLSLNVFEKYKHFRRTTDPKSINAQLSGDMRYPANCTVAKTKTVNYEIKTLKSVFEF
                     GIRRGLCSVNPARGVSLLRIDDAKQPRFLSGDECQILLENAGSEYPIYFTLLNTGLRL
                     GELLNLQWSDINFSRKVLMVRGKSDWKPKAGEREIPLNQGVLNLLRDIKPKRARKSDY
                     VFQFKRRDTAARILRKRLARLCREHGLEAFSLHDLRHTFASQLVMNGVDLATVQQLLG
                     HRDIQTTMIYAHLAPDHLAGAVRKLDSILPQICHKRGKT"
     gene            complement(1580..2218)
                     /locus_tag="ENO07_06455"
     CDS             complement(1580..2218)
                     /locus_tag="ENO07_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01644.1"
                     /translation="MDESGNFDFSGREGRQYFIITGLSVFRPFPCFEELLEYRHDLWE
                     SGQEIEYFHANNDQPHVRKKVFEIICKQLGKLQADSIILEKRKTYPQLQKDRERFYKT
                     FLGILLKYILKGHTWHNIKDIVMIHDRLAPEKRKRLSDAIKDALFFWADDVDGNYYIN
                     FIQSKASFLLQIVDYINWAIARKWEYGDQSFYDLIQPCIKSERDIFNWWKYK"
     gene            complement(2238..2678)
                     /locus_tag="ENO07_06460"
     CDS             complement(2238..2678)
                     /locus_tag="ENO07_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01645.1"
                     /translation="MRFIPMDDIKRNHIAKKWKWRYLRGIQCVLLATRGLISPNREFT
                     RAAFGSSFNEFLEILSMPDRYIIYREDYRNNGADQWRRLFRRLRPHKREEFLNLLEEL
                     NKSYRDRKKRIAKFNGLFGKLLEHYYPNGETPQRKPKQDSHFQV"
     gene            complement(2656..3654)
                     /locus_tag="ENO07_06465"
     CDS             complement(2656..3654)
                     /locus_tag="ENO07_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01646.1"
                     /translation="MRNILLAEPDYRSKFPPLGLMRLSTYHKSEGDNVTFVRGRNPDL
                     RSVNWHRIYISSLYTWELPRTLKTIKYYGTAVNNPKDIIVGGTAVTLLPQYIRSKTNV
                     TVIEGLLDRPNMLGIGSKAIAKLIPDYSIIEAVPDYEYFPADAYFTKITKGCIRSCKF
                     CAVPILERKFGFLKDLRRQIEDVNERHGEKQNLVVMDNNILGIDRIQSIFDDIAKVGF
                     AAGAMKNGKKRSVDFNQGLDARLINKDPSLAEGLASICTSPVRLAFDFVGMERPYRRA
                     IKILRQNGFNEFTKLYALQLQRCPRRSVQTNLDKCGTEFRIGNQDHRIPDEIHTHG"
     gene            complement(3654..5222)
                     /locus_tag="ENO07_06470"
     CDS             complement(3654..5222)
                     /locus_tag="ENO07_06470"
                     /inference="COORDINATES: protein motif:HMM:PF13589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01647.1"
                     /translation="MMSSNSEKMPKIGAFVLETLTTGMYTNSLDTLREYIQNSFDSIR
                     ESERSNKLTYGAGRIDLTIDEKSRILKLRDNGLGIPKNEVKSRLVNIGMSSKTIQHYA
                     GFRGIGRLAGIAYCDRLIFTTQAKDENEFSRVVFNCRELRNAMSPKLKQISELAEVIN
                     EHASVTNEKTRNRGHFFEVLMEGINEAGQSFLDWKIIENYLSQIAPVGLDTQSFYHAG
                     EIIQWLKKRNIDVPTVSITINAGLMKRQVFKPYRKLTYTTAQEKHKIHLKKIRFFPDD
                     AGPDSPFWGWYAETNCPGTIGDERVAGIRLRKDNIGIGMAENMTKIFAEASESYARLN
                     KYFMGEIHIQDPNIIPNARRDGFEEGPEWLKVRQSLVEFAKERSREAYHLSQARNLDI
                     ERLLGAAKREKESAQKRKKTGLASKEEKEKVLGKLDRQIAKLEAAKKSDRDENERKQI
                     DRTYKELQNTRQAIENETHYVAQNLNSALDKKQRKIISEIIGILYDVLDDGSFEKARA
                     AILAKYQIPNKKRE"
BASE COUNT         1327 a         1245 c         1111 g         1617 t
ORIGIN      
        1 gtcccgggga agccgttcga gaaggaattc aggcggccgg ggaacaattc gcaggctaag
       61 gaatatatat aatctggcgg gttcaaatct gcttgacttt gcccgataaa ttaatagatt
      121 gtaaaatctc tctggcagaa attgagggat ttagactaat atggcggtgt agctcagttg
      181 gttagagcag cggaatcata atccgcgtgt ccggggttca agtccctgca ccgctatttt
      241 gttttttgac aattacttag atttaggtct taccccgttt gtggcagatt tgtggcagaa
      301 tggaatcgag ttttctgacc gccccggcca aatggtcggg cgccaggtgg gcatagatca
      361 tggtggtctg gatatcccga tgcccgagca gctgctgaac agttgccaga tcgaccccgt
      421 tcatcaccag ctgactggca aaagtatgcc tgagatcatg caggctaaaa gcctcaagac
      481 catgctctct gcagaggcgt gccaggcgct ttctcaatat ccgagcggcc gtgtcccggc
      541 gcttgaattg aaagacataa tcggacttcc gggcccgttt gggcttgata tctctaagca
      601 aattcaagac cccttgattg agtggtatct ctctttctcc ggccttgggt ttccagtcgc
      661 tcttgccccg caccataagc accttccgtg agaaattgat atcgctccac tgcaagttca
      721 gaagctcccc caggcgaagc cccgtattta ataaggtgaa ataaatgggg tattcactac
      781 cggcattctc cagtaaaatc tgacattcat ctccggacaa aaaacgcggc tgcttagcat
      841 cgtcaatcct gagaagcgaa acccccctgg ccggatttac gctgcacagg cctctcctga
      901 ttccaaattc aaaaactgac ttcagggttt tgatttcata attgacggtt ttggttttcg
      961 cgacggtgca gttggctgga tatctcatgt ccccggagag ctgagcattg attgactttg
     1021 gatcggtggt gcgtctgaaa tgcttatatt tttcgaagac attcaggctg agctggccaa
     1081 cccgttttac ccggggataa aagtgaccga ggaatatatg gaaatatctg attgccccct
     1141 catagcgcct ggtggtggag ggccgatgat tgatggcaga gtactccagg taatcggtga
     1201 aaagcctttc gattgagcaa tcggatacat ccagcttcaa ttctccccgc atcagatctg
     1261 tttcaatcat gctctgatat ttctgggcga gggccttgga ggtaccgata gactttcgga
     1321 cgcgctgacc cttatgggtg acgtatatat accacttatt tccgcgtttg tatggccggg
     1381 ccatgtatca gtcaacctgc tttctttcaa agacttgcat tttccataaa aatttcataa
     1441 aaaattacta aaaaatactt gaacatttgg atttatttcc gttattatat atagacgacg
     1501 tcgtactgat tgaaaagtta ttgttccggc cctgatgatg tttgcccttt gtggcacccg
     1561 aaaggtagtc agggccattt tatttgtact tccaccagtt gaagatatca cgttcactct
     1621 taatacacgg ctggattagg tcataaaaac tctgatcgcc atattcccat tttcgggcta
     1681 tagcccagtt tatataatcc acaatctgca aaagaaagct ggctttggac tggatgaaat
     1741 ttatataata attgccgtcg acgtcgtccg cccaaaaaaa gagggcatcc tttatcgcgt
     1801 ccgagagcct ctttctcttt tctggtgcaa gtcgatcgtg tatcattact atatccttta
     1861 tattatgcca ggtatgaccc ttaaggatat atttgagcag aatgccgaga aatgttttat
     1921 agaatctctc gcgatccttc tggagctgcg ggtatgtttt tcgtttttct aagattattg
     1981 agtccgcctg gagtttacct aattgtttgc atataatttc aaacactttt tttctaacgt
     2041 gaggctgatc attatttgca tgaaagtatt ctatttcttg accggactcc cacaagtcat
     2101 gtcgatattc aagaagctcc tcaaagcagg gaaacgggcg gaaaacagac aggcccgtaa
     2161 tgataaaata ctgccgaccc tctctccctg aaaaatcgaa gtttccactt tcatccaaaa
     2221 aaatatagag catctgatca aacttggaaa tggctgtctt gtttaggctt tcgctggggc
     2281 gtttcgccat ttggatagta atgctccagc agctttccaa atagcccatt gaattttgca
     2341 atccgttttt ttctatcgcg atagctcttg tttaactctt cgagaagatt cagaaattcc
     2401 tccctcttat gtgggcggag cctccggaag agccggcgcc attgatcagc gccattattc
     2461 ctatagtcct ccctgtaaat aatgtatcta tccggcatgg aaagaatctc aagaaattca
     2521 ttgaaagaac tcccgaaagc agcccgggtg aattcgcgat ttggacttat aagcccccgg
     2581 gttgctaaga gaacacactg gatgcctctt aaatatctcc atttccattt tttagctata
     2641 tggtttctct ttatatcatc catgggtatg aatctcatcg ggaatccggt gatcctgatt
     2701 cccaattcgg aattcagttc cgcatttatc caaattcgtc tgtacagatc ttctggggca
     2761 tctttgaagt tgaagagcat ataattttgt gaattcattg aacccgttct gcctgagaat
     2821 cttaatcgca cgccgatatg gccgttccat gccaacaaaa tcgaacgcca gtcggacggg
     2881 agaagtgcat atactggcta atccttcagc aagcgacgga tccttattaa tcaggcgcgc
     2941 gtccaatccc tgattaaagt cgactgaccg ctttttaccg tttttcattg cacctgcggc
     3001 aaagccgacc tttgctatat catcgaagat tgattggatt ctgtcaatac caagaatatt
     3061 attatccatc acgacaagat tctgcttctc cccatgcctt tcattgacat cttcaatttg
     3121 tcttcttaga tcctttaaga acccgaattt tctttccaat atgggcacag cgcaaaattt
     3181 acaggagcgt atacagcctt tggtgatctt ggtaaaataa gcatctgctg ggaaatattc
     3241 ataatccggc actgcctcta tgatagaata atcgggaatg agtttagcga tggcctttga
     3301 tcctatacca agcatgtttg gtctatccag caggccctca ataactgtta catttgtctt
     3361 gcttcgaatg tattgtggaa gaagagtcac agctgtcccg cccacgatga tatccttcgg
     3421 attatttacg gcagtgccat agtacttgat cgtcttcaat gtgcgcggca attcccatgt
     3481 gtaaagcgat gagatataaa tgcggtgcca atttaccgac cgcaaatccg gatttcttcc
     3541 gcggacaaag gtaacattat ctccctcact cttatgatac gtggataatc tcatcaaccc
     3601 aagaggtgga aatttcgatc tatagtcggg ttccgcaagg agtatatttc gcattactct
     3661 ctcttcttgt ttgggatttg atattttgcc aggatcgcag cacgggcctt ctcgaatgat
     3721 ccatcgtcca acacatcata aagaattccg attatttcag atataatttt cctctgcttt
     3781 ttatccagag ctgaattcag attctgagcc acataatggg tttcgttttc gattgcctgc
     3841 ctggtatttt gaagttcttt ataagtgcga tctatctgct tccgctcatt ctcatctcga
     3901 tcagactttt ttgcagcctc gagcttcgct atttgcctat cgagcttgcc aagaaccttt
     3961 tctttttcct ccttggaggc caacccagtc ttttttctct tttgagcaga ttccttctct
     4021 ctcttggcag ccccaagcag cctctcaatg tccaaattac gtgcttgtga taaatggtat
     4081 gcttctcggc ttctttcctt cgcgaactca accaacgatt ggcgaacttt caaccattcc
     4141 ggaccctcct caaatccatc ccgccttgca tttggtataa tattcgggtc ctgaatatgg
     4201 atctcaccca tgaagtactt attgagtcga gcgtagcttt cagaagcttc agcaaagatc
     4261 tttgtcatat tttccgccat accaatgccg atattatctt ttcgcaatcg aattcctgca
     4321 accctctcgt ctccaattgt gccggggcaa ttggtttcag cataccaacc ccagaaggga
     4381 gaatcaggtc cagcgtcatc ggggaaaaaa cgtatcttct tcaaatgtat tttgtgtttc
     4441 tcctgtgcgg tcgtatatgt cagcttgcga tacggtttaa aaacctggcg cttcattaat
     4501 cctgcattga ttgttatgct caccgtcgga acgtcgatat ttcgcttttt cagccattga
     4561 atgatttcac ctgcgtgata gaaggattgt gtatcaagac cgaccggagc tatctggctg
     4621 aggtaatttt cgataatctt ccagtccaga aatgactgtc cggcctcatt gatcccctcc
     4681 attaaaactt caaagaaatg tcctctattg cgtgtcttct cgttagtgac agatgcatgc
     4741 tcatttatca cctcagccaa ttcgctaatt tgctttaatt ttggagacat agcgttgcgc
     4801 agttctcggc agttaaatac cactcgcgaa aattcgtttt catcttttgc ctgggttgtg
     4861 aaaataagcc tgtcgcaata tgcaattcca gcaagtcgcc ctattccgcg aaagcctgca
     4921 taatgctgga tagtctttga agacattcca atattaacaa gccgggattt cacctcattt
     4981 ttaggaatgc ccaatccgtt atcacgcaat ttgagaattc ggctcttttc atctattgtg
     5041 agatctattc gaccagctcc atatgtcaat ttgttgcttc gttcggattc gcggatcgaa
     5101 tcgaacgaat tctgtatata ttccctaagc gtatcaagag aattagtgta catcccggtg
     5161 gttagggtct caagaacgaa tgcccctatc tttggcattt tttcagaatt tgaggacatc
     5221 atatttacct ctgcagctgc cgataagctg gatatgggaa acgaaccttt attaatgcag
     5281 tattaaatac agaatgctga
//
LOCUS       DSEB01000362            6955 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000648, whole genome shotgun sequence.
ACCESSION   DSEB01000362 DSEB01000000
VERSION     DSEB01000362.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6955)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6955)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6955
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000648"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1723)
                     /locus_tag="ENO07_06475"
     CDS             complement(<1..1723)
                     /locus_tag="ENO07_06475"
                     /inference="COORDINATES: protein
                     motif:HMM:PF01734.20,HMM:PF07244.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01648.1"
                     /translation="MMIKALIKSLVCILLLVSGAPAQSDTIALLNLDIFSSRAANDSF
                     SDTSVGKPKIALALSGGGARGIAQIGIIRVLENENIGIDLICGTSMGGLIGGLYSAGI
                     SIDSIENIARALDWTDFFSNRPQRSTQFITQKEYGENSIITLRFRGTDIFIPSGVTSG
                     QKLNSLLSYVTLRSDYVYENSFDSLPIPLRICAVDLVSGKQVVFDSGSLGNSIRATTS
                     FPLAFEPFEMDSMLLVDGGLLQPIPVETALEEKADFVIAVNTTSDILAFEDINDPLDI
                     INTTTTIMQLELKEKEIELADVVIKPELGDIEATDFSMVEKLIRAGEKAAAEALPEIR
                     KKLSELEGISNHDSTVIDSINLMASPFLIETESFLRPEGNGQYVLSLNAVRRHLTSAI
                     QNGVLSQAEVYITNSAMRTLHISAQPTFRLDRLVIKGTDEITEAETMKAAGLDYGDYV
                     NLTNLQLLRKTATEMMLSKGYDLAFAEIRRADDKGGTVILDINEGRISGFRISGNSRT
                     RSWVIKRNFLLKEGEPYSVARADSGLANIYGSGLFTEIRLNLEHQDEGPLVHIMVKEK
                     YSGFMRLG"
     gene            1810..2586
                     /locus_tag="ENO07_06480"
     CDS             1810..2586
                     /locus_tag="ENO07_06480"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00588.17,HMM:PF08032.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDS01649.1"
                     /translation="MDLTQRELSALRKLNSRKGRKESGRFLVEGVRCVKELVSSDYEC
                     EKIIVCRNLLTETGRNFLDSLKGFQVYELTTRKFNQITDEKTSQGIIAVARAADSGQP
                     EFEPDGLVLVIQKMSDPSNLGSIIRSAVAFGAKLIIGPQSVELYSPRVISASSGYLFK
                     AHVKTVRDILLEISTLHENSFVVWGADKNGQEIGMIEDMPARIALVIGHEAFGLADDI
                     TNELDKLVGIPISSRVDSLSAPIAASILMQRISERMGITK"
     gene            2638..3732
                     /locus_tag="ENO07_06485"
     CDS             2638..3732
                     /locus_tag="ENO07_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01650.1"
                     /translation="MKQICIYIFLLLIFVCPAGQSQAESIRTISDNNYETNESRLFLS
                     PDELPPTAELIFRSSTALRGRLEISSTDADSIEVIYHKVLYADSREQAETFASQISLS
                     HEVKENSLIISADAPRDAPWRRTANLSGQIECEIYLPRRFSISIDSVVGYYLSIRGPF
                     PQAYVAGDYYDEIRISRIDLGLTVRATNSTIILRDIQGPMLIEGEGANITARNLDTGT
                     GIGIATFENERGAISIDGFTGDELRCISREGRITLEKLSLLNGARAFISNSGINSDIY
                     VEVQEIRDSRLEIINKAADVQLLLPRDIQAEFNITTDPDDGEIEMSGVPLVTEQVDWG
                     TLIASTFHHDSRIVVDTRGTGKVTVRRKDF"
     gene            3812..6232
                     /gene="sppA"
                     /locus_tag="ENO07_06490"
     CDS             3812..6232
                     /gene="sppA"
                     /locus_tag="ENO07_06490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDS01651.1"
                     /translation="MKKAIAVLIILWLLPACLASERYPYPYYPLPEYSGAVTFGTLSI
                     FQNPAALGINPDMELMYLHSFNSDRFSGDNSILFSRNGLGLAYQNYRLSVDDAMNAFT
                     IGFSGHISQGIYAGATYKFIKAEDGNPYHNDHFWNLGLLWRYSREISLGFVAENLKGM
                     EFAGEETDIEYKLSAGFRPLQELLTLSADWSWSGGEAFSDGVFRGFFNLKIKEGIGIL
                     GSVDEDGNFGFGVNFSFGYNQIGTYHTYTNGGHDWSGLLYSGYSYRPQGWVFPVKKKF
                     LKLTLSGSYPETGEKDFFLAPERTTFLDLVQTLNRALDDEIISGIYVELRNPRLGWAQ
                     IKELRTLFHSFRNKGKAVLFYLGHLPGNASYYLAASADKIAMHRVDDLAITGLLGEVT
                     FYKGTLDKLGIEADIYAAGKYKSAADILTRDSMSRHHREAVNKLLDDIFEQYVNDLAF
                     DRDLTRDSVISLIDNAPHVSEEAVQAGLVDTILYQDQIPDWQKSRFGSSCSIDFEEYS
                     KRKPYRERWGLPPQIAVIPVEGTLMSGSSGEIPFYGKTTGSKDLAMAIEYARKNSRIR
                     AVVLRLNTPGGDGLASDLLHRELELLSKEKPLIVSMGNVAASAGYHLASAGDYIYAQP
                     STVTGSIGVVYGKLDLSDLRENIGFTTYHLKRGKNADFFSWNTGFTEQQEQKLQRQLD
                     LMYEDFVQIVAEGRDLSYDYVESVAQGRVWSGSAARELKLVDDYGGLWDALHDAKERA
                     GISDDEVEIVSFPKRPFRFLDLWSGLSSAAKTIKSLMFGNGILSDTANDSGFSSYYYI
                     LPYSIKIY"
     gene            6366..>6955
                     /locus_tag="ENO07_06495"
     CDS             6366..>6955
                     /locus_tag="ENO07_06495"
                     /inference="COORDINATES: protein motif:HMM:PF00675.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDS01652.1"
                     /translation="MLKKSILIAATGLLFLFSYLVAQDNPLGWKITKTRLDNGMNIVV
                     LEDHSTPSVAYQIWYHVGSKNERPGITGISHMVEHMMFKGTDTIGPEEHARIVQARGG
                     TSNAFTHFDMTAYHEVLGAQDLEIAAQLEADRMANLALDSAELASEKEVVAEERRTVI
                     DNSPFGPLLEQLLNLTYIAHPYHWHVIGYMSDIQNYT"
BASE COUNT         1701 a         1697 c         1730 g         1827 t
ORIGIN      
        1 acccaagacg cataaatccc gaatattttt ccttcaccat gatatgcacc agcggacctt
       61 cgtcttgatg ctccaggttc aatcttatct cggtaaaaag cccggaacca taaatgttgg
      121 cgagcccgct gtccgccctg gcaactgaat acggttctcc ctcctttaac aggaaatttc
      181 gctttataac ccagcttctg gtcctgctgt taccgctgat cctgaatccg ctgatccgtc
      241 cctcgttgat atccagaatc actgtccccc ccttatcgtc tgcacgtcta atttccgcaa
      301 atgccagatc ataccctttg gagagcatca tttccgtggc tgtttttcgc aggagctgca
      361 aatttgtgag gttgacataa tctccgtaat ccagcccggc ggccttcatg gtttcagcct
      421 cggtgatttc atctgtaccc ttgattacga gtctatccag tctgaatgtc ggttgggcgc
      481 tgatatgcaa agttctcatg gcgctgttgg tgatatatac ttcagcctgc gagagtacac
      541 cgttttgtat ggcggacgta agatgtctgc ggacggcatt gagagaaagt acatattgtc
      601 catttccctc aggcctcaga aaagattcgg tttcaatcag aaacgggctg gccatcaaat
      661 ttatgctgtc tattacagtg gaatcatgat tgcttatgcc ctctaattcc gagagttttt
      721 tccggatttc gggaagcgct tctgcggccg ctttctcacc cgccctgatc aatttttcaa
      781 ccatcgaaaa atcggtcgcc tcgatgtcgc ccagctccgg cttgattacc acatcggcca
      841 gttctatttc cttttccttc aattccagtt gcatgatggt ggtagttgta ttgataatat
      901 ccaggggatc gttgatatct tcgaaagcca gaatgtcgga ggtggtattt acagcaatta
      961 caaaatcggc tttctcttcc agggcggttt cgacaggaat gggctgcagc agaccgccat
     1021 caacaagcag catactgtcc atctcaaatg gttcgaatgc cagtggaaat gaagtggtgg
     1081 cccgaattga atttcccaga ctgccgctgt caaaaaccac ctgttttccg gagacgagat
     1141 cgaccgcaca tatcctcagc ggaatcggca ggctgtcgaa tgaattttca tatacgtaat
     1201 cggaccgcaa agttacataa gacagcaacg aattgagttt ctgcccgctc gttacaccgc
     1261 tgggaataaa aatatctgtg ccccggaagc gcagcgtgat tatgctgttc tcgccgtatt
     1321 ctttctgggt gatgaactgg gtcgagcgct gtggacggtt cgagaaaaaa tcggtccagt
     1381 ccagcgctct ggctatattt tcaatcgaat ctatcgatat gcctgctgaa tacagccccc
     1441 cgatcagccc gcccatgctt gtcccgcaga tgagatcgat ccctatattc tcattttcga
     1501 ggacccggat tattccgatc tgagcgattc ctcttgcccc tccgcccgaa agcgccagag
     1561 ctattttagg ttttcccaca gaggtatcgg aaaagctgtc atttgcagct ctgctgctga
     1621 aaatatccag gttcagcagg gcgatcgtat cggattgggc cggggcaccc gaaaccagca
     1681 gcagaatgca aaccagactt tttatgagcg cttttatcat cacgctgaaa ggttaattcc
     1741 catcgcataa aaagcaagtg attttgtcaa tattgttgac tttcctgcga ggcatgataa
     1801 tttcatccca tggatctcac tcaaagagaa ttgtctgctc ttcgcaaatt gaactccagg
     1861 aaaggacgaa aggaatccgg ccgcttttta gtcgagggtg tcagatgtgt aaaggaactt
     1921 gtttcctctg attatgaatg cgaaaaaata atcgtctgcc ggaatctttt aaccgaaact
     1981 ggaaggaatt ttctggattc tctaaaaggc ttccaggtgt atgaactgac cacaaggaaa
     2041 ttcaatcaga ttaccgatga aaagacctcg cagggtataa ttgcagtcgc tcgggcggcc
     2101 gattcgggcc agcctgaatt tgagcctgat gggctggtgc tcgtgattca aaagatgtcg
     2161 gatccctcga atctgggttc gatcataaga tctgcggttg catttggcgc aaagctgata
     2221 atcggtccgc aatcggtaga gctttactct cccagagtga tttcggcatc gtccggatat
     2281 ctcttcaaag cccatgtcaa aactgtccgg gatattttat tggaaataag cactctgcat
     2341 gaaaactctt ttgtggtctg gggggcggac aaaaatggtc aggagatcgg catgattgag
     2401 gatatgccgg cgagaatcgc cctggtgatc gggcatgaag ctttcggtct tgccgatgat
     2461 ataacaaatg agctagataa actcgtgggc attcccatct caagcagggt tgattcactt
     2521 tctgcaccga ttgccgcaag tattctgatg cagaggatct ccgagagaat gggtattaca
     2581 aaatagtatt gtcaaatcgg tttggaactt actatattag aaccgtaaac tggatatatg
     2641 aaacagattt gtatttacat atttctgctg cttatcttcg tttgtccggc gggacaatcg
     2701 caggccgaga gcatacggac aatttctgat aacaactacg agactaatga gagccgcctg
     2761 tttttgagcc ccgacgagtt acccccgact gccgaattga ttttccggtc ctccaccgcc
     2821 ttgagagggc gtctggagat ttcctcaacc gatgctgaca gtatcgaagt tatttatcac
     2881 aaggtgctct atgccgattc gcgcgagcag gcggaaacat tcgcctccca gatttcattg
     2941 agtcatgagg taaaagaaaa cagcctgatc atctccgccg atgctccccg cgatgctccc
     3001 tggcgcagga cagccaacct ttccggccag atcgaatgtg aaatttatct gccccgccgg
     3061 ttctccatca gtattgacag cgtggtgggc tattacctca gcatcagggg ccccttcccg
     3121 caggcctatg tggccggtga ctattacgat gaaatcagga tctcccgcat tgatctgggg
     3181 cttacggtca gggccaccaa cagcacgatt atactgcgcg atatccaggg tcccatgttg
     3241 atcgaggggg aaggcgcaaa tatcaccgcc cgaaacctgg ataccgggac gggtatcggc
     3301 atcgctactt tcgaaaacga gcggggagcc atttctatcg acggcttcac cggagatgag
     3361 ctgaggtgta tctcgcggga gggcaggatc actctcgaaa agctgtccct tttgaatgga
     3421 gcaagggcgt tcatatctaa ctcggggatc aatagtgata tctatgtcga ggtccaggaa
     3481 atcagggatt cccgtctgga gataatcaat aaggcggccg atgttcagct tttattgccg
     3541 cgcgatatcc aggccgagtt taatatcacc accgatcctg atgatggtga gattgaaatg
     3601 tccggcgttc cgctggttac cgagcaggtt gactggggca ccttgatcgc cagcactttt
     3661 catcatgatt cacgtatagt ggtggatacc cgcgggaccg ggaaggtcac tgtgcgccgt
     3721 aaagacttct aataatgttc ctgtctattt atttttgttg atggcatctc atcgcttttc
     3781 tatatatatg taaattgata aggaggcggt tgtgaagaag gctattgctg ttttaataat
     3841 attatggctt ctgccggcat gtctggcatc cgaaaggtat ccctatccct actatcccct
     3901 gccggaatat tcgggggctg taacgtttgg cactctctcg atttttcaga atcctgcggc
     3961 tttgggtatc aatccggata tggaactgat gtatttgcat tctttcaact cggatagatt
     4021 ttccggtgat aacagcattc tattttctcg taatggtctc gggctggcct accagaacta
     4081 ccggctatcc gtggatgatg ccatgaatgc tttcaccata ggattttcgg gtcatatctc
     4141 gcagggaatt tacgccggag caacctacaa gtttattaag gctgaagacg gaaatcccta
     4201 ccacaatgat catttctgga acctggggtt attatggcga tacagccggg aaatatcgct
     4261 tggctttgtg gctgaaaacc tgaagggcat ggaattcgcc ggggaggaga ccgacataga
     4321 atataaactt tccgccgggt tcaggccgct tcaagaactg cttacccttt ccgccgactg
     4381 gtcatggagc gggggagagg cattttccga cggtgttttt cggggattct tcaacctgaa
     4441 aatcaaagag ggcatcggca ttctcggttc agtcgatgaa gacggcaact ttggcttcgg
     4501 agtcaacttc agtttcggat ataatcagat cggcacctac catacatata ccaacggcgg
     4561 tcatgactgg tcgggcctgc tctacagcgg ttacagctat cgtccccagg gctgggtgtt
     4621 tcctgtaaaa aaaaaatttt tgaagctgac tctctccggt tcataccctg agaccggtga
     4681 aaaagatttc tttctcgctc ccgagcggac cacttttctc gatctcgtgc agaccctcaa
     4741 tcgtgcgctc gatgatgaga taatcagcgg catttatgtc gaattaagaa atccgcgcct
     4801 gggctgggcc cagatcaagg aactgcgtac cctcttccat tcgtttcgga ataaaggtaa
     4861 agcggttctg ttctatcttg gacatttgcc cggcaatgcc agctattacc ttgctgcaag
     4921 cgccgataaa atcgccatgc accgggtgga tgatctcgct atcacaggac ttttgggaga
     4981 ggttaccttt tacaaaggca cgcttgataa gctggggatc gaggctgata tctatgctgc
     5041 gggcaaatat aaatcagccg ccgatatttt gacccgcgat tcgatgtccc ggcatcaccg
     5101 tgaagctgtt aataagctcc tggatgatat attcgagcaa tatgtgaatg atctcgcctt
     5161 cgatagagat ttaacccggg atagtgtgat ctcgctgatc gataatgctc ctcatgtttc
     5221 ggaggaagct gtgcaggccg ggctggtcga tacaatacta tatcaggatc aaatcccgga
     5281 ttggcagaaa agccggtttg gaagctcctg ctcgatagat ttcgaagaat actcaaaacg
     5341 aaaaccatac cgcgaaagat ggggcctgcc tccgcagatc gcagtcatcc cggtagaggg
     5401 aacattgatg tcgggttcca gcggcgagat cccgttttat ggaaaaacga caggctccaa
     5461 agatctggcc atggccatag aatatgcccg caaaaacagc cgcatccgcg ccgtagtgct
     5521 gagactgaat acgcccggcg gagacggcct ggcctcggac ctgctccatc gtgaactgga
     5581 actcctgagc aaggagaagc ccctgatagt ctcgatggga aatgtcgccg catccgcggg
     5641 ttatcatctg gcctcggccg gcgattatat ctatgcccag ccttcgactg tcacaggatc
     5701 gatcggagtt gtttatggca agctggacct ctcggacctg cgcgaaaata tcggtttcac
     5761 gacttatcat ctaaaacgcg gaaaaaacgc cgatttcttc tcgtggaata ccggtttcac
     5821 cgagcaacag gagcagaaat tgcagcgcca gctcgatctg atgtacgagg atttcgtgca
     5881 gattgtggcc gagggccgcg atctatccta tgattacgtg gaatctgttg cccaggggcg
     5941 ggtttggagc ggctcagcgg ccagggaatt gaagcttgtg gatgattacg gcggcctctg
     6001 ggatgctctt catgatgcca aagaacgtgc cggaatatcc gatgacgagg tcgaaatcgt
     6061 cagctttcca aaacggccct tccggtttct cgacctctgg agcggtcttt cctcggccgc
     6121 aaagactata aaatcgctaa tgtttggtaa tggcatatta tcagacacgg ccaatgattc
     6181 aggcttttcc agctattatt atattcttcc ctattccatt aaaatctatt gattctactg
     6241 aaacaattgc ttaatttacc cgtctgtaat tgctgaggaa ccaaataact tcttgtaatg
     6301 cttgcccgta attatataag tggaaaaatg cttgaaattg ttttacatat tggattggag
     6361 gaaggatgct gaagaagtca atcctgattg cagcaacagg gctgctattt ctattttctt
     6421 atctggtggc gcaggacaat ccgctgggat ggaagatcac caagacccgg cttgataacg
     6481 gcatgaatat tgtcgtattg gaggatcatt caacaccctc ggtggcctat cagatctggt
     6541 accatgttgg gagcaagaat gagcgtcccg gaatcaccgg aatttcacac atggtcgagc
     6601 atatgatgtt caaaggcacc gatactatcg gcccggaaga acatgcacgt atagtgcagg
     6661 cccggggcgg gacatcgaat gccttcactc atttcgatat gactgcttac catgaggtgc
     6721 tgggcgccca ggacctggaa atcgcggccc agctcgaggc cgacaggatg gccaatcttg
     6781 ctttggattc cgccgaactg gcctcggaaa aagaagttgt ggccgaggag cgcagaacag
     6841 ttatcgataa ttctcccttc gggccattgc tggaacagct cctgaatctc acctacattg
     6901 cgcatcccta tcactggcac gtaatcgggt acatgtcgga catccagaat tatac
//
LOCUS       DSEB01000363            5295 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001156, whole genome shotgun sequence.
ACCESSION   DSEB01000363 DSEB01000000
VERSION     DSEB01000363.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5295)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001156"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..2240)
                     /locus_tag="ENO07_06500"
     CDS             complement(<1..2240)
                     /locus_tag="ENO07_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HDS01653.1"
                     /translation="MSKKASAFLTIFTAVFLLIALTGPASAQNQKDWTIVTSYVIPEA
                     ASGLAWDGTYLYFGIYGSNGSNIYQLDPSDGSYTLLFTGPQEDAYGLTFDGTNLWTTD
                     HAGSSSDPALAIEMDFSGVQQSTITLPDHYMSGIAYDNGDFWVARYYPDPGYLYKVDV
                     AGTILKQFSGPDNQPWDLCMQGEYLWMADYWGDALYKLDTASGTVVETHAAESTDPSG
                     IVWDGTHLWYIDNGGAGGDFLYKVDLYGAGTPFINVSSDSHDYGVVTAGESDTWNLIV
                     SNVGTGNLEVSGVTFSGTGSADLSTTATFPMTIEPDSQKQVPIIWSPLAPGTLDAVAT
                     IASNDPIDPNYDITLTGDAVSSGPDIIVPESAHNYGPVRVGAYTRWFMTIQNGGNDIL
                     TIANISSDDPAFAIESDVTFPIDIPILDEVQVGVWFHPDMDMAYSGSLTISSNDPDEN
                     PYIVSLEGSGSGTEWPIGDTLWQYVITGGTDTSPKAIISIPDITGDGVAEVVVASEDY
                     YIRCFNGNSDGQADVLWEHEIYAGSVYSQKGLDLTDDIDSDGYPDIVVASAWGGKLIR
                     LLSSKDGTEIWTHYTDEYGDGGWVYDVDCSYDYNGDGVRDVLAATGDDANDTGPKRIY
                     CLDVLTGNSIWERPIGAPSFAVMGVEDFTGDSQPDVLVGASNFDESAGRAYGINGATG
                     VIEWTFQAPGSSVWGLAQLDDITGDGIKDVAIGDFSLGGGGVYGLDATTGGQEYGVTG
                     LGSILN"
     gene            complement(2489..3436)
                     /locus_tag="ENO07_06505"
     CDS             complement(2489..3436)
                     /locus_tag="ENO07_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01654.1"
                     /translation="MADFRKIDLGKVKTYSIKKRASKVQLDLLGQPVSGGCCIDDFLK
                     SLPKFLKAEDFKKVVDRMSEAVKRKFKIIFMLGAHNLKVGLTPLYVDFMDIHSNLHFA
                     GNSAVSIHDLEIAFFGATSEDVLENLQDGSFGFVEETAELYSQMLKLAWREEIGLGHA
                     LGRYIADQNPPYKEYSLAYNCLRKNIPLTIHAAIGTDIVNQHPVFDGAAIGRASFEDF
                     QIFADSVMQIANGGVALNIGSAVIMPEVFLKALAVCRNLNPEFGKYTTANFDMINHYR
                     PLQNVVKRPAALNSEGYNITGHHELMIPLLFAAVKSYVE"
     gene            complement(3510..4343)
                     /locus_tag="ENO07_06510"
     CDS             complement(3510..4343)
                     /locus_tag="ENO07_06510"
                     /inference="COORDINATES: protein motif:HMM:PF11306.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HDS01655.1"
                     /translation="MKKIFLTIILALLPAFLSAQYDDPEIEDTLLCSDTLRVPADELE
                     TDNDFRFVRNDAFGVGEKLTFTIGWKMIKAGEATMEVSEVLDHEDRPVYRIRTIAKSF
                     PFFDNFFKVRDTAETWVDVEGLFTWFFRKKLNEGNYENEQVQLYDQSRNIVRAQDTAF
                     VVPEYVQDVLSSLYYVRTLDLEVGDTITISNFEKDKCYPLPVIVHRREIIEVPAGKFK
                     CIKVEPLLQSAGIFKHEGTLSVWLTDDRLKMPVLMKSKVVVGSIYAELTDYRIGELYE
                     W"
     gene            complement(4340..4852)
                     /locus_tag="ENO07_06515"
     CDS             complement(4340..4852)
                     /locus_tag="ENO07_06515"
                     /inference="COORDINATES: protein motif:HMM:PF01451.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HDS01656.1"
                     /translation="MAEDQNNLFRVLFVCTGNTCRSPMAHGILRKMAAEEGLDFMDVE
                     SAGIGTYNGYPPSGYAVQTALRDDVDISDVHSTQLTPELMKEADIVIAMAKNHYDRMR
                     SLYPENFHKVYMLKAFPDQNGDDSLSVADPIGLDAKFYERTYAEIKWELERIWPDIKK
                     RYQNKLGKQI"
     gene            complement(4845..>5295)
                     /locus_tag="ENO07_06520"
     CDS             complement(4845..>5295)
                     /locus_tag="ENO07_06520"
                     /inference="COORDINATES: protein motif:HMM:PF01300.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01657.1"
                     /translation="PTAVFIESEDQLKDYGVELEEKQFSRIGRFWPGPVTFVLKSSIE
                     NWPGVVSADGKIGFRCSSHPFIKSLIGGFKVPISATSANISGKMPASIADIESNFAGK
                     IELFIIDPVLNFNSPPSTVVELVDDDVKILRKGAVDSKLIKEIFAYG"
BASE COUNT         1389 a         1361 c         1181 g         1364 t
ORIGIN      
        1 atatttagta ttgatcccag cccggtaaca ccgtactcct gtccaccggt ggtcgcatcc
       61 agtccataaa caccgccgcc gcctaagctg aaatcaccta tggccacatc tttaataccg
      121 tctccagtaa tatcgtcgag ctgagcgagc ccccatacag atgaacccgg agcctggaac
      181 gtccactcga tcactccagt cgcaccgtta attccatatg ctcttcccgc cgattcatcg
      241 aaattggatg ctccaacaag aacatcgggc tgactatcgc cggtaaagtc ctcaacgccc
      301 atgacggcaa acgagggcgc tccgatgggg cgttcccaga tcgaattgcc ggtcaggaca
      361 tcgaggcagt aaatccgctt cggccctgta tcattagcgt catcaccggt ggccgccagc
      421 acatcacgta caccgtcacc gttatagtca taactgcaat cgacatcata tacccagccg
      481 ccgtcgccgt actcatcagt atagtgggtc cagatttccg tgccatcctt acttgagagc
      541 agacggatca gcttgccgcc ccaggctgac gccacaacaa tatcgggata tccatcacta
      601 tcgatgtcat cagttaaatc cagacctttt tgggagtaaa ccgagccggc ataaatttca
      661 tgttcccaga gaacatcagc ctgcccgtct gagttgccgt taaagcagcg aatgtaatag
      721 tcctcagagg caacgacaac ctcagcaact ccatcacctg tgatatcagg aatggagata
      781 atagccttgg ggcttgtatc tgtaccgccc gtgatgacat attgccatag ggtatcgccg
      841 atcggccact cagtgcccga cccgctgcct tccagggaga ctatgtaagg attctcatcc
      901 gggtcattgc tggagatggt caggctgcct gaatatgcca tatccatatc cggatggaac
      961 catacaccga cttgaacctc atccaggatc ggaatatcaa tcgggaatgt aacatcgctt
     1021 tcgatagcaa aagcagggtc gtcgctcgaa atatttgcaa tagtcagaat atcatttccg
     1081 ccgttttgaa tagtcatgaa ccagcgtgtg tatgccccca cacgcacggg cccatagtta
     1141 tgtgctgatt ccggaacaat aatgtcgggg cctgagctta cggcatctcc tgtcagcgta
     1201 atatcataat taggatcgat cggatcattt gaggcgatcg tagccacagc atccagtgtg
     1261 cccggcgcaa gcggcgacca tatgatcggt acctgtttct ggctgtccgg ctcaatcgtc
     1321 atcgggaatg tggcagtggt ggataaatcc gctgaaccgg tgccggagaa tgtaacaccg
     1381 ctgacctcca ggttgccggt ccccacgttg gagacaatca gattccaggt atccgactct
     1441 ccggctgtta ccaccccata atcgtgggaa tcgctggaga cattgataaa aggcgttccg
     1501 gcgccataca gatcgacctt atagaggaag tctccgcccg ccccgccgtt atcgatatac
     1561 cacagatgtg taccgtccca gacgattccg gagggatcag tagactccgc agcatgggtt
     1621 tccacaactg tcccgctggc ggtatccagc ttatagagtg catcgcccca gtaatccgcc
     1681 atccagagat attctccctg catgcagaga tcccagggct gattatccgg gcccgaaaac
     1741 tgcttcagaa tcgtcccggc gacatcgact ttatagaggt atcccggatc gggatagtag
     1801 cgggccaccc agaaatcgcc attatcataa gcgataccgg acatgtaatg gtccggaaga
     1861 gtaattgtgg actgctggac gccgctaaag tccatctcaa tggccaatgc aggatcggag
     1921 ctgctgcccg catgatcggt tgtccagaga ttcgtaccat caaacgtcaa tccataagca
     1981 tcctcctgcg gaccggtaaa cagcaatgtg tagctgccgt cggatggatc gagttgataa
     2041 atgttactcc cattggaacc gtaaattcca aaatatagat aggtgccatc ccaggccagt
     2101 ccggaggcgg cttccggaat tacataactg gtcacaatcg tccagtcttt ttgattctgc
     2161 gcactcgccg gcccggtcag ggcgattaac aaaaacaccg ccgtaaaaat tgttaaaaat
     2221 gctgatgctt ttttggacat aagactcctc ccgaagttat tttgtaacaa aaaatatcat
     2281 ggttttttac taatcagtat caaatcccga ttttcaggcc atttatattc gagttagtat
     2341 gtgcagtaaa tccggatacg aaaaactaag cgcatgtagt acaacaactt acataatcac
     2401 tgtttggagt ttgccgaata gacaatccat taaaatatag catctaaagc cgaaaagtca
     2461 agtctattaa gttcggcttt aagtttagtt attctacgta acttttaact gcagcgaaca
     2521 ggagcggtat cataagctcg tggtgcccgg tgatattgta tccttccgaa ttcaatgcgg
     2581 cgggccgctt tacaacattt tggaggggac gataatgatt tatcatatcg aagtttgcag
     2641 ttgtatattt tccgaattcg ggatttaaat ttcgacaaac cgccagagcc ttgaggaata
     2701 cctccggcat tataaccgcc gagccaatat tcaaagctac tccgccattg gcgatctgca
     2761 tcaccgaatc tgcgaaaatt tggaaatcct caaaggaggc gcggccgatc gcggccccgt
     2821 caaaaacagg atgctgattt acaatatcgg ttcctattgc ggcatggatg gtcaatggta
     2881 tgttttttct caagcaatta taggccagag aatattcttt gtatggagga ttctggtcgg
     2941 caatatatct gccgagcgca tgacccaggc cgatttcttc tctccaggcc agcttcaaca
     3001 tctgggaata aagttcggca gtctcctcca caaacccgaa actgccgtcc tgaagattct
     3061 ccagcacatc ctctgaggtg gccccaaaga aggcgatctc gagatcatga attgaaaccg
     3121 cactgtttcc cgcaaaatgc agattactgt gaatatccat aaaatcgaca tagagcggag
     3181 tcaggccaac cttgagatta tgcgccccga gcatgaatat gatcttgaat ttccttttta
     3241 cggcttcact catcctatcc acaacttttt tgaaatcttc agctttcagg aatttcggca
     3301 gggatttcag gaaatcatcg atacaacagc cgccggagac cggctggccc aaaaggtcga
     3361 gctgaacctt gctggccctc ttttttatag aatatgtttt gactttaccg agatctattt
     3421 tcctgaaatc agccatggga tttattcctg tatgccaagg ttccgggaga gaataaacag
     3481 gattggggaa ttaaatcaat taaaacttat taccattcgt aaagttcgcc gattctgtaa
     3541 tcggtcagtt cggcataaat cgagcccact accactttcg atttcatgag cacgggcatt
     3601 ttgaggcgat cgtctgtcag ccacaccgag agagtgccct catgcttgaa aatacctgca
     3661 gattgaagaa gaggttcgac tttgatgcat ttgaattttc ctgccggcac ctctattatt
     3721 tccctcctgt ggactatgac tggcagggga tagcatttat ccttctcgaa attagatatg
     3781 gtaattgtat cgccgacttc cagatcaagc gtgcggacat agtaaagcga cgacaaaacg
     3841 tcctgtacat actcgggcac gacaaaggcg gtgtcctgcg ccctcactat atttctcgac
     3901 tgatcataaa gctgaacctg ttcattctca taattcccct cgttcaattt cttgcgaaag
     3961 aaccaggtga acagcccttc cacatcaacc caggtctctg ccgtgtctct gaccttaaaa
     4021 aaattatcga agaatggaaa tgatttggcg attgttcgta ttctatatac cggtcggtct
     4081 tcatgatcga gaacttcaga gacctccatt gtggcctctc cggccttgat catcttccag
     4141 cctatcgtaa aggtaagctt ttcccctacc ccgaaggcat cgtttcttac aaaacggaaa
     4201 tcattatctg tttccagctc atcggccggc acccggaggg tgtccgagca taacagcgta
     4261 tcttcgatct caggatcatc gtattgtgcc gacaggaatg cgggtaaaag agctaaaata
     4321 atagtcagga aaatcttttt catatctgtt ttccaagctt attttggtat cttttcttta
     4381 tatcgggcca gattcgctca agttcccatt ttatttcggc ataagttctc tcgtagaact
     4441 tcgcatccag tccgatcgga tcggccacac tcaatgaatc atcgccattt tgatcaggga
     4501 aagctttgag catatagacc ttatggaaat tttcgggata cagcgaacgc atccggtcgt
     4561 aatggttttt ggccatagca attacaatgt ccgcctcctt catcaattcg ggcgtcaatt
     4621 gcgtggaatg gacatctgaa atatctacat catccctcaa agcggtctga acagcgtagc
     4681 ctgagggagg gtagccgtta taagtgccaa tcccggcgga ttccacatcc ataaaatcca
     4741 atccctcttc cgcagccatt tttcgcagga ttccatgtgc cataggggat cggcaggtat
     4801 taccggtaca gacaaataag acacggaaca gattattttg atcttcagcc ataagcaaaa
     4861 atctctttta tcaattttga atcaacagcg cctttgcgca gtatcttaac gtcatcatca
     4921 accagctcaa cgaccgttga gggcgggcta ttaaaattca ataccggatc aataataaaa
     4981 agttcaattt ttcctgcaaa attactttcg atatctgcaa tagatgcggg cattttgccg
     5041 ctgatatttg cgctggtcgc gctgattgga actttaaatc ccccgatgag ggattttatg
     5101 aaagggtgac tggaacagcg aaaacctatt ttcccgtccg cggagacaac tcccggccag
     5161 ttctcaatag acgatttcag tacgaatgtc accgggccgg gccagaatcg tccgattcgg
     5221 gagaactgct tctcctcgag ttcaacgccg taatccttca gctgatcctc tgattcgata
     5281 aagactgcgg taggc
//
LOCUS       DSEB01000364            3088 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003870, whole genome shotgun sequence.
ACCESSION   DSEB01000364 DSEB01000000
VERSION     DSEB01000364.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003870"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            86..961
                     /locus_tag="ENO07_06525"
     CDS             86..961
                     /locus_tag="ENO07_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01658.1"
                     /translation="MSKIISNPELLDHAAMTRIAQTLALAEWCNGTAVCEIENCYQIS
                     AGSLMSLAETCAWLSESTAAMARVFNLPRNIRLFFKRLAFMLRHGLRFELRSIWKLGH
                     EILGREDYPRLSAQGIRNANDLVSANPDTLEKILGAMKAGKIRKKIQVAKNKNKEDKM
                     NGELACKLVLEGRSNRDRLTVRFFGRELPLTLKSFKYLAKLACAKYLDAQNNGWLHKD
                     QLEPGFNQARYIYNLKKELGLSKDQGLLENNRGGYYRLNLNPDEIRLNLENLKRIQDY
                     ELKSIAERYENLQVS"
     gene            1097..>3088
                     /locus_tag="ENO07_06530"
     CDS             1097..>3088
                     /locus_tag="ENO07_06530"
                     /inference="COORDINATES: protein
                     motif:HMM:PF02190.14,HMM:PF05362.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDS01659.1"
                     /translation="MRKTAEGKAYPVLPLRTTLIYPKCVGTIQINRRRSLNLLLDNKE
                     SSDEFILALLKRPNSSEDSRPDSFCDIVVAAKIIQETKLSSDIISVTFEGLRRMRLGR
                     FVMEEPYFMAYAEDVEETCDVKIPRQAFIEIIKSLKEITLEDNSYSSELLNIAEKSIN
                     DPSYFSDSIAAHLHLPLLTRQRILESLDIPERLTVLKQAIDDELKQNEIQSELDLKVS
                     QSIQRNKKEIYLREQLKQIYKELGEEDPTERLIKHFKDQVAVSAHIPAYVKDRLNLEI
                     ERLKLLSVASAEYGATKNYIELLLELPWSRYTEDETDLKEIEKTIEGEYYGPGRIKER
                     ILEYLVIRKLTAEIRSPILCLAGPPGTGKSSLAAAIARALGRQLININAAGLSIVEEL
                     KGDMRNFPGAVPGRIMRALAEKQSANPVVVIDDLDKMSMYTSNMSLPLAMLEVLDPKQ
                     NKSYIDTYVGIPFDLSKAIFITTVESLEEVPPPLTERMEVIEFTGYIDSEKIEIGQKF
                     IIPKIRKAHKLTEKQLSFTVGALRKIIRNYTLESGLINFKREIELICRKASRMLATQK
                     SRKSVKITERNLESFLGPQVYIPELVETSSEIGIANGLAWTGSGGDIMLIEGIKMRGG
                     GNVIYTGSLGDVMKESIQASHSYVRAKADMLGIDYDDFIN"
BASE COUNT          854 a          715 c          793 g          726 t
ORIGIN      
        1 atcctcgatg cagggcagaa tcaattccgg cgccatttca gagctaatag gtgattttga
       61 gctaaaggaa tatccgcagt tccgattgag caagattatt tcgaatcccg agctcctgga
      121 tcatgcggcc atgaccagaa ttgcacagac attggctttg gcggaatggt gcaatgggac
      181 tgctgtatgt gaaatcgaga actgttatca gatatcagcg ggcagcctca tgagccttgc
      241 ggaaacctgc gcctggctct cagagagcac agcagctatg gcacgcgtgt ttaatctgcc
      301 gcgaaatatc cggctgtttt tcaagaggct tgcgttcatg cttcgccatg gcctgcgctt
      361 tgagctgcgt tcaatctgga agctgggcca cgaaatcctg ggacgtgaag attatcccag
      421 gcttagcgcg caagggataa ggaatgcgaa tgatctggta tccgcaaatc ccgacacact
      481 tgaaaaaatt ttgggtgcga tgaaagcggg gaaaatccgc aagaagattc aggtcgcaaa
      541 aaataaaaat aaggaggaca aaatgaatgg tgaactggca tgtaaactgg ttttggaggg
      601 caggtcaaac cgtgaccgtc tgaccgtcag gttcttcggg cgggaacttc ccctgaccct
      661 caaaagcttt aaatatttag ccaagctggc atgtgcaaaa tacctggatg cgcaaaacaa
      721 cggctggctt cacaaagatc agcttgagcc gggcttcaac caggcgcgtt atatctataa
      781 tctcaagaag gaattgggct taagcaagga tcagggcctc ctggagaata atcgcggcgg
      841 ttattaccgc cttaatctaa atccggatga gattcgactc aatcttgaaa acctcaaacg
      901 gatccaggat tatgagctga agtcgatcgc agaacgatat gaaaatcttc aggttagctg
      961 atggagaatt ctgatgacgg ccggagcggc agataattgt cattttatga aattaatgct
     1021 gttaagatcg tgaacagtac cgatacatgt tataaatggc aatttttcag ttaagtcaaa
     1081 aacatctcga ggtcgtatga gaaaaactgc cgaggggaaa gcatatcccg ttctgcctct
     1141 gcggacaact ctgatctatc ctaaatgtgt cggtaccata cagatcaacc gccgcaggtc
     1201 ccttaatctt ctgctcgaca acaaggaatc ttctgatgag tttattctgg cgctcctgaa
     1261 aaggcccaac agtagcgagg attccagacc cgacagtttc tgcgacatcg tggtcgccgc
     1321 caaaattata caggaaacaa aattatcgtc agatatcatt tcggtcacct ttgagggtct
     1381 gcgccgaatg cgtctcggaa ggttcgtaat ggaggagccg tatttcatgg cctatgccga
     1441 ggatgtggaa gaaacctgtg atgtcaaaat tccgcgccag gcctttatcg aaataattaa
     1501 gtctcttaaa gagattactt tagaggataa ttcgtattca tccgagcttt tgaatattgc
     1561 cgagaaatct atcaatgatc cctcttattt tagcgactct atcgccgccc acctgcacct
     1621 tccgcttctg acccggcaac gcatcctgga atcactcgac attcctgaac ggttgacagt
     1681 tttgaaacag gctatcgatg atgagctcaa acagaatgaa attcaatccg aacttgattt
     1741 gaaagtaagc cagtccattc agcgcaataa aaaggaaatc taccttcggg aacagctcaa
     1801 gcagatatac aaggaactgg gtgaggagga tcccaccgag cgcctgatca aacatttcaa
     1861 ggaccaggtg gcggtctcgg cgcacattcc cgcctatgtt aaggatcgcc tcaatcttga
     1921 gatcgagaga ttgaagcttc tgtcggttgc ctcagccgag tacggtgcaa cgaaaaacta
     1981 tatcgagctt ctgctggagc tgccctggtc caggtacacc gaagatgaaa ctgatttaaa
     2041 agagattgag aagacgattg agggcgaata ttatggtccc gggcggatca aggaacgcat
     2101 ccttgaatat ctggtgatcc gcaaactcac agccgagatc agaagtccaa ttttatgcct
     2161 ggcgggaccg cccggaaccg ggaagagctc gctggcggcg gctattgccc gggccctggg
     2221 acgccagttg atcaatatca acgcggccgg tctttcgatt gtggaagaac tcaagggcga
     2281 catgcgtaat ttcccgggag cggtgccggg aagaatcatg cgcgcactgg cggaaaaaca
     2341 gtctgccaat ccggttgtgg ttattgacga cttggacaag atgtccatgt ataccagcaa
     2401 catgtccctg cccctggcca tgctggaggt tctcgacccc aagcagaata agtcctatat
     2461 cgatacctat gtgggaattc ccttcgatct ctccaaagcg atctttatca ccaccgtgga
     2521 atcgctggaa gaggtgcctc cgcccctgac cgagaggatg gaggtgattg agtttacggg
     2581 ctatattgac tcggagaaaa tcgagatcgg tcagaaattt atcatcccca agatccgcaa
     2641 agcccacaag ctgacagaga aacaattgag cttcacggtt ggggcgcttc gtaaaatcat
     2701 ccggaattat accctggagt cgggactgat caatttcaag cgtgaaatcg aattgatctg
     2761 ccgcaaagca tcgcgtatgc tggcaactca aaagagcagg aagagcgtaa agatcaccga
     2821 aagaaatctg gagtcatttt tggggccgca ggtatatatt ccagagcttg tcgaaacttc
     2881 ttccgaaatc gggattgcca acggactggc atggacgggt tccggaggcg atatcatgct
     2941 catagagggc atcaaaatgc gcggcggcgg gaatgtcatt tacaccggct ctctcgggga
     3001 tgtaatgaag gaatccatcc aggcctcgca ttcttatgtg cgcgcgaaag cggatatgtt
     3061 ggggatcgac tatgatgatt ttataaac
//
LOCUS       DSEB01000365            3087 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003872, whole genome shotgun sequence.
ACCESSION   DSEB01000365 DSEB01000000
VERSION     DSEB01000365.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003872"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..992)
                     /locus_tag="ENO07_06535"
     CDS             complement(<1..992)
                     /locus_tag="ENO07_06535"
                     /inference="COORDINATES: protein motif:HMM:PF04389.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent exopeptidase M28"
                     /protein_id="HDS01660.1"
                     /translation="MIKATISIGSRNALFIGLLLAALLFAGNMAIGEEEAVRGDLYKI
                     VVSSKTDAVNLESSPAVPVLALHDGYLAIVEPGDQEILDESGLNYTLLASNVSRSELA
                     LDNRMDRENAEKFEIVYESGDMRVFRVDFEQIEKREPRPQLIPLRTARVPIVYPEAEK
                     FDRDMISGVGDLLTLISIVSQDSLESYTYRLQAFYRRLAGTDSNYAARDWIQSKFIEF
                     GYDSVYLDPFTSYVGNCYNVVAVKEGSIRPDLQVVVGGHFDGVSGSPAADDNGSGTAA
                     TLELARILMNEETDVTFIFITFDSEEQGLNGSYHYADNAAANGDSIIYMFNMDM"
     gene            1333..2340
                     /gene="egtD"
                     /locus_tag="ENO07_06540"
     CDS             1333..2340
                     /gene="egtD"
                     /locus_tag="ENO07_06540"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HDS01661.1"
                     /translation="MRIISPGDSVSTFGDLEAFALDVLVGLSEMNKRLPSKYLYDAEG
                     SRLFTEITRLKEYYPTQCEKETLEKNKDRIVSQIGDEPFNLIEFGSGDGQKTSLLLDH
                     FLKMDLDFQYVPIDISRSAMIELCDELDRRFPLIQVEAIVSEYFSGIKWLNNRSERKN
                     LVLFLGSNIGNFSHAEARVFLRNLWSCLNSGDLVLIGFDLKKDIELLLRAYNDCEGVT
                     AKFNLNMLARINRELGGQFDLSKFRHFGTYDVFSGAMESYLVSLEKQEVFIEEIGRSF
                     SFGPWEPIHVEYSYKYLQSDIDNLARETGFVVREHLHDSKKYFIDSIWEVKKSENNTS
                     E"
     gene            2350..>3087
                     /locus_tag="ENO07_06545"
     CDS             2350..>3087
                     /locus_tag="ENO07_06545"
                     /inference="COORDINATES: protein motif:HMM:PF07992.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HDS01662.1"
                     /translation="MSKHSGHYDAIIIGTGQGAVPLAVDLAGAGRRIAVIEKNHLGGS
                     CINFGCTPTKTMVASARVAYLAGRGSDYGVETGEIKIDFKKIRGRKREIVKSFRSGIE
                     KRLDHDNINLFRGEAGFISEKKIRIRKQHEEQHITGDQIFINTGTRPAIPPVEGIDDI
                     GYLDSESIMELDSQPEHLLIIGGGYVGCEFGQMFRRFGSKVAIIQLGAQLLGREDSDV
                     SEEVAKIFEQDGIEVFLKTGAKSVIRKN"
BASE COUNT          894 a          706 c          721 g          766 t
ORIGIN      
        1 atcatgtcca tgttgaacat ataaataata ctgtcgccgt tggcagcggc attgtcggca
       61 taatgataag aaccattaag tccctgttct tcagagtcga atgtgataaa gataaaggtg
      121 acatcggtct cttcattcat caaaattcgt gcaagttcca gtgtagcggc tgtgccggaa
      181 ccattatcat cagcagccgg cgaaccgctc acgccgtcaa aatgaccgcc caccacaacc
      241 tggagatcgg gccggataga gccctccttg accgcaacaa cattatagca attaccgaca
      301 taggatgtaa aaggatcaag ataaaccgaa tcgtaaccga attcgatgaa cttggattga
      361 atccagtccc gggccgcata gttggaatcg gtgcccgcca ggcggcggta gaaagcctgc
      421 aggcgatatg tatatgattc cagcgaatcc tggctgacaa tcgaaatcaa tgtcagcaaa
      481 tcacctacac cggaaatcat atcccgatcg aatttttcag cttcaggata gacaatcggc
      541 actcttgcag ttctgagcgg aatgagttga ggccgcggtt ccctcttttc aatctgctcg
      601 aagtccaccc ggaacacccg catatctccg gattcatata ctatctcgaa tttttcggca
      661 ttttccctgt ccatacgatt atccagtgcc aattcagaac ggcttacatt agaagcaaga
      721 agagtgtagt ttaaaccgga ttcgtccaga atctcctgat cgccaggctc tactatcgcc
      781 agataaccat catgcaaagc cagcaccggc acagccggag agctctctaa atttacggca
      841 tccgtttttg atgaaacaac tatcttatat aagtcgcccc ttaccgcctc ttcttcgcca
      901 atcgccatat ttccggcaaa taaaagcgct gccaataata aaccgataaa taatgcattt
      961 cttgatccaa tgctgattgt ggccttaatc attctacctc caggactctt atgatatgta
     1021 aaagaagatt ttttatccaa gctcttgtaa gaaatacgaa aagtatgact tcaatgctgt
     1081 ttcttgtttc aaaataccat ctttacaaat attgtcaacg attttagagt caagttcttc
     1141 aattgcaata aaataatcct cataaagcca tagtcggaac ataattctga tcctgcggtt
     1201 ttagctaata aataggacca actttataat atttataata gctcatgtcc agagcctaaa
     1261 acccgggagc cgtcagggaa cttttcctga ttgatatcaa ccgatatgaa aaccaatcat
     1321 aagaatcgac agatgagaat tataagcccc ggcgatagtg taagtacttt cggggatctg
     1381 gaagcttttg cgcttgacgt gctggtgggg ctttcagaga tgaacaaaag actgccctcg
     1441 aaatatcttt atgatgccga aggcagccgg ctgtttacag agataaccag gctcaaggaa
     1501 tattatccaa cccaatgcga gaaagaaacc ctggagaaaa ataaagaccg gattgtcagt
     1561 cagattggtg atgaaccatt taatttaatc gagttcggct cgggggatgg ccagaagacc
     1621 agcttgcttc tggatcattt cctgaaaatg gatctggatt ttcaatatgt gccgatcgat
     1681 atctcgaggt ccgcaatgat cgagctgtgc gatgaacttg accgccgatt tcccttgata
     1741 caggtggaag ctatagtctc ggaatatttt tcgggtatca aatggctgaa taatcgttca
     1801 gaaaggaaaa acctggtgct ctttctgggt tcgaacatcg gcaatttcag ccatgccgag
     1861 gcgcgtgtat tcctccgcaa cctctggagc tgcctgaaca gcggtgacct cgtcttaatc
     1921 ggcttcgatc tcaagaagga tatcgagctg ctgctcaggg cttacaacga ttgcgaggga
     1981 gtgaccgcga aattcaacct caatatgctg gccagaatca accgcgagct gggcgggcag
     2041 tttgacctgt ccaaattcag gcatttcggc acatatgatg tcttcagcgg agctatggaa
     2101 agttatctgg tcagtctcga gaagcaggaa gtctttatag aagaaattgg aagatctttc
     2161 agcttcggac cctgggagcc gattcatgtg gagtattctt ataaatactt gcaatccgat
     2221 attgacaatc tcgccagaga aaccggcttc gttgtccgtg aacacctgca tgattccaaa
     2281 aaatatttca tcgattccat ctgggaagta aaaaagagcg agaacaatac ttccgaatag
     2341 gggcaacgct tgagcaaaca ttccgggcat tacgatgcca taataattgg caccggccag
     2401 ggagcagtac ccctggcagt cgaccttgcc ggggccggac ggagaatagc cgtaatagaa
     2461 aaaaaccatc tgggcggaag ctgtatcaac tttggatgta caccgactaa aactatggtt
     2521 gccagcgcac gggtggcgta cctggcagga cgcggatcgg actacggcgt ggagacaggg
     2581 gagattaaaa ttgatttcaa gaagattcgt ggacgaaagc gcgaaatcgt aaaaagcttc
     2641 cgaagcggta tcgaaaaacg ccttgaccac gataatataa atctgtttcg aggtgaggca
     2701 ggattcatat ctgaaaaaaa aatcagaatc cgaaagcagc atgaggaaca gcacattacc
     2761 ggagatcaga tttttatcaa taccggtaca cgtcccgcaa ttccccctgt tgagggtatc
     2821 gatgatatcg gctatctcga ttccgaatcg attatggagc tcgattcaca gcctgagcat
     2881 cttttaatca tcgggggcgg atatgtcggc tgtgaatttg ggcagatgtt caggcgtttt
     2941 gggagcaaag tcgccattat ccagcttggc gcgcagcttc tgggacggga ggattccgac
     3001 gtctccgagg aagtggcaaa aatatttgag caggacggca tcgaagtctt cctcaaaacc
     3061 ggggcgaaaa gcgtcatcag gaagaat
//
LOCUS       DSEB01000366            2016 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1009896, whole genome shotgun sequence.
ACCESSION   DSEB01000366 DSEB01000000
VERSION     DSEB01000366.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1009896"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..453
                     /locus_tag="ENO07_06550"
     CDS             <1..453
                     /locus_tag="ENO07_06550"
                     /inference="COORDINATES: protein motif:HMM:PF05343.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28"
                     /protein_id="HDS01663.1"
                     /translation="TVQEEVGLRGAGTAAYMGDPDVGIVTDVTVARDTPGMEGEPPEK
                     MGSGPQIVVYDGSMIPNVKLRDLVIDTAEKNKIPYGLSYLERGGTDGGRIHMTKFGVP
                     SIFIGLATRYIHGHASMIYRDDYLNCVKLITAVVKRLDKKTVQSLTQV"
     gene            475..1318
                     /locus_tag="ENO07_06555"
                     /pseudo
     CDS             475..1318
                     /locus_tag="ENO07_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001634482.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
     gene            1707..>2016
                     /locus_tag="ENO07_06560"
     CDS             1707..>2016
                     /locus_tag="ENO07_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01664.1"
                     /translation="MQIETHAIGLGLLFIDEGETFSDNLGIIVFSPGDLDSDGYSEVF
                     ASNALGEIKVYYGGNPADTLPDKQYLGHAGRYTWLSDINGDAYNDFSMLRNESGIEYI
                     D"
BASE COUNT          494 a          444 c          555 g          523 t
ORIGIN      
        1 actgtacagg aagaagtcgg tctgcgtggt gccggaactg ccgcatatat gggcgatccg
       61 gatgtcggca tcgtgaccga tgttaccgtc gctcgcgata cccccggtat ggagggcgag
      121 ccgccggaga agatgggttc ggggccgcag attgtggttt atgacggcag catgataccg
      181 aatgtaaaac ttcgtgatct ggttatagat acggccgaga agaacaagat tccctatggc
      241 ctcagctacc tggaacgcgg cggcaccgat ggtggacgga ttcatatgac caaattcggt
      301 gtgccctcga tatttatcgg cctggccaca cgctacattc atggccacgc ttccatgatc
      361 taccgtgatg attatctgaa ctgcgtcaaa ctaattacag cagtcgtgaa acgtttggat
      421 aagaaaacgg tacaatcgtt aacacaggtg taactcaaaa cgaaaggatt tgtgatgcga
      481 ataaagatca acctgtttct ttttgcatta gttttcgcca cattttttgt cttttccggt
      541 cttctggctc aggaggagag tccgataatt caggaagatg ttcctgaggt tgagggcgat
      601 accataacga ccgcatcgaa cctgaaatat atcgacgttg aactgggtga gggcaaaaaa
      661 gctgaagaat gggattcggt aaaagtccat tataccggat ggctgacatc cggcaagaaa
      721 tttgacagct cacgggatag gggcgagcct tttacagtcg tagtcggcga gcatagagta
      781 attccaggat gggaagaggg cctgcagggc atggctcccg gcggcaagcg catgctgata
      841 attccgcctg acctgggtta cggcaaccgt gctatggggc cgattccggc caattcaaca
      901 ttgatcttcg aggtcgaggc cttagaggtc agcgagggcc ccaagccgcc caaaccgccg
      961 ctgtatgatg aaaaggatgt gaaagagacc gattccggtc tgaaatatgt tgtgctggag
     1021 aagggcaacg gcgaacagcc cgaaaaaggt cagacagtcc aggtgcacta ttaccggatg
     1081 gcttctggat ggagccctgt ttgacagttc caaaaaccgc ggccagccgc tggaatttgt
     1141 gctgggtgcg ggccgtgtaa tcaagggctg ggatgagggt gtggccatga tgaatgtcgg
     1201 cggcaagcgc ctcctgatta tcccgcccga gctcggctac ggttcccgcg gagcaggcaa
     1261 tgttatcccg cccaacgcga cactgctctt tgaagtggag ttagttggaa taaagtaagt
     1321 aatttaatcg aaatatttct catataaagg gccggttttg ccggcctttt gtatttgcag
     1381 atcaatgtgc gctgcccaaa attatttttt cgtgcgcgga cctgtccggc ccctgacgga
     1441 gtacgtcctc gttctccgcg tccgctgggc gcaccgggag atgctctcaa agtgaattat
     1501 cattggcaga atcattttat tccggattct gccctaccgg gtggcacgcc caaatttatt
     1561 tgggcgtgat tttcattgac gtcagattcc aattaattat cttgagattg aaccacgtag
     1621 ccggatgctg aacattgaat tctatattct gtacttcggg gatcttgcaa agagcatcga
     1681 tagtaatttt tgtttcttgt ttttttatgc agattgaaac ccatgctata ggtctgggcc
     1741 ttctttttat agatgagggt gagacttttt cggataattt ggggattata gtattctccc
     1801 ccggcgatct ggattcggat ggctattcgg aagtttttgc ctcgaacgcg ctgggagaaa
     1861 tcaaggtata ctatggggga aatcctgcag atactcttcc ggataagcaa tatctgggtc
     1921 atgctggaag atatacctgg ctatcagata taaatgggga cgcttataat gatttttcaa
     1981 tgcttcggaa tgaatccggg atcgaatata ttgata
//
LOCUS       DSEB01000367            7306 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000582, whole genome shotgun sequence.
ACCESSION   DSEB01000367 DSEB01000000
VERSION     DSEB01000367.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7306)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7306)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7306
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000582"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            89..1840
                     /locus_tag="ENO07_06565"
     CDS             89..1840
                     /locus_tag="ENO07_06565"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00733.19,HMM:PF13537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01665.1"
                     /translation="MPGLFGIVEFEKRPDGSLFNNLASKITPYKNAYLQSEAENCLLG
                     FKSLAIVDPKGQPFYDRQNSLALFFWGEVFERDNKTPRIPEYIFELYLEDRLEELKEI
                     NGFFSLALWDDRNRILILATDIHGSRPLYCYPNKNSIIFAPNPFALAGTLGLSKVNRD
                     AMLQFLTFSKMFYNHTWIGGIMKLRYGQILRLDKSELRTTQYFFPCYDPVKMSIDDAA
                     DGILERLEKAVIRMSEGKAALSLSGGGDSRLLAALLKKNKILIPAFTFGGNESEDIRI
                     AEEVCAHLNMPHHKLLISQDFLEHHLEEAVYNTGGYTSALNFHGISTRYEVKKICDVC
                     ISGLYGNNYLGYISWFLYKLYQNNRSGGLKDIHDKFFAKGQVLDSLERLTAFAIDRSE
                     LINGISTILDIYTFQTMLATWMMLDHFEINSQSSLAGFWLENDLLEFRSPYGDRDLLE
                     FNFKIPVEYKLMMNLGRHIWSKYFPGLGEIDHQRTGLPINSSLKKLIIQKVASRVFKI
                     RPRAGIMEYSAVFEHNLKDWLKDFLLSEKARIREYLNMDTVAIRVGTHTESDTENASQ
                     IGLLLTLEQVLRILEPD"
     gene            1950..2354
                     /locus_tag="ENO07_06570"
     CDS             1950..2354
                     /locus_tag="ENO07_06570"
                     /inference="COORDINATES: protein motif:HMM:PF02579.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01666.1"
                     /translation="MEVPMDTKKIAIATDDKETISQHLGRANYFAIYTITPDEVIDRQ
                     YIKNEAAHHRKHRSHDDHKRVHREMISQLSGCQVILAAGMGDPIRKDFEEAGMKGYVV
                     VEGGSIEEAVQQYLDGELMINRDGGCEHNRRE"
     gene            complement(2356..3375)
                     /gene="lpxD"
                     /locus_tag="ENO07_06575"
     CDS             complement(2356..3375)
                     /gene="lpxD"
                     /locus_tag="ENO07_06575"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:PRK00892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HDS01667.1"
                     /translation="MQISTAELAKKLGAELVGDAQLMLYGLAPVKDAQEGELAFLSNH
                     KYFKFLESTKASAVIVPPEIRESKKTLLIHPQPYFAFSQALKLFYPQQKKYSDGVNDR
                     AITGDNCSIHTSVHIGAYAVLEDNVSIGENTKILPGAFIGSDSKIGKDCLIYPNVTIR
                     EGTRIGDRVIIHSGTTIGSDGFGYVQHEGKSHKIPQVGVVVVEDDVEIGANCAIDRAT
                     LGETRIGKGTKIDNLVQIAHNVKMGENCIIISQVGISGSTKLGDNVILAGQAGLIGHL
                     NIGNDVIVAAQSGVAHDLEDKTRVLGSPAHDMMHQKRIEAILNRLPDYVKRIREIEKR
                     LKDLE"
     gene            complement(3381..3920)
                     /locus_tag="ENO07_06580"
     CDS             complement(3381..3920)
                     /locus_tag="ENO07_06580"
                     /inference="COORDINATES: protein motif:HMM:PF03938.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HDS01668.1"
                     /translation="MNIMKNLLWTVITAFLVSLVFTNPGLAQSGKIVYVDSYRIRSEY
                     SEFLEAQEQFDKELDDWQQEAMEMEKEVDSLKKDLSEKALLLSEEARAKRQEVIDAKE
                     AALQKYMVDVFGQSGKAEQRNRELTRPLLDKITAVLERIAQEQNYDYVFDAVNGNIAY
                     AKKSLDITDKVLEELQKMQ"
     gene            complement(3950..6238)
                     /gene="bamA"
                     /locus_tag="ENO07_06585"
     CDS             complement(3950..6238)
                     /gene="bamA"
                     /locus_tag="ENO07_06585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HDS01669.1"
                     /translation="MKFSRLLFSLTLLLTVLFAAGLSAQNLTISSIEVEGNKLADKSL
                     ILSVSDFTIGSVLTSTATQDAVRQIYELGLFSDVQLLGEIQDGKYIDLIIKVEEYPVV
                     TEVQYKGNDEISDKDLREIDSIRIGQMISPNQVLSSVKQIKAKYQEKGYYRVKIESEL
                     KEVTDDPLTNEHVLVFEIDEGPKIRIREIEFVGNEIFTDDKLRGKMGNKPKGFLRSGN
                     FDPEKHEEDKEKIVRFYREEGYIDAAVLSDSVIIDENDPRWMTVQIEVYEGPRYYFGE
                     TSFYGHKVYGENALYDQLTYEEYDVYNPEKFEESIGQLYSLYQEQGYIHARIFENLTT
                     VDSMINVEFEISEGVPAKINKIEIVGNTRTKEKVIRREMFSRPGQIFRRSLLERSVRN
                     VMLLNYFESAIPDFRVLPNGDVDLVMEMKEKPTGQFNAGAGYSGQDGLVGTVSLGMPN
                     FRGNGQNLSLAVEFGANRNSVSISFSEPWLFGTPTSFSSNIYNLNRRWYDDFWEGRRG
                     GSIRFGRRLSWPDDYFRLYGGYRLEDVRYYDFEDTTSYPDLQALNEDWLRTSSVNMSI
                     VRDSRDLPLFPTRGSVYRFTTEYAGGPLGGYYEFHRHTFEASKFIPLFWKFVLAGKMK
                     VGVVDSPDGEDGVPFTERFSPGGTDPDGTIRGYDDGRVTPKNSLGQSIRGRAMLVYNA
                     ELQVPIVEQQIYALLFADAGNSWLAGYQISPFNKDELYKSYGAGFRIVVPGVGVIGFD
                     FGYGINADDPGWKPHFQVGTTF"
     gene            complement(6256..6570)
                     /gene="rplU"
                     /locus_tag="ENO07_06590"
     CDS             complement(6256..6570)
                     /gene="rplU"
                     /locus_tag="ENO07_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004834209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDS01670.1"
                     /translation="MYAVFETGGLQFEVAEGDSLNIPRLPISPGDTVKFDRILLIKGD
                     SDPLVGKPYIENAAVEAEVVNQGKAEKIIVSTYKRRTKYRRKAGHRQPYTEIKINKIS
                     VP"
     gene            6765..>7306
                     /locus_tag="ENO07_06595"
     CDS             6765..>7306
                     /locus_tag="ENO07_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HDS01671.1"
                     /translation="MAEFKLVSTYKPTGDQPRAIEELADGLKRNRRFQTLLGVTGSGK
                     TFTMANVIQKYGRPTLIISHNKTLAAQLYGEFKAYFPNNAVEFFISYYDYYQPEAYIP
                     VTDTYIEKDTSINDDIDRLRLRATASLLQREDVIIIASVSCIYGIGSPQNYKEQMAFV
                     KKGETRDRDELLRDLISIHYS"
BASE COUNT         1907 a         1814 c         1631 g         1954 t
ORIGIN      
        1 cgtctttgac gatattgtga ctttgcgagg attgaagaat aattaaatta ttgattgacc
       61 ccgcatcgaa agcggggttt tttacaatat gcccggttta tttggaatag tcgaatttga
      121 gaagagacct gatggatcgt tgttcaataa tctcgcctca aagatcacac cctacaaaaa
      181 cgcctatctc caatcggaag ccgaaaactg ccttctgggt ttcaagtcgc tggcgatagt
      241 cgatccgaaa ggccagcctt tttatgatcg ccaaaactct cttgctctct tcttctgggg
      301 agaggtgttt gagagggata ataagacgcc tcgaataccg gaatatatat ttgaattata
      361 tctggaagat cgcttggaag agctgaaaga gataaacggc tttttcagtt tggccctctg
      421 ggatgacagg aacaggatat tgatacttgc gactgacatt catggcagcc gtcccctgta
      481 ctgctatcca aataagaact cgatcatctt tgcccccaat ccgtttgcat tggccggaac
      541 tctgggttta agcaaagtga atcgtgatgc tatgctgcag tttttgacct tttcaaagat
      601 gttttataat cacacctgga ttggcggcat aatgaaactg agatatggtc aaattctgcg
      661 cctggacaaa tcggaacttc gtacgaccca atatttcttt ccctgttatg acccggtaaa
      721 aatgagcatc gatgatgccg cggacggaat actggaaaga ctggaaaaag cggtaatccg
      781 gatgtctgaa ggaaaggcgg ccctgtcgct gtcaggcgga ggcgattcac ggcttctggc
      841 ggcgctgctt aagaaaaata aaatcctgat acccgccttc acatttggag gcaatgaaag
      901 cgaggacatc aggattgctg aagaagtatg cgctcatctt aatatgcccc accataagct
      961 attgatctcc caggatttcc tcgagcatca tctcgaggaa gcggtttaca ataccggcgg
     1021 atatacttca gctttgaatt tccacggtat atcgaccagg tatgaggtta aaaagatttg
     1081 cgatgtatgt atttcgggat tgtatgggaa caattacctc gggtacatct cctggtttct
     1141 gtacaaacta tatcaaaaca accgatcggg gggactcaaa gatattcatg ataaattctt
     1201 tgccaagggg caggtgcttg acagcctgga acggctgaca gcatttgcta ttgaccgcag
     1261 cgagttaata aatggaatct ctacaattct tgacatttat actttccaaa ctatgctggc
     1321 cacatggatg atgttagatc attttgaaat taattcgcaa agctcgctgg ccggattctg
     1381 gctcgaaaat gacctgctcg aatttcgcag cccttatggt gaccgggacc tcctggaatt
     1441 taatttcaag attccggttg aatataaatt gatgatgaac ctcggcaggc atatctggag
     1501 caaatatttc cccggcctgg gtgagattga tcaccagcgt accggcctgc ccataaattc
     1561 atcgttaaaa aaactgatta tccagaaggt agcctcgcgt gtattcaaga taaggcctcg
     1621 cgccggaata atggaatatt ctgcggtatt tgagcataat ttaaaggatt ggctgaagga
     1681 tttcctattg tcagaaaaag cccgcattcg tgagtatctt aatatggaca cggttgccat
     1741 aagggtcggc acccataccg aatctgacac tgaaaacgcg tcacaaattg gattattgct
     1801 gaccctcgag caggtgctca ggatacttga accggattaa tatttagtac tctgataatg
     1861 atagagcgga aacaaccatt aagatagata ttttcatctt tatttttcga taagtgtgta
     1921 catctcgtgt aattgagcgt ataatgtaaa tggaggtgcc gatggacacg aagaagattg
     1981 caattgctac agacgataaa gagaccattt cgcaacatct ggggcgcgcc aattatttcg
     2041 cgatatatac gattaccccg gatgaggtaa tcgaccgtca atatataaaa aatgaagctg
     2101 cccatcatag aaaacaccgt tctcatgatg accataagag agtgcatcgc gagatgatca
     2161 gtcagctttc cggctgccag gtcattctgg cggcaggcat gggagatcct atccgcaagg
     2221 actttgaaga agccggtatg aagggttatg ttgtggttga aggggggtcc atagaggaag
     2281 ctgtacaaca gtatctagat ggtgagctga tgataaatcg cgacggcggt tgcgagcata
     2341 accgtcgtga atagactatt ccagatcctt caagcgtttt tcgatttcac gaatcctttt
     2401 tacataatcc gggaggcggt tcagaatcgc ttcaatccgc ttttgatgca tcatgtcgtg
     2461 ggcaggcgat ccgagaaccc tggttttatc ttccaggtca tgagcgactc ctgattgcgc
     2521 cgcaacgata acatcattac ctatattaag atgtcctatc agcccggcct ggccggccag
     2581 gatcacatta tcacccagct ttgtcgagcc ggaaattcca acctgtgaaa ttatgatgca
     2641 gttttcaccc atcttaacgt tatgcgcgat ctgcaccagg ttgtcgatct tggtaccttt
     2701 accgattcgt gtttcaccca gcgttgcgcg gtcaattgcg cagttggctc cgatttcgac
     2761 atcatcttct actaccacaa caccgacctg cggaattttg tggcttttac cttcatgctg
     2821 cacatatccg aatccatccg aaccgattgt ggtgccgctg tgaattatca ccctgtcacc
     2881 aattcgagtt ccctcccgta tcgtcacatt gggatagatg aggcagtctt tgccgatctt
     2941 gctgtcactg ccgataaacg ctccgggcag gatttttgta ttttcgccga tactgacgtt
     3001 atcctccaga acggcatacg cacctatatg gacgctggtg tgaatggagc agttatcgcc
     3061 ggtgatcgca cggtcgttca ctccgtcgga gtattttttc tgctgcgggt agaatagctt
     3121 tagcgcctgt gaaaaagcaa agtagggctg gggatggatc aggagcgtct ttttcgactc
     3181 cctgatctcg ggaggcacaa tgactgcgct ggctttcgta ctttccagga acttaaaata
     3241 cttgtgattg gaaagaaagg ccagttcccc ctcctgtgca tccttaacag gggccagccc
     3301 ataaagcata agctgggcat ctccgacaag ctcggcgccc agcttcttcg ctaattcagc
     3361 agtactgatc tgcatgccgg ttattgcatc ttctggagtt cttccaggac cttatcggtg
     3421 atatcgaggc ttttcttggc ataggcgata ttgccgttca ccgcatcgaa tacataatcg
     3481 taattctgct cctgggcaat tctttccagc acagcggtta ttttatcgag aagcggtctg
     3541 gtgagttccc gattgcgctg ttccgccttt ccgctctgcc cgaaaacatc gaccatgtat
     3601 ttctgcaatg ccgcttcctt ggcatcgatc acttcctgcc ttttggcacg ggcttcttcc
     3661 gagagcagaa gcgccttctc gctgagatcc ttcttgaggc tgtccacttc cttctccatc
     3721 tccatagctt cctgctgcca atcatcaagt tccttatcga attgctcctg ggcctccagg
     3781 aattcgctgt attcgcttcg gatgcggtag gaatcgacat atacaatctt gccgctttgt
     3841 gccaaacccg gatttgtgaa aaccaggctc acaagaaaag ccgtaatcac tgtccataag
     3901 aggtttttca taatattcat cacttcccct atcttaaatt aactatcatc taaaaagttg
     3961 tgccaacctg gaagtggggc ttccagcctg gatcatctgc attaatacca taaccaaaat
     4021 caaaaccgat cacacctacg ccgggcacca caattctgaa tccggcgcca tatgacttat
     4081 acaattcatc tttattaaaa ggcgaaattt gatatcccgc cagccaggaa tttccggcat
     4141 cggcgaaaag taaagcataa atctgctgct ccacgatcgg cacctgaagc tcagcattgt
     4201 aaaccagcat cgcccgtcca cgaatgctct gccccaggga attcttggga gtcactcgac
     4261 catcatcata cccccggata gtaccgtcgg gatcggttcc gccgggagaa aatctctctg
     4321 taaacggcac accatcttcg ccgtccgggg agtcaaccac gccgaccttc atcttgcccg
     4381 ccagcacaaa cttccagaat aacggaataa atttggaggc ctcaaaagtg tgtctatgaa
     4441 attcgtaata gccgcccaat ggcccgcccg cgtattcggt ggtaaatcta tataccgagc
     4501 ccctggtcgg aaagagcggc agatccctgg aatcacgcac aatcgacata ttgaccgatg
     4561 atgtgcgcag ccagtcttca ttcaatgcct gcaaatcggg atagctggtt gtatcttcga
     4621 agtcataata gcggacatcc tcgagcctgt aaccgccgta aagacggaaa taatcatcag
     4681 gccaactcag ccgccttccg aatctaatcg atccgccccg ccggccttcc cagaaatcat
     4741 cgtaccaccg acgattcaaa ttgtaaatat tgctggaaaa tgaagtcggc gtcccgaaca
     4801 gccacggctc ggaaaaactg atcgaaacgg aatttctgtt ggccccgaat tcaaccgcca
     4861 gggaaagatt ctgcccgtta ccgcggaaat tgggcatacc cagggaaaca gtacccacca
     4921 agccgtcctg cccggaataa ccggcacccg cattaaactg gccggtcggc ttctccttca
     4981 tctccataac caggtccaca tcaccgtttg gcagaaccct gaaatccggt atagcgctct
     5041 caaaataatt caggagcatg acattacgta ccgaacgttc cagaagcgaa cggcggaaaa
     5101 tctgtccggg acgcgaaaac atctcccgcc gaattacctt ctctttggtc ctggtattgc
     5161 cgacaatttc aatcttgtta attttggccg ggacaccttc gctgatctcg aattcaacat
     5221 ttatcatcga atcgacagtc gtgagatttt cgaagatgcg ggcatgtatg tagccctgtt
     5281 cttgataaag ggaatatagc tgaccaatgg actcttcaaa cttttcggga ttataaacat
     5341 catattcctc ataagtcaac tgatcgtaaa gcgcattttc tccgtaaacc ttgtgaccat
     5401 agaagctggt ttcgccgaag taatagcggg ggccctcata gacttcgatt tgaaccgtca
     5461 tccatcgtgg atcattttcg tcaattatga cagagtccga taaaactgcc gcgtctatat
     5521 agccctcctc gcgatagaac cggactatct tttccttatc ttcctcgtgt ttttcaggat
     5581 caaaatttcc tgagcgcaga aatcccttgg gcttgttacc cattttaccg cgcaatttat
     5641 cgtcagtgaa aatctcgttt ccgacaaatt cgatctcccg gattctgatt tttggccctt
     5701 catcgatttc aaaaaccagc acatgctcat tggtaagcgg atcatcggta acttccttca
     5761 gctccgattc aatctttacc ctgtagtagc ccttttcctg atatttcgcc ttgatctgtt
     5821 taacagagga gaggacctgg ttgggcgata tcatctgccc tatcctgatg gagtctattt
     5881 ccctcagatc tttatcgctt atctcatcat tgcccttgta ttgaacctca gttacgacag
     5941 ggtattcctc caccttaatg atcaggtcga tatacttgcc gtcctggatt tcccccagca
     6001 actggacatc ggaaaagagc cccagttcat agatctgcct gaccgcatcc tgggtggcag
     6061 tagaggtcaa aaccgaccct atggtaaaat cggaaacgga caggatcagg ctcttgtcag
     6121 ccagcttatt tccttcgacc tcaatcgatg agatcgtcag gttctgggcc gacaggcctg
     6181 ccgcaaaaag aactgttaag agaagagtga gtgaaaataa aagcctgctg aatttcatcc
     6241 ttcttgaacc ctgaatcacg gtacggatat tttgtttatt ttgatttccg tatagggttg
     6301 acgatggccg gctttgcggc gatatttagt gcgtctcttg taagtcgaaa ctattatttt
     6361 ttcagcttta ccttgattaa cgacctcggc ttccaccgcc gcattttcaa tgtatggttt
     6421 tccaaccaac gggtcgctgt cgcctttgat aaggagaatc ctgtcaaatt tgaccgtatc
     6481 tcccggggat atgggaagcc tgggaatatt gaggctgtca ccctcggcca cttcaaactg
     6541 caacccacca gtttcaaaaa ctgcgtacat caagtctcct ttcgagattc caaaaaacta
     6601 agccggaatt atacgaaaat caaaccttat gtcaaggcca ttttgcattc tcaatgtttt
     6661 aaacatcagt gcctataaat gttccagata tatgaaactg acaggcttgc caagcgttta
     6721 aatcatcttg accgggtgca ataatttcag cataatagag tatcatggca gaattcaaac
     6781 tggtgtcgac atacaagccc acaggcgatc agccccgggc aatcgaggaa ctggcagacg
     6841 ggctgaagag aaatcggcgt ttccagacac tcctgggtgt aaccggttcc gggaagactt
     6901 tcacgatggc caacgtgatc cagaagtacg ggcggcccac tctcattatc tcccataaca
     6961 aaactctggc ggcccagctt tacggcgaat tcaaagcata ctttcccaac aatgcggtgg
     7021 aattctttat ctcctactac gattactatc aacccgaagc ctatatccct gtgacggata
     7081 catatattga aaaagacacc tccataaacg atgatatcga tcggctcagg cttcgggcta
     7141 cagcgtcctt gttgcagcgt gaggatgtca tcattatcgc atcggtttcc tgcatttacg
     7201 gaataggatc gccccaaaac tacaaagagc agatggcttt cgtaaaaaag ggagagaccc
     7261 gtgatcgcga tgaactcctg cgcgatctga tcagcatcca ttattc
//
LOCUS       DSEB01000368            4728 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001495, whole genome shotgun sequence.
ACCESSION   DSEB01000368 DSEB01000000
VERSION     DSEB01000368.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001495"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(173..1603)
                     /locus_tag="ENO07_06600"
     CDS             complement(173..1603)
                     /locus_tag="ENO07_06600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDS01672.1"
                     /translation="MRLTPGTDARIKKIDLTEGNILKNIFRMGIPSAIGFFSVNLYDF
                     IDALWVAQLGASHVAAITIFFGFYWVISSLNQLAGTGSVSVISQNYGNNDHDRAEAAI
                     KETYLLKWGIAIFFGTLGLIFLEDVMRLLGASTETIGENGDSVLSLAIDYGTIQILGL
                     GFSMCAFTNYTALRGVGNPKMAMWIMIASVCFNIILDPFLIFGWWIFPYMGIKGAALA
                     SVISHILAFFAGLAIFYGGFANVKLNLKGKIPIRLKTILKIIRIGAPSGLNSVSFSLS
                     RSIILAFVAMTTTEAVAAYGIGNKISALSIMIIVGLGLGLAALIGQLLGAEKPERARK
                     TAYQAMAIALVITISIALLTIIFAEQIMKIFFDPSIGSSEAAVVAIGVKMLRIISISL
                     PFVGLYIIMEMTFTGAGNNVPPMVFGILTGWVLEIPLVYGALRVLDAGPVGVWWALTI
                     SSIIGISACFWWFRKGHWINVRVKGD"
     gene            complement(1684..2757)
                     /gene="rtcA"
                     /locus_tag="ENO07_06605"
     CDS             complement(1684..2757)
                     /gene="rtcA"
                     /locus_tag="ENO07_06605"
                     /inference="COORDINATES: protein motif:HMM:PRK04204.1-5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-phosphate cyclase"
                     /protein_id="HDS01673.1"
                     /translation="MKNNSMVRIDGSYGEGGGQILRTSLMLATHLGRPLEIVNIRKNR
                     KTPGLMPQHLTAVRALAEICSAEVKGDDHRSEKLYFKAGKVRTGKYTFDIAAEKRSAG
                     SAPLVLQTLALPLFLAQGGGSTLKVKGGTHVPWSPPGTYLKQIFFPMLAKMGLYAHIK
                     ILKWGFYPEGGGEISLQIKRATNSRNIKCFNFTDRGKLLRVTGLSAVSNLPMSICERQ
                     RRRANEILTEAGIKPNIAAKEARARGRGTVLFLTAEYENVLAGFSRLGEKGKPAEIIA
                     EETCQDLIDFNGKNVGIGHNLADQLLPYLCLTGRKFTLEVSSLTTHLLTNLWVISRFL
                     DVTIDIKGAQGLPGTITVIPKSG"
     gene            complement(2844..3563)
                     /locus_tag="ENO07_06610"
     CDS             complement(2844..3563)
                     /locus_tag="ENO07_06610"
                     /inference="COORDINATES: protein motif:HMM:PF13489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDS01674.1"
                     /translation="MLNFNRSNEIEIMDSGDYTDEELRGDFKNIRLANKLTRTYSNLK
                     SILNDIINKKNLEDITILDVGTGMADIPLELANHCSENGTRAKIKGIDPNPRAIKIAR
                     EFIKDHRDIEFEARSIQDFRDDEKFDFVISNHTLHHIPDADILETLNKMFRMARHGLI
                     ISDIRRTRLGHCGASIISKFIGNRLTSNDTPASIRKALSKSELIQVLQESDIENYRIE
                     YSFPYKFILVAEKGQNSTKSF"
     gene            complement(3697..4347)
                     /locus_tag="ENO07_06615"
     CDS             complement(3697..4347)
                     /locus_tag="ENO07_06615"
                     /inference="COORDINATES: protein motif:HMM:PF07589.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HDS01675.1"
                     /translation="MGLYAMKKLLATTVILLLISTSAFAQFTFHGYLQTPDGVQGTVP
                     WSENFRIEWWVTQQMDNSWLYQYNLTLIDGSPILDHAVSHWYVEVSPNVTAADFWGFE
                     GGWEFVDETHGGTPFPNALKLDYGDADADGYSFYSWRAPVWGDFYAKDGVDDEGQVYA
                     WNTGFYDPDPLDAAADGSIGYKILRPDTTTVIPEPTTMLLLGMGMLGLAARKLRRK"
BASE COUNT         1194 a         1153 c         1172 g         1209 t
ORIGIN      
        1 gcattaatat tcagtcgatc tcgacttctc tttcctccat aacagtagtg gtgagagtgg
       61 atcagatcga tgaagccgaa gcatgcttga aggaagaatt cggcatatag aaataggggg
      121 tcagagacga tagattctgc ctttggtttt ttatgtccct ctatggataa aactaatctc
      181 ccttgactct cacatttatc cagtgcccct tacggaacca ccagaagcag gccgaaatgc
      241 cgataattga ggaaatggtg agcgcccacc agacaccgac aggcccagcg tccagaactc
      301 gaagagcgcc gtaaaccagc gggatctcca ggacccatcc agtcaggatt ccgaaaacca
      361 tggggggaac attatttcct gcgcctgtga aagtcatctc cattattata tataatccca
      421 cgaagggcag gctgatagaa atgatcctga gcattttgac cccgattgcg acaacagcgg
      481 cctcggacga accgatggat gggtcgaaga atattttcat gatctgttcg gcaaaaataa
      541 tcgtgagaag cgcgattgaa attgtaatga ccaacgctat tgccatcgcc tgataagcgg
      601 tcttgcgggc acgttccggc ttctccgcgc ccaggagctg gcctatcaga gccgccagcc
      661 ccaggcccag gccgactatt atcatgatgc tcaaggccga gattttgttg ccgataccat
      721 aggctgccac cgcctcggtg gtggtcatcg ccacgaaggc taagattatt gaacgtgaga
      781 ggctgaatga taccgaattg agcccggaag gcgccccgat ccttatgatt ttcaggatcg
      841 tcttcaagcg tatgggtatc ttgcctttaa gattcaattt gacgttggca aagccgccgt
      901 aaaaaatggc caatcctgcg aagaatgcca gtatgtgaga tatgaccgag gccagcgccg
      961 cacccttgat acccatgtac ggaaatatcc accatccgaa aatcaggaat ggatcgagga
     1021 taatattaaa acagaccgaa gcaatcatta tccacatggc catcttggga ttgcccactc
     1081 ctctcagggc ggtgtaattc gtaaatgcgc acattgagaa gcccaatccc agaatctgaa
     1141 tagtaccgta atctattgcc aggctcagta ccgaatcgcc gttttcgccg atagtctcgg
     1201 tggatgcgcc cagcagccgc atgacatcct ccaggaatat caaccccagg gtaccgaaaa
     1261 atatcgcaat tccccatttg agaagatacg tttccttaat tgcggcttcc gcccggtcat
     1321 ggtcattatt cccgtaattc tgggagatca ctgataccga cccggtgcca gccagctgat
     1381 taagagaaga gataacccag tagaatccga agaagatggt gatggcagcg acatgcgagg
     1441 cccccagttg agcaacccag agggcatcga taaagtcgta gagatttacc gagaagaatc
     1501 cgatggccga gggaatcccc atgcggaaaa tattctttag aatattgcct tcggtcaggt
     1561 caatcttttt tatccgcgcg tctgttccgg gtgttaatcg catatgccat ctcaaccatt
     1621 agaaaatata ttacggcgca agttcaggat ggttttgcgg cttgggcctg atttataaag
     1681 aatttagcct gatttcggga ttaccgtaat agtgccggga aggccctggg ccccttttat
     1741 gtcgatggta acatcaagaa aacggctgat tacccagaga tttgtcagca ggtgggttgt
     1801 caggctggag acctccagcg taaacttcct gcctgtcagg caaagatagg gaagaagctg
     1861 gtcggccaga ttatggccga tgcccacatt tttgccattg aaatcgatca aatcctgaca
     1921 tgtttcctcg gcgatgattt cagccggctt gcccttttct cccagccgcg aaaaaccggc
     1981 cagaacattt tcatattcag cggtcagaaa caaaactgtg ccacggcctc gcgcccgggc
     2041 ctctttggcg gcaatgttgg gtttgatacc cgcttcggtc aggatttcat tcgcacggcg
     2101 cctctggcgt tcgcaaattg acatgggcag gttcgagaca gccgaaagtc cggttacgcg
     2161 aagcagtttt cccctgtcgg taaaattgaa acattttata tttctgctgt tggtggccct
     2221 tttaatctgc aggctgatct cgccgccccc ctccgggtag aagccccatt tgaggatttt
     2281 tatatgcgca taaagcccca ttttggccag catgggaaag aaaatctgtt tcaggtatgt
     2341 ccccggaggg gaccagggga catgagttcc gcccttgact ttgagcgtgg agccgccccc
     2401 ctgtgccaga aaaagcggca gggccagggt ttgcaggacc agaggggcag agccggccga
     2461 tctcttctcg gcggcgatat cgaatgtata tttgccggtc ctgacttttc cggccttgaa
     2521 atacagcttc tctgagcgat ggtcgtcccc cttgacctcg gccgagcaaa tctccgccag
     2581 cgcccgaact gctgtcaggt gctggggcat gagtccggga gttttgcggt ttttacggat
     2641 atttacgatt tccagcggac ggcccagatg ggtggccagc atcaggctgg tgcgcagtat
     2701 ctgtccgccc ccctcgccat aggaaccgtc aattctgacc atactgttgt ttttcataag
     2761 gccataattt taacactgtt agatgtaaaa agcaagcgaa ataaaggcgg gaatgaattg
     2821 cagatcagat cgtaataatt gatttaaaaa gattttgtac tattctggcc tttttccgct
     2881 accagaataa acttgtaggg aaagctatat tcaatgcgat aattctcgat atcggattcc
     2941 tgcagaacct gaattagttc agattttgag agggcttttc tgatcgaggc gggggtgtcg
     3001 ttggaggtca gacggttgcc tatgaatttt gagatgatac ttgcgccgca atggcccagc
     3061 ctggtcctgc ggatatcgct gatgatcagg ccgtgacggg ccatgcgaaa catcttatta
     3121 agtgtctcca gaatatcagc atcggggata tgatgaaggg tatgattgct gatcacaaaa
     3181 tcgaacttct catcatccct gaagtcctgg attgatctgg cttcgaattc gatatcccta
     3241 tggtctttga taaactccct ggcaattttt atcgctcgcg gattggggtc gatacccttg
     3301 attttcgcgc gtgtgccatt ttcgctgcag tgatttgcaa gttccagggg gatatcggcc
     3361 attccggtac cgacatcgag aatagtaata tcctccagat tcttcttatt gattatgtca
     3421 tttagaatac tcttcaggtt gctgtatgtg cgggttagtt tattggccag acgaatattt
     3481 ttgaaatctc cccggagctc ctcgtcggta taatctcccg agtccatgat ttcaatttcg
     3541 tttgacctgt taaaattcag catgcaataa accggcaagt taacttaaca tattgattgt
     3601 tgcttataac agggatggta tgtcgggtgt tgcactataa ataaaaaaag cgccgcctga
     3661 cggcagcgct ttttaatttt gaataccgat tctgtgttat ttcctgcgca gtttccgggc
     3721 tgcgagaccg agcattccca taccaaggag aagcatcgtg gtcggctccg gaattacagt
     3781 agtcgtatcc ggacgcagaa tcttgtagcc gatggaccca tccgctgcag catctaacgg
     3841 atcgggatca tagaatcccg tgttccaagc ataaacctgg ccttcgtcat cgacaccatc
     3901 ttttgcatag aaatcgcccc agaccggcgc tctccatgag tagaatgaat aaccatctgc
     3961 atcggcatca ccataatcga gtttcaaagc attcgggaac ggtgttccgc cgtgagtctc
     4021 atcgacgaat tcccagccgc cctcaaaacc ccagaagtcc gccgctgtga cgttagggga
     4081 aacctcaacg taccagtgac tgacagcgtg gtctaagatt ggactaccat cgatcagagt
     4141 caggttgtac tggtataacc aggaattgtc catctgctgt gtgacccacc attcgatcct
     4201 gaagttttcg ctccagggta cagttccctg aacaccatca ggagtctgca ggtaaccatg
     4261 gaaggtaaac tgtgcaaatg ccgatgtgga gatcagaagc agtattactg ttgtggcaag
     4321 tagctttttc atagcataaa gccccatcta agagttaaac gtttattttc atatttcagg
     4381 gaaatataga gcagattgcg tgccgaataa agcccgggat gcgcatcctt aagctattgg
     4441 tatcattgga tttagagaag cggtttagaa gccgatgcgc ggggattgcc cccgattgat
     4501 cggaattccg acaccccaaa gtggagtttt ttataagtta tgggtattaa tgaggttata
     4561 tcgatccggg aaatgtcgga aatctggaca aaatatcgac tttttgcagt aaaatagcgg
     4621 cctgaaggca tgccgccggt cgacaaaagc cctcaatcaa aggccgaaga tctgatccgc
     4681 tttggccagt ccttccatca gccgatccac atcttccctg gtgttgta
//
LOCUS       DSEB01000369            2161 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1008537, whole genome shotgun sequence.
ACCESSION   DSEB01000369 DSEB01000000
VERSION     DSEB01000369.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1008537"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..413)
                     /locus_tag="ENO07_06620"
     CDS             complement(<1..413)
                     /locus_tag="ENO07_06620"
                     /inference="COORDINATES: protein motif:HMM:PF12679.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01676.1"
                     /translation="MELRSINLISKHKFAKLLRSKIYWVFFIVGLGMLLMTIIPFLVA
                     EDFRNESGPMLLIIISTFTKSYTFFGVFASVTIGSLVLIQDIRDGTIFPYLAKPVSRS
                     AFIFGKIIGAFKLMIIFWIFQVIYFMILLYTVTDYG"
     gene            complement(376..1338)
                     /locus_tag="ENO07_06625"
     CDS             complement(376..1338)
                     /locus_tag="ENO07_06625"
                     /inference="COORDINATES: protein motif:HMM:PF00005.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDS01677.1"
                     /translation="MNGNGENAIEIQNLEMVYSDLWGHARVRALDGISFEVKKGEIFG
                     FLGPNGAGKTTTIHILMNFIYPTHGKAMIHGLPVNNSKCREKVGFLPELFEFDKFLKG
                     KKLLKYMGNLAGFSSNEVERHGRDLLEFFEMAEAEGRKVKTYSKGMKQKIGLAQAMIS
                     DPDLLILDEPTSGMDPIAKSKIKHKFLELKKRGKTVFLSTHILSDVQQIADRVAIINK
                     GKVIKIDTVENLLRKSDDTEVTFTLDKPKAEQLRETWKPENKGDSAYSILTGSKEDKA
                     RLIAKLSELGADIISVIPPRTDLEEIFMQLVENGTEIDKSNQQA"
     gene            complement(1339..1971)
                     /locus_tag="ENO07_06630"
     CDS             complement(1339..1971)
                     /locus_tag="ENO07_06630"
                     /inference="COORDINATES: protein motif:HMM:PF13098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDS01678.1"
                     /translation="MTRKSFYITFLILAVIFVIQHVLIKQPDILKKQAAAENFSDIEI
                     NLLSGSFEPIGYYQDKKPVVLVFWNSNSNPSHLAIENIGRKIDEWNEEYDFELIAVNV
                     GESPEEVVNVKELWNINLKIGLDPDRMIAEEFGVGQLPTVIIIDDKGEIQKRWDGMQE
                     DIEYGIQRRLGDDEDDVSVKIKKNNHGETTEVEIKSGDSIISVDTIRKKD"
BASE COUNT          582 a          509 c          405 g          665 t
ORIGIN      
        1 ccatagtccg taacagtgta gaggagaatc ataaagtata ttacctggaa aatccagaag
       61 ataatcatca gcttgaatgc gccaatgatt tttccaaaga taaatgcgct gcgcgatacc
      121 ggtttggcaa gataggggaa aattgttcca tcccgaatat cctgaataag tacaagggaa
      181 ccgattgtca ccgaagcaaa cactccaaag aaggtgtaac ttttagtgaa tgtcgaaatg
      241 atgatcagca gcatgggacc gctctcattg cgaaagtcct cggcaaccag aaacgggatt
      301 attgtcatca ggagcattcc aaggcccact ataaagaaga cccagtagat tttactcctt
      361 agcaattttg caaatttatg cttgctgatt agatttatcg atctcagttc cattttccac
      421 caattgcata aatatttctt ccagatcggt gcggggcggg ataaccgaaa ttatatccgc
      481 accgagttcg gaaagctttg ctattaatct ggccttatcc tctttagacc cggtcaatat
      541 cgaataagcc gagtcgcctt tattttcggg cttccaagtt tccctgagct gttctgcttt
      601 gggcttatct agtgtaaaag tcacttcggt atcatcagat tttctaagca gattttcgac
      661 cgtatctatc ttgataacct tacccttgtt gatgatcgcc acacgatcgg cgatctgctg
      721 aacatccgac agtatgtgtg tactcagaaa gactgttttt ccgcgctttt tcaattccag
      781 gaatttgtgc ttgatttttg atttggctat aggatccata cccgaggtcg gttcatcgag
      841 tatcagaaga tccggatcgg aaatcattgc ctgtgcaagg ccaattttct gcttcatacc
      901 cttggaatag gtttttacct tacggccctc ggcctccgcc atctcaaaaa actccaaaag
      961 gtcccgtcca tgcctttcaa cttcgttcga ggaaaacccg gcgagattgc ccatatattt
     1021 cagaagcttt ttgcccttca ggaatttatc gaattcaaac agctccggaa ggaatcctac
     1081 cttttcacgg catttggaat tattaacagg gagtccatgt atcatcgcct taccatgagt
     1141 ggggtaaatg aaattcatga gaatatgaat ggtcgttgtt ttgccggcgc cattggggcc
     1201 aaggaagccg aagatctcac cttttttgac ctcgaacgat atgccatcca gagcgcgcac
     1261 ccttgcatgc ccccacagat cggagtaaac catctcgagg ttctggattt ctatcgcatt
     1321 ctcgccatta ccattcatct aatctttttt ccttatagta tccacgctaa taatgctatc
     1381 acctgattta atttctacct cggtagtctc cccatgatta tttttcttta tcttcaccga
     1441 aacatcgtcc tcgtcatctc cgagccttct ctggattccg tactcgatat cttcctgcat
     1501 gccgtcccaa cgtttttgaa tctctccctt atcatcgata ataatgacag tgggcagctg
     1561 accaaccccg aattcttcag ctatcattct atccggatcg agccctattt tcaaattgat
     1621 attccatagc tcttttacat tcacaacttc ttccggagat tcaccaacat ttacagcgat
     1681 caactcaaaa tcatattctt cattccattc atcaattttg cggccaatat tctcgatggc
     1741 cagatgcgat ggattcgaat ttgaattcca gaataccaat actaccggct ttttgtcctg
     1801 ataataaccg ataggttcga agctgccact taataaattg atctcaatat cggaaaaatt
     1861 ctcggcagcg gcctgctttt tcagtatgtc cggttgttta ataagtacat gctgaataac
     1921 gaatatcact gccaatataa gaaatgttat gtagaaggac ttacgggtca taattaatct
     1981 gctgaaattt cactgaatat attatttgtt tacgggaatt caacagattt gttataattt
     2041 tccataaaaa tcaaaatctt attcaagagg cgaaacatag ttgttccagc gttcttgctg
     2101 gattcccgat ggcgatttca tcatgcttac ctgatatatt tcgctgcctt ccaggacaag
     2161 c
//
LOCUS       DSEB01000370            2322 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1007275, whole genome shotgun sequence.
ACCESSION   DSEB01000370 DSEB01000000
VERSION     DSEB01000370.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1007275"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(81..>2322)
                     /locus_tag="ENO07_06635"
     CDS             complement(81..>2322)
                     /locus_tag="ENO07_06635"
                     /inference="COORDINATES: protein
                     motif:HMM:TIGR03936.1,HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HDS01679.1"
                     /translation="FNSATELNYTNILSMIDLAGIPLLAKDRADSDPLVAIGGGCAFN
                     PEPLAQFADFFFLGDAEEGILKILEVLKSGSDKPREEKLRALAQIQGVYVPSFYRDQY
                     AEDGKFRSLTPTEESIPHKIKARVVRELKNSYYPATPMLPAVETVHDRLAVEIMRGCG
                     HACRFCQATVIYKPARKRSVDDIVSQVTTSLEKSGYDEVTLLSLSSGDYPEIELLVRR
                     LVDKLKDRHVAVSLPSLRISGLSLELAKLITENKRTGLTFAPEAGTERLRQVMNKPVD
                     EDTLVEVLTEAFKQGWQTIKLYFMVGLPTETEEDLRGIADLITRLNRISEKYRGRRSF
                     NITISPFSPKAHTPWQWEKQIGIEETYQKIDFLKRTIRKRNVHLKFHDPRTTWLEGLL
                     GRGDRRIGDVILRAYRMGAHMDGWSENFDFETWQEACREERIDPDRYLAERSTGSPLP
                     WDHIEKGLSKESLLKELAKSRGLTDLVKSLDKEEKPEKEPPERKRKDDNERIMYGRAP
                     KVQKAQAAGIVPQSRLRIKWGRSGLSRFLSHLDNMKAMERALRRANLPISFSHGFRPK
                     PKISYGPPLSLGFTSEAEYFDIQLDVPVHDYMLGRLSREFPPDFSLLGTKPLLGKTQS
                     LASQLNLAVYEVYLPMDIEKARQKCAEILEAEELHFQRETKSGPVEVEGRKAIIDLAC
                     EKSDDGKTRLTMKTGMADLGFIKPLELLEHGFEISSEVLPALEIHRKALYRLENGNLI
                     DPFDLI"
BASE COUNT          523 a          625 c          553 g          621 t
ORIGIN      
        1 agttgcataa ttctaaccat tatacccctg agacaaaaat atttcaacta ttttaatttt
       61 tgcggaaaaa ttaatcggca tcagattaag tcgaaggggt ctatcagatt gccattctcc
      121 agacgataca gggcttttcg gtgaatttcc aaagcgggca ggacctcgga tgaaatctca
      181 aatccatgct cgaggagctc caggggcttt ataaagccca gatccgccat accggtcttc
      241 atggtcaacc tggtcttgcc atcatccgat ttctcgcaag ccagatcgat tatcgctttg
      301 cgtccctcaa cctcaacggg gcctgattta gtttcacgct ggaaatgaag ttcctcggct
      361 tcaagtatct ccgcgcattt ctgtcgagcc ttttctatat ccatgggcaa ataaacctca
      421 tacaccgcca ggttcaattg tgaggcaagc gattgtgtct tacccaggag tggtttcgta
      481 ccgagcagac tgaaatcagg ggggaactcc ctgctcaagc ggccgagcat gtagtcatga
      541 accgggacat ccagctgtat gtcaaagtac tcggcttctg aagtgaatcc cagcgacagg
      601 ggcgggccat agctgatttt tggtttgggc ctgaagccgt ggctgaagct gattggcaga
      661 ttggcccttc ggagagcacg ttccattgcc ttcatattat ccagatgcga gaggaatcgc
      721 gacagtccgc tccgtcccca tttaatccgc aggcggctct ggggtactat acctgcagcc
      781 tgcgcttttt ggacttttgg cgcgcgccca tacataatcc tctcattatc gtcttttctt
      841 ttcctctcag gcggttcttt ttcaggcttc tcttctttat ccagcgattt gaccaagtca
      901 gtaagacccc gcgattttgc cagttctttc aacagagact cttttgacaa acctttttca
      961 atatgatccc aggggagagg acttccggtg gacctttcag ccagatatct gtcgggatcg
     1021 attcgctcct cccggcaagc ctcctgccag gtttcgaaat caaaattttc gctccagccg
     1081 tccatatgcg cacccatccg ataggccctg agaataacat caccaattct gcgatcgccg
     1141 cgtcccagca gaccttccag ccaggtggtc ctgggatcgt gaaatttcag atgcacattg
     1201 cgcttcctga ttgttctctt caggaaatct attttttggt aggtctcctc gataccgatc
     1261 tgtttttccc attgccaggg agtatgcgcc ttgggtgaga aaggggagat ggtgatattg
     1321 aagcttctac gtcccctgta cttttcggaa atccggttga gtctggttat caaatccgct
     1381 atcccccgta gatcttcctc tgtttctgtg ggcagtccga ccataaagta gagtttgata
     1441 gtctgccagc cctgcttgaa cgcctctgtc agaacctcca ccagagtatc ctcatcgacc
     1501 ggcttattca tcacctgccg cagacgttct gttccggcct ccggggcgaa agtcaggccg
     1561 gtccgtttgt tctcagttat cagcttcgcc agttcgaggg acaatcccga aatacgcagc
     1621 gacggcagag agactgccac atgcctgtct tttaatttgt caaccagtct tctgaccagc
     1681 aattcaattt caggataatc gccggaggat aacgacagca gggtcacctc gtcataaccg
     1741 cttttctcca ggctggtggt aacctgggat actatgtcat caaccgaacg cttgcgggca
     1801 ggcttgtaga ttacggttgc ctggcagaaa cggcaggcat gcccgcagcc acgcataatt
     1861 tccaccgcca ggcgatcgtg tacagtttcg actgcaggaa gcatgggagt tgcagggtaa
     1921 tatgaattct tcagctcgcg gacaacgcga gcttttattt tgtgaggtat ggactcctct
     1981 gtcggagtca acgacctgaa ctttccatcc tccgcatatt gatctctata aaatgagggc
     2041 acatagacac cctgtatctg cgccagagct ctgagttttt cctcgcgcgg cttatcgctt
     2101 ccggacttta aaacctccag tattttcaga atcccctctt ctgcgtcacc gaggaagaag
     2161 aaatcggcaa actgggccaa tggttcggga ttaaaggcgc atcctccgcc tatcgccacc
     2221 aggggatcac tatcggcgcg gtccttcgcc agcaggggaa taccggccag atcgatcatc
     2281 gagagtatat tggtataatt caattcagtg gccgagttga ag
//
LOCUS       DSEB01000371            1914 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1011150, whole genome shotgun sequence.
ACCESSION   DSEB01000371 DSEB01000000
VERSION     DSEB01000371.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1011150"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..279
                     /locus_tag="ENO07_06640"
     CDS             <1..279
                     /locus_tag="ENO07_06640"
                     /inference="COORDINATES: protein motif:HMM:PF01790.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01680.1"
                     /translation="QLYMSFYGLALFLVLHYLDRKDHFSGMLFAVFLIVEAFFRFVIE
                     FVRYYEEAMIPDLFGMELTYNHLVAVILFFLGWFLLFHLKGRSKQAQS"
     gene            complement(280..624)
                     /locus_tag="ENO07_06645"
     CDS             complement(280..624)
                     /locus_tag="ENO07_06645"
                     /inference="COORDINATES: protein motif:HMM:PF14478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HDS01681.1"
                     /translation="MEKGRFNKMKLKATVLIIFALIVVLSCTGKNQEDGEVHTVILEG
                     RDGRTVFDILIDKHLVDYIEADKGIFIESIDGIENAGGHYWTYSINGKPGRVACDKAF
                     VSDGDTIRWEYK"
     gene            complement(597..965)
                     /locus_tag="ENO07_06650"
     CDS             complement(597..965)
                     /locus_tag="ENO07_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDS01682.1"
                     /translation="MSPIKYFTQIRLQSDSRSLRHFMVQYAERYGIKPAARMFNTTPK
                     TVKKWLRRKDNGSDDWLVDQRSLSKPRKSRIPEKEKQRVIELKKRHRSWGAMRIKREY
                     GLAISDKAMRKIWRKEGLTK"
     gene            1091..1708
                     /locus_tag="ENO07_06655"
     CDS             1091..1708
                     /locus_tag="ENO07_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDS01683.1"
                     /translation="MDKKRRQAYELHSQVAGRFCVRIGLTPNLITFTSLIMSVVAAIV
                     LWKGWFIWGIVFIILTSITDMLDGATARAGNMGTTFGGILDHVLDRYAEFFILSGILL
                     SGKVHPVWPIFAIFGMIMASYSRKAAESIGKIPSAEVGWVGRLEKFIVIMIGLALVWF
                     FPEFDPAGLNLLEIALIFVGVVSHITAIQRLFYAKKELDRRAQNG"
     gene            1701..>1914
                     /locus_tag="ENO07_06660"
     CDS             1701..>1914
                     /locus_tag="ENO07_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDS01684.1"
                     /translation="MANIFSIGNPVFDIIKTPHVQTDGRVLSGCSTNFCLAMARMDVR
                     TTLVGNVGPDFRSAFESEMKKNGIDYI"
BASE COUNT          445 a          456 c          450 g          563 t
ORIGIN      
        1 cagttataca tgtcctttta tggtttggcg ctcttcctgg tgctacatta tctagacagg
       61 aaagatcatt tcagcggaat gctgtttgca gtattcctga ttgtggaagc ctttttcagg
      121 tttgtaatag aatttgtcag gtattacgag gaggccatga tacccgatct tttcggcatg
      181 gagctgacct acaatcacct ggtggctgtg attctgtttt ttctgggatg gtttttgctc
      241 tttcatctca agggccgctc gaagcaggct caaagctgat tatttgtact cccacctgat
      301 agtgtcgcca tccgaaacaa aagccttatc acaggcgacc cggcccggtt taccattgat
      361 agaatatgtc cagtaatgcc ctcctgcatt ctctatacca tcgattgact cgatgaaaat
      421 tcccttatcg gcttcaatgt agtcaaccag atgcttgtca atcaagatat cgaaaacagt
      481 cctgccgtcc cggccttcga gaattacagt atgtacttcg ccatcttcct gatttttccc
      541 ggtgcaggaa agaactacga ttaaggcgaa tattatgaga acagtggctt ttagtttcat
      601 tttgttaaac cttcctttct ccatattttt cgcatggctt tgtctgatat cgccaggcca
      661 tattcccttt tgatcctcat tgctccccag gatcgatgtc gctttttcaa ttcgatcacc
      721 ctttgttttt ccttctccgg aatacgggat tttcgcggtt tcgataatga acgttggtcc
      781 accaaccagt catccgaacc gttgtccttc cgccggagcc atttcttcac ggttttcggc
      841 gtagtattga acatccgcgc agccggcttg atgccgtatc tttccgcata ctggaccatg
      901 aagtggcgca gcgaacgcga atcgctctgc aggcggattt gggtgaaata ttttatcggg
      961 ctcatagagt tatagcctta gctaacaggg atactatata agttccgtac cgttttataa
     1021 tataaatata cttgcaaaaa aattccagta atttatatta gcctctttga aatgagataa
     1081 aggaaaattc atggataaga agagacggca ggcttatgaa ctgcattccc aggtggccgg
     1141 aaggttctgt gtcaggatcg gcctgacccc caacctgata acattcacca gcctgataat
     1201 gtcggttgtc gccgctattg tgctctggaa aggctggttt atctggggca tcgtattcat
     1261 aattctgacc tcgattactg atatgctcga cggcgccacc gcgcgtgcag gcaatatggg
     1321 aacgaccttt ggcggaatcc tcgaccatgt gctggaccgt tacgcggaat tctttatcct
     1381 ttccgggata ttgctttcag gaaaggtcca tccggtctgg ccgatcttcg ctatctttgg
     1441 aatgattatg gcctcctact cgcgcaaggc agccgagtcg atcggcaaga ttccctcagc
     1501 cgaggtgggc tgggtaggaa ggctggagaa attcatcgtt atcatgatcg gcctggcgct
     1561 ggtatggttt ttcccggagt tcgacccggc cggactgaac ctgctcgaga tagctctgat
     1621 tttcgtcgga gtcgtttcac atataactgc aatacaaaga cttttttacg ccaagaagga
     1681 attggacagg agggcacaaa atggctaaca tcttctctat cggtaatcct gtattcgaca
     1741 taataaagac tccgcatgtg cagactgacg gccgggtgct gtccggctgc tccaccaatt
     1801 tctgtctggc tatggccagg atggatgtgc gcacgaccct ggttggaaac gtgggcccgg
     1861 attttcgttc agcctttgaa tccgagatga aaaagaatgg catcgattat ataa
//
LOCUS       DSEB01000372            7386 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000571, whole genome shotgun sequence.
ACCESSION   DSEB01000372 DSEB01000000
VERSION     DSEB01000372.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7386)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7386)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7386
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000571"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..592)
                     /locus_tag="ENO07_06665"
     CDS             complement(<1..592)
                     /locus_tag="ENO07_06665"
                     /inference="COORDINATES: protein motif:HMM:PF16653.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase"
                     /protein_id="HDS01685.1"
                     /translation="MQIALLGSGMMGKAIAYDLAQNDKIDKILVCDIDLSNAEEVAAF
                     AKSDKVVPTWLDVRNPSGTKNTISGCSVIISAIHYQFNLQLSRLAIEIGANFIDLGGN
                     NRVVGEQLKLHEQAKSKGVTIIPDTGLAPGLVQILTSSGSSRFDELENVHLRVGGLPQ
                     NPEPPLNYKIVFSVDGLINEYIEDAVVIRDGKVQNVK"
     gene            complement(671..1333)
                     /locus_tag="ENO07_06670"
     CDS             complement(671..1333)
                     /locus_tag="ENO07_06670"
                     /inference="COORDINATES: protein motif:HMM:PF01471.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HDS01686.1"
                     /translation="MPDSEERHKLHVCRDGDWMGRIARDFNHADPAPIYDHDKNSYLR
                     EKRPERNLLARGDEVWVPVLPVPEFPTRGKNGGAVSISVTRAVRERITTRLKNTEGES
                     LTNLDYTLRHPEQDEEVSASTGGEGQVDQELPPGVESLSMELEEKVMTWNMGYMDPPR
                     TVSGYAYRLKNLGYFNHDIPEKKNMELYRALRRFQEDNDLSPTGNADTPTINKLIELH
                     GC"
     gene            complement(1454..4786)
                     /locus_tag="ENO07_06675"
     CDS             complement(1454..4786)
                     /locus_tag="ENO07_06675"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00801.18,HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HDS01687.1"
                     /translation="MNRVLLFITIFILAFLAGNLAAQDSDCDPPEILCPTTPFEVSMC
                     GAGEVCIDLPVIESNSPVTVIAENAVVDKRASTVCYPVTSSGTYVFVIIAQNECGADT
                     CEVTAIVTINEPPVIDCPSGAVPVSICEPGPICLDLPIADADEVSASGGEWTEGAFCF
                     NADTSGTYTYLIEAANGCGTTGCEFAVNVEIVTPPVIDCPLVELSETICGPGEVCVEL
                     AITNYDEVQVDGDAVWSDGSLCFDAQTSGFYTFTVTSSNECFAAECALGINVMILEAP
                     FITCPVEPVEVLVCEGAQAGVYLPINNPGEVKVMPESAGNYWQSDTLYFDAVNPGQNF
                     FTVVAENQCGSDTCELTATVEFGTVPMIACPEDTLELLLCEADTVCIELPVENPGTVT
                     IQGYEAVWENGLLCFEPAFPMDQNITTYYDFTIIAADFCGADTCNLVAAVETVLAPQI
                     DCPEDTLEISACAGTTVEIPAAVFHAETIDILGGEYEDGIVSFTADTSQLRHIRITAI
                     NACSTNTCEFYVDLTVLHAPAPDFSIDSTSQDVTPVIVYFRNDTEISGDITFVWNFGD
                     GQTSTEFAPAHAYDSNGCYDVSLEATNQCGGAILIKEGFVCITDAQVVIPTPEWISIY
                     CGQPALDDQALQPGDIISAYDPDGVLCGMGTVRQDGSFGFLPIYRDDATSPDLDEGAE
                     PGDPISLEINFDPVFTTPRVIWTENGDRIEVCDFSTEKCSPINITMGWSLVSWNNTYT
                     AEIEEFVDLIGGPDCVRVILGFDQSALTYNPDLPEFSTLDHVDYYHGYWIYGECLPLV
                     APQVCGGAIDPQEYIPVYDGWNLVAVWLDAVLPVEDAWESIFEILMVALGYHEQGVTW
                     MPDMGEFNTLTEIRPIYGYWAKVSENGILAYPGFGIPPVHNLAQKSQSKGVRPTRQWI
                     NIYGDELKLDDRRLPSGTEIEIRAENGILCGSAVYNGELLKFTPVYGYDGSGIATADY
                     PQENAILSIYIDGQKVYPEIAWTELGDCIEVGNLFSKPGGSSPTVPDEYSLSQNYPNP
                     FNPNTVISFELPTACNVELSVFNLLGQRVASLVNGYHDAGIYESEWDGTDDDGNRVSS
                     GIYLYRIKAGNFVQTKKMLLAK"
     gene            5457..7043
                     /locus_tag="ENO07_06680"
     CDS             5457..7043
                     /locus_tag="ENO07_06680"
                     /inference="COORDINATES: protein motif:HMM:PF13440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HDS01688.1"
                     /translation="MVEKPALKLTGKLLARNTVFNLVGQLLPLVVAVITIPIIMSALG
                     VERFGVLTLAWMVIGYFGLFDMGLGRATTKFVAEYLGLGDSGSAGRLIRVSLLMLAGF
                     GIMGTAFMLILTPWLVKSVLNIPSVLLEESLHAFYLLAFSIPVVSMTAGARGILEAQQ
                     RFDIVNYIKVPMYMTAFIAPLMVLPFSNKLLPIVAVLIACRFAGLFAYLYFCARSFPG
                     MIRPSLPELGLIRQLLSYGGWLTLSHLIWPIMTYMDRFIIGAILTMAAVAYYVTPYEL
                     VSRVLVISGSILAVVFPAMSMYAREQHEKFLRLFNRAIKYLLMILVPAVFLIIAFARP
                     IFGLWLGHDFALNSAIVLQLLALGKLINSVGQVSSSAVQAMGRPDITAKLHLIELPVY
                     LVMLWLFVRQMGIVGAAIAWTARVTLDLMLNLIIFRRLSSMLSGLNPRIRLNLVSWSA
                     FMIGAAFLLSAVTAPLLNMPISIIMLGGSAMLYWYKFFENDERDFVRNIVRKLFGKGA
                     LIEANPQASVNKVERDKVKV"
     gene            7110..7370
                     /locus_tag="ENO07_06685"
     CDS             7110..7370
                     /locus_tag="ENO07_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01689.1"
                     /translation="MRKLWDIHRLPPEQLEKHQLEKLNDLLNHVYHNVEYYNKIMSGS
                     GLLKKDEIKRLMESGYRVEYEFINDIETTGTGKVQPVISEIA"
BASE COUNT         1884 a         1856 c         1773 g         1873 t
ORIGIN      
        1 ccttgacatt ctgcaccttt ccatcacgta tcaccaccgc atcctcgata tattcattta
       61 tgaggccgtc aactgagaaa acaattttat aattcagggg cggctcaggg ttttggggaa
      121 gtcccccgac tcgcaggtga acgttttcga gctcatcaaa tctggatgat cctgatgaag
      181 tcaggatctg caccagcccc ggagcaaggc cggtatccgg gataatcgtt acgcctttgg
      241 attttgcttg ttcatggagt ttcaactgtt cgcccacaac tctgttattt ccgcccagat
      301 caatgaagtt agcgccaatc tcgattgcca gcctggataa ttgcagattg aattggtagt
      361 gtatggcgga tatgatgacg ctgcagccgg aaatagtatt cttcgttccg ctcggattgc
      421 ggacatccag ccaagtcggc actaccttat ccgatttggc aaaagccgcg acctcttccg
      481 cgttcgagag atcgatatcg caaacaagaa tcttatcgat tttatcgttc tgcgccagat
      541 cataggcaat agccttgccc atcatcccgc ttccaagtaa tgcaatctgc atcaatcctc
      601 catcttttgc tgaaatcaag atataaaaaa agcgcgggat gtcaaaggtt tcgagaaata
      661 ccggggataa tcaacagccg tgcagctcga tcaatttatt tatcgtgggg gtatcggcat
      721 tacctgtggg acttaaatcg ttatcttcct ggaatctgcg aagagccctg taaagttcca
      781 tatttttctt ttccgggatg tcatggttga aataaccaag gttttttaga cggtaggcgt
      841 agcccgagac agttcgggga gggtccatat aacccatatt ccaggtcatc accttctctt
      901 ccagctccat gctcagagac tccactccgg gcggaagctc ctggtcgacc tgcccttctc
      961 cgccggtgct ggccgaaacc tcctcatcct gttccggatg tctgagggta taatcgagat
     1021 tcgtgaggga ttctccctca gtatttttca gacgtgtggt gatccgctcc ctgacagcgc
     1081 gagtcacgct aatactgaca gcacccccgt tcttgcccct ggtgggaaac tccggaacgg
     1141 gaagcaccgg gacccagact tcatctcccc gcgccagcaa attcctttcc ggacgctttt
     1201 cgcgaagata ggaattttta tcatgatcat aaatcggggc agggtcagca tgattgaaat
     1261 ctctggcgat ccttcccatc caatcaccat cacggcagac atgcagtttg tgtctttctt
     1321 cggaatccgg catacgacaa aaataaatct gctcaataca tgtgtcaagc aaagagagaa
     1381 ttcgggcgca gtattggcaa aaaaatacgg gacaggccct ggcccgtccc gctaattgga
     1441 gcaggcaaca gatttatttg gccagaagca tcttctttgt ctggacaaaa ttcccggctt
     1501 taatgcgata tagatatata ccggatgata cccggttgcc gtcgtcatct gtaccatccc
     1561 attcagattc atatataccg gcatcgtggt agccgttcac cagacttgcc accctctgcc
     1621 cgagaagatt aaacacagaa agctcgacat tacaggcggt cggcaattca aagctgatga
     1681 cagtgttcgg gttgaatgga ttcggatagt tttgcgaaag cgaatactca tccggtacag
     1741 ttgggctgga accaccgggt ttgctgaaga gattgccgac ctcaatacaa tctcccagct
     1801 cggtccaggc tatctccgga tagacttttt gaccatcaat atatatgctc aatattgcgt
     1861 tttcctgcgg ataatcggct gtggcgatac cggagccgtc atacccgtaa accggcgtaa
     1921 acttaagcag ctcgccattg tagacagcgc ttccgcaaag aataccgttt tcagcgcgga
     1981 tttcaatttc agtgcctgag ggaagcctcc ggtcatcgag tttcagctca tccccataaa
     2041 tattgatcca ttgcctagtt ggccttactc ccttgctttg agatttttgc gccaggttat
     2101 gtacgggagg tataccgaag cccgggtatg ccagaattcc attttcagac accttggccc
     2161 aatatccgta gataggacgg atttccgtca gcgtgttgaa ttctcccata tcaggcatcc
     2221 aggtgacacc ttgctcatga tatcccagag ccaccatgag aatctcgaat attgattccc
     2281 aggcatcctc gacaggcaga acagcatcca gccagacagc gacaagattc catccatcat
     2341 aaaccggtat atactcctgg ggatcgattg cgccgccgca aacctgcggg gcaaccaacg
     2401 gcagacattc accatagatc cagtagccat gataatagtc aacatgatcc agtgtagaga
     2461 attcaggtaa atctggattg taagtcaggg cgctctggtc aaatccaaga atcaccctga
     2521 cacaatccgg cccgcctatg agatcaacaa attcctcaat ctcagcggta taagtattat
     2581 tccacgacac caggctccag cccatggtga tattaatcgg ggagcacttc tcggttgaga
     2641 aatcgcagac ctcaatgcgg tcgccattct cggtccagat aacccgggga gtcgtaaaga
     2701 caggatcgaa attaatctca agtgagatcg gatcgcccgg ttcagctccc tcatcaagat
     2761 cgggcgatgt agcgtcatca cgataaatcg gcaggaagcc aaagctgcca tcctgcctga
     2821 ccgtgcccat accgcacaaa acgccgtcag gatcgtaagc gctgataata tcgcccggct
     2881 gcagggcctg atcatcaagg gctggctgtc cgcaatagat tgaaatccat tccggcgtgg
     2941 gtattacaac ctgggcatcc gtgatacata caaaaccttc tttaatcaat attgctccgc
     3001 cgcattgatt tgtcgcctcc aggctgacat cataacagcc attggaatcg taagcatgag
     3061 ccggcgcgaa ttcagtactg gtctggccat ccccgaaatt ccacacaaaa gtaatatcgc
     3121 ctgaaatttc agtatcattg cggaaataca caataacagg tgtaacgtcc tgtgaggtag
     3181 aatcgatgct gaaatcagga gcgggagcat gcagaaccgt gagatccaca taaaattcgc
     3241 aagtatttgt agaacaggca tttatagctg taattcggat gtgcctgagc tgggaggtgt
     3301 cggctgtgaa gctgacaatg ccatcctcat actctccacc caatatgtca attgtctcag
     3361 cgtgaaaaac tgccgcggga atttcgaccg ttgttccggc gcaggcgctg atttccaggg
     3421 tatcctcagg acaatcaatc tggggggcaa gaaccgtctc aactgcggca accagattgc
     3481 aggtgtcggc gccgcaaaag tcggccgcta taatcgtgaa atcataatat gtggtaatat
     3541 tctggtccat gggaaaggcc ggttcaaagc atagcagacc attctcccaa accgcctcat
     3601 agccctggat tgtcacagtt cccggatttt ctacaggcag ctcgatacag actgtatccg
     3661 cttcgcagag aagtaactcg agcgtatcct cggggcaggc tatcatcgga acggtaccga
     3721 actcgaccgt cgcggtcaat tcacaagtat ccgaaccaca ttgattttca gcaactaccg
     3781 taaagaaatt ttgacccggg ttaacagcgt caaaataaag ggtgtcgctc tgccagtagt
     3841 ttccagcgct ttctggcata accttgacct cgcctggatt gttaatcggc agatatactc
     3901 ccgcctgcgc tccttcacaa acaagaactt caactggttc aaccgggcag gtgatgaagg
     3961 gcgcctcaag gatcatgaca tttatcccca aagcgcattc agcggcaaaa cattcattgg
     4021 aagaggtaac tgtgaaggtg tagaatccgc tggtctgtgc gtcaaaacag agcgaaccgt
     4081 cgctccagac agcatcgcca tcaacctgaa cctcatcata attcgtaatt gccagttcga
     4141 cacagacctc acccggcccg cagatagtct ctgaaagctc gaccagggga cagtcgatca
     4201 ccgggggggt aacgatctcg acattgacag caaattcaca tccggttgtt ccgcagccat
     4261 ttgcggcctc gatcagatat gtgtacgtcc cgctcgtatc ggcattgaag caaaacgccc
     4321 cctctgtcca ttcaccgccg gaggcgctca cttcatcggc atccgcaatg ggtaaatcaa
     4381 gacatatcgg gcccggctca caaatagaga ccggaacagc tcccgagggg cagtcgatta
     4441 ctgggggctc attaatagtc acaattgccg taacctcgca tgtatccgcg ccgcattcat
     4501 tttgggcgat gatcacgaag acataagttc cggaagaagt aactggataa cagacagtac
     4561 tggcccgctt gtcaacaact gcattttccg cgatgacagt aacaggtgaa ttactttcaa
     4621 tcaccggaag gtcaatgcag acctcgcctg ctccgcacat gcttacctca aacggtgtgg
     4681 tgggacagag aatctccggg ggatcgcagt ctgaatcctg ggcggccaga ttgccggcaa
     4741 ggaatgccag aatgaagatg gtaataaaca gtaatactcg attcataaat tcctccctgc
     4801 ccagagggca ttactataca taaaatccat ttatatttgc gtctcggata ttactttata
     4861 aagtgccagt cagataagta tttcgtattc tgaattttcc cccgcatgct cctgagagcc
     4921 tcttttaagt gtcctgcgca tatcaaccgc ccgcgcttgc tgccgtccct ggtgttatta
     4981 gtaggtgact ctaaccggcg caagccgctt taaggatgtg tattctaatc agacgcaata
     5041 tgcttataaa ataaggatac aaagtttgag ggcttttcgc aagtactttg agaaaagtta
     5101 tgcgggactc aaaatccatt gtgatgaagg agggggcgga gaggctgatc gactgcagat
     5161 agatacctta caacaaattc accctaatcg tctccacatt ccagcccttg ctttgaacac
     5221 aactgaactg caaattcagt attttcatcg gttcaatata tttcagagtg aaaccgtctg
     5281 tttttctctg gaaaggcggt aataatacgt ttcaatctgc cgaagcatag aataagagaa
     5341 tgggaaatcg cggcaactga aactcaggga agagattagt tgaatccagg ccgacccctg
     5401 tatgggaaac cggcaaatcc gctatcatat tgcagagcga aaattcggag aatattttgg
     5461 tggagaaacc cgccttaaaa ctgacaggta agctcctggc cagaaatacg gtcttcaatc
     5521 ttgtcggcca gcttctgccg ttggtcgtgg cagtaatcac gattccaatt attatgagtg
     5581 ctctgggggt ggagcggttt ggcgtcctga ccctggcatg gatggttatc ggatatttcg
     5641 gtctcttcga catgggtctg ggacgtgcca ctacgaaatt tgtagccgag tatctgggtc
     5701 tgggcgactc aggttcggcc ggacgcctga tccgggtttc gcttttgatg ctggcgggtt
     5761 ttggaatcat gggaacagct tttatgctta tcctgacgcc ctggctggtc aagagtgtat
     5821 tgaatatccc atcggtgctc ctggaagaat cactgcatgc gttctacctc ctggcgtttt
     5881 caattccggt tgtctccatg accgccggag ccagaggcat tcttgaggcg caacaacgct
     5941 ttgatattgt caattatatc aaggtgccaa tgtacatgac cgcttttatc gccccgctga
     6001 tggttttgcc gttttccaat aaacttcttc cgatcgttgc cgtactgatt gcctgccggt
     6061 ttgccggact gtttgcctat ctctactttt gtgcgcgttc ttttccgggc atgatccgtc
     6121 cttcattacc cgagctgggc ctgatcaggc agcttttgag ttacggcgga tggcttacgc
     6181 tgtcccatct aatctggccg ataatgacct atatggaccg gtttattatc ggagccatat
     6241 tgaccatggc tgcggtagca tattatgtca ccccttatga actggtctcc agggttctgg
     6301 ttatcagcgg cagcatattg gccgttgtct ttccggcgat gagcatgtac gcccgggaac
     6361 agcatgaaaa atttctacgg ctgttcaacc gtgccataaa atatcttctg atgattctgg
     6421 tgccggccgt cttcttgata atagcgtttg ccagaccgat tttcggactg tggctgggac
     6481 acgattttgc actcaacagc gcgattgtgc tccagctcct cgcactcggg aaattgatca
     6541 attcagtcgg ccaggtctcc tcgagcgccg tgcaggctat gggacgtcct gacattaccg
     6601 ccaaactgca cctgattgag ctgccggtct atttggtgat gctctggttg tttgtcaggc
     6661 agatgggcat tgttggcgct gccattgcct ggaccgccag agtaacgctg gacctgatgc
     6721 tgaacctgat aatcttcaga aggctatcgt caatgctctc agggcttaat ccccggatca
     6781 ggttaaatct ggtatcatgg tcggccttta tgataggcgc cgcatttctt ctgtcagcag
     6841 ttacggctcc tttattaaat atgccaattt caatcattat gctgggtgga tcggcgatgc
     6901 tgtactggta caagttcttc gaaaatgatg aaagagattt tgttagaaat atagtccgca
     6961 aactgtttgg caaaggcgcc ctgattgaag ccaacccgca ggcatcagta aataaagtcg
     7021 aacgtgataa agtaaaggta tagatgctgc gagcacagaa aacattgaga aaaatctgtt
     7081 tccgaaatag aaacggggta atcaggcgca tgaggaagtt atgggatatt cataggctgc
     7141 cgcctgaaca gcttgagaag catcaacttg aaaaacttaa tgatcttctc aatcacgtct
     7201 atcataacgt tgaatactat aataagatca tgagcggcag cgggctgctt aaaaaagatg
     7261 aaataaagcg gctgatggag tctggatatc gggttgaata cgaatttatc aacgatatag
     7321 agacgacagg caccggcaag gtccagcctg taatttcgga gatcgcttag tatgaggaaa
     7381 agacaa
//
LOCUS       DSEB01000373            2277 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1007583, whole genome shotgun sequence.
ACCESSION   DSEB01000373 DSEB01000000
VERSION     DSEB01000373.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1007583"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            33..>2277
                     /locus_tag="ENO07_06690"
     CDS             33..>2277
                     /locus_tag="ENO07_06690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HDS01690.1"
                     /translation="MLPYTLDRSYVLDQSASLRAVRNPLQIWTGDSSFRDGRAMVRAS
                     NPRPTSDEFPNVSFVTMDMGTVYDQDPVPAPITDMALYYDSQAEMQAVVLGANRNDSA
                     FAVKIIPGTDNPQFLYLTHGQDHTGNGVWNTGVSIQLCEDYDYDGTEEVFVYVSPGRD
                     LYPRVLYCLDMEKLQIEWALQLASPIQKQNFFSCRDPENPAVIFTTYNVKNGVSDKNF
                     SDLYCYLAKVDSSGTVLFKKILASEHGGVFIRPAEEDTIFYVAHSLPFLDDEEAAKAV
                     EKTYKLSRIDRSGNILESVYAKEKIADMWLADYGDSDVPHLYTISHTGVISVYDPRLN
                     IWAESSPTDLRSLVGITRIADNPGPAFVMKTTEGVEIYSCDFKKLASLQFSAGNFQPL
                     EHDESGNLTAFILTNGREGGIFRLMKRGVADYSRIIFWEYQNYITVALFILLVALFVT
                     NLHRIKTIRKLRISERNLEGLFTSNPQAAIYVDTESNVLDVNLHFEETFGYKKEELLG
                     HPVIDMLVPEEERAEVYDRFKKTDSATFYEAKRARKDGTPIWVRVSGSPVEAGNKMIG
                     YIIVYNDITEIKRAQESLKENAARYRTLFEDSPIMLAEEDYSEAMKYVNELKNSGIID
                     IEGYLLEQPDILRKCQSMIRLVDINKAARKIYGAKGMDELKSHFNRMFADYFLPILAR
                     TLDQFSRGEKMIQTELTTEKVNGEEVHTIMKINVAPGYEDYFSKVFVSLLDITDRKNA
                     EDALRESE"
BASE COUNT          632 a          530 c          568 g          547 t
ORIGIN      
        1 aactatccgt tgattattcg cggaaaagta agatgcttcc ctacaccctc gaccggagct
       61 atgttctgga tcagagtgcg agccttcgag ctgttagaaa tcctcttcag atctggaccg
      121 gcgacagttc cttccgggac ggaagagcaa tggttcgggc ttcaaatcca aggccaacct
      181 cagatgaatt tccgaatgtt agctttgtaa ccatggatat gggcaccgtc tacgatcagg
      241 atccggtgcc ggcgcccatc acagatatgg ccctttatta tgattcccag gcggaaatgc
      301 aagcggtcgt cctgggagcc aaccggaacg actcggcctt tgccgtgaag attataccgg
      361 gcacagacaa tccgcaattt ctttacctca cgcacgggca ggatcatacc ggcaatggcg
      421 tctggaacac aggcgtttcg attcaacttt gcgaggacta tgattatgac ggcacggaag
      481 aggtgtttgt ctatgtgagt cccggccgcg acctttatcc cagagtgctt tattgccttg
      541 acatggagaa actgcaaata gaatgggccc tgcagttggc ctctccgata cagaagcaga
      601 acttcttcag ctgcagggat cctgaaaatc ccgcggtcat cttcacaacc tataatgtaa
      661 aaaacggcgt cagcgataaa aacttctcgg atctgtattg ttatctggcg aaggttgatt
      721 caagcggcac agtactgttc aagaaaatcc tggccagcga acacggcggg gtctttattc
      781 ggccagccga agaggacacg atcttctatg ttgcccactc gctgccattt ctcgatgatg
      841 aagaggccgc taaggcggtt gaaaaaacat acaagctctc acgcattgac cgcagcggca
      901 acatcctgga atcggtatac gcaaaagaaa aaatagccga tatgtggctg gcggactacg
      961 gtgactccga cgttccccat ctctacacga tctctcatac cggcgttatt tctgtttatg
     1021 atccccgcct gaatatctgg gcggaatcga gtccgaccga cctgcgctcg cttgtgggca
     1081 ttaccagaat agcagataat cccggtcctg catttgtaat gaaaacgacc gagggggttg
     1141 agatatattc ctgtgacttc aaaaaattgg cctcgctgca attttctgcc ggaaatttcc
     1201 agccgctcga acatgacgag tccggcaatc tcaccgcttt cattcttaca aatgggaggg
     1261 aggggggaat cttccggttg atgaaaaggg gcgtcgctga ttactccaga ataattttct
     1321 gggaatatca gaattatata acggtcgccc tgtttattct gctggtggcg ctttttgtca
     1381 cgaatctgca tagaataaag actatcagaa aactgcgcat cagcgaaaga aacctggaag
     1441 gtctgtttac ttctaatccg caggcggcaa tctatgttga taccgagagc aatgtgctcg
     1501 atgtcaacct tcattttgaa gaaaccttcg gttataaaaa agaagaactt ctcggtcatc
     1561 cggtaataga tatgctcgtt ccggaggaag aaagggccga agtatatgat cgtttcaaga
     1621 aaactgactc tgccactttc tacgaggcga aacgggccag aaaagacgga acgccaatct
     1681 gggtgagagt atccggctcg ccggttgagg caggcaataa aatgattggc tatattatag
     1741 tctacaatga cataacggaa ataaagagag ctcaggagag cttgaaagaa aatgcggctc
     1801 gctatcgcac cctcttcgaa gactcgccca taatgctggc ggaagaagat tatagcgaag
     1861 ccatgaaata tgtgaatgaa ctgaaaaact ccgggatcat tgacattgaa ggatatctac
     1921 ttgagcagcc tgacatacta agaaagtgcc aatccatgat caggctcgtt gacatcaaca
     1981 aggccgcccg caagatttac ggggcaaagg gtatggatga actgaaaagt cacttcaaca
     2041 ggatgtttgc cgattatttc ctgccgattc ttgcgcgtac attggatcaa ttttcccggg
     2101 gagagaagat gatccagacc gaattgacga ctgagaaagt taacggcgaa gaagttcata
     2161 ccattatgaa gataaatgtc gcgcccgggt atgaagatta tttctctaaa gtctttgtct
     2221 ccttattgga tataactgac cgaaagaacg ccgaagatgc cctgcgggaa agtgagg
//
LOCUS       DSEB01000374            1748 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1013636, whole genome shotgun sequence.
ACCESSION   DSEB01000374 DSEB01000000
VERSION     DSEB01000374.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1013636"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..731
                     /gene="truA"
                     /locus_tag="ENO07_06695"
     CDS             <1..731
                     /gene="truA"
                     /locus_tag="ENO07_06695"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDS01691.1"
                     /translation="YKITIEYDGGGFNGWQVQPGLRTVQGEIEKAVEEISGRKSNVIC
                     AGRTDAGVHAFGQVANFKSRIDWGDERVLKALNRVLPDDIVILECEEVGSDFNARYSA
                     LARHYEYRIYNARSSIRRNFYWQYPARINLACLKRLSAMIMGEHDFKSFCVRKSQKES
                     NLCTVTKSHWTKRDKEYTFQIIANRFLHGMVRSLVGTMIKISAGFSEEEVFEELLAKP
                     KRSEKVFTAPPNGLYLMEVAYEIE"
     gene            779..1429
                     /gene="fsa"
                     /locus_tag="ENO07_06700"
     CDS             779..1429
                     /gene="fsa"
                     /locus_tag="ENO07_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDS01692.1"
                     /translation="MKFFIDTANIEEIKAANDMGVLDGVTTNPSLISKVEGKFEDIIL
                     EICNIVDGPVSAEVTATDFEGMMTEARHLAKIHKNVVVKIPCILEGLKATKALNDEGI
                     RVNMTLVFSPTQALLAAKSGATFCSPFVGRLDDISEYGMDLVEKIVTIYNNYSFNTEV
                     LVASVRNPLHVVEAAMMGADIATIPFKVISQLVKHPLTDIGLKKFLDDWEKVKARG"
     gene            1426..>1748
                     /locus_tag="ENO07_06705"
     CDS             1426..>1748
                     /locus_tag="ENO07_06705"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017552970.1"
                     /note="Catalyzes two discrete reactions in the de novo
                     synthesis of purines: the cleavage of adenylosuccinate and
                     succinylaminoimidazole carboxamide ribotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDS01693.1"
                     /translation="MISRYTLPEMGNLWTDESKFGAWLKVEIAACEAWAELGEIPNAA
                     VNRIKKKASFNVKRIRKIEEKTRHDVVAFVTNVAASVGEDGKYVHYGLTSSDVLDTAN
                     ALLLKG"
BASE COUNT          463 a          379 c          483 g          423 t
ORIGIN      
        1 attataaaat cactatagaa tatgacggcg gcggtttcaa tggctggcag gtgcagcccg
       61 gcttgcggac ggttcagggc gagatcgaaa aagctgtgga agagatttca ggacgtaaat
      121 cgaatgttat ctgcgccggc cgcacagatg caggcgtgca cgccttcggg caggtggcaa
      181 attttaagtc aagaatcgac tggggtgatg agagggtcct caaggcgctt aatagagtgc
      241 ttccggatga tatcgtcatc ctggaatgtg aagaggtcgg ttcggatttt aatgcgcgct
      301 attccgcatt ggcaaggcat tacgagtata gaatatataa tgcccgaagc agtatccgcc
      361 gtaacttcta ctggcagtat ccggcccgga ttaatcttgc ctgtttgaag cggctttcgg
      421 ctatgataat gggtgagcat gatttcaagt cattttgtgt caggaaatcg cagaaagaat
      481 ccaatctctg cacagtcaca aaatcgcact ggacaaagcg ggataaagaa tataccttcc
      541 agattattgc caaccgcttc ttgcacggta tggtgcggag cctggtgggc acgatgataa
      601 aaatctcggc cggattctcg gaagaagagg tttttgagga gcttttggca aagcctaaaa
      661 gatcggagaa ggtctttacc gctccgccca atgggcttta tttgatggaa gtcgcttacg
      721 aaattgaata atgatcttat ttgagggttc gagcccgata cagaatggag gcttataagt
      781 gaaattcttc atcgataccg ccaacataga agagataaag gcagccaacg acatgggcgt
      841 cttagacggc gttaccacaa atcccagcct gatttcaaag gtcgagggta aattcgaaga
      901 tataattctt gagatctgca atattgttga cggtcctgtt tcggccgagg tcacggccac
      961 ggattttgag ggcatgatga cagaagcccg gcaccttgcc aaaattcata aaaatgttgt
     1021 ggtcaaaatc ccctgcattc tggagggcct caaggccacc aaagccctga acgacgaggg
     1081 catcagggtc aacatgaccc tggtattttc tcccacccag gcgcttctgg cggccaagtc
     1141 cggggcgaca ttctgttcgc cattcgtcgg ccgtctggat gatatctccg aatatgggat
     1201 ggacttggta gaaaaaattg tcacgatata taataattac agtttcaata cagaagttct
     1261 ggttgcctct gtaagaaacc cattgcacgt ggtcgaggcc gccatgatgg gtgcggatat
     1321 tgcaactata ccgttcaagg tcatcagtca gctggtgaag catccgctga ccgatatcgg
     1381 cctcaagaaa ttcctggatg attgggagaa agtgaaggcg agaggatgat ttctcgctac
     1441 actctgccgg agatgggcaa tctctggaca gatgaatcga aattcggggc ctggctcaag
     1501 gtggaaattg ctgcctgtga ggcctgggca gaattggggg agattcccaa tgctgcggtc
     1561 aacaggatta agaaaaaggc atcatttaac gtgaagcgta tccgcaagat cgaggaaaag
     1621 acccgccatg atgttgtggc atttgtcacc aatgtggctg cgagcgtggg cgaggacggt
     1681 aagtatgttc actacggcct gacttcatcg gatgtcctgg ataccgccaa tgctctgctt
     1741 ctcaaggg
//
LOCUS       DSEB01000375            7336 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000576, whole genome shotgun sequence.
ACCESSION   DSEB01000375 DSEB01000000
VERSION     DSEB01000375.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7336)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7336)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7336
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000576"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            421..2487
                     /locus_tag="ENO07_06710"
     CDS             421..2487
                     /locus_tag="ENO07_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01694.1"
                     /translation="MKKSQFILIILFALILAPHIFGQVPHIMNYQGRLLTGSGEPVPD
                     DVYDITFSIYNDPVTVDHLWQETLSVEVSGGFFHVNLGDVAPFEDLFFANGPELLLGI
                     KIGSDPELSPRTSITASGFALHSELADISHLALNVEPNTLTGEHIVDGTLGLEEINQS
                     GALEGQVPKWLEGTWVPADDEGGGGLELPYSGAVPTAADAFSIFNAGSGVAVRGQSDG
                     NDGVVGWTADSSMSGVYGYSSTGIGVTGRSEGYNHGVYGETGSCNLNDAGVRGVSLCD
                     APGVSGYGPAIGIYGSSPSVAGRFDGFVGINGFQATANDTGTAAFFESEHMTLDDTAV
                     VYIKYDGDSDPNLGMPTALTAKCFDPNNRSVGGYFVASGIGLLSISADTLDNHSNHGL
                     ITRIGYGGRNVGVDVWTLFDPIRPQPADNFGAWLESHDAEYNFGLLARAGDEYLTPHK
                     PKYNYGVYAEGTEADSMGIGVYGYAYDCDSTIGMYASAFGGNVNIGLYAKAPSGPNDW
                     AGYFDGDVHVTGNLSATSKSFRMDHPQDPANKYLSHACMESSEMLNVYSGTIVLDGNG
                     EAAVALPDYFEDINKDFRYQLTAIGAPGPNLYIAEEINNNSFKIAGGNPGMKVSWEVT
                     GVRNDPYAQAHPMQVEIEKNDREKGKYLHPEAYGLDESYGIHTSARREFPERDEPDWL
                     EDIGKR"
     gene            2699..4759
                     /locus_tag="ENO07_06715"
     CDS             2699..4759
                     /locus_tag="ENO07_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01695.1"
                     /translation="MKKCQFTLSILVVLLLAPHVFGQVPHIMNYQGRLLTSSGEPVPD
                     NVYDITFSIYNDPVTVDYLWQETLSVEVSGGFFHVNLGDVAPFEDLFFANGPELLMGI
                     KIGSDPELSPRTSITASGFALHSELSDISHLALNVEPNTITGEHVVNGSLGLDELGQS
                     GASEGQVPKWLEGAWIPADDEGGGGGLALPYAGDIAAPADAFSVLNGGTGVAIRGQSA
                     GNDGIVGWTADSSKSGVYGYSGAGIGVTGRSDGERYGVYGESNSDSAAVFGRNFGDGP
                     GVKGYSPQAAIYGSSPGYAGYFDGNLSVLGRQETTKDTGTAAFFESNYLAPEDSGVVN
                     IAYDGDSDPTFSTPMALVAKCFDPNNRSVGGYFQASGLGLVSISADTGQSDGNCGLLA
                     KVGYGVQNVGVEVYTLSFPTYPQSGSNFGVWSETKDGLYNIGVRARAYDDISTSHRTR
                     YNYGIFSLAENADSTGIAVYGRAYYCDSTIGMYADTYGGAVNIGLFARAPSGANDWAG
                     YFDGDVWITGNVWAADKFFKTDHPQDPANKYLTHASMESSEMLNVYSGTIVLDGNGEA
                     AVALPDYFEDINKDFRYQLTAIGAPGPNLYIAEEINNNSFKIAGGNPGMKVSWEVTGV
                     RNDPYAQAHPMQVEIEKNDREKGKYIHPELYGLDDSYGIQTNARREAPQRNAPEWLEE
                     IGKK"
     gene            4780..6327
                     /locus_tag="ENO07_06720"
     CDS             4780..6327
                     /locus_tag="ENO07_06720"
                     /inference="COORDINATES: protein motif:HMM:PF06253.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01696.1"
                     /translation="MNLLAQTGYISLYIPYIFVNDRYSAEGDNMRPTVEFLEEGLLER
                     IVSEAIDILCKIGVEIHNKPVLEMLADHGADVRMDNYRATLTEKIINKALVSAPSSFK
                     LYDSMGNEAVDLSEDNVNFTPGSAAINILDYETGKIRKPTTSDYIRYAKLMTRMDHIA
                     SQSTAFIPADVEAKISDSYRLFLSLLYCEKPVVTGAFTIDAFEIMKDLQLAVRGSGEE
                     LAKKPLTIFSCCPTSPLKWSDVTSQNVVDCAKYMIPVEYIAMPLAGFMSPVTLVGTLV
                     QHTAETLSGLAISQLTNPGTPVLYGGSPAIFDVRYETTPMGAIETQMIDCAYNEIGKY
                     LDLPTQAYIGLSDAKLLDAQAGLETSIGAILAALSGINNISGPGMLDFESCISLEKLV
                     LDNQICGMTQRLIRGIVPKEDFPMIDRMEELLHEQHLLISDHTMKYLNEEHFMPSQTI
                     DRANRSRYEEEGGLTLNQRAHQEAEEHLAGYKKPGLADDTVKDLVRLMEEEAKKYGMD
                     KLPEREE"
     gene            6324..7037
                     /locus_tag="ENO07_06725"
     CDS             6324..7037
                     /locus_tag="ENO07_06725"
                     /inference="COORDINATES: protein motif:HMM:PF02965.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01697.1"
                     /translation="MTEIIKFDMNDIIPGREEVLRNQGVIPGAVLEDKIRDLLEQAIR
                     EFQSLAEPVGVMKEVSIGDFKDIYKGEGRNDEPTPVADIFPKADCMALFAVTLGQKIS
                     DKIDQHFKANNFAEGCMLDAGASAGADQAAEIMQRMYEQEIAERFDDFDAIGVVRYSP
                     GYCGWHISAQKKLFGFLGPEKIGITLRETYLMDPLKSVSGVIIAGPKEIHQFENNYPC
                     CENCDDQTCLERMQALKNA"
     gene            7055..>7336
                     /locus_tag="ENO07_06730"
     CDS             7055..>7336
                     /locus_tag="ENO07_06730"
                     /inference="COORDINATES: protein motif:HMM:PF02607.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HDS01698.1"
                     /translation="MDILKQISESLQKGDHEKVAMLTSQAIEEKIPVKKILDEGLIAG
                     MNVVGEEFKHHRIFLPSVLLAARAMYAGMDLIKPLMIRDNIPTIGKVVIG"
BASE COUNT         1942 a         1500 c         1950 g         1944 t
ORIGIN      
        1 gtatcggggc cctcatatat atgcggcgat gccaataatg acgccaatgt gaatgtcagc
       61 gacgctgttc atataatcgg ttatgttttc gtgggcggaa tgtctcccat tccgtatgaa
      121 tccggcgact gcaactgcga cggcacctgc aatatctccg atgcggtctg gatcatcaac
      181 tatgtcttcg tgggcggcga tgatccctgc gacatcaatg gcgacggcga cccggactgc
      241 tgagccctcc tgtcttttta cgcatattta ctgagttagt cttattgaat tgacaagatt
      301 cagtaaattg ttatattttt ttcacatacc ttgtattaca acttcgggcg catatatgat
      361 gacaggttat ctgtctcatt cttttgacga aatctacaaa ctgctttcag gagggtggtc
      421 atgaaaaagt cccagtttat tctaatcatt ttgtttgctt tgatcttagc accacacatt
      481 tttggtcaag ttccgcatat catgaattat caggggaggt tgctgaccgg ttccggcgag
      541 ccagttccgg atgatgttta tgacatcaca ttctcgattt acaatgatcc tgttacagtt
      601 gatcatctct ggcaggagac attatcggtg gaggtatcag gtggtttttt ccatgtgaac
      661 ttgggagatg tggctccttt tgaggacctg ttttttgcca acggtcccga gttgcttttg
      721 gggattaaga ttgggtccga tcctgagctt tcgcctcgta cgtcgataac tgcgagcgga
      781 tttgcgcttc attccgagct tgcggatatc tcccatcttg ctttgaatgt tgaacctaat
      841 acgctaactg gggagcatat agtagatggt acacttggcc tggaagagat caatcagagc
      901 ggagccttgg aaggtcaggt tcccaagtgg ctggagggta catgggtacc cgctgacgac
      961 gaaggcggag gaggtctgga gcttccttat tcaggcgcag tcccgacagc ggcggatgca
     1021 ttttcaatat tcaatgcggg ctcaggtgtg gcagtacgcg ggcagagcga cggcaatgac
     1081 ggtgttgtcg gatggacggc cgattcctct atgagcggag tctacggata cagcagtaca
     1141 ggaattggcg taactggacg cagcgaaggg tataatcacg gtgtctatgg agagactggt
     1201 tcatgcaatc ttaatgatgc tggagttcgc ggggtgagct tatgcgacgc tcctggtgtg
     1261 tctggatatg gacctgccat cgggatctat ggatcttcac cctcagtagc tggacgtttt
     1321 gatggttttg tcggcatcaa tggctttcaa gccacggcaa acgatactgg aactgcggct
     1381 ttttttgaaa gcgagcacat gacattagat gatactgcag ttgtctatat caaatatgat
     1441 ggcgacagtg accctaacct tggaatgcct acagcactta ctgctaaatg ctttgatccc
     1501 aacaatcgtt cggtaggggg gtattttgtt gcaagcggaa ttggccttct ttcaatctca
     1561 gcggatacat tggataatca tagtaatcat ggattaatca ccaggatagg gtatggcgga
     1621 agaaatgttg gtgttgatgt ctggactttg ttcgatccta ttaggcccca gccggcagac
     1681 aactttggtg catggctgga atcgcatgat gcagagtata actttggatt gctggctcga
     1741 gccggtgatg agtacttaac cccacataag cccaaatata attatggcgt ttatgcagaa
     1801 ggcacagaag ctgacagcat gggaatagga gtttatggct atgcatatga ttgtgactca
     1861 acgatcggga tgtatgctag tgctttcggc gggaatgtaa acatagggct ttatgcaaag
     1921 gccccttcgg gtccaaatga ctgggcgggc tattttgatg gcgatgtcca cgtcacaggt
     1981 aatctctcag cgacatcgaa aagtttccgt atggaccatc cacaggaccc tgcaaataaa
     2041 tatctttctc atgcctgcat ggaatcttcg gaaatgctga atgtgtattc aggaacaatt
     2101 gttctcgatg gcaatggtga agcagctgta gctttgcctg attattttga agatattaat
     2161 aaggacttcc ggtatcaact gaccgctata ggtgctccgg gacctaatct ctatattgcc
     2221 gaagagatca ataataacag ctttaagatc gctgggggaa atccgggcat gaaggtctcc
     2281 tgggaggtga ccggtgtgag aaatgatccc tatgcgcagg cccatccgat gcaggtcgag
     2341 atagaaaaga atgatcgcga aaaaggtaag tacctgcatc cggaagccta cggtttggac
     2401 gagtcatacg gcattcacac cagcgccagg cgggagtttc cggaaagaga tgaacccgat
     2461 tggcttgagg atattggcaa gagatagaac atcaaagttg gatttgaact atctatgtaa
     2521 gaggtagatt ctgaaatata tttcaagttc ctaattctta ttgaattgac aaaactcagt
     2581 aaaaactggt atatttttcc gcacaccctg aattgcacct ttgggcgcaa gtgcgtaacg
     2641 tcagcatatt gctttctttg ctgacacatt caataaacag ttttcaggag ggttggccgt
     2701 gaaaaagtgc cagtttactc tatctattct ggtggttttg ttattagcac cacatgtttt
     2761 tggtcaggtt ccgcatatta tgaattatca ggggagattg cttacgagtt ccggtgagcc
     2821 agttccggat aatgtttatg acatcacatt ctccatttac aatgatccag ttacagttga
     2881 ttatctctgg caggagacat tatccgtgga ggtatcaggt ggttttttcc atgtgaactt
     2941 gggagatgtg gctccttttg aggacctgtt ttttgccaat ggtccagagt tgcttatggg
     3001 gattaagatt gggtccgatc ctgagctttc gcctcgtacg tcgataacag cgagcggctt
     3061 tgcgcttcat tcggagcttt ccgatatttc tcatcttgct ttgaatgttg agccgaacac
     3121 gatcactggt gagcatgtgg ttaatggttc acttggactg gatgaattag gccagagtgg
     3181 tgcttccgag ggtcaggtgc ccaagtggct ggaaggcgcc tggataccgg ccgatgatga
     3241 aggaggcggg ggcggccttg ctctcccata tgcgggtgat atagcggcgc cggcggatgc
     3301 attttcggtt ctcaatggcg gcacgggtgt ggctatccgg ggtcagagcg ctggcaatga
     3361 tggtatagtt ggttggacgg ccgattcctc caagagcggg gtttatggtt acagcggtgc
     3421 tggaattgga gtgaccggac gcagcgacgg agagaggtat ggggtatatg gtgaatcaaa
     3481 ttcagattca gctgctgttt ttggaaggaa ttttggtgat ggaccaggtg taaagggata
     3541 ttcgcctcag gcagccatat atggttcttc tcccggatat gctggctatt ttgatggaaa
     3601 cttaagtgtc cttggtagac aggagacaac aaaagatacc ggaactgcgg ctttttttga
     3661 aagcaattat ttagcgccag aagactcggg agtagtaaat atcgcatatg atggcgatag
     3721 tgatcctacc ttcagtacac ctatggcgct agttgccaag tgttttgatc cgaataatcg
     3781 ctctgtaggc ggttattttc aggcaagcgg gctcggcctt gtgtctattt cggcggacac
     3841 agggcaatcc gacgggaact gcggcttact ggctaaagtc gggtatggtg tacaaaacgt
     3901 tggtgttgaa gtttatacac tgagttttcc aacatatcca caatcgggtt ctaattttgg
     3961 agtctggtca gagaccaaag atgggcttta caacattgga gtacgtgcac gtgcttacga
     4021 tgatatttct acttcacata gaacaagata taactacggc atcttttcat tagcggagaa
     4081 tgcagatagc actggaattg cagtttatgg acgcgcgtat tactgtgact ccacgattgg
     4141 aatgtatgcg gatacatatg gaggtgcggt gaacatcggc ctgtttgccc gcgcgccctc
     4201 gggggctaat gattgggcag gttattttga tggtgatgta tggataactg gcaacgtctg
     4261 ggccgcagac aagtttttca agacggatca tccacaggac cctgcaaata aatatcttac
     4321 acatgcatct atggaatcat cggaaatgct gaatgtctat tcaggcacaa tagttctgga
     4381 tggcaatggt gaagcagctg tagctttgcc tgattatttt gaagatatta ataaggactt
     4441 ccgttatcag ttaacggcta ttggtgcacc gggacctaat ctctatatcg ctgaagagat
     4501 caataataac agttttaaga ttgcaggagg aaatccgggc atgaaggtct cctgggaggt
     4561 gaccggagtc aggaacgatc cctatgcgca ggcccatccg atgcaggtcg agattgagaa
     4621 gaatgatcgc gaaaaaggca aatacattca tccggagctt tatggcctgg atgattcata
     4681 cggtattcag actaatgcca gaagagaagc tccccaaaga aacgctcctg aatggctcga
     4741 ggagataggc aagaaatgat ctgataagtc cagccatcta ttaatttgct tgctcaaacg
     4801 ggatatatat ctttatatat cccgtatatt tttgttaatg acaggtattc ggcagaggga
     4861 gataatatga ggccaactgt agagtttctg gaggaaggac tgctcgagag gatagtatcg
     4921 gaggcgattg atatactttg caaaatcggg gtcgagatac ataataagcc agtgctggaa
     4981 atgcttgcgg atcatggcgc cgatgtcagg atggacaact accgcgccac attgacagaa
     5041 aagataatca acaaagcgct cgtatcggct ccaagctcgt tcaaacttta tgacagcatg
     5101 ggaaacgaag ccgtagacct ttcagaagat aatgtaaatt ttactcccgg ttcagccgca
     5161 atcaacattc ttgattatga gacaggcaaa atccgcaagc ccacaacttc ggattacatt
     5221 cgctatgcca aattgatgac ccgcatggat catatcgcct cgcaaagcac agcctttatc
     5281 ccggccgatg tcgaggcaaa aatctcagac agctatcgtt tgtttttgag cctgctgtac
     5341 tgcgagaagc cggtggtaac cggagcgttt acaatcgacg cgtttgagat aatgaaagat
     5401 ctccagctgg cagttcgtgg atcgggggaa gaacttgcca aaaaaccctt aacgatattt
     5461 tcctgctgcc caacctcgcc tcttaagtgg agtgatgtta cctcccagaa tgttgtggac
     5521 tgcgcaaaat atatgatccc ggtggaatat atcgccatgc ctctggccgg ttttatgtca
     5581 ccggtaacat tggtggggac tctggtacag cataccgcgg aaacattgag cggcctcgcc
     5641 ataagccagc ttacaaatcc ggggactcca gttttatatg gcggttctcc ggccatattt
     5701 gatgtgcgct atgagaccac acccatgggt gcaatagaga cgcagatgat agactgcgct
     5761 tacaatgaaa ttggcaaata tctcgatctt cccacgcagg cttatatcgg tctttccgat
     5821 gccaaattgc tggatgcaca ggccggactg gagacatcaa tcggtgcaat ccttgcggca
     5881 ctatcgggta tcaataatat ctccgggccg ggcatgctcg atttcgaaag ctgcatcagc
     5941 cttgaaaaac ttgttctgga taatcaaatc tgcggcatga cccagcgttt aataagggga
     6001 atagtaccga aagaagattt tccaatgatc gacaggatgg aagagctttt gcatgagcag
     6061 catcttttga tctcggatca tacaatgaaa tatctgaatg aggaacattt catgccgtca
     6121 cagacaatcg accgtgccaa ccgttcccgc tatgaggagg agggaggcct gaccttgaat
     6181 caacgtgcac atcaagaggc cgaggaacat ctggctgggt acaaaaagcc ggggcttgcg
     6241 gatgatactg taaaagattt ggttcgattg atggaagaag aagcgaagaa atatggtatg
     6301 gacaagctac ccgaaagaga agaatgacag agatcatcaa gttcgacatg aatgatataa
     6361 ttcccgggag agaagaagtc ctgcgaaatc aaggtgttat ccccggcgct gtactggaag
     6421 acaagatccg ggatcttctc gagcaggcta tccgggaatt tcaatctctg gccgaaccgg
     6481 ttggggttat gaaagaagtt tcaattggtg atttcaagga tatttacaaa ggtgagggcc
     6541 gaaacgacga acccacgccg gtggcagata tttttccgaa agcggactgc atggcgcttt
     6601 ttgcagttac actggggcag aagatcagcg ataaaatcga tcagcacttc aaggcgaaca
     6661 actttgccga gggctgtatg cttgatgctg gggcctccgc gggcgcagac caggctgcag
     6721 agatcatgca gagaatgtat gagcaggaga tagcagaaag atttgatgat tttgatgcga
     6781 tcggtgttgt acgttacagt cctggctatt gcggatggca tatcagcgcc caaaagaagc
     6841 tgtttggatt tctgggacct gaaaagatag ggataacatt aagggagact tatctgatgg
     6901 atcctctaaa atcggtatcg ggagtgatta tagcgggacc aaaagaaata catcaatttg
     6961 aaaataatta tccctgctgt gagaattgtg acgatcagac ctgcctggaa cgtatgcagg
     7021 cgcttaagaa tgcataagta aaaggagaca tagattggat atattgaaac agatttcaga
     7081 aagcctccaa aaaggagatc atgaaaaagt agccatgttg acatcacagg caatagaaga
     7141 aaaaatacca gtaaagaaaa ttcttgatga gggcctgatt gccggaatga atgtagtggg
     7201 ggaggaattc aaacatcacc ggatctttct gcccagcgta ttgctcgcag cgagggctat
     7261 gtatgccggt atggatctga tcaaaccttt gatgatcagg gataatattc caacaatcgg
     7321 caaggtagta atagga
//
LOCUS       DSEB01000376            5950 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000901, whole genome shotgun sequence.
ACCESSION   DSEB01000376 DSEB01000000
VERSION     DSEB01000376.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5950)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5950)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5950
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000901"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            440..922
                     /locus_tag="ENO07_06735"
     CDS             440..922
                     /locus_tag="ENO07_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDS01699.1"
                     /translation="MEGIQIRPLNKKDRGWLKQFMVDAWGAEKVVSRGIVHEAWLLPG
                     FTASIGGDLAGIVTYNIQDDQCEIVTLDSMIENSGAARANIQAVIDAARSEGCKRLWL
                     ITTNDNIHAIRYYQKRGFTIAAIHCNAIEHSRKLKPQIPHIGMEGIPIRDEIEFEMML
                     "
     gene            926..1900
                     /locus_tag="ENO07_06740"
     CDS             926..1900
                     /locus_tag="ENO07_06740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDS01700.1"
                     /translation="MDNFSITVEQDGEGERIDHYLVVSTKGLSRTKIQNLIKEGFITV
                     NNNTVKANFRLSAGDEIEGTIPPVREFELKPETIPLDIRFEDEYLLVINKPAGLVVHP
                     GTGNWAGTLMNGLLHYLGEKGELSENKRPSLVHRLDKETSGLLIAAKDEQSLNYMQLE
                     LKKRRIKRRYQALVWGHLREEQAVINFPIGRLPTDRRKMTVDEESGRDAVTRYVLLER
                     YKFMDHVDISLQTGRTHQIRVHFSHLGHPVVGDSDYGGNDKILQGLFDQYRMDARKVL
                     AIMRRQALHAWRLEFRHPQTGLIHAVKSELPDDIMKVLSYIKEIDPKL"
     gene            1918..2421
                     /locus_tag="ENO07_06745"
     CDS             1918..2421
                     /locus_tag="ENO07_06745"
                     /inference="COORDINATES: protein motif:HMM:PF10007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2250 domain-containing protein"
                     /protein_id="HDS01701.1"
                     /translation="MAKKGIYLAEGYKLAKCCNPEPGSQICGYYSYNNEMVVHKKGCK
                     NLAKVEKERLVDLKWKEILAAAPEMPEGDFANLTELDFEVLKHHRDYGNDYSLKVARM
                     LHKPKQDIFDSHAKLREMNLLERVKPLIIQYRKGVVDNKWIKHRNHTYYDLTEKGKLY
                     LSHYLHE"
     gene            2459..4156
                     /locus_tag="ENO07_06750"
     CDS             2459..4156
                     /locus_tag="ENO07_06750"
                     /inference="COORDINATES: protein motif:HMM:PF01523.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U62"
                     /protein_id="HDS01702.1"
                     /translation="MRERTKVLRLAAACVLTLLFASPTFAATESELLATLQEELLYNF
                     DKLKNADTVPLYYLSYQVIESQEYNVSAELGALESEDDSHVRDLDVIARVGSYELDNS
                     REVPGAGGGLSTGAVDLPLGNDPVALKMKIWEETDRRYKEVQKQYTNVITGTTVKVEA
                     EDQSDDFSREEPSIYIGDVAGLDYDRQIWEDKLVRYSSIFSNYPEIENSSVSLSARTR
                     NSYLVTTEGTKIQDGQSYIRLNIMCSSTADDGMVLRRFEGFDAADFSNMPDDETVITA
                     INRLIDELLALKSAPLAEPYTGPAILTGRASGVFFHEIFGHRMEGHRQKSESEGKTFT
                     DKVGESVLPEFISVFSDPTMRTFNGIDLRGHYKYDDQGIEARRVTLVDKGILETFMLS
                     RTPIENFPHSNGHGRKYVGFAPIARQANLIIESEKSVGFDELKEMLIEECKLQGKPYG
                     LIFQDISGGFTFTGRFLPQAFKVIPLMVYKVYVEDGHEELIRGVDIVGTPLTSFSKIM
                     IAGNDPDVFNGTCGAESGWVPVSAISPSLLLSEIEIERSQKGQERPPLLPSPLTKQQ"
     gene            4179..5918
                     /locus_tag="ENO07_06755"
     CDS             4179..5918
                     /locus_tag="ENO07_06755"
                     /inference="COORDINATES: protein motif:HMM:PF01523.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01703.1"
                     /translation="MKSIIHKFMLTLLSVALVLISSQALIAQDDPLIRALSDEMERAT
                     GSLRLEDEEPPYFLGYLANLEDNCRIRAEYGSVIECDCQKANQMFLDLRVGDYEFDNT
                     NVMTSFFGVGMAMANLPVEIVYAPLRKDAWLQIDEAYKRAVETIARKRAMLQSIIPDD
                     TVADLSKAEVVIDMKPDLYSRVDTTEWKEILAEMSAVFKNYPEFTRSSVQLNTKTENR
                     YVLNSEGTRVRRGRHVHYIFIEASTLDKNGIPVYQYDRIIVDDIDDFPAGNELVEWAD
                     DFARASAAMVDAETTDTYIGPVLFTPRASAQLLSQLLVDNISNPRKPLTMDNRFDQYL
                     SDARLVRKLNFRIMPEFISIVDDPTIRQYNGLKLKGYYEFDDQGVAAQRITLVEEGKL
                     RNYYMSRTPTEKIENSNGHGRLIQSRIGAVEVVGKPGNVIMQAAETYQPEDLKEQMLE
                     LCREMGLEYGLIIDRMNFSGRSNPDEGMRFRMTGESGIPRVIVAYLVSAEDGSITPVR
                     SLEFDNINERVLKDILAVGNDFEEATIMFDAAFNNLASIVVPSILFEEMELQKASLQA
                     KKPPIVLNPLERE"
BASE COUNT         1645 a         1396 c         1550 g         1359 t
ORIGIN      
        1 aattggttcc actatcaaaa cttggataat cgggaaatgt tcagttgtat aaactgtttt
       61 cccgctcgcg cacacggata acgggcatct aagacacagc gacagttgcc tttacgccgc
      121 accaggagag gcaatgaaaa ttccgaaatc tatttatttg ccgccggagg gaagtcctcc
      181 gggcacatgt tcttcttcgg ttttatcgct gtcgacaact ttgtcactac cgcagccgct
      241 cgagattgcg agtaaaagaa gcgttagcag gagcagaact gtttttttca atgatcttac
      301 ctttgagttt gcaaacttcc cgttgtaaat gaataagtaa aacgtaccaa cctggacatt
      361 tgtttaaaat aaattttgct tgacgccaaa aaatcccggt tctatactct tagcgggttg
      421 cctggaaagg aattacaata tggaaggcat acagatacgg ccattgaata aaaaagacag
      481 gggatggctg aagcaattca tggttgatgc ctggggggcc gaaaaagttg tctcgcgcgg
      541 gattgtccat gaagcctggc tcctgccagg attcacagcc tcgatcggcg gagatctggc
      601 aggaatagtg acttataata ttcaggatga tcaatgcgag atcgtgaccc tcgacagcat
      661 gattgaaaac tcgggtgcgg cccgggccaa tattcaggcg gtgatcgatg ctgccagaag
      721 cgagggctgc aaaaggctgt ggctgattac gaccaacgac aatatacatg caatcagata
      781 ttaccagaaa aggggattca caattgcggc catccactgc aatgcaatcg aacactcccg
      841 caaactgaaa ccacagattc cccacatcgg catggaaggt attcccatta gagacgaaat
      901 cgaatttgag atgatgttat aatttatgga caatttttct atcacagttg agcaggatgg
      961 tgagggtgaa agaattgatc actacctggt agttagcaca aaaggccttt cccgtaccaa
     1021 aatccagaat ctcataaaag aaggatttat tactgtaaat aacaatacgg tcaaagcgaa
     1081 tttcaggctg agcgcgggag acgaaatcga gggaacgatt ccgcccgtta gagaattcga
     1141 gctgaagccc gagaccatcc cgctcgatat caggttcgag gacgaatatc ttctggtcat
     1201 aaacaaacct gcaggccttg tggtgcatcc cggcacaggc aattgggcgg ggaccctgat
     1261 gaatggcctg ctgcactatc tgggcgaaaa gggtgaattg agcgaaaaca aacgtccctc
     1321 gctggtacac aggcttgaca aggagacctc gggcctcctg attgccgcca aggacgagca
     1381 gagcctgaac tatatgcaac tggaattgaa aaaaaggcgt attaaacgcc gataccaggc
     1441 attggtctgg ggacatttgc gggaagaaca ggcagtgata aatttcccta tcgggcgtct
     1501 ccccaccgac cgccgcaaga tgaccgttga cgaggagagc gggcgtgatg cggttacgcg
     1561 ctatgttctt ctggaacgtt ataagtttat ggaccatgtg gatatatcgc ttcaaaccgg
     1621 ccgcacccat cagatcaggg tgcatttctc ccacctggga catccggttg tgggagattc
     1681 cgactacggc ggtaacgaca agattttgca gggtttgttc gatcaatacc gaatggacgc
     1741 cagaaaagtg ctggcgatca tgcgccgtca ggcgctccat gcctggcggc ttgaatttcg
     1801 ccatccgcag accggcctga tccacgcagt caagtcagaa ttgcccgatg acataatgaa
     1861 agtcctgtca tacattaaag aaatcgaccc gaaactctga gcgagcaaat ctgaattatg
     1921 gccaaaaaag gaatttatct tgcagaaggt tataaactgg caaaatgctg taatcctgaa
     1981 cccggctcac aaatctgcgg ttattacagc tacaataacg agatggtcgt gcacaagaaa
     2041 ggatgtaaaa atctcgcaaa agtggaaaaa gaaaggctgg ttgacttaaa atggaaggag
     2101 attcttgccg ctgctccgga aatgcccgag ggtgattttg ccaatctcac tgaacttgat
     2161 tttgaggttt tgaaacacca tcgagattat ggaaatgatt attcgctcaa agtggcccgt
     2221 atgcttcata aacccaaaca ggacattttc gacagccatg ccaaattgag ggagatgaat
     2281 ctcctggaac gggtcaagcc cttgatcatt caatatagaa aaggtgttgt ggataataaa
     2341 tggatcaaac atcgcaatca cacgtattat gatttgactg aaaagggaaa gttatattta
     2401 agccactatt tgcacgaata aaacgaacca gaataagaat cacaactttg gagatcagat
     2461 gagagaaaga acaaaagtct taagactggc ggcggcctgt gttttaacgc tgctgttcgc
     2521 gtccccgaca tttgctgcaa ctgaaagcga gcttcttgcc actctgcagg aagagctgct
     2581 ttataatttt gacaaactga aaaatgcgga cactgtcccg ctttattatt tatcctacca
     2641 ggtcatcgag agccaggaat ataatgtttc ggccgaactg ggagcgcttg aaagcgagga
     2701 tgattcacat gtacgtgacc ttgatgtaat cgcacgtgtg ggatcatacg agctggacaa
     2761 ttcgcgcgag gtccccggtg cgggaggagg cctctcgacc ggcgcggtcg atctgccgct
     2821 gggaaacgat cctgtcgcct tgaagatgaa gatctgggag gaaaccgacc gcaggtacaa
     2881 ggaagtccag aagcaataca ccaatgtgat taccggcacc actgtcaaag tggaggccga
     2941 ggatcagtcg gatgatttct cccgcgagga gccgtccata tatatcggcg atgtcgccgg
     3001 gctggattat gacaggcaga tatgggaaga caaactggta cgatacagtt caatattcag
     3061 caactatccc gaaatcgaaa actcctcggt cagtctttcg gccaggaccc gcaacagtta
     3121 tctggttacg acagaaggca caaaaatcca ggacggccag agttatatcc gcctcaacat
     3181 catgtgcagc agcactgccg atgatggcat ggttctaagg cgatttgaag gtttcgatgc
     3241 ggccgatttc agcaacatgc cggatgatga aaccgttatc accgctatca acaggctgat
     3301 cgatgaattg cttgccctga aaagcgctcc gcttgcggag ccatataccg gcccggcaat
     3361 tctgaccggc cgggccagcg gggttttctt ccatgagatc ttcggccacc gcatggaggg
     3421 acacaggcag aagagcgaat ctgagggcaa aaccttcacc gacaaagtgg gcgaatcggt
     3481 acttccggaa ttcatcagcg tcttcagtga tcctacaatg cggactttca acggaatcga
     3541 tctccgcggt cattacaaat atgacgacca gggtatcgag gcccgcaggg ttactcttgt
     3601 ggataagggt atcctggaga ctttcatgct ctcccgcacc ccgatcgaaa actttcccca
     3661 ttccaacggg cacggcagga aatatgtcgg attcgccccc attgcgcgcc aggcgaattt
     3721 gataattgaa tctgaaaaga gtgtaggatt tgacgagctg aaagagatgc tgatcgagga
     3781 atgcaagctg cagggtaagc cttatggtct gatcttccag gatatatccg ggggattcac
     3841 ttttacaggc cgatttcttc cccaggcatt caaagttata ccgctgatgg tttacaaggt
     3901 ctatgtcgag gacgggcatg aggagctgat acgcggcgtc gatatcgtgg gcactcccct
     3961 gaccagtttc agcaagatca tgattgcggg gaatgatccc gacgtcttca acggcacctg
     4021 cggcgctgaa tcgggatggg tgccggtgtc ggcaatttcg cccagcctgc tactttcaga
     4081 aattgaaatc gagcgcagcc agaaggggca ggaaaggccg ccgcttcttc cctcaccctt
     4141 aaccaaacag cagtaatggc tgaataaagg agaagacgat gaaatcgatt atacataaat
     4201 ttatgctgac actcctttcc gttgcattag tcctgataag ttcacaagca ttgattgcac
     4261 aggatgatcc gttgatacgg gccctgagtg acgaaatgga gcgagcaacc ggcagcctgc
     4321 gtctcgaaga cgaagagccg ccctatttcc ttggttacct cgcaaacctc gaggataatt
     4381 gcaggatacg cgccgagtac ggttcggtga tcgaatgtga ctgccagaag gccaaccaga
     4441 tgttcctcga tttgcgcgtg ggcgattacg aattcgacaa cactaatgta atgaccagtt
     4501 tttttggggt gggaatggcc atggccaatc tgccggtaga aattgtctat gcgccgcttc
     4561 gaaaggatgc ctggctgcag atcgacgaag cttacaagag ggcggttgag acgattgccc
     4621 gaaaacgggc gatgcttcag tctattattc cggatgatac ggtagccgac ctgtcaaaag
     4681 ccgaagtcgt catcgatatg aaaccggatc tctattccag agtcgatacc acggaatgga
     4741 aagagatact ggccgagatg tcggcagttt tcaagaacta tcctgagttc acacgttcat
     4801 cggtacagct caacaccaaa accgagaacc gttatgtcct gaactctgag ggtacgcggg
     4861 tcaggcgcgg gcggcacgtg cactacatct ttatcgaagc cagcaccctg gataaaaacg
     4921 gcattcccgt ttatcaatat gatagaataa ttgtggatga tatagatgat tttccggccg
     4981 ggaatgaatt ggttgagtgg gccgatgatt ttgcgcgcgc ctcggccgca atggttgacg
     5041 cggaaacgac tgacacttat atcgggccgg tacttttcac tccccgcgcc agcgcgcagc
     5101 tgttgagcca gcttcttgtc gataatattt ccaatccgcg caaaccgctg accatggata
     5161 atcgcttcga tcaatatctt tcggatgccc gcctggtcag aaaactcaat ttccgcataa
     5221 tgccggaatt catcagtatt gtggatgatc ctactatcag acagtacaat ggcctgaagc
     5281 tcaagggata ctacgaattt gacgatcagg gtgtggccgc gcagagaata accctggtgg
     5341 aagagggaaa actccgaaat tactacatga gccgtacacc aaccgaaaaa atagagaatt
     5401 ccaacggtca cggccggctg atccaaagca ggatcggagc tgtcgaagtt gtgggcaagc
     5461 ccggcaatgt tattatgcag gcggccgaga cctaccaacc ggaagacttg aaagagcaga
     5521 tgttggaatt gtgccgcgag atgggccttg aatatggttt gattatcgat cggatgaatt
     5581 tctcgggccg cagcaacccc gacgagggca tgagattcag gatgaccggg gaaagcggca
     5641 tccccagggt gatagtcgcc tacctggttt ctgccgagga cggcagtatc actcctgtcc
     5701 gcagcctcga attcgacaac atcaacgaac gggtattgaa ggacatcctg gcggtcggaa
     5761 acgatttcga ggaagctacg attatgtttg acgccgcttt caataacctg gcttcaattg
     5821 tggtgccttc catcctgttc gaggaaatgg aattacagaa agcttctctg caagccaaga
     5881 agccgccgat tgtattgaac ccgctggaaa gagaataagc gattttgggc tggaataaaa
     5941 ttattccagc
//
LOCUS       DSEB01000377            2673 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1005314, whole genome shotgun sequence.
ACCESSION   DSEB01000377 DSEB01000000
VERSION     DSEB01000377.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1005314"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            197..403
                     /locus_tag="ENO07_06760"
     CDS             197..403
                     /locus_tag="ENO07_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01704.1"
                     /translation="MALDESTDGLTKLESNGINVYIDPRLKEHLGKFGDINVDFVTNP
                     MGQSGYMIKIGSEDCSEGGCSGCG"
     gene            complement(502..1554)
                     /locus_tag="ENO07_06765"
     CDS             complement(502..1554)
                     /locus_tag="ENO07_06765"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HDS01705.1"
                     /translation="MGTFENIKKLLGEDESLLDYKCKGIPRDRLHLPGPDFVDRVVYN
                     SDRPVNVMKNLAWIFGHGRLAGTGYLSILPVDQGIEHSGGASFAPNIDYFDPENIIKL
                     AIEGGCNAVVSTFGVLGSVARKYAHKIPFILKINHNELLTYPNKYDQVPFTNIERAYE
                     LGAAGIGATIYFGAQESMRQLQEVSELFSQAHDLGMFTVLWAYLRNSDFKADKDYHTA
                     ADLTGQANHIGVTIEADIIKQKQAQTNGGYKALKFGKTHDLVYDKLTSDNPIDLCRYQ
                     VANCYMGRVGMINSGGASGENDLAQAVRTAVINKRAGGMGMISGRKSFQKPREKGIEL
                     LNSIQDVYLTKEVTIA"
     gene            1620..>2673
                     /locus_tag="ENO07_06770"
     CDS             1620..>2673
                     /locus_tag="ENO07_06770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDS01706.1"
                     /translation="MVNSYMHIQGPATSPLKHKLVISPRFRKSYLYLILCLAIRTHLG
                     IFFPMFIENLKLTQFRNLKKAEIQFSSKNNLIVGPNGSGKTNILEALFYIGSGRSFRT
                     HLDENLANTDSEFFRIEARGKIGRHEVQIEIGVEPGKRKIIKVNSAPIRKLGELFEYF
                     RLVEFSPYDLEIIIGSPSVRRRYISITIAQLDPPHVSVLSDYHKTLAQRNALLKSFEE
                     TPRLSASQEASLAVWDEKLAIHAVQIHQNRKKYTKKIAELSADFYRRISGSEEYLKIE
                     YNPSPGLDEFTADHFCEKLASRRQREIALGQTMYGPHRDDLKFYIDSLEAKNAASQGQ
                     IKTAVLSLKLAQYEYLK"
BASE COUNT          723 a          670 c          611 g          669 t
ORIGIN      
        1 gaataagttg acaatattaa cattgtgaaa aaagtagcat tgtttttata gaaatgagga
       61 gggtgaattc caaatgctga atataacaga taaggcttcc agcgaactta tcaaagctat
      121 ggagtctccc aaagccagag gaaagaatct gattctctac tacatgggcg ccggttgaag
      181 cggcccccaa ttgggaatgg ctctggatga gtctacagat ggtttaacga aattggaatc
      241 caacggaatc aatgtctaca tcgatcctcg tttgaaggag catctcggca aattcggcga
      301 tatcaatgtt gattttgtga caaatccgat gggtcaaagc ggatatatga taaaaatagg
      361 cagcgaagat tgcagcgaag gcggatgttc cggctgcggt tagactgaga acacatatcc
      421 tggaaataaa taagcccctt cgaatattag aaggggcttc ttcttttaag cattacggaa
      481 atacgaagtt gattgttgaa tttaagctat cgtcacttcc ttggtgagat agacatcctg
      541 aattgaattc agaagctcaa ttcccttctc acgcggcttc tggaacgatt tcctgcccga
      601 aatcatcccc ataccgccgg cgcgcttatt aatcacagcc gtcctgaccg cctgcgccag
      661 gtcgttttct cctgaggctc cgcccgagtt gatcatcccg acccggccca tatagcagtt
      721 ggccacctgg tagcggcaga ggtcgatggg attatcgctt gtgagcttat catagacaag
      781 gtcatgagtc ttgccgaatt tcagggcctt gtagccgcca ttggtctggg cctgcttctg
      841 cttgatgata tcagcttcaa ttgtgacacc gatgtggttg gcctgcccgg tgaggtccgc
      901 ggctgtatgg taatctttgt cggccttaaa atcggaattt ctcagatagg cccagagcac
      961 cgtaaacata cccaggtcat gtgcctggct gaagagttct gagacctcct ggagctgacg
     1021 catcgactcc tgcgcgccga aatagatggt ggcaccgatg cccgccgcac cgagctcata
     1081 ggcccgctca atgttcgtaa acggcacctg gtcatattta ttcgggtagg ttagaagctc
     1141 attgtgattt atcttgagaa tgaagggtat tttgtgagca tatttgcgcg ccaccgaacc
     1201 aaggacgccg aatgttgata cgaccgcatt acatcccccc tctatcgcca gcttgataat
     1261 attttcggga tcgaaatagt cgatattggg cgcaaaggat gctccgccgg aatgctcgat
     1321 tccctggtca acaggcagaa tcgaaagata tcccgtaccc gccagacggc cgtggccgaa
     1381 aatccacgcc agattcttca tcacattgac aggccgatcg gaattataca caaccctgtc
     1441 gacaaaatca ggtcccggca gatgcaaacg atccctggga atacccttgc atttgtaatc
     1501 cagcaaggac tcatcttccc ccagaagttt ttttatgttc tcaaaggttc ccacgactga
     1561 ctcctttcag atttcatttt caaaaaatat aatgatgtaa tttatccaat atcactgtaa
     1621 tggtcaattc atatatgcat atacaggggc ccgcaacatc ccctttgaag cacaagttag
     1681 tgatttcgcc ccgatttcgc aagtcatatc tctatctgat cttatgcttg gcaatccgca
     1741 ctcatttggg tatattcttc ccgatgttta tcgagaatct aaaactgacc cagttccgaa
     1801 atcttaaaaa ggccgagatc caattctcat ccaaaaacaa tctgattgtc gggcccaacg
     1861 gctcgggcaa aaccaatata ctagaggcgc tcttttatat cggctcggga cgctcgttcc
     1921 ggactcatct ggatgagaac ctggccaaca ccgattctga attcttccgc atcgaggccc
     1981 ggggaaaaat cggccggcat gaggttcaaa tagagatcgg agtcgagccc ggcaaaagga
     2041 aaattatcaa ggtcaactcc gcacctatac gcaaactcgg cgaactattc gaatacttcc
     2101 gcctggtcga attctcaccc tacgatctcg agataataat cggctctccc tctgtcagac
     2161 gaagatatat ttccatcact atcgcccagc ttgaccctcc gcatgtctcg gttctgtcgg
     2221 attaccacaa aaccctggca cagcgtaatg cgctcctgaa atcatttgaa gaaactcccc
     2281 ggttgtcagc atcccaggaa gcctcgctgg ctgtgtggga tgagaaactt gcaattcacg
     2341 cagtacagat tcatcaaaac cggaaaaaat atacaaaaaa aattgcagaa ctctcagccg
     2401 acttctatcg cagaatctcg ggctcggagg aatatctcaa aatagaatac aatccctctc
     2461 ccggtttgga tgaatttacc gccgatcact tttgtgaaaa gctggcctcc cgaagacagc
     2521 gcgagatcgc tctgggacag acgatgtacg gtccgcacag ggatgacctg aagttttaca
     2581 tcgacagtct tgaggccaaa aatgcggcct cgcagggaca gataaagact gccgtattga
     2641 gtctcaagct ggcccagtat gagtatctta aac
//
LOCUS       DSEB01000378            5006 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001309, whole genome shotgun sequence.
ACCESSION   DSEB01000378 DSEB01000000
VERSION     DSEB01000378.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001309"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..597)
                     /locus_tag="ENO07_06775"
     CDS             complement(<1..597)
                     /locus_tag="ENO07_06775"
                     /inference="COORDINATES: protein motif:HMM:PF01578.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01707.1"
                     /translation="MRDLDFLKIFLGILFIAVIVLSFVTGKPVRGYGDEFRIFYYHVP
                     CAIVSFIAFLVNLIYSMKYLRSRYYLDDMKAASAAEIGIVFGVLATLTGAIFAKITWG
                     TYWNWDIRQTSIVVLLAVYGAYFALRTAVEPEERRSAFSAVYAILAFIAVPTFGFVVP
                     RLYDSLHPKDTLVSGGKIALGGTVAVVFFGSLIAFTILF"
     gene            complement(643..1338)
                     /locus_tag="ENO07_06780"
     CDS             complement(643..1338)
                     /locus_tag="ENO07_06780"
                     /inference="COORDINATES: protein motif:HMM:PF03379.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmB"
                     /protein_id="HDS01708.1"
                     /translation="MKSRSQMSELTWPAKLIALVVKDFRVELRTRYSLNALIMFALVT
                     LFALGVTFTGQNLTFRIHAALIWIMIYFAGLQGLSGGFVKEEESRTAMALRLYAPSSV
                     IFFGKLIFSLLILTILVVFLFPLYYILMNLEITRIWIMLLVLILGIFGLSATSTITAA
                     MVSKANVKGALFAVLSFPLMLPLLLVAISATTVCLGGGDSQALWDSLKIMIAYPVIVT
                     AVSFLLFDYVWNN"
     gene            complement(1295..1912)
                     /gene="ccmA"
                     /locus_tag="ENO07_06785"
     CDS             complement(1295..1912)
                     /gene="ccmA"
                     /locus_tag="ENO07_06785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HDS01709.1"
                     /translation="MIIISAENLSMRFGLRGVFKDISFRLASPSSLTVTGPNGSGKST
                     LLKIIAGLLVPTSGRVRMESDSDEIKAESRRKLMAMVSPEMNFYDELTGAENLEFFMK
                     ASGSRLKPEEIEEALNEVGLQGRGDDYLKAYSSGMRMRLKYALAILKQPEIMIVDEPS
                     TNLDASGRKIIYDLMHRHQQNGILIFATNEDDEIAIANERINLAR"
     gene            complement(2010..2813)
                     /gene="lepB"
                     /locus_tag="ENO07_06790"
     CDS             complement(2010..2813)
                     /gene="lepB"
                     /locus_tag="ENO07_06790"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDS01710.1"
                     /translation="MKVREKESKKSPEKSSKQDGYQVTLEYIKSFLIALLLALLIKAT
                     VIEAYKIPTPSMEKTLLAGDFILGNKFIYGIKIPFTDIRLPAIREPKRGDIVIFRPPH
                     SPHENYVKRLIGMPGDTLKITRKRVYINGELYNDTAFTQFNSDYMMPRDRVLGPDPYV
                     QAGIPNNGRRPWRPFRDNSYQIVVPEGKYFMMGDNRDNSLDSRMWGFVDREQILGKAL
                     IIHWSWDIHDTGAPAVEWRNPLSIIRNIIYNLIHFPERVLWERLGKIPQ"
     gene            complement(2860..3930)
                     /gene="rseP"
                     /locus_tag="ENO07_06795"
     CDS             complement(2860..3930)
                     /gene="rseP"
                     /locus_tag="ENO07_06795"
                     /inference="COORDINATES: protein motif:HMM:PF02163.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDS01711.1"
                     /translation="MIVTILAFIFVLGVVVFFHELGHFLMAKASGVRVYKFSLGFPPK
                     MIGFKKGDTEYCISWIPLGGYVKMAGENPVEGEELGDDPGSLMNKPAWKKALIFAAGP
                     LANYFTAIIIATGLFFFLGKEQVDPERVVIGLIEENSPAAELGLKPGDVILSIAGNDV
                     NQLRDIHEVVVDSPNQEFNIVWERNGQTMSAMIETAADTIPNYQGEMVARGKIGIAQA
                     TDTIPVGVGEAFILANRASWRVTVLMADIVQKLVTGRASPKILGGPIMIAKVSGEKAR
                     EGFVSLLDLVVLISINLAILNLLPIPILDGGHLLLLLIETVRRKPLTLKQKAIFQQVG
                     LAILLALMVMVFYNDIFNNLMG"
     gene            complement(4098..4712)
                     /locus_tag="ENO07_06800"
     CDS             complement(4098..4712)
                     /locus_tag="ENO07_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDS01712.1"
                     /translation="MVTIERNKLEAQKYEQKINVSSDGGDTTIILTAEKVPNPVVLME
                     TSMGDIKLELFPEVAPIHVQNFLNLTRSGFYDSLIFHRVIDGFMIQAGGYTDELELKP
                     ARNIPAEFNDSLHREGTLAMARRDDPNSASSQFYICLAPRPGLDGRYTVFGKTIEGLD
                     VVHEIGKVETNKGENRDAGLMPNQPLEPVYIEKMNILVDYEPDE"
BASE COUNT         1366 a         1304 c         1085 g         1251 t
ORIGIN      
        1 gaacaatatt gtaaatgcga tcaatgatcc aaaaaatacc accgccacag tcccgcccaa
       61 agctatcttg cctcccgata ccagtgtatc cttgggatgg agcgaatcat acagcctggg
      121 aaccacaaat ccgaaggtag gcacggctat gaatgccaat atggcataga cagccgaaaa
      181 ggccgagcgt ctttcctccg gttcaaccgc cgtgcgtaag gcgaaatagg cgccatagac
      241 ggccagcaga acgacgatcg aggtctggcg tatatcccag ttccagtatg ttccccaggt
      301 gattttggcg aaaattgcac cggtcaatgt cgccagcaca ccgaagacaa tcccgatctc
      361 ggccgcgctg gcagccttca tatcgtcgag ataatagcgg cttctcagat atttcataga
      421 atagataaga ttcaccagaa atgcgataaa gctgactatg gcacagggaa catgatagta
      481 gaatattctg aactcatcgc catagccgcg taccggtttg ccggttacga atgaaagcac
      541 tataacggca ataaatagaa ttccgagaaa tatttttaaa aaatcgaggt ctctcataag
      601 tccttggtct ttaagctata agaataataa tcatctcttt tatcagttgt tccaaacata
      661 atcgaaaaga agaaaagaaa ccgcagtcac aatcaccggg tatgctatca tgatcttcaa
      721 actgtcccac aaagcctgac tgtccccgcc tcccaaacac actgtggtgg ccgagatcgc
      781 aaccagaaga agcggcaaca tgagcgggaa cgatagaact gcaaacagcg cccccttgac
      841 attggccttt gaaaccatgg ccgcggtgat cgttgatgtc gcactcaagc cgaatatacc
      901 caaaattaga accaaaagca tgatccagat tcgtgtaatc tccagattca tcaatatata
      961 ataaagcggg aataaaaaca caacaagaat tgttaggatt agcaggctga atatcagctt
     1021 accgaaaaaa atcacgcttg atggcgcgta aagtctcagg gccatcgcgg ttctggattc
     1081 ctcctctttc acaaatcctc ctgaaagacc ctgcagcccg gcaaaatata tcatgatcca
     1141 gatcagcgcg gcatgaatcc ggaatgtcag gttctggccg gtaaaggtca ctccaagggc
     1201 gaacaaagta accagtgcaa acataatcag cgcattcagt gaatagcgtg tacgaagctc
     1261 caccctgaag tccttgacga ccagggcgat cagcttagcg ggccaggtta attcgctcat
     1321 ttgcgatcgc gatttcatcg tcctcgtttg ttgcaaatat caatatccca ttttgctgat
     1381 gccggtgcat caaatcgtaa attattttcc ttccgctggc gtcgagattg gtggagggtt
     1441 cgtcgactat cattatttcc ggttgcttca ggatagccaa agcatatttc aggcgcatcc
     1501 gcatgccgga tgaatatgcc ttcaaataat catcccccct gccctgcaaa ccgacctcat
     1561 tcagggcctc ttcaatttct tcaggtttca gcctggagcc ggaagccttc atgaagaatt
     1621 ccaggttctc ggccccggtc agttcatcat agaaattcat ctcgggcgaa accatggcca
     1681 taagcttcct ccggctttcg gcctttatct catctgaatc cgattccata cggaccctgc
     1741 cggaggtggg aactaataat ccggcaatta tttttaatag agttgattta cccgagccgt
     1801 tgggaccggt aactgttaag gatgaagggg aggcgagcct gaaactgata tctttgaaca
     1861 ccccccgcag gccaaaacgc attgaaagat tttctgctga aataataatc atgatcttta
     1921 atgcgaatga aaatactcca atatggacag acagtcaaat aaaaactggt tcaataggat
     1981 tgatgattag agcttaaagg tgggggcctc tattgcggga ttttgcccag cctttcccac
     2041 aagactcttt ccgggaaatg aatcaggtta taaataatat ttcgaattat gctgagagga
     2101 tttctccact cgaccgccgg ggcacctgtg tcatgaatat cccaggacca gtgaataatc
     2161 agcgccttgc ccagaatctg ttcccggtcc acgaagcccc acatacgcga atcaagcgag
     2221 ttatccctgt tatcgcccat catgaaatat ttcccctcgg gtactacaat ctgatatgag
     2281 ttgtcccgga agggacgcca gggcctgcgg ccgttattgg gaatgccggc ttgaacatac
     2341 ggatccggcc cgagcactct gtcacgcggc atcatatagt cactgttgaa ctgggtaaat
     2401 gcggtatcgt tgtataattc accatttata taaacacgtt tcctggttat cttgagcgta
     2461 tcgcccggca tgccgatcag ccttttgaca taattctcat gggggctgtg aggcggccga
     2521 aatatcacga tatctcccct ctttggctcg cggatagcgg gcagacggat atcggtaaag
     2581 ggtattttta tgccataaat gaacttattt cccagtataa aatctcccgc cagcagggtt
     2641 ttttccattg agggcgtagg aattttataa gcctcaatta ccgtggcctt aatcaaaagg
     2701 gccagaagca gggcaattaa aaaagatttg atgtactcca gggttacctg atagccatcc
     2761 tgtttgctgc tcttctcagg agacttttta ctttcctttt ctcttacctt cataagctcg
     2821 ccagttcgtt aaaaaaaatt tgatcgggaa taaagaaacc taccccatca aattattgaa
     2881 tatatcattg taaaatacca ttaccatcag agccagcagg attgccaggc ccacctgctg
     2941 aaagatagcc ttctgtttca gagtcagggg cttcctgcgt acagtctcga tcaagagcag
     3001 gagcagatgg cccccgtcga gaatgggtat aggcagcagg ttaagtattg ccaggtttat
     3061 ggatatcaaa actacaaggt ccaagaggct aacaaagcct tcgcgcgcct tttctccgga
     3121 gaccttggcg atcatgatcg gcccgcccag aatcttaggt gaagcccgac cggtcacaag
     3181 cttttggaca atatccgcca tcaaaacagt aacccgccag gaagctctat tggccaggat
     3241 gaaggcctca ccgacgccga ccggaatcgt gtcggtagcc tgagcaatcc cgattttacc
     3301 ccgcgcaacc atttcgccct gataatttgg aatggtatcc gcggcggttt caatcatggc
     3361 cgacatcgtc tgaccattac gctcccatac tatattgaat tcctggttgg ggctgtcgac
     3421 aaccacctca tggatatccc gaagctgatt tacgtcattt ccagcaatag acaggatcac
     3481 atcgcccggt ttgagtccca gctctgcggc aggagaattc tcttcaatca ggccgataac
     3541 cacacgctca ggatcgacct gctctttccc gaggaagaag aacaacccgg tggcgattat
     3601 aatcgcggta aagtaattag ccagcggccc ggctgcaaaa atcagcgcct ttttccaggc
     3661 cggcttattc atcaggctgc cgggatcatc cccaagctct tccccctcaa caggattctc
     3721 cccggccatc ttgacatagc cgcccagcgg aatccaggaa atgcagtatt ccgtatcacc
     3781 cttcttgaat ccgatcatct tgggaggaaa gcccagtgag aatttatata ctctaacccc
     3841 ggaggccttg gccatgagga aatgtcccag ttcgtggaaa aaaactacca ctcccaggac
     3901 aaaaataaac gcaagtatcg ttacgatcaa ttaaggtatc ctcgaatcat caaatcatct
     3961 ccacacgtca gcttaccctt atcatatcaa tttattcaag cagtcaataa ttatatacga
     4021 tattgtcaac caaaagatta aaccatcgct ttactatttt gttcacattt aatttatcgc
     4081 cggaattccc ggctatctta ttcatcaggc tcataatcaa ccagaatatt catcttctcg
     4141 atataaaccg gctccagtgg ctgattcggc attaatcccg catcccggtt ttcacctttg
     4201 ttcgtttcca cttttccgat ttcatgtacg acatcaagcc cctcgatcgt tttcccgaaa
     4261 actgtatagc gtccgtccag tcccgggcga ggcgcgagac agatatagaa ctgggagctg
     4321 gcgctgttag gatcatcacg tcgggccatt gcaagcgtgc cctcgcgatg caggctgtcg
     4381 ttgaactcgg ccggaatgtt cctggccggt ttaagctcca gctcatctgt atatcccccc
     4441 gcctgaatca tgaaaccgtc tatcacccga tgaaagatca ggctgtcata aaatccagat
     4501 ctcgtcagat tcaggaaatt ctgcacgtga atcggcgcaa cttccggaaa aagctccagc
     4561 ttgatatccc ccatggaggt ctccatcaaa actaccggat tggggacttt ctctgcagtc
     4621 agaataatcg ttgtatctcc gccgtcggag gagacattga tcttctgttc gtatttttga
     4681 gcctccagct tgttacgctc tatcgtgacc atcaccagaa ttgtgtcatc accggttcgg
     4741 cccccaaatg gcttgacatg aatccactcg gcatcatcgg taatgcgcca gttcagctta
     4801 tcaccgccgg aattggtcaa cttgaatgtt tcctggcttc tcgttcttcc catatcgatt
     4861 ttatcctttg agacatacaa ctccggcccc ccgcaacccg aaagaacggc aaccagaaac
     4921 aaaatgacaa acggcttgag atattgcata ttgatcagga ccctcctgct taaattcaag
     4981 agcttaataa taatacagac atgtaa
//
LOCUS       DSEB01000379            3576 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002785, whole genome shotgun sequence.
ACCESSION   DSEB01000379 DSEB01000000
VERSION     DSEB01000379.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002785"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..716)
                     /locus_tag="ENO07_06805"
     CDS             complement(<1..716)
                     /locus_tag="ENO07_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018214498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase, dissimilatory-type subunit
                     alpha"
                     /protein_id="HDS01713.1"
                     /translation="MADQLPNTPMLDELEKGKWPSFVKEIKTAAAKNDMSKDLLGILE
                     KSYNEKIGHWKHGGIVGVLGYGGGVIGRYCDVPEEFPGVSHFHTMRINMPAAWFYTSE
                     ALRTIADIWERHGSGLTNMHGSTGDIILLGTTTDELEPTFQELAQAGFDIGGSGSDLR
                     TPSCCNGMARCEWACYDTMKACYHMTMHFQDELHRPAFPYKYKFKFAGCPNDCVASIA
                     RADMSFIGVWRDEIQIDQDKV"
     gene            complement(1218..1424)
                     /locus_tag="ENO07_06810"
     CDS             complement(1218..1424)
                     /locus_tag="ENO07_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01714.1"
                     /translation="MAKHEKNKGVFVCKGRRVHVVYNDITLHFDFDEFEKFTFMLDDA
                     YHRIKGELKDDSDTDDISDDNPEE"
     gene            1648..2913
                     /locus_tag="ENO07_06815"
     CDS             1648..2913
                     /locus_tag="ENO07_06815"
                     /inference="COORDINATES: protein motif:HMM:PF13372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01715.1"
                     /translation="MRPQKILLSIALFILALMLIPSFLNAQGIKVADGLTIDGQIRER
                     TEFDGKDFNTDTDMIERSYLRTRLGLKFTRIENTQIYLQFQDSRNLGTNSGGLTNDDN
                     LGIHQAYIKFMIPGMEGSWLQLGRFEAKYGRERVIGAVGWSNIGRTFDGLRFGYDGGF
                     LSFDLFLLKIVERGFGTPSRAGDHHLYGLYTRWLERHLHIFGLLDYDHQESFPGSDEP
                     TLARYTLGTYYHRTTRSGFDVALDLAYQGGEYDRINVDKIAAYMLAAELGYTFVNSDA
                     RPRIGAGVDMTSGDEGIDASELNTYDNLYYTGHAYRGYMDLFVENPFEGLFDLFGRVS
                     IMPTDKWWFGFDFHYFRTMEDYFILTEPIGDNTSSAIGSEIDFTAKYNIYENLDAQGG
                     LSLFFPSEDWQGDDSDTGTWFYFMLTAGL"
     gene            3469..>3576
                     /locus_tag="ENO07_06820"
     CDS             3469..>3576
                     /locus_tag="ENO07_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004346229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDS01716.1"
                     /translation="MNIQPLADRVLVEPMEPAEVKKGGIIIPDTAKERPQ"
BASE COUNT          951 a          843 c          816 g          966 t
ORIGIN      
        1 accttgtcct ggtcgatctg gatctcatct ctccagacgc cgataaagga catgtccgca
       61 cgggctatgg aagccacgca gtcattggga cagccggcaa atttaaattt atacttatag
      121 gggaatgccg ggcggtgaag ctcatcctgg aaatgcatgg tcatatggta acaggccttc
      181 atggtatcat aacaggccca ttcacatctg gccataccgt tgcaacagct cggggttctc
      241 aggtcggaac cggaaccgcc gatatcgaac ccggcctgag cgagttcctg gaaggtcggt
      301 tcgagctcgt cggtagtcgt tccgagaaga atgatatcgc cggtcgatcc atgcatgttg
      361 gtcaggcccg atccatgtct ctcccagata tcagcaatgg ttctcaacgc ttccgaggta
      421 taaaaccagg ccgcgggcat gttgatacgc atggtatgga agtgcgagac tcccggaaat
      481 tcttcgggca catcacaata acggccgata accccgccgc cataacccag aacaccaact
      541 atgccgccgt gcttccagtg gcctatcttt tcgttatagg acttctccag aatgccaagc
      601 aagtccttgg acatatcatt tttggctgca gcagtcttga tctccttaac gaaacttggc
      661 catttaccct tctcaagttc atccagcatc ggggtatttg gtaattggtc tgccataatg
      721 actccttact tttactctgc gaggcaaaag cctcttgtat ttgatctgca aatatctcta
      781 cttttgttct gccatctcgt acttggtgaa atttataaca aactaacgta tgattattta
      841 tttttacgtc tttccattgc cggacgagaa aatctcttca ataatgtctt cccgatatgc
      901 aaactcgact gtattttcct gcacttcaaa tgtattaaaa gcaattaatg taaaataatt
      961 cacaagcatg tcaagcccaa aatgcgcaat tattcgttta taaacaatta tttatgctgc
     1021 ttttacgaac tgctttcctt atgcattcca ttactattga tacgaagtaa aacgccccgc
     1081 ttttaactaa atattggtat tccgatggat aaataatata ggctaatcta acatttttag
     1141 agcagccaag cttgtgaaaa aagtatctaa gtaaatagga taaatggaat ctcaggattc
     1201 ttcgaagagg cgctcattca ttcttcggga ttgtcatccg atatatcatc cgtatcggaa
     1261 tcgtctttga gctccccttt gattcgatga taagcatcat ccagcataaa tgtgaatttt
     1321 tcgaattcat caaaatcgaa atgcagggta atatcgttat agacgacatg cacccgcctg
     1381 cccttacaga caaagacccc tttgtttttc tcatgctttg ccatcttgcc ctcgctgatt
     1441 gatgatctgt tctattatac gtaatatggc tcccagtgtc aggctatttc tggacatact
     1501 attcaccatc cgtaccaggc aaaaccttct tgttgacttt atgctcccta tttatttttt
     1561 tgacatgtat caattacaaa cgtcttggaa aatgacacct tgggtagaat attaagaaaa
     1621 atcaaataag caaaaagtga gggaacgatg agaccacaga aaatcttgct cagcattgcg
     1681 ttatttattc tggcgctgat gcttatcccg tcatttctga atgcccaggg gatcaaggtc
     1741 gccgacgggc tgacgattga cggccagatc cgtgaacgta ccgagtttga cggcaaagat
     1801 ttcaacactg acaccgacat gatcgaaaga tcatacctga gaacacgtct gggactgaaa
     1861 ttcacccgca ttgaaaatac ccagatctac ctgcagtttc aggattcgcg caatctgggc
     1921 acaaactcag gcggccttac caacgacgac aacctgggca ttcaccaggc ctatatcaag
     1981 ttcatgattc cgggcatgga gggtagctgg ctgcagctcg gacgttttga agccaaatat
     2041 ggacgtgaaa gggtaatcgg cgctgttggt tggagcaata tcgggcggac attcgacggc
     2101 ctgcgttttg gatatgacgg tggatttctg tcatttgatc tgttcctgct caaaatcgta
     2161 gaacgcggat tcggcacacc atcgcgtgcc ggggatcatc atctttatgg cctctacacc
     2221 cgatggctgg aaaggcacct gcatatattc gggctgctgg attacgacca ccaggaatcc
     2281 ttccccggtt cggatgagcc taccctggca cgatatacac tggggacata ttatcatcgt
     2341 accacccgct ccggctttga tgttgcgctc gatctggctt atcagggcgg ggagtatgac
     2401 agaatcaatg tcgataaaat agcggcttac atgcttgcgg cagaacttgg ttatacattt
     2461 gtaaacagcg acgccagacc gcgtatcggg gcaggtgtgg atatgacctc cggcgatgag
     2521 ggcattgatg cctctgagct gaatacctac gataatcttt attacaccgg gcatgcatac
     2581 cggggatata tggatttatt tgttgagaat ccgttcgagg gtctcttcga tcttttcgga
     2641 cgggtgtcta taatgccgac cgataaatgg tggtttggat tcgattttca ttatttccgc
     2701 accatggaag attacttcat attgactgag ccaattggtg ataacacatc cagcgccatc
     2761 ggcagcgaga tcgatttcac ggcgaagtat aatatttatg agaatctcga cgctcagggc
     2821 gggctgtctc tgtttttccc atccgaggac tggcagggtg atgactccga caccggcacc
     2881 tggttttatt tcatgctgac cgcaggattg tagttgaaat attacgcggc gcacgaggac
     2941 gtacgccgca cacaaacgtt gaccgctgga ttgtaaagcg gatgcgggga aaaattgcag
     3001 ggcaggttcc ctgcgaacct gccatcgctg acaaatggat ttgtcggcgg cacccgaatt
     3061 taataggcag gaccacgggg gtcttgcgct acgcaaactg aaataacacg atgtaatttc
     3121 gtccatgtgc cgccgggaac ccattcccgg ccgatagcca tcgggttagg gaacccgacg
     3181 gcacaaaaat acgattagtt ttgtaggggg cttctctacg aactccgaat gacattttgt
     3241 gaacaccgac tttatcaaca agcccaaaag ggatgggata tccataaaac agacatctaa
     3301 tagcatccca aaaaatctca aaaaaactct caattcccac ttgatttttt ctgcggaact
     3361 tttatattgc cccgctgtta gcactcttat taagtgagtg ctaataatgg caagtagata
     3421 aattaagtct aatatattga gtaaattatt aaaaatgagg aggttcgcat gaacattcag
     3481 cctttggcag acagggttct ggttgagcca atggagcccg ccgaggttaa aaagggtgga
     3541 attattattc ccgacaccgc caaggaaagg ccccag
//
LOCUS       DSEB01000380            3171 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003658, whole genome shotgun sequence.
ACCESSION   DSEB01000380 DSEB01000000
VERSION     DSEB01000380.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003658"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..223
                     /locus_tag="ENO07_06825"
     CDS             <1..223
                     /locus_tag="ENO07_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010541184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDS01717.1"
                     /translation="ETLAFASSLSKDAKDRFEDKDKKTDKSFKVGQLIAEMAKAKGVE
                     NVVFDRQGGRYHGRVRAVAEGARKGGLNF"
     gene            235..753
                     /locus_tag="ENO07_06830"
     CDS             235..753
                     /locus_tag="ENO07_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDS01718.1"
                     /translation="MRPQRQRYEQDGFELDEHVIHVNRVAKVVKGGRKFSFTSLVAVG
                     DRNGSVGVGLGKALEVSGAIHKATDAARKDMAKVSMVGGTIPHKIIGKFGAARVLLRP
                     ASPGTGIIAGAGVRAIMQAAGIQDILTKSLGSSNPQNIVKAAMDGLRRLRTEEEALAF
                     REIEPEKKSDAS"
     gene            743..925
                     /locus_tag="ENO07_06835"
     CDS             743..925
                     /locus_tag="ENO07_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019174653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDS01719.1"
                     /translation="MPAKLKVTQVRSLSGRIQKHRRTIEALGLKRIHHSVIHEDTPQI
                     RGMIKAVSFMVKVEEV"
     gene            926..1369
                     /locus_tag="ENO07_06840"
     CDS             926..1369
                     /locus_tag="ENO07_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004798238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDS01720.1"
                     /translation="MELHNLEKPSGSKRDRRRVGRGAGSGQGGQAGRGHKGQRSRSGS
                     KRKYWNEGGQMPLARRIPKRGFVNPNKVYFQLVKIGDIAKKFTEPGEVNPEALKQAGL
                     IKTTAEPIKIMGEGSLEIGLTIYAQAFTKSAAELISKAGGKAEKI"
     gene            1370..2689
                     /gene="secY"
                     /locus_tag="ENO07_06845"
     CDS             1370..2689
                     /gene="secY"
                     /locus_tag="ENO07_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDS01721.1"
                     /translation="MLETFRNVFKIKELRTRILFTLGILIVYRIGGHIPTPGIDGDAL
                     FRFFNQPGGGGTLFGLFDLFVGGAFTKATIFSLGIMPYISASIILQLLGPVVPHLQRL
                     QKEGEEGRRKITQYTRYGTVLIAALQAYGTSVFLQSLNAQTGTMIVTNPGVGFQLLTM
                     LTFTTGTIFIMWLGEKITERGIGNGISLIIFIGIVARIVPGVMGEIQKIVYDPSRIAG
                     AIVIIALMIAVIASVIMVTRAQRRIPVQYAKRVVGRKVYGGQATHLPLSVNTAGVIPI
                     IFAQSIMFAPQTLATFFPGSGIAQFLNTHFNFTSVLYNIIYGVVIVFFAFFYTAIVIN
                     PVQLADNMKSQGGFIPGKRPGKATANYIDKVLTRITLPGAIFFALIAVLPNFLMGYAG
                     VRFYFGGTSVLILVGVALDTLQQIESHLLMRHYDGFIKKGKIRGRSY"
     gene            2691..>3171
                     /locus_tag="ENO07_06850"
     CDS             2691..>3171
                     /locus_tag="ENO07_06850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HDS01722.1"
                     /translation="MLLVMLGPPGSGKGTQASRLAERLDLHHLSTGDMLRSAVKNETE
                     LGKKAKEYMDKGDLVPDSLILDMIRETIKNDQGAGFIFDGFPRTIPQAEGLDRMLQSE
                     SEALDGALNLEVSDQEVIKRLSGRYFCPQCQRTYNYPSNLPKKEGICDECGVELQKRK
                     "
BASE COUNT          772 a          735 c          926 g          738 t
ORIGIN      
        1 agagacactg gcgttcgcat catcgctctc aaaggacgca aaggatagat tcgaagataa
       61 ggacaagaag accgacaagt cattcaaggt cgggcagtta atagccgaaa tggcgaaagc
      121 caagggcgtt gagaatgtgg tctttgaccg ccagggcggc cgttaccatg gccgtgtcag
      181 ggccgtggcc gagggcgccc gcaaaggcgg actgaacttt tagtaaggat tgttatgagg
      241 cctcagagac agagatacga acaggatgga tttgagttag acgagcatgt tattcatgta
      301 aatcgtgtgg ccaaggtcgt gaagggcgga cgcaagttct cctttacctc tcttgtagcc
      361 gttggtgatc gtaacggctc ggtcggtgtg ggattgggca aagctctgga agtttcaggt
      421 gcgatccata aggctaccga tgcggcccgc aaagatatgg ccaaagtctc catggtgggc
      481 ggaaccatcc cgcacaagat catcggcaag ttcggggcgg ccagggtgct cctgcgtccg
      541 gcctcgcccg gtaccggtat tattgccgga gccggagtgc gtgcgattat gcaggccgcc
      601 ggaattcagg atattctgac caaatccctg ggatcgtcaa atccccagaa tatcgtgaaa
      661 gcggccatgg atggactcag gaggcttcgc acagaagaag aggccctggc tttcagggag
      721 atagaaccgg agaagaagtc cgatgccagc taaattgaaa gttacacagg tcaggtcgct
      781 gagcgggcgt atccagaaac acaggcgcac gattgaggcg ctgggtttaa agaggattca
      841 tcatagtgtc attcacgaag acactccgca gatccgcggc atgataaagg cggtttcgtt
      901 catggttaaa gtggaggagg tataaatgga gcttcacaat ctcgagaaac cgtctggttc
      961 gaaacgggac agaagacgcg taggacgggg cgcaggctcc ggacagggcg gccaggctgg
     1021 aaggggccac aagggacagc gctcccgttc aggctccaag cgcaagtact ggaacgaggg
     1081 cggtcagatg ccgctggcac gccgtattcc gaaacgagga tttgtgaatc cgaataaggt
     1141 ttatttccag cttgtaaaga ttggcgatat agcaaaaaaa tttacagagc cgggcgaggt
     1201 caatcccgag gctctgaagc aggctggatt gataaagacc accgcggaac cgatcaagat
     1261 catgggcgag ggatcgctcg agatcggttt gaccatctat gcgcaggcat ttacaaaatc
     1321 agctgcggaa ttgatttcca aggccggtgg aaaggcagag aagatataag tgctggagac
     1381 attcagaaac gtattcaaaa ttaaggaatt gcgaaccagg atcctcttta cgttggggat
     1441 tttgatagtt tatcgtattg gcggccatat acccactccc ggtatcgatg gtgatgcgtt
     1501 gttccgcttc ttcaaccagc ccggcggagg aggcactctg tttggtctgt ttgacctgtt
     1561 tgtgggaggt gccttcacca aagctactat attttcgctg ggtattatgc catatatctc
     1621 ggcctcgatt atattgcagc ttttaggacc ggtggtacct catttacaga gactgcagaa
     1681 agagggcgaa gagggacgac gcaagattac ccagtatacc cgttacggta cggtcttgat
     1741 cgccgccctt caggcttatg gaacatcagt gttcctgcag tcgcttaacg ctcaaaccgg
     1801 tacaatgatc gttaccaatc ccggcgtcgg attccagctt ctgaccatgc tgaccttcac
     1861 caccggaacg atttttatca tgtggctggg tgagaagatt accgagcgcg gaatcggaaa
     1921 cggcatctct ctcatcatct tcatcggaat cgtggccagg attgtgccgg gagtgatggg
     1981 cgagattcag aagattgttt acgatccgag ccgtattgcc ggcgcgatag taattatcgc
     2041 cctgatgatc gctgtcattg cttcggtcat catggttacc agggcccagc gcagaattcc
     2101 tgtgcagtat gccaagagag tggttggaag aaaagtttac ggcggtcagg cgacacattt
     2161 gccgttgtcg gtcaacaccg ccggtgttat cccgattatt ttcgcgcagt cgattatgtt
     2221 tgccccgcag accctggcga cattctttcc cggttccgga attgcgcagt ttttaaatac
     2281 gcactttaat ttcacatccg ttctttataa tataatttat ggtgttgtaa tcgtattctt
     2341 tgccttcttc tacacagcga tcgtgattaa cccggtacag ctggctgaca acatgaagag
     2401 ccagggcggg tttattccgg gtaaacgtcc cggcaaggcc acggccaatt atatcgataa
     2461 ggttttaacc cgaataactc tgccgggcgc tatcttcttt gccctgatag cggtactgcc
     2521 gaatttcttg atgggatacg cgggagtgcg gttttatttc gggggcacct ctgtgctgat
     2581 tttggtcggc gtggccctgg atacgctgca gcagatcgag tcgcatctgc tgatgcgcca
     2641 ttacgacggg tttatcaaga agggaaagat tcgcgggagg tcgtactgat atgttgctgg
     2701 taatgttggg tcccccgggt tcgggcaagg gcacgcaggc gtcccggctg gctgagagat
     2761 tggatttgca tcatctgtca accggcgata tgctcaggtc ggcggtcaaa aatgagaccg
     2821 aattaggcaa aaaagccaaa gaatatatgg ataaaggcga tctggttccg gacagcctga
     2881 tactggatat gatccgcgag acgataaaaa atgatcaagg cgcgggattt atcttcgacg
     2941 gatttccgcg cacgattccc caggccgagg ggctcgaccg gatgctccag tctgaaagtg
     3001 aagcgctgga tggggccttg aatctggaag tctcggatca ggaagttatc aagaggctct
     3061 ccgggcggta tttctgtccg cagtgccaga gaacctataa ctatccttcg aatctgccca
     3121 aaaaggaggg catctgcgat gaatgcggtg ttgagctgca gaagcgcaag g
//
LOCUS       DSEB01000381            3115 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003804, whole genome shotgun sequence.
ACCESSION   DSEB01000381 DSEB01000000
VERSION     DSEB01000381.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003804"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="ENO07_06855"
     CDS             complement(<1..1013)
                     /locus_tag="ENO07_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01723.1"
                     /translation="MNNFIKIIILFSISIIILFVPILSAEELIVYTANQGFFSRIYFL
                     NMDGSVHHYFEYENYHFCGLEVVNNQIYAAEAFAPRVLKVDPHTGDLDVIVDDWSLYY
                     FYGIAFDGTYFYLDEWDMNRYFLNGDKDGVASFDEDVFGLAWDGANLWMQNNANIIKC
                     YDVSTWPTLTELPDSAFTPPSPNCRGLWYDGTYFWTAESTDGVLGRIYKFDKEGNVID
                     QWMEPAYSGWGVCVFNFNELPDPPSNPYPEHGALDVDRDVILSWDCSDPEGQDLTYDI
                     HFGTDMIPPLAAADHPDTTFDPPDELDLETKYYWHITARDVYGDTTSGPVWNFTTVSG
                     PSY"
     gene            complement(1094..>3115)
                     /locus_tag="ENO07_06860"
     CDS             complement(1094..>3115)
                     /locus_tag="ENO07_06860"
                     /inference="COORDINATES: protein motif:HMM:PF05345.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01724.1"
                     /translation="PQSYEFIQVCVHSTSTYHTFRRPGDIWGETDVTEVKAIDPVAYF
                     YNLFACSNARYTSMNYMAGWYVFCQDYGLAAFGSAKTGSMLEFDDFYPSLGQQKPIGE
                     AYFDWWNAQSEYGYEDWEKCWYYGMTLIGDPTLRIQEKTTSRFLQYDEGSWDTFFPMP
                     GYNNNEYANTRFIAEDNCRLSEALLYLNWENPPPSRLYIWESDGIFPTSPIDSLDLYV
                     SQYNPYSWIIIDLSDMDLTFSAGEDFHIGINPINPGPGEEIDMFAAHGIDTIPVRSSV
                     RVNGEWKELDEISSYSKNFMIRATIVVEPLDEVEITTLTLPKADQDENYSQQIDAEGG
                     TPPYVWDMTAGALPAGLTLDAGSGIISGIPTGIDTAHFTVRATDNSVPQMSDIQHLTI
                     ITQTCIDSDGDGFGDPGYPDNTCITDNCPLIYNPDQNDQDVDGLGDSCDLCTDSDDDG
                     YGDPGFPANTCDDDNCTYVANAGQEDFDQDNVGDACDNCYDVPNSDQWDTDGDGVGDA
                     CDTEVVIHCDPLLHGQVGQPYYHEFLGLGGQRPYTWKKITGQFPYGVSFNTYDSVAVL
                     EGVPTWESSYSFTIELSDNSIPAGADTMICSIEIIPAEPEYICGDANADENVNVSDAV
                     YIINYVFIGGLEPEPYESGEVNCDGAVNVSDAVYIINYIFVGGTEPCDC"
BASE COUNT          844 a          793 c          695 g          783 t
ORIGIN      
        1 atatatgagg gccccgatac agtggtaaaa ttccagacag gtccggaggt ggtatcgcca
       61 tagacatcac gcgcggttat atgccagtaa tacttcgttt ccagatcgag ctcatcaggc
      121 gggtcgaaag tcgtatccgg atgatcggcc gccgctaatg gtggtatcat atcggtgccg
      181 aaatgtatgt cgtatgtcaa atcctgcccc tctggatcgg aacaatccca gctcagaata
      241 acatcacgat caacatccag cgcgccatgc tcggggtatg gattggaagg cggatcggga
      301 agctcattga agttgaaaac acacactccc catcctgaat acgccggctc catccactga
      361 tcgattacat ttccctcctt atcaaacttg tagatccttc ccagcacacc atcagtgctt
      421 tccgcagtcc agaaataagt gccgtcgtac catagccctc gacaattcgg agagggtggg
      481 gtgaaggcac tgtcaggcaa ttccgtcaag gttggccagg tggaaacatc atagcatttg
      541 atgatattgg cattattttg catccacaga tttgcgccgt cccaggccag cccgaaaaca
      601 tcctcatcga atgacgccac cccgtcttta tcgccgttaa ggaagtagcg gttcatatcc
      661 cattcatcga gataaaaata tgtgccgtca aatgctatcc cataaaagta gtacagtgac
      721 cagtcatcca ctatgacatc gagatcgcct gtatgcggat cgactttcag cacacgcggc
      781 gcgaatgcct cggcggcata gatttgatta ttcacgacct ccagaccgca gaaatgatag
      841 ttctcatatt cgaaatagtg atgcacgctg ccgtccatat tgagaaaata gatgcgcgag
      901 aagaagccct ggttggcggt atagactatt agttcttcag cggagagaat cggcacaaac
      961 aggatgataa tagaaatcga aaagagaatt atgattttga taaaattatt catatttttc
     1021 gcccttgagc agtatttgac aagcggtcat atatattata atagaattgg ctgtatagtc
     1081 aagccttcat attctaacaa tcacagggtt cagtccctcc aacgaaaata tagttgataa
     1141 tatatactgc atcgctgacg tttaccgcgc catcgcagtt gacctccccg gattcatagg
     1201 gctcgggctc caatccgccg ataaaaacat agttgataat gtaaaccgca tcggatacat
     1261 tcacattctc gtcggcattg gcatcgccgc agatatattc gggctcggca ggaataatct
     1321 caatagagca aatcatcgta tccgcgccgg ccggaatact gttatccgac agctcgatcg
     1381 tgaaactata tgacgattcc caggtaggca caccttcaag aacagccacg ctatcataag
     1441 tattaaaact cacaccataa ggaaattgac ccgtaatctt cttccatgta tatggcctct
     1501 gaccacccag cccaagaaat tcatggtagt aaggctgtcc aacctgacca tgcaggagag
     1561 gatcgcagtg aattacgacc tccgtatcgc aggcgtcccc aacaccatca ccatctgtat
     1621 cccattgatc ggagttcggc acatcataac aattgtcgca ggcatcaccg acattatcct
     1681 gatcaaaatc ctcctgcccc gcattggcaa cataagtgca gttatcatca tcgcatgtgt
     1741 tagcaggaaa cccgggatcg ccataaccgt catcgtcact gtcagtgcat aggtcgcagc
     1801 tgtcccccaa tccatcaaca tcctggtcgt tctggtctgg attatatatc agcgggcagt
     1861 tgtcggtgat acaggtattg tcgggataac caggatcgcc aaaaccatca ccatcgctgt
     1921 caatgcaggt ctgggttatt atagtaagat gctgtatatc gctcatctgc ggtacgctgt
     1981 tatcggtggc acgcactgtg aagtgagcgg tatctattcc ggtgggaatc cctgaaatga
     2041 tcccggagcc ggcatcaaga gtaagtcctg ccggcaacgc tccggccgtc atatcccaca
     2101 catacggtgg cgtcccacct tcggcatcaa tttgctgact ataattctcg tcctgatctg
     2161 cctttggaag tgtaagggtg gtgatctcaa cctcatcaag gggttcaaca actatggtgg
     2221 cccgaatcat gaagtttttg gagtaggatg atatctcatc cagttctttc cattctccat
     2281 ttaccctgac actgcttcta acaggaatag tgtcgatacc atgggccgca aacatatcta
     2341 tctcctcacc tggcccggga tttatcggat tgatccctat atgaaaatcc tctcccgccg
     2401 aaaatgtcag gtccatatcc gaaagatcaa taattatcca cgaatacgga ttgtattgtg
     2461 agacataaag atccaatgaa tcgattggag atgtggggaa aatgccatcg ctttcccaga
     2521 tatatagcct tgaaggaggc ggattttccc agttcaggta gagcaaggcc tctgaaagcc
     2581 ggcaattatc ttccgcaata aacctggtat ttgcatattc attgttgtta taccccggca
     2641 ttggaaagaa tgtgtcccag gatccctcat catattgcag aaatcgcgat gttgtttttt
     2701 cctgtattct caaggtcgga tcgccgatta gtgtcatccc gtaataccag cacttctccc
     2761 agtcttcata gccatattcc gactgcgcat tccaccagtc aaagtatgcc tcgccgatcg
     2821 gtttctgctg gccgagcgag ggataaaaat catcaaactc cagcatcgag ccggtcttgg
     2881 cgctgccaaa tgcagccagg ccataatcct gacagaaaac ataccatccg gccatataat
     2941 tcatggatgt atatctcgcg ttggaacagg cgaagaggtt ataaaaataa gcgaccggat
     3001 caatcgcttt gacttccgtg acatcggttt caccccagat atccccgggc ctgcggaaag
     3061 tatgatatgt tgaggtcgag tgtacgcaaa cctgaatgaa ctcataagac tgagg
//
LOCUS       DSEB01000382            3575 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002789, whole genome shotgun sequence.
ACCESSION   DSEB01000382 DSEB01000000
VERSION     DSEB01000382.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002789"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..475
                     /locus_tag="ENO07_06865"
     CDS             <1..475
                     /locus_tag="ENO07_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDS01725.1"
                     /translation="EISGRDLILIGGGLFLLAKSTMEIHENIEGHRKEKIERKVGASF
                     MMVILQIMMMDAIFSLDSVITAIGMVKQLGVMVAAIVIAIIVMMVFIGAVHRFVQKRP
                     TVKMLALSFLLLIGVALIGDGLHMHIPRGYIYFAIAFSTFVELLNQKIRYRTKAA"
     gene            complement(643..1500)
                     /locus_tag="ENO07_06870"
     CDS             complement(643..1500)
                     /locus_tag="ENO07_06870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HDS01726.1"
                     /translation="METLQTLGGGGYLMKRVSVMAVLAIFLMVPMLMAGNVVRLGEAT
                     MNDNIVTVPLMVSNDQELVAMDIPLEYSEGVTLTEVNFAERVDYFDMKLFNIDEANNR
                     VVIGLISMVYEQKPALAKAAISSDAVVANLVFRIDDMSLSGFQVTPFTSDAPSHELNL
                     IYNEYVDGVPYVREISPDFEGGNVSWVAQRAVPEQFSLAQNAPNPFNPSTDISYALPE
                     AGFVNVEIYNILGQRVKTLVNEHQDAGNYTVTWNGDDESGSTVASGVYFYRFTSGQYK
                     DIKKMVMMK"
     gene            complement(1663..1965)
                     /locus_tag="ENO07_06875"
     CDS             complement(1663..1965)
                     /locus_tag="ENO07_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDS01727.1"
                     /translation="MANKEKIEAALRECYDPEIPLNVLDLGLIYDIRLDNGDVEIDMT
                     LTAPGCPMHSLIARDIQSKVENVEGVENVKVNVVWDPPWTPERISEEGKKVLGFNK"
     gene            complement(2050..2988)
                     /locus_tag="ENO07_06880"
     CDS             complement(2050..2988)
                     /locus_tag="ENO07_06880"
                     /inference="COORDINATES: protein motif:HMM:PF13673.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDS01728.1"
                     /translation="MSEIKILQGPELSSLGLLLEKYRRGQNKFGDMIIGPFWVNLIVE
                     KITGGDEKALALTKDGQVEAFVIHKTGSNSVLASMVYVDKTERREALLSELVFRMRKI
                     YSAKSIVFGDPVPDMDIEAQKRIFLSAGFRVLERYKMEFSAPYEVKEVDRPPGFTFKK
                     YSKTLDRKVARLDQRAYRDHPDEPILEVLTDTGSNPPSLRIVRSGPAFFERSLSSFAL
                     SGSELAGGIYCTHMGKELWISNLVVDPHFRGQGLGKYLLFKVLKGMKPGGYRRCMLMV
                     SADNHSALNMYKKFRFKLKRNRFNFVYTTGQGVKNH"
     gene            3122..>3575
                     /locus_tag="ENO07_06885"
     CDS             3122..>3575
                     /locus_tag="ENO07_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HDS01729.1"
                     /translation="MADQDSTQQEFTPYVSSNEPQSEVTLRAIILGALISIIFGVGNA
                     YIGLRYGMTVSASLPAAVISMAILRGIFSRASVLENNMVQTIGSAGESLAAGVIFTFP
                     AFLIWEAHNTMGINEIFHLGGLEVVALSLLGGAMGIFLMIPLRRYLVEK"
BASE COUNT          904 a          900 c          797 g          974 t
ORIGIN      
        1 cgagatatcc ggtcgcgatt tgattctaat aggcggcggg ctgtttctac tggccaaaag
       61 caccatggaa attcatgaga atatcgaggg tcatagaaag gaaaagatcg agaggaaggt
      121 cggggcgtcc tttatgatgg taatccttca gataatgatg atggacgcta tcttctcgct
      181 cgactcggtc ataaccgcca tcggcatggt caagcaactg ggtgtcatgg tcgccgccat
      241 agtgattgcc ataattgtga tgatggtctt catcggcgct gttcatcgtt ttgtgcagaa
      301 acgtcccacg gtcaagatgc tcgcgctcag cttcctgctc ctaattggcg tggcgcttat
      361 cggcgatggc ctgcatatgc atatcccccg cggctatatt tatttcgcca tcgccttctc
      421 gactttcgtg gaattattaa atcaaaaaat ccgataccgc actaaagcgg cataaaatgc
      481 ttgtggaaac atgagtaggg caaaaccctt tagcggtttt gcctatttga ttcgtgacag
      541 gcagaatcac ttcgattagc tttgattctg ccctacgcca gaaaaaaagg cgccgatcta
      601 cggcgcctaa attcgaagaa aatccttctg acaagtgaac gattacttca tcatcaccat
      661 cttcttgatg tccttatact gtccggaagt aaagcgatag aagtagacac cactggcgac
      721 agtgctgccg gattcatcat caccattcca ggtaacagta taattacccg catcctgatg
      781 ctcattcaca agggtcttga ccctctggcc gagaatattg taaatctcga catttacaaa
      841 tccggcctcg ggcagagcgt atgagatatc ggttgacggg ttgaacgggt tcggtgcatt
      901 ctgggcaagg ctgaactgct ccggaactgc tctttgagca acccagctga catttccgcc
      961 ttcgaaatca ggactgatct cccttacgta cggcacgcca tccacatact cattgtaaat
     1021 gagattgagt tcgtgactgg gagcatccga ggtgaacgga gtaacctgga agccgctcag
     1081 agacatgtca tcaatacgga ataccaggtt ggcgactaca gcatcactgc tgatggcagc
     1141 cttggccaga gcaggcttct gctcataaac catgctgatc aggccaatga cgacgcgatt
     1201 gttggcttcg tcgatgttga acaacttcat atcgaaatag tcaactcttt cggcgaaatt
     1261 aacttcggtc agggtcacac cctcgctgta ttcgagcgga atatccatag ccacaagctc
     1321 ctgatcattg gataccatca agggcacagt gactatattg tcgttcatgg tagcctcgcc
     1381 gagacggaca acattgcctg ccatcagcat cggaaccatc agaaagattg ccagtacggc
     1441 cattacgcta acacgtttca tcaggtaacc accccctcct agggtttgta aagtttccat
     1501 aaaatactcc aaaacttttt attcttgaaa tcagttaatc attaatggcg tgcccagtta
     1561 cgtaagtact acatttctgt agctctgacc acagccaaga taaagtgcaa ttttcgattt
     1621 gtcaagaaat aaaagattct actcccatta cttttaagtt agctatttat taaaaccgag
     1681 aacttttttg ccttcctctg aaattcgctc cggggtccag ggcgggtccc agacaacatt
     1741 aaccttcaca ttctcaacgc cttccacatt ctcaaccttg ctctgaatat ccctggcgat
     1801 aagtgaatgc atggggcagc cgggggcggt aagggtcatg tcgatttcga catctccatt
     1861 gtccaaacgg atatcatata ttaaaccgag atcgagcacg tttaacggga tctcgggatc
     1921 gtaacactct ctcaatgcag cctcaatttt ctccttatta gccataataa aactcctgtt
     1981 cgttgttatt caccaatatt caacaacgaa cagcgtttct atgtttcctg tatgggggaa
     2041 aatgataacc taatgatttt taactccttg tcctgtagtg tatacaaaat tgaagcgatt
     2101 tcgtttcaat ttaaatctga atttcttata catattcaat gcggaatgat tatcagccga
     2161 gaccatcagc atgcatcgcc tgtaaccgcc cggtttcata cctttgagta ccttgaatag
     2221 caggtatttg cccaatccct gaccccgaaa atggggatcg acgaccaggt ttgaaatcca
     2281 cagttccttt cccatgtggg tgcagtatat gcccccggcc agctccgatc cggataatgc
     2341 aaaagagctc aggcttcgtt caaaaaacgc gggaccggag cggactatcc gcaatgaggg
     2401 cggattactg ccggtatctg tcagcacttc aaggatcggc tcatcgggat gatcccggta
     2461 tgcccgttga tccagccttg caactttccg gtcgagggtt ttgctgtatt tcttgaaagt
     2521 gaaacccggt ggacgatcga cttctttaac ttcgtaaggg gctgaaaact ccattttata
     2581 cctctccagg accctgaatc cggctgaaag aaatattcgc ttctgagctt cgatatccat
     2641 gtccggcaca ggatccccga agacgatcga ttttgcagaa tatatcttcc gcatccgaaa
     2701 gaccagttct gagagaagcg cctctcttct ttcggttttg tccacgtaaa ccatcgaggc
     2761 cagcacggag ttgcttccgg tcttatggat gacaaatgct tccacctgtc catcttttgt
     2821 caaggccaga gctttttcat cccctcccgt gatcttttcc acgatcaaat tcacccagaa
     2881 cggaccgatg atcatgtcgc cgaatttatt ctgccccctt ctgtatttct ccagaagcag
     2941 tcccaggctt gagagctcgg gcccctgtaa aatttttatc tcgctcattg atttccctcg
     3001 ataattacag catcaatcat tataagaaca aatgcagtgc tcgccaatca gtttttactt
     3061 gacacatcat cagcccgcaa atagttttga gaaaaattat tttagatctt gaaggaggat
     3121 catggccgat caagattcca ctcaacagga attcacaccc tatgtttcct cgaatgagcc
     3181 gcagtcagaa gtaaccctcc gagcaataat attaggcgcg ctgatttcga tcattttcgg
     3241 ggtcggcaac gcctatattg gtttaaggta cggcatgacc gtatcggcct cgctgccggc
     3301 ggccgttatt tccatggcga tcttgcgggg catattcagc cgggcttcgg ttctggaaaa
     3361 caatatggtg cagactatcg gttccgcggg tgagtccctg gcggcaggtg tgatatttac
     3421 ctttcccgca tttttgatct gggaggccca taacacaatg ggcatcaacg aaatttttca
     3481 tcttggcgga ttggaggtgg tagcgctttc ccttctcggc ggcgctatgg gtattttcct
     3541 gatgattccc ctccggcgct acctcgtgga aaaag
//
LOCUS       DSEB01000383            2835 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004669, whole genome shotgun sequence.
ACCESSION   DSEB01000383 DSEB01000000
VERSION     DSEB01000383.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004669"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..321
                     /locus_tag="ENO07_06890"
     CDS             <1..321
                     /locus_tag="ENO07_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDS01730.1"
                     /translation="LSKYIIMGSLCGLGKSGPNPFLSTVRYFRDEYLAHIIDKKCPGG
                     VCRDLIRFEINDKCTGCTACIAPCPVDAITGEKDKIHILDQEKCTKCGACYAVCQFDA
                     IDII"
     gene            336..1127
                     /locus_tag="ENO07_06895"
     CDS             336..1127
                     /locus_tag="ENO07_06895"
                     /inference="COORDINATES: protein motif:HMM:PF13510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HDS01731.1"
                     /translation="MKIQINGRWYEAREGEMVLDVLRREGIDVPALCAHEAVEPYGAC
                     RLCMVEITHEDWGGWKGNVTSCLYPVEDKLIVQTHTEDIIELRKTVLNLLLARCPNSP
                     EIQKLASEYGIDTTSFVPREDADNCIMCGLCTRVCTQMGFNAISVAGRGYGREIATPL
                     KEAPPDCVGCLACVKICPTHHIQYEDIGTTREIWGRQFELVKCKQCGKALITREFADY
                     LIENRDIPADYFDICDDCKRSGTARDFKKLVHWEEKEEAEEEVSA"
     gene            1124..1525
                     /locus_tag="ENO07_06900"
     CDS             1124..1525
                     /locus_tag="ENO07_06900"
                     /inference="COORDINATES: protein motif:HMM:PF13247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDS01732.1"
                     /translation="MTKRFLIRPALCIGCRTCELACSLTHQENKKPGRSRIYPIAVGR
                     EQWVPIACLQCDDPACVKSCLVDALRLNEELGYIELLQERCIKCRACVAACPFGCSLI
                     DEEQEIIVKCDLCEGNPACARFCPTKALEYK"
     gene            1670..2359
                     /locus_tag="ENO07_06905"
     CDS             1670..2359
                     /locus_tag="ENO07_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01733.1"
                     /translation="MRKIAIFFMIFVFSFVSGSAQESKIIEERYDSLTTKAIYTVITD
                     SSGQEIRHGIFIEWSEDGIRHLEGNYKQGKLDGVVTEYRPDLTRKSEITYENGEAVKE
                     VEYDSLGLYPQGVEDTLQQILQMQRVVIPTGREVEKMGYPRTSTIFINHGPATVWYYE
                     GGELMEKGNFMRAKPHGLMTRWSPFGRKEFEGTFVKGDRDGIWVYWDEEYNIDKIEIY
                     RNGSLVGGHKF"
     gene            2381..>2835
                     /locus_tag="ENO07_06910"
     CDS             2381..>2835
                     /locus_tag="ENO07_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01734.1"
                     /translation="MPENISDSVKTIAGEFDELAGDSAQAAQDTVSGFFESIRNIDPK
                     DWIVAISVVIGGFILGIILEIFIIRGLRRMAKSTKWEWDDVLIGSFKRAFVIWFTIIG
                     LYIALHVTISFNEVVENTVQKIIVALLILSVTIIVARAASGFVKLYSRST"
BASE COUNT          738 a          615 c          800 g          682 t
ORIGIN      
        1 ctatccaagt atataattat gggctcgctc tgcggattgg gtaaatccgg tccgaatccg
       61 ttcttgagca cggtcaggta tttccgcgac gaatacctgg cgcatataat cgacaagaaa
      121 tgtcccgggg gagtctgccg tgatctgatc aggttcgaga taaatgataa atgcaccggt
      181 tgtaccgcct gcatcgcgcc ctgcccggtg gatgcaatca ccggtgagaa ggacaagatt
      241 catattttgg atcaggaaaa atgcacgaaa tgcggcgcat gctatgctgt atgccagttt
      301 gatgcaatag atataatcta agagaggttg ataaggtgaa gatccagata aacggaagat
      361 ggtacgaggc gagagagggc gaaatggttc tggatgtcct gcgccgtgag ggaatcgatg
      421 ttcccgcact ttgcgctcac gaggcggtgg aaccgtacgg agcctgccgc ttatgtatgg
      481 ttgagatcac ccatgaagac tggggtggat ggaaaggcaa tgtcacctcc tgtttgtacc
      541 cggttgaaga taaattgatt gtccagacac atacagagga tataatcgaa ctgcgcaaga
      601 cggttctgaa tctgctcctg gcgcgctgcc cgaattctcc cgagatccag aagctggcat
      661 ccgaatatgg tatcgatacc accagcttcg taccccgcga ggatgccgac aactgcatca
      721 tgtgcggtct ctgtacccgt gtatgcacgc agatgggatt caatgcgatc tcggttgccg
      781 gaagaggcta tggccgtgaa atcgcaacac ccctgaagga ggctccgccc gattgtgtgg
      841 gatgcctggc ctgtgtcaag atctgtccca cccatcacat ccagtatgaa gatatcggaa
      901 ccacccgcga gatttgggga cggcagttcg aactggtcaa atgcaagcaa tgcgggaagg
      961 ccctgatcac cagagaattt gcggattacc tgatagaaaa tcgggatata ccggcagatt
     1021 atttcgatat ctgcgatgac tgcaaacgct ccggcacggc cagggatttc aagaaactgg
     1081 ttcactggga agaaaaggaa gaggctgagg aggaggtgtc ggcatgacca agcgatttct
     1141 gataagaccg gcgctctgca tcggatgccg cacctgcgaa ctggcctgtt cccttactca
     1201 ccaggagaat aagaagcccg ggcgcagcag gatttacccc atcgctgtcg gcagggagca
     1261 atgggtaccg attgcctgcc tgcaatgcga tgatccggcc tgtgtgaaat cgtgcctggt
     1321 ggacgcattg agattgaatg aggagctggg gtatatcgag cttttgcagg agagatgtat
     1381 caagtgccgg gcctgtgtgg cggcctgccc gtttggatgc tcgctgatcg atgaggagca
     1441 ggaaattatt gtcaaatgcg atctctgtga gggcaacccg gcctgtgcca gattctgccc
     1501 gaccaaagct cttgagtata aatgataata ggaatatggt ctcaactgcc ggtttctggc
     1561 cggcagtttt atttttggga taaattctgt tgtgtttagg ctataactta ttaacttatc
     1621 aattgagcga cagttttcac gatatccaat atcccgggag gtgatcaggt tgagaaaaat
     1681 agcaatattc tttatgatat ttgtgttttc gtttgtatcc ggcagtgccc aggagagtaa
     1741 aataatagaa gaacgatatg acagtctgac taccaaggcg atctacacgg tcataacgga
     1801 ttcttccggc caggaaatca ggcacggtat ctttattgaa tggtccgagg acggcatcag
     1861 gcatctggag ggtaactaca agcagggcaa gctcgacgga gtagtgactg aataccggcc
     1921 cgatttgacc cgcaagtctg agataactta tgagaatggc gaagcggtca aggaggttga
     1981 atacgattcg ctgggactgt atccgcaggg agtggaggat acccttcagc aaatacttca
     2041 gatgcagaga gtggtcattc ccaccggcag agaggtggaa aaaatgggtt atccgcgcac
     2101 cagcactatc ttcataaatc acggtcctgc gacagtctgg tactacgagg gcggagaatt
     2161 aatggagaag ggcaacttca tgcgcgcaaa gccgcacgga cttatgactc gctggagccc
     2221 ttttggaaga aaggaatttg agggtacatt tgtgaagggc gaccgggatg gaatctgggt
     2281 ttactgggac gaggaatata atatcgataa aatagaaata tacaggaacg gctctctggt
     2341 cgggggacac aagttttagg taaaatttgc aggtagcgat atgccggaga acataagcga
     2401 ctcagtaaaa acgatcgccg gggaatttga tgaactggca ggcgacagcg cccaggccgc
     2461 acaggacaca gtcagcggct tttttgaatc gatccggaac atcgatccta aggactggat
     2521 agttgcgata tcggtagtta tcgggggatt catccttgga ataattctgg agatttttat
     2581 aatccggggg ttgcgccgga tggcaaaaag caccaaatgg gaatgggatg atgttttaat
     2641 cgggtctttc aaacgcgcat ttgtgatctg gtttaccatt attggtctct acatcgctct
     2701 gcatgttacc atttctttta atgaggttgt cgaaaatacc gtccagaaaa ttatagtggc
     2761 tcttctgata ctctctgtca cgattatagt ggcccgggcc gcttcaggtt ttgttaagct
     2821 ttattcccgc agtac
//
LOCUS       DSEB01000384            2394 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1006805, whole genome shotgun sequence.
ACCESSION   DSEB01000384 DSEB01000000
VERSION     DSEB01000384.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1006805"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..492)
                     /locus_tag="ENO07_06915"
     CDS             complement(<1..492)
                     /locus_tag="ENO07_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01735.1"
                     /translation="MMDSSRKYLTIIFLLTALLFIYQPLEGQIVFGQPPSGEMKFIYQ
                     SWTIADSADNEISLNQWVLPLYGFVPVRDNVEIIFTSSTAGSNREFNDGSDASLTGLN
                     DTRLAVYGSFFEDRVVLGAGINLPTGKKSLDNDEIGVVNLLTESFLNFPIKNYGEGFG
                     ATLE"
     gene            complement(496..1797)
                     /locus_tag="ENO07_06920"
     CDS             complement(496..1797)
                     /locus_tag="ENO07_06920"
                     /inference="COORDINATES: protein motif:HMM:PF03783.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDS01736.1"
                     /translation="MKIRLLYLLTALSLILVSCSSGIYNRGKTELEAGNYQDAIAFFN
                     DAISENPDKADPWKFMGIAHYRAGNYGEAVDALKQAAILAPEDGSVNLFLGLSYERLG
                     ELEQAADIYRAYLDKHPDEEISGRIRHRVRYLTDKAVQQEVNQIISREKSIKTEEIPD
                     NTLAVLGFNPGNLTPRYSPLARGLSELLVIDLSKVPELKVVERLKLQAIMDEIQLTRS
                     EYFDKDRVPRVGKLIGASRIVSGQLSQQEDEVVIESGIIGVKDGFVNYPDDVEGDLQR
                     FFALQKNVARNILSTLGYELSPEEEEEFLAQPTNSFLAFLSYSLGLEYMDQNMYSLAQ
                     AQFDNALKEDPGFELAVKAREQVVGLSDYTGEVEPPGEIVEDFALYASAVTSAQTGQS
                     LRAIQTILGFQPDIGEDEGDNPYTLPVVGSGNVTINGSFDE"
     gene            complement(1805..>2394)
                     /locus_tag="ENO07_06925"
     CDS             complement(1805..>2394)
                     /locus_tag="ENO07_06925"
                     /inference="COORDINATES: protein motif:HMM:PF13036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01737.1"
                     /translation="SEDQEKLDPLCKGLMSMMIQDITKSEALRVVERDRINFLVEELE
                     LQQSDLTDKSTAVQMGKLVGAQTILMGGFMKVDKNDFKIFGRLVSVETGEVIKAEEVE
                     GDPDDVFELQKELVFKILDVMQIDVDKDTKEKINEGRDAKYEALYHYSLGLALEDKKE
                     YTEAYAEYKKALDLAPGYAEAMKKKERLEPLALKG"
BASE COUNT          585 a          661 c          534 g          614 t
ORIGIN      
        1 ctccagtgta gctccgaacc cctcgccgta attcttgatg ggaaaattta gaaatgattc
       61 tgtgagcagg ttcaccacac caatttcatc gttatccaga gatttcttgc cggtgggaag
      121 attgattccc gctcccagca ccactcgatc ttcgaagaat gagccataaa ccgccagccg
      181 ggtatcattc agccccgtaa gtgaagcgtc gctgccatca ttaaattccc ggttagagcc
      241 ggccgttgat gaggtgaaga tgatctccac attatcccgg acaggcacaa aaccgtaaag
      301 cggcagcacc cattgattca ggctaatttc attatcagca gaatctgcga tagtccagct
      361 ctggtatata aatttcattt ctcctgacgg cggctgtcca aatacgattt gcccctccaa
      421 aggctggtag ataaacagaa gagcggtcag aagaaagatg atcgtcaaat attttctact
      481 actatccatc ataacctact cgtcaaatga gccgttaata gtaacattgc cgctgcccac
      541 caccggcagg gtgtagggat tatccccttc atcctctccg atatcgggtt gaaaaccgag
      601 aatggtttga atagccctga ggctctgtcc agtctgcgcc gaggtcaccg cgctggcata
      661 cagcgcgaaa tcctccacaa tttcccccgg cggctcgact tcaccggtgt agtcggataa
      721 tccgacgacc tgttcgcgag ctttcacagc aagctcaaaa ccggggtctt ctttgagagc
      781 attgtcgaac tgggcctgag ccagcgaata catattctga tccatatatt ccagccccag
      841 gctgtacgac aggaatgcca gaaaagagtt tgtgggctgc gccaggaact cctcctcttc
      901 ttcgggagaa agctcgtaac caagcgtgct taaaatattt ctggccacat ttttctgtag
      961 cgcgaagaac ctctgcaggt ctccctcgac atcatcggga taatttacaa aaccatcttt
     1021 aacgccgata atgccggatt caattactac ctcgtcctct tgctgagaga gctgtccgga
     1081 gacaatccgg gacgctccga tcagctttcc cacgcgggga acccggtcct tgtcgaagta
     1141 ttccgatctg gtgagctgga tctcgtccat gatggcctga agcttcagcc tttccacgac
     1201 tttaagctcc ggaaccttgc tcaggtctat aaccagcaac tcggaaaggc cccgagccag
     1261 gggggaatag cgaggcgtaa ggttgcctgg attgaaaccc aggacagcca gagtattatc
     1321 cggtatctcc tcggtcttga tcgatttctc ccgggagatt atctggttca cttcctgctg
     1381 gaccgccttg tcggtcaggt acctgacacg atgcctgatc cggccggaga tctcctcatc
     1441 cggatgctta tcgaggtaag ctcgatagat atccgcggcc tgctccagtt ctcccaaccg
     1501 ctcatatgac agacccagaa ataaattcac tgagccgtct tcaggtgcca gaattgccgc
     1561 ctgtttgaga gcatcaacag cctctccata atttccggcc cgataatgag caatacccat
     1621 gaatttccag gggtctgctt tgtcgggatt ctcggaaatt gcatcattaa aaaaggcaat
     1681 tgcatcctga tagtttcctg cctcaagttc cgtcttgccg cggttatata ttccgcttga
     1741 gcaggaaacc aaaataagtg ataaagctgt caggagatac agtaacctaa ttttcaatag
     1801 gcatttatcc tttcaaggct aatggttcca gacgttcctt tttcttcatg gcctcagcat
     1861 atccgggcgc cagatcgagc gccttcttat attctgcata ggcctcagta tattctttct
     1921 tatcttccag agccagcccc agggaataat ggtaaagggc ctcatatttg gcatcccggc
     1981 cctcgttaat cttctcctta gtatccttgt cgacatctat ctgcataaca tcaaggattt
     2041 taaagactaa ttccttctgt aactcaaaga cgtcgtcggg gtctccctcg acctcctcag
     2101 ccttgatgac ctcgcccgtt tccaccgaga caagacggcc aaatattttg aagtcatttt
     2161 tatcgacctt cataaagccg cccataagga tagtctgggc gccgaccagc ttacccatct
     2221 gaaccgcggt gctcttatcg gtcaggtcgg actgctgcag ttcgagctcc tccacaagaa
     2281 aattgatacg gtcacgttcc accacacgga gcgcttcaga cttggtgata tcctggatca
     2341 tcatggacat caaacccttg cacaacggat caagtttttc ctggtcttca gaga
//
LOCUS       DSEB01000385            9908 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000299, whole genome shotgun sequence.
ACCESSION   DSEB01000385 DSEB01000000
VERSION     DSEB01000385.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9908)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9908)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9908
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000299"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1452
                     /locus_tag="ENO07_06930"
     CDS             <1..1452
                     /locus_tag="ENO07_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase"
                     /protein_id="HDS01738.1"
                     /translation="EEYKQSLRKMRPNIYKFGRLIEDVTTDPVTKWTVEGHSQIYKAQ
                     NDPELKDKVTVISHLTGEPISRYLSIIRSAQEQIDNSKMKRLMFQLSGTCTGGRCAGW
                     TAINAMWATTYDMDNDLKTNYHQRLTEWLKSAQERDITISGALTDPKGDRTKKPSQQD
                     DPDMNLRIVEKRDDGIVVRGAKVMICGIAAANEVFVLPGSSYKEEDADWAVSFVVPKD
                     IEGLTIVEARHPNDTREQEEQDSFDNPVKQGGITQAYLFFEDVFIPNERVFMAGEYKY
                     TGSAVVNFILPYRAAIGGCVAGQGDIKVGAAILTARANGLPSKVFSDKLTQMYINNET
                     TFGMGIAAAVLGKKHPSGAWQPDPLIANINKALVARLPYETSVLAQDIAGGIGETGCM
                     PSYVDYNDENYGHLIKKYLKAAASAETRMRAARLVEWTTTGAGIPGCMHGGGSADGAK
                     MFVRLFSGMENSVKVAKRLAGIEEELPEPAKKK"
     gene            1562..2464
                     /locus_tag="ENO07_06935"
     CDS             1562..2464
                     /locus_tag="ENO07_06935"
                     /inference="COORDINATES: protein motif:HMM:PF11276.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HDS01739.1"
                     /translation="MIISSMEALMKKGLILLAALLFTSSGLYAEPWEKTLTFDLKLTQ
                     TAYSDSWTGGEAGNISWVSTLNGIFVKQITETINNRTTLKLAFGQTHIQDAETKEWDK
                     PEKSTDQIDIENLSRLTFGGYVDPYFAVRLETQFLDASVDSIKRYFNPLLLTESVGAS
                     RMFYKRDKDEILSRLGFAVKQTINNIIVDTAASEKDWNTRTDAGFESVTDVIYSFSES
                     LGYIGKLTLYKALFFSEKDEVEGTPEEDYWKAVDVNWENTISASVIKYVTVSLYVQFL
                     YDKQIEKKGRFKQTLGLGLTYKMF"
     gene            2528..3229
                     /locus_tag="ENO07_06940"
     CDS             2528..3229
                     /locus_tag="ENO07_06940"
                     /inference="COORDINATES: protein motif:HMM:PF02308.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDS01740.1"
                     /translation="MNDLALVLDVDVVIKMALAVLLGALIGVEREYHGRPAGLRTHIL
                     VCLGSTLLIIGSRSLPIAIEEDPGFIGNFVIDPARLAAGIITGIGFLGAGVVLKTKDF
                     IRGVTTAACIWFAAAIGIVIGLGFYLIAVISTILALFVLLFLVKISSTIYPHTYKHII
                     IASRDTEMEKFAENCENILESYQVTILDTDYFRDKQGGEFTLDYYIRSRSQEIGLTLM
                     QKFSGLPEVTQIRIN"
     gene            3270..3329
                     /locus_tag="ENO07_06945"
                     /pseudo
     CDS             3270..3329
                     /locus_tag="ENO07_06945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01376.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing start; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3636..4439
                     /locus_tag="ENO07_06950"
     CDS             3636..4439
                     /locus_tag="ENO07_06950"
                     /inference="COORDINATES: protein motif:HMM:PF01261.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDS01741.1"
                     /translation="MRIGISTSFNYQIPLAENLEAIAAAGFKFISVGGNIRHSNYNTA
                     EGRGKLLEYTDRLGLDIDSVHAPYDPTCDLTQTQDIFAQNAVIEIKRSIAAASELGVE
                     KLIVHLSTFRPRKLSERMARIKLTVAEILEFAEERRVLIALENLDSDSEVLFKYAMDL
                     FESDYLRFCYDNGHEMLYNESMDLLKKYYQRMIVMHFHDNDGRRDLHLIPFEGKLNLP
                     ALAGQLNKLERIPDITLECEMENCNYATAEKFLEAAYNKGMQFTEMLKR"
     gene            4570..5736
                     /locus_tag="ENO07_06955"
     CDS             4570..5736
                     /locus_tag="ENO07_06955"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00534.18,HMM:PF13439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDS01742.1"
                     /translation="MMSKIKILYIIDEITDVTAGTERQLSLLLRNLDQDIFEPHLMTL
                     RATEWLDSECYPCPIHNLEMGPVLSLSGFNKLTELRYFIADNNFQIVQTYFPDANVIG
                     ALAARQAGCKIIISTRRNTGFFYNSSLLMATKFANRHVSRFLANSELVVKAISEKEGI
                     DPERFSVIYNGLEVDRFRFSEQDVRSARVFMGTAEHENLVGVVANLRPVKALEVFLRA
                     AARISHTRDDIRFVIIGGGDETYAAELKRMARELSLGDNIKFLGAVGNPSSFIKNFDV
                     GVLTSRAEGLSNTLMEYGALGVASVATDVGGNSEIIRHGETGLLVPPDDPEKVADAID
                     QLLADQNKRERMGSNAARAAWSKFNLNVTVKLHQALYTKMYDQVVTDRELAEAE"
     gene            5820..7208
                     /locus_tag="ENO07_06960"
     CDS             5820..7208
                     /locus_tag="ENO07_06960"
                     /inference="COORDINATES: protein motif:HMM:PF04932.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01743.1"
                     /translation="MEHLQETAFSDVRPITSRNIVYIFIVLLSIVFGILNFIVPSHYL
                     AAILIMAVAAVILFLHPFAGLLVYQFIIIIQPGVIFPSLGLLHLERLMAIFLIISLII
                     NIRLRRGRLIITDHKLLLFMMFFILSMLITIPVSYWPAQSLENVIEFLKNFAFILLII
                     NILKTGRRLRIFVWEYLLLIGYMAASSLLAYKTGNVVEAQGIIRAHGLAGTDPNTLSA
                     TLGLSLPFHFMLFRLEKKKYLKALSVFIVIITVYTIILTGSRSGFLGLGTAVILSWWF
                     LPGRFIRLFLLLTAGFAVIVLMPSQYQERYASIFSRERDASSQERIEVWKKGIRMFYD
                     HPFLGVGTGAFGTANAEDYSEDRKSYLKAHNLYIQAAAELGIVGLAAFFSYVGAMLYY
                     IRKYRKYIVDKFRKKTWSWGLLTAMQISTLALLVVGMFGHSLYRSNWFFYGALTMAMG
                     LLLRNNRLEEEV"
     gene            7213..8586
                     /locus_tag="ENO07_06965"
     CDS             7213..8586
                     /locus_tag="ENO07_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HDS01744.1"
                     /translation="MSLNHYFTNPIVMLAYYFWNRISKTDRLGILRALDKSQYYDIER
                     LRSIQLKKMRNVIKNAFHSSPFWQEKFKDIGLEEGDIEGLVSTDKIPKLTREDVINNK
                     DRIVSGAFSKDELTENVSGGTTGSSLSYYLSRHRIDYRSASTLRHNLWTSYRIGDRLA
                     TVWGAPADMESHRSLRTKMRDIFWGASINFDSNIVTDDAVRKFAREWDRFRPEILLAY
                     SGAVDFFVNHARDMKLSFAPPRAIITSAEVLTPGARKNIEDFFEAEVFNRYGCREFSV
                     IASECRYHNGLHINAETLLLEFEPVLEALDNRRHCRVLVTDLENLAFPFIRYELGDVV
                     VLSDEEDRCPCGRNLPRIEEVGGRIMDYLKLPGGRYLSCTALTATCFPRARGIIQSRI
                     VQKKIDKIIVQIVKNNDFDEESYNILKQDLAKVLGEGIELVFEFPEQIKRERSGKYRF
                     VISEVAD"
     gene            8588..>9908
                     /locus_tag="ENO07_06970"
     CDS             8588..>9908
                     /locus_tag="ENO07_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HDS01745.1"
                     /translation="MPFLSWLIRRTTLPMYLRHRGEAGQLSYLDEFARIEKLSLDEIN
                     QIQLHAFHKLMQHAYRFCRYYREIFRDIGAAPWDLNSIGDLQKFPVLTKELIRENLDG
                     ILAKNIDPRRRLRSATGGSTGRPLVFYRDQTCREKKLAMQLNFMRWYGFLPGDKHLFF
                     WGAARDYDRLDTFKARIVQRAATRRWFVNMNDLKTENFDRTVERLANLRPRLVSAYPN
                     IIYSFAQEVEKRNKRVKFDKIVVTAEQLFEHQREKIEKVFGAEVFEQYGSREFGTIAG
                     ECLYHDGLHYFAPGVVLETVTEEGRPSGENPGNLLVTDLWNYAMPLIRYQVGDLVRLE
                     HAPCKCGCELPRVGAVAGRVVDAVIKPGGEAIAGQALIAVIRKSEIDAQTQIIQKGPV
                     EFVLNYVSGRKLPEDKLSFIRSGFNSVMENQVKIDFNRVDKIERDRSG"
BASE COUNT         2610 a         2175 c         2591 g         2532 t
ORIGIN      
        1 gaggaatata aacaaagcct gcggaagatg cggccgaata tttacaaatt cggcaggcta
       61 attgaggacg ttacaaccga tccggtcacg aaatggacgg tggaaggaca ttcgcagatt
      121 tataaagcgc agaatgatcc ggaactgaaa gataaggtga ccgtaatatc acacctcaca
      181 ggtgagccga tttcgagata cctgtcgatc atccgttcag cgcaggagca gattgataat
      241 tcgaagatga aacgtctgat gtttcaactc tcgggaacct gtaccggcgg gagatgtgcg
      301 ggatggaccg ccataaatgc gatgtgggcg actacatatg atatggataa tgatcttaag
      361 accaattatc atcagcgcct gaccgaatgg ctcaagtccg cccaggaaag ggatattaca
      421 atttccgggg cccttaccga tcctaaaggc gaccgtacaa aaaaaccctc acagcaggat
      481 gatccggata tgaacctccg cattgtcgag aagcgtgatg acggtattgt tgtgcggggc
      541 gccaaggtaa tgatttgcgg gatcgcggcc gccaacgagg tctttgttct gcccggcagc
      601 agctacaagg aggaggatgc cgattgggca gtgtcatttg tagttcccaa ggatatagag
      661 ggtctcacaa tcgttgaagc gcgtcatccc aatgacaccc gcgaacagga agagcaggat
      721 tcgtttgaca atccggtcaa acagggcgga ataacacagg cttacctgtt ctttgaggat
      781 gtttttattc ccaatgagag ggttttcatg gccggcgaat acaaatatac tggttccgcc
      841 gtggttaact tcatcctgcc gtatcgcgcc gcaattggcg gatgtgtggc cggacagggc
      901 gatattaagg tcggtgcggc gatattgacc gcccgcgcta acggattgcc gtccaaagta
      961 ttttccgata aacttacgca gatgtatatc aataatgaga ctaccttcgg aatggggatc
     1021 gccgctgccg tgctcgggaa aaagcacccc tcgggcgcat ggcagccgga tccattgatt
     1081 gcgaatataa ataaggcgct tgtggccaga ctgccctatg aaacctcggt tctggcgcag
     1141 gatattgcgg gaggtatcgg agaaaccgga tgtatgcctt cctatgttga ttataacgac
     1201 gaaaactacg ggcacctgat aaagaagtat ctgaaggctg cggcttcggc cgagacacgt
     1261 atgcgcgccg cccggctggt ggaatggacc accaccggtg caggtatccc cggatgtatg
     1321 catgggggag gttctgctga cggcgctaag atgtttgtga ggctgttctc cgggatggaa
     1381 aattccgtca aggtggctaa acgtctggca ggaattgaag aggaactgcc ggaaccggcg
     1441 aagaagaaat aagaatatta ttataagcgg agcgtttttc ggcgctccgc tttctcttca
     1501 aaaaaataat tgttttaata gcttatcaca ctggcgattt atacattccc cgtaaagaag
     1561 aatgatcata tcaagcatgg aggcacttat gaaaaaagga ctgattcttc ttgccgccct
     1621 tctatttact tcgtccggct tgtatgccga accgtgggag aaaaccctga ccttcgatct
     1681 gaaattaacc cagactgcct actccgacag ctggacgggt ggtgaggcgg gcaatataag
     1741 ctgggtttcg acccttaacg gaatcttcgt gaagcagatc acggagacca ttaacaacag
     1801 gaccaccctc aaacttgcct tcggtcagac tcatatccag gatgctgaaa ccaaggaatg
     1861 ggataagcca gaaaaatcga ccgatcagat cgatatcgag aatctctccc gcctgacttt
     1921 tggcggttat gttgaccctt atttcgcggt caggctcgag acccagtttc tcgacgcctc
     1981 agttgattca atcaaaaggt atttcaatcc tctgctcctg acagaatcgg tgggcgcctc
     2041 aagaatgttc tataagcggg acaaagacga aatcctgagc cgtctcggct ttgcagtgaa
     2101 gcagacaata aacaacatta tcgtggatac tgccgcaagc gagaaagatt ggaatacccg
     2161 cactgatgcc ggttttgagt cagtcaccga tgtaatctat agtttctcgg aaagcctggg
     2221 ctacattggc aagctcacgc tttacaaagc gcttttcttc tcggaaaagg acgaagttga
     2281 gggaaccccg gaagaagatt actggaaagc tgttgatgtt aactgggaaa atacgatctc
     2341 ggcctcggtg atcaaatatg tgaccgtttc gctgtatgtc cagtttttgt atgataaaca
     2401 aattgagaag aagggccgtt tcaaacagac tctcggtctg ggcctcactt acaagatgtt
     2461 ctaacgggta gtttggagtg ttctgctgga agcctcgcag gatattctaa atttggggga
     2521 aagcaagatg aatgatctcg ctcttgtctt agatgttgat gtggttatca agatggccct
     2581 ggcggttttg ttgggggcgc tgatcggggt ggagcgcgaa tatcatggcc ggcccgcagg
     2641 gctcaggacc catattctcg tctgtctggg gtcaacgctc ttgattatcg gatccaggtc
     2701 cctgcccatc gcaatagagg aggatcccgg ttttatcggt aatttcgtaa tcgatccagc
     2761 ccgcctggcg gccggaatta tcaccggaat tggtttcctg ggcgccggtg tggtccttaa
     2821 aacgaaagac ttcattcgcg gtgttaccac cgccgcatgt atatggtttg ctgccgcaat
     2881 tggaatagta atcggtctgg gattctattt gatcgcggtc atcagtacca tccttgccct
     2941 attcgtactt ctgtttctgg taaagatcag ctccacaatc tatccccata catacaagca
     3001 tattattatt gcctcccggg acacagagat ggagaagttt gccgaaaact gtgaaaatat
     3061 tctggaaagc tatcaggtca cgatactcga cactgattat ttcagggata agcagggtgg
     3121 ggagtttacg ctggattatt atatccgcag ccgcagccag gagatcgggc tgacgttgat
     3181 gcagaaattt tccggcttgc cggaggtgac ccagatcagg atcaactaaa aaaaacaccg
     3241 gcgagatttc tctcgccggc tagtgggcaa gcaactcagg ctgctgcctg aggggaggtg
     3301 tgatttattc tacatctcgt agaaattagc ctcagttaag cccttcttcg gcaccggata
     3361 cgttctatcg attcaattac aaatatctat cataatctaa acaggcaaaa tacatacccg
     3421 ccccggatat tattgcctaa tatgatggca gtcaatatgg tagcagattg ggagagccct
     3481 gcgcaggata aaaaaatctc aaatatatcg acttaaccca ataaatgctg atcaaaatcc
     3541 aataaaaagt tcaaaatcaa tacacctgag gggaacagta tgacccccca atcaaaaaac
     3601 ttgacaaaag atatattgtt tattttctaa tctccatgag aatcggaatt tccaccagct
     3661 tcaactatca gattcctctg gcagaaaatc tcgaggccat tgcggctgcg ggcttcaaat
     3721 ttatatcggt gggcgggaat atcaggcatt cgaattataa taccgctgag ggccgcggta
     3781 aactcctgga atataccgat agactggggc tcgacataga ctccgttcat gcgccatatg
     3841 atccgacctg cgatcttacc cagacccagg atatatttgc ccagaacgct gtcatagaaa
     3901 tcaaaagaag tatcgcggcc gcatccgaac tgggggtcga aaaactgata gtccacctct
     3961 caacattcag gccccgaaag ctgtctgaga gaatggccag gataaagctc actgttgctg
     4021 aaattctcga atttgccgag gaaaggaggg ttttaatagc gctggagaat ctcgattctg
     4081 actcggaggt cttatttaaa tatgcaatgg atctgtttga atcagattat ttaagatttt
     4141 gctatgacaa tgggcatgaa atgctttata atgagtccat ggatttgttg aagaaatatt
     4201 accagcgcat gattgtgatg cattttcatg acaacgacgg ccgcagagat ttacacctga
     4261 ttccatttga aggtaaactg aatcttccgg cgctggcagg gcaactcaat aagctggaaa
     4321 ggattccgga tattaccctg gaatgcgaga tggaaaattg caattatgcg acggcagaga
     4381 aattcctgga ggccgcatat aataagggta tgcagtttac ggaaatgctc aaacgctgac
     4441 atctgaacct attcaagata atgagttaag gtaaataatt gtaagattgg acttgctgcc
     4501 tgccgatttt ataataaaag agcaggttag ttcctttgtg ccgaatcggt ttcggcgatt
     4561 gtaaagagca tgatgagcaa aatcaaaatt ctatacatta tcgatgagat aacagacgta
     4621 actgccggaa ctgaaagaca gttatctctg cttctgagga atctggatca ggatattttt
     4681 gaaccccatc ttatgaccct cagggccacg gagtggctgg acagcgaatg ttatccctgc
     4741 cccatccata atcttgagat gggcccggta ttgtccctgt cgggttttaa taaactgacg
     4801 gagcttagat actttatcgc cgataataat tttcagatcg tgcagacgta tttccctgat
     4861 gccaatgtga tcggtgcgct ggctgcacgg caggcgggat gtaagattat aatttcaact
     4921 cgcaggaata ccggattctt ctacaattca agcctgctca tggccaccaa attcgccaat
     4981 cgtcatgttt ccaggttcct tgccaattcc gaacttgtgg tcaaggccat ttcggagaaa
     5041 gagggaatcg atccggagcg tttttcagta atctataacg gtctcgaggt tgaccgtttc
     5101 aggttttccg agcaggatgt aaggagtgcg cgggttttta tggggacggc ggagcatgag
     5161 aatctggtcg gagtagtggc caatttgaga ccggtgaaag cgcttgaagt ttttctgagg
     5221 gcggccgcca gaatcagtca tacacgtgac gacatcaggt ttgtgataat aggcgggggg
     5281 gatgaaacct atgcggccga actgaagcgg atggcgcggg aattgtctct gggtgacaat
     5341 ataaaatttc ttggcgcggt cgggaatccg agctcattta taaagaattt cgatgtggga
     5401 gtcctgactt cacgggcaga agggctctcg aacaccctga tggaatatgg cgcgctgggt
     5461 gtcgcctctg tggctacaga tgtgggtggg aactcggaga taattcggca tggagagacc
     5521 ggcctgctgg taccgccgga tgatccggaa aaagttgctg atgcaataga tcaactgctc
     5581 gcggatcaaa ataaacgcga gaggatggga tcaaatgccg ccagggcggc ctggtccaaa
     5641 tttaatctaa atgtgacggt gaaacttcac caggccctgt acacgaagat gtatgaccag
     5701 gtggtcactg acagggagct ggccgaggct gaatgagctc tcgatcgttc tcggcaggcg
     5761 ttgcatttta ccatatttca aatactaaaa agtaaattct cgtaagcaag tccgctgtta
     5821 tggagcattt acaggagaca gctttttcgg acgttcggcc gataacatcg agaaacatag
     5881 tctatatctt catagtcctg ctttcaattg ttttcggcat tctcaatttt attgttccct
     5941 cccattatct tgcggcgata ctaataatgg ctgtagcggc tgttattctg ttcctgcacc
     6001 cattcgcggg cctgctggtc tatcagttca ttattatcat ccagcccggg gtgatttttc
     6061 catcgctggg tttgcttcat ctcgaaaggc tcatggccat attcctgatc atctccctga
     6121 ttataaatat caggctcagg aggggcaggt tgataatcac agatcataaa ctccttctct
     6181 tcatgatgtt tttcatcttg tccatgctga ttactattcc ggtatcctac tggcccgcgc
     6241 aatcgctgga aaatgtgatt gaatttttga agaacttcgc tttcatactg ctcataataa
     6301 atattctcaa gacaggcagg cgactgcgga tatttgtgtg ggaatatctg ctgctgatcg
     6361 gctatatggc cgcaagctca ttgctcgcat ataagaccgg caatgtggtt gaggcccagg
     6421 gaataatccg cgctcatggc ttggcgggaa cagatcccaa tacactttct gcaaccctgg
     6481 gattgtcgct gccattccac ttcatgctct tcagactgga gaagaaaaaa tatctcaaag
     6541 cgctctctgt gttcatagtc ataattacag tctatacaat tatcctgaca ggatcgaggt
     6601 ccggtttcct ggggctcgga actgctgtca tattgagctg gtggttcttg cccggacgtt
     6661 tcatcaggct gtttcttctc cttactgcag gatttgcagt aatagtcctt atgccgtcgc
     6721 agtaccagga gcgctatgcc tcgatatttt cgagagaaag ggatgcctcg tcgcaggagc
     6781 ggatcgaggt ctggaagaaa ggcatacgta tgttctatga ccatcccttt ttgggcgtgg
     6841 gcaccggcgc ttttggaact gccaatgccg aggattattc agaggatcgc aagagctatc
     6901 ttaaggcgca taatttgtac atacaggcag ccgccgaatt gggaattgtt gggctggcgg
     6961 catttttttc ctatgtgggt gcaatgcttt actacattcg aaaatataga aaatatatag
     7021 tcgataagtt caggaagaag acctggtcct ggggtctttt gactgcgatg cagatttcga
     7081 ccctggcctt actggtggta gggatgttcg ggcatagcct gtaccgatcg aactggttct
     7141 tctacggcgc cttgacgatg gcgatgggat tgcttttgcg caacaatcgg ctcgaggagg
     7201 aggtataggc agatgtcttt gaaccattat ttcactaacc cgattgtaat gcttgcttat
     7261 tatttctgga acagaataag caagactgat cgtctgggca ttcttcgcgc cctggataaa
     7321 agccagtatt atgatataga acggctgcgt tcaatacagc tcaaaaagat gcgcaatgtt
     7381 atcaaaaacg catttcacag ctcaccgttc tggcaagaga agttcaaaga tatcggcctg
     7441 gaagaggggg atatagaggg tcttgttagc actgataaga taccgaagct gacccgtgag
     7501 gatgtcatca ataataaaga tagaattgtg tcgggggcat tttccaaaga tgaattgact
     7561 gaaaatgtct cgggcggaac aaccggtagc tccttatcat attacctgag caggcataga
     7621 attgattaca gaagcgcgag tacgttaaga cataacctgt ggacctcata tcgtatcgga
     7681 gacagattgg cgactgtctg gggagcgcct gccgatatgg aatctcacag aagtctcagg
     7741 acaaagatgc gcgatatatt ctggggcgct tcaattaatt ttgacagcaa catagtgacc
     7801 gatgatgcgg tcaggaaatt cgcccgtgag tgggacagat tcaggcccga gattcttctg
     7861 gcctattccg gcgcggtcga tttctttgta aatcatgcca gagatatgaa actctctttc
     7921 gctccgccgc gcgccattat tacatctgca gaggtcctca cacccggggc ccgcaaaaat
     7981 atcgaggatt tcttcgaggc cgaagttttt aatcgctatg gctgccgtga gttttcagtt
     8041 attgcctcgg agtgccggta tcataatggc cttcacatta atgccgagac attactgctg
     8101 gagttcgaac ctgtccttga agcactggac aaccgcaggc attgcagggt ccttgtcact
     8161 gatctggaga acctggcttt tccttttatc aggtacgagc tcggagacgt ggttgtgctt
     8221 tctgacgagg aagaccgctg cccatgcgga agaaacctgc caaggatcga ggaggttggc
     8281 ggaaggatta tggattattt gaagctcccc ggcggacgtt atctctcctg tactgcgctg
     8341 actgcgacat gtttccccag ggccagagga ataatccaat ccaggattgt acagaaaaaa
     8401 attgacaaaa tcattgtgca gattgtcaaa aacaacgact ttgatgagga atcttataat
     8461 atattaaaac aagacctggc aaaagttttg ggcgagggaa tcgaacttgt gttcgaattt
     8521 cccgagcaaa taaagagaga acgatcgggg aaataccgtt tcgtcataag cgaggttgcg
     8581 gattaagatg ccatttctat cctggctgat cagaaggacc accttgccga tgtatctcag
     8641 gcacaggggg gaagcggggc agctttcgta tctggatgag ttcgcccgga tcgaaaaact
     8701 ctcccttgac gagatcaatc agattcaact gcacgctttt cataaattga tgcagcatgc
     8761 ttaccggttt tgtcgatatt acagggaaat attccgggat atcggagcgg ccccctggga
     8821 tctgaattcg attggagatc tgcagaagtt ccctgtgctg acgaaagaac tgatcaggga
     8881 aaatttggat ggtatcctgg cgaaaaacat cgatccccgg agaagactgc gttccgccac
     8941 aggcggctca accggcaggc ccctggtttt ttatcgggat caaacctgca gggagaagaa
     9001 acttgccatg cagttgaatt ttatgcgctg gtacggattc ctgcccgggg acaagcatct
     9061 cttcttctgg ggagcagccc gggattacga taggcttgat acattcaagg ccaggattgt
     9121 gcagagggcg gccacacgca ggtggtttgt aaacatgaat gatcttaaaa cggagaactt
     9181 cgacaggact gtcgagcggc ttgctaactt gagaccccga ttagtttccg cttatccgaa
     9241 tatcatttat tcttttgcac aagaggtgga gaagagaaat aagagagtta aattcgataa
     9301 aattgttgtg accgcggagc agctatttga gcatcagcgt gagaagatcg agaaggtctt
     9361 cggggcggag gttttcgagc aatatggcag tcgtgaattc gggacgatag ccggcgaatg
     9421 cctttatcac gacggcctgc actattttgc tcccggtgtt gttttggaaa ctgtgacgga
     9481 ggagggcaga ccctccggtg aaaatcccgg caatcttctc gtaactgact tatggaatta
     9541 tgcgatgccg ctgattcgtt atcaggtggg ggatctggtg aggctggaac atgcgccctg
     9601 caagtgcggc tgcgaactgc ccagggtagg cgctgtcgcg gggcgggttg tagatgcggt
     9661 aatcaagcct gggggtgagg caattgccgg gcaggccctc attgcagtga tacgaaagtc
     9721 tgaaatcgat gcccagacac agataataca gaaagggccc gtcgaatttg tgctcaatta
     9781 tgtttccggg aggaagctac cggaggataa gctaagtttt attcgcagcg gttttaattc
     9841 ggtaatggaa aaccaggtaa aaatagattt taatagagtg gataagattg agagggatag
     9901 atcaggaa
//
LOCUS       DSEB01000386            2294 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1007470, whole genome shotgun sequence.
ACCESSION   DSEB01000386 DSEB01000000
VERSION     DSEB01000386.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1007470"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..180)
                     /locus_tag="ENO07_06975"
     CDS             complement(<1..180)
                     /locus_tag="ENO07_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01746.1"
                     /translation="MEITYRNLRPEDYDSLIQVWKASGLPVRLTGRDSREKITEHMKE
                     QPDFFTGAFDRGRLIG"
     gene            complement(181..1161)
                     /gene="ilvC"
                     /locus_tag="ENO07_06980"
     CDS             complement(181..1161)
                     /gene="ilvC"
                     /locus_tag="ENO07_06980"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:PRK05479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDS01747.1"
                     /translation="MQKIYRDQNADIKYIRNKQILIIGYGSQGRAFALNLRDSAIEVN
                     ICIKLESLSANAARAEGLKVITPDNIDDRYDIFLFMIPDHLQGDFYEKYIKNSLKENS
                     CLVFAHGYSIQFGLVRPDFSSDVILVAPHGPGDDLRERFIRGSGISCYVAVQQDHSGE
                     AANIALALAKAVGSTRIGAFQTTFEHETLGDIFGEQALLCGGLAELVTKSYEALVRNG
                     IPEENAYIETAHQIDLLASLLKRHGIHGMAERISMTAQYGMLKMNDKIIDRDGEKRLN
                     QLFEKIKSGEFARDWQEEYETGFKNLKEYKKKLKNHSIEKTAEKIKKIIE"
     gene            complement(1166..1921)
                     /locus_tag="ENO07_06985"
     CDS             complement(1166..1921)
                     /locus_tag="ENO07_06985"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:PRK05265.1-3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HDS01748.1"
                     /translation="MRNRYFRIEDYAVKKLSVSLDQIGLLREARKIRTPDPVTAAGVA
                     EIAGADSISIHIRLDRRGIRERDLYLLKETCKTRLNLNISPKSEMLSMISEVKPHEVT
                     ILPERATELYTERGISFESEEEFDLDLFGQIQGTGSRLFLMVEPEAADIKAAAKAGCA
                     GIQLYSLAYTTVRTETERQVELDKIAKAAELAAKNDLEVRLGGGLDYNNIFPFLKIDE
                     VSEFVIGHAIVAQALMVGMAKAVEDMVTIVKWS"
BASE COUNT          519 a          606 c          530 g          639 t
ORIGIN      
        1 cccgatcaat ctgccgcggt cgaatgcgcc tgtgaagaaa tcgggctgtt ctttcatatg
       61 ctccgttatt ttctcccggc tgtccctgcc ggtcaggcgc acggggaggc cggaggcttt
      121 ccagacctga atcaggctgt cataatcttc cggccgcaga tttctatatg taatttccat
      181 ttattctata atcttcttta tcttttccgc cgtcttttcg atcgagtgat ttttgagttt
      241 tttcttgtat tctttgaggt tcttgaaacc agtttcatat tcttcctgcc agtcgcgggc
      301 gaactcgcct gatttaattt tttcaaatag ttgatttaac ctcttttcac catcccggtc
      361 aattattttg tcgttcattt tgagcatgcc gtactgggcg gtcatcgata tccgttcagc
      421 catgccgtga atgccgtgtc tcttaagcag cgatgccagg agatctatct ggtgtgccgt
      481 ctcgatatat gcattttcct ccgggatgcc gtttctgaca agggcctcgt atgattttgt
      541 gaccaattct gccaggcccc cgcaaagcag ggcctgctcg ccaaaaatgt ctcccagggt
      601 ttcatgttca aaggtagtct ggaaggcgcc gatgcgcgtt gagccgaccg cttttgccag
      661 ggccagggca atattggcag cctccccgga atggtcctgc tgtaccgcaa cgtagcatga
      721 aattccggaa cccctaataa atctttcgcg caaatcgtca ccgggaccgt gaggcgccac
      781 aagtatgaca tcagagctga aatccggccg gacaagcccg aactgaatag aatagccatg
      841 agcgaagaca aggcaggaat tttccttcag gctgttcttg atatattttt catagaaatc
      901 gccctggaga tggtcaggta tcataaacag gaatatatca tagcggtcat caatattatc
      961 cggcgtaatt accttcagcc cttcggcccg ggcggcattg gctgaaagac tctccagttt
     1021 aatgcagata ttcacctcaa tggctgagtc ccttagattg agagcgaaag cacgcccctg
     1081 gcttccatat ccaataatca gaatttgctt atttctgata tattttatat ccgcattctg
     1141 atctctgtag attttctgca aaacatcaac tccatttgac tatcgtaacc atgtcctcga
     1201 ccgctttggc catcccgacc atcagggcct gggcgactat tgcatgcccg atcacgaatt
     1261 cactgacctc gtcgatcttg aggaagggga agatgttatt gtagtccaga cctccgccga
     1321 gacggacctc cagatcattt ttggctgcaa gttccgccgc ctttgcaatc ttgtccagtt
     1381 caacctgcct ttcagtttcg gtcctgacgg tagtataggc caggctgtaa agctgaatac
     1441 cggcgcatcc tgctttagct gcggccttaa tatcggccgc ctccggttca accatcagaa
     1501 acagcctcga gccggtaccc tgaatctggc cgaagaggtc gagatcaaac tcctcctcgc
     1561 tctcgaagga aattccccgt tcggtgtaaa gctcggttgc tctttcggga aggatagtta
     1621 cctcatgcgg tttaacctcg ctgatcatgg aaagcatctc gcttttgggg gagatattga
     1681 gattcaggcg agttttgcag gtctctttca aaagatacag gtcgcgctcc ctgatacctc
     1741 ggcgatccaa tcggatatga atggatatgg aatcagcgcc ggcaatctcg gccactccgg
     1801 ccgcggttac aggatcgggc gtgcgaattt ttcgcgcttc ccgcaggagc cctatttgat
     1861 ccaagctgac agaaagtttt ttcacagcat agtcctctat tctaaaatat cgattgcgaa
     1921 tttattgatt ggactggtcg tcccagaaca caattctcca taaatcatcc tcacccgact
     1981 gccggaattt gaattccgcc aggccggtgg cctcgtattg ttccagcccg aagtctccgt
     2041 cagtgtctcg aaccgagagc tgaaaataga ctttgtatac ctcccaggtt tcatctccct
     2101 gaatctcggt tctgtcgaaa accgggatcc atggatcgag ctggatttca tcaaaaaatg
     2161 cgaacagatt acgtgttctt tcgatatccc ccgaggcgcc gtcacgtgga atttcaacgg
     2221 ttgcgatctc tccggcaagg tcctgatcga cataacggaa gatgaaattt tcatccaggc
     2281 agttctcata gaca
//
LOCUS       DSEB01000387            2055 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1009481, whole genome shotgun sequence.
ACCESSION   DSEB01000387 DSEB01000000
VERSION     DSEB01000387.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1009481"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..605
                     /locus_tag="ENO07_06990"
     CDS             <1..605
                     /locus_tag="ENO07_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDS01749.1"
                     /translation="TIMGLSGYRDFEGDIIFKGESIKDLDINERAKRRITLAWQEPAR
                     FEGLTIRDFIRSSARDKSVDNLKRTLDEVGLDSDEYLMRAVDKTLSGGERKKVELASI
                     LAMEPEVALLDEPDSGIDIESIERIFQAVKVLKDKGTTVIMITHSLAVLNQAEHAFLM
                     CNGTIVDKGVVSKIRSYFEDKCIPCPHKNVPRMAKTGGRV"
     gene            602..1552
                     /locus_tag="ENO07_06995"
     CDS             602..1552
                     /locus_tag="ENO07_06995"
                     /inference="COORDINATES: protein motif:HMM:PF01458.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HDS01750.1"
                     /translation="MSADLTTINDLYEKINSDPHELDDPNVAHLVIDHNKVLGQHQVP
                     GLHMDVDELEDGIKAVMRLDDNTVLEKQVHLCFGMYPEKGVQRIIMDVTIGKNAKISL
                     LSHCVFPYAIDVQHIMHGQIRVGEGSEYSYFERHVHSPKGGVKVYPNAKVEIGKRATF
                     RTEFELLRGRVGLIEIDYEATCQAESILEMNTRISGRADDVIKVREIGHLVGEHSKGV
                     LTSKIAVRENARAEVYNELTATAAYARGHVDCSEIVQDNGIVSAIPIVRVEHPKAHIT
                     HEAAIGSVDNKQLETLMSRGLSEDDAVELIISGLLSRKKK"
     gene            1578..>2055
                     /locus_tag="ENO07_07000"
     CDS             1578..>2055
                     /locus_tag="ENO07_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01751.1"
                     /translation="MSALEDFAVRILKEIAFPELSRRRRHRPRGDLRDFVRRHYDDAP
                     DGSKYRRPFLKSKGRVDVMAEEEKKIEKKIEETQEEIEKAGEGDVTMKEVDKLKKDLD
                     SLRKDLKDLTGSVKDLTVSKAAGAKEKVWRTASDIEGRATRYATDAYDTVRDQGERY"
BASE COUNT          593 a          413 c          589 g          460 t
ORIGIN      
        1 ctactattat gggcctctca ggctatcgcg attttgaagg agatatcatt tttaaaggag
       61 agtctattaa ggacctggat atcaatgagc gcgccaaaag gcgaattacc ctggcctggc
      121 aggaaccggc ccgctttgag ggcctgacaa tccgtgattt tataaggtct tctgccaggg
      181 acaaaagtgt cgacaatctc aagagaacgc tggatgaggt cggacttgat tccgatgaat
      241 atcttatgag ggccgtcgac aagaccctga gcgggggaga gcggaaaaaa gtcgaactgg
      301 cgtcgatcct ggccatggaa ccggaggtgg cgcttctgga tgagcccgat tcaggcatcg
      361 atatagaatc catcgaacga atatttcaag cggtaaaggt cctcaaggat aaaggaacaa
      421 cagtgattat gattacacat agcctggcgg tattgaatca ggccgagcac gcatttctga
      481 tgtgcaacgg cacgattgtg gacaagggcg tggtttccaa aataagaagt tactttgaag
      541 acaaatgtat tccctgtccg cataagaatg ttcccaggat ggcaaaaact ggaggtcggg
      601 tatgagcgct gatctgacaa ctattaacga cctctatgag aagataaatt cggatccgca
      661 tgagctcgac gaccccaatg tcgctcatct ggtaatagac cataacaaag tactcgggca
      721 gcatcaggtc cccggtttgc atatggatgt cgacgagctc gaggatggaa tcaaggcggt
      781 tatgcgtctg gatgacaaca cggttctgga gaagcaggtg catctctgtt tcggcatgta
      841 tcccgagaag ggcgtgcaga ggattatcat ggatgttacc ataggaaaga atgccaaaat
      901 ttctctcctg tctcattgtg tttttcctta cgccatagat gtccagcata taatgcatgg
      961 acagattcgt gtcggcgagg gatcggagta ttcctatttc gaacggcatg tacacagtcc
     1021 caagggcggg gtgaaagtat atcccaatgc gaaagtagag atcggcaaga gagcgacttt
     1081 cagaaccgaa tttgaacttc ttcgcggcag agtggggttg atagagatcg attatgaggc
     1141 cacctgccag gccgagagta ttctggagat gaatacccgg atcagtggaa gggctgatga
     1201 tgttataaaa gtgagggaga tcggacatct tgtgggtgaa cattccaaag gtgttctgac
     1261 ctcgaagata gcggtacggg aaaacgcccg ggccgaggta tacaatgaac tgacagctac
     1321 ggcggcctat gcccgcggtc atgtagattg cagcgagatc gtacaggaca acggaatagt
     1381 ctcggctata ccgatagtca gggtcgaaca tcccaaagcc catatcactc acgaggcggc
     1441 gatcgggagt gtagataaca agcaactgga aacgcttatg tcgcgcggat tgtcggagga
     1501 cgatgctgtc gagcttatta tttcgggcct cttgagcaga aagaaaaaat gatgaaggga
     1561 tggtcaggag cggttttatg agtgctttgg aggattttgc agttaggata ttaaaagaaa
     1621 tagcttttcc ggaactctcc cgccgcaggc gtcataggcc gcgcggggat ttgcgtgatt
     1681 tcgtcaggag gcattatgac gatgctcctg acggttcgaa atacaggaga ccttttttaa
     1741 aatctaaagg aagggtggat gttatggctg aagaagaaaa aaaaatcgag aaaaaaatcg
     1801 aagagaccca agaggaaata gaaaaagcag gggaaggaga tgttactatg aaagaagtgg
     1861 ataagctcaa aaaagatctg gattctttgc ggaaagactt gaaggatctg acaggttcag
     1921 tgaaagatct cacggtatca aaggccgcag gcgccaagga aaaagtctgg aggaccgcca
     1981 gcgatatcga gggtcgcgct accagatatg ctactgacgc ctatgatacg gtccgtgatc
     2041 agggcgagag gtatc
//
LOCUS       DSEB01000388            4290 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001875, whole genome shotgun sequence.
ACCESSION   DSEB01000388 DSEB01000000
VERSION     DSEB01000388.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001875"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..910)
                     /locus_tag="ENO07_07005"
     CDS             complement(<1..910)
                     /locus_tag="ENO07_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDS01752.1"
                     /translation="MLYDKKHPKSHLVSSLQERAKELNCLYRTEELMNTLDASIDSVC
                     RGIIKAIPPGWQYPDICVARITIDKDSWQSENFQETEWELSTDVKIQDKIIGKISVYY
                     TKAMPTFDYGPFLKEEKKLVATIAERLGHFIMYHKMKHMYSEWDSAKRDISRRRRGRW
                     QVVLDLLKQTDRNLYYTMSHKMLNYLCWSGISEAEELRHYTGTDRSSDDHELKADSNR
                     PHQKEAITISNELTERIFKIAADHLSDHEILDRVQKWIQEDKMGFLVQVVNRNLSLST
                     VADAIRRFHHIEDEGFELHSPSKRGVT"
     gene            complement(910..2664)
                     /locus_tag="ENO07_07010"
     CDS             complement(910..2664)
                     /locus_tag="ENO07_07010"
                     /inference="COORDINATES: protein motif:HMM:PF01326.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01753.1"
                     /translation="MSVKSYSAAGEKVPPFKKQFFESGEVITRIGTGSLGGKALGLAF
                     IKDTLASKINPSDYDNITVNIPTLAVIATDFFDRFMQINNLYDTNFEEMPDDRIAHAF
                     QNAELPPELNGDLRRLIADVHTPLAIRSSSLLEDAIYEPFAGVYGTKMIPNNQPGTDA
                     RFSKLVEAIKFVYSSVFFREARDYIRATRQSIESEKMAVIIQEVVGLRHWERYYPDIS
                     GVARSYNFYPTSHARPEDGVVDLALGLGKTIVDGGTVWSYSPAYPKSVPPYNSIGDLM
                     KLTQTKFWAINMGKPPKYDPTKETEYMIQGGLKDAELDGTLDLAASTYVPERDRISPG
                     TGPRGPRIINFAPILTVNQIPLNNLIKDLLKACEDAVGAAVETEFALTIDNKQKDRPI
                     RLGFLQVRPMVVSDAEVKVTDDELNSENALVASENVLGNGVVDDITDIVYVRPDAFNP
                     GNTQQIAADIEKLNRNLVNSGTPYVLIGFGRWGSSDPWLGINVNWAQISGSRVIVEST
                     LPNMNVELSQGSHFFHNITSFQIPYFSVRFDGKYKIRWDRLEKQKAEAETEYVRHVKL
                     ERPLNVKVDGRSGRGVIL"
     gene            complement(2796..4082)
                     /locus_tag="ENO07_07015"
     CDS             complement(2796..4082)
                     /locus_tag="ENO07_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HDS01754.1"
                     /translation="MSTGSFNPFEMAQAQFDKIAEKLDLDQAARELLREPLREYSTAI
                     PIHMDDGSVKVFRGFRVQHNDARGPCKGGIRFHPQETINTVRALAMWMTWKCAVVDIP
                     LGGAKGGVICDPHNLSLREQEQICRGWIRQIARDIGPYRDVPAPDVMTSAQHMLWMLD
                     EYETIYGEKKTGLITGKPVGMGGSLGRTEATGYGVIYTVREALKELELRPEMTRASVQ
                     GFGNVAQYAIQLYQQMGGTVVCVSCWDQEDQKSYSFKKEGGVDRDELLSVTDRFGGID
                     KNKALDLGYEVLPGEAWIEQDVDILIPAAIENQITGENVGNISEKVKVIAEGANGPTT
                     PQADKVIEERGIFLIPDFLCNAGGVTCSYFEQVQSNLNYFWEKDEVLGRLDTKMTSAF
                     VAVSELARKQKMYMRDAAYVISIGRVAQACRDRGWV"
BASE COUNT          993 a         1150 c         1010 g         1137 t
ORIGIN      
        1 ccgtcacacc gcgcttgctg ggcgaatgca gctcaaagcc ctcatcttcg atatgatgaa
       61 aacgcctgat agcgtcggcc acagttgaaa gagagaggtt acggtttacc acctgtacca
      121 gaaagcccat cttatcttcc tgaatccatt tctgcacacg gtccagtatt tcatgatcgc
      181 tcagatgatc cgccgcaatt ttaaatatgc gttcggtcag ttcattcgat attgtgatgg
      241 cctctttttg atgcggacgg tttgaatcgg ccttcagctc atggtcatcg ctggaccggt
      301 ctgttccggt atagtgacgc agctcctccg cctcagagat gccgctccag cagaggtaat
      361 tgagcatttt atgcgacatc gtgtaatata aatttcggtc ggtctgtttc aaaagatcga
      421 gcactacctg ccatcttccg cgtcttctgc gggagatatc tcttttggcg ctgtcccatt
      481 cgctatacat gtgcttcatt ttgtgataca tgatgaaatg cccgaggcgt tcagctatgg
      541 tggctaccag ctttttttcc tccttcaaaa acgggccgta atcgaaggtg ggcatggctt
      601 ttgtgtaata tacactaatt ttgccgataa tcttgtcctg aatctttacg tccgtgctta
      661 attcccattc ggtttcctgg aaattctctg actgccagga gtccttatcg attgtgatcc
      721 tggccacgca gatatcggga tattgccagc ccgggggaat ggctttaatt atgcccctgc
      781 atacggaatc gatgctggcg tcaagagtat tcattaactc ctcggtacga tagaggcagt
      841 taagctcctt ggcacgctcc tgcagggagg agaccagatg gctttttgga tgtttcttat
      901 cgtatagcat tacagaatta ctccccgacc gctccttcca tcaactttaa cattcaaagg
      961 ccgttccagt ttcacgtgcc ttacatattc tgtttcggct tcagcctttt gcttttccag
     1021 tcgatcccac ctgattttat acttaccgtc aaacctgacc gaaaaatagg gtatctggaa
     1081 actggttata ttgtggaaaa aatgtgagcc ctggctgagt tcaacattca tattgggaag
     1141 agtcgattcc acaataaccc tggagccgga aatctgggcc cagttgacat taattcccag
     1201 ccacggatcg gagctgcccc agcgtccgaa accaatcagg acatagggtg tgccggagtt
     1261 gaccagatta cgattgagtt tctcgatatc cgcggcaatc tgctgcgtat tcccggggtt
     1321 gaaggcgtcc ggcctcacat atactatgtc cgtaatatca tcaacgacgc cgttgccgag
     1381 cacattttcg gaagccacca gggcattctc ggaattaagc tcatcatctg tgaccttgac
     1441 ctcggcgtca ctcaccacca tcggcctgac ctgcaggaac ccgagcctga tcggccggtc
     1501 tttttgctta ttgtcgattg tcagcgcaaa ctcagtctca accgccgccc caacagcatc
     1561 ctcgcaagcc ttgagcaggt cctttataag gttattcaag ggaatctggt taaccgtaag
     1621 aatcggcgca aagtttatta ttctgggccc cctgggaccg gtgccggggg agatccgatc
     1681 acgctccggt acataggtgg aggccgctaa atccagagtg ccgtctagct cggcatcctt
     1741 aaggccgccc tggatcatat attcagtctc tttcgtggga tcgtattttg gcggtttgcc
     1801 catattgatc gcccagaatt tggtttgggt cagtttcatc aggtctccaa ttgaattgta
     1861 aggcggcacg ctctttggat aggctggaga atagctccag accgtaccac catcaactat
     1921 ggtcttgccc aggccgaggg ccagatctac cactccatcc tccggcctgg catggcttgt
     1981 gggataaaag ttatatgatc tggcgactcc ggagatatcg ggataatagc gctcccagtg
     2041 cctcaggccg actacttcct gaataatcac ggccatcttc tcgctctcga ttgactgcct
     2101 ggtagcgcgg atgtagtccc tggcctccct gaagaagacg gaggaatata cgaattttat
     2161 cgcttccacc agtttgctga agcgagcatc agtgccaggc tggttgttgg gaatcatctt
     2221 ggtgccatag acacccgcaa aaggctcata gattgcatcc tccagaagac tcgatgaacg
     2281 tatcgccagg ggcgtgtgga catcggcaat cagcctcctg agatcaccgt tcagctccgg
     2341 cggcagttcc gcattctgga acgcatgggc aatcctgtca tcgggcattt cctcgaagtt
     2401 tgtgtcatag agattgttga tttgcatgaa gcggtcgaag aaatctgtgg caattaccgc
     2461 cagagtggga atattgacag tgatattgtc ataatcggac gggttgattt ttgatgccag
     2521 ggtgtccttt ataaatgcca gcccgagcgc tttaccgccc agtgaaccgg ttccgattct
     2581 ggtgattacc tcacccgatt caaaaaactg tttcttgaag ggggggactt tttctcccgc
     2641 ggcactgtat gatttgacac tcattataaa cttccctgga catcgtcaaa ctcaaatacg
     2701 ccataatata tattattcat aatcagatgc aaataaaagg gaggctgacc catagaatga
     2761 gcgcctcccg aatcaattcc atattaaggc gggttttaga cccagccgcg gtctctgcag
     2821 gcctgggcca cacggccgat cgaaatgaca taggccgcat cacgcatata catcttctgc
     2881 tttcgtgcaa gctcacttac tgcgacaaag gccgaggtca tcttggtgtc aagacggccc
     2941 aggacttcat ccttctccca gaaataattc aggttgctct ggacctgttc gaaataactg
     3001 caggtcactc cgcctgcgtt gcacaggaaa tcgggaatca ggaatatgcc gcgttcctcg
     3061 atcaccttat ccgcctgagg agtggtgggg ccgttggcgc cctcggcaat gacctttacc
     3121 ttttcggaaa tattcccgac gttctcgccg gtaatctggt tctcaatcgc cgccggaatc
     3181 agtatatcga catcctgttc gatccaggcc tcccctggaa ggacttcata acccagatcg
     3241 agagctttgt tcttgtcgat ccctccaaag cggtcggtca cgctcaggag ttcatcgcga
     3301 tccaccccgc cttccttctt gaatgaatag gatttttgat cctcctggtc ccagcaggat
     3361 acgcacacaa cggtaccacc catctgttgg tagagctgga tcgcatactg ggccacatta
     3421 ccgaagccct gtacgctggc cctggtcatc tcggggcgaa gctcaagctc tttcaatgct
     3481 tcccgcaccg tatatatcac gccatagcct gtggcttctg tgcggcccaa agaacctccc
     3541 atgccgactg gtttgccggt aataaggccg gttttcttct cgccgtaaat tgtctcgtac
     3601 tcatccagca tccagagcat atgctgggcg ctggtcatca catcaggagc cggaacatca
     3661 cgatatggac cgatatcccg ggcgatctga cgaatccatc cgcggcaaat ctgttcctgc
     3721 tcgcgcaggc tcagattatg cgggtcacag atcacgccgc ctttggctcc gcccaatgga
     3781 atatcgacca cagcgcattt ccacgtcatc cacatcgcca atgctcgcac cgtgttgatg
     3841 gtctcctggg gatgaaacct gatccctccc ttgcaggggc cgcgggcatc attatgctgt
     3901 accctgaatc ccctgaaaac tttcacagag ccgtcatcca tatggatcgg gatagcggtg
     3961 gaatactctc tcaatggttc gcgaagcaat tctcgggctg cctggtccag gtcgagcttt
     4021 tcggcaatct tgtcaaattg ggcctgggcc atttcaaacg gattgaatga tcctgtagac
     4081 attttgaact aactccagtt aattttaagc atcattttaa aacaaaaagt gcaaaataaa
     4141 aagctcctcg aggagccgaa aaaatatccc ccttcttata caaacttcat aacttttttg
     4201 ttatcaataa gatgcattcc ggatgcatcc tttcacactt gcaaaaattc gttcagaata
     4261 ataggacatt gtgaaaaaaa tgtcaagctc
//
LOCUS       DSEB01000389            2392 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1006808, whole genome shotgun sequence.
ACCESSION   DSEB01000389 DSEB01000000
VERSION     DSEB01000389.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1006808"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            13..1104
                     /locus_tag="ENO07_07020"
     CDS             13..1104
                     /locus_tag="ENO07_07020"
                     /inference="COORDINATES: protein motif:HMM:PF13519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDS01755.1"
                     /translation="MQCTPTEVCLPVSGDDVDGNLVGCEVFSGPGIISDGNWCYTPMG
                     SETVEVTVKCTDECGAFCQSSFSIEFVIQENWAVLTMDRTGSMSLTNAFGESRILRAK
                     NLAHDEIDKLLDESDADYPGIFEVAIYYFNSSGGVILAQDFTSDAQTLHDAIDAIPGP
                     RHDTPLAAAMCQAHCELPELDGECNRYVITYTDGLENASTEEDICEVCVECNQYMPTG
                     WNYDCDPANPSSCTEWQLCLADKFSQSGVNLMHYFGNPINPFEKGLAAEGLEDLYFLK
                     YTAEQSDGQLIYHSDLEETCGDANADGLLNISDAIHIINYVFVAGSPAPEPLAAGDAN
                     WDGGINVTDAIFILQYIFTGTETPQCALQ"
     gene            1355..2332
                     /locus_tag="ENO07_07025"
     CDS             1355..2332
                     /locus_tag="ENO07_07025"
                     /inference="COORDINATES: protein motif:HMM:PF00494.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01756.1"
                     /translation="MELEKSYKYCLSLNHLGMEYPLLAASLLPEDKKNSIAATLASFR
                     VIIDKIHSSLGSSSLDRLNKDEIRGMIEEWNSMVRDARQGKCRDHEVCLALADSFNKF
                     NIPTHPWEDFSNALNFHLDNVSVADEKTFQDYCRWTYGAGVYNYIHILASQNNGPGYT
                     LNFKPEFLSDDLAALFFITHTLTDIAHDLTSTRDGFIFIPRTVLERHGISRGDIMNYY
                     RRGEIDNRFRKMIDEYYHLGREYELFVREKLVLIRGEIQKEDWFILDLLLNIYSNFLH
                     RIWEYPEAIFRGGFPIDPAMVFINAMKLQKDLGLNFRRDLSRLLSQSVA"
BASE COUNT          610 a          549 c          599 g          634 t
ORIGIN      
        1 agcggggtga taatgcagtg cacgccgaca gaagtctgtc tgccggtttc gggtgatgac
       61 gttgacggaa atcttgtcgg atgtgaggtt ttttccggac cgggtataat ttccgatgga
      121 aattggtgct acactcctat gggatcagag acagtcgagg tgaccgtaaa gtgtactgac
      181 gaatgcggcg ccttctgcca gtcatcattc agcatcgaat ttgtcatcca ggaaaactgg
      241 gccgtgctca ctatggaccg cacaggctcc atgtccctca caaatgcttt cggcgagtcc
      301 aggattttaa gggccaagaa cctggctcat gatgaaatcg ataaactgtt ggatgaaagt
      361 gatgccgatt atcccggcat cttcgaagtg gccatatact atttcaattc ctccggtggc
      421 gtgattctgg ctcaagattt cacctctgat gcccagacct tacatgatgc catcgatgcc
      481 atacccggac cgcggcatga tactccgctg gcggctgcga tgtgccaggc tcattgcgaa
      541 ttaccggagc tggatggtga atgtaacagg tatgtgataa catataccga cggtcttgaa
      601 aatgccagca cagaggagga tatctgcgag gtctgtgtgg aatgcaacca atatatgccg
      661 accggatgga attatgactg tgatcccgct aatccttcca gctgtaccga atggcagctc
      721 tgtctggcgg ataaattctc ccagagtggt gtcaatttga tgcactattt cggtaatccg
      781 atcaatccat tcgaaaaggg attggccgcc gagggcctgg aagatctgta cttcctgaaa
      841 tataccgctg agcagagcga cgggcagttg atctatcatt ccgatctgga ggagacctgc
      901 ggtgatgcca atgctgacgg cctgttgaat atcagcgatg cgattcatat tattaactat
      961 gtctttgtgg ctggaagccc tgcccctgaa ccgctggctg caggtgatgc caactgggat
     1021 ggtgggatta atgttaccga cgctatattc atattgcagt acatatttac aggaaccgaa
     1081 accccgcaat gcgcgttgca atagccaaaa tagatccttg ataataatcg cccgctttta
     1141 agagcgggcg tttttttatg tggatatatg tttcaacatt tattatgctg gtaatcagga
     1201 tgatgtgaaa aatagaggtg tgaacgcctc tcaggcaatg cacaaatatt tagattcagg
     1261 ctgcgggggt gcaaaaaatt tgaatatgga ttaaaggcat ccaatataat tgtttttcct
     1321 ttcgtatggt tgttataaat cagggagatg acacatggaa ttggaaaaga gttataaata
     1381 ctgcctgtcg ttaaatcatc tgggaatgga atatcccctt ctggccgcaa gccttctgcc
     1441 cgaagataag aaaaattcaa ttgcggctac acttgcatct ttccgggtaa taatagacaa
     1501 aatccacagt tccctgggga gcagcagcct cgaccgcctc aacaaagatg aaatcagggg
     1561 catgattgag gagtggaatt cgatggtgcg ggatgcgcgc cagggtaaat gccgcgatca
     1621 tgaagtctgc ctggcgctgg ctgactcatt taataagttc aatatcccga cccacccctg
     1681 ggaggatttc agcaatgccc tgaatttcca tctggataat gtcagcgttg ccgatgagaa
     1741 aacattccag gactattgcc gctggacata tggcgccggt gtttataact acatccacat
     1801 ccttgcttct cagaataacg ggccgggcta taccttgaat ttcaagccgg aatttctctc
     1861 cgatgatctt gccgcgctgt ttttcattac tcataccctg accgatatcg cgcatgacct
     1921 gaccagtacc agggatggct ttatcttcat cccgcgcact gttctggagc ggcatggcat
     1981 atcacgcgga gacataatga attattacag gcgaggcgag atcgataacc gtttccggaa
     2041 aatgatcgat gaatattatc acctgggccg ggaatacgag ctgtttgtgc gtgagaagct
     2101 ggtgctcatc aggggcgaaa tccagaaaga ggactggttc atactcgatc tgcttttgaa
     2161 tatatactcc aatttcctgc acaggatctg ggaatacccc gaagccatct tccgcggcgg
     2221 cttcccgatc gatccggcga tggtctttat caacgccatg aaactgcaga aagacctggg
     2281 cctgaacttc aggcgggatt tatccaggct gctctcacag tctgtagcct gacccattat
     2341 tattttcatt attaatattt ttgatgattt ttgcacccaa gttgtctatc ta
//
LOCUS       DSEB01000390            2655 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1005389, whole genome shotgun sequence.
ACCESSION   DSEB01000390 DSEB01000000
VERSION     DSEB01000390.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1005389"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(130..2247)
                     /locus_tag="ENO07_07030"
     CDS             complement(130..2247)
                     /locus_tag="ENO07_07030"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00403.24,HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDS01757.1"
                     /translation="MNREIELKIVDLDCPDCAASLEKGISRLKGVKNCSLEFATGKIN
                     LSIDNQIIDREKVIKEISRLGHVAVDKSAGGHEVSETKGRGSLARLKDILLMVAVTAM
                     LIEIALSVFDQPGYLQIIFSVTAILTGGLFIFRSAFYALKNLTADMNLLMSLAVIGAV
                     IIGEWSEAAVTIVLFSIANYLEGRSVRKAHRAFKSLTEQIPHEIEMETADGPQRVRLE
                     DLKQGDIILLKPGMTCPTDCEIAEGTAYINQAAVTGESMPVSKTEGDKLLGGSINTDG
                     FLKARVLKPFSDSTLTRIMHMVEEAASRKSRLASIVDRFAKVYTPIVVGIAILVFAIP
                     VLFFHGAFETWFYRALVFLVISCPCALVISTPVAVICGLTRSARGGILIKGGVFLEKL
                     ANVRAVLFDKTGTLTEGRFGVVHVVSLDGHSSDNIIALAASVEQFSEHPIAGAILEHA
                     RSMKIKPFESRDFRAFPGEGARAQIEGSQYTIGSHRFFHDSDITDVELHKKVTDFEDK
                     GYSLVLLARDDRLIGAIALADMAKSESAEAIAELYQSGISESVMLTGDNARTAESIAE
                     QLGIRHVESQLLPEEKSKLVQKYREKYGLVAMVGDGINDAPALALADVGIAMGTGGSD
                     LAIEAADVAIIGDNPKMVPKSIKLSRRTLGIIKFNIVFSLLVKFIFMILAGLGLATLW
                     MAVFADMGTSLLVIANSLRLLQK"
     gene            complement(2247..2591)
                     /locus_tag="ENO07_07035"
     CDS             complement(2247..2591)
                     /locus_tag="ENO07_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDS01758.1"
                     /translation="MGSPNKKEVKYQTNGCYSPYDIESIFRLSEAFKVLGDATKLRIC
                     ILLSERELPVSGIAAELDLSDSAASHSLRILRNLRLVKFRREGKMIYYSLDDDHVKEL
                     IKVGYDHILEEK"
BASE COUNT          686 a          760 c          570 g          639 t
ORIGIN      
        1 aaatgataac tctttcagga atgtgataat tttgtaaatt tatattcaaa atcgcgccag
       61 ttcaatcatt ttttccaaaa tttctggacc ggcagccaag acacagggca aaaaagatct
      121 ctgcaccgcc tacttctgta agagtcgcaa tgagttagca atcaccagga gggatgtgcc
      181 catgtcggca aaaaccgcca tccagagcgt agcgagaccc aaaccggcca gaatcatgaa
      241 gatgaacttg accagaagtg aaaagacaat attgaatttg ataatgccaa gcgtcctgcg
      301 cgagagcttg atcgatttcg gcaccatctt aggattatcc ccgatgatgg ctacgtcagc
      361 cgcctcaatc gccagatccg aaccacccgt gcccatggca atgccaacat ctgccaatgc
      421 cagcgccgga gcatcattga taccatcccc taccatagca accaagccgt atttctcgcg
      481 atatttctgc accagcttac tcttctcctc cggaaggagc tgactttcta catgacggat
      541 acccagctgt tccgcaatag actcggcagt acgggcattg tccccggtaa gcataacaga
      601 ttccgatatt ccgctctgat ataattcagc tatggcctct gcggactcag attttgccat
      661 atccgccagt gcgatagcgc cgatcagcct gtcatccctt gccagcagca ccagcgagta
      721 acctttgtcc tcaaaatcag tcaccttctt atgaagttca acatcagtga tatcgctgtc
      781 gtgaaaaaat cggtggcttc cgattgtgta ctgcgaaccc tctatttgcg ccctggcgcc
      841 ctcgccggga aaagccctga aatccctgct ttcaaatggc ttgatcttca tgctgcgggc
      901 atgctcgagg atcgcccccg cgatcggatg ctctgaaaac tgctccactg aagcagccag
      961 cgcaataata ttatccgaag aatgcccatc cagcgagacc acatgtacca cgccaaaacg
     1021 tccctcggtc agggtcccgg tcttgtcgaa aagcacagcc ctgacatttg ccagcttctc
     1081 cagaaacact ccgcccttta tcaagatgcc gccgcgtgct gaacgagtca ggccgcaaat
     1141 taccgctacc ggggttgaga tcacaagggc gcaggggcag gatataacca aaaatactag
     1201 cgcgcggtag aaccaggtct cgaatgcgcc gtggaagaat agtaccggaa tagcaaacac
     1261 caggattgca atgcccacca cgatcggagt atagactttt gcaaatctgt ccacgatact
     1321 cgccagcctg gacttgcgtg aggccgcctc ctccaccata tgcataatgc gggtcagggt
     1381 cgaatccgaa aacggcttca ataccctggc tttaaggaag ccatcggtat tgatcgagcc
     1441 gcccaggagc ttgtcgccct cggtcttcga cactggcata gattcgcccg taaccgcggc
     1501 ctgattgata taggccgttc cctcggcaat ctcgcaatcg gtgggacagg tcatgcccgg
     1561 cttcagcagg ataatatccc cctgcttcag gtcctcgagc cgcactctct gcggaccatc
     1621 ggcggtctcc atctcgatct catgcggtat ctgctcggtc aacgatttaa aagctctgtg
     1681 tgccttacgt accgatcttc cctcgaggta attggcaatc gaaaacagca ctatcgtaac
     1741 cgcagcctcg ctccattccc cgataatcac ggcgccgatt acagccaggc tcatgagaag
     1801 attcatgtcg gcggtcagat tcttcaaggc atagaacgcc gaacggaaaa taaacagccc
     1861 gcctgtaaga attgcggtca cggaaaatat tatctgtaag taaccgggct gatcgaacac
     1921 tgaaagtgcg atttcaatca gcatcgcggt cactgccacc atcagaagaa tatccttcag
     1981 cctcgctaac gatccgcgtc ccttcgtttc tgaaacctca tggcccccgg cggacttatc
     2041 aaccgccaca tgtcccaggc gggaaatctc ctttattact ttttcacggt caattatctg
     2101 attgtctatc gacaaattta ttttgcctgt ggcaaattcc aaactgcaat ttttcacacc
     2161 tttcaggcgc gaaatcccct tctccaggct ggcagcacaa tccggacagt ccagatcaac
     2221 tattttaagt tctatctctc tattcattat ttttcttcca gaatatgatc atagcccact
     2281 ttgataagct ccttgacatg atcgtcgtcc agagaataat agatcatctt tccctcccga
     2341 cgaaatttca ccaggcgaag attgcgcaga atccgcaagc tgtgcgaagc cgcagagtcc
     2401 gaaagatcca gttctgcagc tattccggat accggcagtt ctctctcgga caataaaata
     2461 caaatcctga gcttggtggc atccccgagc accttgaacg cctcggaaag cctgaaaatg
     2521 ctctcgatat catacgggct gtaacatcca ttagtctgat acttgacttc ttttttattc
     2581 ggtgatccca taatttcgcc tcacagctta aacatcaact atgaacaatt gttcatatat
     2641 atactatatt tcggc
//
LOCUS       DSEB01000391            7131 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000619, whole genome shotgun sequence.
ACCESSION   DSEB01000391 DSEB01000000
VERSION     DSEB01000391.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7131)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7131
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000619"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..881
                     /locus_tag="ENO07_07040"
     CDS             <1..881
                     /locus_tag="ENO07_07040"
                     /inference="COORDINATES: protein motif:HMM:PF14052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01759.1"
                     /translation="PAFNQFSAEYSRRWLAFQFWAAQLDPFFDAEGDKLNRFFSVHRL
                     AIKPYKRLEIGLSETVLYARENAGWDLSYLIPILPFYWEQYNNRIDDNIYWDIDFTWY
                     PVDGLKLYGEWLIDDFQIDFVSEPHQIGFNLGVSELGLLFSQYLQLEFDYTQIRNTVY
                     GQNKFYNVYTHEGVIIGSSLGTDSDRLRYAATYHANPFLRISAGGEYRRKGEGRYNTG
                     EYPAPKGQKFPSGIVEEEWDNYLRLDILKGTTIEGQVTAGYKSIENLDNIEGNCLDSP
                     YLSVNILYHFKRAFLF"
     gene            943..1974
                     /locus_tag="ENO07_07045"
     CDS             943..1974
                     /locus_tag="ENO07_07045"
                     /inference="COORDINATES: protein motif:HMM:PF10300.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3808 domain-containing protein"
                     /protein_id="HDS01760.1"
                     /translation="MRIILNTTIALAALISPVLADFPDSSDVGLIISGIELTLNEEYS
                     QARELYSELQTKYPRHPGPPFFIAATYQAEMKDHEDFEYEQEFVNNIELSIDLADKLR
                     NEDEDDAWAYYFMGASNLYWALFEGRKDGKWSVAHKGIRGKNLMEKCLGIDSTMYDAL
                     AGLGSYRYWGDVKGGIFAKLPFVGGDRRQGIEDLRIAAEKSIFSGDLAQSALIYVYIE
                     EGKYDLADSLAVGMRKKYPHSKSFLWARAFANLEAERYESALEFFDSLKAIIEQENQS
                     SKYNLVQISYNKMLCHYNMERYADALIEIDYCENMLLAHDVRDRLDDIFDKLNDHKKK
                     IRKILSSDG"
     gene            complement(2170..2700)
                     /gene="lspA"
                     /locus_tag="ENO07_07050"
     CDS             complement(2170..2700)
                     /gene="lspA"
                     /locus_tag="ENO07_07050"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDS01761.1"
                     /translation="MRRRLISYLALTVSIALIDIISKLIIQKNFALHESHQVLGDFFK
                     ITYILNAGGVFGSQLGSNIFYLVSAFIVVLLVVFFLFREFGKNKYIDLSLFIVLGGAI
                     GNLFDRIRMGAVVDFLDFDFLKIQLLGYKFDRWPTFNIADAAVTIGMILLVATVLFGL
                     GKTKEPEPDADHINTE"
     gene            complement(2743..3123)
                     /locus_tag="ENO07_07055"
     CDS             complement(2743..3123)
                     /locus_tag="ENO07_07055"
                     /inference="COORDINATES: protein motif:HMM:PF01258.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HDS01762.1"
                     /translation="MRARELKMYEKLLLERRAAIFQEVERMSSAARQATQREASGELS
                     SHTFHMADRGTDQSEREKVFMLSSKGKRYLFHIDEALRRIKDKTYGKCHQCGKDISPE
                     RLKAVPHARYCIECKEAEEKARQR"
     gene            complement(3157..6312)
                     /locus_tag="ENO07_07060"
     CDS             complement(3157..6312)
                     /locus_tag="ENO07_07060"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDS01763.1"
                     /translation="MLDELKGDFSHPKLEEEIIEHWKKHGIFKKVQDSRAGRPEYIFY
                     EGPPTANGRPGVHHIISRTLKDFVCRYKTMRGYHVERKAGWDTHGLPVEIEVEKKLNL
                     KNKSEVEAYGIDRFNKACRESVFTYKKEWDELTERIGYWLDLENPYLTCENDYIESVW
                     HILAKFFDDGLLYKGHKILPYCPRCETGLSSHEVAQGYKEVSDPSIFIKVRLKGQAGV
                     YFLVWTTTPWTLISNVALAVHPQADYIRVKFGEDELILAEALANRVLNDEFEILQRYK
                     GSELELWEYEPLFPYYKDGLDKAYFVTEADFVTLEDGSGIVHIAPAFGADDYNVGQKY
                     GLPVLQAVETNGEFKAEVTDYAGMFIKDADPLITKNLKERGLLFKKEKYVHNYPHCWR
                     CKSPLVYYARESWYIRTSQFKDKLLENNRTINWVPPEIGSGRFGEWLENNVDWALSRE
                     HYWGTPLPVWIGEKTGKQIAVKSVAELKEKGENVPDDIDLHKPHVDNIRIWNDEDQEW
                     MYRTPEVIDVWFDSGAMPFAQYHYPFEHSENWDKYFPADFISEAVDQTRGWFYSLVAI
                     STLYAGVAPFKNIIVCGHILDVEGKKMSKSLGNAADPWEILAEYGADALRWYLVTINN
                     PWLPIRFDTEALAEVNRKVLGTLKNTYTFFAIYANIDKLEQRAKEDGKTLGDFLDSKA
                     GEHTEIDRWVISRLNSLIKEVGELLEKYNLTAANRKIGEFIVDELSNWYVRRSRRRFW
                     AGADDPDKFRAFATLHECLLKVSRLIAPIMPFFSEKIYIELSGDGVESVHIEDYPAAD
                     ESLIEPELEKKMASAITIVSLARTARTRSRLKIRQPLSEMIVVLPSEVRPESLYNITG
                     VISEEINIKRIRFSSDDSEIISLKAKPNFKLLGPKLGKKIGMAKSAIEKLEDDVLREF
                     MTTEKLVLKLDDEQFILTGEELEIERVSKENYAVESDDGFSVAVSTLLDNGLIQEGYA
                     REVVNKIQNMRKSADFQVTDRIRVSVKSTAEVKTALDNFGDYVKGETLADELLSGDID
                     GEVRQEWDINGQPATITIART"
     gene            complement(6327..7127)
                     /locus_tag="ENO07_07065"
     CDS             complement(6327..7127)
                     /locus_tag="ENO07_07065"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDS01764.1"
                     /translation="MEEAVADIRKVTDSKPKIGIILGSGLGGLADKMDTEDTVGYENI
                     SHFPLSTVEGHAGRLLFGKLSGKDVVAMQGRFHFYEGYTMKQVTFPVRVLKALGIEIL
                     IVSNACGGLNPQFKAGDLMIITDHINMQPSNPLIGWNDPELGDRFPDMSEPYDKKLIQ
                     LAEECAIDLKLKVQKGVYVSVPGPNLETAAEYRFLRTIGADVVGMSTVPEVIVAVHQK
                     TRVLGLSIVTDMGLPDALKPTSHQIILKNAGIAEPKLTAIAEAVVAKL"
BASE COUNT         1734 a         1838 c         1693 g         1866 t
ORIGIN      
        1 ctcccgcctt caatcaattc tcggccgaat attccagaag atggcttgct tttcaattct
       61 gggcggcaca gctcgatcca tttttcgatg ctgaaggcga caaactgaac aggttctttt
      121 ccgttcacag gcttgcgatc aaaccctata aacggctcga aataggactt tccgaaactg
      181 tgctgtacgc ccgtgaaaat gccggatggg atctttcgta tctcattccg atcctgccct
      241 tctattggga gcaatataat aaccgcatcg acgacaatat ttactgggat atagatttta
      301 catggtaccc cgttgacggt ctgaaattgt acggtgaatg gctgatcgat gacttccaga
      361 tagacttcgt ctccgaaccg catcagatcg gcttcaacct gggagtttcc gaactgggac
      421 tgctgttctc acaatatctt cagcttgaat ttgattacac tcagatcaga aatactgttt
      481 acggccagaa taaattctat aacgtttaca ctcacgaggg agtgataatc ggctcatccc
      541 tgggaaccga ctccgaccgt ctcagatatg ccgcgactta ccatgccaat ccttttctgc
      601 ggatctcggc cggcggcgaa taccgccgca agggcgaggg acgttataac accggcgagt
      661 atcctgcgcc aaagggccag aaatttccat ccgggattgt ggaagaagaa tgggacaatt
      721 acctgcgcct cgatatcctc aagggtacga caattgaagg gcaggtgaca gcaggataca
      781 aatccataga aaatctcgat aatatagaag gtaactgcct ggattcgccg tatctttcgg
      841 ttaacatttt atatcatttc aagcgggcat tcttgttcta aatctgtata atgaaaaatg
      901 accctttgaa aggatagata atctcttttt tttaagcgtc ctatgagaat tattttgaat
      961 acaacaattg cacttgctgc gttgatttca ccagtcctgg ctgattttcc tgattccagt
     1021 gatgtcggtt taataatctc gggtatagaa ttgactttga atgaggaata ctctcaggcc
     1081 cgcgagttgt attcagaact ccagacaaaa tatccccggc atcccggccc gccgtttttc
     1141 atagcggcta cataccaggc cgaaatgaaa gatcacgagg acttcgaata tgagcaggaa
     1201 tttgtgaata atattgagct ctcgattgat ctggcagaca agctccgcaa tgaagatgag
     1261 gatgatgcct gggcctatta ttttatgggc gcctccaatc tgtactgggc tctttttgag
     1321 ggtcgcaaag atggcaaatg gtcggtggca cataagggga tacgcggcaa aaatcttatg
     1381 gaaaaatgcc tgggtatcga ctcgactatg tatgacgccc tggccggtct gggttcatac
     1441 cgctactggg gggatgtcaa gggcggtatc ttcgccaaac ttccctttgt cggcggagac
     1501 cgaaggcagg ggattgagga cctcaggatc gccgcagaaa aatctatttt ctcgggggat
     1561 ctggcccaaa gcgccttgat ctatgtttac atagaggagg gcaaatatga tcttgccgac
     1621 tccctggctg tggggatgcg taaaaaatat ccgcacagta agtcctttct ctgggccaga
     1681 gcatttgcca atcttgaagc agaaagatat gaaagcgctt tggaattctt cgacagcctg
     1741 aaagccataa ttgaacaaga aaatcaatcc agcaaatata acctggtgca gatttcatat
     1801 aataagatgc tctgtcatta taatatggaa agatatgctg acgccctgat cgaaattgat
     1861 tattgtgaaa acatgctatt agcccatgat gtacgggatc gtctcgatga tatttttgat
     1921 aaactcaatg accacaagaa gaaaatccgg aagattctca gtagtgatgg ataaaaaaaa
     1981 ccggtcagaa tgccacaggg gtataaaggt gagcttcaat tagggaatca agcggcagga
     2041 ttctgaccgg tatagcccac atataaaatg ataaattttt agacgcccga gcttggcccc
     2101 gctattattc atggttcatt tctatctcaa cggattagat tttcccccgg taaacgaaaa
     2161 ctgagctgct tactctgtat taatatgatc ggcatccggc tccggctctt tagtttttcc
     2221 aaggccgaat agcacggttg ccacaaggag gatcatgccg atcgtgactg cggcgtcagc
     2281 gatgttgaag gtcggccagc ggtcgaattt gtaaccgagc agttgtattt taagaaaatc
     2341 gaagtcaagg aaatccacca ccgcacccat cctgatacgg tcaaagagat tgccgatagc
     2401 cccgcccagc actataaaga gcgaaagatc gatatatttg ttcttcccga actccctgaa
     2461 caaaaagaat accaccagca gcaccacaat aaatgcggat acaagataga agatatttga
     2521 tcccagctgg cttccgaaga ccccgccggc attgaggatg taggttattt tgaagaaatc
     2581 ccccagaacc tggtgagatt catgcagggc gaaatttttt tgaatgatga gctttgagat
     2641 tatatcaatc agggcaatgg agacagttaa ggcgaggtag gatattaatc tgcggcgcat
     2701 atatttaacc ggtatcttcc ttgtttgctg ccaatagctg tattatcgct gcctggcttt
     2761 ttcttcggct tccttgcatt cgatacaata gcgtgcgtga ggcactgcct tcaggcgttc
     2821 aggggaaata tccttaccac actgatggca ttttccgtag gttttatcct tgattctccg
     2881 caatgcttca tcgatatgga agagataacg tttgcccttg gaggagagca tgaagacctt
     2941 ctcccgttcg ctctgatctg tacctctgtc ggccatgtgg aatgtatggc tggaaagctc
     3001 cccgctggcc tcgcgctggg tagcctgccg ggccgcgctg gacattcttt cgacttcctg
     3061 aaaaatcgct gcacgccttt ccagaagcaa tttttcatac atcttcagct ccctggcacg
     3121 catcttatgc tcctatgaat attgttgcgc ccgagcctaa gtccgggcga tcgttatggt
     3181 cgcaggctga ccgttaatat cccactcctg ccgcacttcg ccgtcaatat ctccggacaa
     3241 caattcatcc gccagggttt cgcccttgac ataatcgcca aaattatcga gggcggtttt
     3301 tacctctgcg gtcgatttga ccgatacccg aattctgtct gtgacctgaa aatcagcgct
     3361 cttgcgcata ttctggatct tgttcacgac ttcgcgcgca tacccttcct gaatcaggcc
     3421 gttatccagg agtgtcgata ccgccacact gaaaccatca tccgattcaa cagcgtaatt
     3481 ctctttgctg acccgctcta tctcaagctc ctcgccggtc aggataaact gctcgtcatc
     3541 cagtttcaaa acaagcttct cggtggtcat aaactcacgc aggacatcat cctcgagctt
     3601 ctctatagcc gacttggcca tcccgatctt tttgcccagc ttgggcccca ggagcttaaa
     3661 attaggcttg gctttgagag atataatttc ggaatcatcc gagctaaaac gaatcctctt
     3721 gatattgatc tcctccgaaa tcacaccggt gatattgtaa agactctccg gcctgacctc
     3781 cgacggaagc accacgatca tttccgaaag cggctgacgg attttcaggc ggctcctggt
     3841 tctggcggtt ctggccaggg acactatcgt gatagccgag gccatcttct tttccagctc
     3901 cggctcgatc aggctttcat cggcggcggg ataatcctct atatgaaccg attcaactcc
     3961 gtcaccggag agctcgatat atatcttctc tgagaagaag ggcataatcg gcgctatcag
     4021 cctggaaacc ttcaaaaggc attcgtgcaa tgtcgcaaag gcgcggaatt tatccggatc
     4081 atcagcgccg gcccaaaaac gccttctcga tctgcgtaca taccagttgg agagttcatc
     4141 aacgataaac tcgccgattt tgcggttggc ggcggtaaga ttgtattttt ccagcagttc
     4201 gccgacttct ttgatcaacg aattcaggcg cgaaattacc cagcggtcga tttcggtatg
     4261 ctcaccggcc ttcgagtcca gaaaatctcc cagggtcttc ccgtcttctt ttgctctctg
     4321 ctcaagttta tcgatattgg catagatcgc aaagaaggtg tatgtatttt tcagggtacc
     4381 gagcactttg cggtttacct cggccagtgc ctcggtatcg aagcggatcg gaagccaggg
     4441 attattaata gtaaccagat accatctcag ggcatcggcg ccgtactccg ccagtatctc
     4501 ccacgggtct gcggcattgc ccagcgactt ggacatcttc ttgccttcga catcaagaat
     4561 atgtccgcac acaataatat tcttgaaggg ggccactccg gcataaagcg tggagattgc
     4621 caccagagag taaaaccacc cgcgtgtctg atccaccgct tcggagatga aatcagccgg
     4681 aaaatatttg tcccagtttt cgctgtgctc aaaggggtaa tggtattggg caaagggcat
     4741 cgcgcccgaa tcaaaccaga catcgattac ctcgggagtg cgatacatcc attcctggtc
     4801 ctcgtcgttc cagatgcgta tattgtcgac atgcggttta tgcaggtcga tatcatcggg
     4861 cacattctca cccttttcct tcaattcagc cacggatttg accgctatct gtttgccggt
     4921 cttttcaccg atccagaccg gaagaggcgt gccccagtag tgctcgcggg agagagccca
     4981 gtcgacatta ttttcaagcc attcaccgaa ccgtccagag ccgatctccg gcggaaccca
     5041 gtttatcgtg cggttgtttt ccagaagttt gtccttgaat tggctggtac ggatatacca
     5101 ggattcgcgg gcgtaataaa ccagcggcga tttacagcgc cagcaatgcg gataattgtg
     5161 aacgtacttc tcttttttga agagcaggcc ccgttccttg aggttcttcg tgatcagggg
     5221 atcggcatcc ttgataaaca tgccggcata gtcagtgacc tcagctttga actcgccgtt
     5281 ggtttccaca gcctgaagaa caggcaggcc gtatttctgc ccgacattgt agtcatcagc
     5341 gccgaaggcc ggggctatgt ggacgattcc ggaaccgtcc tccagcgtga caaaatcggc
     5401 ctcggtcacg aaataagctt tgtccagccc gtctttatag taggggaaaa gcggttcata
     5461 ttcccagagc tccagctcac tgcctttata tctctgcaga atctcgaact catcatttaa
     5521 gacgcgattg gccagggcct cggccagaat taactcgtcc tctccgaatt taacgcggat
     5581 gtaatctgcc tggggatgca ccgccagcgc cacattggag atcagcgtcc agggagtggt
     5641 cgtccagacc agaaaataga ctcccgcctg tcccttgagt ctgaccttta tgaatatgga
     5701 ggggtcggag acctccttgt aaccctgcgc gacctcatgt gaggacaggc cggtctcgca
     5761 gcgcggacaa tagggaagaa tcttgtgtcc tttgtacaaa agcccgtcat caaaaaactt
     5821 tgccaggata tgccagacgg attcgatata atcattttcg caggtgagat agggattttc
     5881 caggtccagc cagtagccga tccgttcggt cagctcatcc cactccttct tgtatgtaaa
     5941 caccgattcg cggcaggcct tgttgaacct gtcgatacca taagcctcga cctcggattt
     6001 gtttttcagg ttgagctttt tctcgacctc gatttccacc gggaggccgt gagtatccca
     6061 gcccgccttg cgctccacgt gatatccgcg catggtttta taccggcaga cgaaatcctt
     6121 gagggtacgc gagattatat gatgcactcc gggacgtccg ttggcggtgg gaggcccctc
     6181 gtagaagata tactccggcc ttccggcacg ggagtcctgc accttcttga aaataccgtg
     6241 cttcttccag tgttcgatta tctcttcttc aagctttgga tgtgaaaaat cacccttcaa
     6301 ttcgtctaac atatcatcac ctgaatctat aatttagcga ctaccgcctc agcaatggcc
     6361 gttaatttcg gttccgcaat tcctgcattc ttcagtatta tctggtggct ggtcggtttg
     6421 agcgcatcgg gaaggcccat atctgtcact atcgacagtc ccagcacacg cgtcttttga
     6481 tgcactgcca cgatcacttc cgggacagtt gacataccga ccacatctgc gccgattgta
     6541 cgcaggaaac gatattcggc cgcagtttcc aggtttggac ccggtacaga aacataaacg
     6601 cccttctgca ccttgagttt cagatcaatt gcgcattctt cggccagctg gatcaatttt
     6661 ttgtcgtacg gctcgctcat atccgggaac cgatccccca gctccgggtc gttccagccg
     6721 atcagcgggt tggaaggctg catgtttatg tgatcggtta tgatcatcag gtccccggcc
     6781 ttgaactgag gattcagccc gccgcaggca ttggacacga tcaggatctc gattcccagc
     6841 gccttcagca cacgtaccgg aaatgtcacc tgtttcatgg tgtagccctc gtagaaatga
     6901 aagcgcccct gcatggcgac cacatccttg cctgacagct tgccgaaaag cagtcggccg
     6961 gcatgaccct ccactgtgga gagcggaaaa tggctgatat tctcgtagcc gacagtgtcc
     7021 tcggtatcca tcttatccgc cagaccgccc aggccggagc ccagtattat tccaattttc
     7081 ggtttcgaat ctgtgacttt tcttatatct gctacggcct cttcaatatg c
//
LOCUS       DSEB01000392            2294 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1007471, whole genome shotgun sequence.
ACCESSION   DSEB01000392 DSEB01000000
VERSION     DSEB01000392.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1007471"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            231..713
                     /locus_tag="ENO07_07070"
     CDS             231..713
                     /locus_tag="ENO07_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01765.1"
                     /translation="MNNTCHDSLIDALTQFEQSMAQLYVIFGRLFPSESCRWADLAAE
                     ECRQAQWLANFKIYLRNEIICQSETDIVIRRVNLTLEFIENQIRRAASNNIDLREAIL
                     VALVIEDSAFESSFLLTFNFKTPKVRKIRRKLIEATGIQKDKLVMWLDHIEGRMKMVA
                     "
     gene            complement(772..1977)
                     /locus_tag="ENO07_07075"
     CDS             complement(772..1977)
                     /locus_tag="ENO07_07075"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00072.22,HMM:PF00512.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDS01766.1"
                     /translation="MHKKSKTESGRRHRFESQMRQVQKMEAIGALAGGIAHDFNNILA
                     IIQLNAAMALDSQKTGESAEEELRQILEATARAKELTMKILMFSRKGERSDSVLCLQD
                     VVNDAMKMIRAVVPTSIDIEMNIDQNCSPVRIDTMDINQVMLNLCVNASQAMEEKGGL
                     TIGLAEVELTEDEIEEYRQRQPGFYVRLTVSDTGCGMDAETKESIFEPFFTTKPRGVG
                     TGMGLSVVYGIVESCGGIIRVESEVGAGTTFTLFFPVSDLPDLEKSVVVEEELDGSGC
                     ERILFVDDEEELASLAKRVLEKRGYRVSFFSGSNEALMAFKAAPQDFDLVVTDHNMPE
                     LTGVELAREILNIRRELPIILSTGYTSKVSTRNFRDYGFCQLLVKPYNPMALVGAVRR
                     ALDTAFRSR"
BASE COUNT          597 a          603 c          501 g          593 t
ORIGIN      
        1 gatacagcgc tactccatac aggcaagccg gtcaccggca aatttcatga tgaaagcgcc
       61 caggcgctat acgaaataaa ccggaccccc tactacgatt tggacgaccg gaccattatc
      121 ggcagtgtct atattgccag ggatataacc aatcggtagt cggcggaagg tgaaggagaa
      181 aaaatgcatg tttttctgat caacccacaa acaatacgaa ggaacaaaca atgaacaaca
      241 cgtgccacga cagccttatc gatgcgttaa ctcaatttga gcaatccatg gctcagttat
      301 atgttatttt cggcaggtta tttccgtcgg aaagctgcag gtgggcggat ttagccgcag
      361 aagaatgccg gcaagcccaa tggctcgcaa attttaaaat atacctcaga aacgagataa
      421 tttgccagag tgaaacagat attgttatac gaagagtaaa cctgacgctg gaatttatcg
      481 aaaatcagat tcgccgggcg gcaagcaaca acattgactt gcgggaagcg atcctggtgg
      541 ctctggtaat cgaggattcc gcctttgaat cttcgttttt acttacattt aatttcaaaa
      601 caccaaaagt tagaaaaata agacgcaagc tgattgaagc caccggaatc cagaaagata
      661 agcttgtcat gtggctggac catatcgagg gcagaatgaa aatggttgcc tgacgaatct
      721 gaaccggagt attgagaaag aaatatgcct ccgcccaagc ctgtgataag gtcagcggct
      781 gcgaaaggct gtgtccagcg cccttcgaac cgcccccacc agagccatgg ggttgtacgg
      841 cttgaccagc agttgacaaa aaccatagtc cctgaaattc ctggtcgaaa ccttgctggt
      901 atatccggtg gagagaatga tcgggagttc gcgacggatg ttcaggattt cccgggccag
      961 ttcaactccg gtcagttccg gcatgttatg gtcggtgacc acaagatcaa agtcctgggg
     1021 cgctgccttg aaggccataa gggcttcgtt gctgccggaa aaaaaggaga cccggtatcc
     1081 ccttttttca agcacccttt tggcaagcga cgcaagctcc tcttcgtcat cgacaaaaag
     1141 aatcctctcg caacccgaac catcaagctc ttcttcaaca acaacactct tctcaaggtc
     1201 ggggaggtcc gataccggaa aaaacaaggt aaacgttgtc cctgccccga cttcgctttc
     1261 aaccctgata attcccccgc agctttcaac tattccataa accacggaca gacccattcc
     1321 tgtgcccaca ccccgtggct tggtcgtgaa aaaaggttca aagatcgatt cctttgtctc
     1381 cgcatccata ccacagccgg tatccgatac cgtcagtcgc acgtaaaaac ccggctgccg
     1441 ctgccgatat tcctctattt catcttcagt cagttcaacc tctgccaggc ctatggtcaa
     1501 gccgcccttt tcttccatgg cctgactggc attgacacac agattgagca tgacctggtt
     1561 gatatccatg gtatcaatcc tgacaggact gcaattctgg tcaatgttca tctcgatatc
     1621 aatggatgtc gggacaacag cccgaatcat cttcatcgca tcgttcacca catcctgcag
     1681 acagagcacg gaatcgcttc tttccccctt gcggctgaac ataagtatct tcatcgtcag
     1741 ctcttttgcc ctggctgtgg cttcaagtat ctgcctgagt tcctcctcgg cgctctcccc
     1801 cgttttctgg ctgtccagcg ccatggccgc gttcagttgg atgattgcga gaatattatt
     1861 aaaatcatgg gctatgccgc cggcaagagc acctattgcc tccattttct gaacttggcg
     1921 catctgcgat tcaaaccggt gccgccttcc gctctctgtt tttgattttt tatgcattcc
     1981 ggcccataaa atggttttcc gctcatcttc aagcaaaatt atggcaatac tttttccgtt
     2041 gatatttaac gcagaaaatc cacccgtgtc aaatatatct gcaggctcaa tatgtagcct
     2101 ttccggccga cagcgttctc tttgctctgt tccgaatcat tttgttcccg tatcgtcaag
     2161 tgctctcgct tcatccttga cggagcgatg cctatcaaat tcaatcctgg ataaatcaaa
     2221 atgatactgt ttattttatg ggaagcccct ttgcgtaatg atttgacata tattttattt
     2281 tgccataata ttac
//
LOCUS       DSEB01000393            5668 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000995, whole genome shotgun sequence.
ACCESSION   DSEB01000393 DSEB01000000
VERSION     DSEB01000393.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5668)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000995"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            44..1354
                     /locus_tag="ENO07_07080"
     CDS             44..1354
                     /locus_tag="ENO07_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HDS01767.1"
                     /translation="MAGLAAQTDIPDLTDIIKSDTAVEKDTARAEGQGMVKLLTIDGA
                     IGPITVQLIEKGIRKSEQENAQALIILLNTPGGLTESTWKIIQAIMNANVPVVVYVYP
                     QGARAASAGAYITYSAHIAAMAPATSMGSASVVSMGSEMDSTMYKKVTNDAVANMVAV
                     AHKRGRNEAWIERAIREAVSISSHEAVDSNVVDLVAEDIDDLLNKIDGREVEVSMGFH
                     VLNTAGALKTEVKKTFSEKFLEIITNPNIAFLLFSLGGLGLVLELYNPGAILPGVVGG
                     ICIILAFFSFQTLPINYAGLLLILFSIVLFLLEIKVPSYGILTIGGIVSLVLGGVMLI
                     DSPAPYLRVSWYVIAAVVVVFVGFFGFAVRYVVKAHHKQVTTGAEGLVGLIGKVKQKI
                     DPTGLVLVAGELWRARADEPIEKDERVKVVSSDGMEIKVEKHSE"
     gene            1367..2134
                     /locus_tag="ENO07_07085"
     CDS             1367..2134
                     /locus_tag="ENO07_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HDS01768.1"
                     /translation="MPPSLALPITIGIIVLFILANAIRILKEYERGVIFRLGRLVGTK
                     GPGLILLIPIVDKMVKVSLRIVTMDVPPQDIITRDNVTVKVNAVIYFRVMDSNKAVVA
                     VEDYLFATSQIAQTTLRSVCGQVELDDLLSNRDEINAKLQTIIDDQTEPWGIKVSQVE
                     VKNVDLPTDMTRAIARQAEAERERRSKVIHAEGEFQAAEKLVQASERMEKSPIALQLR
                     FLGTLTEIATEKNSTIVFPIPIDMISSFMKTYIEKKE"
     gene            2264..2803
                     /locus_tag="ENO07_07090"
     CDS             2264..2803
                     /locus_tag="ENO07_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003330090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HDS01769.1"
                     /translation="MIKRDEAYFVLVDVQGKLASLMYKKYRLFKNLKILIEGLRELDI
                     PIIWAEQYPEGLGETVAEIKAILGGLEPISKKAFSVCRHPELMRKIEATNRTEAIVAG
                     IETHVCVYQTVADLLSKGHKVTIVADATSSRTKENKELGIQKMLAEGAKISSVEMILF
                     ELLGVAEGEAFKRISQLVK"
     gene            2864..4186
                     /locus_tag="ENO07_07095"
     CDS             2864..4186
                     /locus_tag="ENO07_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HDS01770.1"
                     /translation="MKTREDIIRIIDEENVRYIRLWFTDILGQLKGMSITRAELEEVL
                     DNGQGFDGSSIQGYVRIEESDLMAWPDYRTFRIIPWDIGGERVAMMFCDIKNPDDTPY
                     LGDPRNALKRMIELGAQNGYTFNFGPELEYFYFRNRNKLEFMDSGGYFDYSTIDEGTI
                     LRKKTCRHLENLGVPVECSHHEVAPSQHEIDLKYQEALVMADFCQLYRMVVRETAQKE
                     GFHATFMPKPAFGENGSGMHIHISIFNGNKNLFFSPEGEYNLSELAKSFMAGLLHHIK
                     AITLVTNQWVNSYKRLVPGYEAPCYISWGRRNRSSLIRVPMYRVGKEKSTRIELRSPD
                     PACNPYLAFTVIMAAGMDGIKNKYDLPSPIEQNIFHMTEEQKQSNKIDELPSSLENAV
                     SEFSQNELMREALGDHIFETLIANKMAEWDMYRTHVSQYELDNYLPIL"
     gene            4196..5644
                     /gene="gatA"
                     /locus_tag="ENO07_07100"
     CDS             4196..5644
                     /gene="gatA"
                     /locus_tag="ENO07_07100"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010077026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDS01771.1"
                     /translation="MEFESMSAAQIRASIIKGEFTASDIAEYFLKQAKAENSRLNCII
                     SFGDSYAARAAENISQKIAAGLAPGKLAGVPIVIKDNLITKDLRTTCASHILEDFIPP
                     YNATVIESLLKQDAIIIGKANMDEFGMGSSNEHSYFGRVPNPIDPEYVPGGSSGGSAC
                     AVASGLAPLALGSDTGGSVRQPASFCGIVGLKPSYGAVSRYGLVAFGSSFDQIGPMAR
                     TVEDCAILFSVICKHDPRDSTSANYVRPDYLEALKGRKKFSIGIPREYMAEGIDGEVL
                     KAVQDAIEKAEGQGHEITEISLPHTKQSIAAYYTIANSEASSNLSRFDGVRYGYRSTA
                     IDDLAEMYIRTRSAGFGAEVKRRIMLGTYSLSTGYYDEYYLKAAKVREIIRDDFDKAF
                     NEVDLIITPTSPTTAFKLGEKIDNPLGMYLSDIYTVSASLAGLPAISIPCGKDNKGLP
                     IGLQIIGKMFREDEILNLAYNLEREIGYNLKQ"
BASE COUNT         1481 a         1384 c         1496 g         1307 t
ORIGIN      
        1 gatcaagaaa atattactta tatgcggaat gatcgggatt ttcatggcgg gccttgcggc
       61 ccagaccgat atccccgatc tgaccgatat aatcaagtcc gacacggctg tcgagaaaga
      121 taccgctcgg gccgaggggc agggtatggt caagcttttg accatcgatg gcgctatcgg
      181 accgatcaca gtccagctca tcgagaaagg catcagaaaa tctgagcagg aaaatgccca
      241 ggcgcttata atcctcctga acacaccggg gggattgacc gaatcaacct ggaagatcat
      301 ccaggcgatc atgaatgcca atgttccggt ggtggtgtat gtttatcccc agggcgctcg
      361 ggcggcctcg gccggggcat atataacata cagcgcccat attgccgcta tggctcccgc
      421 caccagcatg ggttcggcct cggtcgtgtc tatgggttca gagatggact ctaccatgta
      481 taagaaggtc accaatgacg cggttgcaaa tatggtagcg gtagcccata agcgcggcag
      541 gaacgaggcc tggattgaac gggctatccg tgaggcggtt tccatttcca gccatgaggc
      601 ggtcgattcc aacgttgtcg acctggtggc cgaggacatt gacgacctgt tgaacaagat
      661 cgatggccgc gaggttgaag tgtcgatggg ctttcatgtg ctcaacactg ccggcgcact
      721 gaaaaccgag gtaaaaaaga cattttctga gaaattcctc gagatcatta ccaaccccaa
      781 tatcgccttc ctgcttttct cgctgggagg actggggctg gtgctcgagc tgtataatcc
      841 gggagcgata ctccccggag tggtgggcgg tatctgcata atacttgcat ttttctcatt
      901 tcaaacactg cccattaatt atgccggatt gttattaatt ttattttcaa tagtgctttt
      961 cttacttgag atcaaggttc catcctatgg catcctgacc ataggaggaa tagtctctct
     1021 tgtcctggga ggggttatgc ttatagattc acctgcaccg tacctgagag tctcgtggta
     1081 tgtaattgcg gcggttgtcg tcgtttttgt cggctttttc ggctttgcag tgagatatgt
     1141 ggtcaaggcc catcacaaac aggtgactac gggtgccgaa ggtcttgtgg gcctgatcgg
     1201 taaggttaag caaaaaatag atccaaccgg tcttgtactg gtggccggag aactatggcg
     1261 cgccagggcc gatgagccca tcgaaaagga tgagcgcgtg aaagttgtca gttctgacgg
     1321 gatggaaatc aaagtggaaa aacattcaga ataaaaagga ggatagatgc ccccgagttt
     1381 agcactaccc attacaatcg gtataatagt cctgttcata ttggctaatg ccatacgtat
     1441 tctcaaagag tatgaacgag gcgtgatctt caggctgggc cgcctggtcg gcaccaaagg
     1501 tcccggcctg atacttctga tacctattgt cgataagatg gtcaaggtct ccctgcggat
     1561 cgttaccatg gatgttccgc cgcaggatat catcacacgt gacaacgtca cggtcaaggt
     1621 caatgcggtg atctatttcc gggtcatgga ttccaacaag gctgtagtcg ctgtggaaga
     1681 ttatctgttc gcgacctccc agatcgccca gaccaccctg cgctcggtct gcggacaggt
     1741 ggagctggat gaccttttgt ccaaccgcga tgagatcaat gccaagctgc agacgatcat
     1801 cgacgaccag actgaaccgt ggggaatcaa ggtctcgcag gtcgaggtca agaatgtcga
     1861 tctgccgacc gatatgaccc gcgccattgc tcgtcaggcc gaagccgaac gtgaacgtcg
     1921 atccaaagtt atccacgccg aaggtgaatt ccaggccgcc gaaaagctgg tgcaggcctc
     1981 ggaaagaatg gaaaaatcac cgattgcttt gcagcttaga tttctcggta cgttgacaga
     2041 gatcgccacc gaaaagaatt ccactatcgt gttcccgata ccgattgata tgatctcctc
     2101 atttatgaaa acttatatag aaaagaaaga gtaacccgga tagctccata gaccccggca
     2161 gattgccggg gtctatcatt ttttggagtc agtcaattca cctggaaaca ttcacgggcc
     2221 gctcgccgtt gtagcgagta ttatcaacca caaaggagga atcatgataa aacgagatga
     2281 ggcatatttt gtactggttg atgttcaggg caagctggcc agtttgatgt acaagaaata
     2341 caggcttttc aaaaacctca agattctgat cgagggattg agggaactcg atattcccat
     2401 tatctgggcc gagcaatatc ccgaggggct gggtgagact gtggccgaaa tcaaggcgat
     2461 actgggggga ctcgagccga tatccaaaaa agccttttca gtctgccgcc atcccgaatt
     2521 gatgcggaaa atagaagcta ctaacaggac cgaagcgatt gtggccggta tcgaaaccca
     2581 tgtctgcgtt taccagaccg ttgccgatct tctttccaag gggcacaaag ttacaatagt
     2641 cgccgacgcc acctcgtcgc gtacaaaaga aaacaaggaa ctgggaatac aaaagatgct
     2701 cgccgagggc gcaaaaatca gctcggtcga aatgattcta tttgaattgc tcggggttgc
     2761 cgagggcgag gcattcaaaa gaatatcgca attagttaaa tagcggctac gcgccatttt
     2821 ttgatacagt atgaaaaact acgatatccg gaggggaaaa tagatgaaaa ccagggaaga
     2881 tattatcaga atcatcgatg aggaaaatgt ccgctacatc aggctgtggt tcaccgacat
     2941 tctgggacag ctcaagggca tgtcgatcac ccgggctgaa ctggaggaag tcctggataa
     3001 cgggcagggt tttgacggat cgtcgatcca gggctatgta cgcattgaag aatcggatct
     3061 aatggcctgg cccgactaca gaacattcag aataatcccc tgggatatcg gcggcgagag
     3121 agtggccatg atgttctgcg acataaaaaa tccggatgat acgccctatc tgggcgaccc
     3181 ccgcaatgcc cttaagcgca tgatcgaact tggcgctcag aatggttata ctttcaattt
     3241 cggcccggaa ctggaatatt tctatttccg gaacaggaat aaactggagt ttatggattc
     3301 cggcggatat ttcgattatt ccaccataga cgaggggact atcctgagaa aaaagacctg
     3361 ccgccacctc gaaaacctgg gagtgccggt agaatgttct catcatgagg tggcgccgag
     3421 ccagcatgaa atcgacctca aataccagga ggcgctggtg atggccgact tctgccagct
     3481 gtaccgcatg gtcgtcaggg aaaccgccca gaaggagggt ttccacgcta cttttatgcc
     3541 caagccggct ttcggtgaaa atggttccgg catgcatatc catatctcaa tatttaacgg
     3601 aaacaagaat ctcttctttt ctcccgaagg agaatacaac ctttccgaac tggccaaatc
     3661 gttcatggcc gggctgcttc accatatcaa ggcaatcacg ctggtgacaa atcaatgggt
     3721 caattcctat aaacgtctgg tgcccggcta tgaggccccc tgctatatct cctggggacg
     3781 gcgcaaccgc tcctccctga ttcgagtacc catgtatcgc gtgggcaaag agaagtcgac
     3841 ccgtatcgag ctgcgctcgc ccgatccggc ctgcaatccg tatctggctt tcacggtaat
     3901 aatggcggcc gggatggacg gcataaaaaa caaatatgat ctcccctcac ccatcgaaca
     3961 gaacattttc cacatgaccg aagagcaaaa gcaaagtaat aaaattgacg agctgccctc
     4021 atccctggaa aatgcggtga gcgagttcag ccagaatgag cttatgagag aggcccttgg
     4081 agatcacatc ttcgagaccc tgatcgccaa caagatggcc gagtgggaca tgtaccgcac
     4141 gcatgtatcc cagtacgaac ttgacaatta tctgcccatt ctttaggttt cctgcatgga
     4201 atttgagagt atgagcgctg cccaaatcag ggcgtccata ataaaagggg aatttactgc
     4261 cagtgacata gccgaatact tcctcaagca ggcaaaggca gaaaattccc gtctgaactg
     4321 catcatatct tttggcgata gctatgccgc ccgggctgca gagaatatta gccagaagat
     4381 cgccgcgggt cttgccccgg gcaagctggc cggcgtaccg attgtcatca aagacaatct
     4441 tatcacaaaa gatctaagga ccacctgcgc ctcgcatatc ctggaggatt ttataccccc
     4501 ttataacgcc accgtaatcg aatctctgct gaagcaggat gcgattatta tcggcaaggc
     4561 caacatggat gaattcggta tgggatcatc aaatgagcat tcatattttg gcagggtacc
     4621 gaaccccatt gaccccgaat acgtgcccgg cggatcgtcg ggcggctccg cctgtgcggt
     4681 ggcatcggga ttagcgccgc tggcactggg aagcgatacc ggaggctcgg tcagacagcc
     4741 cgcttcattc tgcggtattg tgggcctgaa gccgtcatac ggcgcggtat cccgctacgg
     4801 cctggtggcg tttgggtcat cattcgatca gatcgggccg atggcgcgga cagtggagga
     4861 ttgcgccatc ctgttttcgg tcatatgcaa gcatgatccc cgggattcta cctccgcgaa
     4921 ttacgtgcgt cccgattacc tggaagcgct caagggtcga aagaaattca gtatcggcat
     4981 accccgggag tatatggccg agggaatcga tggcgaggtt ctgaaggcgg ttcaggatgc
     5041 tatcgaaaaa gccgaggggc agggtcatga gattacagag atatcgctgc cccacacaaa
     5101 gcagtcaatc gcagcctatt acacaattgc caattccgag gcctcttcaa atctgtcgcg
     5161 ctttgacggc gtccgttacg gctatcggtc aaccgctatc gatgatctgg ccgagatgta
     5221 tatcagaacc aggagtgccg gttttggtgc ggaggttaaa cgtaggatta tgctgggcac
     5281 ctactccctc tccaccgggt actatgacga gtattatctc aaagccgcaa aagtcaggga
     5341 gataatccga gatgattttg ataaggcatt taatgaggtg gatctgatta ttactccaac
     5401 atcccccacc acggctttca aacttggaga gaagatcgac aatccccttg ggatgtatct
     5461 gtccgatatt tacaccgttt ccgcaagcct ggcgggactt cctgcaatct cgataccatg
     5521 cggtaaagac aataaggggc tgccgatagg cctgcagata atcggcaaga tgttcaggga
     5581 ggatgaaata ttgaatctgg catacaatct ggaaagagaa atcggatata atttaaaaca
     5641 atgaatgaat acgagataat catagggc
//
LOCUS       DSEB01000394            7139 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000617, whole genome shotgun sequence.
ACCESSION   DSEB01000394 DSEB01000000
VERSION     DSEB01000394.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7139)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7139)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7139
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000617"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..558
                     /locus_tag="ENO07_07105"
     CDS             <1..558
                     /locus_tag="ENO07_07105"
                     /inference="COORDINATES: protein motif:HMM:PF14450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01772.1"
                     /translation="IRHTASIGMGGKYVTNDIAVGLQIPYNLAEKIKIASGHAFSARI
                     DPVEMVEIPEIGGRQQSKVSRVLLASIIEARMEEIFELADDTVKRSGYSNKLACGVVV
                     TGGGARLRGLDKLANKVFGLPIKIGYPEKIHLPEEFYGKPEYSAAIGLLAYSLRHPVD
                     QEEKRNILVRMIHKVEDAISAIFNI"
     gene            578..1753
                     /gene="ftsZ"
                     /locus_tag="ENO07_07110"
     CDS             578..1753
                     /gene="ftsZ"
                     /locus_tag="ENO07_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018921335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDS01773.1"
                     /translation="MIEFVPDESNSARIKVVGVGGSGGNAINRMIESGLTNVDFVSIN
                     TDMQALDGSKAGTKIQIGTKITRGLGAGADPEVGRRAIEEDRDKVAEALQDADMIFVT
                     AGMGGGTGTGAAPVVAEIAKELGALTVATVTKPFDFEGRKRMNRALNGIHDLKSRVDT
                     LIVIPNQRLIAIADKDTPLMDTFLMADEVLLHATRGISDLITIPGLINCDFADVKTVM
                     KEMGDALMGTGTCSGEDRAQEAAHQAISSPLLEDVSITGAKGVLINICGGSDITLHEV
                     NAATSIISEAAGSDANIIFGAVIDPDIKGEFRVTVIATGFGKPRPEAEFDTRVVDLFS
                     QQDNARRPKSRIEREIIEEEPREGQQEVGWQQPVTVDNAEVPTFIRKNYQKDFDSYK"
     gene            1906..2355
                     /locus_tag="ENO07_07115"
     CDS             1906..2355
                     /locus_tag="ENO07_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013652547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HDS01774.1"
                     /translation="MQKFEPQNQEFEITIRQSFKKQNLMQALGARLVAIALGEVTIEL
                     PYREDLTQQHGYLHAGVITAVIDSACGYAALSLMPAGMEVLSVEYKINLLSPADGERL
                     TAKGVVLKPGRKLYVVRGDAYSIKDGGEKHVAAFQGTMVAARKRDKI"
     regulatory      2428..2520
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2567..3382
                     /locus_tag="ENO07_07120"
     CDS             2567..3382
                     /locus_tag="ENO07_07120"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDS01775.1"
                     /translation="MIDPGKDAGENLRKLREQKPLIHNITNYVVMNFSANSLIAMGAL
                     PVMAHAENEVEEMASIAGALVINIGTLSDPWIRAMLLAGKTANKNNIPIILDPVGAGA
                     TSLRTNTAKRLAKELDIAVIRGNASEVLSLSGEESGTKGVDSIHSVEDAAQAAVELAL
                     ELDSVIAITGENDLVTNGDTVFRVHNGHKLMGMVTGSGCAATAAIAAFLAVNDNFVEA
                     AAFALGYFGLAGQWSAIRNTRPGSFKVGLIDSLYEIGPDDLQTGLKLEVMHET"
     gene            3372..4013
                     /locus_tag="ENO07_07125"
     CDS             3372..4013
                     /locus_tag="ENO07_07125"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDS01776.1"
                     /translation="MKPDYSLYLVTDRRLSKGRTNIEIIEAAIRGGVTAVQLREKHAT
                     TREFLHEALKVRDYCREKNVLFIVNDRLDIAQSVDADGVHLGQDDLPIEYARRFLGPD
                     KIIGASAFNESEAVEAEKAGADYLGISPIFTTPTKPELETGIGIGGLRKIRKSVEIPM
                     VGIGGLGSTNAYDVITAGADGIALVSAIVSAADPERAAREIKSEIERARKDML"
     gene            3977..5014
                     /gene="thiL"
                     /locus_tag="ENO07_07130"
     CDS             3977..5014
                     /gene="thiL"
                     /locus_tag="ENO07_07130"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HDS01777.1"
                     /translation="MRDRKGQKGHAVKLKDIGEFGFIDKIKHDALIREYLGLVGIGDD
                     CAVFRVEEGKSILLTTDMLLERVHFLREVMTPYQIGYKALAANLSDIAASGGLPKEAF
                     VSIAVPEDLSLEELLELYDGMKELARKFDVNITGGDTTTSLQDLVINIALTGEIEEDL
                     ALYRSGAKAGDYICVTGFLGDSAAGLDLILNHRKEMHNFPQLIKQHFTPIPHIEQGRA
                     VSRSRLANSMMDISDGLASDIRHISKASKLGAILYEERFPHSEEFQKYLAEFNPDYLK
                     TAIGVGEDYCLLLTVSQQNYARLKEVLNNLGHQFYNVGEMAEGEEIHIVYKNGRKAIL
                     ELSGWDQFKEH"
     gene            5017..5829
                     /gene="thiD"
                     /locus_tag="ENO07_07135"
     CDS             5017..5829
                     /gene="thiD"
                     /locus_tag="ENO07_07135"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDS01778.1"
                     /translation="MRNILPVVLTIAGSDSGGGAGIQADLKTFSSLGTFGTTVITAIT
                     SQNLEGVRSIQAVKADIVRDQIESVFDGFKVNAVKTGMLFSSEIIETVAETIRKYDVE
                     NLVIDPVFAATSGSRLIEDDGIEALTEELFPMAKVITPNIPEAEFLTGMEIKNKQQMT
                     EALEALIELYPASVFVLKGGHLDDAAIDVYGGKSLAVNELKSDKVTGVNNHGSGCTFA
                     SAVAAYLAHGHEIEESLNLAKNFISGALRNSLGLEQGINLIDHFWRSEKGIH"
     gene            complement(5830..6255)
                     /locus_tag="ENO07_07140"
     CDS             complement(5830..6255)
                     /locus_tag="ENO07_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01779.1"
                     /translation="MAKIKEQFRETIQWSKGVQKRLTGTRSPRHKELDVHQILESLFK
                     QVDRNKDEYVKKIDVCKRKYISDSETIKMALIKLLTSHEHEQEISPEKAGRLQELLQE
                     RFEDEDSQRDEIGVLKEKENSLAALEKEVIGILSRRFKK"
     gene            complement(6242..>7139)
                     /locus_tag="ENO07_07145"
     CDS             complement(6242..>7139)
                     /locus_tag="ENO07_07145"
                     /inference="COORDINATES: protein
                     motif:HMM:PF04101.14,HMM:PF06925.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01780.1"
                     /translation="SWFFRKLIMGKFIRRIKKVRPDIIISTHFMPSDAVYLLNRKMNR
                     PAKFAIIVTDYGIHTAWLTPGADRFYVPTEAIKAELLTLKEFLCIESDNVKVTGIPIH
                     PKYTNIEDKQTLRSKYNLDPDIFTVLLFRDVFTKGNFEHFVRYLTMVPHPIQLIMLAG
                     KEWPIPDRIKRRFAENNVSYRIFGYIDFMEELMALADIVITKSGGLTTSECMAAGTPI
                     AIYKPYAGQEERNAEFLMEQGAGIKLNQLAGLPYRLTELIENPEKLKDLSAGARMIAK
                     PNAAYDIVKDLKKIGGDSSGKN"
BASE COUNT         1816 a         1750 c         1883 g         1690 t
ORIGIN      
        1 atccgtcaca ccgcctcaat cggtatgggg ggcaaatacg tcaccaacga tatcgctgtg
       61 ggacttcaga tcccctataa tctggcggaa aaaataaaaa tcgccagcgg tcatgcattt
      121 tccgccagga tcgatccggt tgagatggtt gaaattcccg agatcggcgg ccgccagcaa
      181 tccaaagtct cccgcgtgct tctggcttcc attatagaag cccgcatgga ggaaatcttc
      241 gaactggcag acgacaccgt caagcgctcc ggttattcca acaagctcgc ctgcggcgtg
      301 gtggtgaccg gcggaggggc gaggctgcgc ggattggata agttggctaa caaggtattc
      361 gggctgccaa ttaaaatcgg ttaccccgaa aaaatacatc tgcccgagga attctacggt
      421 aaaccggaat actcggctgc gatcgggctt ctggcctaca gcttgaggca tccagtcgat
      481 caggaagaaa agagaaatat cctggtccgc atgatccata aggttgaaga cgcaataagt
      541 gccattttca atatataaga caagaggagg aagagcgatg atagagtttg ttccagatga
      601 aagcaatagc gccagaataa aggtcgttgg ggtaggcgga tcgggaggca acgccatcaa
      661 ccgcatgatc gaatccggtc tgaccaacgt tgattttgtc tccatcaata ccgacatgca
      721 ggctctggac ggcagcaagg ccgggaccaa aattcagatc ggtaccaaaa taaccagggg
      781 ccttggtgca ggggccgatc ccgaggtcgg acggagagca atcgaagagg atcgcgataa
      841 ggtggcggaa gcgctccagg acgcagatat gatttttgtg accgcaggaa tgggcggggg
      901 caccggaacc ggcgctgccc cggtagtggc cgagatcgcc aaagagctgg gcgcactgac
      961 tgtggccact gtcaccaagc cgttcgattt cgagggccgc aagaggatga accgcgcctt
     1021 aaacggaatt cacgatttga aatcccgcgt ggacacattg attgtcatcc ctaaccagcg
     1081 gctgatagct attgccgata aggatacccc gctgatggat acttttctca tggccgacga
     1141 ggtgctgctg catgctaccc gcggcatttc cgacctgatc accataccgg ggctgattaa
     1201 ctgtgacttc gccgatgtca agaccgtcat gaaggagatg ggggatgccc tgatgggaac
     1261 tggcacatgc tccggggagg accgcgccca ggaggcggcc caccaggcaa tcagttcgcc
     1321 gctgctggag gatgtctcca ttaccggcgc caagggtgtg ctgatcaata tctgcggcgg
     1381 ctcggatata actctgcatg aggtcaatgc cgctacatcg atcatttccg aggcggccgg
     1441 ctccgatgcc aatatcatct tcggcgccgt gatcgacccc gatatcaagg gcgaattcag
     1501 ggtgactgtg attgccaccg gattcggaaa gcccaggccg gaagccgaat tcgatacccg
     1561 ggtggtcgac ctgttcagtc aacaggacaa tgcccgccgg ccgaaatcaa ggatcgaaag
     1621 ggaaattatc gaggaggagc caagagaggg gcagcaggaa gtcggttggc agcagccggt
     1681 aacagtggac aacgctgagg tgccgacctt cattcgcaaa aattaccaga aggactttga
     1741 ttcttataaa tagttgaaag cattacacct ctagtcgctg atgtcttaaa tccccggagg
     1801 ggggagggag gccctgtctg agagatcagg cagggcttat tttattcttc ttgttgaccg
     1861 cgcgatcata aatgcattat aattattgta atagatggac ttattatgca aaaattcgag
     1921 ccccaaaatc aggaattcga aattacaatc cgccagagtt ttaagaaaca aaacctgatg
     1981 caggctcttg gagccagact ggtcgctatt gccctggggg aagtcaccat cgagttgcca
     2041 taccgcgagg atttgaccca gcagcatgga tatctgcatg ccggggttat tacagcggtg
     2101 atcgattcgg cttgcgggta tgcagctctg agcctgatgc cggcaggtat ggaggtcttg
     2161 agcgttgagt ataaaatcaa cctgctctcg cccgcagacg gagagaggct tactgccaaa
     2221 ggggttgtgc tcaagccggg cagaaaattg tatgttgtca ggggagatgc atattctatc
     2281 aaagacggcg gggagaaaca tgtggcggcc tttcagggca ctatggttgc ggccaggaaa
     2341 agagataaga tttaatctcg cttcaatatt atccattttc gtcttgacat cagcagccat
     2401 taatcgcaga ttgtgggtta agttgaaata tcttctatgg ggtggccgaa aggtctgaga
     2461 tcaaacccat tgaacctgat acgggtaatt ccgacgtagg gatttgagtt ttttggccgc
     2521 ttgcctgaat ccggggcggc tttttttgtt aatgacagga ggaaaaatga ttgatccggg
     2581 aaaagatgcg ggagagaatc tgagaaaact gcgcgagcaa aaacccctta tacacaatat
     2641 aaccaattat gtggtaatga attttagcgc caattcgctt atcgccatgg gcgccttgcc
     2701 ggttatggcg catgcggaaa atgaggtgga ggagatggct tcgattgcgg gcgccctggt
     2761 aattaacatc ggcaccctct cagatccctg gattcgggcc atgctcctgg cgggaaaaac
     2821 tgccaacaaa aacaacatcc cgatcatcct cgatcccgtg ggggccgggg ctaccagcct
     2881 tcgcaccaac acagccaaac ggctggctaa agagctggat atcgccgtca taaggggcaa
     2941 cgcttccgaa gttctttctc tttcgggaga agaaagcgga accaaagggg tcgattcgat
     3001 tcattcggtc gaggatgccg cccaggccgc tgtcgaactt gcgctggagc tggattctgt
     3061 aatagccata accggggaga atgatcttgt gaccaacgga gacaccgtct tcagggtaca
     3121 taacggtcat aaattgatgg gcatggtgac cggcagcgga tgcgcagcca cagcagctat
     3181 cgccgctttc ctggctgtaa atgacaattt tgtggaggcc gccgccttcg ccctgggtta
     3241 ctttggtctc gccgggcagt ggtcggcgat aagaaacacc cggcccggat cattcaaggt
     3301 cggattgata gattccctct atgaaatcgg gcccgatgat ctccagaccg gcctgaagct
     3361 ggaggtaatg catgaaacct gattacagcc tctacctcgt taccgatcgc agactttcaa
     3421 agggccgtac taatatagag attatagagg cggccataag ggggggcgtg acagcggttc
     3481 agcttcgcga aaagcatgcg accacccgcg agttcttgca tgaagcgctc aaggtgcgcg
     3541 attactgccg cgaaaagaat gtactcttca tcgtaaatga caggctcgat atcgcccagt
     3601 cggtcgacgc tgatggtgtg cacctcggcc aggatgatct gccgatagaa tatgcccgca
     3661 gatttctcgg ccccgataaa ataatcggag cctcagcttt taatgaatca gaagcggtcg
     3721 aagcagaaaa ggcaggtgcc gactacctgg gaatttcccc gatcttcacc acccccacca
     3781 aacccgagct ggaaacagga atcggtatcg gtggattgcg taaaatacgc aaatcggttg
     3841 aaattccgat ggtcggcatc ggcgggctgg gttcgaccaa tgcttatgac gtcattacgg
     3901 ctggagcaga tggcatcgct cttgtctctg caattgtctc tgctgccgac ccggaacggg
     3961 cggcgagaga gataaaatcc gagatagaaa gggccagaaa ggacatgctg tgaaactgaa
     4021 agatataggc gaattcggat tcatagataa aattaagcat gacgccctga ttcgtgagta
     4081 tctcggtctg gtgggtattg gtgatgattg cgcggtcttc agggtcgaag agggcaaatc
     4141 gatcctgctc actaccgaca tgcttttgga gagagtacat ttccttcgag aggttatgac
     4201 tccctaccag atcggttata aagccctggc cgccaatctc tccgacatcg ccgcctcagg
     4261 cggtctgccg aaagaggcct tcgtatccat cgctgtcccg gaggatctgt cgctcgagga
     4321 gttgttggag ctgtacgacg gcatgaagga gctggcacgt aagttcgatg tcaatataac
     4381 cggcggagat acaacaactt cactccagga cctggtcata aatatcgccc tcacaggcga
     4441 aatcgaggag gatctggccc tgtatcgatc aggcgccaag gctggtgact atatttgtgt
     4501 caccggtttt ctcggtgact ctgcggccgg gctggacctg atcctgaacc atagaaagga
     4561 gatgcataat tttccgcaac tgattaaaca gcactttacc cccatcccgc acatcgagca
     4621 gggtcgagcg gtctcccgct ccaggctggc aaactccatg atggatattt ctgatgggct
     4681 ggcatccgac atccgccaca ttagcaaggc ttcgaaactg ggtgcgatac tttacgagga
     4741 aagatttcct cattcagaag agtttcaaaa atatcttgca gaattcaacc ctgattatct
     4801 caaaacagct atcggggtgg gtgaggatta ctgtcttctt ctgacagttt cgcaacagaa
     4861 ttatgccagg ctaaaagagg ttctgaataa cctcggtcat caattttata atgtgggcga
     4921 aatggctgaa ggcgaagaga ttcacatcgt ttacaaaaac ggccggaagg cgatcctcga
     4981 actcagcggg tgggaccagt tcaaggagca ttgataatga gaaatatatt acctgtggtt
     5041 ttaaccatag ccggctcgga ctccggcggc ggagccggta ttcaggctga cctgaaaaca
     5101 ttttcatcgc tgggcacatt cggcacgacc gtcataacgg cgataacttc acaaaatctg
     5161 gaaggtgtac gctcaatcca agccgtcaaa gctgacatag tcagggacca gatcgaatct
     5221 gtctttgatg gcttcaaagt aaacgctgtc aagaccggca tgcttttttc gagtgagatt
     5281 atcgagaccg tggctgagac cataagaaag tatgatgtgg aaaaccttgt catagacccg
     5341 gtattcgccg caacctcagg ttcccggctg atcgaggatg acggcatcga agctctgacc
     5401 gaagagcttt ttcctatggc taaagtaatc acccccaata ttccggaggc ggaatttctg
     5461 accggtatgg agataaaaaa taaacagcaa atgaccgagg cattagaagc tctgattgag
     5521 ctctatccgg catcagtatt tgtattaaaa ggaggccatc tggatgatgc agcaatcgat
     5581 gtctatggcg gcaagtcatt ggctgttaat gaattgaaat ccgacaaagt gaccggcgtt
     5641 aacaatcacg gctccggatg cacattcgca tcggcggtgg ccgcatattt agcgcacggt
     5701 catgaaattg aggagtcgct taatcttgct aagaacttta tcagtggggc cttacgaaac
     5761 agtctcggac tggagcaggg aatcaatctg atcgaccatt tctggcgctc ggaaaagggg
     5821 atacactaat cacttcttga atcgacggga cagaatcccg atcacttctt tttccagggc
     5881 ggcgagagaa ttttccttct ctttcagcac accaatttca tctctctggc tatcctcgtc
     5941 ttcaaaccgc tcctgcagga gctcctgcag tcggccggct ttctccggcg agatttcctg
     6001 ctcatgttca tgagaggtca aaagcttaat cagggccatt ttaattgtct ccgagtcgga
     6061 gatgtatttc cgcttgcaga catcgatctt cttgacatac tcatccttgt tacggtccac
     6121 ctgcttgaaa agcgattcca ggatctggtg aacgtcaagc tccttatgcc tggggctcct
     6181 cgtccccgta agcctcttct gaacaccctt cgaccactga atggtctccc tgaattgttc
     6241 cttaattttt gccactgcta tcccctccta tctttttcaa atcttttaca atatcgtaag
     6301 ccgcattggg cttggcgatc attctggcac ctgccgacag atctttcagt ttctccggat
     6361 tctcgattaa ttcagttaac ctgtacggca atccggccag ctggttcagt ttaattcccg
     6421 caccctgttc catcaggaat tcagcgttac gctcttcctg accggcatag ggcttgtata
     6481 tggcaattgg tgtccctgca gccatgcatt cggaggtcgt caaaccgccg gacttggtta
     6541 ttactatatc ggccaatgcc atcagctcct ccataaagtc aatataaccg aaaatcctgt
     6601 atgatacatt gttttctgca aatcttctct tgatcctgtc aggaatcggc cactccttgc
     6661 cggccagcat gatcagctgg attggatgag gcaccatggt cagatacctg acgaaatgtt
     6721 cgaaattgcc ttttgtgaat acatcccgaa acaaaagaac tgtgaaaata tcgggatcaa
     6781 ggttgtattt agatctcaat gtttgcttgt cttcaatgtt agtatacttg ggatgaatcg
     6841 gaattccggt aactttcaca ttatcacttt caatacatag aaactctttc aacgtcaaaa
     6901 gttccgcttt gattgcctcg gtgggcacat aaaatctatc tgcgcccggt gtcagccagg
     6961 ctgtatggat gccgtaatcg gtcacaatta tggcgaattt ggccggacga ttcatcttcc
     7021 tgttcaacaa ataaaccgca tcagagggca tgaaatgagt tgagatgatt atgtcaggtc
     7081 tgaccttctt tatacgccgt atgaacttgc ccattatcag cttcctgaaa aaccagctc
//
LOCUS       DSEB01000395            4919 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001376, whole genome shotgun sequence.
ACCESSION   DSEB01000395 DSEB01000000
VERSION     DSEB01000395.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001376"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..303
                     /locus_tag="ENO07_07150"
     CDS             <1..303
                     /locus_tag="ENO07_07150"
                     /inference="COORDINATES: protein motif:HMM:PF14870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01781.1"
                     /translation="EFNSGWLVGDSGTYYTSQDGGWSWDDRGGQFPNVNLYDIVFIDR
                     FSGFIVGEGGMIMMSVDGGNTWQFDDNMTTNTLHDITFDGSKLWICGDSGTIIFVH"
     gene            488..781
                     /locus_tag="ENO07_07155"
     CDS             488..781
                     /locus_tag="ENO07_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01782.1"
                     /translation="MGYAGGAAGGAAAAAAAIANAIKSSGAIVSLEPEEFQRILSRAD
                     APLIVHTRAGWPKKTDRYLTSYKGLFFYTKSDKPLMLPSGAELVESNKIMIPG"
     gene            complement(840..1601)
                     /locus_tag="ENO07_07160"
     CDS             complement(840..1601)
                     /locus_tag="ENO07_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008880475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDS01783.1"
                     /translation="MNRSQIVKYLHRIGIEKAQSPDLRFLAALQKQHLLNVPFENLDI
                     RRGREIVLDCDSLYKKIVLERRGGYCYELNGLFCKLLRELGYSVDMLSARVFNSAGIL
                     SQEFDHMTLMVHLDNDYLVDVGFGDSSRKPIEMPGGETEDVSGKYRIKSNPEIESCFF
                     LQKFTDQDWHPRYRFTTIPRELADFQGMNEYHQTSPESHFTQKSICTIATEEGRISLS
                     DKFLTITEGTEKDKSPLPSGDHFKNYLKQYFDIEL"
     gene            complement(1702..3006)
                     /locus_tag="ENO07_07165"
     CDS             complement(1702..3006)
                     /locus_tag="ENO07_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HDS01784.1"
                     /translation="MADLSDKLKNKKAVVGIIGLGYVGLPLAVEFAQNGLKVIGFDIA
                     KTKVDLINSGQSDIRDVASETVKILIDSGMLSATDDFSRLKSVDCTAICVPTPLSKTK
                     DPDVAFILSAMDQIGKYVHKEMLIVLESTTYPGTTEELILPGLEQKGLKVGQDFYLAF
                     SPERIDPGNKTYHTANTPRVVGGVTPECREIAKQFYRCIVDDVIDVSSTKAAEMVKLL
                     ENTFRSVNIGLVNEVALMCDRLGLDVWEIIDAASSKPFGFMPFYPGPGLGGHCIPIDP
                     HYLSWKLKSLNYYARFIELAGDINTHMPEYVIDRVTRHMNDYGQALKNARIVVLGVAY
                     KPDISDVRESPALDVIKLLQDRGAKVMYNDPYVPIVKFDSGDMKSQKLTKKLLESAAC
                     TIIVTNHSDYDYQWVVDHSNLVFDTRNATKNVKGSKRKIAKL"
     gene            complement(3016..3966)
                     /locus_tag="ENO07_07170"
     CDS             complement(3016..3966)
                     /locus_tag="ENO07_07170"
                     /inference="COORDINATES: protein motif:HMM:PF16363.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDS01785.1"
                     /translation="MKALITGVPGFAGSHLAENLLGHNYEVYGTHLPGESLRNLHGFR
                     GELELSAMDFHKPERIPGLLKKIEPDYIFHLAALPSVAGSFLDPLETYTVNFMGSFHL
                     MQAALDLKQLKKLIIISSSDIYGLVKQKDLPIKIEHPLQPVSPYAVSKATCDMMGYQY
                     FINYGLPVVRIRAFSHSGPRQARGFVIPDFSFQIAQLEKFRKRRRVIRVGNLNARRDI
                     SDVRDIANGYRLAAEKGKPGEVYQLCSGKAYAISTILKKLIKLSHHKIEIETDPKLSR
                     PTDIPVLKGDASKAEKSLGYKRKYSIDDTLRDCLDYYRSL"
     gene            complement(3966..>4919)
                     /gene="gmd"
                     /locus_tag="ENO07_07175"
     CDS             complement(3966..>4919)
                     /gene="gmd"
                     /locus_tag="ENO07_07175"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HDS01786.1"
                     /translation="GITGQDGSYLAEFLLEKGYDVYGMVRRSSTENFERINHIKDQLN
                     FVQGDLLDYNSLSSIIKTVNPDEVYNLAAQSFVPTSWTQPSLTAEFNAVGVTRLLEAI
                     RDHNVKIKFYQASSSEMFGKVREVPQTEKTPFYPRSPYGIAKVYAHWITVNYRESYNI
                     FACSGILFNHESPRRGLEFVTKKITDAVARIKLDLATELRLGNLEAKRDWGYAGDYVR
                     AMWLMLQQDEPDNYVIATGENHSVKEFVDIAFGHVDLDPGKYVKVDERFLRPAEVDHL
                     LGDSSYALEKLGWKPEVSFEEMVKMMVDYDLEKYRKIKGID"
BASE COUNT         1203 a         1165 c         1185 g         1366 t
ORIGIN      
        1 gaatttaatt ccggatggct ggtgggcgac agcggtacct attatacctc gcaggacggc
       61 gggtggtcat gggatgaccg cgggggacag tttccgaacg tgaatctgta tgatattgtc
      121 tttatagacc gcttcagcgg atttatagtg ggtgagggcg ggatgataat gatgagcgta
      181 gacggcggca atacctggca gtttgacgac aacatgacca ccaacactct tcatgacatc
      241 acctttgatg gtagcaaact ctggatatgc ggcgacagcg gcaccatcat ttttgtgcat
      301 tagctctttt gctgtactct gctttgtaaa ttttgaggga aagtctcaac agctgcgctt
      361 cagcaactct gcttttgaca aagcggagtg cccaatgcaa ccaattcatt ttaatctttt
      421 ttcttgaata aaatcctctg gatagccata tatgtactat aatatattgt tccgaaagga
      481 gagctaaatg ggttatgctg gaggtgcggc aggcggtgcg gccgcggccg ctgcagcaat
      541 tgccaatgcg ataaaatctt ccggggctat agtcagtctg gagccggagg aatttcagag
      601 gatactctct cgtgcggatg ctcccttgat tgtgcatacg cgtgccggat ggccgaaaaa
      661 aacagaccgg tatttgacgt cgtataaggg tctgttcttt tacaccaaat ccgacaagcc
      721 gcttatgctg cccagcggcg ccgaactggt ggaatcaaat aagatcatga ttccgggcta
      781 agataaaaaa ggcagggcca gaatttagcg tgcctgcccg ggtctaattt tgaacaaaac
      841 tataattcta tatcaaaata ttgcttcaaa tagttcttga aatggtcccc ggacggcagc
      901 gggcttttat ccttctcagt gccttctgta atggtcagga atttatcgga taggctgatg
      961 cggccctctt ctgttgcgat tgtacaaatc gatttttgtg tgaaatgcga ttctggtgaa
     1021 gtttgatggt attcattcat gccttgaaaa tcagctaact cgcggggaat cgtggtaaac
     1081 cgataccgcg gatgccagtc ttgatcagta aatttttgca gaaagaagca ggactcgatt
     1141 tccgggtttg atttgattct gtacttcccg cttacatctt ctgtttcgcc acccggcatc
     1201 tcaatcggtt tcctggatga atcgccaaag ccgacgtcta caagatagtc gttgtcgaga
     1261 tgcaccatca gtgtcatatg atcgaattcc tggctcagga tgcccgcgga gttgaataca
     1321 cgtgcagaga gcatgtcaac agagtagccc agttcccgca ataatttgca gaaaagacca
     1381 ttgagttcat agcagtaacc cccgcgcctc tcgagaacta tctttttgta tagactatcg
     1441 cagtcgagca caatctcgcg tcctcttctg atatcaagat tctcaaacgg tacatttaga
     1501 agatgctgtt tttgcagagc ggcgagaaat cgcaggtctg gagattgcgc tttctcgatt
     1561 ccgattctat gcagatattt tacaatttgc gacctgttca tcatgattta tttagttatt
     1621 atgaaatcaa ggtctcgctt gacattaata acgagcctgc ctgatttagt caggcaggca
     1681 tcaggcttaa tctaagttta actacaattt agctatcttg cgttttgaac ccttgacatt
     1741 cttggtggcg tttctggtat cgaagaccag attggaatga tctaccaccc actggtaatc
     1801 gtagtccgaa tggttcgtga ctataatcgt acatgctgct gattccagaa gttttttcgt
     1861 gagcttctgt gatttcatgt cgccggaatc gaacttgact atgggaacat agggatcatt
     1921 atacatgacc ttcgcaccgc ggtcctgcag gagtttgatc acgtcgagtg caggggattc
     1981 gcgcacatca gaaatgtcgg gtttataggc aactcccaga acgacgatcc tggcgttctt
     2041 gagagcctgg ccataatcat tcatatggcg cgtcacccga tcaatcacat actcgggcat
     2101 atgtgtattt atgtctccgg ccagttcgat gaaacgggca taatagttca gcgatttgag
     2161 tttccacgag aggtaatgcg gatcgatggg gatgcaatgg ccgcccagtc ccggcccggg
     2221 atagaatggc atgaacccaa aaggtttgga ggatgcggca tcgattattt cccatacatc
     2281 cagtccgaga cggtcgcaca taagggcgac ttcattcacc aggccgatat tgaccgaacg
     2341 gaaggtgttt tccagaagct tgaccatttc cgcagccttg gtggaggaaa catcaatgac
     2401 atcatcgact atgcacctgt agaattgttt cgctatttca cggcattcgg gagtgactcc
     2461 tcccacaacc ctgggcgtat tggcggtatg gtaggtcttg tttccgggat caattcgctc
     2521 gggcgagaaa gccagataga aatcctggcc gacttttagt cctttttgtt ccaatcccgg
     2581 caatatcagt tcttcggttg tacctgggta agtcgtcgat tccaggacaa tcaacatctc
     2641 tttatgcaca tatttcccga tctggtccat tgcgctcagt atgaaggcta catccggatc
     2701 cttggtcttg gagaggggcg tgggaacaca gattgcagtg cagtcaaccg atttgagccg
     2761 ggagaaatca tcagtggcgg aaagcatgcc ggaatctatc agtatcttga cagtttcaga
     2821 ggcgacatct cttatatccg attgccccga atttattaga tcaactttcg tctttgctat
     2881 atcaaatcca atcaccttga gaccattctg ggcgaattcg acggccagcg gaagacctac
     2941 atatccgagc cctattattc caaccacagc ctttttgttt ttcagtttgt cgctaagatc
     3001 ggccatttct cctccttaga gcgatctata ataatccaaa caatctctta atgtatcgtc
     3061 aatggaatac tttcttttat atcccagact tttttctgcc ttcgaggcat cccccttgag
     3121 tacgggaata tcggtaggac gcgagagttt tggatcggtt tcaatttcta ttttatggtg
     3181 tgaaagttta atcaattttt tcaatatggt tgatattgcg tatgcttttc cgctgcagag
     3241 ctggtaaact tcccccggtt tccccttttc ggccgccaga cgatagccgt ttgctatatc
     3301 ccggacatcc gatatatctc tgcgtgcatt aaggttcccg accctgatta cccgtcttct
     3361 cttcctgaat ttctcgagct gggcgatctg gaaactaaaa tcagggatga caaatccccg
     3421 ggcctgacgg ggcccggaat gcgaaaaggc acgtattcta acaactggca gcccgtagtt
     3481 tatgaaatac tggtaaccca tcatatcgca ggtcgccttt gatacggcat acggcgaaac
     3541 aggctgtaat ggatgctcga tctttatagg aaggtccttc tgtttcacca atccataaat
     3601 gtccgaagat gatattatga tcagtttctt gagctgcttc aaatcgagtg ccgcctgcat
     3661 gagatgaaag ctgcccatga aattcacagt gtatgtttcc agtggatcta gaaatgagcc
     3721 tgccactgat ggcaaagcgg cgaggtgaaa gatgtagtcg ggctcaatct ttttcagaag
     3781 tcccggaatc ctttcgggct tatggaagtc catggccgaa agttcaagct cgccgcggaa
     3841 tccgtgtaga ttcctgaggc tttcccccgg cagatgagta ccgtaaacct cgtaattatg
     3901 ccccagcaga ttttcggcca ggtgtgagcc tgcaaatccg ggaacccctg tgatcagagc
     3961 tttcattaat cgatgccttt tatcttcctg tatttttcca ggtcataatc gaccatcatt
     4021 ttgaccattt cctcaaacga cacctcgggc ttccatccca gcttttcaag ggcatagctg
     4081 gagtcgccca ggagatgatc cacctcggcc ggcctcaaga agcgttcgtc aaccttaacg
     4141 tatttcccgg gatcgagatc gacatggcca aaagctatat cgacaaattc tttgactgag
     4201 tggttttcgc cggttgcgat aacataatta tccggctcat cctgctgcag catcagccac
     4261 atggcgcgca cataatcgcc ggcataaccc cagtcacgtt tggcttctaa attccccagt
     4321 ctgagttctg tggccagatc cagctttatc ctggcgacag catcggtaat cttcttggtg
     4381 acaaactcaa gcccgcgacg gggagattcg tggttgaaca gaatgccgga gcaggcgaat
     4441 atattatagg attcccggta attgaccgta atccaatgtg cgtagacctt ggcgatacca
     4501 tagggggaac gggggtaaaa tggagttttt tcggtctggg gaacctcgcg caccttgccg
     4561 aacatctccg aggaagaggc ctggtagaat tttattttta cattatggtc cctgattgct
     4621 tccagcagac gggttactcc aaccgcatta aattcagctg tcaggctggg ctgggtccat
     4681 gatgtgggca caaatgactg tgccgcaaga ttatagacct catccggatt aaccgtcttg
     4741 attatggatg agagggaatt gtagtccagg agatccccct gcacaaaatt gagctgatcc
     4801 ttaatgtggt ttatccgctc gaaattttcg gttgatgaac gccgcaccat gccgtaaaca
     4861 tcatagcctt tctccagcag gaattctgcc agataggaac cgtcctggcc ggttatacc
//
LOCUS       DSEB01000396            5066 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001274, whole genome shotgun sequence.
ACCESSION   DSEB01000396 DSEB01000000
VERSION     DSEB01000396.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5066)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001274"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            262..543
                     /locus_tag="ENO07_07180"
     CDS             262..543
                     /locus_tag="ENO07_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase F0F1"
                     /protein_id="HDS01787.1"
                     /translation="MSGKFGEKISKKEKRKLKARREKDKSGLWFWLGMFGMVGWSVAI
                     PTIVCLAIGIWIDSTHPGQISWTLTFLVIGVVLGSLNAWYWIKRESRHD"
     gene            503..1771
                     /locus_tag="ENO07_07185"
     CDS             503..1771
                     /locus_tag="ENO07_07185"
                     /inference="COORDINATES: protein motif:HMM:PF00999.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDS01788.1"
                     /translation="MPGTGSNGRAGMTESSDIILLTFVVGLILLAVRLFGSYLKRLGL
                     PALVAFILIGVILRASHYQWHWITSECEDVFEFLAKLGLVALLFRIGLESNIKMLFER
                     LKSASFIWIGNIVISFLAGYWTAYYLLDLNLVPSLFIGSAMVATSVGISVGIWKEAEQ
                     LKSRRGEILTDVAELDDITGIIVIAVLFSIVPFLKQGSFSGALFSEVLRETGLVLLKL
                     VLFGAICVLFSIYLEQRVVSYFKNLQKKSEPIIVIAGLGIVLASLAGLLGFSLAIGAF
                     FAGAAFSRDPEHVKIDAAFSPLYSFLSPFFFIGIGLKVALASLSGAIIVGLILTVAAI
                     FGKIIGTCTPAVFGEGWKGASLLGVSMIPRAEIALIIMQKGLALGAWAVSSKLFSAMV
                     FVSAATCILTPFAVRAMLHKWPQAQTGGAG"
     gene            1738..2079
                     /locus_tag="ENO07_07190"
     CDS             1738..2079
                     /locus_tag="ENO07_07190"
                     /inference="COORDINATES: protein motif:HMM:PF12966.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01789.1"
                     /translation="MAPGTNRRCGLIHISLQIIIGFAIGLVLGAFNYSLLWMTVNRLM
                     KSGSPGLITLFSFFVRMAVILLVFYILAVRAGWISVAAGLAGLILIRAVMVGKFGPGQ
                     QSYRTKGDRRV"
     gene            2076..2804
                     /locus_tag="ENO07_07195"
     CDS             2076..2804
                     /locus_tag="ENO07_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010476591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDS01790.1"
                     /translation="MTISPDSIIYWEWKFITLNATIVFTWVVMAILVLISILVTRNLK
                     SGFKMSRWQSFLETVVEYIRDQIRQIVREDPKRYLPFLGTLFMFISLSNLLAFVPEYH
                     PPTGSIYTTGALAICVFFAVPIFGISRRGVGGYLKHYLKPTPIMFPFLVISEFSRTLA
                     LAVRLFGNVLSGTMIAAVLLAVAPLFVPVVMQVLSLLIGQIHAYIFAILAAVYIGSAS
                     RARSEEEHQNDENNQTENKDEEDN"
     gene            2804..3085
                     /locus_tag="ENO07_07200"
     CDS             2804..3085
                     /locus_tag="ENO07_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDS01791.1"
                     /translation="MDSISIIGAASVLGAGLAIAIGSIGPALGEGRALARALDSIAQQ
                     PDESNTITRTLFVGMAMIESTAIYCFVIAIILIFANPFWNYVTAQAGGQ"
     gene            3087..3944
                     /gene="atpF"
                     /locus_tag="ENO07_07205"
     CDS             3087..3944
                     /gene="atpF"
                     /locus_tag="ENO07_07205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HDS01792.1"
                     /translation="MQIDWFTLIAQIVNFLILVWLLKHFLYDRIVDSMDKRREKISSR
                     LKEADDKKTEAEKLREEYENKKKELEGKREEIISDAKEEAEKRKKQMLHESREEIEQL
                     RQDWQKSLEHEKSSILNTLRDRTARQVFEISRQVLDDLAGAELQEQMIDRFLERFSEL
                     SQKQLDKLKSSVSGNKGQVQVLSSFEISGKTKEKLTRALKDKLDGIRDIDYEEDSDLR
                     CGIALVAEGQRIEWNIDDYLEGLEEEFKERMVELKAEKVKGQDKPDGEDDNKKPEEQE
                     PEKPSQSDK"
     gene            3955..>5066
                     /locus_tag="ENO07_07210"
     CDS             3955..>5066
                     /locus_tag="ENO07_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015226541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDS01793.1"
                     /translation="MSPEDTGLKSIIDHTLSLLESTRREYKYNLEMYESGRTVAVISS
                     IAWVRGLRSVGFQELVRFPGGLRGIVFNLKPRQTGIILLDRGADIPVASEVKRSHRVI
                     DVPVGEELIGRVVDAIGRPLDGGGPVNTENRMPVEREAPSMMDRDPVKKPLLTGIKAI
                     DALIPVGRGQRELILGDRKTGKTAIALDTIINQRDQDVICIYCAIGKKSSDIASVIED
                     LRQHDALDYTIVVAASGENPPGLKYIAPYSATSMGEYFRDRGRDALVVYDDLTLHARA
                     YRELSLLLRRPPAREAYPGDIFYIHSRLLERATHLNPELEGGSLTALPVIETQAQNIS
                     AYIPTNLISITDGQIYLSPKLYRQGILPAIDIGKSVS"
BASE COUNT         1254 a         1158 c         1405 g         1249 t
ORIGIN      
        1 atcctgagct atgtgataga tgatcaggaa gaattcctgg ccatagatga gggtattctg
       61 gtcaagcgcg gctcgcaggt aatgatttcc acctataaag cgatcaaaga taggcccctg
      121 ggagagctgc agaaggcaat tgacgaggaa attacggtat tgagcgagca tgagagaaaa
      181 gcccgctcct ctctggcaat gctggaggga ggaattatac gcaaattcac tgagcagagt
      241 cagattgaat aaaagggaaa tatgagcggc aaatttggcg agaagatcag caaaaaagaa
      301 aaacgcaagc tgaaagcccg gagggaaaaa gacaaatcgg gcctgtggtt ctggctgggc
      361 atgttcggta tggtgggatg gtcggtggcg atccccacca tagtgtgcct ggcgatcggc
      421 atctggatcg attccaccca tccgggacag atctcctgga ccctgacctt tctggtgatc
      481 ggagttgtgc ttggatctct caatgcctgg tactggatca aacgggagag caggcatgac
      541 tgaatcgtca gacattattc tgcttacctt tgtggtgggg ctgatcctcc tggcggtgcg
      601 cctctttggg tcatatctta agcggctggg actgcctgcc ctggtggcct tcatcttgat
      661 aggcgtaatt ctgcgggcca gccattacca gtggcactgg ataacatccg agtgtgagga
      721 tgtcttcgag tttctggcaa aactgggact ggtcgccctt ttgttccgga tcggactgga
      781 aagcaacatc aagatgcttt ttgagcgtct caagagcgcc agttttatct ggatcggcaa
      841 tatagtcata agttttctgg caggttactg gacggcatat tacttactcg atttgaatct
      901 ggtccccagc ctctttatag gatcggctat ggtagcgaca tcagtgggta tatcggtcgg
      961 catctggaaa gaggctgagc agctcaaatc gaggcgagga gagatattga ctgatgtggc
     1021 cgagttggat gatatcaccg gtattatagt gatagcggtg ttattttcta ttgtaccgtt
     1081 cctgaaacag ggctctttct cgggagcgct gttttcagaa gttttacggg agaccggtct
     1141 ggtgctattg aagcttgtat tattcggggc gatatgtgtt ttattctcta tttatctcga
     1201 gcaacgtgtg gtcagttatt tcaaaaatct gcagaaaaaa tcggaaccga tcatagtaat
     1261 tgccggactc ggaatagtcc tggcatcgct ggccggcctc cttggatttt cattggcaat
     1321 cggggctttt tttgcggggg cagccttctc ccgtgacccg gaacatgtca agatcgatgc
     1381 cgccttttca cctctatatt catttttaag ccctttcttt tttataggga tcggccttaa
     1441 ggtcgctctg gcatcccttt ccggggcgat tattgtgggg ctcatcctaa cagtagcagc
     1501 gatatttgga aagataatcg gtacatgcac gccggctgtt ttcggcgaag gctggaaggg
     1561 agcaagcctt ttaggagtga gcatgattcc gcgggctgaa atcgcactga ttatcatgca
     1621 gaaaggtctg gcactcgggg cctgggcggt ttcatcaaaa ctgttctctg ccatggtatt
     1681 tgtttcggcc gcgacatgta tcctgacgcc tttcgcagta agagccatgc tgcataaatg
     1741 gccccaggca caaaccggag gtgcgggctg atacatatat cattacagat tataatcggt
     1801 tttgcaatcg gcctggtatt gggcgcattc aattacagcc tgctctggat gacagtaaac
     1861 aggctgatga agagcggcag tcccggcctt attacgctat tcagcttttt cgtacgaatg
     1921 gctgtaatac tgctggtgtt ctacatcctt gcggtcagag ccggatggat ttcagtggct
     1981 gccggtcttg caggcttaat tctaatccgt gcagttatgg ttggaaaatt cggtcccggg
     2041 cagcaatcgt acaggacaaa aggagatcgt cgagtatgac cataagtccc gattccataa
     2101 tatactggga atggaaattc atcaccctga atgccacgat tgtattcacc tgggtggtga
     2161 tggctattct ggtattgatt tccatacttg tcaccagaaa ccttaaatcc ggatttaaga
     2221 tgtcccgctg gcagagcttt cttgaaactg tggtggaata tatccgggac cagatcagac
     2281 aaatagtcag agaggatccg aagagatacc tgccatttct gggcaccctg tttatgttta
     2341 tttcattgtc caatttactg gcattcgtac ccgagtatca tccgcccacc ggttcaattt
     2401 ataccaccgg cgccctggcc atctgtgtct tctttgcggt acctattttc ggcataagcc
     2461 ggcgcggagt gggaggatac ctgaagcact atctcaaacc taccccgatt atgtttccct
     2521 tcctggtgat ctccgagttt tcgcgcacac tggctctggc agtccgtctt tttggcaatg
     2581 tactcagcgg cacgatgata gctgctgtcc ttctggcggt agcgccgctt tttgtaccgg
     2641 tggtcatgca ggtgttgagt cttctgatcg ggcagattca cgcctatatt ttcgccatac
     2701 tggcggcggt atatatcggt tcggcctccc gggcgcgttc cgaggaggaa catcaaaatg
     2761 atgaaaataa tcaaacagaa aataaagacg aggaggataa ctgatggaca gcataagtat
     2821 tatcggcgca gcctcggttt taggtgcggg gctggctatc gctatcggct cgatcggacc
     2881 ggctcttgga gaggggcgcg ccctggcacg ggcgctggat tctatcgccc agcagccgga
     2941 tgagtccaat acgatcaccc gtactctttt cgtgggtatg gccatgatcg agtcgaccgc
     3001 gatctactgt ttcgttatag cgattattct gattttcgcc aaccccttct ggaactatgt
     3061 aaccgcgcag gcgggaggac aataagatgc agatagactg gtttacattg atcgcacaga
     3121 tcgtaaactt tttgattctg gtctggctcc tgaagcattt tctctacgac cgcatagtgg
     3181 acagcatgga taaacggcgt gagaaaatat catcccgcct caaggaagcc gatgataaaa
     3241 aaaccgaagc ggaaaaactg cgcgaggaat acgagaataa aaagaaggag ctcgaaggaa
     3301 agcgggagga aatcatatcg gatgcgaaag aagaagctga aaaacgaaag aagcagatgc
     3361 tgcatgaatc cagggaggaa atagagcagc ttcgacagga ctggcagaaa tccctggaac
     3421 acgagaaatc ctcgattctt aatacgctca gagaccgcac tgcccggcag gtctttgaaa
     3481 tctccagaca ggttctcgat gacctggccg gcgctgaact gcaggagcag atgattgacc
     3541 gcttccttga acggttctcg gaactgagcc agaaacagct ggacaaattg aaaagttcgg
     3601 tcagcggcaa caagggccag gtgcaggtac tgagttcttt tgagatttcc ggcaagacaa
     3661 aagagaagct gaccagggcc ctgaaagata agctcgatgg gatcagggat atcgattacg
     3721 aagaagacag cgacctgagg tgtggaatcg cactggtggc ggaaggccag cgcatagaat
     3781 ggaatatcga tgattatctt gaagggcttg aagaagaatt caaagagcgg atggtcgaac
     3841 taaaagccga gaaagtgaag ggccaggata aacctgacgg tgaagacgat aataaaaagc
     3901 cagaagaaca ggagcctgaa aagccctccc agtcggataa gtgagtgatt cattatgagc
     3961 ccagaagata ccggtttaaa atctatcatc gaccatacat tatcactgct tgagagcacc
     4021 cgcagggaat ataaatacaa cctggagatg tatgagagcg ggcgcacggt cgcggtaatc
     4081 tcgagtattg cctgggtacg ggggttgagg agcgtcggat ttcaggagct ggtcaggttt
     4141 cctggagggc tgcgaggaat agtattcaac ctcaagccgc gccagacagg tattatcctg
     4201 ctggacagag gcgcagatat cccggttgca agcgaggtca aacgatctca tcgtgtgatt
     4261 gatgtgcctg tgggtgaaga gctgatcggg cgggtggtcg atgcgatcgg ccgtcctctt
     4321 gacggaggcg gtcccgtaaa cacggaaaat cgtatgccgg tcgagcgcga ggcgccctcg
     4381 atgatggatc gtgaccctgt caaaaagccg ctgctgaccg gaatcaaggc aatcgatgcc
     4441 cttatcccgg ttggtcgagg tcaacgagaa ctcattctgg gggaccgcaa gacgggaaag
     4501 acagctatag ctctggacac gataattaat cagcgcgatc aggatgtcat ctgcatctac
     4561 tgcgctatcg gcaagaagag ttcggatatt gcatcggtaa ttgaggacct gcgtcaacac
     4621 gatgctcttg attatacgat tgtggtggct gcctccgggg aaaatcctcc cggcttgaaa
     4681 tatattgctc cttattccgc cacttcgatg ggagagtatt tcagggaccg gggccgtgat
     4741 gcgctggtgg tatacgacga cctgacgctg catgccaggg cataccgtga gctgtcctta
     4801 ctcctgcgcc gtccgccagc gcgcgaggca tacccgggcg atatctttta tattcattcc
     4861 cgtctgctgg agcgggcaac ccatttgaat ccggagctcg aagggggctc tttaaccgca
     4921 ctgcccgtaa tagaaactca ggcgcagaat atctcggcct atattcctac taatttgatt
     4981 tccattaccg atgggcagat atatctgtcg cccaagttat atcggcaggg aattcttccc
     5041 gcgatcgata ttggcaaatc ggtctc
//
LOCUS       DSEB01000397            1744 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1013708, whole genome shotgun sequence.
ACCESSION   DSEB01000397 DSEB01000000
VERSION     DSEB01000397.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1013708"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            156..782
                     /locus_tag="ENO07_07215"
     CDS             156..782
                     /locus_tag="ENO07_07215"
                     /inference="COORDINATES: protein motif:HMM:PF00081.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDS01794.1"
                     /translation="MAYEWKFNPRPYSDDEAKKLLKNVVSPETTDWHYNTHHKGYVTF
                     LNKIEQALEKADVDAANGNWSEFGELKRRLTWNHSGALLHDIYWEVLGGDGDTSKGPD
                     IVSAIEKEFGSIENWKKDFKQTAFAAKLSGWGLLVWDQLYSGRLMNVLVDEHQYGAIW
                     GGIPLISLDVFEHAYYHKDGPGRAKYIDNFMDNLHWGRINDRYKKFVR"
     gene            1233..>1744
                     /locus_tag="ENO07_07220"
     CDS             1233..>1744
                     /locus_tag="ENO07_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDS01795.1"
                     /translation="MHIAIIARELDLGQHQVGATIELLDDGATVPFIARYRKEATGSL
                     DEVQITRIRDRVQQLRELDKRRDAILKSLDERELLTEEIQDKIMAAETMARLEDIYLP
                     YKPRRRTRAIIAKEKGLEPLAEMIFAQENIKIDREAEKFIDPEKEVENIEDALSGARD
                     IIAEWINEDMT"
BASE COUNT          479 a          385 c          465 g          415 t
ORIGIN      
        1 caatagtatt taagcacagt ttatatagat tactatttgg acgggtgcta tgaagatgaa
       61 ttcaaagttt tcgaggtgat cgtgtaatac tatagctcat aaggaattat atcagaaatt
      121 ttgcacaggt ctacaataaa aaaaggagag taatcatggc ttatgaatgg aaatttaatc
      181 cgaggcctta ttccgacgat gaggccaaaa agcttttaaa gaatgttgtt tccccggaga
      241 cgactgactg gcactacaac acacatcaca agggctatgt cacatttctc aacaaaattg
      301 agcaggctct ggagaaggcc gatgttgatg cggccaacgg caactggtcc gagtttgggg
      361 aactcaagcg caggctgacc tggaatcatt ccggtgcgct tttgcatgat atatactggg
      421 aagtcctggg tggtgatggt gatacctcta aaggtcccga tattgtctct gcaatcgaaa
      481 aggaattcgg cagcatcgaa aactggaaga aggatttcaa acagaccgct tttgcggcca
      541 agctttccgg atggggactt ctggtctggg accagctcta cagcggcagg ctgatgaatg
      601 tgctggtcga tgaacaccag tacggcgcca tctggggcgg cataccgctg atttcactgg
      661 acgtcttcga gcatgcctat taccacaagg atggcccggg ccgtgccaag tatatcgata
      721 attttatgga taatttacat tggggccgaa tcaacgaccg ttacaagaaa tttgtaaggt
      781 agttgatttg aaacaatgta aaagtgggct acttaattcc cggcatccgg cttcggccgg
      841 atgctttttt ttgcctcgca cgaaataaac cgggtggtgg cggcctgccc aatctgagtt
      901 ccggcaggcg gcagttttga tcacgcccaa agcgagtttg ggcgtgctac ccaggattca
      961 gggcgtgttg aaaaaggtac ttttgtcgcg cagggtcttg gcactcgatg gaatctgggg
     1021 gtgtgaccct gcgccttatt atatagcagg catttgcgtt gggtcacaat tccgtctgag
     1081 aaggaatcaa gacccaacgc tacgccatca gggtttatca acaagccctt tagattacgt
     1141 atgtaaacgc aatagcttga ccaaaaggca attatcaatt attttcatcc tcatcagaat
     1201 tcgaggagac agctttggaa gatcgtccgg ctttgcacat agccataatt gcgcgtgaac
     1261 ttgacctggg gcagcatcag gtgggcgcga caattgaact tcttgatgac ggcgccactg
     1321 tgccgttcat agctcgttac cgcaaggagg ctaccggctc actggatgaa gtgcaaatta
     1381 ccagaatccg tgaccgggtt cagcagctgc gggagctgga caagcgccgc gatgcgatac
     1441 tcaaatccct ggatgagcgc gagcttctga ccgaggagat acaggacaaa ataatggccg
     1501 cagagacaat ggccagactc gaggacatct accttcccta taagcccagg cgccgtacac
     1561 gggcgatcat cgcaaaagaa aaaggtctgg agccactggc cgagatgatc tttgcgcagg
     1621 agaatatcaa gatagataga gaagcagaga aatttatcga tcctgaaaag gaagtcgaga
     1681 atatcgagga tgctctttcc ggcgcacgcg atatcatagc cgagtggatc aatgaggata
     1741 tgac
//
LOCUS       DSEB01000398            1657 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1015315, whole genome shotgun sequence.
ACCESSION   DSEB01000398 DSEB01000000
VERSION     DSEB01000398.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1015315"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..490
                     /locus_tag="ENO07_07225"
     CDS             <1..490
                     /locus_tag="ENO07_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HDS01796.1"
                     /translation="TMTAVGRAAFKMVHEVRRQFREIPGLMEGKAKPDSPRCVAIATT
                     GALQEMIIPGLLAVIVPVIVGLTLGAYALGGLLAGATLTGVLMALMMSNGGGAWDNAK
                     KYIEAGNLGGKGSEAHKAAVVGDTVGDPFKDTSGPSMNILIKLMAMVSLVLASAGVFT
                     KL"
     gene            626..1348
                     /locus_tag="ENO07_07230"
     CDS             626..1348
                     /locus_tag="ENO07_07230"
                     /inference="COORDINATES: protein motif:HMM:PF04893.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01797.1"
                     /translation="MHDIYSEIVEPRRKAGLRQVAEMLTSPTSAFRSIVESGHVWLYP
                     FVVLSLVLVLSMLVQLPAVDDAAQESVLLGMDQEKLDAAITVMAYVFIFIFVPIFALA
                     AIVLPALIFMFFENFVLGGRADYRQVLNVTAYAFMPSALGIILTSIISWAFQVPEFSF
                     SPAVLLTEEMRHTFMGNWLGIFNVFTIWSLLLTTIGLSVLYGHSRVRTAAWLFPLYLV
                     VFTFFGLFGSFMESFSSDMMAA"
BASE COUNT          387 a          374 c          429 g          467 t
ORIGIN      
        1 aaccatgacc gcggtcggac gtgccgcgtt caagatggtg catgaagtca ggcgccagtt
       61 cagggaaatt cccggcttga tggaaggtaa ggccaagccc gattcaccgc gctgtgtggc
      121 aattgccacc accggtgcgt tgcaggaaat gatcatcccc ggcctgctgg cggttatcgt
      181 gccggtcata gtcggcctga ctctcggagc atacgctctg ggcggtctcc tggcgggcgc
      241 gaccttaacc ggtgtgctca tggcgctgat gatgtctaat ggcggcggcg catgggataa
      301 cgccaagaaa tatatcgagg ccggtaatct gggcggcaaa ggttccgaag ctcataaagc
      361 agccgtcgtg ggcgacaccg taggtgaccc cttcaaggat acatccggcc catccatgaa
      421 tattctcatt aagctcatgg ctatggtttc tctggttctg gccagcgccg gtgttttcac
      481 gaaattataa gaatcgatgc ctgatataag atacaaaggc catccatccg ggtggccttt
      541 tttgtattgc cgcgccctaa aagaacaaca ttttcattga cattagccat catttttcct
      601 atctgatttt agcctggagg tgaatatgca cgatatctat agcgaaatag tggagccgag
      661 aaggaaagcc ggtttgcggc aggtggccga gatgctgacc tcacccacga gcgctttcag
      721 atcgattgtc gaatcggggc atgtctggct ctatccattt gtagtcctgt ctttggtcct
      781 ggtgttatcc atgctggtgc agcttccggc agtagacgat gcggcgcagg aatcggttct
      841 tctgggaatg gatcaggaaa aattggatgc cgctataacg gtcatggcct atgttttcat
      901 attcattttt gtgcccatat ttgccctggc ggcaattgtg ttaccggcgc tcatatttat
      961 gtttttcgaa aattttgtac tgggaggcag agccgattac cggcaggtat tgaatgtgac
     1021 ggcatatgca ttcatgccct cggcgctggg gattattctg acctcgatca tatcatgggc
     1081 gttccaggtt cctgaatttt cattcagccc ggctgttctg ctgacagaag agatgcgcca
     1141 cacttttatg ggtaactggc ttggcatttt caatgttttt acaatctgga gccttcttct
     1201 gaccaccatc ggactgtccg tattatacgg acacagccgg gtccgaactg cggcgtggtt
     1261 gtttcccctg tatcttgtgg ttttcacttt ctttggcctg ttcggttcgt ttatggagag
     1321 cttctcctcg gatatgatgg cggcttgaaa attttatgaa taaaatatcc gtttgatgca
     1381 aggaatttat gtttggtctg tctattttat tgtatgcggg attatttaaa cggattcaga
     1441 cgggtttgaa tattgaaaca aatgggcata atatacgtca tttattagga aaatgttcat
     1501 acgaagccgg ttataccgac gaaagggcaa tggtttgctt ttaatcaatc ccgaaattga
     1561 aatggaacac gcctcaggat aagatcaaaa tatgctccat ccccatcaaa aaacgaaaag
     1621 aatattcctt tgcaacatac aggaggtaat aatgagc
//
LOCUS       DSEB01000399            3834 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002378, whole genome shotgun sequence.
ACCESSION   DSEB01000399 DSEB01000000
VERSION     DSEB01000399.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002378"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1130
                     /locus_tag="ENO07_07235"
     CDS             <1..1130
                     /locus_tag="ENO07_07235"
                     /inference="COORDINATES: protein motif:HMM:PF13549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01798.1"
                     /translation="LGLDLPDYAASTQKKIRPILPPDTPIGNPLDLIAGAGEKEFQHA
                     LHAVCHDKNIDSVFAIFVPPITVNQEAVAHSIVDTFKEHDKPMVACFMGVGLESTGLQ
                     ILRNNEIPSFIFPESAARTLANLEKYRQWKDRPEGEFKKFEVKKKKVQNIINKAGGNE
                     IIGEQALDILEAYGFPIAPYVYAFSKEEAVKQARKLGFPVAMKVNTPKILHKTDKGVV
                     ALDLRTPDEINKTFDSLKKRVAGDLKSKEKFSVVIQEMVSSGVETVIGMSTDPAFGPI
                     IMFGLGGIYVEIMKDVAFKINPLSDLDAEEMIKSLKGYQILKGFRGSEPVDMDVLIDS
                     ILRISQLVSDFAEIIELDVNPFIISGSPKTTKAVDARFIIK"
     gene            1289..2023
                     /locus_tag="ENO07_07240"
     CDS             1289..2023
                     /locus_tag="ENO07_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HDS01799.1"
                     /translation="MDDARRNFLKGAGGAIVTAGIAGAPFISSQAQAQNAMAAAGKLT
                     EHKLPDLDYAYNALEPYIDEQTMRLHHDKHHHGYVNGLNNAEKQLALARGSGDYDMVQ
                     HWSQMAAFHGAGHYLHSIFWKVMAPPNNGGGGTPPGMIAEKIKEDFGSFENFKGQFSA
                     ASKSVEGSGWGILSYRLRDQRLIILQAENHQKLTQWVDTPLMCIDVWEHAYYLKYQNK
                     RGEYVDNWWNVVNWPQVEEHLRLAMK"
     gene            complement(2086..2991)
                     /locus_tag="ENO07_07245"
     CDS             complement(2086..2991)
                     /locus_tag="ENO07_07245"
                     /inference="COORDINATES: protein motif:HMM:PF12697.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDS01800.1"
                     /translation="MTGERQDFIQTQQRMLDRYRIKAQSQLIEIPSIGGHAQVLVSGE
                     GPPVVVINGIGTPGAMWAPLMAELEGFQLYAVDLPGYGLTDTHPDFASDMRRNAVLFL
                     EEVLGSLGLDRPAFMANSLGSLWSYWLALDRDRVGPMIHIGCPALVLNSSAPFPMRIL
                     SVRPLGRLMTRLQPPSEKQVRQLSKMVKEYPLVPELIDLLVATERLPAFRQMFLSTVH
                     TLLRLRGTRPEKRLTANQLTKIHQPTLIFWGENDPFGSPDIGQAMVESMPDAELHIVG
                     GGHAPWLTQAKRISPIAGEFLRRHS"
     gene            complement(3039..3536)
                     /locus_tag="ENO07_07250"
     CDS             complement(3039..3536)
                     /locus_tag="ENO07_07250"
                     /inference="COORDINATES: protein motif:HMM:PF02915.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01801.1"
                     /translation="MVQMLDNPTIQKSIELAIKTEEIGARFYEELAERFKDEPEIRDI
                     FARLAEDEKTHEKQFKELLGKVPKDKDIDPEREVYLKAASVSEFFKTDAFKGIAKVET
                     AEEVIQRAFRFEKSTLFYYQALKDVIGESEELDAIIEMEKQHMTNLMKVMLNDAKFRG
                     TLDTF"
BASE COUNT          994 a          891 c          949 g         1000 t
ORIGIN      
        1 acctggggct ggatctgccg gactatgccg ccagcaccca gaaaaaaatc cgacccatac
       61 ttccgccgga tactcccatc ggcaatccgc tggatctgat tgcgggagcg ggggaaaagg
      121 aattccagca cgcgcttcat gcagtctgcc atgataaaaa tatagattcc gtctttgcga
      181 ttttcgtacc gcccattact gtcaaccagg aggcggtggc gcattcaatc gttgacacat
      241 tcaaagaaca cgacaaaccg atggtggcat gttttatggg tgttggcctg gagtccaccg
      301 gactgcagat tcttcgtaat aacgaaatcc cgtcattcat attcccggaa tctgccgcac
      361 gcacattggc caatctcgag aaatatcgtc aatggaagga ccgtcccgag ggtgagttca
      421 aaaaattcga ggtgaaaaag aagaaggttc agaatattat aaataaagcc gggggcaacg
      481 agataatcgg cgagcaggcg ctggatattc tggaggccta tggcttcccg attgctccct
      541 atgtttacgc tttttccaaa gaagaggcgg tcaagcaggc caggaagctg ggtttcccgg
      601 tggcgatgaa ggttaacaca cccaagatcc tgcacaagac ggataaaggc gtggttgctc
      661 tggacctgag aactcctgat gagattaaca agaccttcga cagcttgaag aaaagggtcg
      721 ccggggacct caaaagcaaa gaaaaattct ctgtggtgat acaggagatg gtttccagcg
      781 gagtcgagac tgttatcggc atgtccaccg acccggcctt cggcccgatc attatgtttg
      841 gcctgggcgg tatttatgtc gagattatga aagatgttgc ctttaagatc aatccgctat
      901 ccgacctgga cgcagaggaa atgataaaat cgttgaaggg gtaccagata ttgaagggtt
      961 tccgtggaag cgagcctgtc gatatggatg ttttgattga ttcgattctg aggataagcc
     1021 agctcgtaag cgattttgct gaaattattg agcttgatgt caatccattt ataatttccg
     1081 gatcaccaaa aactaccaag gcagttgacg ccagatttat tataaaatag aaaaattctg
     1141 cgacttgatc ctatgggtct cccattcttc cgagtgagag gcccttttgc tatatccaat
     1201 aagattgaac aatggagatt gcatttctgt tttactaatt taagcaaaat caattttaaa
     1261 aaattgacta agaacaaaag gagaagctat ggatgacgct cgcagaaact ttttgaaggg
     1321 agccggcggc gctattgtga cggcgggaat tgcaggtgcg ccgtttatat catcacaggc
     1381 ccaggcccag aacgcaatgg cggcggccgg gaaattaacc gaacacaaac tgcccgatct
     1441 ggactatgcc tacaatgcac tggaaccgta tatagatgag cagacaatga ggctgcatca
     1501 tgacaaacat catcatggat atgtcaacgg tctcaataat gctgagaagc agctggcctt
     1561 ggcccgtggc agcggcgatt acgacatggt tcagcactgg tcacagatgg ccgcttttca
     1621 cggtgcgggg cactacctgc attcgatttt ctggaaagtt atggcgccgc ccaataatgg
     1681 cggaggcggg actcccccgg gtatgatcgc tgagaaaatt aaagaagact tcggatcgtt
     1741 tgagaatttc aagggacagt tttctgcggc ttcgaaatct gttgagggat ccggctgggg
     1801 aattctgagt tatcgtctaa gagatcagcg tctgataata ttacaggcgg aaaatcatca
     1861 gaagctgacc cagtgggtgg atactcccct gatgtgcatc gatgtctggg agcatgctta
     1921 ttatctgaaa tatcagaaca agcggggcga gtatgtcgat aactggtgga atgtggtcaa
     1981 ctggccccag gtggaagaac accttaggct ggcgatgaaa tagattttct atgggaatag
     2041 tagagtttta tcaatatttg aacaatcagt caaaaagata gctggttatg agtgccggcg
     2101 taaaaattct ccggcgattg gactgatgcg tttagcctgc gttaaccacg gggcatgccc
     2161 tcctcccacg atatgcagtt cggcatccgg cattgactca accatcgctt gtcctatgtc
     2221 aggggaaccg aaggggtcat tctcacccca aaatatcagt gtcggttggt gtattttagt
     2281 gagctgattg gctgtcagtc ttttctccgg ccgggtgcct cgcaaccgca gcagggtgtg
     2341 gactgttgaa agaaacatct gacggaaagc tggtaaccgt tctgttgcaa ccagaagatc
     2401 gattaactcc ggaacaagag gatattcctt caccatttta cttaattgcc tgacttgttt
     2461 ttcagaaggc ggctgaagcc gggtcatgag tcggcccagc ggcctgactg aaagaatacg
     2521 cattggaaag ggagcggaag aattgagaac cagcgcggga catccgatat ggatcattgg
     2581 tccgactctg tctcgatcca gggccaacca gtatgaccag agcgatccga gcgaattggc
     2641 catgaatgcc ggccggtcaa gcccgaggct gccgagcact tcctcaagaa agagaaccgc
     2701 gttgcgccgc atgtcgctgg caaaatctgg atgagtatct gtaagaccat atccgggcag
     2761 gtcaaccgca tacaactgga atccctctaa ttcagccatc aagggagccc acattgcacc
     2821 gggcgttcca atcccattta tcacaaccac cggcggccct tcacccgaaa ccaatacctg
     2881 tgcatgtccg cctatcgacg gtatttcgat aagttgagat tgagccttga ttcgataacg
     2941 gtccagcatt cgttgctggg tctgtataaa gtcttgtctt tcgcctgtca tgataaaaag
     3001 accactctcc gatagagccc tgctttatat tttgcaaatc agaaagtgtc cagcgtcccg
     3061 cgaaacttgg catcattgag catgaccttc atcaggttgg tcatgtgctg tttttccatc
     3121 tcgatgatgg catcaagctc ctcgctttca ccgataacat ctttcaatgc ctgataataa
     3181 aatagtgtgg acttctcgaa tctgaatgct ctctggatga cttcctccgc tgtctcgact
     3241 ttggcaattc ccttgaaagc gtcggtctta aagaactccg aaaccgaggc agccttcaga
     3301 tatacctcac gttcaggatc aatatcttta tcttttggca cctttccgag aagctccttg
     3361 aattgtttct catgagtctt ttcatcttct gcaagccggg cgaaaatatc tcggatttca
     3421 ggttcatcct tgaatctctc tgccagttcc tcataaaatc tggccccgat ttcttctgtt
     3481 ttgattgcca gttcaatact cttctggatt gtaggattgt ctaacatctg caccatctcc
     3541 tttttttgtt tgtcgccttt ccagtatgta taaggtctaa cctgatacag atgttccgtt
     3601 cagggcggtc tcaattccag ggcttccgac tgcgctggaa tcaatatttc gctttgattt
     3661 gcgagatcat aatcgtataa ttaggctatg aacatggata aagaaccgaa atcgaaaccg
     3721 tcattttttg ctgaattctg gtcctttgtg gctaataata agcgctattg gctgacgccg
     3781 attgtggtta taattctgat tctggcggct tttatacttt tcaccgagag ctcc
//
LOCUS       DSEB01000400            2612 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1005596, whole genome shotgun sequence.
ACCESSION   DSEB01000400 DSEB01000000
VERSION     DSEB01000400.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1005596"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..819)
                     /gene="nadC"
                     /locus_tag="ENO07_07255"
     CDS             complement(<1..819)
                     /gene="nadC"
                     /locus_tag="ENO07_07255"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDS01802.1"
                     /translation="MNFLRFICHGVLLNIQSNTPHFLVKCFFHLTDLRLRLFLGAMKI
                     SKKNLSSIINNALSEDIGSGDITTGAVISKDKPAQGVIRAKQEGVVFGHEAAREVYNQ
                     LDAKAKYTAKARDGARVGKGEVIAEISAGMKTILSGERVALDFLMHLSGIATLTNRFV
                     SAVDGINVKILDTRKTIPGLRLLEKAAVKAGGGENHRIGLFDMFLIKDNHIEAAGSIS
                     KAIESCLQWRGRKKLKIEVETKSIEEIQEALKYKIDRIMLDNMSLDQMKKAVEYI"
     gene            733..2448
                     /locus_tag="ENO07_07260"
     CDS             733..2448
                     /locus_tag="ENO07_07260"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00512.23,HMM:PF13492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01803.1"
                     /translation="MEKTFDQKMRCVRLYIEEHTMTDKTQEVHCKYLGDTEELCWVFQ
                     PDKFDQEELTLSLRETIARCLKCPVFDEAIRRSTGRRKSDKLLTLTLHRLLGQLVDYD
                     TELVSITGSLQKKVEELAVLKSVSEALLKTQDLRKVMLITLTGVTSGEAFGFNRGMVF
                     LVNTDTKTIDGQIGLGHLERDEAPMVWSQISSQKLTFDDLIDRILNGPDLPPNRLTTA
                     IEKISIPLRPEYGILPRTIIDKQSYFVESFEGDIIVDRGLRLIMKDTPFAAIPLIYEG
                     SPLGVILVDNSINHKPITAEDIATLETLANQAASKIENAILQNKLEVRFAELQHIHAL
                     LMDNQKYLVKSERLADLGRLATTVAHEIKTPLITIGGYADRVLKKAEAGQIDLQSMKT
                     MHDEIMRLERICKEMLDYSQKSQLNLEQNDLNQIISETLHLEAGKLKYQNINFETDFY
                     PEKLQLLSDKDRLKQVLYNLIHNAAEAMANGGKIKIQTGRKGDYVFLRIEDNGCGMES
                     DVMQKLFTPFFTNKRNGTGLGLPVSKKIIDDHGGNIVVESEPNKGTAFTINLPCKNEK
                     LGKEL"
     gene            2450..>2612
                     /locus_tag="ENO07_07265"
     CDS             2450..>2612
                     /locus_tag="ENO07_07265"
                     /inference="COORDINATES: protein motif:HMM:PF00072.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDS01804.1"
                     /translation="MSRVLVVEDEPHLLDLYRMELEDEGYHVEVARDGPTALDMAGSF
                     KPDLVILDIK"
BASE COUNT          692 a          625 c          638 g          657 t
ORIGIN      
        1 aatatattca acggctttct tcatctgatc taagctcata ttatcgagca tgatccggtc
       61 gattttgtat ttcaaagcct cttggatttc ctctatggat tttgtctcaa cttcgatctt
      121 cagtttcttt cttcccctcc attggaggca gctttcgata gctttgctga tcgatccggc
      181 ggcctcgata tggttatcct tgatcagaaa catatcgaaa agaccgattc tatgattctc
      241 tcctccgccc gctttcaccg ccgctttttc caataatctc agccccggaa tagtcttgcg
      301 ggtgtccagt atttttacat ttataccatc cacagcggac acaaatctat ttgtaagtgt
      361 cgcaatgcca gagagatgca tcagaaaatc gagcgccacc cgttcaccgg acagaatcgt
      421 tttcatgccg gcgctgatct cggcaataac ctcccccttt ccgaccctgg cgccatctcg
      481 ggcttttgcg gtgtattttg ctttggcatc caactggtta tacacctcgc gggccgcctc
      541 atggccgaag acgactccct cctgcttggc tcttatgaca ccttgggcag gcttatcttt
      601 ggaaattacc gcccctgttg taatatctcc ggaacctata tcctccgaaa gagcgttatt
      661 aataatggag gagagatttt ttttacttat cttcatggct cctaagaata atcgtaatct
      721 taaatctgtc aaatggaaaa aacatttgac cagaaaatga ggtgtgttag attgtatatt
      781 gaggagcaca ccatgacaga taaaacgcag gaagttcatt gcaagtatct gggtgatacc
      841 gaggagctct gctgggtatt tcagccggac aagtttgacc aggaggagct tacactttca
      901 ttgcgggaaa ctatcgcccg gtgcttgaaa tgtcctgtat ttgatgaagc gatcaggcgt
      961 tccaccggca ggcgcaaatc cgacaagctc ttgacattga cgctgcacag gcttctgggg
     1021 cagctggtgg attacgatac cgagctggtt tcgatcacgg gttcgcttca gaaaaaagtc
     1081 gaggagctgg ccgtattgaa atccgtctcc gaggcgctcc tgaaaaccca ggatctcagg
     1141 aaggtcatgc tgattaccct gaccggggtg acctcgggtg aggcttttgg tttcaaccgc
     1201 ggcatggttt tcctggtcaa caccgatacg aagacgatcg acggccagat cggactgggg
     1261 catctggagc gtgacgaggc ccctatggtc tggagccaga tcagcagtca aaagctcact
     1321 tttgacgacc tgatcgaccg cattttgaac ggtcccgacc ttcctcccaa caggctgacc
     1381 acagcaattg aaaagatctc gataccattg cggccagaat acgggatttt gcccaggact
     1441 ataatagata aacagtccta tttcgtggag agctttgagg gcgatatcat agtggacagg
     1501 ggcttgaggc tgattatgaa ggacacgccc tttgcagcca ttcccctgat ttacgagggt
     1561 tcgcccctgg gagtgatcct ggtcgataat tccataaatc acaagcctat caccgccgag
     1621 gatattgcca cactcgagac tctggccaac caggcagctt ccaagatcga gaatgctatc
     1681 ctgcagaata agcttgaggt cagatttgcg gaactccaac atatacacgc acttctgatg
     1741 gacaaccaga aatatcttgt gaaatctgaa cggctggccg acctggggcg tctggcgacc
     1801 acagtggccc atgaaatcaa aactcccctt atcactatag gaggatatgc cgatcgcgtg
     1861 ctgaaaaagg ccgaggcagg gcaaatcgat cttcagagca tgaagacaat gcatgatgag
     1921 ataatgcgat tggaaagaat ttgcaaagaa atgcttgatt attctcaaaa atctcagtta
     1981 aatttagagc agaatgacct taaccagatt atatctgaaa cattacatct ggaagcgggt
     2041 aaactgaaat atcagaacat caatttcgaa acggatttct accccgagaa gctgcaactg
     2101 ctttcagata aagacaggtt aaagcaggtc ctctacaatt tgatacataa tgccgccgag
     2161 gctatggcca atggcggaaa gataaagata cagaccggga ggaagggcga ttatgttttc
     2221 ctccggattg aggataatgg ctgtggcatg gagagtgacg tgatgcaaaa actattcacg
     2281 ccgtttttca ccaacaaaag aaacgggacc ggactggggc tgccggtttc gaagaagatt
     2341 attgatgatc atggcggaaa catagttgtc gagtccgaac cgaacaaggg cacggcgttc
     2401 acgatcaatc tgccatgcaa aaacgaaaag ctggggaagg agttataaaa tgtccagggt
     2461 attagttgtt gaggacgagc cccatcttct cgatctctat cgtatggagc tcgaggatga
     2521 gggctatcat gttgaggtcg ccagggatgg accgacggct ctcgacatgg ccggctcatt
     2581 caagcccgac ctggtgatac tcgatatcaa gc
//
LOCUS       DSEB01000401            6382 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000759, whole genome shotgun sequence.
ACCESSION   DSEB01000401 DSEB01000000
VERSION     DSEB01000401.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6382)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6382)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6382
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000759"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(31..690)
                     /locus_tag="ENO07_07270"
     CDS             complement(31..690)
                     /locus_tag="ENO07_07270"
                     /inference="COORDINATES: protein motif:HMM:PF13419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDS01805.1"
                     /translation="MKNINCILFDFDYTLGDSSEGIIDCISYAFHNLGMPAPDPNEIK
                     KTIGKSLPETFETFTGHRCGKTIKEFRKLFRARADEVMTDKTYLYSTVPTVLEELGDY
                     RLGIVSTKFSYRIREILRREKIEQFFEAIIGGENVSQNKPDPEGLRLALMKLNTSPED
                     SVYVGDSQVDAETAKRAGIKFVAVLSGVTERHQFESYDVLNFIHDLSELPELLANTAS
                     T"
     gene            complement(765..1778)
                     /locus_tag="ENO07_07275"
     CDS             complement(765..1778)
                     /locus_tag="ENO07_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDS01806.1"
                     /translation="MLKYSILSLLSLLILLALPGYAQETIVPDQDLQPLGIAMENYDY
                     PYQVQYLALQIEGQDVRMAFMDIRPPANPNGMTVLLFHGKNFFGAYWEKTIEFLSNQG
                     YRVIVPDQIGFGKSSKPNIHYSFHRLAENTRKLLEVLGVDKVSLVGHSMGGMLAVRFS
                     LMYPETVSKLILENPIGLEDYRLKVPYTPVESAYENILNYTEDGLRDYHRTYYVDWKD
                     EYDEYVMVHYRWTLGGEYPRLAMASALTFEMVYTQPVVYELPRLEMPTLLVIGQEDRT
                     TLGRGTVEPEVLATLGQYPELGKKAAAMIPDSKLVELENVGHIPHFEATERFHDEIIE
                     FLK"
     gene            1797..3224
                     /locus_tag="ENO07_07280"
     CDS             1797..3224
                     /locus_tag="ENO07_07280"
                     /inference="COORDINATES: protein motif:HMM:PF08450.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01807.1"
                     /translation="MGIKAFLLRPINRPKLNKVQSEGINNEICDILYLAAQFFSVYLW
                     YGEQMKHIPITILLLLTAIAAIAAAQAEEKGYTYYQRKSIEAYQNQDIEGFVTNTELA
                     LKHNPHSATMIYNLACGYALLDEVDTALAILKELALMGIDYGIEDDPDLEAVRQRPEF
                     NEVREIYEETLRPINNSKILYEFPRIDLLPEGIAYDSKSGRIFFGSMHYGTIYEIDNN
                     GNLVEFAELENEIPLACLGLEVDTIRNILWAIGSSFNFRQGFVEEYQGTTGVFGFSLE
                     SGAMVKNVVPPHKVPPSGFNDLAVSSSGDVYISGAGALVYRNATGKLDTLIHGDKMVG
                     SNGITLSTDETMLFVADYAGGIVVLELTEGRSRELKCPPEISLYGIDGMYFYEGSLIA
                     IQNAFDPWRLVRFYLNDDYTAIESMTVLEQKNPRAAVAFTGALAGDDFIYVAHGMPPE
                     EIPEYIPAQIRGEIGKAMILQTPLK"
     gene            complement(3331..3741)
                     /gene="msrB"
                     /locus_tag="ENO07_07285"
     CDS             complement(3331..3741)
                     /gene="msrB"
                     /locus_tag="ENO07_07285"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015218866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HDS01808.1"
                     /translation="MLDKSVRSDKKLRAELTPLQYEVTQEKGTERPFSGEYYDFYKDG
                     VYKCVVCGNELFDSGTKYKSGTGWPSFWAPISSENVDLKSDRSLGMVRDEVVCSNCGA
                     HLGHVFNDGPEPTGMRYCINSAALKFEEGEDGDK"
     gene            4131..5384
                     /locus_tag="ENO07_07290"
     CDS             4131..5384
                     /locus_tag="ENO07_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDS01809.1"
                     /translation="MRRRIIVIGGGASGIMAAGQAAHEGANVTLLEKKNIPGRKLRIT
                     GRGRCNLTNIAEVSEFVKHFGKNGKFLRQAFARFFSSDLIDFFNQIGLATKIERGGRV
                     FPESDRAQDVADALLTWIKSQNVRIHTETSINKIITDDDRITGAVTDAKRAIEAEAVI
                     IATGGLSYSLTGSTGDGYRLAKSVGHTIVPTRPSLVPLETGVHFPQHLMGLRLKNVSA
                     SLYVNGRKTAQEFGEMEFTDYGLDGPIVLTLSRNAVEPLEHGSKVEISIDLKPALDHN
                     KLDARLLRDIDRHGKMHFNNLLKELLPMQLIPTCMESVQIVPEKSAHQISAEERKRLR
                     LWLKDFRLNISGHRSYDEAIITAGGISLKEIDPRTMESRLVKGLYFCGEVLDLDGDTG
                     GYNLQEAFSTGWLAGRSAAKADPNS"
     gene            5422..6180
                     /locus_tag="ENO07_07295"
     CDS             5422..6180
                     /locus_tag="ENO07_07295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDS01810.1"
                     /translation="MNLIILTDKDRTDGGSYLLEDHRAEHIRSILQLSEGDSIEIGIL
                     NAGQGRGRITTIDSKQVCIRAEKLEKRDYSALSPHIDLICALPRPQTLKKILLICGMM
                     AVRKLYLIRASRVEKSYYHSPLLNPDNYNSFLIEGLSQGKHTRLPSVEIHERFKPFFE
                     EILPSIEKDEEHTVLRIFPDPESNRTIKAFYDGNARRILLAIGPEGGWVPFEIEMMQE
                     HGFEKCTLGRWILRVEHAVTAALSQIEMIRLEKK"
     gene            6203..>6382
                     /locus_tag="ENO07_07300"
     CDS             6203..>6382
                     /locus_tag="ENO07_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDS01811.1"
                     /translation="MERRNISSGSPYEKAIGFSRAVRIGNVISVSGTGPVADDGSTHA
                     PGDAYGQAQRCLQIIK"
BASE COUNT         1704 a         1539 c         1541 g         1598 t
ORIGIN      
        1 atataggctg caaaaatctg ccgagaccat tcaggtcgag gctgtattgg caagcagctc
       61 ggggagttcg gacaggtcat ggatgaaatt caatacatca tagctctcga attgatggcg
      121 ttcggtcacg ccgctcaaga ccgccacaaa tttgatcccg gcacgtttcg cagtctccgc
      181 gtcgacctgg ctgtcaccaa cataaaccga gtcctccggg gaagtattca atttcatgag
      241 tgcgagtcga agtccctccg gatccggttt attctgtgag acattttctc caccgataat
      301 ggcctcaaaa aactgttcta tcttttcccg cctgagtatt tcccggattc ggtagctgaa
      361 tttggttgag acgataccca gcctgtagtc tcccaattcc tccaataccg ttggtacagt
      421 gctataaaga taagtcttgt ctgtcattac ttcatcagcc cgggccctga acaacttcct
      481 gaattccttt atcgtcttcc cgcacctgtg accggtaaat gtctcgaatg tctcaggcaa
      541 tgatttgccg atcgtcttct ttatctcgtt gggatcgggt gccggcattc ccaggttgtg
      601 aaatgcgtat gagatgcaat caattatgcc ctccgatgaa tctcccagcg tatagtcaaa
      661 atcaaacaat atgcaattaa tgtttttcat gaatcaaccg catccctcgg ttcaagtgct
      721 tcgcaaccat ataatcacaa attatcatcg gacagtccag aacattattt caggaattca
      781 ataatctcat catgaaatct ctctgtggcc tcaaaatgag gtatatggcc gacattttcg
      841 agttccacca gtttgctgtc agggatcatg gcggccgctt tcttaccgag ttccggatat
      901 tggcccagcg tcgccaggac ctcgggttcc acagtcccac ggcccagggt agttcggtct
      961 tcctggccga ttaccagcag ggtcggcatc tcaagccgcg gtaactcgta cacgaccggc
     1021 tgagtgtata ccatctcaaa tgtcagagcc gaggccatgg ccaggcgcgg atattcaccg
     1081 ccaagagtcc agcggtaatg caccatcaca tattcatcat attcatcttt ccagtcaacg
     1141 taatatgtcc tgtgataatc cctgagaccg tcttccgtat aattaaggat attctcatat
     1201 gcactttcaa cgggagtata gggaactttc aggcgataat cctcaaggcc gatgggattt
     1261 tccaatatca gtttgcttac agtctcgggg tacatcaagg aaaacctgac cgccagcata
     1321 ccacccatgg aatggccgac aagcgagact ttatcaactc ccagcacctc caggagcttc
     1381 ctggtattct ctgccagcct gtgaaaggaa taatggatat tcggttttga tgattttcca
     1441 aagccgatct ggtcgggcac gattacgcga tatccctgat tactgagaaa ttcgatggtc
     1501 ttctcccagt aggctccgaa gaaattcttg ccgtgaaata atagcacagt cataccattc
     1561 ggattagctg gaggccggat atccataaag gccatacgca catcctgacc ctcaatctgc
     1621 agggcaagat attgtacctg atagggataa tcataatttt ccatggcgat acccagtggc
     1681 tgcaggtcct gatccggtac gattgtctcc tgagcatatc caggcagcgc gagcagtatt
     1741 aacagagaaa gcagagataa tattgaatat ttcagcataa gctcctcaca tcagaaatgg
     1801 gaattaaggc cttcttatta agacctatta accggcctaa actgaataaa gttcagagcg
     1861 aagggataaa taatgagatc tgtgacattt tatatttggc agcgcagttt tttagtgttt
     1921 atttgtggta cggagaacag atgaaacata taccgataac aatattattg ctgttaacgg
     1981 caattgccgc gatagcggct gcccaagccg aggaaaaagg atacacttac taccagcgaa
     2041 agtcgataga ggcatatcag aatcaggaca tagaaggctt tgttacaaat accgaactgg
     2101 ccttgaagca caatccccat agcgcgacca tgatatacaa ccttgcctgc ggatatgctc
     2161 tgcttgatga agtcgatacg gccctggcta tcctgaaaga acttgccctg atgggaatcg
     2221 actacggcat cgaagatgat ccagatctgg aagcggtaag acaaagaccg gaattcaatg
     2281 aggtcagaga gatttatgaa gagacactgc gtcccataaa taacagcaaa atcttgtacg
     2341 agtttccccg catcgatcta ttacccgaag ggattgcata cgattccaaa agcggcagaa
     2401 tatttttcgg cagtatgcat tatggaacta tctatgagat agacaataat gggaatctgg
     2461 ttgaattcgc tgagcttgaa aacgaaattc cgctggcctg tctgggactc gaagtggata
     2521 cgataaggaa tatcctgtgg gcgattggct catccttcaa cttcaggcag ggattcgtag
     2581 aagaatacca gggtaccacc ggagtatttg gattcagcct tgaatccggc gcaatggtca
     2641 aaaatgttgt ccctccgcat aaggttccgc cctccgggtt caatgatctt gcagtcagca
     2701 gttcagggga tgtctatatt tcaggggcag gcgcgcttgt ttacaggaac gctaccggga
     2761 aactcgacac gttgattcac ggtgataaaa tggttggctc taatggcatc accctctcaa
     2821 ctgatgagac catgctcttt gtggccgatt atgcaggcgg gatagttgtc ctcgaattga
     2881 cagagggccg cagcagggag ctcaaatgcc cgccggaaat atctctatat ggtatcgacg
     2941 ggatgtattt ttatgaggga tcgctgatag caattcaaaa cgcgttcgat ccctggcgct
     3001 tggtcagatt ttatttgaat gatgactaca cggcaattga aagcatgact gttctcgagc
     3061 agaaaaatcc ccgggctgct gtagcattca cgggcgcatt ggcaggtgat gactttatct
     3121 atgtagccca tggaatgcct ccagaagaga tacctgaata tattcccgct cagataaggg
     3181 gcgagattgg caaggcgatg atcctgcaga ccccgctgaa atgaaataac agtaaaatga
     3241 aagaataaaa aggcccgccc agtgagggcg aacctttttg gaaggctatg cgccggagat
     3301 attcggcacc gggattatct ccggctgact ttatttatcg ccatcctccc cctcttcaaa
     3361 ctttaatgcc gccgagttta tgcaatacct cataccggta ggctcggggc catcgttaaa
     3421 gacatgaccg agatgcgccc cgcaattgga acagacaacc tcgtccctca ccatccccag
     3481 gctcctgtca ctcttcaaat ccacattctc actcgaaatc ggggcccaga aactcggcca
     3541 gcccgtcccg gatttatatt tcgtcccgga gtcgaaaagt tcgtttccgc aaaccacgca
     3601 cttatatacg ccgtccttat aaaagtcata atattcaccg ctgaacggcc gttcggtgcc
     3661 cttctcctga gttacctcat attgcagcgg agtcagttcc gctcgcaact tcttatctga
     3721 ccttacggat ttatctaaca tgttgtttcc ctcctctgct gtaattttct gttcatcccc
     3781 tgatcccctc tcagcctgac ctcttaaact gttacctgcg gccaggtata ttaatattcc
     3841 gctcaatgca agaataagaa tcgggagtat tttattggaa atattggatt tcataacttc
     3901 ctcaaattgc gcactgctcc attcagatta aataatctca aaatttcatg aaatcgcccc
     3961 tgccgttctg ttaaacaaaa aaccctttct ttcggttcca taagagagtt gtagcctcac
     4021 agagattgcc ggggcgaaat aattcccgga atgggatcct catagctgtt gactttcaga
     4081 aataatccgg cttattgtcg ctggaatatt gaagagaaca aggagcattt atcaggcgga
     4141 gaataatagt tatcgggggt ggagcttccg ggattatggc ggccgggcaa gcggcccatg
     4201 aaggcgccaa tgtgacactg ctggaaaaga agaacatccc cggcaggaaa cttagaatca
     4261 ccggcagggg gcgctgcaat ctcacgaata ttgccgaggt ctcggaattt gtcaagcatt
     4321 tcggcaagaa tggaaaattc ctgcgacagg ccttcgcacg ctttttctca tccgacttaa
     4381 ttgatttttt taaccagatt gggcttgcca ccaagattga aagaggcgga agggtgtttc
     4441 ctgaaagcga cagggctcag gatgtcgcag atgccctgct gacttggatt aaaagccaga
     4501 atgtgaggat acataccgag acatcaatca ataaaataat cactgacgat gacagaatca
     4561 ccggggcggt cacggatgca aagcgggcaa tagaggctga ggccgtgatt attgcaaccg
     4621 gcgggctttc ttattcactg accggctcga ccggcgatgg ctatcgcctg gcgaagtcgg
     4681 ttggacatac gatcgtgccc actcgtccct cgctggtacc gcttgaaaca ggggtgcatt
     4741 tccctcagca tctgatgggg ctcaggctca aaaatgtaag cgcttccctt tatgttaacg
     4801 gtagaaaaac agcgcaggaa ttcggtgaga tggaattcac cgattatggt ctggatggcc
     4861 cgatcgtatt aacattgagc cgcaatgcag tcgagccgct ggagcacggc agcaaggtgg
     4921 agatatcaat cgatctcaaa ccggcgcttg atcataataa gctcgatgcc cgcttattgc
     4981 gcgatatcga ccggcacggc aagatgcatt tcaataacct gctcaaggaa ctgctgccca
     5041 tgcagctcat tcccacatgt atggagtccg tgcagattgt acccgaaaaa agcgcccatc
     5101 aaatctcagc cgaagaaagg aaacggttgc gtttgtggtt aaaggatttt cgcctgaata
     5161 tcagtggaca tcgttcgtat gatgaagcga ttataactgc gggcggtatc agcctgaagg
     5221 aaatcgatcc gcgcacaatg gagtcgcgtc tggtaaaggg gctttatttc tgcggcgagg
     5281 tgctggatct ggatggcgat accggcggct acaacctgca ggaggcattt tccaccggat
     5341 ggctggccgg aaggtcggct gctaaagctg atcctaattc ataattgaca ttgcggagat
     5401 gtttgacctt atttcctctc tatgaatctt atcatactca cggacaaaga caggacggat
     5461 ggcggcagtt atctgcttga agatcacagg gccgaacata tccgcagcat ccttcaacta
     5521 tctgaaggcg actcaattga aatcggcatc ctgaatgcgg gacagggccg cggtagaata
     5581 accacgatcg actcaaagca ggtgtgcata agagcggaaa agttggagaa aagagactat
     5641 agcgctttat cgccacacat tgacctgatc tgcgctcttc cccgtcccca gactttgaaa
     5701 aagattcttc tgatctgcgg gatgatggcc gttcggaaac tctatctgat tcgcgccagt
     5761 cgggtggaaa aaagttatta ccattccccg cttctgaatc ccgataatta taactctttt
     5821 ctgatcgaag gattgagcca gggaaaacac accaggcttc catcagttga aatccatgaa
     5881 agattcaagc cgttttttga agagattcta ccttcgatag agaaggatga ggagcacacc
     5941 gtgctaagga tttttcctga tccggaatca aatcggacca taaaagcttt ttatgatgga
     6001 aatgccagac gaattttgct tgccatcggg cctgagggtg gatgggtgcc gtttgagatc
     6061 gagatgatgc aggagcacgg tttcgagaag tgcacccttg gccgctggat tctcagagtg
     6121 gagcatgcgg tgacagcggc cctctcgcag attgagatga tacgccttga aaaaaaatga
     6181 gttaaaataa aaggagcaat caatggaacg aaggaatata tcatccggat caccttatga
     6241 gaaagcgatc ggattctcgc gagcggtgag aattgggaat gtaatttccg tctctggaac
     6301 cggtccagtt gctgatgacg gcagcacaca cgctcccggt gatgcctacg gccaggccca
     6361 gcgctgcctt cagattatca ag
//
LOCUS       DSEB01000402            2026 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1009792, whole genome shotgun sequence.
ACCESSION   DSEB01000402 DSEB01000000
VERSION     DSEB01000402.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1009792"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1133
                     /locus_tag="ENO07_07305"
     CDS             <1..1133
                     /locus_tag="ENO07_07305"
                     /inference="COORDINATES: protein motif:HMM:PF00664.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDS01812.1"
                     /translation="IRIVKGFNTDKFEIRKFVKQADDYFRTLLKISRVRLFSNPINEL
                     LGTVAVVIILWFGGRNVLAGTGISAPDFVLFAFAMFSLIAPVKSVSNLHIKIQEGMAA
                     AERIFTVIDSSVEVKDAPDAVEISEFSKEIKFENVSFSYKTTPNVLKNINFTVKKGEL
                     IAIVGPSGAGKSTLCDLIPRFYDPTEGRITIDGHDLRQVKLSSLRAQLGIVTQETMLF
                     NDTIYNNITYGLDGVSKKNVEEATRIANAYDFISAFEKGFDTPIGNRGVMLSGGQRQR
                     IAIARAVLRNPQILIFDEATSSLDTESEQVVQEAIDRLLRGRTTFVIAHRLSTILNAN
                     KILVIQDGGLIDSGTHQELISRKGLYQRLYNLQFKEQNSNGS"
     gene            1123..>2026
                     /locus_tag="ENO07_07310"
     CDS             1123..>2026
                     /locus_tag="ENO07_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HDS01813.1"
                     /translation="MGLDTRTWHGRLLKSIEIGFKRIVLKVFLFFGGVRKARRKFDPA
                     EFKKVLFIRHDKLGDMVISLPIFHNLKKFYPEVQIDVVCGKDNYEIVRTDPHITNIYY
                     YHKKPLRDWKNIQIMKSENYDCVVDLIFGSSVTAVILVSIIAGRRFKLGVGKLDYAKY
                     FDRTVEFERYKYPIIEATGRVLNLFGIDLKDCDMRPRIYLEERDFEAGREFVAELKKK
                     YDTVVCVNLSAGRPGRIWPLEKYFKLIEMLLDEYPEWAFALNYAPSEFWKAEKLLKKY
                     NSRVVPIPEGLNIRQVAGMMKYYDF"
BASE COUNT          536 a          449 c          535 g          506 t
ORIGIN      
        1 atatccgcat tgtcaagggc ttcaacactg acaaattcga gattcgcaag tttgtcaagc
       61 aggccgatga ctatttcagg acgcttctga aaatatcccg ggtacggctc ttttcaaatc
      121 ctataaatga gctcctgggc acagttgccg tggtcataat cctgtggttc ggggggcgta
      181 atgttcttgc tggaacgggg atatccgcgc ccgattttgt gctctttgca tttgccatgt
      241 tctcgcttat agcgcccgtc aaatcggtct ccaatctgca tataaaaatt caggagggga
      301 tggctgcggc ggagagaatc tttactgtga tagacagttc ggtggaggtg aaagatgctc
      361 ctgacgctgt ggaaatatct gaattcagca aggagatcaa attcgagaat gtcagcttct
      421 catacaaaac taccccgaat gtcctgaaga atatcaattt tacggtcaaa aagggagagt
      481 tgatcgcaat tgtggggccc tccggagcgg gcaaatcgac cctctgcgat ttgatcccca
      541 gattttatga tcccaccgag ggacggatta ccattgacgg ccatgacctt cgtcaggtca
      601 agctctcctc attgcgtgca cagctcggca tagtcaccca ggagaccatg ctgtttaacg
      661 atacgattta taataacata acctacgggc tcgatggtgt atcgaagaaa aatgtcgagg
      721 aggccaccag gatcgccaat gcctatgatt tcataagcgc ttttgagaaa ggattcgaca
      781 cgcccatagg caatcgcggt gttatgcttt ccggcggcca gaggcagagg atagccatcg
      841 cccgcgcagt tctgcgcaac ccgcaaatac tgatcttcga cgaggccaca tcatcgctgg
      901 ataccgaatc ggaacaggtg gtgcaggaag ccatagaccg gctcctgagg gggcggacga
      961 cctttgtcat agcgcaccgg ctttcgacta tcctgaacgc aaataaaata ctggtcatcc
     1021 aggacggcgg gctgatagac tcaggcacac accaggagtt gatatcgcgt aaaggtcttt
     1081 accagaggct gtataacctt caattcaagg agcagaacag caatgggtct tgacaccagg
     1141 acatggcacg gccgactttt gaaatcaatc gagatcggtt tcaagcggat tgtgctgaaa
     1201 gtatttctct tctttggagg ggtcaggaaa gccaggagaa aattcgatcc tgcagagttc
     1261 aagaaagtgc tttttatacg gcatgacaag ctgggggata tggtcatttc tctgccgatc
     1321 tttcataatc tgaaaaagtt ctatcccgag gtccagatcg acgtggtctg cggcaaggac
     1381 aactacgaga tcgtcagaac agacccgcac atcacgaaca tctattacta ccataagaag
     1441 cccctgcgtg actggaaaaa tatccagatc atgaagagcg aaaattatga ttgtgtggtc
     1501 gatctgattt tcgggagttc cgtgactgct gtaatcctgg tgtcgattat tgcagggcgg
     1561 aggttcaaac tgggcgtcgg caagctcgat tatgccaaat atttcgatcg cacagttgaa
     1621 tttgaaagat ataagtatcc tataatcgag gccacaggcc gtgtactgaa cctgttcgga
     1681 attgatttga aggattgtga tatgcgtccg cggatttacc tggaggaacg ggatttcgag
     1741 gcaggcaggg agtttgtggc cgagctgaag aaaaaatatg atacggttgt ctgcgtgaac
     1801 ctttcggccg gacgtcccgg caggatctgg ccgctggaaa aatattttaa gttgattgag
     1861 atgcttttgg atgaatatcc ggaatgggct tttgccttga attatgcgcc ctcggaattt
     1921 tggaaagccg aaaagctctt gaaaaaatat aacagtcgtg tcgtaccgat tcctgagggc
     1981 ttgaatatca ggcaggtggc cgggatgatg aaatattatg actttc
//
LOCUS       DSEB01000403            4767 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001465, whole genome shotgun sequence.
ACCESSION   DSEB01000403 DSEB01000000
VERSION     DSEB01000403.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4767)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001465"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..3032)
                     /locus_tag="ENO07_07315"
     CDS             complement(<1..3032)
                     /locus_tag="ENO07_07315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDS01814.1"
                     /translation="MSFAPLHCHSNFSLLYGTIPPLELVRQARRYGLKALALSDHDAI
                     YGLVEFYTHCNEAGIKPLPGAALSTTCGELLCFPENAGGYANLCRLITKRRLGGESLR
                     PEMMAEQAENLIILAPPSQKTAQLKEIFHNKLYLKIAMFKDGSARADIDEALKLSRKF
                     DIKIAAANPVVFLSSEDFELHKVLIAVRYGKTIGGLKPREYAHPESYFRDPCETQNLF
                     RHLPQSLVNIEEIVERINVRLEPHEDIFPEVEIPGGKKPAEYLRELCLEGLKKRGGNL
                     NGRFLSQLDFELGAIEQAGFTTYFLAVADIVGFCKREHIPCVGRGSAAGSLVSYALGI
                     THVDPIENDLYFQRFLNEGRTDPPDIDIDLCWKNRDRVLEYVYQKHGAERVAMICSTI
                     RMQSRMAVREVGKALGVPEADITKLANRLPMAFFSKGKYSKQDFPSLTGIPINSEPYT
                     SIFKYAGRLIDFPRHLGIHPGGICISDGELKAKVALEKSTKGPVVTQLDMYSIDQTGL
                     IKIDLLGQRSLTVIRETANHLGFPDADKIVKPDDNRTYKLLQEGRTLGNFQIESPGMR
                     AMLRDIHPRKLNDITLALSLIRPGATESGAKKLFLERYRNGKKVDYDHPLLEPILKES
                     CGTIIYQEQVLKIAEAVGGFSASDADRLRKAMTKVRSNEQMARIREKFIAGARSKGVK
                     AETAHKIFESMAHFASYGFCKAHAASYALISYQAAYLKAHYPITYMNYVLNNQAGYYH
                     PSVYLEEARRFGASIKPPCVNRSRAECTTDGESIRLGLIFVKSIGEEVITQIEESRKQ
                     EGEFKSLEDFLWRIKIPQGEVKYLIKCGAFDFTGKVRPALLWELSFLYKPILKAKSDD
                     LPPLFNQREKIQKPDRLPELPDYNDFEKWAYEQHILEMSVLKHPLETFGLTKKDAITP
                     RLKNLENQKISTFGWLVDIKRINTSNNSKMFFLTMEDLCDTFEVVVFYDTAKKYSEHL
                     ERYRFFKIEGVIKTEGRNCSIYLTHLEPARTSFAEREY"
     gene            complement(3001..4218)
                     /locus_tag="ENO07_07320"
     CDS             complement(3001..4218)
                     /locus_tag="ENO07_07320"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:PRK02406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDS01815.1"
                     /translation="MEKIILYIDVDAFFASVEQATNPKLRGKPVMVGGYPHERGVVAC
                     PSYEARAEGVYTAMTLSEAYRLVPHGIFLKGDYDNYKNYSDRFCEILKRFSPLVEMIS
                     QDEAVVDLTGCAKDYIEAEKRVQKLKDQISGELSLSTSAGVAQNRFLAKTASEYEKPD
                     GLTVVPAGKEWDFLEKLSISNVPGIGYKSRRILNDMGIKTIGELKQIPQSYLEKLFGQ
                     RGFKMAEFLLNRDPRLLEPVRPIKRISRETGFFGDETDMEFISATLYYLLERACIKLR
                     AVGKKCNRVGIKLRYTGESPVVINRTIPFFHNTEENLLPYAQKLMKRIHTRRLAINLI
                     GVTLSGLKNAATQEELFAEPLQKNDSLIKAIDKLRSSFGYHSLYFGKTLTLKDNYKKL
                     RTGYELRTPSLSQ"
     gene            complement(4218..>4767)
                     /gene="lexA"
                     /locus_tag="ENO07_07325"
     CDS             complement(4218..>4767)
                     /gene="lexA"
                     /locus_tag="ENO07_07325"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDS01816.1"
                     /translation="FISNKGFAPTIREISAGIGIKSPATVQEHLLALERKGVIKRIRG
                     KKRAINIMPEFEAAPENRVPILGQIAAGQPIEAIEDKSSFVEYGQSKPGEELFALRVN
                     GDSMIEDGIFDGDLVVIRKQETCMNGEVAVALLDDGSATLKRIFREKGRIRLQPANSE
                     LDPIYVENIRIQGKVVGLIRRF"
BASE COUNT         1032 a         1287 c         1213 g         1235 t
ORIGIN      
        1 atatactcgc gttcggcaaa cgaagttcga gccggttcca ggtgtgtcag gtagattgaa
       61 caatttcggc cctccgtttt tatgacacct tcaattttaa aaaaacgata acgctccaga
      121 tgttcggaat atttttttgc ggtgtcgtaa aagaccacga cctcgaatgt gtcgcacagg
      181 tcttccatgg taagaaagaa catcttgctg ttgttcgagg tgttgattct ctttatatcc
      241 acaagccatc cgaaggtgct gatcttttga ttttcgaggt ttttcaggcg cggcgtgatc
      301 gcatcttttt ttgtcagtcc gaaggtctcc agcgggtgtt tcaggaccga catttccaga
      361 atatgctgtt cgtaggccca cttttcgaaa tcgttgtaat ctggaagctc cggcagacgg
      421 tccggctttt gaatcttctc acgctggttg aaaagaggag ggaggtcgtc ggatttagcc
      481 tttagaatcg gtttgtacaa gaaagaaagc tcccataaaa gagccgggcg aactttgccg
      541 gtaaaatcga aagctccgca tttgatcaga tacttgacct caccctgggg aatttttata
      601 cgccacagga aatcctccag tgatttgaat tcaccctcct gcttgcggct ttcctcaatc
      661 tgtgtgatta cctcctcgcc aatgctcttt acaaaaatca gtcccaatcg aatgctctca
      721 ccatcagttg tgcactcggc cctgctgcgg tttacacagg gaggtttgat gcttgcaccg
      781 aaacgccgcg cctcctcgag atagaccgag ggatgataat agcccgcctg attattgagg
      841 acataattca tatatgtaat cggataatgc gctttcaagt aggccgcctg atagcttatc
      901 agcgcataac tggcagcatg cgccttgcag aagccgtatg atgcgaaatg cgccatggac
      961 tcaaatatct tgtgtgccgt ttctgctttg acacccttgc ttcttgcacc ggcaataaat
     1021 ttttccctga tcctcgccat ctgctcgttc gagcggactt tggtcatggc tttgcgcaag
     1081 cggtcggcat cggaggcgga gaatccaccc actgcctcgg cgattttcag cacctgttcc
     1141 tggtagataa ttgtcccgca gctttccttc aaaatcggct ccagcagggg atggtcataa
     1201 tcgacttttt tgccgtttct gtatctttcc agaaaaagct tttttgcgcc ggattcggtt
     1261 gctccgggac ggatcaatga cagcgccagc gtgatgtcat tcagcttccg gggatggatg
     1321 tcgcgcagca tggcgcgcat gcccgggctt tcgatctgga agttacccag tgtgcgtccc
     1381 tcctgaagca gcttgtatgt cctgttatca tccggcttga caattttgtc tgcatccgga
     1441 aatcccagat gattggcggt ttcgcggatt accgtcaacg atcgctgacc taaaagatcg
     1501 attttgatca ggccggtctg atcgattgaa tacatgtcca gctgggtgac cacaggcccc
     1561 ttggtcgatt tttccagcgc cactttggcc ttcaattcgc cgtcggagat gcagattccg
     1621 ccgggatgga ttcccagatg gcgcgggaag tcgatcagtc ttccggcata tttaaagatt
     1681 gatgtgtacg gctcggaatt tatgggaatc ccggtcaggc ttggaaaatc ctgcttgctg
     1741 tacttgccct tggagaaaaa ggccatcggc agtcggttgg cgagtttggt gatatcggct
     1801 tccggcacac ccagcgcctt gccgacctca cgcaccgcca tgcgggactg catcctgatg
     1861 gtcgagcaga tcatggccac gcgttcggcg ccatgcttct gatagacata ttccagcacc
     1921 ctgtcgcggt ttttccagca gaggtctata tcgatatcgg gcggatcggt acgtccttca
     1981 ttgagaaaac gctggaagta gaggtcattt tcgatcgggt cgacatgcgt gattccgagt
     2041 gcatagctga ccagagaccc ggcagccgag ccccgtccca cgcagggtat atgttcgcgc
     2101 ttgcagaagc caacaatgtc ggccaccgcc agaaagtatg tcgtgaatcc ggcctgttcg
     2161 attgcgccca gctcgaaatc gagctggctg aggaaacgcc cgttcaggtt gccgccgcgc
     2221 ttcttcagac cctcaaggca gagctcgcga agatattcgg ccggtttttt gccacccgga
     2281 atttcgacct cgggaaagat atcttcatgt ggttccagcc tgacattaat ccgttcgact
     2341 atctcctcga tattgacaag gctctgcggc agatgccgaa agaggttttg agtttcacac
     2401 ggatccctga aatagctttc gggatgggca tactcccgcg gcttcaatcc ccctatggtc
     2461 ttgccgtagc ggaccgcgat caggacttta tgcaattcga aatcctcgga cgacagaaat
     2521 actaccgggt tggcggcggc tattttgatg tcgaatttgc gtgaaagctt cagcgcttcg
     2581 tcgatatcgg cacgagccga gccatcctta aacatcgcta tttttaaata gagcttgttg
     2641 tgaaatatct ctttcaactg cgcggtcttc tgcgagggcg gggccagtat gatcaggttc
     2701 tcggcctgct cggccatcat ctccggccgc aggctttcac ctcccagcct tcgcttcgta
     2761 atcagacggc agaggttggc atagcccccg gcattttcag ggaaacagag cagctccccg
     2821 caggtggtcg aaagcgccgc tccggggagc ggtttgatcc ccgcctcgtt gcaatgggta
     2881 tagaactcga ccaggccgta gatggcatca tggtcgctca aagccagcgc ttttaagccg
     2941 taccgccgag cctgcctgac cagttcgagg ggcgggattg tgccatagag aagcgagaag
     3001 ttactgtgac agtgaagggg tgcgaagctc ataaccggtc ctcagctttt tgtagttgtc
     3061 cttcagggtc agggtcttcc cgaaatagag cgagtggtag ccgaaggatg agcgcagttt
     3121 gtcgatcgct ttgatcaggc tgtcattttt ctgcaatggt tcggcgaaaa gctcctcctg
     3181 tgtggcggcg tttttgagtc ccgaaagagt taccccgatc aggttaatcg ccagccgccg
     3241 tgtatgtatg cgcttcatca acttctgcgc ataggggagg aggttctcct cggtattatg
     3301 aaagaatggt atggtgcggt ttataacgac aggactttcg ccggtgtagc gcagcttgat
     3361 tccgaccctg ttgcattttt tgccgacagc gcgcagcttg atacaggcgc gctccaggag
     3421 ataatacaat gtggcggaga taaattccat gtcggtctca tccccgaaaa atccggtctc
     3481 gcgcgaaatc cgcttgatcg gccgaaccgg ctccagcaga cgcgggtcgc ggttcaaaag
     3541 aaattccgcc atcttgaagc cgcgctgccc gaaaagtttt tccagatagc tctgtggaat
     3601 ctgttttaat tctccgatgg ttttgatgcc catgtcgttc agaatccgtc gcgatttgta
     3661 accaataccg gggacattgg aaatcgacag cttctccagg aaatcccact ctttaccggc
     3721 tggcacaact gtcaggccat ccggcttttc atattccgag gcggtcttgg ccaggaaacg
     3781 attttgcgcc actccagccg aggtcgaaag ggaaagctct ccgctgatct gatctttcag
     3841 tttttgcacc cgtttttcgg cctcaatgta atcttttgcg catccggtca gatcgaccac
     3901 cgcctcatcc tgcgaaatca tctccaccag tggcgaaaac cgcttaagga tttcacagaa
     3961 gcggtcggaa taatttttgt aattatcgta atctcccttc agaaaaatcc catgcggtac
     4021 cagccggtag gcctccgaga gcgtcatggc ggtataaaca ccctcggccc gcgcctcata
     4081 acttggacag gccaccaccc cgcgctcatg cggatagccg cccaccatca ccggcttacc
     4141 cctcaatttc ggattcgtgg cctgctcgac cgaggcgaaa aaagcgtcaa catcgatgta
     4201 caaaataatt ttctccatca aaatctccga atcagcccca ccactttgcc ctgaattctg
     4261 atattttcga catagatcgg gtcgagctct gagttggccg gctgcagcct gatccgccct
     4321 ttttcacgga aaatcctctt caaagtcgcg ctgccatcat ccaggagagc gaccgcgacc
     4381 tcgccattca tacatgtctc ctgtttgcga atcaccacca ggtcgccatc aaaaatgccg
     4441 tcctcgatca tgctgtcacc attaaccctg agcgcaaaaa gctcctcgcc cggttttgat
     4501 tggccgtatt cgacaaagct ggatttgtcc tcgatcgcct cgattggctg accggcggcg
     4561 atttgcccca gtatcggcac tcgattttcg ggggcagctt caaattccgg cataatgttg
     4621 atagctcttt ttttacctcg aatgcgttta atcacgccct ttcgttccag agccagcaga
     4681 tgctcctgca ccgtagccgg ggatttgatt ccgatgccgg cggatatctc ccggattgtg
     4741 ggtgcaaagc ccttattgga tataaac
//
LOCUS       DSEB01000404           14788 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000120, whole genome shotgun sequence.
ACCESSION   DSEB01000404 DSEB01000000
VERSION     DSEB01000404.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14788)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14788)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14788
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000120"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(157..951)
                     /gene="coxB"
                     /locus_tag="ENO07_07330"
     CDS             complement(157..951)
                     /gene="coxB"
                     /locus_tag="ENO07_07330"
                     /EC_number="1.9.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HDS01817.1"
                     /translation="MSFSKLLQAIFFSLSLLVILGLFTALSAEEDITNWLSEPATPLA
                     EEVRGSFIFTLLLILPFLFLAEGLLIFVIFKFRKRPGKEPATFHENVKLEIAWTLAPA
                     ITLVILALSTFTLIKRINYSPHTDLVVQVEAQRFFWKYTYPEYDIAIAEEPLVVPADK
                     YVTLYGTSMDVIHSWWVPAFGVKKDFMPGRITELWFKAKPGVYEGQCAELCGPLHAEM
                     LIDVKVLPEDEFMAWVREKQAEAENIEVRPASAIEQAREDTVDVIN"
     gene            complement(964..1893)
                     /locus_tag="ENO07_07335"
     CDS             complement(964..1893)
                     /locus_tag="ENO07_07335"
                     /inference="COORDINATES: protein motif:HMM:PF13442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HDS01818.1"
                     /translation="MSKIKEKLNLLLYIFMLALAIVLVLKAFFIGYERGEFEGKYYPP
                     REKGPPEEEVEQVNLRALLEPTEELLKEGKQLYQLNCASCHGQNGSGDGPKSAGLNPP
                     PRNFQEEKFKYGASSLEIYNTITKGVTGTSMPAFDLLPEQDRMAMAHYVQTFVPDPPQ
                     NPPELVAELPELDEGEAAPADTAVADTAAVPKTIPLDSARQAYLAQFALAAEAPSGIS
                     INELFEKHCASCHGSYGEGQLRAEQVVPSSVIYLDAGVLAGKDGGKFLNKETFKKFIT
                     NGTPGLPGHNFGFLTENELNDIYNYLQRLSKTR"
     gene            complement(1886..3061)
                     /locus_tag="ENO07_07340"
     CDS             complement(1886..3061)
                     /locus_tag="ENO07_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01819.1"
                     /translation="MKNIGPLDNNGRFDVVLALFFLVGVASFIAGLFVDAQRAWYNYL
                     IEFFFFLSIALGGLVFAAVQYASSAAWSVTIRRIGEGLFSYLPGLAITVAILLLGVKE
                     LYSWAYTAPHDYFTYTKEVYMTTLWFIVREAFFFALWIFFGFMIVNTSLKQDKTRNPR
                     LTRRNKKLSIFFLILFGYTFTFSSMDLLMSLQDHFYSTIFGVYCFSGLFLSGMAAIAI
                     VVITLKNRGYLQNAVEKKHLHDLGTWIMAFSVFMVYIGFSQYMLIWYADLPHEISYLI
                     PRSSNGWQYLFALLPLLKWLLPFIVLMPEKNRSNPRVIFWVSIGVLIGQWLDIYWMVV
                     PSFSESFRMISWIEIGMFLGFFGLYGLVVRRFYKRHSLVSVGDPNLETCLKGRYLHV"
     gene            complement(3102..3692)
                     /locus_tag="ENO07_07345"
     CDS             complement(3102..3692)
                     /locus_tag="ENO07_07345"
                     /inference="COORDINATES: protein motif:HMM:PF13442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HDS01820.1"
                     /translation="MVLKMSMNLKYSILIFGILALLISCGDRETTELQYTPDMYVSPA
                     IKPQEKEPSDSGQIAMRLPPEGTVPRNFEPYQLQATDTLAASELINPIPATEEVLSVG
                     QKYYDIFCYTCHGEAGAGDGPIIPKMTKPPVLYSRKVIDWSDGRIYHLITYGQGNMPS
                     YRNYIDPATRWAIIHYLRVLQKSMNPSGEELATQQQ"
     gene            complement(3658..4176)
                     /locus_tag="ENO07_07350"
     CDS             complement(3658..4176)
                     /locus_tag="ENO07_07350"
                     /inference="COORDINATES: protein motif:HMM:PF11821.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HDS01821.1"
                     /translation="MPESRIIAGFMDDPEQFLEVGSRAREHGYKNLDGVMPYPVHGFE
                     EALGLKKSRVPAAAKLMLVIGAGLGLLFAGWTSAVAWPINVGGKPLFSWQAFIPIVFE
                     CGVLLAGITTFILLFHLGRFYPAKDPKMIRERLTDNQFAVVIPVEDNGNIEDIREFFT
                     KHGIEDVDEFEI"
     gene            complement(4169..5560)
                     /locus_tag="ENO07_07355"
     CDS             complement(4169..5560)
                     /locus_tag="ENO07_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HDS01822.1"
                     /translation="MKSTDIKPLEIEGRKYGGPWISSDITMSDVTDDVMAPVIRKPGV
                     IWIILFMMALAGVGLLGFVLTIQMLKGIGILGLNHPVAWGVYITNFVFWVGIGHAGTL
                     ISAILFLLRQKWRNSINRTAEAMTIFAVICAGLFPLIHVGRTWVAYWLVPYPNARELW
                     VNFRSPLVWDVFAVTTYLTISFLFWYQGLLPDFATLRDRAKNKIQKWFYRILAMGWKG
                     KARDWNHYERAYLQFAWLATPLVLSVHSIVSFDFAVSQLPGWHATIFPPYFVAGAIFS
                     GMGMVVTLLVILRKSMNLEKYITIRHLEMMNKVILLTSMFVGYSYLMEFFVAWYSDEP
                     FEQYVFLNRLVGPYSWAIWTMLICNVFIPLVFFFKKARTSIPIMFAVSICVNIGMWFE
                     RFNIIVTSLHRDFLPSNWGMYTPTWADWSVTLGSFGLFFTLFLLFIKFFPAVSIAELK
                     SILTRPFRRGANA"
     gene            complement(5547..8645)
                     /locus_tag="ENO07_07360"
     CDS             complement(5547..8645)
                     /locus_tag="ENO07_07360"
                     /inference="COORDINATES: protein
                     motif:HMM:PF12838.5,HMM:TIGR04519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDS01823.1"
                     /translation="MTDKKKRYWSTLEEYYHDPEVKRLRREEFFNKPEKAFKQDFGPQ
                     GFSRRDFMKLGGAAAIFSMIACGNRPVEKIVPYVSQPEEITLGIPNYYASTCTACPAA
                     CGLVVKTREGRPIKMEGNPEHPVNKGRLCARGQASLLDLYDPGRLRNPVKIVADGSRE
                     KIEFADADQDIVGRLKNAEGQVVLLTGAYHGPAKLDLVNKFLNIFDNGRHYKYEALFD
                     DGYARSRQQGFGSNKLPQYHLDNADVVVLLGSDLFASGRDRVRHMRDFAEKRKIKDNH
                     MSRVYAFEPAMSEMGSNADERYPVKMSDLPVLAGALMLQLIYMDKRVPGLAQRISADI
                     VRKFDPYEIEEKLGLPMGTIKKISDDLWNNRGRSIILSESFADQGQNSQALHQLVHVL
                     NSLLDNESGTIETDSIPDSHILSGYGDLRQLVNDMNSGRVSAILIAGTNPLYSLPDDF
                     GFAEALKHVPLRISLDDRINETAELCEYVLPGLHGAECWGDSEPYTGLYAIQQPTITP
                     LWNNRQLEDSLIAFGFGLGVQDFSSAEAPIKFYDYVKKYWQNNIYRDAGLAGDFQSFW
                     VKVLQHGFFESKSYKTGGLGATELGIEPVAALGRVNGLSNGPELILSASSIHIDGRSM
                     NNAWLLETPDPVSRIAWDNYLAIAPSRAHEMGLKEGDVVTLTINGKSLDVPVHLAPGT
                     HKDSAVIMVGWGRTSVGDVGNKAGVNAFKLAKSIDSDIHYTLTGVELTKSGKRIPLAS
                     VQGHNYIEHRPILYETTLEEYREDPTAGRHQGHGSQQTLWKKDHRYPGHKWGMVIDLN
                     SCIGCNACMTACQVENNIPVVGKNEVLNGREMHWIRVDRYYSGEENNPDTIHQPMLCH
                     HCDDAPCETVCPVLATVHNDEGLNLQIYNRCVGTRYCSNNCPYKVRRFNYYDYYESAY
                     KEKPLHMLLNPDVTVRSKGVMEKCTFCIQRIRDARHQAKSRGQPIGPDEVQTACQQTC
                     PAGAISFGDLNQKDSLVSKESHQPRAFKALVELNIDPNVSYLTLVRNKDAKKDKHEDE
                     EH"
     gene            complement(8658..9374)
                     /locus_tag="ENO07_07365"
     CDS             complement(8658..9374)
                     /locus_tag="ENO07_07365"
                     /inference="COORDINATES: protein motif:HMM:PF14537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HDS01824.1"
                     /translation="MKVTYFIKKRILLILIVIIFLIIGYNFAANVYNNVGYTPDQPIP
                     FSHKIHAGDNKIDCLYCHHAAEKGPYAGIPAMENCMGCHSVVAIDKENIQQLTRIYSE
                     GKSIEWVRVHRLPDHSYFNHKWHVNAGVECETCHGDVAGMAVIGQVNRLEMGDCIQCH
                     RNSDYSQQYAARLKPGDKASIANNVRWYPYLKYIDSLTLKSAEAKGIEALKKDTLDVE
                     PATGIFPHHNASVQCNICHN"
     gene            9798..10286
                     /locus_tag="ENO07_07370"
     CDS             9798..10286
                     /locus_tag="ENO07_07370"
                     /inference="COORDINATES: protein motif:HMM:PF13668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01825.1"
                     /translation="MSPVSQTVLDGLNEGIKAELAAYVFYMKGMHSTSEQRLKDLLAT
                     LASEEKDHYKILERQYDNLVRSEIWAPYNDIMLQPGLPNIDEQVEDVHDEYIDEITEK
                     TSPMRILEIALMLEKRARDHYAGMAEKVDDPRGKEMFKYLSKFENVHVNKITKMMKEF
                     EK"
     gene            10429..11823
                     /locus_tag="ENO07_07375"
     CDS             10429..11823
                     /locus_tag="ENO07_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015909061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDS01826.1"
                     /translation="MRTEKDSMGEIEIPDDALYGAQTARAVENFKVSGRTGYREFIYA
                     TVQIKKAAAMVHKDLGLLDKEQAEAIVEAADEILAGKYDEHFVVDIFQAGAGTSHHMN
                     VNEVLSNLANKSLGGKLGKYEHVHPNDHVNMAQSTNDVIPTAIRLAALQLLKDFYPAL
                     EETIKLMQHKAKEFDDIIKSARTHLQDAVPIRLGQEFGGYADNLEKHLQSIKTAAVDL
                     KKLGIGGSAAGTGLNVHPKYRQKMAETLSRLIDADLKMADNYFEAMQSMRPFVNLSGA
                     LRGLASDLGRIANDFRLLSSGPRTGLLEINLPAVQPGSSIMPGKVNPVLAEMLNMVCY
                     RVIGNDLTIANAGGAGQLDLNVMMPLIADTLIESLRILTGGLRSFNERCLKNVTANPE
                     RCKAYAENSFAMVTALNTVIGYLKAAEVAKESAKSGKPIKQIVGEKGYLKEEELDKYL
                     SPEKLTEPGIPGKD"
     gene            complement(11836..12300)
                     /locus_tag="ENO07_07380"
     CDS             complement(11836..12300)
                     /locus_tag="ENO07_07380"
                     /inference="COORDINATES: protein motif:HMM:PF13380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDS01827.1"
                     /translation="MNTKELIEDFLQKKRIAFAGVSSSEKDFSRILMNDLVKRGYEIV
                     PVNPNIDEIGGMKSYASVKEIEPPVEAVLIMTKPEAAVNILQDSIEANIPLVWLYRGA
                     GKGSVSNEAIELAKEKDIRVIPGFCPYMFLPDSGLIHKIHGFFMKIAGSYPK"
     gene            12584..13837
                     /locus_tag="ENO07_07385"
     CDS             12584..13837
                     /locus_tag="ENO07_07385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDS01828.1"
                     /translation="MSRETTRSRIKEYRMDEKENNQTNNPETADLSALRINRLKEERI
                     KRRRWPKFIPWILVVAAMVIGYFIVLDRIKQGKKVDTTNVRLVKGASAQATLSASGYV
                     VAQRQSAVASKATGRLEYMAVEEGDLVEEGQIIARIENADVRAMLDLARANLKYARAD
                     SADAAAKYELNLKLFQAGSGTEEELQTYKARLDMAAATVEARRAQLRNAEIDLENTFV
                     TAPFTGKVITKMAEIGDIVAPMASSASSRGAVVVLADMSSLEVEADVSESNIQKVNVG
                     QPCEIVLDAYPEVRYRGSVKNIVPTANRASATIMTRIAFNQLDSLIYPEMSARIYFMS
                     PDEKPLEVEEPSFLGVSQNAVIEEDGEKYVYLVKGNVVEKKTVETGKTVGRTVEILAG
                     LEEGDEVVLTPPSSLKDGDKIEVGD"
     gene            13869..14543
                     /locus_tag="ENO07_07390"
     CDS             13869..14543
                     /locus_tag="ENO07_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDS01829.1"
                     /translation="MAESIVQTRNLYKEYIRDDMRIPVLYDINLDVPKGEFLALMGPS
                     GSGKTTLLNLIAGIDRPTRGDLSVAGESVAALTEGQLAHWRATHIGFIFQFYNLIPVL
                     TAFDNVELPLLLTKLSGYERRKHVEAALAIVGLADRMNHYPRQLSGGQEQRVAIARAI
                     VTDPSILLADEPTGDLDRQSAESIMTLMSRLNSEFNMTIIMVTHDPRAADAAKVVRHL
                     DKGELT"
     gene            14547..>14788
                     /locus_tag="ENO07_07395"
     CDS             14547..>14788
                     /locus_tag="ENO07_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDS01830.1"
                     /translation="MKFLNLIVKNAFRHKLRTILTILGLAITIMAFGIVRNILEVFSF
                     SESDLIPGHLITTHKTSQMQFLPYSHIEKMRQVEGV"
BASE COUNT         3955 a         3680 c         3538 g         3615 t
ORIGIN      
        1 aatcagcgaa aaccagagat acatgatccc gatcttctta tggtcgccgg tggcaagcca
       61 ttcatataat ttaccgcccg tgctcttccc gggctgctca gccggggccg gtttttcatc
      121 tgcagttaca gtagccataa tattccaatc atcttcctaa ttgataacat caactgtgtc
      181 ctcccgagcc tgctcaattg cagaggcggg ccggacctcg atattttccg cctcagcctg
      241 cttctctctt acccaggcca tgaattcatc ttccggcaaa accttgacat caattaacat
      301 ctcagcatga agcggaccac aaagctcggc acactgtcct tcatagacgc cgggtttggc
      361 cttgaaccag agttccgtga ttctgccggg cataaagtct tttttaactc caaaagccgg
      421 cacccaccag gaatgaataa cgtccatact ggtgccgtaa agagtaacat atttgtcggc
      481 cgggacaacc agtggttcct cggcaattgc tatatcgtat tcgggatagg tatatttcca
      541 gaaaaatctc tgggcttcca cctgcaccac cagatctgtg tggggcgagt aattgattct
      601 cttaatcagt gtaaaggtcg aaagtgccag aataacgagt gtaatcgccg gggctagggt
      661 ccaggctatt tccagcttga cattttcatg aaatgtggcc ggttctttgc cggggcgctt
      721 cctgaattta aaaatcacaa agatcagcag gccctcggca agaaatagaa agggcaggat
      781 cagcagcaaa gtgaaaatga aactgcctcg cacctcctct gccagcggcg tggcaggctc
      841 cgagagccag ttggttatgt cctcttccgc gctcagtgct gtaaaaagtc ccagtatcac
      901 cagcaaactc aggctgaaaa atattgcctg caataactta ctgaaagaca tctatctaaa
      961 cctctatctt gtcttcgata atctctgtaa ataattgtat atatcgttca gctcattctc
     1021 tgtcaagaat ccgaaattgt ggccgggaag tccgggtgta ccgttggtga tgaatttctt
     1081 aaatgtctct ttattcagaa atttaccgcc atccttgccg gccagaactc cagcgtccag
     1141 ataaataaca ctgctgggca caacttgttc cgcccgcaac tgtccctcgc cgtaacttcc
     1201 atgacaggag gcgcaatgtt tttcgaagag ctcgtttata ctgatgcccg acggagcttc
     1261 ggccgccaat gcgaactgtg ccagataggc ttgtctggcc gaatcgagcg gaatcgtctt
     1321 gggaactgcc gctgtgtcgg caactgcagt atcagcgggg gctgcctctc cctcatccaa
     1381 ttcgggaagt tcagccacca gttcaggggg attctggggc ggatcgggca cgaaggtctg
     1441 cacataatga gccattgcca tgcggtcctg ttcgggaagc aggtcaaaag caggcatgga
     1501 agttcccgtc acacctttgg taatggtatt ataaatttcc agagaggagg cgccgtattt
     1561 aaatttttcc tcctgaaaat tcctgggggg aggattgagg ccggctgatt tcgggccatc
     1621 accactaccg ttctggccat ggcatgaggc gcaattcagc tgatataact gcttaccttc
     1681 tttcagaagt tcttctgtgg gctcaagcag ggcgcgcagg ttgacttgtt caacttcttc
     1741 ctcgggcggt cctttctccc ggggcgggta atacttgcct tcaaactcgc cccgctcata
     1801 gccaatgaag aatgccttca gcaccagcac aatggccagc gccagcataa aaatatatag
     1861 aagcagattc aatttttcct tgatcttaga catgcagata cctgcctttt aaacatgtct
     1921 ccaggttggg atcgcccact gataccagtg aatgcctttt ataaaagcgg cggaccacaa
     1981 ggccgtagag cccgaagaaa ccgagaaaca taccgatctc tatccagctg atcatcctga
     2041 atgactcaga gaaactgggc acaaccatcc agtaaatatc cagccactgg ccgatcagaa
     2101 ctccaatcga aacccagaaa ataaccctgg gattggagcg gttcttttcc ggcatcaata
     2161 ctataaacgg aaggagccat tttaacaatg gcagtaacgc aaaaagatac tgccagccgt
     2221 tactcgagcg cggaatcagg taactaatct catgcggaag atcggcatac cagatcagca
     2281 tatactgcga gaatcctatg tacaccataa agaccgagaa ggccatgatc catgtgccca
     2341 ggtcatgcaa atgctttttt tcgacggcat tttgcaggta tcccctgttc ttgagggtta
     2401 tgaccacgat tgcgatcgcc gccatgccgc tcaagaataa tcccgagaag cagtaaactc
     2461 cgaagatagt ggaataaaag tgatcctgca gcgacatcag gagatccatg gacgagaacg
     2521 tgaatgtata tccaaacaat atcaggaaga atatgctcag ctttttgttg cggcgggtga
     2581 gccgtggatt ccgggtctta tcctgcttca atgatgtgtt tacgatcata aatccgaaga
     2641 aaatccagag tgcgaagaag aaggcttcac gcacaataaa ccacagcgtg gtcatataga
     2701 cctcttttgt ataggtaaaa taatcgtgcg gcgccgtata ggcccaggaa taaagctctt
     2761 ttactcccag aagaagtatc gcaaccgtta tggccaggcc gggaagataa gaaaacagtc
     2821 cctcgcctat tctcctgatc gtgacagacc aggccgcact ggaggcgtat tgcaccgccg
     2881 caaataccag cccgcccaga gcgatagaca ggaagaagaa aaattctata agataattgt
     2941 accaggccct ctgggcatcc acgaataatc cggcaatgaa tgaggccacc ccgaccagga
     3001 aaaagagggc cagcacgaca tcaaatcgcc cattgttatc gagtgggccg atatttttca
     3061 tttccggtat tccttcttca tgcatgatac aaaattacct tctattgctg ctgcgtcgcc
     3121 agctcctcac ccgaaggatt catggatttc tgtaatactc tcaaataatg gataatcgcc
     3181 cagcgggttg caggatcgat ataattccga tagctgggca tattaccctg gccataagtt
     3241 atcaggtgat atatcctgcc gtcggaccaa tctataactt tcctggaata caaaaccggc
     3301 ggctttgtca tcttgggaat aatcggacca tcaccggccc cggcttcacc atgacaggta
     3361 tagcagaaga tatcataata tttctggccc acagacagga cctcctctgt ggccgggatc
     3421 ggatttatca gttctgatgc cgccagcgta tctgttgcct ggagctgata gggctcgaaa
     3481 tttctcggaa ctgtgccctc tggcggcagc ctcatcgcta tctgaccgga atcggagggc
     3541 tctttctcct gcggcttgat ggccggggaa acatacatat ccggtgtgta ctgaagctct
     3601 gtggtctccc tgtctccaca ggagatcaac aaagccagaa ttccaaaaat caatatgcta
     3661 tatttcaaat tcatcgacat cttcaatacc atgtttcgta aaaaattccc tgatgtcttc
     3721 tatatttcca ttgtcctcga caggaatcac aaccgcgaat tgattatctg ttaatcgttc
     3781 acgaatcatc ttcggatcct tggcgggata gaaccggccc agatgaaaga gcagtataaa
     3841 ggttgtaatg ccggccagga gtacgccgca ttcaaagacg attggaatga aggcctgcca
     3901 tgagaaaagc ggtttgccgc cgacattgat aggccaggca acggccgagg tccatccggc
     3961 aaacaacagg cccaatcccg caccgatcac aagcatcagc ttcgccgccg ccggcacccg
     4021 ggattttttc aggcccagcg cctcctcaaa accatgcacc gggtatggca tcacaccatc
     4081 caggttcttg tagccatgct ccctggccct gctcccaact tcaagaaatt gttcgggatc
     4141 atccatgaat ccggctataa tcctgctctc aggcattggc tcccctcctg aatggcctgg
     4201 ttaaaatgct tttcaattca gcaattgaaa ccgccggaaa gaattttata aacagcaaaa
     4261 acagcgtgaa gaataatccg aacgagccca gtgtcactga ccagtcggcc caggttggtg
     4321 tgtacatccc ccagttcgat ggcaggaagt ccctgtgcaa tgatgttaca atgatattaa
     4381 aacgctcgaa ccacatgccg atattgacac agatcgaaac cgcgaacata atcggaatcg
     4441 aggtcctggc tttcttgaaa aagaacacca acggtataaa gacattacag atcagcatgg
     4501 tccagatcgc ccacgaatac ggtcccacca gcctgttcaa aaacacatac tgttcaaacg
     4561 gttcatcaga gtaccaggcc acgaagaact ccatcaggta ggaataaccg acaaacatgg
     4621 aggtcagcag tatgactttg ttcatcattt ccaggtgcct gatggttata tatttttcaa
     4681 gattcatgct ctttcgcagt attaccagaa gggtgaccac catccccatg ccggagaaaa
     4741 ttgcgccggc cacaaaatag ggcgggaaaa tcgtagcatg ccatcccggc agctgtgaaa
     4801 cagcgaagtc gaaggatacg atactgtgca ccgaaagcac cagaggcgtc gccagccagg
     4861 caaattgcag ataagctctc tcatagtgat tccagtccct ggccttcccc ttccagccca
     4921 tagccaaaat acgataaaac catttctgta ttttattttt agccctgtcg cgtagtgttg
     4981 cgaaatcagg caacaggccc tggtaccaga ataaaaatga tatagtcaaa tatgtcgtaa
     5041 cagcgaaaac gtcccagacc agaggagatc ggaagttgac ccagagttcg cgtgcattcg
     5101 gatacggtac cagccaatat gccacccagg tgcgcccgac atgtatcaat gggaataagc
     5161 ccgcgcatat gaccgcaaaa atcgtcatcg cctcggcggt acggttaatc gaattgcgcc
     5221 atttctgcct gagcaggaat aaaattgccg agatcagtgt gcctgcatgg ccgataccta
     5281 cccagaagac aaagtttgtg atatacacac cccaggccac cggatgattc aatcccagaa
     5341 taccgatacc cttcaacatc tgtatggtaa ggacaaaacc cagaagtccg accccggcta
     5401 atgccatcat aaagaggatg atccagataa ccccgggctt cctgattacc ggggccataa
     5461 catcatcagt gacatcactc atggttatgt cactggaaat ccatggcccg ccgtatttgc
     5521 ggccctcgat ctccaggggc tttatgtcag tgctcttcat cttcatgctt atcttttttc
     5581 gcatccttgt tccttaccag tgtcaggtag gaaacattcg gatcgatatt caactctacc
     5641 agagctttga aggctctggg ctgatgagat tctttcgaga caaggctgtc cttctgattc
     5701 agatcgccaa atgatatcgc acccgccgga caggtctgct ggcaggcggt ctggacctca
     5761 tccgggccaa tcggctgacc cctggacttt gcctgatgcc gcgcgtcacg aattctctgg
     5821 atacagaatg tgcatttttc catcacaccc ttgctgcgga cagtcacatc cggattcaaa
     5881 agcatgtgca ggggcttttc tttataggca ctctcgtaat aatcgtagta gttgaatctc
     5941 ctgactttgt aggggcagtt gtttgaacaa tacctggttc ccacgcagcg gttatatatc
     6001 tgcaaattaa ggccctcgtc attatgcacg gtagcaagaa ccgggcagac cgtctcgcag
     6061 ggagcatcat cgcagtgatg acatagcatg ggctgatgaa tagtatccgg attattctcc
     6121 tctcctgaat agtagcggtc gacacggatc cagtgcatct cacgcccgtt cagaacttcg
     6181 tttttgccaa ccaccggaat attgttctcg acctgacaag cggtcatgca agcgttgcag
     6241 ccgatgcatg aattcagatc tattaccata ccccatttat gaccgggata gcggtgatct
     6301 tttttccaga gtgtctgctg tgagccatgt ccctggtgcc ggccggcggt tggatcttct
     6361 ctatattctt ccagcgtggt ttcatagaga atgggacgat gttcgatata gttatgtccc
     6421 tggactgacg ccagcggaat acgcttaccg gatttcgtaa gctcaactcc ggttaaggtg
     6481 tagtggatat cagaatctat acttttcgcg agcttgaagg cattcacccc tgctttatta
     6541 ccgacatcgc cgaccgaggt cctgccccag ccaaccatga tgacagccga atctttatgt
     6601 gttccgggag caagatgcac cgggacatca agagatttgc cattgatagt caaggttaca
     6661 acgtctccct ctttcaggcc catttcatgg gcgcgtgagg gagcaatggc aaggtaatta
     6721 tcccaggcga tacgtgaaac cgggtcggga gtctccagga gccaggcgtt attcatcgaa
     6781 cgaccgtcaa tgtgaataga ggatgcgctc agaataagtt cgggcccatt ggaaaggcca
     6841 ttgactctcc cgagagccgc aacgggctca atccccaatt cggtcgcccc gaggcccccg
     6901 gttttataag atttcgattc aaagaagcca tgctgaagaa ctttaaccca gaaactctgg
     6961 aaatctccgg ccagtccggc atctctataa atattattct gccagtactt tttaacataa
     7021 tcatagaatt taatgggcgc ttcagcggat gagaaatcct gaacaccaag gccgaatccg
     7081 aaggcgataa gcgaatcctc gagctgacga ttattccaga gcggagttat ggtcggctgc
     7141 tggatagcat aaagcccggt gtatggctcc gaatctcccc agcattcggc gccatgcaat
     7201 ccgggcagga catattcgca taattcggcg gtctcattaa tcctgtcatc caatgatatt
     7261 ctcagcggga catgcttcaa tgcttcggca aatccgaaat catccggcag gctgtataga
     7321 ggatttgttc cagcaatcaa aatcgccgaa acccgaccgc tattcatatc atttacgagc
     7381 tgcctgagat cgccatagcc gctcaaaata tggctgtctg gaattgagtc cgtctcgata
     7441 gtgccgcttt cattgtccag aagagaattg agtacatgga ctagctggtg aagtgcctga
     7501 gaattctgtc cctgatcggc aaatgattcc gagagtatga tcgacctgcc gcggttattc
     7561 cagagatcat ccgagatctt ctttatagtc cccatgggga gcccgagctt ctcttcaatc
     7621 tcatagggat caaatttccg cacaatatca gcggagattc gctgggccag cccgggcaca
     7681 cgcttgtcca tataaatcaa ctgcaacata agagcgccgg ccagcaccgg caggtcggac
     7741 attttcaccg ggtagcgctc atcggcattc gagcccatct cggacattgc cggctcgaaa
     7801 gcatatactc tggacatatg attgtcctta atcttccttt tttcagcgaa atcacgcatg
     7861 tgccggacac ggtcacgccc tgaagcaaac aggtcggacc cgaggagaac caccacatca
     7921 gcattatcca gatgatactg cgggagctta ttcgatccga agccctgctg acgggagcgg
     7981 gcatagccgt catcaaaaag agcttcatat ttgtaatgcc tgccgttatc gaaaatattc
     8041 aggaatttat tgaccaggtc caatttggcg ggaccatgat atgcgcctgt cagaagcacc
     8101 acctgtccct cggcattttt cagccggcca acaatgtctt gatcagcatc agcgaactcg
     8161 attttctctc ttgatccatc ggcaacgatc ttcacaggat ttcgcaggcg tcccggatca
     8221 taaagatcca gaagcgatgc ctggcctcgt gcacacagcc gccccttgtt gacgggatgt
     8281 tccggattgc cctccatctt gatcggccgt ccttcacgtg ttttcaccac caatccacag
     8341 gcggccggac acgctgtgca ggtagaggca taatagttgg gaataccgag agtgatctcc
     8401 tccggctgac tgacataagg tacgattttc tcgactggtc gattgccgca ggctatcatg
     8461 gagaaaatcg ccgctgcacc ccccagcttc ataaaatctc tgcggctgaa tccctgggga
     8521 ccaaaatcct gcttgaacgc tttttcaggc ttattgaaaa attcctcccg gcgaagacgt
     8581 tttacctcgg gatcgtgata atattcctca agcgtggacc agtatctttt tttcttatca
     8641 gtcataatta ttccagatca attgtgacag atattacatt gaaccgaagc attatgatgc
     8701 gggaaaatcc cggtcgcggg ctcgacatca agggtatctt tctttagcgc ttcgataccc
     8761 ttcgcctcgg cgctttttaa tgtcagactg tcgatgtatt tcaagtatgg ataccagcga
     8821 acattattgg caatcgatgc cttatctccg ggcttcaatc tggctgcata ctgctgtgaa
     8881 taatcgctgt tgcgatgaca ttgaatgcag tcacccatct ccagccgatt cacctgtccg
     8941 atcacggcca tcccggccac atcgccatga caggtctcac attcaacacc ggcattgaca
     9001 tgccatttat ggttgaaata ggagtggtcc ggaagcctgt gcacccgcac ccattctatc
     9061 gattttcctt cgctgtaaat cctggtgagc tgctggatat tctctttgtc gattgccacc
     9121 accgaatgac agcccataca gttttccata gcagggattc cggcataggg acctttttcg
     9181 gcggcatggt ggcagtacag gcagtctatt ttattgtcgc ctgcatgaat tttgtgcgaa
     9241 aatggaatcg gctggtccgg cgtatagccg acattattat agacattggc ggcaaaattg
     9301 taacctatga ttagaaaaat aataacgatt aggataagca ggattctttt ctttatgaaa
     9361 tacgtaacct tcaaagcctg accctttata atctcagttc atcactgcgg gacattgatc
     9421 gatcagttta ttttaaattt caaagcacac caaaatgata attagctgaa taaaaaccgc
     9481 accacctcgt ggaaggtttc aaaaatttac agtttcatat caatgcacgg cccgggttcg
     9541 tgtcataatc gaattttccc ttccctgtac ttatttctgc ttgaaatgcc gctttggatc
     9601 cagataaaga gtcttcacct caattcagac ataattagtt agtttattta aacagagctt
     9661 acctgtcaat ataaatatgc cgatgtaccc ggcaattaac gaaattagac cggattgccc
     9721 acggaacaat tgacagatgt taatgttgat gttattttag cagattaatt tgataaaact
     9781 gattaggagg aagacgtatg tcccctgttt ctcaaacggt attggatgga ctgaatgaag
     9841 gcatcaaggc cgagctggcc gcttatgttt tttatatgaa gggaatgcat tccaccagcg
     9901 agcaaaggct gaaggacctt ctggcaacgc tcgcatccga agagaaggat cattacaaga
     9961 ttcttgaaag gcagtatgac aatctagtcc gttccgaaat ttgggcgcct tataacgata
    10021 taatgctgca gcccgggctt ccgaatattg acgagcaggt tgaggatgtt catgatgagt
    10081 atatcgacga aatcacagaa aaaacttcgc ccatgcgaat cctcgaaatt gctttgatgc
    10141 tcgagaagcg cgcccgtgac cactatgccg gcatggccga aaaggttgat gatcccaggg
    10201 gcaaggaaat gttcaaatac ctgtccaaat tcgagaacgt gcatgtcaat aagatcacga
    10261 agatgatgaa agagttcgaa aaatagtagt cctggtatct tgatttcaca agccggaggc
    10321 caactccggt tttttattag aatatagctc ttacccgata gccggaacag ccggaatcgg
    10381 cggtatgtta aatacttccg taagatccaa ataaaaagag gaaggcatat gagaactgaa
    10441 aaagattcca tgggcgaaat agaaattccg gatgatgccc tttacggtgc gcagactgcc
    10501 cgcgcagtcg agaatttcaa ggtttcggga cggacaggat atagggaatt tatttacgcc
    10561 acagttcaga taaaaaaggc tgccgcaatg gttcacaaag atctggggct gctggataag
    10621 gagcaggctg aggctatcgt agaagctgcc gatgagattc tcgccggcaa atacgatgag
    10681 cattttgttg tggatatctt ccaggctgga gcgggcacct cgcatcacat gaatgtcaac
    10741 gaggttctgt ccaacctggc caataaatca ctgggcggga aactggggaa atatgagcat
    10801 gttcatccaa atgaccatgt caatatggcc caatccacca atgacgttat cccgacagca
    10861 atcaggctgg cggcgcttca actcttgaaa gatttctatc cggcgctgga agagaccata
    10921 aaactcatgc agcataaagc caaagaattt gatgatataa taaaatccgc ccgcacacat
    10981 ctccaggatg cagttccgat tagattgggc caggaattcg ggggctacgc cgacaacctt
    11041 gaaaaacatc tccagagtat aaaaacagcc gcggtggatc tgaaaaagct aggaatcgga
    11101 gggtcggctg cgggaaccgg attaaacgtc catccaaaat accgtcagaa aatggctgaa
    11161 actctttcca ggctgataga tgcagatttg aaaatggcgg ataattattt cgaggcaatg
    11221 cagtccatgc ggccgtttgt taatctctcc ggcgcgctgc gcggcctggc ctctgatctg
    11281 gggagaatcg ccaacgattt caggctccta tcctcgggcc cacgtacagg tctgctggaa
    11341 ataaatcttc ctgctgttca gcccggatcc tcaattatgc ccggtaaagt caatcctgtc
    11401 ctggccgaga tgctcaacat ggtctgttac agagtgatcg gcaatgacct gacaatagct
    11461 aatgccgggg gcgcaggtca actcgatttg aatgtcatga tgccgcttat cgcggacacc
    11521 ctgatcgaat ctctgcggat attgacaggt ggtttaagat cgttcaacga gcgctgtttg
    11581 aagaatgtca ccgccaatcc ggaaagatgt aaagcttatg ccgagaattc gtttgctatg
    11641 gtgaccgcac tcaataccgt aatcgggtac ctgaaggcgg ctgaagtcgc gaaggaatcg
    11701 gctaaaagcg gaaagccgat caagcagatt gtgggcgaaa aggggtatct gaaagaagag
    11761 gagcttgaca agtatttatc gccggaaaag ctgactgagc cgggaattcc gggcaaggat
    11821 taatatcctc cgagactatt tgggatagct gccggcaatc ttcataaaga agccgtgaat
    11881 tttgtgaatc agcccgctat ctggcaaaaa catataggga cagaagccgg gtattacgcg
    11941 gatgtctttt tccttcgcca gctctatcgc ttcattactt acagagcctt tacccgctcc
    12001 tcggtatagc cacaccaatg gaatattagc ctcaatacta tcctgcaata tatttactgc
    12061 ggcttccggt tttgtcatta taagcacagc ctccacagga ggctctattt ccttcacgct
    12121 ggcataggat ttcatgccgc caatctcatc aatattagga ttgacaggca caatctcata
    12181 gccgcgcttg accaggtcat tcatcaaaat tcggctgaaa tccttctcac ttgaagatac
    12241 gccggcaaaa gcaatgcgct tcttctgtag aaaatcctca atcagttctt ttgtattcat
    12301 attaatttat gatataatat aggttcaaaa ggttcagcgc aagtcactat taatatatta
    12361 ctcctataag gctcatggcg acacgaatgt ttccttttcg taattatata tccatgaatg
    12421 agttatatgt agatgcaatg tggtatattt cacgataaca gagttttttg tgcaacatcc
    12481 cgaataaagc gccgtaagtg gtggtaaggt atataaaacc aaacagttat tggattgctg
    12541 attgacaaat tgaaataatt ccataaatat aggcgaataa aagatgagca gagaaaccac
    12601 ccgatcaagg attaaagaat acaggatgga cgaaaaagag aataaccaaa ctaacaatcc
    12661 tgaaactgct gatctttctg cactgaggat caacaggtta aaggaagaga gaataaagcg
    12721 ccgcagatgg cccaaattta taccctggat tctggtcgtg gctgcgatgg tcatcggata
    12781 tttcatcgtt ttggacagga tcaagcaggg caagaaagtg gacacaacaa acgtgcggct
    12841 ggtcaagggc gcctcggccc aggccactct ctcagcttcc ggctatgtcg tcgcgcagcg
    12901 tcagtctgcc gtagcctcaa aagcaacggg ccgtctggaa tatatggcgg tggaggaggg
    12961 cgacctggtg gaagaagggc aaataattgc cagaatcgag aatgcggatg tgcgcgccat
    13021 gcttgatctg gcccgcgcaa atttgaagta cgcccgagca gatagtgcgg atgccgccgc
    13081 taaatatgaa ttaaatctga agttattcca ggccggttca ggaaccgagg aggagctgca
    13141 gacttacaag gcgcgtttgg atatggccgc cgcgacagtc gaggcccgcc gggcacagtt
    13201 gagaaatgca gagatagatc ttgaaaatac atttgtgact gccccattta ccggaaaagt
    13261 cataaccaaa atggcggaaa tcggtgatat cgtagcgccc atggcctcct cggcgtcctc
    13321 gaggggagcg gtggttgtat tagcggatat gagttccctt gaagttgagg ctgatgtctc
    13381 cgagtcgaat atacagaagg tcaatgtcgg gcagccctgc gaaattgtgc ttgatgccta
    13441 ccctgaggtg cgctacaggg gctctgtaaa gaacattgtt cccacggcca accgggcttc
    13501 ggctacgatt atgacccgca ttgcctttaa ccagttagac agcctgattt atcccgagat
    13561 gtctgcacgg atttacttca tgagcccgga tgaaaaacct ctcgaggtcg aggagccgtc
    13621 attcctggga gtctcgcaga atgctgttat cgaagaagac ggtgagaaat acgtttacct
    13681 ggtcaagggg aatgtggtag agaaaaaaac ggtcgaaacc ggtaagactg tcggacgcac
    13741 agtcgagatt ctcgcggggc tggaggaagg ggacgaggtt gtattgaccc ctcccagcag
    13801 ccttaaggac ggcgacaaga tagaagttgg tgattaagag aaaaaatatt tgaagataga
    13861 gaagtagtat ggcagaatct atagtacaaa caagaaatct ttacaaagaa tatatcaggg
    13921 atgacatgcg catcccggtt ctttatgata ttaaccttga cgtgcccaag ggggagtttc
    13981 tggccctcat gggtccttcc ggatcgggca agaccaccct cctgaacctg atagcaggca
    14041 tcgaccgtcc taccaggggc gatctttcgg ttgcgggtga gtcagtcgcg gccttgacgg
    14101 agggccagct ggcccattgg cgtgcgacgc atatcgggtt catattccag ttttacaatc
    14161 tgatacctgt tctgaccgcc tttgacaacg tggaattgcc gctgcttttg accaagctca
    14221 gcggctatga acgccgcaag catgtggaag cagcgcttgc aatcgtagga ctggcggaca
    14281 ggatgaacca ctatccgagg cagctttcgg gcggccagga acagcgggtg gcgattgccc
    14341 gggcaatagt aaccgatcct tcgatccttc tggccgacga gcccaccggt gatctggacc
    14401 gtcaatcggc ggagtcaatc atgaccctga tgagccgcct gaatagtgaa ttcaatatga
    14461 ccatcattat ggtcactcat gatccccgtg cggcggatgc agcaaaagtt gtcaggcatc
    14521 tcgataaagg ggagttgact tagatcatga aattcctaaa tctgatagtt aagaatgcct
    14581 ttcgccataa gctccgcacc attcttacca tactggggct ggcaatcacc atcatggcat
    14641 tcggcatcgt acgtaatatc ctggaggttt tcagtttctc tgaaagcgac ctgataccgg
    14701 gtcacctgat cacgacgcat aaaacctcgc agatgcagtt tctgccgtat tcccacattg
    14761 aaaaaatgag gcaggtggag ggtgtcaa
//
LOCUS       DSEB01000405            1506 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1018935, whole genome shotgun sequence.
ACCESSION   DSEB01000405 DSEB01000000
VERSION     DSEB01000405.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1018935"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            257..718
                     /locus_tag="ENO07_07400"
     CDS             257..718
                     /locus_tag="ENO07_07400"
                     /inference="COORDINATES: protein motif:HMM:PF11578.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 family protein"
                     /protein_id="HDS01831.1"
                     /translation="METVAVDRQCLFEEELSLTGITEYGFSWDDIRSGDKAIPLEGAR
                     FDINFEGLAKGQNINGKIIGTDYLNIRADGRFLLTIHGRITTDDGVNIAFYEDGILTP
                     SEDDPGKAGLRLNMRLNTADPRYSWVNKLQVWCVGTADTENRKVKVRAYSE"
BASE COUNT          418 a          330 c          371 g          387 t
ORIGIN      
        1 cgatgaacgc cggattgtga atgccggatc gattgcattg gtaccgacaa tggttcctca
       61 tgacctcaag aatgtgggcc aggggaaagt aagagtggct ggaattttcg gccgcaataa
      121 cataaccgcg acattcgaca tcccctgggc cgagctcaat tctaatgtgg ttagcactgc
      181 cgagatgaca aagcagactg cccaggcata agctgggagg ttacacaaat gtatcgataa
      241 gaaaggaagt gaaattatgg aaacagtagc agtagacagg cagtgtctct tcgaggaaga
      301 actgagcctc acgggtatta ccgaatacgg atttagctgg gatgatatac gctccggaga
      361 caaagccata ccgcttgagg gcgcaaggtt cgatatcaat ttcgagggtt tggccaaagg
      421 tcagaatatc aatggcaaga taatcgggac cgattatttg aatatccgtg cagacggacg
      481 ctttttgctg actattcacg gccgaattac tacagatgat ggggtgaata ttgcctttta
      541 tgaagacggc attcttacac cttccgagga tgatccgggg aaggcaggcc tgcgtttaaa
      601 catgagactg aatacggcag atcccagata ttcctgggtc aataagcttc aggtatggtg
      661 tgtgggcaca gcagacactg agaatcgcaa agtcaaagtt agggcatact cggaatagat
      721 gatttgtccc gactgattga aataaatggg atggactcag aatctatccc gtttattttt
      781 ttagggaatt atggattaag aagttgtcgc tgcctgatat gtctcctcga gtgtctccag
      841 catcgccttg acatctgtgg tcggcaggct gcaggtgaaa ttctggcaga cataggcggt
      901 ggccttctca ccgattgact tctgttccct ggtatactta gcatacttga caatttccgg
      961 ggaatcatcc atgccccgac ggaagattat gacccggtta ggaatatatt tctgccttaa
     1021 tgccatgatc atctcatggg tatccgactt ttcaagattg ccggagatca ctatctcaaa
     1081 tgacggtcca accatataat caacgacagt caaaagcatt gtgttgccag ccggatagcg
     1141 ctcgacctgg ttggcaaaag ctctcgtgag ctggcgggat atctcctccc actctgtttt
     1201 ggcagtaatg cgtgccagtc tgatcaggtt caatgctgat acagaattgc cggagggtat
     1261 cgccccatca tagatttctt tagacctgtg aatcaattgc tccgactcat cagaagtaaa
     1321 ataaaatccg ccatcatgaa aatcccagaa atgctctata agatattcct gcagttttat
     1381 cgcttcatca agaaaccgga tttcaaatgt ggcctcatag agttccagaa ggccccagat
     1441 cataaaggca taatcatcaa taaacccggt ctgattcgat ttttccttgc ggtacaaatg
     1501 taagag
//
LOCUS       DSEB01000406            3122 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003787, whole genome shotgun sequence.
ACCESSION   DSEB01000406 DSEB01000000
VERSION     DSEB01000406.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003787"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(396..>3122)
                     /locus_tag="ENO07_07405"
     CDS             complement(396..>3122)
                     /locus_tag="ENO07_07405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HDS01832.1"
                     /translation="VTVSDDCDPSPVVTHVGDVSDNNSCPETITRTYRATDASGNYAE
                     CAQIITIDDTMAPVITCPADITVVCNNPSDPSNTGTATATDNCDQDIEITYTDSQTGN
                     IITRTWTAVDDCGNSAQCDQLITLVPNSPPVCNVPNDTTIHQCNGDAICLPVSATDPD
                     GNLENCQITSGPGTLQNGEWCYTTTGSGAVTVTITCTDSCGEFCEESFTVTFTVNNAP
                     VLTVAADIDTFMCDASDQICFDNSASDADLPQDELHFEMPSAPAGAAIDQQTGQVCFV
                     PGSSGTYSFTIRLYDGCDAYDEGTVTVMVTENQPPTISANQEEFFFAYCPDADAEEKC
                     FNGFTVSDPDHSLSQLTISKIQGPGTFNPGTGSTCFTPDVMDSLYEFIYQVSDPCGAV
                     DYDTIDVNVVVLDNCDSITCIDVALGDTACVYNSTYIYAPITISNYVPSIGGFELLIT
                     YDPSAFTFVGAVQGDAIAEWEYFTYRTGPFGNCGNNCPSGMILLVGIADVNNGAPHPS
                     PSAYDPNGVLANLKFYVTDDRNFGGQSYPLRFFWMDCGNNMFSNEMGDTVYVDKRIYD
                     PFGGIRWDEFDDVNFPESERPFGTGAPDVCVIGDKTVPIRCVQFFDGYISICHPDSID
                     LRGDMNMNEIPNEIGDAVLYTNYFIYGPGVFIINLYGQIAASDINADGKTLTVGDLVY
                     LIRIMSGDAVAIPKLAPFSDETRVTLCTQNEGYTVTTNTSSDMAAILLSFAVDNQSSD
                     IAVKLADNLGQFSIMSDIVNDRLNVLIYSFDRIRIPAGKNEIINIEAADALKLISAEA
                     SDYNGNQLDVILNKVEVPTEFALSQNYPNPFNPETEIMLSLPESGNWYLDIYNIRGQL
                     VRQFSGYSGAGTVHIKWDSRDDRGNEAASGIYFYKARVDDRFSQTRKMILLK"
BASE COUNT          788 a          775 c          762 g          797 t
ORIGIN      
        1 ggattggcca gccagaactc gggtaagtca gtctcgaata ttttttcgat ctgggccggg
       61 gttgcctcga tccgatcacg gaaaatatct tcattgataa cctcagtccc gatgatcttg
      121 taggttcttt ctaaaccttt ttcggttttc atttgcgcct ccgtgcaata gcagggcgtt
      181 cctgtcctcc gccgaatcga ttatgaatat tagaattcac cggaggatct atgtctaaat
      241 aattataccc aatatttgag atcagacaag taaaaaaatt cttttccgac aggcctggcc
      301 ctcaaaagat atacgctgat cgatgcggca aataggtgca aaaaaaatgc cccgtctcaa
      361 gcatgacggg gcatttctgc aaaaagcatt tttattcatt tcagcaggat catcttgcga
      421 gtctggctga acctgtcatc aacacgggcc ttgtagaagt aaattccgga ggcagcttca
      481 ttgcctctat catcgcggga atcccattta atatgcacgg ttccagcacc tgaataacca
      541 ctgaactgcc tcaccaattg accccggatg ttataaatat ccaggtacca gttccccgat
      601 tcaggcagcg aaagcatgat ttccgtctcc ggattaaaag gattgggata attctggctg
      661 agggcaaact cggtgggaac ttccacttta ttcaggatca catccagctg attaccattg
      721 taatccgagg cctcggctga gatcagcttg agcgcgtcag cagcttcaat attgattatc
      781 tcgtttttgc cagccggtat tctgattcta tcgaaactgt aaatgaggac gttcagcctg
      841 tcattaacta tatccgacat aatcgagaac tgcccgagat tgtcagcaag tttaaccgca
      901 atatcgctac tctgattgtc caccgcaaac gagagcagaa tcgccgccat atctgaggat
      961 gtgttggtgg tgacggtgta gccttcgttc tgcgtgcata atgttactct ggtttcgtcg
     1021 gagaacggag caagcttcgg tatagctacc gcatcaccgc tcattatccg gatcaggtaa
     1081 acaagatcgc ctacggttaa ggtcttaccg tccgcattaa tgtcggatgc ggctatctgg
     1141 ccataaagat tgattatgaa gacgccgggg ccataaatga agtagttggt gtagagcacg
     1201 gcatccccta tttcattggg aatctcgttc atattcatat caccgcgcag atcgattgag
     1261 tcgggatgac agatgctgat ataaccgtcg aagaactgta cacagcgtat tggaacggtc
     1321 ttgtcgccga tcacgcatac atcaggcgct ccggtaccga atggcctttc gctttcaggg
     1381 aagttaacat cgtcgaactc gtcccacctg attccgccga agggatcata aatgcgcttg
     1441 tcaacataga ccgtatctcc catttcattg gagaacatat tattaccgca atccatccag
     1501 aagaagcgca ggggatagga ctgaccgcca aagtttcgat cgtcggttac ataaaatttc
     1561 agatttgcca gcacaccatt gggatcgtat gcggagggcg atggatgtgg tgcgccgtta
     1621 ttcacatccg cgatgcccac cagcaggatc ataccgctgg gacagttgtt tccgcagttg
     1681 ccgaacggcc cggttctgta ggtgaaatac tcccactcag cgatggcatc accctgaacc
     1741 gcgcccacaa atgtgaaggc cgagggatca taggttatca gcagttcgaa accgcctatc
     1801 gaaggaacat aattgctgat tgtaatcggc gcatatatat atgtgctgtt atatacgcag
     1861 gctgtatcgc ccagggcaac atcgatacag gttattgaat cacagttgtc caagactacg
     1921 acgttcacat ctatcgtgtc ataatcaacc gcaccgcatg gatcgctcac ctggtagatg
     1981 aattcataca gggaatccat cacatccggg gtaaagcaag tactgccagt gcccggatta
     2041 aacgtgcccg gtccctgaat ttttgagata gtcaactggc tcagggaatg atcggggtcg
     2101 ctgactgtaa agccattgaa acatttctct tccgcatcgg catcgggaca atatgcaaag
     2161 aagaattctt cctgattagc cgaaatggtc ggaggctggt tctcagtcac catcacagtg
     2221 accgtaccct catcataagc atcgcagcca tcatataacc tgatagtgaa gctatatgtc
     2281 ccggagctgc cgggaacgaa gcaaacctgg ccggtctgct ggtcgatggc ggctcctgcc
     2341 ggcgctgagg gcatctcgaa gtgcagctca tcctgcggca gatcagcatc gctggctgaa
     2401 ttgtcgaagc agatctgatc acttgcatcg cacataaatg tatcgatgtc tgctgcaacc
     2461 gtaaggacag gcgcattgtt aaccgtaaat gttaccgtaa atgactcctc acagaattca
     2521 ccgcaggaat cagtgcaggt tattgtgact gtaacggctc cggaaccggt cgtggtataa
     2581 caccattcgc cattctgcag ggtgcccggg ccggaggtaa tctggcagtt ctcaaggttg
     2641 ccatccgggt cagttgcaga aaccggcagg caaatcgcat cgccgttaca ttgatgaatt
     2701 gtcgtgtcat ttggcacatt gcaaaccggc gggctgttcg ggacaagagt gatgagctgg
     2761 tcacactgtg cagaattgcc gcaatcatcg actgcggtcc aggtacgcgt gataatattg
     2821 ccggtctgcg aatctgtgta agttatctca atatcctggt cgcagttatc tgttgccgtc
     2881 gccgtaccgg tatttgacgg gtcggaggga ttgttgcata cgactgtgat atcagccgga
     2941 caggttatca ccggtgccat ggtatcatct atagttataa tctgtgcaca ttcggcataa
     3001 ttacccgaag catccgtggc ccggtaggtc ctggtaattg tctccggaca gctgttattg
     3061 tcagatacat ccccaacatg cgttactaca ggactcggat cgcagtcatc tgacactgtc
     3121 ac
//
LOCUS       DSEB01000407            1983 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1010289, whole genome shotgun sequence.
ACCESSION   DSEB01000407 DSEB01000000
VERSION     DSEB01000407.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1010289"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..439
                     /locus_tag="ENO07_07410"
     CDS             <1..439
                     /locus_tag="ENO07_07410"
                     /inference="COORDINATES: protein motif:HMM:PF00842.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDS01833.1"
                     /translation="YLSYRLAGGENNLLTPALTWKTKVAQIKNVPKGSFIGYGCTYRT
                     TADSKIAVLPLGYYDGYDRKLSNLGYVLIKGERAQVRGRICMDNIMVDVTNIPNVKIE
                     DEVVLLGRQDGDVITAEQMASWAQTINYEIVARINPLLPRKII"
     gene            449..1225
                     /locus_tag="ENO07_07415"
     CDS             449..1225
                     /locus_tag="ENO07_07415"
                     /inference="COORDINATES: protein motif:HMM:PF12697.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDS01834.1"
                     /translation="MPSIDSKGVKIYYEDTGSGTPIVFVHGLGIDRRIFKYQIDALKE
                     KFRLITFDLRGHGKSEAPETGYSYNYFADDLKNLIEHLALKDFHLVGLSLGGAVAVRY
                     QIENPEKAKSLTLIGSHIVGYMKFQDWPNMFKIARKEGVEKARETWKNFRLFENLKDA
                     KEKFDILTDMVNDFSCAYWLDPDPRYDEPNDLKRLDQIEIPVLLAAGKDDQDFYPIAE
                     LARVELPNSKFIEFDSGHLVSFEKPDEFNNVLLKFIEEID"
BASE COUNT          540 a          451 c          459 g          533 t
ORIGIN      
        1 ctatctttcc tatcgtttgg ccggcggcga gaataatctg cttacacccg ctctcacctg
       61 gaagacaaaa gttgcgcaga tcaaaaatgt gcccaagggg tcgttcatcg gctacggctg
      121 tacataccgc acgaccgctg attcaaaaat cgccgtactg cctctgggtt attatgatgg
      181 ttatgaccgc aaactttcga atcttggtta tgtcctgatc aagggcgagc gcgctcaggt
      241 acgcggcaga atctgcatgg ataatattat ggtggatgtt accaatatac cgaatgtgaa
      301 gatcgaagat gaggtcgtgc ttctgggccg tcaggatggc gatgttatca cggcagagca
      361 gatggcctcg tgggcgcaga ccatcaacta cgaaattgtg gcgagaatca atccgctact
      421 tcccagaaaa attatatagg aggtcgctat gccttccatc gattcaaaag gggtcaaaat
      481 atattatgag gatactggtt cgggaacgcc aattgtattc gtgcatggtc tcggtatcga
      541 cagacgaatt ttcaaatacc agatcgatgc tctaaaagag aaatttcgcc tcataacatt
      601 tgatctccgc ggccatggca agtcagaagc tcctgaaacc ggctactcct acaattattt
      661 tgccgatgac ctgaagaacc ttatcgagca tcttgccctg aaagattttc atctggttgg
      721 actctctctg ggcggagcgg tagcagtccg ttatcagatc gagaatccgg aaaaagccaa
      781 atcgttaaca ttgatcggct cgcatatagt cggctatatg aaatttcagg actggccaaa
      841 tatgttcaag atcgcccgca aagagggtgt ggagaaggct cgcgaaacat ggaagaattt
      901 caggctgttt gagaatctta aagacgccaa agagaaattc gatatcctca ccgatatggt
      961 caatgatttc tcctgtgcat actggcttga tcccgatccc cgctacgatg aaccgaatga
     1021 tttgaagcgc ctggatcaaa tcgagattcc tgtgcttctt gcagccggca aggatgatca
     1081 ggatttctat ccgattgcag aactggcccg ggtagaactt cccaactcaa agttcataga
     1141 attcgattcc gggcatttag tatcattcga aaagccggac gagttcaata atgtcctttt
     1201 gaaatttatc gaagaaattg actagttaaa atcgactgaa tgcactctac tggttccctc
     1261 agatcctgat tcgcccttgg gcgaattgtg atctgatggt atatccatct ataataaagt
     1321 gtcagttctc atccccgatt ccatcgggga caggaactga cacaacttat accaaagcat
     1381 tgctttgtcg aaaatctcaa agactgatac gcattccgtc ataagcaaac tccatcggaa
     1441 agtactgata ttttatcgcc ggcagggaac gcagattgcg gtaggacatt ccaaagtcct
     1501 cttcgatatg agtgagcagg attttatgag cgcgcatctg ctcagcagtc tcaagcatgc
     1561 tttcgaaggt gatagtgctt gcggtgatct gtttgtttac gatctccctt ctgaagacag
     1621 ttttgtcctc ccagtaatag cagggcacaa tgataaggtc cgcatcgaaa gcctcgctga
     1681 tcggcttgaa ttgatttgac cttgcaggca ggtacacgat cttatgggcg ccgtcataaa
     1741 taacaaaata ataaccgtca tctgcatcga gtctcaccgg caaaatgtta atattgccga
     1801 tgcccataat ttcgcagtca tgcatctcga tcaggttgat gatacagaag tcattgcggt
     1861 attttttcaa cttttgatag atgtaatttg tctcgtcctt cagagccggg atatagacat
     1921 tgatggcctc ccagatcgga ttgtcaatca ccgaatcgta attcaggttt tcgattacac
     1981 gca
//
LOCUS       DSEB01000408            3525 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002876, whole genome shotgun sequence.
ACCESSION   DSEB01000408 DSEB01000000
VERSION     DSEB01000408.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002876"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            2..367
                     /locus_tag="ENO07_07420"
     CDS             2..367
                     /locus_tag="ENO07_07420"
                     /inference="COORDINATES: protein motif:HMM:PF01593.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01835.1"
                     /translation="MWTSVIFPEQAPDGYALFRTMMGGAKNEEIVKESKESLARIARK
                     ELAPILGLKGEPDYQKVIIWERAIPQYVIGHRKRLERINRRLKEIGNIHLAGNAFTGI
                     GLNDTIKRSYNIVESIRID"
     gene            364..1260
                     /locus_tag="ENO07_07425"
     CDS             364..1260
                     /locus_tag="ENO07_07425"
                     /inference="COORDINATES: protein motif:HMM:PF00149.26"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01836.1"
                     /translation="MIIYSNIQEGFDLNCIFVSDLHGLSERYIKLFKLIAHEKPDAVF
                     MGGDILPSGMFMNYETHAIGGDFIDDFLLVRFAGLKAALEDKYPRVFIILGNDDGRNR
                     EIDFISGTEAGIWEYLHFNKAELGKYKVYGYSFVPPTPFRLKDWERYDISRYVDPGCI
                     SPEEGRYTVAVPQREKRYSTIKDDLDEYTRDDDLENAIMLFHTPPYQTKLDRANLDGK
                     MVDHVPLDVHVGSIAVRRFIESRQPLLTLHGHIHESASITGTWKDKIERTHMFSAAHD
                     GRELAVVRFNPEDLENAVREII"
     gene            complement(1253..>3525)
                     /locus_tag="ENO07_07430"
     CDS             complement(1253..>3525)
                     /locus_tag="ENO07_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDS01837.1"
                     /translation="FMSDQLPFINIAKNFVEVGDFYHLLQNVIFSAESHGKLGGKSAG
                     LYLAAQILKKSSEYADILGTIKIPKTWYITSDMLLHFMHFNNFDEIVEQKYKDINQVR
                     FEYPHIVQTFKNSHFPPEMVKGLSLALDDLGDRPLIVRSSSLLEDRTGAAFSGKYKSL
                     FIANQGSKRERLDALLDAIAEVYASTFSPDPIEYRTERGLIDFGEEMGIMLQEVVGTQ
                     VGHYYLPTFAGVAFSKNEFRWSARIKREDGLLRLVPGLGTRAVDRLADDYPILIAPGQ
                     PNLRVNATVDETVKYSPRWIDVINLETNQFETVEVDYLLREYGAEIPGINKLVSIHED
                     GTLRQPMGSNFDFEREQPVVTFEGMISSGRFIQQMQTILRVLEEKLETPVDIEFASDG
                     KDFYLLQCRPQSYSEQSQPAPIPKDVAEEKILFTANKYISNGIVTDITHIVYIDPENY
                     GELAEKADMLAIGKAVGKLNKLLPKRQFILIGPGRWGSRGDIRLGVSVTYSDINNSAV
                     IIEMARLKGNYVPDLSFGTHFFQDLVEAGIRYIPLYPDDDGIIFNERFFRSAPNILAD
                     VLPSFAGLKDVIRLIDIPRAADGQIMKILMNAELDEAMGLLTRQTVREKKGGADLEMP
                     KTKISKYWQWRMNIADLIAAKLDPERFGVKALYIFGSTKNATAGPGSDIDLIVHVNGT
                     PEQRRLLDTWFEGWNHVIAEMNYQRTGYRTDEILDVHYVTDEDIKNRTSYAVKIGAVT
                     DAARPLKIGKSANPSK"
BASE COUNT          876 a          864 c          894 g          891 t
ORIGIN      
        1 gatctggacc tcggtgatat tcccggaaca ggctccggat ggatatgccc tttttcgtac
       61 aatgatgggt ggtgcgaaaa acgaagagat tgtaaaagag agcaaggaga gtctcgcccg
      121 aatagcaagg aaagagcttg caccgattct cgggctcaaa ggcgaaccgg attatcaaaa
      181 ggtgatcatc tgggagcgag ccattccaca gtacgttatc ggacacagaa aacgcctcga
      241 aagaataaat cggcggctga aagaaatcgg gaatattcat ctggccggca acgctttcac
      301 aggtatcggg cttaacgaca ctataaagcg ttcgtataat atcgttgaat caattagaat
      361 cgactgatta tatattcaaa tattcaagag ggttttgatt tgaactgtat ttttgtttcc
      421 gatcttcatg gcctctcgga aagatatatt aagctcttca aactgatcgc gcatgagaaa
      481 cctgacgcgg tattcatggg aggcgatata ctgccgtcgg gcatgttcat gaattacgag
      541 acccatgcaa tcggcggtga ttttatcgat gactttctgc tggtcagatt cgcagggctg
      601 aaggctgcac tggaggacaa atacccgcgt gtttttataa ttctgggaaa tgatgacgga
      661 cgtaatcgag agatagattt tataagcggc accgaagcag gcatctggga atatctgcat
      721 ttcaataaag cagagctcgg aaaatacaaa gtctatggct acagtttcgt cccgcccacg
      781 ccgtttcgcc tgaaggactg ggagaggtac gatatttccc gctatgtcga tccgggctgt
      841 atctcgcctg aggagggcag atatacggtc gctgttcccc agcgggaaaa gcgttactcc
      901 accattaagg atgatctgga cgaatatacc cgggatgatg acctggaaaa tgctataatg
      961 ttattccata ccccgcccta ccagaccaaa cttgaccggg cgaatctgga cggcaagatg
     1021 gttgaccatg ttcctcttga tgtccatgtc ggcagtattg ctgtgcgtag atttatcgaa
     1081 agccgtcaac cattgttgac tttgcatgga catatacatg agtcagcttc cataaccgga
     1141 acatggaaag acaaaattga gcgtacccat atgttcagtg cagcgcatga tggacgtgag
     1201 cttgcggttg tgaggttcaa tccggaagat cttgaaaatg cagtcaggga gattatttag
     1261 atggattcgc tgatttaccg atcttaaggg gcctggcggc atcagtgaca gcgccaattt
     1321 tcaccgcata acttgtacgg ttctttatat cctcatcggt aacatagtgc acatccaaaa
     1381 tttcgtcggt cctgtagccg gtgcgctgat aattcatttc tgcgatgaca tggttccagc
     1441 cctcgaacca tgtgtccaga agacggcgct gttcaggagt gccgttgacg tggacgatca
     1501 agtcaatatc actgcccgga ccagcagtcg cattttttgt gctgccgaaa atgtatagcg
     1561 ctttaacacc aaagcgttcg gggtcgagct tggcggcaat caggtcggca atattcatgc
     1621 gccactgcca gtatttgctg attttggttt tgggcatctc cagatccgcg ccgccttttt
     1681 tctcgcgcac agtctgccgt gttaataagc ccatggcctc atccagctcg gcgttcatca
     1741 gaattttcat aatctggccg tcagccgccc tgggaatatc aatcaggcgg ataacgtcct
     1801 tcagcccggc aaacgacggc agtacgtcag ccagaatgtt gggagcgctc ctgaaaaatc
     1861 gctcgttaaa aataatccca tcatcatcag gataaagcgg gatatagcgg atgccggctt
     1921 cgaccaggtc ctggaagaag tgcgttccga atgacaggtc cgggacataa ttgcctttga
     1981 gacgcgccat ttctattatg actgcgctgt tgttaatatc tgagtaggtt acgctgacgc
     2041 ccaggcgtat gtcgccgcgg ctgccccatc ttcccggtcc gataagaata aactggcgct
     2101 tgggaagcag cttgttgagt ttaccgaccg cttttccaat cgcgagcata tcagctttct
     2161 cggccagctc gccgtaattt tcaggatcga tatagactat atgagtaatg tcggtcacga
     2221 taccgttgga aatatatttg ttggcagtaa agaggatttt ttcttcggcc acgtccttgg
     2281 gaatcggggc cggctggctc tgttcggaat aactttgtgg acggcactgc aaaagataaa
     2341 aatccttgcc gtcggacgca aactcgatat ccaccggggt ttccagcttc tcctcgagca
     2401 ccctcagtat cgtttgcatc tgctggataa aacggccgct ggaaatcata ccctcgaaag
     2461 tcaccaccgg ctgttcccgt tcgaaatcga agttgctgcc catgggctgg cggagagtgc
     2521 cgtcctcatg gatcgaaacg agcttgttga ttcccggaat ttccgcacca tattcacgca
     2581 gaagataatc cacttccacg gtctcgaatt gattggtttc gaggttgata acgtcaatcc
     2641 acctggggga atacttcacg gtttcgtcga cggtggcatt gacgcgcaga ttcggctgac
     2701 cgggtgcgat gaggatgggg taatcgtcgg ccaggcgatc taccgcgcga gtacccaggc
     2761 cgggaaccag cctcagcaga ccatcctcgc gcttgatccg ggccgaccat ctgaattcat
     2821 tcttgctgaa ggccactcct gcaaaggtgg gcagataata atgaccaacc tgggtgccca
     2881 caacctcctg cagcatgatc cccatctcct cgccgaaatc aatcaaacct cgttcggtac
     2941 ggtattcgat cgggtcgggc gagaaggtgg aggcatagac ctccgcaatc gcatccagaa
     3001 gagcgtccag cctctcccgc ttactgccct ggttggcgat gaagagactc ttgtacttgc
     3061 cggagaaagc cgccccggtt ctgtcttcca gaaggctgga gcttctgacg atcaggggtc
     3121 tatcgcccag gtcatcgagc gccagcgaca gtccttttac catctcgggc gggaaatgcg
     3181 aatttttgaa ggtctggact atgtgcgggt attcaaagcg aacctggttt atatccttat
     3241 atttctgttc cacgatttcg tcaaaattat tgaaatgcat gaaatgcagg agcatatccg
     3301 aagttatgta ccaggtcttt ggaatcttta tcgtgcccaa aatatctgca tactcgctcg
     3361 actttttcag tatctgcgcg gccaggtaca atcctgcgga tttgcccccc agcttgccgt
     3421 gactttcggc cgagaaaatc acattttgaa ggagatgata aaaatctccc acctcgacaa
     3481 aattcttggc tatatttata aagggcaatt gatcagacat aaacc
//
LOCUS       DSEB01000409            3888 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002306, whole genome shotgun sequence.
ACCESSION   DSEB01000409 DSEB01000000
VERSION     DSEB01000409.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002306"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(42..1979)
                     /gene="gatE"
                     /locus_tag="ENO07_07435"
     CDS             complement(42..1979)
                     /gene="gatE"
                     /locus_tag="ENO07_07435"
                     /inference="COORDINATES: protein motif:HMM:PRK04028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HDS01838.1"
                     /translation="MNWDNLDDSRFKELGFKCGLEVHQQLDTAKKLFCRCPVGYVDGP
                     PDGLIVRHMRPTLSELGEYDGTALMEFKTKKEVVYELFRDNVCTYEMDDTPPFPINQE
                     ALDIALQISMLFNCHIVDELHITRKQYLDGSIPTGFQRTAIVGVEGWIPYKDRKIRII
                     QVALEEDACREVHDIGHQIRFRTDRLSTPLVEIVTYPDMQTPTEAMEVDEVIGRVLRS
                     SGRVRRGIGVTRQDVNVSIDGGTRVELKGIDKTGYIKRLTEVEGLRQKSLLEIKSELR
                     KRGISPSTIHSEKKDLTEIFKKIAHPVISEVINNGGKVGGIMLERFAGILNYKTQPNK
                     TFADEISGRIKVIACIDKYPNIIHSDDISCAELPDEICDEIKKVFHMRHTDAIIITWG
                     TEADVNMALSEVKIRAVEATEGVPNETRQHIGEGITDFERILPGPDRMYPDTDSSPVA
                     ITKDRLDKLRAVLPDPAYSRDERWMELGLSDELVYSLGRSKFALMYDRLLKRNLTDPV
                     FLAYLFEGYLKNLKRQGRDLIFISDDLLAYILEQTGQRELPNEAVYVALDYLSSNGTN
                     IPLERLFDELNLIPMTESDVSSLIESKAAELDGDRFKSEEKLIEYIIGQVKSETRGKF
                     PPEKLKDMVKEQVKAKHPATS"
     gene            2099..3811
                     /locus_tag="ENO07_07440"
     CDS             2099..3811
                     /locus_tag="ENO07_07440"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HDS01839.1"
                     /translation="MKRKFRIALAQINSTVGALDQNLDKIISYARRAEEHGCDIVAYP
                     ELALTGYPPEDLLLKKAFNLDVSRTLKKLIKEKIRPIMVFGYPDFDGGKYNSAAVIYN
                     NKLVASVNKFMLPNYGVFDEKRYFTPGNGTYIFDLGFMRFGVNICEDIWVTPGTTEIL
                     TLHGANLVINISASPYTIRKEKYRETMIQRIVKRCHMFLAFCNMVGGQDELVFDGYSM
                     IFNPEGEMLASAKTFEEDMIFAEIDPLESEAKRFTITVPRQIPPVEIKRLGDFKNRKR
                     TKAIKSVINPPPSREEEIYKALILGTTDYIQKNGFSEVVVGLSGGIDSAVTAVLAYDC
                     VGPERMHLVFMPSEYTQDQSTKDAKKLAQNLGIPLQTFPIDSTFQCYKEMLKGSFKGL
                     KEDITEENLQARIRGNILMALSNKFKWLVLTTGNKSEVSVGYSTLYGDTAGGFAVIKD
                     LYKTEVFKLARYYNRKEKRTVIPRSVIEKPPSAELRHNQKDTDSLPPYEVLDPILKLY
                     IEQDRGQKEIIDRGYDEELVNRIIRMVDLAEYKRRQSPPGVKITPKAFGRDRRMPITQ
                     RYFR"
BASE COUNT          991 a          966 c          937 g          994 t
ORIGIN      
        1 acagggatca tgccctacaa ttaattcaaa tgggagtttt attacgatgt cgccggatgt
       61 ttggctttga cttgctcttt gaccatatcc ttgagctttt ccggggggaa cttgccgcgg
      121 gtttctgatt tgacctgacc aataatatac tctatcagct tttcttcgga tttgaatctg
      181 tcgccatcaa gctctgcggc tttgctttct attagagagc ttacatcact ctcagtcatc
      241 ggtattaaat tcaattcatc gaaaagcctc tccagcggaa tattcgtgcc atttgatgaa
      301 agataatcga gagctacata gaccgcttcg tttggcaatt cacgttgtcc ggtttgctcc
      361 agaatgtagg ccaggagatc atcggaaata aatatcaggt ccctgccctg gcgtttcaga
      421 tttttcagat agccctcgaa aagatatgcc aggaaaaccg ggtcagtgag attgcgcttc
      481 aataaccggt cgtacataag tgcaaatttt gaacgtccca gggaatagac cagttcatcg
      541 gacagcccca gttccatcca gcgttcatcg cggctgtagg ccgggtccgg aagaaccgcc
      601 ctgagcttat ccagacgatc tttggtgatt gccaccggcg aggaatcggt gtcgggatac
      661 atacggtccg ggccgggaag tatccgttcg aaatccgtaa tgccctcgcc aatatgctga
      721 cgggtttcat tgggcacgcc ctcggtcgcc tccacagcac ggatcttcac ttccgacagc
      781 gccatgttga catcagcttc agtgccccag gttataataa tcgcatcggt atgacgcata
      841 tggaagacct ttttaatctc gtcgcagatt tcatccggca gttcggcaca actgatatcg
      901 tcggaatgga tgatgttcgg atatttatcg atgcaggcga ttaccttaat tcgtccggag
      961 atctcatcgg caaaggtctt attcggctgg gtcttgtaat tgagtatgcc cgcaaagcgt
     1021 tccagcatga taccgccaac cttgccgcca ttgtttatga cctcggatat caccggatgc
     1081 gctatcttct tgaaaatctc ggtcaggtcc ttcttctcgc tgtgaattgt actgggggat
     1141 attccgcgtt tgcgcagttc tgattttatc tccagaagcg atttttgcct taaaccctcg
     1201 acctcggtca ggcgcttgat atatccggtt ttatcaatgc ccttgagctc cacccgagtg
     1261 ccgccgtcga tggaaacatt gacatcctga cgggttactc caatcccgcg ccgtacccgt
     1321 cccgatgagc gcagcacgcg tccgataacc tcatcgactt ccatcgcctc ggtcggagtt
     1381 tgcatatccg gataggtcac aatctcgacc aaaggagttg aaagacgatc tgtcctgaac
     1441 ctgatctgat gtccaatatc gtgcacctcg cggcaggcat cctcctcaag agcgacttga
     1501 ataatcctga ttttgcggtc cttgtatggt atccaccctt ccacaccgac aatggcggtg
     1561 cgctggaagc ctgtggggat cgagccatcc agatactgct ttctggtaat atgaagttca
     1621 tccacaatat ggcagttgaa gagcatggaa atctggaggg cgatatcgag agcctcctgg
     1681 ttgatcggaa aaggcggggt atcatccatc tcataggtgc agacattatc ccggaagagc
     1741 tcgtaaacca cctctttttt ggtcttgaat tccatcaagg cggtgccgtc atactctccc
     1801 agttcggaaa gtgtggggcg catatgtctt acgattaaac cgtcgggagg accgtcaaca
     1861 taaccgaccg ggcagcggca gaacagcttt ttggcggtat cgagctgttg atgaacctcc
     1921 agcccacact tgaagccaag ctctttgaat ctgctgtcgt ctaaattatc ccagttcata
     1981 acttgtgaaa tatatcactt taatctctca atataccaaa tctacaggcc ttggcaaccg
     2041 atttcgtgtt tttttttgat gtttgacaac acgaccgaaa atgttatctt ttgcccttat
     2101 gaaaaggaag ttccgtatag cgctggccca gatcaactcc acggtgggag cgctggatca
     2161 aaacctggat aaaatcattt cttacgccag aagggccgag gagcatggct gcgatatagt
     2221 ggcctatccg gagctggccc tgaccggcta tccccctgag gacctgctcc tgaagaaggc
     2281 cttcaatctt gatgtttcca ggactcttaa gaagctgatt aaggaaaaga taaggccgat
     2341 catggtgttc ggttatcctg atttcgacgg cgggaagtat aattctgcag cggtaatcta
     2401 taataataag ctggtcgcct cggtcaataa attcatgctg cccaattacg gcgtctttga
     2461 tgaaaagcgc tatttcaccc ccggaaacgg aacatatata ttcgatctcg gcttcatgcg
     2521 tttcggtgta aatatctgtg aggatatctg ggtcactccc ggcaccacgg agattctgac
     2581 cttgcatggc gccaatctgg ttatcaatat ctccgcctct ccctacacta ttcgcaagga
     2641 aaaataccgc gaaaccatga tacagcggat agtaaagcga tgtcatatgt ttctggcctt
     2701 ctgcaatatg gttggcggtc aggatgaact ggtttttgac ggctattcga tgatattcaa
     2761 tcccgaaggg gagatgctgg cctctgcgaa gacattcgag gaggatatga tcttcgccga
     2821 aatcgaccct ctggagtccg aggccaaacg gttcacgatt acggttcccc ggcagatacc
     2881 tccggtggaa attaaaagac tgggcgattt caaaaaccgc aaaagaacta aagcaatcaa
     2941 atcagttata aatcccccac cgtcccggga ggaggaaata tacaaggctc tgattctggg
     3001 aacaactgat tatatccaga agaatggttt ttccgaagtc gttgtagggc tttccggcgg
     3061 gatagattcg gccgtgaccg ctgtcctggc ctacgactgc gtaggaccgg agcgaatgca
     3121 cttagtcttt atgccctcgg agtatactca ggatcaatcg accaaagacg ctaaaaagct
     3181 ggcccagaat ttgggaatac ctctccagac atttccgatt gacagcacat ttcaatgcta
     3241 caaggagatg ctcaagggga gtttcaaggg tcttaaggag gatattaccg aggaaaacct
     3301 tcaggcgcga atccgcggca atatcctgat ggcgctttcg aataaattca aatggctggt
     3361 gcttacgacc ggaaacaaat ccgaggtttc agtcggatac tccacactgt atggcgacac
     3421 tgccggcggt tttgcagtca taaaggattt atataaaacc gaggttttca agctggcgcg
     3481 ctactataat cgtaaggaga agcgaacggt gatacctcgt tcggtaatag agaagccgcc
     3541 ctcggcagaa ctgcgccaca accagaaaga caccgacagc cttcctccct atgaagtgct
     3601 cgatccgatt ctgaaactat acatagagca ggacaggggc caaaaggaga tcatagatcg
     3661 cgggtatgac gaggaacttg taaaccgtat cataaggatg gttgatctgg cagaatacaa
     3721 acgccgccag tccccgcccg gcgtcaagat tacccccaag gcctttggcc gcgatcgcag
     3781 aatgcctatc acacagcggt atttcagata aatcaattat ctgctgctga gtggaagaaa
     3841 aataatggcg tctcaatttt aaatgaaact ataagaccaa acctatgc
//
LOCUS       DSEB01000410            1963 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1010512, whole genome shotgun sequence.
ACCESSION   DSEB01000410 DSEB01000000
VERSION     DSEB01000410.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1010512"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            72..1415
                     /locus_tag="ENO07_07445"
     CDS             72..1415
                     /locus_tag="ENO07_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018001977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HDS01840.1"
                     /translation="MAVGDSRSTKIILIGILIGIILGGILGYLLGEDVLWSAFFGKLF
                     LNALKMIVIPLIITSMVTGVAGLGDIRKLGGTAAKTLGYYFLTTFASVTIGIICVNLI
                     QPGVGQGLFFIEQKDFVDAGEFSVFRFFLSLIPTNIFDAMARTDVLPLIIFSLFFGAV
                     LTTIGPKGQKLYEIFDGLNDVIMKMVHLIMYFAPIGVFGLVAGKIAEASMPMLDPGIY
                     QDLKTLIEFHGGHGVEYFIAYAEPLTRGGGHQIWLEVLKVGKYSLTVLTGLAVHAFIV
                     LPFVLSILARRNPLKYGYGVIQAVATAFSTASSSATLPITIDAVVKNNKVNSKAASFV
                     LPLGATINMDGTALYEAVAAIFIAQLYGIELSFAQQVIIVLTATLAAVGAAGIPQAGL
                     VTMVIVLQSVNLPVEGIASILAVDWLLDRFRTAVNVWGDSVGAAVIARTKEIRSA"
     gene            1532..>1963
                     /locus_tag="ENO07_07450"
     CDS             1532..>1963
                     /locus_tag="ENO07_07450"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDS01841.1"
                     /translation="MRVVVQRVRSARVSVENKIIGEIGPGLVLLVGAAEGDTGEDVRK
                     MAYKCLNMRIFEDKAGKMNLSCLKLGYDVLVISQFTLIADTRKGHRPSFSGALEPEKA
                     EGLYHEFVEACRAEGLKVAEGEFGAHMLVEINNWGPVTIIVD"
BASE COUNT          476 a          447 c          533 g          507 t
ORIGIN      
        1 cgattgacca aatgacatca attctttaag atagtatctt atattgacat gcaaccagca
       61 ttggatctat tatggcagta ggcgattcac gcagcaccaa aataattctg atcggaattc
      121 tgatagggat catcctgggc ggaattctcg gctacctgct gggcgaggat gtgctctggt
      181 cggcattctt cggtaaactc tttctgaatg cactcaagat gatcgtcata ccgctgataa
      241 taacctccat ggtaaccggc gtggccggcc tgggcgatat ccgcaaactg ggcggcaccg
      301 cagcaaagac gctggggtac tattttctca caaccttcgc ctctgttaca atagggatca
      361 tctgtgtcaa tctgatccag cccggagtgg ggcaggggct tttcttcata gagcagaaag
      421 actttgtgga tgcaggcgaa ttctcggtct tccgtttttt tctgagcctg ataccgacca
      481 atatttttga tgctatggcc cgcacggatg ttctccctct gatcattttc tcgctctttt
      541 tcggggcggt cctgacaacc ataggcccca aagggcagaa gctgtatgag atctttgacg
      601 gcctaaacga tgtaatcatg aaaatggttc accttataat gtattttgcg ccgatcggag
      661 tgtttgggct ggtggcaggg aaaatagcgg aggcctccat gccgatgctc gatccgggta
      721 tctatcagga tttgaagacg ttaatcgaat ttcatggcgg gcacggtgtg gaatacttta
      781 ttgcttatgc cgagcccttg accagaggcg ggggtcatca aatctggctc gaggttttga
      841 aggtcggcaa atattcattg accgtcctga ccgggctggc ggtacatgca ttcatagtgc
      901 tcccgtttgt gcttagtatc ctggccagga ggaatccatt gaagtacggt tacggtgtta
      961 tccaggccgt ggcgacagca ttctcgacag catcctcatc agccacgctg ccgataacaa
     1021 tagacgcggt agtcaaaaac aacaaggtga attcgaaagc cgcctcattc gttctgccgc
     1081 tgggcgcaac catcaatatg gatggcaccg ctctatatga agcagtggcc gccatcttca
     1141 tcgcgcagtt atatggcatt gaactgtcat ttgcacaaca ggttataata gtcctgacag
     1201 ccaccctggc ggcggtggga gcagcaggta taccccaggc aggtttggtc accatggtaa
     1261 tcgtgctgca atccgtgaat cttccggttg agggtattgc ttctattctt gcagtcgact
     1321 ggcttctgga cagattccgc accgccgtga acgtatgggg ggacagcgtg ggagcggcgg
     1381 tgatcgcacg caccaaagag attcgttcag cttagcagga aaaaaatact ggcaaatgaa
     1441 acatttcaag cctgaatggc cttagctaaa tacttgatat taattggttt ttgttgacaa
     1501 tcataaagca aaatcctaat ttgatttggt tatgagagtg gttgtgcaac gcgttagaag
     1561 cgcgcgagtc tcggtcgaaa acaaaatcat cggtgaaatc ggtccaggac ttgttcttct
     1621 ggttggcgcg gcggagggcg ataccggcga ggatgtccgg aagatggcat ataaatgcct
     1681 gaatatgagg atctttgagg acaaggcggg caaaatgaat ctctcctgcc tcaaactggg
     1741 ctacgatgtt ctggttattt ctcaattcac cctgatagcc gatactcgca agggccacag
     1801 gccctcattt tcaggggccc ttgagccgga aaaggccgag ggattgtatc atgaatttgt
     1861 ggaggcctgc agggctgaag ggctgaaagt tgccgagggg gaattcggcg cgcatatgct
     1921 ggtggaaata aataattggg gaccggttac aattattgta gac
//
LOCUS       DSEB01000411            2446 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1006478, whole genome shotgun sequence.
ACCESSION   DSEB01000411 DSEB01000000
VERSION     DSEB01000411.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1006478"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1287)
                     /gene="purH"
                     /locus_tag="ENO07_07455"
     CDS             complement(<1..1287)
                     /gene="purH"
                     /locus_tag="ENO07_07455"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:PRK00881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDS01842.1"
                     /translation="MTDGSKDKWIKIKRALISVSDKTGIVEFATRLQGLGVEIISTGG
                     TKKVLKENDIHVIPISSFTGAPEILGGRVKTLHPAVHAGLLHKRDDENHLKEMKEQNY
                     KAIDMVILNLYPFEQTVQKEDASEDEIIENIDIGGPTMLRAAAKAYQYVAIVTDPSQY
                     SRVIEEIEENTGSISFRTRQNLAREAFCRVSQYDNAISNYFNCKGQAEAETDFPSSIN
                     INVAKVTDLRYGENPHQKAALYKDPGTILPSLTKAEVLSGKALSYNNYTDLEAVLSML
                     MDFHKPFAVVVKHQNPCGAAEADTLQEAYRLALDADPISAYGSIIGLNKPVDMDTALR
                     LHETQFVECILAPDYDEGVVEKMKKKKARRILRLPRILEGYPDKFMQYSFIKGGLLAM
                     DPDLYEVKKEELKVVTEIQPTEDQIESLLFAFKVVKH"
     gene            complement(1302..2207)
                     /locus_tag="ENO07_07460"
     CDS             complement(1302..2207)
                     /locus_tag="ENO07_07460"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018123248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDS01843.1"
                     /translation="MADTLQTVYRTEISEFPKYADGKVRDVYDLGDKLLVVATDRLSA
                     FDVVLPNPIPQKGEVLTKMSVFWFDYLSDVVPNHFITANIDEYPHELQKHADTLKNRS
                     MLVKKCKRIDFECIVRGYITGSMWKEYQKIKPKDSKKILHGFVLPDDLEESEKLPKPL
                     FTPSTKAESGHDENISQEEMIESLGRDLVQKLEDVSIRLFEKASNYAESRGIIIADTK
                     FEFGFDGDELTLIDEVLSPDSSRFWPKYKYAKGRSQESFDKQFIRDYLTKAGWNKKPP
                     APEIPDDIIEKTSQKYKEAYELLIE"
     gene            complement(2200..>2446)
                     /locus_tag="ENO07_07465"
     CDS             complement(2200..>2446)
                     /locus_tag="ENO07_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDS01844.1"
                     /translation="KGMYGMHVHEAVLKAGERISGATVHQVNEKYDEGKILAQWEVPV
                     KEDDTPETLQKRVLETEHKLYPDTIAKIARGEIKLNG"
BASE COUNT          538 a          605 c          573 g          730 t
ORIGIN      
        1 atgtttcaca accttaaacg caaataataa tgattcaatt tgatcttctg taggttgaat
       61 ctctgtaacg actttgagtt cttctttttt gacctcatag agatcgggat ccatggccag
      121 gagcccgccc tttatgaagc tgtattgcat gaacttatcc ggatagccct ccagaatccg
      181 gggcagccgg agaattctcc gggctttctt cttcttcatt ttttccacca caccctcatc
      241 ataatccggc gccagaatac attccacaaa ctgggtttca tgcaggcgca gagccgtatc
      301 catgtcaacc ggcttattta agccgattat tgagccatag gcggaaatcg ggtctgcatc
      361 caatgccagc ctgtaggcct cctgaagcgt atccgcctca gcggcgccgc aggggttctg
      421 gtgcttgacc accaccgcaa acggcttatg gaaatccatg agcatggaca ggaccgcctc
      481 caggtcggtg tagttattgt atgacaaagc cttaccggaa agcacctccg cttttgtcaa
      541 tgatggcagg attgtgcccg gatctttata gagagccgcc ttctgatgcg gattttcacc
      601 gtagcgaagg tcggtgactt tggcgacatt gatatttatt gatgacggga aatcagtttc
      661 agcttcagct tggcctttac aattgaagta attcgagatg gcgttatcat actgggatac
      721 gcggcagaat gcttcgcggg ccaggttttg acgggtcctg aaagatatgg accctgtatt
      781 ttcctctatt tcctcaatga ccctggaata ctgggacggg tctgtaacga tggccacata
      841 ctggtatgct ttagcggctg cacggagcat ggtggggccg ccgatatcga tattttcgat
      901 tatctcgtct tccgaggcat cctctttctg cactgtttgt tcgaaagggt agaggtttaa
      961 aatgaccata tcaatggctt tatagttttg ttctttcatc tctttgagat gattctcgtc
     1021 gtcgcgttta tgcaagagcc ctgcatgaac cgcgggatgc agggtcttga cccgtccgcc
     1081 caggatttcc ggagcgccgg taaaggacga tatgggaatt acatggatgt cattttcctt
     1141 gagcaccttt ttggtgccgc cggttgaaat tatttcgact cccaggccct ggagcctggt
     1201 tgcgaattcg acaatccctg tcttatcgga gactgaaatc agagctctct tgatcttaat
     1261 ccatttgtct ttcgaaccgt cagtcataac atcaaatcct tctattcaat cagaagctcg
     1321 taagcttctt tgtatttttg gctggttttt tcgattatat cgtccggaat ttccggggcc
     1381 ggcggtttct tgttccagcc tgccttggta agataatccc ggataaactg tttgtcgaaa
     1441 ctttcctgac tgcgcccctt ggcatactta tacttgggcc agaaacgcga ggaatcgggg
     1501 gagaggacct catcgataag agtaagttca tccccgtcaa atccgaattc aaacttggtg
     1561 tcggcgatga taatcccccg tgattcggca tagttgctgg ctttttcaaa aagcctgatg
     1621 gatacatctt caagcttctg taccagatcc ctgcccaggc tctctatcat ttcctcctgc
     1681 gaaatattct catcatgccc gctttcggcc ttggtcgagg gggtaaaaag cggtttgggg
     1741 agtttttcgg attcctccag atcatcaggc agcacgaatc catgcaggat cttcttgctg
     1801 tctttgggct tgatcttctg gtattccttc cacattgagc cggtaatgta acccctgaca
     1861 atacattcga agtcgatgcg cttgcatttc ttgaccagca tggaccggtt tttcaatgtg
     1921 tccgcatgtt tctggagctc gtgagggtat tcatcgatat ttgcagtaat gaaatggttg
     1981 ggaacgacat cactgagata atcgaaccag aaaaccgaca ttttagtcag cacctcgccc
     2041 ttttgaggta tcgggttggg aagaaccaca tcaaaggccg agagcctgtc tgtggcgact
     2101 accagcagct tatcgccgag atcataaaca tcacgtacct tgccgtcggc atatttggga
     2161 aattcactta tctctgttcg atagacggtt tgtagagtat cagccattta acttaatttc
     2221 tcctcttgca atttttgcga ttgtatccgg atataattta tgttctgttt cgagcactct
     2281 tttttgaagc gtttcgggag tgtcgtcctc cttgaccggc acctcccatt gagcaagtat
     2341 cttgccttca tcgtatttct cattgacctg gtgcaccgtt gccccgctga tcctttcacc
     2401 tgccttgagt acggcctcgt gcacatgcat gccatacatc cccttg
//
LOCUS       DSEB01000412            1605 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1016483, whole genome shotgun sequence.
ACCESSION   DSEB01000412 DSEB01000000
VERSION     DSEB01000412.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1016483"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..954)
                     /gene="corA"
                     /locus_tag="ENO07_07470"
     CDS             complement(<1..954)
                     /gene="corA"
                     /locus_tag="ENO07_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDS01845.1"
                     /translation="MTRLIKKHSKSKGLKPGSVVFVGEQRTEKATIEVIDYSETELQE
                     IRLEKIEDSFQYKDTSTITWININGIHEVGNIEKLGEHFGIHALTQEDIANTGHRPKL
                     EDSGEYIFVTLKMLYQKSEDGIESEQVSIVFGKNYVLSFQEKEGDVFEAVRERIRRTE
                     PRVRFMGSDYLAYTLMDAIVDNYFIILENIGDTVEELEDDLVSNPEPEDLETIYDLKR
                     QMVFIRKAIWPLREVASGMDRAESVLIHDFTRPYLRDLYEHIIQVIDTVETFRDMISG
                     LLDIYLSSISNRMNQVMKVLTIIATIFIPLGFLAGVYGMNFD"
BASE COUNT          398 a          444 c          303 g          460 t
ORIGIN      
        1 atcaaagttc ataccgtaaa cacccgccaa aaatccaagc gggatgaata tcgtcgcaat
       61 tatggtcagg actttcatta cctggttcat gcggttgctg atactggaaa ggtatatatc
      121 cagaagcccc gaaatcatat cacgaaaagt ctcaaccgta tctattacct gtattatatg
      181 ctcatataag tcccgcagat agggcctggt gaaatcatgg atcagtaccg attcagccct
      241 gtccatgccg ctggcaactt cgcgcagggg ccagattgcc ttgcgaatga aaaccatctg
      301 gcgcttcaaa tcatagatcg tctccaaatc ctcgggctcc ggatttgaaa ccagatcgtc
      361 ttccagttcc tccacagtat ccccgatatt ctcgagaatg ataaaataat tgtcaacaat
      421 agcatccatc aaagtatatg cgaggtaatc cgaccccata aacctgaccc gaggttcggt
      481 cctgcggatt ctttcccgta ccgcttcaaa aacatcacct tccttctcct gaaaagagag
      541 cacataattc ttgccgaata caatgctgac ctgctcggat tcaatgccgt cctccgattt
      601 ctggtaaagc atcttcaagg taacaaagat atactcaccg ctatcctcca gcttgggacg
      661 atgtccggtg ttggcaatat cctcctgcgt cagggcatga attccgaaat gctcacccag
      721 tttttcaata ttgccgacct catgaatgcc gtttatattt atccaggtta tagtggaggt
      781 atctttatac tgaaagctgt cttcgatttt ttccagcctt atttcctgca attcggtttc
      841 actgtaatcg ataacttcaa ttgttgcctt ctctgttctc tgttcaccaa caaagaccac
      901 agatccgggc ttcagccctt ttgatttcga atgtttcttg attaaacgag tcatatcatt
      961 agtaccccgt tacaaatatt gcggcggttc ggcaaatcta cttgtctttc ttctcttctt
     1021 ccttccgctt cagaatctcg gcgagattct cacgccggtt ctctatccgc tttaggagcc
     1081 ccttcatatt ctcgacgtcc gaatcggatc cggctttgtc gatactctcc ttgagtttat
     1141 ccatttccct gcccaatttc tcatatgtct cccgcagctt ttcaatcgac tcggcctcgt
     1201 cagccttctc aaaaacgacc ttctcgggct gtgccatagc ttctgataca gcctgcccct
     1261 cagaaggcgc ggtatccgga ataattttct tcccctccgg gacagctctc gtactcataa
     1321 atgtgggtga tactacttca acgccatttt gctgcaatac atcgagcatc atcttgcgca
     1381 gccgtgagcg ggctgaaatt agatgtttga catctttcag cagtccggag atacgatatg
     1441 tcaccgaaaa atctcccaga tcaattacat gcataaacgg ctcctccagc tctgtagcct
     1501 ccgccgcctg caaaagcaga ttctcgatct tatttctgga gacattgtaa cccaatgaga
     1561 catcggctga aataatcgtc cccgatgacc tcaccacctt aacag
//
LOCUS       DSEB01000413            2447 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1006470, whole genome shotgun sequence.
ACCESSION   DSEB01000413 DSEB01000000
VERSION     DSEB01000413.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1006470"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1794
                     /locus_tag="ENO07_07475"
     CDS             <1..1794
                     /locus_tag="ENO07_07475"
                     /inference="COORDINATES: protein motif:HMM:PF13727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDS01846.1"
                     /translation="VSYWGAFQLRFGGNLGQTELNVFTNTLFMVVIVRFTLFAYFKVY
                     SGSYKYASIDDLLQIIKAVGIGSMALVIPIFFIPIMGFPRSVLLIDSLILIILTGGLR
                     LAIRFGRELMPHFLRQGRRVLIIGAGDAGEMIIREMKRSRALNSNPVAIIDDDIQKKG
                     TYIHGVPVVGDQTDIGKVVDKYNIEEIIIAIPSATNRQMRRIIRNCRKLKVSFKTVPP
                     LKDIINDTVSIHQVRDIRVTDLMGREPLNLNIDLISRFINGKRILVTGSAGSIGSEIC
                     RQIMNFDPAEVIMVDRAENNLYNLQQELRSHTEINKVFIIADINNLSKMRTLFEEYNP
                     EIVYHAAAFKHVPLMEEFPEEAVMNNIFGTKIVLDLALEFKVSHFILISTDKAVAPTS
                     VMGASKKVTELLAGIAGLSPVTKVISVRFGNVLDTDGSVVPLFRKQIEKGGPVTVTDP
                     RVSRYFMTISEATLLVLEASAMGEGGQLFVLKMGEPIRILDLAKDMITLAGLVPNEDI
                     EIEFVGLRPGEKLHEELFIEEDRLVSKNHKKMLIAEPDVCDRESLENALIDLHHLCKK
                     TDRGGIIAKLQEIVPNYTPLDSTIKNKEVKG"
     gene            1795..>2447
                     /locus_tag="ENO07_07480"
     CDS             1795..>2447
                     /locus_tag="ENO07_07480"
                     /inference="COORDINATES: protein motif:HMM:PF02403.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDS01847.1"
                     /translation="MLDLKFISENAELVRKAIKDKHENANIDKILELDNQRKDLIQDV
                     ESKKAQRNKATSEIAKLKKSGQDASSLIGEMKKLGEEIAGLDDRRRDVENELKELLLT
                     IPNIPDSDTPVGDSEDDNVEIRSWGRQPKFNFTPAPHWELGEKLGLFDLPAGAKISGS
                     GFILYTGRGARLQRALINFMLDLHTNEHNYKEVSPPFMVTRQTMTGTGQLPKLEEDM"
BASE COUNT          660 a          565 c          638 g          584 t
ORIGIN      
        1 gtctcctact ggggggcgtt ccagcttcgc ttcggcggga atctggggca gactgaactg
       61 aatgtcttta ccaatacctt gttcatggtc gtgattgtgc gcttcacact ttttgcctac
      121 ttcaaggtat attccggctc atataaatat gcctccattg acgatctgct tcagattatt
      181 aaagctgtgg gtatcggctc gatggcgctg gttatcccga tctttttcat acctatcatg
      241 ggcttcccgc gctcggttct tttgatcgat tcgctgattc tgataatcct gaccggcggt
      301 ctcaggctgg caataagatt cggccgggaa ttgatgcccc atttcctccg tcagggcagg
      361 cgtgtgctga taatcggcgc gggcgatgcg ggggaaatga ttatccggga gatgaagcgt
      421 tcccgggcat tgaactcgaa tccggtggcg attatagatg acgatattca gaaaaaaggc
      481 acctatatcc acggcgtacc ggtggtgggg gatcagaccg acatcggcaa ggtggttgac
      541 aaatataata tcgaagaaat catcatagcc ataccctcgg ccaccaatcg ccagatgcgc
      601 aggattatcc gcaattgccg caagctgaag gtaagtttca agactgttcc gccgctcaag
      661 gatatcatta atgatactgt ctcgatccat caggtacgtg acattcgtgt gaccgatttg
      721 atgggacggg aaccactgaa cctcaatatt gatctgatca gcagattcat aaatggtaaa
      781 cgtattctgg tgacaggctc tgccggttcg atcggctcag aaatctgccg ccagatcatg
      841 aacttcgacc cggccgaggt gataatggtg gatcgggccg agaataatct gtacaatctc
      901 cagcaggagc tcaggagtca cactgaaatc aacaaggtct ttattatagc cgatataaac
      961 aacctttcca agatgcgcac gctctttgaa gaatataatc cggaaattgt atatcatgcc
     1021 gccgccttca agcatgtccc cctgatggag gaatttcccg aggaggcggt gatgaacaac
     1081 atcttcggta caaaaatagt cctcgatctt gcgctggaat tcaaggtttc gcacttcatc
     1141 cttatatcta ccgataaggc ggttgccccc acctcggtta tgggcgcctc caagaaagtg
     1201 accgagcttc tggccggaat cgccgggctc agcccggtta ccaaagtcat cagcgtacgg
     1261 ttcggaaatg ttctcgatac ggatggctct gtggtgccgc ttttccgcaa gcagatagag
     1321 aagggcggac cggttacggt gaccgatccc cgggtaagcc gctatttcat gactatcagc
     1381 gaggcaacgc tgctggttct ggaagcctca gctatggggg aggggggcca gctgtttgtg
     1441 ctcaagatgg gcgagccaat cagaattctg gacctggcca aggatatgat cacgctggcg
     1501 ggtttggtgc ccaatgaaga tattgaaatt gagtttgtcg gtttaaggcc cggggaaaaa
     1561 ctgcatgaag aattgtttat agaagaggac aggctggtct cgaagaatca taaaaagatg
     1621 ctcatcgccg agcccgacgt ctgcgacaga gaaagcctcg agaacgctct catcgatctt
     1681 catcatctct gcaagaaaac agatagaggc ggaattattg caaaattgca ggagatagtt
     1741 cccaattata caccgctgga tagtacaatc aagaataaag aggtaaaggg ttagatgctg
     1801 gatctgaaat ttatttctga aaatgctgag ttggtcagaa aggccataaa agataaacat
     1861 gaaaatgcga atattgacaa gattcttgaa ctcgataatc agcgcaaaga tttgattcaa
     1921 gatgttgaaa gtaaaaaggc ccagagaaac aaggccacat ctgaaatcgc aaagttgaag
     1981 aagtccgggc aggatgcttc ctcactgatc ggcgagatga aaaaactggg agaagagatt
     2041 gccgggcttg acgaccgccg ccgtgacgtc gaaaacgaac tcaaggaact gctgctgact
     2101 atccccaata ttcccgattc ggatactcct gtcggcgatt ctgaagatga taatgtggaa
     2161 ataagaagct gggggaggca accgaaattc aattttactc cggcccctca ttgggagtta
     2221 ggcgaaaagc tgggactgtt tgaccttcct gcgggagcca agatttcagg cagcggattt
     2281 attctctaca ccggcagggg agcccgtctg caaagagcgc tgataaattt catgctcgat
     2341 ctgcacacaa atgaacataa ttacaaagaa gtctctccgc cctttatggt aacccgccag
     2401 actatgaccg gcaccgggca attacccaag ctggaagagg atatgta
//
LOCUS       DSEB01000414            4073 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002094, whole genome shotgun sequence.
ACCESSION   DSEB01000414 DSEB01000000
VERSION     DSEB01000414.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002094"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(318..806)
                     /locus_tag="ENO07_07485"
     CDS             complement(318..806)
                     /locus_tag="ENO07_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01848.1"
                     /translation="MSSAYLHILLNHVPVIGILIGLAILITGLVFKKALLIKTSLAML
                     LIFAVFAVPTYLTGEPAEEEIEHQPGVSEEMAHEHEEAAELAFIVSLIMGALALISLA
                     VWKVRSKMPSWTVIATLLVAILSAALMLWTAHLGGKVRRPELRDQEYPQETPDVYKSS
                     QK"
     gene            complement(837..1589)
                     /locus_tag="ENO07_07490"
     CDS             complement(837..1589)
                     /locus_tag="ENO07_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013287972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDS01849.1"
                     /translation="MESKVVLVTGASRGIGKAVAKRFAEKGYRVIVHYNKNKAGAEKT
                     LAELPGKSHQVSQADLADSESLQAMVNNVMQAMGRVDVLVNNAGVFIEHPPAETSFED
                     WQKAWKETIYTNLIGPADLAYFVARQMIKNAGGRIINISSRGAFRGEPDAPAYGASKA
                     GMNSMSQSLAKALAPLKIFVYTVAPGYVETDMVKDTLEGPGGDEVRAQSPLNRAASPD
                     EIAGLVEYLASDAPEYMTGSIVDINGASYLRN"
     gene            complement(1769..3772)
                     /locus_tag="ENO07_07495"
     CDS             complement(1769..3772)
                     /locus_tag="ENO07_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter KefB"
                     /protein_id="HDS01850.1"
                     /translation="MGEIPILQDIVVIFGCSVVVLFICHRLGIPSIVGFLVTGLLIGP
                     SALGIVRSIHDVEVLAEIGVILLLFSIGIEFSFRSLLRSKRLVMLGGTLQVLLTIALA
                     YLVAIQLGQEPSRAFFIGCLIALSSTAIVLRVLQEKVEIASPHGRVVLSILIFQDIII
                     VPMMIFTPLLAGAEGHTGSSPLMLVVKALAVIILVVLTARYLVPGILYQITRTQSREL
                     FLLSIVVIGMAVAWLTSQAGLSLGLGAFLAGLVISESEYSHRALEGILPFKEVFTSFF
                     FVSVGMLLDISFFLENPFLIAGGAVLVLFVKSGLAAGVALLLGMSSRAALIAGLSLGQ
                     VGEFSFILSRVGLEFNLIDGDLYQRFIAVSILTMGVTPFIMSAAPGLAERMSDWPFLR
                     KYKSGSYIAMGENKHVSAEALDDHLIIIGFGINGRNVARAARASNIDYRVIEMNPDTV
                     NQEREKGEPIFYGDATGEAALHQAAIKKARILVITIADPSATRRITALARRMNPRVHI
                     IVRTRFVQEMKPLYDLGADNVIPEEFETSVEIFTQVLMKYLVPREEIDKFIDEVRADS
                     YQMFRGLSRRTSSVSDLKRHLSDIDVCSLRVDKKAELAGKTLMETDLRRKHGITVIAI
                     QRGDRVITSPDGPSRIEAEDTLYVLGDVEHISRFQKLIKAGKK"
BASE COUNT         1069 a         1103 c          901 g         1000 t
ORIGIN      
        1 agttcaccca gagaggtctc cctgtttttc ataattgatt gcaatagaag gagttgcggt
       61 cccgtaaggc tgtagtcctg gacaagccgc cgagaatgca gatcaattgc gcgcataata
      121 cgtctgagcg ccacaatcac ctgatccgag tattttattt ccgtatcaac catcttttct
      181 atacaaatat tttgcacgct aaatatctac gaattaatta ataagtcaag ataatttttc
      241 atagaaaatt agcccttcat caactgttct taatctataa catgatgtaa gcaaagaagt
      301 tccgggatgg cccgggctta tttctggctg cttttgtaaa catcgggtgt ttcttgagga
      361 tattcctgat cccgtaattc cggccggcga accttccctc ccagatgcgc tgtccagagc
      421 atcagagcgg cgctcaatat tgccaccagc agggtagcga tcactgtcca ggagggcatc
      481 ttcgagcgga ctttccatac tgcaagcgaa atcagcgcca gtgcgcccat gataagcgag
      541 acaataaagg caagctcggc tgcctcctcg tgttcatgag ccatctcctc agacacccct
      601 ggctggtgtt cgatttcctc ctctgccggt tcccccgtca aataagtggg aactgcaaaa
      661 actgcaaata ttagaagcat ggccagagac gttttaataa gcagggcctt tttgaaaacc
      721 agacctgtaa tcaaaattgc aagcccgatc aaaataccaa ttacaggaac gtgattcaat
      781 aagatatgaa ggtatgccga actcatatta ctcccctttg ttcattattc cagattttaa
      841 tttctaagat aagaagcccc attgatatcg acaatgcttc cggtcatata ttccggagca
      901 tccgaagcaa ggtattcaac cagtccggcg atctcatccg ggcttgcggc gcgattcaac
      961 gggctctggg cgcgcacttc atcccccccg ggaccctcca gagtatcctt aaccatatcc
     1021 gtttcgacat atccgggagc aactgtatat acgaatatct tgaggggtgc caacgctttg
     1081 gccagggatt ggctcatgga attcatacct gctttgctgg cgccataggc aggggcatcc
     1141 ggctcgcccc gaaatgctcc acgtgaagaa atattgatga tcctccctcc tgcattctta
     1201 atcatttgcc gggcaacaaa ataggcaaga tcggcggggc cgataaggtt ggtgtaaatt
     1261 gtctctttcc aggccttctg ccagtcctcg aagctggttt ccgccggggg atgttcgata
     1321 aaaaccccgg cattgttcac cagcacatca actcttccca tagcctgcat gacattatta
     1381 accatcgcct gcaacgattc tgaatccgcc agatccgcct gcgagacctg atgagattta
     1441 cccggaagtt cagccagagt tttttcggcc ccggccttgt tcttattata atgcactatg
     1501 acccgatagc ctttctcagc aaatcttttc gcaacagcct tacctattcc ccgtgatgct
     1561 cctgttacca atacaacttt gctttccaaa tcgacgccca tactgtctcc tcccgatgga
     1621 tttaccagac aagctcttga ttgtggcgcc ctccattcag ccgccagtaa tttaaacctg
     1681 tcctgatact atgtcaaacc ttgaaatgcc aaaaatttcg gcttgttccc cctgagtcag
     1741 caccaccagc cttgacagag aagcgggact atttcttgcc ggccttaatc agtttctgaa
     1801 acctgcttat atgctccaca tctcccaaga catagagcgt atcttcggct tcaatcctgg
     1861 atggcccatc cgggctggtg ataaccctgt cccccctctg aatggcaatc actgtaatcc
     1921 catgcttcct tctgagatca gtctccatca atgttttccc ggctaattcg gcttttttat
     1981 caaccctgag cgagcagaca tcaatatccg aaagatggcg cttgaggtca gagacactgg
     2041 aagtcctgcg tgaaagcccg cggaacatct ggtaactgtc cgccctgacc tcatctatga
     2101 atttatcgat ctcctcgcgc ggcaccaggt atttcatcag cacctgagtg aaaatctcga
     2161 ctgatgtctc gaattcctct ggtatcacat tatccgctcc caggtcatat aacggtttca
     2221 tttcctgcac aaaacgtgtc ctgacaatta tatgaacacg cgggttcatg cgccgggcca
     2281 gcgcggtaat gcgccgggta gcgctgggat cggcaatcgt aataacgaga atgcgcgcct
     2341 ttttaatggc ggcctggtgc agggcggcct cgcctgtggc atcaccgtaa aatatcggct
     2401 cacccttctc ccgctcctgg ttgactgtat ccggattcat ttcaattact ctgtaatcta
     2461 tattcgaggc cctggctgca cgggcaacat tgcgaccatt gatcccaaag ccgattatta
     2521 taaggtgatc atccagggct tcggcagaaa catgcttatt ttctcccatt gcaatatatg
     2581 atccgctttt gtatttcctc aggaagggcc agtccgacat cctctccgca aggcccgggg
     2641 cggcagacat gatgaaaggc gtgacaccca tggttagaat cgaaaccgct ataaatctct
     2701 gatatagatc gccgtcgatc aggttaaact ccagtccgac cctggaaaga ataaacgaaa
     2761 attcacctac ctggcccagg gaaagtccgg caattaaagc cgcacggctg gacatcccca
     2821 gaagcaaagc cactcctgcg gccaatccgc tttttacgaa aaggaccaac acagcaccgc
     2881 cggcaattag aaacggattt tccagaaaga acgatatatc cagaagcatg cccacggata
     2941 caaagaagaa acttgtgaag acctccttga agggcaggat tccctccagc gctctatggc
     3001 tgtattcgga ttcggagatt accaggcccg caagaaatgc tcccagtccc agcgacaagc
     3061 cggcctgtga tgtcagccag gccacggcca ttccgattac aactatgctc aatagaaaga
     3121 gttcgcggct ctgggtacgg gttatctggt ataatattcc cggcaccagg tagcgcgcgg
     3181 tcagaactac aagtataata accgccaggg ccttgacaac aagcattaag ggcgatgatc
     3241 cggtatgacc ctcagccccg gccaggagag gggtgaaaat catcatcggc actatgatta
     3301 tatcctggaa gatcaatatt gaaagtacca cccgtccatg cggactggcg atttcgacct
     3361 tttcctggag cacacgcagg acaatcgcgg tgctgctcaa tgctatcagg cagccgatga
     3421 aaaaggcccg ggagggctcc tgcccgagct gtatggccac caggtaggca agtgctattg
     3481 tcagcaacac ctgcaatgta ccccccagca tcaccaggcg tttcgagcgc agcaggctgc
     3541 ggaatgaaaa ctcgattcca atcgaaaaca ggagcaggat cacgccaatc tccgccagaa
     3601 cctctacatc atgtattgac ctgacgatgc caagcgcaga tggcccgatt aatagcccgg
     3661 ttaccagaaa tccaacgatc gagggtatgc ccaggcgatg acatataaag aggacgacga
     3721 cggaacaccc aaaaattaca actatgtcct ggagaatcgg aatttcaccc actcaggagt
     3781 tcctttcaaa tactttcgcg ccatgttttc ggtggaacaa ccgaaaccgt tcaccccaag
     3841 ttaattaaga tcagcttttg attacaagta aaaaagctgt ttaatttgga tttcaggtat
     3901 acccgggacg acctatgccc tcgatgatcg tctgtatcgg cagggaggac ggcaaattat
     3961 ttacaacaaa tgtattgcca tattttcaac acttatataa tttgtttaag ccttgataaa
     4021 aaactataat ccggggccga tatgaataga cctgaatcat atcatgctaa tac
//
LOCUS       DSEB01000415            4071 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002097, whole genome shotgun sequence.
ACCESSION   DSEB01000415 DSEB01000000
VERSION     DSEB01000415.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002097"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1088
                     /gene="purD"
                     /locus_tag="ENO07_07500"
     CDS             <1..1088
                     /gene="purD"
                     /locus_tag="ENO07_07500"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:PF02844.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDS01851.1"
                     /translation="PEQPLSKGIVDLFQSKGLKIFGPTKKAAIIESSKAWAKEFMSKY
                     HIPTAPYRIFDNKDDASDFMAELQLPFVIKADGLAAGKGAIIVHTREEAEETLKDIMV
                     ERKFGEAGNRVIIEEKLEGEELSLLALTDGKSVLPLIPSQDHKAIYDDDEGPNTGGMG
                     AYAPVPFVSGRLQESILDLVFEPAIRGLAQEGRPFKGVLYAGLMITDFGPKVIEFNCR
                     FGDPETQVILPLLENDLAELCLAVANQDLQTDELKFKDNYCVGVVMAAGGYPGNYEKG
                     LPIQGLLKAQTEGRLVFHAGTEMKDGQVVTSGGRVLTVCAMADNLQDAIASAYEGVEK
                     ISFDNAHFRKDIAARASRRKFKLSRQV"
     gene            1089..1547
                     /gene="purE"
                     /locus_tag="ENO07_07505"
     CDS             1089..1547
                     /gene="purE"
                     /locus_tag="ENO07_07505"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDS01852.1"
                     /translation="MADVLIILGSKSDMKYGEKCQETLDKLDISNELVISSAHREPEK
                     TAQLASGAKANGTKVIICMAGMSAALPGVTAAKTDLPVIGVPLSGSALMGMDSLFSVV
                     QMPKGVPVGAVGLDKAGAENSALLAARIIALSDSNVYNKLNEYRDEMLKK"
     gene            1573..2490
                     /locus_tag="ENO07_07510"
     CDS             1573..2490
                     /locus_tag="ENO07_07510"
                     /inference="COORDINATES: protein motif:HMM:PF13432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDS01853.1"
                     /translation="MITKTVVMKIFLAAVLGLLIAGTSPLAAQDNTAAEHFNNGVDAE
                     AAGNTAKAIEEYRAAIAADSSFASPYYNLGVIYYSQKKFTDALKMFERVTQLEPDNLD
                     AWKNMGLSATETGDLSKGEKAFTEALKIKSNDPELTELFAMLFYKNEAYKRAIAKLED
                     LVKLKNQDKKTFYALGKSYKEAGNNEQAIVNLQSATKIDPEYQMAYFELGNIYLEDEN
                     WSKAMQNYEKSVQYDPDHYQSWFNLGSACVGESTESSIVRAYEAYQKFLSLTNGRGGS
                     QIKSMRNTASNISAQLRDYFDQAGIIYDE"
     gene            2709..3734
                     /locus_tag="ENO07_07515"
     CDS             2709..3734
                     /locus_tag="ENO07_07515"
                     /inference="COORDINATES: protein motif:HMM:PF00291.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDS01854.1"
                     /translation="MKYVNKITDVIGNTPLLKLGKTVAHLKPTIFMKLEYLNPAGAVK
                     DRMAYYIVKEGEKSGKLKKGDTLVDNSSGNAAIATSMVAAALGYKAVFAVADKTSREK
                     IDLIKAYGADVIITPTHVPWDDPRSSYMRAYELGQKPGYFYISQYHNQLNVEAHYKHT
                     GPEIWEDTNGKITHFVAGIGTGGTISGAGKFLKEKNPDIKVIGVDPEGSMFYDWVNNG
                     TTEGLELYPCKVEGIGTDMIVKAFHPEYVDYVIRAWDKDSFRVARQLAREEGILGGGT
                     TGAHLWAAMEIAKELDESAIIVTLACDSGQRYLSKMFSDEWMREQGFDIEEKGHATVS
                     LEQGRQI"
     gene            3731..>4071
                     /locus_tag="ENO07_07520"
     CDS             3731..>4071
                     /locus_tag="ENO07_07520"
                     /inference="COORDINATES: protein motif:HMM:PF07610.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HDS01855.1"
                     /translation="MNNIKTVIITTLAIPFLAGSGLFAQGYASDIFLPDSIFDFGFFA
                     TDARVVHTYPIVNRGNDTLRITKVRPGCGCTVAPLDKSNIAPGDTAYLDMYFDSKRLT
                     GLVKKGITILS"
BASE COUNT         1141 a          892 c         1096 g          942 t
ORIGIN      
        1 gtcccgaaca gcccctgtcg aaagggattg tcgatctgtt tcaatcgaaa ggcttgaaaa
       61 tcttcgggcc gacaaaaaaa gcggcaatca ttgagagcag caaggcctgg gccaaggagt
      121 ttatgtcaaa atatcatatt cccaccgcgc catacagaat ttttgataat aaagatgatg
      181 cctcggattt tatggcggag cttcagctcc catttgtgat aaaggctgat ggcctggcgg
      241 ctggcaaggg cgccataatc gtgcacaccc gggaagaagc cgaggagacc ctcaaggata
      301 ttatggtgga gagaaaattc ggggaagccg gcaatcgagt tattatagag gagaagctgg
      361 agggcgagga attgtcgcta ttggcgctca ctgatggaaa atcggtgctg ccgttgattc
      421 cctcccagga tcataaggcc atatatgatg acgatgaggg gcccaatacc ggtggaatgg
      481 gcgcttacgc cccggtcccg tttgtaagcg gccgacttca ggaatcgatt ctcgatttgg
      541 tcttcgagcc ggccatacgg ggtctggccc aggaggggcg accattcaaa ggagtcctgt
      601 atgcgggcct gatgatcacc gatttcgggc ccaaagttat cgagttcaac tgccgtttcg
      661 gcgatccgga aacacaggta atactccccc tcctggaaaa tgacctggcc gaactttgcc
      721 tggcggttgc caatcaggat ctgcaaaccg atgaattgaa gtttaaggat aattattgcg
      781 tgggcgttgt catggctgcc ggaggttatc cgggcaatta tgaaaagggc ctgccgattc
      841 aagggctcct gaaggctcag actgaagggc gcctggtttt tcatgcaggc acggagatga
      901 aagacggcca ggtggtcacc tcgggcggac gggttctcac cgtatgcgcg atggccgata
      961 atcttcagga tgcaatcgcg agcgcctacg agggagttga gaaaataagc tttgataatg
     1021 cacatttcag aaaagatatc gccgccagag cttcaagaag gaaatttaaa ctgtcaaggc
     1081 aggtgtaaat ggctgacgta ttaataattc tcggatcgaa atcggacatg aaatatggcg
     1141 aaaagtgcca ggagaccctg gacaagctgg atatttccaa tgagctggta atttcctccg
     1201 ctcaccgtga accggaaaaa accgcgcagc tggcctccgg ggcaaaagcc aatggaacca
     1261 aagtgataat ctgcatggcc ggcatgtcgg ccgccctgcc gggcgttaca gcggccaaaa
     1321 cggatctgcc ggtaatcggg gtgccgcttt cgggttccgc cttaatgggt atggattctc
     1381 tattttctgt ggtgcagatg cccaagggcg tacctgttgg cgcagttgga cttgacaagg
     1441 ctggagcgga aaattctgcc cttctggcag caagaattat cgccctcagc gattctaatg
     1501 tgtataacaa attaaatgaa tatcgagatg aaatgttgaa aaaatagatc atcaggaaag
     1561 gagactattc aagtgattac taagacggta gtgatgaaaa tattcctggc tgcagttctg
     1621 ggattattaa tcgctggaac ctccccgctt gcggcgcagg acaacacggc cgccgagcac
     1681 tttaataatg gggtggatgc ggaagccgcg ggaaatacgg cgaaggcgat cgaggaatat
     1741 agggcggcta ttgcggccga ctccagtttc gccagccctt attataatct cggcgtcatt
     1801 tattattcgc agaagaaatt cacggatgca ttgaagatgt ttgaaagggt gacccagctt
     1861 gaaccggata atcttgatgc ctggaaaaat atgggactct ctgcaaccga aaccggcgac
     1921 ctctcaaaag gcgagaaagc gtttacggag gcactgaaaa taaagtccaa tgatcccgag
     1981 ctgaccgagc tttttgccat gctgttttac aagaatgaag cttataagag agccattgca
     2041 aagctcgaag atctggtcaa gttgaaaaac caggacaaaa agactttcta cgccctgggt
     2101 aaatcttaca aagaggccgg caataatgag caggctattg tgaatctcca gagcgcaacc
     2161 aagattgatc ctgagtacca gatggcttat tttgaactgg gtaacatata ccttgaggat
     2221 gaaaattgga gcaaggccat gcagaattac gagaagtctg tgcaatatga tcccgaccat
     2281 tatcagtcgt ggttcaatct gggatcggcc tgcgtgggcg aatctaccga gagctctatc
     2341 gtcagggcat acgaagcata tcagaaattc ctcagtctta caaatggcag gggcggttcg
     2401 cagatcaaat cgatgagaaa caccgcaagt aatatctcgg cccagctgag ggactatttt
     2461 gatcaggcag gaattattta cgacgaatag aatgtaagat attaattcat acgggccttc
     2521 ccccagcggg aggccttttt tgttagtatt tttgccaata aaatattgca tttggtcaaa
     2581 aaagtctttt ataatacctc cctgtctgta aatagagtca gctctgctta ataaaatgag
     2641 gataggaaac caaggttaca ttatcaggta taaggtatta agagcgaaag gaatctggag
     2701 ttaagcgcat gaaatatgtc aataaaatta ccgatgtaat cggtaatacg cctcttttaa
     2761 aactggggaa aacagtagcc catttaaaac caacgatctt catgaagctt gaatatttaa
     2821 atcccgccgg tgcggtcaag gatcgcatgg cttattatat cgtcaaggaa ggcgagaaat
     2881 ccggcaagct aaaaaagggg gatacgctgg ttgataattc ctcgggcaac gccgcgattg
     2941 caaccagtat ggtagccgcc gcactgggat acaaagcagt ctttgctgtg gcggataaga
     3001 ccagccgtga gaaaatagat ctgattaagg cttatggggc tgatgttatc attaccccca
     3061 cccacgttcc ctgggatgat cccagaagca gctacatgcg agcctatgag ctgggccaga
     3121 agccgggata tttctatatt tcgcagtatc ataaccagct caatgtggag gcccactata
     3181 aacacaccgg gccggaaatc tgggaagata ccaatggaaa aattacgcat tttgtggcag
     3241 ggatcggaac cggcgggaca atttccggcg cgggcaaatt cctgaaggaa aaaaatcctg
     3301 atatcaaggt catcggcgtc gatccggagg gctccatgtt ttatgactgg gtcaataacg
     3361 gcaccacaga aggtcttgag ctctaccctt gcaaggtcga gggaatcggc accgatatga
     3421 ttgtgaaggc cttccatccc gagtatgttg actacgttat aagggcctgg gataaggatt
     3481 cattcagggt cgcccggcag cttgcgcgcg aggagggaat tctgggaggc ggcactaccg
     3541 gcgcacatct ctgggcagcc atggaaatag ctaaagagct ggatgaaagc gcgataatcg
     3601 tgacactcgc ttgtgactcg ggacagagat atttgagtaa aatgttttcg gatgaatgga
     3661 tgcgggaaca gggttttgat atagaggaaa aaggccacgc gactgtttct ttggaacaag
     3721 ggagacagat atgaacaaca tcaaaactgt aatcatcaca actctggcaa taccttttct
     3781 ggccggatca gggctgttcg ctcaggggta cgcatccgat atattcctgc cggactcgat
     3841 cttcgacttc ggcttttttg caactgatgc acgggttgtg catacatatc cgatagtgaa
     3901 tcgaggcaac gataccttga ggattacaaa ggtcaggccc ggatgcggat gtaccgttgc
     3961 ccctctggac aaatccaata tagctcccgg agacacggcc tacctggata tgtactttga
     4021 ttcaaagagg ctgacaggac tcgtgaaaaa gggaattaca atcttatcga a
//
LOCUS       DSEB01000416            5364 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001115, whole genome shotgun sequence.
ACCESSION   DSEB01000416 DSEB01000000
VERSION     DSEB01000416.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5364)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001115"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..551)
                     /locus_tag="ENO07_07525"
     CDS             complement(<1..551)
                     /locus_tag="ENO07_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HDS01856.1"
                     /translation="MPLIILAAAALVVLLQSAIKRSHGTALILTCLGLAAAFVSMFYA
                     ADELPRQVTALLIIDSYALFFTGLILFAAFAVAIFGYNYLNLYLENREEFYIVLLLAS
                     LGSSVLVSSNHFMSFFLGLEILTVSLYVMIGYMRKDHFSLEAGIKYLILAAASSAFLL
                     FGMALLYAETGTMEFNGIANAIGG"
     gene            complement(578..2140)
                     /locus_tag="ENO07_07530"
     CDS             complement(578..2140)
                     /locus_tag="ENO07_07530"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HDS01857.1"
                     /translation="MILVWLIVIPMAAGLLAWIFGKSSNLFARWISVLGIGIDLVISI
                     WLWIQHFAGVDPSRTESWLVQIKASWIPDLGITFHLAIDGISLLLIILTALLGLISVI
                     ISWTEIQRRVAFFHFNIMWILAGILGVFMAMDLFLFYFFWEMMLIPMYFLIGIWGHAN
                     RRYAAIKFFIFTQASGLLMLLSILGLYFIHHSQTGVYTYNYFDLLGTSLGGASRFLML
                     GFFVAFAVKLPAFPFHPWLADAHTEAPTAGSVILAGLLLKTGAYGIIRFVLPLFPEAS
                     MEIATLAMILGVISIIYGAILAFAQTDLKRLIAYTSVSHMGFVLLALFAFNEQATQGA
                     IMQMICHGLSTGALFILVGRMQELMHTRDMDRMGGMWQNVPRMSGVAMFFALASLGLP
                     GMGNFVGEFLILIGSFQANKVITIIAASGLVFAAIYSLWIIQRVFHGEQREEWKIPDL
                     RARFIGVFAVMIIILVVLGLYPRPVIDTAKPSIDTMQKNMEANMQIDLESSGTANLSS
                     DDWMIGVESERD"
     gene            complement(2137..4029)
                     /locus_tag="ENO07_07535"
     CDS             complement(2137..4029)
                     /locus_tag="ENO07_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HDS01858.1"
                     /translation="MLSLLWLVPAIPYFSFAILAVFGKRMPKSVSGLLGVGSVGISAV
                     ISVLIGIQFISSPPEGDLFLQRLWTWIGVGNFQPEIAFHLDALSLLMMLVVTIVGFLI
                     HLYSWEFMEDDEGFSRFFAYMNLFVGSMLTLVLANNLVLLYLGWEGVGLCSYLLIGFW
                     YKDSYNGAAARKAFITTRVGDTAMAIGLFLLFWNLGSLDIQEVMSSASMQWGTGSGIA
                     LAVALLLLGGAVGKSAQLPLQVWLPDAMAGPSPVSALIHAATMVTAGVYLIARTHVIF
                     DLAPIAQAAVAIVGAATLLIAGFSALKQHDIKRVLAYSTISQIGYMFLALGVGAYSAA
                     MFHFMTHAFFKALLFLGAGALIMCLHHEHNMFKMGGMRKQLPLTFWTFLIGSGALAAV
                     PLITAGFYSKDLILWRAWTSDSGSPILWLAGIVGAFITAVYTFRMVFVTFFGESKMQA
                     DRKPGVRISVPLVALAILALIGGFIEMPHTLGHFAPFSKFMHSVLPEVHAGQYSISAE
                     LLFQIIAVVITLSGILIAYIFYYRKPELSANVAKSGIGTVLGRFFYSGWGFDWLYYTF
                     LVGPFVWIARVNKKDIIDFIYTIIAMICQIFNFIFRQTQTGRVRSYAAGIAFGAIVII
                     AIVMFT"
     gene            complement(4030..4338)
                     /gene="nuoK"
                     /locus_tag="ENO07_07540"
     CDS             complement(4030..4338)
                     /gene="nuoK"
                     /locus_tag="ENO07_07540"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_856309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HDS01859.1"
                     /translation="MTAIPMEHGLLLAAVLFMLGLIGLLVRRNIIFMLLSIEVMLNAA
                     GLAFVVAGARWAQAEGQIMFMFILAMAAAEVAVGLALVLLMYRKFKTLDSDQVSRMRG
                     "
     gene            complement(4379..4873)
                     /locus_tag="ENO07_07545"
     CDS             complement(4379..4873)
                     /locus_tag="ENO07_07545"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HDS01860.1"
                     /translation="MAIIFYISSAVAVLSTVMVITRMNAVHALLYLIVSLLSVAVIFF
                     TLGAPFIGALEVIIYAGAIMVLFVFVMMMLNMGKKAAELESSWVNPRMYVGPAILAAI
                     LGIELVYMLASGSNRAAGFEMVGPKAVGIGLYSSYLVGLELAGMLLLAGLVGAFHLGR
                     RDED"
     gene            complement(4902..>5364)
                     /gene="nuoI"
                     /locus_tag="ENO07_07550"
     CDS             complement(4902..>5364)
                     /gene="nuoI"
                     /locus_tag="ENO07_07550"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HDS01861.1"
                     /translation="RETVQYPDEKVKLRPRYRGRIILSRDPDGDERCVACYLCSAACP
                     VDCISLQATEDEHGRRYPEFFRINFSRCIYCGLCEEACPTYAIQLTPDFEMSEPERPN
                     LVYEKEDLLISGEGKYPGYHFYKVAGLKIKDKDKGEAENEDEPIDFKKLLP"
BASE COUNT         1545 a         1494 c         1184 g         1141 t
ORIGIN      
        1 ccgccgatgg cattggcgat accgttaaat tccattgtac cggtttcggc ataaagcagg
       61 gccataccga ataacaaaaa ggccgatgag gccgccgcca gaattaaata ttttatgccg
      121 gcctcgagcg agaagtggtc tttcctcatg tagccaatca ttacataaag cgaaacagtc
      181 aaaatctcca gccccaggaa gaacgacatg aaatgattgc tggacaccag gactgatgac
      241 cccagagagg ccagcaacaa gacgatataa aactcctcgc gattttctaa ataaagattg
      301 agataattat atccgaatat agctaccgcg aatgccgcaa ataagatgag acccgtgaaa
      361 aacagagcat aagaatctat tattaatagc gcggtcacct ggcgcggaag ctcatcggcg
      421 gcatagaaca tactcacaaa agccgctgca aggccaaggc atgtaagtat caatgctgta
      481 ccgtggctgc gttttatagc tgactggagc agcactacaa gggccgctgc agccagaata
      541 atcaaaggca ttaatgctgt aaagtcctct gctgccacta atccctctcg ctttcaacgc
      601 cgatcatcca atcatcagac gataaatttg ccgttcccga tgactccaga tcaatctgca
      661 tatttgcttc catatttttc tgcatcgtat ctattgacgg tttggcggta tcgatcaccg
      721 gcctgggata caatcccagc acgaccagaa ttataatcat cacagcaaat acaccaataa
      781 atctcgctct caagtccggt atcttccatt cttcgcgctg ttctccatgg aatacacgct
      841 ggataatcca gagcgaatat atcgctgcaa atacaaggcc ggaggcggca atgatcgtga
      901 taaccttatt ggcctggaac gatcctatca gaatcagaaa ttcacccacg aaattgccca
      961 ttccgggcaa tcccaatgat gccagtgcga agaacatggc tactccggac atgcgcggaa
     1021 cattctgcca cattcccccc atccgatcca tatcacgggt atgcatcaat tcctgcattc
     1081 tccccaccag gataaacagc gcccctgtgc ttaaaccgtg acagatcatc tgcataatcg
     1141 ccccctgtgt cgcctgttcg ttaaatgcaa acagcgccag aagcacaaag cccatatggc
     1201 ttacgcttgt atatgcaatc agtcgtttca aatcagtttg ggcgaatgcc agaatcgcac
     1261 cgtagataat tgaaattact cccaaaatca tcgcaagcgt ggctatctcc atcgaggcct
     1321 cggggaacag cggcagtacg aatctgataa ttccgtaagc cccggttttg agaagcagcc
     1381 cggccaggat cacactccct gcggtgggcg cctcggtatg tgcatcggcc agccatggat
     1441 ggaacgggaa ggccggcagc ttaaccgcaa acgctacaaa gaagcccagc atcaggaacc
     1501 ttgaagcgcc gcccagcgat gtaccgagga ggtcaaaata attatatgta tagacgccgg
     1561 tttgactgtg atgaataaag tataatccca gaatagaaag cagcatcaga agcccgctgg
     1621 cctgtgtgaa aatgaaaaat ttaattgcgg catatcttct atttgcatgg ccccagatgc
     1681 cgatcaggaa atacatcggt atcagcatca tctcccagaa aaaataaaac aggaagaggt
     1741 ccatggccat aaacacaccc agtatcccgg ccagaatcca catgatattg aaatggaaga
     1801 aagccacccg cctttgaatc tcagtccatg agataattac cgaaatcagt cccagaaggg
     1861 cagtcaggat gatcaggagc agactgatac catcaatagc cagatggaat gttattccca
     1921 ggtcgggaat ccatgacgcc tttatctgca ccagccagct ttctgtccgg ctcggatcca
     1981 ctccggcaaa atgttgtatc cagagccata tcgagatgac cagatcaata ccgattccaa
     2041 gcaccgaaat ccatctggca aagagattac tgctctttcc aaatatccag gccaggagcc
     2101 cggcagccat cggtattact atcagccaaa caaggatcat gtgaacatca caatcgctat
     2161 aattactatc gcgccaaacg caatcccggc ggcgtaagac ctgacccgtc cggtttgtgt
     2221 ctggcggaaa ataaagttga atatctggca aatcattgca attattgtat atataaaatc
     2281 tataatatct ttcttgttaa ccctggcaat ccacacaaac ggcccgacca aaaatgtgta
     2341 gtagagccag tcaaaacccc accctgaata gaaaaatctc ccaagcacag taccgattcc
     2401 tgattttgca acgttcgctg aaagctcagg ctttctgtag tagaatatgt atgcaatcaa
     2461 aattccgctc agggttataa caaccgctat tatctggaat agcagctcgg cgctgatact
     2521 atactgcccg gcatgaacct cgggaagaac agaatgcatg aatttcgaga acggcgcgaa
     2581 atgtcccaat gtatgcggca tctcgataaa tccgccaatc aaggccagaa tcgccagcgc
     2641 caccaaagga acgctgatcc gtaccccggg cttacgatcc gcctgcattt ttgactcgcc
     2701 aaagaatgtc acgaaaacca tgcggaatgt gtatactgct gtaataaacg ctccaactat
     2761 tccggccagc cacaaaatcg ggctcccgct gtcggatgtc caggcgcgcc acaagattaa
     2821 atccttgctg tagaatccgg cggttatcag tggcaccgcg gcaagggcgc cgcttccgat
     2881 taagaaggtc cagaatgtca acggcagttg tttgcgcatc ccgcccatct tgaacatatt
     2941 gtgctcatga tgcagacaca tgattaatgc tcccgctcca agaaacagga gcgccttaaa
     3001 gaaagcatgg gtcatgaaat ggaacatggc tgccgaatac gcccccacac ccagagccag
     3061 gaacatgtag ccgatctggc tgatggtaga gtaggccagt acacgcttta tgtcatgctg
     3121 tttcaaagcg ctgaatccgg cgattaataa tgtcgccgca cccacaattg caaccgcggc
     3181 ctgtgcgatc ggcgcaagat cgaagatcac atgagtacgg gcaatcaaat acacaccggc
     3241 ggtcaccata gtcgcggcgt gaatcagggc gctgaccggc gagggaccgg ccatagcatc
     3301 cggaagccat acctgtaacg gcagctgggc cgattttcca actgctccgc ccagaagcag
     3361 gagcgcaact gcaagtgcaa ttccggagcc tgtcccccac tgcatcgagg cgctgctcat
     3421 tacttcctga atatcgaggc tgcccagatt ccagaatagc aggaacaagc cgattgccat
     3481 tgcagtatcg cccacacgtg tcgttatgaa tgcttttctt gccgccgcgc cgttgtagga
     3541 atccttgtac cagaaaccga tcaaaagata actgcacagg cccacacctt cccatccaag
     3601 atacagcagc acaaggttat ttgccagtac cagggtcagc atcgagccga caaacaggtt
     3661 catataggca aagaaacggc tgaagccctc atcatcttcc atgaactccc aggaataaag
     3721 atgaatcaag aagccgacaa tagttactac cagcatcatt aaaagcgaga gcgcgtccag
     3781 atgaaacgct atctcgggct ggaaattgcc gacgccgatc catgtccata gcctttgcag
     3841 gaataagtcc ccctcagggg gtgatgatat aaactgaata ccaatcagta cagatataac
     3901 agccgatatg ccgaccgagc ccacgcccag cagtccggaa actgacttcg gcatgcgctt
     3961 accgaatacg gccagaatgg caaagctgaa atatggaata gccggtacca gccacaatag
     4021 actcagcatc tatcccctca tcctgctcac ctgatcggaa tcaagcgtct tgaatttacg
     4081 gtacataagt aacaccagtg ccagcccaac cgccacctca gccgcagcca tagccaggat
     4141 gaacataaac atgatctggc cctcggcctg cgcccatctt gcgccggcca ccacaaaggc
     4201 caaaccggca gcattgagca taacctcaat cgagagcagc atgaagataa tattcctgcg
     4261 caccagaagg ccgatcaatc ccagcataaa cagtacggcc gccaaaagca gcccgtgttc
     4321 cataggaatt gcagtcataa tttctttgcc gctcctcttt ttatgtctac atcaaaaatc
     4381 aatcttcgtc cctgcgtccc agatgaaaag ctcccacgag tccggccaat aaaagcattc
     4441 ccgccagctc aagcccgact aggtatgagc tgtacaggcc gattccgacg gccttgggcc
     4501 cgaccatctc aaaaccggcg gcacggttgg aaccgcttgc cagcatataa accagttcaa
     4561 ttccaagaat agccgccaga atggcaggac ccacgtacat tctcggattg acccagctgc
     4621 tttccagttc cgcggccttt ttgcccatat tcagcatcat catcacgaac acaaacagca
     4681 ccataatcgc ccccgcatag atgattactt ccagggcgcc gataaaaggc gcacccaggg
     4741 tgaaaaagat caccgcgaca gaaagtaacg atacaatcag gtacagcaat gcgtgaacag
     4801 cattcatgcg cgttataacc atcacggtcg acaaaaccgc cactgccgat gagatgtaga
     4861 aaattattgc cataagttaa accgccatct gcctatctta ctcaaggcag caacttcttg
     4921 aagtcaatcg gttcatcctc attttccgcc tcgcccttgt ccttatcttt gattttaagc
     4981 ccggcaacct tgtaaaagtg ataaccggga tattttccct cacctgaaat cagtaagtcc
     5041 tctttttcat agaccagatt gggacgctct ggttctgaca tctcgaaatc cggggtcaac
     5101 tggatcgcat aggtcgggca ggcctcctcg cacagtccgc aataaatgca cctggaaaaa
     5161 tttatccgga aaaattcggg atagcgccgg ccatgttcgt cctcggtcgc ctgcagtgag
     5221 atacaatcca ccgggcaggc cgccgaacac agatagcagg ccacacatct ttcatcgccg
     5281 tcaggatcgc gcgatagaat aattcgtcca cgataccgcg gacgcagctt taccttttca
     5341 tccgggtact gtacagtctc acgc
//
LOCUS       DSEB01000417            5365 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001114, whole genome shotgun sequence.
ACCESSION   DSEB01000417 DSEB01000000
VERSION     DSEB01000417.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5365)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001114"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..255
                     /locus_tag="ENO07_07555"
     CDS             <1..255
                     /locus_tag="ENO07_07555"
                     /inference="COORDINATES: protein motif:HMM:PF00430.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01862.1"
                     /translation="IQEAVKEGQQVATEIKDSARKESRELIEKAKSELQRDVEKAKVQ
                     LKEDLVSMTIGATEKLILERLDDQKHRQLITNFIDDVEKA"
     gene            252..803
                     /gene="atpH"
                     /locus_tag="ENO07_07560"
     CDS             252..803
                     /gene="atpH"
                     /locus_tag="ENO07_07560"
                     /inference="COORDINATES: protein motif:HMM:PF00213.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HDS01863.1"
                     /translation="MVISSEVAKRYAVGLFELALEKNMIDTIAEEMRSIAEVCKQDKT
                     LLNFLTAPQIRDQDKEEVIKAVFADRVSPPVKRFIDFIIEKRRSKYMIEIAEAFNDLV
                     LESQGFLKTRVETAIELTEEETRALQKKLEQKTGKKILMSKFVRPQILGGMIVYLGSQ
                     IIDKSIRHDLQILRDRLLDVKVH"
     gene            869..2398
                     /locus_tag="ENO07_07565"
     CDS             869..2398
                     /locus_tag="ENO07_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012303201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDS01864.1"
                     /translation="MGLKPEEVSSIIRQEISKYETTLKMESVGTILQIGDGIARVWGL
                     EDAMMSELVMFPGDIMGLILNLEEDNVGVAIFGSDTNIKEGDTVKRTGRIASVPVGDA
                     LVGRVVNALGQPIDGKGPIVTDKYRPTEVVPPNVVQRQPVKEPVQTGLKAIDSMIPIG
                     RGQRELIIGDRQTGKTAVAIDTIINQKNSDIYCIYVAIGQKSSTVAQIVDILERHGAM
                     EYTTVISAPATDPAPLQYLAPYSGCAMGEEFMQTQRHALVVYDDLSKHANAYRQLSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDELGGGSLTALPVIETQAGDVSAYIPTNV
                     ISITDGQIFLEGELFYSGIRPAINVGISVSRVGGNAQTKAMKKVAGRLRLDLAQYREL
                     AAFAQFGSDLDKATLRQLTRGEKMVEILKQGQYVPMPMEQQVMIIWAGGQGYMDDVPT
                     DLVSNFEQEYLKFMESDYPDVGHKIAQDKIISEENEKQLRAGTEAFKEKFMAEKGLSK
                     G"
     gene            2402..3277
                     /gene="atpG"
                     /locus_tag="ENO07_07570"
     CDS             2402..3277
                     /gene="atpG"
                     /locus_tag="ENO07_07570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HDS01865.1"
                     /translation="MLSLRDIRKRIRAVESTKQITKTMEMVAAARLRKAQQQIESFRP
                     YARKMQEMLAHLSEASGAVEHPYFEQREGNKVALVVISSDRGLCGSYNSNIFRRTNLW
                     VADHPDLDVGLILIGKKAINFYKRRDYKILGSYRDFDGKFDIGKVRQITNDLSEMFIS
                     GDVDRIVFIYTAFLSMASFRITETQFLPIRAEFEEAVEKEEYAAAKEYIFEPDPETIF
                     KQLLPSYALVLVQMAVADAIASEHGTRMLAMGSATKNAGEMIEQLTLQRNKARQASIT
                     SELLEIVSGAEALNK"
     gene            3372..4784
                     /gene="atpD"
                     /locus_tag="ENO07_07575"
     CDS             3372..4784
                     /gene="atpD"
                     /locus_tag="ENO07_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019542567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDS01866.1"
                     /translation="MAEENVGKVLQVIGATVDCQFDSDKLPNILNAIKIVDKEREINL
                     TVEVALHIGDNMVRCISMASTDGLVRGMKAIDTGEPIAVPVGEQVLGRVFDLEGHPID
                     ELAPIPEGTETRPIHHPPPSFTDQDIETSIFETGIKVIDLLEPYAKGGKVGLFGGAGV
                     GKTVIIMELIRSIASEHAGYSVFCGVGERTREGNDLWLEMKESGVIDKTCMVFGQMNQ
                     PPGARLRVGLSGLTMAEYFRDEMHQDVLLFIDNIFRFVQAGSEVSALLGRMPSAVGYQ
                     PTLSNEMGALQERITSTKAGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRQIA
                     ELGIYPAVDPLDSTSRILDPNVVGEEHYKVARDVQMILQRYKDLQDIIAILGIDELSE
                     EDKLTVARARKIQRFLSQPFFVAEQFTGIPGKYVPLSETIASFKELVEGKCDDMPEQA
                     FYMVGNIEEARERAEEMKKD"
     gene            4788..5201
                     /gene="atpC"
                     /locus_tag="ENO07_07580"
     CDS             4788..5201
                     /gene="atpC"
                     /locus_tag="ENO07_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HDS01867.1"
                     /translation="MFKLSIVSPERVLYEDDVESLVAPGIDGYLGILSNHAPLITSLV
                     TGRIEIRDAHKIEKIAAISQGFLEVSGNTATILADAVEFVEEIDLSRAESALKRAEER
                     VNMDSGDIDLPRARNALIRARNRIKLVKQFKQMKK"
BASE COUNT         1377 a         1210 c         1510 g         1268 t
ORIGIN      
        1 attcaggagg cggtgaaaga gggccagcaa gttgcgaccg aaatcaagga ttccgcccga
       61 aaggaatcac gggaactgat tgaaaaggcc aaatcggagc tgcagcgcga tgtggagaag
      121 gccaaggtcc agttgaagga ggacctggtc agcatgacca tcggtgcaac cgaaaaactg
      181 atcctggagc ggctcgacga ccagaaacat cgccagttga tcacaaattt cattgatgat
      241 gtggagaaag catagtgatt tcctcagagg ttgccaagag gtatgctgtc ggcctttttg
      301 agctggcgct ggaaaagaat atgatcgata cgatcgcgga agagatgcgc tctatcgccg
      361 aagtgtgcaa gcaggacaaa acgcttttga attttctgac tgctccccag ataagggatc
      421 aggataagga agaggttata aaggccgtct ttgctgatcg tgtgtctccg ccggtgaaaa
      481 gatttatcga ttttatcatt gaaaagcgca ggagcaagta catgatcgaa atcgctgagg
      541 ctttcaatga tctggtcctg gagagccagg gcttcctgaa aacccgtgtc gaaacagcaa
      601 tcgaactgac cgaggaagag accagggctt tgcagaagaa actggagcaa aagaccggga
      661 aaaagattct aatgagcaaa tttgtcaggc cccagattct gggcgggatg atcgtatacc
      721 tcggaagcca gattatcgac aaatcgattc ggcatgatct tcagatcctg cgcgacaggc
      781 tgctggacgt gaaagtgcat tgatattatc ggcgttttga tataaagatt ttttgaaaac
      841 gattttcttg atatagacgg agattaatat gggattaaaa ccagaagaag taagctccat
      901 aatcaggcag gagatctcca aatacgaaac cactctcaag atggagtcgg tgggtacgat
      961 cctccagatt ggtgacggga tcgctcgtgt ttggggcctt gaggacgcta tgatgtcgga
     1021 gcttgttatg ttccccggcg atattatggg actgatcctg aaccttgagg aggataatgt
     1081 cggtgtcgct atcttcggtt ccgatacgaa tataaaagaa ggcgatacag tcaagcgtac
     1141 cggcagaatc gcatcagtcc cggtgggaga cgccctggtg gggcgcgttg tcaacgctct
     1201 cggtcagccg atcgacggca agggtccgat cgtaaccgat aagtatcgtc cgacagaagt
     1261 tgttcccccc aacgtggttc agaggcagcc ggtgaaagag ccggtacaga caggcctgaa
     1321 ggcaattgac tcgatgattc cgatcggaag agggcagcgt gagctgatca tcggcgaccg
     1381 ccagacaggc aagaccgccg ttgccatcga tacgattatc aatcagaaaa attccgatat
     1441 ttactgtatc tatgtggcga tcggtcaaaa gtcctcgact gttgcgcaga ttgtggatat
     1501 tctggaaagg cacggggcca tggaatatac aaccgtaatt tccgcgcccg ccaccgatcc
     1561 tgcaccattg cagtatctgg ccccctattc cggatgcgct atgggcgagg agttcatgca
     1621 gacccagcgt catgctctgg tggtttacga cgatttgtcc aagcatgcca atgcttatcg
     1681 tcagctctca ctgcttttga ggcgtcctcc gggacgtgag gcttatccgg gtgacgtgtt
     1741 ctatctgcat tcgcgtcttc tggaacgtgc cgccaaatta aatgatgaac tgggcggcgg
     1801 atcgcttacc gccctgccgg ttattgagac ccaggcaggg gacgtgtcgg cctatattcc
     1861 gaccaatgtt atttcgatta cagacggcca gattttctta gagggagagc tgttctattc
     1921 gggtattcgt cccgccatca acgtgggtat ttcagtctcg cgtgtgggcg gcaacgctca
     1981 gaccaaggcc atgaagaagg tcgcgggaag gctgcgtctg gacctggctc agtatcgtga
     2041 gctggccgct tttgcacaat ttggatctga tctggataag gccactttga ggcaactgac
     2101 gcgtggtgaa aagatggttg aaattttgaa gcaggggcag tatgtgccta tgccgatgga
     2161 gcagcaggtt atgatcatct gggccggcgg tcaaggctac atggatgatg tccccaccga
     2221 tttggtttcc aatttcgaac aggaatatct taagtttatg gaatctgatt atcctgatgt
     2281 gggccataag attgctcagg ataagataat ctccgaggag aatgagaaac aactgcgggc
     2341 cggtaccgag gccttcaaag agaaattcat ggccgagaag ggattatcaa agggataatt
     2401 aatgctttca ctgagagata tcagaaaacg aatccgtgct gttgaatcga ccaagcagat
     2461 aaccaagact atggagatgg ttgctgccgc cagactcagg aaagcccagc agcagatcga
     2521 atcgttccgt ccctatgcca gaaagatgca ggagatgctg gcccatctat ccgaagcttc
     2581 cggggccgtg gagcatccct atttcgagca gcgggaaggg aataaggttg cgctggttgt
     2641 tatttcatcc gacaggggcc tgtgcggatc atataacagc aatatcttca ggcgcaccaa
     2701 tctgtgggtt gccgatcatc ccgacctgga tgtcggccta atcctgatcg gcaagaaggc
     2761 gattaatttt tacaagcgcc gtgattacaa gatactgggc agttacaggg atttcgatgg
     2821 aaaattcgat atcggcaaag tccggcagat taccaatgat ctctcggaga tgtttatctc
     2881 cggcgatgtt gatcgtatcg tctttatcta tactgccttt ttatcgatgg cctcattccg
     2941 gataactgag acgcagtttt tgccgatcag ggctgagttc gaggaggctg ttgaaaaaga
     3001 ggagtatgct gcggcaaagg aatatatctt cgagcccgat ccggagacaa tattcaagca
     3061 gctgcttccc agctatgcgc tggtactggt gcagatggcg gttgccgatg cgatagcatc
     3121 cgagcacggc acgcgtatgc tggccatggg ttcggccacc aagaatgcgg gagagatgat
     3181 tgagcagctg accctgcagc gtaacaaggc ccggcaggcc agtattacaa gtgaattgct
     3241 ggaaatcgta tccggtgccg aagcgcttaa caagtagcac ggttttaaat tgaataaacg
     3301 caacccattt gtgggtactt tgcatataga tttgagcttg gaaaaaaaca ataattaata
     3361 aaggaattgc aatggcagaa gagaatgttg gtaaagtgct acaggttatc ggggcaactg
     3421 ttgattgcca gtttgattcc gataaattac ctaatattct taatgcgatc aagatcgtag
     3481 ataaagaaag agagatcaat ctgaccgtgg aggtagcctt gcatatcggc gacaacatgg
     3541 tcaggtgtat ttctatggca tctacagatg gtctggtgcg cggcatgaaa gctatcgata
     3601 ccggcgaacc gattgccgtt ccggtgggcg agcaggtgct tggacgagta tttgacctgg
     3661 aaggtcatcc catcgatgag ctggctccca tcccggaagg caccgagacc aggccgatcc
     3721 atcacccgcc cccgtcattc acagaccagg atatcgagac ctcgattttc gagaccggta
     3781 tcaaggttat cgatcttctt gagccctacg ccaaaggcgg aaaggttgga ctgttcggcg
     3841 gtgcaggtgt tggcaagaca gttatcatta tggagctgat ccgcagtatc gcttccgagc
     3901 atgccggtta ttcggtgttc tgcggtgtgg gcgaaagaac ccgtgagggt aatgacctat
     3961 ggctggaaat gaaggaatcc ggtgttatcg acaagacctg catggttttt ggccagatga
     4021 accagccgcc gggagcccgt ttgagagtgg gactttccgg gctaaccatg gccgagtatt
     4081 tccgtgatga gatgcaccag gacgtgctcc tgttcattga taatattttc cgttttgtgc
     4141 aggccgggtc tgaagtgtcg gcgcttctgg gacggatgcc ctcggcggta ggttaccagc
     4201 ctacgctctc caacgaaatg ggcgccctgc aggagcgaat cacatccacc aaggcaggtt
     4261 caattacgtc ggtgcaggcg atttacgttc cggctgacga tttgaccgat ccggctccgg
     4321 caacaacctt tgcgcacctt gacgccacca cagtgctgtc gcgccagatc gcagagctcg
     4381 gtatttatcc ggcggtcgac ccgctcgact ccacctcaag gattctggat ccgaatgtgg
     4441 ttggtgaaga acactataag gtcgcccgtg atgtgcagat gatcctccag agatacaagg
     4501 acctgcagga tattatcgct attctgggta tcgacgagct atctgaagag gataagctga
     4561 ctgtggccag ggccaggaaa atccagagat tcctctcgca gcccttcttt gtggccgagc
     4621 agtttaccgg aattcccgga aaatatgtgc ctctgtcaga gaccatagcc agcttcaagg
     4681 agctggtaga gggtaaatgt gatgatatgc ctgagcaggc tttctacatg gtaggaaata
     4741 ttgaagaggc tcgcgagcgg gccgaggaaa tgaagaaaga ttaacctatg tttaagctgt
     4801 ctatagtatc gcctgaacgg gttttgtacg aggatgatgt cgaatccctg gttgcccccg
     4861 ggattgacgg ctatcttgga atactctcca atcatgctcc gctgataaca tcgctggtga
     4921 cggggaggat cgagatacgc gacgcccaca agatagagaa gattgcggcc atctcacagg
     4981 gcttcctgga agtctccggc aacaccgcaa ccattcttgc tgacgcggtt gagtttgtgg
     5041 aggagatcga tctttccaga gcggaaagcg ccctgaagcg tgctgaagag agagtcaata
     5101 tggattccgg tgatatagat ctgccgcggg cacgcaacgc attaatcagg gccaggaaca
     5161 gaataaagct ggttaaacag tttaagcaga tgaaaaaata aagtaaaaaa cccgttcatc
     5221 cgaacgggtt ttttactgtg cttagagtag ggttgtgcag ggggttgggc tatatttgta
     5281 aagagcaggt ttctttttat tgacctccct gagaaatact catccacttg gaaaagtggg
     5341 acatcttaat atagactttg tctcc
//
LOCUS       DSEB01000418            2885 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004476, whole genome shotgun sequence.
ACCESSION   DSEB01000418 DSEB01000000
VERSION     DSEB01000418.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004476"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            311..2062
                     /locus_tag="ENO07_07585"
     CDS             311..2062
                     /locus_tag="ENO07_07585"
                     /inference="COORDINATES: protein motif:HMM:PF05598.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01868.1"
                     /translation="MFHVKDHKTDYLFEPFGYMGSKRLKRIKESWAGLFREEILSELP
                     VEELTGHYDPTQGRPSKEVYAVLGAVLLQQMMDLTDEETIDNFAFNLQWHYALNVTDE
                     SDKGSYLSPKTLWTMRTMIMRDGLAPVIFDRVAEKLKVIFDVNTDQQRLDSMHIFSNM
                     RHLGRVGLIAQTLRKFMVNLKRQDREEFLALPEEVRECYMPKRGEAVFAMVKPSEAQR
                     ALQTLGKDMFELIERFRQHEKIREMNSYGLLVRVFGEQFQVETSDGESLILINRKVSC
                     DSLQNPSDPDATYDGHKGKGYQVQVLESYSAEKDDNKEAPLLNLITYVEAQPAHEHDA
                     HTVKKALEKTRRENRAPRQILADSLYGGDNNHVDCQAEGVDLIAPVKTNKTCPNVGLA
                     NFSYGAQNRITACPEGHAPISSQHRKDRHSAWFDSDLCRKCPLREECPVKPGGKGHFI
                     RYSNKELRLELRKADEQTPEFREQYRYRAGCEATMSELDRKTGIKHLRVRGMSAVSLC
                     VYLKATGINIFRAARAWKRPLLPKAVPSRASLRLKRGLDNGLIAFRSVYMSLRRQIGG
                     LNAIFAPTLRFSPNFAI"
     gene            2102..>2885
                     /locus_tag="ENO07_07590"
     CDS             2102..>2885
                     /locus_tag="ENO07_07590"
                     /inference="COORDINATES: protein motif:HMM:PF01266.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HDS01869.1"
                     /translation="MNRAETLARLESQNEWDLLVIGGGATGLGICLDAASRGYKTLLV
                     EQSDFAKATSSRSTKLIHGGVRYLEQGNITLVADALKERGRLIKNAPELVKPLRIVIP
                     LYHWWQVPYFGTGLKIYDHLSGRFSLGRSRIMGREKILREIPALKAEKLSGGVLFYDG
                     QFDDSALAVSLALHSSREGAAVLNYTRFTAFTREEGKIQGGIVEDVLTGRQYKVRSRA
                     VINAAGVFGDEIRQMDRPDTPDMIVPSQGAHIVLDQKFLPGDI"
BASE COUNT          763 a          621 c          803 g          698 t
ORIGIN      
        1 attgagggct gttgccctca atcaattgaa gattcaagat tcaagattca agattcaaaa
       61 ctgactggga ttctgctcat gatattagct caggctatta aatgtcatgc tttcagcctt
      121 cacccggttg ccgcctgacg gaggatataa tttaattgcc aatcttgaat cttgaatctt
      181 gatggactcg taaaaagtct atttcatgta aatatttaat agtattaaga aaaatgtttt
      241 tggcggctca taaatgtcag ggttttgtga tagtttattc aatattatca ataatttaaa
      301 tggagaataa atgtttcacg tcaaagatca caaaaccgac tacttgtttg aaccatttgg
      361 atatatgggc tccaagcgtc tgaagaggat caaagaatcc tgggcagggc tttttcggga
      421 ggagattctg tcggaacttc ctgttgaaga attgacaggg cattacgatc ctacccaggg
      481 ccggccgagc aaagaagttt atgcagtgct gggagcggtg ttattgcagc agatgatgga
      541 tctgaccgac gaggagacga ttgacaattt tgccttcaat ctgcaatggc actacgcatt
      601 gaatgtgacg gatgaatcgg ataaaggatc ctatctgagc ccgaagacac tgtggacgat
      661 gcggacaatg ataatgaggg acgggttggc tccagtgata tttgatcgag tcgccgagaa
      721 gctcaaggtg atctttgatg tcaataccga tcagcagcgt cttgactcga tgcatatctt
      781 ctcgaatatg cggcatcttg gccgtgtcgg actgattgcg cagacgctgc gaaagtttat
      841 ggtgaacctg aagcgtcagg atagagagga attcttggct ttacccgagg aggttcggga
      901 gtgttatatg ccgaagcgag gagaagcggt ttttgccatg gtcaagccgt cggaagccca
      961 gagggccttg caaacgctgg ggaaggatat gtttgaactg atcgaacggt tcagacagca
     1021 tgaaaagatc cgcgagatga acagttacgg attgctggta cgggtttttg gggagcagtt
     1081 tcaggtggaa acaagcgacg gggaatccct gattttgata aacaggaagg tatcttgcga
     1141 ctcactgcag aacccttcgg accctgatgc cacctacgat ggacacaagg gcaaaggcta
     1201 tcaggtgcag gttctggaga gttattcagc agagaaagat gataacaagg aagcgccgct
     1261 tttgaacctg ataacctacg tggaagcgca accagcgcat gagcatgacg ctcatacagt
     1321 gaaaaaggct ttagaaaaga cccgtcggga aaaccgggcc cccaggcaaa ttctggctga
     1381 cagcctgtat ggcggagata acaaccatgt cgattgccag gctgagggcg tggacctgat
     1441 tgctccggtg aagaccaata agacttgtcc gaatgttggc ttggccaact tcagttacgg
     1501 ggcacagaat cggatcactg cgtgtccaga ggggcacgct ccgataagca gccaacatcg
     1561 caaggatcgt cacagcgcat ggtttgattc ggatctgtgc cggaaatgtc ctctccggga
     1621 ggaatgtccg gttaagccag gcggtaaggg gcattttata cgttacagca acaaggagct
     1681 gcgtttggag ctgcgcaaag ccgatgagca gacaccggag tttcgggagc aataccggta
     1741 tcgagctgga tgcgaagcaa ccatgtcgga gttggatcgc aaaactggta ttaagcattt
     1801 gcgagttcga ggtatgtcgg cggtgagctt gtgtgtctat ctgaaggcaa ccggaatcaa
     1861 tatctttcga gctgccaggg cctggaaacg accgctttta ccaaaggctg ttccctccag
     1921 ggcctctctg aggcttaaaa gaggtctgga taacggcctg atcgctttta ggtctgttta
     1981 catgtctctt cgaagacaaa ttggagggct gaacgctatt tttgctccca ctttgcgatt
     2041 cagccccaat tttgctattt aagtttttac gagtccatca atcttgaatc ttgaattaaa
     2101 tatgaacaga gctgaaacat tagcgagact tgaatcacag aacgaatggg atctgctggt
     2161 aatcggaggc ggagccaccg ggcttgggat ctgcctggat gctgcatcca ggggctacaa
     2221 gaccctgctt gttgagcaat ctgattttgc caaggctact tccagccgca gtacgaagct
     2281 tattcatggg ggagtgcgct acctggaaca gggaaacatt accctggtgg ctgatgccct
     2341 aaaggaaagg ggccgcctga ttaaaaacgc cccggaactg gtaaaacccc tgcgaatcgt
     2401 gattcctctt tatcactggt ggcaggtccc ctactttgga accggcctga aaatttatga
     2461 ccatctctcc ggcaggttca gccttgggcg atccagaata atgggaaggg aaaagatcct
     2521 gagggagatt ccggccctga aagcagagaa actgagcggc ggggtactat tctatgacgg
     2581 ccagtttgat gactccgccc tggctgtttc cctggcgctg cattcttccc gggaaggtgc
     2641 cgccgttctt aactacacca ggttcacagc gttcaccagg gaagaaggta agattcaggg
     2701 aggcattgtg gaagacgtgc tgactggaag acagtataaa gtcaggagcc gggctgttat
     2761 aaacgcggcc ggagttttcg gagatgaaat aaggcagatg gacaggccgg atactcctga
     2821 catgatagta cccagccagg gagcccatat tgtgctggac cagaaattcc tgcctggtga
     2881 tatag
//
LOCUS       DSEB01000419            3515 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002898, whole genome shotgun sequence.
ACCESSION   DSEB01000419 DSEB01000000
VERSION     DSEB01000419.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002898"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(397..1791)
                     /locus_tag="ENO07_07595"
     CDS             complement(397..1791)
                     /locus_tag="ENO07_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HDS01870.1"
                     /translation="MKFDLNKIYQHMPVFIQNLGVSYYGLKLYRREYGKKFRAMLEEF
                     EKQQWYSYDELREYQNRLLRKLIKHCYDNVPYYNQVMRGRKLKPEDIKGVDDLKKMPL
                     LTRETIKEHGDELLAGNVNQKKLVIGYTSGTIGVPMKLVWDKRVCLVKNVVDWRQKSI
                     AGIKPGDRMAFLYASAVVPLNRRKPPFWRYNWILNHLHFSNYHLLDNVEYYVNKLSAF
                     KPKAIEGYPSLLYVLARFMQSENIVIPVQAVFTSSETLMPHQREALEAVFDCKVFDFY
                     GLAERDVFATECEFHRGKHLNEDFGITEVMVDGERPTKAGEMGRIVATGLHNYAMPLI
                     RYQTTDVTMLASSECACGRSFPLMENVTARDQDFLVTADGRRISPYIVVGITRRFTNI
                     REHQFIQEDGNSLRILIIRSDSYTDEDSRALIEQMKNVFGEKMNIELEFVSSIPRTKS
                     GKFRAVISKISAEF"
     gene            complement(1788..2732)
                     /locus_tag="ENO07_07600"
     CDS             complement(1788..2732)
                     /locus_tag="ENO07_07600"
                     /inference="COORDINATES: protein motif:HMM:PF13641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDS01871.1"
                     /translation="MKISFIIPVLNGEKYIRQCLDHIAAEMHPDDEIIVVDNGSTDST
                     LDIVRQYEAVKILVHPGITIAALRNRGAEVAAGDLLAFIDSDCLVCKGWRKAVEEAFT
                     REDVHAAGSSYMVRENPTWIEAAWCQQVKRKISRFYFVPSGNFVIRNDVFRDIKGFNE
                     NFETDEDTEICRRVNQHGYSILNDPDICVVHLGNPRTMREFIGKERWHASAITKPPSA
                     KKMDKPLIMTYLFMLFLLFTIISLFMIPVWGWIFPVIGVILFLLVPVFTAFYKSYSIG
                     RISYVPSLIILFLVFYLVRSEVIVKSIFTKNLSKKDPG"
     gene            complement(2811..>3515)
                     /locus_tag="ENO07_07605"
     CDS             complement(2811..>3515)
                     /locus_tag="ENO07_07605"
                     /inference="COORDINATES: protein motif:HMM:PF00534.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDS01872.1"
                     /translation="FDRIHVVSDDIKDSLEEKFGADKLVTIHNVVDEEFFGAVPDRDK
                     IREELGIDEGAMVLGTAGRMVPVKGYKMLIQAARIVVDNNSGVKFLLAGDGPLKLELE
                     RKADDMRLAGDVKFVGFRNDMIDFLGALDIFVMTSYHEGIPVVLLEAMALGRPTVATA
                     VGGIREVIEAGKSGILTKPSDHIEFAETCQRLLQDVELRRSLGEGAKERIRSAFSSTV
                     QREKIHKLYSELMVGQ"
BASE COUNT          950 a          919 c          683 g          963 t
ORIGIN      
        1 tattattatc cgagccgtta ttcgacacat accatgaatg atcgttttga gccagggctt
       61 gggcgggaag gaaagtcagc gccagaagca atccaagaaa gaaaaattta tagctcgcag
      121 aaatgggctt tataatcctc gacattactc atctcctcgc atgaagtcaa atgggccatt
      181 caaataacag attcaatttg aaggccaaaa caacatcttt ttttgaatac ctaagtcctt
      241 acaaatcttt acagtatcaa ttgaagaatc ggcagatttc ccctaatatt taaaccaggt
      301 tcttttttca ttcaaaatgt tcaatttttg cacttcctaa gtcgtttatt tttgtacgaa
      361 atttatatca attatacaag ttaaaatttc ggtagatcag aattcagcgg aaattttaga
      421 tatcacagcc cgaaatttcc ctgattttgt cctgggaatg ctggacacga attccagttc
      481 gatgttcatc ttctcgccaa agacattctt catctgctct attaaagccc tcgaatcttc
      541 gtccgtatag ctgtctgacc tgataatcag tatgcgcagc gaattcccat cttcctgaat
      601 gaactgatgt tcccggatat tggtaaaccg acgtgttatg cccactacaa tatagggcga
      661 tatgcgccgc ccgtctgcag tcaccaaaaa atcctgatct cttgcggtaa cattttccat
      721 aagaggaaaa ctgcggccgc aggcacattc tgaggaagcc agcattgtaa catcagttgt
      781 ctggtaacgg attaacggca ttgcgtaatt atgtaatcct gtcgcaacta ttcttcccat
      841 ctcacccgcc tttgtcggcc gctcaccatc aaccataact tccgtgatac cgaaatcctc
      901 gttaagatgc ttaccccgat gaaattcgca ttctgttgca aacacatccc gctcggcaag
      961 accatagaag tcaaagactt tgcagtcgaa gacggcttca agggcctccc tctgatgagg
     1021 cataagggtc tctgaggagg taaagacggc ctggactgga atcacaatgt tctcggattg
     1081 catgaaacgg gcgagtacat acaataagga tgggtagccc tctatggcct ttggtttgaa
     1141 agccgatagt ttattgacat aatattccac attgtccagc aggtggtagt tcgagaaatg
     1201 caggtgattc aatatccagt tatacctcca gaatggcggc tttcttctgt ttagaggaac
     1261 aactgcgctt gcatagagga aagccatcct gtcgcccggt tttatacccg cgatgctttt
     1321 ctgcctccag tcaaccacgt tcttgaccag gcataccctt ttatcccata caagcttcat
     1381 cgggacacct atcgttccgc tggtgtatcc gattacgagc ttcttttgat taacattccc
     1441 ggccagaagc tcatccccgt gctctttaat ggtctctctt gtcaaaagcg gcatcttctt
     1501 taaatcatcg acacccttga tatcctcagg ttttaatttt cttccgcgca tgacctgatt
     1561 gtaatagggc acattatcat aacaatgctt gatcaacttc ctcagcaacc gattctggta
     1621 ctccctgagc tcatcatatg aataccattg ctgcttctcg aattcctcca gcatggccct
     1681 gaatttcttt ccatattcgc gccggtagag cttcaggccg tagtagctaa cgcccagatt
     1741 ctgaatgaaa acgggcatat gctgatatat cttattcaaa tcgaatttca tcccggatcc
     1801 tttttactta gattttttgt gaatatcgac ttgacgatta cctcggaacg aactaaatag
     1861 aaaaccagaa atagaataat caagctcgga acataagaaa tacgcccgat tgaatagctt
     1921 ttataaaatg cagtgaaaac gggtactaat aaaaacagga taacaccaat caccggaaag
     1981 atccatcccc agacaggaat catgaataac gatattattg tgaagagaag gaaaagcata
     2041 aataagtaag tcattatcag cggcttatcc atttttttgg ccgaaggtgg cttggtgatt
     2101 gctgaggcat gccagcgttc tttcccgatg aattcccgca ttgtgcgcgg atttccgaga
     2161 tgaaccacgc agatatccgg atcattcagg atagaataac cgtgttgatt gacccgcctg
     2221 caaatctcag tatcctcatc tgtctcaaaa ttctcattga aaccttttat atcccgaaac
     2281 acatcatttc taataacaaa attcccgctt ggaacaaagt agaatctgct gatcttcctt
     2341 tttacctgtt gacaccaggc ggcctcgatc caggttggat tttctctcac catataggat
     2401 gaccccgcgg catgaacatc ctcccgggtg aacgcctctt caactgcttt cctccagccc
     2461 ttgcagacca gacaatccga atctataaag gccagcaggt ctcctgcggc aacttcggcg
     2521 ccgcggtttc tgagggccgc gatggttatg ccgggatgaa ccagtatttt caccgcttcg
     2581 tactgtctga caatatcaag cgtgctgtcg gtggaaccgt tatcgaccac gatgatctca
     2641 tcatcgggat gcatctcggc tgcgatatga tcaaggcact gccttatata tttctcaccg
     2701 ttcagaaccg gaattataaa acttatcttc atatacaact catcacattc ttgatactgc
     2761 tagcacaggc agtttgactg cttccgagat cgcccccgaa acaacaacca ttactgcccg
     2821 accatcagtt cactatacag cttatgaatc ttctccctct gcacggtaga gctgaaagcg
     2881 gatctgatac gctcttttgc cccctcgccc aggcttctcc tgagttcaac atcctgcaaa
     2941 agtctctggc aggtctcggc aaactcaata tgatccgagg gctttgtcag aattcccgat
     3001 ttgccggcct caatcacctc ccgaatccct ccgactgcgg tcgccactgt cggccgtccc
     3061 agcgccatag cctccagcag gacaaccggt atgccctcat gatacgaagt cataacaaat
     3121 atatcaagag cgcccagaaa atcaatcata tcattcctga agccaacgaa tttgacatcc
     3181 ccggccaacc tcatatcatc ggccttacgc tccaattcca acttcaaagg gccgtcacct
     3241 gccagaagaa atttgacgcc ggaattatta tccacaacta ttcgggccgc ctggatcagc
     3301 atcttgtatc ctttcacagg aaccatccgg ccggcagtgc cgagcaccat agcaccctca
     3361 tcaattccca gctcttcccg aattttgtcc ctgtccggaa ctgcaccgaa aaactcttca
     3421 tcgacgacat tgtgtatcgt aacaagctta tcagctccaa atttttcctc gaggctgtct
     3481 ttaatatcgt cggagacgac atgtatccgg tcgaa
//
LOCUS       DSEB01000420            1648 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1015501, whole genome shotgun sequence.
ACCESSION   DSEB01000420 DSEB01000000
VERSION     DSEB01000420.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1015501"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            1..456
                     /locus_tag="ENO07_07610"
     CDS             1..456
                     /locus_tag="ENO07_07610"
                     /inference="COORDINATES: protein motif:HMM:PF01464.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDS01873.1"
                     /translation="MLRGAGLPEEFAYLAFVESGYDSTATSPAGARGLWQFMPATARD
                     FGLTVNNDMDERVNAKKSTEAACKYIKQLYNLYNSYMLAMASYNTGLGRIRNALMRMD
                     VIEQNRFWYLVKNDMLHNETVEYVPKIMAAMIIATDPVKFGFSEAPEKK"
     gene            453..>1648
                     /locus_tag="ENO07_07615"
     CDS             453..>1648
                     /locus_tag="ENO07_07615"
                     /inference="COORDINATES: protein motif:HMM:PF00069.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HDS01874.1"
                     /translation="MSARRIIDEQYEIIREIKAGGFGTIYYGWDLTLDRPVAIKEVVK
                     SLLGEKQYVDMFIDEAVNTARLNHPNIVQVYSLRKTPDDRVFIIMQFIEGVDLREVID
                     YYNDRNETMPKNMAMHIISEICKALEYAHNLKDRKSGQPLNIVHRDISPSNIMLTVEG
                     SVKLIDFGIAKARNRIVQKTQTGFVKGKVAYMSPEQLEGKEATRQSDIFSLGSVFYEM
                     CTGEELFSGDSDFTVMKKITSGQIDFAPLETVDLPEGCSDVIRQALKKDRSERYKSAN
                     EMYVDLYHLSRKYFPGEPTSDLSRLVHSMYVKEPVEAEEKATRQNSQQKIKTQILSTP
                     PEPEPVGPKPGPPEERKTGEQKPKREPAAEPEEAKTVIYDSTSEEARTVIHDTSGDEA
                     RTSIYS"
BASE COUNT          467 a          373 c          435 g          373 t
ORIGIN      
        1 atgttgaggg gtgcgggttt gcccgaagag ttcgcttatc tggcattcgt ggaatccggc
       61 tacgacagca cagccacatc ccccgccggc gcccgcgggc tctggcagtt tatgccggca
      121 accgcacgag atttcggcct gaccgtgaat aatgatatgg atgaacgagt taatgcgaaa
      181 aaatccaccg aagctgcttg taaatatatt aaacaattat ataatttgta taactcttat
      241 atgctggcta tggcatccta caacacaggc ctggggagaa tcaggaatgc cctgatgagg
      301 atggatgtga tcgagcaaaa tagattctgg tatcttgtga aaaatgacat gttgcacaat
      361 gagacggtgg agtatgttcc caagataatg gcggcgatga ttattgccac cgatccggtt
      421 aaatttggat tttcggaggc gccggagaaa aaatgagcgc gcgcaggatt atagatgagc
      481 agtatgaaat catccgcgag atcaaagcgg gaggtttcgg cactatatat tacggttggg
      541 atttgactct cgatcgtccc gttgcgataa aggaagttgt taaatctctg ctgggtgaga
      601 agcagtatgt cgatatgttc atcgacgagg ccgtcaatac agcccgcctt aaccatccca
      661 atatagtaca ggtttacagt ctcaggaaaa ctcccgatga tcgcgtcttt ataatcatgc
      721 agtttatcga aggcgtcgat ctgcgcgagg tgatcgatta ctataacgat cgcaacgaga
      781 ccatgccgaa aaacatggcc atgcacatta ttagcgaaat ctgcaaagcg ctcgaatatg
      841 cgcataacct caaggataga aaatccgggc agccattgaa tatcgtgcac agggatatct
      901 cgccctcgaa tatcatgctc accgtggagg gctcggtcaa gctgattgat ttcggcattg
      961 ccaaggcccg caaccgtata gtgcagaaga cccaaaccgg tttcgtcaaa ggtaaggtgg
     1021 cctacatgtc tcccgagcag ctggagggta aagaagctac acgccagagc gatattttct
     1081 cgcttggttc ggttttttac gagatgtgca ccggtgaaga actcttttcg ggcgattcgg
     1141 atttcacggt tatgaaaaag attacctcgg ggcaaattga ttttgcaccc ctggaaactg
     1201 tcgatctgcc cgagggttgt tctgatgtga tccgtcaggc cctgaagaaa gacaggtctg
     1261 agaggtataa gtcagccaat gaaatgtatg tcgacctgta ccatctctcg cgaaaatatt
     1321 ttccgggtga gcccacctcg gatctgtcac gattggtgca ttccatgtat gttaaggagc
     1381 ctgtggaagc tgaggaaaaa gcaacccggc agaacagcca gcagaaaatc aaaacacaaa
     1441 tcctgagtac tccgccggaa cctgaacctg tggggccgaa acccgggccg cctgaagaac
     1501 ggaagacagg ggagcagaag ccgaaaagag aaccggcagc tgaaccggaa gaagccaaaa
     1561 cggtaatcta tgatagtacg tcggaagagg cccggactgt tatccatgac acctcaggag
     1621 acgaagccag aacatcaata tattcaag
//
LOCUS       DSEB01000421            5533 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001050, whole genome shotgun sequence.
ACCESSION   DSEB01000421 DSEB01000000
VERSION     DSEB01000421.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001050"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(112..378)
                     /locus_tag="ENO07_07620"
     CDS             complement(112..378)
                     /locus_tag="ENO07_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01875.1"
                     /translation="MDNFDAPAQHVGESKEKDKEKAEFTLRKEIEKVLGGTLEGAFEL
                     KGGMGLLITIKKGDKPFALVVQRPGNGIDPAKDTDVYISEGIGS"
     gene            complement(403..1392)
                     /gene="amrS"
                     /locus_tag="ENO07_07625"
     CDS             complement(403..1392)
                     /gene="amrS"
                     /locus_tag="ENO07_07625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HDS01876.1"
                     /translation="MMKEAKYYKKLDDKYIKCLLCPVGCKLEEGQEGICLGRKVIDGK
                     LIATNYAQVVAAHVDPIEKKPLYHFHPAELILSVGPNGCNMFCQHCQNWSISQQKQRT
                     MTMMPEDLVSLAEKENSMGIAYTYTEPFIWFDYLMDVCPLAHEKGLYNVLVSNGYIHL
                     KPLEELLPHFDAINVDVKSMRNEFYKDVCQGRFRVVKNTVERIVKAGKHIEITYLIIT
                     DINDSEAEIRELVDWLAGLGDTIPLHFSRYFPSYKMNNPTTPVKTLNMAYAIAREKMK
                     YVYVGNTYIEGTSDTFCPECNNILVNRSGYFTRVTGIENGKCAKCKRPVDFVL"
     gene            complement(1474..2064)
                     /locus_tag="ENO07_07630"
     CDS             complement(1474..2064)
                     /locus_tag="ENO07_07630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HDS01877.1"
                     /translation="MKKPVVFLDRDGTLIEEVNFLLNEKQIKVWPKAIKAMRLLRSKG
                     FLLIVLSNQSGVARGYLTEERVEDLNRTVFNQMRMMGEVPDAFFFCPYHPEAKLERYR
                     RESELRKPDVGMARLAAQRFDIDLSRCYSVGDKLSDVGLGQNLGGKGVLVLTGHGNAE
                     KNKLTFETEYFPDCIVSDILEAAKWIVGDLESIETP"
     gene            complement(2066..3079)
                     /locus_tag="ENO07_07635"
     CDS             complement(2066..3079)
                     /locus_tag="ENO07_07635"
                     /inference="COORDINATES: protein motif:HMM:PF01075.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDS01878.1"
                     /translation="MHSIGIIRLSSLGDIILTEPVTRALKNNFPQTRITYFAKESYRP
                     VLEMFAAVEDIITFEIPGEDEKLGAVKNKTRELPYDFDLVLDLQKNIRSRVILRNLKS
                     SETMTYKKNRLARQIAVWFRRKEGGKHTSEKYLALLDELMVICTDRIPRLAVPEESKL
                     AAKKYLNSIGFESEKFVALAAGASHPPKHYPINQFAELAEIVSSQYGLKILVVEQEHF
                     GYLNLFDKLKSQGVLEFGIGIELKKLAGILENARLVISNDSGIMHLSAALGTPTAGLF
                     GPTHPVLGFAPLGRDTIAITTNEDCCPCSLHGERPCFRDRQYCFLNMTPELVISKVKK
                     YLD"
     gene            complement(3072..3878)
                     /locus_tag="ENO07_07640"
     CDS             complement(3072..3878)
                     /locus_tag="ENO07_07640"
                     /inference="COORDINATES: protein motif:HMM:PF02673.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDS01879.1"
                     /translation="MDILQSIILGLIQGLTEFLPISSSGHLVLGGALFGLESEDITFE
                     VFLHFATFLAVFVFFFRQILKIIISPLRYVFRGSRSDEDLFWSKYLLLIVIGTIPAVI
                     VGLLLKDQIEKAFSSVFLTGVMLLITTAVLFLTLLARPRRTELKLPDGILVGIAQAIA
                     ILPGISRSGSTISTAMFLGVDKKTAFNFSFLLSLPAIFGAFLLQLKEALDMSIEWGVI
                     SIYIPGMIVAFVSGYICLIILRRVVIAGKFAYFGIYTLIIAILAIIFSHA"
     gene            complement(3916..4554)
                     /locus_tag="ENO07_07645"
     CDS             complement(3916..4554)
                     /locus_tag="ENO07_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01880.1"
                     /translation="MKKLTVAALAVLLVVGLSTTGSAQFFMSQLSDARVIGSDEVQVG
                     GGFSVFENLLGLGGFIRIGVINELEVGAKLGFVNFDNDFGDDHTGLTLGADLLYQFLD
                     TEYGDPIDLSTGGGIEYYNFPGDATFLMFGSNTLVSYPVEFESGQVLSPFFRLNLRFD
                     RIDNGVDDTEFELGFGFGAKFDITRYFGFFGEIIIAGGAIDEGFVGGVTFGI"
     gene            complement(4816..5016)
                     /locus_tag="ENO07_07650"
     CDS             complement(4816..5016)
                     /locus_tag="ENO07_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01881.1"
                     /translation="MLSIASIQSFSIFSCLRMAWARSWSSQKSSAVDFFSNSAIFFRR
                     ESRSKITSQLLDFFSIFFKIEI"
     gene            5057..>5533
                     /locus_tag="ENO07_07655"
     CDS             5057..>5533
                     /locus_tag="ENO07_07655"
                     /inference="COORDINATES: protein motif:HMM:PF03462.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HDS01882.1"
                     /translation="MDESGDSAEAVQEFTKAAKEFEEKLSDLELRTFLSEPDDMRDAI
                     VSIHPGAGGTESTDWASMLHRMYMRYADKKGFETELIDYQPGDEAGIKSSTFEIKGDY
                     AYGYLKSESGVHRLVRISPFDANKRRHTSFASVFVYPLIESNIDVDIKDDDIRIDTF"
BASE COUNT         1536 a         1374 c         1208 g         1415 t
ORIGIN      
        1 aataatacag acatgtaatt attcaagtaa aaatatcaaa tataatatgt ttcaggagca
       61 taaaaaatgg gcgcccgaat cggacgcccg caaaattctt ttgcttgaaa atcaggaacc
      121 gatgccttca ctgatataaa catcggtatc ctttgcagga tctataccat tgccgggccg
      181 ttgaacaacc agggcaaaag gcttgtcacc ttttttgatc gtgattaata gtcccatgcc
      241 tccttttagc tcaaaagccc cttccagagt accgccgagc actttctcaa tctctttgcg
      301 cagcgtgaat tccgcctttt ccttgtcttt ttccttggac tcaccgacat gttgcgccgg
      361 ggcatcaaag ttatccatag ctcctccata aggttagtag atttacagga caaaatcgac
      421 aggccttttg catttagcgc atttcccgtt ctctatgccg gttacacgcg tgaaatagcc
      481 cgatcgattc accagtatgt tattgcattc gggacaaaag gtgtccgagg ttccctctat
      541 gtatgtattc cctacatata cgtatttcat tttctccctg gcgatggcat aggccatatt
      601 aagcgtcttg actggagtgg tgggattgtt catcttatag ctcgggaaat aacgcgagaa
      661 atggagcggt atggtatcac ccagacccgc caaccaatcc accagttccc ttatttcagc
      721 ttcggagtca ttgatatctg taataatgag atatgttatc tcgatatgct taccggcttt
      781 aactatcctt tcaactgtat tcttcacaac ccgaaagcgt ccctggcaca catccttgta
      841 gaactcgtta cgcatcgact tgacatcaac attgatggcg tcgaagtgag gcagcaactc
      901 ctccagcggt ttgaggtgta tataaccgtt cgaaaccagc acattgtaaa ggcccttctc
      961 gtgtgcaagc gggcagacat ccatcagata atcaaaccag ataaagggct cggtataggt
     1021 ataggcaata cccatggaat tttctttttc ggcaagggaa accagatctt caggcatcat
     1081 ggtcatggtg cgctgttttt gctgggagat agaccagttc tggcagtgct ggcagaacat
     1141 attgcaaccg tttggcccga ctgagagtat caactctgcg ggatgaaaat gataaagcgg
     1201 cttcttctca atcggatcga catgcgcggc gacgacctgg gcataattgg tcgcaatcaa
     1261 tttgccgtca attactttgc ggcccaggca tatgccctcc tggccttcct caagcttgca
     1321 tcccaccggg caaagcaggc attttatgta tttatcatct aatttcttat agtattttgc
     1381 ttctttcatc atgttttttc aatttgtaaa tgttataaag aattagcaag tattaaattc
     1441 tttaatcacc gcaaagcccc ggattacgcg ggttcacggg gtctctatgg attcaagatc
     1501 gcccacaatc cattttgcgg cctcaagtat atcggaaact atacaatcgg gaaaatattc
     1561 ggtttcaaat gtaagtttat ttttctcagc attgccatgc ccggtgagaa ccagtacacc
     1621 cttgcccccc agattttgac ccaggccaac atcggaaagc ttgtctccga ccgaatagca
     1681 tctgctcaga tcaatatcga acctttgagc agccagtcga gccatgccga catctggttt
     1741 gcggagctcc gattccctgc ggtaccgctc cagtttggcc tcgggatggt aagggcagaa
     1801 aaagaatgca tccggcacct cacccatcat acgcatctga ttgaacacag tccggttcaa
     1861 atcttccacc ctctcctcgg tcagatatcc gcgggccaca ccggattgat ttgacaggac
     1921 tatcagcaga aagcctttgc ttctcaacag ccgcatcgcc ttgatcgcct tcggccagac
     1981 tttaatctgc ttttcattca acagaaagtt gacttcttcg attaaagtgc cgtcacgatc
     2041 aagaaataca accggctttt tcatatcagt ccagatactt tttgactttc gaaatcacca
     2101 attccggagt catattcaag aaacagtatt gtctgtcacg aaagcagggg cgctctccat
     2161 gcagtgaaca gggacagcaa tcttcatttg tggttattgc aatcgtatcc cgccccagcg
     2221 gcgcaaaacc cagaaccgga tgagtcgggc caaaaaggcc ggcggtgggc gtgccgaggg
     2281 cagcggagag atgcattata cctgagtcat tggaaattac aagtctggca ttttccagaa
     2341 taccggcaag tttcttcaat tctattccta ttccaaattc cagtacgcct tgggacttca
     2401 atttatcaaa aaggttcaga tagccgaaat gttcctgttc gaccacaagt atttttagtc
     2461 catactgcga gcttactatc tcagccagtt cagcaaactg gtttatcgga taatgtttgg
     2521 gcggatggga agcgcctgca gctagggcaa caaatttttc actttcaaaa ccgatagaat
     2581 tgaggtattt cttggctgcc agtttcgatt cttcgggcac tgccaaccgg ggtatgcggt
     2641 ctgtgcagat caccatcaac tcatccagaa gggcaagata tttttcggaa gtatgtttgc
     2701 cgccctcttt cctgcgaaac catactgcaa tttgccgggc caggcgattc tttttatagg
     2761 tcattgtctc agaggatttg aggtttctga gtattacacg gctgcggatg tttttttgca
     2821 ggtccagaac caggtcaaaa tcgtagggca gctccctggt cttattcttg acagcgccta
     2881 gtttctcatc ctctcccggt atttcgaatg ttataatatc ctccaccgcc gcaaacatct
     2941 ccagcaccgg gcgataagat tccttggcaa aataggttat cctggtttgc ggaaaattat
     3001 tcttcagggc tcttgtgacc ggctcggtca aaatgatatc gcccagcgag gaaagcctga
     3061 tgataccgat actatgcatg cgagaaaatt atggcaagga ttgcgattat caaagtataa
     3121 attccgaaat atgcaaactt cccagcaatc acaacccttc gtagaattat caggcatata
     3181 tagcctgaaa caaaagccac gatcataccc gggatataga tggaaatcac gccccactcg
     3241 atcgacatat ccagtgcctc tttaagctgc agcagaaatg cgccgaatat tgccgggagt
     3301 gacaacaaaa acgagaaatt gaaagccgtt ttcttatcga cacccagaaa catggcggtt
     3361 gaaatggtcg agcccgagcg cgagattccc ggcagaatcg ctatcgcctg ggcaatgcca
     3421 accagaatac cgtcgggaag cttcaactca gttcgccgcg gcctggccag cagggtcaga
     3481 aataatacgg ctgtggtaat caggagcatt acgccggtca aaaataccga ggaaaaggct
     3541 ttttcgatct gatctttcaa aagcagcccc acaatgactg cgggaattgt cccgataaca
     3601 atcagcaaca aatatttcga ccagaacaga tcctcatcgc tgcgggatcc ccgaaaaacg
     3661 tagcgtaagg gagatataat tattttcaga atctgcctga aaaagaaaac aaatacggcc
     3721 aggaatgtgg caaaatgcag gaagacctcg aaggttatgt cctcggattc gaggccaaat
     3781 aatgccccac ccagcaccag atggccggag gatgatatcg gcagaaattc ggtcaatccc
     3841 tgaatcagcc cgagtatgat agattgcagt atatccataa aaaaagcccc cttccggggg
     3901 cattaataaa tttctctata tcccgaaggt aacgcctccg acaaaacctt cgtcgatggc
     3961 tccgccggca attattatct cgccgaagaa cccgaagtat cgggttatat cgaacttggc
     4021 tccgaaccca aagcccagct caaattcagt gtcatcaact ccgttatcga tcctgtcgaa
     4081 cctgagattc agcctgaaaa atggagaaag cacctggccg ctttcaaatt ccaccgggta
     4141 ggacacgagc gtattgctcc caaacatcaa gaaagtagca tccccgggaa aattataata
     4201 ctcaatacct ccgccggttg agagatcgat aggatcgccg tattcagtat ccaggaattg
     4261 atacaggaga tcagcgccca gggtcaaacc cgtgtggtcg tctccgaaat cattatcaaa
     4321 attaacaaaa cccagcttgg caccaacttc aagctcattg atcaccccaa tcctgataaa
     4381 tcctcccaga cccagcaggt tctcaaagac cgaaaaacct ccgccgactt ggacctcatc
     4441 ggatccgatt acacgtgcat cggaaagctg gctcataaaa aattgcgctg atcccgttgt
     4501 gcttagcccc accaccagta aaacggccaa ggccgcaacc gttagttttt tcatacagta
     4561 ctctcctttt aatcttctcc taaagatttg ccgctaaaga taaaactcta aatccacatg
     4621 tcaatatttt aacatcattt tacctccgct tttatatctt gtttcataaa acaatcaaat
     4681 gctgcaaccc tgtatcatca aatagcatat aaatagttgc aatggaaccg gataatcttt
     4741 caataaactg attgacaaaa catcaacgag gactatattg ttcgctttct aaggagcatt
     4801 attatggctg ctgtatcaga tttcgatctt gaagaatatg gagaaaaaat cgagaagctg
     4861 agaggttatc tttgacctcg attctcgcct gaaaaagatc gccgaattag agaagaaatc
     4921 caccgccgat gacttctggg acgaccagga gcgcgcccag gccatcctca aacagctgaa
     4981 aatcgaaaaa gactggattg atgcgatcga gagcatagaa tcagattatg aagacttaac
     5041 cgtcctgaag gagctggtgg atgagtccgg ggattccgcc gaggcggtgc aggaatttac
     5101 caaagccgcg aaagagttcg aggagaaact ctcggacctc gaattgcgga ccttcttgtc
     5161 tgaaccggat gacatgcgcg acgctatcgt ctcgattcat cccggcgcgg gcggcaccga
     5221 atcgaccgac tgggcctcga tgctgcatcg gatgtatatg cgttatgccg acaaaaaagg
     5281 cttcgagaca gagctgatcg attatcagcc cggggatgag gccgggatca agtcctcgac
     5341 ttttgaaata aaaggcgatt atgcgtacgg ttacctgaaa tcggaatcag gagttcatcg
     5401 gctcgtcagg atatctccat ttgatgccaa caagcgccgg cacacatcat tcgcctcagt
     5461 ctttgtctat cccctgattg agagtaatat agatgtggat attaaggacg atgatattcg
     5521 aatagataca ttc
//
LOCUS       DSEB01000422            1723 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1014058, whole genome shotgun sequence.
ACCESSION   DSEB01000422 DSEB01000000
VERSION     DSEB01000422.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1014058"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1525)
                     /locus_tag="ENO07_07660"
     CDS             complement(<1..1525)
                     /locus_tag="ENO07_07660"
                     /inference="COORDINATES: protein motif:HMM:PF05226.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HDS01883.1"
                     /translation="MKLPGFLKNVQITDVDKSFVRKHPGLILYLLITLLVIALFLEQN
                     RLFENFENKIQDAMFSFRGEQEVGDDVVLLEIDDKAIDYLGWWPWSHGTLARMIEALD
                     YYQPRTVYLDFAIKNNVDDYVSGNSHLLAENIQQSRNVIIPFEAVLGSRTPSTQAAPE
                     WLTASSITPGVISQDDVEKIQRADLPENIFGLASKYLGADVNLFEKDNSVRYQPLILK
                     YENKYYPSVELILSAYYLGIDPNRISYNPGENIQMGNRSIPVTGKGHLLINYLGKSNP
                     FKSYSVKSLWEGQVPVEKLANKLVIVTLTATGQYDHLKTPMTPNMPPGIKTATVVENI
                     IRNDFINSLDASSNAGIVIILGIGLFCAFLLPRVALVHRFTILVVFLFVLVNINFILF
                     SSFNTITKTLYPALEILLFMAAAPMMKSKADIRRKEEKQRRANLQLRKNMTREDKREA
                     EPRERAFTIETDPNVKVRKERRKKADEFAPTTAINTKEHEQVGEETSELSSFDHSVVN
                     "
     gene            complement(1527..>1723)
                     /locus_tag="ENO07_07665"
     CDS             complement(1527..>1723)
                     /locus_tag="ENO07_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDS01884.1"
                     /translation="RYIINVGSVGQPRDGNPDACYVTYDEESKEIYFKRIKYDIKTAQ
                     EKMKQAQIPGYLVERLSLGR"
BASE COUNT          419 a          424 c          400 g          480 t
ORIGIN      
        1 cattcacaac cgagtggtcg aaagatgata attctgatgt ttcctcgccc acctgctcat
       61 gctccttggt atttatagcg gttgtgggag caaattcatc cgcctttttg cggcgttcct
      121 ttctgacttt cacattcggg tcggtttcaa tcgtaaaagc gcgctctctc ggctccgctt
      181 cccgtttatc ttcgcgggtc atgtttttcc gcagctgcaa attcgccctg cgttgctttt
      241 cctctttcct gcggatatcc gctttactct tcatcatcgg agcggcagcc atgaacagaa
      301 gaatctccaa tgccgggtaa agggttttag taattgtgtt gaatgacgag aacagaatga
      361 aattgatatt taccagcaca aacaggaaga caaccaggat ggtgaagcgg tgaaccagcg
      421 ccacacgcgg cagcaaaaat gcgcagaaca gcccgattcc gaggattatg actattcccg
      481 cgttggagga agcatcaagc gagtttataa aatcatttct tattatattt tctacgactg
      541 tagctgtttt tatgcccggt ggcatattgg gggtcatcgg cgttttgagg tgatcgtatt
      601 gcccggtcgc ggtcagggtg actattacca gcttatttgc cagtttctca acgggcacct
      661 gcccctccca cagcgacttg accgaatagg acttgaaagg attcgatttg cccagataat
      721 taatgagcag atgtcctttg ccggtgaccg gaatcgatct gttgcccatc tgaatgtttt
      781 cgcccggatt ataactgatt cggttcgggt cgattcccag gtaataggca gaaagtatca
      841 gttccaccga ggggtaatat ttattctcat atttaagtat caggggctga tagcgtacac
      901 tgttgtcttt ttcgaaaaga tttacatcgg cgcccagata tttggaggcc aggccaaaga
      961 tattctcggg cagatcggct ctctgaatct tctcgacatc atcctgggaa atcacgcccg
     1021 gcgtgattga tgaggctgtc agccattcgg gcgcagcctg agtcgagggc gtacggcttc
     1081 ccaggacagc ctcaaaaggt attataacat ttctggactg ctgaatattc tcagccagca
     1141 gatgtgaatt tccggaaaca taatcatcaa cgttattctt gattgcgaaa tcgagataaa
     1201 ccgtccgggg ctggtaataa tcaagggcct cgatcatacg ggccagggtg ccatgcgacc
     1261 atggccacca tcccaggtaa tcgatagcct tatcgtctat ttccaataaa accacatcat
     1321 ctccaacctc ctgctcgccc cggaaggaga acatggcatc ctgaatctta ttctcgaagt
     1381 tctcgaagag cctattctgt tccagaaaga gcgctattac caggagggta atcagaagat
     1441 agagtatcag gcctggatgt tttctaacga aggacttatc cacatccgtg atctgaacat
     1501 ttttcaaaaa gccgggtaat ttcatgttat cgtcctaaac tcagccgttc caccagatag
     1561 cccggaatct gggcctgctt cattttctcc tgggcggtct taatatcgta tttgatccgc
     1621 ttaaagtaga tttccttact ttcctcgtca taggtgacat agcacgcatc gggattacca
     1681 tctctcggct gtccaaccga gcctacattg ataatatatc ggc
//
LOCUS       DSEB01000423            5526 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001053, whole genome shotgun sequence.
ACCESSION   DSEB01000423 DSEB01000000
VERSION     DSEB01000423.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001053"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..556)
                     /locus_tag="ENO07_07670"
     CDS             complement(<1..556)
                     /locus_tag="ENO07_07670"
                     /inference="COORDINATES: protein motif:HMM:PF00892.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDS01885.1"
                     /translation="MFADMSAVRLYIIIVFGQIVFAATPLFLKIALRDFDPLTLGITR
                     FVISVIILNIFLFIQGRKLIPDKKHWLWIAVLGFLAIPCNQGLLFYGLQFTTPGHAAL
                     MYGLTPLLVYLIVIPLLKEKFRLVKMFGILLAFSGVVLVLSDRQITIEPEFVKGDLII
                     FLGVIGWALYTVFGKGLVKKLGSIL"
     gene            complement(587..1378)
                     /locus_tag="ENO07_07675"
     CDS             complement(587..1378)
                     /locus_tag="ENO07_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDS01886.1"
                     /translation="MDLGLTDKVAIVAASSKGMGKAVAQKLAEEGARVVICSRNNEAL
                     NKAAKDIQEQSGREVLPVKCDVTSSKDLEKLFRTAEKYLKKVDILVCNAGGPPQGTFE
                     KLTERDFSWAIELNLKSTINMCRLAVPGMKKRKWGRIIAITSISAKQPLDDLILSNTV
                     RAGVLGFTKSLSNEMARHNITVNSICPGFTATDRLKDFAAGLAKKSKFTVKQLFTSWE
                     RSIPMNRLAEPEETANLIAFLASEQAAYITGTAIQIDGGYIKSII"
     gene            complement(1380..2747)
                     /locus_tag="ENO07_07680"
     CDS             complement(1380..2747)
                     /locus_tag="ENO07_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HDS01887.1"
                     /translation="MKALEYADKNFDNFVQVLIELLKIPSVSAQSEHKDDMKKCAEFL
                     VDKLRWMGLEPKIFETPGHPVVYAEYINNPQAPTALIYGHYDVQPVDPLELWESPPFE
                     PKIVEDKMYARGSADDKGQIMIHAAAIESFLKTEGSVPINFKIIFEGEEEIQSAHLDT
                     FLEEHKDMLKCDLAIISDTAMFGRDLPALTVSLRGIAIAEIKVVGPNRDLHSGSYGGA
                     VPNPIHELCNIIAKLHDDDHKVTVPGFYDDVVDVADWEREESRKLPFDEKAFLLEVGS
                     KGLVGEKGYTTLERKSARPTLEINGIYGGYSGEGTKTIIPSWAGAKITMRLVPKQDPV
                     KICGLLQKYLTELSPAWVDVQVTTSGGAKPGMVSRENPMMQAAADAIQEGFGKEPLYT
                     REGGSIPIVNVFKEILGVDTILFGFAQPDSNEHSPNEWFDLRDFKRGINSTIHLYKKL
                     AEMKV"
     gene            2936..4528
                     /locus_tag="ENO07_07685"
     CDS             2936..4528
                     /locus_tag="ENO07_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01888.1"
                     /translation="MKKLLIPSTIILVLCWAAVGSADMQTELVIKSFDEYLSYMKEGQ
                     YDQASAQWLPDYIYEVYKFGIFYTNAPYKYDCNSYLTEHLDEYKQDQLEITYSAMPSM
                     YEVYKILARYDENSGNGNSGAQDEYYLMKGADANFYLVPNYWPLLPNMDSKEGKYYKL
                     YYNKESQINDSALAHIDLLVDSIARRLELSPQLLERLSMDKFNYFLCENANQIAIYAG
                     RYVPGWHDPAGDFIITNYLPHGTPMADFLIKYKLSELPLHTLPFMEKGLPVYLGGRAG
                     ADLGVFGQMVRFSLESDFMKLEDILSAADFEEKTGGPDFGYTLSAYFVGYLVEELGIG
                     GVLDIYLQLSGDGEFVDTCSMNTVKSILENQTGRDWQSLESGFEDYFNQVEFNMDLVE
                     KASGNVLYQSGTPGISVSLYMDGDYLILDAYANDEGSAVNAAMLLNNTGADITGNYRS
                     SLFEKHFPEREYDGQYYGLIFGPEELGVYNYLTNEIAAKYISSLADPDQQVDPSHITF
                     KIPRSILPGDFGQLTLEVFEMP"
     gene            4623..5516
                     /locus_tag="ENO07_07690"
     CDS             4623..5516
                     /locus_tag="ENO07_07690"
                     /inference="COORDINATES: protein motif:HMM:PF05368.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HDS01889.1"
                     /translation="MRLFVTGASGYFGSNLLPRLIEAGHKLVCLIRKGSEKKIEKYLE
                     DIEIISGGISEIPSWKDQLEDIDAFINLIGIIREFPSRGITFEKLHFRTTKSLADLAS
                     ERGIKRFLQMSALGASPDSKAEYHRTKYKAEQYLQESGLAWTIFRPAFIIGKGNDFVE
                     TVLNLMDSAPLFPIIGSGEYRMQPVDVDNVTEGFVKSLSEQTASGKIYEIGGPDRFSY
                     NRMVDLIAEARGKKARKIHLPVFMMKGVAGLFEYFKSFPLTKGQINMLLEENFTDSRE
                     FFEDLNITPIIFDESIKKALS"
BASE COUNT         1411 a         1335 c         1339 g         1441 t
ORIGIN      
        1 ccagaataga gcccagtttt ttgaccagtc cttttccgaa aactgtgtaa agcgcccacc
       61 cgatcacgcc caggaaaatt atcaggtcgc cttttacaaa ttcaggctct atcgtaatct
      121 ggcgatccga aagcaccagt accactccgg agaacgccag cagtattcca aacattttaa
      181 caagtctgaa tttctccttc aagagtggaa ttacaattag atagaccagt aaaggagtga
      241 gaccgtacat caaggcggca tgcccgggag tcgtgaactg caatccataa aagagcaagc
      301 cctgattgca gggaatggcc aggaagccca gtacagcaat ccagagccaa tgctttttat
      361 cggggattag cttccttccc tgtatgaaca ggaaaatatt gaggatgatg actgagatta
      421 caaatctcgt aattcccaat gtgagtggat caaaatcacg taaggcgatc tttagaaaga
      481 ggggagtggc ggcaaataca atttgcccga aaactattat aatatataat ctcacggcag
      541 acatatctgc aaacaccgcc gactgctaaa aaacgttttt ctctggctaa ataatgctct
      601 tgatatatcc gccgtcaatc tggatcgcgg ttccggtaat ataagccgcc tgctcggatg
      661 ccagaaaggc gatcaggttg gcggtctcct ccggttcggc cagacgattc atggggatcg
      721 agcgctccca tgatgtgaac aactgcttca ctgtaaattt ggattttttt gcgagtcccg
      781 ccgcaaaatc cttgagccgg tcggtagcgg tgaaaccggg gcagatcgaa ttgaccgtta
      841 tattatgccg ggccatctcg tttgaaagag acttggtaaa gcccaggact cccgcccgca
      901 cggtgttgga taaaatcagg tcatccagag gctgcttcgc ggaaatagat gttatggcga
      961 tgatccgtcc ccatttacgc ttcttcatgc ccggcactgc cagcctgcac atgtttatcg
     1021 tggatttcag attaagttcg atggcccatg aaaaatcccg ctcagtaagc ttctcaaatg
     1081 tcccctgcgg cgggccgccg gcattgcata cgagaatgtc cacctttttc aagtatttct
     1141 ccgccgtcct gaataatttt tcgaggtctt tggatgaggt gacatcacat ttgaccggca
     1201 gaacctcccg tccactctgc tcctgaatgt ctttggccgc cttgttcaaa gcctcattat
     1261 tgcggctgca gattacaacc ctggcgccct cctcggccag cttttgggcc acagccttac
     1321 ccattccttt tgacgaggcc gcgactatcg ctactttgtc tgttaagccc aaatccatgt
     1381 cacaccttca tttcagctaa ttttttataa agatgaatag tggaattaat tccccgcttg
     1441 aaatccctca agtcaaacca ttcattgggc gaatgctcat tcgaatccgg ctgtgcaaaa
     1501 ccgaacagaa tggtgtccac tcccagaatc tccttgaaaa cgtttacgat cggtatcgaa
     1561 ccgccttctc tggtataaag aggctccttg ccgaaacctt cctgtatagc gtcagccgcc
     1621 gcctgcatca tcggattttc ccttgacacc ataccgggct tggctccgcc cgaagtggtc
     1681 acctgaacat cgacccaggc gggagacagc tctgtcaggt atttctgcag cagaccgcag
     1741 attttgaccg ggtcctgctt gggaaccagg cgcatggtga ttttagctcc ggcccaggac
     1801 ggtataattg tcttagtgcc ctcaccgctg taacccccgt agatgccgtt gatctcgagc
     1861 gttggccggg ccgacttacg ttccaaagtg gtatagccct tttccccgac cagacctttg
     1921 ctgccgactt ccagaaggaa cgccttctca tcaaagggga gctttctgct ttcctcgcgc
     1981 tcccagtcgg cgacatcaac aacatcatca tagaacccgg ggacggtcac tttatggtca
     2041 tcatcatgaa gcttcgcaat aatattgcag agctcatgaa tcgggttggg cacagctccg
     2101 ccgtacgatc cggaatggag atcgcgattg gggccgacca cctttatctc cgcgatcgct
     2161 attcccctca ggctcacggt aagcgcgggc aggtcgcggc caaacattgc ggtatcggag
     2221 atgatcgcca ggtcacactt gagcatatcc ttgtgctctt ccaggaatgt atctaaatga
     2281 gccgactgaa tctcctcttc accctcgaat ataattttga aattgatggg tacgctgcct
     2341 tcggttttga ggaacgattc aatcgcggcg gcatgtatca taatctgccc tttatcgtcg
     2401 gccgaaccgc gggcatacat tttatcttcc acgattttcg gttcaaacgg aggggattcc
     2461 cagagctcca ggggatcgac cggctggaca tcgtaatgac catagatcag cgcggtgggc
     2521 gcctgcgggt tattgatata ttcggcatac acgaccggat ggccgggggt ctcgaatatc
     2581 tttggctcaa gtcccatcca cctcaattta tccaccagga attcggcgca ttttttcata
     2641 tcgtccttgt gctcggattg tgctgaaact gaggggattt tgagcagttc gatcagaacc
     2701 tgtacgaaat tatcgaaatt tttgtcagca tattctaatg ctttcatcga agcctcctta
     2761 ttgtaaagga ttagcttaaa ttgccggggc ctcgatgtca aggcaattct tttttattcc
     2821 tgttatttgg aaaaattggt ggaaatttca ggcgataata ttatatatgg cagggccgat
     2881 aatttgaacc atatactcga cctgttgtaa aaatggataa ctatcggagg aacatgtgaa
     2941 gaagctatta ataccatcca caataattct tgttttgtgc tgggctgcgg tcggttcggc
     3001 cgatatgcaa actgagctgg taatcaaaag tttcgatgaa tatctgtctt acatgaaaga
     3061 ggggcagtat gatcaggcct cggctcaatg gcttcccgat tatatttacg aggtgtacaa
     3121 attcggcatc ttctatacga atgctcccta taaatacgac tgcaattctt acctgactga
     3181 acacctggat gaatataaac aggaccagct tgaaataacc tacagcgcca tgcccagcat
     3241 gtatgaggtt tacaagatac tggccaggta cgatgaaaat agcgggaatg ggaattccgg
     3301 ggctcaggac gaatattact tgatgaaggg tgccgatgcg aatttttacc tggtccccaa
     3361 ttactggccc ctgctcccca acatggacag caaagaggga aaatactaca agctttatta
     3421 taataaagaa agtcagataa acgacagcgc gctggcgcat atcgatctgc ttgttgatag
     3481 tatcgcacgg aggctggaac tttcacccca gcttctggag cgcctgagca tggacaagtt
     3541 caattatttt ctgtgcgaga atgccaacca gattgcgata tatgccggca ggtacgtccc
     3601 cggatggcac gatccggccg gagacttcat catcacgaat tatcttcccc atggcacacc
     3661 gatggcggat tttctgataa aatataaact cagcgagctg cccctgcaca ctttgccttt
     3721 catggagaag ggcctgcctg tatatctggg tggaagagct ggtgcggacc tcggcgtttt
     3781 cgggcagatg gtcaggttca gcctggaatc cgattttatg aagctggaag atatcctgag
     3841 cgccgcagat tttgaggaaa agaccggcgg gcccgatttc ggttataccc tttcagccta
     3901 ttttgtcgga tatctggtag aagaacttgg gattggcggg gtcctggaca tatatctgca
     3961 attatccgga gacggggaat ttgtcgatac ttgctcgatg aatacggtca agagcattct
     4021 ggagaatcaa accggccggg actggcagtc gctggaaagc ggattcgaag attattttaa
     4081 tcaggttgaa ttcaatatgg atctcgttga gaaggcaagc ggaaatgtcc tctatcaatc
     4141 cggtaccccc gggatttccg tttcgcttta catggatggg gattacctta ttctcgatgc
     4201 ttatgcaaat gatgaaggtt ctgcggttaa cgcggccatg ctccttaata ataccggagc
     4261 ggatataacc ggaaattaca ggtcttcgct gtttgaaaaa catttccccg agcgtgaata
     4321 tgacgggcag tattacggcc tgatatttgg gccggaggaa ttgggcgtat ataactatct
     4381 caccaatgaa attgccgcta aatatataag ctcgctggcc gatcccgatc agcaggttga
     4441 tccctcacat ataactttta aaatacccag gagtatcctg ccgggagatt tcggtcagtt
     4501 gacgctggaa gttttcgaga tgccctgacg acatcaggct gattctctga ttcctggaac
     4561 tatttatttt tatttacatt tctgtaatca aagtatatag taatttcaaa atcggagtgt
     4621 atatgcgtct ttttgttaca ggagcgagcg gctatttcgg aagcaacctg cttccgcgtt
     4681 tgatagaagc gggacacaag ctggtatgcc ttatccgcaa gggctctgaa aagaagatcg
     4741 agaaatacct ggaggatatc gagattatct ccggcggcat ttccgagata ccttcctgga
     4801 aagaccagct cgaggatata gacgcattta taaacctgat cggcatcatt cgagagtttc
     4861 cctcaagagg gataacattt gagaagcttc atttccggac aaccaaatcg ctggccgatc
     4921 ttgcctccga gagaggtatc aagcgctttc tgcagatgag cgcgctgggg gcctcgcccg
     4981 actccaaagc tgaataccac agaacaaaat ataaagccga gcagtatctg caggagagcg
     5041 gcctggcctg gactattttc cgtccagctt tcataatcgg caaaggcaat gattttgtgg
     5101 aaaccgtttt aaatctcatg gattcggctc cgctttttcc cataatcggt agcggggagt
     5161 atcgtatgca gccggttgat gtggataatg taaccgaggg ctttgtcaaa tcactgagcg
     5221 aacagacagc ctctggaaag atttatgaaa tcggcgggcc cgaccgcttc agctataatc
     5281 gcatggtgga tctgatcgcc gaggcgcgcg gtaagaaagc ccgcaagata catcttccgg
     5341 ttttcatgat gaaaggcgtc gccggcctct tcgaatattt taagtcgttc cctttgacca
     5401 aagggcagat caatatgttg ttggaagaga attttactga ctcgagggag ttcttcgagg
     5461 acttgaatat aacgccgatc atttttgacg agagcatcaa gaaagctctc tcgtaataaa
     5521 aggaaa
//
LOCUS       DSEB01000424            2971 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004202, whole genome shotgun sequence.
ACCESSION   DSEB01000424 DSEB01000000
VERSION     DSEB01000424.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004202"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            95..>2971
                     /locus_tag="ENO07_07695"
     CDS             95..>2971
                     /locus_tag="ENO07_07695"
                     /inference="COORDINATES: protein
                     motif:HMM:PF07676.10,HMM:PF14684.4,HMM:PF14685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HDS01890.1"
                     /translation="MYRRSCLSILGRAIFMIFLGLASLSAQDLQEMPIMLQPDVHDTL
                     VAFVYGGDIWTVNTEGGVARRITIHDGDESYPKFSPDGKLIAFTGSYDGNSDVYVMNL
                     FGGDITRITYHPGSDDIVGWHPLNGKIMFRSLRQNFMTGSRLYMISPDGTGLEDIPIH
                     EIYFGTFSEDGKQIAYTHVGREERNWKRYYGGTAEDVLHYDFDTKELRHLTDFKGSDR
                     FPLWIGEKIYFNSDRSYRLNIFSYDLSTKEIEQVTYHEDFDVRRPSMGGSKIVYELGG
                     SLWLLDVTTSETKRIPVQIETDLPERRPRFVKAAEHITYADCAPDGRAGLVVARGDVF
                     TVTKEAGLTENLTESSGSRQREAVWSPDGEWVAFFSDERGEYEIHLIDPGGEKQPIQL
                     TDHKKGYRHALRWSPDSKKLAYTDETLTLYYIDVASRRITKVDKANYEFMDVSFDKKE
                     ISDYSWSPDSRFIVYSRMNSDLMLQLYIYSLENGQIYHISDGFYHDFSPVFTKDGNHI
                     LFASNRLFDPTFCDYEWEMVFKDVAGIFAITLRKDGQPFLPLEGAEQDDTGKAENGNV
                     LIDFDGIKERIEKLPLEKGNYRKLAVNDSRLFYLNKDKGDFNFFELRDVGPMDLYAYS
                     FEDRKESEVMKNIEDYKISADGSSIVYRQRDKVGIISSSATSSSGDPLELSRVEMRLD
                     PVAEWYQIFDDAWRIERDFFYDPNLHGMDWPAIGDKYRRLIEYAANRQDVEYIIGELI
                     AELSTSHTYVYSGERYRKAERVNVGMLGADFEIDQENNLYRIKKMYSASYWNMDSRSP
                     LGRIGVDVNVGDYLLAVNGDLITADSSIYSYFQNLANQQVTLLVNDRPTTQGAREVIT
                     ETSESEFVLRYLSWVEENRRIVDSVSNGLIGYHFFPDTYTSSARSFPGQFYPQTQKKG
                     IILDGRNNGGGLDPDIFLNRYRKKVLAYWTRRHYFFHPVLYPSSG"
BASE COUNT          782 a          635 c          775 g          779 t
ORIGIN      
        1 actataatta ttttgtctcg tatatgagag aaattacggg gtgctctaag cgctggtgaa
       61 tttggcaaaa tataaaatca tacaggagtt taagatgtat cgcagatcgt gtttatccat
      121 tttgggtaga gctattttca tgatattcct gggtctggcc tctctttcag cgcaggattt
      181 gcaggagatg cctattatgc ttcagcctga tgttcatgat acacttgtgg cttttgttta
      241 tggcggggat atctggacgg tgaataccga ggggggagtg gcacgccgaa taaccatcca
      301 cgatggcgac gaaagctatc cgaaattctc tccggatggg aagttgatcg cttttaccgg
      361 ttcgtatgac gggaacagtg atgtctatgt tatgaatctc ttcggcggcg atataacacg
      421 tatcacctat catcccggtt ctgacgacat cgttggctgg catccgctaa atggcaagat
      481 aatgttccgc tcattgcgtc agaacttcat gaccgggtcg cgcctgtata tgatatcccc
      541 ggatggcacg ggtctcgagg acataccgat tcatgaaata tacttcggta cattttcgga
      601 ggatgggaag cagatagcct acacgcatgt cggcagggaa gaacgcaact ggaaaagata
      661 ttatgggggc acggcggaag atgtcctgca ttatgatttt gatacgaagg agctgagaca
      721 ccttacggat ttcaagggct ccgacagatt tcctctctgg atcggtgaaa aaatatattt
      781 caactccgac cgcagttacc gcttgaatat tttctcttat gatctcagca ctaaagagat
      841 tgagcaggtt acatatcatg aggattttga tgttcgtcgg cccagcatgg gaggaagcaa
      901 aatagtatat gagcttggag gttcactatg gctgctggat gtcactacaa gtgagaccaa
      961 acggataccg gttcaaattg agaccgacct tccggagaga cgcccgcgat ttgtgaaagc
     1021 ggcagaacat attacatatg ccgattgcgc tcccgacggc agggccggac tggtagtggc
     1081 ccggggggat gttttcactg tcaccaaaga agcgggcctg accgaaaatt taacggagtc
     1141 atcgggatcg cgtcagcgcg aggcggtatg gtctcctgac ggtgaatggg ttgccttttt
     1201 ttcggatgaa agaggagagt atgaaattca tctgattgat ccggggggag aaaagcagcc
     1261 gatccagtta accgatcata aaaagggtta caggcatgcc ctgcgctggt ctcccgatag
     1321 taagaaactg gcttacactg atgaaaccct gaccctttac tatatcgatg tcgcgagccg
     1381 caggattaca aaagtggata aggccaatta tgagtttatg gatgtttcat ttgataagaa
     1441 agagatctct gattattcct ggtcacccga cagccgcttt atcgtctact ccagaatgaa
     1501 ttcagacctt atgctgcagc tttatatcta ttctcttgaa aacggccaga tatatcatat
     1561 ctctgatgga ttctaccacg atttctcgcc ggtctttacc aaagatggta atcatattct
     1621 ctttgcctca aacaggcttt tcgatccgac tttttgcgac tatgaatggg aaatggtctt
     1681 caaggatgtg gccggaatat ttgctatcac gcttagaaag gacgggcagc cgtttttgcc
     1741 tctggaaggc gcagagcagg atgacaccgg caaagctgaa aacggaaatg tgctgatcga
     1801 ctttgatggc ataaaagaaa gaattgagaa gctgcctctg gaaaaaggta attaccgtaa
     1861 acttgctgtc aatgattccc ggttgtttta cctgaacaaa gataagggtg atttcaactt
     1921 ttttgagctt cgggatgtgg ggccgatgga tttatatgcc tactcgtttg aagacaggaa
     1981 agaatccgag gtcatgaaaa atattgagga ttacaaaatt tcggccgatg gctcgagcat
     2041 tgtgtaccgc cagagagaca aggtcgggat tataagctca agcgccacca gctcaagcgg
     2101 cgatccgctt gagctctccc gggtggaaat gcggctcgat ccggttgccg aatggtatca
     2161 gatatttgat gatgcctgga gaatcgaaag agacttcttc tatgatccca atttgcatgg
     2221 catggactgg cccgcaatcg gcgataagta tcgcaggctt atagaatatg ccgccaaccg
     2281 ccaggatgtg gagtatataa tcggggaact gatcgcggaa ttaagcacct cccacacgta
     2341 tgtctattca ggtgagcgtt acaggaaagc tgaaagggtc aatgtgggca tgctgggggc
     2401 ggattttgag attgaccagg aaaataatct ttaccggatc aagaagatgt attccgcgtc
     2461 atactggaat atggattcca ggtcgcctct gggaagaatc ggggttgatg ttaacgtggg
     2521 tgattacttg ctcgctgtta acggggatct gattaccgcc gacagcagca tctacagcta
     2581 tttccaaaat ctcgccaatc agcaggtaac actcctggta aatgatcgtc cgaccacgca
     2641 gggggccagg gaggtcatta ccgagacctc tgaatcagaa ttcgttctgc gctacctgag
     2701 ctgggtggaa gaaaaccgca ggattgtcga ttctgtttcg aacggcctga taggttacca
     2761 ttttttcccg gatacatata cctcgagcgc aagatctttc ccggggcagt tttatccgca
     2821 gacccagaag aagggtataa tactggatgg aagaaataat ggcggcggtc tcgatcccga
     2881 tatctttctt aaccgttaca ggaaaaaagt gctggcctac tggacccgtc gccattattt
     2941 cttccatcca gtattatacc cttcttctgg g
//
LOCUS       DSEB01000425            2553 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1005897, whole genome shotgun sequence.
ACCESSION   DSEB01000425 DSEB01000000
VERSION     DSEB01000425.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1005897"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..541
                     /gene="lnt"
                     /locus_tag="ENO07_07700"
     CDS             <1..541
                     /gene="lnt"
                     /locus_tag="ENO07_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HDS01891.1"
                     /translation="YIPLEKLFFFAETITRDVGAFQSGKDYKVINIGGKPANAIICFE
                     AVFPDLVRRFVRQGSRLIINLTNDAWYGDSAAPYQHFAIARFRAVENRRFLIRAANSG
                     ISGIIDPGGRVQGATGLLQEAISRGRFAFLDVKTVYTRYGDAFVFLCAIISCALWIAA
                     GFRRASQPDNSGIGRRAGK"
     gene            777..1694
                     /locus_tag="ENO07_07705"
     CDS             777..1694
                     /locus_tag="ENO07_07705"
                     /inference="COORDINATES: protein motif:HMM:PF07900.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1670 domain-containing protein"
                     /protein_id="HDS01892.1"
                     /translation="MELAVWKEPGGRLEDCQFQGRFTHKPDTSMEFSMINNTSLAHWA
                     RLQAKNLDAQFTNEMVHGLNCSPFEAEAIMEKVHEIYAPLIDAAQGLHPGQIQIIAVD
                     ASVAPNTPLREAKQKLVTLTLDAGIEDIEVRKKGSIPALRQKRLCRMCLEAFQQGAAL
                     TLEDLARLFNCGVRTLVTDLEVLRKEGTIPPLRSMVRDMGRAITHRKLIITLWMQGYE
                     YSDIAKRTCHSVDSVANYVDKFKRCAALFLSGFDLHTVALMVKLSPSLTREFQKLHSE
                     LKGVPHRLQELADLLKKNRVRTLKQGRHA"
     gene            1691..2443
                     /locus_tag="ENO07_07710"
     CDS             1691..2443
                     /locus_tag="ENO07_07710"
                     /inference="COORDINATES: protein motif:HMM:PF07900.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1670 domain-containing protein"
                     /protein_id="HDS01893.1"
                     /translation="MIPQPQSQKHYGSAHRRFLQSSIENFFDTEFPRIFGPVIRQKIA
                     EKIVELVNLQLPNRDHLRPGQCVWNAVSVKTRPNSPNCKFVPVILTLVDPSDIEELTK
                     GTPMSTIAQKATARLCREASDQGALLSMRDIGLLVWRPNCQISSIRKKWETENNVTSL
                     PHVGTVQDFGTCLTHKTSIVRKVIYEKKDPRRVADETKHSQRAVDRYVKDFNRVRICH
                     QHSKDMEFITRTTGLSKYLVRQYVQIINDNQK"
BASE COUNT          687 a          617 c          634 g          615 t
ORIGIN      
        1 atatataccg cttgaaaagc tcttcttttt tgccgaaacc attacaaggg atgtgggcgc
       61 tttccaatcc ggaaaggact ataaggtaat caatatcgga ggcaagcccg cgaacgccat
      121 tatctgtttt gaggcggttt tccctgatct ggtcaggcgt tttgtgcggc aggggagcag
      181 gttgataatc aatttgacga atgatgcatg gtatggcgac agtgcagcgc cttatcagca
      241 tttcgctatt gcgcgattcc gggctgtcga gaatcgcagg tttttgatcc gggcggccaa
      301 ttcgggtatt tcgggcatta tcgatcccgg cggaagagtg cagggcgcga cggggttgct
      361 gcaggaagcc ataagcagag gacggtttgc attccttgat gtgaagacgg tgtacacgcg
      421 ttatggcgat gcattcgttt ttctatgtgc tatcatttcg tgtgcgttgt ggatagctgc
      481 cggattccgt cgcgcgtcgc agcccgataa ttccggaatt ggacgcagag caggcaaata
      541 acaaagtggg cgagagccct gggcctgaag tttataattt gagaccggat tgaaatgagg
      601 gacccagaca gccgcgaagc ggctgcgaaa cgaagcccag ggttggcttc ttgcacggtt
      661 cctcccaggg ttgaccgcaa aaaacgcggc caaccctggg ctctgatgcg aagcccttat
      721 gattgtgcaa tatcgcgtgt tgatataaaa gaacatggaa atcctagtga agagatatgg
      781 aattggcagt ctggaaggag cccggagggc gactggaaga ctgccaattc caaggccgat
      841 ttactcataa gccagatacc tcgatggagt tctcgatgat caacaacaca agtcttgccc
      901 attgggccag acttcaagca aaaaacctag acgctcagtt tactaacgaa atggtgcatg
      961 gccttaattg ttctccattt gaagcagaag ctatcatgga gaaagtccat gagatctatg
     1021 ctccattgat agatgccgca caaggacttc atccaggcca gattcaaata atagccgtag
     1081 atgcctctgt tgcacccaat acgccattgc gtgaagccaa gcaaaagctt gtgactctta
     1141 cattggatgc cggcattgaa gatatcgaag tccgtaagaa aggatcgatc cccgctcttc
     1201 gccaaaagcg gttgtgccgt atgtgcctgg aagcattcca gcaaggagcg gctctgacgc
     1261 tggaggatct ggcacgattg tttaattgcg gcgtgcgcac cctcgtaact gaccttgagg
     1321 ttctgcgaaa agaaggcacc attcctcctt tacgttccat ggtaagggat atggggagag
     1381 ccattacaca tcgcaagctc atcataacgc tttggatgca gggatatgaa tactccgata
     1441 ttgctaaaag gacctgtcat agtgttgatt ctgtggctaa ttatgtcgat aaattcaagc
     1501 gttgcgccgc cctctttctt tccggtttcg atcttcatac cgttgcttta atggtaaagc
     1561 tctctccatc gttaacccgt gaattccaga agctgcattc agagttaaag ggtgtccccc
     1621 atcgattgca ggaattagcg gatttgctta aaaaaaaccg agtccgaaca ctgaaacagg
     1681 ggaggcacgc atgatccctc aaccccaatc tcaaaagcat tacggctcgg ctcaccgacg
     1741 attcctccaa tcttcaatcg aaaacttttt tgacaccgag tttccaagga ttttcggacc
     1801 ggtcattcgg cagaaaatag ccgaaaagat cgtcgagcta gtcaacctgc aattgccgaa
     1861 ccgagatcac ctgcgcccgg gacagtgcgt ctggaatgcc gtctctgtca agactaggcc
     1921 gaatagtcca aactgcaaat tcgttcccgt cattcttacc cttgtcgatc cctctgatat
     1981 tgaagaactt actaaaggaa cacccatgtc caccattgct caaaaggcaa cggcccgctt
     2041 atgtcgagaa gcatctgacc aaggcgcgct gctttccatg cgggatattg ggctgctggt
     2101 atggcgccca aactgccaaa tctcaagcat tcgaaaaaaa tgggaaaccg aaaacaatgt
     2161 gacctcgcta ccccacgtcg gtacggttca agacttcggc acatgcctca ctcacaaaac
     2221 atccatcgta cgcaaagtca tttatgagaa aaaagatcct cgccgcgtag cggatgaaac
     2281 caagcacagt cagagggctg ttgatcgata cgttaaggat ttcaatcgcg ttagaatttg
     2341 ccatcagcat tcgaaagaca tggaatttat aaccaggaca accggtttat cgaaatatct
     2401 tgtacgccaa tatgttcaga tcattaacga caaccaaaaa tagccttgac cacgcaatat
     2461 tgcgtaaatt tcggggcttc tgcagagcag acgtcagatt tgccattaga aagatcatgc
     2521 aaagcgcggc atgaatgcag gatccctctg aat
//
LOCUS       DSEB01000426            3223 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003531, whole genome shotgun sequence.
ACCESSION   DSEB01000426 DSEB01000000
VERSION     DSEB01000426.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003531"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            26..337
                     /gene="csrA"
                     /locus_tag="ENO07_07715"
     CDS             26..337
                     /gene="csrA"
                     /locus_tag="ENO07_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HDS01894.1"
                     /translation="MLILTRKLGESITIGDNIKVTVLGIYGRQVRLGIDAPLKVVVHR
                     EEIYVKIQKENRKASKTEKEDLNNAVDYLKGKYKDDIKKSDSKPKMRYRDDRSKRPPK
                     P"
     gene            306..2351
                     /gene="flgL"
                     /locus_tag="ENO07_07720"
     CDS             306..2351
                     /gene="flgL"
                     /locus_tag="ENO07_07720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein 3"
                     /protein_id="HDS01895.1"
                     /translation="MTVPRGRPNPDVNGALLIADFVFSRHRKYKRLWRIHKVMKITSK
                     MISDLVNKRQSRNVNHFHNLQDRMYIGRCIMKASDDQAGTTKTFSYRSRISEIEQYRA
                     NIVEGSRWLASVDAALENIIEIIRQVKEIAVSMANNTYDAAARDSMADQVRSLFRQML
                     QAGNLQQNSRYLFSGLMTRTEPFAASSRGVVYNGDQGQVQYEIETATRISINIPGSDI
                     FTRAFRILGEDSDLRAGIDGNTQLADLNSGRGVDLSPGSFIITDQNLGNSVTVTIPPG
                     TASIESMMAGINSQLAAAGIDNLTVRLGDQRNNLRLAAADKPQVSLSTPLANLNYGHG
                     IGSEPYEIRIHSADYSTDLTVDLSAASTIGDVINEINATLVDAGIDRVTAGLNAALSG
                     IDIEDTNIASLNLQVSEISNDSLTGAALHILGNISPLLSGQDLNPRPDFSVEEVAPGN
                     TTAADIGLLGNFHYDLVGNDLDPQITPLTPLALINNGSGCQLDKIMISQGSSSLTVDP
                     GTTGIATIGDLMNIINSCGLPIQASINENHKGLQVESTVIGRTLLIEDSGTSDTAYNL
                     GIEGSPDVLGNLIFLVDAIRNNDKKPIGKVIASLDSSINHILNERASMQAKMIRLHTA
                     ESRLMDYSLELTKLLSETEGEDLSELAADLASHEHIFSAALNSAARIIQPSLMDFIR"
     gene            2359..2838
                     /locus_tag="ENO07_07725"
     CDS             2359..2838
                     /locus_tag="ENO07_07725"
                     /inference="COORDINATES: protein motif:HMM:PF02623.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HDS01896.1"
                     /translation="MKIATFRFGELEIPEENVIEFPKGLIGFEQFKQFVLLERRDSEP
                     FRWLQSLEDPDLAFVVVNPVIFFKYYKIEVHYKELEDIQVSTLDRTQIFTVITIPDDI
                     SRMSANLLGPLVINLDNNCGKQIVLSNSTYTTRHYLLDELNKGARELAGKHSLAVQI"
     gene            2932..>3223
                     /locus_tag="ENO07_07730"
     CDS             2932..>3223
                     /locus_tag="ENO07_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01897.1"
                     /translation="MPKKKQKPKAKDISAPGRKKRPAGKAKSVSSSGPGRSEQYTEAL
                     KKAEDFLDRGKHEEAVIAAGRLLTPRIREKDPTLFLSAARISAFANVNLRNYI"
BASE COUNT          909 a          745 c          825 g          744 t
ORIGIN      
        1 atactaagta tggtgggaga caaaattgct tattctaaca aggaagttgg gcgaaagcat
       61 taccatcggt gacaatatca aggtcaccgt gctggggatc tacggacggc aggtgcggct
      121 cgggatcgac gctcctctca aggtggtggt gcacagggaa gagatttatg tcaaaataca
      181 gaaggaaaac cgcaaggcct caaaaaccga aaaggaagat ctcaacaatg cggttgatta
      241 cctgaaagga aaatataagg acgacatcaa gaaatccgat tccaaaccca aaatgcggta
      301 tcgggatgac cgttccaaga ggccgcccaa accctgatgt aaacggggcc ttattgattg
      361 cggatttcgt cttttcccgg catagaaaat ataaaagact ttggcggatt cataaggtta
      421 tgaaaataac cagtaaaatg atatcggatt tggttaacaa aagacaatcc cggaatgtaa
      481 accattttca caacctccag gatcggatgt acattggaag gtgcattatg aaagcctcgg
      541 atgatcaagc aggcacgacc aagacattct cataccgctc gcgtatttcc gagatcgagc
      601 aataccgggc caatattgtc gaaggcagtc gctggctggc ctcggttgat gccgccctgg
      661 aaaatattat cgaaattatc aggcaggtta aagaaattgc ggtgtcgatg gccaataata
      721 cttatgacgc cgctgctcgc gattccatgg ctgatcaagt ccggtccctg tttcggcaga
      781 tgctgcaggc ggggaatctc cagcagaact cgcgctacct gttttccggg ctaatgaccc
      841 gtacagaacc ttttgcggcc tcatccagag gggtagtcta taatggtgac cagggacaag
      901 tacaatatga gattgaaact gcgaccagga tcagcatcaa tataccgggc tcggatattt
      961 tcaccagggc attcagaatt ctgggggaag acagcgatct cagggcaggt atcgacggga
     1021 atactcagct ggcggattta aacagcgggc ggggtgtaga tctctctccc ggatcgttca
     1081 ttattacaga ccagaatctg ggtaactccg taactgtaac aatacctccc ggaaccgcca
     1141 gcatagagag catgatggcc gggattaaca gtcagcttgc ggccgctgga atagataatc
     1201 tcacagtccg gctcggcgac cagcgaaata atctcaggct ggccgccgcg gacaagccgc
     1261 aggtgtcgct atccacacca ctcgcaaacc ttaattacgg gcacgggata ggctcggaac
     1321 cgtatgagat tcgcatccac agcgcggact actcgacaga cttaactgtc gacctcagcg
     1381 cggcatccac gataggagat gtcatcaatg aaataaatgc taccctggtg gatgccggta
     1441 ttgaccgggt tacggccggc ctgaatgcag ccttatccgg aatagacatc gaggatacaa
     1501 atatcgcttc tctgaatctg caggtttcgg aaatctcgaa tgattcgctt accggagcgg
     1561 cactgcacat acttggcaac atttcgcctc tcctgtccgg gcaggatctc aatccgcggc
     1621 ccgatttcag cgttgaggag gttgctcccg ggaatacgac tgctgcagat ataggactcc
     1681 tggggaattt ccattatgat ctggtaggca atgatctcga tccgcagatc acccctttga
     1741 cccctcttgc tctgataaat aatggatcgg gctgtcaact ggataaaata atgatctccc
     1801 agggatcgag ttctttgact gtggacccgg gaaccacagg aatagccaca attggtgatc
     1861 tgatgaatat catcaacagc tgcggcctgc cgatacaggc gtcgatcaat gaaaatcata
     1921 agggcctgca ggttgaatcc actgtaatcg gccggacgct cctgatcgag gattcgggca
     1981 cgtcggatac tgcttataac ctgggtattg aaggctctcc cgatgtgctc ggcaatctca
     2041 tattcctggt ggacgccatt cgaaataacg ataagaaacc aatcggcaag gtcatcgcaa
     2101 gcctggactc atccataaat catattctta atgaacgtgc atcgatgcag gcaaaaatga
     2161 taagactgca taccgccgag tctcgtctga tggactacag cctggagtta accaaactgc
     2221 tgagtgaaac cgaaggtgaa gatttgagcg agctggcagc cgatcttgca tctcatgaac
     2281 atatcttctc ggctgcactg aattcggccg ccaggatcat acagccgtca ttgatggatt
     2341 ttataaggta aggttattat gaaaattgca actttcagat tcggtgaatt ggagataccg
     2401 gaagaaaatg ttatcgagtt tcccaagggg ttaataggtt ttgagcaatt taaacaattt
     2461 gttcttctgg aacgaaggga ctccgagcca tttcgctggc tgcagtccct cgaggatcca
     2521 gaccttgcct tcgtggtggt caacccggtg atattcttca aatattacaa aattgaggtt
     2581 cattataagg aactggagga tattcaagta tcgacactgg atagaactca aatctttacg
     2641 gttatcacca taccggatga tatttcgcgc atgtcggcaa acctgctggg gccgctggta
     2701 ataaatcttg acaacaactg cggtaaacag atcgtgctga gcaacagcac ctatacaacc
     2761 cggcattatc tgctggatga attaaacaag ggtgcaaggg aactggcggg gaagcactcg
     2821 ctggcggtgc aaatttgagt ttaagttata attctgttta atgaatcttt tggaagcgac
     2881 tttttgatat cggtggtgag aaatacgaca gtcgagtgag ggatgattca aatgccaaag
     2941 aaaaaacaaa aaccgaaagc gaaagatata tctgcgcccg gcaggaagaa acgtcctgcc
     3001 gggaaggcta aatcggtaag ctcctcgggg ccgggccgca gtgaacaata caccgaggct
     3061 ctgaaaaaag cagaggattt tctggatagg ggaaagcatg aagaggctgt catagcggca
     3121 ggccggcttc tcacaccgcg gattcgcgaa aaagacccga cgttgttcct atcggctgcg
     3181 cgtatcagcg ccttcgctaa tgttaatcta agaaattata tac
//
LOCUS       DSEB01000427            2267 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1007663, whole genome shotgun sequence.
ACCESSION   DSEB01000427 DSEB01000000
VERSION     DSEB01000427.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1007663"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            19..636
                     /locus_tag="ENO07_07735"
     CDS             19..636
                     /locus_tag="ENO07_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01898.1"
                     /translation="MSKRLPMMLAFFAGMVIIVAFFSGHPTLTNFRSNMQVWVSIIGG
                     FTLLLGVVSITSVNYRRVRKLEKDWPYKLILLISLVVSGGFGIFQGTEAGTVYDWLFQ
                     NMSAPMMSSMFAMLAFFIASAAYRAFRARTVEATILLVVAMIVMLGRVPIGQVIYHRL
                     PEITEWIMNYPNLAVQRGIIIGAALGAASMSIRIILGIERTYMGR"
     gene            638..1489
                     /locus_tag="ENO07_07740"
     CDS             638..1489
                     /locus_tag="ENO07_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01899.1"
                     /translation="MNRYRIAEILTGIDRRIIYLSILLATIIPFLVGFPQPIYVSKES
                     RDLYNWVENIRDGSTILLTFDYYPSTLAEVEPMSRAAIKHCWRKDMKLICMTTVPLGG
                     PSITERVMNDMATNWAVEELGAKKIYGVDYINLGYKPDYKAVLMGMGTSFRDIYPTDF
                     RSVPLDSFPITKNVDKYSDVNFLYIISDNGIVDEWVTIANRQFGIPLGAGVTAVMAPK
                     FYSYLQSQQMVGLLGGMRGAAEYEKLIGSEGMATLFMGPQSLVHLMIIFFILLGNTAY
                     FLGGRKV"
     gene            1486..2133
                     /locus_tag="ENO07_07745"
     CDS             1486..2133
                     /locus_tag="ENO07_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01900.1"
                     /translation="MNPADSSLIEMSVAGFLILSIFSFLYKDNPFYRLAEHIFAGLTA
                     GYYVGLIYQTVIVQQLTIPIYREGKLELIFPALLGILMFTRFFDRFSYLSRISLAFVM
                     GNTAGIFLIQQLHGLVLPQTQATIINPLAGSWLLGAVPGGMIIIAGVITTLIYFYFSK
                     EHKGVLGKTARVGMVFIMISFGAHFGYTVMARLSLLIGQVKFVAFDWLPRLIGVN"
BASE COUNT          529 a          534 c          577 g          627 t
ORIGIN      
        1 agctaagtgc atgatttatt gagtaaacgg cttccgatga tgctggcctt ctttgcaggc
       61 atggtcatta ttgtcgcttt tttctccggc catccgacgc tgaccaactt ccgctccaac
      121 atgcaggtct gggtctcgat catcggcggt tttactttgc ttctgggggt ggtttcaatt
      181 accagcgtga attacaggcg tgtccgaaag ctcgaaaaag actggcccta taagctgatc
      241 cttttaatca gcctggtggt atcgggaggt ttcggtatat ttcagggtac cgaagccggt
      301 acagtctatg actggctttt ccagaatatg tccgctccga tgatgtcctc catgtttgcc
      361 atgctggcgt tctttatagc atccgccgcc tatcgcgcct tcagggctcg tacagtggag
      421 gcgaccatcc tgttggtagt agctatgata gtgatgctgg gaagggtgcc gatcggccag
      481 gtcatatatc accggttgcc cgaaatcacc gaatggatta tgaattatcc caatcttgcg
      541 gttcagagag gaatcataat cggggccgct ctgggcgcgg caagtatgtc gatcaggatt
      601 attctgggga tcgaaagaac ctacatgggc agataatatg aacaggtata gaatcgccga
      661 aatactcacc ggcatagacc gcagaattat ttatctgagc attttgcttg cgactataat
      721 tccgtttctg gtcggttttc cacagcccat ttatgtcagc aaggaaagcc gcgacctgta
      781 caactgggtc gaaaatatcc gggatggcag caccatcctt ctgaccttcg attactatcc
      841 ttcgaccctg gccgaagttg agccgatgtc acgcgccgca atcaagcact gctggcgcaa
      901 ggacatgaaa ctgatttgta tgaccaccgt gccgttgggg gggccaagta tcaccgaaag
      961 ggtcatgaac gatatggcca caaactgggc ggtggaggag ctgggcgcga aaaaaatata
     1021 tggtgtggac tatataaacc tcggctacaa gccggattat aaagcggttt tgatgggtat
     1081 gggaacttca ttccgggata tctatcccac cgatttccgc agcgttccgc tcgattcatt
     1141 cccgattacc aagaatgttg ataaatattc cgatgtcaat tttctctata taatttcgga
     1201 taacggcata gtcgatgaat gggtaaccat cgccaaccgc caattcggca tcccgctggg
     1261 cgcgggagtt accgctgtga tggcgcccaa attctattct tatctccaga gccagcagat
     1321 ggtggggctt ctgggtggca tgcggggcgc agccgaatat gaaaaattga tcggttcgga
     1381 gggtatggcc actctcttta tgggcccgca gtcgcttgtc cacctgatga taatcttttt
     1441 cattctgctg ggaaatacag cctattttct ggggggacgc aaggtatgaa cccggccgat
     1501 tccagcttga ttgaaatgag tgtggcggga tttttgatcc tatcgatctt ctcatttcta
     1561 tataaggaca acccgttcta tcgcttagca gagcatatct tcgccggatt gaccgccgga
     1621 tattatgtcg gtctcatcta tcagacagtg atagttcagc agctaaccat tccgatttac
     1681 agggagggca aactggaatt gattttcccg gccctgcttg gaattctgat gttcacccgc
     1741 tttttcgacc gattctcata tctttcacgt atttcactcg ccttcgtaat gggcaatacg
     1801 gccgggattt tcctgataca gcagcttcac ggcctggttc ttcctcaaac ccaggcgaca
     1861 attataaacc cgctggcggg aagctggctc ctgggagccg tcccgggcgg tatgatcata
     1921 attgcgggtg taatcaccac attgatatat ttctactttt ccaaagagca taaaggggtg
     1981 ctgggcaaga ccgccagggt cggaatggtg tttatcatga tatcgttcgg ggcgcatttt
     2041 ggctataccg ttatggccag gttgtcgctc ttgatcgggc aggtaaaatt tgtcgctttt
     2101 gactggctac caaggcttat cggcgtaaat tagttgcttt ttttcggaat atccatttat
     2161 taagctactt aaaaataaat ctttaatcca attgtatgtc gaaagggtga aattgtgaaa
     2221 gtagtaattc ccgtagccgg tatcggaacg agactgcgac cccacac
//
LOCUS       DSEB01000428            2713 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1005137, whole genome shotgun sequence.
ACCESSION   DSEB01000428 DSEB01000000
VERSION     DSEB01000428.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1005137"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(166..2508)
                     /locus_tag="ENO07_07750"
     CDS             complement(166..2508)
                     /locus_tag="ENO07_07750"
                     /inference="COORDINATES: protein
                     motif:HMM:PF09397.8,HMM:PF13491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDS01901.1"
                     /translation="MKALANLSPRRKDEFLGLLMLTLGALIFFSLVSHSQHDDISLSE
                     NRGFDIFEILPDNWVGLVGAMLSHLLYYLLGMLAFFLPVFALLTGVKYLFRWKLPRLR
                     KKIFYTFLISSALALVLSVKYVDAGGHVEFVSTPGGALAVGVASFIIRFFGKVGSFLL
                     FFSAIIILLFLLIEWRPSWWEKKITSIPERVRNWMLALFGIGAATKKAKSSRAKSAKQ
                     KRFSLPRVFRSVSVALKRVEGIFRRAPIREKEQETGDDFEAEEGRQGKFTDLFEQLSY
                     EKPADTRVEKVSEESQEKEKLAIKMTRKSEDGYQAPDFGILDANPQPDQAITNAELKM
                     VSSQLLDTLKTFHIEVVDDRIETFPGPVITRYEFKPAPGIKVNQVINLSDDLALALQA
                     KRIRIIAPVPGKAAIGVEIPNRHPQTVYFKDVVTDQKFKSNKILLPMALGLTTAGEPY
                     VADLASMPHLLIAGATGSGKSVCMNVIVSSLIFRHHPRNLRFIFIDPKMLELSVYSGI
                     PHLERPVITKPKQAERVLNDAVVEMENRYKRLASKGVRNIVQYNNKVQASERLPYLVI
                     AVDELADLMMSQSSSRIEILLTRLAQMARAVGIHLILATQRPSVDVITGLIKANFSAR
                     IAFQVATKIDSRTILDGNGAEKLLGRGDMLFLEPGQAEPVRVHGALISSEETGRLVEI
                     LKTQEVEVRKIDSMTEEAVKQRALVSDADPLFKEAAEMVIRHKQGSVSLLQRRLGIGY
                     QRAARLIDLLEAKGVVGPYDGSKAREVLADQTYLEKLRNN"
     gene            complement(2608..>2713)
                     /locus_tag="ENO07_07755"
     CDS             complement(2608..>2713)
                     /locus_tag="ENO07_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016114824.1"
                     /note="histone-like DNA-binding protein; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein HU"
                     /protein_id="HDS01902.1"
                     /translation="ARTGRNPQTGQPIKIAAARIPKFKAGKKLRQAIK"
BASE COUNT          649 a          755 c          624 g          685 t
ORIGIN      
        1 acctgcaggg tctcaatatc ctccaaccgc ctgtcaatcc tgctcaatac ctcttcctct
       61 ttcgtggcca ggagagaggc ggaaagcagg aagataaatg cggccgaaaa aattatggtt
      121 tgtgtcaact ttttcataat acttcttata caatccagat ggggatcagt tgtttcttaa
      181 tttttcaaga tatgtctgat cggccagcac ctcacgcgct tttgaaccgt cataaggccc
      241 cacaactcct ttagcctcaa gcaaatcgat cagccgggcg gcccgctgat agccgattcc
      301 gagacgtctc tgtaaaagcg aaactgaacc ctgtttatgg cggatgacca tctccgccgc
      361 ttccttgaaa agcggatcgg catcagaaac cagggccctc tgtttgacag cctcctcagt
      421 catagaatct atctttctga cttcaacttc ctgggttttc aatatctcca ccagacggcc
      481 ggtctcttcc gacgatatca gggcgccgtg gaccctgacc ggttccgcct gccccggctc
      541 aagaaagagc atatcgccgc gccccaggag tttctccgcg ccgttgccgt ccagaatggt
      601 gcgggaatct atcttggtcg caacctgaaa tgctatcctg gccgagaagt tggccttgat
      661 aagcccggtt atcacatcca ccgagggcct ctgggtggcc aggatcagat ggatacctac
      721 agccctggcc atctgtgcca gacgagtcag caaaatttca atccggctcg acgactgcga
      781 catcatcaga tctgccagtt cgtcaacagc tatgacgaga taaggcagcc tttcagatgc
      841 ctgcacctta ttgttgtatt ggacgatatt gcggactcct ttggaggcca gtcttttgta
      901 acggttttcc atttccacca cggcatcgtt gaggacccgc tccgcctgct tgggcttggt
      961 gatcaccgga cgttccaggt gaggaatccc cgaataaacc gaaagctcca gcatcttggg
     1021 atcgatgaat atgaagcgca ggttgcgggg atgatgcctg aatatcaggc tgctgacgat
     1081 gacattcatg cagaccgatt tgccgcttcc ggtcgcaccg gcaatcagga gatgaggcat
     1141 ggaggccagg tcggctacat atggctcgcc cgctgtggtc aggcccagcg ccatgggaag
     1201 caaaatcttg tttgatttaa atttttgatc agtgaccaca tctttgaaat agactgtctg
     1261 gggatggcgg ttgggtattt ccacaccgat cgccgccttt ccgggaacag gcgcgataat
     1321 cctgatcctc ttggcctgca gcgccagggc gagatcgtca gaaaggttta ttacctgatt
     1381 gactttgatt cccggcgcag gcttgaattc ataacgggtt atcaccgggc cgggaaacgt
     1441 ctctatcctg tcatccacca cttctatatg aaatgttttc aatgtgtcca gtaactgcga
     1501 acttaccatt ttcaattcgg catttgtaat cgcctggtcg ggctggggat tggcatcgag
     1561 gattccaaaa tccggcgcct gataaccgtc ctcggacttg cgcgtcatct ttatggcaag
     1621 cttctctttt tcctgactct cctcgctgac tttttccacc ctggtatcgg ccggcttttc
     1681 atatgaaagc tgctcaaaca gatcggtaaa cttaccttgt cttccttctt ccgcttcaaa
     1741 atcatcaccg gtttcttgtt ccttttctcg aatcggtgcg cgcctgaaga tgccctcgac
     1801 gcgcttgagc gcaacggaca cggatcggaa gaccctgggt aaactgaagc gcttttgctt
     1861 tgccgattta gccctggagg atttcgcctt ttttgttgcc gcgcctatcc cgaaaagcgc
     1921 cagcatccag ttccgcaccc gctccggtat agaggtgatc tttttctccc accacgacgg
     1981 gcgccactct atcaataaaa acagcaggat aataatcgcg ctaaaaaata aaaggaatga
     2041 acctactttt ccaaaaaacc tgattataaa actggccaca ccaaccgcca gggcccctcc
     2101 cggtgtggaa acaaattcta catgtcctcc cgcatcaaca tacttcacac tcaataccag
     2161 agccagcgcc gaggaaatca aaaacgtata gaatattttc tttcttaatc tcggcagctt
     2221 ccagcgaaat aaatatttca cacccgtaag cagggcgaat acaggcagga aaaacgccag
     2281 catgcccagg aggtagtaca gcaaatgcga cagcattgcc ccgaccagac cgacccagtt
     2341 gtcgggcagg atttcgaata tatcaaagcc ccggttttcg ctcaaactga tatcgtcatg
     2401 ctgggaatgg gatactaacg aaaagaaaat cagagcccca agggtcaaca tcaaaagacc
     2461 caggaactca tctttacgcc gtggtgataa atttgccaaa gccttcatac actcaccaaa
     2521 atacttatct tttagccctc gatcaacaac taatttgaaa aggcagcccg taccgcggga
     2581 ctgccttttc aatctttttt cccgcgatta cttgatcgcc tgtctcagct tttttcccgc
     2641 tttgaacttt ggaatccttg cggcagcaat cttgatcggc tgccccgttt gcgggtttct
     2701 accggtacgg gcc
//
LOCUS       DSEB01000429            2058 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1009460, whole genome shotgun sequence.
ACCESSION   DSEB01000429 DSEB01000000
VERSION     DSEB01000429.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1009460"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..488)
                     /locus_tag="ENO07_07760"
     CDS             complement(<1..488)
                     /locus_tag="ENO07_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01903.1"
                     /translation="MKDCLFQMRLKLLPLAILLLLAAPVFAQVPDQSGEASLEADTLQ
                     EEPQIPPPAPPEIVEIKDSDNDHGHSIDLEWELSPDDDGTGNYVTGYEIWRGEMVDGK
                     IEFKLRRTVPYGVKIYTDRGDAAGGVDPLPNGKDYWYKIAAVADEDAKTESEVYGPVQ
                     ATG"
     gene            complement(512..1279)
                     /locus_tag="ENO07_07765"
     CDS             complement(512..1279)
                     /locus_tag="ENO07_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01904.1"
                     /translation="MNPKTIKWIIFVIVLSAIVLSVYFVSLDRGSFRDGVFVTTAAFL
                     TLFIMSFLYKDNPYYKFAEHLFVGVSAAYWMTMGFWGTMVPNLVAKLWPGSVRGVLPA
                     LADKDPSLWFLIPLFLGIVLLMRLSPKGGWISRWALAFIVGTTAGLNLIRYLRSDFIQ
                     QVNNTILPLVDKTEAGISFWGSFSNIFIFVGVLCGLVYFFFSKEHKGFFGGAARIGIL
                     VLMLTFGAGFGYTVMARISLLAGRMTYLFGDWLGLIK"
BASE COUNT          556 a          560 c          474 g          468 t
ORIGIN      
        1 ccggtagcct gcaccggtcc gtaaacctcg gattctgttt ttgcatcttc atcggctaca
       61 gcagcgatct tgtaccagta gtctttgccg ttgggcaaag gatcaacccc gcccgcggca
      121 tcgccgcgat cggtataaat tttgactccg taggggacag ttcgccgaag tttgaactcg
      181 attttaccat cgaccatctc gccgcgccag atctcgtacc cggttacata atttccggta
      241 ccgtcatcat cgggagaaag ctcccactcg aggtcaatcg aatgtccatg gtcattgtct
      301 gaatccttga tttccacaat ttcaggtgga gcgggtggtg gaatttgagg ttcttcttga
      361 agcgtatctg cctccaggga agcctcacca gattgatccg gcacctgagc aaatacaggc
      421 gctgccagaa gcaacagtat tgccaggggc agtaatttca atcgcatctg gaaaaggcaa
      481 tctttcataa gcacctctaa taaattgtta attatttgat taatccaagc caatcgccga
      541 aaagataggt catcctcccg gccaaaagtg atattcttgc cataacggtg tagccgaatc
      601 cggctccgaa ggtcaacatc aggaccagga tgccgatacg cgctgcgccc ccgaaaaacc
      661 ccttgtgctc cttggaaaag aagaaatata ccagcccgca gagcacgccc acaaatataa
      721 atatgttgga aaaactgccc cagaaactta tcccggcctc agttttgtcc actaatggaa
      781 ggatggtgtt attgacctgt tgaataaaat cggaacgaag ataacggatc aggttcaaac
      841 ccgcggtggt tcccacaata aaggccaggg cccagcgcga aatccagccg cctttggggg
      901 acagacgcat cagaagtaca atccccagga aaagcggaat caggaaccag agactgggat
      961 ccttgtcggc caatgcgggc agcactcccc tgaccgagcc cggccagagt ttggccacca
     1021 agttcggtac catagtaccc cagaaaccca tcgtcatcca gtaagccgca gatactccca
     1081 caaaaagatg ctccgcaaat ttataataag gattgtcttt gtacaggaag gacatgataa
     1141 acaaagtaag aaatgctgcg gtggtgacaa aaacgccatc ccggaatgag cccctgtcca
     1201 gactcacaaa atatacactc agcacaatag cggagagtac gattacaaat atgatccact
     1261 taatggtctt tggattcaca gtccttattt ctccagagtc aatttgcgtt ccttaccgcg
     1321 atacatgaag tacatcacat ttccaaaaac gataaagagc ataatcacaa tatgcgcaac
     1381 catctgcgca accatcatat tcatgcccgg cgtgggggtc ttcgcgaatg cctccggata
     1441 tgtgttcagc acgtgactct catattcggc cgcgcccttg atcccgccta ggagccctac
     1501 catctgattg ggataatagg gataaagctg gggggcggac actgctgtgc aggcgcctcc
     1561 gactggaatt ccgcccgggt cgccagcgaa taaaatccac tccttgatac cagggaatcc
     1621 gccgccgaaa gacacaatca aatccatatc cttgagactt gacacctgct tcatgatggg
     1681 aatttcttca atatccgtgc cgttggcatc ggtgctgtac atgcgcttca tatcgtccac
     1741 aatcacacgg atcagcccct ggtttccggc tttatatccg agattgacat agtctattcc
     1801 atattcatag tccggaaatt ccggcatcaa cacctgttcc acagtctgaa ccgtaaggct
     1861 ctgccctgtg gcccagaccg ccataaggta aactttcaga tccttcaaac aggcgtgacg
     1921 caggataaca tcaaccatag gctgaatttc aggcgcggta gtcgggccgt aatcgaacga
     1981 catcaataga cgcgagccgg gaggcatttc atctattttg tcgtacaacg ccaggactat
     2041 gggcgagggc tcttcctc
//
LOCUS       DSEB01000430            2070 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1009343, whole genome shotgun sequence.
ACCESSION   DSEB01000430 DSEB01000000
VERSION     DSEB01000430.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1009343"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..>2070)
                     /locus_tag="ENO07_07770"
     CDS             complement(<1..>2070)
                     /locus_tag="ENO07_07770"
                     /inference="COORDINATES: protein
                     motif:HMM:PF01565.21,HMM:PF13534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDS01905.1"
                     /translation="EAYASDASIYKAYPRAVLIPTTYRQLRNAVNVLRKHGLAFTPRG
                     GGSGLAGGAVSDSVLIDFSRMNRVLSCDLHARTVDVECGIVYDRLNRYLLGKGLFFPP
                     DPSSGDTCQIGGMLGNNSSGARSVKYGTTRKYVQELHILLPDGRELIARDFKIGSKEI
                     EQLFLEYPQLGNIYRLISGNRDEILKAYPRLRKNVCGYDLRTMVEKLDQGIFALPQLF
                     IGSEGTLGLFVSAKLRLLPLPGEKLTSLILFEKLDQVGDAALDFLRLEPAGLELIDGN
                     TLDLIGRERFDLPPSAEAMLIVEFDDPPFDEKVSALKDYLKKYSLSSEPVFETDPKKQ
                     AALWGARKAIVPTLYRLDKNARPWGFIEDAAIPSEKMPEFIRFLNRIFNENGLTCGIF
                     GHIGDGNLHVRPAINLASEGGQRLARSLFDRVYDRIFELGGSATAEHGDGHLRAEVIP
                     RMYGDKIYNLFLRIKEQLDPQYRSNPEVLLSKRNFLENIDFEKVVRECAACGKCNNYC
                     PSFEVHKSENMAARGWVRVMLAENFSRRDAKKELAGCLNCKSCLIVCPAGVDVSRYII
                     QRRSEHPGFWGKQIFGLMRNQKRFEEIAGFSGGLLRLFDNRAMRPLLELASMPFARID
                     RERILPSFTKKTLRQKYPQLASDNGGDVAYFYGCADNLLDSNTGPSLINVLQKHGFSL
                     SLPEQKC"
BASE COUNT          503 a          572 c          506 g          489 t
ORIGIN      
        1 cagcatttct gctcgggcaa tgaaagcgag aagccatgct tctgaagcac atttatcaga
       61 gatggaccag tatttgaatc gagcagatta tctgcgcaac catagaaata ggcgacatct
      121 cccccgttat cacttgcaag ctgagggtat ttttgccgca gcgttttctt tgtaaacgag
      181 ggcaggattc tttctctgtc aattcttgca aagggcatcg aggccaattc cagcaacggc
      241 cgcatggctc tattatcaaa caaccgtaga agcccgccgc tgaatccggc gatctcctca
      301 aatcgttttt gatttcgcat caggccgaaa atctgcttgc cccagaagcc gggatgttca
      361 ctgcgccgct gaataatata ccgcgagaca tcaacccccg cggggcagac gatcaaacag
      421 gatttgcagt tcaagcaccc cgccagctcc ttttttgcgt ccctgcggct gaagttctcc
      481 gcaagcatga cccgcaccca tccccgggcg gccatattct cacttttgtg gacttcaaaa
      541 ctggggcagt aattattaca ttttccgcag gcggcgcatt cacgcacgac tttttcaaaa
      601 tctatatttt ctaggaaatt gcgtttagac aataaaacct ctggattcga cctgtattgg
      661 ggatcgagct gctccttaat ccggagaaag agattgtaga tcttatcgcc atacattctg
      721 gggataactt ccgcccgcag atggccatcc ccgtgttcgg cggtggctga cccgcccagc
      781 tcgaaaattc tgtcataaac ccggtcaaaa aggctcctgg cgagcctctg gcccccctct
      841 gaggcgagat tgatggccgg gcggacatgc aggttgccat caccgatatg accgaatata
      901 ccgcatgtga ggccgttttc gttaaaaatc cgatttagaa accggataaa ttcaggcatt
      961 ttttctgagg gaatggcggc atcctcgata aagccccatg gacgcgcgtt tttgtcgagc
     1021 cggtataaag tcggcacaat cgccttgcgg gccccccata atgctgcctg tttcttcggg
     1081 tcggtctcga acaccggttc gctcgataaa ctatatttct tgagataatc tttcagcgcg
     1141 gaaacctttt catcaaacgg cggatcatcg aattcgacaa tcagcatcgc ctcggccgag
     1201 ggcggcagat caaaacgctc ccgaccgatc aggtcaagcg tattgccgtc gatcagctcc
     1261 agcccggccg gttcgagcct gaggaaatcc agggcggcat cgccaacctg atccagtttc
     1321 tcaaatagaa tcaaggaagt aagcttctct ccgggaagag gcagcaatct caactttgcc
     1381 gatacgaata atcccagggt cccctctgag ccaataaaca actgaggcag agcgaatatg
     1441 ccctggtcca gtttctccac catcgtcctc aggtcatacc cgcagacatt tttcctgagc
     1501 ctgggatagg ctttcagaat ctcatctcta ttcccggata tcaggcgata aatattccca
     1561 agctgcggat actccaaaaa cagctgctct atttcttttg aaccgatttt gaaatcgcgt
     1621 gcgatcagct cgcgtccatc ggggagaagt atatggagtt cctgcacata ctttcgagta
     1681 gtaccatatt ttacagatct tgcacccgag gagttattgc ccagcatgcc ccctatctgg
     1741 caggtatcgc ctgaagaggg atcgggcgga aagaaaagcc ccttgcccag aagatagcgg
     1801 ttcagacggt catagacaat tccacactcg acgtcgacag tccgggcgtg aaggtcgcag
     1861 gagagcacgc gattcatcct ggagaaatct attaagacgc tgtcggatac agcacccccc
     1921 gcaagccccg aacctccccc gcgcggggta aatgccagac cgtgtttcct gagcacgttt
     1981 acggcgtttc tgagctgtct gtaagttgtc ggaatgagta cggcccgggg ataggccttg
     2041 taaatggagg catcggaggc ataggcctcc
//
LOCUS       DSEB01000431            1501 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1019084, whole genome shotgun sequence.
ACCESSION   DSEB01000431 DSEB01000000
VERSION     DSEB01000431.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1019084"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            397..597
                     /locus_tag="ENO07_07775"
     CDS             397..597
                     /locus_tag="ENO07_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007294878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDS01906.1"
                     /translation="MVEGTVKWFNESKGFGFIQQDNGGDVFVHHTAIQGQGFKTLAEG
                     ERVKFEVIEGKKGPAAENVVSL"
BASE COUNT          392 a          367 c          345 g          397 t
ORIGIN      
        1 gtcgtgtatc ttatattcag aattaagtaa gaagtagcaa tatttttacc tgtcacgcct
       61 accttccgcc aaagatatcg gcatgatctg gtcctgaaag ttttgccaat tacttagaaa
      121 aaatgtaagc tggtgacctt ttataatcga tacaatcggg actgatcaaa aataatttac
      181 caatctacga cgattttatc tttagagcgg atatacgaat ctcacatttt aatcaatcat
      241 ccataaagtg aaaacaaaga gttttcctga tattttatca ggatttataa ttcagcccgg
      301 atgaccggtc tgagcgtcag gcagaatgtt tctggctggc acatttttta acaatggttc
      361 tcatagagaa ccagcagtac ttaggagtat gcagtaatgg tagaaggaac agtaaaatgg
      421 tttaacgagt cgaaaggatt tggttttatt caacaggaca acggtggcga tgtattcgta
      481 catcataccg ctatccaggg ccagggcttc aagacactcg ctgaaggcga acgtgtaaaa
      541 tttgaagtga tcgagggcaa gaaaggaccg gcagcagaga atgttgtaag cctttaaact
      601 cgacctctgt ttgatttcaa aaggccccgc acttgtgcgg ggcctttttt attttaagga
      661 gcagccggat tatcttcttt catcaacagg atctggagag ccgccgtcga aggttcagtg
      721 cagcgcagga gcacattaag cgcatttcct ccgctttcgg ttgtaccgtc aataaacacc
      781 agcatatttt tgtcatcatt caaggcccca tgggtcagcg gattaccgtc gatcaatgtt
      841 tcacctccgg acgaaatcgc tatattcccc tgggagatct cgccggattg agtaggactg
      901 gtccagtccg cttcgtactg cccctggctg ttgaccgata atttcccgta accccaccga
      961 ggatcatccg gcggattatc cccgctcacc acatgatacc cgcaccataa tcctgcgaga
     1021 tctactgcac tgaatgtaac cgcaggatct ctcttcagca ttatggtaag agcgtttcca
     1081 tcgttctggg cagtgccgtc tatgaaaacg atcatatctt tcgcatcatt catcacacca
     1141 tgggtcaatg gctgcccatt tatggtaatc atgccggagc cattgatctg aatcgatccc
     1201 tgaacagttt cgctgggtgt tccaaggctt gtccatatgg aggtgaatcc acccacactg
     1261 tcgatatccg ccgtgccgta tccccagcgc ggctcatcag cgggagcatc gccactgaca
     1321 atctggtgac cgtaccaggt gccggcaagg tcggccgcag caaagatctg gcccgacttg
     1381 cgccggatca gcacaaccag tccgttgccg gaatttgaca gagttccgtc aataaaaacg
     1441 attagttccc tgtcctggct catcacaccc tgggtgagcg gatcattgtt gacggtgata
     1501 a
//
LOCUS       DSEB01000432            1766 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1013330, whole genome shotgun sequence.
ACCESSION   DSEB01000432 DSEB01000000
VERSION     DSEB01000432.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1013330"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..640)
                     /locus_tag="ENO07_07780"
     CDS             complement(<1..640)
                     /locus_tag="ENO07_07780"
                     /inference="COORDINATES: protein motif:HMM:PF02811.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDS01907.1"
                     /translation="MEFNHPVGDYHVHPDFSIDAEGTLREYCQKAIELGLAEIVFTTH
                     VDSNPASEDWHKIIIDGRKQPVTTDNLKIYQDAVTELISGNDPVPIAVRCGVELEYHP
                     EVKDSFLKMVADLNFDFVLGAVHFVDGALLTIEEDVQRLLDKYSAVELIEEYCKIVQQ
                     ACQIKIFDSLAHLDIYRRIGHKLFPEEAARIDYEFLDETLEQLVKCGLPIEVN"
     gene            complement(645..1241)
                     /locus_tag="ENO07_07785"
     CDS             complement(645..1241)
                     /locus_tag="ENO07_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000465472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDS01908.1"
                     /translation="MEVNCYLVYETESCKGVIVDPGDEGSHIMHRIEDLELKIDQIVL
                     THGHGDHIGAVEYIQRRLGAEVAIGRADADMLSDASRNLSENLGLSIKLRQPDLLLSE
                     GDTIAVGDHKLTVIETPGHTLGGISLVGEGFVISGDLLFAGSIGRVDLPGGNYETIIN
                     TIKTKILPLGDGYVIYPGHGPKTSVLQEKTFNPFLIEE"
     gene            complement(1356..>1766)
                     /locus_tag="ENO07_07790"
     CDS             complement(1356..>1766)
                     /locus_tag="ENO07_07790"
                     /inference="COORDINATES: protein motif:HMM:PF00561.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDS01909.1"
                     /translation="SARGLPVRGNVNDKDMVLRLYLSQFSDPSVVSADEREKIAELAV
                     SENVPGCTHKILQANGDFSVAGIENIKVPTLIIWGEKDPVLPKIMAENFANKIPNSQY
                     VMIPEAGHLPHEESPEDFNTVLLDFLKGNLPKSG"
BASE COUNT          417 a          478 c          418 g          453 t
ORIGIN      
        1 tatttacttc aattggaagg ccgcatttga caagctgctc cagtgtctca tccaggaact
       61 cgtaatcgat ccgggccgcc tcctccggaa acagcttatg accgatccgt ctatagatgt
      121 ccagatgtgc caggctgtca aatattttaa tctggcaggc ctgctggacg attttacagt
      181 actcttcaat caactcaacc gcagaatatt tgtccagaag acgctgcaca tcttcctcaa
      241 tcgtaagcag ggcgccatca acgaaatgaa ccgcacccag aacaaaatcg aaattcaggt
      301 cggcgaccat cttcaggaag gaatccttga cttccggatg gtattccagc tccacaccgc
      361 agcgcaccgc aatcggcaca ggatcattcc cactaataag ctcagtgacc gcatcctgat
      421 atattttcag gttatcggtt gtaaccggct gcttccggcc gtctatgatg atcttatgcc
      481 agtcctcact ggcgggattg gagtctacat gggtggtgaa tactatctca gccagaccca
      541 gttctatcgc tttctggcaa tattccctga gcgttccctc ggcatctatc gaaaaatcgg
      601 gatgtacgtg gtaatctcct actggatgat tgaattccat tttgctattc ctctatcaag
      661 aatgggttga aggttttttc ctgcagtact gatgttttgg gtccgtgtcc gggatagatc
      721 acataaccat ccccgagcgg aagaatcttc gtttttatag tattgatgat agtctcataa
      781 ttgcccccgg gaaggtccac gcggccgatc gaccctgcaa aaagcaggtc gccgctgata
      841 acgaagccct cgcccaccag gctgatcccc cccagcgtat ggccgggcgt ctcgatcacg
      901 gtgagtttat ggtctcccac ggctatggta tcgccctcgg agaggaggag atcgggctgg
      961 cgaagcttaa tcgagaggcc cagattctcg gagagattgc gggaggcatc gctgagcata
     1021 tcggcatcag ccctgccgat ggcgacctct gcccccaatc ttctctggat atattccacc
     1081 gctccgatat gatcaccatg cccgtgagta aggacgatct gatctatttt gagctccaga
     1141 tcttcgatac gatgcatgat atgcgagcct tcatcgccgg gatcgactat aacgccctta
     1201 catgattcgg tctcgtaaac caggtaacag tttacctcca tcatgccgac cggcaactgc
     1261 tccactatta tgtcattgaa ttcatcagcc ataagccact attatagaag ctaatgggtg
     1321 ttatgtcaaa cagatttgat gattgaggaa ctgtctcagc cggatttagg cagattgccc
     1381 ttcaggaaat caagcaatac tgtgttgaaa tcctcggggc tttcctcgtg gggaagatgg
     1441 cccgcttcgg ggatcatgac atattgggag ttcggtatct tgttggcaaa attctcggcc
     1501 ataatcttcg gcagtaccgg gtctttttcg ccccagatga tcaaagtagg aaccttgata
     1561 ttctcaattc cggccacact gaaatcaccg ttggcctgca gaatcttatg agtacatccc
     1621 gggacatttt cgctcaccgc aagttcggca atcttctcgc gctcatcagc ggacaccact
     1681 gacggatcgc tgaactggct caggtaaagc ctgagaacca tgtccttgtc attgacgttg
     1741 cccctgaccg gcagtccccg ggccga
//
LOCUS       DSEB01000433            2359 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1007018, whole genome shotgun sequence.
ACCESSION   DSEB01000433 DSEB01000000
VERSION     DSEB01000433.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1007018"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            52..1590
                     /locus_tag="ENO07_07795"
     CDS             52..1590
                     /locus_tag="ENO07_07795"
                     /inference="COORDINATES: protein
                     motif:HMM:PF03572.16,HMM:PF14684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01910.1"
                     /translation="MSEREVQIPVRPGDYDGLEAIGGRIYYFSNPFRGLSGPKGDQKR
                     ELRYFDMEKKKDQLFMEDVAGFGISADHSKILIKAGNKYIINDASAEKGDQTSGVLDL
                     SGMDMYLDRRAEYREMFDDAWRRYRDFFYDSNMHGVDWQAMYELYKPLVKYASHRYDL
                     TYIIGELAGELACSHTYVGGGDSYEPESDKVALLGVEFEADTSVNLFQIGKIHDGKNW
                     EERLRSPLTEPGIRAEQGYYIFEIDGQRLTAEMNPYSLLVNKADRIVTLKISPTADEQ
                     DAYEVEVDPIDNEQPLWYYNWVENKRAIVDSLSDGRIGYIHIPDMGSFGLNQFARQFY
                     HLYKKEGLIIDVRFNGGGFVSQLALDRLRRVVVGMGAGRHDWVGTYPSVAFHGHMACL
                     INQYSCSDGDIFPYYFREYGLGPLIGMRTWGGVIGIGGFRPLMDGGYVTVPGGGSFSL
                     EGEWIMENVGVEPDIEIEQDPALLMQGRDPQLERAVEYVMEKIRTEPKTLPDKPGPPD
                     ERKR"
BASE COUNT          623 a          505 c          647 g          584 t
ORIGIN      
        1 caagataaga gaaaagagct cccatcaaaa ataaatattg atattgaggg attgtctgaa
       61 cgtgaggtac agattcccgt tcggcccggc gactatgatg gcctggaagc tataggcggc
      121 aggatatatt atttttccaa tccttttagg ggtctatccg gccccaaagg tgaccagaaa
      181 agggaactcc gttactttga catggaaaaa aagaaggatc aactctttat ggaggatgtt
      241 gccggattcg ggatttcggc cgaccacagc aaaatcctga taaaggcagg gaataaatat
      301 atcattaacg atgcctcagc ggaaaaaggt gatcagacat ccggagtgct ggatttgtcg
      361 ggcatggata tgtatctcga tcgcagggcg gaataccggg agatgttcga tgatgcctgg
      421 aggcgctacc gcgatttctt ttacgattcc aatatgcatg gagtcgactg gcaggcaatg
      481 tatgaacttt ataaaccgtt agtgaaatat gcctcgcacc gttatgacct gacttatatc
      541 atcggcgagt tggccgggga gttggcctgc agccatacct acgtgggcgg cggagattct
      601 tatgaacccg agtccgacaa agttgccctg ctgggagtcg aatttgaggc ggatacctcg
      661 gtcaatctct ttcaaattgg taaaatccat gatggtaaga attgggagga aagattacga
      721 tcgccgctga cagagccggg catcagagcc gagcaaggat attatatttt tgagatcgac
      781 ggccaaaggt tgacagcaga gatgaatccg tacagcctgc ttgtgaataa ggctgataga
      841 atcgtgactc ttaagataag tccaacggcg gatgaacaag atgcttatga ggtcgaagtg
      901 gatccgatag ataatgaaca gcctttatgg tattataact gggttgaaaa caagcgtgct
      961 attgtcgatt cgttatctga cggccggata ggttatattc acatcccgga tatgggctca
     1021 ttcggcttaa accagtttgc ccgccagttc tatcatctct ataagaaaga aggcttaatc
     1081 attgatgtgc gctttaatgg tggcggtttt gtatcccagc ttgcccttga tcgtctaagg
     1141 cgtgttgtgg tcggtatggg cgctggacgg catgactggg tgggtacata tccgagcgtg
     1201 gccttccatg gtcatatggc ctgtctgata aatcaatatt cttgttccga tggagatatc
     1261 ttcccttact atttccgcga gtatggtctg gggccgttga ttggtatgcg tacatggggc
     1321 ggtgtgatcg gcatcggcgg atttcggccg ttgatggatg gcggttatgt gactgtgccc
     1381 ggtggcggaa gtttcagcct ggagggtgaa tggatcatgg aaaacgtggg tgtggaaccg
     1441 gatattgaaa tcgaacagga tcccgcgctt ctgatgcagg gacgcgaccc gcagctggag
     1501 cgcgctgtcg aatatgtaat ggagaagatc aggaccgaac caaagactct gccggataaa
     1561 ccgggcccgc ccgatgaaag aaagcgataa tagttcccgg aggctgatat ttaatgaaat
     1621 gtactcaaaa catgttattc tgagcgtagc gaagaatctc taatgaatca aatagattct
     1681 tcggtccgta agtggccgga tttacaatcg ctgcgctccc tcagaatgac agcttacaga
     1741 attgtctatg aaagccgtcc tgccggctaa aatccctggc agggcggctg gccgtcacgg
     1801 aagatatagt tgaccagata gatcacatca acaagcttga ccaccccgtc acaatcagca
     1861 tcgccgagct catacggatc agggacggcg ccattgagga agatataatt tatcaaaaat
     1921 accacatccc caacattaat ctcgccatca tagttggcat cgccggttaa ataaggatgc
     1981 tgatggaccg cgaggtcaaa cgcctgtatg gtttctttcg gcggttctga tgagtcggta
     2041 accctgaggc tgaatccata tgtcgtggcg taagtcggaa tccctgacaa ataggcgctg
     2101 tcgttatagg tgaatgataa gccgtagggc agctgtccgc cgacatgttc ccatatgtac
     2161 ggcggtgtgc catttatcgc ggtaaatttc tggtagtaaa atgagtcaac atagccgtcg
     2221 gcaagatcca acgtctgaac cagcaattct cccgcaaaag cacgcagaaa gcggggccag
     2281 ttagtctggt tgaagtcgat cttcgattga tagtcgtcaa cgtcgggatg tcccgagtag
     2341 tcattgggaa accataacg
//
LOCUS       DSEB01000434            5810 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000947, whole genome shotgun sequence.
ACCESSION   DSEB01000434 DSEB01000000
VERSION     DSEB01000434.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5810)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000947"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1335
                     /locus_tag="ENO07_07800"
     CDS             <1..1335
                     /locus_tag="ENO07_07800"
                     /inference="COORDINATES: protein motif:HMM:PF14684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01911.1"
                     /translation="EEDDPDLTQLTHSGQPKSDLKLSADSKYVFFLEGGKLKKLNIKD
                     KKVENLGFTAEMDVVVDRENRQKFQETWWMFDEYYYDPDLHGVDWDRIREKYSPLVDE
                     VETEDEFNQIILEMMGDLGSSHINIYSRGGGIKKELSTAWYGFHLDQDELDENGLYRI
                     SLVLPNSPAALADTPLDTGDYLLAINGQEINRETNIYPLFSGQIDKKTIFTVAKGPDA
                     EPYDVTVKGTEFGPIYDLFYDFWVEERRRMVDSLSDGRLAYIHIRRMYPDALEKFKLE
                     IVNQTQDKEGVVLDVRYNGGGNIAVHLLGILIKEPYIYRNFAGYPVTSENKMRSKALE
                     KPSILLINNGSGSNSEIFAEGYRKLGLGKIVGERTAGGVIGTASYNLIDGTRIRRPSW
                     GCYTTDMENLELVPRYPDITVENMLTDIVKGRDPQLERAVRELLNQIGDKTE"
     gene            complement(1350..1868)
                     /locus_tag="ENO07_07805"
     CDS             complement(1350..1868)
                     /locus_tag="ENO07_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01912.1"
                     /translation="MNKSDEAIATTIKKYSSDLPWGIYLYDISENELSHWGGQPQAGE
                     YHQTEKYLADMIKDICFMAVEKQKVYTETSPSGLVYISVPLFIDKKIWAVLATYLSLG
                     KEGTWRRTLNRGPSVTRTVDLLEELGFILSTHQEVQKWYRPSFQDESEKSPSPENEDR
                     LVSPKLSSFARW"
     gene            complement(2010..2780)
                     /gene="bshB1"
                     /locus_tag="ENO07_07810"
     CDS             complement(2010..2780)
                     /gene="bshB1"
                     /locus_tag="ENO07_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HDS01913.1"
                     /translation="MTEKYDIISIGAHPDDVEVGTGGVLIKMAQMGYRTGIVYLTKGE
                     MGTGGTPEVRSQESRKAAEIMQADLLETLDWGDTKLKDRPEYRYELAAIFRKYRPEIV
                     LAPWMSGGHGKRQSHPDHRAAGKIVTNSIYYATFKKLPIQGEIHKVKALFHYFLPVEA
                     TPTFIVDITDQYELWMEALKAHASQFMNPEKSRDYLWSLETMARSFGAMAGVKYGQAF
                     KIGEPLRIQDIFCLTRGKDETPCAFGKHPQNIKRDIQK"
     gene            complement(2835..4016)
                     /locus_tag="ENO07_07815"
     CDS             complement(2835..4016)
                     /locus_tag="ENO07_07815"
                     /inference="COORDINATES: protein motif:HMM:PF00756.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01914.1"
                     /translation="MKRSKILFTVLFLVGLILIGCSERENPADFPDINERGFKNDGVF
                     TYPRAYSAISDHAGWQFFNFTVDKNFFTKLYAPPSFTPQGRPFPILYLLHDFEGDENY
                     YFSRMVQWIADSLIYEGEIKEFYIASINASTPYGGTWYTDNEFFGWFETMVVDQWVAF
                     IESTLEDQLRVISERESRAIGGFGMGGYGALKLAAKYPELYSSVSASTPAGSFQQIED
                     LIPMMIEEQGLSIPIDPDDYQDMDTTNLFTEKPYTMLFFSLAQAFSPHPEDGEPGYAD
                     STTFLSFYGIDLPFDYNGNIYQPVFDKWLAHDLSAVFDTYGGNFDSIAVHLEVSDTNQ
                     FLFHQQAEDIRNSMDQNGINYSFDTYSGYTGYPAFNQRFVYDRLVEILKFHSENLVEE
                     M"
     gene            complement(4030..5502)
                     /locus_tag="ENO07_07820"
     CDS             complement(4030..5502)
                     /locus_tag="ENO07_07820"
                     /inference="COORDINATES: protein motif:HMM:PF03349.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01915.1"
                     /translation="MRIFNTIMLILLILLFSGEVMAGGYNVSGVGLKALSMGGAFRAV
                     ADDWSALYYNPAGLSNLYQHEIAFGIDLYSYRPEFTPQMTLNDYSFGYYDGEERVSDD
                     KIPYTPFGAAAFVLPMFEKLTVGFGVFQPYDHDVEWNVFKLSPAYNQYVDDSFDLPVV
                     EPLVPFPEITHAFNVDLIDFHPTIAAELIEDRMSLGVGLSIRKGSYNRSEIILRPNTL
                     SESFSSRPYENLVTMTEYDASGWGLGVNVGLLFQASEKLKLGASYQSKTTIDLSGDLY
                     ATLYSPGNRHLEEFADTANPANQLFKGEVYTANHEADAEWVIPSEFGFGLAYQLSERV
                     MIAADFAYIMWSENEGIEFDVTSTTGLTEFSFINGYLTPVDFIEEWDDALRISVGVEG
                     HPHEKYKLRGGYSFEQTPIPEEGARVIFDNPADRHHLSGGASVYVGIVELYGAVSMII
                     TPETTVSDLVDADEDGIFDNHTGTYSDFTAVTSIGFTVRF"
BASE COUNT         1520 a         1408 c         1446 g         1436 t
ORIGIN      
        1 gaggaagatg atcctgacct gacgcagttg acccattccg gtcagccaaa aagcgatctg
       61 aaattatctg ccgattcaaa atatgttttt ttcctggaag gcggcaaact aaagaagctg
      121 aatataaagg acaagaaagt tgagaatctt ggcttcacgg ccgagatgga tgttgtggtg
      181 gacagggaaa accgccagaa atttcaggag acctggtgga tgtttgatga atactattat
      241 gatcccgatc ttcatggagt tgactgggac aggattcggg aaaaatacag tccgctggtc
      301 gacgaggtcg agacagaaga cgagtttaac cagatcatcc tggagatgat gggcgacctg
      361 ggatcatctc acatcaacat ttattcccgc ggcggtggaa tcaaaaaaga actctcgacc
      421 gcctggtatg ggtttcatct tgaccaagat gagctggatg aaaatggcct ttacaggata
      481 agccttgtgc tgccgaactc gccggctgca ttggccgata caccgcttga tacaggcgac
      541 tatctattgg cgatcaacgg gcaggagatc aaccgtgaga ccaatatcta tccgcttttt
      601 agcggccaga tcgataagaa gactatcttt acggtggcca aaggccctga tgctgagccc
      661 tatgatgtga ctgtgaaagg gacggaattc ggccccatct acgacttgtt ctatgatttc
      721 tgggtggaag aacgccgccg gatggttgac tcactttctg acggcaggct ggcatacatt
      781 catatccgcc gcatgtatcc cgacgccctg gagaaattca aactggagat agtcaatcag
      841 acgcaggata aagagggcgt cgtgctggac gtgcgctata acggcggcgg caatattgcg
      901 gtgcatctcc tgggcatcct gataaaagag ccgtatatct atcgcaactt cgccggttac
      961 ccggtcacct cggaaaataa aatgcgcagc aaggcgctcg aaaaacccag tattctcttg
     1021 atcaataacg gttcgggttc caactccgag atttttgccg aaggctatcg caagctcggc
     1081 ctgggtaaga tagtgggcga aaggaccgcg ggcggcgtga tcggaacggc ttcttataat
     1141 ctgatcgacg gcacgcgtat caggagaccc tcatggggat gctacacgac cgatatggaa
     1201 aatttagaat tggtgccgcg ctatcctgac attacagttg agaacatgct tacagatatt
     1261 gtcaaaggaa gggatccgca actggagcgt gcagtaagag agctgctcaa tcagattgga
     1321 gataaaaccg aataggagac tccaaaattc taccatcttg caaacgagga taacttcggg
     1381 ctcaccagcc tatcctcgtt ttcaggggag ggtgattttt cggattcgtc ctggaaactg
     1441 ggacggtacc atttctgtac ctcctggtgc gtggagagaa taaatccaag ctcctccagg
     1501 agatcgaccg tcctggttac cgagggtccg cggttcaatg tacgccgcca ggtaccctct
     1561 ttaccgagcg agagataggt tgccagcaca gcccagattt ttttatcgat gaaaagggga
     1621 accgatatat ataccagtcc ggagggggat gtctcagtgt agaccttttg cttctcaacc
     1681 gccataaaac agatatcttt gatcatatcg gccaggtatt tttcggtctg atgatactcc
     1741 ccggcctgcg gctggccgcc ccagtggctc agttcgtttt cagaaatatc atagagataa
     1801 ataccccagg gaagatcaga ggagtacttt ttaatagtcg ttgcaatcgc ctcgtcggac
     1861 ttattcatct gaaacaaatc tcccttgtac aggcggagat ttctccgctt ccattcattt
     1921 ctaataaata tgtcgaccgg aatcagcgga atataacatg attaggaatt attgctcaca
     1981 gaacggtcag cgggaatgga tgtgctgttc tatttttgaa tatcccgctt tatattttgg
     2041 ggatgtttgc cgaatgcaca gggcgtttca tctttgcccc gggtgaggca gaaaatatcc
     2101 tggattctga gcggctcacc tattttaaag gcctgcccgt acttaacccc ggccatggcg
     2161 ccgaacgagc gcgccatcgt ctccaaagac cataaataat ccctggattt ctcgggattc
     2221 atgaactgcg aggcatgagc cttgagcgcc tccatccaga gctcatattg atcggtgata
     2281 tccacgataa atgtcggcgt ggcctcgacc ggtaggaaat agtgaaagag agctttgact
     2341 ttgtgaattt caccctggat ggggagcttc ttgaaggtgg cgtaatagat ggagtttgtc
     2401 actattttgc ctgctgcccg atgatccgga tgtgactggc gtttgccatg cccgccgctc
     2461 atccaagggg ccagcactat ctccggcctg tattttctaa aaattgcagc cagctcgtag
     2521 cggtactcgg gacgatcctt gagtttggta tcgccccagt ctagggtttc caggagatcg
     2581 gcctgcatga tttcggccgc cttcctggat tcctgcgatc tgacctccgg ggtacccccc
     2641 gtgcccatct cccctttggt aagatagacg ataccggtac gatagcccat ctgagccatc
     2701 tttatcagta ctccaccggt tcccacctcg acatcatcgg ggtgcgcccc gattgaaatt
     2761 atgtcgtatt tttcggtcat aacaaaaaaa gatgctgccc gttatgcggg cagcatctat
     2821 aatagtacct tagtttacat ttcctctaca agattctctg aatggaactt gagtatttcg
     2881 accagccggt catatacgaa ccgctgatta aacgccgggt agcccgtgta gccggaataa
     2941 gtgtcgaagg aatagttgat tccattctga tccatggaat tgcggatgtc ctcagcctgc
     3001 tgatggaaga ggaactgatt tgtatcgctc acttcgaggt gtacggcgat agaatcaaaa
     3061 ttcccgccat acgtatcaaa aacagcgctc aaatcatgcg ccagccattt atcgaaaacg
     3121 ggctgataga tattgccgtt atagtcgaag ggcaggtcga taccgtagaa gctaaggaat
     3181 gttgtcgaat cggcgtagcc gggctctcca tcttccggat gcgggctgaa tgcctgagcc
     3241 agagagaaga aaagcatggt gtacggcttc tcggtaaaca ggttggttgt atccatatcc
     3301 tggtaatcat cgggatcgat ggggatgctg aggccctgct cctcgatcat catcgggatc
     3361 aaatcctcga tctgctggaa gcttccggca ggcgtcgagg ccgaaaccga agaatacagt
     3421 tcaggatatt tggccgccag cttgagagcg ccatacccgc ccatgccgaa accgccgatg
     3481 gcccgggatt ctcgttcact gataacccga agctgatcct ccagggtact ttctataaag
     3541 gcaacccact ggtcaacaac catcgtttca aaccacccaa aaaactcgtt atcggtatac
     3601 caggtgccgc cgtaaggggt ggaggcgttg atgctggcaa tgtagaactc cttgatctcc
     3661 ccctcgtaaa tgagactgtc cgcgatccac tgaaccatcc tggagaaata gtagttttca
     3721 tcaccttcaa aatcgtggag aaggtaaagg atcgggaaag gacgtccctg gggagtaaat
     3781 gacggtgggg cgtagagctt ggtaaagaaa ttcttgtcca ccgtaaaatt aaagaattgc
     3841 cagccggcat gatcggaaat cgctgaatag gcgcgggggt atgtgaacac accgtcattt
     3901 ttaaatccgc gctcattgat atccggaaaa tcggctggat tctcgcgctc cgaacagcca
     3961 atcagaatca atccgaccag aaacaaaacc gtaaataata tctttgatct cttcattgtt
     4021 cacaacctcc tagaacctga cggtgaatcc gattgaggtg acagccgtaa agtcactgta
     4081 tgtgccagta tgattatcaa aaattccatc ttcatcggca tcgaccagat ccgagacggt
     4141 ggtttcaggt gtgataatca ttgatacggc gccatagagt tcaactattc cgacataaac
     4201 tgaggcgccg ccgctcaggt gatggcggtc ggcgggatta tcgaaaatta ctcgtgcacc
     4261 ctcctcaggt ataggagtct gttcgaagct gtaaccgccc ctcaatttgt atttttcgtg
     4321 cggatggccc tcgacaccga cggaaatcct gagagcatca tcccactcct cgatgaagtc
     4381 gacgggagtc aggtaaccgt ttatgaatga gaactcggtc aaaccggtgg tgctggtaac
     4441 atcaaattca atgccctcgt tctccgacca cataatatag gcaaagtctg ccgcgatcat
     4501 aaccctctcg ctgagctggt aggccaagcc gaatccaaat tcggagggga ttacccactc
     4561 ggcatcggct tcgtgatttg cggtgtaaac ctcgccctta aatagctggt tggcgggatt
     4621 ggcagtgtcg gcaaattctt ccaggtgccg gttgccgggt gaataaaggg tggcataaag
     4681 gtcacccgaa agatctattg tcgtcttgga ctgataagag gcgcccagtt tcaacttttc
     4741 actggcctgg aagagcagcc cgacattcac tcccagaccc catccgctgg catcatattc
     4801 agtcatggtg accaggtttt catagggacg ggaggagaac gattccgaaa gagtgttcgg
     4861 gcgcagtatt atttcgctgc ggttgtacga acccttacgg attgaaagac ccacgcccag
     4921 ggacatcctg tcttcgatca actcagccgc aatggtggga tgaaaatcga taaggtcaac
     4981 attgaaagca tgagtaattt ccgggaaggg aaccagcggc tcgaccaccg gcaaatcgaa
     5041 tgaatcgtct acatactgat tgtaggccgg gctgagcttg aatacattcc attccacatc
     5101 atgatcgtag ggctggaaca ctccgaatcc cacagttaat ttttcaaaca tcggaagtac
     5161 aaatgcggcg gctccaaagg gagtgtaggg aatcttgtca tcagaaaccc tttcctcgcc
     5221 gtcatagtat ccaaatgaat agtcgtttaa tgtcatctgc ggagtaaact ccggtcgata
     5281 gctatagaga tcgattccaa atgctatctc atgctggtag aggttcgaca gccctgcagg
     5341 attgtaatac aatgcgctcc agtcatcggc caccgctctg aacgctccgc ccatagagag
     5401 cgctttcagg ccgactccgg agacattata accgcctgcc atcacctcac cagaaaataa
     5461 cagaatcagg agtatcagca tgattgtgtt aaaaattcgc atggttttta ctatctccag
     5521 ttaaatagat taatttccca ataaatagca tcccaaggct ttaagtcgtt gccaaacaaa
     5581 ttctttctct gcaaaaaagt aaaatccaaa actatattat aatcccgcct ttgtcaacac
     5641 ttttttggtc ttttgatact gtcataattc ttgccatcag atgaagagac gtttatattt
     5701 caagaatgtc caaaacccga tgttcacttt ttctgcttga tcgccaaaat gataggagcg
     5761 cgaaacttta gaccggaaat ggtgtttaat ctcacctgga agattataga
//
LOCUS       DSEB01000435            5835 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000940, whole genome shotgun sequence.
ACCESSION   DSEB01000435 DSEB01000000
VERSION     DSEB01000435.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5835)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000940"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(104..1354)
                     /locus_tag="ENO07_07825"
     CDS             complement(104..1354)
                     /locus_tag="ENO07_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002352705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDS01916.1"
                     /translation="MSYLKETDPEIYEAIMKETERQNEKIELIASENFVSRAILEAAG
                     GVMTNKYAEGYPGKRYYGGCEYVDIAEDLARERAKELFGAEHANVQPHSGSQANMAVY
                     FAFLKPGDTVLGMDLSCGGHLTHGHPINFSGIFYNVIGYTVDKETEQIDYDELAKLAE
                     KHKPKMIIAGASAYPRIIDFEKFRKISDSVGALLMVDMAHIAGLIVAGLHPSPVPHAD
                     FVTTTTHKTLRGPRGGMILCKEKYAADLDRMVMPGIQGGPLMHIIAAKAVALKEAARP
                     EFKEYQAQIVKNAKAMADRFLEHGYNLVSGGTDNHLMLMSFIGMDMTGKQVEKSLDKC
                     SITVNKNTVPFDPEKPFVTSGIRIGTPAVTSRGMKEPEMVRIADLIDRVIQNRKNKEV
                     YAEVKEGVAKLCSEFPLYQEILED"
     gene            1507..1692
                     /locus_tag="ENO07_07830"
     CDS             1507..1692
                     /locus_tag="ENO07_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01917.1"
                     /translation="MSPGIAAGQIRAKSNEHKPIGDGKENWPDLIAALRQHSPGAIIV
                     AESDSLKNNKISLERLK"
     gene            complement(1866..4058)
                     /locus_tag="ENO07_07835"
     CDS             complement(1866..4058)
                     /locus_tag="ENO07_07835"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00246.22,HMM:PF05547.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01918.1"
                     /translation="MKKAPLVILILSILLTTSIISAASTTMQVRIYIDSKAQLSELRS
                     LHLDIVYRQDNYVEIITDAEELEELQALDFRTEVIHEDLVAFYQSRLAPKDMGGYMTL
                     SEINAKTDSLVDNFPDIVSQKYNLGQTIEGRDMWAIKISDNPDVDEDEPEVFYTAAIH
                     AREVITPLVLFNFADSLTQKYSTDTQIQNLVDNREIWFCFCVNPDGYYYNEYTDPGGG
                     GMWRKNRRHNFDGSYGVDLNRNFGYEWGYDDEGSSPVPSDATYRGTMGFSEPETQNMR
                     DFHYEREFILSVYFHSYSDLILWPWGYDQFYTEDQDIFQVMGDSIATWNGYAPSPAWG
                     LYVANGTTDDWIYGEQTYKNKTFAFTFEVGGYWDGFWPSVLDIPELVNENYMPLMFLT
                     EVAGSVYQLRAPVAPQIFAPDSIDEGEDIIVFWTFEDTLNPAVEFELVELTGQQEITD
                     YAENFDYCQNNDFILSSARSYSGLYSFFSGAENNIYRYVEYEFPFPVEAGDSLKFYTW
                     YDTELDWDYGYVEVAAGSGPFTAIEGNITTTYDPHGNNRGHGITGSSYGWVLGKFSLE
                     DFVGQNIRVRLSYETDAYTTDEGIYFDDIYPITTFENESFVTLPSDDASYMFPDKIPG
                     MYHYKIRAKDAEDQWGAYSPIDGTQVYALPTYICGDANADETVNVSDAVAIINYVFVG
                     AAAPDPMESADTNCDAAVNVSDAVMIINYVFIGGNDPCDPDGDSIPDC"
     gene            complement(4158..>5835)
                     /locus_tag="ENO07_07840"
     CDS             complement(4158..>5835)
                     /locus_tag="ENO07_07840"
                     /inference="COORDINATES: protein
                     motif:HMM:PF02518.24,HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HDS01919.1"
                     /translation="DIELKRSTINYRDIMASTVVARAEYLRETGLKTAEHETSQSYDD
                     MDLIETYYAALTERERRDLRKGEIIRCGFGQGEDNDKIAILYPFKDSDGARWIGLFFK
                     NASGLKLVNTVMKYNYLFQLAGLLAALLVAYAYLKLTLNPFKRIADEARRIKDENSAS
                     GESVEEIVETFKATIASLEEKEERLQELYSNSQERADRLEKFNQYILESMLSGLIGID
                     NSGRIVHLNRSARRILGLEENEIDYGSFRKTFAEMKTLVEVLERVLENKMSEERLEQK
                     YIRSDGKEKILGISGSPVYDHNGRLVGAILLLADLTEVRRLQSEIAFKEKMAAVGEMS
                     AGLAHEMRNAMMAIVGFSKILKKSPGDKKQIREIAGSIAAESENCENMLKRFLSFAKP
                     ADLTPETLSLPELGHSVINKLEPLADKKQVSIIDDFSQDLPDLNCDRTAIDQVITNLL
                     KNAIEASPQKGLVYVRIKHDSTLNSFIIEVEDSGPGIPEENRMKVFSPFFTTKEDGTG
                     LGLAIVKKLVSSMAGSVEIESQSGKGCKFRITITENHLQPRNSAISYQAT"
BASE COUNT         1444 a         1472 c         1330 g         1589 t
ORIGIN      
        1 atctatgacc ttggctattg cgccagccag actttcttcg tcagccggat aagttgaaaa
       61 ctccaataac atacaagtcc tccttttttt attccaataa atttcaatcc tccagaattt
      121 cctggtacag cggaaattca gaacacagtt tggcaacccc ctcttttact tctgcataga
      181 cctctttgtt tttgcggttt tgaattaccc tgtcgatcag gtctgcaatt ctgaccatct
      241 ccggttcttt cataccccgc gaggttacgg ccggtgtgcc gatccttatc ccggaagtca
      301 cgaacggttt ttcgggatcg aagggcaccg tgtttttatt tactgtaatc gaacatttat
      361 cgagcgattt ttcgacctgt tttccggtca tgtccatccc gatgaatgac atcaacatca
      421 gatgattatc tgtgccgccg gaaaccagat tgtagccatg ttccaggaac cggtctgcca
      481 tagccttggc attttttact atctgcgcct ggtattcttt gaactccggc ctggcggctt
      541 ctttcagggc cacagccttg gccgcaatta tgtgcatcag ggggccgccc tggatccctg
      601 gcattaccat tcggtccagg tcggcggcat acttttcttt gcagaggatc attccgcccc
      661 gcgggccacg taacgtctta tgggtggttg tggttacgaa atctgcatgc ggtaccgggg
      721 agggatgcag tcccgccaca atcagtccgg caatatgggc catgtccacc atcagcagcg
      781 ctcccacaga atccgatatt ttgcggaatt tttcaaaatc tattatgcgg ggataggcgg
      841 aagctccggc gattatcatc ttcggtttat gcttttccgc aagtttcgcc agttcatcat
      901 agtcgatctg ctcggtctcc ttgtccacag tatatccgat gacattatag aaaattcctg
      961 agaaattgat tggatggcca tgggtcaggt gtcccccgca ggagagatcc ataccaagaa
     1021 cagtgtctcc cggcttgagg aatgcgaaat aaaccgccat attagcctgt gaacctgaat
     1081 ggggctggac attggcatgt tccgctccga acagctcttt ggcgcgttca cgggccagat
     1141 cctcggcaat atcgacatat tcacagccgc catagtagcg cttgcccgga tagccctcgg
     1201 cgtatttgtt agtcattacg cctcccgcgg cttccagtat cgccctggag acaaaatttt
     1261 cagatgctat aagctcgatc ttctcatttt gacgctcagt ttccttcatt atagcttcat
     1321 atatttccgg gtcggtctct tttaaataac tcataccatc tccaatcctt tatccgccga
     1381 tcattttcgg cggttcactt atctataacc aagaaaaata aatgatgctc cattaaaaag
     1441 caagtctgtt ttgagattgt cttgcaagga gttaaataat cagtataaca ggctgaccat
     1501 atcgaagtgt cgcccggaat cgccgcaggc caaatcagag ctaaatcgaa tgagcataag
     1561 ccgattggcg acggtaaaga aaactggccc gatttaatcg cggcattgag acagcattcg
     1621 cccggtgcga ttatagtagc cgaaagcgac agcctgaaga acaataagat aagtcttgag
     1681 agattgaagt agatgtgatt gcggtggcac ttgttgcgtt gggtcacacc ctgactcggt
     1741 cgaagcaaga cccaacgccg ctgcatgaga aattcccgca ctgacctcaa aactaagaat
     1801 agatcaattt caaaaaagcc cgtccacaaa tgcggacggg cttgattaat gatcgatctt
     1861 cagtatcagc agtcgggaat tgaatcgccg tccggatcac agggatcgtt accaccgatg
     1921 aaaacatagt ttatgatcat gacggcgtcg ctgacattga cagcagcatc gcagttggta
     1981 tcggcggatt ccattggatc cggagcggcc gctccaacaa agacatagtt tataattgcc
     2041 acagcatcag agacattcac agtctcatcg gcattagcat caccgcagat atatgtcggc
     2101 aatgcataga cctgggtacc atcgatcggg ctgtatgcac cccattgatc ttcagcatct
     2161 ttagccctga tcttgtagtg atacatcccc ggaatcttat ccggaaacat gtatgatgca
     2221 tcatctgacg gcagcgttac aaaagactcg ttttcaaatg tggttatggg ataaatatca
     2281 tcaaagtata taccttcatc ggtcgtgtac gcatcggttt cataagaaag gcgcaccctt
     2341 atattctgcc ccacaaaatc ctccaggctg aacttaccca gaacccatcc ataagagctg
     2401 ccggtgatac catgcccgcg gttgttgcca tggggatcat aggtggtggt tatatttcct
     2461 tcaatcgctg tgaaggggcc gctgcctgcc gcgacctcga cataaccata atcccagtcg
     2521 agctcggtat cataccaggt gtagaatttg agtgaatctc ccgcttcaac cgggaaggga
     2581 aattcatatt cgacatagcg atagatgtta ttttcggcgc ctgagaagaa gctataaaga
     2641 ccggaataag atcgcgccga ggatagaata aaatcgttat tctggcagta gtcgaaattt
     2701 tcggcataat cggtgatctc ctgctgtccg gttaactcta ccagttcgaa ttccacggcg
     2761 ggattcaggg tatcctcaaa ggtccagaag actattatat cttccccctc gtcaatgctg
     2821 tcaggagcaa aaatctgagg agcaacaggc gcacggagct gatatacaga gcctgccacc
     2881 tcagtcagaa acatgagagg catgtagttt tcattcacca gttctggaat atctaatacc
     2941 gatggccaga aaccgtccca gtagcctcca acctcgaatg tgaaagcgaa agtcttattt
     3001 ttataggtct gctcaccgta aatccagtca tcggtagtgc catttgctac ataaagaccc
     3061 catgccggtg acggggcata tccgttccag gtcgctatac tgtctcccat aacctgaaaa
     3121 atatcctggt cctcggtata aaactgatcg tatccccagg gccagaggat caagtctgaa
     3181 taagaatgaa aataaacgga taatataaat tctcgttcat aatgaaaatc gcgcatattc
     3241 tgggtttccg gctcggaaaa gcccattgtg cccctgtatg tcgcatctga aggaaccggg
     3301 gatgatccct cgtcatcata accccattca tagccaaagt tgcggttcag atcaacacca
     3361 taggagccgt caaaattgtg cctgcggttt ttacgccaca tcccgcctcc acccggatcg
     3421 gtatattcat tatagtagta gccatccgga ttaacacaga aacagaacca gatctcgcga
     3481 ttatcaacca gattctgaat ctgggtatcg gtggaatatt tctgcgtcag agaatcggca
     3541 aaattgaaaa gcacaagcgg ggtgatgacc tcgcgtgcat ggatagcggc ggtataaaag
     3601 acctcgggtt catcctcatc aacatcggga ttatctgaaa tctttatcgc ccacatatcc
     3661 cggccctcga tagtctggcc caggttatat ttctgggata cgatatccgg gaaattatcc
     3721 accaatgaat cggttttggc gttgatctca gaaagggtca tatagccgcc catatccttg
     3781 ggggccagac gggactggta gaacgccacc agatcctcat gaatcacctc ggtcctgaaa
     3841 tcaagtgcct gcagttcttc cagctcctca gcatcggtga taatctcgac ataattgtcc
     3901 tgcctgtaaa caatgtcgag atgcagactt cgaagctctg agagctgagc tttggaatcg
     3961 atatagattc tgacctgcat tgtggttgaa gcggcagata tgatgctggt agtcagcaat
     4021 atggaaagaa tcaaaattac gagtggggcc tttttcatta taaatcctcc tatgaaggct
     4081 cgcaggatgg tagtttgaat ccagttgttt tatataatat acgatgaaga ttggattttg
     4141 caagcttaag attatactta agttgcctga taggatatgg cggaattcct gggctgaagg
     4201 tgattttcgg tgatagttat ccgaaatttg catcccttgc ctgactggct ttcaatttca
     4261 acgcttccgg ccatactgct aaccagcttc ttgactatgg caaggcccaa gccagtacca
     4321 tcctcttttg ttgtaaagaa tggcgaaaag accttcatgc ggttttcctc cggtataccc
     4381 ggcccgctat cctcgacctc aattataaat gagttcaagg tggaatcatg tttgattctg
     4441 acatatacca ggcctttttg aggtgaggct tcgatggcgt ttttcaaaag gtttgttatg
     4501 acctgatcga tcgcggttct atcgcagttc aggtcgggga gatcctggga aaaatcatct
     4561 attattgaaa cctgcttttt atcggccaac ggctccagct tgtttattac ggagtgtccc
     4621 agctcgggca atgagagcgt ctccggtgtc aggtcggccg gcttggcaaa agacagaaac
     4681 cttttcagca tgttctcgca attctctgat tcggcggcaa tggagccggc tatctcccgg
     4741 atctgttttt tatcgccagg actttttttc aggattttgc tgaagcccac gatagccatc
     4801 atagcattgc gcatttcatg tgccagtcca gcgctcatct cccccaccgc ggccattttt
     4861 tccttgaagg cgatctctga ctgcagcctg cgcacctcgg taaggtcggc cagcaataaa
     4921 atcgccccca ccagccttcc attatgatca taaaccgggg aaccggagat accaagaatc
     4981 ttctccttgc cgtccgatct gatatatttc tgctccagac gttcctcgga cattttattt
     5041 tccaggaccc tctcgagaac ctccaccagc gttttcattt ctgcaaatgt ttttcgaaag
     5101 gagccatagt cgatttcatt ctcctccaat ccaagtatcc tgcgggcgga gcggttcaaa
     5161 tgtacaatgc gtccgctgtt atctatgccg atcaggccgg agagcatact ttcaagaatg
     5221 tattggttga atttttccag gcggtcggca cgttcctggg agttgctgta aagctcctgc
     5281 agtctctcct ctttttcctc caggctggca atcgtggcct taaaagtctc cacaatctct
     5341 tccaccgatt cgccgctggc actgttctca tctttgatcc tgcgggcctc atccgcaatt
     5401 cttttaaatg ggttcaatgt cagtttcagg taagcatagg cgaccagcag cgctgccaga
     5461 aggccggcca gctggaaaag gtaattatat ttcatgactg tgtttacgag ttttaatccg
     5521 gaggcattct tgaaaaacaa cccgatccag cgcgcgccat cactatcttt gaatgggtag
     5581 agtatcgcga ttttatcatt gtcctcaccc tgtccgaatc cacacctgat gatctcgccc
     5641 tttcgcaggt cacgacgttc cctttcggtc agggcggcat aatatgtttc tatcagatcc
     5701 atatcatcat atgattggga ggtctcatgc tcagcggtct tgaggccggt ttccctcagg
     5761 tattccgctc ttgccacgac ggtcgaggcc attatatccc ggtaatttat ggtcgagcgc
     5821 ttgagctcta tatcg
//
LOCUS       DSEB01000436            1615 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1016243, whole genome shotgun sequence.
ACCESSION   DSEB01000436 DSEB01000000
VERSION     DSEB01000436.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1016243"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..986)
                     /locus_tag="ENO07_07845"
     CDS             complement(<1..986)
                     /locus_tag="ENO07_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDS01920.1"
                     /translation="MSAKFNIGERSGYAGVPLVFRLFLFFGFIVIALVFVYYTQNVVG
                     QLKSNSLRMVNLSIKLLQVELSQETSGEATGVIFDEIIAPTEIPIIITDRNNEPLFFR
                     NIQGLDTNAVTDENREKLMERIELMNEKYGRFPIKHIDQATGTETIINYFYYGDPQLI
                     RQLQAMPFIEIGIVAAFLLLGYIGYRNIKRSEQHFIWVGMAKETAHQLGTPISSLMGW
                     LEILNIWYDEEKKAGVDEEELSQIEDVSQQMTRDIERLQKIANRFSQIGSRPALKSAD
                     LNKVIEDVVEYFRERLPYQGKGITISFMPGQIESVRINSELIGWVIENLVKNS"
     gene            complement(986..>1615)
                     /locus_tag="ENO07_07850"
     CDS             complement(986..>1615)
                     /locus_tag="ENO07_07850"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDS01921.1"
                     /translation="EDMYELENSEMFLIPTAEVPVTNIHADEILSEDDLPIYYVAYTP
                     CFRREAGAHGKDTRGLIRVHQFDKVELVKVVKQENSRDELETLLSQAEKVLRLLNLPY
                     RVRILCTADLSFAAAKCYDIEVYSAGVDKFLEVSSCSIYNDFQARRMNLRYRPGDGKK
                     PLFCHTLNGSGLALPRTVIAILENYQTERGTVVVPEVLRPYMGGIEEID"
BASE COUNT          413 a          425 c          353 g          424 t
ORIGIN      
        1 gaatttttta ccagattttc aattacccag ccaatcaatt cagaattgat cctgacactt
       61 tcgatctgac cgggcataaa actaatcgta atacctttgc cctggtatgg cagacgttcg
      121 cgaaaatact cgaccacatc ttcaatgacc ttattcagat ccgcagactt aagcgccgga
      181 cgcgagccga tttgtgagaa gcgattggca atcttctgca gccgttcaat atcgcgtgtc
      241 atctgctggg agacatcctc gatctggctc aactcctcct cgtccacacc ggccttcttc
      301 tcctcatcat accagatatt caagatttcc agccagccca tcaaagatga tatcggcgta
      361 cccagctgat gagcggtttc cttggccata ccgacccaga taaaatgctg ttcggatcgt
      421 ttgatattac ggtatccgat atagcccagc agcaggaagg ccgccactat cccgatttct
      481 atgaaaggca tggcttgaag ctgccggata agctgagggt caccatagta gaagtaattt
      541 attatggtct ccgtccccgt tgcctggtct atatgcttaa tcggaaagcg gccgtatttt
      601 tcgttcatca attcaatccg ttccatgagc ttttcacggt tttcatcagt gaccgcattc
      661 gtgtccagac cctggatatt tctaaaaaag agaggttcgt tgtttctatc agttataatt
      721 atggggatct ctgtgggagc gataatttcg tcaaatatta caccggtcgc ctcgcccgaa
      781 gtttcctggg aaagctcgac ctgcaggagc ttgatgctca ggttaaccat gcgcagggaa
      841 ttggatttca gttgcccgac gacattctgt gtatagtaaa caaataccag cgcaatgact
      901 ataaatccga aaaacaggaa cagcctgaag accaggggca cgcctgcata accggatctt
      961 tcgcctatat tgaatttagc agacatcagt caatttcttc tatgcctccc atatacggac
     1021 gcaacacctc cggaacaacc accgtgcccc tctcggtctg gtaattttcc aggattgcaa
     1081 ttactgtccg cggcaaggcc agtccggagc cgttcagggt atgacagaag agcggtttct
     1141 tgccgtctcc gggacgatac ctgaggttca tgcggcgggc ctggaaatca ttgtagatcg
     1201 aacaggagga gacctccaga aatttatcaa cacccgctga ataaacttct atatcataac
     1261 atttggctgc cgcgaatgac aaatcggcag tgcatagaat tctcacccgg taaggaagat
     1321 tcaaaagccg caacactttc tcggcctggc tcagaagcgt ctccagctca tccctggagt
     1381 tttcctgttt gaccactttg acaagctcga ccttgtcgaa ttgatgtact ctgatcaggc
     1441 cgcgggtatc cttgccatgt gctcccgcct ctcggcgaaa acagggcgta taagcaacat
     1501 aatatatggg taaatcatct tcgctcagaa tctcatccgc atgaatattg gtgacaggca
     1561 cctccgcagt cggtatcaag aacatctcac tattctcgag ttcatacata tcctc
//
LOCUS       DSEB01000437            2929 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004330, whole genome shotgun sequence.
ACCESSION   DSEB01000437 DSEB01000000
VERSION     DSEB01000437.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004330"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..526)
                     /locus_tag="ENO07_07855"
     CDS             complement(<1..526)
                     /locus_tag="ENO07_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01922.1"
                     /translation="MRFRNKYIIPILIFAVLLILFFNLGQWYIFSQVRDFAESVFREN
                     LLAQAELAASSFNGDAILSLQGDDFYAPDLIDIKDRLDRLKDDYDFFNVRLLDLDAER
                     LLGFADYDTASFIFDYDLGPFISASAGIPAASDLITARDMYLISAYAPITTSLDSVVA
                     VLGIDADYTFFKSLA"
     gene            798..1880
                     /locus_tag="ENO07_07860"
     CDS             798..1880
                     /locus_tag="ENO07_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01923.1"
                     /translation="MKNAIIITLALTLAGLFILTGCSDNSASSDAEGSLLNMDDFFGG
                     YSPTDEAPAFGDEEISRNFPEPEVVSSELNSFDDLTSIQGEPDVSVYSMRIMWGMLEY
                     DSTVTSLTDWSGSLTVDTGCIKVINKILFEPGQDYFIRPRTDCQTLEWVSFTNVHYDG
                     ILVFMYFRNADFDPATTNVVFETGPYSRTFALAELDSLAEIVDVDDLGNKVSIEAAKL
                     ERLECPEGYLEGRWVRSGNQRIFGQFYGRFISLDGLFMGHVKGHWGVNSEEEKLFFGK
                     WIDHIGRFQGFLRGHWGFDDTAREAESSGWYEGNYFSASLTNLGTLNGNWVSHHRGSM
                     PNSGATDKVPKHARGFFHGTWKQYCN"
     gene            complement(2007..2699)
                     /locus_tag="ENO07_07865"
     CDS             complement(2007..2699)
                     /locus_tag="ENO07_07865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HDS01924.1"
                     /translation="MVKENLLNAEVRQDAGKGAARKLRAKGIIPAVMYGEDTDPVPLS
                     ISDYDFSKLISKAARSSMIIDLTVGDSEPQKVIIKEVQTDPVSSKINCVDFKKISMDK
                     KITLSLPIKLVGTPVGVKDQGGILQTIRREIEISCLPGNIPEKVELDVSGLSIGDSLH
                     YEDIDMEGVEVISDLELSVATVVAPTVMKEKVEAVEGEELEEGVEAEAAEGEGESAEP
                     ERIGEKGKEEEE"
     gene            complement(2714..>2929)
                     /locus_tag="ENO07_07870"
     CDS             complement(2714..>2929)
                     /locus_tag="ENO07_07870"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:PF14572.4"
                     /note="catalyzes the formation of 5-phospho-alpha-D-ribose
                     1-phosphate from D-ribose 5-phosphate and ATP; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HDS01925.1"
                     /translation="VFACATHGVFSGESLQRIINSPIKELIVTDTIDSTGKNLPDKIK
                     VLTTAEIFGEAIRRISNKESISILFDR"
BASE COUNT          770 a          700 c          684 g          775 t
ORIGIN      
        1 ccgccaggga cttgaagaat gtataatcag cgtcaatacc cagtactgcc acaaccgaat
       61 ccaggctggt agttatgggc gcgtaagcgg agatcagata catatctctg gcggttatga
      121 gatcggatgc cgctggtatc cctgccgagg ccgagataaa cggtcctaaa tcatagtcga
      181 agatgaaact ggcggtatca tagtcggcga agcccagaag cctctcggca tcgagatcga
      241 gcaggcgcac attgaagaaa tcgtaatcat ctttcagcct gtcaagcctg tcttttatat
      301 cgatcaaatc gggggcataa aaatcatcgc cctgcaggct caggatggca tcgccgttga
      361 aagatgatgc tgccagttcg gcctgggcca ggaggttctc ccgaaataca gattctgcaa
      421 aatccctgac ctgtgaaaat atataccact gccccaggtt gaagaaaagt atcagcaata
      481 ctgcaaaaat cagtatgggt atgatatatt tgttacgaaa tctcatttta ttgaataatt
      541 gtacacctga aaactgataa aaacaattta ttttgtacaa atcggtacaa tctgcacata
      601 aatttacgtt caactttgtt catttcatga ccaatccatt gccgcagaga ttgcgccgct
      661 gattaaattc gttaaaacac aagaggatac gaagactatt caaaattggc acagcatctg
      721 caataaaaag cggtggaaga taagagatat aatacgctgg cgtatgatgt gaaattaaaa
      781 gttaaggagt ttcgaaagtg aaaaatgcga ttataataac cctggcgttg actctcgctg
      841 ggctttttat actgacgggt tgttcagaca attcagcatc cagtgatgcc gaaggcagcc
      901 tcttaaatat ggacgatttc tttggcgggt atagtccaac ggatgaggcg cctgcctttg
      961 gagacgagga gataagtaga aacttcccgg aaccagaagt tgtttcctcc gaactgaaca
     1021 gttttgatga cctgacttca atacaaggcg aaccggatgt ctcggtttac tcgatgagaa
     1081 tcatgtgggg gatgttggag tacgattcca ctgtaacatc cctcactgat tggtctgggt
     1141 cgcttacggt tgacaccggc tgcatcaaag tgatcaataa aattctgttc gaacccggac
     1201 aggattattt tatacggccc cgcaccgact gccagacgct tgaatgggtt tcattcacaa
     1261 atgtgcacta tgacggtatt ctggtcttca tgtatttccg gaatgcagat tttgacccgg
     1321 ccacgacgaa tgttgtcttc gaaaccggcc catacagccg aacattcgcg ctggcagagc
     1381 tggattcgct ggctgaaatt gttgatgtcg atgatcttgg aaacaaggtc tccatcgaag
     1441 ctgccaaact cgagcgcctg gagtgcccgg agggctatct cgagggtcgc tgggtaagga
     1501 gcggaaacca gagaatattc ggccagtttt acggccgttt tatctccctt gacggcctgt
     1561 ttatggggca tgtcaagggt cattggggag taaattccga agaggaaaag ctgttcttcg
     1621 gcaaatggat cgatcatatc ggccgattcc agggattcct caggggtcat tggggatttg
     1681 acgataccgc ccgggaggcc gaatcttccg gctggtatga aggcaattat tttagcgcaa
     1741 gcctgacaaa tctgggaact ttgaacggaa attgggtatc acaccataga ggcagtatgc
     1801 ctaactctgg cgctactgat aaggtcccaa aacatgccag aggattcttc catggaacat
     1861 ggaagcaata ctgcaattaa aacctcacca atctgcttcg caggagccct ccaccctcgg
     1921 agggcttttt ttattgaagc aaatcaaaaa aacggccagg ttgattgcct ggccgtatat
     1981 agagattaat actaagctct gaattattat tcttcttctt ctttgccctt ctctccgatg
     2041 cgttcgggtt cggctgattc cccttctccc tcggctgctt cggcctcgac gccctcttcg
     2101 agttcctcgc cttcaaccgc ttcaaccttc tccttcataa ccgtaggagc gacaactgtc
     2161 gccaccgata gttcgagatc ggagatcacc tcgacaccct ccatatctat atcctcataa
     2221 tgcaggctgt cgccgatact gaggccggaa acatcaagct cgaccttctc cggaatattt
     2281 ccgggcaggc atgagatttc aatctcacgc cgaattgtct gcagaattcc gccctggtcc
     2341 tttacaccca ccggagttcc caccagcttg ataggaaggg aaagcgtaat cttcttatcc
     2401 atcgatattt tcttgaaatc gacgcagttg attttggatg aaaccgggtc ggtctgcact
     2461 tctttaataa ttaccttctg cggctctgaa tcacccacag tcaaatcgat aatcatggaa
     2521 cttcgggccg ccttcgagat caatttgctg aaatcgtaat ccgagattga cagcggcacc
     2581 ggatcagtat cctcaccgta cattaccgcg ggaataatgc ctttggcgcg cagcttccgg
     2641 gcagctcctt ttcctgcatc ctgtcggact tccgcgttta gtaaattctc tttcacaata
     2701 actccttaaa ttattatctg tcgaaaagta tagaaatcga ttccttattt gaaattcttc
     2761 tgatcgcctc tccgaatatt tcagctgtag ttagaacctt gattttatcc ggaaggttct
     2821 ttccggttga atcaattgta tctgtgacga taagttcctt gatcggcgaa ttaattatgc
     2881 gctgcaggga ctcaccggag aagaccccgt gcgtggcgca ggcaaagac
//
LOCUS       DSEB01000438            2928 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004336, whole genome shotgun sequence.
ACCESSION   DSEB01000438 DSEB01000000
VERSION     DSEB01000438.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004336"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..379
                     /locus_tag="ENO07_07875"
     CDS             <1..379
                     /locus_tag="ENO07_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDS01926.1"
                     /translation="IDTFRASGAGGQHVNKVSSAVRITHLETGIVVSCQSQRSQLKNK
                     ESAMKVLRARLYQLYKEKEDAKREKAESEKKKIEWGSQIRSYVFHPYNLVKDHRTEHE
                     TSNAEAVMEGELDEFINAYLTTK"
     gene            390..1388
                     /gene="pta"
                     /locus_tag="ENO07_07880"
     CDS             390..1388
                     /gene="pta"
                     /locus_tag="ENO07_07880"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002597490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDS01927.1"
                     /translation="MSILDKLQEKAKGLGKHIVLPEGDDRRTIEAAKTIIDEGIAKLT
                     ILGTPRIIEGHAKEVGFDLSSARIIEPTGSDDLDRYADEFVKLRAKKGVSKEQALNKM
                     KEVLYFGDMIVHMDEADGCVAGAAHTTGDVIRAGLHVIGLAKGISSVSSCFMMVVPEF
                     LGVENKVFLFADGAVLPNPTPEQLGAIAVSTARTMKVLVGEEPRVAMLSFSTKGSAEH
                     PDITKVRMGLEEAKKIDPKLIIDGELQVDAAIIPKIANSKAPGSPVEGKANVLVFPDL
                     DAGNIAYKIVQRLAKAEAIGPIIQGLAKPANDLSRGCSASDIVNVVAIASILSTIE"
     gene            1417..1707
                     /locus_tag="ENO07_07885"
     CDS             1417..1707
                     /locus_tag="ENO07_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDS01928.1"
                     /translation="MPTYQYQCTECSYEFEEFQNISDDPIQTCPECGGRVKRLISGGA
                     GLLFKGSGFYITDYRSPKYQADAKKESSDKKFEKTEKKSEKKVDKKADKKSA"
     gene            complement(1894..2307)
                     /locus_tag="ENO07_07890"
     CDS             complement(1894..2307)
                     /locus_tag="ENO07_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDS01929.1"
                     /translation="MEDGGGIAIMSFLIMLAIVVFYAAVAWKINVKAGEPGWGALIPI
                     YNIYLHLMIADKPGWWLIWYFVPFANIIVEIIVTIEIAKKFGKSTEFGVGLIFLSFIF
                     YPILAFGDAKYQGTPGRYARLYDGSRAYDTGSGGA"
     gene            complement(2418..>2928)
                     /locus_tag="ENO07_07895"
     CDS             complement(2418..>2928)
                     /locus_tag="ENO07_07895"
                     /inference="COORDINATES: protein motif:HMM:PF08459.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01930.1"
                     /translation="EYRHFKIKTVRGQDDFASLREIVFRYFRRLKTEDRDKPDLLVID
                     GGKGQLSAGLDALKALDIKDQQVIGLAKRLEEVFIPGQKESIIIPQGLALIEITSENP
                     QRGPPLCDRIQSQTSRETYHRHRTSENSRHWPKEGRGAFKAFRLCQEDQRTAVRADCR
                     GTQYRHLRR"
BASE COUNT          790 a          695 c          757 g          686 t
ORIGIN      
        1 aatagataca ttccgcgcct ccggggcggg cggacagcat gtcaacaaag tctcctcggc
       61 cgtgcgtata actcatctcg aaaccgggat tgtggtcagc tgccagtcgc agcggtcgca
      121 gttgaaaaat aaggaatcgg ccatgaaggt gctcagggca cgtttatacc agctctataa
      181 agagaaggag gacgccaagc gcgagaaggc cgaatcggag aagaagaaaa ttgaatgggg
      241 atcgcagata cgatcctacg tttttcatcc atataatctc gtcaaggatc atcgcacgga
      301 gcatgaaacc tccaatgccg aggcggttat ggagggcgag cttgacgaat ttattaatgc
      361 ctacttaacg acaaagtgag gtaattgaaa tgagcatcct ggataaactt caggaaaagg
      421 caaaggggct gggcaaacat atcgtcctgc ccgagggtga cgaccgccgt acaatagagg
      481 ccgccaaaac tattatagat gaaggcattg caaagttgac catcctgggt acacccagga
      541 ttattgaggg gcatgccaaa gaggttggat tcgatctctc ctcagccaga attatcgagc
      601 ccacaggttc agatgatctt gacagatatg ccgatgagtt cgtgaaactg cgcgccaaaa
      661 aaggcgtgag caaagagcag gccctgaaca aaatgaaaga ggtgctctat tttggtgata
      721 tgatcgtgca tatggacgaa gctgatggat gtgttgccgg cgcggcccat acaaccgggg
      781 atgttatccg tgccggactg catgtgatcg gattggccaa gggtatcagc tcggtctcgt
      841 cctgttttat gatggtagtg ccggagtttc tgggcgtcga aaacaaggtc tttctatttg
      901 ccgatggcgc ggtgctcccc aatccgaccc ctgagcaact gggtgcgatt gctgtttcga
      961 ctgcgcgtac tatgaaagtt ctggtgggcg aggagccgcg ggtggccatg ctgtcgttct
     1021 cgaccaaggg ttcagccgaa catccggata tcaccaaagt ccgcatgggt ctcgaggagg
     1081 ccaaaaaaat cgacccgaaa ttgatcattg acggggaact tcaggttgac gcggcgatta
     1141 tacctaagat cgccaattcc aaggctcccg gttcacctgt tgagggcaag gccaatgttc
     1201 tggtctttcc cgatctggat gccggaaata tcgcctacaa gatcgtgcag aggctggcca
     1261 aggcggaggc tatcgggccg atcatccagg gtctggccaa accggccaac gatctttcgc
     1321 gcggatgttc cgcctcggat atcgtgaatg tcgtggcgat cgcgtcgata ttgagtacta
     1381 tagaataata taaatgaaat aagcaggata tggaaaatgc cgacatacca gtatcagtgt
     1441 acagaatgca gctatgagtt cgaggagttc cagaatatct ccgacgaccc gatccagacc
     1501 tgccccgaat gcggcggcag ggtcaaaagg ttgatttcag ggggagcggg acttctcttc
     1561 aagggttccg gcttctatat caccgattat agatcgccga aatatcaggc tgacgccaag
     1621 aaagaaagct ccgacaagaa atttgaaaag acggagaaaa agtcggagaa gaaagttgac
     1681 aagaaggcag acaagaaatc agcctgaggg aagttagaat attagaataa aaatcccatc
     1741 tgcaaaagat gggatttttt atatttgcta tatcgtagcg cagggtcttg accccgacct
     1801 gtcgaaggtg tgaccctgcg cagaagacaa catgcttacc tcaaattacg aaggcctcgc
     1861 gatgtcttag caaaagctga tcccaaaaat ttttcaggct ccgccggagc cggtatcata
     1921 agcccgtgaa ccgtcgtaaa gacgggcata cctgccgggc gttccctgat acttagcatc
     1981 accaaaagcc agaatcgggt aaaagataaa gctgagaaaa ataagcccaa ccccgaactc
     2041 ggtggactta ccaaatttct tcgctatttc gattgtaaca ataatctcga ctataatatt
     2101 tgcaaagggt acgaaatacc atatcagcca ccatcctggt ttgtcggcga tcataagatg
     2161 caaatagata ttgtagattg gaatcaatgc gccccatccc ggctcacccg ctttcacatt
     2221 gatcttccat gccacggccg catagaaaac cactattgcg agcatgatta gaaagctcat
     2281 gattgcgata cctccgccat cttccatgtt tccctccaag acagatgttt tttgagtgaa
     2341 gtgtttccaa aattatagta gttcttcaaa caaagacaag ttttttctac ccgggagaaa
     2401 aataagcagc aatcttctca gcgtcggaga tgccgatatt gggtgcctcg gcaatctgct
     2461 cggaccgcag ttctttgatc ttcttgacag agccgaaatg ctttaaaagc gcctcggcct
     2521 tctttgggcc aatgccggga attttctgaa gttcggtgcc gatggtacgt ttcccgcgaa
     2581 gtttgcgatt gtattctatc gcaaagcggt gggcctcgtt gcggattttc tgaagtaatc
     2641 tcaatgaggg cgaggccttg ggggattatg atactctcct tctggccggg aataaaaact
     2701 tcctccagac gtttggccag tccgattacc tgctgatctt ttatatcgag agccttcagt
     2761 gcatccaatc ctgccgaaag ctgtccctta ccgccgtcta ttaccagaag gtcgggcttg
     2821 tcgcgatcct ctgtcttaag ccttcggaaa tatctgaaga caatctcgcg cagcgaggcg
     2881 aaatcatcct gtccgcgcac ggttttgatc ttgaaatgac gatactcc
//
LOCUS       DSEB01000439            3783 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002436, whole genome shotgun sequence.
ACCESSION   DSEB01000439 DSEB01000000
VERSION     DSEB01000439.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002436"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            317..1408
                     /locus_tag="ENO07_07900"
     CDS             317..1408
                     /locus_tag="ENO07_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01931.1"
                     /translation="MKFRTVSVILLLIAAIIMAIGCERKVVNESGGELKADASACFSC
                     HSDQERDLRAAALQWQTSKHASGDNIDRNRLNEGFYQACEQCHTSEGFLANLEGGSAS
                     GEYFTPIECFTCHAPHTTTTLEVRVQNAVALMNDFSFDRGSANLCASCHQSRANIDEL
                     ITGDTELSERWGPHHSNQSDMLAGTNAYEYGLEIDNSPHTNVAQNGCVDCHMSASGDI
                     TLGGHSWRMHDEEKDHYNVSGCNISTCHAGDIGEEFAFEADDDYDWDGNADETVQEEI
                     AGLLDSLEILLEDAGLLEEEHPTDVTVSADSAGAVFNWLFVHEDRSDGVHNTEYAAGL
                     LQAAINFMTTGDPEGVPTGNKKRSFANSR"
     gene            1462..2973
                     /locus_tag="ENO07_07905"
     CDS             1462..2973
                     /locus_tag="ENO07_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HDS01932.1"
                     /translation="MLKLRLPRLAYNFISSVGSAIAIVAGLMVVTMLVVSIFTEIENP
                     YLGIFSFMILPAILIFGLILIPVGMLRQWRREKRGERVEKTRWPYVDLNLKSHRNAFF
                     IFIFGTFFFVVFTAVGSYQAFHFSESVKFCGTTCHSVMEPEHTAYQNSPHARVACVEC
                     HVGPGAGWYTKSKLSGAYQVYATLLNKYPRPIPTPIESLRPARETCEQCHWPAKFFGG
                     MQKKIDHYMYDEESSYWPINMLIKVGGGKRGANQASGIHWHISSDVKVEYIARDHQRE
                     DIPWVRYTNLETGEVTVYHDDDDPLSQEEIDSLEPRVMDCVDCHNRPSHIYNPPDRAI
                     DDALNTRMIDPEIPEIKMVAVEAMAENYGSDEEAMEKIREHINGYYKENYSGFYEQEK
                     GLIEQAIQSTSEKFSLNIFPRMKVRWDVYPDHIGHFYFKGCMRCHEGNHSSDEGQTIS
                     HSCNSCHIILAQGSGETYEVTYQEDGLEFKHPVDIYEAWREMGCYECHTGTQP"
     gene            complement(3009..3737)
                     /locus_tag="ENO07_07910"
     CDS             complement(3009..3737)
                     /locus_tag="ENO07_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01933.1"
                     /translation="MAPYDIPTDQVEDYRKSVHGAALLEDDDLGAPACNDCHGNHGAI
                     PPDAESIDKVCGLCHNNNMVDFEKSTHADYFSVLESPACETCHSNHLIEAPGTEMLSG
                     EEPVCGQCHFEDDGTGALEAAKLMASRIDSLENAIAEAEGRLEEADQKGLFVNNGLFV
                     LKDARQRAFKARTAVHTFQADSVVNMTAAGFEQVAEARNIARELLDNYVFRRKGLAIS
                     VVVLLILAVAIWVKVKRIESRQKH"
BASE COUNT          982 a          945 c          962 g          894 t
ORIGIN      
        1 ccacagggga ctatgataac attgaagaag agttcgaatt cacggatcat accctttatg
       61 gccatatcag gaccagggag tacaggaatg tctcagccgg attcggactg acatattacc
      121 ggagcaagcg cgatctggat gtggagagct tcatcttgag gtttgatgcg gaatacgaat
      181 tctatgacgg atataaattc cgggccgagt ataacgtcca gaacttcgat gacttcctga
      241 tacgggacca gtactatacg gcaaatattg tcgaaataag tttaatcaaa catatatcct
      301 tttagggagg agatttatga aatttagaac agttagtgta atccttctcc tgattgcagc
      361 aataataatg gcgatcggct gtgagcgcaa ggtcgtgaac gaatccggcg gcgaactcaa
      421 ggccgatgcc agcgcatgtt tttcatgcca cagcgatcag gagcgcgatc tcagggcggc
      481 cgctctccag tggcagacct ccaaacatgc ttccggcgat aacattgaca ggaatcgtct
      541 caatgaaggt ttctatcagg cctgcgaaca gtgtcatacc agcgagggct tcctggccaa
      601 tttggaaggt gggtcggcat caggtgagta ctttacccct atcgaatgtt tcacctgtca
      661 tgccccgcat acaaccacca ctctcgaggt gcgggtacag aatgctgtag cattgatgaa
      721 cgatttctcg ttcgatcgcg gaagtgcaaa tctttgcgcc tcgtgccatc agagcagggc
      781 aaatatcgac gagctaatca cgggagatac cgaactcagc gaacgctggg gcccgcatca
      841 cagcaatcaa tccgatatgc tggccggcac taatgcttat gagtatggcc tggagatcga
      901 taattcacct catacaaatg tcgcccagaa tggctgtgta gactgccata tgtcggcatc
      961 cggcgatatc actctgggtg ggcattcctg gagaatgcat gatgaggaaa aggaccatta
     1021 caatgtctcc ggatgtaaca ttagtacctg ccatgccggg gacataggag aagaattcgc
     1081 gttcgaggca gacgacgatt atgactggga cggcaacgca gatgaaacgg ttcaggagga
     1141 gatagccggg cttctggata gcctcgaaat tttgcttgaa gatgccggcc tgcttgaaga
     1201 agagcatcct accgatgtta ctgtatctgc ggattcagcc ggagcagtat ttaactggct
     1261 gttcgtgcac gaggaccgtt cggatggcgt ccataatacc gaatatgctg caggtctgct
     1321 gcaagctgca atcaatttca tgacgactgg agatcctgaa ggagtgccga caggaaacaa
     1381 gaaaagaagc ttcgcaaatt cgagataatt tgcttatatt acagccctcc cgtaatgaaa
     1441 cgggagggca attttatatg tatgctaaaa ctcagattac caaggctggc ttataatttc
     1501 atcagctccg taggttcggc catcgcgatc gttgcaggcc tgatggttgt gaccatgctg
     1561 gtggtgagca ttttcacgga aatagaaaat ccttacctcg gaatcttttc cttcatgatc
     1621 ctgcctgcaa tcctgatttt cggcctgatc ctgatacctg tcggcatgtt gcggcagtgg
     1681 cgccgtgaga aacggggtga gagggttgaa aagacaagat ggccctatgt ggacctgaat
     1741 ctcaagagcc accgcaatgc cttcttcatc tttattttcg gcactttctt tttcgtggtt
     1801 tttaccgcgg tcggaagcta ccaggcattc catttcagcg aatcggtcaa attctgcgga
     1861 accacctgcc acagcgtaat ggagccggag cataccgcct atcagaattc tccccatgcc
     1921 cgcgtggcct gtgtagaatg ccatgttggc cccggggcgg gctggtacac caaatctaaa
     1981 ctctccggag cgtaccaggt gtacgcaaca ttgctcaata aatatccgcg ccccattccg
     2041 acgccgatag agagcctgcg gccggcgcgg gaaacttgtg agcaatgcca ctggccggcc
     2101 aagttcttcg gcggcatgca gaagaaaatc gaccattata tgtatgacga agaaagttcc
     2161 tactggccca taaacatgct gatcaaagtc ggtggaggaa agcggggggc taatcaggct
     2221 agcggaattc actggcatat ctccagcgac gtcaaagtgg aatatatcgc ccgagaccat
     2281 cagcgcgagg acattccctg ggtgagatac accaacctcg agaccggtga ggttactgtc
     2341 tatcatgatg atgatgatcc actcagccag gaggagatag attcactcga gccccgcgtt
     2401 atggactgcg ttgactgtca caaccgtccc agccatattt ataatcctcc ggatcgagcg
     2461 atagatgatg cactcaatac ccggatgatt gaccccgaaa tcccggaaat caaaatggtc
     2521 gctgttgaag ccatggccga gaactatggc tcggatgagg aagcaatgga aaaaatccga
     2581 gaacacatca atggatatta caaagaaaat tattccgggt tttacgaaca ggaaaagggc
     2641 ctcatagaac aggcaatcca gagtaccagt gagaaatttt ctttgaacat atttccgcgt
     2701 atgaaggtgc gctgggatgt ttaccccgac cacattggcc atttctattt caagggctgt
     2761 atgcgctgtc atgaaggcaa tcattccagc gatgagggcc agactatctc acacagctgc
     2821 aattcctgcc acataattct tgcccagggc agcggcgaaa cctacgaggt tacctaccag
     2881 gaagatggac tggagttcaa acatccagtg gatatttatg aagcctggcg cgagatgggc
     2941 tgttatgaat gccatacggg tacgcagccc tgagttctta tagtatcttt tctaatgaaa
     3001 ttcagaattt aatgtttctg ccgggactca atccttttga ccttcaccca gatcgcgaca
     3061 gccaggatca acagcaccac gacagaaatg gccagccctt tgcgcctgaa gacatagttg
     3121 tccagaagtt cacgggcaat attccgggct tcagcgacct gctcaaaacc ggcagcggtc
     3181 atattaacca ccgaatccgc ctggaaagta tgcactgcag tcctcgcttt gaaagctctc
     3241 tggcgcgcat ccttcaagac aaacaggccg ttgttaacaa acaagccctt ttgatcggcc
     3301 tcctccagcc tgccctcggc ctcggcgata gcattttcaa ggctgtctat cctgctcgcc
     3361 atcaatttgg cggcctcaag cgcacctgta ccgtcatcct caaaatgaca ttgaccgcag
     3421 acaggctcct ccccgctcaa catctcagtg ccgggcgctt caatcagatg gttggagtgg
     3481 caggtttcac aggccggcga ttccagaaca gaaaaataat ccgcatgtgt ggatttttca
     3541 aaatctacca tgttgttgtt atggcagagc ccgcagactt tatcaatgga ttcggcatcg
     3601 ggtgggattg cgccgtgatt tccatggcag tcattgcagg ccggcgcacc cagatcgtca
     3661 tcttccaata gcgcggcccc atgaacggac ttcctgtaat cctccacctg gtcagtgggt
     3721 atatcgtaag gggccatata actgctgtca ttatggcagc tggcacaggt agctgatata
     3781 ttc
//
LOCUS       DSEB01000440            2627 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1005528, whole genome shotgun sequence.
ACCESSION   DSEB01000440 DSEB01000000
VERSION     DSEB01000440.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1005528"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..2600)
                     /locus_tag="ENO07_07915"
     CDS             complement(<1..2600)
                     /locus_tag="ENO07_07915"
                     /inference="COORDINATES: protein motif:HMM:PF01364.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01934.1"
                     /translation="MEYQIVSSLWSDPFSIEYADPGNKGFSAEPLEIRPPARIRSQNS
                     QSLNIYPLIIENGRGRLLIEMTVSVKFNAPADAFDKSFGFSDEGAFENLLSGLLLNYE
                     QARSWREAARPENIALQARENIFDKYQNWVKLDFGQTGIQKITSQDLSSAGIDVGTLD
                     PRLFRVFWAGGKPLNVEIGGPYPQLNEIAIHVKGEADASFDPEDYIAFYLPSANYYYY
                     DTAAAELNYTHNHYTPYGTVFMSLGPGGDSDPLRMDTVIVTPRNSGQGVSGFTDKRRY
                     EQNNFLLDINGTIFDYYSWYWREGNSFEQFINIEDREPIEHLNRLDIQVAGRSPHIEI
                     NGFEPDSSNVVDGPYVTRGSFWSYRFASGLNSLMITINNNIRGDHVLDFLELEYRRQL
                     RYRGGTFTFYGQTDPGVYHYNVNAITADSTYVICDATDIFNQKLLIRAVKEASSRTLT
                     FEYDQADPGFHRFALAEEASLGSPADVRPMTVANLIDPGQSYDLIVIAPEEFLPSFGA
                     YGTLREADGYSVYLASAEDVYAHFSGGMFDPVAIRDFLHHAFEHWPEPSPSFVVLGGD
                     GIYDYMGYTGTARQNFIPPFVVEGDLTVSDENYILFDNGGWLDSDSSYPADSGPDMIV
                     GRWPVRNAAEVTNFIAKLALYEQGSNLGSWQNSLTFVADDENKPGVSYKEPDHTYQAE
                     NLANLYSPDRFDKNKIYLIEYELDSKGEKPGAKRKLIESINDGTLLVNFVGHGNPRLW
                     TDERIFRNEDIPSLHNEEKLTVVMAASCSIGEFDSPYDEGMAEIFFRYNDGGAIASVA
                     TTRLVYSGPNADFNYKLFEVLFSDQGFSLAEAVYVAKFLRQQASMPSENDKKFIMFGD
                     PVMRLAAPQY"
BASE COUNT          651 a          677 c          645 g          654 t
ORIGIN      
        1 ctgtattgcg gcgcagccag tctcatcacc ggatcgccga acatgataaa tttcttgtcg
       61 ttctcgctcg gcatggaggc ctgctgcctg aggaatttgg ccacatagac cgcctcagcc
      121 agggaaaagc cctgatccga gaagagcacc tcgaaaagtt tatagttaaa atcagcattg
      181 ggcccgctgt aaaccagccg tgtggtagcc acggaggcga tagcgccgcc gtcattataa
      241 cggaagaaga tttccgccat accttcgtcg tagggactgt cgaattcacc tatcgagcag
      301 gaggcggcca tcacaactgt cagcttctcc tcgttatgca gggaggggat atcctcattt
      361 ctgaatatgc gctcatctgt ccagagccga gggttgccat gcccgacaaa attgaccaga
      421 agcgtgccgt cgttaatcga ctctatcagt ttacgcttcg cgccgggctt ctcgcctttg
      481 gaatcgagct catattctat cagatatatt ttgtttttgt caaatctgtc aggggaataa
      541 aggttggcaa gattctcggc ctgatatgtg tggtcgggtt ccttatatga tacccccggc
      601 ttgttttcgt cgtcggccac aaacgtcagt gagttctgcc agctgcccag gttgctcccc
      661 tgttcgtaga gggccaattt ggcgataaag ttcgtgactt cggcggcgtt tctgacgggc
      721 catctcccga cgatcatgtc cggcccgctg tcggcaggat agcttgaatc ggaatccagc
      781 cagccgccat tatcaaacaa tatataattt tcatccgaaa ctgtcagatc gccctctacg
      841 acaaagggcg gaatgaaatt ctgtctggcg gtgccggtgt aacccatata atcgtagatt
      901 ccatctcctc ccaggaccac gaatgagggt gagggctcag gccagtgctc gaaagcgtga
      961 tggagaaagt ccctgattgc caccgggtcg aacatgccgc ccgaaaaatg ggcataaaca
     1021 tcttccgccg aagcaagata aaccgagtag ccgtcagctt cgcgcaatgt cccgtaagcg
     1081 ccgaaggaag gcagaaattc ctcgggagcg attacaatca gatcatagct ttgaccggga
     1141 tctatcagat tagccacggt catcggtcgg acatctgcag gacttcccag cgaggcctcc
     1201 tctgccagcg cgaatcggtg aaatcccgga tcggcctgat catactcaaa ggtcagcgtc
     1261 ctggaagacg cttccttgac cgcgcgtatc agtagtttct ggttaaaaat atctgtcgca
     1321 tcgcaaatga cataagtgct gtcagcagta atggcattta cattgtagtg ataaacgccc
     1381 ggatctgtct gaccgtagaa tgtgaaggtt cctccacgat agcgcagttg cctcctgtat
     1441 tccagttcca aaaagtccag gacatgatcg ccgcggatat tgttattgat ggttatcata
     1501 agtgaattca atccgctcgc aaaacggtaa ctccagaatg atccccttgt tacataagga
     1561 ccgtcaacaa cattagagga atccggttca aagccgttta tctctatatg aggagatcgt
     1621 ccggcgacct gtatgtccag ccggttgaga tgctctatag gctcccgatc ctcgatgttt
     1681 atgaactgct cgaagctgtt gccctcacgc cagtaccatg aataataatc aaatattgta
     1741 ccattaatgt cgagaaggaa attattctgt tcgtatcttc ttttatctgt aaaaccgctg
     1801 accccctgcc ccgagttgcg tggtgtgact attacagtat ccatgcgaag cggatcggaa
     1861 tcaccgcccg ggccgagtga cataaacacc gttccatagg gagtataatg gttatgggtg
     1921 taattcagtt cggcagcggc agtatcgtaa taataataat tggctgaggg aagatagaaa
     1981 gcaatataat cctccggatc aaaactggcg tcagcctcgc ctttgacatg aatggcaatt
     2041 tcattgagct gtggataagg cccgccgatc tcgacattca acggtttccc acccgcccag
     2101 aaaacgcgaa acagacgcgg atccaacgtg ccgacatcaa ttccggccga gcttaaatcc
     2161 tgggatgtga ttttctgtat cccagtctgg ccaaaatcca gcttgaccca attttgatat
     2221 ttgtcgaaaa tattttctct ggcttgaagc gctatattct caggacgggc tgcctctctc
     2281 cagctgcgcg cctgctcata attcaatagc aagcccgaca ggaggttctc gaatgctcct
     2341 tcatcggaga atccgaaaga tttatcaaat gcatccgcag gagcattgaa cttcaccgat
     2401 acggtcatct caatcagaag cctgcccctg ccattttcga ttatcaacgg atatatgttc
     2461 agcgactggc tgttttgcga gcggatccgg gccgggggtc ttatttccag cggttctgcc
     2521 gaaaatcctt tattgccggg atcagcgtat tcaatcgaga acgggtcgga ccacagtgat
     2581 gaaacaattt gatattccaa tcgtgcctcg aagggggcgc caagtac
//
LOCUS       DSEB01000441            1935 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1010864, whole genome shotgun sequence.
ACCESSION   DSEB01000441 DSEB01000000
VERSION     DSEB01000441.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1010864"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            41..208
                     /locus_tag="ENO07_07920"
     CDS             41..208
                     /locus_tag="ENO07_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDS01935.1"
                     /translation="MTVKEAAKYLGVNPETLRRWDNKGKLKTRRHPMNNFRIYSKADI
                     ERIKKKISGGE"
     gene            211..1431
                     /locus_tag="ENO07_07925"
     CDS             211..1431
                     /locus_tag="ENO07_07925"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00239.19,HMM:PF13408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDS01936.1"
                     /translation="MRCAVYSRISSDMQKERSIEDQVRNCMDFAHKKGWKVLENHIYA
                     DKAVSGASLAGRIQLARLLEIASTKPTPFDYILVDDTSRLSRDKIDQMNIIEDFHTAG
                     VFLFFVSQNIDTADEQANDVVIPIHGIVDALYLKELAKKTKRGMTGQVLSGFNPGGMT
                     YGYSYTKVPDPSGRKDKKTKEVRSLGTKITVNEDESQIVRKIFALYAAGVGLKEIAHS
                     LNKDGIEPPRTDSQRGGYKVKPSWCPNAIRYMLRNPKYIGDWTWNKYKWFKNRRTGKR
                     ARRLRDKAEWVRSPRPDLRIIDDSTWQLVQNRIEANKHQKKSGRRPGSKYLLTGMLKC
                     SVCGSNLISVHVGKSDVKFACSLNWHRGPSVCPNSHKIMKSELEGKLLGAVRDQILRP
                     EMLDRLKIKIQNLA"
     gene            1564..1878
                     /locus_tag="ENO07_07930"
     CDS             1564..1878
                     /locus_tag="ENO07_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01937.1"
                     /translation="MVEKERRISELKDKWALRDAVKHLPKIDFDRKLIAGKLSELQRV
                     LKKDVGAARIGLKRFIDQFELEPLVRSGNKCYRARAKIKLQGLLGVTPETATLKNSGG
                     WI"
     gene            complement(1860..1933)
                     /locus_tag="ENO07_07935"
     tRNA            complement(1860..1933)
                     /locus_tag="ENO07_07935"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(1897..1899),aa:Met,seq:cat)
BASE COUNT          618 a          386 c          510 g          421 t
ORIGIN      
        1 atgtagtatg acaaaaaaga aaacgcgaat tcaaggttac atgaccgtaa aagaagcggc
       61 caagtacctg ggagtaaatc cggagaccct tagacggtgg gataacaagg gaaaactcaa
      121 gaccaggcgg caccccatga acaatttccg gatttattct aaagctgata ttgagaggat
      181 taagaagaaa atcagcggcg gtgagtaatt atgcgatgtg cagtatattc acgaatttcg
      241 agtgacatgc agaaagaacg atcaattgaa gatcaggttc gcaactgcat ggatttcgcc
      301 cataaaaagg ggtggaaagt tctggagaat catatttacg ccgacaaggc tgtttccggt
      361 gcctctctgg ccggcagaat tcagctggcg aggttgcttg aaattgcatc caccaaaccc
      421 acacccttcg attatattct cgtagacgat acatcgagac tctcaaggga caaaatagac
      481 cagatgaata tcatcgaaga tttccatacc gctggggtat tcctattctt tgtctctcaa
      541 aatatcgata ctgccgatga acaggcaaat gatgtggtaa tacccattca tggtatagtt
      601 gatgcccttt acttaaaaga gctcgccaaa aaaacaaaaa gaggtatgac cgggcaggtg
      661 ctgtctggtt tcaatcctgg aggcatgaca tatggctaca gctacacaaa ggttcctgat
      721 ccgtccggca gaaaagacaa gaagaccaaa gaagtgagga gcctcgggac gaagatcaca
      781 gtgaatgagg acgaatcgca aattgtaaga aagatctttg ccctatatgc cgcaggagtc
      841 ggattgaagg agattgcaca ttcgcttaac aaagatggga ttgagccccc gaggactgac
      901 agccagaggg gaggttacaa ggtgaaaccg agctggtgcc cgaatgcgat aagatatatg
      961 ctgcgaaatc caaaatatat cggggactgg acttggaata agtataaatg gttcaagaac
     1021 aggcgcaccg gtaaacgggc caggcgcttg cgcgataaag ccgaatgggt aagaagccct
     1081 cgtccagatc tgagaataat agacgacagc acctggcagc ttgttcagaa tagaatcgaa
     1141 gctaataaac accaaaagaa gagcggtaga aggcccggat cgaagtattt gctgaccggg
     1201 atgcttaagt gttcagtttg cggctcgaat ctgatttctg tgcatgttgg taaaagtgat
     1261 gtcaagttcg cgtgttcgtt gaactggcac agggggccgt cggtctgccc aaattctcat
     1321 aaaatcatga agtccgaatt agagggcaaa ttattaggtg cagtcaggga tcagatactt
     1381 cgaccagaga tgctcgatag gctaaaaatc aagatacaaa accttgcgta gaagcagatg
     1441 atgagcaatt gcgatagccc agaggtaatc cgtaaggaaa tgaagaagct cgaaaaggaa
     1501 atcgataatc tgatcgaccg tatcgccggg aatggagatc cgaccggtcg catcaataaa
     1561 aaattggtcg aaaaagagag aaggatttct gagcttaaag acaaatgggc cctccgggat
     1621 gcggtgaagc atctgccaaa aatcgatttt gatagaaagt taattgctgg aaagttgagt
     1681 gagctgcaaa gagtccttaa aaaagatgtg ggcgctgcca ggatcgggct caagaggttt
     1741 attgatcaat tcgaattgga gccgcttgtg aggtccggta ataagtgtta tagggccagg
     1801 gcaaaaataa aactccaggg attacttgga gttacaccgg agactgccac tttaaaaaat
     1861 agcgggggct ggatttgaac cagcgacctt cgggttatga gcccgacgag ctaccagact
     1921 gctccacccc gcatc
//
LOCUS       DSEB01000442            1849 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1012091, whole genome shotgun sequence.
ACCESSION   DSEB01000442 DSEB01000000
VERSION     DSEB01000442.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1012091"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..396)
                     /locus_tag="ENO07_07940"
     CDS             complement(<1..396)
                     /locus_tag="ENO07_07940"
                     /inference="COORDINATES: protein motif:HMM:PF00069.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HDS01938.1"
                     /translation="MKADENNGGYTRPYRVLVAGSMVKNYRIISRIGRGGMGEVYLAE
                     DTSLNRMTALKFLLPHICQDAGSRSRFKREAQASARLEHANIATIFEVDEFDGRPFFA
                     MQYIEGLPLNEYFKDSRIAFDELIDISSQI"
     gene            complement(556..1755)
                     /locus_tag="ENO07_07945"
     CDS             complement(556..1755)
                     /locus_tag="ENO07_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDS01939.1"
                     /translation="MKKVLIYSILLIAGLIISQYLGELGAGFFKPATEIIRLLTVFCL
                     GFIMIHVGYEFEIDRSRLKSYAVDYGVAATAAAFPWIFVAAYFVFILTPPEAWGSFDT
                     WKESLLAGRFAAPTSAGILFSMLSAAGLSATWVFKKLRILAIFDDLDTVLLMIPLKMM
                     IVGLKWQLGVVVAIMFAQLWMAWKFMRRLKLPITWPWVMVYSAGLTLITEIVYLTSKL
                     IDEVVSVHIEILLPAFVLGCMLARPEGHDPHADDRREGHQEGPEAPNEQKVASMVSGI
                     FMLLVGLTMPPIAQVLGSQPDGSSFGWGTIAVHVILITLLANLGKMFPLFVYKKESSW
                     RERLAICIGMWPRGEVGAGVLIISLSYGIGGIMITVAMLSLALNLLLTGLFIVFVKKL
                     LAHKISQ"
BASE COUNT          474 a          487 c          447 g          441 t
ORIGIN      
        1 tatctgagat gatatatcga ttagttcatc aaatgcaatt ctgctgtcct taaaatattc
       61 gttcaacggc aggccctcga tatactgcat ggcgaagaat ggtcttccgt caaattcatc
      121 gacctcaaag attgtggcga tattagcatg ctccagccgg gccgatgcct gtgcctctct
      181 cttgaaacgc gaacggctgc ctgcatcctg gcagatgtgg ggcagcagaa atttgagtgc
      241 agtcatgcgg tttagactgg tatcctctgc cagatatacc tcgcccattc ctccccggcc
      301 gattcgggag attatcctgt agttttttac catgctgccg gcaacaagaa ccctgtatgg
      361 tcttgtgtaa ccaccattat tttcatctgc cttcataggc agacccctct ccttatgctg
      421 tgaaaatcat tcctggatgt taacaaaata aacatttcct gcaataatgc aaacccatta
      481 tgtctacttt caaaatttgt tttttacgta aaatgcagga agttatccgg gtcaggggat
      541 ccgcataatc gggatttact gcgagatttt atgggcaagg agctttttga caaagactat
      601 gaataatccg gtcagcagca ggttcagtgc cagcgagagc atggcgaccg taatcataat
      661 accgcctatt ccgtaagaaa gcgatataat cagaactccg gctccgacct cgccccgcgg
      721 ccacatgccg atacatatgg ccagtcgctc gcgccatgag gattcctttt tgtacacgaa
      781 taacggaaac atcttgccca gattggccag taatgtgatc aggatcacat gaacagcaat
      841 cgtgccccag ccaaaggaac tgccatcagg ctggctgccg agaacctgcg caatgggcgg
      901 catggtcagg ccaaccaaca gcatgaaaat cccggaaacc atgctggcaa ctttctgctc
      961 attgggagcc tcgggaccct cctgatggcc ctcgcgtcta tcatctgcgt gagggtcgtg
     1021 gccctcagga cgtgccagca tacatcccag gacaaaggcc gggagcagaa tttctatgtg
     1081 caccgatacc acctcgtcta taagtttgct ggtgagatag actatttcgg ttatcagggt
     1141 gagcccggcg gaatagacca ttacccaggg ccatgttatc ggcaacttca gcctgcgcat
     1201 gaatttccag gccatccaca gctgcgcgaa cattattgcc accacaactc ccagctgcca
     1261 tttcaatccg acgatcatca ttttcagcgg gatcatcagc agaaccgtgt cgagatcatc
     1321 gaagattgcc agaatccgga gtttcttgaa gacccatgtg gccgatagtc ctgcagcgga
     1381 cagcatcgag aataaaatcc cggccgaggt gggggcagcg aagcggccgg ccagaagcga
     1441 ttccttccag gtatcgaagc taccccaggc ctcgggagga gtcaggataa atacaaaata
     1501 tgcggccacg aaaatccagg ggaaggccgc tgctgtggcc gcgacgccat agtcaactgc
     1561 atagcttttc aagcggctgc ggtcgatttc gaattcgtag ccgacatgaa tcatgatgaa
     1621 tccgagacag aaaacggtca gcaggcggat tatctcggtg gcgggtttaa aaaatcctgc
     1681 acccagctcg cccaggtatt gagaaataat cagccccgct ataagcagta tggaatatat
     1741 cagtaccttt ttcaatccat ttccctttta tttattgccc tcaacattgt cgtcccggca
     1801 gaggttccga caatcgggaa cgtcgaaata ggtctggtcc gttctgaga
//
LOCUS       DSEB01000443            2205 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1008165, whole genome shotgun sequence.
ACCESSION   DSEB01000443 DSEB01000000
VERSION     DSEB01000443.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1008165"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..701)
                     /locus_tag="ENO07_07950"
     CDS             complement(<1..701)
                     /locus_tag="ENO07_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDS01940.1"
                     /translation="MREQLMQTTEATEVKVEAKLIPIYIMGQKYEVPETLTIMKAMEY
                     SGFKFIRGCGCRGGICGACGTVYRLPGDYHIRVGLACQTVVVPDMYLTQIPFYPANRA
                     VYDMETALPELAEVQKKYPELFRCVACNACTKVCPMDVPVLDYVSAIKQGDFKKAMEL
                     SFDCIQCGLCATRCMAEMAQYHIAQMVRRLYGAKLAPQPKHAAEMVEQIKNGKYTDML
                     ADLKSKELDELKKLYT"
     gene            complement(724..1605)
                     /locus_tag="ENO07_07955"
     CDS             complement(724..1605)
                     /locus_tag="ENO07_07955"
                     /inference="COORDINATES: protein motif:HMM:PF02754.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase, subunit B"
                     /protein_id="HDS01941.1"
                     /translation="MKIAYYPGCTLKTSAKNLNDSAVAAMAKLGVELVELDRWNCCGA
                     VYSLADDDLLHQVAPVRDLIRVKDMGYDKVITVCSMCYNTLARANNLVRNDEEKRYTL
                     NSFMEEETDYNGEVEVVHLLTFLKDEIGWGKLKEKVTKPLSDLKISPYYGCTLTRPRD
                     IAIDSADNPRIMHDFLEALGAQVVNNPAGIECCGSYQILSNPDAAYSNINKIISTAIN
                     RGAEAIASSCPLCEFSLGKRQKQATEKFGDGKTLPTFYFTQLLAVALGVDDALMHFEI
                     GHEKSRELLASKNLVGA"
BASE COUNT          535 a          535 c          524 g          611 t
ORIGIN      
        1 gtgtagagct tcttgagctc atccagttcc tttgatttca gatcagccag catatcagtg
       61 tacttgccgt ttttgatctg ttcgaccatc tcggccgcat gcttcggctg gggcgccagc
      121 ttggcgccgt aaagccggcg caccatctgg gcgatatgat actgggccat ctcggccata
      181 cagcgtgtgg cacagaggcc gcactggatg cagtcgaacg acagctccat ggccttcttg
      241 aaatcgccct gctttatggc ggagacataa tccagcaccg gaacatccat aggacaaacc
      301 ttagtacagg cgttgcatgc cacacatcgg aaaagctccg ggtatttctt ctgcacctct
      361 gccagttccg gcagggcggt ctccatatca tagactgcgc gattggccgg gtagaaaggt
      421 atctgggtca gatacatatc cggcacaacc accgtctggc aggccaatcc cacacggata
      481 tggtaatcgc cggggagacg atagacagtt ccgcaggcgc cgcagatacc gccgcgacat
      541 ccgcagcccc ggataaattt aaagccggaa tattccatag ccttcataat ggtcagggtc
      601 tccgggacct catatttctg gcccattatg tagattggaa taagcttggc ttctaccttt
      661 acttcagttg cttctgtagt ttgcataagc tgttctctca tctattttaa gttcatcttt
      721 tttctatgcg ccgaccagat ttttgctggc cagaagttca cgggactttt catgcccgat
      781 ctcgaaatgc atcagagcgt catcaacacc caacgccacc gccaggagct gggtgaaata
      841 aaatgtcggc agagttttgc cgtcgccgaa tttctcggtt gcctgtttct ggcgtttgcc
      901 caggctgaat tcacatagag gacatgacga ggcaattgcc tcagctccgc ggtttatcgc
      961 ggtggaaata attttgttaa tattgctgta ggccgcatcc ggattggaga gaatctggta
     1021 tgatccgcag cattctattc cggccggatt attcaccacc tgcgcgccca gagcttcaag
     1081 aaaatcatgc atgatcctcg gattatcggc gctatcgatt gctatgtctc tgggacgggt
     1141 gagggtacag ccataataag gtgaaatttt gagatcggat aatggcttgg tgactttttc
     1201 tttgagcttg ccccagccga tttcatcttt gagaaaagtc aggagatgca caacctcgac
     1261 ctcgccgttg tagtccgttt cctcctccat aaagctgttc agggtgtagc gcttttcctc
     1321 gtcgttgcgc acgaggttgt tggcgcgcgc gagagtgtta tagcacatgg agcaaaccgt
     1381 gatcaccttg tcgtagccca tatccttgac acggatcagg tcacgaaccg gagccacctg
     1441 gtgcaggagg tcgtcatcgg ccagtgaata gactgcaccg cagcagttcc agcggtcgag
     1501 ttcaaccagc tccactccca gcttggccat cgccgcaacc gcagaatcat tcaggttttt
     1561 ggcgctggtt ttgagtgtac atccgggata gtaagcgatt ttcatgatgc tgttctttct
     1621 atagcttgat attatttttc gatgtaaaga gtaacgttag catgtgaatt atttcacctg
     1681 caaaattgct taaaaaaatt tttgaaagta gttttttctc ttatttccgc ctgcgcgcct
     1741 cacggctcgc gtataagaca gtatttataa agagattaac catatatgtc aagtgatttc
     1801 attcacttaa tctttccggt cttcccctat tatagttatt cgtataaact taagtattcg
     1861 tttgaattat gaggaaattc gcatgattta ttattttggc cgatttataa attatatagg
     1921 catctaaata tagccaaagg tggatttcca caaatggcta tgattcagga tataagcgca
     1981 cggatctata ccggtaacta ccgaaaaaac cctcggcttt tccggaaaga ttattggact
     2041 cctgaaccag aattctgtcg ccatatataa tcccctgctg ataacagaat actcgtatga
     2101 ttataaaatg ccccgacagg atatcagggg gaacagtaat agtggtatcg gtcaggggat
     2161 cggatatata cgcaggcacc gcaaattccg gatagagttt tatga
//
LOCUS       DSEB01000444            5578 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001029, whole genome shotgun sequence.
ACCESSION   DSEB01000444 DSEB01000000
VERSION     DSEB01000444.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5578)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001029"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(183..619)
                     /gene="tnpA"
                     /locus_tag="ENO07_07960"
                     /pseudo
     CDS             complement(183..619)
                     /gene="tnpA"
                     /locus_tag="ENO07_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_007001361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(786..2591)
                     /locus_tag="ENO07_07965"
     CDS             complement(786..2591)
                     /locus_tag="ENO07_07965"
                     /inference="COORDINATES: protein motif:HMM:PF17210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01942.1"
                     /translation="MSKLRILLPLLAILGLIFVGCQGGENFSTSGNSLNLTNKATVHT
                     ETLGIPDIEIEDGSGLVVEGTGMLTQPASIEIEVPSGDIEQVLLYWSGGTNVEPLEGD
                     DIIVFEGVEIQGQLIGGPAFFFEDYDFYAYRADITELELVSTGTNTYSVDGMEFAFEG
                     DNQNSGVALVVIYDDGSEADIQLREGLDLAYYDFPEPRNAVVPQTFTFLPSSMPRPAR
                     VFLAAGSAEESSASKVVITVGDAVYEFDDVFGSNDGLLWDTEMIAVSVPAGVTSLTVE
                     VMSMDSEDPPAAHLSWVMAAMSIPLTQVVQVASVGDLVWYDMNMDGIQDEGEPGIEGV
                     EVSLYDCEDTFIASTMTDSEGMYMFGELMPGDYYVMFELPDDHVFTMMDQGMDDAMDS
                     DADPETGKTMCFTLESGETDLTWDAGLYMEEVDDGCTRSKGYWKNHAGFGPQEDVVTQ
                     YLPIWLGNDDGDKSFAVTDARIAVDILHQHTYGEPSNGITKLYAQLLAAKLNIAAGAN
                     DDAVEDVITDADDFLSMYDWEDWYDLGWEDRETVLYWKDMLDSYNNGHIGPGYCGDFD
                     DDDYDDDDDDDDDDNDDDDNDDNDDPDKGFEYSDE"
     gene            complement(3322..3786)
                     /locus_tag="ENO07_07970"
     CDS             complement(3322..3786)
                     /locus_tag="ENO07_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase"
                     /protein_id="HDS01943.1"
                     /translation="MADEPKTENCENPEECTGKVGFVISEKTFGREFVEKVKEISGEN
                     IDKCMQCGTCSGGCPMIDVLQITPRGIMHLSALGQEQKIAKANTIWMCASCNTCAVRC
                     PRGIDIPKVMEAMRQIFLRKNVNYIEPNQIPDEVLEDMPQIVMVSCFRKHTA"
     gene            complement(3799..5469)
                     /locus_tag="ENO07_07975"
     CDS             complement(3799..5469)
                     /locus_tag="ENO07_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDS01944.1"
                     /translation="MPYPEALKELIKKVEETRPARVEKKKAGEEYPMMSLEERAEILK
                     FHPDYIDEGRREIKIGPNKGYKIAHEMVDLIEARSRVNPEKVDLTKWDYETDVLVIGG
                     GGAGTSAALLAQEQGAKVIIATKLRHGDANTMMAEGGIQAATKGWKDSPFYHYLDVMG
                     GGHFVNKPELVETLVTEAPDVIKWLCEMGCNFSRYDDGTLKTMHGGGTSRKRMHYAAD
                     ITGAEMMRTVRDEARNRIDDITVLEFSPAVELIKNEHGECAGALLYNMETREYFIVKA
                     KAVVMATGGSGRLHVQNFMTTNHYGATADGIVMGYRAGVGLCFLHTIQYHPTGVIFPE
                     QAEGILITEKFRGSGANLVNIHGEQFVNEREPRDIEASAIIRECVDRGQGVPTPTGKF
                     GVWLDSPMIDELSGEGTVEKEFPGKFILYKRFEIDISKEPMLIYPTLHYQNGGLEFEK
                     TGETKLPGFYVAGEVGGGTHGENRLMGNSLLDIMVYGRIAGKNAAQFAKEKARDGKLN
                     LDHVAKYHKEMEEAGIETDRVAPMLLPDYTPENIKERELTSHYVGTMR"
BASE COUNT         1309 a         1531 c         1277 g         1461 t
ORIGIN      
        1 ctataatcta gaaaatgtct gcagtataat caagaattaa cacaatcatt gaatcaagaa
       61 ggtagtaata tgtgttttgg gtgggcattg ctggcaaact aaacgtacgc aagggcagct
      121 accgatcgta tgacaagtaa attatcaccg gccacagggc cggtggcttt tatcctaccg
      181 acttaagtcg gcttatacat atccagctgt atctggttat gtttgctctg gtgcctcaca
      241 tagcgtttta tgacctcttc atcgacacca cctgtgctta caaaatatcc tcttgcccac
      301 aaactcccct tgttgcataa ctccttgtat ttcttattct tcttcttgat ccacgccgaa
      361 ctcttaccct tgatgataga catcgcatat gaaaccgaat gcttcggtgg aatgatcaaa
      421 accaaatgaa tgtgatctga acaaatcctc ccctctatta tctcaatccc ttccacttac
      481 agacaagtct tattatcctc cttatctcct tggctacttg accttttaaa agttttcctc
      541 gatatttcgg cgtaaatact atatgatact tacaatcata gaagctgtgg gataaagtcc
      601 gtatatccct acgtctcaat aaatgtcacc tcctctctga ggtggcattc tattttatta
      661 cagcgaattt ggcaatgctt cgctttttaa cgtacacacc gggtaaaccg gtggtgttta
      721 gcataaaaaa aacccggcag atcattaatt ctgccgggca tattaagacc ttatcttaag
      781 gcttcttact catcactata ctcgaaacct ttgtccgggt cgtcgttgtc atcgttatca
      841 tcatcatcgt tatcatcgtc gtcgtcgtca tcgtcatcat catagtcatc atcatcaaaa
      901 tcgccgcaat aacccgggcc gatatgtccg ttattgtagg aatccagcat gtccttccag
      961 tacaggaccg tctcacggtc ctcccagccg agatcgtacc aatcttccca gtcatacatg
     1021 gacaggaagt catcggcatc cgtaatcaca tcctcgacag cgtcatcatt ggcgccggcg
     1081 gcaatgttga gcttggccgc aagcaactgg gcataaagct tggttatgcc gttactgggc
     1141 tcgccgtatg tatgctgatg gagaatatct accgcaatcc gggcatcagt gaccgcgaag
     1201 ctcttatcgc catcatcatt acccaaccag atcggcagat actgtgttac cacgtcctcc
     1261 tgcgggccga agccggcatg gtttttccag taacccttgg aacgagtgca gccgtcatca
     1321 acttcttcca tgtagagtcc ggcatcccag gttaaatctg tctctccgga ttcgagagtg
     1381 aaacacatgg tcttgcctgt ctcaggatcg gcatcgctgt ccatggcgtc atccataccc
     1441 tgatccatca tggtaaagac atgatcatcc gggagctcga acataacata gtagtcgcca
     1501 ggcatcagtt cgccaaacat atacatgcct tcggaatcag tcatggttga ggctatgaaa
     1561 gtatcctcgc agtcgtagag actgacctca acaccttcga ttcccggctc gccttcatcc
     1621 tgaataccat ccatattcat gtcataccag actaagtcac cgacggaagc gacctgtacg
     1681 acctgcgtaa gcgggatgga catggcagcc ataacccagc ttaaatgggc ggcaggggga
     1741 tcctcgctgt ccatcgacat aacctctacg gtcaatgacg tcacaccggc gggcacagaa
     1801 acggcaatca tctcggtatc ccacaacagc ccgtcgttgg agccgaagac atcatcgaat
     1861 tcataaacag cgtctccaac tgtgataaca accttgctgg cgctgctctc ttcggcgctc
     1921 cctgctgcga gaaatacccg ggcgggcctg ggcatcgaag atggcaggaa ggtgaaggtc
     1981 tgcggtacta ccgcattgcg cggttccgga aaatcgtagt aagccagatc aaggccctcg
     2041 cgcaactgga tgtcggcctc gctgccatca tcatagatca ctacaagggc aacaccgcta
     2101 ttctgattat caccctcaaa ggcaaactcc attccgtcca cggaataggt gttcgtcccg
     2161 gtggagacaa gctcgagctc ggtaatgtcg gcgcgataag cataaaagtc gtaatcttca
     2221 aagaagaagg cgggtccgcc gataagctgc ccctgaattt cgacaccctc gaaaactatg
     2281 atgtcgtcgc cttccagagg ctcgacattg gtaccacccg accagtacag aagcacctgc
     2341 tcgatatcac ctgatggtac ctcgatttca atcgaagccg gctgggtcag catgccggtg
     2401 ccctcaacca caagccccga accatcctca atctcgatat cggggatgcc cagggtttcg
     2461 gtgtgaactg tagccttatt cgtgagatta aggctatttc ctgaggtaga gaagttttcg
     2521 ccaccctgac agccgacaaa gatcaggccc aggatcgcga gtaagggaag tagaatgcgg
     2581 agttttgaca tgaatactcc tcctttttag gttagtttaa tatgcggatt ttatccacct
     2641 attattttat gcacctatta taaatgcccg aaaagcgagt aaatacgggc ttttgccaat
     2701 atatataagt aaaatgtcaa atgccagaat tagttccaat aaaacattat ttggatagga
     2761 tagtgcatga tgatggtcag aagatggtcg aaaattattt tgttaatatg gctttcttgc
     2821 tcttgatgta gcgctttaag tgatattgct atattattat catacaatgc actgcagatc
     2881 agccacaagg ctgtaggtca attcctgaag ggaattgacg attaaaatag gctgtcaagc
     2941 cgcttcaaag cttgacctgc tgaaaacttg aatttggaaa gacagccaga accgctgctt
     3001 tgcactcccg gttcttccat acaaaaatcc cggcggacgg ggatatccgc caggcactat
     3061 attcattcca tcgtgtcatt ctgaacgaag tcgaaggtcc ggccgtcgac agatgacgtg
     3121 tctgcttgat tcgttaaaga ttcttcgtcg cttcgctcct cagaatgact ttatattcgt
     3181 gcgcggcccg tcagaggtcg ggcaagcgta tctcctcatg cgccgcgtcc actgggtgca
     3241 cccggaggta cgcccagcac gaagtcttta atattcgtgc gaggagtacg tcctcgtgct
     3301 cctcgcctct ctttctattg tctacgctgt gtgcttgcgg aagcagctta ccatgacgat
     3361 ctggggcatg tcctccagaa cctcatccgg aatctggttg ggctcgatgt aattcacatt
     3421 cttgcgcagg aatatctggc gcatagcctc catcaccttg gggatatcga tcccgcgggg
     3481 acatctgacc gcacatgtgt tacatgacgc acacatccag atcgtattgg ccttagcaat
     3541 cttctgctcc tggcccaggg ccgagagatg catgataccg cgcggggtaa tctgcagtac
     3601 atcaatcatc ggacatccgc ccgaacaggt cccgcactgc atgcatttat cgatattctc
     3661 gccggagatt tccttgacct tctccacgaa ctcccgtcca aaggttttct cggaaattac
     3721 gaagccaacc ttgccggtgc actcttccgg attttcacag ttttcagttt tcggttcgtc
     3781 agccataata gccctctctt atctcattgt gccaacataa tggctggtga gttcgcgttc
     3841 ctttatattc tcgggcgtgt aatccggcaa tagcatgggc gccacgcgat cggtctcgat
     3901 tccggcctcc tccatctcct tatgatactt cgccacatga tccagattca atttgccgtc
     3961 tctggctttc tctttggcga actgcgccgc attcttaccg gcgatacgtc cgtaaaccat
     4021 gatatccaga agcgagttgc ccatcagacg gttttcgccg tgggttccgc cgccaacctc
     4081 gcccgctaca taaaatccgg gaagtttggt ctcgccggtc ttctcgaatt cgagtccgcc
     4141 gttctggtaa tgcagggtcg gatagatcag catcggctcc ttggagatat cgatctcgaa
     4201 tctcttgtag aggataaatt tgccggggaa ttccttctca accgtgccct cgccggagag
     4261 ttcatcgatc attgggctgt ccagccatac gccgaactta ccggtaggag taggcacacc
     4321 ctgcccgcga tcgacacact cacggataat agccgaagcc tcaatatccc ggggctcgcg
     4381 ttcgttcaca aattgctctc cgtgaatatt gacaagatta gcacctgatc cacggaattt
     4441 ttcggtaatc aggataccct cagcctgttc cgggaagatc accccggtgg gatggtactg
     4501 gatcgtatgc aggaagcaaa gcccgactcc tgcacggtag cccatcacga taccgtcggc
     4561 ggttgcgccg taatggttgg tggtcatgaa attctggaca tgcagacggc cgcttccacc
     4621 cgtcgccatc acaaccgctt tggccttgac gataaagtat tcgcgggttt ccatgttata
     4681 cagaagagcc ccggcacatt cgccgtgctc atttttgata agttccactg caggtgagaa
     4741 ttccagtaca gttatatcat caatacgatt cctggcctcg tcgcggacag tgcgcatcat
     4801 ttccgcgccg gtgatatcgg cggcgtaatg catgcgcttg cggctggtac caccgccgtg
     4861 catggttttc agggtaccgt catcataacg tgaaaaatta cagcccatct cacaaagcca
     4921 tttgataacg tcgggcgcct cggtaaccag tgtctcaacc agttcgggtt tgttgacaaa
     4981 atgtccgccg cccataacgt cgagataatg atagaagggt gaatctttcc atcccttggt
     5041 ggcggcctgg ataccgccct ccgccatcat cgtgttggcg tcgccatgac gcagcttggt
     5101 tgcgataatt accttcgctc cctgctcctg cgccaagagt gcggccgagg ttccggctcc
     5161 gcctccgcct ataaccagga cgtcggtctc ataatcccac ttcgtgagat caaccttctc
     5221 gggattgaca cggcttcgtg cctcgatcag gtcaaccatc tcatgggcga tcttgtagcc
     5281 tttattcggg ccgatcttga tttcgcgccg gccctcgtca atgtagtcgg gatgaaactt
     5341 gaggatttcc gcgcgctcct ccagagacat catggggtac tcctccccgg ccttcttctt
     5401 ttcgactctg gccgggcgcg tctcctcgac tttctttatt aattccttta atgcttcagg
     5461 atacggcata atctatatct ccaattgaat gttcaaaacc tcttacaggc aggatgtatc
     5521 ctgcggtttc cagtcttccg ggctcatgtg cggctcggac tcgcgctcgg tgtagagc
//
LOCUS       DSEB01000445            1808 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1012641, whole genome shotgun sequence.
ACCESSION   DSEB01000445 DSEB01000000
VERSION     DSEB01000445.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1012641"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(185..>1808)
                     /locus_tag="ENO07_07980"
     CDS             complement(185..>1808)
                     /locus_tag="ENO07_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDS01945.1"
                     /translation="DMTARSRIRELFHEKGRLKSKVALGKEEEAAKYRDYFDWEEPIK
                     RAATHRVLAVMRGEKEGNLSFHILPAEEDAIRLLNKLFVDGNYQTLEQVRLAIRDSYK
                     RLLAPSMETEIRNEYKKIADDEAIRVFAENLRELLLSPPLGQKNILAIDPGFRTGCKV
                     VCLDRQGNPMYNTTIFPHEPDFKEEESAEAIEALCQRFRIEAIAIGNGTAGRETESFI
                     RKLDLPKEIITVMVNEAGASVYSASDVAREEFPDYDLTVRGAISIGRRLMDPLSELVK
                     IDPKSIGVGQYQHDVDQSALKQSLDDTVMSCVNAVGVELNTASKQLLTYVSGVGPHLA
                     QKIIEYRDEHGDFKTREELMEVPKFGPKAFEQAAGFLRIRDAENPLDKSAVHPESYHI
                     VNRMAKDLGCTVEDLMESEDLRKRIDLKKYMTDTIGMPTLKDIMNELAKPGRDPRQEF
                     EVFSFAENVNSIGDLKENMVLPGIVTNVTNFGAFVDIGVHQDGLVHKSQLADKYVEEP
                     STVVRVNQKVKVKVLSIDLERNRISLSMKGLK"
BASE COUNT          415 a          530 c          407 g          456 t
ORIGIN      
        1 ctgtatgaaa tcctgcccat tcagccatcc gcccgcattc gtccatgaca tagtccgcct
       61 gggaatcaaa aactcccccc tcgcccgcaa aatctctgtt tggggcgatg atttgtttta
      121 tggtaagaag gcaaaatatt gatatggaaa atatgagctg taggtcgaaa gtcagagatt
      181 taatctactt cagccccttc atcgatagcg aaatccggtt gcgctccagg tcaatcgaaa
      241 gaactttaac cttgaccttc tggttgacac gtaccactgt cgagggctcc tcgacatatt
      301 tatccgcaag ctggctttta tgcaccagac cgtcctggtg aacccctata tcgacaaacg
      361 caccgaaatt ggtaacattc gtgacgattc ccggcaggac catattctcc ttcaagtccc
      421 caatgctgtt aacattctca gcaaagctga agacctcgaa ttcctggcgc gggtcgcgtc
      481 ccggcttggc aagctcattc atgatgtcct tcagagtggg catgccgatc gtatcggtca
      541 tgtacttctt caaatcaatc cgtttacgta aatcttccga ttccatcaaa tcctcaaccg
      601 tacatcccag atcctttgcc atacggttga ctatgtgata actctccgga tgcaccgcgc
      661 ttttatccag cggattttcg gcatcccgaa tccgcaggaa gcctgccgcc tgctcaaatg
      721 ctttggggcc gaacttcggc acctccatca attcctcgcg cgtcttaaaa tcaccatgct
      781 catccctgta ctcaataatt ttctgggcca gatgcggccc caccccggag acataggtca
      841 gaagctgttt gctggcagtg tttagctcca ccccgacagc attcacgcag gacatgacag
      901 tatcatcgag gctctgcttc agggctgact ggtcgacatc atgctggtac tgcccgaccc
      961 cgatcgactt gggatcgatc ttcacaagtt ccgagagcgg atccatcagg cggcgtccga
     1021 tggaaattgc cccgcgcaca gtcagatcat aatcgggaaa ttcctcccgc gccacatcgg
     1081 atgccgaata aaccgaggcg ccggcctcat tgaccatgac tgtgatgatc tcctttggaa
     1141 gatcgagttt tcggatgaat gattcggtct cgcgacccgc ggtaccattt ccgatagcga
     1201 tagcctcgat tctaaagcgc tggcaaagag cctcaattgc ttcagccgat tcctcctctt
     1261 tgaaatccgg ctcatgcggg aatatggtcg tattatacat cggattgccc tggcgatcga
     1321 ggcagacgac cttgcacccg gttctgaatc ctggatcaat cgccagaatg tttttctgtc
     1381 ccagtggcgg cgacaaaagc agctctcgaa gattctcggc aaaaactctg atcgcctcat
     1441 catcggcgat ctttttatat tcgttacgaa tttcggtctc catcgagggc gccagaaggc
     1501 gtttgtatga atccctgatc gcgagcctga cctgctccaa ggtctggtag ttcccatcca
     1561 caaacagctt attcagaagg cgaatcgcat cctcttcagc gggcaggata tgaaaggata
     1621 aattcccctc cttctcgccg cgcatcaccg ccagcacacg atgtgtggcc gcccttttta
     1681 tcggctcctc ccagtcaaaa tagtcgcggt atttggcggc ctcttcctcc ttacccaaag
     1741 ccactttcga tttcaagcgg cccttctcat gaaaaagctc gcgtatgcgt gaacgcgcgg
     1801 tcatatcc
//
LOCUS       DSEB01000446            1832 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1012316, whole genome shotgun sequence.
ACCESSION   DSEB01000446 DSEB01000000
VERSION     DSEB01000446.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1012316"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(90..785)
                     /locus_tag="ENO07_07985"
     CDS             complement(90..785)
                     /locus_tag="ENO07_07985"
                     /inference="COORDINATES: protein motif:HMM:PF16697.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HDS01946.1"
                     /translation="MPEIIVKYDNKIIEKVVTEKPRISIGRTSDNDIVLDNRGVSRKH
                     ALIEFSPDSAIVIDNDSLNGTFVNDRKIMEETLKDKDRICIGKYELIFNSSMERENKF
                     SDLDGTMLLQTKAHKERLKADKKQKEITEKLGCSVILEVNNKGDKREHLLDRDVVTFG
                     KSKFCNIPIRGFWAPQIAAKVIREGASFTLANLGKIGYTKVNGEAISRRDLRNNDLIQ
                     IGRSFFKFIEAGA"
     gene            886..>1832
                     /gene="dusB"
                     /locus_tag="ENO07_07990"
     CDS             886..>1832
                     /gene="dusB"
                     /locus_tag="ENO07_07990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDS01947.1"
                     /translation="MSDKKLSLNGKVVSAPLANIAGSAYRAMALRYGAAIAYSEMISV
                     DGLVRGNRRTLDMLRLREGEKPVCFQIFGHDPKMIYDAARIVAGTGCDMIDINFGCPA
                     KKVVRNLSGAALMNDLELAEKLITAVVTAVDIPVSVKFRSGWNRESMSFIEFGKMAEA
                     CGASYLVLHPRTRASGFKGKSDWSKLKLLKESVGIDVIGSGDISTPEEAGRMIDETGC
                     DYVMIGRAAMGAPWIFQRIDHYLRTGDDPGEPPLDQKIEIMLEFCRLMIDDFGERAAC
                     LKLRKHLAWFTRGWHNVTRIRPLMFSVENYRDIEKILYN"
BASE COUNT          427 a          399 c          475 g          531 t
ORIGIN      
        1 gagcttattc agtccggcgg tttcgaaatc ttttaaacat gcggcgaggg cttcccagtt
       61 cccatgaaca tctgaaaaaa atcctaccct catgcacctg cctcaataaa tttgaagaag
      121 gaccttccaa tttgtataag atcgttgttt ctcaagtcac gcctgctgat tgcttcacca
      181 ttcaccttag tataaccaat tttcccgaga ttagccagtg taaaggaggc cccttcccgg
      241 atgactttgg cggcaatttg aggcgcccag aagcccctta tggggatatt gcagaatttt
      301 gactttccaa atgttaccac atcgcggtcg agcaggtgtt cccttttgtc acctttgttg
      361 ttcacttcca gtataaccga gcagcccagt ttttcggtga tctctttttg tttcttgtcg
      421 gccttcagtc tttccttgtg agcctttgtc tgcaggagca tggtgccgtc caggtcagag
      481 aacttattct cacgctccat ggaactattg aatattaatt cgtatttacc aatacagatg
      541 cggtctttgt ccttgagtgt ctcctccatg atcttgcggt cattgacgaa ggtgccgttc
      601 aagctgtcgt tatctatcac tatggctgaa tccggggaga attcaattaa tgcatgtttc
      661 ctggacactc cgcggttgtc cagtacgata tcgttatcag aagttcggcc aatggaaatg
      721 cggggttttt cggtgacaac tttttcaatt attttgttgt catattttac aataatctcc
      781 ggcatttttt gctccttgtt agtcggcacc taatataaag tcgtgcttga tcttacaggc
      841 agtttgcgct tgcctgcagg gattaataca attatattgt cggttatgag cgataaaaag
      901 ttaagtctaa atggtaaggt tgtttccgct ccgctggcca atatcgccgg ttcggcttat
      961 cgggcgatgg ctttaagata tggcgctgca atagcttaca gcgaaatgat ctcggttgac
     1021 gggctggtca ggggcaatcg caggaccctg gatatgttga gattgcggga gggggaaaag
     1081 ccggtctgtt tccagatctt cgggcatgac ccgaagatga tctacgacgc cgcccggatt
     1141 gtcgccggta ccggatgtga tatgatcgat atcaattttg gatgtcccgc caaaaaagtt
     1201 gtccgcaatt tatccggggc ggctttgatg aatgatctgg aattggctga aaagcttata
     1261 actgcggtag tcaccgcggt cgatatacca gtctcggtca agtttcgctc aggctggaat
     1321 cgtgaaagta tgagcttcat tgagttcggc aagatggccg aggcctgcgg agcgtcatat
     1381 ctggtattac atccccgaac acgagcttcc gggttcaagg gcaagtcgga ttggagcaag
     1441 ctgaagctct tgaaggagag tgttggcata gacgttatcg ggtcgggtga tatcagtact
     1501 cccgaggaag ccgggcggat gatagatgag accgggtgcg attatgttat gatcggacgg
     1561 gcggctatgg gtgctccctg gatctttcaa cgaattgacc attatctcag gactggggat
     1621 gatccgggag agccgcctct ggatcagaag atagaaatca tgctggagtt ctgccgtctg
     1681 atgatcgatg acttcggaga aagagcggcc tgtttgaaat taaggaagca tctggcctgg
     1741 tttacgaggg gctggcataa tgttaccaga atccgtcccc tgatgttttc agtggaaaat
     1801 taccgggata ttgaaaaaat tttgtacaat ta
//
LOCUS       DSEB01000447            2313 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1007335, whole genome shotgun sequence.
ACCESSION   DSEB01000447 DSEB01000000
VERSION     DSEB01000447.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1007335"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="ENO07_07995"
     CDS             complement(<1..1089)
                     /locus_tag="ENO07_07995"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00571.26,HMM:PF01595.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDS01948.1"
                     /translation="MPLLLIALAAVLFYLAFSVNVFGFGLMMYDDASEDVFKDLSERR
                     KRRLNRFFSNITRFKYFVQIFNNFALVVIALLIYRVTVHIFAQNAVVRAAITIAAIVL
                     LWLIFLTVVQFFSKRVSARWIVNTLIKKQTLMSLLYGLFTPFILVFMKTLDIYKGEKI
                     PDDKKEELVERAIESMADEVGIEEPLMEEDEKEMIENIFELDQTSVKAVMVPRIDIVS
                     LHSRMGLEEIKEIVRNSGHSRFPVYGEDADDVLGFLYVKDLFCRDAVKDEKFNIREYI
                     RKAYVIPETKRLDELLEELKRSRTHIALVVDEYGGTAGIVTMENIIEVIVGDIQDEHD
                     YEHPDIVRLESGEVRVNANVSVEDLAEYL"
     gene            complement(1091..1519)
                     /gene="ybeY"
                     /locus_tag="ENO07_08000"
     CDS             complement(1091..1519)
                     /gene="ybeY"
                     /locus_tag="ENO07_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_429468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDS01949.1"
                     /translation="MKVEVFNNTGQKYLPKKILAKTVNYILVSEDGDFDVNVVLTDSG
                     KIRALNRKFRGKDSSTDVLSFAPEESDEPPPFKPIGEIYISVPDAVRQAKEAGHSLRR
                     ELLFLAAHGTLHLCGYTHETDTKYRKMMDRTEMYLERSGR"
     gene            complement(1516..>2313)
                     /locus_tag="ENO07_08005"
     CDS             complement(1516..>2313)
                     /locus_tag="ENO07_08005"
                     /inference="COORDINATES: protein motif:HMM:PF01966.20"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDS01950.1"
                     /translation="VINAILSPIIAMGILPVFESLFNITTDLRLLELSDMNHPLLRRL
                     ALEAPGTYHHSILVGNLCEKAAEAIGANPLLARVGTYYHDIGKMEIPEYFVENTSGSK
                     SRHDTISPHMSALIIGSHVKKGLELAEEEDLPEAIMAFIAEHHGTSLMSFFYHKATEL
                     AKEEGEEEVPEMEYRYPGPKPRSKETAILMIADSVEAASRTLDEPKPSRIRSLVRKII
                     QSKFESGELSDSELTMRDLSIMENAFTQMLLGIFHKRVDYPSKEEEE"
BASE COUNT          554 a          649 c          509 g          601 t
ORIGIN      
        1 gagatactct gccaggtcct ccacggagac attggcattc acccgcacct cgcccgactc
       61 aagccgcact atatcgggat gctcgtaatc atgttcatcc tgaatgtctc ccacaattac
      121 ctctatgatg ttttccattg tcactatccc ggcggtgcct ccatactcgt caacaaccag
      181 tgcaatatgc gtgcgggagc gtttcagctc ctccagcagc tcatccagcc gtttcgtttc
      241 gggaattaca taggccttac ggatatattc acggatattg aatttctcat ccttgaccgc
      301 atcccggcag aacagatcct tcacatacag aaatcccagg acatcatccg catcctcacc
      361 gtaaaccgga aagcgcgaat gccctgaatt gcggactatc tccttaatct cctccaggcc
      421 cattcttgaa tgcagcgaga cgatatcaat cctggggacc atcaccgcct tgacgctggt
      481 ctgatcgagc tcgaatatat tctcgatcat ctccttctca tcctcctcca tgagcggttc
      541 ctcgatacca acttcgtcag ccatcgattc gattgccctc tcaaccagct cctccttttt
      601 atcatcagga atcttctcgc ccttgtaaat atccagggtc ttcatgaaga caagaataaa
      661 gggtgtgaac agcccgtaaa gcaggctcat cagcgtctgc ttctttatca gggtatttac
      721 aatccagcgc gccgataccc tctttgaaaa gaactgcacc actgtcagaa agatcagcca
      781 taaaagtacg attgccgcaa tcgtgatggc cgcccggaca acagcattct gcgcaaaaat
      841 atgcactgtc acccgataga ttaaaagcgc tatcaccacc agcgcaaaat tgttgaatat
      901 ctgtacgaaa tacttgaacc tggtgatatt tgagaaaaat ctgttcaacc gtctcttcct
      961 gcgttcggag agatctttaa atacatcctc ggaggcatca tcgtacatca taagcccgaa
     1021 gccgaagaca tttacgctga aggccagata gaataggact gccgccaggg caatcaataa
     1081 taaaggcatt ctacctccct gacctttcca gatacatctc tgtcctgtcc atcattttcc
     1141 tgtacttggt atctgtttcg tgagtatatc cgcagagatg aagggtgccg tgggcggcta
     1201 agaagagtag ctctctgcgc agtgaatgtc cggcttcttt tgcctgtctg accgcatccg
     1261 gcacactgat atatatctca ccgatcggtt taaagggtgg aggctcgtca ctttcctcgg
     1321 gtgcgaacga gaggacatcg gtcgagctgt ctttaccccg aaacttcctg ttcagcgcgc
     1381 gtatttttcc tgaatctgtc aaaaccacat tgacatcgaa atctccatct tccgaaacta
     1441 aaatataatt caccgttttc gcaaggattt ttttgggaag atacttctgc ccggtattgt
     1501 taaaaacctc taccttcact cttcttcttc ctttgaagga taatcgaccc gcttatggaa
     1561 tattccaagc agcatctgcg taaaggcatt ctccatgatc gacaggtcgc gcatggtcag
     1621 ctccgaatcc gaaagctcgc ccgattcaaa tttgctttgt atgattttgc gcaccagact
     1681 cctgatcctg gacggtttgg gctcgtccaa tgtgcgcgag gccgcctcga ccgagtcggc
     1741 tatcatcagt atcgcggttt ccttggaacg gggcttgggt cccgggtaac ggtattccat
     1801 ttcggggact tcttcctcgc cctcttcctt ggccagctcc gtggccttat gatagaaaaa
     1861 cgacatcagc gaggtaccgt ggtgctcggc aataaaggcc ataatagcct caggcaaatc
     1921 ctcctcctcg gccagctcca gccccttctt gacatgcgac ccgattatca gggcgctcat
     1981 gtgcgggctg atggtatcat gtctgctctt ggaaccgctg gtgttctcaa caaaatactc
     2041 cggaatctcc attttaccga tatcatgata ataggtaccc accctcgcca gcaaaggatt
     2101 tgcaccgatt gcctcggccg ccttctcgca gagattgccc accagtatgg aatgatggta
     2161 ggttccaggc gcctccagcg ccagccttct cagaagcgga tggttcatgt cggaaagctc
     2221 gaggagcctg aggtcggtgg tgatattgaa aagcgattcg aacaccggca ggatgcccat
     2281 cgcaattatg ggtgaaagta tggcgtttat gac
//
LOCUS       DSEB01000448            1882 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1011596, whole genome shotgun sequence.
ACCESSION   DSEB01000448 DSEB01000000
VERSION     DSEB01000448.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1011596"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            64..1626
                     /locus_tag="ENO07_08010"
     CDS             64..1626
                     /locus_tag="ENO07_08010"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDS01951.1"
                     /translation="MKGGVLTKSFDQKKIKDVAGEIDRLVEERGDHYIAFLSGLLRFE
                     TASGQKTRAGQDKFQEAIKNCLEYLRVESERLSMAFRNYDNLAAVAELKTKQKSQRSV
                     GVAAHIDVVPAGKGWRYPPFGGTVAEGCIWGRGAQDDKGPVASVYSALDALKSLGLRQ
                     VSDIRFLIGTLEETDDWPDVDLLRDKRETPDVTIVPDGIFPVINAEKGMAMLELSGTW
                     PEEKPSAEKPRFISLTSGQRHNMVPDTAVLTLLAESSQKSAIARRLTDTAVTLKEIIP
                     DSDFSMTHKEDSKNSGKTIFELSFLGKSAHGAFPEKGHNAALDALAFIDMLEYGPEGL
                     RVFAGGLSRRCGLLDGSGFGIGCHHDYMGNTTINLGVLTLTPKSGNALVNVRYPSGVE
                     SANVRAAFEKAAAEDSNALDGLKVKSRIHGRIHEALFISPDNHPEFLHSLQIAYETVT
                     GRPATLNSIRGTTYAKAFPAAVAFGPLDEKAGDIEMAHEVDERIRIDRYLENIKIYAL
                     ALALLAYNHQHY"
     gene            1659..>1882
                     /locus_tag="ENO07_08015"
     CDS             1659..>1882
                     /locus_tag="ENO07_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDS01952.1"
                     /translation="MRVTFTFNLRKSEDDEKQAERFSQEYVDRVVSALEELGHHVSPV
                     EVSGSPGEVVDKLIDSRPDLIFNLAEGVGG"
BASE COUNT          481 a          440 c          510 g          451 t
ORIGIN      
        1 atattatgtc atatacccag tattatacat cagaatgatt gagaaaacgt aaaccgctat
       61 ccaataaaag gcggcgtttt gacaaaatcc ttcgatcaga aaaaaatcaa ggatgttgct
      121 ggtgaaatcg atcgtctggt cgaggagcga ggcgatcact atatcgcctt cctatccggt
      181 ctgctcaggt tcgaaactgc cagcggtcag aaaaccagag ctgggcagga taaattccag
      241 gaagcaataa aaaactgcct ggaatacctg cgagtggaat cagagcgtct gagcatggcc
      301 tttcgaaatt acgataatct tgcggccgtg gccgaactga aaacgaaaca gaaatcgcag
      361 cgctcggtgg gtgtggccgc tcatattgat gtcgtccccg ccggcaaggg ctggcgttat
      421 cccccatttg gaggaactgt tgccgagggc tgtatctggg gacggggtgc gcaggatgat
      481 aaaggtcctg tagcatctgt ctattctgca ctggacgcct tgaaaagtct gggcctgagg
      541 caggtcagcg atattcgatt tctgatcggc acattggaag agactgacga ctggcccgat
      601 gtcgatctcc tcagagataa aagggaaacc ccggatgtca ccattgtgcc ggacggaata
      661 tttcctgtca taaacgctga aaagggcatg gccatgctgg agctttccgg cacatggccg
      721 gaagagaaac catcagcgga aaaacctcga tttatttccc ttaccagcgg ccagcggcat
      781 aatatggtac ccgatactgc agtactgact ctgctggcgg aatcatctca aaaaagcgcc
      841 atcgctcgca gactgactga tactgctgtg acattgaaag agattattcc cgatagcgat
      901 ttttcaatga ctcataaaga agactccaag aattcgggaa agacaatctt tgaactgagt
      961 ttccttggta aaagcgcgca tggcgccttt cccgagaagg gtcataatgc cgctcttgat
     1021 gcgctggcat ttatcgatat gcttgaatac ggccctgagg gattgcgtgt ttttgcaggg
     1081 ggattgagcc ggcgctgcgg gctgctggat ggctccggat tcggtatcgg ctgtcatcac
     1141 gactatatgg ggaacaccac aatcaatctg ggggtgctca ctcttactcc aaaatcgggt
     1201 aatgcgcttg taaacgtgcg ttacccgtcg ggagtggaaa gcgctaatgt gcgggcggct
     1261 ttcgaaaaag cggccgccga agactcaaat gctttggacg gcctgaaagt caaatcccgc
     1321 attcatggcc gcatccatga ggccctgttc atctctccgg acaatcatcc cgagtttttg
     1381 cactctcttc agattgccta tgaaactgtt accggccgtc cagcgacctt gaattccatt
     1441 cggggaacca cctatgccaa ggcttttccc gcagcagttg cctttggccc gctggatgaa
     1501 aaggctggtg atatagagat ggcgcatgag gtcgatgagc gcatcaggat tgaccgttat
     1561 ctggaaaata tcaaaatata tgccctggct ctggctttgc ttgcatataa tcaccaacat
     1621 tattaatatg ggttggaaac aggagtaatt tttggataat gagagttaca tttactttta
     1681 atttgcgcaa gtccgaggac gatgagaaac aggccgagcg tttcagccag gagtatgtgg
     1741 acagagtcgt ctcagccctt gaagagctcg gacaccatgt atctccggtg gaggtttccg
     1801 gatctccggg agaggtggtc gataagctga tagattcgcg tcccgacctg attttcaacc
     1861 tggccgaggg ggtgggaggc aa
//
LOCUS       DSEB01000449            2622 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1005553, whole genome shotgun sequence.
ACCESSION   DSEB01000449 DSEB01000000
VERSION     DSEB01000449.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1005553"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1470
                     /locus_tag="ENO07_08020"
     CDS             <1..1470
                     /locus_tag="ENO07_08020"
                     /inference="COORDINATES: protein motif:HMM:PF13360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01953.1"
                     /translation="DIDGLVKITAKLEAFSLPGCERLWFNEYPIGSQADITGIKDLPY
                     HLWADEDAGLLFVGNGPLNAVDCKTGELLWSVDYDQTGMVYDVTPCGDYLFAMGTKTR
                     SKFTITFSQDYGSALDHIKDPILYCLDKKDGTIKWKYEYDPPRRATKKKHRGYYIEEK
                     ISQPFPGLYLPEGMLQENSNVEQGGIYILGKYVYCLDPSDGSEVWVYKNEPSGEIAFS
                     DKYLYVVADNKLCCLDRASGREVWKSDKKIKGRPFLAELADYDDHVIYACIDGKMTAI
                     DFEHGEQIWRCDKKEIDYLGDIVLRGNFLLAIDPGGYDEDKGEHTGKYRVVCHDLSSG
                     EMLWKYDDAENFRDWNMYNDTLLQVMDKDRIRIFNLNIRKKIGEIKVKDYYNFYPLEA
                     QFIDAGEKGVICYGFDGKTKIWEVKARLSKRDYSEFIEDLNLVVFPTRDEGVLGISTA
                     DGSVVWKTIEIKDPVLYYRPSYEYFLALDKKTLHRFDLQ"
     gene            1595..>2622
                     /locus_tag="ENO07_08025"
     CDS             1595..>2622
                     /locus_tag="ENO07_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01954.1"
                     /translation="MKKVFMLTIMLTITFMATDAILAGNSLGLLVSNASSLDIEEQAA
                     YDFAASHGFTVTIIDPSIISGDPSILNSVDGFWAANNAAPAGFNQPVVSDALRAQLEG
                     GKRMLVSWYGVYLVSYMGIASVNMSSSWYPSVSDHMYWVDKRIDHPVFENVPEWIPPT
                     MPDNASQLLAYVTPGYAPGGPYISGGPATILQYAQLWVTYGWCGQSFNPELQTEYGIT
                     GTCERAVRVDSFAEYPVGSGSVFAKFWSLTGGNNFHYGALGYQLLENALNYVCSGFGP
                     RNILLYTTRNMYGNGILRTDFDDDLPAILATEGFTVTAEDRGSMPELTSEILSEYSQL
                     WFVSGQKQT"
BASE COUNT          701 a          581 c          659 g          681 t
ORIGIN      
        1 gatattgacg gcctggtgaa gattaccgct aagctggagg ccttcagtct gccgggctgc
       61 gagaggctat ggtttaatga atatcccatc ggcagccagg ccgatatcac cggaatcaag
      121 gatttgcctt atcatctctg ggctgatgaa gatgccggtc tgctgtttgt cggcaacggt
      181 ccgctgaacg cggtagactg caagaccgga gaattgctct ggtcggtaga ttatgatcaa
      241 accgggatgg tgtacgatgt cactccctgc ggcgattatc tctttgccat gggaactaaa
      301 acccgatcaa aattcaccat aacattttca caggattatg gctccgccct ggaccatata
      361 aaagacccaa tcctgtactg ccttgacaaa aaagacggca caataaaatg gaaatatgaa
      421 tatgacccgc ccaggagagc aacgaaaaaa aagcaccggg gatattatat tgaagaaaaa
      481 atctcgcagc cgtttcccgg tttatatctg ccagaaggaa tgctccagga aaatagcaat
      541 gtcgagcagg ggggcatata cattctcggc aaatatgttt actgccttga tccatccgat
      601 ggctccgaag tctgggttta taaaaatgag ccctcggggg agatcgcttt ttctgacaaa
      661 tacctttatg tcgttgccga caataaactc tgctgtttgg atcgtgccag cgggcgggaa
      721 gtatggaaaa gcgataagaa gatcaaggga aggccctttt tggctgaatt ggcagactac
      781 gatgaccacg tcatttacgc ttgcatcgat ggaaaaatga ccgccattga ttttgagcac
      841 ggtgagcaaa tatggcgctg cgacaagaaa gaaattgact atctgggaga tatcgtatta
      901 agaggaaatt ttctcctggc tattgatccc ggcggctacg atgaagacaa gggtgagcat
      961 acgggcaagt atagagtggt ctgccatgac ctctccagcg gtgaaatgct gtggaaatat
     1021 gatgatgcgg aaaatttccg cgactggaat atgtataacg atacgctgct gcaggttatg
     1081 gataaggatc gaatcaggat attcaatctc aatatccgga agaagatcgg ggaaatcaaa
     1141 gttaaagact attataactt ttatcccctc gaggcccaat ttattgatgc gggtgaaaag
     1201 ggtgtgatct gctatggttt tgacggaaag accaaaatct gggaggtcaa ggccaggctt
     1261 tcaaaaagag actattccga gttcatagaa gacctgaatc ttgtagtttt tccaaccagg
     1321 gatgagggag tgctgggtat ctctaccgct gacggttcgg tcgtctggaa aaccatcgag
     1381 atcaaggatc cagttttgta ctatcgtccc tcctatgaat attttctggc tcttgacaag
     1441 aaaacactgc accgttttga tctgcagtaa ataagataaa taatgatttt tgagcaagaa
     1501 gcggactggt tggtctgctt actttgaaac tggagagaca aaatactgca aatatcaata
     1561 cttcaaactt caccctaatc ccagggagga ttacatgaaa aaagttttca tgctcacaat
     1621 tatgttgacc attaccttta tggcaacgga cgcaattctg gcaggcaatt cactcggatt
     1681 gcttgtatcc aatgcctcat ctcttgatat cgaagaacag gcggcctacg attttgcggc
     1741 cagccatggt tttacagtta caatcattga tccctcaatt atttcaggcg atccgtctat
     1801 cctgaatagt gttgacggtt tctgggcggc aaacaatgct gctccggcag gatttaatca
     1861 accagtggtt tcggatgccc tcagggctca gctggaagga ggcaaacgta tgcttgtctc
     1921 ctggtacggt gtgtacctgg tttcatatat ggggatagca tctgtcaaca tgtcatcaag
     1981 ctggtatcct tcagtaagtg accacatgta ctgggttgac aagcgtatcg accacccggt
     2041 ttttgagaat gtgccggaat ggattccacc gacgatgcct gataatgcct cgcaactcct
     2101 ggcatacgtt accccgggct atgcccctgg agggccatat atatcgggtg gccccgcaac
     2161 cattctgcaa tatgctcagc tatgggttac ttatggatgg tgcgggcaga gcttcaatcc
     2221 tgagcttcag acggaatacg ggataacggg cacctgtgag cgtgctgttc gggtcgattc
     2281 ttttgccgag tatccggtcg gttcagggag cgtattcgct aaattctggt ctctcaccgg
     2341 cggcaacaac ttccattacg gcgcgctggg ctaccagctc ctggaaaatg ccctgaacta
     2401 tgtctgctca ggttttgggc cgcgtaacat cctgttatat accactcgca atatgtatgg
     2461 taatggaatc ttgagaaccg actttgatga tgatctgcca gcgatacttg caaccgaagg
     2521 ctttactgtt actgctgagg accggggcag catgccggaa ctgacctcag aaattctttc
     2581 cgaatattct cagctgtggt ttgtttctgg ccagaaacaa ac
//
LOCUS       DSEB01000450            2199 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1008211, whole genome shotgun sequence.
ACCESSION   DSEB01000450 DSEB01000000
VERSION     DSEB01000450.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1008211"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            421..1386
                     /locus_tag="ENO07_08030"
     CDS             421..1386
                     /locus_tag="ENO07_08030"
                     /inference="COORDINATES: protein motif:HMM:PF04015.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDS01955.1"
                     /translation="MGEKMDRRKFIIQTGKASAAAAVFAGGYFALDLKPDFPEEKQAR
                     AVSADYRIPDRALDSKLAVAEGDDVRELGRKVVDSLGGMEKFISRDDRVVIKPNVAWD
                     RTPEQAATTNPELVAELVSMAYAAGASKVIVTDVTCHDPRRTFARSGIQEAAQKAGAE
                     VIIPGEQDFVEVDIRGELLTAWPVLKYFIETDKVINVPIVKAHSLSRMTIGLKNLYGI
                     IGGTRGQLHQKIHRSIVDLATFLRPTLVVVDAYRVLMRNGPVGGSLDDVKSARTVYAT
                     TDQVAADAFGCQFLDLDPSDVGHITLAEREGLGTADYKSKEIIRS"
     gene            1166..>2199
                     /locus_tag="ENO07_08035"
     CDS             1166..>2199
                     /locus_tag="ENO07_08035"
                     /inference="COORDINATES: protein motif:HMM:PF12801.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDS01956.1"
                     /translation="MHIVCLCAMVRWGDHWMMSRVRALFMPPPIKLPRMHSAASSWIS
                     IPPMSAISLSPNVRGWARRTINQRRLSEAEMTIQHVRNIRRAVQAVVLILFLVLLLKT
                     EFAGTFSADALRDYRLAYPVKIFLQFDPLASFMTAVSTLTIFSGLLFSLIIIVLTIFF
                     GRFFCGWVCPMGTINQLCSTFKSERRSRRGMNLVKSNQYHWYQKIKYYILIFFIGSAV
                     LGLMIAGIFDPISLLIRSLGLVVLPILSGLLDFVADFSFDNNFPGNAILGFVSGWIQE
                     NVLVTHSVVSYNTIFSLGLFFLLILAANRWFTRFWCRGLCPLGAFLGLISRWSIFGME
                     KYESECDHCN"
BASE COUNT          489 a          541 c          581 g          588 t
ORIGIN      
        1 cccatagatt attcaaaata tcctccaaat ttatgggctt taatctataa ttgctttatg
       61 gtattgtcaa gcatacaaat tatacgaaga taatcttgat ttgccaaagt ataaatccgg
      121 gataagtgtt cacagatatg cgccagggtg tcctgtcccg gatgggtctc ttgaaaaagt
      181 gtgtatggat tgaaactgtc attctgagcg cagcgaagaa tctttttgct tcgtgagaga
      241 tttttcggtc accacggtcc ctcagatgac ggcggatact tgtattctcc atattccgtg
      301 ctttctcaac atttccctga gctggggggg caggcaaaca tccaaatccc tccaatagca
      361 aatttaatat tgccgggctc ttctatcctg cgtatcatta taaaaaccag aggacagctt
      421 atgggcgaaa agatggacag acgtaaattt atcattcaga ccggcaaagc ctcggccgcc
      481 gcagcggtat tcgcgggagg atatttcgcg cttgacctca aacctgattt tccggaggaa
      541 aaacaggctc gagctgtgag cgccgattac aggattccgg atcgcgctct tgacagcaaa
      601 ctggctgtgg ccgaagggga tgatgtgcgt gagctggggc gtaaagtggt cgattctctg
      661 ggcgggatgg agaagtttat ttcccgggat gaccgggtgg ttatcaaacc caatgtggcc
      721 tgggaccgta ccccggagca ggccgccacc accaatccgg agcttgttgc ggaactggtc
      781 agtatggcct acgctgcggg agcctccaag gttatcgtta ccgacgtcac ctgtcacgat
      841 ccccgccgga ccttcgcccg ttccggaatc caggaggcgg cgcagaaagc cggggcagag
      901 gtgataattc cgggtgagca ggattttgtc gaggtcgata tcaggggcga gctgctgact
      961 gcctggccgg tcttgaaata ttttatcgaa acagataaag tcataaatgt cccgatcgtg
     1021 aaggcgcatt cgctttcccg catgacgatc gggctgaaaa acctgtacgg cataatcggc
     1081 ggcacacgcg gacagctgca tcagaaaatc catcgttcca tcgtcgacct ggcaacattc
     1141 cttcgcccta cactggtagt tgtcgatgca tatcgtgtgc ttatgcgcaa tggtccggtg
     1201 gggggatcac tggatgatgt caagagtgcg cgcactgttt atgccaccac cgatcaagtt
     1261 gccgcggatg cattcggctg ccagttcctg gatctcgatc cctccgatgt cggccatatc
     1321 actctcgccg aacgtgaggg gctgggcacg gcggactata aatcaaagga gattatcaga
     1381 agctgagatg acgatacagc atgtcagaaa tatccgcaga gcggtgcagg cggtggtgtt
     1441 aatccttttc ctggtgctgc ttcttaaaac cgaatttgcc gggacctttt ccgccgatgc
     1501 gctcagggac tatcgtctgg cctacccggt taagatattt ctgcaattcg atccgctggc
     1561 atcatttatg accgctgtct cgaccctgac gattttctcc ggactgctgt tttcgctgat
     1621 aattattgtt ttgaccatat tttttggacg tttcttctgc ggctgggttt gtcccatggg
     1681 tacgatcaat cagctctgct cgacctttaa gtccgaacgc cggtcgcgcc ggggaatgaa
     1741 tctggttaaa tcgaaccagt accactggta tcagaagatc aaatactata tcctgatttt
     1801 cttcatcggt tcggctgtac tgggtctgat gatagcagga atattcgatc cgatttcgct
     1861 tcttatcaga tcattggggc tggtagttct gccgatcctc tccggccttc tcgattttgt
     1921 ggccgatttc tcatttgata ataacttccc cggcaatgcg atcctgggct tcgtctcggg
     1981 atggattcag gagaatgtgc tggttacaca ttcggtggtc agctacaaca cgatcttctc
     2041 actcgggcta ttcttcctgt tgatcctggc tgccaaccgc tggttcacac gattctggtg
     2101 tcgcgggctt tgcccgctgg gagcatttct ggggctgatc tcgcgctggt cgatcttcgg
     2161 aatggagaaa tatgaatcgg aatgcgatca ttgcaataa
//
LOCUS       DSEB01000451            2717 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1005120, whole genome shotgun sequence.
ACCESSION   DSEB01000451 DSEB01000000
VERSION     DSEB01000451.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1005120"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            902..2278
                     /locus_tag="ENO07_08040"
     CDS             902..2278
                     /locus_tag="ENO07_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018842824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDS01957.1"
                     /translation="MENFGANLKGELIRSGDASYDECRAIWNGMIDRRPALIVRCGNQ
                     DDVIAAVKFARQYDLITSIRSGGHHVAGKAVCDSGLVIDLSLMNKVEVDPKERIARVQ
                     GGAKLGDLDEATSQYNLAAPVGVVSETGVAGLTLHGGFGWHSRKYGLAADNLLSVDVI
                     TADGRHLRASKDENQDLFWALRGGGGNFGVVTSFEFKLHPIESEVWLLMTLYPLSKAR
                     ECMEYFREHMPNTPEEFSMINVFWSAPDQEAIPEQYRGLPVLVSFGCCLGDPDKAEKT
                     IAPFRQISEPIADLSARMPFKEVQKVLDEDYPNGRHYYWKSVYINEISDDTIDRLIEY
                     AENRPSALSSIDVWGLGGQVNRIKPESTAFYQRNAPYMIGIEANWDDPDDSQRNIKWA
                     RAIYDEFKNNLDAGLYLNFPGFAEEGEELLAKTYGPNYQKLKDIKAKYDPDNFFRGFM
                     NLSGNGSR"
     gene            complement(2457..2684)
                     /locus_tag="ENO07_08045"
     CDS             complement(2457..2684)
                     /locus_tag="ENO07_08045"
                     /inference="COORDINATES: protein motif:HMM:PF00403.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01958.1"
                     /translation="MMKHLRISGMTGAYSADTIKNSLEMVSEVDSAYVNYEKNSAQVN
                     FSGLEPSDEVLINAVKDAGYTAEVIKEERKK"
BASE COUNT          649 a          640 c          718 g          710 t
ORIGIN      
        1 cccgccctac gcaccggctt cgcccgatcc ggctgacggc gcgacaggag ttctgctcag
       61 cgcttcattg agctgggtgg gcggcgaccc caacaccaat gaccaggtgc attatgatgt
      121 ctattttggg acagccgatc cgccgcctct ggtgagcgac gaccagcttg cgccggccta
      181 cgatccgccc ggtgatctgg aatacggcac gcagtatttc tggcagatca tagccttcga
      241 tgaggtggaa gaatcgacgg ccgggccgat ctggacattt acgaccgagt atgatgtgct
      301 ctgcggcgac tgcaatgatg acttgttggt gaatgtttcc gatgcggttg tgatcattaa
      361 ttttgtattt ttgggcggca caccgcctga tccattgtgt gagggggatg ccaatgcgga
      421 tggttcggtg aatgtgtccg atgcggtagc gataattaat tttgttttcg tcggcggcga
      481 tccgcccagt ccaaattgtt gcgggaaata gaattaacca tagatgatta ctatgatcgg
      541 gcaggtttct tgtgaacctg cccattttgt tatactttcc ttgtgtgcat cccgattttt
      601 ggccgggtcg cagtaacaaa tcaggaaagt gtaaatgctc ccctgacaca tcggcgtacc
      661 tcgaaaagcg cgttttctga accgaaagat aatgttgtag atcgattaat ccattgtggt
      721 ctaatcaagt acaataattt ttgcagaacc caaaaataat cgaaacaaaa tcaagataag
      781 ttttgtattg ttttatgtgt gatctgttca tttttgtact ttattcaaga aggggattat
      841 tatggatgct ttcaatgctt tgacattgac aggagagaaa actaccctgt ctccatcgga
      901 tatcgaaaac ttcggggcaa atttgaaagg ggagttgatc cggtccggtg acgccagcta
      961 tgatgagtgt cgggccatct ggaacggcat gattgaccgc cgacccgcat tgatagtcag
     1021 atgcgggaat caggatgatg ttatcgccgc ggtcaaattc gcacgtcagt atgatctgat
     1081 taccagtatc cgttcaggcg ggcatcatgt agccggcaag gcagtttgcg acagcggact
     1141 tgtaatcgat ctcagcctga tgaacaaggt tgaggtcgat ccgaaagaga gaattgcccg
     1201 tgtgcagggc ggggcgaagc ttggagacct ggacgaggcc accagtcagt ataatcttgc
     1261 ggctccggtt ggcgtggttt cggagacggg agtagccggg ttgaccctgc atggcggatt
     1321 cggatggcac tcgcgcaagt acgggcttgc ggctgataat cttctatctg tagatgttat
     1381 cacagcggat ggcagacatt taagggccag caaagatgaa aaccaggatt tattctgggc
     1441 cctgcgcggc ggaggcggca atttcggggt ggtcacctca ttcgaattca agctccatcc
     1501 aatcgaatcc gaggtctggc ttttaatgac gctttatccg ctctcaaaag cacgcgaatg
     1561 catggaatat ttccgcgagc acatgcccaa cacacctgag gaattcagca tgattaatgt
     1621 tttctggagc gctcctgacc aggaagctat tcccgagcag tatcgcggcc ttccggtttt
     1681 agtctccttt ggatgttgcc ttggcgatcc ggataaagcc gagaagacaa ttgcgccatt
     1741 caggcaaatt tccgagccca ttgccgatct cagcgccagg atgccgttca aggaggttca
     1801 aaaggtactg gatgaggatt atcccaatgg tcggcactat tactggaagt cggtatacat
     1861 caacgagatc agcgatgata ccatcgacag acttatagag tatgccgaaa atcgtccttc
     1921 cgcattgtca tcaattgatg tctggggtct gggagggcag gtaaatcgca tcaaaccgga
     1981 gagtacggca ttctaccaga gaaatgcgcc atatatgatc ggcatagagg caaattggga
     2041 tgatcccgat gactcccagc gcaacatcaa atgggcgcga gctatttatg acgaattcaa
     2101 gaataatctt gatgccggtc tctatttgaa cttccccggc tttgccgagg agggtgagga
     2161 attgctggcc aaaacctatg gccccaatta tcagaaatta aaagatatca aggccaaata
     2221 tgatccggac aattttttcc gcggttttat gaatctttcc ggtaacggca gccgttaaaa
     2281 ttagggtcag gggcggcggg ttagctgaga tcgctcgata attctttctt tctgcttttt
     2341 gccagttcgc ccccggcaac ggcgggacag gaactgaaag aattcgatag aaagttatac
     2401 cattgtgcgg gctcaatagt ctgccgcctg atacctggtc tgctgcccca ctccgtttac
     2461 ttcttgcgtt cttctttgat gacttcggca gtataaccgg catccttgac ggcattgatc
     2521 agcacctcat ccgacggttc caacccgctg aagttcacct gggccgaatt tttctcgtaa
     2581 tttacatagg ccgaatcgac ctcggaaacc atctccaggg aattcttgat tgtatcggcg
     2641 ctataggcgc cggtcatgcc ggatattctc aaatgtttca tcatggtatc gccctccttt
     2701 cagctatctt gttgatc
//
LOCUS       DSEB01000452            2358 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1007022, whole genome shotgun sequence.
ACCESSION   DSEB01000452 DSEB01000000
VERSION     DSEB01000452.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1007022"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            449..2113
                     /locus_tag="ENO07_08050"
     CDS             449..2113
                     /locus_tag="ENO07_08050"
                     /inference="COORDINATES: protein motif:HMM:PF05833.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding domain-containing protein"
                     /protein_id="HDS01959.1"
                     /translation="MKITALHTARLVSEFRQSLPGARIRKLRKSERDKTIVMHLEDAH
                     DRYSLIFHFGGKDSYLYLLDGFYTRITTTNFLPQLLDARIINICQIDFDRVVQFDIED
                     DERSFKLIFELFGISSNLFLTDGSGTIITSIRKAKLDLKSYRAPEPLKLANPLEFDVE
                     NIMGMIGSNSELSLREAILKTFAGIDEGMLRDFEEEGSNVLDEAVSKIRPETIRNILC
                     NLRKYCADFIKPETELSHDPESESILLSSDGDLQQYSSISDIFRQYAAGQTLRKPKST
                     IKSSLAGQLKRAIKKDTKKQAKLEEQLGKAEEYPDLQRRAELLAMNLHKAGRGMDSIE
                     LEDVYSDRGGVIRVELEKSLTPGQNVERLFARAKKLKEKIPGIREEIQFTKDRLERLE
                     KLRSELDRLASDELPQELERELAEFGIRPPGRGRKAEPRERLPYKRYTSSSGEEIWVG
                     RSAANNDILTFKYARKYDLWLHSQQTSGSHVILRRPNKNHQFQKSSIVEAAEIAAFFS
                     TAKKSDSVPVIYTEVKYVRKLRKGRPGEVLADRTKSILVTPRRPKN"
     gene            2153..>2358
                     /locus_tag="ENO07_08055"
     CDS             2153..>2358
                     /locus_tag="ENO07_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01960.1"
                     /translation="MKTVAFIIIILAVAAGLYFLLKPEKDIMSDPRIDKFARIQAEIE
                     IEAVRNNQDSVLMDSVRDSIYEYY"
BASE COUNT          675 a          523 c          588 g          572 t
ORIGIN      
        1 caattgttgt tgatccacaa tctgcggatt ctgagactca gcccaggcgc ctttctgata
       61 gacgatcaaa ttgagccaat tttgaccaac ctgatccatc tcataatcgg atgcaacctc
      121 ggccggggcg cttgaaaaaa gaaataggat tgcgaaaata gaaatagcga ttgcctggaa
      181 aatagaagtc attacttttc ctggcgatga ggatagctta ctattgtaga attgtctttt
      241 atgggaatac aatgcgccac ctcccgggcg ataggccagt agttaatgaa gaagctgttt
      301 tcattattaa aagataaccg gcttcgggtt tttgtcaata tttgaattgt cgttctcttt
      361 tatgtgctta aaactcttgc aattatgacg gcaatactgt aacctttatt tgtttatatt
      421 tcggaagttc attggtaaat tggagcgtat gaagatcacg gccttacata ctgccagact
      481 ggtgtccgag ttcaggcaat cccttccggg ggcgagaata agaaagctca gaaagtcgga
      541 aagggataaa acaatagtca tgcatttgga agatgcgcat gacagataca gcctgatatt
      601 tcatttcggc ggcaaggatt cctatctgta tctcctggat ggcttctata cccgcattac
      661 caccaccaat tttctgcccc agcttctgga cgcaaggatc attaatatct gccagattga
      721 tttcgatcgg gtagttcaat tcgacataga ggatgatgag cgcagcttta agctgatatt
      781 cgaattattc ggtatcagca gtaatctgtt cctgactgat ggttccggga ctatcataac
      841 ctccattcga aaggccaagc tggatctcaa gagttatagg gcgcctgaac cgttaaaact
      901 ggccaacccg cttgaatttg atgtagaaaa catcatggga atgatcggct ccaacagtga
      961 gttaagtttg agggaggcaa tcctgaagac attcgcaggt atcgacgagg gaatgcttcg
     1021 agattttgaa gaagaggggt ccaatgtcct ggacgaggca gtttccaaaa tccggcctga
     1081 aacaatccgg aacattttat gcaacctcag aaaatattgc gcggatttca ttaagccaga
     1141 aaccgagctc agccacgatc cggagtcgga atcgatattg ctttcaagtg atggcgattt
     1201 gcagcaatat tcctcgatat cggatatatt tcgtcaatat gcggccggac agacgctcag
     1261 aaaaccgaag tccacaataa aatcaagcct cgccggccaa ttaaaaaggg ccatcaaaaa
     1321 agatacaaag aagcaagcga aactggagga gcagcttggg aaagcggaag aataccctga
     1381 ccttcagcgc cgggccgagc ttctggccat gaaccttcat aaggccgggc gggggatgga
     1441 ctcgatcgag cttgaggatg tctattctga tcggggcgga gtaataagag tcgaactcga
     1501 gaaatcattg actcccggcc agaatgtgga acggctcttt gcgcgggcca agaagctgaa
     1561 agagaaaatc cccgggattc gagaggaaat tcagttcacc aaagaccggc tcgagcgtct
     1621 tgaaaaactc agatccgaac tggatcggct tgcctccgat gagcttccgc aggaactcga
     1681 gagggagttg gccgaatttg gaatcaggcc gccggggcga ggcagaaaag cagaaccccg
     1741 ggagcgtctt ccatacaaga ggtacacctc ttcatcggga gaggaaatct gggtgggccg
     1801 atcggctgcg aataatgata tactgacatt caagtatgcc cgcaaatatg atctctggct
     1861 gcattctcag cagacctccg gcagccatgt catcctgagg cggccgaata agaaccatca
     1921 atttcaaaag tccagtatag ttgaagcggc tgagatagcg gcattttttt cgaccgcaaa
     1981 aaaatccgac agtgtgccgg tgatctacac cgaggtcaag tatgtgcgca agctcaggaa
     2041 aggcaggccc ggggaggttt tggccgatcg gaccaaatcg atcctggtca caccccgcag
     2101 accaaaaaat taagctttta ttttcctcaa agcttttata aattcactcc cgatgaagac
     2161 agttgcgttt ataataataa ttcttgcggt tgcggcgggg ctgtactttc tcctgaaacc
     2221 ggaaaaggac atcatgtccg atccccggat cgataagttt gccagaattc aagctgaaat
     2281 tgaaattgaa gccgtgcgta acaatcagga cagcgttttg atggacagcg tccgcgactc
     2341 gatctatgag tattataa
//
LOCUS       DSEB01000453            2297 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1007443, whole genome shotgun sequence.
ACCESSION   DSEB01000453 DSEB01000000
VERSION     DSEB01000453.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1007443"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..983)
                     /locus_tag="ENO07_08060"
     CDS             complement(<1..983)
                     /locus_tag="ENO07_08060"
                     /inference="COORDINATES: protein motif:HMM:PF01041.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDS01961.1"
                     /translation="MAGKSKASMCVHAGEEKMEFAGAVVPPIFNTSTYVFKNMQELRD
                     YVKGDSPYYLYSRYTNPTVEAAETKLAALEGGEKCLIVSSGQAAAGTAVMGILRDGDH
                     MVATPTLYGGVFSLFKDIINKTGLQVEFADSTSVADVKKAIRPNTKIIYTETPTNPNL
                     TVVDLEGIAKTAREHDAIMIVDNTFATPMNQNPLELGADIVVHSGTKYLGGHSDLVCG
                     AIIGPHKYLRTIYEYRKNMGTNLDPHAAFMLLRGMKTLAIRVERSCENAMKVAEFLEQ
                     HPKVRKVRYPGLPSFPYHEMAKKQMRRFGGMVCFEIDGGLDAVGKVIEAMH"
     gene            complement(985..1770)
                     /locus_tag="ENO07_08065"
                     /pseudo
     CDS             complement(985..1770)
                     /locus_tag="ENO07_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_430344.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAA hydrolase family protein"
     gene            complement(1771..2297)
                     /locus_tag="ENO07_08070"
                     /pseudo
     CDS             complement(1771..2297)
                     /locus_tag="ENO07_08070"
                     /inference="COORDINATES: protein motif:HMM:PF00850.17"
                     /note="incomplete; partial in the middle of a contig;
                     missing start and stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="histone deacetylase"
BASE COUNT          532 a          666 c          508 g          591 t
ORIGIN      
        1 atatgcatcg cctcgatcac cttgccgacc gcatccagac ccccgtcaat ctcaaaacag
       61 accatccccc cgaagcgacg catctgcttt tttgccattt catgatatgg gaatgaaggc
      121 aatcccggat atctcacctt gcgaactttc gggtgttgtt ccagaaactc cgccactttc
      181 atggcgttct cgcatgatct ctccacccgg atagcaaggg ttttcatacc cctcaaaagc
      241 ataaatgcgg catgcggatc gagattggta cccatatttt tgcggtattc atagattgtc
      301 ctgagatatt tgtgcggccc gatgatagcc ccgcagacca ggtcggaatg accgcccaga
      361 tattttgtgc cggaatgaac gactatgtct gcgcccagtt ccagcggatt ctggttcatc
      421 ggtgtggcaa aagtattatc aacaatcata attgcatcat gttctctggc cgttttcgcg
      481 atgccctcca gatcaaccac cgtcaggttg ggattggttg gtgtttccgt atagatgatc
      541 ttcgtgttag gcctgatagc tttcttcaca tctgcaactg aggtcgagtc tgcaaattcc
      601 acctgcagtc cggtcttgtt tatgatgtct ttgaagaggc tgaagactcc gccataaagc
      661 gtgggggtgg ccaccatatg atcaccgtca cgcagtattc ccatcaccgc ggttccggcc
      721 gccgcctgcc cggacgatac aatcaggcac ttctcccccc cttcgagcgc agccagcttc
      781 gtctccgccg cttccaccgt ggggtttgtg tagcgcgaat aaagataata cgggctgtcg
      841 cctttaacat agtcgcgcag ctcctgcatg ttcttgaata cataagtcga ggtgttaaaa
      901 atcggcggga ccacggctcc cgcaaattcc atcttttctt ctcctgcatg tacgcacatg
      961 gaggcttttg attttcctgc catcttactc tcctatatag ttaatcaatc tgccaacatt
     1021 ctctacaacc acttcaattt tatctcccac tttcatggga tcgatcccct ccggggtgcc
     1081 ggttgaaatc aggtctcccg gttttaaggt cataactttg gaaatgaacg atatcagcac
     1141 ctccggtttg aaaatcatat ccgaggtcct tcccgattgt ttcagctcgc cattcaaata
     1201 agcttctacc ttcagatccg accagtctgt atctgtaatc acccagggtc ccacgggaca
     1261 gaaggtgtcg aagcctttgg cacgggtcca ttggccatcc ttcttctgca gatgccttgc
     1321 ggtaacatca ttcacacagg tcaatcccca gacagctcca gcggcctcat cgggcgtgac
     1381 gttcctgatt ttctttccga tcaccacccc gagctcggct tcatagtcca cacgatccac
     1441 aaaatcagga tagatcacag tcccttcatg acccagaaga gagctgggag gcttcatgaa
     1501 caggagcggc tcctctggag ccttatccgc cgactgggaa tgactgacgt gcttatgata
     1561 attcagcccc acgcagatta tcttctccgg ctgcaccggc gcaagcagtc tcacatcctc
     1621 aaggtcaaac ttcttttcgc tcactgcata gctgtcaaaa agacttcctt cgataacttt
     1681 aatgctcagc tcgctttcgt cataaatacc ccagaagatt tcagaatccg attcaaaacg
     1741 aacgatctta tccgacataa actactccat taatatcttt aagtgctcaa ctactgccct
     1801 gccaatgtaa tcaaggacat atccgccctc cagcacgaga actatcctgc cgtcacaata
     1861 ttcttccgca gctgcctttg cgatccggca gatttcgctg atagcctccg gggagacatc
     1921 caggaaaaca taagggtcgg cggtgaatgc atcatagccg gccgagacca aaattatttc
     1981 cggccggtag tcactcatgg ccgggagcca ttcttcctta acgggcccca gaaactgctc
     2041 gtcgccggca cccaggccca gcggaaaatt aagagtgtat cccttcccgc tgccggtacc
     2101 ggtttcattt tttgtgccgc tcccgggata aaagggaaag gcatgcaggc tggcatagtg
     2161 cacccgctcg gtatcataga atgcgtgctg ggtgccgtta ccgtgatgac catcccagtc
     2221 caggatggca attctctcca gattgtagtc agcccacagc cgccgcgcca atatggcaat
     2281 attgttaaat atgcaga
//
LOCUS       DSEB01000454            2864 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004551, whole genome shotgun sequence.
ACCESSION   DSEB01000454 DSEB01000000
VERSION     DSEB01000454.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004551"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            2356..>2864
                     /locus_tag="ENO07_08075"
     CDS             2356..>2864
                     /locus_tag="ENO07_08075"
                     /inference="COORDINATES: protein motif:HMM:PF00440.21"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDS01962.1"
                     /translation="MQDERKKIFDAAVEEFTEHGIKATSMKAISERAGLDEGAARALF
                     VDKERLFRELLKEYSEPLVSASGFVAQDIDDPRQFLRKSLKLYDRWLLENPRLVQLIA
                     WGIAEGVQFFGSIYSHVLYPSEFLEKLQKFIWDGQIRIKDIYTVFILIESMMVFSHLM
                     KSNIKSLYP"
BASE COUNT          734 a          605 c          729 g          796 t
ORIGIN      
        1 atacatatat atccctctgg tgcgaccctg atgtgggaga tgcaggagat gatctggttg
       61 gatgtgatac ggtattatcg ctcggttatt gctataatga gtcgggaggg gatgctattt
      121 atggtacagc agttccggca gtcggctttg acttcctgca gggaccgata atacccggag
      181 atcccacaga cagcgcaatt tttatgggtg aatggatata tggttacaaa aatatgccca
      241 tgacatcatt caataaatat atcaacggta ccgatccaca ttcacccatt gaatcttata
      301 attacatgaa aggcgactca ataagcggtg cgccattggt tgatccgttt ggtaacataa
      361 ccacatttat gcaccccggt gatcccgtag ccggtacggg atggctggat gtggctgctg
      421 atgatcggcg cttcatgatg agcaccggac cctttgacat gatgccggga gatacccagg
      481 aaatagtggc cgcaatagct gttgggcagg gatccaatag gcttgaaagt attactaatc
      541 tgaaagagca tgatcagatt attcagatgg tctatgataa cttcttcgat attcccagtg
      601 cgcccgttgg atttgaagct tatggccgag gtcttgatgg tgcgattgac ttggtctgga
      661 catccgacat ggaaaactac tatcaggatt atctcgatcc tcttgatcag ttctttgtct
      721 tcgaaggata taacatatat cagggcgaaa gcgagtccgg gccctggcac aaaatagcga
      781 cttttgatat ggatgcagct gagctgatgc agagctttta taatgtcgcg ggcgaatatg
      841 tcattgattg tacgggagag cctcctaatg aagaatgtga ttctgttcct cgtccctggg
      901 attttcaggc aatttacaat tgggtaataa atcctgtatc cggcctgccg gagctgagag
      961 tcgttcaggt gggcagcgaa tcaggaattg caaattacct atatctggat gaagatatgg
     1021 ttatgggcgg cccgataata aatgataatc catatcattt tgccgttaca tattacatgg
     1081 taaacataga ggatgttcgt ccggaagatt ctgtcttcgc cggtataaac ttcctcggct
     1141 tcaatgcggc tacgcttgaa actccgattg aggcattgac agtcattcct gagagcagtc
     1201 ccgtggtgga gtacacggac actgccgacc acatcgccgg taactcggat ggtattgtag
     1261 tgatcgaata tcttgacatg gaacagactg ttcccggcag ctatgaagta aatttcaatc
     1321 cggatggtac ttggaacctt gaacgcgact tcgtgacact acttttcaat cagagcaacc
     1381 aaagcggcgg ttatgattat gagatcgtgg acggtatcat ggtgcgtgtg atcggacccg
     1441 agccgggaat tgcgcctcca gccaatgatt atgggggagg tgttatcgag acttacagcg
     1501 cgtttggacc tgttgatcca ccggataatg ttttctggtc tatgaattca actggtgatt
     1561 ggtttatttc ctcggatatc ggtggcagca ccgatcaggc ccgggccaga ttcaactggc
     1621 gcggtttgat cggagttgaa tcatgggaaa tcaggtttac cgaaactgga agtgaatact
     1681 acgactggct tacagatgag aaatggccca acagggctcc tttcgaggtc tggcactacg
     1741 aaggtgatag cccaactcct gacagaagag acttcttctt tattattgat gatgatgctt
     1801 ccggcggctg gagctggggg gacagagtat atatagccga aactgaatat cccgccgaac
     1861 cgcttcccca gaatgccatg gatgccggct atgtctggcc agatgatttc catcttggca
     1921 gggttgtctt caatgatttt tccggctccc tgaatcatcc ggattatggg acgatcgcca
     1981 gatttaattc gactgtcccc aattctgtag aggatatatt cacgtttgaa gttcccgagg
     2041 tgccttattg cggtaatatt aatctcgata atgagattaa tgtagcggac gcaatatacc
     2101 tcctgaacta tattttcctg gagggtccgc agccttcaac gttcgagtct gcagatgtca
     2161 attgtgattc tatcataaat cttactgatg ctgtttatat catcgtctat atcttccagg
     2221 gggggaatac tccgtgcgat cccgatggcg atggttttcc cgaatgttaa ataaaaagaa
     2281 agtatttttt tcaagccccg cttagcgcgg ggcttttttt ctggctaatc atctttaaag
     2341 atttaaattg caggtatgca ggatgagaga aagaagatat tcgatgccgc tgtcgaggaa
     2401 tttacggaac acggaataaa ggcaacttcc atgaaggcga tctccgagcg tgcgggactg
     2461 gatgaaggtg cggcgcgggc cctgtttgtc gacaaggagc gcctcttccg ggaacttctt
     2521 aaagagtact cggaaccatt ggtctcagca agcggttttg tggcacagga tatcgatgac
     2581 ccgcgtcagt ttcttcgaaa atccttaaaa ctctatgatc gctggctcct cgaaaatcca
     2641 aggctggtgc agttgatagc ctggggaatt gccgaagggg tgcagttttt tggttcgatc
     2701 tacagtcatg tcctctatcc ctctgaattc ctcgagaaac ttcagaaatt catatgggac
     2761 ggacagataa gaatcaaaga catttatact gtttttatat tgatagaatc gatgatggtt
     2821 ttctcacatc taatgaagtc aaatatcaaa tcactgtacc cgga
//
LOCUS       DSEB01000455            4565 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001624, whole genome shotgun sequence.
ACCESSION   DSEB01000455 DSEB01000000
VERSION     DSEB01000455.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001624"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            1..1320
                     /locus_tag="ENO07_08080"
     CDS             1..1320
                     /locus_tag="ENO07_08080"
                     /inference="COORDINATES: protein motif:HMM:PF08668.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HDS01963.1"
                     /translation="MNRRPMSRIDILAKLDQADEIFSLPETLLEVLQEVESDDWSSKS
                     MAAIINRDSGLAAGVLRMANSSYYNRGSRISTVNRAVVVLGASMVKCLALSAAIFNPI
                     DEIKRKLNFDIRSLYTHYLSTAVAARQLAEKCGFARTEEAFTAGLMHDLGMMYIIRVA
                     PEEYVDLLGRYEAGEDLVKMERELFDTDHAEVGYLISRRWKLPAGIQNAIGNHHRLTS
                     YDNFGTHEKLDQIVALANLINRHIFSSSGCYVEENIQQVANLRRVLDLDQDAITEIND
                     KNLQETFRAAEQIGMEIGDPMTLLEKANRQIMRSYQTVESLFKERQELSRQIIEEGHK
                     LGAMESKNIAVATLSHYINNAASAISGRIQIVEMLLKNDMIVDKQGKLTPALEVIDTS
                     LKKILAVLAELKSLTSLDGVKFHNGSSTINIDENISQRIKTMKKVIC"
     gene            1517..3013
                     /locus_tag="ENO07_08085"
     CDS             1517..3013
                     /locus_tag="ENO07_08085"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:PRK00913.1-4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDS01964.1"
                     /translation="MKFNSQITKISQVSADCLAVPVYQSRFADNPHLKLFPKALKDHI
                     QALFKGDVITGREGDGYLLRVGGTHGFEYLYLAGIGKKEDLEHDSIRRFGGNLASSLK
                     GKKFKELAICVPESFQLDDDCSLVSQMAVEGLLLGSYELLKYKSEPSKKAMPARVSFI
                     LPDSWDKRRFNAGLKIGEIMAWGQNLSRDLIGHPSNYLTPVELANTARSMARTYGFKC
                     EVLNKARMKKLRMNCILAVNSGSKEPPQFIIMHYKGVGSKKDPVCLVGKGITFDSGGI
                     SLKKAQDMDQMKGDMGGAACVLASVAVAARLKLPVNLVGLIPATENLPSGSAYKPGDI
                     LTAMNGKTIEVLNTDAEGRLVLADALSYSQRLKPAAIIDVATLTGASMVALGYVSSAM
                     FTNDDKLRKLIMSGSQKSSEKTWEMPLWKEYQEQIKSSIADIKNTGGRPAGSCTAAAF
                     LNNFTADYPWAHLDIAAVDVVFEKGPYNPKGASGYGVRLISEFLREWK"
     gene            complement(3079..4068)
                     /locus_tag="ENO07_08090"
     CDS             complement(3079..4068)
                     /locus_tag="ENO07_08090"
                     /inference="COORDINATES: protein motif:HMM:PF07676.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01965.1"
                     /translation="MKRFSLLTLFFVFTLMTSIYLAGSSHAQGSESESMGEQIAFIRN
                     GDVWLMNPDGSDQRAFVVGISNAKGRMSWAPDNGRLAFSRQGKVDIKFPEGGGGYHYL
                     YDLFYAFPDSLPERKNFWMGFTNTLGSQSPDWSDNGKTICFTYDRMGNVVDASVPEYS
                     IGFYDPETETFSHMEMPQDEKQLFPLMPSISPDGSKVCFVLGELQSSGMKKLGLVVLP
                     LEGELPATDVLVETASNNPDGSSPDWSPDGTKILFLQSEGVYTANADLSGEKLVCKPE
                     EGLWITGMASWSPDGNKIAFGTSNGAIYTVNLDGSGMIRISGPGNDSNPTWTK"
BASE COUNT         1172 a         1109 c         1145 g         1139 t
ORIGIN      
        1 atgaatagaa gacccatgtc caggattgat attttagcta aattagacca ggcggatgag
       61 atattttcat tgccggaaac actgctggaa gttctccagg aagttgaaag tgatgattgg
      121 tcgtccaaat ccatggccgc cataataaac agagatagcg gtctggcggc aggagtactg
      181 agaatggcca attccagtta ctacaacagg ggaagtcgga tttccactgt caaccgcgct
      241 gtggtcgtac tgggtgcgtc gatggtcaaa tgcctggcgc tctcggcggc aattttcaat
      301 ccgattgacg agatcaaaag gaaactgaat tttgatataa ggtcactcta tacacattat
      361 ttgagcaccg ctgtcgccgc cagacagctg gctgagaagt gcggttttgc caggaccgaa
      421 gaggccttta ccgcgggact gatgcatgat ctcggtatga tgtatataat tagagtggcg
      481 cccgaggaat atgtcgatct tttggggaga tatgaagccg gagaagatct ggtcaagatg
      541 gagcgcgaat tattcgacac cgatcatgct gaagtcggat atcttatcag ccgcagatgg
      601 aaacttcccg ccggaattca gaacgccatt ggaaatcacc atcgcctgac aagctatgac
      661 aatttcggca ctcacgaaaa actggatcag attgtggctc ttgccaatct gattaacagg
      721 catatttttt cttcttcggg ctgttatgtc gaggaaaata tccagcaagt cgcgaacctg
      781 cgccgggtcc ttgatctcga ccaggatgcc atcacggaaa tcaatgataa gaacctgcag
      841 gaaaccttca gggccgccga gcagatcggc atggaaattg gcgacccgat gacgcttctg
      901 gagaaagcca atcgccagat tatgagatca tatcaaactg tcgagtcact tttcaaggag
      961 cgccaggaac tttcccggca gatcatcgag gaagggcata agctgggagc gatggaatcc
     1021 aagaatattg cagtggcgac actctctcac tatatcaata atgctgcatc tgctatctcc
     1081 ggacgtattc agatagtgga aatgctcttg aagaatgata tgatcgtcga taagcagggc
     1141 aagctgaccc cggcccttga ggttatcgat acctcactga agaaaatcct ggcggtgctg
     1201 gcagaactga agtcattgac ttctctcgac ggtgtaaagt tccacaatgg ctcgagcacc
     1261 atcaatatcg atgagaatat ctcacagcgc atcaagacga tgaaaaaagt catctgttaa
     1321 gaacccctga tttatcagat cctcccgtaa ttggtcaacc gttattataa gggctgctgg
     1381 gatacgatta tggcctttgc cgatatcctc gcagaagagg ggtacaatga atcccgctct
     1441 cgcttcttgc caaatttcct cgcaggtatt attatactat attaagattt acttgagaaa
     1501 ccagtatgga ggctaaatga agttcaattc acagattaca aaaatttcac aagtcagcgc
     1561 cgactgcctt gcagttcctg tctaccaatc cagatttgcc gataacccgc atctcaagct
     1621 ctttcccaaa gcgcttaaag atcatattca ggctctgttc aagggcgatg ttatcaccgg
     1681 cagggagggg gacgggtatc ttctcagggt cggaggaacg catggatttg aatatttata
     1741 tctagccggc atcggaaaaa aagaggatct ggagcatgat agcataagaa gattcggcgg
     1801 caatctggcc tccagcttaa agggcaagaa attcaaagag ttggccatct gtgttcccga
     1861 atcattccag ctggatgatg attgttcgct ggtctcccag atggctgtgg aggggctcct
     1921 gctcggctcc tatgaactgc ttaaatacaa gtccgagccg tcaaaaaaag cgatgcctgc
     1981 cagggtcagt ttcattctgc cggacagctg ggataagcgc agattcaatg ccggtttgaa
     2041 aatcggcgag ataatggcct ggggtcaaaa tctttcccgc gatctgatcg ggcatccctc
     2101 caattacctg acacccgtgg aattggcaaa taccgccaga tctatggcac gtacatatgg
     2161 ctttaagtgt gaggtactga ataaggcccg gatgaagaag ctgcgaatga attgcatcct
     2221 ggcggtcaat tccggatcca aagagccgcc acaattcatt attatgcact acaagggagt
     2281 gggctcgaaa aaggaccctg tctgcctagt cgggaagggg ataacatttg attccggcgg
     2341 gatctcgctg aagaaagcgc aggatatgga tcagatgaag ggcgatatgg gcggggccgc
     2401 ctgcgtgctc gcgtctgtag cggttgcagc ccgcttgaaa cttcccgtaa atctggtcgg
     2461 cctgataccc gccaccgaga atctcccctc cggttcggcc tacaagccgg gggatatcct
     2521 gacagcgatg aacggcaaga cgatcgaggt gctgaatacc gatgctgagg ggcggctggt
     2581 tctggccgat gcgctttcat attcgcagcg ccttaagccg gcggccatca tcgatgtggc
     2641 cacactgacc ggggcttcga tggtagctct gggatatgtc agctcggcca tgtttaccaa
     2701 tgatgacaaa ctgcgcaagc tgattatgtc tggctcacag aaatcgagcg agaagacctg
     2761 ggagatgccc ttgtggaagg agtatcagga gcagatcaaa tcctcgattg cggatatcaa
     2821 aaataccggc ggacgtccgg ctggttcatg caccgcggcg gcattcttaa ataattttac
     2881 cgccgattat ccctgggctc acctggatat tgccgcagtt gacgtcgttt tcgaaaaagg
     2941 tccctacaat cccaaaggtg cgagcggcta tggagtgagg ttgataagcg aattcctgcg
     3001 cgaatggaaa tagagactcg agaataaaaa aaaccgccca ctgccgggcg gtttttttat
     3061 gctcatatat tcaatccgct attttgtcca ggttggattg gagtcgttac cgggaccgga
     3121 aattcgtatc attcctgaac catccagatt aaccgtatat attgcaccat tggatgtgcc
     3181 gaaagcgatt ttattgccgt ccggcgacca cgaggccata ccggttatcc acagaccctc
     3241 ttccggcttg cagaccagtt tttctccgct caggtcggca ttggcggtat agactccttc
     3301 gctttgcagg aagagaatct tggttccatc aggcgaccag tccggagaac ttccgtcggg
     3361 attgtttgat gcggtctcga ccagaacgtc agttgcggga agttccccct caagcggaag
     3421 cacaaccaga cccagcttct tcattcccga ggactgcagt tctcccagaa caaaacagac
     3481 cttactgcca tccggcgaaa tggacggcat aagagggaat agctgttttt catcctgggg
     3541 catctccata tggctgaatg tctccgtctc gggatcataa aagccgatag aatattccgg
     3601 cacgcttgcg tccacaacat ttcccattct atcgtatgtg aagcagattg ttttgccatt
     3661 atcggaccag tcgggcgatt gtgacccgag agtattggtg aagcccatcc agaaattctt
     3721 cctttccgga aggctgtccg ggaaggcata aaaaagatca tataaataat gatatccgcc
     3781 gccgccttca ggaaatttaa tgtcaacttt gccctggcgg ctgaaggcca gccggccgtt
     3841 atcaggcgcc caggacatac ggcccttggc gttggaaatt cccaccacga aggccctctg
     3901 gtctgagccg tccggattca tgagccagac atcgccgtta cgtataaagg ctatctgttc
     3961 ccccatcgat tcggattctg aaccctgagc atgagaggat ccggctaaat aaatagaggt
     4021 cattaatgta aatacaaaaa acaaggttaa taaactgaat ctcttcattt gcagtccttt
     4081 cgtttcccgt ttatacactc attcagtctc cccgttgcgc attttttgga ttaaatccga
     4141 gcttttagat tcctgtcgat tcccagctaa ctcattgcag tataatcaaa aggcagccat
     4201 tatgactgcc tttttgttat cggaaatata atcaagaacc ttattcgctt ccatttccgc
     4261 tcatgctgta atccattgcg gccatctgcc tgtaaatgtt ccacctgcga tttgcatcct
     4321 gctgtgccag cttcatcagt tttgcagctc tctcgggatc ggagaccttc agtgttctcc
     4381 atctgttctc gcggtaagcg tactcctcgt atggaataga gggctctttg gaatccagtt
     4441 gcagcgggtt ttttccctgg tcgatcaggg ccggattgta tctgtaaaga atccagtgtc
     4501 ccgatttaac cgcattggtc atctcatcca gaccgttgga catattgaat ccgtgagcta
     4561 tacag
//
LOCUS       DSEB01000456            4982 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001331, whole genome shotgun sequence.
ACCESSION   DSEB01000456 DSEB01000000
VERSION     DSEB01000456.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001331"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(178..1695)
                     /locus_tag="ENO07_08095"
     CDS             complement(178..1695)
                     /locus_tag="ENO07_08095"
                     /inference="COORDINATES: protein motif:HMM:PF03264.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01966.1"
                     /translation="MFKKYISFIRGVSVNKLGLTGVVLTTSAFVTFIILELTRVLGFI
                     TNAYVGLVNYMLFPALFVIGLIMIPIAWRKRKRETGKSTEQLLQEQFEKRETEGSFLG
                     SRIFRTIAIFTLINILFLSVLSSRMLHFMDEPHFCGTACHTVMNPEWVTYQASPHARV
                     KCVECHVGEGVDALVNAKLNGLWQIISLTFNLYEQPIPTPVYQLRPARETCEKCHWPD
                     KFYGQRIKRIVDYEYDSLSTPRYTTLSLKIDVGEADVRRGIHWHIAEENEIRYASLED
                     EREEMIWVDVRQPDGSFKRYKNTELVSEHESEFEDFRILDCVDCHNRATHIYEQPEKA
                     LNERIRKGLIDRSVPFIKKAALGAILPIYNDTASAREQIANHVRGFYQREYPGIFRQK
                     FAEIDSTIAVLQSIYARNIHPYMNIRWNSYPGHIGHRGTGGCFRCHNPNMIDEEGESI
                     PDDCTMCHSIAAYGGNTPYQYLEPVDTSSQDYIIHQYLKNEFLESAAEAKNNQEQ"
     gene            complement(1705..3030)
                     /locus_tag="ENO07_08100"
     CDS             complement(1705..3030)
                     /locus_tag="ENO07_08100"
                     /inference="COORDINATES: protein motif:HMM:PF13447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01967.1"
                     /translation="MKKIFILSALLCLLVFGAGQAVDPEVAGKCMTCHKEKSPGLYKQ
                     WYTSAHGIHKVVCLDCHGAKQGEPDAYMHEGALIATLVTPKDCGKCHKKEMEEVDNSY
                     HATAGLILESNDAYLAHVAAGHPIAILGCESCHGAKMEIDKDEYNKLSKECWPNSGIG
                     RLNPDGSKGSCNACHTRHGFSKAQARQPEACSKCHLGPDHPQKEVYEESKHGNTYYTN
                     VDKMNLESDRWVVGEDYYVAPTCATCHMSATTNQPLTHDVGMRISWTLRPPISIKKDN
                     WQTKRKNMKDVCKSCHGSTFADGHYYQFDASVRLYNDKFVRPAEKILAMVHEKDLTEY
                     PANFANEIEWVYWELWHHEGRRARHGASMMGPDYTWWHGMYEVAKHFYFKMLPEARKV
                     EDAEINAYIDSLIANDPMHSWLTSETGELQERIRSGEMQKIYENYFQGE"
     gene            3312..4031
                     /locus_tag="ENO07_08105"
     CDS             3312..4031
                     /locus_tag="ENO07_08105"
                     /inference="COORDINATES: protein motif:HMM:PF13489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDS01968.1"
                     /translation="MDSLEDRLAQIDGRILDVAAGRGASLKYLVDKVGEFEQAVGVDF
                     SEQNLNQAKNQFDDNRIIFLKMRAEELDFDDNQFDLVAIVNSLHHLDNPQKSLEEMHR
                     VLKPGGLLLVSEMYNDNQSERQRSHVLLHHWWADVDRISGIPHYHTFPRQRIIDMIRN
                     LNLAEIQIMEYEEDKDLSGDKETMSFLIDATDNYLSRIMGKPEHRSLYQTGLELKERL
                     ATTGISWAKGIAIWGEKTLTR"
     gene            4138..>4982
                     /locus_tag="ENO07_08110"
     CDS             4138..>4982
                     /locus_tag="ENO07_08110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDS01969.1"
                     /translation="MRTNQNKICYSESNQDIFRIALLCSMGNYKGVIPMADIRKSLWL
                     LAFLITAGLFTPANAQQPTLVVTDQVVQMEFHDQITLVGRTQAWVESRLVSEVSGRVK
                     AIDAGEGIWIKRGTPLVTIDADQTRFILNAKQAETEQAKLEAELTKNDLKRMQDLYSK
                     DLISQSAYDSAATWASIKEQRYNQLESERKKLELDLKNSVIRAMYDGYTGRKLVDVGE
                     WVNPGMPVFEMVDLSRVKVTVDLPERHFGRVSTGSHATIVASGNGGEQLSGTVTGIAP
                     RASEET"
BASE COUNT         1312 a         1218 c         1174 g         1278 t
ORIGIN      
        1 gtttaatcga tgttagctaa tatagtccct gaaaatcaga ttccaaacgc aaaaaaagag
       61 acgctgttct gaacccaaag tttaacattc gccggcatgt tttagttcct ggcataaaat
      121 cgcgaataag cggctaaaaa tgaatgacga cgataactgc agcgcccctg ccgtctgcta
      181 ttgctcctgg ttattcttcg cctcggcggc ggattccagg aactcattct tcaaatattg
      241 atgtattata taatcctggc tggaggtatc caccggctcc agatactgat aaggagtgtt
      301 gccgccatag gcggcaattg aatgacacat cgtgcaatca tccggaatac tctcgccttc
      361 ctcatcgatc atatttggat tatgacagcg aaaacatccg ccggttcccc ggtgacctat
      421 atgaccggga taactgttcc atctgatatt catatatgga tgaatgttgc gtgcataaat
      481 gctctgcagt actgcgatag ttgaatctat ttccgcaaat ttctgcctga atattcccgg
      541 atattcacgt tgataaaatc cgcgcacatg attggcaatc tgctcccggg cggaagcggt
      601 gtcattatat atcggaagga ttgctcccag agccgctttt tttataaacg gcacggagcg
      661 gtcgatcaat cccttcctga ttctctcatt cagggctttt tcaggctgtt cgtaaatatg
      721 ggtggcacga ttgtgacagt cgacacaatc cagaatcctg aaatcttcga attcgctctc
      781 atgctcgctc accagttctg tattcttgta acgtttaaat gaaccgtccg gctgcctcac
      841 atctacccat atcatctcct cccgctcatc ctccagcgag gcataacgga tttcattctc
      901 ttcggcaata tgccagtgta tccccctgcg cacatccgcc tcgccaacgt caattttcag
      961 gctgagcgtg gtgtagcggg gtgtagacag gctatcatat tcgtaatcaa caattctttt
     1021 aatccgctgg ccgtagaatt tatccggcca gtgacatttt tcgcaggtct cacgtgccgg
     1081 acggagctga tataccgggg tcgggatagg ctgttcgtag agattgaatg tgagtgaaat
     1141 tatttgccag aggccgttga gtttggcgtt cacaagcgca tccactccct cgcctacatg
     1201 gcattccaca catttaaccc gcgcatgcgg cgaggcctgg taggtaaccc actcagggtt
     1261 catgacagta tggcaggcgg ttccgcagaa atgcggctcg tccataaaat gcagcatccg
     1321 gcttgagaga acgctcagga acaatatatt gattagggtg aaaatcgcga ttgtacgaaa
     1381 tatccgggaa cccaggaagc tgccttcggt ctcccgcttt tcaaactgct cctgcaaaag
     1441 ctgttcggtt gattttccgg tttctctttt ccttttcctc caggcgatcg gaatcattat
     1501 gagaccgatt acgaagagag cgggaaacag catgtaatta acaagaccca cataagcatt
     1561 ggtaatgaat cccaggaccc gcgtcagctc cagaattatg aatgttacaa aggccgaggt
     1621 ggtcaaaacc acgcccgtaa gcccgagctt gttgaccgaa acgccgcgga tgaaacttat
     1681 atactttttg aacaaactct gcccctactc tccctgaaaa taattctcgt agatcttctg
     1741 catctcccct gacctgatcc tctcctgcaa ctcgcccgtc tcagaagtga gccacgaatg
     1801 catgggatca tttgcgatca ggctgtctat gtaagcattg atctccgcgt cctcgacctt
     1861 tctggcctca ggaagcatct tgaaatagaa atgcttggcg acctcataca ttccatgcca
     1921 ccaggtataa tccggaccca tcatggaggc accatggcgc gcgcgtcgcc cctcgtgatg
     1981 ccagagttcc cagtaaaccc actcgatctc gttggcaaaa ttggcagggt attcggtcaa
     2041 atctttctca tgtaccatgg ccagtatctt ctctgcgggc ctgacaaact tatcattata
     2101 aagccggacg gacgcatcaa actgataata atgaccatct gcaaaggtcg agccatgaca
     2161 tgatttgcat acatccttca tatttttccg tttggtctgc cagttatcct tttttataga
     2221 aattggcgga cgaagggtcc agcttatgcg cataccgaca tcatgagtaa gcggctgatt
     2281 cgtggtagcg gacatatggc aggtggcgca tgtcggggct acatagtaat cctcccccac
     2341 cacccagcgg tccgactcca gattcatctt gtccacgtta gtataatatg tattgccgtg
     2401 tttggattct tcatagacct ctttctgggg atgatccggc ccgagatggc atttggagca
     2461 ggcctccggc tggcgggcct gggcctttga aaatccatgc cgcgtatggc aggcattaca
     2521 ggagcctttg gaaccgtcag gattgagcct gccgatcccg gagttgggcc agcattcttt
     2581 ggaaagctta ttgtattcat ccttgtcaat ttccatttta gcgccgtggc aactctcgca
     2641 gcccagaatg gcgattggat gtcccgcagc tacatgggca agataggcat cattcgattc
     2701 cagaatcagg cccgcggttg cgtgatagga attgtccacc tcctccattt ctttcttgtg
     2761 acacttcccg cagtcctttg gagtcaccag ggtggcgatc aaagcccctt cgtgcatgta
     2821 ggcatccggc tcaccctgtt tggcgccgtg acagtcgagg cagaccacct tgtgaatacc
     2881 atgcgccgat gtgtaccatt gcttataaag ccccggcgat ttttccttgt gacaggtcat
     2941 gcatttaccc gccacctccg ggtcaacagc ctgccctgca ccgaatacaa gcaggcaaag
     3001 caacgctgac agaataaata tctttttcat ggctttgctc ctccccaata tgctaaaata
     3061 acttttgcaa aatatttcga attatcctgc cctgtccctc gttatcaaag tacaggccat
     3121 caatattccc caatatagat tttttataaa attcggtcaa caatgaagat atttccttga
     3181 tctgaatcaa gaccttgtcc ggacgaagaa tttcggcctt acttttcccc tgaaatgcta
     3241 aatacacttg aatcatattt ctccagtcga cgtattcaaa taaattgaag caattttgat
     3301 ggagcttgca gatggactcg ttggaagaca gactggccca gattgacggc aggattctcg
     3361 atgtcgcggc gggcagggga gcatcactga aatacctggt agataaagtc ggagaatttg
     3421 aacaagctgt gggagttgat ttttccgaac aaaatctaaa tcaggcaaaa aatcagttcg
     3481 atgataatag aataatcttc ctgaaaatga gagcggaaga gcttgatttt gatgacaatc
     3541 aatttgacct ggttgcgatt gtgaattcac tgcatcacct cgataatccg caaaaaagcc
     3601 tggaagagat gcacagggtg ctcaaaccgg gagggctcct gcttgtgtcc gagatgtata
     3661 atgacaatca gagcgagcgg caaaggtcgc atgttctcct gcatcactgg tgggcggatg
     3721 ttgaccggat atccgggatt ccgcattatc atacatttcc gcgtcagcga attatcgata
     3781 tgataaggaa tttgaatctg gcagaaatac aaatcatgga gtatgaagag gacaaggacc
     3841 tttccgggga taaggaaacc atgtcattcc tgatcgatgc aaccgataat tatctttccc
     3901 gaattatggg taaacccgaa catcgcagtc tgtatcaaac cggccttgaa ctgaaagaac
     3961 gactggcaac gacaggaata tcctgggcaa aagggattgc aatctggggg gaaaaaaccc
     4021 taacccgata atcccgacca tgcgcctgtg gattaatccc atgaaactca acacattaca
     4081 agcacgatgc tgattatcat ccgattttga tattttacat tccaaaagtg atcttaaatg
     4141 agaactaatc aaaataaaat atgttatagt gaatcaaatc aagacatatt tcgtattgct
     4201 ttattgtgta gtatgggaaa ttataaagga gttattccga tggcagatat aaggaaatca
     4261 ctttggcttt tggcattttt gataaccgcg ggactgttca cccccgccaa tgcccagcaa
     4321 cctactctgg ttgtcaccga tcaggtggtg caaatggaat ttcatgatca gatcacgctt
     4381 gtgggacgca ctcaggcctg ggttgagagc cggttagtat cggaagtttc gggccgggtg
     4441 aaagctatcg atgccgggga gggaatctgg atcaaaagag ggacaccgct ggttacaatc
     4501 gatgccgatc aaacaagatt tatactcaat gccaaacagg ccgagaccga gcaggccaaa
     4561 cttgaagccg agctgacgaa gaatgatcta aaaaggatgc aggacctgta ctccaaggat
     4621 ttgatttcgc aatccgctta cgacagcgcg gcaacgtggg ccagtataaa agagcagcgt
     4681 tacaaccagc tcgagtcgga gcgaaagaaa ctcgaactgg atctcaagaa tagcgtcatc
     4741 agggccatgt atgatggtta cacgggccga aagctggtag atgttggaga gtgggtcaat
     4801 ccgggcatgc cggtatttga aatggtcgat ctctccagag tcaaggtcac ggttgacctc
     4861 cccgaaagac atttcggccg ggtatcaacc ggaagccatg caacgatagt agcctcaggc
     4921 aatggcggtg aacaactatc gggaacggtt acaggaattg ctccgcgggc ctcggaggaa
     4981 ac
//
LOCUS       DSEB01000457            4981 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001332, whole genome shotgun sequence.
ACCESSION   DSEB01000457 DSEB01000000
VERSION     DSEB01000457.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001332"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1304
                     /locus_tag="ENO07_08115"
     CDS             <1..1304
                     /locus_tag="ENO07_08115"
                     /inference="COORDINATES: protein motif:HMM:PF12698.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDS01970.1"
                     /translation="RMQGRRCPMHKLFAVIAREYKEIVKKKSFLVGTLLTPLFMIAII
                     FLPTLFIDRETKDPIEFTLVDLGSGLISEFKEAFGVTLPDGRPMYIVDYIRSSPEQID
                     ELKAELSLQVDEGTLNFYVIIPEDIIETGYAERYAKKRGSFADIKSIENTISGVVVRE
                     RLSQFKVPPEEIMAATRDINLEYKQVGPEGEERGGGNFLTQYLSGIAFVMILFTSIMG
                     YGQHLMRAVMEEKNTRIMEVLVSSLTPFQLMMGKILGLGAAGITQMALWAILGSSVFL
                     FAGSSDFVSGMLANAEALSINFFLSFIAFFILGYFLFATLFALLGSIVSSEKEVQQFV
                     APITMILVIPIILAMYIMQNPDAPWIVAVSYIPFLTPTMMILRASFTFVPPAEIIIGI
                     VVMIISILLLGAVTARIFRVGILMYGKRPTLPELVKWVKYK"
     gene            1409..1924
                     /locus_tag="ENO07_08120"
     CDS             1409..1924
                     /locus_tag="ENO07_08120"
                     /inference="COORDINATES: protein motif:HMM:PF08534.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDS01971.1"
                     /translation="MPRESGEAFMTMNTRIRNIDFELPSTDGHFQELSKRLVESPVLL
                     IFFKHDCPTCEFTLPYLERIYSRYGDSKAAFWAIAQDTESEAARFSEEHGISYEVLLD
                     RKPYEVSRKFDINIVPTVVLLDRDGSEISRFAGFQKAELEKLNYILAGLDNGTKPLFT
                     ASDDVPAVKPG"
     gene            2107..2985
                     /gene="pdxS"
                     /locus_tag="ENO07_08125"
     CDS             2107..2985
                     /gene="pdxS"
                     /locus_tag="ENO07_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HDS01972.1"
                     /translation="MKNSDNYKIKVGLAEMLKGGVIMDVTTPEQAKIAEDSGAVAVMA
                     LEKVPADIRKEGGVARMAAIEVIEQIKEVVSIPVMAKVRIGHFAEAQMLEAMEVDFID
                     ESEVLTPADDKYHIDKWDFKVPFVCGCRNLGEALRRISEGAAMIRTKGEAGSGNIVEA
                     VRHMRLVMGHIRKLQNMNPEELKSEAREMGVDVELVKKTAELGKLPVPNFAAGGIATP
                     ADASLMMQLGAESVFVGSGIFKSDDPAVRAKAIVQATTHFRDPKVLIEVSRHLGGAMK
                     GLDINKIPEDELLQIR"
     gene            3062..3667
                     /gene="pdxT"
                     /locus_tag="ENO07_08130"
     CDS             3062..3667
                     /gene="pdxT"
                     /locus_tag="ENO07_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HDS01973.1"
                     /translation="MVEKKIKLVRTDEKIYEVGILALQGDFELHTQALKRTGHESLLV
                     KTPDELKRVKRLIIPGGESTTVNKLVDIYNLRRPLIEFGRKNPVWGTCAGLIMLARDS
                     NDPRVQPFELIDIDAARNAYGRQVHSFSKKSKISLNGRSEEIELVFIRAPKITRVGDN
                     VQVLAELDGEIVMAREKNILVSAFHPELSESTLIHEYFLTM"
     gene            3844..>4981
                     /locus_tag="ENO07_08135"
     CDS             3844..>4981
                     /locus_tag="ENO07_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDS01974.1"
                     /translation="MADDRKIYFDHQATTPVDYRVLEAMIPYFSERFGNPSSHIHKEG
                     VRANQALDFARERVAELVNAKSEEIIFTSCATESNNLAVRGFLKANAKKGRHIIISEI
                     EHYSILNLAMKLKQEGYDVTTIKVDRRGLIDPQAVSGAIRDDTAMISINLANSEIGTI
                     QPIKEIAAIARERGIFMHTDGAIAVGNIPVDVESLGVDALTMSAHNYYGPKGVAALYV
                     RKGNAISPLFEGGFQEYGLRSGTENMPGIVGMGAASALALDEMPARIEHLTKLGKMLW
                     KGLEEEIEYINFTGHPEKRLPGHVSFWIKYIEGESLLLMLNIKGVMAASGSACSSNLR
                     GEDEEDLAASHVLTAIGVPVEFCSGSLTISMGKDNTEEEVLYLLD"
BASE COUNT         1281 a         1073 c         1395 g         1232 t
ORIGIN      
        1 gaaggatgca agggaggagg tgtccaatgc ataagctgtt tgcggtaata gcacgcgagt
       61 acaaggagat agtaaaaaag aagtcatttc tggtcggtac acttctgaca cccctgttca
      121 tgatcgctat tatatttctt ccgacgctgt ttatagatcg cgagaccaaa gacccgatag
      181 aatttacgct cgttgacctc ggatcgggct tgatcagtga attcaaggaa gcctttgggg
      241 ttacgcttcc cgacggccgt ccgatgtata tagtcgacta tatcagaagt tctccagagc
      301 aaattgatga gctgaaagcg gagttgagcc ttcaggtgga cgaggggacg ctcaattttt
      361 acgtgattat accggaagat atcattgaaa ccgggtatgc ggaacgctac gccaaaaagc
      421 ggggcagctt tgccgatata aagtcaatcg agaataccat cagcggggtg gtggtaagag
      481 aacgtttgtc gcagttcaaa gtgcccccgg aagaaattat ggctgctacc cgtgatatta
      541 acctggaata taaacaggtt ggtcccgagg gggaagagag gggcggtgga aatttcctca
      601 ctcagtatct ttccgggata gccttcgtga tgatattgtt cacatccata atgggttacg
      661 gacagcatct gatgcgcgcg gtgatggagg agaagaatac tcggattatg gaggtgctgg
      721 tatcgtcact gacgccgttt caactgatga tgggcaagat actggggctg ggcgcggcgg
      781 ggataaccca gatggccctg tgggccatcc tgggatcatc agtcttcctg tttgcgggca
      841 gttcggattt tgtgtcaggc atgctggcta atgccgaggc attgtccata aacttcttcc
      901 tgagctttat tgcttttttc attctcggtt atttcctttt tgctacgctt tttgcgcttc
      961 tgggatcgat cgtatcatca gaaaaggaag ttcagcagtt tgttgcgcca atcacgatga
     1021 tcctggttat tccgataatc ctggccatgt atatcatgca gaatcctgat gcgccctgga
     1081 ttgtggcggt atcttatatt ccgttcctga ccccgacaat gatgatattg cgcgcatcct
     1141 ttacgtttgt gccgccggct gagattataa tcggaattgt cgtaatgata atatcgatcc
     1201 tgcttctggg cgctgtaacc gcccgtatct tccgggtcgg aatcctgatg tatggcaagc
     1261 ggccgacact tccggaactt gtcaaatggg taaaatataa gtaaaattgc agcttaaatg
     1321 gtgccgatcg ttccggaagc gctgaaacaa tgggatggcc gctgctgttt aatatgaggg
     1381 gagatcagat tcgattgtca ggcgctggat gccgcgagaa tccggggagg catttatgac
     1441 tatgaatacc aggataagaa atatagattt tgaacttccc tcaaccgacg ggcattttca
     1501 ggaactgtcg aagcgtctgg tggagtcacc ggtactgttg atctttttca aacatgattg
     1561 cccgacatgc gaatttaccc tgccatattt ggagcgcatt tattcccggt atggtgacag
     1621 caaggcggct ttctgggcga ttgcacagga taccgaaagc gaagccgcac ggttttccga
     1681 agaacatggt atatcctatg aggttctttt ggacaggaaa ccctacgagg tgagcagaaa
     1741 gttcgatatt aatatcgtac ctacggtcgt tttgcttgat cgcgacggca gcgaaatatc
     1801 gaggtttgcc ggatttcaaa aggcggaact cgaaaagctc aattatattc tggccggcct
     1861 ggataacggc accaaaccgc ttttcaccgc atctgacgat gttcctgcgg taaaaccggg
     1921 ttgaggagcg aggcatactg cttagtttaa gcagcacttg ggattagcaa cacatttttt
     1981 gaagcgttaa tgcaaattgc agcgttaata atctggtata aaaattggag tcgggtattt
     2041 ccttttgaat aaaattttgt tatattttat gattggatgt tttttgatag aaacgaggta
     2101 aggcaaatga aaaactctga caactacaaa ataaaggtcg gtctggccga gatgctcaag
     2161 ggtggtgtga ttatggatgt tactactccc gagcaggcca agatcgccga ggattccggc
     2221 gcggttgctg tgatggctct ggaaaaagtc cctgcggata ttcgaaagga aggcggcgta
     2281 gcccgtatgg ccgcgatcga agttatagaa cagatcaagg aggtcgtatc gattccggtt
     2341 atggccaagg ttcgtatcgg gcattttgcg gaagcgcaga tgctggaggc gatggaagtc
     2401 gattttatcg atgagtcgga ggtgctcacc ccggccgacg ataaatatca tatcgataaa
     2461 tgggacttca aagtgccgtt tgtgtgcggc tgccgtaatc tgggcgaagc cctgcgcaga
     2521 atcagcgagg gagcagccat gatccgcacc aagggtgagg ccggttcggg caatatcgtc
     2581 gaagccgtgc gccacatgcg cctggtgatg ggacatattc gcaaactgca gaatatgaat
     2641 cctgaagagc tgaaaagcga ggcgcgcgag atgggtgtgg atgttgaact tgtgaagaag
     2701 actgccgagc tcggcaaact gccggtaccc aactttgcag cgggcggaat tgcaactccc
     2761 gccgatgcct ctttgatgat gcaacttggc gcggaatcgg tctttgtcgg atcagggata
     2821 tttaaatccg acgatcccgc tgtgcgggcc aaggctattg tgcaggcaac tactcatttc
     2881 agggatccga aggtgctgat tgaagtctcc cgtcatcttg gaggagcgat gaagggcctg
     2941 gatatcaata agatcccgga ggatgagctc ttgcagattc gctaaattgc gctggaaaac
     3001 gatgacgaga tattaacgcc ggattgagaa atgattcggc gttatttgat tgagaatgaa
     3061 aatggttgag aagaaaataa agctggtacg gaccgatgag aaaatttatg aggtcggaat
     3121 cctggctctc cagggcgatt ttgaacttca tactcaagcc ctgaaaagga cagggcatga
     3181 atccttgctg gtgaagaccc cggatgagct gaaacgggta aagaggctga ttatccccgg
     3241 aggtgaatcc accacggtca acaagctcgt cgatatctat aatctccgaa gaccattgat
     3301 tgaattcggc agaaaaaatc cggtttgggg gacctgcgcc ggccttatca tgctggccag
     3361 agattccaac gatcctcgag tgcagccatt tgaattgata gacatagatg ctgcccgcaa
     3421 tgcctacggg cggcaggtgc attccttctc aaaaaagagc aaaatcagcc tgaacgggag
     3481 atcggaagag atcgagctgg tgttcatccg ggctcccaaa attactcgtg tcggcgataa
     3541 tgtccaggtt ctggcagaat tagatggcga gatcgttatg gcaagagaga agaatattct
     3601 ggtctccgcc tttcacccgg agttgtcaga gagcacattg atacatgaat atttcttaac
     3661 gatgtaagct gcatttttag ttcggctgat gccgccaggc cttcaaggca gggattgagc
     3721 caaaagattc ccttacagga tcaatacttt gctgaatgct cgcaattggt agagcttgat
     3781 tttgcttgca gtttggatcg atttctgtta caatccaaga ggacaaaatg aatcgaggtt
     3841 taaatggctg atgacaggaa gatatacttc gaccaccagg caaccacccc ggtagattac
     3901 agagtgctgg aagcgatgat accatatttc agcgagcgct ttggaaatcc gtcatcacat
     3961 atacacaaag agggggtcag agcgaatcag gctctcgatt tcgcgcgcga gagggtagct
     4021 gagctggtca atgcgaagtc ggaggaaata atcttcacct cctgtgcgac cgaatcaaac
     4081 aatctggcgg tgcgggggtt tttgaaggcc aatgctaaga aaggccggca cataatcatc
     4141 tccgagatag agcattattc gatactgaac ctggccatga aacttaaaca ggaaggttac
     4201 gatgtcacga ccataaaggt cgaccggagg ggactgatcg acccgcaggc ggtttccgga
     4261 gcaatcagag atgacacggc gatgatctcg attaatcttg ccaattcgga gatcggcacc
     4321 atacagccga ttaaagagat agccgcaatt gcccgagagc gggggatttt catgcatacg
     4381 gacggtgcca tcgctgttgg aaacataccg gttgatgtgg aatccctggg cgttgatgca
     4441 ttaacgatgt cggcccataa ttactatgga cccaaagggg tggccgcgct ttatgtcaga
     4501 aagggaaatg ctatcagccc cctgtttgaa ggcgggtttc aggagtacgg tctgcgttcg
     4561 ggtaccgaaa atatgcccgg tattgtcggc atgggtgcgg catcggcact ggcgctggat
     4621 gaaatgccgg cgcgaatcga acatctgaca aagctgggga agatgctctg gaaggggttg
     4681 gaggaagaga tcgaatatat caactttacc ggtcaccctg aaaagcggct gcccggccat
     4741 gtctccttct ggataaaata tatcgaggga gaatcgcttt tgctcatgct gaatatcaag
     4801 ggtgttatgg ccgcatccgg ctctgcctgc tcctccaatt tgcggggcga ggatgaagag
     4861 gatctggcgg catcgcacgt tcttactgca atcggcgttc cggtggaatt ctgctcgggc
     4921 tcgcttacga tttccatggg caaagacaac actgaagagg aggtgctgta cctcctggat
     4981 a
//
LOCUS       DSEB01000458            4189 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001983, whole genome shotgun sequence.
ACCESSION   DSEB01000458 DSEB01000000
VERSION     DSEB01000458.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001983"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..819)
                     /locus_tag="ENO07_08140"
     CDS             complement(<1..819)
                     /locus_tag="ENO07_08140"
                     /inference="COORDINATES: protein motif:HMM:PF08309.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01975.1"
                     /translation="MLFPVNLSAQNFDDTDSILRLRSSSLWRGVNNLVVEDNLIYAVH
                     KSGFAVYDPQQGFLGVDTLSMVSLEHAYENSFRLFNEYLLYNRSGRVGFVFVKDPSRP
                     FVTAESDLQTSFLEADYRSLYLYLACGFEGVKVLYLGRRDNPVLETRLREPAHTVAVW
                     LHGEYLYAIDDYNGLFFYDISESPEEPRFIGKRLFVDQLKDMVTVGDIAYCANTDGGL
                     VLLDLSDPVDPQFIGRYETETVINTVEESGGYIFASDVYGNYEIYHPDSMEIVTY"
     gene            complement(892..>4189)
                     /locus_tag="ENO07_08145"
     CDS             complement(892..>4189)
                     /locus_tag="ENO07_08145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HDS01976.1"
                     /translation="QNTGQEYLGIERIPGLRNDTVAIRTGTPGADIHTVPARDKQGNQ
                     VKPLLVIIDTGLDTDHPDLADNLWTNPNELPDNGIDDDHNGFIDDIHGWDFSGDTIQF
                     FNLTGDNDVTDGMGHGTHVAGCAAAVSNNEIGVAGVASHTDLLGLKIFPNAFISISIR
                     AIMYAADMGADVINMSWGNYYQSRALREILEYAHSRNVLAVAAIGNFGDSAMTYPASY
                     PITMAVGATNSDDQVTYFSSYGSWMDMCAPGLDILSLRSDTLDMYAENGEPNVRIIDS
                     MYYLADGSSMAAPMVAGAAAEILSYSPGIVPDSLRKLLSMSADDYLDPYGRGDSLPGH
                     DVFSGWGRLNLSAALDLVEGRLAKIEFPASNRLIEGEAVLRGTAFSEFGEDFRLYAAP
                     ASDTLLQTLVSSGRADILHDELGRYGGWTEPGYYCFTLEVGANLYKRWVYYTAEPVIE
                     VRTPVNGDTVKGVLSFRGTVVAPGFEECRIYMSPAGDPSQREEIIFTSGYVADSLIGS
                     YSLSRTPEGEYVFDIVMQTADGEYSEDLSLFISNGFAQGFPTPGKGLLNYGPAIYDMD
                     GNGRLEAVVSSRTGIGAYNHLGGYVPGLWRTLGEENVEGPPAVFDINKDGYGEVAFVT
                     EGQLHLLRHDGYSMPGFPKDVPAERGQNGYPTVYFADMDSDGYEEIIYASMDGEIYAY
                     RHDGSSYFASLDGFFAETRGDMIEHIPVVFVEDFNLDGQKEMIVVMRNSISIFNTHNG
                     IEPDWLPGTEISDLTGITGACMADFDGDSLLELGIAGRETENEIIYVALMEVDGTFLH
                     PFPKYLERVNYLISYPAAADLDVDGKAEIIFTISPPIDVSEVWIVRSDGSTISSIGSG
                     EDNPFASFPGTMGPPAVADVDGDEFLDVIVRQGNFFPGRVNEAVYAFDQAGQILPGWP
                     VYTFADPNSVLYRLHMPSITNLGNDRDAIYADLLITADDSSIYAWELPVEYDGMEIAW
                     GHLLYDSRHSGILPPTFGKEPPAQSDSFSNPPQPAGFFLAQNYPNPFNAATRIQFRLM
                     RSSRVTLDIYNLLGQKIRTLADRNFGPGEHQIIWNGRDESGDEAASGVYFYRLQTNLG
                     TVSRKMIMLK"
BASE COUNT         1043 a         1184 c          979 g          983 t
ORIGIN      
        1 atatgttaca atttccatac tgtcgggatg atagatctcg taatttccat atacatcgga
       61 tgcaaaaata tagcctcccg attcctccac ggtattgatt accgtctctg tttcgtatct
      121 ccctataaat tgcggatcga caggatcact caaatccagg agaaccaggc cgccgtcagt
      181 attggcacaa taagcgatgt cgcccaccgt taccatatcc ttcaactggt ccacgaacag
      241 cctcttgcct atgaaccggg gctcttccgg actctccgat atatcgtaga aaaacaggcc
      301 attataatca tcaatagcat acaggtattc accgtgtaac caaactgcca cagtatgcgc
      361 cggttctctc agcctggtct ccagcaccgg attgtcgcgc cggcccagat ataaaacctt
      421 cacgccctca aatccgcagg ccaggtaaag atataatgat ctatagtcag cttcgaggaa
      481 ggaggtctgg aggtcacttt cggctgtaac gaacggcctt gacgggtcct tcacaaatac
      541 aaaaccgaca cggcccgagc gattatacag gagatactca ttgaacagcc tgaaactgtt
      601 ttcataggcg tgttcaaggg acaccatcga aagcgtatca acacccagaa agccctgctg
      661 cgggtcataa actgcgaagc cggatttatg caccgcgtag atcagattgt cttccacaac
      721 cagattgttc acaccgcgcc agagactgct gctgcgcaat ctcaggatgc tgtcggtatc
      781 atcaaaattc tgcgcgctca gattgaccgg aaataacatc aggagcagga gtagcgatga
      841 gcacaataaa aagcgagttt ttggtttcaa aataatcaat tttataattt cctatttcag
      901 cattatcatc ttgcgggata cggttccgag gtttgtctgg agcctgtaga aatagacgcc
      961 gctggccgct tcgtcgccgc tttcatcccg gccgttccag ataatctggt gttcccccgg
     1021 gccaaaattc ctgtcggcca gggtgcggat tttttgcccc agcagattat atatatccag
     1081 ggtcacccgg ctgctccgca tcaggcggaa ttgaatccgg gtagcggcat taaacgggtt
     1141 cgggtagttc tgggccagga aaaatccggc cggctgcggc ggatttgaaa aactgtccga
     1201 ttgcgctggc ggctccttgc cgaacgtggg cggaagaatt ccggaatgcc tgctgtcata
     1261 caggagatgt ccccaggcga tctccatacc atcatattca accggaagtt cccaggcata
     1321 gatcgaggaa tcatcggcgg tgattagaag atcggcgtaa atcgcgtccc ggtcattacc
     1381 gagattggtg attgagggca tatgcaggcg atagagcacg ctgttgggat cggcgaaagt
     1441 ataaaccggc catcccggca gaatctgccc ggcctggtcg aaagcataaa cagcttcatt
     1501 caccctcccg ggaaagaaat ttccctggcg cacaatcaca tccagaaatt catcgccatc
     1561 aacatcggct accgcaggcg gccccatagt tccgggaaag gaggcaaagg gattatcttc
     1621 tccgctcccg atactgctta tggtggatcc atccgaacgg acgatccaga cttcggaaac
     1681 atcgatcggc ggagatattg taaaaataat ttcagccttg ccatcaacat caagatcagc
     1741 ggcggcggga taactgatga ggtaatttac cctctccaaa tatttgggaa aaggatgcag
     1801 aaatgttcca tccacctcca tcaaggcaac atagatgatc tcattttccg tctcgcgccc
     1861 tgcaattccc agctccagca gggaatcgcc gtcaaaatcc gccatgcatg ctccggtaat
     1921 cccggtcaaa tccgaaatct ccgtccccgg cagccagtcc ggttcgatcc cgttatgcgt
     1981 gttgaagatc gaaattgaat tgcgcatgac cacgatcatc tccttctggc cgtccagatt
     2041 gaaatcctcc acaaagacga ccggtatatg ctctatcata tccccgcgtg tctctgcaaa
     2101 gaaaccgtcc aggctggcaa aatatgagct tccgtcatgc ctgtaggcat atatttcgcc
     2161 atccattgag gcgtagataa tttcctcata gccgtcactg tccatatctg caaagtaaac
     2221 ggtcggataa ccgttctggc ccctctcagc cggcacatcc ttgggaaacc cgggcatgct
     2281 gtagccgtca tgtctcaata aatgcagctg tccttcggtg acaaatgcca cctccccgta
     2341 gccgtcctta tttatatcaa aaaccgccgg agggccctcc acattttctt ctcctaacgt
     2401 gcgccatagt ccgggcacat atccgcccag atggttatag gccccgatcc cggttctgga
     2461 ggagaccacc gcctccagac ggccgttgcc gtccatgtcg tagatcgccg ggccataatt
     2521 caaaagccct ttgcctggag tgggaaatcc ctgggcaaaa ccattggaaa taaaaagact
     2581 taaatcttcg ctgtattcgc cgtcagcggt ctgcattacg atatcgaata catattctcc
     2641 ctcgggtgta cggcttaagg agtaactgcc gatcagcgaa tcggcaacat accccgaggt
     2701 aaatattatc tcctctctct gcgaggggtc gcccgcaggg ctcatataaa ttcgacattc
     2761 ctcaaatccc ggcgctacca ccgttcccct gaaagacagg acgcccttga cagtatcacc
     2821 attcacaggc gttctgactt caatcaccgg ttcggctgta taataaaccc agcgcttata
     2881 cagattggcg ccgacctcca gagtgaaaca gtaatacccc ggctccgtcc agccgccgta
     2941 tctccccaat tcatcatgaa gaatatcggc tcttccgctg gaaaccaggg tttggagaag
     3001 tgtatccgat gccggcgcgg cgtagagcct gaaatcctct ccgaattctg agaaagctgt
     3061 tccccttaga accgcttcac cctcgatcaa tctatttgac gccggaaatt ctattttggc
     3121 cagcctgccc tctaccagat caagagcggc cgaaaggttg agccttcccc atccactgaa
     3181 aacatcatga cccggaagac tgtctccccg accataggga tcgaggtaat catccgcaga
     3241 catactgaga agtttgcgaa ggctgtccgg gactatcccc ggagagtatg ataaaatctc
     3301 cgccgctgcc ccggccacca taggcgccgc catcgagctt ccatcggcca ggtaatacat
     3361 gctgtcgata attctcacat tcggctcccc gttttccgca tacatatcca gcgtatccga
     3421 gcgaagcgaa agaatatcca ggccgggcgc gcacatatcc atccaggaac catatgatga
     3481 aaaataggtc acctgatcat ccgaatttgt tgccccgaca gccatggtta tcggatagga
     3541 tgccggatat gtcatggcgg aatcgccgaa attgcctatg gccgcaaccg caagtacatt
     3601 tcgtgaatgg gcgtattcca gtatctcccg cagggccctg gattgataat aattccccca
     3661 gctcatatta atcacatccg cgcccatatc cgcagcatac ataatcgccc tgatgctgat
     3721 tgatataaag gcattgggaa atatcttcag gcccaaaaga tccgtatgcg aggctacacc
     3781 tgccacgccg atttcattat tgctcacagc cgcggcgcat ccggctacat gggtcccgtg
     3841 ccccataccg tcggtgacat cattatcgcc ggtcaggttg aaaaactgta ttgtatcgcc
     3901 ggaaaaatcc cagccatgta tgtcatcaat gaatccgtta tgatcgtcgt cgatgccgtt
     3961 atcgggcagc tcgttcggat ttgtccagag gttgtccgca aggtcgggat gatccgtatc
     4021 aagccccgtg tctattatga ccaaaagcgg cttgacctga ttgccctgtt tgtcgcgtgc
     4081 aggtactgta tggatatcgg ctccgggagt gccggtcctg atcgcgaccg tatcgttcct
     4141 caagccgggt atgcgctcaa ttcccaggta ttcctgaccc gtattttga
//
LOCUS       DSEB01000459            5132 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001232, whole genome shotgun sequence.
ACCESSION   DSEB01000459 DSEB01000000
VERSION     DSEB01000459.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5132)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5132)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5132
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001232"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..274)
                     /locus_tag="ENO07_08150"
     CDS             complement(<1..274)
                     /locus_tag="ENO07_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01977.1"
                     /translation="MRKAIYAALVIFLLSPAALYSQVNNPVIFSVEDTDSLLHAEFHF
                     SPLPRYDQICTLTVRLISRSEEVNAHAFKTPAIERKKPGSIRPEIFV"
     gene            complement(271..840)
                     /locus_tag="ENO07_08155"
     CDS             complement(271..840)
                     /locus_tag="ENO07_08155"
                     /inference="COORDINATES: protein motif:HMM:PF10263.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family peptidase"
                     /protein_id="HDS01978.1"
                     /translation="MKLSKKQQKALQATNVDMFFDLPVQHIETQQTVRLPDEKALSRI
                     YDTLNYRYFDGKLPKVKIEWSSRMRIAGKCYVEHRLIKLGRKYHEYFPDEVEDTLKHE
                     MIHILYPNHGKEFKREAERLGATRYAKEYPGGKSPHKYIYICPSCGQKYYRHRRMYNC
                     SCGICSIRGYDPRYKLKLYWSAKSRRRSR"
     gene            915..2180
                     /locus_tag="ENO07_08160"
     CDS             915..2180
                     /locus_tag="ENO07_08160"
                     /inference="COORDINATES: protein motif:HMM:PF05048.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01979.1"
                     /translation="MKSVYLIMFLCLPAVLFVSCAEKIEIAGSRTIIVDASSEVEEPD
                     GSEYAPYSSLEAAIAGADDGDEIYILPGQYEAEPQLFVEPLCGNCTEHRTEVRATSGF
                     HIKNKALTIIGKDADSVILITNAGYGILFDHSRGSSLTNVTITGGKRDPDGNATDAAV
                     VVRFSQVIVENCKIINNDHQLDTVVVGIGGVFGRESSQITIRGNLIENNGWDGIALYR
                     GASAFTSDNIINKGRGAGIGITWDSKAIVIRNDVSNYWKGIGTFGNSYAVVLNNVVHD
                     NLGWGVIATGFSMMEVRNNTIVQNGNCGFAAWSNEARGYLKNNIIAFNGWREEWVCPC
                     VGVWMNGKLANFPVSYNDVYDNAAGQYLGMPEWTGRYGNISVDPLFIDSLDFRLSPES
                     PLNAAGDPYYTNPDGSRSNIGAYGGQESK"
     gene            complement(2275..2712)
                     /locus_tag="ENO07_08165"
     CDS             complement(2275..2712)
                     /locus_tag="ENO07_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01980.1"
                     /translation="MFKHKLIIVVSAFLLAAGLSATGSAQVGPPAPDAIWADDVLYST
                     VGTPTSLPDHGPKDSLFVFQNLGGQVPVSESKPGDTDYNGGRWQVILLSFTPEGLGVH
                     DPDNDGESDLILTSWEQVKQHIDLGHLVRDGLGDSFVCPLKKQ"
     gene            2966..3874
                     /locus_tag="ENO07_08170"
     CDS             2966..3874
                     /locus_tag="ENO07_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDS01981.1"
                     /translation="MEVKGLRKNYNSLKAVDDVSFYIEKGEVFGLLGPNGAGKTTTVE
                     IMEGLRCATSGEVRIAGFDPRKNDFRFKQILGVQLQKSAMEERIKAREALQLYGSYYK
                     KSMPADELLELVGLSEKKNTYYQNLSGGQQQRLSLAIALVNDPEMVFLDEPTTGLDAQ
                     ARRSIWEIINKLRSEKKTILLTTHYIEEAEHLCDRVSIIDHGRIIAEGTPRELIDNSG
                     ISHHIEFKTENPVSDQTIKQLSEKHGNINLDGDIYSMKSDQAGKPLIHLIKLLEKEAN
                     ELIDLHLERPTLEDVFIKLTGRRIRD"
     gene            3871..4617
                     /locus_tag="ENO07_08175"
     CDS             3871..4617
                     /locus_tag="ENO07_08175"
                     /inference="COORDINATES: protein motif:HMM:PF01061.22"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDS01982.1"
                     /translation="MRQFFMFTWVATKILFRSREAVFWNFLFPVALFILYVTVFSGVY
                     PSEIPESRTVADNFSKILTITIMSGGLFSLAISVSVMKEKGILRRYKVTPVRPITIVS
                     GIVVRQYFFMLLVTAVLFLMAIIPYNADFGGGVLELFIIASLGILVFSSLGFCIAGIM
                     KTNQGTIGAANLLFMPMLFLSGAAIPVFLFPKWLKDIASVFPATHLLNILQRTLFEGK
                     ALAQDLKSLLILIGFGIVFLIVAAFVSRWN"
     gene            complement(4618..4940)
                     /locus_tag="ENO07_08180"
                     /pseudo
     CDS             complement(4618..4940)
                     /locus_tag="ENO07_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477707.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
BASE COUNT         1319 a         1206 c         1219 g         1388 t
ORIGIN      
        1 ctacaaaaat ctccggcctt atcgagccag gttttttcct ttcaattgcc ggggttttga
       61 aagcgtgcgc attgacttcc tcgcttcgag aaatcagtct gactgttagt gtgcaaatct
      121 ggtcgtaacg cggcagggga gaaaaatgaa attccgcatg cagcaggctg tcggtatcct
      181 cgacagagaa tatgacggga ttgttaacct gtgagtacaa agccgcggga gataaaagaa
      241 atattaccaa ggccgcataa attgcttttc tcatcttgac ctcctcctgc ttttggccga
      301 ccagtacagc ttcaatttgt atcggggatc gtaaccgcga atggagcata ttccgcagga
      361 gcaattgtac atgcgcctgt ggcgatagta tttttggccg cagctggggc aaatatagat
      421 atatttatgg gggcttttgc ctcccggata ctccttggcg tagcgtgttg cccccaggcg
      481 ttcagcctcg cgtttgaatt ctttgccatg attgggatag agaatatgta tcatttcatg
      541 tttgagagta tcctccacct catccggaaa atactcgtga tattttctgc ccagcttgat
      601 aagcctatgt tccacatagc attttcccgc gatccgcata cggctcgacc attcgatctt
      661 cactttcggg agtttgccgt cgaaatatcg atagtttaga gtatcatata tcctgctgag
      721 cgctttttcg tccggaagtc taactgtttg ttgggtttcg atgtgttgta ccggcagatc
      781 gaaaaacatg tcgacattag ttgcttgtag tgctttttgc tgttttttgc tcaatttcat
      841 gccctgaatt tattactatt ctatcttgca tacaagccga aattagctta gattcaaaaa
      901 atccgggagg gttcatgaaa tctgtctatc tgattatgtt tctgtgctta ccggcagttt
      961 tattcgtttc ctgcgcagaa aaaattgaaa ttgcaggcag ccgtactatc attgtggatg
     1021 cctcttctga agtggaagag cctgatggaa gtgaatatgc accttacagt tcgctggaag
     1081 ctgcgatcgc cggagccgat gacggcgacg aaatatatat tctgcccgga caatacgagg
     1141 ccgagccgca attattcgtt gaaccgctct gcggcaactg caccgaacat agaaccgagg
     1201 tcagggcgac ctccgggttt catatcaaaa acaaggctct gaccataatt ggaaaagatg
     1261 ccgacagcgt catcctgatt accaatgcgg gctacggaat cctgtttgac cacagccggg
     1321 gctcgagctt aacaaatgta acaatcaccg gcggaaagcg tgatcctgac ggaaatgcta
     1381 cagatgctgc ggttgttgtc aggttctctc aggttatcgt agaaaactgt aagattataa
     1441 acaacgatca tcagctcgat acggtcgtgg ttggaatcgg cggcgttttc ggccgggaaa
     1501 gttcccaaat cacgatcagg ggcaatttga tagagaataa cggatgggac ggtatcgctc
     1561 tttatagagg cgcaagcgca ttcacatcgg acaatatcat aaacaagggc agaggggcag
     1621 ggatcggtat tacctgggat tcaaaagcaa tcgtgatccg taatgatgta tccaattact
     1681 ggaaaggaat cggcacattc ggaaattcgt acgcagtggt attgaacaat gtggtccacg
     1741 acaatctcgg atggggtgta atcgccacag gtttttccat gatggaggtg cgtaacaata
     1801 cgatcgtgca aaatggcaac tgcgggtttg ccgcctggtc aaatgaggcg cgcggatatt
     1861 tgaagaacaa tattatcgcc ttcaacggct ggcgcgagga atgggtatgt ccctgtgtgg
     1921 gtgtctggat gaatggcaag ctggccaatt tcccggtatc atacaatgat gtttatgata
     1981 atgcggccgg gcagtacttg ggcatgcccg aatggactgg acgatatggc aatatttctg
     2041 tcgatccgct cttcatcgac tcgctggatt ttcgtctttc gcctgaatct cctctgaatg
     2101 ctgcagggga tccatattac accaatcctg acggcagtcg atctaacatt ggcgcttatg
     2161 gcggccagga atcaaaataa atctgacgat aatctggctt ttcaaaatga gaaaaaaggg
     2221 gagaccatta atctggtttc ccctttgtcg cttgaatcga atttgcgcct gatcctactg
     2281 ctttttcagc ggacagacaa agctgtcacc caaaccatcc ctgaccagat gacccaggtc
     2341 tatgtgctgt tttacctgtt cccagcttgt caatatgaga tcgctttccc cgtcattatc
     2401 gggatcatgg acaccaagac cctcgggggt gaagctgaga agaatcacct gccagcggcc
     2461 gccattataa tcagtatcac ccggcttgga ctctgataca ggcacctggc cgcccagatt
     2521 ctggaagaca aagaggctgt ccttggggcc atgatcgggc aggctcgtcg gcgtgcccac
     2581 cgtcgaataa agaacatcat ctgcccagat cgcatccggg gccggtggcc ccacctgcgc
     2641 tgaaccggtc gcgcttagcc ccgctgccag caaaaatgct gaaaccacga tgataagctt
     2701 gtgcttaaac atacatcctc ctttttaaga atgtggtttt ttgataagta ttctgactgc
     2761 aaccgtcatg ctgcatgcaa actaaaacgt accgctataa acaggacaac aattgtagtg
     2821 tattatatgt tccgcagcga ttatattgct gccggagaaa ttatgaatcg agtcgattct
     2881 aaacttgatt gtttcagttt tgtttgtatt atattagcat gtattggctt agatagaaaa
     2941 tgaggtcgtc ttgcagcaga aattcgtgga agtaaaaggg ctcaggaaaa actataattc
     3001 tcttaaggct gtcgatgatg tctcctttta tatagaaaaa ggtgaggtat ttgggcttct
     3061 tgggcctaat ggagcaggca agaccaccac tgttgagata atggaagggc tgcggtgtgc
     3121 caccagcggt gaggtcagga tcgccggatt cgacccgcga aaaaacgatt tcagattcaa
     3181 gcaaatcctt ggtgttcagc ttcaaaagtc agccatggag gagagaataa aagctcgcga
     3241 agcattgcag ctctatggat catattataa aaagtctatg ccagctgacg aacttctgga
     3301 attagtcggc ctctcagaaa agaagaacac ttattatcag aacctgtccg ggggtcagca
     3361 gcaaaggttg tctcttgcca tagcgcttgt caatgatccc gagatggtgt tcctcgacga
     3421 gcctactacc gggcttgatg cccaggcccg gcggagtatc tgggagatca tcaataagct
     3481 ccgcagcgaa aagaaaacca ttcttcttac aacacattac atcgaggaag ctgaacatct
     3541 ctgcgaccgt gtttcgatca tcgaccacgg caggatcatc gccgagggta cgccacggga
     3601 actgatcgac aactcaggta tctcacatca catagaattc aaaactgaaa acccggtaag
     3661 tgaccagaca attaagcagc tttctgaaaa acatggaaat attaatctcg atggggatat
     3721 ctacagtatg aaatccgatc aagccggaaa gccattaata catcttatta agctgcttga
     3781 aaaagaagcc aatgagctga tcgaccttca tctggaacgt cccacactcg aggatgtgtt
     3841 catcaagctg accggaagga gaatcagaga ttgagacagt ttttcatgtt tacatgggtg
     3901 gccaccaaga ttctctttcg gtcacgcgag gcggtcttct ggaatttcct ttttcctgtg
     3961 gcgcttttta tcctgtatgt caccgttttc tccggagtct atccaagtga aattcctgaa
     4021 tcccgaactg tggccgataa cttctctaaa atcctgacaa ttacgatcat gtcaggcggg
     4081 ctgttttcgc ttgcaatctc ggtttctgtc atgaaagaaa agggcattct caggagatac
     4141 aaagtaactc ccgtgaggcc gattacgatt gtctccggca tcgttgtgcg ccagtacttc
     4201 tttatgctcc tagtaaccgc ggtcctgttt ttgatggcca ttattccgta taatgccgat
     4261 ttcggcggcg gcgttctgga gttattcata atcgccagcc tgggcattct tgtcttcagc
     4321 agcctgggct tttgtattgc cggtataatg aagacaaatc agggaactat cggtgccgcc
     4381 aatctgcttt tcatgcccat gctgttcctg agcggggcag caattccggt attcttattc
     4441 ccgaaatggc tgaaagatat tgccagtgtc tttccggcca cacatctatt gaatatcctg
     4501 cagcgtacac ttttcgaggg caaggcgctt gcacaggatt tgaagagcct gctgatcctc
     4561 attggattcg gaatagtgtt tctgatcgta gccgcctttg tctcccgctg gaattaatta
     4621 atccctcagc gcatcaattt tggcagccat gatgaaatca ttctcagaaa ggccatttat
     4681 cgcatgggtc cagatctcaa attcggtctt gttgagtgca cacagaaatc cggatgatgc
     4741 ccctcgttct cggctaagtc cgcaacccta ttcagaaaat ccatattccg gacaaaatcc
     4801 ctgaatttat attctttata gagatgccca ttcttaactc tccagccctc cacctgcttg
     4861 agcatctgcg tgatcttctc ttcatcaaac ggttccacac cgccctcgca gggcacacac
     4921 tttttatctg tcaaagccat tttattcctc ctttttagct tcacaatatt gaagaacatt
     4981 tctgagaatg caatgtttct aaacagccca ataaaaaaac ccggcaggag ccgggcctgt
     5041 cagggatatt catatcatca tggtatcaca atcgtgtcct cgcgtcccgc accggtggag
     5101 ataatagtcg ctttgcaggc aacataatcc tc
//
LOCUS       DSEB01000460            2191 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1008283, whole genome shotgun sequence.
ACCESSION   DSEB01000460 DSEB01000000
VERSION     DSEB01000460.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1008283"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            545..1555
                     /gene="tsaD"
                     /locus_tag="ENO07_08185"
     CDS             545..1555
                     /gene="tsaD"
                     /locus_tag="ENO07_08185"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013379346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDS01983.1"
                     /translation="MRILGIETSCDETSVAVVEGMKKTRVNLVYSQVDHARFGGIVPE
                     IASRQQLKKLPLLYIQALNESGLELKDMDGIAVTMGPGLVGSLLVGVNFAKALSYSCG
                     LPLLGINHLEGHVFSNYLASPQLEPPFVALIVSGGHTILVHVCDYLDYQILGQTRDDA
                     AGEAYDKVSKLLGLGYPGGKIIDDLAGKGNPNFHKFPRAFLKDISYEFSFSGLKTAVV
                     LYVKEKGPEFVKNNLFDICASFQEAVVDVLVSKAMLAARSLEINKIVLAGGVAANSRL
                     REKLIFKAEKKKIEVFYPPPILCTDNAAMIASAGHKRFEKGQRSDFNLNAYPYLPLSG
                     SN"
     gene            1604..2101
                     /locus_tag="ENO07_08190"
     CDS             1604..2101
                     /locus_tag="ENO07_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HDS01984.1"
                     /translation="MNIEIIQGDITEQATDAIVNAANNQLWMGSGVAGAIKKQGGEEI
                     EKEAVQKGPIGIGQAVATTAGKLKAKYVIHAAGMGQDLVTSAEKVKQSTFSSLKVADS
                     LELKSISFPAIGTGVGGLGLDQCAESMLKAVFQFQPKHLQDIRFVLFDPTARQVFESV
                     YKKMK"
BASE COUNT          553 a          535 c          574 g          529 t
ORIGIN      
        1 acaccggacg ggtgccggtg gagccgctaa tctctgtcga acctgccctg ggatacacaa
       61 aatcagggga gcttcgggtc actcccgaca gcgttgaggt cacagggccg gcaaaattca
      121 taagggcgct gaaatctatt cctactgaag agataaaatt tcgggatgtc aaaaaagata
      181 tcgatgaaaa tgtgaaattg ataactcagg atacgatgtt cctgaacctg gagacccggg
      241 aggtcggtgt ccatctggat atagtgcctc ttgtagaaaa gagattcgga ccgattgcaa
      301 tagaaactta taattccgac ctgtttgact caaccgcctt tgaacccgat tcactctatc
      361 ttataatcga ggggcccgga gggcaattgg acacgctcga tgagacggct ttctccgcct
      421 ctataaactt ccgttcaatt gaagccggca gcaccacggt ccttcccaaa gttgtggttc
      481 cgcccggcta tcagctggtg cggaccgagc cgcattcaat ctcagtaact gccacttcta
      541 agccgtgaga atacttggaa tagaaacttc ctgcgatgaa acatcagtcg cagttgttga
      601 gggcatgaag aagactcggg tgaacctggt ttactcacag gtcgatcatg ccagattcgg
      661 cggcattgtt cccgagatag cttcccgcca gcagttgaag aaactgcccc ttttatatat
      721 acaggccctg aacgaatcag gcctcgagct caaagacatg gacggcattg cggttacaat
      781 gggcccggga ttggtgggat cacttttggt gggtgtaaac tttgccaagg cgctgtcata
      841 ttcctgcggc ctgccgcttc tcggaataaa tcacctggag ggacatgttt tctccaatta
      901 tctagcctcg ccccagttgg aaccgccctt tgtcgccctg atcgtctccg ggggtcatac
      961 aattcttgtg catgtttgcg attatcttga ttatcagatt ctgggacaga cccgggatga
     1021 tgccgcgggc gaggcctacg ataaggtctc gaaactcctg ggactcggct atcctggcgg
     1081 gaagatcatc gatgacctgg ctggaaaagg taatccgaat tttcacaaat ttccgcgcgc
     1141 gttcctcaag gatatatcat acgagttctc attctccggc cttaagacgg cagtagtgct
     1201 gtatgtgaag gagaagggac cggaatttgt gaagaataat ctgttcgata tctgcgcctc
     1261 gttccaggag gcggtcgtgg atgttcttgt ttccaaggcg atgctggccg ccaggagcct
     1321 tgaaatcaat aaaatcgtcc tggccggtgg cgtggcagcc aattccagac ttagagaaaa
     1381 attgattttc aaggctgaaa agaaaaaaat agaggtcttc tatccgcctc ccatattgtg
     1441 caccgacaac gccgccatga tagcctctgc cgggcacaaa cgctttgaaa aaggacagag
     1501 atcggatttc aatctcaatg cttatccata tcttcccctg tcgggatcaa attgatccac
     1561 tcgttcgttt gttttatatt gatagtaagc aaaaggagga gagatgaata tagaaatcat
     1621 tcagggagat atcacagaac aggcgaccga cgctattgta aacgccgcca ataatcagct
     1681 ctggatgggt tccggcgtgg ccggcgccat caagaaacag ggcggcgagg agattgaaaa
     1741 ggaagcggtt cagaaagggc cgatcggaat cggtcaggcc gttgccacaa ctgcgggaaa
     1801 actgaaggcc aaatacgtga tccatgccgc cggtatggga caggatctgg taacctccgc
     1861 cgagaaagtg aagcaatcaa ccttctcctc actcaaggtc gctgatagcc tggaactcaa
     1921 gtcgatttcc tttccggcca tcggcacagg ggttggcggg ctggggctgg atcaatgtgc
     1981 tgaatcgatg ctgaaggcgg tcttccaatt tcagccgaag catctgcaag atatccgctt
     2041 tgttctattt gatccgaccg ccagacaggt cttcgagtcg gtttataaga aaatgaaata
     2101 agaactgttg ttgggttcga agcggaaggt gatgctactt cttccggcct gattcctcag
     2161 cttccatctc ctgctccagc tcatcttcag g
//
LOCUS       DSEB01000461            2227 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1007978, whole genome shotgun sequence.
ACCESSION   DSEB01000461 DSEB01000000
VERSION     DSEB01000461.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1007978"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..764)
                     /locus_tag="ENO07_08195"
     CDS             complement(<1..764)
                     /locus_tag="ENO07_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01985.1"
                     /translation="MDLSRLDRRIIFLIIAVVVIVPMLFDITPEVQITKDTQQIFDAI
                     DSLNAGDYIMVSFDFEAASFPEMRPLASVILNHAFEKDLKIVGLALFSEGTAIGYTLL
                     EQIARNYNKTYGLDYVYLGFRPQYVSAILGMGESVIKVFPEDYLKQPLEIYTDFLGLD
                     NYEDLPLLVSIADGSLPIHWADYANARYGQEVAVACAAVMVTAFKPYFESGQLIGPAA
                     GVKGAAEYEKLLGYEGAAHRRLFTQSVVHLAIILLV"
     gene            complement(764..1387)
                     /locus_tag="ENO07_08200"
     CDS             complement(764..1387)
                     /locus_tag="ENO07_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01986.1"
                     /translation="MKTRLPIAICFIFGIFTLLQYFSKHPTLTAMYAEILDWMQIIFV
                     FTLLVGVITIFKIHSGKISRKESNWPYSILTVLGIVVMAGLGFGYGTDYGSPFLWIFQ
                     NIQVPMQSTMFALLAFFVASAAYRGFRFRNKEAAVLLITAIIVMLGRVPLGHQISDLI
                     PQTAEWIIDNPSTAAKRGILIGIGLGSITTALRVILGIERSYLGGRS"
     gene            complement(1389..2171)
                     /locus_tag="ENO07_08205"
     CDS             complement(1389..2171)
                     /locus_tag="ENO07_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01987.1"
                     /translation="MSDTPLFDTSRLPVFVASLLAIVIFFIATYLVKARAETKIRKVA
                     GLNAVDEAIGRATEMGRPVLFTPGWGGDIQRPTTIAALNILNLVAEKTAAYNCDLIFP
                     THDPVIMSAAEETIQNAYINAGHPDLFKPDNIYYTTSSQFGYAAAVDGAITRIKPATV
                     MLLGTFEGEALILAETANSIGAMQIAGTDSTIQLAFFLVACDYTLIGEELFAASGYLS
                     GDEKIVASIKAQDYMKIIIIAILLAGGILATFNLDFLSEWIL"
BASE COUNT          603 a          591 c          544 g          489 t
ORIGIN      
        1 attacaagca gaattatggc caaatgaacc accgactggg taaacagccg cctgtgggcg
       61 gcgccctcat atcccaaaag cttttcgtat tctgccgcac ccttgactcc agcagccggt
      121 ccaattagct gaccactctc gaagtaaggc ttgaaggctg tcaccatgac cgcggcgcag
      181 gcaactgcaa cctcctggcc gtaacgggca ttggcgtaat cggcccagtg aatcggaagc
      241 gaaccgtcgg caatcgatac caggagcgga agatcctcat agttatccag cccgaggaag
      301 tcggtataaa tttccagcgg ctgtttcagg taatcttccg gaaatacctt tataaccgat
      361 tcacccattc ccagaatcgc cgaaacatac tggggacgaa atccgagata gacataatcc
      421 aggccataag ttttgttata attccgggca atctgctcca gcagagtata gccgatcgct
      481 gtaccctcgg aaaaaagggc caatcctaca atcttcaggt ctttctcgaa agcatgattc
      541 aaaattaccg atgccagcgg acgcatctcc ggaaatgagg ccgcctcgaa atcgaacgag
      601 accatgatat agtcgccggc attaagggag tcgatcgcat caaaaatctg ctgtgtgtcc
      661 tttgttatct gcacctcagg cgtgatgtca aaaagcattg gcacgataac taccactgcg
      721 ataatcagaa atattatccg cctgtccagc ctgctcaaat ccatcaggac cttcctccaa
      781 gatatgaacg ctcgatcccg aggatcacgc gcagcgcagt ggtgatcgag cccaggccga
      841 ttccgatcag gattccccgt ttggccgcag tggaaggatt gtcgattatc cattccgctg
      901 tctgcggaat caggtcggat atctgatgcc ccagcggcac ccgtcccagc atgacgataa
      961 tggccgtgat caaaagcacc gccgcctcct tgttgcgaaa gcggaatccg cgataagcgg
     1021 cagaggccac aaaaaatgcc agaagcgcaa acatagtgga ttgcatcggc acctgtatat
     1081 tctggaatat ccacaaaaac ggcgatccgt agtcggtgcc gtatccaaag cccagcccgg
     1141 ccattaccac aatgccaagg acggtcagaa tactgtaggg ccagttggat tcttttctcg
     1201 atattttgcc ggaatggatt ttaaatatgg ttatgacgcc cacaagcaat gtgaacacaa
     1261 aaatgatctg catccagtcg agtatctcag catacattgc ggtaagcgtg ggatgtttcg
     1321 aaaaatattg caacagggta aatatcccga atatgaagca gatcgctatg ggcagacgtg
     1381 ttttcatgct ataaaatcca ctcgctcaaa aaatccagat taaatgttgc cagaatccct
     1441 ccggcaagca gtattgcaat tattataatt ttcatataat cctgcgcctt tattgacgcg
     1501 acaatcttct catccccgga aagatatccc gaggccgcaa agagctcctc ccctatcaat
     1561 gtgtagtcgc aggccaccag gaagaatgcc agctgaatgg tcgaatcggt gcctgcaatc
     1621 tgcatggccc cgatagaatt ggcggtctca gccagaatca gggcctcccc ctcgaatgtt
     1681 cccagaagca tgacggtggc cggtttgata cgcgtaattg cgccgtcaac cgcagcggca
     1741 tagccaaact gggatgatgt cgtatagtag atattgtccg gtttaaatag atcgggatgc
     1801 ccggcattga tataagcgtt ttgaatcgtc tcctcggcgg ccgacattat aaccgggtca
     1861 tgcgtcggga agatcaggtc gcagttatag gcggcggttt tctcggccac gagattcaat
     1921 atgttcaaag cggctattgt cgtcggacgc tgaatatccc cgccccatcc gggggtaaac
     1981 aacaccggac ggcccatctc ggtcgcccgt ccaatcgcct cgtcgacagc attcagcccc
     2041 gccaccttgc gtattttggt ctcggcccgc gcctttacga gatatgtggc tataaagaaa
     2101 atgacgatgg caagcaaaga cgccacgaag accgggagac gggaagtatc aaagaggggt
     2161 gtatcagaca aaacggcatc ttcaatttga gaatatgcca acagcggagc aaaaaggaca
     2221 ataaggc
//
LOCUS       DSEB01000462            2591 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1005705, whole genome shotgun sequence.
ACCESSION   DSEB01000462 DSEB01000000
VERSION     DSEB01000462.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1005705"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(87..1523)
                     /locus_tag="ENO07_08210"
     CDS             complement(87..1523)
                     /locus_tag="ENO07_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010133504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01988.1"
                     /translation="MSDILIIVPGSVLLSILIWFIILSFVLWAARTHAHRAIRSFGQI
                     LHSSMRLTAAAIMRAEKRLLERNREVLLSQGREAAERIIEREFDRIDATVRKDLAEYP
                     SLHRQLSEEITKIDEDYKESTEVPPEPPGWVKAVEAVAKIPATKGDPMVGNVLEDIHQ
                     SLVKANTRALEEYRKSSHKRHQLLNNMMPHWRNVKQILGKVDKNVNSLLQRSVTIDRH
                     MDEYENIQKGSNRALEMLSSSAITQFFISAFVLIIAIGGAMINFNLIALPMSEMVGAK
                     SEILGMPTSDVAALVIILVEIAMGLFLMESLRITRLFPIIGALNDKMRIRMVWITFTI
                     LLALACVEAGLAYMREWILEMKQASMITSGDDVVQNTSLWITTAAQMGMGFVLPFALV
                     FVAIPLESFVHSLRTVLGIIGVAFLRSLAWLLRFLGNISRYAARVVENIYDLVIFAPL
                     LIERLTSGSREAKKSGNKDKKIDTASAY"
     gene            1956..2032
                     /locus_tag="ENO07_08215"
     tRNA            1956..2032
                     /locus_tag="ENO07_08215"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:1990..1992,aa:Arg,seq:cct)
     gene            complement(2141..2473)
                     /gene="tusE"
                     /locus_tag="ENO07_08220"
     CDS             complement(2141..2473)
                     /gene="tusE"
                     /locus_tag="ENO07_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HDS01989.1"
                     /translation="MTTMTQLERDNEGYLIDPEQWNEEVARELAEEEGIELDETYLAV
                     LKFMRRYWAEHQVAPDVRHVVDYLVKEEGFDKKRAKAHLFELFPYGYVKQACKVAGMI
                     KPRAWSTG"
BASE COUNT          656 a          705 c          602 g          628 t
ORIGIN      
        1 gtattatgcc tcgaacaacc actaacaacg gccagcgcga ccactgtcat cagaataaac
       61 aaaccattct ttttcatttc tcccccttaa tacgcagacg cggtatcgat ttttttatct
      121 ttattaccgc ttttttttgc ttcacggcta cccgaggtaa gacgctcaat cagcaaaggt
      181 gcaaaaatca ccagatcgta aatattttcc actactctgg cggcgtaacg cgagatattg
      241 cccagaaaac ggagtaacca cgccaggctg cgcaggaaag cgacaccgat aatccccagc
      301 acggtacgca gcgaatgaac aaaagactcc agcggaatcg ccacgaatac cagagcaaac
      361 ggcagaacaa atcccatgcc catttgcgcg gcagtcgtga tccagagact ggtattttgc
      421 accacgtcat caccactggt aatcatcgag gcctgtttca tctccagtat ccattcacgc
      481 atataggcca gacctgcttc aacgcaagcc aaggccaaca gaatcgtaaa ggttatccag
      541 accattctga tccgcatctt gtcattcagc gcgccaataa ttggaaatag tcgggtaatg
      601 cgcaaagact ccatcaagaa cagacccatg gcgatttcca ccaggataat caccagggca
      661 gcgacatccg aggttggcat gccgagaatt tcactcttgg cgcccaccat ttccgacatt
      721 ggcagtgcaa taagattaaa gttaatcatt gcgccgccta tcgcgatgat cagcacaaag
      781 gccgagatga agaactgggt aatggcggat gatgacaaca tctccagcgc gcgattactg
      841 cctttctgga tattttcata ttcatccatg tgtcgatcga tagttaccga acgctgcaac
      901 aggctgttga cgttcttatc gaccttgccc agtatctgtt tcacatttcg ccaatgcggc
      961 atcatattgt taagtaattg atgacgcttg tgactggact tgcgatattc ttccagtgct
     1021 cgtgtattgg ccttgaccag cgactgatgg atatcctcga ggacattgcc caccatcgga
     1081 tcccctttgg tcgcagggat cttggctact gcttcgactg ccttgaccca gcccggcggt
     1141 tccggcggaa cttccgtgct ttccttgtaa tcttcgtcaa ttttggtgat ctcctcgctc
     1201 aattgacgat gcagtgatgg atactcggcc agatccttgc gcacggtagc atcaatacga
     1261 tcgaactccc tctcgatgat gcgctcggcg gcctccctgc cctgactcaa cagcacctcg
     1321 cgattgcgtt cgagcagccg tttctccgca cgcatgattg ccgcggcagt cagccgcatg
     1381 gaactgtgca atatctggcc gaatgaccga atggcgcgat gcgcgtgagt acgagctgcc
     1441 cacaacacaa aactcagaat aatgaaccag atcaatatgc tcagcaaaac gctacccggc
     1501 acaattatta gtatgtcact catttttatt tatctcctca catcctgtca gaacgcagcc
     1561 gctccaaccc ggggcatggt aatggtatcc agcaggacaa aactgataaa cggatcacag
     1621 aacatctgct gttgttttaa ttatcagtaa cttaacaaac atagtataga tgagccatca
     1681 gatgttgcta gcctcagatt aacagagtgt gacagtgaca gagtgatttg tgagacaggc
     1741 ccgcaaaatt ttcgctgctg gcgaagttca gtgaccatgg aagcgccagg ggaaggaaaa
     1801 tctgaatgcc gatcagcctg tctcgtcggc cggttgcagc ggaacccggt tccctggagg
     1861 actgtatccc gctgatatac agcgcagcat cacaaaagcc ggttttccgt gcgttgggtt
     1921 cgcccgatag cgatgctaca attaccggct atcgtgcccc gatagctcag ctggatagag
     1981 catccccctc ctaaggggaa ggtcagaggt tcgaatcctc ttcggggcgc caggcattat
     2041 taaaagggac aagagagccg gcgattttac agggggacga gacaaagctc accggctccc
     2101 ttgtgtactc tgttggaaat tgcggcaacg ccggttcgat tcagccggta ctccaggcgc
     2161 gcggcttgat catgccggcg accttgcagg cctgtttgac ataaccgtag gggaacaatt
     2221 cgaacaggtg agccttggcc cgttttttgt cgaagccttc ctccttgacc agatagtcca
     2281 ccacatggcg cacatccggg gcgacctgat gttcggccca gtaacggcgc ataaacttca
     2341 gcaccgccag ataggtctca tccagctcaa tcccttcctc ttcagccagt tcgcgggcga
     2401 cctcttcgtt ccactgctcg gggtcaatca aatagccttc attatcccgt tccagttgcg
     2461 tcatggtcgt catggaggac tcctcttgcc ttctctgtta atgtggtaaa aacgatataa
     2521 gtatcacata atacagtcaa acaatattat ttggactata atatcgatat attcgatatt
     2581 tgatatagtg a
//
LOCUS       DSEB01000463            2053 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1009512, whole genome shotgun sequence.
ACCESSION   DSEB01000463 DSEB01000000
VERSION     DSEB01000463.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1009512"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..430)
                     /locus_tag="ENO07_08225"
     CDS             complement(<1..430)
                     /locus_tag="ENO07_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01990.1"
                     /translation="MRRKILIMSIALICLTVSVLSAQKNNYRKADHALRAVYEEITGV
                     SLEEAIDQLIAEKREGRMADGNVIVRFNGGGTRNKLYIGRENTLEFLVENDYNIQGAS
                     LGFEFSCTGGPGSFSWVHGYGTHPASDPVLQTHFEPFVFYP"
     gene            829..1233
                     /locus_tag="ENO07_08230"
     CDS             829..1233
                     /locus_tag="ENO07_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017742794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDS01991.1"
                     /translation="MAEPDYGAKQSASGVFQTEFEFTLPKGYVDKDGNVHKKGIMRLA
                     NAKDEIAPLSDYRVQQNRAYLVIIILSRVITKLGNLSDINPGVIENLFSADLSYLQDF
                     YRKINEEGTATIATVCPACGHQYEVDMGSLGK"
     gene            1427..>2053
                     /locus_tag="ENO07_08235"
     CDS             1427..>2053
                     /locus_tag="ENO07_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01992.1"
                     /translation="MRKLGLGLLLLSLLFACGQEEAAEGGDDLTLNSVIAINDLPSVG
                     PVSVKQTLMFSDDVMRSQVSTSISIAGEAQEILTVYIINLNEGNIYYVNDSDSTYVGM
                     SFAEFDALLDEANPASDTAGAPLELLEDQVRRKESEARTISPFGECVPVEFDLVLGST
                     GENADYRSRMQGQMWFSNSIENAEVFKDFQEKAADRFKNAIAGNATFFG"
BASE COUNT          548 a          454 c          518 g          533 t
ORIGIN      
        1 agggataaaa aacgaatggc tcgaaatgcg tttgcagtac aggatcgctg gcgggatgtg
       61 taccatagcc atgaacccag ctgaaactgc ctggaccgcc ggtacaggag aactcgaatc
      121 ccaggctggc gccctggata ttgtagtcat tttccaccag aaactccagg gtattctctc
      181 ggccgatata aagcttattc ctggttccgc cgccgttgaa acggacaatc acattgccgt
      241 cggccatgcg cccctcgcgc ttctcggcaa tcaactgatc aatcgcttcc tccagcgaga
      301 ctcctgttat ttcctcgtaa actgcccgaa gggcatggtc agcctttcga taattgttct
      361 tctgtgcaga aagcacagaa accgtcaggc atatcagcgc aatcgacatg attaagattt
      421 tgcgccgcat agtctttcct ttctctttat cttccggaaa cagcagtcac caacaataca
      481 ttccagccac agaattattc ccaggcacct atcttaagaa tgccggcatc aatagttatt
      541 aatctttgtg ctttccaatg tcaaataata tttcaaccat aatgagattc agcctggcta
      601 atattgattt gtgagggggg atttttcgct tgatttttaa aaacttatgg gatttattac
      661 agggtcaagc aatggtattg taatggccct gacgccatat atgcgcgggc ctcagaagtt
      721 tctgaatttt ccgctatgag tctgaataat atgagattaa cagaattttc agcaaagttt
      781 tatgtactta ccctcgaata ctcaccgcat atttagaagg agataagcat ggctgaacca
      841 gattacggcg caaagcaaag cgcatccggc gtattccaga cggagtttga gttcactctt
      901 cccaagggat atgttgacaa ggatggcaat gtacataaga agggaatcat gcgtctggca
      961 aatgcgaaag atgaaatagc ccccctatcc gattatcgtg tccagcaaaa ccgggcctac
     1021 ctggtaataa ttatcctctc gcgtgtgata acgaagctcg gaaatctgtc ggatatcaat
     1081 ccgggggtta tcgagaacct gttttcggcc gatctttcat atttgcagga cttttatcgc
     1141 aagatcaacg aggagggaac ggcgaccatc gccacagtct gcccggcctg cgggcatcaa
     1201 tatgaagtcg atatgggtag cctgggaaaa tagtgagcta cccccgggac gacctgatcc
     1261 gggaggtagc ttatgtcgca tatcatttcc attggagtct tgacgagatc atggaactgg
     1321 atcatttcac caggcatcat tttatagagg agatttcgga tattaaccga aagataaata
     1381 aagggaagga agagtattag tgtttaaatc acaggagggt gaaattttga gaaaattagg
     1441 attgggtctt ctgctattat cgttattatt tgcctgcggc caggaagagg cggccgaagg
     1501 aggcgatgat ctgactttga atagtgttat tgctatcaac gacttacctt ctgtcgggcc
     1561 tgtcagtgtc aagcagactc tgatgttttc tgatgatgtg atgcgctcgc aggtgagtac
     1621 ctcgatcagt atcgccgggg aggcgcagga aatattgaca gtgtacatta taaatctcaa
     1681 cgagggcaac atctactacg tgaacgacag cgattccact tatgtcggga tgagctttgc
     1741 tgagttcgat gcgctgctgg atgaggcgaa tcccgcaagt gataccgcag gggcgccact
     1801 ggagctgctg gaagatcagg tcaggcgcaa ggaatctgaa gccaggacta tttctccttt
     1861 cggtgaatgt gtgccggttg aatttgattt agtcctgggc agtaccgggg aaaatgctga
     1921 ttatcgcagc aggatgcagg ggcagatgtg gttcagcaat tcgatagaaa atgccgaggt
     1981 cttcaaggac tttcaggaaa aggcggcgga caggttcaaa aacgctattg ccggcaatgc
     2041 cacattcttc ggg
//
LOCUS       DSEB01000464            3168 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003668, whole genome shotgun sequence.
ACCESSION   DSEB01000464 DSEB01000000
VERSION     DSEB01000464.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003668"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1326)
                     /locus_tag="ENO07_08240"
     CDS             complement(<1..1326)
                     /locus_tag="ENO07_08240"
                     /inference="COORDINATES: protein
                     motif:HMM:PF01839.21,HMM:PF13517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS01993.1"
                     /translation="MNSIFCILGILQRASIIIFISCFFLQIETHAIGLDLLFIDEGET
                     YSDNLGIMVYSPGDLDSDGYSEAFASNALGEIKVYYGGNPADTIPDHIYLCDYGVFHW
                     LSDINGDTFLDLAILRNRSGIGYFDIYFGMQEFYENAQVGLSISGDLLDGFGVGIYPQ
                     DYNGDGKIDIIINAQNAEPYAGKFYLYDGNNELDSVCDDTISICRYGVDIFTIGACVG
                     DINCDGYADYVISPDGNAMPGYTLILWGSEDPDSVPDLQIDSPFDDAIGIGYFSAGVI
                     PLGDINKDNIDDFIVTSEGFPPCIFYGGNPFDPTPKILEYPGKVANVCGDINHDGWED
                     IAVGFVSYSFGSGEVLVYFGAYDMDTIADIIMPYNSVSPSSYNFGRSVGPAGDFNGDG
                     VDDLAVGAKSYNTQNSSGYLYVFAGDPDLPTPAEDEPDIPIPEDHNILKQ"
     gene            complement(1393..1466)
                     /locus_tag="ENO07_08245"
     tRNA            complement(1393..1466)
                     /locus_tag="ENO07_08245"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:complement(1430..1432),aa:Arg,seq:cct)
     gene            complement(1793..2323)
                     /locus_tag="ENO07_08250"
     CDS             complement(1793..2323)
                     /locus_tag="ENO07_08250"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDS01994.1"
                     /translation="MNIDDLKQKIRNIPNFPKPGIVFRDITTLLDDVEAFRKVIDIYA
                     DHFQDKNINKIVAIESRGFIFGGALAYKMDAAFVPVRKLGKLPGETIKKEYELEYGVD
                     ALEMHRDAIRAGDNVLIVDDLMATGGTLAATCGMVEKLGANIAGIAVLIELSFLKGQD
                     KFQKYDYLPLIKYDSE"
     gene            complement(2325..2630)
                     /locus_tag="ENO07_08255"
     CDS             complement(2325..2630)
                     /locus_tag="ENO07_08255"
                     /inference="COORDINATES: protein motif:HMM:PF00708.16"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDS01995.1"
                     /translation="MSAQEGSAVNGKVAAKVLITGQVQGVGFRYFTTDLARSYGVNGW
                     VKNLATGDVEVKVEGDKGAVSGFLKELKIGPRHGHISNFQIEWKPYEGKFDSFQVRF"
     gene            complement(2603..3073)
                     /locus_tag="ENO07_08260"
     CDS             complement(2603..3073)
                     /locus_tag="ENO07_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HDS01996.1"
                     /translation="MSDSKKKIYVGVIGAGHCTQKLRKLSAEVGEEIAKAGAILVCGG
                     LDGVMKGAAQGAKSEGGTTIGILPGTDKSEANEFIDYVVCSGFGEARNLIIIRTADVV
                     IALPGMFGTLSELGFALKLKKPVVSLGSWDIDDKVIKAENAKEAVKLALKKVRQ"
BASE COUNT          903 a          821 c          597 g          847 t
ORIGIN      
        1 ctgttttaga atattgtggt cttcaggaat tggtatatcc ggctcatcct ctgcaggtgt
       61 gggtaagtca ggatcaccag caaaaacata taaatatcca ctactgtttt gcgtattata
      121 tgattttgcc ccaaccgcca gatcatctac tccatcacca ttgaaatccc cggccggacc
      181 aacggagcga ccaaaattat aggatgaggg agaaacagaa ttatatggca taattatatc
      241 agcaatcgtg tccatatcat aagcgccaaa atatacaaga acctcccccg atccgaatga
      301 gtacgaaaca aaaccaaccg caatatcctc ccagccatcg tgatttatgt caccacagac
      361 attagccact tttccaggat attccagtat tttaggtgtt gggtcaaatg gatttcctcc
      421 atagaagata caaggcggaa aaccttcaga ggttacaatg aagtcatcga tgttatcttt
      481 gttaatatct cctaatggga ttactcctgc agaaaaataa cctattccta ttgcatcatc
      541 aaatggagaa tcaatctgca gatccgggac tgaatcagga tcttcgctac cccataatat
      601 taacgtataa ccaggcatgg catttccatc aggagaaatt acataatctg catatccgtc
      661 acagtttatg tctcctacgc acgctccaat tgtgaatatg tcgacgccgt atcgacaaat
      721 agaaattgta tcgtcacaaa cggaatcaag ctcattgttg ccgtcatata aataaaactt
      781 gcctgcatat ggctcagcat tctgtgcatt tattatgatg tcaattttcc cgtcaccgtt
      841 atagtcttgc ggatatatgc caacgccgaa tccatccaaa agatcaccag atatacttaa
      901 accaacctgg gcattttcat aaaattcctg catcccaaaa taaatatcaa aatatccaat
      961 gcctgaccta tttcgcaata ttgccaaatc caaaaaagta tcaccattta tgtctgatag
     1021 ccaatgaaat accccatagt cgcacaaata gatatggtcg ggaatcgtat ctgcaggatt
     1081 acctccatag tagaccttga tttctcccag cgcgttcgag gcaaaagctt ccgaataccc
     1141 atccgaatcg agatctcctg gagaatatac cataatcccc aaattatccg aataagtctc
     1201 accttcatct ataaaaagaa gatccagacc tatagcatgg gtttcaatct gcaaaaaaaa
     1261 acaagaaata aaaattatta tcgatgctct ctgtaagatt cctaaaatac aaaatataga
     1321 attcaatgtt cagcatccgg cttggtagtt catttacaag ataaggctct ggagaatttc
     1381 gtcaataaaa aatacccccg gcgcgactcg aacgcgcgac acatggttta ggaaaccatt
     1441 gctctatcca cctgagctac gggggcattt tcgtcatgca cgaggacgta ctccatcagg
     1501 agccggacag accgcgcaca aatttatata tccgatatta tcaattctta tacgcaatgt
     1561 caaattattt gatgaacgag atgcgaacaa aaatccgctt tcttgtttgc cgtcaggaac
     1621 ccgactgcac atgaatgtag gtaaattcct gaagggaatt gacaatgctc tccaaataca
     1681 acgtgccaag ccccttcagg gcttgacatg cattaattgc gataatctat ggcaacaaaa
     1741 acaggcatga tcgcggggat catgccctac aaattttgag tataaattct gattattccg
     1801 aatcgtattt gatcaacggc aggtagtcgt atttctggaa tttgtcctgg cccttgagga
     1861 atgagagctc gattaaaacc gcgattccgg ctatgttggc gcccagcttc tcgaccatac
     1921 cacatgtcgc cgccagagtt ccgccggtgg ccataagatc atcgacaatc aaaacattgt
     1981 cacctgccct gatagcatcc cggtgcatct ccagagcatc gacaccatac tccagctcat
     2041 actctttctt gatcgtctcg cccggcagct tccccagctt cctgaccggc acgaaggccg
     2101 catccatttt gtaggccagc gcaccgccaa agatgaatcc ccgcgattca atggccacta
     2161 tcttgttgat atttttgtcc tggaaatgat cggcatagat atcgatcacc ttccggaacg
     2221 cctcgacatc atccagaaga gtggtaatat ccctgaagac aattcccggc ttggggaagt
     2281 tgggtatgtt cctgattttc tgctttaaat cgtcaatatt cattctaaaa tctcacttga
     2341 aaagagtcaa atttgccttc gtatggtttc cattcgatct gaaaattgga gatatggccg
     2401 tgacgcggac ctatcttcaa ctctttcagg aagccggaaa ccgcgccctt gtccccctca
     2461 accttgacct caacatcgcc ggtcgccaga tttttgaccc aaccgtttac accgtaactt
     2521 ctggccaggt ctgtggtaaa atacctgaat ccgaccccct gaacctggcc ggttatcaag
     2581 actttcgcgg ccaccttgcc gtttactgcc gaaccttctt gagcgctaat ttcaccgcct
     2641 ccttcgcatt ttcagccttg atgactttgt catcaatgtc ccagcttccc aaagatacaa
     2701 caggtttttt caatttcaaa gcaaaaccga gttccgaaag tgtcccgaac attcccggca
     2761 aggcaataac cacatccgca gttctgatta tgatcaggtt gcgggcctca ccgaatccgg
     2821 aacagacaac ataatcaata aattcattag cctctgattt gtctgtgccg ggaagtatgc
     2881 cgattgttgt gccgccctcc gattttgcgc cttgggccgc acccttcata actccatcaa
     2941 gcccgccgca aaccagaatc gccccggctt tagcaatctc ctcaccgacc tcggcggaaa
     3001 gctttcgaag tttttgcgta caatgccccg caccgattac ccccacatat atcttttttt
     3061 tggaatcgga catgtgcaga ttgactcctc taatttcctc aagatgtccc aggtgttggt
     3121 cattttttaa atcggggcga ctggatttga accagcgacc ccttagtc
//
LOCUS       DSEB01000465            4354 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001807, whole genome shotgun sequence.
ACCESSION   DSEB01000465 DSEB01000000
VERSION     DSEB01000465.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001807"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            476..1546
                     /locus_tag="ENO07_08265"
     CDS             476..1546
                     /locus_tag="ENO07_08265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDS01997.1"
                     /translation="MKKKWIAVIALLAVGTISIMSVVAMRASDQSSAAPETVKVIRRD
                     IGSAVLATGIIKPMVGAEVKVGSRISGVVKRLRANIGDYVRADQIIAELDDSELRARL
                     SQNIAAVEKARADAEFAQRQFEREYALLKKGLISDQQLDQSETALKVARALVEQAEAN
                     LQYSKVQLSYTTIRSPIAGAIASVSTQEGETVSASLAAPTFVTIIDLDRLEVQVFVDE
                     TDIGKIKIGQTARFSVDTYTDTDFRGTVTAIYPEAVIHDNVVNYIVTLTIEDLQGKTL
                     RPEMTANVTIELETRRDVLAVPTSAILRQRGERYVAVAAGGRQEQRKVTVGWRDSGYT
                     EIISGLKEGETVLIPSANQEYR"
     gene            1546..2298
                     /locus_tag="ENO07_08270"
     CDS             1546..2298
                     /locus_tag="ENO07_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDS01998.1"
                     /translation="MNKAVIQAANLTKTYNKGGGGEVHAISDISLEINRGEFVAIMGA
                     SGSGKSTLMNILGCLDRPTSGTLLLDGVEVSTLDDETLAGIRNKKIGFVFQSFNLLAR
                     TNALENVELPLIYSEKSDTRRLAREALEAVGLGGRIHHNPNELSGGQQQRVAIARALV
                     TDPEIIFADEPTGNLDSDSSLEIMALLRQLYEKGRTIVIVTHETDIAEHARRIIRISD
                     GSIVSDAKNNDPRPLDREIHKNASQDAADEDR"
     gene            2198..3502
                     /locus_tag="ENO07_08275"
     CDS             2198..3502
                     /locus_tag="ENO07_08275"
                     /inference="COORDINATES: protein motif:HMM:PF12704.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDS01999.1"
                     /translation="MVPLSVMRKTMIRGRLIEKFIKTPHRMLPMKTGRMVTMVFKSLA
                     KNRLRSFLMMIGVVIGIAAITMIVSVGLGAQQRVLDRVKKFGFESLMVRAGGGREVGR
                     PAGSQPTTSLTLEDINALKREIRAISDAAPMSVKGQMEVIYQDRSTTTGIFGVTPSWA
                     AVWDWDAADGDFISDEDMDGLARVCVLGPTVRTDLFGTLNPIGEQIRIGNVPFEVIGI
                     SQNKGISPAGGDMDNRIFVPLSTMMRRVANVDYISAAKILLGTAADIDMTVASIRSLL
                     RERHQLAPGVPDDFTITSPTEITLMAEKVSGTFNMFLALVAGISLLAGGVVVANIMLI
                     SINERRKEIGLRKAIGAKRKDIMRQFLYEATAVTLTGGVIGVILGMIGAKTLEYFTKI
                     PASISWESIAIGVFLSSLVGILAGLQPANKAASLQPIEALRG"
     gene            3510..>4354
                     /locus_tag="ENO07_08280"
     CDS             3510..>4354
                     /locus_tag="ENO07_08280"
                     /inference="COORDINATES: protein motif:HMM:PF12704.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HDS02000.1"
                     /translation="MKITRSVRISRKQLAAHPMRTVLALTGIIIGVSAVIIIVAIGNG
                     AQKEVIERIEAMGTNLLIVNAGQVQKSAGRQEIRGTVTTLTVEDAEAIAQDCSAVKFA
                     APLQSKKLQVKYENSSSNTTVVGTTPDFQEVRNFNVASGAFFTDEENTASRRIAVLGK
                     TVVDNLFYGHDPIGETIRIGKVPFEVIGVMESKGTDLSGSDQDDQIFIPIRTALRRVF
                     NLAYINAINIQAVDSKAMDHAMMQVAGLLRERHRLDRRALPDDFTIQSQTDILEAERE
                     TTDTFT"
BASE COUNT         1110 a         1091 c         1175 g          978 t
ORIGIN      
        1 atcaacatgc tcaccggtct ggctcggccg tattccgtat caatcaagat cagcggcatc
       61 ggctgcactg aaaatccttg attcggatta taatcgcaaa ccccttgcag gcgtcttctc
      121 tcgctgccga attcttccgg ataaacgggc aattgtccgg gcctgaggac agcaaaataa
      181 atgatgctta ccgctgtaat actatgcgct gctctgaaat ataccgcctc atttcttggc
      241 cgatgagaat ttgccggact atgaataaga tccggaaaaa aaaagaaaat gcactttttg
      301 agatatttgc aagttttatt caaattgagt gtccaatata gcgattgcag aaaagcagtc
      361 gaatctattg gtgccggcct cggatgtatt caagtgagcg gcatcctgct ctttatgagc
      421 agacaataga aataaatcgg gagtaacata atttgaacat aaaggaacta cagaattgaa
      481 gaaaaaatgg atagccgtca tcgctttatt agccgtcggg accatatcga ttatgtcagt
      541 ggtggccatg agagccagcg accaaagctc tgcagctcct gagacggtga aagtgatcag
      601 gcgggatatc ggctccgctg tactggcgac cggaattata aagccgatgg tcggcgccga
      661 ggtaaaggtc ggttcccgaa tatcgggcgt ggtcaaacga ctgcgcgcca atatcgggga
      721 ctatgtccgg gctgaccaga tcattgccga gctcgatgat tccgagctgc gcgcgaggct
      781 cagtcaaaat atcgccgcag tcgaaaaggc tcgtgccgac gccgagtttg cacaaaggca
      841 atttgagcgg gaatatgctc ttctaaaaaa gggtctcatc tccgatcagc agctggatca
      901 atcagaaacc gcattgaagg ttgccagagc gcttgtcgaa caggcagagg caaatctcca
      961 gtattcaaag gtccagttga gctatacgac aatccgcagc ccaatcgcgg gagcgatcgc
     1021 ctctgtatcg acacaggagg gcgaaacggt atcggcgagc cttgccgctc ccactttcgt
     1081 caccataatc gatctcgacc gactggaggt acaggtcttt gtcgatgaga ccgacatcgg
     1141 caaaataaag atcgggcaga ccgcgcgctt cagcgttgac acctataccg ataccgattt
     1201 ccggggcacc gttacagcta tttaccctga agcggttata catgataacg tggttaacta
     1261 tattgttacc ctgacaatcg aggatctgca gggcaaaacc cttcggcctg aaatgaccgc
     1321 aaacgtaacc atcgaactcg aaacccgtcg ggacgttttg gcggtgccga caagcgccat
     1381 tctgcgccag cgcggcgagc gctatgtggc cgttgctgca ggcggcagac aggaacaacg
     1441 aaaggtaacg gtcggatgga gggactctgg ctacactgaa attatcagcg gcctcaaaga
     1501 gggagagaca gttctcatac catctgctaa ccaggagtac agataatgaa taaagcagtc
     1561 attcaggcgg caaatctgac caaaacctac aacaagggcg gcggcggcga ggtacatgcc
     1621 atcagcgaca tatcgcttga gattaatcgc ggggagtttg tggccatcat gggcgcttcc
     1681 ggttccggta aatcgacgct aatgaatatc ctcggctgcc tggaccggcc caccagcggt
     1741 accctgcttc tggatggcgt tgaggtaagc acgctcgatg atgaaactct tgccggcatc
     1801 cgcaacaaga agatcggctt tgtgttccag tcctttaatt tgctggcccg caccaatgct
     1861 ctggagaacg tcgaacttcc gcttatttac tcggaaaaat cggacaccag gcgcctcgct
     1921 cgagaggcgc tggaagcagt cgggcttggg gggcgtatcc atcataatcc caatgagctc
     1981 tccgggggac agcagcagcg cgtggccatc gcccgcgcgc ttgttaccga tccagaaata
     2041 attttcgccg atgagcctac cggtaatctg gacagcgact ccagcctcga aatcatggct
     2101 ctgctccggc aattatatga aaaaggcaga accatagtca ttgtaaccca cgaaaccgat
     2161 attgccgaac acgcccggcg tatcatcaga atatcggatg gttccattgt cagtgatgcg
     2221 aaaaacaatg atccgcggcc gcttgatcga gaaattcata aaaacgcctc acaggatgct
     2281 gccgatgaag acaggtagaa tggtcactat ggtctttaag agccttgcaa agaacaggct
     2341 caggtcattt ctaatgatga tcggcgttgt aattggtatc gcagctatta ccatgattgt
     2401 ctctgtcgga ctcggcgccc aacaacgtgt tctggatcgt gtcaagaaat tcggttttga
     2461 gagtctgatg gtgcgggcgg gaggcggacg tgaggtgggc cggccggccg gttctcagcc
     2521 gacaaccagt cttacgcttg aggacattaa tgctctgaaa agagaaatac gcgcaatctc
     2581 ggatgccgct ccgatgagcg tgaaaggcca gatggaagta atttatcagg acagatcgac
     2641 caccaccgga attttcggcg taaccccgtc atgggccgct gtatgggatt gggatgccgc
     2701 agatggtgat tttatctcag atgaggatat ggatggtctt gctcgcgtat gtgttctcgg
     2761 gccgacagtg cgaaccgacc ttttcggaac gctgaacccc atcggggagc agatccgcat
     2821 tggcaatgta ccattcgaag tcatcggtat atcccagaat aagggcatca gtccggccgg
     2881 cggagacatg gacaatcgta ttttcgtccc gctgagcacc atgatgcggc gtgtcgcgaa
     2941 tgtggattat atttccgccg ccaaaatcct tcttggcacg gccgccgata ttgatatgac
     3001 agtggccagt attcggtcgc ttctccgtga acggcaccaa cttgctcccg gcgtgcccga
     3061 cgatttcacg attacaagtc cgacagagat taccctgatg gccgagaagg tctccggcac
     3121 cttcaatatg tttttggcgc tggttgcggg catttcgctg ctggccgggg gtgtggtcgt
     3181 cgctaacatc atgctcattt cgatcaatga acgccgaaag gaaatcggtt tgcgcaaagc
     3241 gatcggtgca aagcgcaaag acatcatgcg gcagtttctg tatgaggcga ctgctgtgac
     3301 tttaacaggg ggtgtaatag gcgtcatact gggaatgatt ggcgcaaaaa cactggaata
     3361 ttttactaaa atacctgcct ctatctcctg ggaaagcatc gctatcggcg tttttctttc
     3421 gagcctggtc gggattctgg ctggattgca gccggcaaat aaagctgctt cacttcagcc
     3481 aatcgaggca ttgagaggct gaccataccg tgaagatcac tcgaagcgtc cgtatttcac
     3541 gcaaacagct tgcggcacat cccatgcgaa cggtgcttgc cctgaccgga ataatcatcg
     3601 gcgtttccgc tgttatcatc attgtcgcta tcggcaatgg cgcccagaaa gaagttattg
     3661 agagaattga ggcgatggga accaatctgc tgattgtaaa tgccggacag gtacagaaga
     3721 gcgcgggacg ccaggagatt cgggggactg tcaccacgtt gaccgtcgaa gacgccgagg
     3781 cgatcgcaca ggactgttca gccgtaaaat tcgccgcacc cctgcaaagc aaaaagctgc
     3841 aggttaagta cgaaaattcg agctcgaaca ctactgtggt tggaaccacc ccggattttc
     3901 aggaggtgcg gaactttaat gtcgcttcgg gggcattttt taccgacgaa gagaataccg
     3961 cctctcgccg aatagctgtt ctgggcaaaa ctgtggtcga caatctgttt tatggacatg
     4021 atccgatcgg tgagacaatt cgcattggga aagtgccctt cgaagttatc ggcgtaatgg
     4081 aatcgaaagg gaccgaccta agcggctccg accaggacga ccagatattc atccctatcc
     4141 gaaccgcttt acggcgtgtg tttaatcttg cctatatcaa cgcaataaac atccaggctg
     4201 tggattccaa agcgatggat catgccatga tgcaggtcgc cgggttgctg cgcgaaagac
     4261 atcggctcga caggcgcgct ttgcccgatg atttcacgat tcagagtcaa acggatattc
     4321 tcgaagcaga gcgggagacc accgacacct ttac
//
LOCUS       DSEB01000466            3898 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002295, whole genome shotgun sequence.
ACCESSION   DSEB01000466 DSEB01000000
VERSION     DSEB01000466.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002295"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..733)
                     /locus_tag="ENO07_08285"
     CDS             complement(<1..733)
                     /locus_tag="ENO07_08285"
                     /inference="COORDINATES: protein motif:HMM:PF00535.24"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDS02001.1"
                     /translation="MPGRISVVVPAYNEAGNIPILVEKLDEMFRQSRLRGEVILVNDG
                     STDETQQQAQQAASKYRWLRLVNHKTNRGMTAALETGFSLARGRTLILWPADLQYMPD
                     DIPKMVAKIEDGYDVVCGWKQGKYGLKRVASFFYNALSRWIFKVKVHDLNSVKAFRKE
                     VVEDMPHLREGWHRYLVVLAAQRGYKIGEVKVKLYPRKHGKSKFGFWRIPSGISDLFS
                     VKFQMSFLRKPLLFFGSIGFILCFLG"
     gene            complement(920..2575)
                     /locus_tag="ENO07_08290"
     CDS             complement(920..2575)
                     /locus_tag="ENO07_08290"
                     /inference="COORDINATES: protein motif:HMM:PF00005.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDS02002.1"
                     /translation="MISLRNVAYQVEPDDEAPVKILTDINLEIAGGEKLAVMGANGSG
                     KTTIARLIAGLLYPTSGEVRVGGHLTSEDGFRQNLGRRAGFLFQNPDHQIVSVNVERE
                     LALGLENYGYDGRDISTRVEDYIKKYRLENVLKTPPHKLSGGEKRKLGLGSVMILEPN
                     ILILDEPMAHLDKIGKDIFKNELSKLLEDEAMTLIYITQSIEEVTGLDRLIIMDGGGI
                     ARACTISEGLNDRDALIRYGIGYPLWMDIENQDAIKRARLEAATEAREEIRSADAHTG
                     VTLECREISFGWKGQKEIIEELNLDLGKGKIHGLLGRTGCGKTTLALILAGLVKPDQG
                     RIKLNGSKASLKDLIRSVAYIFQNPERGMFAETVFDDIAYGPKNFGYKGAMLDDAVRR
                     SLEMVGLDFDRFKDRSPHTLSGGEARLAAIAGGLAVEKDIVIMDEPTEELDFKGQNRI
                     KQIVKQLARDGKSVLLISHDSDFLFEVCDYLSLCLNLSAQCFNKFELYDKPDLFPESQ
                     VEVPKILEFAYNLGLSSEFRSAGIDTFEDLNFLMPRENDSGLI"
     gene            complement(2659..>3898)
                     /locus_tag="ENO07_08295"
     CDS             complement(2659..>3898)
                     /locus_tag="ENO07_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014643734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine dehydrogenase subunit 2"
                     /protein_id="HDS02003.1"
                     /translation="FYPLGSCTMKYNPKVNEQVARFSGFSEIHPFQMTGTVQGALHLM
                     YDLAEMLKEIAGMHAVSLQPAAGAQGEFCGLLMTRKYHIKKGNDKETVIIPDSAHGTN
                     PASVTFAGYRPVQINSSADGIVSADELERVIDDKTAAIMLTNPNTLGLFESDIEKIYK
                     ICRKHDTLMYMDGANLNALLGIARPGDMGFDCVHFNLHKTFSTPHGGGGPGAGAVGVS
                     ERLEPFLPKPIIAKNEDEEYYLSYNRPDSIGQIHSFYGNFAVMVRAYAYILSNGSDGL
                     REVAEAAIINANYLKEKLKDTYHLPFDRHCMHEFVLAGTNQKKKGVKTLEIAKRILDF
                     GIHAPTIYFPLIVSEALMIEPTETENVQALDNFADILIQIDKEIDEKPEFVKESPHNT
                     PVGRLDEVLAAKRGDVAYIE"
BASE COUNT          928 a         1077 c          840 g         1053 t
ORIGIN      
        1 gccccagaaa gcaaagtatg aatcctatgg atccgaagaa taaaagcggc ttgcggagaa
       61 atgacatctg gaatttgacc gaaaatagat cggagatacc ggacggtatc ctccagaagc
      121 cgaacttgga tttgccgtgt ttgcgcggat acagcttgac cttcacctcg cctattttat
      181 agcctctctg agctgccagc accacgagat atcggtgcca gccttcacgc agatggggca
      241 tatcctccac aacctctttc ctgaaggcct tgaccgaatt caggtcgtga accttcacct
      301 tgaaaatcca ccttgataaa gcgttgtaga aaaaagaggc gacgcgtttg agcccgtatt
      361 tgccctgctt ccagccgcag acgacatcat agccgtcttc gatctttgcg accatcttgg
      421 gaatatcatc gggcatgtac tgcaggtcgg ccggccataa aatcaatgtt ctgccgcggg
      481 ccaggctgaa tccggtttcc agcgcggcgg tcattccgcg attggtcttg tggttgacca
      541 gcctcagcca gcgatatttt gaggcggcct gctgtgcctg ctgctgggtc tcatcggtag
      601 agccatcatt gaccagtatt acttcccccc tgagacggct ctgtctgaac atttcatcta
      661 atttctctac cagaatgggg atattcccgg cttcgttata ggccggcacc acgaccgata
      721 tacgaccggg catctttctt ctccggtagc ctctgctctg tccacctggg atttttgtat
      781 cctcctgcaa tcagttaagc cagttatatt atcgaaacat tgtagcccaa acagaactac
      841 tatctccaaa ttatctgaaa ataatttggc tgtataagat atctataatt atgcctttgt
      901 caagcagcct gtttttagat tatatcaagc cggagtcatt ttccctcggc ataaggaaat
      961 tcagatcctc aaaggtatct ataccggccg acctgaattc gcttgaaagg cccagattat
     1021 aagcaaattc caggatcttc ggtacttcca cctgcgattc ggggaataaa tcgggtttat
     1081 catacaactc aaatttatta aagcactgcg ccgacaaatt cagacacagg gataaataat
     1141 cgcaaacctc aaacagaaaa tcggaatcat gggagatcag gagaacagat ttgccatccc
     1201 tggccaattg tttgactatc tgctttattc tgttttgccc tttaaaatca agctcctcgg
     1261 taggctcatc cataataact atatctttct ctacggccag cccgcccgcg attgcggcaa
     1321 gcctggcctc accgccggac agcgtatgcg gtgaacggtc cttgaatctg tcaaaatcaa
     1381 gacccaccat ctcaagcgat cttctgacag catcatcgag catggcgcct ttatacccga
     1441 aatttttcgg gccataggcg atgtcatcaa aaacagtctc cgcgaacatc ccgcgttcag
     1501 gattctggaa tatgtatgca accgacctaa tcaaatcttt cagcgatgcc ttactaccat
     1561 tcagcttaat ccggccctga tccggtttaa ccagcccggc cagtatcaat gccagcgtgg
     1621 tcttgccgca tcccgttcgc cccagcaggc catggatttt gcctttcccc aaatccagat
     1681 ttagctcctc tattatttct ttttgaccct tccatccaaa gctgatctcc cggcattcca
     1741 gtgtcacgcc ggtatgagcg tcagcgcttc tgatttcttc tcttgcttct gtggccgctt
     1801 caagccgggc tctctttatt gcatcctgat tttcaatatc catccacagc gggtatccga
     1861 tgccataacg gattagcgca tctctatcgt tcaggccctc tgaaattgtg caagccctgg
     1921 ctatccctcc tccatccatg ataatcaatc tgtcaaggcc cgttacctcc tcgatgctct
     1981 gagtaatata tatcagcgtc attgcttcat cctcaagcag cttactcagc tcatttttga
     2041 aaatatcttt gccgatctta tccaggtgcg ccatcggctc atcgaggatg aggatattcg
     2101 gttcaagtat catcactgac cctaagccca gcttcctctt ttcaccgccc gacaatttat
     2161 ggggcggagt tttcagcaca ttctccagtc gatatttttt gatatagtct tccacccttg
     2221 tcgaaatatc cctgccgtca tatccataat tctcgagtcc cagcgccagc tctctttcaa
     2281 cattgactga gactatctga tgatcagggt tttggaacag aaatcccgcc cgcctgccga
     2341 gattttgcct gaaaccatct tcagaggtca gatgaccgcc caccctgact tcgccactgg
     2401 ttggatacag caatcccgca atcagccgtg caatcgttgt cttgccggag ccattggcgc
     2461 ccattaccgc cagcttttca cctccggcta tttccaggtt tatatcagta aggattttta
     2521 ctggagcctc atcatcaggt tcgacttgat atgctacatt gcgcagagaa atcatggtgt
     2581 caatttagca agatattgca aaatccaaaa tgaaaaaagg cgctcgatga gcgcctttat
     2641 ttcatagcga atcatttttt actcaatata agccacatcg ccgcgcttcg ctgccaggac
     2701 ctcatccaag cgtcccaccg gagtgttatg cggagattct ttcacaaact cgggtttctc
     2761 gtcaatctct ttgtcgatct gaatcagtat atctgcaaaa ttgtcgagcg cctgcacatt
     2821 ttctgtctcg gtcggctcga tcattaaagc ctcggacact atcaggggaa agtagatcgt
     2881 gggcgcatgg atcccgaaat ccagaatccg cttggcaatc tcgagtgttt tcacgccttt
     2941 cttcttctga tttgtgccgg ccagcacgaa ctcatgcata caatgccggt cgaatggcag
     3001 atggtatgta tctttcagtt tctctttcaa atagttggcg ttgataatag ccgcctcagc
     3061 cacttcacgc aaaccgtccg agccattgct caaaatataa gcataagccc ggaccatgac
     3121 cgcaaaatta ccgtagaagc tgtgaatctg cccgatcgaa tccgggcgat tgtagctgag
     3181 ataatactcc tcatcttcat tcttcgcaat aatcggcttg ggcaaaaatg gctccagtct
     3241 ttcgctcacg ccgaccgcac cggcgcccgg accgccgccc ccgtgggggg tcgagaatgt
     3301 cttgtgcaga ttgaaatgca cacagtcgaa gcccatatca cccggacgcg cgatgcccag
     3361 cagcgcattc agattggcgc catccatgta catcagggta tcatgtttgc ggcagatctt
     3421 atatattttc tcaatatccg attcaaacag tccaagtgta ttcggattgg tcagcataat
     3481 cgcggccgtt ttatcgtcta ttactctttc cagttcatca gcagagacta tcccatctgc
     3541 gctggaatta atctggaccg gtctgtagcc cgcaaatgtt accgaggccg gattggtgcc
     3601 gtgagccgaa tcagggataa ttaccgtttc cttgtcattc ccctttttga tatgatactt
     3661 gcgggtcatc agaagcccgc agaattcacc ctgggcaccc gcggcgggct gcagtgatac
     3721 tgcatgcatg cccgcgattt ctttcagcat ctcggccagg tcatacatca gatgcagggc
     3781 gccttgcaca gttccggtca tctggaatgg atgaatctcg gagaatccgg aaaagcgcgc
     3841 cacctgctcg ttgaccttgg gattatactt catcgtgcat gatcccagcg gatagaaa
//
LOCUS       DSEB01000467            4039 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002130, whole genome shotgun sequence.
ACCESSION   DSEB01000467 DSEB01000000
VERSION     DSEB01000467.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002130"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1361
                     /locus_tag="ENO07_08300"
     CDS             <1..1361
                     /locus_tag="ENO07_08300"
                     /inference="COORDINATES: protein motif:HMM:PF13174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDS02004.1"
                     /translation="MRIGMVHTFQGRLEQGRRYLALAQERSENLPVRERMLLDIYSDL
                     WLYTNIDDAFVKIKSYVKNYPDDWEGRFFYAIFLYQLGEKFDAALAQIDTMLLINPRS
                     RWGLSLAATIHASQDDLDKAIEKVKLVKSYFPDSPQPYLELSNLYRQTKSYDLALSEI
                     SQLLEIYPDNYDGIIRAARINILQRDFEKARENLELLKKYYPDNPHIMNEYEDLQIDL
                     EVWEGNFLDAVNRYYKLIDYALKTEDRTIIQTAYSSFANYFLNFDYPDSVVKYAEKAH
                     EYAQLFENLDYALTLVELDTSYCAGAEKVLQEVLKEFRERMPENIWPIANYLEDIFYG
                     TCNLDTLKVIEAYDTLFNSPLNMSVSSTAFALGSLMVSYGMYEEGLKYLERITTGDLG
                     TTAPLRYLKAIYYIGVAQQELGNYSEARRNFKELLKHWGDPQIETEKIRDARKRLQEL
                     PS"
     gene            complement(1490..2701)
                     /locus_tag="ENO07_08305"
     CDS             complement(1490..2701)
                     /locus_tag="ENO07_08305"
                     /inference="COORDINATES: protein motif:HMM:PF02687.19"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDS02005.1"
                     /translation="MQFLESFRVAANSLRTHKVRSFLTMLGIIIGVASVITVFAMGRG
                     AERATKEQIQKYGTNVLTLYPEYGRSHSARASGQRVRLYNEDVEPLQEIPGIVAAVPQ
                     MYNFTQVKFGNEVMYARILGTTPEYEWVNNSPLVRGKYFSRTDNASRRRVAIIGTEIR
                     KQLFGEDTLSVVGSEIKIRGISFEVIGELKEKGPGWSSPDEMVVIPLLTAQKRVFGYN
                     YLNAITVKVSSLDLMDPVSLAIERALRRQHGLTGNDENDFRIFSQVDIIQTYQSTTDT
                     FNWLLGSIAGVSLLVGGIGIMNIMLVSVTERTREIGLRMAVGARKRDIRLQFLIEAFA
                     LSIFGGALGIFLGSGASKILSTYFNWNTLIAPDSIMLAFGFAAFVGIVFGFYPALKAS
                     KLDPIESLRYE"
     gene            complement(3109..3618)
                     /locus_tag="ENO07_08310"
     CDS             complement(3109..3618)
                     /locus_tag="ENO07_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS02006.1"
                     /translation="MFKKFIIAIMAVVMMYSIAVAQEASTASFGFRGWGPRIGVTIDP
                     DQFHFGGHADFGHFAKHIRLQPNLEIGFGDNLMLLAINFEGSYRFATNWDVWTPYIGA
                     GPGINFYNFDDEGLPDGADDSETEFGFNILGGIEKGISNGDRFFLELKLGFADSPDIK
                     ITAGWTFFH"
     gene            complement(3643..3933)
                     /locus_tag="ENO07_08315"
     CDS             complement(3643..3933)
                     /locus_tag="ENO07_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS02007.1"
                     /translation="MPDGEEYSFETHDVTLRTESDTRGDVEKIAAGALAGAVIGAIAK
                     GDEGAAVGAVVGAAAGGAVVIATQENRITLKPGQKFLIQTTQPVKLPAPQAD"
BASE COUNT         1016 a         1011 c          988 g         1024 t
ORIGIN      
        1 atatgcgcat aggcatggtg cacaccttcc agggccgtct ggaacagggc aggcggtatc
       61 tggcgctggc ccaggaacgt tccgaaaatc tcccggtaag agagcggatg cttctggata
      121 tttactctga tttgtggctt tataccaata ttgatgatgc ttttgttaag ataaagtcgt
      181 atgtcaagaa ttatcctgat gactgggagg gcaggttttt ctatgccata ttcttatatc
      241 agctgggaga gaagtttgat gcagcgctgg cccagatcga cacaatgctg ttgattaatc
      301 cgcgcagcag atggggattg agcctggccg ccacgattca cgccagccag gatgaccttg
      361 acaaagcaat agagaaggtg aaactggtca agtcatactt tcccgattcc ccccagccgt
      421 atctggagct ctcgaatctg tatcggcaga cgaaaagtta tgatctggca ctgtctgaaa
      481 tttcgcagct tctggagata tatcccgata actatgatgg aattatcagg gcggccagga
      541 taaatatatt acagcgggat tttgagaagg cccgcgagaa tctggagctt ttaaagaaat
      601 attaccctga caatccgcat atcatgaatg aatatgagga cttgcagatc gatctcgagg
      661 tctgggaagg aaattttctg gatgcggtga atcgctatta caagctgatc gattacgctt
      721 tgaaaactga ggaccggacg attatccaga ccgcatacag ttctttcgca aattactttc
      781 tcaacttcga ttaccctgac agcgtcgtga aatatgcgga gaaagcgcat gagtatgccc
      841 agcttttcga aaatcttgac tacgccctga ccctggttga gctcgacact tcatactgcg
      901 ccggagccga gaaggtgctt caggaagtat tgaaggaatt cagggaaaga atgcccgaaa
      961 atatctggcc gattgcgaat tacttagagg atattttcta cggaacctgt aatctggata
     1021 ccttgaaggt tatcgaggct tacgatacac ttttcaattc gcctctgaac atgagtgtgt
     1081 catcgaccgc ttttgctctg ggaagcctga tggtttctta cggtatgtac gaggaaggtc
     1141 tgaaatattt agagagaatc actaccggcg atcttggtac cacagcccca ttaagatacc
     1201 tgaaggcaat ctattatatc ggagtggctc agcaggaact gggcaactac tcagaagcac
     1261 gcagaaattt caaggagctg ctgaaacact ggggtgatcc gcagatcgag actgagaaga
     1321 tcagggacgc acgcaaaagg ttgcaggagc tgccttcttg attagcctcg tattgaccat
     1381 ctgaattcca gacctctgca ggctgaataa cctctccatc tgatgagact tttcgtccgc
     1441 caaatgccgc atccatggct tcgctcagaa tgactaagat tgggtaggac tattcgtagc
     1501 gtaatgactc gatcgggtcg agtttggagg ctttcaaggc gggatagaac ccgaaaacta
     1561 tgcccacaaa ggccgcaaat ccgaaggcca gcatgatcga gtccggtgct atcagtgtat
     1621 tccagttgaa gtatgtggag agtattttgg atgcccccga tccgaggaat attcccagtg
     1681 cgccgccgaa gattgaaagc gcaaacgcct ctatcaggaa ctgcaggcgg atatcccgct
     1741 tccgcgctcc caccgccata cgcagaccta tttcgcgcgt gcgttcggtg accgacacca
     1801 gcatgatatt cataataccg ataccgccta ccagaagcga aactccggcg atcgatccca
     1861 gcagccagtt gaaggtgtcg gttgtgctct ggtaggtctg gattatatcc acctgactga
     1921 agattctaaa gtcgttttca tcattgccgg tcaatccgtg ttgtcgcctg agggcccttt
     1981 caatcgccag agaaaccgga tccatcaaat ccaggcttga gacctttact gtgatggcgt
     2041 taaggtaatt atatccgaag acgcgtttct gggcggtcag gagcggaatg acgaccatct
     2101 catcaggcga ggaccatccc ggccctttct ccttaagctc tccgattacc tcgaagctga
     2161 tccctctgat tttgatttcg cttccgacaa cagataaagt gtcctcaccg aaaagctgtt
     2221 ttcttatttc tgtaccgatt atagccactc gcctgcggct ggcattatct gttcgagaga
     2281 agtatttgcc gcgcaccagg ggcgagttgt tgacccactc gtattcgggg gtagtgccca
     2341 ggatgcgagc atacatgacc tcattgccga atttgacctg agtgaaatta tacatctgcg
     2401 gaaccgcagc gactattccg ggaatctcct gcaaaggctc cacatcctcg ttgtatagac
     2461 gcactcgctg tccggaggcc ctggcggaat gggaacggcc gtactcggga taaagtgtca
     2521 gaacattggt gccgtatttc tgaatctgct ctttggtggc cctttccgct ccgcgcccca
     2581 tggcaaagac ggttatgacc gaggccacgc cgataatgat acccagcatg gtgagaaatg
     2641 atctgacttt atgggttcgc agcgagttag ccgcgaccct gaaagactct aaaaattgca
     2701 taactggaag atacgctcct tttgggcaat gtcaaatgaa tcctgaatac ccaattcagg
     2761 tgccccaccg cttgagagtt tgtcaataag tcacgaatat atacgtatag tcatcacgag
     2821 gcgcgaaaac tcttaagtct tagcgacgtg gtgagctcat aagttccttc atataaagca
     2881 ttttgagatt cccacggtcg tcctcgaccg aaggtcggga ctccctcgga atgacgtctc
     2941 tcagacgtac tgtcaatatc cagcactttc taaacactcc ctttagcgaa caaatttctc
     3001 agattatata tgatttgaca ccgtttatac ggctatgctt gcaaaagatt acaataaaaa
     3061 acggcggata ataatatccg ccgttcaaat taaaactgct tgccttaatc aatggaagaa
     3121 tgtccagccc gcggtgattt taatatccgg ggaatcggca aagccgagtt tcagctcaag
     3181 aaagaacctg tcgccgttgg aaatgccttt ctcgatgccg cctaatatat tgaatccgaa
     3241 ttcggtttcg ctgtcgtccg ccccatccgg caagccttca tcgtcaaaat tgtaaaaatt
     3301 gatccccggt ccggcgccta tatagggcgt ccagacatcc cagttggtgg cgaatcggta
     3361 tgatccctca aagttgattg ccagaagcat tagattatcg ccgaaaccaa tttccagatt
     3421 tggctggaga cggatgtgtt tggcaaagtg tccaaaatca gcatgtcctc caaaatgaaa
     3481 ttggtcggga tcgattgtga ccccgattct gggaccccat ccgcgaaatc caaaactcgc
     3541 tgtggaagct tcctgggcaa ctgcgataga atacatcatc acaactgcca ttatggcaat
     3601 gataaacttc ttaaacatta tatctcctct tctgttattt acttagtcgg cctgtggcgc
     3661 cggcagcttt acaggctggg ttgtctgaat caggaatttc tgtccgggct taagcgttat
     3721 tcggttctcc tgtgtggcta taaccacggc gcctccggcg gcagcgccca caacagcgcc
     3781 gaccgcggca ccttcatccc ctttcgcaat cgccccgata acagccccgg ccagcgcacc
     3841 tgcggcaatt ttctcgacgt cgccgcgggt atccgattcg gttctgagag tgacatcgtg
     3901 agtctcgaac gagtattcct caccatccgg cataacgatc gtgttgaagg ccagggtcat
     3961 ctcggcatct tcactttcgc tctctggttc atcgacctct gtcagggtgc ccctgactgt
     4021 tgttccggca ggtacgacg
//
LOCUS       DSEB01000468            3902 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002291, whole genome shotgun sequence.
ACCESSION   DSEB01000468 DSEB01000000
VERSION     DSEB01000468.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002291"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..137)
                     /locus_tag="ENO07_08320"
     CDS             complement(<1..137)
                     /locus_tag="ENO07_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDS02008.1"
                     /translation="MFHKVSAVSDVSFEVFRGELFGSLGPNGASKTTTINMLTGLARP
                     YS"
     gene            complement(529..903)
                     /locus_tag="ENO07_08325"
     CDS             complement(529..903)
                     /locus_tag="ENO07_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HDS02009.1"
                     /translation="MSEEKSEKIVIFGTHGPEDPELACLPFVIGNAALVLEVDVTIML
                     QGSAVLIAKEGCHEHVFAAGLPPLSDLIKDFIEQGGKLLICSPCIQERKITPDMLVKI
                     AQPVKAARAVMEMLEATQVLSY"
     gene            complement(984..1973)
                     /locus_tag="ENO07_08330"
     CDS             complement(984..1973)
                     /locus_tag="ENO07_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDS02010.1"
                     /translation="MSLPKKAILGILTDNLLNRGSVLPLSDRTAIQWSDGLDIPRGGE
                     TVLYTGHMYQIMPYLGTMANRMKAMENSWITKFMNLGRFVNRFINVSRFMAFPGKNEK
                     QTYNNILRAIALLLRKGGVKYGYLYEDELYSGALIYDQGVDMALANHARRVRRVFEDH
                     GVRKVITVDPHTTNMLRHVYPEILEDFDLEVQSYLEVLADADLENAEKSNGEVMIHDS
                     CVYARYEGVIEQPRYLLRRAGFTVKSPELSGALTHCCGGPIESLFPSRAHEIAAKRME
                     QLRTMGDPIAAMCPICLVNLKQAGKNNDEVGDIAEFLARALLEVPGPKERVTA"
     gene            complement(1978..2280)
                     /locus_tag="ENO07_08335"
     CDS             complement(1978..2280)
                     /locus_tag="ENO07_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013755830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDS02011.1"
                     /translation="MTTVNTVFIESIDPRFDAAACMNCGVCTALCPLEIIPLPRMLFR
                     EVMLGLEDKIIENEDTIYSCLLCKMCEANCPAGVPIAENIRLLRNYINSALYGLGR"
     gene            complement(2277..2531)
                     /locus_tag="ENO07_08340"
     CDS             complement(2277..2531)
                     /locus_tag="ENO07_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDS02012.1"
                     /translation="MMEKSEMRNLQEVLRDEMVMHERICDLLKKNPMTIPELAKALNK
                     PSWETTVWIMSMRRYGMVAELPKSRGDDYFKYTILPEESE"
     gene            complement(2528..>3902)
                     /locus_tag="ENO07_08345"
     CDS             complement(2528..>3902)
                     /locus_tag="ENO07_08345"
                     /inference="COORDINATES: protein motif:HMM:PF07992.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HDS02013.1"
                     /translation="IRLIRAGIARTLMGHPLEMIRVKTIPRVLIIGAGISGLQAARLL
                     STMGIEVHLVESAPEVGGQVASWGPLFPSGRSGKELVQTLTSMIEKDDRIHIHTSSQV
                     VEKTGNVGEFDISIQSSDGKRTSIKAGAIIVATGFQRYKPPQGEFGYGLPGVLTLDEF
                     RDVLDNSDSGSIRYNGRDIKNISYIYCVGSRQGSNDEGSHSYCSRYCCTAAVHTALLA
                     GEKQNPLHQYHLFRDMRTYGKQELLYEEALQKGSLFLKFPDEEPPSVKASNGHLEVAL
                     KDQLTGGEEINIGSDLVVLVTGMEARPNRELIDVLKLPVGKDGFFNEIHCKLRPVETV
                     MDGILIAGSAQGPKNVHESTISSMAAVAKSAGLLMKGYVDLEPFVAVVDPDLCEWCDD
                     CSNACPYDAIGKIEKGGKAIAQVTTAICKGCGTCVPACEKLAINVEGYTHKQITNMID
                     ALAKEVV"
BASE COUNT          936 a         1051 c          910 g         1005 t
ORIGIN      
        1 gaatacggcc gagccagacc ggtgagcatg ttgattgtcg tggtcttact ggcgccattg
       61 ggaccgagag agccgaacaa ttcccctctg aaaacctcga aggaaacgtc tgaaacagcg
      121 ctgactttgt ggaagattta ctcaggccct ctacggcaat agtattattc atttcttttt
      181 tcaggggtgc acgggtattt cttttagccg caatggcatt cccggatcgg ccacagtaac
      241 tcctctaatt gagtattaac gcagatactt taatacttcg atgcgacaaa gtcaacctgg
      301 atcaattgaa ctgagtttcc cgaatacccc tgaagttcaa aacagagact gcgataatac
      361 tccgataagg cagtttccaa cttctgccgt tgattctatc gtagtttaaa tcgaacagga
      421 accgtgaagc tcacaaaagt ttgggcagag tgatatcggg gccgctccgt ttcatttacc
      481 gcaatataaa attagcgtgt agtgctgaga ctgcgataag cagtccgatt agtagctcaa
      541 aacctgggtc gcctccagca tctccatgac agctctggca gctttgaccg gctgtgctat
      601 ttttaccagc atatccggcg taatctttcg ctcctgaata cacgggctgc aaatcagaag
      661 cttgccgccc tgctcaatga agtcctttat caaatccgac agaggcggca atccggcggc
      721 aaaaacatgt tcgtggcaac cctctttggc aatcaaaacc gccgatcctt gaagcatgat
      781 agtaacgtcc acctccaata caagggcggc gttgccgata acaaaaggca aacatgccaa
      841 ctcggggtct tcgggtccat gcgttccaaa gataacaatt ttctctgatt tctcttcact
      901 cattgttgtt gctcctgact catattaatt caagttgctg gccatcaggc ccaaaaccgt
      961 ttgaaaatgt tctccatgtt ttgctaagcc gtcaccctct ctttcggccc gggtacttcc
     1021 aataatgctc tggcgagaaa ttcagcgata tccccgacct catcattatt ttttccggcc
     1081 tgtttgagat tgaccaggca gatggggcac atggcggcaa taggatcccc catggtcctc
     1141 agctgctcca tccgtttagc ggcaatttca tgcgctcgag agggaaaaag ggactcaatt
     1201 ggcccgccgc agcaatgcgt cagggcgccc gaaagctcag ggcttttgac tgtaaaacct
     1261 gccctcctga gcaggtaccg cggttgttcg atgactcctt cataacgggc ataaacacat
     1321 gagtcgtgta tcatcacttc accattggat ttctctgcgt tttccagatc ggcatctgcc
     1381 agaacttcca gataactttg cacctccaga tcaaaatctt ccagaatctc gggataaaca
     1441 tgccgcagca tattggttgt atgcggatcg accgttataa ccttcctgac cccgtgatcc
     1501 tcaaaaaccc tgcggacgcg tcgggcatga tttgctaatg ccatgtccac tccctgatcg
     1561 tatatgagcg cgccggaata aagttcatcc tcgtagagat aaccgtactt gacacccccc
     1621 tttctcaaaa gcagggcaat tgcacgcagt atattgttat aggtctgctt ttcatttttc
     1681 cccggaaagg ccatgaaacg ggatacatta ataaacctgt tcacgaatct gcccagattc
     1741 atgaattttg tgatccatga gttctccatc gccttcatgc ggttggccat ggttccgagg
     1801 tagggcatta tctggtacat gtgcccggtg taaaggactg tctcgccgcc ccggggaata
     1861 tcgagcccat cggaccattg gatggctgtc ctatcggaaa gcggcagcac gctcccgcga
     1921 tttaagaggt tgtcagttaa aatgccgagt atcgctttct tgggtaaaga catgtactta
     1981 cctccccagt ccatacagag cgctattaat ataattgcgc aagaggcgaa tattctccgc
     2041 aataggaact ccggcgggac aattggcctc gcacattttg catagaagac aggagtatat
     2101 ggtatcctca ttttcgatta ttttgtcttc gagtcccagc attacctccc ggaaaagcat
     2161 gcgtggaagc gggataatct ccagaggaca gagcgcggtg caaacaccgc aattcataca
     2221 ggctgcggca tcgaagcgcg gatcgattga ttctatgaaa acagtgttga cagtggtcat
     2281 tccgactcct ccggtaaaat agtgtacttg aaataatcat caccccggct cttgggcagc
     2341 tcggcgacca tgccgtaacg ccgcatcgac atgatccaca ctgtcgtctc ccaactcggc
     2401 ttatttaagg cttttgccag ctcaggaatg gtcatgggat ttttcttcag cagatcgcag
     2461 atacgttcat gcatcaccat ctcgtcccgc agaacttcct gcagattgcg catttcactc
     2521 ttctccatca taccacctct ttggccagag catcaatcat gtttgtgatt tgcttgtggg
     2581 tataaccctc aacatttatt gccagctttt cacatgccgg cacacaggtt ccgcaaccct
     2641 tgcatatggc agtggtgacc tgcgcgattg ccttgccgcc tttttcgatt ttgccgatgg
     2701 catcgtatgg acaggcgttg ctgcaatcat cacaccattc gcataaatca ggatcaacca
     2761 cggcgacaaa cggctcgaga tcaacatagc ctttcataag cagcccggcg ctcttggcaa
     2821 cggcggccat agatgagatt gtgctttcat gcacgttctt ggggccctgc gctgaacctg
     2881 cgatcaggat cccgtccatg acagtttcca ccgggcgcaa tttgcagtgg atctcattga
     2941 aaaaaccatc cttccctacc gggagcttga gcacatcaat caactctcgg ttcggacgag
     3001 cttccattcc tgtgacaagc accaccagat ccgatccgat gttgatctcc tctcccccgg
     3061 tgagttgatc ttttaaggca acctcaagat gaccgttgga ggcttttact gaaggcggct
     3121 cctcatccgg gaatttaaga aacagcgatc ccttctgcag ggcttcttca tagagaagct
     3181 cctgtttgcc ataggtacgc atgtcccgga aaaggtgata ttgatgcagg ggattttgtt
     3241 tctctccggc aaggagcgca gtgtggacag cggcggtaca gcagtatcgg gagcagtagg
     3301 aatgagagcc ctcgtcattt gagccctgcc tgctgccgac acagtatata taggaaatat
     3361 ttttaatatc tcgtccatta taccggattg aaccgctgtc tgaattatcc aggacatccc
     3421 tgaattcgtc cagtgttaat actcccggca gcccatatcc aaattctcct tgaggcggct
     3481 tatagcgctg aaacccggtg gcaacgatga tcgcccccgc ttttatagaa gtgcgtttgc
     3541 cgtccgagct ctgaatggaa atatcgaact cgccgacatt gccggtcttc tctacaactt
     3601 gagacgatgt atgtatatga attcggtcat ctttctcgat catgcttgtg agggtctgaa
     3661 ccagctcctt tccgcttcgg ccgctgggaa aaagagggcc ccaggatgcg acctggccgc
     3721 ctacctcggg ggcgctttcc accagatgta cttcaattcc catagtcgaa agcaaccgtg
     3781 cggcctgcag gccgctgatg cccgcaccta tgatcaggac acgcggaatt gtcttgaccc
     3841 ggatcatctc caatggatgc cccatcagtg ttctggcaat gccggcccgg ataagccgga
     3901 ta
//
LOCUS       DSEB01000469            4361 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001800, whole genome shotgun sequence.
ACCESSION   DSEB01000469 DSEB01000000
VERSION     DSEB01000469.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001800"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..137)
                     /locus_tag="ENO07_08350"
     CDS             complement(<1..137)
                     /locus_tag="ENO07_08350"
                     /inference="COORDINATES: protein motif:HMM:PF00005.25"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDS02014.1"
                     /translation="MMGVLEVKGLSLTLDGNPILNKLDIDFWDGHVHAVVGPNGAGKS
                     TL"
     gene            complement(198..1370)
                     /locus_tag="ENO07_08355"
     CDS             complement(198..1370)
                     /locus_tag="ENO07_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS02015.1"
                     /translation="MELLDINELKLLANCQEQPCISIYMPTHRTGREVEQDPIRLKNL
                     LREADRKLGQYGLRRKEIDELLDPAEKLLGSSDFWNHQSDGLAVFLNKNESHIYRLPL
                     SFEEAAIVSHRHHLKRLLPLFVNDGRFFLLALSQKKSRLFEATKHSISEYELEGVPLS
                     LEEFMKYEDPEKQLQYHTGTGDAGGGGKPGKRSAMYHGHGAGMDDAAKKEYISRFFKS
                     VENGIDKIIAGRQEPLILVGYEHYIPMYREANSYDKLLDNEVRKNPDDLREKEIHELA
                     WKEVEPYFGQERKNYIKKYKEILGTGKASNNTEQIIKAAHGNRVECLFLKKGQQILGS
                     YQPEEDKIVVYEKEQPGSEDLLDFAAIRTILSGGAVYIMEADDMPDENPAAAIYRY"
     gene            complement(1411..4104)
                     /locus_tag="ENO07_08360"
     CDS             complement(1411..4104)
                     /locus_tag="ENO07_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015138022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HDS02016.1"
                     /translation="MANTKSDVRVNKDSREPWRRGAEELLRELDVDRNKGLSSSEAAE
                     RQSKFGKNLLREVEERSIWSIFADQFKSLVILILAVASALSFIFGEVIQGFAVAGAIL
                     INALIGFFTELKAVKSMQSLREMSKVTTKVRRDGEAKNINAREIVPGDIVILEGGDIV
                     TADMRLLEANKLQLNESALTGESMPVSKQVEALDQDVPLAERSNMLFKGTAVTRGSGE
                     GLVISTGMNTELGEISELVEAAGEDLTPLEKRLNRLGHSLIWVTLAVAAVVAVTGILR
                     GKELVLMIETSVALAVAAIPEGLPIVATIALARGMMRMARRNALVNRLASVETLGATN
                     VICTDKTGTLTENEMTVTKLILETGEIKITEDRFEDSNGPIDEPSEALKKALIVGVLC
                     NNASLSRGNTPDSDSHVGDPIEVAILEAADKLNYRRSELLEQMPEEREEAFDPDTKMM
                     ATFNRNNGGYMVSVKGAPESVFEVCTNVLAGDDIRNLDNADRWEKLNNEMAEDGLRVL
                     ALAFKQSESPDANPYNDLTLIGLMGFTDPPREDVKDSIKVCHKAGIRVIMVTGDQEVT
                     ARNVGEQVGLVENDNAAVMKSRELHDVESLSDKDKERIINASIFARVSPRQKLDLISI
                     HQENNSIVAMTGDGVNDAPALKKADIGIAMGRRGTQVAREAADMILKDDAFSTIVAAV
                     QQGRVIFDNIRKFVIYLLSGNSSEIIAVGAASLANAPLPLMPLQILFLNLVLDVFPAL
                     ALGVGEGDPRIMNRRPRPADEPVLGWNHWGFIGAFGLLIAASVLAAFAIAINWLKLDT
                     GQAVTVSFLTLAFARLWHVFNMRDLRSGFISNEITRNVYVWGALFLCTVLLLSVVYIP
                     GPAKVIGVVPLDLKRWALILPMSLIPWIAGQIYKSVGGREK"
     gene            complement(4114..>4361)
                     /locus_tag="ENO07_08365"
     CDS             complement(4114..>4361)
                     /locus_tag="ENO07_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS02017.1"
                     /translation="RKQIQDGENGYLVSSVDETAERILKLLKDKKLRDKMGQKARESV
                     KKKYLLSRYLENYLDLFNSYETSYTLKSHRIGNEFTD"
BASE COUNT         1015 a         1251 c          935 g         1160 t
ORIGIN      
        1 agggtcgatt ttccggcgcc gtttgggccc accacggcat gcacgtggcc atcccagaaa
       61 tcgatatcga gcttattgag gatcggattc ccgtcaagag tcaggctcaa tccttttact
      121 tcaagaactc ccatcataat cttcctgtgt tacaatttga tgcatataca aaaatgctgc
      181 attcaggttt accattctca atagcgatat atcgccgctg ccggattctc gtccggcata
      241 tcatccgctt ccattatata taccgctcca cccgataata tagtcctgat cgcggcaaaa
      301 tccaacaaat cttccgaccc cggttgttcc ttttcataca ctacaatctt atcctcctca
      361 ggctgatagc tccccagaat ctgctgtcct ttcttcaaaa acaggcattc gacacgattg
      421 ccgtgggccg cttttattat ctgttccgta ttattcgagg ccttaccggt accaaggatt
      481 tccttgtatt tcttgatata atttttgcgt tcctgaccaa aatacggctc cacctctttc
      541 caggccagct catgtatctc cttttccctc aaatcatccg ggttctttct aacttcattg
      601 tccagaagct tgtcatagga attggcctcc ctgtacatgg gaatataatg ttcataaccc
      661 accagtataa gaggttcctg acgtcccgct atgattttgt ctattccatt ctcgactgac
      721 ttgaaaaagc gagagatgta ttccttcttg gctgcatcgt ccataccggc gccatgacca
      781 tggtacatag cgctgcgctt gcccggtttg ccgccgcccc ctgcatctcc ggttccggta
      841 tgatactgca gctgtttttc cggatcctca tacttcatga actcctcaag actgagcggc
      901 acaccctcca gttcatattc actaatcgag tgttttgtgg cctcaaaaag gcggctcttt
      961 ttctggctca gggccagcag gaagaagcgg ccgtcattca caaacagcgg taagagccgc
     1021 tttagatgat gccggtggga gactatggcc gcctcctcaa aggataacgg caaccgatag
     1081 atatgcgact catttttgtt caaaaagacc gccaggccgt cgctctgatg attccagaaa
     1141 tcggaacttc ccaaaagctt ctcggcagga tcaagcaact cgtcaatttc ttttctgcgc
     1201 aaaccgtatt gccccagttt gcggtcggcc tccctcagga gatttttgag cctgatggga
     1261 tcctgctcga cctctctacc cgtgcggtgc gtgggcatat aaatcgaaat acagggctgc
     1321 tcctggcagt tggccagtaa ctttagttca tttatgtcaa gcaattccat agaaactcaa
     1381 ctcctcttat ttttgagatt ggttctgcaa ctatttttct cttcctccga ctgatttata
     1441 aatctggccc gctatccagg gaatcaaact catcggcaga atcagggccc agcgcttcaa
     1501 atccagcggt accactccaa tgaccttagc gggtcccgga atatacacta ccgataacaa
     1561 caatactgta cataaaaaca acgctcccca gacataaacg ttccttgtta tttcgttgct
     1621 gataaagccg ctgcgtaaat cgcgcatatt gaaaacatgc cacagccggg caaatgccag
     1681 tgtcagaaat gaaaccgtca ccgcctggcc ggtatcgagt ttgagccagt tgattgcaat
     1741 tgcaaatgct gccagtaccg aggcggcaat caataatccg aaggctccta taaaacccca
     1801 atgattccaa ccgagtaccg gctcatcagc aggacgcggc ctgcggttca tgatgcgcgg
     1861 atcaccttcg cccaccccca gggccagggc cggaaataca tccagcacga ggtttaaaaa
     1921 gagaatctgc agcggcatta aaggcagagg ggcatttgcc agagatgctg cccccaccgc
     1981 gattatctca ctggaattcc cggagaggag atagattaca aacttgcgta tgttgtcaaa
     2041 aataacccgg ccctgctgta ccgctgcaac tatggtcgag aaagcatcat ccttgaggat
     2101 catatccgcc gcttctcgcg caacctgggt tccccgcctg cccatggcaa taccgatatc
     2161 cgccttcttc agggcagggg catcgttgac cccgtcgccg gtcatggcca caatggaatt
     2221 gttctcctga tgaatcgaga tcaaatccag tttctggcga gggctgaccc tcgcgaatat
     2281 ggaggcattt attattcgct ctttgtcttt atccgacagg ctttcaacat catgcaattc
     2341 ccggctcttc atcacagcgg cattatcatt ctcaaccagg ccaacctgct cccccacatt
     2401 tcgggcggta acctcctgat cgccggtaac cattatgacc ctgataccgg ctttgtggca
     2461 taccttaatt gaatccttta catcctcacg gggaggatcc gtgaatccca ttagaccaat
     2521 gagggtcaaa tcgttataag gattagcatc cgggctctct gattgcttga atgccagggc
     2581 caataccctc aggccatcct ccgccatctc attattcagc ttctcccacc tgtcagcatt
     2641 atccagattt ctaatatcat caccggccag cacattggta caaacctcaa acacactttc
     2701 aggtgcgcct ttaaccgata ccatgtagcc gccgttattg cggttgaatg tagccatcat
     2761 cttggtgtcg ggatcgaaag cctcttcgcg ctcctcgggc atttgctcta ataattcgga
     2821 acgtctgtaa ttaagtttgt cggccgcctc caggatcgcc acttcgatcg gatctccgac
     2881 gtgactatca ctatcagggg tattgccccg ggaaagggag gcattattgc acagcacccc
     2941 gacaatcagc gctttcttta aagcttcaga gggttcatcg ataggtccgt tcgagtcttc
     3001 aaagcggtcc tccgtgattt taatttcccc cgtctccagt attaattttg taaccgtcat
     3061 ctcattttca gtcagcgttc cggtcttgtc ggtacagata acattggtcg ctcccagggt
     3121 ctcaaccgag gccagccggt tgaccagggc attgcgtctg gccatccgca tcataccccg
     3181 cgcgagtgcg atggtggcca caatcggaag gccctcgggg atcgctgcaa cagccagtgc
     3241 cacagaggtt tcaatcataa gtacgagttc cttaccgcgc aaaattcccg ttacagctac
     3301 aactgcggca accgccaatg ttacccagat caggctgtgt cccaggcggt tcaggcgttt
     3361 ctccagcggg gtgagatcct ctcccgcggc ctccaccaat tccgagattt cacccagttc
     3421 ggtattcata cctgtggaaa tcacaagtcc ttctcctgaa ccgcgggtaa ctgcggtacc
     3481 tttgaagagc atattggatc gctctgccag gggaacatcc tggtccagcg cctcgacctg
     3541 cttggaaacg ggcatcgatt cccccgtcaa agcggattca ttcagctgaa gcttattagc
     3601 ctccagcaaa cgcatatcgg cggttactat gtcgcccccc tccagaatga ctatgtctcc
     3661 gggcacgatc tcgcgggcat ttatattttt ggcctcacca tctctcctga ccttggtggt
     3721 aaccttactc atctcccgca ggctttgcat cgatttaacc gctttcagtt cggtgaaaaa
     3781 cccgatcaat gcgtttatta gaatcgcccc ggccaccgca aagccttgaa tcacctctcc
     3841 gaatatgaac gacagagctg aagcaaccgc cagtatcaga ataaccaggc tcttaaattg
     3901 atcggcaaaa atgctccaga tgcttctctc ctccacctcg cgaagaagat tcttgccgaa
     3961 tttgctctgg cgctcggccg cttctgatga cgagagcccc ttattccggt ccacatcaag
     4021 ttccctcaac aattcttccg cgccgcgccg ccagggctct ctgctgtctt tgttgactct
     4081 tacgtcagat tttgtatttg ccataaacat atactaatcg gtaaattcgt tgccgatcct
     4141 gtggctcttg agagtatagc tcgtctcgta tgaattaaaa aggtccagat aattttcaag
     4201 ataacggctc aggagatact tcttcttgac tgattcccgt gccttctgac ccattttatc
     4261 gcgcaatttt ttatctttta gaagtttaag aattctttcg gctgtctcat caaccgagga
     4321 aaccaggtaa ccgttctcac cgtcctggat ctgctttctg a
//
LOCUS       DSEB01000470            4233 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001931, whole genome shotgun sequence.
ACCESSION   DSEB01000470 DSEB01000000
VERSION     DSEB01000470.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001931"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="ENO07_08370"
     CDS             complement(<1..1014)
                     /locus_tag="ENO07_08370"
                     /inference="COORDINATES: protein motif:HMM:PF00258.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HDS02018.1"
                     /translation="MNYGDNMKPLEIKKNVYWVGAVDWDLRDFHGYSTEKGTSYNAYL
                     VVDKKTVLFDTVKVELQKDLLANIRQVTDPSKIDYIVVNHAEMDHSGSLPEVLKIVKP
                     EKIFCTAKCKDALIQHFHDESWPFEVIKEGDSIELGQKTIQFFDSKMMHWPESMVSYI
                     PEDNLLISNDIFGQHWATSERFDDKVDQGELYWQSAKYYANIFMPFATPAQKFLKKLK
                     ENDLKIDMIAVDHGLIWRKDIDKIIELYDKWSSGRADKKAVVVYDTMWGSTKKMARAI
                     GKGIASQDVSVQVLDLRFNHRSDVITEALDSRAIILGSPVMNNNILPGMGGFLTYMKG
                     LRPK"
     gene            complement(1121..1573)
                     /locus_tag="ENO07_08375"
     CDS             complement(1121..1573)
                     /locus_tag="ENO07_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HDS02019.1"
                     /translation="MNFNSVDEILDFAIGEEEKAADFYTELAGKVSKPNIKEMFEQFA
                     REEKGHKAKLLAAKQGKMLQKSEKKILDLKIGDHLESVELTPNIDFQDALILAMKAEK
                     NAFKLYNSLAEQADDPAIRDMLLALAQEEAKHKLRFETEYDELVLTEN"
     gene            complement(1610..2122)
                     /locus_tag="ENO07_08380"
     CDS             complement(1610..2122)
                     /locus_tag="ENO07_08380"
                     /inference="COORDINATES: protein motif:HMM:PF02915.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS02020.1"
                     /translation="MSDEQKKLTEGLKKAISAERDGYSFYMMAAGSTEDEKGKEVFET
                     LAREELDHMNFLRQQYDSILKTGRPDTSISLGPRKDLTGISPIFSDKLKSRISDAHIE
                     MSALSIGVQLELDAIRFYQEQAKETSYPDVRRFYQELADWETGHYNALWEQQQQLKED
                     YWSTGGFAPF"
     gene            complement(2168..2680)
                     /locus_tag="ENO07_08385"
     CDS             complement(2168..2680)
                     /locus_tag="ENO07_08385"
                     /inference="COORDINATES: protein motif:HMM:PF02915.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS02021.1"
                     /translation="MTLEEAIKMALEYETKVRDTYIDAARKASDDTGKRIFKVLGEEE
                     QGHINYLQSRLAEWQKTGKITTEKLDTVVPSKKAIEDGVKKLDKEMSRSDYGSEMEML
                     RKALELEIETSSFYQRMVDEMGEEGKMFEHFMKIEEGHKAIVQAEIDYLSKTGYFFDF
                     QEFSMESPIE"
     gene            complement(2793..3296)
                     /locus_tag="ENO07_08390"
     CDS             complement(2793..3296)
                     /locus_tag="ENO07_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007258826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HDS02022.1"
                     /translation="MLSDNMKKALNDQINAELYSAYIYLSMAAYFEDANFPGMATWMK
                     AQAQEEVGHAMKFYAYVFDRDSKVELKAIDGPQTEWKSPLDAFETAYNHEKHVTDLIH
                     KLVELANKENDHATRSFLNWFIDEQVEEEASTKAIVDRLKFIGDGKPGLFMLDRELAG
                     RGSGGGE"
     gene            complement(3451..3891)
                     /locus_tag="ENO07_08395"
     CDS             complement(3451..3891)
                     /locus_tag="ENO07_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS02023.1"
                     /translation="MSTKELMEEIVKNMKRWQAIEDSAVSSTGKIIEKTDNPLIRMVM
                     EIIQHDSQMHHRVQEFIARSMTEESIAITPEDLEKVWSEVENHIRIEEKTIEMAEKSL
                     EGLKGRKLVVPEYFLHYLLKDENKHNSLLQDLSVIKKNMYPYGS"
BASE COUNT          985 a         1095 c          894 g         1259 t
ORIGIN      
        1 ctttggacgc agacctttca tataggtcag gaatccgccc ataccgggca ggatgttatt
       61 attcataacc ggcgaaccca ggatgatcgc ccgagagtct aatgcctcgg tgatgacatc
      121 gctcctgtga ttgaagcgca gatcgagaac ctgcaccgaa acatcctgcg aggcgatacc
      181 tttaccgata gcgcgcgcca tctttttggt cgaaccccac atggtgtcgt aaaccaccac
      241 agcctttttg tcggccctgc ctgagctcca tttgtcgtag agttcgatta tcttatcgat
      301 atctttgcgc caaatcaggc cgtgatcgac cgcaatcata tcgatcttaa gatcattctc
      361 tttgagcttc ttcaggaatt tctgcgccgg agtggcgaaa ggcatgaaga tattggcata
      421 atactttgcc gactgccagt agagttcgcc ctggtcgacc ttatcatcaa aacgttcact
      481 ggtggcccag tgctgcccaa agatatcatt tgagatgagc agattgtctt cgggaatata
      541 actgaccatg ctctccggcc agtgcatcat cttggaatca aaaaattgaa tcgtcttctg
      601 ccccagctcg atcgaatcgc cttcttttat gacctcaaac ggccagcttt catcatggaa
      661 atgctgtatc agggcgtcct tacatttggc tgtacagaaa atcttttccg gtttgactat
      721 cttgagtacc tcagggagtg atccggagtg atccatttcg gcatgattga caacaatata
      781 atcaatcttt gatggatcgg ttacctgccg gatgttagcc aggaggtctt tctgaagttc
      841 gactttgacc gtatcgaaaa gcacggtctt tttgtccacc accaagtagg cgttgtagct
      901 tgtacctttt tcggtggagt agccatggaa atcacggaga tcccagtcaa ctgcccccac
      961 ccagtaaaca ttctttttaa tctcaagcgg tttcatattg tctccataat tcatatcttt
     1021 gcattattgc aggtcaaatc cctgtgggat ttgacgcttt gtcaaatttc acagacattt
     1081 gacctacaaa agtaggccac ccatgttgtc caattaaacc ctaattctcg gttaagacca
     1141 gctcatcgta ttcggtttcg aaacgtagct tgtgcttggc ctcctcctgg gccagggcca
     1201 ggagcatgtc ccggatggcg ggatcatcag cctgctcggc cagagaattg tacaatttga
     1261 aagcattttt ttcagctttc atggccagga tcaaggcatc ctgaaaatcg atgttgggag
     1321 taagctcgac tgactccaga tgatcgccga tcttcaggtc gagaattttc ttttctgatt
     1381 tctgcagcat tttgccctgc ttggcggcca ggagtttggc tttgtggcct ttttcctcac
     1441 gggcgaactg ctcgaacatc tccttgatat tcggcttgct cacttttccg gcaagctcgg
     1501 tataaaaatc ggcggccttt tcctcctcgc cgattgcaaa atccaatatt tcatccacag
     1561 aattgaagtt catcttaacc tcggtatttt aagttgattg ccacccggtt taaaacggcg
     1621 cgaaaccgcc cgtggaccag taatcctctt tcaattgctg ctgctgttcc cagagagcgt
     1681 tgtaatgccc ggtttcccag tcagccagtt cctgatagaa tctcctgaca tcgggataac
     1741 tggtctcctt ggcctgctcc tgatagaacc tgatcgcatc cagttccagc tgtaccccga
     1801 tggaaagcgc gctcatctca atatgcgcat cgctgatccg cgatttaagt ttatccgaga
     1861 aaatcgggga gatgccggtg aggtctttgc gcggaccgag gctgatactg gtatccggtc
     1921 tgccggtctt cagaattgaa tcgtactgct ggcgcaggaa gttcatgtga tccagctctt
     1981 cgcgcgccag agtctcgaac acctcttttc ctttttcatc ctcggtgctt ccggcggcca
     2041 tcatatagaa gctgtagccg tcacgttcgg ccgaaattgc cttcttcagg ccctctgtaa
     2101 gtttcttttg ttcatcagac ataatgaaat cccataattt acgtattcaa aagatcctct
     2161 gatcgtccta ttcaatcggc gattccatcg agaattcctg gaagtcgaaa aagtagccgg
     2221 ttttgctgag atagtcgatc tccgcctgca ctatggcctt gtggccttcc tcgattttca
     2281 tgaaatgctc gaacatcttg ccttcctcgc ccatctcgtc aaccatgcgt tggtagaagc
     2341 tggaggtctc gatttcaagt tccagggcct tgcgcagcat ctccatctct gagccgtaat
     2401 cagacctgga catctcctta tcaagtttct tcacaccatc ctcaatcgcc ttctttgagg
     2461 gcacgacggt atccagtttt tccgtggtga ttttgccggt cttctgccat tcagcgagcc
     2521 tgctctgaag gtaattgata tgtccctgtt cctcctcgcc aagaaccttg aatatccgtt
     2581 tgccggtatc atccgaggcc ttcctggcgg catcaatata ggtgtcgcgc actttggtct
     2641 catattcgag cgccattttg atagcttctt caagagtcat tttatcctcc ggattcagtc
     2701 cgtttctgta atcgatactg gaaaagagag ggcatccatc acccgggtga tgaatgccct
     2761 tcacaatata ttattcaaga tcaaacatcg gcttattcac cgccgcccga tcctcttcct
     2821 gccagctcgc gatcgagcat gaacaggccg ggcttgccgt cgccgataaa cttcagcctg
     2881 tcgactatcg ccttggtcga ggcctcttcc tcaacctgct catcgataaa ccagttcaaa
     2941 aatgacctgg tggcatgatc gttctcttta ttggccagct caaccagttt gtggatcagg
     3001 tcggtgacat gcttctcatg attgtaagcg gtctcaaagg catcgagagg ggatttccat
     3061 tcagtctggg gaccgtcgat tgccttcagc tcaaccttcg aatcacggtc gaagacataa
     3121 gcatagaatt tcattgcatg tccaacctct tcctgggctt gggccttcat ccaggttgcc
     3181 atgcccggga agttagcatc ttcaaaatat gcagccattg aaagataaat ataagctgaa
     3241 tagagttcag cattaatttg atcattcaag gcctttttca tattgtcgct taacatttct
     3301 attttccttt ccaaatcacc ctttgttcag tcattaataa ttatctatga caaaacaaca
     3361 ctgaattact aatgtttccg aaatccagca gggatttccc tttttttgag ggatttcaca
     3421 tcccatttat atatagaaaa tatccgacga ttatgaaccg tacggataca tatttttctt
     3481 tattaccgaa agatcctgta gaagcgagtt gtgcttgttt tcatccttca gaagataatg
     3541 cagaaaatat tcgggcacaa caagtttgcg gcccttcagg ccttccagag atttctcggc
     3601 catctcgatc gtcttttcct cgatacggat atggttctcg acctctgacc agaccttctc
     3661 cagatcctcg ggagtaatcg caatagattc ctcggtcatc gagcgcgcga tgaactcttg
     3721 cactctatga tgcatctggg agtcatgctg gatgatttcc attaccatgc gaataagcgg
     3781 attatcggtc ttctcgatga ttttgccggt tgatgacaca gctgaatcct cgattgcctg
     3841 ccagcgcttc atattcttga caatctcttc catcaattcc ttggtggaca tcttttcctc
     3901 ctcaataatt ctgttataat ttactattta ctctttcaat cccttttatt cttccgagag
     3961 ggtctgtttt ccaccccgac agagagtcct taaaaggctg tgaaatatat cacataatcc
     4021 ttgaaaaatc aagggcggaa aaacctgccc ttatgatttc cgattctaat tccagcattc
     4081 cctccggacc aatttaattt tccatccaag atagaacaag ccattttcaa aatggttcgg
     4141 gggccggtcc cgaattttgc cgattctcta aaatctccgg taaaagccgg atttctttat
     4201 tggcccggtc atcgcattct tatatattaa tcc
//
LOCUS       DSEB01000471            6967 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1000646, whole genome shotgun sequence.
ACCESSION   DSEB01000471 DSEB01000000
VERSION     DSEB01000471.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6967)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6967)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6967
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1000646"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..446)
                     /locus_tag="ENO07_08400"
     CDS             complement(<1..446)
                     /locus_tag="ENO07_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS02024.1"
                     /translation="MNNRIAAIGTINRDTIHLPSGRKYESYGGLLYSIIALAQLTEDD
                     YEILPVVNLGSDCNEAVMGILKKYDKIKTSAIRIVPAKNNHCNLYYLDRETKTEVLKG
                     GVPTLKFEDLAPALESELTLVNFISGNDVSLDALEEFRRNYSGKIY"
     gene            complement(439..1461)
                     /gene="waaF"
                     /locus_tag="ENO07_08405"
     CDS             complement(439..1461)
                     /gene="waaF"
                     /locus_tag="ENO07_08405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HDS02025.1"
                     /translation="MTNSKMKKVIIRFPNWLGDCIMAAPVLTALRNAHPEWEVTLLVR
                     GYLSQLFRSDPRVDHVIELKDKSDSFNPTGFFELADELGQAGYDTGIILPDSFSSALI
                     FFMAKIPTRIGYKAQMRRFMLTDALDQPKKIIHRSQKYLRLLKSVDVIPHVDVEPEIF
                     PDDESRQRAELLARGLDRYIVITPQTNAPSRRWGYDKYSKLIARVVKALKLQVVLLGA
                     ENEYDVVEKVGSDSGVQFLNLAGRASLPVSYGIMKEALCFVGNDSGAAHLAAAAGTYT
                     ISISGADNPEETRPLAARGKIIRSDLPCISCVKNICPRKDVPMECMHVISVEEVFEAV
                     RETVDE"
     gene            complement(1461..2345)
                     /locus_tag="ENO07_08410"
     CDS             complement(1461..2345)
                     /locus_tag="ENO07_08410"
                     /inference="COORDINATES: protein motif:HMM:PF03279.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS02026.1"
                     /translation="MKSLRRGIEFFLLKLLAAFIASLPERTSLHCGRILGTSIYYLLG
                     RRKIVAYKNMSLCAIGDDRKLRSKILHRCLENFGMTAVEFLRQNTYSVHDYRRKVRIK
                     DFSPFDRAFEKQRGVFLFSGHYDNWELLIQGVAAYGYETTMLVRQQHNLKVDDLVNSL
                     RKNGRIDVIIAESSPRQILRALQNGRGVATLLDIWGGKDGRMADFFGHQVSTPAGVLE
                     IAVRQRTPILTGFLERFKDGSHRLENVEVIFPGDDDRFPGVDSIVEYYHRRLENAIRR
                     KPIFWLWTHKRFKNIVDY"
     gene            complement(2320..3249)
                     /locus_tag="ENO07_08415"
     CDS             complement(2320..3249)
                     /locus_tag="ENO07_08415"
                     /inference="COORDINATES: protein motif:HMM:PF13485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDS02027.1"
                     /translation="MFSNGFRNIVNAIVLVLFLSSVAFAYEQVNIFKIQAPDKDLFER
                     TRISLLHAKTDLERLLGEEVTDTIRVFIAASRFDFDTAAFGGIPDWGVGVAIPSRNYI
                     SVLSPTGKNMNMPFEMVVRHELAHVAVSKRVDGKRIPRFMDEGFAMMFAHQWGFGDDI
                     TISTAQLTGSLYELWEIDAVNMLNSAQARIAYAQSYLAVKYIFDVFGKETFQSLLDGF
                     RMGQDRNTVFRNVLGTDFAGFDKMFADHLKSNYHWIMIITNPIVLWIGVVLLFLLGFI
                     LIRKRNKDIYKKWEEEEKLQSTDFDYEESSPWD"
     gene            complement(3268..3825)
                     /gene="cysE"
                     /locus_tag="ENO07_08420"
     CDS             complement(3268..3825)
                     /gene="cysE"
                     /locus_tag="ENO07_08420"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017305987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDS02028.1"
                     /translation="MRTLIEDIKAIYRNDPAARNIEFLFYAGLWAITLHRITHFLWKA
                     HIPLIPRILSQLTRFFTGVEIHPGATIGRGFFIDHGMGVVIGETTEIGNNCVLFHNVT
                     LGGTGKHKKKRHPTLGSGVFVGTGATLLGPLNVGNNVRIGANTFIIMHDVPDNCTVVG
                     SPGKIVIMDGKRVDIPLLPTKEPND"
     gene            complement(3842..6250)
                     /locus_tag="ENO07_08425"
     CDS             complement(3842..6250)
                     /locus_tag="ENO07_08425"
                     /inference="COORDINATES: protein
                     motif:HMM:PF05170.12,HMM:PF13502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDS02029.1"
                     /translation="MSKGLKILIWAVSIILVLALLSIAAIKIFFPAEKVQALIVENLQ
                     KSLNRPVTVKNASVSIWGGVGLKLDSLVVQNLPGFSGEEFFVLDKLDVKLKLWPLITG
                     NFEFTKIILDGLAVNLERARSGALNFEDLGQHKEEEVEPAGGATSIPFLFHKLSVENS
                     RLNFYDDSSGYTFSLDGMSLNSELKKNGFEEAYYAQGQFGIDNIIIRNPEKKIEFPHL
                     ALDADLQMKLFTKADMLEIEKIDIRLADLQGQLTGKLTSLMNNPACNLDFRTKRFSVE
                     QLLSLVPPDLSPAVNNLEGSGDMFATASVRGPLNPPGELDVSGRVSFENVVFSSPRVD
                     GDFKIQSGEINLRQKAANLLFENCTFAGEPFSLKVYAKNIIEPVINADVSMSINLRTF
                     RDYSDGINELSGRVYGQLNITSDLQNPVSTVIDGGIVLEDLILSHVDLEQKVEEVDME
                     LEFKQRDINLENLQAEMGNSRFALDGTMKDIIPFMADPESDKYEPAITFELNADYLNL
                     DELQEMMPWDTAKVRPGPETALEAEGLPQFTAQGSLRIERGTYSKVEFENFTGSLDYR
                     DNILHLASMRADMYDGQAEGEAIVDYEDFYNPEFQVDFNADNIEINSFLSRLTPLENV
                     LFGKARMSSSFTGMVGEPSEVLDSLTANGKVNMSEGRIENLPVMEKLADQLGLKTFEE
                     KQIRNLYNTFRVEDGRVLFDNFNMSIGNTGWNINGSAGFDGSLDYVVGIEMDKSSLGS
                     DALGGLQSILGGGDGRITIPVKLTGSYNDPKFSVDRSKLQESADKKLEEEGKKLLDNL
                     FKDN"
     gene            complement(6303..6938)
                     /locus_tag="ENO07_08430"
     CDS             complement(6303..6938)
                     /locus_tag="ENO07_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010532395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDS02030.1"
                     /translation="MSDGTNNLIIDPFLSGNPKAKTAPEDVEANFVLVTHGHGDHWGD
                     TEKIAKNNDALVIGPNEVAAYAARKGLNAHNMHIGGAHDFPFGRVKLTIAHHGFSDGE
                     SDFDIGGSPCGFIITMEGKKIYHAGDTGLFMDMQLIGEEGIDLAMLPIGDNFTMGIDD
                     AARAVDFIKPKKVIPMHYNTFELIAADPEEFRKKVKDSEVIILGVGDETQL"
BASE COUNT         1760 a         1804 c         1563 g         1840 t
ORIGIN      
        1 atatatattt taccgctgta gttcctgcgg aattcctcga gcgcatcgag gctgacatca
       61 ttaccggaaa taaaattgac caatgtcaat tcgctttcca atgcgggcgc caaatcctca
      121 aatttaagag tcgggacccc gcctttcaat acttcggtct tagtctcccg gtcaaggtag
      181 tatagattac agtgattatt ttttgcgggg acgattctga tcgcagatgt tttgatttta
      241 tcatatttct tcaggatgcc cattacagct tcattgcaat cacttcccag attcactacc
      301 gggagaatct cataatcatc ttcggtgagc tgcgcaagcg ctataataga gtacagcaga
      361 ccgccgtagc tttcatattt gcggccggag ggaagatgga tagtatcacg atttattgtc
      421 ccgatcgcgg caattctatt attcatcaac cgtttccctc accgcctcaa atacctcctc
      481 cacacttatt acgtgcatac attccatagg aacatctttt ctggggcaga tattcttgac
      541 acacgagata cacggcagat cactgcgaat gatctttccc cgggctgcca gtgggcgagt
      601 ctcctccggg ttatccgcac cagatatgga gatggtgtaa gttcctgcgg cggccgccag
      661 atgcgcagcg ccgctgtcgt tgcccacaaa gcaaagagcc tccttcataa ttccatatga
      721 caccggtagc gaggcacgac cggcgagatt taaaaattgg actccggaat ctgatccgac
      781 cttttccaca acatcgtatt cgttctctgc gcccagaaga acgacctgaa gtttcaaagc
      841 cttaacaact cttgcaatca acttggaata cttatcatat ccccagcgcc ttgagggagc
      901 attagtctgc ggagtgatca cgatatatct atccagacct cttgccagta gttcggcacg
      961 ctgcctgctc tcatcatccg gaaatatttc cggttcaacg tcaacatgag gtataacatc
     1021 gacactcttc agcaaacgca ggtatttttg ggagcgatga attatcttct ttggttgatc
     1081 gagagcatct gtcagcatga acctgcgcat ctgggcctta tatccgatcc ttgtgggaat
     1141 ttttgccata aagaatataa gggcggatga aaatgagtcg ggcagtatta ttccggtatc
     1201 atagccggcc tggccaagct cgtcagcaag ttcaaagaat ccggtcggat taaaggaatc
     1261 acttttgtct ttgagctcga ttacatgatc cacgcgcgga tcgcttctaa ataactgcga
     1321 cagatatccc ctgacgagaa gagtaacctc ccattcggga tgagcattgc gcagagcagt
     1381 aagaaccggc gcggccatta tgcaatcgcc cagccagttg ggaaatcgaa taatgacttt
     1441 tttcattttg ctgtttgtca tcagtaatca actatatttt tgaagcgttt atgagtccag
     1501 agccagaaaa taggttttct cctgatagcg ttctccagac gccgatgata gtattccact
     1561 atggaatcaa cgcccggaaa tctgtcatcg tccccgggga aaatgacctc gacattttcg
     1621 agccgatgac tgccgtcttt aaacctctcg agaaaaccgg tcaggatcgg cgttctctgc
     1681 cgcacggcga tttccagtac accggccggg gtggagacct gatgtccgaa gaaatcagcc
     1741 atccttccat ccttaccacc ccagatatcc agcagggtag cgactccccg tccattttgc
     1801 agggcacgca atatttgccg gggactggac tcggcgatta taacatctat tctcccattt
     1861 ttgcggaggg agtttaccag atcgtcaacc ttgagattat gctgttgacg aacaagcatg
     1921 gtagtctcat atccgtatgc tgcgacaccc tgtatcagca attcccagtt gtcataatgc
     1981 cctgaaaaca agaaaactcc gcgttgcttt tcaaatgccc tgtcgaatgg ggagaaatcc
     2041 ttgatacgca cttttctgcg gtagtcatga actgaatatg tattctggcg cagaaattcg
     2101 accgcggtca tcccgaaatt ctcaaggcac ctgtgcagga ttttactgcg cagctttcga
     2161 tcatctccaa ttgcgcacaa gctcatattc ttgtatgcga ctattttgcg ccgtcccagc
     2221 agataatata tcgatgtgcc tagaatacgg ccgcaatgaa gtgatgttct ttcgggcagt
     2281 gaagcgatga aggcggcaag cagcttcagc agaaaaaact caatcccacg gcgaagactc
     2341 ttcatagtcg aaatccgtac tctgcagttt ctcctcctct tcccatttct tgtagatatc
     2401 cttatttctc ttgcgtatca ggataaagcc aagcagaaac agaagtacga cacctatcca
     2461 gaggactatg ggatttgtta tgatcataat ccaatgataa ttgcttttta agtgatccgc
     2521 aaacatttta tcgaatccgg caaaatccgt cccgagcaca ttacggaaaa ccgtatttcg
     2581 atcctgcccc atgcgaaagc cgtccagaag gctttgaaag gtctctttac caaagacatc
     2641 gaatatatat ttcacggcca gatatgactg cgcataagct atgcgcgcct gggctgaatt
     2701 taacatattc accgcgtcaa tctcccacag ctcataaagc gatccggtga gctgggcggt
     2761 ggaaatggtg atgtcatcgc cgaatcccca ttgatgcgcg aacatcatgg caaacccctc
     2821 gtccataaaa cgcggtatgc gcttgccgtc aacacgtttg ctgacggcca catgggccag
     2881 ttcgtgcctg accaccatct caaagggcat attcatgttt tttccggtcg gcgaaaggac
     2941 cgagatataa ttccgggagg gtatggctac gcctacgccc cagtcgggaa ttccgccgaa
     3001 tgccgcggta tcgaaatcga atctgctcgc cgcgatgaaa accctgatgg tatctgtaac
     3061 ttcctccccc agcaggcgtt ccagatcggt cttcgcatga aggagggaaa ttctggtgcg
     3121 ttcaaacagg tctttatcgg gcgcctgtat tttaaagata ttgacctgct cataggcgaa
     3181 tgcgaccgat gagaggaaca gcaccagtac aatggcgtta actatatttc gaaaaccatt
     3241 agagaacatt attactataa agcaaaatca atcatttggt tccttggtgg gcagaagcgg
     3301 gatatcgaca cgtttgccat ccatgatgac gatttttccc ggtgacccga ccacagtgca
     3361 attatccggg acatcatgca ttataataaa cgtgttcgcg ccaatccgga cgttgttccc
     3421 aacattgagc ggccccaaaa gtgttgcccc ggtgcccaca aaaacaccgc ttccaagagt
     3481 gggatgtcgt ttctttttat gtttaccggt accgcccagg gtaacattat ggaaaaggac
     3541 gcagttgttg ccaatttcag tggtctctcc tattaccaca cccataccat gatcgataaa
     3601 gaaacctctg ccgatggtgg ctccgggatg tatttccact ccggtgaaga atctcgtcag
     3661 ctgcgacagg atacgtggta tgagggggat gtgggctttc cacagaaaat gggttatgcg
     3721 atgaagggta atcgcccaca atccggcata aaacaggaat tcgatgtttc gggcagccgg
     3781 atcattgcga tagatcgcct ttatatcctc aattaatgtt ctcactctta aatatcaggc
     3841 gctaattatc cttaaacaga ttatcgagga gtttcttgcc ctcctcctca agttttttat
     3901 cggcagattc ctgcagcttt gaacgatcga ccgaaaattt gggatcattg tagctcccgg
     3961 tcagcttgac cggaatggtg atcctgccgt ccccaccgcc cagaatcgac tgcaatccgc
     4021 caagcgcgtc cgaaccgagg ctgctcttat ccatctcaat accgacaaca tagtcgaggc
     4081 tgccgtcgaa tcccgccgaa ccattaatat tccagccagt gtttccaata ctcatgttga
     4141 aattatcaaa cagcactctg ccgtcttcga cccgaaatgt gttgtataaa tttcgaatct
     4201 gcttctcttc aaatgtcttg agtccaagct gatctgccag tttttccata accggaagat
     4261 tctctatgcg tccctcactc atattgactt tgccgttggc ggtcaatgag tcgagcacct
     4321 cggagggctc gccaaccata cccgtgaatg atgagctcat gcgggccttg ccgaagagca
     4381 cattctccag cggagtcagc cgcgaaagaa agctgttgat ctcaatatta tcggcgttaa
     4441 aatcaacctg aaactcggga ttgtagaaat cctcgtaatc tactatcgcc tctccctcag
     4501 cctggccgtc atacatatcg gcgcgcatac tggccagatg caaaatgttg tcgcggtaat
     4561 ccaggctgcc ggtgaaattc tcgaactcta ccttagaata ggttcccctc tcgatccgca
     4621 ggctgccctg tgcggtaaat tgagggagcc cctcggcctc cagcgcagtt tcgggcccgg
     4681 gcctcacttt tgctgtatcc cacggcatca tctcctgcag ttcatccagg ttcagataat
     4741 cggcattaag ctcgaaagtg atggccggtt catatttatc cgattcggga tcagccataa
     4801 acggtattat atctttcata gtgccgtcaa gagcgaaccg ggagtttccc atttctgcct
     4861 gcagattctc caaattaata tcgcgttgtt taaactccag ctccatgtcg acctcttcca
     4921 ctttctgttc gagatcaaca tgtgaaagga ttaaatcctc gagaactatc ccgccatcta
     4981 ttacggtaga aaccggattt tgcaggtcgc tggtaatatt caactggccg taaacccgtc
     5041 cggaaagctc gttaatgccg tctgaataat ctctgaaagt gcgcagattg atgctcatgg
     5101 agacatcggc gtttatcaca ggttcgataa tgttttttgc atagaccttt aatgaaaatg
     5161 gctctccggc aaaagtgcaa ttctcgaaga ggagattggc cgccttctgc ctgaggttta
     5221 tttcgccgga ctggattttg aaatcaccat ccacccgcgg actggaaaaa acaacatttt
     5281 caaatgaaac tcggccggaa acatcaagtt cgcccggcgg attgagtggt ccccgtactg
     5341 aggcggtggc aaacatatct ccgctgccct ctaaattatt cacagcaggg ctcaaatcgg
     5401 gcggcaccag gctcaataac tgctcgaccg aaaagcgctt ggttctgaaa tccaggttgc
     5461 aggccggatt gttcatcaga gatgtgagct taccggttag ctgcccctgc aggtcagcca
     5521 gcctgatatc gattttttcg atctcgagca tatcggcttt ggtaaaaagt ttcatctgca
     5581 gatccgcatc cagagcaaga tgcgggaact cgatcttttt ctcgggattt ctgatgatga
     5641 tattgtctat tccaaattgg ccctgagcat aataggcctc ttcaaatcca tttttcttaa
     5701 gctccgagtt caagctcata ccgtcgaggc tgaaggtata tcctgaggag tcgtcataga
     5761 aattcagccg cgaattttct acactcagct tgtggaacag gaatggaata gaggttgcac
     5821 cccctgcagg ttccacttcc tcctctttat gctgacccag atcctcgaaa ttcaatgctc
     5881 cgctccgggc tcgttccaga ttgactgcca ggccgtccag gattatcttg gtaaattcga
     5941 aattcccggt gataagcggc cacagtttca gttttacgtc aagtttatcg aggacaaaga
     6001 actcctcgcc ggaaaaaccc ggcaggttct gcaccaccag actgtccagc ttcagtccta
     6061 caccgcccca tatagacact gaggcgtttt taaccgtaac agggcggttc aaggattttt
     6121 gcaggttttc gacaatcagc gcctgcactt tttcggcagg aaagaatatt ttgattgcag
     6181 caatcgaaag cagagccagt accagaatta tcgataccgc ccagattagt atttttaacc
     6241 ccttcgacac aagctatccc tctttcaatt aaaaggtttt ctttttatac agagctccaa
     6301 tactaaagtt gcgtctcgtc accaacaccg agaatgatga cctcggagtc tttgaccttc
     6361 tttctgaatt cctccggatc ggccgcgatt agctcgaacg tgttgtaatg catggggata
     6421 actttcttgg gcttgatgaa atctactgcc cgcgcggcat catctatacc catagtaaaa
     6481 ttatcgccaa taggcagcat ggcaaggtct ataccctctt ctccaatcaa ctgcatatcc
     6541 ataaaaagtc ctgtatcccc ggcatgatag attttcttac cttccattgt gataataaag
     6601 ccgcatggac ttccgccgat atcaaaatcc gactcgccgt ccgaaaagcc gtgatgagcg
     6661 attgtcagct tcacccttcc gaagggaaaa tcgtgtgcgc cgccgatatg catgttatgg
     6721 gcgttcagcc ccttacgcgc ggcataagct gccacctcgt tgggcccgat aacaagggca
     6781 tcattatttt ttgcgatctt ctctgtgtca ccccagtgat cgccatgacc atgggtcacc
     6841 aggacaaaat tggcttcaac atcctccgga gcggttttgg ccttgggatt gccgctcagg
     6901 aagggatcga taatcagatt attggttccg tcggagatcg taaagcagga atgactgaga
     6961 tatgtaa
//
LOCUS       DSEB01000472            4686 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1001520, whole genome shotgun sequence.
ACCESSION   DSEB01000472 DSEB01000000
VERSION     DSEB01000472.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1001520"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            85..612
                     /locus_tag="ENO07_08435"
     CDS             85..612
                     /locus_tag="ENO07_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS02031.1"
                     /translation="MRYKNLFLLVLGAMLLFGGALLIAGDETAKKAGKSWFDMENCAF
                     CKNLIAEPGLLEHSDWENHKIDNGAIMVTTVEDEYLPAMRKVNANMMAVAEKLQKGEQ
                     LYLCGMCSAYGELWMSGAKIQTVETNHGYVEIFTSDDPEMVAKIHAFADKNNEEMARL
                     MEMEMEHGHEAEEAD"
     gene            complement(673..1077)
                     /locus_tag="ENO07_08440"
     CDS             complement(673..1077)
                     /locus_tag="ENO07_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019419553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDS02032.1"
                     /translation="MIYTSSTEDINPENLQGFFVGWPNPPSTQTFLKILKQSHEVVLA
                     VDEATDNVVGFINAISDGILSAYIPLLEVLPEYKGRGIGSELVRRMLRKLDGLYMIDL
                     VCDQELQAFYTKIGLKPGFAMMIRNFKKQSGK"
     gene            complement(1533..4040)
                     /gene="gyrA"
                     /locus_tag="ENO07_08445"
     CDS             complement(1533..4040)
                     /gene="gyrA"
                     /locus_tag="ENO07_08445"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDS02033.1"
                     /translation="MERENILPLSLVEEMQTSYLDYSMSVITARALPNVADGLKPSNR
                     RILIAMNDLNLTPGRGFRKCAKIAGDTSGNYHPHGEGVVYPTLVRMAQDFNMRYTLVE
                     GQGNFGSIDGDPAAAMRYTEARLTPLAVEMLADLEKNTVDYVPNYDETREEPVVLPSK
                     FPNLICNGSSGIAVGMATNIPPHNLNEIVDALTAIIDNPEMENDELLEYVTAPDFPTG
                     GIIYGRKGIRDAYLTGQGRVIVRAKANTEKQKSGKESIIISEIPFQVNKSNLLEKIAY
                     LVSNKVIEGITDLRDESDRDGMRIVIELKRDAQAPVILNQLFKHTQMQTTFGVNMLAL
                     VDGRPVTLTLRQILDEFIKHRHTVVTRRTQYELEQAEHRAHILEGFKVALDHIDAIIT
                     LIRNSNTPAVAKERLMKEYQLSDIQAQAILEMRLQRLTGMEREKIEGEYLDVIKKIEK
                     LQGILKSKSKRMKIIKDELAELKKKYGDERRTLVTDEEEEISLEDLIAEEDMVITITH
                     SGYIKRISISSYRRQKRGGRGVIGMETKEEDLIEHLFVASTHDYILFFTNKGKCYWIK
                     VHELPVGGRLSKGRPIVNMLSLEKDETICAFLPVREFVEGRYIVMATRQGQVKKTDLT
                     AFSNPRKDGIIAMSLPDKSKDELIEVGLTDGTNEVILATRNGQAIRFSEENVRPMGRT
                     AYGVKGINLGKGDYVISMVVVNRMTTLLSVTENGFGKRSEIADYRITKRGGKGVINIK
                     ATERNGKVVAVREVVDSDEMIMITNKGIVIRSHVGAIRTIGRATQGVKLINLDKGDKV
                     IDVARVVSDKETNGLDKNEPINGESDFSDEEEDTANE"
     gene            complement(4106..>4686)
                     /locus_tag="ENO07_08450"
     CDS             complement(4106..>4686)
                     /locus_tag="ENO07_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545530.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDS02034.1"
                     /translation="LPLRGKIINVEKARLDRILSNEEIKTIITALGAGIGRDNFNMEK
                     LRYYKIIIMTDADIDGAHIRTLILTFFFRHMRELVESGHIYIANPPLYRISHGKTEQY
                     VYSDDERDRYLEKIGRDKKIDIQRYKGLGEMNPDQLWKTTMDPDTRTLFKVTLEDAAA
                     ADRIFTILMGDVVGPRKEFIQNNAQYVRNLDV"
BASE COUNT         1042 a         1174 c         1181 g         1289 t
ORIGIN      
        1 cctgaaagag ttatcattta gagcgaataa aaaatatgtg aagtattcat attttcagta
       61 aacttttttg caggaggcag gagtatgcgt tacaaaaatc tatttttgct ggttctgggg
      121 gcgatgcttt tattcggcgg cgctcttttg attgccggtg atgagacggc caagaaagcc
      181 gggaagtcgt ggttcgatat ggaaaattgt gcattttgca agaaccttat cgccgagccg
      241 ggacttctgg agcattccga ctgggaaaac cacaaaatcg acaatggtgc gataatggtt
      301 accacggttg aggatgagta tcttcccgca atgaggaagg tgaatgccaa catgatggct
      361 gtggccgaaa agcttcagaa gggcgagcag ctctatcttt gcggcatgtg ttccgcttat
      421 ggcgagctct ggatgtccgg cgccaaaatc cagactgtgg agaccaatca tggctatgtg
      481 gagatattca cttccgatga tccggagatg gtggccaaaa ttcatgcctt tgctgacaag
      541 aacaacgagg agatggcccg tttgatggag atggaaatgg agcatggcca tgaggccgag
      601 gaagctgatt agctgaattg tgtttgtggc cgccaagaag cctttcctgg cggctgcacc
      661 gctcggactt attcatttcc ccgactgctt tttgaaatta cgaatcatca tggcgaaacc
      721 gggcttcagg cctatttttg tataaaatgc ctggagttcc tgatcacaga caaggtcaat
      781 catatagaga ccgtcgagtt ttctcagcat gcgcctgacc agttcacttc cgatccctct
      841 tcctttatat tccggtaaga cctcaagtag aggaatgtag gcgcttaaga ttccgtcgct
      901 gatggcattt ataaagccta caacattgtc tgtggcttca tcgacagcca gtacaacctc
      961 gtgactctgt ttcaatatct tcagaaatgt ctgtgttgag ggcggattgg gccatcccac
     1021 gaagaagccc tgcagattct cgggatttat atcttcggtg gaggaagtat atattatcat
     1081 ggttgcccta ccggcttatt tcttatcaaa ggaggccgcg gccgtacatg ttctccctgt
     1141 atcggggtga atatatatga cattgcaata agtttggcaa gcggatttat gtcattctct
     1201 tttgtagccc acctgaaaag ggcttgttga taaaccctga atgcatcgat attcgtcatt
     1261 gcgaggagcg aagcgatttt gaatccggct cctcacggac gtggcaatct ccgcgaccgt
     1321 tcttgaggaa accgcgaaga ttcccacggt cgtcctcgcc tctggcggga ttccctcgga
     1381 atgacttgtg tcggttctta tttcaatatc tttaactttt tcaacaagcc cgaaaaggcg
     1441 gggcgaagcc agcgattcac ctgctatgta tattcttaaa atataacata gcaggagaaa
     1501 aaataaaccc acatttcatg tgggatacac gcctattcgt tggcggtgtc ttcttcctcg
     1561 tcggagaaat ctgattcgcc gttgatcggt tcgtttttgt cgaggccatt ggtttccttg
     1621 tcggaaacga cccgtgcgac atcgatcacc ttgtcgccct tgtccagatt gatcagcttc
     1681 acgccctggg tggcgcgtcc gatagtgcgg atagcgccga catgagaacg tatcacgatg
     1741 cccttgttgg tgatcatgat catttcatcg ctgtcgacaa cctctcgcac cgccacaacc
     1801 ttgccattac gttcggtggc cttgatattt atcacacctt tgcctccgcg cttggtgatg
     1861 cgataatccg cgatttcaga tcgtttaccg aagccgtttt cagtgacaga cagaagcgtg
     1921 gtcatgcggt taaccaccac catgctgatg acataatccc ccttgccgag attgatgcct
     1981 tttacaccgt aagcggtacg tcccatcgga cggacattct cctcggagaa tcttatcgcc
     2041 tggccgttac gtgtggccag tataacctcg ttggtgccgt cggtcagacc gacctcgatc
     2101 aattcatctt tggatttatc cgggaggctc atggcgatta tgccgtcctt gcgcggattg
     2161 gagaaggctg tcaggtcggt tttcttgacc tgcccctgac gcgtggccat aacaatgtat
     2221 ctgccttcca caaattcgcg caccggcagg aaggcgcaga tggtctcatc cttctcaagg
     2281 ctgagcatgt tgacaatcgg ccgtcccttg gagagccgtc ccccgaccgg cagctcgtgc
     2341 accttgatcc agtagcactt acccttgttg gtgaagaaga gtatgtaatc gtgagtggag
     2401 gccacgaaga gatgttcgat caggtcctct tccttggttt ccatcccgat caccccgcgt
     2461 ccgccgcgtt tctggcggcg gtatgaagag atcgatatgc gcttgatata accgctgtga
     2521 gttatcgtaa ttaccatgtc ctcttctgca ataaggtctt cgagggaaat ctcttcttcc
     2581 tcatcggtga ccagagtgcg cctctcatcc ccatattttt tcttaagctc tgccagttca
     2641 tccttgataa ttttcatgcg ctttgatttg gatttcagaa ttccctgcag cttctcgatc
     2701 ttcttgatga catccagata ctcgccttca atcttctcgc gctccatgcc tgtcaaccgc
     2761 tgcaggcgca tctccagaat tgcctgggcc tgtatatctg agagctggta ttctttcatc
     2821 agcctctcct tggccacggc cggagtattg gaatttctga tcagggtgat gatggcatcg
     2881 atatgatcca gcgcaacctt gaacccttct agtatatggg cgcggtgctc ggcctgctcc
     2941 agttcgtact gagttctgcg cgtgacaact gtatgcctgt gcttgatgaa ttcatccaga
     3001 atctgccgca gggtcagagt caccggccgg ccgtcgacca gggccagcat attgacgccg
     3061 aaggtggtct gcatctgggt atgcttgaat aattgattca gaataaccgg cgcctgcgcg
     3121 tcacgcttga gttcgatcac aatacgcatg ccgtcgcggt cggactcatc gcgcaagtcg
     3181 gtgatcccct caataacttt attggaaacg agataggcaa ttttttcgag caggttggat
     3241 ttgttgacct ggaacggtat ttccgagata ataatcgatt ccttgccgct tttctgcttt
     3301 tcagtattgg ctttggcgcg gacgatcact cgtccctgtc cggtcagata ggcgtcacgt
     3361 atccccttgc gtccatagat aattccgcct gtcggaaaat cgggcgcggt tacatattcc
     3421 agaagctcat cattctccat ctcgggatta tctataatcg cagtcagggc atccacaatc
     3481 tcattaagat tgtgaggggg tatattggtg gccataccga ccgcaatacc cgaggagccg
     3541 ttgcagatca agttgggaaa tttggacggc aatacaaccg gttcctcacg ggtttcatca
     3601 tagttgggaa cataatcgac agtatttttc tcgaggtcgg ccagcatttc gaccgccagc
     3661 ggggtcagcc gtgcctctgt gtaacgcatg gccgcggcgg ggtcgccgtc aatcgatccg
     3721 aagttgccct ggccctcgac cagggtatag cgcatgttga agtcctgcgc catcctgacc
     3781 agtgtgggat agaccacacc ctcaccgtga ggatggtagt tcccggaggt atcgccggcg
     3841 atcttggcgc atttcctgaa tccacgtcca ggtgtcagat taagatcgtt catggcgatc
     3901 aggattcttc tgttggatgg tttcaatccg tcagcgacat tgggaagcgc acgcgcggtg
     3961 atgaccgaca tcgaatagtc aagatagctg gtctgcatct cctcgaccag cgacagcggc
     4021 aatatattct ctcgttccat taccatgatt tatttcccct gcttactcct gtttaccctt
     4081 catacattag atttgattag actttttaaa catcgaggtt gcgcacatac tgggcgttgt
     4141 tctgaatgaa ttccttgcgc ggcccgacca catcacccat caggattgtg aaaatcctgt
     4201 cggctgcggc ggcatcctcg agagttacct taaacagcgt tctggtgtcc ggatccatag
     4261 tagttttcca gagctgatcc ggattcattt caccgagacc cttgtagcgc tgaatatcga
     4321 tttttttgtc gcgccctatt ttttccagat agcggtcgcg ctcatcatcg gaatagacat
     4381 actgctcggt tttgccgtgg ctgattctat acaatggcgg attagcgata tagatatgac
     4441 cggactcgac cagttcgcgc atatgccgga agaagaaggt caggatcagc gtgcgaatat
     4501 gagcgccgtc aatgtcggcg tcggtcatga tgatgatttt ataataacgc agcttttcca
     4561 tattgaaatt atcccgcccg atgccggcgc caagggcggt gataatggtc ttaatttcct
     4621 cattggaaag aattcggtcg agacgggcct tttcgacatt gatgatcttg ccgcgtaatg
     4681 gaagaa
//
LOCUS       DSEB01000473            3686 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002592, whole genome shotgun sequence.
ACCESSION   DSEB01000473 DSEB01000000
VERSION     DSEB01000473.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002592"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..353)
                     /locus_tag="ENO07_08455"
     CDS             complement(<1..353)
                     /locus_tag="ENO07_08455"
                     /inference="COORDINATES: protein motif:HMM:PF02190.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDS02035.1"
                     /translation="MDGRMQMVKSELKKNIKEVTLLPLKGMVVFPYLVMPLMISNQKY
                     AKMVDEALLEGKTIGLFTQKPGESADENEDNIYRIGTSASILKMLRFPDGSVRFLVQG
                     LSRIRLRKIISSEPYL"
     gene            complement(424..2391)
                     /locus_tag="ENO07_08460"
     CDS             complement(424..2391)
                     /locus_tag="ENO07_08460"
                     /inference="COORDINATES: protein motif:HMM:PF13431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDS02036.1"
                     /translation="MDSKYKNKPLLIWLALFALVFALSPFLGHARLWGINHLKFLSDY
                     GKIIFIGLVILCFMPGIAGRINGGVNSIQSRYLKLKSIKKVLVSALIFIVSIGIFYLF
                     RNSTHLLGDGLLRGDELTYGFGYLPLSTEPLTSILHYLFYKIVNPLFGVQNHASIQIF
                     SCLLGGAFILLAINIFKPKKESGFSPLLAVIGISGIQFFFGYVESYIIPYIGLLIFVW
                     LSYKYFSTGKAFAAACIVYMIVFISHFSTIFTLPAFLVFLYFGLRNPELKPSHKITGI
                     ISLIVMGAVFVYFHYIYVPAFGHIEVGFLLSFTPDGYWILDPAHLLDILNNLLLSSTF
                     GLLLLPVIIKHKLWNNINIPARIFSFLLVCGSLGFLVFIDPKLGFARDWDLFVPAGAV
                     IAIIAVRMAHRAKEITADYRAEISIAVLLPMIFSASFIAVNQNLESSYRRFEHILQFH
                     AERSAFGYESLGGHYKRFYRNKEDLRRAIENYKRAFELLENPRYLTDIASVYNLYYNS
                     YTDQSISRRFIDSIEYYSKQALKYNDSLHSAFEYLTMASMYREDFERALEYSNRVLRF
                     VEKENEAEFRYKRAAIFLNMNDLRGAEKEFLKIIELDPGYSKAYVMLGHIYRQTGQNQ
                     KAIDYYSQYLQKFPDGEFRNEVEKILRNLRY"
     gene            complement(2439..3251)
                     /gene="pyrF"
                     /locus_tag="ENO07_08465"
     CDS             complement(2439..3251)
                     /gene="pyrF"
                     /locus_tag="ENO07_08465"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDS02037.1"
                     /translation="MKFKERISQLVKERKSLICVGLDTDPDKIPDHLKSTENPVLEFN
                     KLIIEATSDICVAYKPNSAFYEALGPNGMEILKATITAVPSEVPVIVDAKRGDIGNSS
                     RMYARAVFEYLEGDAVTVSPYLGEDSLAPFFEYKDKFAFVLALTSNPSSQDFQLRNVE
                     GKPLYEIVAENVQKWNKSDNLGLVVGASHPDKIKEMRNISGNLPFLIPGVGAQGGDLE
                     ASARFGTDNGKSIALINVSRSVIYASDGVDFAEKAREEVQRLNRELSEFEIF"
     gene            complement(3208..>3686)
                     /locus_tag="ENO07_08470"
     CDS             complement(3208..>3686)
                     /locus_tag="ENO07_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS02038.1"
                     /translation="PLIIKLSPNVTSIAEIARAAEEGGADCLSLINTLVGMGVNHWTF
                     KPILTNITGGLSGPAIKPVALAKVWQVYNAVKIPLIGIGGIMNHFDVAEFMIVGASLV
                     QFGTANFINPDCTVKAAEGLTDYLKQTGISDISELIGKLGGNHEIQGKNFPACQGT"
BASE COUNT          992 a          847 c          852 g          995 t
ORIGIN      
        1 agatagggct cactcgatat tattttcctg agccggatac gtgacagacc ctggaccaga
       61 aaacgcaccg aaccgtcagg aaaccgcagc atcttcaaaa ttgaggcgct ggtgccgatt
      121 ctgtatatat tgtcctcatt ttcgtcagca ctttcgccgg gtttctgggt gaataagccg
      181 attgttttcc cctccagaag agcctcatcg accatcttgg catatttctg gttgctgatc
      241 ataaggggca taaccaggta tgggaaaacg accataccct tgagcggcag aagtgtgact
      301 tcctttatat ttttcttaag ttcgcttttt accatctgca ttctaccatc catagcttta
      361 atgtataaag aaatcctttt ttcaatgtat agatacttaa tttaaccgga atgttccata
      421 gaattagtat cggagattcc gcagaatctt ttcaacttca tttctgaatt cgccgtcggg
      481 gaatttttgc agatactgag agtaataatc gatagccttc tgattttgtc cggtttgcct
      541 gtagatatgg ccgagcataa catatgcttt actgtacccg ggatcgagtt cgattatttt
      601 cagaaattct ttttctgctc cacgcaggtc attcatgttc aggaaaatgg ccgctcgttt
      661 atacctgaat tcggcctcat tctccttttc gacgaaacgc aggacccggt tgctgtattc
      721 cagcgccctt tcaaaatcct ctcgatacat gctcgccatg gtcaggtatt caaaagcgga
      781 atgcagtgaa tcattgtatt tcaaggcttg cttcgaataa tattctatgg agtcaataaa
      841 tcttctgctg atgctttgat cggtgtagct attgtagtag agattataga ccgaggcgat
      901 atccgtcaag tagcgtggat tctcaagaag ctcgaaagct cttttgtagt tttcgattgc
      961 cctgcgcaga tcttctttat tgcggtaaaa acgcttgtag tgaccgccca gtgattcata
     1021 tccgaaagcg cttcgctcgg catggaattg caggatatgt tcaaaacgcc gatagctgct
     1081 ttccagattc tggttgaccg ctataaatga ggctgagaag atcatgggta gcaggaccgc
     1141 tatgctgatt tcagcgcgat aatcggctgt aatttctttc gctctgtgcg ccatgcgaac
     1201 cgcgataatc gcaattaccg ccccggcagg cacaaacaga tcccagtccc gggcaaatcc
     1261 cagcttgggg tcaatgaaaa ccaggaagcc cagtgatccg caaacgagca gaaatgaaaa
     1321 tattcttgcg ggaatattga tattattcca gagcttatgc tttatgatga ctggtagcag
     1381 caatagccca aatgttgagg aaagaagtaa gttatttaat atatccagca ggtgtgcggg
     1441 atcgagaatc cagtagccat caggagtgaa tgatagaaga aatcccacct cgatatgacc
     1501 gaatgcagga acatagatat aatggaagta aacaaatact gcccccatta caattaatga
     1561 tataatcccg gtaattttat gcgatggttt aagctcggga tttcgcagac caaaatagag
     1621 gaatacaaga aatgcaggca atgtgaagat ggttgaaaaa tgagaaataa acactatcat
     1681 gtagactatg caggcagcgg cgaatgcttt tccggtcgag aaatatttgt agctgagcca
     1741 gacaaaaatc aggaggccga tgtaaggtat tatataactt tcgacataac cgaaaaagaa
     1801 ctggattccg ctgattccga taacagccag gagcggggag aatcctgatt cttttttggg
     1861 tttaaaaata ttgatagcca gtagaataaa ggcgccgccc agcaggcagc tgaaaatctg
     1921 tatgctggca tgattctgga cgccgaacag cgggttcact attttataaa acagatagtg
     1981 caggattgag gttaacggct cggtggataa aggcagataa ccgaaaccat aggtcagttc
     2041 atccccgcga agcaaaccat cgcccagtag atgagttgag ttgcggaata gatagaagat
     2101 cccgatcgaa actatgaata tcagtgctga gaccaggact tttttgatgc ttttaagctt
     2161 caaatacctg ctctgaatag aattaacccc gccattgatt ctgcccgcaa ttcccggcat
     2221 gaagcacaag ataacaagcc cgataaagat gattttgccg taatcgctga gaaatttgag
     2281 atggtttatt ccccacaggc gggcatggcc gagaaatggt gagagtgcga agacgagagc
     2341 aaataatgcc agccagatta acaggggctt gtttttatat tttgaatcca attatcttaa
     2401 tatctgctcc atcaagaaat tgagattatg cagcccaatc aaaatatctc gaactcggag
     2461 agttcgcggt tcaggcgctg tacctcctcg cgcgcctttt cggcgaagtc cacgccatcg
     2521 gatgcgtaga taaccgaacg gctgacattg atgagcgcga tcgattttcc attgtcggtg
     2581 ccgaacctgg cggatgcctc caggtcgcct ccctgggctc cgacccccgg gatcaggaag
     2641 ggaagattgc cggaaatgtt gcgcatctct ttgatcttgt ccggatgcga tgcccctacc
     2701 acaagaccga ggttatcact tttattccat ttctgcacat tttcagccac aatctcgtaa
     2761 agcggcttgc cttcaacatt tcgaagctgg aaatcctgag agctgggatt ggaagtcagc
     2821 gccagcacaa acgcaaattt gtccttatat tcgaaaaacg gtgccagcga atcctctccc
     2881 agatagggtg agactgtaac cgcatctcct tccaggtatt cgaagaccgc cctggcatac
     2941 atgcgcgagc tgttgccgat gtcgccgcgt ttggcatcca caattacggg gacctccgaa
     3001 gggacggccg taattgtcgc cttcagtatt tccatcccat tcggccccag cgcttcgtag
     3061 aaagcggagt ttggcttata cgccacgcaa atgtcggagg tagcctcgat gataagtttg
     3121 ttgaattcca gtacgggatt ttctgtcgat ttcagatgat ctggaatttt atccgggtcg
     3181 gtgtcaagcc cgacacatat caggctctta cgttccttga caagctggga aattctttcc
     3241 ttgaatttca tgattaccac ccaattttcc tattaattcg gatatatcag atatacccgt
     3301 ttgttttaaa taatcggtca gaccttcggc agcttttacc gtgcagtcgg gatttatgaa
     3361 attggccgtt ccgaactgaa ccagcgaggc tcccacgatc atgaattcgg ccacatcaaa
     3421 atggttcatt atcccgccaa ttccgatcaa cgggatcttc acagcattat agacctgcca
     3481 tacctttgcc agggctacgg gttttattgc cggacccgat aatcctccgg taatattggt
     3541 taaaatcggc ttgaaagtcc aatgatttac tcccatgccg accagagtgt taatgagcga
     3601 gagacaatcg gctccgcctt cctcggccgc tctggcgatc tccgctatgc tggtgacatt
     3661 gggagaaagc tttattatca gcggat
//
LOCUS       DSEB01000474            3679 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002612, whole genome shotgun sequence.
ACCESSION   DSEB01000474 DSEB01000000
VERSION     DSEB01000474.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002612"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..2607
                     /gene="mutS"
                     /locus_tag="ENO07_08475"
     CDS             <1..2607
                     /gene="mutS"
                     /locus_tag="ENO07_08475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDS02039.1"
                     /translation="NYMANDKLTPLMEQYYRIKKDYPDKILFFRMGDFYEMFGEDAEE
                     AAPILEIALTSRAHGKAAPKVPLAGIPYHALEKYLGRLIEAGKKVVICEQTEDPKLAK
                     GLVKREVVEVITPGTYTSGADNGNRFNYLAAVVNTGNSYGLSYLELSTGDFYLDDVAG
                     DDLIDRLRTLDPKEIVIDDADGHLKSKIKLYLPAAAVSKLDSWKFDRQTAHQKLVDHF
                     KVDNLEGFGIDNGNAGICSAGGILYYLDENKLIDLKHIRAVRIPHFEENMFLDSSTLE
                     NLEIFSSPSGKSLFTILNRTRTSMGARRLRQALQAPLRNLQKIKTRQQNLTRLVEDRQ
                     LLKDMSELMKSISDLERISGRLGRAKAGPRDIYTLQVSLEATAKLRELTKALDFPEYA
                     ITEEDMVELERIAEQISAAIVDEPPFGYHEGGIFKAGYDQALDDLKESISESQRWIAG
                     LQAEEKGRTGIPSLKVGFNKVFGYYIEVTRPHLEKVPPNYIRKQTLVSAERFITEELK
                     QKEEIILSAEEKINRLEENLFLQLCEELSLKIELIQKAAHFVAEADLSASLASCAISF
                     NYIRPELNNGDRIKIINGRHPVIEQILPSGKFVPNDTEVDTKKSQMHIITGPNMAGKS
                     TYLRQVGQIVLLAQTGSYIPAESAEIGLVDRIFTRVGATDKITLGQSTFLVEMNETAN
                     ILHNCTEKSLILLDEIGRGTSTYDGLSIAWAVSEYLHETDGKQAKTLFATHYHELTRL
                     ANRFKRIKNFQVAVKEWQDQIIFIRKIVPGGCDDSYGIFVAQLAGVPKEVTDRARTIL
                     RLLEGGKFLSDIKSDSEKESASLFEHKYGKTNHHYEKIIEKLKSLNPEQTTPLEALNI
                     LVKLKEMIGDDD"
     gene            2604..>3679
                     /gene="mutL"
                     /locus_tag="ENO07_08480"
     CDS             2604..>3679
                     /gene="mutL"
                     /locus_tag="ENO07_08480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDS02040.1"
                     /translation="MRQIAVLPEILINKIAAGEVVERPSSVVKELVENSLDAGATRIK
                     VEIEKGGSKQIKVTDNGEGIAGDFLPVALQRHATSKLQSLEELDSISSLGFRGEALPS
                     IASVSLFEITSKTKDQLSGFRIKVEGGRIVEQTDIGAPDGTMIEVRELFFNTPARRKF
                     LKTQVTETRHIIDAINGLALAYPHCGFELVSDGRQLFDYNIAGDYKSRVKDVLGGDFD
                     KMVEFSDKGDRISLSGFTIKPEFALKNRAQIYFIVNGRRISSPLLYSALMRAYGEFLI
                     KGRYPQAVIYIFMDPSALDINVSPTKSEVRFSDERSIYGALMSAVRKCITSHDVIPSG
                     FGSVKPGFGVEEENESADYRERIRA"
BASE COUNT          981 a          861 c         1019 g          818 t
ORIGIN      
        1 aattatatgg caaatgacaa attgacaccc ttaatggagc aatattaccg gatcaagaag
       61 gattatccgg acaagatact cttcttccgc atgggcgatt tttatgagat gttcggggag
      121 gatgccgaag aggctgcgcc catcctggag atcgccctga cttcccgcgc gcatggcaaa
      181 gcggcgccca aagtgccgct ggcggggata ccgtatcatg cgctggaaaa atatctgggg
      241 cgcctgatcg aagccggcaa aaaagttgtc atctgcgagc agaccgaaga cccgaaactg
      301 gccaaaggtc tggtgaagcg cgaggtggtg gaggtgatca ctcccggaac gtacacctcc
      361 ggagctgata acggcaaccg cttcaactac ctggcggctg tcgtcaatac tggtaacagt
      421 tatggcctca gctacctgga actttctacc ggggattttt acctggatga tgtggccggg
      481 gatgatctta tcgaccgtct gcgcacgctc gatccgaagg agattgtaat cgacgatgcc
      541 gatgggcatc ttaaatccaa aataaaatta tatctgcccg ctgcggccgt gtccaaactg
      601 gattcgtgga aatttgaccg ccagaccgcc catcagaaat tagtcgatca tttcaaagtc
      661 gataacctgg agggcttcgg gatcgataat ggtaatgcag ggatatgttc ggcgggagga
      721 atactgtatt atctcgatga aaacaagttg atagatttga aacatatacg ggcagtgagg
      781 attccccatt ttgaggaaaa tatgtttctg gattcctcca cgctcgagaa tctggaaata
      841 ttctcctcgc cctccggcaa gtcgcttttc acaatactca atcgcaccag gacctcgatg
      901 ggtgcgcgcc gtctgcgtca ggcgctgcag gcgccattaa gaaatctgca gaagatcaag
      961 acccgccagc aaaatctgac caggctggtc gaagacagac agcttttgaa ggatatgtcg
     1021 gaattgatga aatcgatctc cgatctggag cggatttcag gaagactggg gcgcgccaaa
     1081 gcgggaccaa gggatattta tactcttcag gtttcgctcg aagccacagc gaaattgaga
     1141 gaactgacaa aagcacttga ttttccggag tatgcgatta cggaagaaga tatggttgag
     1201 ctggaacgga ttgcggagca gatcagcgcc gccattgttg acgagccgcc gtttggctac
     1261 catgagggcg ggattttcaa agcggggtat gatcaggcgc tggatgattt gaaggagtcg
     1321 atttctgaat cgcagagatg gatcgcgggc ctgcaggctg aggagaaggg acgtaccggg
     1381 ataccctcgc tcaaggtcgg ttttaacaag gtcttcgggt attacataga ggtcaccagg
     1441 ccgcatctgg agaaagtgcc gccgaactat attcgtaaac agacactggt atcagccgag
     1501 cgttttatca ccgaggagct gaagcagaaa gaagagatta ttctgtcggc cgaggagaag
     1561 attaaccgcc ttgaggaaaa cctgtttctt caattgtgcg aggaattgtc gcttaaaata
     1621 gagctgatcc agaaagctgc gcattttgtg gccgaggccg acctttcagc atctctggca
     1681 tcatgcgcca tcagcttcaa ttatataagg cccgagttga acaacggcga cagaataaag
     1741 ataataaacg ggaggcaccc ggtcatagag cagatccttc cctccggtaa attcgtgccc
     1801 aatgatacag aggtggatac caaaaaaagc cagatgcata taatcaccgg ccccaatatg
     1861 gccggcaaat cgacatatct gcggcaggtg gggcagatag tcctgctggc gcagaccgga
     1921 tcatacatcc cggccgaatc ggccgagatc gggttagtgg acaggatttt taccagggtc
     1981 ggcgcgacag acaaaattac cctggggcag tcgaccttcc tggtggagat gaatgaaacc
     2041 gccaatatcc tgcataactg cacggagaag agtctgattt tgctggatga aatcggtaga
     2101 ggaacatcga cctatgacgg cctctcgatc gcctgggcgg tcagcgagta tctgcatgag
     2161 accgatggta agcaggccaa gaccctgttt gccacacatt atcatgaact gacccgcctg
     2221 gccaacaggt tcaagcgcat caaaaacttc caggtggcgg tcaaggaatg gcaggaccaa
     2281 attattttca tccgcaaaat tgttccgggc ggctgtgacg acagctacgg tatctttgtg
     2341 gcccagcttg ccggcgtacc caaagaggtg accgaccggg caaggacaat tctacgtctg
     2401 ctggagggcg gcaagttttt gtcggatata aaatcagatt ctgaaaaaga aagcgcaagc
     2461 ctgttcgaac ataaatacgg caaaacgaac caccactacg agaagattat cgagaagctc
     2521 aaatccctca atccggaaca aaccacgccg ctggaagcat tgaatatact tgtaaagctc
     2581 aaagagatga ttggagatga cgattgagac agattgcagt tcttccggaa atcctgatta
     2641 ataaaatcgc tgccggcgag gtggtggaac ggccatcctc ggtggtcaaa gaactcgtgg
     2701 agaactccct ggatgccggc gcgaccagga ttaaggttga gattgaaaag ggcggaagca
     2761 agcagattaa ggtcactgat aatggtgagg gtattgccgg cgattttctg ccggtagcat
     2821 tgcagcgtca cgctacctcc aagctgcagt cgctggagga gctggattca atttccagcc
     2881 ttggatttcg gggggaggcg ctgccgtcga tagcatcggt ctcattattc gaaatcactt
     2941 ccaagaccaa agaccagctt tcaggcttta gaatcaaggt cgaaggcggc aggatcgtcg
     3001 agcagaccga catcggtgca cccgacggca ccatgataga ggttcgtgag ctttttttca
     3061 atacccccgc caggagaaaa tttctcaaga cccaggtaac cgagacacgt catattatcg
     3121 acgccataaa tggcctggca ttggcatatc cgcattgcgg ctttgagctt gtcagcgacg
     3181 gacgccaatt atttgactac aatattgctg gtgattacaa aagcagggtg aaagatgtgc
     3241 tggggggcga tttcgataag atggtggagt tttcggataa gggcgataga atcagccttt
     3301 ccggatttac catcaaaccc gagtttgcac tgaaaaaccg ggcccagatt tatttcatcg
     3361 tcaacgggcg gcgcatcagc tcgccgcttt tgtattctgc tctgatgagg gcctacgggg
     3421 aattcctgat caagggacgc tatccgcagg cggttatata tatcttcatg gatccatcgg
     3481 cgctggatat caatgtcagc cccaccaaaa gcgaggttcg tttttccgac gagcgttcta
     3541 tctatggcgc attgatgtcg gcggtgagga aatgtattac cagccatgat gttatcccgt
     3601 caggcttcgg ctcggtgaag cccggatttg gggttgaaga ggagaacgaa tctgcagatt
     3661 acagagaacg gatcagggc
//
LOCUS       DSEB01000475            2990 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004151, whole genome shotgun sequence.
ACCESSION   DSEB01000475 DSEB01000000
VERSION     DSEB01000475.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004151"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(86..877)
                     /locus_tag="ENO07_08485"
     CDS             complement(86..877)
                     /locus_tag="ENO07_08485"
                     /inference="COORDINATES: protein motif:HMM:PF06082.9"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS02041.1"
                     /translation="MPKRPVTFLIGLFMFFAANNYAQDADLNPLPDVASPDKLVDYPT
                     AGTLPRASFNVELDAFSRGGILSFINIGLHERFTLGISYGGTGILGDIDPDWHKRPEY
                     KIKLRLIDESYAFPAIAIGFESQGTGIYDDELDRYQFKAPGFYLVASKGYRAYNWVSG
                     IHLGAHLNTIEDNRDGDDDPSFFAGFDITFNNNLSITGEYQAALNDNSESSRYGRGRG
                     YMNMGVLWTFSDQLQLGAIFKDLLNNSRVTESITRELRITYTERF"
     gene            complement(1110..1622)
                     /locus_tag="ENO07_08490"
     CDS             complement(1110..1622)
                     /locus_tag="ENO07_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDS02042.1"
                     /translation="MDFFDVVNKRRSIRAYENKPVEEDKLRQILKAGRKAPSANNKQN
                     WKFIVVRDKFKIEKLTYACKQQNFVSEAPVLIIGCALNTDYKMTCGQLAYPIDTSIAL
                     THMMLAATALDLGTCWLGAFYEDKVRKILRIPEDVRVVGILTLGYPRFQPSETNRKNL
                     SEIVSFDKWG"
     gene            1633..2955
                     /locus_tag="ENO07_08495"
     CDS             1633..2955
                     /locus_tag="ENO07_08495"
                     /inference="COORDINATES: protein motif:HMM:PF04165.10"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HDS02043.1"
                     /translation="MLILYPQHKLSKPPPLSTVFLYFAGFKFKHYVYLRAMEIAKLAV
                     VFGIIILLLRIRLSVGVSIFLGGIALGFLFGEHFFEIGKSVLGGMVAWRTIRLILIVS
                     TISTLGTLLKNLEITGRLVSGIENLSGSIKVTLVLCPVIIGLMPMPGGALLSAPLVAA
                     GAEGQPISKARLSAINYWFRHIFEFCWPIYPGIILAAAILELDISQISLWQAPFSIPF
                     ILGGLIFLIFPMKNVSRFTRDQSLRASWLNVLSGVWPILAVVFITLVFGADLLLSLAI
                     AFALFLVLVRPPLSLTLRSVKEGCSFAIISLMFGVMVFQAIISDSGAAVDIAHRIASW
                     GVPDWAVVSLSCFLIGFVAGIVTAYVGIVYPILIPILMAPEPKISMIVLAYASGLIGV
                     MTSPLHLCLILTKEYFRSSLKRIYPLILGPALFVAISVAIILALGYGK"
BASE COUNT          678 a          733 c          722 g          857 t
ORIGIN      
        1 ataacagcct tgcccaatta tcagcaaagc gcctattatt gcgattgtga aaactgcttt
       61 ttttgccatg atcatcacct gctctctaaa atctctccgt atatgtaata cgcagctctc
      121 tggtaattga ctcagttact ctgctattat ttaagagatc tttgaatatc gcgcccagtt
      181 gcagttgatc cgagaacgtc cacagcacac ccatgttcat ataaccgcgt cccctgccat
      241 agcgggaact ctcagaatta tcatttagag ccgcctgata ttcgcccgtg atagagagat
      301 tattgttaaa agttatgtca aatcccgcaa agaacgaggg atcatcatca ccgtccctgt
      361 tgtcttcaat tgtatttaaa tgagcgccca gatgaatacc ggaaacccag ttataggccc
      421 tgtacccttt ggacgcgacc agatagaagc cgggcgcctt gaactgataa cggtccagct
      481 catcatcgta aattccggta ccctgggatt caaatccgat ggctatggcc ggaaaggcgt
      541 agctttcatc gatcagccgc agtttgattt tatactccgg acgcttatgc cagtctggat
      601 caatgtcccc cagaataccg gtcccgccat aagatattcc aagggtaaag cgttcatgca
      661 ggccgatatt gatgaaactc agtatgcctc cccgtgagaa ggcatccagc tcgacattaa
      721 atgacgctct cggaagtgtt ccagccgtgg ggtagtccac cagtttgtca gggctggcaa
      781 cgtccggcag cggattcagg tcagcatcct gtgcatagtt gttagctgcg aaaaacataa
      841 atagtccgat caaaaaagta actggcctct tcggcatcct gcctcctatc ctgtctattt
      901 tttcataaga aaaaattcat aaacaggacg gtcaacataa aactccccgt ctgtattcaa
      961 ctcatgcaaa acgcttgccg ttattgatgc tattatgcgc ccgtaagtcg atgtattcaa
     1021 tggtgatatg gaatgctacc atgagatgac gggcgaggct cccttttctg cacaattatt
     1081 gggcagggaa ctgcttgaat gcacaataat tagccccatt tatcgaatga gacgatctcg
     1141 cttagatttt ttctgttggt ttctgagggt tggaatctgg ggtagccaag ggtcaatatt
     1201 cctaccactc gaacatcctc aggaatcctc aggatttttc tgactttgtc ctcataaaat
     1261 gcgcccagcc agcatgttcc caggtcgagc gcggtggccg ccagcatcat atgggtaagc
     1321 gcaatggatg tgtctatggg atacgccagt tgcccgcagg tcatcttata atcggtgttt
     1381 aaggcgcatc caattatcag caccggagcc tcactgacga agttctgctg cttgcaggca
     1441 taggtcagct tctcaatctt gaacttatcc ctgaccacaa tgaacttcca gttctgcttg
     1501 ttgttggcgg aaggggcctt gcggccggcc ttcaggatct gccgcagttt atcttcctcc
     1561 acaggtttgt tttcgtaggc cctgatcgaa cgccgtttat tgaccacatc aaagaaatcc
     1621 attaaattct ccatgctgat attatatcca cagcataagc tatcaaaacc gccgcccctg
     1681 tcaactgttt tcctttattt tgctggattt aagtttaagc attatgtata cttacgggct
     1741 atggaaatcg ccaagcttgc agtcgttttc ggaattatca tcctgctctt gagaataagg
     1801 ctttcggtgg gcgtttccat atttctggga gggattgcgc tcgggttcct gttcggagag
     1861 cattttttcg agataggaaa atcggtactg ggaggaatgg tcgcctggcg cactattcga
     1921 cttatactga tagtcagcac catcagcacc ctggggacgc tcctaaaaaa tctggagatc
     1981 accggccggc tggtttcggg gatagagaat ttatcgggat cgatcaaggt gacgctggtt
     2041 ctatgcccgg taattatagg tctgatgcct atgcccgggg gggcgcttct ctcggctccg
     2101 ctggtggcag cgggagccga ggggcaacct atcagtaaag ctcgtctctc ggcgataaat
     2161 tactggttca ggcacatatt cgaattctgc tggccgattt atccgggtat cattttggcc
     2221 gcagcaattc tggagctgga tatttctcaa atctccttat ggcaggcacc gttctcgatt
     2281 cccttcatac tgggggggct gatttttctt atatttccca tgaaaaatgt gtccagattt
     2341 acacgggatc aatccctgag agcgagctgg ctgaatgtat tgtcaggggt ctggccgatc
     2401 ctggcggtgg tttttattac ccttgtcttt ggtgccgatc ttctgctcag cctggccata
     2461 gcgtttgcac tcttcctggt ccttgtgagg ccgccattga gcctcacgtt gcgctctgtt
     2521 aaggagggtt gctccttcgc catcatctcg ctgatgttcg gtgtcatggt gtttcaggca
     2581 atcataagcg attccggggc ggcagtggat attgcccata gaatcgcctc ttggggcgtg
     2641 ccggactggg cggttgtatc cctttcatgt tttctgatag gatttgtggc cggtatcgta
     2701 accgcttatg tgggaatcgt ataccccatc ctgatcccta ttttaatggc tcccgagccg
     2761 aaaatctcga tgatagtcct ggcctatgcc agcgggctta ttggggttat gacttcgccc
     2821 ctgcatctgt gccttatcct gaccaaggag tatttcaggt cgagcctcaa gaggatttat
     2881 ccgctcatac tggggcccgc attatttgtg gcgatttcgg tggcaatcat actggcgctg
     2941 ggctacggca aataattgat atattgtttg cgtattcagg ataaattttg
//
LOCUS       DSEB01000476            3140 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003740, whole genome shotgun sequence.
ACCESSION   DSEB01000476 DSEB01000000
VERSION     DSEB01000476.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003740"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            477..1265
                     /locus_tag="ENO07_08500"
     CDS             477..1265
                     /locus_tag="ENO07_08500"
                     /inference="COORDINATES: protein motif:HMM:PF12697.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDS02044.1"
                     /translation="MIESRVRKYGSPPYRAAVLHGGPGAPGYMAPVARELSQKIGVLE
                     PLQTRDSVDGQIEELKSQLVEHSDKPVTLIGSSWGAVLALFLASRYPQLNDKLILIGS
                     AVFDAESSARIERIRTERLNEKERKRLHEIESQLLTAAPDEKVVLFEELGDLFTKTDK
                     YDPIDADDETIEVQHEIFNKVWPEFVKLRDRPGYLKKEFSRINIPVTVIHGDYDPHPL
                     EGIKPFLEACISDTNFYLLPGCGHYPWLERHARERFFEILFSEI"
     gene            complement(1352..2071)
                     /locus_tag="ENO07_08505"
     CDS             complement(1352..2071)
                     /locus_tag="ENO07_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS02045.1"
                     /translation="MYSSIPNRQGRNVLSIIVSIFLLLAGGSILMFVACGGEETIADV
                     TVYEAQIKPLNENVTGYQPRGSARLEILGDSLTIIVNVSNLPPGMMHLQHYHGFVDGS
                     EAGCPTMKQDANQDSIIDLIETQTVAGITLVPFHHDPLSLEIAAEIYPSADSLGYLSY
                     QATISMDSLENRMAGKYGITPLVFENRVVFMHGVDSATALPETVQSLEGVPAHLTLPI
                     ACGKFELRVFDEDVASEGIYQ"
     gene            2332..>3140
                     /locus_tag="ENO07_08510"
     CDS             2332..>3140
                     /locus_tag="ENO07_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS02046.1"
                     /translation="MKKLMVAILLLGFILPGTVLALDYLETDQSKYIDTPGFLGFVPD
                     RFIVVLKDNVAVDHGKDLNRSVALSNLSGFDALAQKYEVESLRPQFPGADRNSVSLTA
                     EAQDISRHYKVYIQSGTLEEAMADYAALPDVERVEPIGVHVLYSTPNDPYYVDPPSGY
                     AYDQWHYWDTYGMNADSAWDKEAGDNTVLVGVLDMGAKYDHIDLGGSNPPGPNDASTN
                     GNIWVNTNEIPGNGVDDDGNGYVDDIIGWDFVESTDWYSYPCVDLDCGGAD"
BASE COUNT          860 a          711 c          754 g          815 t
ORIGIN      
        1 actatacgct gcggattaaa tatcaattcc aggaggacct ttataattta aaagtcaccg
       61 atgaatatat tcgcctcatc ccggtagata caaatttcac cgaacctcgc tatgaattat
      121 actggcgcta tgtccccgga tcatttgcat atatctgtaa agccagcaca gagatgtcat
      181 atctttgcga ttattttgcg gacacccttt tgagcgaaat tgacctggag gaattttatt
      241 atcccgattc cggttggata ccctacgaaa gaagctggaa cgataagcat gtacggtact
      301 tcatatatga gagcgaatcc gattatgacc atgcggttga aatcctggaa gcatttactc
      361 acgaccacat tatgcaacgg gaaatccgaa taactctcag gaactggatg agcgagaggt
      421 atggctcgtt taattttgat aactggaaat aggtcgttta ttataagttt aactttgtga
      481 ttgaatccag agtccgaaaa tacggttctc cgccatacag ggcagccgtc cttcatggcg
      541 gccccggagc gccgggatat atggcgccgg tggcccgtga attatcacag aaaattggag
      601 tcctcgaacc gctgcagacc agagattcgg ttgacggcca gattgaagaa ctgaaaagcc
      661 agcttgtgga acattctgac aagcctgtga cattgatagg ctcctcatgg ggcgcggtgc
      721 tggcgctttt tctggcttcc agatatccac agcttaatga taaattgatt ttaatcggca
      781 gcgcagtctt cgatgccgag agttcagcca gaatagaaag aatcagaacc gaacgcttga
      841 atgagaaaga acgaaagcgc ctgcatgaaa tcgaaagcca acttctgact gctgctcctg
      901 atgaaaaagt agtactcttt gaagagttag gtgacttatt tacaaaaact gacaaatatg
      961 atccaatcga tgcggatgat gagactatcg aggtgcagca tgagatcttt aacaaagtct
     1021 ggccggaatt cgtgaagctg cgcgacaggc ccggctatct aaaaaaggaa ttctccagaa
     1081 ttaatattcc cgtcacggtc attcacggag attacgaccc gcatccactg gaaggaatca
     1141 agccttttct cgaggcttgc atttctgaca caaattttta cctcctgcca ggatgcgggc
     1201 attacccctg gctggagcgt catgccagag agcgtttttt cgagatactt ttcagtgaga
     1261 tatgatcggt cacggaaaaa cagaataaga atagggccaa tccggatcac ggattggcct
     1321 gtttttattg tcgcaaaatt tcaatctgct tttactgata aatgccttcg ctggctacgt
     1381 cctcgtcgaa tacgcgcagc tcaaatttgc cgcaggcgat gggcaaggta aggtgagccg
     1441 ggactccttc cagcgactgt actgtttcgg gcagggcggt ggctgaatca acgccatgca
     1501 tgaaaactac cctgttttcg aataccagcg gagtaatacc gtatttgcct gccattctgt
     1561 tctcgagtga atccatggaa attgtggcct gataagagag ataacccagg gaatctgcag
     1621 acggatatat ttccgcggca atctccaggc tgagaggatc atgatgaaac ggaaccaggg
     1681 ttattcccgc aactgtctgt gtctctatta aatcgataat actgtcctga tttgcatcct
     1741 gcttcattgt cgggcagccc gcctcggatc cgtcaacaaa gccgtgatag tgctggagat
     1801 gcatcattcc cggaggaaga ttggagacat tgacgataat ggtaagcgaa tcgccgagaa
     1861 tttccagtcg agcgcttccc cgaggttgat atccggtcac attttcattc aaaggtttga
     1921 tctgagcttc ataaactgta acatctgcaa tcgtctcctc cccgccgcag gccacaaaca
     1981 tgagtatcga tccgcctgca agaagcagga atattgagac gataattgac agtacattcc
     2041 tgccttgtcg attcgggatt gagctgtaca taaaacctcc tctataatac tacccgccct
     2101 ttcaattatt tatctctttg ctatgcataa caatacgggg aaattgatgt tccgggcaat
     2161 cattttagag attttggaat cagttatcat aatctcttgc aaatttttca ataatatata
     2221 tatttatata aatccccgct ttcaggatga aaagattact atctgttcgg caatagaaaa
     2281 aatattagca gcattgaaac ttgaactttt tcagcaaagg aggagaaacg tatgaaaaag
     2341 ttgatggtgg ctatcttgtt gcttgggttc atcttgcccg gaacagtcct ggcactggac
     2401 tacctggaaa cggatcaatc caagtacatt gacaccccgg gatttctcgg atttgtcccg
     2461 gaccgcttca ttgttgtttt aaaagacaat gtggctgtcg atcatggaaa agacttgaac
     2521 agatcagtag ccctgtccaa cctctcgggc tttgatgcac tggctcagaa atacgaagtc
     2581 gagagcttaa ggccccagtt ccccggtgcc gaccgcaaca gtgtatcgct cacagcggaa
     2641 gcacaggata tttcacggca ttacaaagtt tacattcaga gtggaacttt ggaagaagcg
     2701 atggccgatt atgccgccct cccggacgtg gaaagggttg agccgatcgg ggtccatgtt
     2761 ctctattcca ctcccaatga cccatattat gtggatcctc ccagcggata tgcctatgac
     2821 cagtggcatt actgggacac ttatggtatg aatgccgaca gcgcctggga taaggaagcc
     2881 ggcgataata cggtactggt gggcgtcctc gatatgggcg caaaatatga ccatatcgat
     2941 ctgggcggga gtaatcctcc cggtccgaac gatgccagta caaacggtaa tatctgggtt
     3001 aatacgaacg agattcccgg aaatggcgtc gatgatgacg gcaatggcta tgtcgacgac
     3061 attattggat gggactttgt agagagtacc gattggtatt catatccctg tgtcgacctc
     3121 gattgcggag gggccgacaa
//
LOCUS       DSEB01000477            2987 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004159, whole genome shotgun sequence.
ACCESSION   DSEB01000477 DSEB01000000
VERSION     DSEB01000477.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004159"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            2..517
                     /locus_tag="ENO07_08515"
     CDS             2..517
                     /locus_tag="ENO07_08515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDS02047.1"
                     /translation="MCILGFEIDFNLKIKECIVMQISTKGRYGLRAVLELARSSGDKP
                     VSTKEISQKQRITIPYLEQLFFRLKHKGFVKSVRGAQGGYFLAKDPEDISVGDIIECL
                     DGPIEFTDCKATSDGHSTCIGPENCMAISFWDDLKASLNEKLYGTSLADLLDDFKKKK
                     MRMKEGIETEA"
     gene            517..1683
                     /locus_tag="ENO07_08520"
     CDS             517..1683
                     /locus_tag="ENO07_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDS02048.1"
                     /translation="MLDKLIYLDHNSTTPPDPRVIKAMLPYYTEAWGNASSLHQFGRH
                     AKAGIEEAREIIAGAFNCLPEEIFFTSGGTESDNFAVKGTAYEHRSKGRHIITSKIEH
                     HAILESFAFLEEDGFEATYLPVDKYGFVDPDDLRKAIRSDTILVSIMYANNEVGTIQR
                     MDELAWICAEKGVIFHSDAVQAAGKIPIDLKKTPISLMSTSGHKIYGPKGVGILYVRQ
                     GTRIMRWQTGGHHERGLRAGTENVPAIVGYGKAMGLARAEMRENDRKISGMAEEFRRR
                     VLAEIPDVRFNGPENDRIKGTVSLCFKGAEGEAIILNLDMTGIGVTSGSACTSGALEP
                     SHVLTAMGVPPELAQSAIRFSFGKDNDPGDIDYIINSLKAAIEKLRMMSPFYQS"
     gene            1753..2847
                     /gene="mnmA"
                     /locus_tag="ENO07_08525"
     CDS             1753..2847
                     /gene="mnmA"
                     /locus_tag="ENO07_08525"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDS02049.1"
                     /translation="MVKKTVAVAMSGGVDSSLSAILLQQQGFNVFGVTMKLWDYADVG
                     GGTNTNSGCCNINLIEGARAVCAKYNIPHHVLNLSEDFKESVIADFVEEYKKGRTPNP
                     CVVCNTRIKWDVLLDKVQVMGADFLATGHYAQVEFYPRSGRWVLKKGLDQKRDQSYFL
                     WGLNQDSLSRTLFPIGKMTKKRVRQLAREYELRTAENPESREICFVTDNNYRRFLKDY
                     AKLEPKPGPILDEEGNKVGEHSGVQNYTVGQRRGLNIALGEPVYVKKIDPENNVIQVG
                     RAQSIKASGFIVNDMNWVSISEPDKTIECVVKIRYTPREFPVKLAVLDNHECEVDLSE
                     PVASITPGQSAVFYNQDVVLSGGIIKEVKT"
BASE COUNT          799 a          647 c          808 g          733 t
ORIGIN      
        1 aatttgtata ttggggttcg aaattgattt taatttaaaa ataaaagagt gtattgtgat
       61 gcaaatttca accaaaggca gatacggttt aagggcggtc ctggagctgg cccggagttc
      121 aggcgataag cctgtttcca ccaaggaaat atcccagaaa cagagaatta ccatccctta
      181 cctggagcag ctcttcttcc ggctcaagca taaggggttt gtgaaatcgg tcaggggggc
      241 ccagggcgga tactttctgg caaaggaccc ggaggatatt tcagtcggag atatcatcga
      301 atgtctcgat ggcccgatcg agtttactga ttgcaaggct accagtgacg gccactcgac
      361 ctgcatcggt cctgaaaact gtatggccat ttctttctgg gacgatttaa aagcctcgct
      421 gaatgaaaag ctctacggta cctcattggc cgatctgctt gatgatttca aaaagaagaa
      481 aatgagaatg aaagaaggca ttgaaacgga ggcctgatat tggataagct tatatatctg
      541 gatcataaca gcaccacgcc tcccgacccg cgagtaataa aggctatgct gccttactat
      601 actgaagcct ggggtaatgc atccagcctg caccagttcg gcaggcacgc taaggcaggt
      661 attgaggagg cccgggaaat catagccggg gcttttaact gtttaccgga agagatcttc
      721 tttacttcgg gcggaactga atccgataat tttgccgtga aaggaacggc ttatgagcat
      781 agaagtaaag gccggcatat aataacatca aagatagagc atcatgcaat cctcgaatct
      841 ttcgctttcc tggaagagga tggcttcgag gcgacctatc ttcccgttga caagtacggt
      901 tttgtcgatc ccgatgattt gaggaaagcg attcgatcgg atacgatcct ggtatcgatc
      961 atgtacgcca acaatgaggt cgggactatt cagagaatgg atgaactggc ctggatatgt
     1021 gcggagaagg gagtgatttt ccactccgat gctgtgcagg cggccggcaa gataccgata
     1081 gatctcaaga aaactccgat ttcgttgatg tccacttcgg gccataagat ttatggtccc
     1141 aagggcgtcg ggattctgta tgtccggcag ggcaccagaa tcatgcgctg gcagaccggc
     1201 ggacatcatg agcgcggact gcgtgccggt acggaaaatg tccctgcgat cgtcggttat
     1261 gggaaagcta tgggactggc ccgggccgag atgcgggaga acgatagaaa gatttcaggt
     1321 atggccgagg aattccgccg gagagttttg gcggagattc ccgatgtcag atttaacggg
     1381 ccggagaatg atcgtattaa gggcactgtc agtttgtgtt tcaaaggggc cgagggcgag
     1441 gccatcatcc tgaatctgga tatgacggga atcggagtga cctccggctc ggcatgtacc
     1501 tcgggagctc tggaaccctc acatgtgctt acagccatgg gagttcctcc ggaactggcg
     1561 cagtcggcta tacgtttctc ttttggcaag gataacgatc caggtgatat tgattacata
     1621 ataaattctt tgaaggcggc cattgagaaa ctcagaatga tgtcgccctt ctaccaatcg
     1681 taataaaagc cagggcttga ttggtgaacg ctctgaatag aaagcgtttg ccgtatcttt
     1741 caggattata aaatggttaa gaaaacggtg gctgtagcaa tgtccggggg tgtcgattcc
     1801 tcgctctcgg cgattttact tcaacagcag ggcttcaatg tctttggggt gactatgaag
     1861 ctctgggatt atgctgatgt gggcgggggc accaacacca attccggatg ctgcaatatt
     1921 aatctgatcg agggggccag ggcggtttgc gcaaaataca acattcccca tcatgtttta
     1981 aatttatccg aggatttcaa ggaatcggtt atagccgatt ttgtagagga atataaaaag
     2041 ggacgcacgc ctaatccctg tgtggtctgc aatacccgaa taaaatggga tgtccttttg
     2101 gacaaagtgc aggtcatggg cgccgatttt ttggcgacgg gccattatgc gcaggtggaa
     2161 ttctatccca ggtccggccg ctgggttctc aaaaaaggtc tggatcaaaa gcgtgaccag
     2221 tcttatttcc tctgggggct gaaccaggat tctctctcaa ggactctttt cccgatcggc
     2281 aagatgacca aaaagcgtgt gcgtcaactg gcccgcgaat acgaacttcg aaccgctgaa
     2341 aatcccgagt cgcgggaaat ctgctttgtc accgataaca actaccgccg ctttttgaaa
     2401 gactatgcca agcttgagcc gaaaccgggg ccgatcctcg atgaagaggg aaataaggtg
     2461 ggagagcatt caggggttca gaattatacg gtgggccaga gacgcggcct gaatattgcc
     2521 ctcggtgagc ctgtgtatgt aaagaagatc gatccggaga ataatgtcat tcaggttggc
     2581 agggcgcaga gtattaaagc ctccggcttt atcgtcaatg atatgaattg ggtctctatt
     2641 tccgagccgg ataagacaat tgaatgcgtg gtgaaaatca ggtacactcc ccgtgaattt
     2701 ccggtcaaac tcgctgtgct cgataatcat gaatgcgaag tcgatctatc cgagccggtg
     2761 gcttcaatca ctcccggcca atcggctgtg ttttacaatc aggatgtggt cctgagcggg
     2821 ggaattatca aggaagtcaa aacttaggcc ctggatgatc ctgttctgca aaaattcagc
     2881 agcaataata tattgacaac cgcagcacaa tttatagaat tatacttaaa gtcatcgaaa
     2941 taaggaaaat aaggtcaaag gaggactaat gcttacgatt acatatc
//
LOCUS       DSEB01000478            2839 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004653, whole genome shotgun sequence.
ACCESSION   DSEB01000478 DSEB01000000
VERSION     DSEB01000478.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004653"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            3..392
                     /locus_tag="ENO07_08530"
     CDS             3..392
                     /locus_tag="ENO07_08530"
                     /inference="COORDINATES: protein motif:HMM:PF14559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDS02050.1"
                     /translation="MIEIDSACSLAYRNLAVCETRLNNFENAYRVLTDYLELAGEDYN
                     LIVMAAEIAANLQKFDEAIKYGERALEINPHSPQLLTLLGDCYYSGGFLVSAKMSFEQ
                     ALKIDPNFDPARQRILQINEIQDARKR"
     gene            728..1189
                     /locus_tag="ENO07_08535"
     CDS             728..1189
                     /locus_tag="ENO07_08535"
                     /inference="COORDINATES: protein motif:HMM:PF02561.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HDS02051.1"
                     /translation="MKEKVGISISQALSSCQKNQILGMNQKDWIVLLFNAAADLLRQA
                     QKFLSERKPAEFSDSLERIRRIIYHLYDSLDIDQGGENAEHLGALYTYIIGQLYIVNS
                     TKNDKIISDLLVIINNLKEGWEGLNPQELSKGTFKHGAMPAGKRTVVSTKV"
     gene            1192..1641
                     /locus_tag="ENO07_08540"
     CDS             1192..1641
                     /locus_tag="ENO07_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS02052.1"
                     /translation="MEYSQVVELEEKLLELLRQEYSFYQSLYLLFDKQRDNLRFERDQ
                     KLIDLYNEIEKAERRIAESEDKIARLRSENRKVFNLAATSPEVKKLVTSIATLLKKNL
                     ALIKENEDFAKNKRNRIEEELEQVRRMYDHMACNKADNGARVFDGRS"
     gene            1725..1994
                     /locus_tag="ENO07_08545"
     CDS             1725..1994
                     /locus_tag="ENO07_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS02053.1"
                     /translation="MEINDNPEMKTQWNARTANRGKRMSEKENSPACGDEVETNTGVS
                     ERANFRQEVRADVVERARANIESGHYDEPEVVDEIVDRLMDQFGL"
     gene            2378..2454
                     /locus_tag="ENO07_08550"
     tRNA            2378..2454
                     /locus_tag="ENO07_08550"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:1.23"
                     /anticodon=(pos:2412..2414,aa:Val,seq:gac)
     gene            2496..>2839
                     /locus_tag="ENO07_08555"
     CDS             2496..>2839
                     /locus_tag="ENO07_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HDS02054.1"
                     /translation="MSSVKITFPDGSVSEYKAGITPMEIAENISKGLAKKVLAARVNG
                     SIINLNDPIKEDTELKLLTFEDREGREVFWHSSSHIMAQAVTDLFPNAKLAIGPPIDE
                     GWYYDFDVEKPFS"
BASE COUNT          842 a          606 c          729 g          662 t
ORIGIN      
        1 aggtgatcga aattgacagc gcctgcagcc tggcctaccg gaatctggca gtctgcgaaa
       61 cgaggctgaa caactttgaa aatgcctatc gggttttgac agattatctg gaacttgccg
      121 gtgaagatta taacctgatc gtcatggcgg ctgaaattgc tgcaaatctg cagaagttcg
      181 atgaggccat aaaatacggc gagagggctc tggagatcaa tccgcattcg ccgcaactcc
      241 tgaccctcct gggtgactgc tattactccg gcggcttcct ggtatcagct aaaatgagct
      301 tcgagcaggc cttaaagatc gatcccaact tcgatccggc ccggcagcgg attttgcaga
      361 taaatgaaat tcaggatgcc cgtaaacggt aggtttcagt ctgatcagcc atcgaaacat
      421 ccatagcggg accggctgtt gaggtaacct cgctgccccg catagactaa aatgcttttt
      481 ttgaaagccg gttttaaagt tgaaagacgg ggcgtttcaa tctgaatgaa ataaagaaaa
      541 gaagaaatat gccgatttat aacttgagaa gcataaaatt aaggaaaatg ttcgggtccc
      601 cgggaagatt taagaggggc ggataatcca tgggatatcc cgttgcggcc gctgaaatca
      661 accgagggat tttttgtacg ccatagatat ggattacata ttgtggataa cgataactac
      721 ttgagccatg aaagaaaaag tcggcatatc aatcagtcaa gcgctttcaa gttgccagaa
      781 gaaccagatc ttgggtatga accagaaaga ctggattgtg ctcctgttta atgctgcggc
      841 ggatctcctg agacaggccc agaaatttct gagtgagaga aagccggccg aattctccga
      901 ttcccttgag agaatccgcc ggataatcta tcatctatat gacagccttg atattgacca
      961 gggcggagaa aatgccgagc acctgggagc attatataca tatatcattg gtcagctata
     1021 cattgtcaat tcaaccaaaa acgataaaat aatatcagat ctgctggtga taataaataa
     1081 tctcaaagag ggctgggaag gcttaaatcc gcaggaatta tcaaagggga cattcaaaca
     1141 cggggcgatg ccggcaggaa agcgaaccgt tgtatcaaca aaggtgtgat tatggagtat
     1201 agtcaggttg tcgaattgga ggaaaaactg ctggagcttc ttcggcagga atattccttt
     1261 tatcagtctc tctacctgct cttcgataag cagagggaca atctcaggtt tgaacgggat
     1321 cagaaattga tcgatctgta caatgaaatc gaaaaagctg agaggagaat tgccgaatca
     1381 gaggacaaga ttgccaggct gcgcagtgaa aaccgcaagg tcttcaacct cgcggcaacc
     1441 tcgccagagg tcaaaaagct ggtgacatcc attgccaccc tgctcaagaa gaatctggcg
     1501 ctaattaagg aaaacgagga ctttgcaaaa aacaaacgca accggataga agaagaattg
     1561 gagcaggtca ggcgcatgta tgatcatatg gcctgtaata aggccgataa tggagccagg
     1621 gtctttgatg gcaggagctg agataacagg ttatttttat tatatatcat gatgggaaga
     1681 tgaagatcgc ataggaaacc atataacatg gttggaggcg agggatggaa atcaatgata
     1741 atcccgagat gaaaactcag tggaatgcca ggacggcaaa ccggggaaaa agaatgtcag
     1801 agaaggaaaa tagtcccgcg tgcggagacg aggttgaaac aaatacggga gtctccgaaa
     1861 gagcgaattt caggcaggaa gtccgagctg atgtagttga gcgggcgcgg gccaatattg
     1921 aatccggtca ttatgacgaa ccggaagttg tggacgaaat cgtcgatcgg ctgatggacc
     1981 aattcgggct ttgagaagac gactttttcg aatggtaatt agatatcccc gggcaaggat
     2041 tcttatttca tcggctggaa gatgaccgcc ccggttattt cacacccgga accgcgctcc
     2101 ggcggcaaaa aatccccgaa tccggtaagg ccggacaacg ggccagacct tgagatttcc
     2161 cgtaaatcgc cccggctcct gtgatatccg cgaagcctgt caggcacccg gctctcgtta
     2221 tattttctgc aatttttccg atagaaatct atatatatga caatatcttg cgtaaaacat
     2281 gcaggaagat ctaatttaat tcaaaaggac ttaatagctc cctttttgct tgacaaaatg
     2341 cttgtccagt ttaaattaac aggcctagtg aatagaaggg cgattagctc agttggttag
     2401 agtgcttgct tgacatgcaa gaggtcactg gttcgattcc agtatcgccc accatttaaa
     2461 cccaattgcg ggttttttta attgcagggt gtcgaatgag cagcgttaaa ataacatttc
     2521 ctgacggctc cgtaagcgaa tacaaagccg ggataacccc catggagata gccgagaaca
     2581 tttccaaggg gctggccaaa aaagtgctcg ctgccagggt gaacgggtcc atcataaacc
     2641 tgaacgatcc tataaaagag gataccgagt taaagctatt gaccttcgag gatcgcgagg
     2701 gacgggaggt cttctggcat tccagttccc atatcatggc ccaggcggtg acagatctct
     2761 ttccgaatgc caagctcgcc atcgggcccc ccatcgacga aggctggtat tacgattttg
     2821 atgtcgagaa gcccttttc
//
LOCUS       DSEB01000479            2705 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1005172, whole genome shotgun sequence.
ACCESSION   DSEB01000479 DSEB01000000
VERSION     DSEB01000479.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1005172"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1567)
                     /locus_tag="ENO07_08560"
     CDS             complement(<1..1567)
                     /locus_tag="ENO07_08560"
                     /inference="COORDINATES: protein
                     motif:HMM:PF00512.23,HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HDS02055.1"
                     /translation="MKTVIIGGGKACREIIELASGTFMKELRLEIVSVMDSDPDAPGM
                     VFARKKGIDTTTDIQQVMDTPDLELVLELTGRDHVLKQIHKIIPLGVKLIDHTIARIF
                     WDLLNAQTDQMARIDELATLEEKLDRERNFLQLLVDSIPELIVVLDKDKKVTRVNRSF
                     SSYIGITPDEAAGKTCIELFAGTDFEEHCKREVEMLDFTLRIGKSFSSIWMIPPPNEV
                     HWEVTRTPLISRGGSPRGILATWHRITDQVMLRREIETAEERFRSFIDSAHDWISMKD
                     LEGRYIIVNPVTAAAFGHKPEDFVGKRADEILPEKLARLIQKHDKEVIECNCHRSYEE
                     VIPIEGRDHHFQTVRFPLKDYKGSVQGVCTIMRDVTSEKELRDQLVQSSKLVAIGQLA
                     AGVAHEINNPLTGILAYAEDLADDLSSHDEHFEDIEVIIRETKRCRDIVRNLLDFARQ
                     EAPKLEKTSPNQIVTHALSLVEKLPQFRNIEIKQDIARDIPPVQCDQRQIQQVVLNLM
                     LNAADAMNSKGLIR"
     gene            complement(1646..2608)
                     /locus_tag="ENO07_08565"
     CDS             complement(1646..2608)
                     /locus_tag="ENO07_08565"
                     /inference="COORDINATES: protein motif:HMM:PF00069.23"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS02056.1"
                     /translation="MKETEKLTARIVELTGWKLYEPPRIITDTSNWMNIIRGDILRLG
                     GRDFVVKGNKHETRFGIGEQPKYWVFSVLDMESGEQKIIKTEFNEEFMAHIGIFKVRC
                     YRSPEKEGKVLEIVRGDRRFMQGYSVFDEKGNNIRVLDYIRGETFFNYIFSISKSHEE
                     YFHEDLGGLLWKLTESIEAIEFLHKHKFCHGDIRNDHIIIEADTGQYRWIDFDLNQMV
                     SDFDMWSIGNVLSYCVGKGINSFQQILRSKDFSWEVKESLRPTDASAFYEYRIMNLKK
                     LYPYVPERLNDILLHFTIKPKDYYVSIGQLLEDYRRMLERDFPI"
BASE COUNT          647 a          737 c          586 g          735 t
ORIGIN      
        1 gtctaataag tcccttgctg ttcatcgcat cggcagcgtt aagcatcaaa ttcaggacca
       61 cctgctgtat ctgcctctgg tcgcattgaa ccggaggaat atctctggca atgtcctgct
      121 tgatttctat attcctgaat tgaggcagct tttcaaccag agatagggca tgagtcacta
      181 tctggttcgg actggttttc tcaagcttgg gggcctcctg ccgtgcaaag tccaggagat
      241 tcctgacaat atcgcggcag cgcttggtct cacggataat tacctcaata tcctcaaaat
      301 gttcgtcatg actgctcaga tcatcggcca gatcctcggc gtaagccagg atgccggtca
      361 ggggattgtt tatctcatgg gccacgcccg cggccagctg ccctatcgcc accagcttgc
      421 tggactgcac cagctggtcc cgcagttcct tctcagaggt aacatcgcgc ataatagtgc
      481 agaccccctg tacgctgccc ttataatcct tgagcggaaa cctgacggtc tgaaagtggt
      541 ggtcacgccc ctcgatcggg attacctcct cataggagcg gtggcagttg cattcaatta
      601 cttccttgtc atgtttctgg atcagcctgg ccaacttctc cggcaatatt tcatcggccc
      661 ttttgccgac gaaatcctcc ggcttgtgcc caaaggctgc cgcagttacc ggattgacaa
      721 tgatgtaacg tccctccaga tccttcatgc ttatccagtc gtgggcggaa tcgatgaacg
      781 agcgaaatct ctcctccgcg gtctcgatct cccgccgcag catgacctga tcggtgatac
      841 gatgccatgt ggccaggatt ccgcgcggac ttcccccccg gcttatcagc ggggtacggg
      901 taacctccca atggacttca ttgggtggag gtatcatcca gatactggaa aatgatttgc
      961 caatcctcag ggtaaaatcc agcatctcaa cctcgcgctt gcaatgctct tcaaaatcag
     1021 tacccgcgaa cagctcgatg caggtctttc ccgcagcctc gtccggggta ataccgatgt
     1081 aactgctgaa actcctgttg acccgcgtca ctttcttatc cttatcgaga acaactatca
     1141 gttccggaat actatccacc agaagctgga ggaaattacg ttcccggtcc agcttctcct
     1201 ccagagtggc cagttcgtcg atacgcgcca tctgatctgt ctgggcattc aggagatccc
     1261 agaagattct cgcaatcgta tggtcgatca gcttgacacc caggggaata atcttgtgaa
     1321 tctgtttcag gacatgatcg cggcccgtaa gctcgagaac cagttccaga tcgggagtat
     1381 ccataacctg ctggatatcg gtggttgtat cgattccctt tttgcgagca aaaaccatac
     1441 cgggagcgtc aggatcagaa tccatcacac ttacgatctc caggcgcaat tctttcatga
     1501 atgtcccgga agcaagctcg attatttcac ggcaggcttt gcccccgcca atgatcacgg
     1561 tcttcatatt tttccctcag aaccggttta tccaggctat ctcacccata aacataatcg
     1621 atatataaaa aataacaaaa ttttatcaga ttggaaagtc cctttccagc atcctgcggt
     1681 aatcctccaa tagctggcca atgctgacat aatagtcttt cggctttatt gtgaaatgca
     1741 gaagaatatc gttcagcctt tcgggaacat acgggtatag ctttttgaga ttcatgatcc
     1801 ggtactcata aaaagcggag gcgtcggtcg gacgcaggct ttcctttacc tcccacgaaa
     1861 aatccttgct gcgcaaaatc tgctgaaacg agtttatccc tttgcccacg caataactca
     1921 agacattacc gatactccac atatcgaaat ccgagaccat ctggttcagg tcaaaatcga
     1981 tccacctgta ttgcccggta tcggcttcaa ttattatgtg gtcgttcctg atatcgccgt
     2041 ggcagaactt gtgtttatgc aagaattcta tggcttcgat ggattcggtc aatttccaga
     2101 gcagccctcc caggtcctca tggaaatatt cctcatggct cttggatata ctgaaaatat
     2161 aattaaaaaa tgtctcgccc ctgatataat cgaggaccct gatattattg cctttttcat
     2221 caaagacgct gtaaccctgc atgaaacgcc tgtcgcccct gactatttcc agaaccttgc
     2281 cctccttttc gggggagcgg taacagcgca ccttgaaaat tccgatgtgc gccataaact
     2341 cttcattaaa ttcggtcttg atgatcttct gctcgccgga ttccatatcg agcacagaaa
     2401 agacccagta tttgggctgc tcgccgatac cgaaacgggt ttcatgttta ttgcccttga
     2461 ccacaaaatc ccgcccgcca agcctcagga tatcacccct gataatattc atccagttcg
     2521 acgtatcggt tataatccgc ggaggttcat atagtttcca tcctgtcagt tcgacgatcc
     2581 gggcggtcaa tttttcggtt tctttcattt cagttcagtt catttagaag ttattttctc
     2641 ttgcactatt aatatcgcca atctgcagat gatttcaaca attatcctca agaagacctt
     2701 ataac
//
LOCUS       DSEB01000480            2817 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004733, whole genome shotgun sequence.
ACCESSION   DSEB01000480 DSEB01000000
VERSION     DSEB01000480.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004733"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(227..1840)
                     /locus_tag="ENO07_08570"
     CDS             complement(227..1840)
                     /locus_tag="ENO07_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HDS02057.1"
                     /translation="MSAIVFQTNKKTGVTYAYESISYWDKEKQQSRAKRKCIGKLDPD
                     TKKIIPNKPGKKPAGHHIKKAKPGPTAITRTARNFYGATYLFDRIGEETGVTENLKAC
                     FPDSYRQILSVAYYLIMEDKNPLSRFPRWAAMHQHPHGEIISSQRSSELFASITEESK
                     QNFFRLQGKRRVEREYLAYDSTSVSSYSECLRQVRYGKNKDHEHLAQINLSLLFGQQS
                     QLPFYYRKLSGNIPDVKTLRKLLADMKTLGYEKIKVVLDRGFFSAANINDLYKYHMKF
                     LIAAKVSLKLVQKHLETVRDTMRSWTWYNQNYQLYAISLPMTWPYTQDRPYKRETIKA
                     DRRLYLHLYYSPERALEDEKSFNNRLSVLQEELESGQRHPDHEAQYARYFEVKTTPVR
                     GITVTAKEEALREAKRNYGYFTLLSNDIKEPVTAIEIYRKKDMVEKAFNNLKERLSLR
                     RVAVSSEQSLDGKLFVQYIALIFLSCITKRMQEKKLFKNYTLQEVLDELDIIECFKSP
                     GKQLQVGEITQRQRDLYVKLGISSPSSLQ"
     gene            1966..2361
                     /locus_tag="ENO07_08575"
     CDS             1966..2361
                     /locus_tag="ENO07_08575"
                     /inference="COORDINATES: protein motif:HMM:PF00474.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS02058.1"
                     /translation="MQDIYEKILHKGKPLKEAVKLRWSRWVLLFSGILALLMAYLAQD
                     LIFWLVLFAWAGLGASFGPVLILSLYWKKMTRAGVISGMVTGTVVTLIWKIFLKDATG
                     IYELIPAFLFSVLAAVLVSLLSGSGSRRA"
BASE COUNT          649 a          658 c          709 g          801 t
ORIGIN      
        1 acagagccct gccggaagat tcttcaagcg atgaattcag gcgaatcttg tcgagccgga
       61 ggctcaatcg cgccggattc cattttacat gacctatttc caaaaccagg ccgtactgtg
      121 tcttcagggt tttcccgatc tgccgaaccg cccatttctt aaagggcggg ctgatgagaa
      181 ccagataaag aattacaatg actgatatta aacctgaatt cccgatttat tgtaacgagg
      241 aaggcgatga gatgcccaac ttcacatata ggtccctctg gcgttgggtt atctcgccga
      301 cctgcaattg tttaccggga gacttaaaac actcaatgat atcgagctca tccaacactt
      361 cctgcagggt gtaattcttg aacagctttt tttcctgcat tcgcttcgtg atacaggaca
      421 agaagatcag ggcgatatat tgaacaaaga gcttgccgtc gagactctgt tctgatgaaa
      481 cggcgactcg gcgcagactc agtctttcct tcaaattatt gaaggccttt tccaccatat
      541 cttttttcct gtagatctca attgctgtga cgggttcctt gatgtcattg ctcaggaggg
      601 tgaaataacc ataatttctt tttgcctctc tcaaagcctc ttctttggcc gtgaccgtta
      661 tgcctcggac gggcgtcgtt ttcacttcaa agtatctggc atattgggcc tcatgatccg
      721 gatgacgctg tccgctttcc aattcctcct gcaggacgga taagcggttg ttgaaggatt
      781 tctcatcctc cagggcccgt tccggagaat aataaagatg aagatacagc cggcgatccg
      841 ctttgattgt ctctctttta taaggacgat cttgagtata gggccaggtc atcggcaggg
      901 aaattgcata gagttgatag ttctggttgt accatgtcca gctgcgcatc gtgtcccgaa
      961 cggtttccag gtgtttctgt acaagtttca gcgatacctt ggcggcaatc aggaatttca
     1021 tatgatactt gtaaagatca ttaatgttgg ccgcgctgaa aaacccccgg tccagaacca
     1081 ctttgatctt ttcatagccc agcgttttca tgtccgccag gagcttcctc agggttttca
     1141 cgtcgggaat gttgcctgac agcttccggt aataaaacgg caattgggat tgttggccga
     1201 aaaggagtga cagattgatc tgagccaggt gctcatgatc cttattcttg ccgtaacgca
     1261 cctgtctgag acattcagaa taactggaaa ccgaggtgct atcataagcc aggtactccc
     1321 gctccacacg gcgtttccct tgaagtcgaa agaagttctg tttggactct tcggtgatag
     1381 aggcgaaaag ttcgctgctt ctctgggagg agatgatctc accgtgggga tgctgatgca
     1441 tggctgccca tcggggaaaa cggctcaagg gatttttgtc ctccataatc aggtagtagg
     1501 caaccgacag aatctggcga taagagtccg ggaaacaggc ttttaaattc tcagtcactc
     1561 cggtctcctc tccgattcga tcaaagaggt aggtggcccc ataaaaattg cgagccgtac
     1621 gggttatcgc cgtagggcct ggcttggctt ttttgatatg atgaccggca ggttttttcc
     1681 ccggtttgtt gggaatgatt tttttggtgt cgggatccag cttcccaatg catttgcgtt
     1741 tggcgcgaga ctgctgtttt tccttatccc agtaagagat ggactcgtat gcataagtga
     1801 cgcccgtctt tttattggtc tgaaaaacga tggctgacat gtacgacttc tcctgtcctc
     1861 gttacaataa tatagtatgt aacgagatct aatgcaagat aaatcgtatt tatgtcatta
     1921 ttttccagag tgttatcgtg atctcatcgt tacaatcatc cgggaatgca ggatatctat
     1981 gagaaaattc tccataaagg aaagccgctg aaagaggctg tgaaacttcg ctggagccgc
     2041 tgggtgttgc tgttttcagg aatcctggcc ctgctgatgg cctatctggc tcaagatctg
     2101 atcttctggc tggttctttt tgcctgggct ggactcggtg cctctttcgg tccggtcctg
     2161 attctttccc tttattggaa gaaaatgact cgggcgggag tgatttcagg aatggttacc
     2221 ggaacagttg taacgctgat atggaaaata tttctcaagg acgctaccgg aatctatgaa
     2281 ttgataccgg cttttttgtt ttccgttctg gcagcggtac tggtcagcct tttatccggc
     2341 tccgggtctc gccgagcttg atcagggcgg tttcggtatc cggagcgcca taaacgaaaa
     2401 taagatattt atcttcagga gaaaccacga tcaggagttc atccttgtcc tttgcggcgc
     2461 aggcggcagg atctcccgcc ataacagcaa tgaagttctg atcgatggag aactgctcat
     2521 tgacatgggt taaggcttcc tgggatgcct gggcatcggc ataggagaag atgaaaacag
     2581 ccagctccga gtcatagttt ccccggacgc cttcattgaa cgacagggtg ttttcattga
     2641 agaagcggtg gctgttgaaa agcccgagat atcccttgaa atattcccgg cttccgtcga
     2701 taagtccttc ttcgggaagg agaccggcga tttccggcag cgaaccggtg tctttcaggc
     2761 gctcgtcgag cgccgcagcg atgatttcca ggcctttgat ggtgctttct tcctcat
//
LOCUS       DSEB01000481            2816 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1004738, whole genome shotgun sequence.
ACCESSION   DSEB01000481 DSEB01000000
VERSION     DSEB01000481.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1004738"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(100..849)
                     /locus_tag="ENO07_08580"
     CDS             complement(100..849)
                     /locus_tag="ENO07_08580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDS02059.1"
                     /translation="MQESFTYFYIPPSLVTGEEIAFQEDESHHIAKVCRQQVGDEIYA
                     VDGIGNRYLVEITMCTPQRVEGKILETDMDVNEPHTKLSLALPWTKDPKLDLIITKAT
                     ELGVSKFVYYTADKSIIKKDDDRIEKKIERHRRLGISAIKQSLRCVLPEMKDIGRSHN
                     FDKCLKYYDICLYADLNKDALPLQTALKGHLPKWILLVIGPEAGFSKEEKITLENSGA
                     LPISLGPRRLRFETAALAFTSMALNILGEME"
     gene            complement(851..1744)
                     /gene="prmA"
                     /locus_tag="ENO07_08585"
     CDS             complement(851..1744)
                     /gene="prmA"
                     /locus_tag="ENO07_08585"
                     /inference="COORDINATES: protein motif:HMM:PF06325.11"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDS02060.1"
                     /translation="MSDRPGHKSGRGYLEIAVTIPCQHEEMVSDFIIEEVAFGLVTED
                     LDDQVRIICYIPHDNGPDEKIDKLKKYLSIMEVLPDELIDERIKTKNIEEIDWINEYQ
                     KSFEPVVIGDLVIKTPWDKTEFPGKKIIIIEPKMAFGTGKHETTQLCLRAIEKEIEPG
                     MKVLDLGTGSGILSIYAAMLGASEILGLDIDPDAVPNALENAEMNNVSDKFGARTGSM
                     EQIDKKNYYDLVISNLIYDGIVKLFDDFLAALKPGGKMILSGILDDQKNQLTEFIKFK
                     ASFEIEIMQQNEWLCYILRTR"
     gene            complement(1737..>2816)
                     /locus_tag="ENO07_08590"
     CDS             complement(1737..>2816)
                     /locus_tag="ENO07_08590"
                     /inference="COORDINATES: protein motif:HMM:PRK10767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HDS02061.1"
                     /translation="LQYHPDRNPGDKSAEDNFKEATEAYEVLKNPQTRQVYDQYGHAG
                     LRQGAGAGGFGGGFAGFDIADALRAFMRDFGGFGGFEDIFGGFGGTSNRGRTVHRGRD
                     LRVDLQLSLEEIAEGVSKKIKVNYKSTCDSCNGSGAANEEAKKTCPQCKGSGEIRQIT
                     RSLLGQMVRVAPCDYCKGDGTIITDFCPECHGSGLVDDKKTITVRIPPGVTTGNYLTM
                     SGEGNHGPRGGPPGDVIVVVTEKEHEHFTRSGDDIVYEVPISFSQAALGTKLNVPTLA
                     GESELNVPAGTQSGKVFKFRNKGIKHLRGLGRGDQLVRVIVWTPTKLGPEEKKLFEQL
                     ADNEKINPPRSSKSFFEKLKETLGV"
BASE COUNT          656 a          738 c          628 g          794 t
ORIGIN      
        1 ataaagccca ggaaaatata caccggaagt gtctgtgtcc attcatatgc cataatagta
       61 ttttcggaag agggcgagat taattaacag gccccgttgc tattccatct cccccaagat
      121 attcagggcc atcgaggtaa aagcaagggc agcggtttcg aagcgcaagc gcctgggacc
      181 caaagagata ggcagtgcgc ctgaattttc gagcgttatc ttctcctctt tgctgaatcc
      241 ggcttcgggc ccgatgacaa gtagtatcca ctttggaaga tggcccttta acgcggtttg
      301 aagcggtaac gcatccttat tgaggtccgc ataaaggcat atatcataat attttaagca
      361 tttatcgaaa ttgtgtgagc ggcctatatc cttcatctcc ggcagaacgc atcgtaaaga
      421 ctgttttatg gcgctgatcc ccagcctgcg atgcctttca atcttcttct cgatccggtc
      481 atcatccttc ttgataatcg acttatccgc agtataatag acgaattttg atacgcccaa
      541 ttcggtcgct ttggtgataa tcaggtccag cttgggatct tttgtccagg gcagggccag
      601 agataatttg gtatgcggct cattcacatc catatctgtt tcgaggatct tgccctccac
      661 gcgctgaggc gtgcacatgg ttatttccac cagatagcgg ttgccgatgc catcaacagc
      721 ataaatttca tcacccacct gctggcggca gactttggct atatgatggc tctcatcttc
      781 ctgaaaggct atctcttctc cggttaccag agagggcgga atatagaaat aagtaaatga
      841 ttcctgcacg ttatcgagtc ctcaggatgt aacacagcca ttcattctgc tgcattatct
      901 ctatctcaaa agatgcttta aacttaatga attcggtcag ttgatttttc tgatcatcaa
      961 gaataccgga gagaatcatt ttaccgcccg gtttaagcgc cgccaaaaaa tcgtcaaata
     1021 gtttaacaat tccgtcatat atcaggttcg aaattaccag atcataataa ttttttttat
     1081 caatctgttc catagaaccg gtcctggccc caaatttatc cgagacattg ttcatttccg
     1141 cattctccaa agcattaggc actgcgtctg gatcgatatc cagtcccaga atttcggacg
     1201 ctcccagcat ggccgcataa attgacaaaa tacctgatcc ggtacccaga tcaaggactt
     1261 tcattcccgg ctctatctct ttttcgattg cccgcaggca taattgagtg gtttcatgct
     1321 taccggtgcc gaaagccatc ttgggttcga ttataattat tttttttccg ggaaattcag
     1381 ttttgtccca gggcgtcttt attaccaggt cgccgatcac aaccggctca aagctctttt
     1441 gatattcgtt gatccagtcg atttcctcaa tatttttcgt cttgatgcgc tcgtcgatca
     1501 gctcgtcagg aaggacctcc atgatggaga gatatttttt taatttgtct atcttttcgt
     1561 cagggccgtt gtcatgggga atataacaga taatcctgac ctgatcatcg aggtcttcag
     1621 ttacaagccc gaaagcgacc tcttcgatta tgaaatcgga aaccatctcc tcatgctggc
     1681 aaggtatggt gacggcaatt tccaggtatc cccgcccgga tttatgtccg ggcctgtcag
     1741 acacccagag tctcctttaa tttctcgaag aaactttttg atgacctggg aggattgatt
     1801 ttttcattat cggccagttg ttcaaacaac tttttttctt cggggccgag tttcgtgggc
     1861 gtccacacaa ttacacgtac gagttgatcc cctcttccga ggccgcgcag atgcttgatg
     1921 cctttgttcc tgaacttgaa aaccttgccg gattgagttc cggctggtac gttcagttca
     1981 ctctcaccgg cgagggtggg cacatttaat ttggttccga gcgccgcctg agaaaatgag
     2041 atcgggacct cgtagactat atcgtccccg ctgcgcgtga aatgctcgtg ttccttttca
     2101 gttacgacaa caataacatc accgggaggg ccgccacgcg ggccgtgatt accttccccc
     2161 gacatggtca ggtagtttcc ggtagtgacc ccgggaggaa ttctgacggt aattgtcttt
     2221 ttgtcatcta caagtccgct gccatggcat tccgggcaga agtcggtaat gatcgtaccg
     2281 tcccccttgc agtagtcgca gggagcgaca cggaccatct gccccaacaa tgaccgggtt
     2341 atttgacgaa tctcgcctga gcccttgcac tgaggacagg tctttttggc ctcttcattt
     2401 gccgctccgc ttccattgca gctatcacag gtggatttgt agtttacttt gatcttcttg
     2461 ctgactcctt cggcaatctc ctccagcgat aactggagat cgacccgcaa atcccggccg
     2521 cgatggaccg ttcggccgcg atttgacgtc ccgccgaaac cgccgaagat atcctcgaat
     2581 ccgccgaatc ccccaaaatc ccgcatgaag gctcgcagag cgtctgctat atcaaatcca
     2641 gcgaagccac cgccgaatcc gccagctcca gccccctgcc tgagcccggc atgaccatac
     2701 tgatcatata cctggcgggt ctgaggattt ttcaaaacct cataagcttc tgtggcctcc
     2761 ttaaaattat cctcggcact tttgtcgccc ggatttcgat ccggatgata ctgtag
//
LOCUS       DSEB01000482            3199 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003596, whole genome shotgun sequence.
ACCESSION   DSEB01000482 DSEB01000000
VERSION     DSEB01000482.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003596"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(96..428)
                     /locus_tag="ENO07_08595"
     CDS             complement(96..428)
                     /locus_tag="ENO07_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDS02062.1"
                     /translation="MNIYVGNLSHDTTEDELRRAFEEYGTVDKVNIIMDKFSGRSKGF
                     GFVEMASKEEAEAAMNALNNKELDGRTLNVNEARPRTDRGSRGGGGGGRGRGGHGGGG
                     YGGNRRSW"
     gene            complement(674..2566)
                     /locus_tag="ENO07_08600"
     CDS             complement(674..2566)
                     /locus_tag="ENO07_08600"
                     /inference="COORDINATES: protein motif:HMM:PF01841.17"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HDS02063.1"
                     /translation="MKTSKHLLCRIPSTLLALMLIASGAMADSDDAAVIHDKQVTCKI
                     KGNKEIETEHYQKIEVLNRDGAFYALKIIEESSLLELKEFSGKLYDSEGNPVEEIERD
                     DGKTICGYGSYEVYSDYCYTIFGFSRDSYPFTVEFSYKLKQKTLFQWQDWRPQSYIPV
                     KHAKYTLITDDDFVFQYKSSEHIPPPEIIESGGDKIYTWEMNDVPELEEGDYASIIEF
                     GGIAIQFAPPEYKLGKYKFDGSSWNTLSHGNYKMIQGCLDISDEQKDLMDRIAAESEN
                     EFEICRKLHRKMSELARYVAIHVDIGGWLPHKSRDTFERGYGDCKDLSTLYVSMLRYA
                     GIDAKYVLLSTRTAGITDPDFPSLARFNHAILFAVADGDTIWIDPTRNDCAIGDLPWA
                     DENTYALVVDEEMGRLVRTDSSEAEENVISRKVELCPQKNRSVAVSMEYRLTGNPRDH
                     FNAFISSYSETSRANILRNHMYKLSDKFLLDSLNFPKGGIDQPVQKLSLWGQVRSALH
                     RIGGEQIMDLTFLDYESKKKTYIDPETYKQPFEFLFPKKFVDTVIIHLPDSKAAVGLP
                     DTADIKDDIGAYRICCQADDSLVVITRETSNYQYRVMPEGFFDYWAHRDKVKGLGECF
                     IKLGNR"
     gene            3043..>3199
                     /gene="rpsU"
                     /locus_tag="ENO07_08605"
     CDS             3043..>3199
                     /gene="rpsU"
                     /locus_tag="ENO07_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDS02064.1"
                     /translation="MVGVRVRDDESFEKALRRFNKFCEKNGILSDIKKHQHFEKPSDR
                     RKRKINAA"
BASE COUNT          788 a          786 c          714 g          911 t
ORIGIN      
        1 atatttgtcc tgaggaatcc ctgtactcaa aagttcggcc gaaaaaaaag gaccgaatag
       61 aattcggtcc tgttagtttc attgtcttaa gcggattacc aggaacgtct gttcccgccg
      121 taacctccgc cgccgtggcc gccgcgtccg cgtccgccgc cgccgccgcc tctggaaccg
      181 cggtcggtgc ggggacgagc ctcgttgaca ttcaatgtcc tgccatcaag ctctttgttg
      241 ttgagcgcgt tcatggcagc ctcggcctct tctttggaag ccatttcaac aaacccaaag
      301 ccctttgagc gaccactgaa cttgtccatg ataatgttga ctttgtcgac tgtgccgtac
      361 tcttcaaatg cacgccgaag ttcatcctct gtcgtatcgt gcgacagatt tcctacgtag
      421 atattcattc tactctccaa attatgttta ccagatccgg attcgaaaat ctaaaaccct
      481 gctatttgat aaacattcct ttaataagtt gctgttaaat tgtcaccaga ttaagttcat
      541 tcaaagctca gctttgggga tgcaaatcct gggaaaaaat aatgtgggac aaccattgtc
      601 atgagcccaa taagaataat tggattaaaa aagtcaatct ttattttgaa cttccgctat
      661 tttaaatttg atttcacctg ttccccagtt tgataaaaca ttcgcccagc cccttgacct
      721 tatcccggtg ggcccagtag tcgaagaagc cttccggcat gacgcggtat tggtagttgc
      781 ttgtctctct ggtaattacc accaggctgt catcggcctg acagcaaatc ctgtaagccc
      841 cgatatcatc ctttatatcg gccgtatccg gaagccccac tgccgctttt gaatcgggca
      901 gatggattat tacagtatcc acaaatttct tgggaaagag gaattcgaag ggctgtttgt
      961 atgtttccgg gtcgatgtag gtctttttct tgctctcata atccagaaat gtcaaatcca
     1021 tgatctgttc accgccgatc ctgtgaagtg cgctgcgtac ctggccccat aaactcagtt
     1081 tctgaacagg ctgatcgatc ccacctttgg gaaaatttag gctgtccaga agaaacttgt
     1141 ctgagagttt atacatgtgg tttctcaata tattggccct gcttgtctca gagtaagagg
     1201 aaatgaaagc attgaaatga tcccgcggat tgccggtcag ccgatattcc atgctgacag
     1261 ccaccgatcg atttttctgc gggcagagtt ccactttgcg gctgatcacg ttctcctcgg
     1321 cttctgatga atccgtacgc accagccggc ccatctcctc atccaccacc agggcatacg
     1381 tattttcatc ggcccatggc aggtccccga tagcgcagtc gttacgcgtg ggatctatcc
     1441 agatcgtatc gccgtcagcc acagcaaaga ggatggcatg attgaatcgt gccaaagatg
     1501 gaaagtcggg atcggttatt cccgcagtgc gggtggaaag caggacatat ttggcatcga
     1561 taccggcata ccggagcatg gagacataaa gcgtgctgag atccttgcaa tcgccgtaac
     1621 cgcgctcgaa agtatccctg gatttatgcg ggagccatcc cccgatgtcc acatggatgg
     1681 ccacatatcg ggccagctca gacatttttc tgtgcagctt gcggcagatt tcaaattcgt
     1741 tttcagattc ggcagcaatc cggtccatca agtctttctg ctcatcactg atatcgagac
     1801 atccctgaat catcttgtaa ttgccgtgag acagtgtgtt ccagctgctg ccgtcaaatt
     1861 tgtacttgcc gagcttgtat tccggcggtg caaactggat ggcgattccg ccgaactcga
     1921 ttatactggc atagtccccc tcttccagct caggcacatc attcatctcc caggtgtaaa
     1981 tcttatctcc gccggattct atgatttcgg gcggtggaat atgttcgctg cttttatact
     2041 ggaagacaaa gtcatcatcg gtgatcagag tgtattttgc atgcttgaca gggatatatg
     2101 actgcggcct ccagtcctgc cattgaaaga gagtcttctg tttgagttta tatgagaatt
     2161 cgacggtgaa gggataactg tctctcgaaa atccgaaaat agtataacag taatcggaat
     2221 aaacctcgta gctcccgtat ccgcagatag ttttgccgtc atccctctcg atttcctcta
     2281 ccgggtttcc ctccgaatca tataactttc cggaaaactc cttcaactcc aggagcgagg
     2341 attcctctat tattttgagg gcataaaagg cgccgtcacg attcagaact tcgattttct
     2401 ggtaatgttc tgtttctatt tctttattgc ccttaatctt gcaggtcacc tgcttatcgt
     2461 ggatcactgc ggcatcatcc gaatcggcca tggcgccaga ggcgataagc atcagcgcca
     2521 gcagtgtgct cgggatgcgg cacagcaggt gttttgatgt tttcataata gctccccgtt
     2581 cgtactgatt atctccaact ggaccggcgc tatcctgtca aatgactatt ttttcacgac
     2641 cttgaggttc aattccgaaa tcacgtggat agtctgcctc tcctgttttc ccccaagagc
     2701 gtcggtgttg ctgttcttcc cccaatcgta cggccccgcc agatgatcgt gcccatatag
     2761 tagtaaatta taagaccgga cacccataat ggcaataatt ttttttgagc gacttgacgt
     2821 tatgcccggc ctttaattga caggccatcg gtcaatttct aacttattcg gaaccaacgc
     2881 atttcgaaat tgttttagag tatgccgtga ttattcgata attcattcgt tttataaaag
     2941 aaatcttgac ataaatcatt aattatttaa tatttaggcg aaattttatg cgtaactgcc
     3001 gatatttcgg caagatagaa taccggaaag gagttgatat tattggtagg cgtacgtgta
     3061 cgcgatgatg aatcgtttga aaaagcactg agacgtttta ataagttctg tgagaaaaac
     3121 ggaattcttt cggacattaa aaagcatcag cattttgaaa aaccctccga tcggaggaag
     3181 agaaagataa atgcggcca
//
LOCUS       DSEB01000483            3596 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1002766, whole genome shotgun sequence.
ACCESSION   DSEB01000483 DSEB01000000
VERSION     DSEB01000483.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1002766"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..699)
                     /locus_tag="ENO07_08610"
     CDS             complement(<1..699)
                     /locus_tag="ENO07_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS02065.1"
                     /translation="MKPLILLAIIAASILTLSLGLFGQDLQQDFESYDQIRDSLDSLY
                     DLEKYAQAIDLIKSVYGLFPEDDFELTRSHTLFCRMAGLYEVCMDVFEAGHKKGYFYF
                     IMPGMKKYEPFTEFERFDDVVTEDARLREEANKESKTLMEVETPQGYSPDRQYPLLIV
                     LHGGGSSIDRAKKHWHSAKLRNEYIVAFLQSYMHMDLITYGWRTSDQRAQEDIKKIYD
                     ELINNYSIDTARVVV"
     gene            complement(829..2496)
                     /locus_tag="ENO07_08615"
     CDS             complement(829..2496)
                     /locus_tag="ENO07_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HDS02066.1"
                     /translation="MKTLTIFICICIIASFVYSQETRAESDSLKITKEDIVKAQELTG
                     LEFAEPEIDSMLDELNTNLGFYREMREIEIDNSVPPAILFNPIPSGIEFESGEEPLKL
                     SAPGNDRRPEDFDDLAFYSVRDLAELVRTRQVTSVELTEFALERLKKYDPLLHCVITL
                     TEELALEQAARADAEIAAGKYRGPLHGIPYGAKDLLAVKEYRTTWGAEPYKEQVINYD
                     AAVIRKLQDAGAVLVAKLSLGALAWGDVWFADTTRNPWNLEQGSSGSSAGSAAAVSAG
                     LVPFAIGTETWGSIVSPSTICGATGLRPSFGRVSRTGAMALSWSMDKIGPICRTVEDC
                     AIVFDAILGPDGHDKSVIDVPFNYNHQILSDQVRVGYLKRDFETDTMYYEHNMTALEM
                     MRALGFDLIAIDLPDIPVNPTQIILSAEAAAAFDELTRSGRDDLMVRQIKNAWPNVFR
                     ASRFIPAVEYIQANRLREMAIDSMAAIMQDIDVYISPTFEGDNLLLTNLTGHPCVVLP
                     NGFAEDGTPVSITFIGGLFDEATLLAVAKMYQDATGFHLKHPPLFKD"
     gene            2722..3459
                     /locus_tag="ENO07_08620"
     CDS             2722..3459
                     /locus_tag="ENO07_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS02067.1"
                     /translation="MISRIIRRGLFLPLVLIFGCCSEEGTSPDPFQKETCAIEAKYDY
                     IRSVKGGGGIFIIRLVPGDDLKDPVELCLQANDCMHARLDRTALDVHSSVAEITVNPD
                     SACQIKVYPIEIRASNSEFSQSIILEAEIMLWHQCYPEYALQVLNDFDEWLMKNHPEY
                     ADLSAQDWYCYDTYPQILVVSHWTLLSPEYEIRICHHNTIYPHNWTQFLLRKRGEFDA
                     MLAVKRDNDSTALHEIPPSKYPTLFGY"
BASE COUNT          970 a          901 c          779 g          946 t
ORIGIN      
        1 aactacgact cttgcagtgt cgatcgaata attgtttata agttcatcat agattttctt
       61 aatgtcttct tgtgcgcgtt gatccgaggt gcgccatcca tatgtgatca gatccatatg
      121 catataagat tgcaggaaag ctacaatgta ctcattacgc agctttgcag agtgccagtg
      181 ttttttggcc ctatcaatcg aacttccgcc tccatgcaag actatcagga gcgggtattg
      241 tctgtccgga gagtaacctt gcggagtttc aacttccatt aatgtctttg actctttatt
      301 ggcctcttct cgtaagcgcg catcctcagt gactacatca tcaaacctct cgaactccgt
      361 aaacggctca tactttttca tgccgggcat aatgaagtag aaatatcctt ttttatgccc
      421 tgcttcaaaa acatccatgc atacttcgta aagtccggcc atgcggcaga aaagagtatg
      481 tgatctggtt aattcaaaat catcttccgg aaacaggccg tacaccgact ttatcaggtc
      541 aatcgcctgc gcatatttct ccagatcata gagactatcg aggctgtcac ggatttgatc
      601 atagctctca aaatcctgct gaagatcctg accaaaaagg ccaaggctaa gtgtcagaat
      661 cgaggccgca ataatggcca gcaaaatcaa tggcttcatg cagatcctcc cgggatagca
      721 atatttacat tgcagttgat tttatttgaa tacggccatt tactcaattt gttcatttac
      781 ttttgtaatc catgcaacat cccgcggttt cccgattatc ctgccatctt aatccttaaa
      841 caagggtgga tgcttcaggt gaaagccggt ggcatcctga tacatctttg ccacagccag
      901 aagcgtggcc tcatcaaaca atccgccaat aaacgttatg ctgacaggtg tgccgtcttc
      961 ggcaaatcca ttgggaagaa ccacgcatgg atgcccggta aggttggtca aaagcaaatt
     1021 atccccttca aacgttggtg agatataaac atcaatatct tgcataatgg cggccatact
     1081 gtcgatcgcc atctcacgca gacgattagc ctgaatatat tccaccgcgg gaataaaacg
     1141 gctggcgcgg aaaacattgg gccaggcatt cttgatctgc ctgaccatca gatcatcccg
     1201 tcccgaccgg gttaactcat caaaggcagc ggccgcctcc gccgacagaa ttatctgcgt
     1261 cggattgacg ggaatatcag gcaaatcaat cgcaatcagg tcaaacccaa gcgctctcat
     1321 catctcgagg gcggtcatat tgtgttcata atacatcgta tccgtttcga aatctctctt
     1381 aagatatcca accctgacct gatccgacag gatttgatgg ttataattaa acggaacgtc
     1441 gatcacgctt ttatcgtgtc cgtccggacc cagaattgca tcgaatacta tggcgcaatc
     1501 ctcaacagtt cggcagatcg gcccgatctt atccatagac cagctcaagg ccatagctcc
     1561 ggtacggctg acccgtccga agctcgggcg aagcccggta gcgccgcata tggttgaggg
     1621 cgagacaatc gagccccagg tctcggtgcc aatcgcaaac ggcaccaggc cggccgagac
     1681 tgccgccgcc gaccccgccg aggatccgct tgaaccctgt tccaggttcc atggattacg
     1741 ggtagtgtca gcaaaccaga cgtctcccca ggccagcgcc cccagtgaca gcttggccac
     1801 cagaaccgcc ccggcatcct gcaatttccg aatcacagca gcatcataat ttataacctg
     1861 ttccttgtac ggttcggctc cccaggttgt cctatactct ttgactgcca gcaaatcctt
     1921 ggctccgtat ggaatcccat gcaacgggcc ccggtatttt cctgcagcaa tttcggcatc
     1981 cgcccgcgcc gcctgctcca atgccagctc ctcggtcagg gtgatcacgc aatgaagcag
     2041 gggatcgtat tttttcagcc gttccagagc aaattcagtc aattccaccg aggttacctg
     2101 ccgggtcctc acaagctccg ccaggtcgcg caccgaatag aatgcaagat catcaaaatc
     2161 ctccgggcgc ctgtcattgc cgggcgcaga taattttaat ggctcctcac ccgactcaaa
     2221 ttcaattcct gagggtatcg gattaaataa tatggccggc ggtacagaat tatcgatttc
     2281 aatctcgcgc atttctcgat aaaatcccag attcgtattg agctcatcca gcattgagtc
     2341 aatctccggc tctgcaaact caagtccggt tagctcctgt gccttaacaa tatcctcttt
     2401 agttatcttc aggctatccg attcggctct ggtttcttgc gaataaacaa acgaggcaat
     2461 tatgcaaatg cagataaata tggttagagt tttcatattt tctccctgat ttcaatttgt
     2521 tattcgatga tcattcaagt tgcgttctgc ccgtccaaat atcaagcttt ttcaataaaa
     2581 taaaaatcag aattaaacct ggggaagaat taataaacaa acgaatccat tatgcgtctg
     2641 aatagatgag agcctctatt tgctaacgat tttcaaacgc ctcaagcgac catttttaat
     2701 atgaattaca ggagtacggt tatgataagc agaataatca gaagaggttt gtttctgccg
     2761 ctggttctaa tttttggctg ttgctcggaa gaggggacct cccccgatcc attccaaaaa
     2821 gagacctgtg cgattgaagc aaagtatgat tatatcagga gcgttaaggg cggcggaggg
     2881 atatttataa ttcggctggt gcccggtgat gacttaaagg acccggttga actatgttta
     2941 caagcaaacg attgtatgca tgctcgcctg gatagaacag cattggatgt gcattcaagt
     3001 gtggccgaga taactgtaaa tcccgacagc gcctgtcaga taaaagtata cccgatagag
     3061 atacgagcgt caaacagcga attttctcaa tctatcattc ttgaggcgga aataatgcta
     3121 tggcaccagt gttatcctga atatgctttg caggtcctga atgatttcga tgagtggctt
     3181 atgaaaaatc atcccgagta tgccgacctt tccgctcagg attggtattg ctatgatact
     3241 tatccccaga tactggtggt ctcacactgg acactgctga gtcccgaata tgaaattcga
     3301 atctgccatc acaacaccat ctatcctcac aactggaccc aattcttact gagaaaaaga
     3361 ggtgaattcg atgctatgct cgcggtaaaa agggacaacg acagtacagc tttgcatgag
     3421 ataccccctt caaaatatcc cacacttttc ggctactgaa atagtacagg gttgggctga
     3481 cataagacgg tggtaatcaa aaccagtagg cagtattcaa aaagcgcttg actttacggg
     3541 aagataaagt aaattatagt cacctcattt ttttttaggg atgaattgca aaatat
//
LOCUS       DSEB01000484            2471 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1006346, whole genome shotgun sequence.
ACCESSION   DSEB01000484 DSEB01000000
VERSION     DSEB01000484.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1006346"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            <1..1396
                     /locus_tag="ENO07_08625"
     CDS             <1..1396
                     /locus_tag="ENO07_08625"
                     /inference="COORDINATES: protein motif:HMM:PF00115.18"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HDS02068.1"
                     /translation="YNMVLSMHATLMIFFVIIPALTGFGNYFVPLLIGARDMAFPRLN
                     AFSFWLAVPAAVLMFASFFVAGGATQAGWTAYPPLSLAAYSAGVGMDMWIMGIILIGL
                     SSILGAVNFIVTIANLRAPGMSWFKMPLFAWSWLVNASMILIATPVLSAAVSMLLADR
                     LFGTGFVTVGKGGDPLLYQHLFWFYSHPAVYIMILPGFGMISHVVAAFSRKKIFGYKG
                     MVIALALIGIIGYTVWAHHMFVSGMPAWLQVLFSYLTMIIAVPTGIKVFSWIATAWGG
                     SIRFTTSMKFALGFISVFIIGGMSGITLANVPVDYQVHDSYYVVAHFHYVLVGGSILA
                     LMAGLYYWFPKMSGRFLSEKLGRIVFWAIYLGLNVTFFPMHFMGIQGMARRIYTYRPE
                     FATLNAISSIGYIFMLVGGLLLVYDLVRNGIFRKKRGLMNDPWEVNDIQQTLEWEISS
                     PPPDYNFEKIPEIK"
     gene            1396..>2471
                     /locus_tag="ENO07_08630"
     CDS             1396..>2471
                     /locus_tag="ENO07_08630"
                     /inference="COORDINATES: protein motif:HMM:PF02628.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HDS02069.1"
                     /translation="MSVDTDMEDSALTTSQDTEKAYPPDKSLGRWFLWVSILVVFLVV
                     IGGIVRLTDSGLSIPEWPIINGSLLPPVTDDDWEAVYKTYHSVIEGVEVDSVYHNAYP
                     GIIPIGRFKQMFALEYFHRFVAAVLGILYLVLLIKVMRRREIRSRFGVRMWIGLALLI
                     LQAVMGGIVVKYDLQAEFLAVHLGLAFALFGLLYWTSMSLLRPPDEHKTYYNKFLSRI
                     AWSGVAALFLQVLSGAVMAGTKAGLSWNTWPLIGDHLIPPPSVMWRSYMGILNFIHNT
                     VLVQFIHRWWAFAALIVIVFLVFYSLKFKVTPRCRIAFRVITSVVALQIILGIITLLT
                     KVPPTLGVLHLTLGIILFGNMLYIT"
BASE COUNT          518 a          585 c          662 g          706 t
ORIGIN      
        1 ctataatatg gttctttcca tgcatgccac cttgatgatc ttctttgtaa tcatcccggc
       61 cctgaccggg ttcggcaact actttgtgcc gcttctaatc ggagcgcgcg acatggcctt
      121 cccgcgcctg aatgctttct cattctggct ggcggtccct gcggcggtgc tgatgtttgc
      181 cagttttttt gtggccggag gagccacgca agctggatgg acagcatatc cgccattatc
      241 gctggcggca tattcggccg gagtgggcat ggacatgtgg atcatgggga ttatcctgat
      301 cggactttct tctatcctgg gagcggtcaa tttcatcgtc accatcgcaa accttcgcgc
      361 tcccggtatg agctggttca agatgccatt atttgcctgg agctggctgg tgaacgcttc
      421 gatgatatta atagctaccc cggtattatc ggcagcagtt tccatgctcc tggcagacag
      481 gctcttcggt accggattcg taactgtcgg taagggtgga gacccgctgt tgtatcagca
      541 cctgttctgg ttctactcgc atcctgcggt gtatataatg attctgcccg ggtttggtat
      601 gatctcccat gttgtcgcgg cattttcgag aaaaaagatt tttggatata agggcatggt
      661 aatagcgctc gccctgatcg gaataatcgg ctacactgtc tgggcgcacc acatgtttgt
      721 atcgggcatg cccgcctggc tgcaggtgct cttcagctac ctgaccatga tcatcgcagt
      781 gccgaccgga atcaaagtct ttagctggat tgccaccgcc tggggcggct cgatacgatt
      841 cacgacctcc atgaaattcg cactgggatt tatttctgtc tttatcatcg gcgggatgag
      901 cgggattaca ctggccaatg tcccggtcga ttaccaagtg catgattcat actatgtggt
      961 tgctcatttc cattatgtgc tggtgggagg ctcgattctt gcacttatgg cgggattata
     1021 ttactggttc cccaagatgt caggaagatt tttgagcgag aagctggggc gaattgtatt
     1081 ctgggcgatt tatctgggac tgaatgttac cttctttccc atgcatttta tgggcattca
     1141 gggcatggcg cgaagaattt atacttatcg cccggaattt gcaacattga atgcgatctc
     1201 ctcgattggg tatattttta tgctggtggg aggactcctg ctcgtttatg atctggtaag
     1261 aaacggtatc ttcaggaaaa agcgcggctt gatgaatgat ccctgggagg tcaacgatat
     1321 tcagcagacg ttagaatggg aaatctccag tcccccgccg gactataatt ttgaaaaaat
     1381 accggagata aaataatgtc cgtggatact gacatggagg acagcgctct tacgaccagc
     1441 caagataccg agaaagctta tccgccggat aaaagtctcg gcaggtggtt tctgtgggtc
     1501 agcatcctgg tcgtgtttct ggtcgtgatc ggcggaatag tgcgtctgac agattcgggc
     1561 ctgtcgatcc cggaatggcc gattataaac ggttcgctcc tgccgcctgt aaccgatgat
     1621 gactgggagg ctgtttacaa aacctatcac agtgttatcg agggagtgga ggtcgacagc
     1681 gtctaccaca atgcctatcc cggtatcatt ccgatcggca ggttcaagca gatgtttgcg
     1741 ctggaatatt ttcaccgctt tgtggccgct gttctgggca ttctctatct tgtactgctg
     1801 ataaaagtta tgcgaagaag ggaaatacgt tcacgcttcg gcgtgcgcat gtggatcggc
     1861 ctggcgcttc tgatcctgca ggcggtgatg ggcggtatag tagtcaaata tgatctccag
     1921 gcggaatttc tggcagtgca tctggggctg gcattcgcgc tgttcgggct tctttactgg
     1981 acctcgatga gtctcctgag gcccccggat gaacataaga cttattacaa taaattcttg
     2041 agccgcatcg cctggtcagg agtggccgcg cttttcctgc aggttctttc cggggctgtg
     2101 atggccggta ccaaagccgg cctgagctgg aatacatggc ccttgatcgg cgatcacctg
     2161 atcccgccac caagcgtgat gtggcggtca tatatgggaa ttttaaattt catacataac
     2221 acggtgctgg tgcagtttat tcatagatgg tgggcctttg ccgcccttat cgtgattgtc
     2281 tttctcgtat tttattccct gaagttcaag gttactcccc gttgtcgaat cgctttcagg
     2341 gtgattacaa gcgtggttgc gctgcagata attctgggta taattacact tttaacaaaa
     2401 gtcccaccga ctttgggcgt actgcacctg accctgggaa ttatcttatt tggaaatatg
     2461 ctatatataa c
//
LOCUS       DSEB01000485            3424 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1003069, whole genome shotgun sequence.
ACCESSION   DSEB01000485 DSEB01000000
VERSION     DSEB01000485.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1003069"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..1651)
                     /locus_tag="ENO07_08635"
     CDS             complement(<1..1651)
                     /locus_tag="ENO07_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS02070.1"
                     /translation="MNMKNKKTTGLLLIAGFLMLLCGTLGAGVLEINELNKNEAEPDY
                     QRHPHGGFKSDVSQQNLLGLQRSGKSLLPETSLSAQAPLTLKMCAVRVQFQDESPDDD
                     STTGTGQFDFRTYEQFLEEEKHGIDPAPHNREYFEKHIEALNNYWHTVSNGNLTIEGT
                     VFPADSDSVYTLEHTMGYYGSQSPEFGLGELFYDALRLADRDDDINWIEYDVFVIFHA
                     GSDRQNDLGFPPTPHDLFTGFIIMGEPVPVEDSTILIMEGMIMPETASQDNRATALNA
                     VMAHEFGHQLGLVDLYDTHRFNTFVGDFSLMDNNGFGTGVDLGFELTRTILGTIPLYP
                     EAWSRAYLGFNDVVVVEEGINLDVMAAELASNTSKIYKVPISENEYFLIENRQIDPDR
                     DGGASLKADLDGTGVILGPAPNPDVVPPGQPAPLTREYDFLMPGSGMVIWHVDESVAW
                     LDYDNDGLNNFYDNDLQWYYIEGFDDQFEPWENRPFLRLIEADGIIDFGGEYWTNFGR
                     AEDMFEANRNDHFGPNTNPSTRSNSIAYTGIDIADISGNDTI"
     gene            complement(1667..>3424)
                     /locus_tag="ENO07_08640"
     CDS             complement(1667..>3424)
                     /locus_tag="ENO07_08640"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HDS02071.1"
                     /translation="WVGTDEGLYRYDTKEKEWRKFTEQSGLPSSKITSLSSPDNQSLW
                     VATPVGLALYERGSWQIFGEEQGIKDTGLYLVYADARNRVWAVSEDNFYRFNGAEWTD
                     YFVYEASVNDTIQKIMRDFIGYSDQNSLINAIYQTKMMNGLESDIPDPGQELKIPFAY
                     SLTHKINVMTYDSEGRLWVGTDYGVKIFYDGKFTLFGYKPYTAEKEMTVEEIAADFLD
                     TDNEVQIDQLARKIRDFNGISTNKIPAGQTVYISSNPLASKITSLMDDGSKVYIGTEK
                     GLVQYSDERFGYFYQEGLEKQRTIDIISRDGETWYATPNKVVVFASAKSEITGMHANW
                     LPELADDIYYEFLSYINHLGEEWGTVGINVTFLSYGELVRVNEYNEIMGTFNSFDMAI
                     GLSYGKKMSNSLSAGLTAKWIYSRLSDQGAGAEIGEGTGSSLGIDIGLLYRPAKRLTF
                     GAAVTNLGPDIAYIDAAQSDPLPRNLAFGLSYKLIDNPYNRLMLVAEINKMLTGLDDG
                     FSEELKEAIENVGFEYWYGSFIAFRAGYIYDQVGQVKTATLGVGIQYRGFRFDFAYIP
                     SSDTVPLANTVRFSLTGRI"
BASE COUNT          828 a          920 c          819 g          857 t
ORIGIN      
        1 taatagtatc gttgccgctg atatcggcaa tatctattcc agtataggcg attgaatttg
       61 acctggtgga gggattggta ttggggccga aatgatcgtt gcggttggcc tcgaacatat
      121 cttcggccct cccgaagttg gtccagtatt ctcctccgaa atcgataata ccgtcagctt
      181 cgatcaggcg cagaaacggc ctgttttccc agggctcgaa ctggtcgtca aaaccctcga
      241 tatagtacca ctgcaaatcg ttgtcataaa aattattgag gccgtcgtta tcataatcca
      301 gccacgccac cgattcatcc acatgccaga ttaccatacc ggaaccgggc atcagaaaat
      361 catattcacg ggtcaggggg gcgggctgtc cgggcggaac cacatcggga ttgggcgcag
      421 ggcccaggat taccccggta ccgtcaagat cggccttgag gctcgctccg ccgtcacggt
      481 cggggtcaat ctgccggttt tcaataagaa aatattcatt ttccgaaatc ggcaccttgt
      541 aaatcttgct tgtattggag gccaattcgg ccgccataac atccagattg atcccctctt
      601 caaccacaac aacatcattg aaaccgagat aggcacgcga ccaagcttcc gggtagagcg
      661 ggattgtgcc caatatagta cgggtaagtt caaaacccag gtccaccccg gtgccgaaac
      721 cgttattatc catcagcgag aaatcgccca cgaaagtgtt gaacctgtgt gtgtcgtaca
      781 ggtcaaccag gcccagttga tggccaaact cgtgggccat taccgcattc agcgccgtgg
      841 cgcggttatc ctgcgaggcc gtctccggca ttatcatacc ttccattatc agtattgtac
      901 tgtcttcaac cggcacaggc tcacccataa tgatgaatcc ggtgaagaga tcatgcggag
      961 tgggcgggaa tcccaggtcg ttctgcctgt cgctgccggc atgaaaaatc acaaagacgt
     1021 catactcaat ccagttgata tcatcatccc ggtcggccag cctcagggca tcataaaaca
     1081 actcgcccaa accgaactcc gggctctgcg aaccatagta acccatagtg tgctcgagcg
     1141 tgtagacgct gtctgaatca gccggaaaca ccgttccctc gatcgtcaga ttgccgtttg
     1201 aaacagtgtg ccagtaatta ttcagcgctt caatatgctt ctcgaagtat tcgcggttat
     1261 ggggggccgg atctatgcca tgcttctctt cttccagaaa ttgctcgtag gtgcggaaat
     1321 cgaactgccc tgttccggta gtggaatcat catccgggct ttcatcctgg aattgtaccc
     1381 gcaccgcaca catcttcaat gtcagcggag cctgggccga aagcgaggtc tccggcagga
     1441 gcgatttgcc gctgcgctga agtcccagca aattctgctg agaaacatca ctcttgaatc
     1501 cgccgtgagg atgacgctga taatctggct ctgcctcatt cttattcagt tcgtttatct
     1561 ccagtacccc ggccccaaga gtgccgcaga ggagcatcag aaatcccgca attaaaagca
     1621 gccccgttgt ctttttattc ttcatgttca tcaaccgtat ggactcctat attcttcctg
     1681 tcaacgagaa tctaactgta tttgccaggg gcactgtatc cgaggacggt atgtatgcga
     1741 aatcaaatct gaatccgcga tactgaatac caactccgag cgttgcggtc ttgacctggc
     1801 ccacctggtc atatatgtag ccggcgcgga aggctatgaa ggaaccgtac cagtattcga
     1861 atcccacgtt ctcgattgcc tccttgagct cctccgagaa tccgtcgtcc agtccggtca
     1921 gcatcttatt gatctcggca accagcatga gacgattata gggattgtct atgagtttat
     1981 atgaaaggcc gaacgccagg tttctcggca gcgggtcgga ctgggccgca tcgatataag
     2041 ctatatcggg acctaaattg gtcaccgccg ctccaaatgt caggcgtttg gccggccggt
     2101 ataaaagacc gatatcgatt cccagcgaag atcccgtacc ctctccaatc tccgccccgg
     2161 cgccctgatc ggaaaggcgc gagtatatcc atttggcggt aagaccggcg gaaagactat
     2221 tggacatctt cttgccgtag ctcaggccga tagccatatc aaacgaatta aaagtaccca
     2281 tgatttcatt gtattcattg acgcggacaa gttcaccata actcaggaag gtaacattta
     2341 tgcccacagt accccattcc tcacccagat gattaatata ggaaaggaat tcatagtata
     2401 tgtcatcagc aagctccggc agccagttgg catgcatacc tgttatctcc gacttggccg
     2461 aagcgaaaac cacaacctta ttgggtgtgg cataccaagt ttcgccgtcc ctggatataa
     2521 tatcgatagt tctttgtttt tccagccctt cctggtagaa gtagccaaac cgctcgtcgg
     2581 aatactggac cagtcccttt tcggttccga tgtagacctt ggaaccatca tccatcaagg
     2641 atgtgatttt gctggccagc ggattggaag agatatacac agtttgaccg gccggaatct
     2701 tgttggttga aattccgttg aaatcgcgga tttttctcgc cagctgatca atctggacct
     2761 cattgtcggt atccagaaaa tcggccgcga tttcctcgac cgtcatttcc ttttcagcag
     2821 tgtagggctt ataaccgaag agcgtgaact ttccgtcata gaatattttc accccgtaat
     2881 cggttccgac ccatagacgg ccctcggaat cataggtcat aacgttaatc ttgtgtgtca
     2941 gggagtaagc gaacgggatt ttcagttcct ggcccggatc ggggatatcc gactcaagtc
     3001 cattcatcat cttagtctgg tagatggcgt ttatcaggga attctgatcg gaatagccga
     3061 taaagtcacg cattatcttc tgaattgtat cattaacaga ggcctcgtag acaaaatagt
     3121 cggtccattc agcaccgtta aacctgtaga aattgtcctc tgaaaccgcc cagactctat
     3181 tccttgcgtc ggcatagacc aggtagagac cggtgtcttt aattccctgt tcctctccaa
     3241 agatctgcca tgacccgcgc tcgtacaagg ccaatccgac cggagtggcc acccacagcg
     3301 attgattgtc gggggacgag agagaagtta ttttgctcga ggggagcccg ctttgttcag
     3361 tgaacttgcg ccactctttc tccttggtat catagcgata caggccctcg tccgtgccga
     3421 ccca
//
LOCUS       DSEB01000486            1974 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1010389, whole genome shotgun sequence.
ACCESSION   DSEB01000486 DSEB01000000
VERSION     DSEB01000486.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1010389"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            1560..1871
                     /locus_tag="ENO07_08645"
     CDS             1560..1871
                     /locus_tag="ENO07_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS02072.1"
                     /translation="MPQLALNLSGFVAKNTSAFEQVDMPEPEEAVEHLDYTFEDMHKY
                     SDLNYLNYQIGGGATYSFENGIKWTVDVTYYGLDDRLGYVYGDETGSLVVVRSGVQLD
                     F"
BASE COUNT          505 a          447 c          499 g          523 t
ORIGIN      
        1 actatatttt gttcaggcgg cagcttcaga ccgatcttct gaatgacatc aatgcccgtg
       61 aaatgctggc cgatggatca ccgggcggaa aaatgctaac tcatgaggac ttcgatcccg
      121 gttttgattt caattacgac cggcacaggg ttgaatcgaa gatggaattt ttgatcgacg
      181 agaaaaacga tattcgggtt accgcggccc atcgcttctg gaatgattcc gggcatgagc
      241 agagagtggc ctcgatgcat tgttttagct gtcatctgac gtcgcaggcg gtcgaagttg
      301 atcgaaccgt aaatcagttc aatcttggaa ttgaaggtaa gccgggcccg gtaggtgtca
      361 gctataattt catctacagc aaattcagct ctgatgctcc cgcgccgagt atcttctatg
      421 atgaggccaa gcatccggtt aacggcggtt cagctgagga atttgattca cgtgtgatct
      481 atgccggtga agatgttata tacgcaactt atcccgaaac cgagaaaatc ggcaacaaag
      541 tcaggttcag ttcggatata gggaagcacc gattgagcgg ttccgctttt tatcaccgtt
      601 cccagaatga gctttctgac cttgagaatc aaataattgc gggtcaatat gataacctgg
      661 tttccgacac ttatggcgga acccttaatt tcagcgccct tctcaacaaa accatgcgtc
      721 ttctggcaaa aacatcggtc tccagaacaa aggccgacga tatcatggtc gaattaccgc
      781 tctggcgcga agatcgtccg ggcggagatc aggatttcag tttcatgcgc tattcttctc
      841 tggatcggct caggggggat gccagcctgg agatgatcat gcgcctgaat ccgcagacca
      901 ctttatcaat cctcggagga tatcaaggcg tcaggcgtga cgactatcca tacaaggatg
      961 ctgatgacgc taccaaccgc ctgataggtc aggccaaggt gcgttaccgt aaaggcctgg
     1021 aatattcgct gggattcaag tacaggctgg agaaaaccta tagccccttc accagttacc
     1081 gtgggctttt cgaagcctcc ggccgagagg aagggctctc tcccctcgaa ggaaatacat
     1141 tcatttttta tttccagcgt gaggacctga aataccagga tgttacctca ttgccgacaa
     1201 ttgaacatga cctggaactg accggcaatc tgaacctgaa cagtaaagtc agccttaatg
     1261 ccaatgttca attgagattg gaccagaata atgatttaga cagcctcgaa gtagaacata
     1321 cgttcttcca gccttcattg ggctttaata tcatgccgac catggaatgg gtcttttccg
     1381 gaggaatgcg ctaccagatt ctcaaatcac gagggccggt gacggtggcc atgtttgatg
     1441 ggtgagcggg ccatgtattt ggtgactccg gtggagtcct tcattttggc tctctttata
     1501 cagatgctga ttacgaaagc gaagtacaga gcatatatgg ctctgtggca tacattccaa
     1561 tgccccaact cgctttgaat ctcagcggtt ttgtggcaaa gaatacttcc gcctttgagc
     1621 aagtcgatat gcccgaaccg gaagaggcgg ttgaacatct ggattatact ttcgaggata
     1681 tgcataaata ttcagatctg aactatctga attaccagat tggcgggggc gcaacataca
     1741 gttttgagaa cggcattaaa tggacagtcg atgtgaccta ttatggcctt gatgacaggc
     1801 tgggttatgt ttacggcgat gagaccggtt cgcttgttgt agttcgctct ggggtgcaac
     1861 tggattttta gaaattctct cactatatga cgtaagtgaa agcgcccgtg ggaattaaat
     1921 gcacgggcgt ttttttgttc aggcaatttt tttcagaatc cctttggata tatc
//
LOCUS       DSEB01000487            2429 bp    DNA     linear   ENV 02-MAR-2020
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate SpSt-1232
            Ga0116197_1006584, whole genome shotgun sequence.
ACCESSION   DSEB01000487 DSEB01000000
VERSION     DSEB01000487.1
DBLINK      BioProject: PRJNA480137
            BioSample: SAMN09639154
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (hot springs metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Zhou,Z., Liu,Y., Xu,W., Pan,J., Luo,Z.H. and Li,M.
  TITLE     Genome- and Community-Level Interaction Insights into Carbon
            Utilization and Element Cycling Functions of Hydrothermarchaeota in
            Hydrothermal Sediment
  JOURNAL   mSystems 5 (1), e00795-19 (2020)
   PUBMED   31911466
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Zhou,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2018) School of Biological Sciences, The
            University of Hong Kong, Kadoorie Biological Sciences Building,
            Hong Kong, Hong Kong
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. 1.1.2
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 25.78x
            Sequencing Technology  :: Illumina HiSeq 2500
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/26/2019 17:00:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 1,732
            CDSs (total)                      :: 1,714
            Genes (coding)                    :: 1,700
            CDSs (with protein)               :: 1,700
            Genes (RNA)                       :: 18
            tRNAs                             :: 18
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 6 of 14
            Pseudo Genes (incomplete)         :: 4 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Ga0116197_1006584"
                     /isolate="SpSt-1232"
                     /isolation_source="hot spring sediment"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /country="USA: Zodletone Spring, Oklahoma"
                     /lat_lon="34.9956 N 98.6889 W"
                     /collection_date="2013-12-03"
                     /metagenome_source="hot springs metagenome"
                     /note="Metagenome-assembled genome binned from sequencing
                     reads available in SRR5214155;
                     metagenomic"
     gene            complement(<1..632)
                     /locus_tag="ENO07_08650"
     CDS             complement(<1..632)
                     /locus_tag="ENO07_08650"
                     /inference="COORDINATES: protein motif:HMM:PF01523.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS02073.1"
                     /translation="MGQESFRGMPINDLYSLALDEASRLGATYADYREEDITEQSLSV
                     SDGSPELLNKSFSTGFAIRVIADGAWGFAADSIINEDSIRKTAATAVEIAKASARINK
                     SALELAERQVVEDAYATPFKKDPFAVPLSEKIEFLSELERMMRIDSQINSTSAFMTFI
                     RTQKFYASLDGSRISQDIYQAGCGASCGVIRSHRDRSERSFPTPNGQWITG"
     gene            complement(613..1536)
                     /locus_tag="ENO07_08655"
     CDS             complement(613..1536)
                     /locus_tag="ENO07_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDS02074.1"
                     /translation="MRSRWPDGGMMKPYKIFLALLFTLAIIIGIRYTNQNYNQIEKKA
                     YGDGFILAHEFNHICYNEDRDVIISVEVEDGSLPAGSSLGFFYKPARLSGYEGEIGYT
                     RYEMKQALAYPDRYEIIIPRGEKGDRVLYYLELNDSEGNEIALLTSEQGAESTEETRL
                     MFAGQRPRYLFLGHVIVMLAGFVFVSLAFLTALSNLKDKRNNTILGKQIFLAAIFIFV
                     GLVPLGSLLNNQMYGTYWKGLPFGIDITGSLGFIILLYWLIMMILMKGSAFVSNPSKN
                     ILPPSGARVLAVIGFLITAAAYLVPHGTGEF"
     gene            complement(1517..>2429)
                     /locus_tag="ENO07_08660"
     CDS             complement(1517..>2429)
                     /locus_tag="ENO07_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDS02075.1"
                     /translation="YQTIKDDEKVRREESDKELLEMIHAELEELRPRLEELEEQLKLL
                     LVPRDPNDAKNTIMEIRAGTGGEEAALFAADLFRMYQRYIEENGWRLEVMTSNPTGVG
                     GFKEIIFLVEGDGAYGNLKFESGVHRVQRVPETEASGRIHTSAASVAVLPEAEDVDID
                     IDPNDLRIDVYRSSGPGGQSVNTTDSAVRITHVPTGLVVSCQDEKSQLKNKNKAMKVL
                     RARLLDKATAEQNAARASQRKAQVSTGDRSAKIRTYNFPQGRLTDHRIGLTLYKLDSI
                     MEGELDDVIEALKLEDRAERLNEIEVA"
BASE COUNT          579 a          662 c          552 g          636 t
ORIGIN      
        1 ccggtaatcc attgaccatt gggggtgggg aatgatctct cgctcctgtc cctgtgtgat
       61 ctgattacac cgcatgaggc cccgcatcca gcctgataaa tatcctggct gattcgggag
      121 ccatccaggg aggcatagaa tttctgagtt ctgataaatg tcatgaaggc ggaggtcgaa
      181 ttgatctgtg aatcgatccg catcatcctt tccagctcgc tcaaaaactc gatcttttct
      241 gataacggca ctgcgaacgg gtccttttta aagggggtgg cgtaagcatc ttcgaccacc
      301 tgtctttcag ccaattccag tgctgattta tttatcctgg ccgaggcttt ggctatctca
      361 actgcagttg cggcggtctt ccttatagag tcctcattaa taatcgaatc agccgcaaat
      421 ccccaggcgc cgtcagcgat tacccttatg gcaaagccgg tagagaatga cttattcaga
      481 agctcgggag aaccatcaga gacggacaag ctctgttcgg ttatgtcctc ctcgcgataa
      541 tcggcatacg tcgcccccag gcggctggcc tcatcaaggg cgagactgta tagatcattt
      601 atgggcattc ctctaaaact ctcctgtccc atgcgggacc agataggccg cggcagtgat
      661 cagaaagcca attacagcaa gaacccgcgc tcccgaggga ggcaggatat tcttggatgg
      721 attggacaca aaggccgagc ctttcatcaa gatcatcatg atcagccagt aaagcaatat
      781 aataaatccc aggctgccgg ttatatcaat tccaaatggc agccccttcc agtaggttcc
      841 atacatctga ttatttaaca aacttcccag cggtacgagc ccgacaaaaa tgaaaattgc
      901 agcaaggaaa atctgttttc cgagaatcgt attattccgc ttatctttaa ggtttgataa
      961 tgccgtcagg aatgcgagag acacaaaaac gaaacccgcc agcataacaa taacatgccc
     1021 taaaaacaaa tagcgaggtc tttggccggc aaacatcagc cgtgtttcct cggtgctctc
     1081 cgccccctgt tccgacgtca gcaaagcaat ctcatttcct tcactgtcat tcaattccag
     1141 ataataaagg accctgtccc ctttttcacc gcgcggaata atgatttcat agcgatccgg
     1201 ataggcgagg gcctgcttca tctcataccg ggtataccca atttcacctt cgtatccgga
     1261 cagccgtgcc ggtttgtaga aaaagccgag gctgcttcct gcgggcaggc ttccgtcctc
     1321 gacttcgaca gaaattataa catcccgatc ttcattgtag cagatgtgat tgaattcatg
     1381 agccaggata aagccatcac cataggcttt tttctcgatt tgattataat tttggttggt
     1441 gtatctgatt ccgattatta ttgccaatgt aaacaggagc gcaagaaaaa tcttgtacgg
     1501 cttcatcatc cccccgtcag gccacctcga tctcattcag tctttcggcg cggtcctcga
     1561 gctttaatgc ctcaataacg tcgtccagtt cgccttccat tatagaatca agtttataaa
     1621 gagtcaatcc aattctgtga tcggtcagcc tcccctgagg gaagttatat gtcctgatct
     1681 tggctgatct gtcccctgtc gatacctgtg cctttcgctg gctggctctt gcggcatttt
     1741 gctcggcagt ggccttatcc agtaaacggg cccttaaaac cttcatggcc ttattcttgt
     1801 ttttcaattg cgacttttca tcctgacagc tcaccaccag ccctgtgggg acatgtgtga
     1861 tcctgacagc ggagtcggtc gtattaacag actgtccgcc cgggccggat gaccggtaaa
     1921 catctatacg aaggtcattc ggatcgatat cgatatcgac atcctctgcc tcgggaagca
     1981 ccgctactga cgctgcagaa gtgtggattc tcccggaggc ctccgtttcg ggcacgcgct
     2041 ggacacgatg tacccccgat tcgaatttca gattgccgta ggcgccgtca ccctcgacca
     2101 gaaagattat ctccttgaat ccacctactc cagttggatt ggaggtcatg acctccagtc
     2161 tccagccgtt ctcctcaata tagcgctggt acatacggaa aagatcggcc gcaaagaggg
     2221 ccgcctcctc gccgccggta ccggcacgga tttccataat cgtatttttg gcatcattgg
     2281 gatctcttgg caccaacagg agcttaagct gttcttccag ctcctcgagc ctgggacgaa
     2341 gttcctcgag ctcggcatga atcatctcca gaagctcctt atccgattcc tcgcgtctga
     2401 ccttttcatc atccttgata gtctgatag
//
